organism: Fusarium solani


enzyme_display_name: carboxylesterase

organism: Homo sapiens

substrate_name: cis-Permethrin; H2O

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

km_unit: mM

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

enzyme: carboxylesterase

substrate: cis-Permethrin; H2O


organism: Mus musculus

substrate_name: H2O; cis-Permethrin

substrate: H2O; cis-Permethrin


organism: Oncorhynchus mykiss

substrate_name: H2O; Trioleoylglycerol

km_display: Not reported

substrate: H2O; Trioleoylglycerol


organism: Rattus norvegicus

substrate_name: H2O; trans-Permethrin

substrate: H2O; trans-Permethrin


organism: Vibrio vulnificus

substrate_name: p-Nitrophenyl acetate; H2O

protein_accession_database: UniProt

sequence_source: UniProtKB reviewed accession Q8DF91

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

substrate: p-Nitrophenyl acetate; H2O


organism: Bacillus sp. (in: firmicutes)

substrate_name: D-fructose 6-phosphate

source_db: brenda

verification_status: unverified

source_license: CC BY 4.0

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

data_origin: experimental

substrate: D-fructose 6-phosphate

status: unverified

source: brenda


enzyme_display_name: UniProt Q2FXM8

organism: Staphylococcus aureus

source_db: skid

source_license: CC BY-NC-ND 4.0

enzyme: UniProt Q2FXM8

source: skid


organism: Saccharomyces cerevisiae

substrate_name: ATP

source_db: oed

kcat_over_km_origin: calculated

substrate: ATP

source: oed


substrate_name: D-Fructose 6-phosphate

substrate: D-Fructose 6-phosphate


enzyme_display_name: ATP-dependent 6-phosphofructokinase

organism: Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM)

source_db: uniprot

enzyme: ATP-dependent 6-phosphofructokinase

source: uniprot


substrate_name: GTP

substrate: GTP


substrate_name: ITP

substrate: ITP


substrate_name: UTP

substrate: UTP


substrate_name: CTP

substrate: CTP


substrate_name: fructose 6-phosphate

smiles: C(C(C(C(C(=O)CO)O)O)O)OP(=O)(O)O

substrate: fructose 6-phosphate


organism: Bombus atratus

substrate_name: ATP; D-Fructose 6-phosphate

assay_conditions_summary: cofactor ATP

substrate: ATP; D-Fructose 6-phosphate


assay_conditions_summary: pH 8; 303.15 K; cofactor ATP


organism: Dunaliella marina

substrate_name: D-Fructose 6-phosphate; ATP

substrate: D-Fructose 6-phosphate; ATP


assay_conditions_summary: pH 7; cofactor ATP


organism: Desulfurococcus amylolyticus


assay_conditions_summary: pH 6; 323.15 K; cofactor ATP


substrate_name: ADP

assay_conditions_summary: pH 6; 358.15 K; cofactor ADP

substrate: ADP


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution


substrate_name: D-fructose 1,6-bisphosphate

substrate: D-fructose 1,6-bisphosphate


organism: Auxenochlorella pyrenoidosa


assay_conditions_summary: pH 7.7; cofactor ATP


organism: Trachemys scripta


assay_conditions_summary: pH 7; 279.15 K; cofactor ATP

proof_lines: pH 7.0, 20°C, normoxic and anoxic animals


proof_lines:
pH 7.0, 6°C, anoxic animals
pH 7.0, 20°C, normoxic animals
pH 7.0, 6°C, normoxic animals


organism: Kluyveromyces lactis


ki_unit: mM


organism: Pichia pastoris


organism: Haloferax volcanii

assay_conditions_summary: pH 8; 315.15 K; cofactor ATP


organism: Austromegabalanus psittacus


organism: Pseudomonas doudoroffii

proof_lines: SABIO-RK modifier species: 6-phosphofructokinase(Enzyme) mutant M-1


organism: Blastocladiella emersonii


organism: Monoraphidium minutum

assay_conditions_summary: pH 7.25; 298.15 K; cofactor ATP


organism: Zapus hudsonius


organism: Selenastrum minutum


enzyme_display_name: DNA-directed DNA polymerase

organism: [Bacillus] caldotenax

substrate_name: dNTP

enzyme: DNA-directed DNA polymerase

substrate: dNTP


enzyme_display_name: UniProt P19821

organism: Thermus aquaticus

substrate_name: dATP

enzyme: UniProt P19821

substrate: dATP


enzyme_display_name: UniProt P96022

organism: Saccharolobus solfataricus

substrate_name: dTTP

enzyme: UniProt P96022

substrate: dTTP


organism: Thermococcus kodakarensis

substrate_name: D-glucose

substrate: D-glucose


substrate_name: AMP

substrate: AMP


assay_conditions_summary: pH 7.5; 323.15 K; cofactor ADP


organism: Geobacillus stearothermophilus

sequence_source: multiple

assay_conditions_summary: pH 8.2; 303.15 K; cofactor ATP

source_databases_merged: brenda uniprot


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_ranked_match and applied mutation signature G212V to reconstruct the assayed variant sequence.


substrate_name: D-fructose 6-phosphate

substrate: D-fructose 6-phosphate


substrate_name: ATP

substrate: ATP


source_license: CC BY 4.0; CC BY-NC-ND 4.0

proof_lines:
pH 8.2, 30°C, native PFK
Raw SKiD Env: Temp=25°C, pH=8

source_databases_merged: brenda skid


organism: Escherichia coli

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature E190Q to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 8.2; cofactor ATP

proof_lines: mutant enzyme E190Q, in the presence of Mn2+, in 0.05 M Tris-HCl, pH 8.2, temperature not specified in the publication

proof_lines: mutant enzyme E190Q, in the presence of Mg2+, in 0.05 M Tris-HCl, pH 8.2, temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from uniprot_accession_plus_mutation_signature_reconstruction and applied mutation signature I153V to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession_plus_mutation_signature_reconstruction and applied mutation signature I150V to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession_plus_mutation_signature_reconstruction and applied mutation signature I234V to reconstruct the assayed variant sequence.


substrate_name: dGTP

substrate: dGTP


substrate_name: dCTP

substrate: dCTP


organism: Saccharomyces cerevisiae

substrate_name: DNA; dGTP

substrate: DNA; dGTP


organism: Bacillus methanolicus

assay_conditions_summary: pH 9; 303.15 K; cofactor ATP

proof_lines:
strain PB1, Pfk2, pH 9.0, 30°C
strain MGA3, Pfk1, pH 9.0, 30°C
strain MGA3, Pfk2, pH 9.0, 30°C


proof_lines:
strain PB1, Pfk2, pH 9.0, 30°C
strain MGA3, Pfk2, pH 9.0, 30°C
strain MGA3, Pfk1, pH 9.0, 30°C


organism: Abax parallelepipedus


organism: Tequatrovirus T4

substrate_name: Unknown

substrate: Unknown


assay_conditions_summary: pH 7.2; 303.15 K; cofactor ATP

proof_lines: pH 7.2, 30°C, cosubstrate gamma-thio-ATP


proof_lines:
pH 7.2, 30°C, cosubstrate ATP
pH 7.2, 30°C, at 2mM ATP
Raw SKiD Env: Temp=30°C, pH=7.2


organism: Homo sapiens

assay_conditions_summary: pH 7.1; 298.15 K; cofactor ATP

proof_lines: wild-type, presence of 5 mM AMP, pH 7.1, 25°C


mutation_signature: N-terminal truncation mutant

sequence_variant_note: Mutation context was captured, but the signature could not be translated into an exact residue substitution safely.

mutation_type: truncation

proof_lines: N-terminal truncation mutant, pH 7.1, 25°C

proof_lines: N-terminal truncation mutant, presence of 5 mM AMP, pH 7.1, 25°C


organism: Staphylococcus aureus


sequence_variant_note: Resolved canonical sequence from canonical_uniprot_q2fxm8_plus_g150d_l151a_reconstruction and applied mutation signature G150D/L151A to reconstruct the assayed variant sequence.


organism: Aeropyrum pernix

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

assay_conditions_summary: pH 6.5; 368.15 K; cofactor ATP

proof_lines:
pH 6.5, 95°C, discontinuous assay
pH 6.7, 85°C


assay_conditions_summary: pH 7.2; 298.15 K; cofactor ATP

proof_lines: pH 7.2, 25°C, red blood cell lysate from diabetic subjects as source of enzyme


proof_lines: pH 7.2, 25°C, red blood cell lysate as source of enzyme


proof_lines: pH 7.2, 30°C, cosubstrate fructose 1-phosphate


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature D127S with inferred residue-numbering offset -11 to reconstruct the assayed variant sequence.


assay_conditions_summary: pH 6.7; 358.15 K; cofactor ATP

proof_lines:
pH 6.7, 85°C
pH 6.5, 95°C, discontinuous assay


proof_lines: in the presence of 0.5 mM MgATP2-, at pH 8.2 and 25°C

proof_lines: in the presence of 0.1 mM MgATP2-, at pH 8.2 and 25°C


organism: Fasciola hepatica

assay_conditions_summary: pH 8

proof_lines:
pH 8.0, native PFK
pH 8.0, phosphorylated PFK


proof_lines: pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G

proof_lines: pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG


verification_status: corrected

assay_conditions_summary: pH 7.5; 310.15 K; buffer 50 mM Tris-HCl

column_label: TABLE 2 (Steady-state kinetics of dNTP incorporation opposite G by Dpo 1, Dpo2, Dpo3, and Dpo4); Polymerase column = Dpo3; dNTP column = C (dCTP); column 'Km (uM)' = 75 +/- 13; column 'kcat (min^-1)' = 0.00053 +/- 0.00006; column 'kcat/Km (min^-1 uM^-1)' = 7.1 x 10^-6; column 'fins' = 1.

normalized_value_unit_evidence: Km: printed TABLE 2 cell 75 uM; 75 / 1000 = 0.075 mM. kcat: printed TABLE 2 cell 0.00053 min^-1, under the column head 'kcat' whose unit line reads 'min' with the superscript -1; one min^-1 is 1/60 s^-1, so 0.00053 / 60 = 0.000008833333333333332 s^-1. kcat/Km: printed TABLE 2 cell 7.1 x 10^-6 min^-1 uM^-1, under the column head 'kcat/Km' whose unit line reads 'min' with the superscript -1 followed by 'uM' with the superscript -1; one min^-1 uM^-1 is 1000/60 s^-1 mM^-1, so 0.000007099999999999999 x 1000 / 60 = 0.00011833333333333333 s^-1 mM^-1.

raw_value_unit_evidence: TABLE 2, Polymerase Dpo3, dNTP row C (dCTP): Km = 75 uM; kcat = 0.00053 min^-1; printed kcat/Km = 7.1 x 10^-6 min^-1 uM^-1.

row_label: TABLE 2, Polymerase block 'Dpo3', dNTP row 'C' (incoming nucleotide dCTP), insertion opposite template G

table_label: TABLE 2: Steady-state kinetics of dNTP incorporation opposite G by Dpo 1, Dpo2, Dpo3, and Dpo4 (J. Biol. Chem. 2011, 286(36):31180-31193)

status: corrected


column_label: TABLE 2 (Steady-state kinetics of dNTP incorporation opposite G by Dpo 1, Dpo2, Dpo3, and Dpo4); Polymerase column = Dpo1; dNTP column = A (dATP); column 'Km (uM)' = 2100 +/- 1300; column 'kcat (min^-1)' = 0.0018 +/- 0.0004; column 'kcat/Km (min^-1 uM^-1)' = 8.6 x 10^-7; column 'fins' = 3.2 x 10^-5.

normalized_value_unit_evidence: Km: printed TABLE 2 cell 2100 uM; 2100 / 1000 = 2.1 mM. kcat: printed TABLE 2 cell 0.0018 min^-1, under the column head 'kcat' whose unit line reads 'min' with the superscript -1; one min^-1 is 1/60 s^-1, so 0.0018 / 60 = 0.000029999999999999997 s^-1. kcat/Km: printed TABLE 2 cell 8.6 x 10^-7 min^-1 uM^-1, under the column head 'kcat/Km' whose unit line reads 'min' with the superscript -1 followed by 'uM' with the superscript -1; one min^-1 uM^-1 is 1000/60 s^-1 mM^-1, so 0.0000008599999999999999 x 1000 / 60 = 0.000014333333333333332 s^-1 mM^-1.

raw_value_unit_evidence: TABLE 2, Polymerase Dpo1, dNTP row A (dATP): Km = 2100 uM; kcat = 0.0018 min^-1; printed kcat/Km = 8.6 x 10^-7 min^-1 uM^-1.

row_label: TABLE 2, Polymerase block 'Dpo1', dNTP row 'A' (incoming nucleotide dATP), insertion opposite template G


column_label: TABLE 2 (Steady-state kinetics of dNTP incorporation opposite G by Dpo 1, Dpo2, Dpo3, and Dpo4); Polymerase column = Dpo1; dNTP column = C (dCTP); column 'Km (uM)' = 8.4 +/- 0.7; column 'kcat (min^-1)' = 0.23 +/- 0.004; column 'kcat/Km (min^-1 uM^-1)' = 2.7 x 10^-2; column 'fins' = 1.

normalized_value_unit_evidence: Km: printed TABLE 2 cell 8.4 uM; 8.4 / 1000 = 0.0084 mM. kcat: printed TABLE 2 cell 0.23 min^-1, under the column head 'kcat' whose unit line reads 'min' with the superscript -1; one min^-1 is 1/60 s^-1, so 0.23 / 60 = 0.0038333333333333336 s^-1. kcat/Km: printed TABLE 2 cell 2.7 x 10^-2 min^-1 uM^-1, under the column head 'kcat/Km' whose unit line reads 'min' with the superscript -1 followed by 'uM' with the superscript -1; one min^-1 uM^-1 is 1000/60 s^-1 mM^-1, so 0.027000000000000003 x 1000 / 60 = 0.45000000000000007 s^-1 mM^-1.

raw_value_unit_evidence: TABLE 2, Polymerase Dpo1, dNTP row C (dCTP): Km = 8.4 uM; kcat = 0.23 min^-1; printed kcat/Km = 2.7 x 10^-2 min^-1 uM^-1.

row_label: TABLE 2, Polymerase block 'Dpo1', dNTP row 'C' (incoming nucleotide dCTP), insertion opposite template G


column_label: TABLE 2 (Steady-state kinetics of dNTP incorporation opposite G by Dpo 1, Dpo2, Dpo3, and Dpo4); Polymerase column = Dpo1; dNTP column = G (dGTP); column 'Km (uM)' = 2500 +/- 900; column 'kcat (min^-1)' = 0.0068 +/- 0.0011; column 'kcat/Km (min^-1 uM^-1)' = 2.7 x 10^-6; column 'fins' = 1.0 x 10^-4.

normalized_value_unit_evidence: Km: printed TABLE 2 cell 2500 uM; 2500 / 1000 = 2.5 mM. kcat: printed TABLE 2 cell 0.0068 min^-1, under the column head 'kcat' whose unit line reads 'min' with the superscript -1; one min^-1 is 1/60 s^-1, so 0.0068 / 60 = 0.00011333333333333333 s^-1. kcat/Km: printed TABLE 2 cell 2.7 x 10^-6 min^-1 uM^-1, under the column head 'kcat/Km' whose unit line reads 'min' with the superscript -1 followed by 'uM' with the superscript -1; one min^-1 uM^-1 is 1000/60 s^-1 mM^-1, so 0.0000027 x 1000 / 60 = 0.000045 s^-1 mM^-1.

raw_value_unit_evidence: TABLE 2, Polymerase Dpo1, dNTP row G (dGTP): Km = 2500 uM; kcat = 0.0068 min^-1; printed kcat/Km = 2.7 x 10^-6 min^-1 uM^-1.

row_label: TABLE 2, Polymerase block 'Dpo1', dNTP row 'G' (incoming nucleotide dGTP), insertion opposite template G


column_label: TABLE 2 (Steady-state kinetics of dNTP incorporation opposite G by Dpo 1, Dpo2, Dpo3, and Dpo4); Polymerase column = Dpo3; dNTP column = T (dTTP); column 'Km (uM)' = 930 +/- 160; column 'kcat (min^-1)' = 0.00011 +/- 0.00001; column 'kcat/Km (min^-1 uM^-1)' = 1.2 x 10^-7; column 'fins' = 1.7 x 10^-2.

normalized_value_unit_evidence: Km: printed TABLE 2 cell 930 uM; 930 / 1000 = 0.93 mM. kcat: printed TABLE 2 cell 0.00011 min^-1, under the column head 'kcat' whose unit line reads 'min' with the superscript -1; one min^-1 is 1/60 s^-1, so 0.00011 / 60 = 0.0000018333333333333333 s^-1. kcat/Km: printed TABLE 2 cell 1.2 x 10^-7 min^-1 uM^-1, under the column head 'kcat/Km' whose unit line reads 'min' with the superscript -1 followed by 'uM' with the superscript -1; one min^-1 uM^-1 is 1000/60 s^-1 mM^-1, so 0.00000012 x 1000 / 60 = 0.000002 s^-1 mM^-1.

raw_value_unit_evidence: TABLE 2, Polymerase Dpo3, dNTP row T (dTTP): Km = 930 uM; kcat = 0.00011 min^-1; printed kcat/Km = 1.2 x 10^-7 min^-1 uM^-1.

row_label: TABLE 2, Polymerase block 'Dpo3', dNTP row 'T' (incoming nucleotide dTTP), insertion opposite template G


column_label: TABLE 2 (Steady-state kinetics of dNTP incorporation opposite G by Dpo 1, Dpo2, Dpo3, and Dpo4); Polymerase column = Dpo1; dNTP column = T (dTTP); column 'Km (uM)' = 3100 +/- 600; column 'kcat (min^-1)' = 0.096 +/- 0.008; column 'kcat/Km (min^-1 uM^-1)' = 3.1 x 10^-5; column 'fins' = 1.1 x 10^-3.

normalized_value_unit_evidence: Km: printed TABLE 2 cell 3100 uM; 3100 / 1000 = 3.1 mM. kcat: printed TABLE 2 cell 0.096 min^-1, under the column head 'kcat' whose unit line reads 'min' with the superscript -1; one min^-1 is 1/60 s^-1, so 0.096 / 60 = 0.0016 s^-1. kcat/Km: printed TABLE 2 cell 3.1 x 10^-5 min^-1 uM^-1, under the column head 'kcat/Km' whose unit line reads 'min' with the superscript -1 followed by 'uM' with the superscript -1; one min^-1 uM^-1 is 1000/60 s^-1 mM^-1, so 0.000031 x 1000 / 60 = 0.0005166666666666667 s^-1 mM^-1.

raw_value_unit_evidence: TABLE 2, Polymerase Dpo1, dNTP row T (dTTP): Km = 3100 uM; kcat = 0.096 min^-1; printed kcat/Km = 3.1 x 10^-5 min^-1 uM^-1.

row_label: TABLE 2, Polymerase block 'Dpo1', dNTP row 'T' (incoming nucleotide dTTP), insertion opposite template G


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature H242A to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 7; 298.15 K; cofactor ATP

proof_lines: mutant enzyme H242A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C

proof_lines: mutant enzyme H242A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C

proof_lines: mutant enzyme H242A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C


mutation_type: insertion

proof_lines: pH 7.5, 37°C, dTTP insertion opposite the N6dA-butanetriol in 5-TCTC-N6dA-butanetriol-GTTTATGGACCACC-3


proof_lines: pH 7.5, 37°C, dTTP insertion opposite the N6dA-(OH)2butyl-GSH in 5-TCTC-N6dA-(OH)2butyl-GSH-GTTTATGGACCACC-3


proof_lines: pH 7.5, 37°C, dTTP insertion opposite an unmodified deoxyadenosine in 5-TCTCAGTTTATGGACCACC-3


proof_lines: pH 7.5, 37°C, dCTP insertion opposite the N6dA-(OH)2butyl-GSH in 5-TCTC-N6dA-(OH)2butyl-GSH-GTTTATGGACCACC-3


proof_lines: pH 7.5, 37°C, dCTP insertion opposite the N6dA-butanetriol in 5-TCTC-N6dA-butanetriol-GTTTATGGACCACC-3


proof_lines: pH 7.5, 37°C, dCTP insertion opposite an unmodified deoxyadenosine in 5-TCTCAGTTTATGGACCACC-3


organism: Klebsiella aerogenes

assay_conditions_summary: pH 8; cofactor ATP

proof_lines: pH 8.0, 25, at a Mg2+/ATP ratio of 1.4/1

proof_lines: pH 8.0, 25, at a Mg2+/ATP ratio of 0.7/1


mutation_signature: PMCID:PMC2754150. The full-text sidecar places these Dpo4 rows in steady-state individual-dNTP insertion assays opposite MeFapy-dGuo lesions or unmodified dGuo

proof_lines: pH 7.8, 37°C, dATP insertion opposite N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine in 5-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimi

proof_lines: pH 7.8, 37°C, dATP insertion opposite N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine in 5-TCGT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimi


proof_lines: pH 7.8, 37°C, dCTP insertion opposite an unmodified deoxyguanosine in 5-TCAT-G-GAATCCTTACGAGCATCGCCCCC-3


proof_lines: pH 7.8, 37°C, dATP insertion opposite an unmodified deoxyguanosine in 5-TCAT-G-GAATCCTTACGAGCATCGCCCCC-3


proof_lines: pH 7.8, 37°C, dCTP insertion opposite N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine in 5-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimi

proof_lines: pH 7.8, 37°C, dCTP insertion opposite N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine in 5-TCGT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimi


proof_lines: pH 7.8, 37°C, dTTP insertion opposite N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine in 5-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimi


proof_lines: pH 7.8, 37°C, dCTP insertion opposite an unmodified deoxyguanosine in 5-TCGT-G-TCAATCCTTACGAGCATCGCCCCC-3


proof_lines: pH 7.8, 37°C, dGTP insertion opposite an unmodified deoxyguanosine in 5-TCAT-G-GAATCCTTACGAGCATCGCCCCC-3


proof_lines: pH 7.8, 37°C, dATP insertion opposite an unmodified deoxyguanosine in 5-TCGT-G-TCAATCCTTACGAGCATCGCCCCC-3


proof_lines: pH 7.8, 37°C, dTTP insertion opposite an unmodified deoxyguanosine in 5-TCAT-G-GAATCCTTACGAGCATCGCCCCC-3


proof_lines: pH 7.8, 37°C, dGTP insertion opposite N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine in 5-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimi


proof_lines: pH 7.8, 37°C, dGTP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylform


proof_lines: pH 7.8, 37°C, dGCTP insertion opposite an unmodified deoxyguanosine in 5-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3


proof_lines: pH 7.8, 37°C, dTTP insertion opposite an unmodified deoxyguanosine in 5-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3


proof_lines: pH 7.8, 37°C, dATP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylform

proof_lines: pH 7.8, 37°C, dATP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5-TCGT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylform


proof_lines: pH 7.8, 37°C, dTTP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylform


proof_lines: pH 7.8, 37°C, dCTP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5-TCGT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylform

proof_lines: pH 7.8, 37°C, dCTP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylform


proof_lines: pH 7.8, 37°C, dCTP insertion opposite an unmodified deoxyguanosine in 5-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3


proof_lines: pH 7.8, 37°C, dATP insertion opposite an unmodified deoxyguanosine in 5-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3


proof_lines: pH 7.8, 37°C, dGTP insertion opposite an unmodified deoxyguanosine in 5-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3


organism: Arabidopsis thaliana

substrate_name: dATP (with cytosine as template)

proof_lines: With cytosine as template, kcat is 0.024 min(-1) with dATP as substrate, kcat is 0.125 min(-1) with dGTP as substrate, kcat is 0.057 min(-1) with dATTP as substrate and kcat is 0.0038 min(-1) with dCTP as substrate. With thymine as template

substrate: dATP (with cytosine as template)


substrate_name: dGTP (with cytosine as template)

substrate: dGTP (with cytosine as template)


substrate_name: dTTP (with cytosine as template)

substrate: dTTP (with cytosine as template)


substrate_name: dCTP (with cytosine as template)

substrate: dCTP (with cytosine as template)


verification_status: verified

sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature T239W to reconstruct the assayed variant sequence.

proof_lines: PMID 18984592 Table 1: Dpo4 T239W, dCTP opposite template G, pH 7.4 at 37 C

status: verified


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature N188W to reconstruct the assayed variant sequence.

proof_lines: PMID 18984592 Table 1: Dpo4 N188W, dCTP opposite template G, pH 7.4 at 37 C


organism: Trypanosoma brucei

assay_conditions_summary: pH 6.7; 310.15 K; cofactor ATP

proof_lines: pH 6.7, 37°C, at 2.0 mM fructose 6-phosphate

proof_lines: pH 6.7, 37°C, at 0.4 mM fructose 6-phosphate

proof_lines: pH 6.7, 37°C, at 0.25 mM fructose 6-phosphate


substrate_name: dATP (for Pol-zeta2)

proof_lines: kcat is 0.31 min(-1) for DNA synthesis by Pol-zeta4. kcat is 0.05 min(-1) for DNA synthesis by Pol-zeta2.

substrate: dATP (for Pol-zeta2)


substrate_name: D-Fructose 6-phosphate; ATP

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

substrate: D-Fructose 6-phosphate; ATP


organism: Amycolatopsis methanolica

assay_conditions_summary: cofactor ATP


organism: Bacillus licheniformis

assay_conditions_summary: pH 6.8; 303.15 K; cofactor ATP


organism: Bacillus macquariensis


organism: Dictyostelium discoideum


substrate_name: ATP; D-Fructose 6-phosphate

substrate: ATP; D-Fructose 6-phosphate


organism: Thermus aquaticus

substrate_name: dADP

proof_lines: pH and temperature not specified in the publication

substrate: dADP


organism: Escherichia coli


organism: Geobacillus stearothermophilus


organism: Lactobacillus delbrueckii subsp. bulgaricus


organism: Pyrococcus furiosus

substrate_name: dATP

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature A408S to reconstruct the assayed variant sequence.

proof_lines: 55°C, pH not specified in the publication, mutant enzyme A408S

substrate: dATP


organism: Danio rerio

substrate_name: dTTP

proof_lines: pH 8.8, 30°C, recombinant enzyme, in presence of Mn2+

proof_lines: pH 8.8, 30°C, recombinant enzyme, in presence of Mg2+

substrate: dTTP


organism: Saccharolobus solfataricus

proof_lines: pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG

proof_lines: pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site

proof_lines: pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G


proof_lines: pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with O6-methylguanine as template

proof_lines: pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with guanine as template


proof_lines: pH 7.8, 37°C, incorporation of dCTP opposite (8oxoG) in the double stranded oligonucleotide: 5-GGGGGAAGGATTC-3/3-CCCCCTTCCTAAG(8oxoG)CACT-5


proof_lines: pH 7.5, 60°C, incorporation opposite the 3-thymine of an nondamaged template

proof_lines: pH 7.5, 60°C, incorporation opposite the 5-thymine of a cis-syn thymine dimer of the template

proof_lines: pH 7.5, 60°C, incorporation opposite the 3-thymine of a cis-syn thymine dimer of the template


organism: Lactobacillus plantarum


organism: Lactococcus lactis subsp. lactis


organism: Leishmania donovani


organism: Mus musculus


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)


organism: Oryctolagus cuniculus


organism: Rattus norvegicus


organism: Saccharomyces cerevisiae


organism: Thermus thermophilus

proof_lines: Full-text M1polWT dTTP kinetic extract: Km 230 +/- 70 uM, kcat 1.0 +/- 0.2 s-1; buffer B pH 8.2 at 50 C.


enzyme_display_name: hexokinase

organism: Bos taurus

smiles: C(C=O)C(C(C(CO)O)O)O

enzyme: hexokinase


enzyme_display_name: UniProt P19367

organism: Homo sapiens

substrate_name: D-glucose

enzyme: UniProt P19367

substrate: D-glucose


proof_lines:
Raw SKiD Env: Temp=25°C, pH=-----
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=30°C, pH=7.5


organism: Schistosoma mansoni


organism: Schizosaccharomyces pombe


organism: Spinacia oleracea


organism: Streptococcus thermophilus


organism: Streptomyces coelicolor


organism: Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)


organism: Thermococcus thioreducens

substrate_name: DNAn

substrate: DNAn


organism: Xenotropic MuLV-related virus (isolate VP62)

substrate_name: RNA/DNA duplex

substrate: RNA/DNA duplex


organism: Thermotoga maritima


organism: Trypanosoma brucei brucei


organism: Trypanosoma cruzi


organism: Ustilago maydis


organism: Bacillus subtilis


organism: Bacteriophage T4

substrate_name: Deoxynucleoside triphosphate; DNA

substrate: Deoxynucleoside triphosphate; DNA


organism: Enterobacteria phage PRD1

substrate_name: deoxynucleoside triphosphate

substrate: deoxynucleoside triphosphate


organism: Escherichia coli

substrate_name: H2O; DNA

substrate: H2O; DNA


organism: Homo sapiens

substrate_name: DNA; dATP

substrate: DNA; dATP


organism: Aspergillus niger

assay_conditions_summary: pH 7.8; cofactor ATP

proof_lines: pH 7.8, in presence of 5 mM Mg2+, native enzyme form


organism: Dictyostelium discoideum

proof_lines: SABIO-RK modifier species: 6-phosphofructokinase(Enzyme) mutant 4 N-terminal and 4 C-terminal subunits


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature R82E with inferred residue-numbering offset +8 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: 6-phosphofructokinase(Enzyme) mutant R82E


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature R77A with inferred residue-numbering offset +13 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: 6-phosphofructokinase(Enzyme) mutant R77A


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature R77D with inferred residue-numbering offset +13 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: 6-phosphofructokinase(Enzyme) mutant R77D


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature R77L with inferred residue-numbering offset +13 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: 6-phosphofructokinase(Enzyme) mutant R77L


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature R77E with inferred residue-numbering offset +13 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: 6-phosphofructokinase(Enzyme) mutant R77E


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature R82A with inferred residue-numbering offset +8 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: 6-phosphofructokinase(Enzyme) mutant R82A


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature Δ311 to reconstruct the assayed variant sequence.

mutation_type: deletion

proof_lines: SABIO-RK modifier species: 6-phosphofructokinase(Enzyme) wildtype delta311-319


proof_lines: SABIO-RK modifier species: 6-phosphofructokinase(Enzyme) mutant D127S


sequence_variant_note: Mutation signature T125S could not be applied safely because the resolved sequence does not match the expected reference residue(s).

proof_lines: SABIO-RK modifier species: 6-phosphofructokinase(Enzyme) mutant Thr125Ser


organism: Fasciola hepatica

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines:
pH 8.0, native PFK
pH 8.0, phosphorylated PFK


organism: Brugia malayi

substrate_name: D-Glucose; ATP

sequence_source: UniProtKB direct lookup of Q56VN6 on 2026-05-25; active primary accession A0A0K0JFP3 / HXK_BRUMA.

substrate: D-Glucose; ATP


substrate_name: ATP

substrate: ATP


substrate_name: D-fructose

substrate: D-fructose


organism: Mus musculus

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


organism: Rattus norvegicus


substrate_name: alpha-D-Glucose 6-phosphate; ADP

assay_conditions_summary: cofactor ADP

substrate: alpha-D-Glucose 6-phosphate; ADP


substrate_name: alpha-D-Glucose; ATP

substrate: alpha-D-Glucose; ATP


substrate_name: ATP; alpha-D-Glucose

substrate: ATP; alpha-D-Glucose


organism: Saccharomyces cerevisiae


substrate_name: D-Hexose; ATP

substrate: D-Hexose; ATP


organism: Bubalus bubalis


assay_conditions_summary: pH 8.1; 303.15 K; cofactor ATP


substrate_name: D-glucosamine

substrate: D-glucosamine


substrate_name: Unknown

proof_lines:
versus MgATP2-, at 30°C, pH 8.1
versus glucose, at 30°C, pH 8.1
at 30°C, pH 8.1

substrate: Unknown


organism: Mammalia

proof_lines:
type I isozyme
type III isozyme
type II isozyme


proof_lines:
type III isozyme
type II isozyme
type I isozyme


proof_lines:
type I and type II isozymes
type III isozyme


organism: Catharanthus roseus


substrate_name: UTP

substrate: UTP


organism: Aeropyrum pernix

substrate_name: D-mannose

assay_conditions_summary: pH 6.2; 363.15 K; cofactor ATP

substrate: D-mannose


organism: Brassica rapa subsp. oleifera

proof_lines:
isozyme HKI
isozyme HKII


substrate_name: D-glucose

substrate: D-glucose


organism: Lithobates sylvaticus

assay_conditions_summary: pH 8.2; 278.15 K; cofactor ATP

proof_lines:
control, pH 8.2, 5°C, no glucose
frozen, pH 8.2, 25°C
frozen, pH 8.2, 25°C, no glucose


proof_lines:
frozen, pH 8.2, 5°C, no glucose
control, pH 8.2, 25°C
control, pH 8.2, 25°C, no glucose


organism: Littorina littorea

proof_lines: enzyme from hepatopancreas, 24 h anoxic conditions, pH 7.5, 22°C

proof_lines: enzyme from hepatopancreas, pH 7.5, 22°C

proof_lines: enzyme from foot muscle, 24 h anoxic conditions, pH 7.5, 22°C


assay_conditions_summary: pH 7.5; 295.15 K; cofactor ATP


organism: Ogataea angusta

assay_conditions_summary: pH 7.5; cofactor ATP


organism: Thermus thermophilus


organism: Asterias amurensis


organism: Urocitellus undulatus

proof_lines: enzyme from hibernating animals, S0.5 value, Hill coefficient 1.2, pH 7.8, 2°C

proof_lines: enzyme from active animals, S0.5 value, Hill coefficient 1.16, pH 7.8, 2°C

proof_lines: enzyme from hibernating animals, S0.5 value, Hill coefficient 1.6, pH 7.8, 25°C


organism: Homo sapiens

mutation_signature: deletion mutant lacking residues 70-71

sequence_variant_note: Mutation context was captured, but the signature could not be translated into an exact residue substitution safely.

proof_lines: deletion mutant lacking residues 70-71, pH 7.6, 25 °C

proof_lines: deletion mutant lacking residues 70-71, K0.5 value, Hill coefficient 1.3, pH 7.6, 25 °C


mutation_signature: deletion mutant lacking residue 71

proof_lines: deletion mutant lacking residue 71, pH 7.6, 25 °C

proof_lines: deletion mutant lacking residue 71, K0.5 value, Hill coefficient 1.5, pH 7.6, 25 °C


mutation_signature: deletion mutant lacking residues 66-71

proof_lines: deletion mutant lacking residues 66-71, pH 7.6, 25 °C

proof_lines: deletion mutant lacking residues 66-71, K0.5 value, Hill coefficient 1.2, pH 7.6, 25 °C


mutation_signature: deletion mutant lacking residues 68-71

proof_lines: deletion mutant lacking residues 68-71, pH 7.6, 25 °C

proof_lines: deletion mutant lacking residues 68-71, K0.5 value, Hill coefficient 1.2, pH 7.6, 25 °C


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature S64R/E67D/S69T to reconstruct the assayed variant sequence.

proof_lines: mutant S64R/E67D/S69T, K0.5 value, Hill coefficient 1.7, pH 7.6, 25 °C


mutation_signature: deletion mutant lacking residues 67-71

proof_lines: deletion mutant lacking residues 67-71, pH 7.6, 25 °C

proof_lines: deletion mutant lacking residues 67-71, K0.5 value, Hill coefficient 1.1, pH 7.6, 25 °C


assay_conditions_summary: pH 7.6; 298.15 K; cofactor ATP


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature V62E to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature V62T to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature V62M with inferred residue-numbering offset +10 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature V62L to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature V62A with inferred residue-numbering offset +10 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature V62F with inferred residue-numbering offset +10 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature V62K with inferred residue-numbering offset +10 to reconstruct the assayed variant sequence.


organism: Sulfurisphaera tokodaii

assay_conditions_summary: pH 7.5; 323.15 K; cofactor ATP

proof_lines: pH 7.5, 50°C, D-glucose as phosphoryl acceptor


proof_lines:
pH 7.5, 50°C, ATP as phosphoryl donor
pH 7.5, 50°C
Raw SKiD Env: Temp=50°C, pH=7.5


substrate_name: N-acetyl-D-glucosamine

substrate: N-acetyl-D-glucosamine


substrate_name: CTP

substrate: CTP


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature Y214A to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 7.1; cofactor ATP

column_label: Table 1; row label: Y214A; column label: kcat (sec^-1); raw value: 117.0; raw unit: sec^-1; normalized value: 117.0; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 117.0; normalized unit: s^-1

raw_value_unit_evidence: raw value: 117 (123, 111); raw unit: sec^-1

table_label: Table 1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature C213R to reconstruct the assayed variant sequence.

column_label: Table 1; row label: C213R; column label: kcat (sec^-1); raw value: 50.0; raw unit: sec^-1; normalized value: 50.0; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 50.0; normalized unit: s^-1

raw_value_unit_evidence: raw value: 50.0; raw unit: sec^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature M197E to reconstruct the assayed variant sequence.

column_label: Table 1; row label: M197E; column label: kcat (sec^-1); raw value: 17.7; raw unit: sec^-1; normalized value: 17.7; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 17.7; normalized unit: s^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature K414E to reconstruct the assayed variant sequence.

column_label: Table 1; row label: K414E; column label: kcat (sec^-1); raw value: 19.9; raw unit: sec^-1; normalized value: 19.9; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 19.9; normalized unit: s^-1

raw_value_unit_evidence: raw value: 19.9; raw unit: sec^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature Y215A to reconstruct the assayed variant sequence.

column_label: Table 1; row label: Y215A; column label: kcat (sec^-1); raw value: 44.7; raw unit: sec^-1; normalized value: 44.7; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 44.7; normalized unit: s^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature K140E to reconstruct the assayed variant sequence.

column_label: Table 1; row label: K140E; column label: kcat (sec^-1); raw value: 40.0; raw unit: sec^-1; normalized value: 40.0; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 40.0; normalized unit: s^-1

raw_value_unit_evidence: raw value: 40.0±1.36; raw unit: sec^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature C252Y to reconstruct the assayed variant sequence.

column_label: Table 1; row label: C252Y; column label: kcat (sec^-1); raw value: 29.3; raw unit: sec^-1; normalized value: 29.3; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 29.3; normalized unit: s^-1

raw_value_unit_evidence: raw value: 29.3; raw unit: sec^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature V452L to reconstruct the assayed variant sequence.

column_label: Table 1; row label: V452L; column label: kcat (sec^-1); raw value: 122.0; raw unit: sec^-1; normalized value: 122.0; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 122.0; normalized unit: s^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature P417R to reconstruct the assayed variant sequence.

column_label: Table 1; row label: P417R; column label: kcat (sec^-1); raw value: 48.3; raw unit: sec^-1; normalized value: 48.3; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 48.3; normalized unit: s^-1

raw_value_unit_evidence: raw value: 48.3±7.40; raw unit: sec^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature T65I to reconstruct the assayed variant sequence.

column_label: Table 1; row label: T65I; column label: kcat (sec^-1); raw value: 22.6; raw unit: sec^-1; normalized value: 22.6; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 22.6; normalized unit: s^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature S263P to reconstruct the assayed variant sequence.

column_label: Table 1; row label: S263P; column label: kcat (sec^-1); raw value: 44.6; raw unit: sec^-1; normalized value: 44.6; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 44.6; normalized unit: s^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature S64Y to reconstruct the assayed variant sequence.

column_label: Table 1; row label: S64Y; column label: kcat (sec^-1); raw value: 114.0; raw unit: sec^-1; normalized value: 114.0; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 114.0; normalized unit: s^-1

raw_value_unit_evidence: raw value: 114 (111, 116); raw unit: sec^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature M298K to reconstruct the assayed variant sequence.

column_label: Table 1; row label: M298K; column label: kcat (sec^-1); raw value: 37.7; raw unit: sec^-1; normalized value: 37.7; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 37.7; normalized unit: s^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature S64P to reconstruct the assayed variant sequence.

column_label: Table 1; row label: S64P; column label: kcat (sec^-1); raw value: 83.3; raw unit: sec^-1; normalized value: 83.3; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 83.3; normalized unit: s^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature A456V to reconstruct the assayed variant sequence.

column_label: Table 1; row label: A456V; column label: kcat (sec^-1); raw value: 65.6; raw unit: sec^-1; normalized value: 65.6; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 65.6; normalized unit: s^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature G68V to reconstruct the assayed variant sequence.

column_label: Table 1; row label: G68V; column label: kcat (sec^-1); raw value: 62.7; raw unit: sec^-1; normalized value: 62.7; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 62.7; normalized unit: s^-1


sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

proof_lines: pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant 454-Ala


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature Y214A/V452A to reconstruct the assayed variant sequence.

column_label: Table 1; row label: Y214A/V452A; column label: kcat (sec^-1); raw value: 23.1; raw unit: sec^-1; normalized value: 23.1; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 23.1; normalized unit: s^-1

raw_value_unit_evidence: raw value: 23.1±2.38; raw unit: sec^-1


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature M197I/A379T to reconstruct the assayed variant sequence.

proof_lines: pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197I/A379T


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature G68K to reconstruct the assayed variant sequence.

column_label: Table 1; row label: G68K; column label: kcat (sec^-1); raw value: 43.2; raw unit: sec^-1; normalized value: 43.2; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 43.2; normalized unit: s^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature M197I to reconstruct the assayed variant sequence.

column_label: Table 1; row label: M197I; column label: kcat (sec^-1); raw value: 58.1; raw unit: sec^-1; normalized value: 58.1; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 58.1; normalized unit: s^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature V91L to reconstruct the assayed variant sequence.

column_label: Table 1; row label: V91L; column label: kcat (sec^-1); raw value: 60.6; raw unit: sec^-1; normalized value: 60.6; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 60.6; normalized unit: s^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature A379T to reconstruct the assayed variant sequence.

column_label: Table 1; row label: A379T; column label: kcat (sec^-1); raw value: 61.2; raw unit: sec^-1; normalized value: 61.2; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 61.2; normalized unit: s^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature S336L to reconstruct the assayed variant sequence.

column_label: Table 1; row label: S336L; column label: kcat (sec^-1); raw value: 2.46; raw unit: sec^-1; normalized value: 2.46; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 2.46; normalized unit: s^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature M197L to reconstruct the assayed variant sequence.

column_label: Table 1; row label: M197L; column label: kcat (sec^-1); raw value: 62.6; raw unit: sec^-1; normalized value: 62.6; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 62.6; normalized unit: s^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature Y214C to reconstruct the assayed variant sequence.

column_label: Table 1; row label: Y214C; column label: kcat (sec^-1); raw value: 65.3; raw unit: sec^-1; normalized value: 65.3; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 65.3; normalized unit: s^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature V389L to reconstruct the assayed variant sequence.

column_label: Table 1; row label: V389L; column label: kcat (sec^-1); raw value: 67.1; raw unit: sec^-1; normalized value: 67.1; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 67.1; normalized unit: s^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature E442K to reconstruct the assayed variant sequence.

column_label: Table 1; row label: E442K; column label: kcat (sec^-1); raw value: 52.6; raw unit: sec^-1; normalized value: 52.6; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 52.6; normalized unit: s^-1


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature V455M to reconstruct the assayed variant sequence.

column_label: Table 1; row label: V455M; column label: kcat (sec^-1); raw value: 61.8; raw unit: sec^-1; normalized value: 61.8; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol A uses 5 mM ATP with 11 D-glucose dilutions; 150 mM KCl

normalized_value_unit_evidence: normalized value: 61.8; normalized unit: s^-1


column_label: Table 1; row label: G68K; column label: ATP Km (mM); raw value: 0.39; raw unit: mM; normalized value: 0.39; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 0.39; normalized unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature S263P to reconstruct the assayed variant sequence.

column_label: Table 1; row label: S263P; column label: ATP Km (mM); raw value: 0.63; raw unit: mM; normalized value: 0.63; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 0.63; normalized unit: mM


sequence_variant_note: Mutation signature V62M matches the mutant residue at offset -5 and the reference residue at offset +0; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

assay_conditions_summary: pH 7.1; 303.15 K; cofactor ATP

proof_lines: pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V62M

proof_lines: mutant enzyme V62M, at 30°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol

proof_lines: mutant enzyme V62M, at 37°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature M197E to reconstruct the assayed variant sequence.

column_label: Table 1; row label: M197E; column label: ATP Km (mM); raw value: 0.4; raw unit: mM; normalized value: 0.4; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 0.4; normalized unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature G68V to reconstruct the assayed variant sequence.

column_label: Table 1; row label: G68V; column label: ATP Km (mM); raw value: 0.31; raw unit: mM; normalized value: 0.31; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 0.31; normalized unit: mM


column_label: Table 1; row label: A379T; column label: ATP Km (mM); raw value: 0.87; raw unit: mM; normalized value: 0.87; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 0.87; normalized unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature S64P to reconstruct the assayed variant sequence.

column_label: Table 1; row label: S64P; column label: ATP Km (mM); raw value: 0.32; raw unit: mM; normalized value: 0.32; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 0.32; normalized unit: mM


column_label: Table 1; row label: E442K; column label: ATP Km (mM); raw value: 1.5; raw unit: mM; normalized value: 1.5; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 1.5; normalized unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature K140E to reconstruct the assayed variant sequence.

column_label: Table 1; row label: K140E; column label: ATP Km (mM); raw value: 0.35; raw unit: mM; normalized value: 0.35; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 0.35; normalized unit: mM

raw_value_unit_evidence: raw value: 0.35±0.08; raw unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature C213R to reconstruct the assayed variant sequence.

column_label: Table 1; row label: C213R; column label: ATP Km (mM); raw value: 0.89; raw unit: mM; normalized value: 0.89; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 0.89; normalized unit: mM

raw_value_unit_evidence: raw value: 0.89; raw unit: mM


column_label: Table 1; row label: V455M; column label: ATP Km (mM); raw value: 0.38; raw unit: mM; normalized value: 0.38; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 0.38; normalized unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature Y215A to reconstruct the assayed variant sequence.

column_label: Table 1; row label: Y215A; column label: ATP Km (mM); raw value: 0.58; raw unit: mM; normalized value: 0.58; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 0.58; normalized unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature S64Y to reconstruct the assayed variant sequence.

column_label: Table 1; row label: S64Y; column label: ATP Km (mM); raw value: 1.59; raw unit: mM; normalized value: 1.59; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 1.59; normalized unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature C252Y to reconstruct the assayed variant sequence.

column_label: Table 1; row label: C252Y; column label: ATP Km (mM); raw value: 0.75; raw unit: mM; normalized value: 0.75; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 0.75; normalized unit: mM

raw_value_unit_evidence: raw value: 0.75; raw unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature V452L to reconstruct the assayed variant sequence.

column_label: Table 1; row label: V452L; column label: ATP Km (mM); raw value: 0.53; raw unit: mM; normalized value: 0.53; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 0.53; normalized unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature V91L to reconstruct the assayed variant sequence.

column_label: Table 1; row label: V91L; column label: ATP Km (mM); raw value: 0.48; raw unit: mM; normalized value: 0.48; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 0.48; normalized unit: mM


column_label: Table 1; row label: V389L; column label: ATP Km (mM); raw value: 0.76; raw unit: mM; normalized value: 0.76; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 0.76; normalized unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature T65I to reconstruct the assayed variant sequence.

column_label: Table 1; row label: T65I; column label: ATP Km (mM); raw value: 0.57; raw unit: mM; normalized value: 0.57; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 0.57; normalized unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature Y214A to reconstruct the assayed variant sequence.

column_label: Table 1; row label: Y214A; column label: ATP Km (mM); raw value: 0.92; raw unit: mM; normalized value: 0.92; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 0.92; normalized unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature Y214C to reconstruct the assayed variant sequence.

column_label: Table 1; row label: Y214C; column label: ATP Km (mM); raw value: 1.08; raw unit: mM; normalized value: 1.08; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 1.08; normalized unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature S336L to reconstruct the assayed variant sequence.

column_label: Table 1; row label: S336L; column label: ATP Km (mM); raw value: 12.6; raw unit: mM; normalized value: 12.6; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 12.6; normalized unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature M197I to reconstruct the assayed variant sequence.

column_label: Table 1; row label: M197I; column label: ATP Km (mM); raw value: 1.39; raw unit: mM; normalized value: 1.39; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 1.39; normalized unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature Y214A/V452A to reconstruct the assayed variant sequence.

column_label: Table 1; row label: Y214A/V452A; column label: ATP Km (mM); raw value: 1.42; raw unit: mM; normalized value: 1.42; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 1.42; normalized unit: mM

raw_value_unit_evidence: raw value: 1.42±0.29; raw unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature P417R to reconstruct the assayed variant sequence.

column_label: Table 1; row label: P417R; column label: ATP Km (mM); raw value: 2.08; raw unit: mM; normalized value: 2.08; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 2.08; normalized unit: mM

raw_value_unit_evidence: raw value: 2.08±0.15; raw unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature K414E to reconstruct the assayed variant sequence.

column_label: Table 1; row label: K414E; column label: ATP Km (mM); raw value: 1.53; raw unit: mM; normalized value: 1.53; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 1.53; normalized unit: mM

raw_value_unit_evidence: raw value: 1.53; raw unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature M197L to reconstruct the assayed variant sequence.

column_label: Table 1; row label: M197L; column label: ATP Km (mM); raw value: 1.31; raw unit: mM; normalized value: 1.31; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 1.31; normalized unit: mM


sequence_variant_note: Resolved canonical sequence from official_uniprot_json: and applied mutation signature M298K to reconstruct the assayed variant sequence.

column_label: Table 1; row label: M298K; column label: ATP Km (mM); raw value: 3.32; raw unit: mM; normalized value: 3.32; assay condition: cleaved recombinant human glucokinase; routine NADP+ coupled assay; protocol B varies ATP at D-glucose 10x S0.5 with 1 mM MgCl2 excess; 150 mM KCl

normalized_value_unit_evidence: normalized value: 3.32; normalized unit: mM


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature M197I/A379T to reconstruct the assayed variant sequence.


organism: Sus scrofa

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines:
hexokinase II, at 30°C, pH 8.1
hexokinase III, at 30°C, pH 8.1
hexokinase IV


substrate_name: ITP

substrate: ITP


substrate_name: beta-D-glucose

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature W99R/W167F to reconstruct the assayed variant sequence.

proof_lines: co-substrate: ATP, mutant W99R/W167F in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication

proof_lines: co-substrate: ATP, mutant W99R/W167F in the presence of 20-30% glycerol and in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication

proof_lines: co-substrate: ATP, mutant W99R/W167F, pH and temperature not specified in the publication

substrate: beta-D-glucose


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature W99R/W257F to reconstruct the assayed variant sequence.

proof_lines: co-substrate: ATP, mutant W99R/W257F in the presence of 20-30% glycerol, pH and temperature not specified in the publication

proof_lines: co-substrate: ATP, mutant W99R/W257F in the presence of 20-30% glycerol and in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication

proof_lines: co-substrate: ATP, mutant W99R/W257F, pH and temperature not specified in the publication


proof_lines: mutant W99R/W257F in the presence of 20-30% glycerol, pH and temperature not specified in the publication

proof_lines: mutant W99R/W257F, pH and temperature not specified in the publication

proof_lines: mutant W99R/W257F in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication


proof_lines: mutant W99R/W167F in the presence of 20-30% glycerol, pH and temperature not specified in the publication

proof_lines: mutant W99R/W167F in the presence of 20-30% glycerol and in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication

proof_lines: mutant W99R/W167F, pH and temperature not specified in the publication


organism: Eimeria tenella


substrate_name: D-mannose; D-glucose

substrate: D-mannose; D-glucose


sequence_variant_note: Mutation signature N216K matches the mutant residue at offset +13 and the reference residue at offset +0; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature D217H to reconstruct the assayed variant sequence.


proof_lines:
Raw SKiD Env: Temp=30°C, pH=7.4
Raw SKiD Env: Temp=37°C, pH=7.4
Raw SKiD Env: Temp=-----, pH=7.1

source_databases_merged: skid uniprot


assay_conditions_summary: pH 7; 310.15 K; cofactor ATP

proof_lines:
Raw SKiD Env: Temp=°C, pH=7.6
Raw SKiD Env: Temp=37°C, pH=7.4
Raw SKiD Env: Temp=30°C, pH=7.4


substrate_name: 2-deoxyglucose (at 30 degrees Celsius and pH 7.5)

proof_lines: kcat is 66.4 sec(-1) with glucose as substrate (at 30 degrees Celsius and pH 7.5) (PubMed:7742312). kcat is 56.4 sec(-1) with mannose as substrate (at 30 degrees Celsius and pH 7.5) (PubMed:7742312). kcat is 56.4 sec(-1) with 2-deoxyglucose


substrate_name: fructose (at 30 degrees Celsius and pH 7.5)


substrate_name: glucosamine (at 30 degrees Celsius and pH 9)

assay_conditions_summary: pH 9


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature G72R to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

proof_lines: mutant enzyme G72R, at 37°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol

proof_lines: pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant G72R

proof_lines: mutant enzyme G72R, at 30°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol


sequence_variant_note: Mutation signature V62M matches the mutant residue at offset -5 and the reference residue at offset +0; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

assay_conditions_summary: pH 7.4; 303.15 K; cofactor ATP

proof_lines: mutant enzyme V62M, at 30°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol

proof_lines: mutant enzyme V62M, at 37°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol

proof_lines: pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V62M


proof_lines: wild type enzyme, at 30°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol

proof_lines: wild type enzyme, at 37°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol


substrate_name: ATP

proof_lines: pH 7.1, temperature not specified in the publication, co-substrate: ATP, wild-type


sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


organism: Mus musculus

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature A456V to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature K414E to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature S151G to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature S151C/N166R to reconstruct the assayed variant sequence.


substrate_name: D-fructose


sequence_variant_note: Resolved canonical sequence from selected_batch_variant_sequence_check and applied mutation signature V182L to reconstruct the assayed variant sequence.

assay_conditions_summary: cofactor ATP

proof_lines: mutant enzyme V182L, glutathione S-transferase glucokinase B fusion protein


sequence_variant_note: Resolved canonical sequence from selected_batch_variant_sequence_check and applied mutation signature K420E to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme K420E, glutathione S-transferase glucokinase B fusion protein


sequence_variant_note: Resolved canonical sequence from selected_batch_variant_sequence_check and applied mutation signature E265K to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme E265K, glutathione S-transferase glucokinase B fusion protein


sequence_variant_note: Resolved canonical sequence from selected_batch_variant_sequence_check and applied mutation signature Y61S to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme Y61S, glutathione S-transferase glucokinase B fusion protein


sequence_variant_note: Resolved canonical sequence from selected_batch_variant_sequence_check and applied mutation signature A379V to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme A379V, glutathione S-transferase glucokinase B fusion protein


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature W167F to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme W167F, in 25 mM HEPES (pH 7.4), at 37°C


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature W99F to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme W99F, in 25 mM HEPES (pH 7.4), at 37°C


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature W257F to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme W257F, in 25 mM HEPES (pH 7.4), at 37°C


organism: Cryptosporidium parvum

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: pH 7.5, temperature not specified in the publication


organism: Nicotiana tabacum

proof_lines: pH 8.4, 30°C, recombinant isozyme hexokinase 2


organism: Trypanosoma brucei


assay_conditions_summary: pH 7.9; cofactor ATP


organism: Arabidopsis thaliana

substrate_name: glucose

proof_lines: Measured in yeast lacking glucose and hexose kinase activity.

substrate: glucose


substrate_name: fructose

substrate: fructose


organism: Saccharomyces cerevisiae

proof_lines:
S-nitrosoglutathione treated enzyme
Raw SKiD Env: Temp=-----, pH=-----


substrate: D-fructose


organism: Bos taurus

proof_lines: pH 7.2, 37°C, recombinant catalytic C-terminal domain

proof_lines: pH 7.2, 37°C, recombinant full length enzyme


assay_conditions_summary: pH 7.2; 310.15 K; cofactor ATP

substrate: ATP


organism: Kluyveromyces lactis

assay_conditions_summary: pH 7.4; 298.15 K; cofactor ATP

proof_lines: pH 7.4, 25°C, recombinant enzyme at 1.0 mg/ml

proof_lines: pH 7.4, 25°C, recombinant enzyme at 0.001 mg/ml


organism: Rattus norvegicus

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature D84A with inferred residue-numbering offset +6 to reconstruct the assayed variant sequence.

proof_lines:
HK I and its D84A mutant
HK I+ and its D84A mutant


proof_lines: recombinant wild-type hexokinase I, D84A, D84E and D84K mutants


proof_lines: pH and temperature not specified in the publication


proof_lines: wild-type and nonaggregating interface mutant hexokinase I, pH 7.8


organism: Ascaris suum

substrate_name: Glucose; ATP

substrate: Glucose; ATP


organism: Aspergillus niger


organism: Bacteroides fragilis

substrate_name: D-Glucose; ATP

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

substrate: D-Glucose; ATP


organism: Botrytis cinerea

substrate_name: ATP; D-Glucose

substrate: ATP; D-Glucose


organism: Candida albicans


organism: Solanum tuberosum

substrate_name: glucose; mannose

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

substrate: glucose; mannose


organism: Xenopus laevis

substrate_name: D-glucose

assay_conditions_summary: pH 8; 295.15 K; cofactor ATP

proof_lines: enzyme isolated from dehydrated frogs, pH 8.0, 22°C


organism: Schwanniomyces occidentalis


organism: Homo sapiens

assay_conditions_summary: pH 7.1; 298.15 K; cofactor ATP

proof_lines: wild type enzyme, in 25 mM HEPES (pH 7.1), at 25°C


substrate_name: dTTP (in presence of 2 mM of Mn(2+))

proof_lines: kcat is 0.05 sec(-1) for dTTP (in presence of 2 mM of Mn(2+)). kcat is 0.02 sec(-1) for dTTP (in presence of 50 uM of Mn(2+)). kcat is 0.01 sec(-1) for dTTP (in presence of 2 mM of Mg(2+)).

substrate: dTTP (in presence of 2 mM of Mn(2+))


organism: Plasmodium falciparum


organism: Entamoeba histolytica


assay_conditions_summary: pH 8.5; 310.15 K; cofactor ATP


sequence_variant_note: Resolved canonical sequence from multiple and applied mutation signature A456V to reconstruct the assayed variant sequence.

proof_lines: pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant A456V


organism: Trypanosoma cruzi

proof_lines: natural hexokinase from epimastigotes, at pH 7.5

proof_lines: recombinant hexokinase expressed in Escherichia coli XL-1 Blue, at pH 7.5


assay_conditions_summary: pH 7.5; cofactor ATP

proof_lines: natural and recombinant hexokinase, expressed in Escherichia coli XL-1 Blue, at pH 7.5


organism: Leishmania mexicana

substrate_name: ATP; Glucose

substrate: ATP; Glucose


organism: Macrobrachium nipponense

substrate_name: Unknown

substrate: Unknown


organism: Mus musculus


organism: Nicotiana tabacum


organism: Ogataea angusta


organism: Rattus norvegicus


organism: Saccharomyces cerevisiae


organism: Schistosoma mansoni

assay_conditions_summary: pH 8.5; cofactor ATP


organism: Sulfolobus tokodaii

substrate_name: D-Mannose; ATP

assay_conditions_summary: pH 7.5; 323.15 K; cofactor ATP

substrate: D-Mannose; ATP


organism: Oncorhynchus mykiss


organism: Pisum sativum


substrate_name: D-Glucose; ITP

substrate: D-Glucose; ITP


organism: Sus scrofa


organism: Thermoproteus tenax


organism: Toxoplasma gondii


organism: Triticum aestivum

substrate_name: Fructose; ATP

substrate: Fructose; ATP


organism: Trypanosoma brucei


organism: Entamoeba histolytica

sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature V183T to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant HXK1 V183T


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature V183T/I270S to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant HXK1 V183T/I270S


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature S144T/V183T/I270S to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant HXK1 S144T/V183T/I270S


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature S144T/R265T/Y266F to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant HXK1 S144T/R265T/Y266F


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature S144T/V183T/I270S/R265T/Y266F to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant HXK1 S144T/V183T/I270S/R265T/Y266F


organism: Homo sapiens

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.

proof_lines: nonaggregating interface mutant hexokinase I, pH 7.8


organism: Bos taurus

proof_lines: pH 7.2, 37°C, recombinant full length enzyme, versus MgATP2-

proof_lines: pH 7.2, 37°C, recombinant catalytic C-terminal domain, versus MgATP2-


organism: Cryptosporidium parvum

proof_lines: pH 7.5, temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature V183T/I270S/R265T/Y266F to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant HXK1 V183T/I270S/R265T/Y266F


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature I270S to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant HXK1 I270S


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature R265T/Y266F to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant HXK1 R265T/Y266F


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature S144T to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant HXK1 S144T


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature A464P/E465G to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant isozyme HK1 A464P/E465G


sequence_variant_note: Mutation context was captured, but the signature could not be translated into an exact residue substitution safely.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant isozyme HK1 Gln466 7 AA insertion His467


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature Δ16 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) wildtype delta16 (aa 17-916)


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature D209A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant D209A (aa 17-916)


sequence_variant_note: Mutation signature D657A matches the mutant residue at offset -4 and the reference residue at offset +0; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant D657A (aa 17-916)


proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant N166R


sequence_variant_note: Resolved canonical sequence from selected_batch_sequence_residue_checked_against_mutation_signature and applied mutation signature S151A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant S151A


sequence_variant_note: Resolved canonical sequence from selected_batch_sequence_residue_checked_against_mutation_signature and applied mutation signature S151G to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant S151G


sequence_variant_note: Resolved canonical sequence from selected_batch_sequence_residue_checked_against_mutation_signature and applied mutation signature S151C to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant S151C


proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant N166R-S151A


organism: Mus musculus

sequence_variant_note: Resolved canonical sequence from uniprot_p17710_direct_reconstruction and applied mutation signature S603A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant S603A


sequence_variant_note: Mutation signature K558R could not be applied safely because the resolved sequence does not match the expected reference residue(s).

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant K558R


sequence_variant_note: Mutation signature K558M could not be applied safely because the resolved sequence does not match the expected reference residue(s).

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant K558M


sequence_variant_note: Mutation signature E708A could not be applied safely because the resolved sequence does not match the expected reference residue(s).

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant E708A


sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset -2, so it is treated as a source-provided variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant E742A


sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant C-terminal half


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature D84A with inferred residue-numbering offset +6 to reconstruct the assayed variant sequence.

proof_lines:
HK I+ and its D84A mutant
D84A mutant of HK I


sequence_variant_note: Mutation signature D84A matches the mutant residue at offset -2 and the reference residue at offset +0; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant isozyme Type I D84A


sequence_variant_note: Mutation signature D84K matches the mutant residue at offset -7 and the reference residue at offset +0; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant isozyme Type I D84K


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


sequence_variant_note: Mutation signature D84E matches the mutant residue at offset -8 and the reference residue at offset +0; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant isozyme Type I D84E


organism: Schizosaccharomyces pombe

substrate_name: ATP; D-Mannose

substrate: ATP; D-Mannose


substrate_name: ATP; D-Fructose

substrate: ATP; D-Fructose


organism: Solanum chacoense


organism: Solanum tuberosum

proof_lines: Measured in yeast lacking glucose and hexose kinase activity.

source_databases_merged: sabio_rk brenda uniprot


proof_lines: uncomplexed ATP4-, hexokinase III, at 30°C, pH 7.2

proof_lines: uncomplexed, hexokinase III, at 30°C, pH 7.2


proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant HK2C1 (C-terminus of HK1)


organism: Trypanosoma cruzi

proof_lines: natural hexokinase from epimastigotes, at pH 7.5

proof_lines: recombinant hexokinase expressed in Escherichia coli, at pH 7.5


sequence_variant_note: Mutation signature E260A could not be applied safely because the resolved sequence does not match the expected reference residue(s).

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant E260A


sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset +13, so it is treated as a source-provided variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant D209A


sequence_variant_note: Mutation signature S155A could not be applied safely because the resolved sequence does not match the expected reference residue(s).

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant S155A


organism: Rattus norvegicus

sequence_variant_note: Resolved canonical sequence from reconstructed_variant_sequence and applied mutation signature S603A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant S603A (N-terminal half of type II isozyme)


sequence_variant_note: Resolved canonical sequence from reconstructed_variant_sequence and applied mutation signature D447S/S603A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant D447S/S603A (N-terminal half of type II isozyme)


sequence_variant_note: Mutation signature G174R/S603A matches the mutant residue at offset +0 and the reference residue at offset +23; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: hexokinase(Enzyme) mutant G174R/S603A (N-terminal half of type II isozyme)


organism: Gallus gallus

substrate_name: D-Fructose 6-phosphate; ATP

substrate: D-Fructose 6-phosphate; ATP


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature H242A to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 7; 298.15 K; cofactor ATP

proof_lines: Ki above 10 mM, mutant enzyme H242A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C

proof_lines: Ki above 10 mM, mutant enzyme H242A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C

proof_lines: mutant enzyme H242A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C


proof_lines: SABIO-RK modifier species: 6-phosphofructokinase(Enzyme) mutant 4 N-terminal and 4 C-terminal subunits


sequence_variant_note: Mutation context was captured, but the signature could not be translated into an exact residue substitution safely.

proof_lines: N-terminal truncation mutant, pH 7.1, 25°C


organism: Lactobacillus acidophilus


organism: Bacteriophage RB69

substrate_name: DNA; dATP

sequence_variant_note: Resolved canonical sequence from uniprot_accession plus direct reconstruction from Q38087 canonical sequence and applied mutation signature D222A/D327A/N564A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/N564A

substrate: DNA; dATP


substrate_name: dGDP

sequence_variant_note: Resolved canonical sequence from uniprot_accession plus direct reconstruction from Q38087 canonical sequence and applied mutation signature D222A/D327A/K560A to reconstruct the assayed variant sequence.

substrate: dGDP


sequence_variant_note: Resolved canonical sequence from uniprot_accession plus direct reconstruction from Q38087 canonical sequence and applied mutation signature D222A/D327A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A


sequence_variant_note: Resolved canonical sequence from uniprot_accession plus direct reconstruction from Q38087 canonical sequence and applied mutation signature D222A/D327A/K483A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K483A


substrate_name: dATP; DNA

sequence_variant_note: Resolved canonical sequence from uniprot_accession plus direct reconstruction from Q38087 canonical sequence and applied mutation signature D222A/D327A/R482A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/R482A

substrate: dATP; DNA


sequence_source: uniprot_accession plus direct reconstruction from Q38087 canonical sequence

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K560A


organism: Lactococcus lactis subsp. lactis

substrate_name: ATP; D-Fructose 6-phosphate

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

proof_lines: SABIO-RK modifier species: 6-phosphofructokinase(Enzyme) mutant Lac- Gal d

substrate: ATP; D-Fructose 6-phosphate


substrate_name: Fructose 6-phosphate; ATP

sequence_variant_note: Resolved canonical sequence from reconstructed_variant_sequence and applied mutation signature Y139F to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: 6-phosphofructokinase(Enzyme) mutant Tyr139Phe His6-tagged

substrate: Fructose 6-phosphate; ATP


substrate_name: ATP; Fructose 6-phosphate

sequence_variant_note: Resolved canonical sequence from reconstructed_variant_sequence and applied mutation signature K226G to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: 6-phosphofructokinase(Enzyme) mutant Lys226Gly His6-tagged

substrate: ATP; Fructose 6-phosphate


organism: [Bacillus] caldotenax

substrate_name: deoxynucleoside triphosphate

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: equimolar mixture of all nucleotides, pH 7.5, 70°C

substrate: deoxynucleoside triphosphate


organism: Bacillus subtilis

substrate_name: dGTP; DNA

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant drug resistant

substrate: dGTP; DNA


sequence_variant_note: Resolved canonical sequence from reconstructed_variant_sequence and applied mutation signature D222A/D327A/Y567A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/Y567A


sequence_variant_note: Resolved canonical sequence from selected_batch_reconstructed_from_uniprot_Q38087 and applied mutation signature D222A/D327A/K486A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant D222A/D327A/K486A


substrate_name: DNA; dGTP

sequence_variant_note: Mutation signature S624C matches the mutant residue at offset -15 and the reference residue at offset +0; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant S624C

substrate: DNA; dGTP


sequence_variant_note: Resolved canonical sequence from selected_batch_reconstructed_from_uniprot_Q38087 and applied mutation signature D621A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant D621A


sequence_variant_note: Resolved canonical sequence from selected_batch_reconstructed_from_uniprot_Q38087 and applied mutation signature Y708A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant Y708A


sequence_variant_note: Resolved canonical sequence from selected_batch_reconstructed_from_uniprot_Q38087 and applied mutation signature E686A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant E686A


substrate_name: dCTP; DNA

sequence_variant_note: Resolved canonical sequence from reconstructed_from_uniprot_accession_Q38087 and applied mutation signature T587A/Y391A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant T587A/Y391A

substrate: dCTP; DNA


organism: Bacteriophage T4

substrate_name: DNA; H2O

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant D112N

substrate: DNA; H2O


substrate_name: H2O; DNA

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant D324A

substrate: H2O; DNA


sequence_variant_note: Resolved canonical sequence from reconstructed_from_uniprot_accession_P04415 and applied mutation signature E114A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant E114A


sequence_variant_note: Resolved canonical sequence from reconstructed_from_uniprot_accession_P04415 and applied mutation signature D112A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant D112A


proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant D219A


sequence_variant_note: Resolved canonical sequence from UniProt Q38087 direct FASTA check plus local substitution reconstruction and applied mutation signature Y391F to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant Y391F


substrate_name: DNA; dCTP

sequence_variant_note: Resolved canonical sequence from UniProt Q38087 direct FASTA check plus local substitution reconstruction and applied mutation signature T587A/Y391F to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant T587A/Y391F

substrate: DNA; dCTP


sequence_variant_note: Resolved canonical sequence from UniProt Q38087 direct FASTA check plus local substitution reconstruction and applied mutation signature Y567F to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant Y567F


sequence_variant_note: Resolved canonical sequence from UniProt Q38087 direct FASTA check plus local substitution reconstruction and applied mutation signature Y391A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant Y391A


sequence_variant_note: Resolved canonical sequence from UniProt Q38087 direct FASTA check plus local substitution reconstruction and applied mutation signature T587A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: DNA-directed DNA polymerase(Enzyme) mutant T587A