enzyme_display_name: glycine oxidase

organism: Geobacillus kaustophilus

substrate_name: N-ethylglycine

smiles: CCNCC(=O)O

assay_conditions_summary: pH 8.5; 310.15 K; cofactor FAD

proof_lines: in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C

enzyme: glycine oxidase

substrate: N-ethylglycine


substrate_name: glycine

source_db: brenda

verification_status: unverified

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

smiles: C(C(=O)O)N

substrate: glycine

status: unverified

source: brenda


substrate_name: sarcosine

smiles: CNCC(=O)O

substrate: sarcosine


substrate_name: glycine-ethyl-ester

smiles: CCOC(=O)CN

substrate: glycine-ethyl-ester


substrate_name: D-proline

substrate: D-proline


substrate_name: D-alanine

smiles: CC(C(=O)O)N

substrate: D-alanine


substrate_name: D-pipecolate

substrate: D-pipecolate


enzyme_display_name: primary-amine oxidase

organism: Trifolium pratense

substrate_name: putrescine

smiles: C(CCN)CN

enzyme: primary-amine oxidase

substrate: putrescine


substrate_name: cadaverine

smiles: C(CCN)CCN

substrate: cadaverine


smiles: C(CCCCN)CCCN


substrate_name: spermidine

smiles: C(CCNCCCN)CN

substrate: spermidine


substrate_name: spermine

smiles: C(CCNCCCN)CNCCCN

substrate: spermine


substrate_name: tyramine

substrate: tyramine


substrate_name: benzylamine

substrate: benzylamine


substrate_name: Phenylethylamine

substrate: Phenylethylamine


enzyme_display_name: diamine oxidase

organism: Bos taurus

smiles: C(CN)CN

enzyme: diamine oxidase


smiles: CCCCCCCCCN


smiles: CC(=O)NCCCCNCCCN


proof_lines:
pH 7.2, 37°C
immobilized enzyme
free enzyme


smiles: CCCCN


proof_lines:
pH 7.2, 37°C
free enzyme
immobilized enzyme


substrate_name: benzaldehyde

substrate: benzaldehyde


substrate_name: Propylamine

smiles: CCCN

proof_lines:
immobilized enzyme
free enzyme

substrate: Propylamine


organism: Rattus norvegicus

substrate_name: histamine

proof_lines: in 100 mM potassium phosphate, pH 7.2, at 37°C

proof_lines: pH and temperature not specified in the publication

substrate: histamine


enzyme_display_name: D-amino-acid oxidase

substrate_name: D-tryptophan

assay_conditions_summary: pH 8.3; 298.15 K; buffer 75 mM sodium diphosphate

proof_lines: in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C

enzyme: D-amino-acid oxidase

substrate: D-tryptophan


substrate_name: D-serine

smiles: C(C(C(=O)O)N)O

substrate: D-serine


substrate_name: D-phenylalanine

substrate: D-phenylalanine


smiles: O=O


substrate_name: Unknown

km_display: Not reported

ki_unit: mM

substrate: Unknown


organism: Rhodotorula toruloides

substrate_name: D-Asp

proof_lines: wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value

substrate: D-Asp


substrate_name: D-Glu

smiles: C(CC(=O)O)C(C(=O)O)N

proof_lines: wild type enzyme, apparent value, at 25°C and pH 8.5

substrate: D-Glu


substrate_name: N-Methyl-D-aspartate

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

smiles: CNC(CC(=O)O)C(=O)O

mutation_type: substitution

substrate: N-Methyl-D-aspartate


substrate_name: D-Asn

substrate: D-Asn


proof_lines: mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value


substrate_name: D-Ala

substrate: D-Ala


substrate_name: D-Gln

smiles: C(CC(=O)N)C(C(=O)O)N

proof_lines: mutant enzyme M213R, apparent value, at 25°C and pH 8.5

substrate: D-Gln


substrate_name: D-Met

smiles: CSCCC(C(=O)O)N

substrate: D-Met


substrate_name: D-Pro

substrate: D-Pro


proof_lines:
pH 8.5, 25°C, wild-type enzyme
immobilized wild type enzyme, at 25°C
free wild type enzyme, at 25°C


proof_lines:
with D-methionine
at pH 8.3 and 25°C


substrate_name: D-norleucine

smiles: CCCCC(C(=O)O)N

substrate: D-norleucine


substrate_name: D-leucine

smiles: CC(C)CC(C(=O)O)N

substrate: D-leucine


substrate_name: D-methionine

substrate: D-methionine


substrate_name: D-Norvaline

smiles: CCCC(C(=O)O)N

substrate: D-Norvaline


substrate_name: D-cysteine

smiles: C(C(C(=O)O)N)S

substrate: D-cysteine


assay_conditions_summary: pH 8.5; 298.15 K; buffer 75 mM sodium diphosphate

proof_lines: wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C


proof_lines: mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C


substrate_name: D-phenylglycine

substrate: D-phenylglycine


substrate_name: D-alanine

smiles: CC(C(=O)O)N

substrate: D-alanine


substrate_name: D-Trp

proof_lines: pH 7.5, 25°C, mutant enzyme DELTASer308-Lys321

substrate: D-Trp


assay_conditions_summary: cofactor FAD

proof_lines: mutant enzyme W243I, assay in presence of a large excess of free FAD (0.2 mM) in assay mixture


substrate_name: D-Lysine

smiles: C(CCN)CC(C(=O)O)N

substrate: D-Lysine


substrate_name: D-glutamate

substrate: D-glutamate


substrate_name: D-proline

substrate: D-proline


substrate_name: D-valine

smiles: CC(C)C(C(=O)O)N

substrate: D-valine


proof_lines: pH 8.5, 25°C, mutant enzyme S335G, reaction with D-Ala


proof_lines: pH 8.5, 25°C, wild-type enzyme, reaction with D-Ala


assay_conditions_summary: pH 8; 298.15 K; buffer 100 mM potassium phosphate

proof_lines: immobilized enzyme, in 100 mM potassium phosphate buffer at pH 8.0 and 25°C


substrate_name: Unknown

substrate: Unknown


substrate_name: cephalosporin C

proof_lines: immobilized enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25°C

proof_lines: free enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25°C

substrate: cephalosporin C


substrate_name: D-Ser

proof_lines:
apparent value, immobilized enzyme
apparent value, free enzyme

substrate: D-Ser


organism: Rubrobacter xylanophilus

substrate_name: D-Thr

smiles: CC(C(C(=O)O)N)O

substrate: D-Thr


substrate_name: D-Ile

smiles: CCC(C)C(C(=O)O)N

substrate: D-Ile


substrate_name: D-Leu

substrate: D-Leu


substrate_name: D-Val

substrate: D-Val


substrate_name: D-Tyr

substrate: D-Tyr


enzyme_display_name: monoamine oxidase

organism: Huso huso

substrate_name: N-methylhistamine

enzyme: monoamine oxidase

substrate: N-methylhistamine


substrate_name: serotonin

substrate: serotonin


substrate_name: benzylamine

substrate: benzylamine


enzyme_display_name: pyridoxal 5'-phosphate synthase

organism: Oryctolagus cuniculus

substrate_name: pyridoxamine 5'-phosphate

proof_lines:
pH 8.0
enzyme activity in liver homogenate
pH 8.0, 37°C

enzyme: pyridoxal 5'-phosphate synthase

substrate: pyridoxamine 5'-phosphate


substrate_name: pyridoxine 5'-phosphate

proof_lines: pure enzyme in 200 mM potassium phosphate pH 7.0

substrate: pyridoxine 5'-phosphate


substrate_name: FMN

assay_conditions_summary: cofactor FMN

substrate: FMN


proof_lines: pure enzyme in 200 mM potassium phosphate pH 7.0 or enzyme activity in liver homogenate, substrate pyridoxamine 5' phosphate or pyridoxine 5'-phosphate


proof_lines:
cosubstrate pyridoxamine 5'-phosphate
cosubstrate pyridoxine 5'-phosphate


enzyme_display_name: D-amino acid dehydrogenase (quinone)

organism: Helicobacter pylori

substrate_name: oxidized coenzyme Q1

enzyme: D-amino acid dehydrogenase (quinone)

substrate: oxidized coenzyme Q1


substrate_name: D-proline

substrate: D-proline


enzyme_display_name: glutamate synthase (ferredoxin)

organism: Chlamydomonas reinhardtii

substrate_name: reduced ferredoxin

enzyme: glutamate synthase (ferredoxin)

substrate: reduced ferredoxin


substrate_name: L-glutamine

substrate: L-glutamine


substrate_name: methyl viologen

substrate: methyl viologen


enzyme_display_name: D-arginine dehydrogenase

organism: Pseudomonas aeruginosa

substrate_name: D-threonine

enzyme: D-arginine dehydrogenase

substrate: D-threonine


substrate_name: D-alanine

substrate: D-alanine


substrate_name: D-valine

substrate: D-valine


substrate_name: D-serine

substrate: D-serine


substrate_name: D-histidine

substrate: D-histidine


substrate_name: D-phenylalanine

substrate: D-phenylalanine


substrate_name: D-tyrosine

substrate: D-tyrosine


substrate_name: D-arginine

smiles: C(CC(C(=O)O)N)CN=C(N)N

substrate: D-arginine


substrate_name: D-leucine

proof_lines:
wild-type, pH 6.0, 25°C
wild-type, pH 10.0, 25°C

substrate: D-leucine


proof_lines: wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication

proof_lines: wild-type, pH-independent limiting value at high pH, pH not specified in the publication, 6°C


substrate_name: D-methionine

substrate: D-methionine


substrate_name: D-Lysine

substrate: D-Lysine


proof_lines: mutant E87L, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant H48F, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication


substrate_name: D-asparagine

substrate: D-asparagine


substrate_name: D-glutamine

substrate: D-glutamine


substrate_name: D-isoleucine

substrate: D-isoleucine


substrate_name: D-tryptophan

substrate: D-tryptophan


substrate_name: D-ornithine

smiles: C(CC(C(=O)O)N)CN

substrate: D-ornithine


proof_lines:
mutant S45A, pH 5.5, 25°C
mutant S45A, pH 10.0, 25°C


proof_lines:
mutant A46G, pH 6.5, 25°C
mutant A46G, pH 10.0, 25°C


organism: Mus musculus


substrate_name: NAD+

assay_conditions_summary: cofactor NAD+

substrate: NAD+


substrate_name: phosphate

substrate: phosphate


substrate_name: arsenate

substrate: arsenate


enzyme_display_name: pyrroline-5-carboxylate reductase

organism: Glycine max

substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

enzyme: pyrroline-5-carboxylate reductase

substrate: NADPH


substrate_name: NADH

substrate: NADH


proof_lines:
with NADPH
with NADH


organism: Medicago truncatula

assay_conditions_summary: pH 7.5; 308.15 K; cofactor NADPH


assay_conditions_summary: pH 7.5; 308.15 K; cofactor NADH


proof_lines:
cosubstrate NADPH, pH 7.5, 35°C
cosubstrate NADH, pH 7.5, 35°C


enzyme_display_name: dihydrofolate reductase

organism: Brugia malayi

proof_lines: above, pH 6.0, 25°C recombinant His-tagged enzyme

enzyme: dihydrofolate reductase


substrate_name: Unknown

proof_lines: pH 6.0, 25°C recombinant His-tagged enzyme

substrate: Unknown


organism: Gallus gallus

assay_conditions_summary: pH 7.4; cofactor NADPH

proof_lines:
pH 7.4
with NADPH


substrate_name: folate

substrate: folate


assay_conditions_summary: pH 5


organism: Geobacillus stearothermophilus

assay_conditions_summary: pH 7; 333.15 K; cofactor NADPH


organism: Heliothis virescens


organism: Lacticaseibacillus casei


assay_conditions_summary: pH 6.5; cofactor NADPH


organism: Wuchereria bancrofti


enzyme_display_name: flavin reductase (NADH)

organism: Acinetobacter baylyi

substrate_name: FMN

assay_conditions_summary: pH 7.5; 298.15 K; cofactor FMN

proof_lines: pH 7.5, 25°C, recombinant enzyme, with NADH

enzyme: flavin reductase (NADH)

substrate: FMN


substrate_name: riboflavin

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADH

substrate: riboflavin


substrate_name: FAD

assay_conditions_summary: pH 7.5; 298.15 K; cofactor FAD

substrate: FAD


proof_lines: pH 7.5, 25°C, recombinant enzyme, with FAD


enzyme_display_name: FMN reductase (NADH)

organism: Aminobacter aminovorans

assay_conditions_summary: pH 7.8; 298.15 K; cofactor FMN

enzyme: FMN reductase (NADH)


assay_conditions_summary: pH 7.8; 298.15 K; cofactor NADH


organism: Neomoorella thermoacetica


substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

substrate: NADP+


organism: Blautia producta

assay_conditions_summary: pH 5.5; 310.15 K; cofactor NADH


enzyme_display_name: saccharopine dehydrogenase (NADP(+), L-lysine-forming)

organism: Rattus norvegicus


enzyme: saccharopine dehydrogenase (NADP(+), L-lysine-forming)


substrate_name: L-lysine

substrate: L-lysine


enzyme_display_name: sarcosine dehydrogenase

substrate_name: sarcosine

smiles: CNCC(=O)O

enzyme: sarcosine dehydrogenase

substrate: sarcosine


enzyme_display_name: dimethylglycine dehydrogenase

substrate_name: N,N-dimethylglycine

smiles: CN(C)CC(=O)O

proof_lines: pH 7.0, 30°C, recombinant holoenzyme, 2 Km-values

enzyme: dimethylglycine dehydrogenase

substrate: N,N-dimethylglycine


organism: Methanothermobacter thermautotrophicus

substrate_name: methylenetetrahydromethanopterin

substrate: methylenetetrahydromethanopterin


substrate_name: oxidized coenzyme F420

substrate: oxidized coenzyme F420


substrate_name: coenzyme F420

substrate: coenzyme F420


substrate_name: reduced coenzyme F420

substrate: reduced coenzyme F420


enzyme_display_name: NADPH--hemoprotein reductase

organism: Helianthus tuberosus

substrate_name: ferricytochrome c

proof_lines: determined on the basis of a sequential mechanism

enzyme: NADPH--hemoprotein reductase

substrate: ferricytochrome c


organism: Phanerodontia chrysosporium

assay_conditions_summary: pH 7.8; 298.15 K; cofactor NADPH


mutation_signature: truncated enzyme

mutation_type: truncation


assay_conditions_summary: pH 7.5; 298.15 K; buffer 20 mM HEPES; cofactor NADPH

proof_lines: wild type enzyme, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C

proof_lines: wild type enzyme, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C

proof_lines: wild type enzyme, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C


assay_conditions_summary: pH 7.5; 298.15 K; buffer 20 mM HEPES

proof_lines: mutant enzyme D113A, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C

proof_lines: mutant enzyme D113A, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C

proof_lines: mutant enzyme D113A, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C


proof_lines: mutant enzyme E115A/E116A, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C

proof_lines: mutant enzyme E115A/E116A, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C

proof_lines: mutant enzyme E115A/E116A, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C


substrate_name: cytochrome c

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADPH

proof_lines:
aortic microsomal enzyme
purified enzyme
native enzyme, liver

substrate: cytochrome c


substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

proof_lines: native enzyme, liver, cosubstrate cytochrome c

substrate: NADPH


proof_lines: Y140F/178F double mutant, cosubstrate cytochrome c


proof_lines:
Y140F/178F double mutant
Y140F mutant


mutation_signature: deletion mutant T236/G237/E238/E239

mutation_type: deletion


mutation_signature: deletion mutant T236/G237


smiles: COC(=O)N


substrate_name: ethanol

smiles: CCO

substrate: ethanol


enzyme_display_name: NADPH dehydrogenase (quinone)

enzyme: NADPH dehydrogenase (quinone)


enzyme_display_name: NAD(P)H dehydrogenase (quinone)

organism: Beta vulgaris

assay_conditions_summary: pH 7.2; 298.15 K; buffer 10 mM bis-Tris-propane

proof_lines: in 10 mM bis-Tris-propane buffer (pH 7.2), at 25°C

enzyme: NAD(P)H dehydrogenase (quinone)


substrate_name: NADH

assay_conditions_summary: pH 7.2; 298.15 K; buffer 10 mM bis-Tris-propane; cofactor NADH

substrate: NADH


organism: Mus musculus

assay_conditions_summary: cofactor FAD

proof_lines:
hydrophobic isoform
hydrophilic isoform


substrate_name: menadione

proof_lines:
hydrophilic isoform
hydrophobic isoform

substrate: menadione


assay_conditions_summary: cofactor NADH


substrate_name: nitracrine

substrate: nitracrine


assay_conditions_summary: pH 7


assay_conditions_summary: pH 7; 298.15 K; cofactor NADH

proof_lines:
pH 7.0
pH 7.0, 25°C
competitive vs. NADH


proof_lines:
at pH 7.0 and 27°C
pH 7.0, 25°C
pH 7.0


substrate_name: mitomycin C

substrate: mitomycin C


substrate_name: vitamin K1

substrate: vitamin K1


substrate_name: chromate(VI)

substrate: chromate(VI)


enzyme_display_name: NADPH:quinone reductase

organism: Trypanosoma cruzi

substrate_name: nifurtimox

enzyme: NADPH:quinone reductase

substrate: nifurtimox


substrate_name: komaroviquinone

substrate: komaroviquinone


organism: Yarrowia lipolytica

proof_lines: pH and temperature not specified in the publication


enzyme_display_name: nitrate reductase (NADH)

organism: Solanum lycopersicum

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADH

proof_lines:
pH 7.5, 30°C, membrane-bound isoform
pH 7.5, 30°C, cytosolic isoform

enzyme: nitrate reductase (NADH)


substrate_name: nitrate

proof_lines:
pH 7.5, 30°C, cytosolic isoform
pH 7.5, 30°C, membrane-bound isoform

substrate: nitrate


enzyme_display_name: FMN-dependent NADH-azoreductase

organism: Halalkalibacter wakoensis

substrate_name: Orange I

enzyme: FMN-dependent NADH-azoreductase

substrate: Orange I


enzyme_display_name: azobenzene reductase

organism: Enterococcus faecalis

substrate_name: Methyl red

enzyme: azobenzene reductase

substrate: Methyl red


substrate_name: FMN

assay_conditions_summary: cofactor FMN

substrate: FMN


assay_conditions_summary: pH 7.1; 296.15 K; cofactor NADH


enzyme_display_name: GMP reductase

organism: Klebsiella aerogenes

substrate_name: GMP

enzyme: GMP reductase

substrate: GMP


enzyme_display_name: nitrite reductase (NO-forming)

organism: Hyphomicrobium denitrificans

substrate_name: nitrite

enzyme: nitrite reductase (NO-forming)

substrate: nitrite


enzyme_display_name: nitrite reductase (cytochrome; ammonia-forming)

organism: Trichlorobacter lovleyi

proof_lines: pH 7, temperature not specified in the publication


enzyme_display_name: trimethylamine-N-oxide reductase

organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: Trimethylamine N-oxide

enzyme: trimethylamine-N-oxide reductase

substrate: Trimethylamine N-oxide


substrate_name: Chlorate

substrate: Chlorate


enzyme_display_name: nitric-oxide reductase (cytochrome c)

organism: Paracoccus denitrificans

substrate_name: nitric oxide

enzyme: nitric-oxide reductase (cytochrome c)

substrate: nitric oxide


substrate_name: NO

smiles: [No]

substrate: NO


enzyme_display_name: adenylyl-sulfate reductase

organism: Desulfovibrio desulfuricans

substrate_name: AMP

enzyme: adenylyl-sulfate reductase

substrate: AMP


substrate_name: sulfite

substrate: sulfite


organism: Thiobacillus denitrificans


organism: Bos taurus

substrate_name: N-acetyl-S-(E,E)-farnesyl-L-cysteine

substrate: N-acetyl-S-(E,E)-farnesyl-L-cysteine


substrate_name: N-acetyl-S-geranylgeranyl-L-cysteine

substrate: N-acetyl-S-geranylgeranyl-L-cysteine


substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


organism: Lathyrus oleraceus

substrate_name: homocysteine

smiles: C(CS)C(C(=O)O)N

substrate: homocysteine


enzyme_display_name: 24-methylenesterol C-methyltransferase

organism: Glycine max

proof_lines:
wild-type enzyme
wild-type enzyme and mutant enzyme Y83F

enzyme: 24-methylenesterol C-methyltransferase


substrate_name: 24(28)-methylene lophenol

substrate: 24(28)-methylene lophenol


substrate_name: Cycloartenol

substrate: Cycloartenol


substrate_name: Unknown

proof_lines: substrate 24(28)-methylene lophenol, pH 7.5, 35°C

substrate: Unknown


organism: Saccharolobus solfataricus

substrate_name: SNAP-Vista Green

substrate: SNAP-Vista Green


enzyme_display_name: thiopurine S-methyltransferase

organism: Homo sapiens

enzyme: thiopurine S-methyltransferase


proof_lines: mutant L69V, cytosolic fraction of yeast cells is incubated with 500 microM of 6-TG and 0-400 microM of SAM

proof_lines: mutant L69V, cytosolic fraction of yeast cells is incubated with 0-350 microM of 6-TG and 1mM of SAM


proof_lines: mutant C212R, cytosolic fraction of yeast cells is incubated with 0-350 microM of 6-TG and 1mM of SAM


proof_lines: mutant Q179H, cytosolic fraction of yeast cells is incubated with 0-350 microM of 6-TG and 1mM of SAM


substrate_name: 6-thioinosine 5'-monophosphate

substrate: 6-thioinosine 5'-monophosphate


proof_lines: 1.2fold increase in KM-value compared to wild-type variant

proof_lines: wild-type enzyme, cytosolic fraction of yeast cells is incubated with 500 microM of 6-TG and 0-400 microM of SAM

proof_lines: 2.9fold increase in KM-value compared to wild-type variant


substrate_name: tellurite

substrate: tellurite


substrate_name: methanetellurol

smiles: C[Te]

substrate: methanetellurol


smiles: C(CS)O


organism: Methanopyrus kandleri

proof_lines:
pH 8.0, 65°C
recombinant enzyme


substrate_name: formylmethanofuran

proof_lines:
recombinant enzyme
pH 8.0, 65°C

substrate: formylmethanofuran


enzyme_display_name: [Wnt protein] O-palmitoleoyl transferase

assay_conditions_summary: pH 7.5; 310.15 K; cofactor CoA


enzyme_display_name: [ribosomal protein bS18]-alanine N-acetyltransferase


enzyme_display_name: fatty-acid synthase system

organism: Rattus norvegicus

substrate_name: NADH

proof_lines:
wild-type, enoylreductase activity
wild-type, beta-ketoreductase activity

enzyme: fatty-acid synthase system

substrate: NADH


substrate_name: NADPH

proof_lines: mutant G1886F, beta-ketoreductase activity

substrate: NADPH


proof_lines: mutant G1888A, beta-ketoreductase activity


substrate_name: malonyl-CoA

assay_conditions_summary: cofactor CoA

substrate: malonyl-CoA


substrate_name: acetyl-CoA

substrate: acetyl-CoA


organism: Aspergillus fumigatus

substrate_name: myristoleoyl-CoA

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: myristoleoyl-CoA


organism: Trypanosoma brucei

substrate_name: GCGGSKVK

substrate: GCGGSKVK


substrate_name: myristoyl-CoA

substrate: myristoyl-CoA


enzyme_display_name: hydroxymethylglutaryl-CoA synthase

organism: Diploptera punctata

enzyme: hydroxymethylglutaryl-CoA synthase


enzyme_display_name: 2-methylcitrate synthase

organism: Fusarium verticillioides

substrate_name: propionyl-CoA

enzyme: 2-methylcitrate synthase

substrate: propionyl-CoA


substrate_name: oxaloacetate

proof_lines: propionyl-CoA acetyl-CoA

substrate: oxaloacetate


assay_conditions_summary: pH 8; 303.15 K; cofactor CoA


substrate_name: propanoyl-CoA

substrate: propanoyl-CoA


proof_lines: using acetyl-CoA as cosubstrate, in 50 mM HEPES pH 8.0, at 30°C

proof_lines: using propanoyl-CoA as cosubstrate, in 50 mM HEPES pH 8.0, at 30°C


enzyme_display_name: ATP citrate synthase

substrate_name: CoA

assay_conditions_summary: cofactor ATP

proof_lines:
direct assay
coupled assay

enzyme: ATP citrate synthase

substrate: CoA


substrate_name: ATP

substrate: ATP


substrate_name: Unknown

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP

substrate: Unknown


assay_conditions_summary: pH 8; 295.15 K; cofactor ATP

proof_lines: wild type enzyme, average value, in 50 mM Tris, 10 mM MgCl2, and 2 mM dithiothreitol (pH 8.0), at 22°C


substrate_name: citrate

substrate: citrate


proof_lines: pH and temperature not specified in the publication


substrate_name: ADP

substrate: ADP


enzyme_display_name: malate synthase

organism: Corynebacterium glutamicum

enzyme: malate synthase


substrate_name: glyoxylate

substrate: glyoxylate


organism: Streptomyces clavuligerus


enzyme_display_name: inositol 3-alpha-galactosyltransferase

organism: Phaseolus vulgaris

substrate_name: UDP-galactose

enzyme: inositol 3-alpha-galactosyltransferase

substrate: UDP-galactose


substrate_name: myo-inositol

substrate: myo-inositol


enzyme_display_name: sterol 3beta-glucosyltransferase

organism: Kluyveromyces lactis

substrate_name: cholesterol

enzyme: sterol 3beta-glucosyltransferase

substrate: cholesterol


organism: Salmonella enterica subsp. enterica serovar Typhimurium


substrate_name: L-glutamine

smiles: C(CC(=O)N)C(C(=O)O)N

substrate: L-glutamine


organism: Oryctolagus cuniculus

smiles: C(CC(=O)O)CN


proof_lines:
competitive inhibition at 37°C
mixed inhibition at 37°C


substrate_name: beta-Alanine

smiles: C(CN)C(=O)O

substrate: beta-Alanine


smiles: CC(CN)C(=O)O


proof_lines:
37°C, pH 8.4
38°C, pH 8.5
synaptosomal enzyme, pH 8.0


proof_lines:
38°C, pH 8.5
mitochondrial enzyme
pH 8.0


organism: Streptomyces griseus


enzyme_display_name: D-amino-acid transaminase

organism: Bacillus sp. (in: firmicutes)

proof_lines:
pH 8.0, 37°C, wild-type enzyme
pH 8.5, 50°C, wild-type enzyme

enzyme: D-amino-acid transaminase


substrate_name: D-alanine

smiles: CC(C(=O)O)N

proof_lines: pH 8.5, 50°C, wild-type and E32Q mutant enzyme

substrate: D-alanine


smiles: CC(C)C(=O)C(=O)O


enzyme_display_name: hexokinase

organism: Leishmania mexicana

substrate_name: D-glucose

enzyme: hexokinase

substrate: D-glucose


substrate_name: D-fructose

substrate: D-fructose


proof_lines:
inhibition with respect to ATP
inhibition with respect to glucose


organism: Bacillus methanolicus

assay_conditions_summary: pH 9; 303.15 K; cofactor ATP


substrate_name: D-fructose 6-phosphate

substrate: D-fructose 6-phosphate


organism: Geobacillus stearothermophilus

substrate_name: fructose 6-phosphate

smiles: C(C(C(C(C(=O)CO)O)O)O)OP(=O)(O)O

proof_lines:
forward reaction, recombinant enzyme
pH 8.2, 30°C, native PFK

substrate: fructose 6-phosphate


assay_conditions_summary: pH 8.2; 303.15 K; cofactor ATP

proof_lines:
pH 8.2, 30°C
pH 8.2, 30°C, native PFK


proof_lines: pH 8.2, 30°C, at fructose 6-phosphate concentrations that are less than approx. one-third the fixed MgATP concentration


substrate_name: fructose 1,6-bisphosphate

substrate: fructose 1,6-bisphosphate


substrate_name: GTP

substrate: GTP


substrate_name: CTP

substrate: CTP


substrate_name: UTP

substrate: UTP


assay_conditions_summary: pH 8; 298.15 K; cofactor ATP


substrate_name: ATP

substrate: ATP


enzyme_display_name: glucokinase

organism: Streptococcus mutans

assay_conditions_summary: pH 7.5; 295.15 K; cofactor ATP

enzyme: glucokinase


enzyme_display_name: ketohexokinase

organism: Homo sapiens

substrate_name: D-ribose

proof_lines:
25°C, pH 7, ketohexokinase-A
25°C, pH 7, ketohexokinase-C

enzyme: ketohexokinase

substrate: D-ribose


proof_lines:
25°C, pH 7, C-A43T
25°C, pH 7, A-A43T
25°C, pH 7, ketohexokinase-C A43T


substrate_name: D-ribulose

smiles: C(C(C(C(=O)CO)O)O)O

proof_lines:
25°C, pH 7, ketohexokinase-C
25°C, pH 7, ketohexokinase-A
37°C, pH 7.5

substrate: D-ribulose


substrate_name: L-sorbose

substrate: L-sorbose


assay_conditions_summary: pH 7; 298.15 K; cofactor ATP

proof_lines:
25°C, pH 7, ketohexokinase-C wild type
25°C, pH 7, ketohexokinase-A wild type


proof_lines:
25°C, pH 7, ketohexokinase-C A43T
25°C, pH 7, ketohexokinase-A A43T


substrate_name: D-xylose

substrate: D-xylose


organism: Rattus norvegicus

assay_conditions_summary: pH 7.4; cofactor ATP

proof_lines:
pH 7.4, cosubstrate GTP
pH 7.4, + ATP


enzyme_display_name: fructokinase

organism: Leuconostoc mesenteroides

enzyme: fructokinase


substrate_name: D-mannose

substrate: D-mannose


enzyme_display_name: galactokinase

organism: Trypanosoma cruzi

substrate_name: D-galactose

assay_conditions_summary: pH 8.6; cofactor ATP

proof_lines: pH 8.6, temperature not specified in the publication

enzyme: galactokinase

substrate: D-galactose


proof_lines: isoform GalK2, pH 8.6, temperature not specified in the publication


enzyme_display_name: pyruvate, phosphate dikinase

organism: Acetobacter aceti

substrate_name: AMP

enzyme: pyruvate, phosphate dikinase

substrate: AMP


substrate_name: pyruvate

proof_lines: pH 8, 30°C, phosphoenolpyruvate formation

substrate: pyruvate


substrate_name: diphosphate

substrate: diphosphate


substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


assay_conditions_summary: pH 8; 303.15 K; cofactor ATP


substrate_name: phosphate

substrate: phosphate


substrate_name: Unknown

substrate: Unknown


organism: Komagataeibacter xylinus


proof_lines: pH 8.2, 30°C, phosphoenolpyruvate formation


organism: Clostridium sporogenes

substrate_name: cinnamoyl-CoA

substrate: cinnamoyl-CoA


substrate_name: phenylpropionate

substrate: phenylpropionate


enzyme_display_name: tRNA 2-selenouridine synthase

substrate_name: Selenophosphate

enzyme: tRNA 2-selenouridine synthase

substrate: Selenophosphate


organism: Aspergillus fumigatus

substrate_name: benzyl D-glucuronate

substrate: benzyl D-glucuronate


substrate_name: 3-(4-methoxyphenyl) propyl methyl 4-O-methyl-alpha-D-glucopyranosiduronate


organism: Gelatoporia subvermispora

substrate_name: benzyl glucuronic acid

substrate: benzyl glucuronic acid


enzyme_display_name: acetylxylan esterase

organism: Aspergillus awamori

substrate_name: 1-naphthyl acetate

proof_lines: substrate: alpha-naphthylacetate, wild-type enzyme

enzyme: acetylxylan esterase

substrate: 1-naphthyl acetate


enzyme_display_name: feruloyl esterase

organism: Butyrivibrio proteoclasticus

substrate_name: p-nitrophenyl acetate

assay_conditions_summary: pH 7.2; 310.15 K; buffer 100 mM phosphate

proof_lines: in 100 mM phosphate buffer, pH 7.2, at 37°C

proof_lines: pH and temperature not specified in the publication

enzyme: feruloyl esterase

substrate: p-nitrophenyl acetate


substrate_name: p-nitrophenyl butyrate

substrate: p-nitrophenyl butyrate


organism: Neurospora crassa

substrate_name: methyl p-coumarate

substrate: methyl p-coumarate


substrate_name: methyl caffeate

substrate: methyl caffeate


substrate_name: methyl ferulate

substrate: methyl ferulate


organism: Penicillium expansum


substrate_name: methyl coumarate

proof_lines:
p-coumaroyl esterase
37°C, pH 6.0

substrate: methyl coumarate


organism: Talaromyces pinophilus

substrate_name: 4-nitrophenyl acetate

substrate: 4-nitrophenyl acetate


substrate_name: 4-nitrophenyl ferulate

substrate: 4-nitrophenyl ferulate


organism: uncultured bacterium

substrate_name: methyl 4-coumarate

substrate: methyl 4-coumarate


substrate_name: methyl sinapate

substrate: methyl sinapate


substrate_name: 4-nitrophenyl butyrate

substrate: 4-nitrophenyl butyrate


enzyme_display_name: cutinase

organism: Thermothielavioides terrestris

substrate_name: 4-nitrophenyl caproate

enzyme: cutinase

substrate: 4-nitrophenyl caproate


substrate_name: 4-nitrophenyl butanoate

substrate: 4-nitrophenyl butanoate


substrate_name: 4-nitrophenyl dodecanoate

substrate: 4-nitrophenyl dodecanoate


substrate_name: 4-nitrophenyl hexadecanoate

substrate: 4-nitrophenyl hexadecanoate


substrate_name: 4-nitrophenyl octanoate

substrate: 4-nitrophenyl octanoate


enzyme_display_name: poly(3-hydroxybutyrate) depolymerase

organism: Acidovorax sp.

substrate_name: cyclic [(R)-3-hydroxybutanoate]5

proof_lines:
cyclic 3-hydroxybutyrate oligomer
linear 3-hydroxybutyrateoligomer
linear 3-hydroxybutyrate oligomer

enzyme: poly(3-hydroxybutyrate) depolymerase

substrate: cyclic [(R)-3-hydroxybutanoate]5


enzyme_display_name: acyl-CoA hydrolase

organism: Cereibacter sphaeroides

substrate_name: hexadecanoyl-CoA

assay_conditions_summary: pH 7.8; 295.15 K; cofactor CoA

enzyme: acyl-CoA hydrolase

substrate: hexadecanoyl-CoA


substrate_name: octadecanoyl-CoA

substrate: octadecanoyl-CoA


substrate_name: tetradecanoyl-CoA

substrate: tetradecanoyl-CoA


substrate_name: dodecanoyl-CoA

substrate: dodecanoyl-CoA


substrate_name: decanoyl-CoA

substrate: decanoyl-CoA


substrate_name: hexanoyl-CoA

substrate: hexanoyl-CoA


substrate_name: octanoyl-CoA

substrate: octanoyl-CoA


substrate_name: butyryl-CoA

substrate: butyryl-CoA


enzyme_display_name: type II site-specific deoxyribonuclease

organism: Haemophilus parainfluenzae

enzyme: type II site-specific deoxyribonuclease


enzyme_display_name: nocturnin

organism: Mus musculus

substrate_name: NADPH

assay_conditions_summary: 295.15 K; cofactor NADPH

proof_lines: 22°C, pH not specified in the publication

enzyme: nocturnin

substrate: NADPH


organism: Bacteroides thetaiotaomicron

substrate_name: KDN 9-phosphate

substrate: KDN 9-phosphate


enzyme_display_name: protein-tyrosine-phosphatase

organism: Bdellovibrio bacteriovorus

substrate_name: Inositol hexakisphosphate

proof_lines: in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C

enzyme: protein-tyrosine-phosphatase

substrate: Inositol hexakisphosphate


organism: Gallus gallus

substrate_name: myo-inositol hexakisphosphate

substrate: myo-inositol hexakisphosphate


substrate_name: scyllo-inositol hexakisphosphate

substrate: scyllo-inositol hexakisphosphate


organism: Homo sapiens


enzyme_display_name: aryldialkylphosphatase

organism: Saccharolobus solfataricus

substrate_name: paraoxon

assay_conditions_summary: pH 8; 343.15 K; buffer 100 mM sodium phosphate

proof_lines: recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C

enzyme: aryldialkylphosphatase

substrate: paraoxon


proof_lines: pH 8.0, 70°C, mutant enzyme Y97W/I98F/I261F


proof_lines:
pH 8.0, 70°C, recombinant enzyme
pH 8.0, 70°C
70°C, pH 9.0


substrate_name: methyl paraoxon

proof_lines:
pH 8.0, 70°C, recombinant enzyme
25°C, pH 9.0

substrate: methyl paraoxon


substrate_name: malathion

smiles: CCOC(=O)CC(C(=O)OCC)SP(=S)(OC)OC

substrate: malathion


substrate_name: methyl parathion

substrate: methyl parathion


substrate_name: fensulfothion

substrate: fensulfothion


substrate_name: diethyl-paraoxon

proof_lines: recombinant enzyme mutant C258L/I261F/W263A, pH 8.5, 25°C, with 0.025% SDS

proof_lines: recombinant enzyme mutant C258L/I261F/W263A, pH 8.5, 65°C, with 0.025% SDS

substrate: diethyl-paraoxon


organism: Sphingobium sp. TCM1

substrate_name: dibutyl 3,3-difluorobutyl phosphate


substrate_name: 4-carbamoylphenyl diethyl phosphate

substrate: 4-carbamoylphenyl diethyl phosphate


substrate_name: diethyl 4-methoxyphenyl phosphate

substrate: diethyl 4-methoxyphenyl phosphate


substrate_name: dibutyl phenyl phosphate

substrate: dibutyl phenyl phosphate


substrate_name: dibutyl 4-nitrophenyl phosphate

substrate: dibutyl 4-nitrophenyl phosphate


substrate_name: diethyl 4-formylphenyl phosphate

substrate: diethyl 4-formylphenyl phosphate


substrate_name: diethyl 4-chlorophenyl phosphate

substrate: diethyl 4-chlorophenyl phosphate


substrate_name: diethyl phenyl phosphate

substrate: diethyl phenyl phosphate


substrate_name: 4-acetylphenyl cyclohexyl methyl (R)-phosphate


substrate_name: cyclohexyl methyl phenyl (R)-phosphate

proof_lines:
pH 8.5, 30°C
at pH 9.0 and 30°C

substrate: cyclohexyl methyl phenyl (R)-phosphate


substrate_name: diethyl 4-nitrophenyl phosphate

substrate: diethyl 4-nitrophenyl phosphate


substrate_name: methyl bis(4-nitrophenyl) phosphate

substrate: methyl bis(4-nitrophenyl) phosphate


substrate_name: dimethyl 4-nitrophenyl phosphate

substrate: dimethyl 4-nitrophenyl phosphate


substrate_name: (R)-dicyclohexyl phenyl phosphate

substrate: (R)-dicyclohexyl phenyl phosphate


substrate_name: (S)-dimethyl phenyl phosphate

substrate: (S)-dimethyl phenyl phosphate


substrate_name: methyl diphenyl phosphate

substrate: methyl diphenyl phosphate


substrate_name: cyclohexyl diphenyl phosphate

substrate: cyclohexyl diphenyl phosphate


substrate_name: 4-nitrophenyl diphenyl phosphate

substrate: 4-nitrophenyl diphenyl phosphate


substrate_name: (R)-bis(4-nitrophenyl) phenyl phosphate

substrate: (R)-bis(4-nitrophenyl) phenyl phosphate


substrate_name: tris(4-nitrophenyl) phosphate

substrate: tris(4-nitrophenyl) phosphate


substrate_name: triphenyl phosphate

substrate: triphenyl phosphate


organism: Rattus norvegicus

substrate_name: 4-nitrophenyl alpha-D-mannoside

proof_lines: intact 124 kDa Man II and chymotrypsin-cleaved 110 kDa catalytic subunit

substrate: 4-nitrophenyl alpha-D-mannoside


enzyme_display_name: cellulose 1,4-beta-cellobiosidase (non-reducing end)

organism: Trichoderma virens

substrate_name: Unknown

substrate: Unknown


substrate_name: 4-nitrophenyl beta-D-cellobioside

enzyme: cellulose 1,4-beta-cellobiosidase (non-reducing end)

substrate: 4-nitrophenyl beta-D-cellobioside


organism: Clostridium perfringens

proof_lines:
endo-beta-N-acetylglucosaminidase CI
endo-beta-N-acetylglucosaminidase CII


enzyme_display_name: leukotriene-A4 hydrolase

substrate_name: leukotriene A4

enzyme: leukotriene-A4 hydrolase

substrate: leukotriene A4


assay_conditions_summary: pH 7.6; 295.15 K; buffer 250 mM Tris


proof_lines:
value depending on NaSCN concentration
pH 8.0, 22°C
pH 7.7-8.0, 37°C


substrate_name: L-Ala-p-nitroanilide

assay_conditions_summary: pH 7.5; buffer 250 mM Tris

proof_lines: mutant enzyme E296Q, 500 mM KCl in 250 mM Tris buffer (pH 7.5)

substrate: L-Ala-p-nitroanilide


proof_lines: mutant enzyme D375A, 500 mM KCl in 250 mM Tris buffer (pH 7.5)


substrate_name: Arg-Ser-Arg

proof_lines: wild type enzyme, 500 mM KCl in 250 mM Tris buffer (pH 7.5)

substrate: Arg-Ser-Arg


proof_lines: pH 8.0, wild-type enzyme in comparison to mutant enzymes


enzyme_display_name: microsomal epoxide hydrolase

substrate_name: hepoxilin A3

enzyme: microsomal epoxide hydrolase

substrate: hepoxilin A3


substrate_name: 8,9-epoxyeicosatrienoic acid

substrate: 8,9-epoxyeicosatrienoic acid


substrate_name: 14,15-epoxyeicosatrienoic acid

substrate: 14,15-epoxyeicosatrienoic acid


substrate_name: 13,14-epoxy-docosapentaenoic acid

proof_lines:
pH 7.4, 37°C
pH 9.0, 37°C
pH 7.4, 37°C, liver microsomes

substrate: 13,14-epoxy-docosapentaenoic acid


substrate_name: cis-stilbene oxide

substrate: cis-stilbene oxide


substrate_name: styrene 7,8-oxide

substrate: styrene 7,8-oxide


smiles: CCCCCC=CCC=CCC=CCC=CCCCC(=O)OC(CO)CO


enzyme_display_name: cytosol alanyl aminopeptidase

substrate_name: Met-enkephalin

enzyme: cytosol alanyl aminopeptidase

substrate: Met-enkephalin


enzyme_display_name: methionyl aminopeptidase

organism: Vibrio parahaemolyticus

proof_lines: pH and temperature not specified in the publication

enzyme: methionyl aminopeptidase


enzyme_display_name: PepB aminopeptidase

organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: Glu-Leu

smiles: CC(C)CC(C(=O)O)NC(=O)C(CCC(=O)O)N

enzyme: PepB aminopeptidase

substrate: Glu-Leu


substrate_name: Leu-Leu

smiles: CC(C)CC(C(=O)NC(CC(C)C)C(=O)O)N

substrate: Leu-Leu


substrate_name: Asp-Leu-Gly

smiles: CC(C)CC(C(=O)NCC(=O)O)NC(=O)C(CC(=O)O)N

substrate: Asp-Leu-Gly


enzyme_display_name: tripeptidyl-peptidase II

organism: Drosophila melanogaster

enzyme: tripeptidyl-peptidase II


substrate_name: L-Ala-L-Ala-L-Ala 4-nitroanilide

proof_lines:
wild-type, pH 6.4, 37°C
wild-type, pH 7.6, 37°C
wild-type, pH 8.3, 37°C

substrate: L-Ala-L-Ala-L-Ala 4-nitroanilide


enzyme_display_name: Xaa-Pro dipeptidyl-peptidase

organism: Lacticaseibacillus casei

enzyme: Xaa-Pro dipeptidyl-peptidase


organism: Streptococcus mutans


enzyme_display_name: lysosomal Pro-Xaa carboxypeptidase

organism: Tenebrio molitor

enzyme: lysosomal Pro-Xaa carboxypeptidase


enzyme_display_name: carboxypeptidase A

substrate_name: Benzyloxycarbonyl-Gly-Phe

proof_lines:
carboxypeptidase 2
carboxypeptidase 1

enzyme: carboxypeptidase A

substrate: Benzyloxycarbonyl-Gly-Phe


enzyme_display_name: carboxypeptidase B

organism: Aedes aegypti

substrate_name: Unknown

enzyme: carboxypeptidase B

substrate: Unknown


proof_lines: mutant enzyme V242I/L243G, at pH 7.7 and 25°C


organism: Sus scrofa

substrate_name: hippuryl-Lys

substrate: hippuryl-Lys


substrate_name: furylacryloyl-L-alanyl-L-lysine

substrate: furylacryloyl-L-alanyl-L-lysine


substrate_name: hippuryl-L-Arg

proof_lines:
soluble enzyme
immobilized enzyme

substrate: hippuryl-L-Arg


substrate_name: furylacryloyl-L-alanyl-L-arginine

substrate: furylacryloyl-L-alanyl-L-arginine


substrate_name: hippuryl-Arg

substrate: hippuryl-Arg


enzyme_display_name: pancreatic elastase II

enzyme: pancreatic elastase II


enzyme_display_name: peptidase 1 (mite)

organism: Dermatophagoides farinae

substrate_name: casein

enzyme: peptidase 1 (mite)

substrate: casein


enzyme_display_name: renin

substrate_name: recombinant ovine angiotensinogen from CHO cells

proof_lines:
at pH 7.2 and 37°C
pH 8.0, 37°C
at pH 8.0 and 37°C

enzyme: renin


substrate_name: angiotensinogen

assay_conditions_summary: pH 7.4; 310.15 K; buffer porcine substrate

proof_lines:
at pH 7.4 and 37°C
pH 6.0, 37°C, maleate buffer
porcine substrate, activated enzyme

substrate: angiotensinogen


organism: Mus musculus


enzyme_display_name: penicillopepsin

organism: Penicillium janthinellum

substrate_name: Ac-Lys 4-nitrophenyl amide

proof_lines:
pH 5.5
pH 4.3 (pH-optimum for kcat)
pH 3.8-4.2 (pH-optimum for kcat)

enzyme: penicillopepsin

substrate: Ac-Lys 4-nitrophenyl amide


substrate_name: Trypsinogen

substrate: Trypsinogen


enzyme_display_name: ADAMTS-4 endopeptidase

organism: Bos taurus

proof_lines: biotinylated peptide containing aggrecanase cleavage site

enzyme: ADAMTS-4 endopeptidase


organism: Homo sapiens

proof_lines:
ADAMTS-4-2, at 25°C
in 100 mM NaCl
at pH 7.5 and 37°C


enzyme_display_name: GTP cyclohydrolase II

organism: Streptomyces coelicolor

substrate_name: GTP

assay_conditions_summary: pH 8

proof_lines: SCO 2687, wild type, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol

enzyme: GTP cyclohydrolase II

substrate: GTP


proof_lines: SCO 2687, variant G209D, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol


proof_lines: SCO 2687, variant R123M, 1 mM substrate, 0.1 M Tris-HCl, pH 8, 5 mM MgCl2, 0.5 mM dithiothreitol


enzyme_display_name: S-methyl-5'-thioadenosine deaminase

organism: Pseudomonas aeruginosa

substrate_name: adenosine

enzyme: S-methyl-5'-thioadenosine deaminase

substrate: adenosine


enzyme_display_name: mRNA(cytosine(6666)) deaminase

substrate_name: cytosine6666 in apolipoprotein B mRNA

proof_lines: 30°C, pH 7.0, APOBEC1 in presence of the auxiliary factor ACF

proof_lines: 41°C, pH 7.0, APOBEC1 in presence of the auxiliary factor ACF

proof_lines: 30°C, pH 7.5, APOBEC1 without auxiliary factor

enzyme: mRNA(cytosine(6666)) deaminase

substrate: cytosine6666 in apolipoprotein B mRNA


enzyme_display_name: glucosamine-6-phosphate deaminase

organism: Musca domestica

proof_lines: pH 8.5, 37°C, in presence of glucose 6-phosphate

enzyme: glucosamine-6-phosphate deaminase


substrate_name: D-fructose 6-phosphate

proof_lines: pH 8.5, 37°C, in presence of 10 mM glucose 6-phosphate

substrate: D-fructose 6-phosphate


substrate_name: D-glucosamine 6-phosphate

proof_lines: pH 8.5, 37°C, in absence of activators, or in presence of glucose 6-phosphate

substrate: D-glucosamine 6-phosphate


organism: Oryctolagus cuniculus

proof_lines: pH 8.2, 37°C, in presence of N-acetylglucosamine 6-phosphate and Mn2+


proof_lines: pH 8.2, 37°C, in presence of N-acetylglucosamine 6-phosphate


organism: Pasteurella multocida

substrate_name: alpha-D-glucosamine 6-phosphate

proof_lines: pH and temperature not specified in the publication

substrate: alpha-D-glucosamine 6-phosphate


enzyme_display_name: nucleoside-triphosphate phosphatase

assay_conditions_summary: cofactor ATP

proof_lines: concentration of MgATP2- higher than 0.006 mM

enzyme: nucleoside-triphosphate phosphatase


enzyme_display_name: nucleotide diphosphatase

organism: Saccharomyces sp.

substrate_name: NAD+

assay_conditions_summary: cofactor NAD+

enzyme: nucleotide diphosphatase

substrate: NAD+


substrate_name: GDP-mannose

substrate: GDP-mannose


substrate_name: UDP-glucose

substrate: UDP-glucose


substrate_name: thymidine 5'-monophosphate p-nitrophenyl ester


organism: Pseudomonas sp.


organism: Pseudomonas putida

substrate_name: meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-propylcatechol


substrate_name: 3-methylcatechol ring-fission product

substrate: 3-methylcatechol ring-fission product


smiles: CC(C)C(=O)C=CC=C(C(=O)O)O


enzyme_display_name: haloalkane dehalogenase

organism: Corynebacterium sp.

smiles: CCCCCl

enzyme: haloalkane dehalogenase


organism: Rhodococcus erythropolis

proof_lines:
lyophilized cells in soli-gas biofilter
30°C, whole cells


enzyme_display_name: pyruvate decarboxylase

organism: Gluconacetobacter diazotrophicus

substrate_name: pyruvate

proof_lines:
pH 5.0, 25°C
at pH 5.0 and 25°C
at pH 6.0 and 25°C

enzyme: pyruvate decarboxylase

substrate: pyruvate


organism: Neurospora crassa

proof_lines: kcat per monomer, pH 6.0, MES-NaOH buffer, 25°C


enzyme_display_name: 11beta-hydroxysteroid dehydrogenase

substrate_name: Cortisol; NAD+

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

assay_conditions_summary: pH 7.4; 301.15 K; cofactor NAD+

proof_lines: isoform 11beta-HSD1, at pH 7.4, temperature not specified in the publication

enzyme: 11beta-hydroxysteroid dehydrogenase

substrate: Cortisol; NAD+


enzyme_display_name: malate dehydrogenase (decarboxylating)

organism: Pisum sativum

substrate_name: NAD+; L-Malate

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

enzyme: malate dehydrogenase (decarboxylating)

substrate: NAD+; L-Malate


enzyme_display_name: glucose 1-dehydrogenase [NAD(P)(+)]

organism: Rattus norvegicus

substrate_name: beta-D-Glucose; NAD+

enzyme: glucose 1-dehydrogenase [NAD(P)(+)]

substrate: beta-D-Glucose; NAD+


organism: Lactococcus lactis subsp. lactis

substrate_name: Pyruvate; NAD+; Coenzyme A

substrate: Pyruvate; NAD+; Coenzyme A


organism: Scheffersomyces stipitis

substrate_name: Coenzyme A; Pyruvate; NAD+

substrate: Coenzyme A; Pyruvate; NAD+


substrate_name: NAD+; 3-Methyl-2-oxopentanoate; Coenzyme A


organism: Macaca fascicularis


enzyme_display_name: glutamate dehydrogenase [NAD(P)(+)]

substrate_name: L-Glutamate; H2O; NAD+

assay_conditions_summary: cofactor NADP+

proof_lines: molar ratio pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm)/GDH1: 1

proof_lines: molar ratio pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm)/GDH1: 2

enzyme: glutamate dehydrogenase [NAD(P)(+)]

substrate: L-Glutamate; H2O; NAD+


enzyme_display_name: dihydrofolate reductase

organism: Geobacillus stearothermophilus

substrate_name: Dihydrofolate; H+; NADPH

assay_conditions_summary: cofactor NADPH

enzyme: dihydrofolate reductase

substrate: Dihydrofolate; H+; NADPH


enzyme_display_name: 6,7-dihydropteridine reductase

organism: Leishmania major

substrate_name: NADH; Quinonoid 6,7-dimethyl dihydropterin; H+

enzyme: 6,7-dihydropteridine reductase


organism: Bos taurus

proof_lines: inhibition of N-acetyl-S-(E,E)-farnesyl-L-cysteine methylation


organism: Chlorocebus sp.

substrate_name: D-Fructose 6-phosphate; ATP

substrate: D-Fructose 6-phosphate; ATP


organism: Lactobacillus casei


enzyme_display_name: glucokinase

organism: Streptococcus mutans

substrate_name: Glucose; ATP

enzyme: glucokinase

substrate: Glucose; ATP


enzyme_display_name: galactokinase

substrate_name: ATP; D-Galactose

enzyme: galactokinase

substrate: ATP; D-Galactose


enzyme_display_name: poly(3-hydroxybutyrate) depolymerase

organism: Acidovorax sp. sa1

enzyme: poly(3-hydroxybutyrate) depolymerase


enzyme_display_name: carnitine 3-dehydrogenase

organism: Rhizobium sp. YS-240

substrate_name: NADH

assay_conditions_summary: cofactor NADH

enzyme: carnitine 3-dehydrogenase

substrate: NADH


substrate_name: NAD+


substrate_name: L-carnitine

substrate: L-carnitine


enzyme_display_name: gluconate 2-dehydrogenase

organism: Acetobacter pasteurianus

substrate_name: Hydroxypyruvate

enzyme: gluconate 2-dehydrogenase

substrate: Hydroxypyruvate


substrate_name: glyoxylate

substrate: glyoxylate


substrate_name: D-gluconate

substrate: D-gluconate


enzyme_display_name: malate dehydrogenase (NADP(+))

organism: Spinacia oleracea

substrate_name: oxaloacetate

enzyme: malate dehydrogenase (NADP(+))

substrate: oxaloacetate


substrate_name: NADPH

substrate: NADPH


enzyme_display_name: trans-cinnamate 4-monooxygenase

organism: Cucumis sativus

substrate_name: trans-cinnamate

enzyme: trans-cinnamate 4-monooxygenase

substrate: trans-cinnamate


enzyme_display_name: xanthine oxidase

organism: Mus musculus

substrate_name: xanthine

enzyme: xanthine oxidase

substrate: xanthine


enzyme_display_name: fumarate reductase (NADH)

organism: Fibrobacter succinogenes

substrate_name: Unknown

enzyme: fumarate reductase (NADH)

substrate: Unknown


enzyme_display_name: Delta(24)-sterol reductase

organism: Manduca sexta

substrate_name: Cycloartenol

enzyme: Delta(24)-sterol reductase

substrate: Cycloartenol


organism: Gallus gallus


enzyme_display_name: NADPH--hemoprotein reductase

organism: Rattus norvegicus

substrate_name: hexadecanal

smiles: CCCCCCCCCCCCCCCC=O

enzyme: NADPH--hemoprotein reductase

substrate: hexadecanal


substrate_name: octanal

smiles: CCCCCCCC=O

substrate: octanal


substrate_name: Benzalacetone

substrate: Benzalacetone


organism: Lathyrus oleraceus


substrate_name: S-methylmethionine

substrate: S-methylmethionine


substrate_name: S-adenosylmethionine

substrate: S-adenosylmethionine


enzyme_display_name: malate synthase

organism: Gossypium hirsutum

substrate_name: acetyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: malate synthase

substrate: acetyl-CoA


enzyme_display_name: sinapate 1-glucosyltransferase

organism: Raphanus sativus

substrate_name: Sinapic acid

enzyme: sinapate 1-glucosyltransferase

substrate: Sinapic acid


substrate_name: UDPglucose

substrate: UDPglucose


enzyme_display_name: monoglucosyldiacylglycerol synthase

organism: Trichormus variabilis

enzyme: monoglucosyldiacylglycerol synthase


enzyme_display_name: feruloyl esterase

organism: Talaromyces pinophilus

substrate_name: p-coumaric acid methyl ester

enzyme: feruloyl esterase

substrate: p-coumaric acid methyl ester


enzyme_display_name: acyl-CoA hydrolase

organism: Cereibacter sphaeroides

substrate_name: vaccenoyl-CoA

enzyme: acyl-CoA hydrolase

substrate: vaccenoyl-CoA


substrate_name: linoleoyl-CoA

substrate: linoleoyl-CoA


substrate_name: oleoyl-CoA

substrate: oleoyl-CoA


substrate_name: myristoleoyl-CoA

substrate: myristoleoyl-CoA


enzyme_display_name: haloacetate dehalogenase

organism: Agrobacterium sp.

substrate_name: Monochloroacetate

enzyme: haloacetate dehalogenase

substrate: Monochloroacetate


enzyme_display_name: phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating)

organism: Geobacillus stearothermophilus

substrate_name: NADP+; 6-Phospho-D-gluconate

substrate: NADP+; 6-Phospho-D-gluconate


enzyme_display_name: 3-dehydrosphinganine reductase

organism: Candida albicans

smiles: CCCCCCCCCCCCCCCC(=O)C(CO)N

enzyme: 3-dehydrosphinganine reductase


substrate_name: NAD+

proof_lines: L-carnitine dehydrogenase, two different forms of the enzyme or two binding sites

substrate: NAD+


substrate_name: NADH

substrate: NADH


substrate_name: L-carnitine

substrate: L-carnitine


organism: Oryctolagus cuniculus

substrate_name: prostaglandin E1

substrate: prostaglandin E1


substrate_name: prostaglandin A1

substrate: prostaglandin A1


substrate_name: prostaglandin E2

substrate: prostaglandin E2


substrate_name: prostaglandin F2alpha

substrate: prostaglandin F2alpha


substrate_name: Prostaglandin D2

substrate: Prostaglandin D2


smiles: CCCCCC(C=CC=CCC=CCCCC(=O)O)O


substrate_name: 15-hydroxyeicosatetraenoic acid

smiles: CCCCCC(CCCCCC=CC=CC=CC=CC(=O)O)O

substrate: 15-hydroxyeicosatetraenoic acid


substrate_name: 19-hydroxyprostaglandin E1

substrate: 19-hydroxyprostaglandin E1


substrate_name: prostaglandin E3

substrate: prostaglandin E3


substrate_name: 20-hydroxyprostaglandin E1

substrate: 20-hydroxyprostaglandin E1


substrate_name: 6-ketoprostaglandin E1

substrate: 6-ketoprostaglandin E1


substrate_name: 6-ketoprostaglandin F1alpha

substrate: 6-ketoprostaglandin F1alpha


substrate_name: androstanediol

proof_lines:
3betaHSD I
3betaHSD III

substrate: androstanediol


substrate_name: stanolone

substrate: stanolone


substrate_name: pregnenolone

proof_lines:
3betaHSD VI
3betaHSD I
3betaHSD III

substrate: pregnenolone


substrate_name: dehydroepiandrosterone

substrate: dehydroepiandrosterone


enzyme_display_name: 11beta-hydroxysteroid dehydrogenase

proof_lines:
recombinant enzyme, at pH 7.4 and 37°C
pH 7.4, 37°C
native enzyme, at pH 7.4 and 37°C

enzyme: 11beta-hydroxysteroid dehydrogenase


substrate_name: corticosterone

proof_lines:
recombinant enzyme, at pH 7.4 and 37°C
native enzyme, at pH 7.4 and 37°C

substrate: corticosterone


substrate_name: Unknown

proof_lines: 82% inhibition at 0.01 mM, cell-based assay, pH not specified in the publication, temperature not specified in the publication

proof_lines: 75% inhibition at 0.01 mM, cell-based assay, pH not specified in the publication, temperature not specified in the publication

proof_lines: 85% inhibition at 0.01 mM, cell-based assay, pH not specified in the publication, temperature not specified in the publication

substrate: Unknown


enzyme_display_name: sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming)

substrate_name: 9,10-phenanthrene quinone

proof_lines:
pH 7.4, 37°C
pH 6.4, 25°C
pH 6.4, 25°C, erythrocyte enzyme

substrate: 9,10-phenanthrene quinone


substrate_name: benzil

enzyme: sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming)

substrate: benzil


substrate_name: benzylacetone

substrate: benzylacetone


substrate_name: propiophenone

substrate: propiophenone


substrate_name: menadione

substrate: menadione


substrate_name: p-nitrobenzaldehyde

substrate: p-nitrobenzaldehyde


substrate_name: Phenylglyoxal

substrate: Phenylglyoxal


substrate_name: p-quinone

substrate: p-quinone


substrate_name: acetophenone

substrate: acetophenone


substrate_name: methylglyoxal

smiles: CC(=O)C=O

substrate: methylglyoxal


smiles: CC(=O)CC(=O)C


substrate_name: acetoin

smiles: CC(C(=O)C)O

substrate: acetoin


substrate_name: pyruvoyltetrahydropterin

substrate: pyruvoyltetrahydropterin


substrate_name: sepiapterin

substrate: sepiapterin


substrate_name: diacetyl

smiles: CC(=O)C(=O)C

assay_conditions_summary: pH 6.4; 298.15 K; cofactor NADH

proof_lines:
pH 6.4, 25°C
cosubstrate NADH

substrate: diacetyl


substrate_name: NADPH

substrate: NADPH


enzyme_display_name: D-xylose 1-dehydrogenase (NADP(+), D-xylono-1,5-lactone-forming)

organism: Haloferax volcanii

substrate_name: NADP+

assay_conditions_summary: pH 8.3; 315.15 K; cofactor NADP+

substrate: NADP+


substrate_name: NAD+

enzyme: D-xylose 1-dehydrogenase (NADP(+), D-xylono-1,5-lactone-forming)

substrate: NAD+


substrate_name: D-xylose

substrate: D-xylose


substrate_name: D-glucose

substrate: D-glucose


enzyme_display_name: cinnamyl-alcohol dehydrogenase

organism: Leucaena leucocephala

substrate_name: cinnamaldehyde

enzyme: cinnamyl-alcohol dehydrogenase

substrate: cinnamaldehyde


substrate_name: cinnamyl alcohol

substrate: cinnamyl alcohol


substrate_name: coniferyl alcohol

substrate: coniferyl alcohol


assay_conditions_summary: pH 8.8; 313.15 K; cofactor NADP+


substrate_name: sinapyl alcohol

substrate: sinapyl alcohol


assay_conditions_summary: pH 6.5; 313.15 K; cofactor NADPH


substrate_name: coniferyl aldehyde

substrate: coniferyl aldehyde


substrate_name: p-coumaryl aldehyde

substrate: p-coumaryl aldehyde


substrate_name: sinapyl aldehyde

substrate: sinapyl aldehyde


enzyme_display_name: IMP dehydrogenase

organism: Borreliella burgdorferi

substrate_name: inosine 5'-phosphate

enzyme: IMP dehydrogenase

substrate: inosine 5'-phosphate


substrate_name: Unknown

substrate: Unknown


organism: Cryptosporidium parvum

substrate_name: 3-acetylpyridine adenine dinucleotide

proof_lines:
absence of K+, pH 8.0, 25°C
presence of 100 mM K+, pH 8.0, 25°C

substrate: 3-acetylpyridine adenine dinucleotide


assay_conditions_summary: pH 8; 298.15 K; cofactor NAD+

proof_lines:
absence of K+, pH 8.0, 25°C
presence of 1 mM K+, pH 8.0, 25°C
at pH 8.0 and 25°C


substrate_name: IMP

substrate: IMP


proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Zymomonas mobilis

substrate_name: 1-deoxy-D-xylulose 5-phosphate

smiles: CC(=O)C(C(COP(=O)(O)O)O)O

substrate: 1-deoxy-D-xylulose 5-phosphate


enzyme_display_name: diacetyl reductase [(S)-acetoin forming]

organism: Mycobacterium sp.

substrate_name: NADH

assay_conditions_summary: pH 7; 303.15 K; cofactor NADH

substrate: NADH


substrate_name: diacetyl

enzyme: diacetyl reductase [(S)-acetoin forming]

substrate: diacetyl


organism: Rhodococcus erythropolis


enzyme_display_name: 3alpha-hydroxysteroid 3-dehydrogenase

organism: Homo sapiens

proof_lines:
recombinant enzyme, at pH 7.4 and 25°C
native enzyme, at pH 7.4 and 25°C

enzyme: 3alpha-hydroxysteroid 3-dehydrogenase


proof_lines:
native enzyme, at pH 7.4 and 25°C
recombinant enzyme, at pH 7.4 and 25°C


substrate_name: 9alpha,11beta-prostaglandin F2

substrate: 9alpha,11beta-prostaglandin F2


proof_lines: pH 7.4, 37°C, recombinant wild-type enzyme


proof_lines: mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C


substrate_name: NAD+

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NAD+

proof_lines: wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C

substrate: NAD+


substrate_name: cholic acid

substrate: cholic acid


substrate_name: NADP+

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADP+

substrate: NADP+


proof_lines: mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C


substrate_name: (S)-tetralol

substrate: (S)-tetralol


substrate_name: deoxycholic acid

substrate: deoxycholic acid


substrate_name: lithocholic acid

substrate: lithocholic acid


substrate_name: chenodeoxycholic acid

substrate: chenodeoxycholic acid


substrate_name: androsterone

substrate: androsterone


substrate_name: (R)-tetralol

substrate: (R)-tetralol


substrate_name: dihydrotestosterone

proof_lines:
pH 7.4, 37°C
pH 7.5, 37°C
apparent Km-value, pH 7.4, 37°C

substrate: dihydrotestosterone


substrate_name: testosterone

substrate: testosterone


enzyme_display_name: (S)-malate dehydrogenase (NAD(+), oxaloacetate-forming)

organism: Comamonas testosteroni


substrate_name: oxaloacetate

enzyme: (S)-malate dehydrogenase (NAD(+), oxaloacetate-forming)

substrate: oxaloacetate


substrate_name: L-malate

proof_lines:
0.03-0.1 mM substrate
0.2-10 mM substrate

substrate: L-malate


substrate_name: deamino-NAD+

substrate: deamino-NAD+


organism: Methanospirillum hungatei


substrate_name: NADH

substrate: NADH


organism: Phycomyces blakesleeanus


enzyme_display_name: UDP-N-acetylglucosamine 3-dehydrogenase

organism: Methanococcus maripaludis

substrate_name: UDP-N-acetyl-alpha-D-glucosamine

enzyme: UDP-N-acetylglucosamine 3-dehydrogenase

substrate: UDP-N-acetyl-alpha-D-glucosamine


assay_conditions_summary: pH 8.5; 310.15 K; cofactor NAD+


assay_conditions_summary: pH 7.5; 315.15 K; cofactor NAD+


substrate_name: D-glucose 6-phosphate

smiles: C(C(C(C(C(C=O)O)O)O)O)OP(=O)(O)O

substrate: D-glucose 6-phosphate


enzyme_display_name: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+))

organism: Azospirillum brasilense

substrate_name: pyruvate

substrate: pyruvate


substrate_name: (S)-malate

assay_conditions_summary: pH 8; 303.15 K; cofactor NADP+

proof_lines: in the presence of 1 mM NADP+, at pH 8.0 and 30°C

enzyme: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+))

substrate: (S)-malate


substrate_name: NADP+

substrate: NADP+


substrate_name: Unknown

assay_conditions_summary: pH 8; 303.15 K; cofactor CoA

substrate: Unknown


assay_conditions_summary: pH 7.4; 303.15 K; cofactor NADP+


substrate_name: NAD+

assay_conditions_summary: pH 7.4; 303.15 K; cofactor NAD+

substrate: NAD+


substrate_name: NADPH

assay_conditions_summary: pH 7.4; 303.15 K; cofactor NADPH

substrate: NADPH


proof_lines: mutant enzyme H51A/D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C


proof_lines: mutant enzyme D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C


proof_lines: mutant enzyme H142A/D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C


proof_lines: mutant enzyme D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C


assay_conditions_summary: pH 7.4; cofactor NAD+


assay_conditions_summary: pH 7.4; cofactor NADP+


assay_conditions_summary: pH 7.4; cofactor ATP

proof_lines: in the presence of NAD+, in 50 mM Tris-HCl (pH 7.4)

proof_lines: in the presence of NADP+, in 50 mM Tris-HCl (pH 7.4)


proof_lines: wild type enzyme, in 50 mMTris-HCl (pH 7.4), at 30°C


proof_lines: wild-type enzyme, in presence of NADP+, pH 7.4, 30°C

proof_lines: wild-type enzyme, in presence of NAD+, pH 7.4, 30°C


assay_conditions_summary: cofactor NADP+

proof_lines:
with NADP+ as cosubstrate
cytosolic enzyme
with NAD+ as cosubstrate


organism: Ricinus communis

assay_conditions_summary: pH 7.6; 298.15 K; cofactor NADP+


assay_conditions_summary: pH 7.6; 298.15 K; cofactor NADPH

proof_lines:
versus NADP+, pH 7.6-7.7, 25°C
versus L-malate, pH 7.6-7.7, 25°C


enzyme_display_name: isocitrate dehydrogenase (NAD(+))

organism: Yarrowia lipolytica

enzyme: isocitrate dehydrogenase (NAD(+))


substrate_name: isocitrate

substrate: isocitrate


enzyme_display_name: isocitrate dehydrogenase (NADP(+))

organism: Chlorobium limicola

assay_conditions_summary: pH 8

proof_lines:
pH 8.0, forward reaction
recombinant enyme, pH 9.0

enzyme: isocitrate dehydrogenase (NADP(+))


assay_conditions_summary: pH 9; cofactor NADP+


proof_lines:
pH 6.5, reverse reaction
recombinant enyme, pH 7.0


assay_conditions_summary: pH 7

proof_lines:
recombinant enyme, pH 7.0
pH 6.5, reverse reaction


assay_conditions_summary: pH 6.5; cofactor NADPH


proof_lines:
recombinant, renatured enzyme, pH 8.0
pH 8.0, 30°C


substrate_name: DL-isocitrate

substrate: DL-isocitrate


organism: Rattus norvegicus

enzyme: phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating)


proof_lines: pH 7.6, and temperature not specified in the publication, noncompetitive inhibition with respect to NADP+

proof_lines: pH 7.6, and temperature not specified in the publication, competitive inhibition with respect to 6-phospho-D-gluconate


organism: Trypanosoma brucei


proof_lines: in the presence of D-glucose 6-phosphate


organism: Sus scrofa

substrate_name: progesterone

substrate: progesterone


substrate_name: beta-NADPH

assay_conditions_summary: cofactor NADPH

substrate: beta-NADPH


substrate_name: beta-NADP+

substrate: beta-NADP+


substrate_name: beta-NAD+

assay_conditions_summary: cofactor NAD+

substrate: beta-NAD+


substrate_name: alpha-NADH

assay_conditions_summary: cofactor NADH

substrate: alpha-NADH


substrate_name: pregnenolone

substrate: pregnenolone


substrate_name: deoxycorticosterone

substrate: deoxycorticosterone


enzyme_display_name: 4-hydroxybutyrate dehydrogenase

organism: Cupriavidus necator

substrate_name: gamma-hydroxybutyrate

smiles: C(CC(=O)O)CO

enzyme: 4-hydroxybutyrate dehydrogenase

substrate: gamma-hydroxybutyrate


substrate_name: ethanol

smiles: CCO

substrate: ethanol


enzyme_display_name: malate dehydrogenase (NADP(+))

organism: Lathyrus oleraceus

substrate_name: oxaloacetate

proof_lines: mutant enzyme C23A/C28A/C364A/C376A, oxidized form

enzyme: malate dehydrogenase (NADP(+))

substrate: oxaloacetate


proof_lines: mutant enzyme C23A/C28A and C23A/C28A/C376A, oxidized form


proof_lines: mutant enzyme C23A/C28A/C206A/C364A, oxidized form


proof_lines: mutant enzyme C23A/C28A/C206A/C376A, oxidized form

proof_lines: mutant enzyme C23A/C28A/C206A, reduced enzyme form and mutant enzyme C23A/C28A/C376A, oxidized form


proof_lines: mutant enzyme C23A/C28A/C206A/C364A, oxidized form and mutant DELTAN, lacking the 45 N-terminal residues


proof_lines: mutant enzyme C23A/C28A/C364A and C23A/C28A/C364A/C376A, oxidized form


proof_lines: wild-type enzyme, reduced form and mutant enzyme C23A/C28A/C364A, oxidized form


proof_lines: wild-type enzyme and mutant C28A, reduced enzyme form


proof_lines: mutant enzyme lacking the N-terminal 45 residues, reduced form

proof_lines: mutant enzyme lacking the N-terminal 45 residues, oxidized form


proof_lines: mutant enzyme C23A/C28A/C206A, oxidized form and mutant DELTAN lacking the N-terminal 45 residues, reduced form

proof_lines: mutant enzyme C23A/C28A/C206A, reduced form


proof_lines:
reduced native enzyme
reduced enzyme, + 200 mM guanidine
oxidized enzyme, + 250 mM guanidine


proof_lines: mutant enzyme C23A/C28A/C206A, oxidized form


mutation_signature: reduced truncated enzyme reduced truncated enzyme


mutation_signature: oxidized truncated enzyme oxidized truncated enzyme


enzyme_display_name: 3-isopropylmalate dehydrogenase

organism: Acidithiobacillus ferrooxidans

assay_conditions_summary: pH 9; cofactor NAD+

enzyme: 3-isopropylmalate dehydrogenase


substrate_name: L-malate

assay_conditions_summary: pH 9

substrate: L-malate


substrate_name: malate

substrate: malate


smiles: CC(C(C(=O)O)O)C(=O)O


smiles: CCC(C(C(=O)O)O)C(=O)O


enzyme_display_name: homoisocitrate dehydrogenase

organism: Candida albicans

proof_lines: recombinant His-tagged enzyme, pH 7.8, 20°C

enzyme: homoisocitrate dehydrogenase


assay_conditions_summary: pH 7.8; 293.15 K; cofactor NAD+


enzyme_display_name: aryl-alcohol dehydrogenase

organism: Acinetobacter baylyi

substrate_name: NADH

assay_conditions_summary: pH 7.4; 303.15 K; cofactor NADH

enzyme: aryl-alcohol dehydrogenase

substrate: NADH


substrate_name: benzaldehyde

substrate: benzaldehyde


enzyme_display_name: aryl-alcohol dehydrogenase (NADP(+))

organism: Ralstonia sp.

substrate_name: NADP+

assay_conditions_summary: pH 8.3; 303.15 K; cofactor NADP+

proof_lines: mutant N15G/G37D/R38V/R39S, pH 8.3, 30°C

enzyme: aryl-alcohol dehydrogenase (NADP(+))

substrate: NADP+


substrate_name: NAD+

assay_conditions_summary: pH 8.3; 303.15 K; cofactor NAD+

substrate: NAD+


enzyme_display_name: phosphoglycerate dehydrogenase

organism: Corynebacterium glutamicum

assay_conditions_summary: pH 7; buffer 50 mM MOPS

proof_lines: in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication

proof_lines: in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication

enzyme: phosphoglycerate dehydrogenase


substrate_name: Unknown

substrate: Unknown


enzyme_display_name: D-lactate dehydrogenase (cytochrome)

organism: Oryza sativa Indica Group

substrate_name: (R)-lactate

proof_lines: cosubstrate: phenazine methosulfate and 2,6-dichlorophenolindophenol, pH 8.35, 25°C

proof_lines: cosubstrate: ferrocytochrome c, pH 8.35, 25°C

enzyme: D-lactate dehydrogenase (cytochrome)

substrate: (R)-lactate


enzyme_display_name: (S)-2-hydroxy-acid oxidase

substrate_name: L-2-hydroxy palmitate

enzyme: (S)-2-hydroxy-acid oxidase

substrate: L-2-hydroxy palmitate


substrate_name: (S)-lactate

substrate: (S)-lactate


organism: Rattus sp.

substrate_name: Bromopyruvate

smiles: C(C(=O)C(=O)O)Br

substrate: Bromopyruvate


smiles: CCCCC(C(=O)O)O


substrate_name: DL-methionine

smiles: CSCCC(C(=O)O)N

substrate: DL-methionine


substrate_name: L-tryptophan

substrate: L-tryptophan


substrate_name: glycolate

smiles: C(C(=O)O)O

substrate: glycolate


substrate_name: glyoxylate

substrate: glyoxylate


smiles: CC(C)C(C(=O)O)O


substrate_name: L-lysine

smiles: C(CCN)CC(C(=O)O)N

substrate: L-lysine


smiles: O=O

proof_lines: oxidation of propane-1,3-dithiol-glyoxylate adduct


enzyme_display_name: glycerol-3-phosphate oxidase

organism: Aerococcus viridans

substrate_name: sn-glycerol 3-phosphate

smiles: C(C(COP(=O)(O)O)O)O

enzyme: glycerol-3-phosphate oxidase

substrate: sn-glycerol 3-phosphate


enzyme_display_name: 5-(hydroxymethyl)furfural oxidase

organism: Pseudomonas sp. 11/12A

enzyme: 5-(hydroxymethyl)furfural oxidase


enzyme_display_name: galactose oxidase

organism: Fusarium graminearum

substrate_name: D-galactose

enzyme: galactose oxidase

substrate: D-galactose


smiles: C=C(CO)CO


proof_lines: pH 7.0, 30°C, recombinant wild-type enzyme


substrate_name: galactoglucomannan

proof_lines: pH 7.0, 25°C, recombinant wild-type enzyme

substrate: galactoglucomannan


proof_lines: pH 7.0, 25°C, recombinant chimeric enzyme mutant GaO-CBM29

proof_lines: pH 7.0, 25°C, recombinant chimeric enzyme mutant CBM29-GaO


proof_lines: pH 7.0, 30°C, recombinant chimeric enzyme mutant CBM29-GaO

proof_lines: pH 7.0, 30°C, recombinant chimeric enzyme mutant GaO-CBM29


organism: Fusarium oxysporum


enzyme_display_name: L-sorbose 1-dehydrogenase

organism: Sinorhizobium sp.

substrate_name: L-sorbose

enzyme: L-sorbose 1-dehydrogenase

substrate: L-sorbose


enzyme_display_name: hydrogen dehydrogenase

smiles: [HH]

enzyme: hydrogen dehydrogenase


assay_conditions_summary: pH 8; cofactor NAD+

proof_lines: pH 8.0, temperature not specified in the publication


substrate_name: NADH

assay_conditions_summary: pH 8; cofactor NADH

proof_lines:
electron acceptor resazurine
electron acceptor benzyl viologen
electron acceptor methyl viologen

substrate: NADH


organism: Rhodococcus opacus


enzyme_display_name: hydrogen dehydrogenase (NADP(+))

organism: Solidesulfovibrio fructosivorans

substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

enzyme: hydrogen dehydrogenase (NADP(+))

substrate: NADP+


enzyme_display_name: ferredoxin hydrogenase

organism: Clostridium pasteurianum

substrate_name: reduced methyl viologen

enzyme: ferredoxin hydrogenase

substrate: reduced methyl viologen


substrate_name: reduced ferredoxin

proof_lines:
hydrogen evolution, hydrogenase I
hydrogenase I, at pH 8.0 and 30°C
hydrogen evolution

substrate: reduced ferredoxin


enzyme_display_name: sulfhydrogenase

organism: Pyrococcus furiosus

assay_conditions_summary: pH 8; 353.15 K; cofactor NADP+

enzyme: sulfhydrogenase


substrate_name: NADPH

assay_conditions_summary: pH 8; 353.15 K; cofactor NADPH

substrate: NADPH


assay_conditions_summary: pH 8; 353.15 K; cofactor NADH


substrate_name: methyl viologen

substrate: methyl viologen


enzyme_display_name: protocatechuate 3,4-dioxygenase

organism: Acinetobacter calcoaceticus

enzyme: protocatechuate 3,4-dioxygenase


substrate_name: protocatechuate

substrate: protocatechuate


substrate_name: Unknown

substrate: Unknown


organism: Rhizobium sp. LMB-1

proof_lines: Fe3O4 nanoparticles-immobilized enzyme, at pH 7.4 and 37°C


enzyme_display_name: homogentisate 1,2-dioxygenase

organism: Aspergillus nidulans

substrate_name: homogentisate

enzyme: homogentisate 1,2-dioxygenase

substrate: homogentisate


enzyme_display_name: indoleamine 2,3-dioxygenase

organism: Rattus norvegicus

substrate_name: L-Trp

assay_conditions_summary: pH 8

enzyme: indoleamine 2,3-dioxygenase

substrate: L-Trp


enzyme_display_name: gibberellin 3beta-dioxygenase

organism: Phaseolus vulgaris

enzyme: gibberellin 3beta-dioxygenase


enzyme_display_name: benzoate 1,2-dioxygenase

organism: Rhodococcus ruber

substrate_name: benzoate

enzyme: benzoate 1,2-dioxygenase

substrate: benzoate


enzyme_display_name: trimethylamine monooxygenase

organism: Aminobacter aminovorans

assay_conditions_summary: cofactor NADPH

proof_lines: pH and temperature not specified in the publication

enzyme: trimethylamine monooxygenase


enzyme_display_name: magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase

organism: Rubrivivax gelatinosus

substrate_name: magnesium-protoporphyrin IX 13-monomethyl ester

proof_lines: Michaelis-Menten kinetics, recombinant enzyme, pH 7.7, 30°C

proof_lines: sigmoidal kinetics, recombinant enzyme, pH 7.7, 30°C


enzyme_display_name: styrene monooxygenase

organism: Pseudomonas putida

substrate_name: Styrene

enzyme: styrene monooxygenase

substrate: Styrene


enzyme_display_name: deoxyhypusine monooxygenase

substrate_name: deoxyhypusine

smiles: C(CCNCCCCN)CC(C(=O)O)N

enzyme: deoxyhypusine monooxygenase

substrate: deoxyhypusine


enzyme_display_name: xanthine oxidase

organism: Gallus gallus

substrate_name: xanthine

proof_lines:
kidney enzyme
liver enzyme

enzyme: xanthine oxidase

substrate: xanthine


organism: Anabaena sp.

substrate_name: CTP

substrate: CTP


enzyme_display_name: aspartate-semialdehyde dehydrogenase

organism: Blastomyces dermatitidis

enzyme: aspartate-semialdehyde dehydrogenase


organism: Huso huso

assay_conditions_summary: cofactor NAD+


substrate_name: NAD+

substrate: NAD+


organism: Mycobacterium tuberculosis

substrate_name: phosphate

proof_lines: pH 8.0, temperature not specified in the publication, recombinant His-tagged wild-type enzyme

substrate: phosphate


proof_lines: versus NAD+, pH 8.0, temperature not specified in the publication, recombinant His-tagged wild-type enzyme

proof_lines: versus D-glyceraldehyde 3-phosphate, pH 8.0, temperature not specified in the publication, recombinant His-tagged wild-type enzyme

proof_lines: versus arsenate, pH 8.0, temperature not specified in the publication, recombinant His-tagged wild-type enzyme


substrate_name: arsenate

substrate: arsenate


organism: Streptomyces arenae

substrate_name: D-glyceraldehyde 3-phosphate

smiles: C(C(C=O)O)OP(=O)(O)O

proof_lines:
pentalenolactone-insensitive enzyme
pentalenolactone-sensitive enzyme

substrate: D-glyceraldehyde 3-phosphate


proof_lines:
pentalenolactone-sensitive enzyme
pentalenolactone-insensitive enzyme


enzyme_display_name: glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating)

organism: Chlamydomonas reinhardtii

proof_lines: wild type enzyme, pH and temperature not specified in the publication


substrate_name: 3-phospho-D-glyceroyl phosphate

assay_conditions_summary: pH 7.7; 303.15 K; cofactor NADPH

proof_lines: pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase, reaction with NADH

proof_lines: pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase, reaction with NADH

proof_lines: pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase, reaction with NADPH

enzyme: glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating)

substrate: 3-phospho-D-glyceroyl phosphate


substrate_name: NADH

assay_conditions_summary: pH 7.7; 303.15 K; cofactor NADH

proof_lines: pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase

proof_lines: pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase

substrate: NADH


substrate_name: NADPH

substrate: NADPH


assay_conditions_summary: cofactor NADH


proof_lines: mutant enzyme R82A, pH and temperature not specified in the publication


proof_lines: mutant enzyme R82D, pH and temperature not specified in the publication


organism: Spinacia oleracea


proof_lines: with NADH as coenzyme, activation by 20 mM dithiothreitol and 0.021 mM 1,3-bisphosphoglycerate


proof_lines: AB-GAPDH, analyzed under reducing conditions

proof_lines: AB-GAPDH, analyzed under oxidizing conditions


proof_lines: mutant enzyme T33A/S188A, reaction with NADH

proof_lines: mutant enzyme T33A/S188A, reaction with NADPH


proof_lines: native enzyme A3-GAPDH, reaction with NADH

proof_lines: native enzyme A3-GAPDH, reaction with NADPH

proof_lines: with NADH as cofactor, activation by 20 mM dithiothreitol and 0.021 mM 1,3-bisphosphoglycerate


proof_lines:
mutant enzyme T33A, reaction with NADH
mutant enzyme T33A, reaction with NADPH


proof_lines: mutant enzyme S188A, reaction with NADPH


proof_lines: mutant B(188)A, analyzed under oxidizing conditions

proof_lines: A(plusCTE) mutant, analyzed under oxidizing conditions


proof_lines: A(plusCTE) mutant, analyzed under reducing conditions


organism: Cereibacter sphaeroides

substrate_name: NADP+

substrate: NADP+


substrate_name: succinate semialdehyde

smiles: C(CC(=O)O)C=O

substrate: succinate semialdehyde


enzyme_display_name: 2,5-dioxovalerate dehydrogenase

organism: Bacillus subtilis

enzyme: 2,5-dioxovalerate dehydrogenase


substrate_name: heptaldehyde

smiles: CCCCCCC=O

substrate: heptaldehyde


substrate_name: Octylaldehyde

smiles: CCCCCCCC=O

substrate: Octylaldehyde


substrate_name: alpha-Ketoglutaric semialdehyde

proof_lines:
with NAD+ as cofactor
with NADP+ as cofactor

substrate: alpha-Ketoglutaric semialdehyde


substrate_name: propionaldehyde

smiles: CCC=O

substrate: propionaldehyde


substrate_name: butylaldehyde

smiles: CCCC=O

substrate: butylaldehyde


substrate_name: hexylaldehyde

smiles: CCCCCC=O

substrate: hexylaldehyde


substrate_name: Valeraldehyde

smiles: CCCCC=O

substrate: Valeraldehyde


enzyme_display_name: aldehyde dehydrogenase (NAD(+))

organism: Geobacillus thermodenitrificans

substrate_name: acetaldehyde

smiles: CC=O

assay_conditions_summary: pH 8; 333.15 K; buffer 50 mM KH2PO4-K2HPO4

proof_lines: in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60°C

enzyme: aldehyde dehydrogenase (NAD(+))

substrate: acetaldehyde


substrate_name: NAD+

substrate: NAD+


organism: Penicillium chrysogenum

smiles: C(CC(C(=O)O)N)CC(=O)O


assay_conditions_summary: pH 8; 303.15 K; cofactor NADPH


substrate_name: ATP

assay_conditions_summary: pH 8; 303.15 K; cofactor ATP

substrate: ATP


enzyme_display_name: retinal dehydrogenase

organism: Homo sapiens

substrate_name: all-trans-retinal

enzyme: retinal dehydrogenase

substrate: all-trans-retinal


substrate_name: decanal

smiles: CCCCCCCCCC=O

substrate: decanal


substrate_name: all-trans-retinol

substrate: all-trans-retinol


substrate_name: NADH

substrate: NADH


substrate_name: hexanal

substrate: hexanal


substrate_name: Unknown

substrate: Unknown


substrate_name: benzaldehyde

substrate: benzaldehyde


assay_conditions_summary: pH 7.1; cofactor NAD+

proof_lines: partially purified recombinant ALDH1A1 at pH 7.1 and in the presence of 0.1 mM 3-deoxyglucosone


smiles: C(C(C(CO)O)O)C(=O)C=O

proof_lines: partially purified recombinant ALDH1A1 at pH 7.1 and in the presence of 1 mM NAD


organism: Mus musculus

proof_lines: recombinant wild-type enzyme, pH 8.5, 25°C


proof_lines: recombinant wild-type enzyme and mutant L459F/N460G, pH 8.5, 25°C


assay_conditions_summary: pH 8.5; 298.15 K; cofactor NAD+


substrate_name: octanal

substrate: octanal


substrate_name: retinal

proof_lines:
all-trans-retinal, wild-type
wild-type

substrate: retinal


proof_lines:
all-trans-retinal, F471L mutant
F471L mutant


enzyme_display_name: cinnamoyl-CoA reductase

organism: Leucaena leucocephala

substrate_name: feruloyl-CoA

assay_conditions_summary: cofactor CoA

proof_lines: mutant enzyme H215L, pH and temperature not specified in the publication

enzyme: cinnamoyl-CoA reductase

substrate: feruloyl-CoA


proof_lines: mutant enzyme R51G/D77G, pH and temperature not specified in the publication


proof_lines: mutant enzyme F30V, pH and temperature not specified in the publication


proof_lines: mutant enzyme RF30V/I31N, pH and temperature not specified in the publication


proof_lines: mutant enzyme S99G, pH and temperature not specified in the publication


proof_lines: mutant enzyme I31N, pH and temperature not specified in the publication


proof_lines: mutant enzyme L64W, pH and temperature not specified in the publication


proof_lines: wild type enzyme, pH and temperature not specified in the publication


substrate_name: sinapaldehyde

proof_lines: in 100 mM phosphate buffer pH 6.5, at 30°C

proof_lines: in 100 mM phosphate buffer pH 7.8, at 30°C

substrate: sinapaldehyde


substrate_name: coniferaldehyde

substrate: coniferaldehyde


substrate_name: coumaroyl-CoA

assay_conditions_summary: pH 6.5; 303.15 K; buffer pH 6; cofactor CoA

substrate: coumaroyl-CoA


substrate_name: caffeoyl-CoA

substrate: caffeoyl-CoA


substrate_name: sinapoyl-CoA

substrate: sinapoyl-CoA


enzyme_display_name: aldehyde dehydrogenase [NAD(P)(+)]

substrate_name: NAD+

enzyme: aldehyde dehydrogenase [NAD(P)(+)]

substrate: NAD+


substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

substrate: NADP+


substrate_name: acetaldehyde

substrate: acetaldehyde


organism: Rattus norvegicus

proof_lines:
enzyme I
enzyme III


proof_lines:
enzyme I
enzyme II
enzyme III


proof_lines:
enzyme I
enzyme III
enzyme II


proof_lines: enzyme from HTC hepatoma cells, reaction with NADP+


substrate_name: propanal

proof_lines: enzyme from HTC hepatoma cells, reaction with NAD+

substrate: propanal


proof_lines: enzyme from cornea, reaction with propanal

proof_lines: enzyme from HTC hepatoma cells, reaction with propanal


proof_lines:
with respect to NADP+
with respect to NAD+


proof_lines: enzyme from HTC hepatoma cells, reaction with benzaldehyde

proof_lines: enzyme from cornea, reaction with benzaldehyde


enzyme_display_name: malonyl CoA reductase (malonate semialdehyde-forming)

organism: Metallosphaera sedula

substrate_name: malonyl-CoA

assay_conditions_summary: pH 7.8; 338.15 K; cofactor CoA

substrate: malonyl-CoA


organism: Sulfurisphaera tokodaii

proof_lines:
per subunit, pH 7.8, 65°C
pH 7.8, 65°C

enzyme: malonyl CoA reductase (malonate semialdehyde-forming)


substrate_name: NADPH

assay_conditions_summary: pH 7.8; 338.15 K; cofactor NADPH

substrate: NADPH


organism: Pseudomonas putida

substrate_name: succinate semialdehyde

substrate: succinate semialdehyde


assay_conditions_summary: pH 8; 303.15 K; cofactor NADP+


enzyme_display_name: L-2-aminoadipate reductase

proof_lines: pH not specified in the publication, temperature not specified in the publication

enzyme: L-2-aminoadipate reductase


assay_conditions_summary: pH 9; 303.15 K; cofactor NADPH


substrate_name: ATP

assay_conditions_summary: pH 9; 303.15 K; cofactor ATP

substrate: ATP


substrate_name: Unknown

substrate: Unknown


enzyme_display_name: oxalate oxidase

organism: Gelatoporia subvermispora

substrate_name: oxalate

enzyme: oxalate oxidase

substrate: oxalate


proof_lines: mutant enzyme D241A, pH and temperature not specified in the publication


assay_conditions_summary: pH 4; 298.15 K; buffer citrate

proof_lines:
succinate buffer, at pH 4.0 and 25°C
in citrate buffer, at pH 4.0 and 22°C
citrate buffer, at pH 4.0 and 25°C


proof_lines:
at pH 4.0 and 25°C
in citrate buffer, at pH 4.0 and 22°C


substrate_name: oxalic acid

assay_conditions_summary: pH 4

substrate: oxalic acid


enzyme_display_name: pyruvate synthase

organism: Pseudothermotoga hypogea

substrate_name: CoA

assay_conditions_summary: pH 8.4; 353.15 K; cofactor CoA

enzyme: pyruvate synthase

substrate: CoA


substrate_name: pyruvate

substrate: pyruvate


substrate_name: oxidized methyl viologen

substrate: oxidized methyl viologen


enzyme_display_name: oxalate oxidoreductase

organism: Neomoorella thermoacetica

proof_lines: in 50 mM Tris-HCl, 2 mM dithiothreitol, pH 7.9, at 25°C

enzyme: oxalate oxidoreductase


enzyme_display_name: anaerobic carbon-monoxide dehydrogenase

organism: Carboxydothermus hydrogenoformans

substrate_name: CO

smiles: [Co]

proof_lines:
at 20°C
at 70°C

enzyme: anaerobic carbon-monoxide dehydrogenase

substrate: CO


substrate_name: methyl viologen

substrate: methyl viologen


proof_lines: at 23°C, in 4 mM dithionite, and 4 mM dithiothreitol


organism: Methanothermobacter thermautotrophicus


substrate_name: Uracil

substrate: Uracil


assay_conditions_summary: cofactor NADPH

proof_lines:
in presence of uracil
in presence of thymine
in presence of 5-fluorouracil


substrate_name: thymine

substrate: thymine


substrate_name: NADP+

proof_lines:
in presence of dihydrouracil
in presence of dihydrothymine

substrate: NADP+


substrate_name: Dihydrothymine

substrate: Dihydrothymine


substrate_name: dihydrouracil

substrate: dihydrouracil


substrate_name: trans-benzene dihydrodiol

substrate: trans-benzene dihydrodiol


substrate_name: cholic acid

substrate: cholic acid


substrate_name: androsterone

substrate: androsterone


substrate_name: lithocholic acid

substrate: lithocholic acid


enzyme_display_name: maleylacetate reductase

organism: Sphingobium chlorophenolicum

substrate_name: NADPH

assay_conditions_summary: pH 7; 296.15 K; cofactor NADPH

proof_lines: cosubstrate: 2-maleylacetate, pH 7.0, 23°C

proof_lines: cosubstrate: 2-chloromaleylacetate, pH 7.0, 23°C

enzyme: maleylacetate reductase

substrate: NADPH


proof_lines: pH 7.0, 23°C, wild-type enzyme, cosubstrate: 2-maleylacetate

proof_lines: pH 7.0, 23°C, wild-type enzyme, cosubstrate: 2-chloromaleylacetate


substrate_name: NADH

assay_conditions_summary: pH 7; 296.15 K; cofactor NADH

substrate: NADH


proof_lines: pH 7.0, 23°C, wild-type enzyme, cosubstrate: NADH


substrate_name: maleylacetate

assay_conditions_summary: pH 7

proof_lines:
at pH 7.0 in 20 mM Tris-HCl
at pH 7.0

substrate: maleylacetate


enzyme_display_name: protochlorophyllide reductase

organism: Nostoc punctiforme

proof_lines: pH and temperature not specified in the publication

enzyme: protochlorophyllide reductase


substrate_name: protochlorophyllide

substrate: protochlorophyllide


enzyme_display_name: acrylyl-CoA reductase (NADPH)

organism: Escherichia coli

substrate_name: acrylyl-CoA

assay_conditions_summary: pH 7; 303.15 K; cofactor NADPH

proof_lines: pH 7.0, 30°C, recombinant His-tagged enzyme

enzyme: acrylyl-CoA reductase (NADPH)

substrate: acrylyl-CoA


enzyme_display_name: crotonyl-CoA carboxylase/reductase

organism: Cereibacter sphaeroides

proof_lines: at pH 7.8, temperature not specified in the publication

enzyme: crotonyl-CoA carboxylase/reductase


substrate_name: (E)-crotonyl-CoA

proof_lines: for reduction, pH and temperature not specified in the publication

proof_lines: for reductive carboxylation, pH and temperature not specified in the publication

substrate: (E)-crotonyl-CoA


substrate_name: acryloyl-CoA

substrate: acryloyl-CoA


organism: Cereibacter sphaeroides 2.4.1

assay_conditions_summary: pH 7.9; buffer 100 mM Tris-HCl; cofactor CoA

proof_lines: in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication


substrate_name: crotonyl-CoA

substrate: crotonyl-CoA


assay_conditions_summary: pH 7.9; buffer 100 mM Tris-HCl; cofactor NADPH


organism: Alistipes finegoldii

assay_conditions_summary: pH 8; 303.15 K; cofactor NADH

proof_lines: K0.5 value, Hill coefficient 2.4, pH 8, 30°C


substrate_name: crotonoyl-CoA

assay_conditions_summary: pH 8; 303.15 K; cofactor CoA

proof_lines: K0.5 value, Hill coefficient 1.5, pH 8, 30°C

substrate: crotonoyl-CoA


enzyme_display_name: coproporphyrinogen oxidase

substrate_name: Coproporphyrinogen-III

enzyme: coproporphyrinogen oxidase

substrate: Coproporphyrinogen-III


enzyme_display_name: protoporphyrinogen oxidase

organism: Bos taurus

substrate_name: protoporphyrinogen-IX

enzyme: protoporphyrinogen oxidase

substrate: protoporphyrinogen-IX


enzyme_display_name: acyl-CoA oxidase

organism: Beauveria bassiana

substrate_name: lignoceroyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: acyl-CoA oxidase

substrate: lignoceroyl-CoA


enzyme_display_name: succinate dehydrogenase

organism: Plasmodium yoelii yoelii

proof_lines: differences in Km value (9fold) and Vmax/Km ratio (19fold) between Q1 and Q2 indicate that the 6-polyprenyl tail of the ubiquinone ring contributes to the binding affinity and that Q2 is better substrate than Q1

enzyme: succinate dehydrogenase


substrate_name: succinate

substrate: succinate


organism: Rhodothermus marinus

proof_lines: reaction with phenazine methosulfate and 2,6-dichlorophenolindophenol


substrate_name: fumarate

substrate: fumarate


enzyme_display_name: 6-hydroxynicotinate reductase

organism: Clostridium sp.

substrate_name: reduced ferredoxin

enzyme: 6-hydroxynicotinate reductase

substrate: reduced ferredoxin


substrate_name: reduced methyl viologen

substrate: reduced methyl viologen


enzyme_display_name: isovaleryl-CoA dehydrogenase

organism: Rattus norvegicus

substrate_name: FAD

assay_conditions_summary: cofactor FAD

enzyme: isovaleryl-CoA dehydrogenase

substrate: FAD


substrate_name: phenazine methosulfate

substrate: phenazine methosulfate


substrate_name: n-valeryl-CoA

substrate: n-valeryl-CoA


substrate_name: Unknown

assay_conditions_summary: pH 8; 310.15 K; cofactor CoA

substrate: Unknown


substrate_name: isovaleryl-CoA

proof_lines: purified enzyme in the absence of electron transfer agents

substrate: isovaleryl-CoA


assay_conditions_summary: pH 7.4; 310.15 K; cofactor CoA


proof_lines: wild-type enzyme and mutant IVD G375E, pH 7.4, 37°C


enzyme_display_name: glutaryl-CoA dehydrogenase (ETF)

organism: Mus musculus

substrate_name: glutaryl-CoA

enzyme: glutaryl-CoA dehydrogenase (ETF)

substrate: glutaryl-CoA


proof_lines: wild-type enzyme, pH and temperature not specified in the publication


proof_lines: mutant E370Q, pH and temperature not specified in the publication


enzyme_display_name: 3-oxosteroid 1-dehydrogenase

organism: Sterolibacterium denitrificans

enzyme: 3-oxosteroid 1-dehydrogenase


proof_lines: pH 6.5, 30°C, recombinant enzyme, with 4-cholest-3-one

proof_lines: pH 6.5, 30°C, recombinant enzyme, with 4-androstene-3,17-dione


enzyme_display_name: alanine dehydrogenase

organism: Alkalihalophilus pseudofirmus

substrate_name: L-alanine

smiles: CC(C(=O)O)N

proof_lines: recombinant His-tagged mutant K73Q, pH 10.5, 40°C

enzyme: alanine dehydrogenase

substrate: L-alanine


substrate_name: NAD+

assay_conditions_summary: pH 10.5; 313.15 K; cofactor NAD+

substrate: NAD+


proof_lines: recombinant His-tagged mutant K73E, pH 10.5, 40°C


proof_lines: recombinant His-tagged mutant K73S, pH 10.5, 40°C


proof_lines: recombinant His-tagged mutant K73R, pH 10.5, 40°C


proof_lines: recombinant His-tagged mutant K73Y, pH 10.5, 40°C


proof_lines: recombinant His-tagged mutant K73F, pH 10.5, 40°C


proof_lines: recombinant His-tagged wild-type enzyme, pH 10.5, 40°C


proof_lines: recombinant His-tagged mutant K73A, pH 10.5, 40°C


proof_lines: recombinant His-tagged mutants K73R, K73S, and K73F, pH 10.5, 40°C


proof_lines: recombinant His-tagged mutant K73E and K73Y, pH 10.5, 40°C


enzyme_display_name: glutamate synthase (NADH)

organism: Phaseolus vulgaris

substrate_name: NADH

assay_conditions_summary: cofactor NADH

proof_lines:
NADH-GOGAT II
NADH-GOGAT I

enzyme: glutamate synthase (NADH)

substrate: NADH


proof_lines:
NADH-GOGAT I
NADH-GOGAT II


substrate_name: L-glutamine

smiles: C(CC(=O)N)C(C(=O)O)N

substrate: L-glutamine


enzyme_display_name: diaminopimelate dehydrogenase

organism: Corynebacterium glutamicum

proof_lines:
pH 8.5, 30°C
reductive amination
oxidative deamination at pH 7.8

enzyme: diaminopimelate dehydrogenase


smiles: C(CC(C(=O)O)N)CC(C(=O)O)N


substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

substrate: NADP+


substrate_name: meso-diaminopimelate

substrate: meso-diaminopimelate


enzyme_display_name: aspartate dehydrogenase

organism: Bradyrhizobium japonicum

assay_conditions_summary: pH 9.8; 301.15 K; cofactor NAD+

enzyme: aspartate dehydrogenase


assay_conditions_summary: pH 9.8; 301.15 K; cofactor NADP+


substrate_name: L-aspartate

substrate: L-aspartate


smiles: N


assay_conditions_summary: pH 9; 301.15 K; cofactor NADH


substrate_name: NADPH

assay_conditions_summary: pH 9; 301.15 K; cofactor NADPH

substrate: NADPH


substrate_name: oxaloacetate

substrate: oxaloacetate


enzyme_display_name: glutamate dehydrogenase [NAD(P)(+)]

organism: Tenebrio molitor

substrate_name: glutamate

smiles: C(CC(=O)O)C(C(=O)O)N

enzyme: glutamate dehydrogenase [NAD(P)(+)]

substrate: glutamate


enzyme_display_name: glutamate dehydrogenase (NADP(+))

organism: Saccharomyces cerevisiae

proof_lines:
oxidative deamination, Gdh3p gene
oxidative deamination, Gdh1p gene
oxidative deamination

enzyme: glutamate dehydrogenase (NADP(+))


assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADPH

proof_lines:
reductive amination, Gdh1p gene
reductive amination
reductive amination, Gdh3p gene


proof_lines:
reductive amination, Gdh3p gene
reductive amination, Gdh1p gene
isoform Gdh1, pH 7.5, 30°C


substrate_name: L-glutamate

substrate: L-glutamate


proof_lines:
isoform Gdh1, pH 5.8, 30°C
isoform Gdh1, pH 7.5, 30°C
reductive amination


enzyme_display_name: L-aspartate oxidase

organism: Gossypium hirsutum

enzyme: L-aspartate oxidase


organism: Thermococcus litoralis


enzyme_display_name: L-amino-acid oxidase

organism: Daboia russelii

substrate_name: L-Tyr

enzyme: L-amino-acid oxidase

substrate: L-Tyr


substrate_name: L-Phe

substrate: L-Phe


substrate_name: L-Trp

substrate: L-Trp


substrate_name: L-Met

smiles: CSCCC(C(=O)O)N

substrate: L-Met


substrate_name: L-Leu

smiles: CC(C)CC(C(=O)O)N

substrate: L-Leu


substrate_name: L-Ile

smiles: CCC(C)C(C(=O)O)N

substrate: L-Ile


substrate_name: L-Arg

smiles: C(CC(C(=O)O)N)CN=C(N)N

substrate: L-Arg


substrate_name: L-Val

smiles: CC(C)C(C(=O)O)N

substrate: L-Val


substrate_name: L-Lys

smiles: C(CCN)CC(C(=O)O)N

substrate: L-Lys


substrate_name: L-Ala

substrate: L-Ala


organism: Daboia russelii russelii

substrate_name: L-isoleucine

proof_lines:
isoform L1
isoform L2

substrate: L-isoleucine


substrate_name: L-phenylalanine

proof_lines:
isoform L2
isoform L1

substrate: L-phenylalanine


substrate_name: L-tyrosine

substrate: L-tyrosine


substrate_name: L-tryptophan

substrate: L-tryptophan


substrate_name: L-methionine

substrate: L-methionine


substrate_name: L-leucine

substrate: L-leucine


substrate_name: Unknown

proof_lines:
isoform L2, free enzyme
isoform L2, enzyme-substrate complex
isoform L1, enzyme-substrate complex

substrate: Unknown


enzyme_display_name: primary-amine oxidase

organism: Arthrobacter globiformis

substrate_name: benzylamine

assay_conditions_summary: pH 6.8; 288.15 K; buffer 50mM HEPES

enzyme: primary-amine oxidase

substrate: benzylamine


substrate_name: tyramine

substrate: tyramine


assay_conditions_summary: pH 6.8; 303.15 K; buffer 50mM HEPES

proof_lines:
pH 6.8, 30°C, Ni-activated enzyme
pH 6.8, 30°C, Co-activated enzyme
pH 6.8, 30°C, Ni2+-substituted enzyme


smiles: O=O

proof_lines:
pH 6.8, 30°C, Ni2+-substituted enzyme
pH 6.8, 30°C, Co2+-substituted enzyme
pH 6.8, 30°C, native copper protein


organism: Paenarthrobacter aurescens

substrate_name: spermidine

smiles: C(CCNCCCN)CN

assay_conditions_summary: pH 7; 310.15 K; buffer 100 mM potassium phosphate

proof_lines: isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C

substrate: spermidine


substrate_name: cadaverine

smiles: C(CCN)CCN

substrate: cadaverine


substrate_name: histamine

proof_lines: isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C

substrate: histamine


substrate_name: putrescine

smiles: C(CCN)CN

substrate: putrescine


enzyme_display_name: D-amino-acid oxidase

organism: Rasamsonia emersonii

substrate_name: D-arginine

enzyme: D-amino-acid oxidase

substrate: D-arginine


substrate_name: D-glutamate

substrate: D-glutamate


substrate_name: D-methionine

substrate: D-methionine


substrate_name: D-alanine

substrate: D-alanine


substrate_name: D-valine

substrate: D-valine


enzyme_display_name: monoamine oxidase

organism: Papio anubis

proof_lines: pH not specified in the publication, temperature not specified in the publication, MAO-B

enzyme: monoamine oxidase


organism: Rattus norvegicus

substrate_name: glycine

smiles: C(C(=O)O)N

substrate: glycine


enzyme_display_name: methylamine dehydrogenase (amicyanin)

organism: Paracoccus denitrificans

enzyme: methylamine dehydrogenase (amicyanin)


substrate_name: phenazine ethosulfate

substrate: phenazine ethosulfate


substrate_name: ethylamine

smiles: CCN

substrate: ethylamine


substrate_name: Propylamine

smiles: CCCN

substrate: Propylamine


substrate_name: methylamine

smiles: CN

proof_lines: pH 7.5, 30°C, mutant enzyme betaD32N, acceptor phenazine ethosulfate/2,6-dichlorophenolindophenol

proof_lines: pH 8.5, 30°C, mutant enzyme betaD32N, acceptor phenazine ethosulfate/2,6-dichlorophenolindophenol

proof_lines: pH 7.5, 30°C, mutant enzyme betaD32N, acceptor amicyanin

substrate: methylamine


proof_lines: pH 8.5, 30°C, wild-type enzyme, acceptor phenazine ethosulfate/2,6-dichlorophenolindophenol

proof_lines: pH 7.5, 30°C, wild-type enzyme, acceptor amicyanin

proof_lines: pH 8.5, 30°C, wild-type enzyme, acceptor amicyanin


substrate_name: Butylamine

smiles: CCCCN

substrate: Butylamine


proof_lines:
with phenylazine as electron acceptor
with amicyanin as electron acceptor


organism: Paracoccus versutus

assay_conditions_summary: pH 7

proof_lines: with amicyanin as electron acceptor at pH 7

proof_lines: with amicyanin as electron acceptor at pH 10


substrate_name: amicyanin

assay_conditions_summary: pH 7.5; buffer and 0

proof_lines: methylamine-reduced MADH, pH 7.5 using a Ca-HEPES puffer

proof_lines: dithionite-reduced MADH, pH 7.5 using a Na,K-phosphate buffer and 0.2 M KCl

proof_lines: methylamine-reduced MADH, pH 7.5 using a Na,K-phosphate buffer and 0.2 M KCl

substrate: amicyanin


enzyme_display_name: D-arginine dehydrogenase

organism: Helicobacter pylori

substrate_name: coenzyme Q1

assay_conditions_summary: pH 8; 310.15 K; buffer 50 mM sodium-phosphate

proof_lines: in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C

enzyme: D-arginine dehydrogenase

substrate: coenzyme Q1


substrate_name: D-Pro

proof_lines: with Q1 as the electron acceptor, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C

substrate: D-Pro


organism: Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504 = JCM 12093

substrate_name: D-proline

proof_lines:
Vmax: 12.3 micromol/min/mg
Vmax: 25 micromol/min/mg

substrate: D-proline


substrate_name: NADPH

mutation_signature: Δ1

assay_conditions_summary: cofactor NADPH

substrate: NADPH


substrate_name: NADH

proof_lines:
+ DELTA1-pyrroline-2-carboxylate
+ DELTA1-piperidine-2-carboxylate

substrate: NADH


proof_lines:
cosubstrate NADH
cosubstrate NADPH


organism: Homo sapiens

substrate_name: NAD+

assay_conditions_summary: cofactor NAD+

substrate: NAD+


assay_conditions_summary: pH 8; 303.15 K; cofactor NADP+

proof_lines: pH 8.0, 30°C, at saturating concentrations of NADP+ (6.0 mM)

proof_lines: pH 8.0, 30°C, at saturating concentrations of NAD+ (1.0 mM)


substrate_name: methenyltetrahydrofolate

substrate: methenyltetrahydrofolate


substrate_name: phosphate

substrate: phosphate


enzyme_display_name: pyrroline-5-carboxylate reductase

organism: Clostridium sporogenes

enzyme: pyrroline-5-carboxylate reductase


substrate_name: L-proline

substrate: L-proline


organism: Zymomonas mobilis subsp. mobilis