organism: Rattus norvegicus

substrate_name: cholesterol 7beta-hydroperoxide

assay_conditions_summary: pH 7.4; 298.15 K; buffer 50 mM potassium phosphate

proof_lines: in 50 mM potassium phosphate buffer (pH 7.4), at 25°C

substrate: cholesterol 7beta-hydroperoxide


enzyme_display_name: glutathione peroxidase

organism: Saccharomyces cerevisiae

source_db: brenda

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: pH 7.5, 25°C, reaction with cumene hydroperoxide

enzyme: glutathione peroxidase

source: brenda


organism: Thunnus maccoyii


enzyme_display_name: glutathione oxidase

organism: Homo sapiens

substrate_name: dithiothreitol

enzyme: glutathione oxidase

substrate: dithiothreitol


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


mutation_type: substitution


organism: Penicillium sp.

substrate_name: glutathione

proof_lines: immobilized enzyme on sensor surface out of cellulose acetate

substrate: glutathione


organism: Bacillus subtilis

substrate_name: 3'-phosphoadenylyl sulfate

assay_conditions_summary: pH 8

proof_lines: recombinant enzyme, pH 8.0, with thioredoxin 1

proof_lines: recombinant enzyme, pH 8.0, with substrate 3-adenylyl-sulfate

proof_lines: recombinant enzyme, pH 8.0, with glutaredoxin 1

substrate: 3'-phosphoadenylyl sulfate


organism: Escherichia coli

proof_lines: with cofactors Trx1, Trx2, or Grx1, pH 8.0


organism: Mycobacterium tuberculosis

substrate_name: adenosine 5'-phosphosulfate

verification_status: unverified

km_display: Not reported

assay_conditions_summary: pH 7.5; 303.15 K; buffer 100 mM bis-Tris propane

proof_lines: wild type enzyme, at 30°C in 100 mM bis-Tris propane buffer, pH 7.5

substrate: adenosine 5'-phosphosulfate

status: unverified


proof_lines: mutant enzyme K144A, at 30°C in 100 mM bis-Tris propane buffer, pH 7.5


organism: Pseudomonas aeruginosa

substrate_name: thioredoxin I

assay_conditions_summary: pH 7.5; 293.15 K; buffer 10 mM phosphate

proof_lines: wild type enzyme, at 20°C in 10 mM phosphate buffer (pH 7.5) containing 100 mM Na2SO4

substrate: thioredoxin I


substrate_name: thioredoxin

proof_lines: with thioredoxin from E. coli as cosubstrate

proof_lines: with thioredoxin from Saccharomyces cerevisiae as cosubstrate

substrate: thioredoxin


organism: Aspergillus oryzae

source_db: oed

protein_accession_database: UniProt

source: oed


substrate_name: D-Cys

substrate: D-Cys


organism: Mus musculus

substrate_name: histone octamer

source_db: uniprot

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

substrate: histone octamer

source: uniprot


proof_lines: All kinetic experiments are done with 10 mm Tris-HCl, 0.01% Triton X-100, and 10 mm DTT and at pH 8.5.


proof_lines: mutant enzyme S289N/S312W/S357Y, at pH 9.0 and 23°C


substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


enzyme_display_name: gellan tetrasaccharide unsaturated glucuronosyl hydrolase

organism: Bacillus sp. (in: firmicutes)


enzyme: gellan tetrasaccharide unsaturated glucuronosyl hydrolase


organism: Microbacterium sp. XT11

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

substrate: 4-nitrophenyl beta-D-glucopyranoside


proof_lines:
pH 6.5, 30°C, wild-type enzyme
pH 7.5, 30°C, wild-type enzyme


substrate_name: Unknown

ki_unit: mM

substrate: Unknown


organism: Bacillus sp. (strain GL1)

substrate_name: unsaturated chondroitin disaccharidee (delta0S)

mutation_signature: Δ6

sequence_variant_note: Resolved canonical sequence from source_record and applied mutation signature Δ6 to reconstruct the assayed variant sequence.

mutation_type: deletion

proof_lines: kcat is 14.1 sec(-1) with unsaturated chondroitin (delta0S). kcat is 54.9 sec(-1) with unsaturated chondroitin disaccharide sulfated at C-6 position of GalNAc residue (delta6S).


enzyme_display_name: [isocitrate dehydrogenase (NADP(+))] kinase

substrate_name: [Isocitrate dehydrogenase (NADP+)]; ATP

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

substrate: [Isocitrate dehydrogenase (NADP+)]; ATP


substrate_name: [isocitrate dehydrogenase (NADP+)]

assay_conditions_summary: pH 7; 310.15 K; cofactor NADP+

enzyme: [isocitrate dehydrogenase (NADP(+))] kinase

substrate: [isocitrate dehydrogenase (NADP+)]


substrate_name: [isocitrate dehydrogenase (NADP+)]N15L

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NADP+

proof_lines: pH 7.5, 37°C, kinase activity at saturating ATP

substrate: [isocitrate dehydrogenase (NADP+)]N15L


substrate_name: ATP

proof_lines: pH 7.5, 37°C, mutant AceK3, kinase activity

proof_lines: pH 7.5, 37°C, mutant AceK4, kinase activity

substrate: ATP


proof_lines: pH 7.5, 37°C, wild-type and mutant AceK4, kinase activity

proof_lines: pH 7.5, 37°C, wild-type, kinase activity


assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP


enzyme_display_name: methyl halide transferase

organism: Arabidopsis thaliana

substrate_name: S-Adenosyl-L-methionine; Thiocyanate

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

enzyme: methyl halide transferase

substrate: S-Adenosyl-L-methionine; Thiocyanate


organism: Batis maritima

substrate_name: chloride

proof_lines:
pH 6.8, 22°C, recombinant enzyme
pH 6.8, 22°C, native enzyme

substrate: chloride


organism: Raphanus sativus

proof_lines: pH 7.0, 30°C, purified recombinant enzyme


organism: Brassica oleracea

proof_lines:
pH 7.5, 22°C, cosubstrate: iodide
pH 7.5, 22°C


organism: Endocladia muricata


organism: Pavlova gyrans

substrate_name: Iodide

substrate: Iodide


organism: Pavlova pinguis


organism: Phellinus pomaceus


organism: Rhizobium sp.


enzyme_display_name: cytosine deaminase

organism: Saccharomyces cerevisiae

substrate_name: cytosine

source_db: skid

source_license: CC BY-NC-ND 4.0

enzyme: cytosine deaminase

substrate: cytosine

source: skid


enzyme_display_name: 2-methylcitrate dehydratase

organism: Escherichia coli

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

enzyme: 2-methylcitrate dehydratase


enzyme_display_name: poly(A)-specific ribonuclease

organism: Homo sapiens

substrate_name: Poly(A)

assay_conditions_summary: pH 7

proof_lines: residues 1-520 with the deletion of the R3H domain, pH 7.0, temperature not specified in the publication

proof_lines: residues 1-446 with the deletion of the R3H domain, pH 7.0, temperature not specified in the publication

enzyme: poly(A)-specific ribonuclease

substrate: Poly(A)


proof_lines: N-terminal fragment residues 1-520, pH 7.0, temperature not specified in the publication

proof_lines: N-terminal fragment residues 1-446, pH 7.0, temperature not specified in the publication

proof_lines: pH not specified in the publication, temperature not specified in the publication


proof_lines: subunit Bb, mutant with Cys-residues at positions 428, 435


enzyme_display_name: naringenin 7-O-methyltransferase

organism: Oryza sativa Japonica Group

proof_lines:
cultivar Nipponbare, pH 9.4, 30°C
cultivar Kasalath, pH 9.4, 30°C

enzyme: naringenin 7-O-methyltransferase


organism: Perilla frutescens

substrate_name: kaempferol

substrate: kaempferol


enzyme_display_name: cytokinin 7-beta-glucosyltransferase

substrate_name: UDPglucose; trans-Zeatin

enzyme: cytokinin 7-beta-glucosyltransferase

substrate: UDPglucose; trans-Zeatin


substrate_name: 3'-phosphoadenylyl sulfate

source_license: CC BY 4.0; CC BY-NC-ND 4.0

proof_lines: pH 9.0, 37°C, isozyme SOT18, ecotype C24

proof_lines: pH 9.0, 37°C, isozyme SOT18, ecotype Columbia-0

source_databases_merged: brenda skid

substrate: 3'-phosphoadenylyl sulfate


organism: Brassica napus


organism: Lachnoclostridium phytofermentans


enzyme_display_name: dual-specificity kinase

assay_conditions_summary: pH 6.8; 295.15 K; cofactor ATP

enzyme: dual-specificity kinase


substrate_name: desulfobenzylglucosinolate

proof_lines:
pH 9.0, 30°C
pH 9.0, 37°C, isozyme SOT17

substrate: desulfobenzylglucosinolate


enzyme_display_name: procollagen-proline 3-dioxygenase

enzyme: procollagen-proline 3-dioxygenase


organism: Oryza sativa

proof_lines: pH 8.5, 30°C, purified recombinant GST-tagged NOMT


enzyme_display_name: endoplasmic reticulum Man9GlcNAc2 1,2-alpha-mannosidase


enzyme: endoplasmic reticulum Man9GlcNAc2 1,2-alpha-mannosidase


proof_lines: 37°C, pH 7.1, pyridylamine-tagged Man9GlcNAc2, wild-type enzyme


organism: Candida albicans


proof_lines: 37°C, pH 6.6, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E330Q/E599Q


proof_lines: 37°C, pH 6.8, pyridylamine-tagged Man9GlcNAc2, mutant enzyme D463N


proof_lines: 37°C, pH 6.7, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E599Q


organism: Mus musculus

substrate_name: CMP-N-acetyl-beta-neuraminate

proof_lines: pH and temperature not specified in the publication

substrate: CMP-N-acetyl-beta-neuraminate


proof_lines: 37°C, pH 6.6, pyridylamine-tagged Man9GlcNAc2, mutant enzyme D463N/E599Q


proof_lines: 37°C, pH 6.9, pyridylamine-tagged Man9GlcNAc2, mutant enzyme H524A


proof_lines: 37°C, pH 7.1, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E330Q

proof_lines: 37°C, pH 5.3, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E330Q


proof_lines: truncated enzyme protein consisting of amino acids 26-340, hST3-DELTA25, pH and temperature not specified in the publication


proof_lines: truncated enzyme protein consisting of amino acids 57-340, hST3-DELTA56, pH and temperature not specified in the publication


substrate_name: ganglioside GM1a

substrate: ganglioside GM1a


enzyme_display_name: 4-hydroxybenzoate 1-hydroxylase

organism: Unknown

enzyme: 4-hydroxybenzoate 1-hydroxylase


substrate_name: complement component C3

substrate: complement component C3


substrate_name: cis-aconitate

substrate: cis-aconitate


organism: Gallus gallus

proof_lines: above, competitive inhibition with respect to 2-oxoglutarate and noncompetitive with respect to Fe2+ and the peptide substrate


organism: Rattus norvegicus

substrate_name: Procollagen

substrate: Procollagen


organism: Candida parapsilosis

proof_lines:
recombinant enzyme, pH 7.6, 25°C
pH 7.6, 30°C
3-chloro-4-hydroxybenzoate


organism: Eucalyptus nitida

substrate_name: pinocembrin

substrate: pinocembrin


substrate_name: S-adenosyl-L-methionine

proof_lines:
cultivar Nipponbare, pH 9.5, 30°C
cultivar Kassalath, pH 9.5, 30°C
cultivar Nipponbare, pH 9.5, 30°c

substrate: S-adenosyl-L-methionine


enzyme_display_name: gentamicin 3-N-acetyltransferase

substrate_name: Acetyl-CoA; Gentamicin C1a

assay_conditions_summary: cofactor CoA

enzyme: gentamicin 3-N-acetyltransferase

substrate: Acetyl-CoA; Gentamicin C1a


organism: Raphanus sativus

substrate_name: UDPglucose

substrate: UDPglucose


organism: Opitutus terrae

proof_lines: recombinant enzyme, pH 7.0, 37°C, reverse reaction

proof_lines: recombinant enzyme, pH 7.0, 37°C, forward reaction


substrate_name: gangloside asialo-GM1

proof_lines: pH 6.4, 37°C, reaction with ganglioside GM1

substrate: gangloside asialo-GM1


organism: Campylobacter jejuni


substrate_name: Ala-Leu-Gln-Asn-Ala-Thr-Arg

proof_lines:
solublilized enzyme
solubilized enzyme
membrane-bound enzyme

substrate: Ala-Leu-Gln-Asn-Ala-Thr-Arg


substrate_name: Tyr-Asn-Leu-Thr-Ser-Val

substrate: Tyr-Asn-Leu-Thr-Ser-Val


organism: Pseudomonas chlororaphis subsp. aureofaciens

substrate_name: cytosine

substrate: cytosine


organism: Saccharomyces cerevisiae

assay_conditions_summary: pH 7.5; 298.15 K; buffer PBS

proof_lines:
pH 7.5, 25°C, transient kinetics
pH 7.5, 25°C, steady-state kinetics
at pH 5,7 and 8


proof_lines: mutant E64D, pH 7.5, temperature not specified in the publication


proof_lines: mutant E64A, pH 7.5, temperature not specified in the publication


proof_lines: wild-type, pH 7.5, temperature not specified in the publication


organism: Salmonella enterica subsp. enterica serovar Typhimurium


organism: Serratia marcescens


organism: Yarrowia lipolytica


organism: Homo sapiens

substrate_name: poly(A) RNA

proof_lines:
mutant p74deltaR3H
mutant p54deltaR3H

substrate: poly(A) RNA


enzyme_display_name: (S)-methylmalonyl-CoA hydrolase

substrate_name: Malonyl-CoA; H2O

assay_conditions_summary: pH 6; 303.15 K; cofactor CoA

enzyme: (S)-methylmalonyl-CoA hydrolase

substrate: Malonyl-CoA; H2O


proof_lines: hydrolysis of the fluorogenic substrate follows hyperbolic kinetics

proof_lines: MUaMan, fluorogenic substrate. Vmax 36.2 nmol of 4-methylumbelliferone min-1 mg-1 of protein


organism: Sporothrix schenckii

assay_conditions_summary: pH 7; 310.15 K; buffer 50 mM MES-Tris

proof_lines: at pH 7.0 in 50 mM MES-Tris buffer and at 37°C


substrate_name: complement component C5

proof_lines: high-affinity C5 convertase (EMan,C3bC4b,C2a)

substrate: complement component C5


organism: Aspergillus fumigatus


organism: Aspergillus niger


organism: Chromobacterium violaceum


organism: Escherichia coli

proof_lines:
pH 7.5, 25°C, Zn2+CDase
pH 7.5, 25°C, CDase
pH 7.3, 37°C


organism: Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)

substrate_name: L-rhamnose

substrate: L-rhamnose


substrate_name: CMP-N-acetyl-beta-neuraminate

substrate: CMP-N-acetyl-beta-neuraminate


substrate_name: ganglioside GA1

substrate: ganglioside GA1


organism: Arabidopsis thaliana

substrate_name: desulfobenzyl glucosinolate (in cv. Columbia)


substrate_name: desulfo-benzyl glucosinolate

substrate: desulfo-benzyl glucosinolate


organism: Escherichia coli (strain K12)


organism: Yarrowia lipolytica (strain CLIB 122 / E 150)


proof_lines:
mutant D314A, pH 7.5, 37°C
pH 7.5, 37°C, mutant D314A


proof_lines: mutant enzyme D314E/F316L/D317G, pH and temperature not specified in the publication


proof_lines: mutant enzyme V152A/F316C/D317G, pH and temperature not specified in the publication


proof_lines: mutant enzyme V315L/F316V/D317G, pH and temperature not specified in the publication


proof_lines:
wild-type, pH 8.5, 30°C
pH 7.5, 25°C, wild-type enzyme
wild-type, pH 7.5, 37°C


proof_lines: reaction with dolichyl diphosphochitobiose-Man9Glc3


organism: Sus scrofa


organism: Arachis hypogaea

substrate_name: ATP

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

proof_lines: versus ATP, pH 7.5, 30°C, recombinant mutant Y148F

proof_lines: versus histone H1, pH 7.5, 30°C, recombinant mutant Y148F

substrate: ATP


substrate_name: histone H1

proof_lines: versus ATP, pH 7.5, 30°C, recombinant mutant Y297F

substrate: histone H1


proof_lines: versus ATP, pH 7.5, 30°C, recombinant mutant Y317F

proof_lines: versus histone H1, pH 7.5, 30°C, recombinant mutant Y317F


proof_lines: versus ATP, pH 7.5, 30°C, recombinant mutant Y213F


proof_lines: versus ATP, pH 7.5, 30°C, recombinant wild-type enzyme

proof_lines: versus histone H1, pH 7.5, 30°C, recombinant wild-type enzyme


substrate_name: glucoerucin

assay_conditions_summary: pH 9; 310.15 K; buffer conditions and substrate names

proof_lines: in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C

substrate: glucoerucin


organism: Bos taurus

substrate_name: Poly(A)

substrate: Poly(A)


mutation_signature: C-terminal truncated form C-terminal truncated form

mutation_type: truncation


proof_lines:
full-length 74 kDa PARN
54 kDa form
46 kDa form


enzyme_display_name: phosphomevalonate kinase

organism: Enterococcus faecalis

substrate_name: ATP; (R)-5-Phosphomevalonate

assay_conditions_summary: cofactor ATP

enzyme: phosphomevalonate kinase

substrate: ATP; (R)-5-Phosphomevalonate


substrate_name: (R)-mevalonate 5-phosphate

assay_conditions_summary: pH 7; 303.15 K; cofactor ATP

proof_lines: pH 7.0, 30°C, recombinant wild-type enzyme

substrate: (R)-mevalonate 5-phosphate


substrate_name: ADP

substrate: ADP


substrate_name: (R)-mevalonate 5-diphosphate

substrate: (R)-mevalonate 5-diphosphate


enzyme_display_name: phosphonoacetaldehyde hydrolase

organism: Bacillus cereus

substrate_name: phosphonoacetaldehyde

enzyme: phosphonoacetaldehyde hydrolase

substrate: phosphonoacetaldehyde


proof_lines: triple mutant K121R/K146R/K192R, pH 7.5, 25°C


proof_lines: wild-type enzyme and mutant M49L, pH 7.5, 25°C

proof_lines: wild-type enzyme, mutant C22S, and mutant Y128F/C22S, pH 7.5, 25°C


organism: Saccharomyces cerevisiae

assay_conditions_summary: pH 7.2; 310.15 K; cofactor ATP

proof_lines:
at pH 7.2 and 37°C
at pH 7.2 and 30°C


organism: Sulfolobus solfataricus

substrate_name: (R)-5-Phosphomevalonate; ATP

substrate: (R)-5-Phosphomevalonate; ATP


smiles: O=CCP(=O)(O)O


enzyme_display_name: nigerose phosphorylase

organism: Lachnoclostridium phytofermentans

substrate_name: methyl alpha-D-glucopyranoside

enzyme: nigerose phosphorylase

substrate: methyl alpha-D-glucopyranoside


substrate_name: D-glucose

substrate: D-glucose


substrate_name: D-galactose

proof_lines: pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate

proof_lines: pH 7.0, 30°C, reverse phosphorolysis, acceptor D-glucose

substrate: D-galactose


substrate_name: H2O; Phosphonoacetaldehyde

substrate: H2O; Phosphonoacetaldehyde


proof_lines:
pH 7.5, 25°C
Raw SKiD Env: Temp=25°C, pH=7.5


substrate_name: D-Xylose

substrate: D-Xylose


enzyme_display_name: ketosteroid monooxygenase

organism: Rhodococcus rhodochrous

substrate_name: phenylacetone

proof_lines: mutant enzyme T345L, in 50 mM Tris/HCl, pH 7.5, temperature not specified in the publication

enzyme: ketosteroid monooxygenase

substrate: phenylacetone


proof_lines: kcat_Km less than 0.1 1/sec*min, mutant enzyme L500Y, in 50 mM Tris/HCl, pH 7.5, temperature not specified in the publication


proof_lines: wild type enzyme, in 50 mM Tris/HCl, pH 7.5, temperature not specified in the publication


proof_lines: mutant enzyme K295A, in 50 mM Tris/HCl, pH 7.5, temperature not specified in the publication


proof_lines: pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate

proof_lines: pH 7.0, 30°C, cosubstrate 3-O-alpha-D-glucopyranosyl-D-glucopyranose


substrate_name: beta-D-glucopyranose 1-phosphate

substrate: beta-D-glucopyranose 1-phosphate


proof_lines: mutant enzyme V291A, in 50 mM Tris/HCl, pH 7.5, temperature not specified in the publication


proof_lines: mutant enzyme P157Q, in 50 mM Tris/HCl, pH 7.5, temperature not specified in the publication


substrate_name: NAD+; Coenzyme A; alpha-Ketoisovalerate

assay_conditions_summary: cofactor NAD+

substrate: NAD+; Coenzyme A; alpha-Ketoisovalerate


substrate_name: Dihydrolipoamide; 3-Methylbutanoyl-CoA

substrate: Dihydrolipoamide; 3-Methylbutanoyl-CoA


organism: Paenarthrobacter nicotinovorans

proof_lines:
wild-type, PBS, pH 6.4, 30°C
wild-type, Tris/HCl, pH 8.5, 30°C
wild-type, Tris/HCl, pH 8.0, 30°C


enzyme_display_name: leishmanolysin

organism: Leishmania donovani donovani

substrate_name: casein

enzyme: leishmanolysin

substrate: casein


proof_lines: fluorogenic substrate, pH 7.2, 37°C, truncated mutant DELTA484


proof_lines: fluorogenic substrate, pH 7.2, 37°C, mutant F448Y


proof_lines: fluorogenic substrate, pH 7.2, 37°C, wild-type enzyme


organism: Leishmania mexicana

substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication

substrate: Unknown


enzyme_display_name: 2-hydroxyacyl-CoA lyase

organism: Actinomycetospora chiangmaiensis

assay_conditions_summary: pH 7.2; 310.15 K; cofactor CoA

enzyme: 2-hydroxyacyl-CoA lyase


organism: Rattus norvegicus

substrate_name: NAD+; Coenzyme A; 2-Oxoisocaproate

substrate: NAD+; Coenzyme A; 2-Oxoisocaproate


enzyme_display_name: staphopain

organism: Staphylococcus aureus

substrate_name: cystatin C

mutation_signature: N-terminal truncation shown to impair inhibition of additional targets / N-terminal truncation shown to impair inhibition of additional targets

proof_lines: Gly11 bond hydrolyzed by staphopain A, N-terminal truncation shown to impair inhibition of additional targets, disturbance of the host protease-inhibitor balance

enzyme: staphopain

substrate: cystatin C


substrate_name: cystatin D

mutation_signature: truncation of cystatin D shown to cause alleviated inhibition of endogenous target enzymes investigated / truncation of cystatin D shown to cause alleviated inh

proof_lines: Ala10 bond hydrolyzed by staphopain A, truncation of cystatin D shown to cause alleviated inhibition of endogenous target enzymes investigated, disturbance of the host protease-inhibitor balance

substrate: cystatin D


organism: Leishmania amazonensis

assay_conditions_summary: pH 10

proof_lines:
pH 10.0, 37Â°C
pH 10.0, 37Â°C, with secreted gp63


proof_lines: fluorogenic substrate, pH 7.2, 37°C, truncated mutant DELTA389


proof_lines: fluorogenic substrate, pH 7.2, 37°C, mutant F448V


proof_lines: fluorogenic substrate, pH 7.2, 37°C, mutant S446T


proof_lines: fluorogenic substrate, pH 7.2, 37°C, truncated mutant DELTA542


proof_lines: fluorogenic substrate, pH 7.2, 37°C, truncated mutant DELTA420


proof_lines: fluorogenic substrate, pH 7.2, 37°C, mutant S446A


organism: Homo sapiens

substrate_name: NAD+; Coenzyme A; alpha-Ketovaline

substrate: NAD+; Coenzyme A; alpha-Ketovaline


organism: Rhodospirillales bacterium

substrate_name: formaldehyde

assay_conditions_summary: pH 7.4; 310.15 K; cofactor CoA

substrate: formaldehyde


proof_lines:
pH 7.4, 37°C
at pH 7.5 and 37°C


enzyme_display_name: methyltransferase cap1

organism: Dengue virus type 2 (strain Puerto Rico/PR159-S1/1969)

substrate_name: N-acetyl-Gly-Arg-Arg-AMC

enzyme: methyltransferase cap1

substrate: N-acetyl-Gly-Arg-Arg-AMC


organism: Kokobera virus

substrate_name: ATPase of serine protease NS3

substrate: ATPase of serine protease NS3


organism: Tick-borne encephalitis virus European subtype (strain Neudoerfl)

substrate_name: ATP

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

substrate: ATP


organism: Trypanosoma brucei brucei (strain 927/4 GUTat10.1)

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


enzyme_display_name: 1,3-beta-glucan synthase

organism: Cryptococcus neoformans (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487)

substrate_name: UDP-glucose (at pH 7.75)

enzyme: 1,3-beta-glucan synthase

substrate: UDP-glucose (at pH 7.75)


organism: Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

substrate_name: (R,S)-5-phosphomevalonate (at 37 degrees Celsius)


organism: Sus scrofa

substrate_name: phosphomevalonate

substrate: phosphomevalonate


organism: Bacillus cereus

substrate_name: phosphonoacetaldehyde (at pH 7.0, 25 degrees Celsius)


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)


organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

substrate: phosphonoacetaldehyde (at pH 7.0, 25 degrees Celsius)


substrate_name: CBZ-Phe-Leu-Glu-pNA

substrate: CBZ-Phe-Leu-Glu-pNA


enzyme_display_name: naringenin 7-O-methyltransferase

organism: Ocimum basilicum

substrate_name: apigenin (in the presence of S-adenosyl-L-methionine)

proof_lines: kcat is 43x10(-3) sec(-1) with apigenin as substrate (in the presence of S-adenosyl-L-methionine) (PubMed:22923679). kcat is 49x10(-3) sec(-1) with luteolin as substrate (in the presence of S-adenosyl-L-methionine) (PubMed:22923679). kcat i

enzyme: naringenin 7-O-methyltransferase


proof_lines: kcat is 91x10(-3) sec(-1) with apigenin as substrate (in the presence of S-adenosyl-L-methionine) (PubMed:22923679). kcat is 100x10(-3) sec(-1) with luteolin as substrate (in the presence of S-adenosyl-L-methionine) (PubMed:22923679). kcat

substrate: apigenin (in the presence of S-adenosyl-L-methionine)


organism: Oryza sativa subsp. japonica

substrate_name: naringenin

proof_lines: kcat is 25 sec(-1) with naringenin as substrate.

substrate: naringenin


organism: Dengue virus type 2 (strain Thailand/NGS-C/1944)

substrate_name: triphosphorylated RNA

proof_lines: Vmax with 0.65 nmol/s/ug enzyme for RTPase activity with triphosphorylated RNA as substrate.

substrate: triphosphorylated RNA


organism: Severe acute respiratory syndrome coronavirus

substrate_name: peptide TSAVLQSGFRK-NH(2)

proof_lines: The kinetic parameters are studied for the 3C-like proteinase domain.

substrate: peptide TSAVLQSGFRK-NH(2)


organism: Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)

substrate_name: nigerose

proof_lines: kcat is 67 sec(-1) for nigerose. kcat is 110 sec(-1) for D-glucose.

substrate: nigerose


organism: Cordyceps militaris (strain CM01)

proof_lines: kcat is 1.42 min(-1) for UDP-glucose (at 37 degrees Celsius and pH 7).


organism: Mus musculus

proof_lines: Vmax is 10 fold higher with Gal-beta-1,3-GalNAc than with Gal-beta-1,3-GlcNAc as substrate.


proof_lines: Vmax is 4 fold higher with Gal-beta-1,3-GalNAc than with Gal-beta-1,3-GlcNAc as substrate.


proof_lines: Relative Vmax is 2:5:1 for Gal-beta-1,3-GlcNAc, Gal-beta-1,4-GlcNAc and Gal-beta-1,3-GalNAc as substrate, respectively.


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: an Asn-Tyr-Thr tripeptide

proof_lines: Glycosylates at a rate of 3.5 peptides per minute per OST.

substrate: an Asn-Tyr-Thr tripeptide


organism: Caenorhabditis elegans

substrate_name: mei-1 (Isoform a)

proof_lines: The presence of egg-4 in the assay increases the KM of mbk-2 for mei-1.

substrate: mei-1 (Isoform a)


organism: Rattus norvegicus

data_origin: experimental


organism: Arabidopsis thaliana

verification_status: corrected

proof_lines: kcat is 20.9 sec(-1) with (R)-5-phosphomevalonate.

status: corrected


organism: Homo sapiens

substrate_name: complement C3

proof_lines: kcat is 1.78 sec(-1) with complement C3 as substrate.

substrate: complement C3


enzyme_display_name: cytosine deaminase

organism: Escherichia coli (strain K12)

substrate_name: cytosine

proof_lines: kcat is 185 sec(-1) for the deamination of cytosine using Fe(2+) as cofactor, kcat is 92 sec(-1) using Mn(2+) as cofactor, kcat is 52 sec(-1) using Co(2+) as cofactor, and kcat is 32 sec(-1) using Zn(2+) as cofactor (PubMed:8226944). kcat i

enzyme: cytosine deaminase

substrate: cytosine


proof_lines: kcat is 91 sec(-1) with cytosine as substrate and 17 sec(-1) with 5-fluorocytosine as substrate.


organism: Aspergillus fumigatus

substrate_name: UDPglucose

substrate: UDPglucose


organism: Cryptococcus neoformans

substrate_name: UDP-glucose

substrate: UDP-glucose


organism: Euglena gracilis


organism: Exophiala dermatitidis


organism: Gossypium hirsutum


organism: Nakaseomyces glabratus

proof_lines:
enzyme from wild-type strain 3168
enzyme from wild-type strain 218
enzyme from wild-type strain 90030


organism: Neurospora crassa


organism: Papiliotrema laurentii


organism: Saccharomyces cerevisiae


organism: Saprolegnia monoica

proof_lines:
1,3-beta-glucan synthase 2
1,3-beta-glucan synthase 1


organism: Schizophyllum commune


organism: Staphylococcus aureus


organism: Mus musculus


organism: Rattus norvegicus

substrate_name: N-palmitoylphosphatidylethanolamine

proof_lines: 37°C, pH not specified in the publication, Triton X-100 stimulated enzyme

substrate: N-palmitoylphosphatidylethanolamine


organism: Ilyonectria destructans

substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

proof_lines: with androstenedione and O2 as cosubstrates

proof_lines: with progesterone and NADPH as cosubstrates

proof_lines: with progesterone and O2 as cosubstrates

substrate: NADPH


organism: Neonectria radicicola

substrate_name: H+; NADPH; O2; Progesterone

assay_conditions_summary: cofactor NADP+

substrate: H+; NADPH; O2; Progesterone


organism: Rhodococcus rhodochrous

assay_conditions_summary: pH 8.3; 310.15 K; cofactor NADPH


organism: Dengue virus

substrate_name: S-adenosyl-L-methionine

assay_conditions_summary: pH 10; 295.15 K; buffer glycine

proof_lines: apparent value, in glycine buffer (pH 10.0), at 22°C

substrate: S-adenosyl-L-methionine


organism: Usutu virus

proof_lines: methyltransferase domain of NS5, at pH 7.4 and 30°C


organism: Bos taurus

substrate_name: isovaleryl-CoA

substrate: isovaleryl-CoA


organism: Oryctolagus cuniculus

substrate_name: NAD+; Coenzyme A; 3-Methyl-2-oxopentanoate


organism: Achlya ambisexualis


organism: Arabidopsis thaliana


organism: Arachis hypogaea


enzyme_display_name: corrinoid adenosyltransferase EutT

organism: Listeria monocytogenes serotype 1/2a

substrate_name: cob(II)alamin

proof_lines: mutant D110N, pH not specified in the publication, temperature not specified in the publication

proof_lines: mutant D110N, Khalf value, Hill coefficient 1.2, pH not specified in the publication, temperature not specified in the publication

proof_lines: mutant D110N, Khalf value, Hill coefficient 2.5, pH not specified in the publication, temperature not specified in the publication

enzyme: corrinoid adenosyltransferase EutT

substrate: cob(II)alamin


substrate_name: ATP

proof_lines: mutant E207Q, pH not specified in the publication, temperature not specified in the publication

proof_lines: mutant E207Q, Khalf value, Hill coefficient 2.4, pH not specified in the publication, temperature not specified in the publication

proof_lines: mutant E207Q, Khalf value, Hill coefficient 1.7, pH not specified in the publication, temperature not specified in the publication

substrate: ATP


proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication

proof_lines: wild-type, Khalf value, Hill coefficient 1.9, pH not specified in the publication, temperature not specified in the publication

proof_lines: wild-type, Khalf value, Hill coefficient 2.3, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant T88V, pH not specified in the publication, temperature not specified in the publication

proof_lines: mutant T88V, Khalf value, Hill coefficient 1.8, pH not specified in the publication, temperature not specified in the publication

proof_lines: mutant T88V, Khalf value, Hill coefficient 1.2, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant K98Q, pH not specified in the publication, temperature not specified in the publication

proof_lines: mutant K98Q, Khalf value, Hill coefficient 1.7, pH not specified in the publication, temperature not specified in the publication

proof_lines: mutant K98Q, Khalf value, Hill coefficient 0.9, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant R200K, pH not specified in the publication, temperature not specified in the publication

proof_lines: mutant R200K, Khalf value, Hill coefficient 1.3, pH not specified in the publication, temperature not specified in the publication

proof_lines: mutant R200K, Khalf value, Hill coefficient 1.9, pH not specified in the publication, temperature not specified in the publication


substrate_name: ATP; (R)-5-Phosphomevalonate

substrate: ATP; (R)-5-Phosphomevalonate


organism: Catharanthus roseus


organism: Enterococcus faecalis


organism: Hevea brasiliensis

assay_conditions_summary: pH 7.2; 303.15 K; cofactor ATP


organism: Homo sapiens


proof_lines:
wild-type, 30°C, pH 7.0
wild-type, 30°C, pH 8.0


enzyme_display_name: arsenate reductase (donor)

organism: Oryza sativa

substrate_name: reduced glutaredoxin

enzyme: arsenate reductase (donor)

substrate: reduced glutaredoxin


substrate_name: arsenate

substrate: arsenate


enzyme_display_name: urethanase

organism: Candida parapsilosis

substrate_name: ethyl carbamate

proof_lines: mutant N194V, 40°C, pH not specified in the publication

enzyme: urethanase

substrate: ethyl carbamate


substrate_name: 2-aminomuconate semialdehyde

proof_lines: Cu-substituted enzyme, pH 7.0, temperature not specified in the publication

proof_lines: enzyme with 64.4% zinc ion occupancy, pH 7.0, temperature not specified in the publication

substrate: 2-aminomuconate semialdehyde


organism: Rattus norvegicus

proof_lines:
pH 6.0, 25°C, kidney enzyme
pH 6.0, 25°C, liver enzyme


enzyme_display_name: streptomycin 3''-adenylyltransferase

organism: Pseudomonas aeruginosa

assay_conditions_summary: pH 7.5; 296.15 K; cofactor ATP

enzyme: streptomycin 3''-adenylyltransferase


mutation_signature: C-terminal deletion mutant

proof_lines: C-terminal deletion mutant, pH 7.5, 23°C


substrate_name: spectinomycin

substrate: spectinomycin


substrate_name: streptomycin

substrate: streptomycin


enzyme_display_name: 2-phosphinomethylmalate synthase

organism: Streptomyces hygroscopicus

substrate_name: acetyl-CoA

assay_conditions_summary: pH 7.8; 298.15 K; cofactor CoA

proof_lines: pH 7.8, 25°C, mutant enzyme W112A, apparent KM value from Michaelis-Menten plots due to inability to accurately measure activity at substrate concentrations less than 0.0025 mM

enzyme: 2-phosphinomethylmalate synthase

substrate: acetyl-CoA


proof_lines: pH 7.8, 25°C, mutant enzyme E82A, apparent KM value from Michaelis-Menten plots due to inability to accurately measure activity at substrate concentrations less than 0.0025 mM


organism: Bifidobacterium bifidum

substrate_name: H2O; 2'-Fucosyllactose

substrate: H2O; 2'-Fucosyllactose


organism: Streptosporangium roseum

proof_lines: at 37°C, pH not specified in the publication


organism: Thermotoga maritima

substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication

substrate: Unknown


enzyme_display_name: glutamin-(asparagin-)ase

organism: Acinetobacter glutaminasificans

substrate_name: H2O; L-Glutamine

enzyme: glutamin-(asparagin-)ase

substrate: H2O; L-Glutamine


substrate_name: oxaloacetic acid

assay_conditions_summary: pH 8; 303.15 K; cofactor CoA

substrate: oxaloacetic acid


proof_lines: pH 7.8, 25°C, mutant enzyme E82Q, apparent KM value from Michaelis-Menten plots due to inability to accurately measure activity at substrate concentrations less than 0.0025 mM


proof_lines: pH 7.8, 25°C, mutant enzyme R173A, apparent KM value from Michaelis-Menten plots due to inability to accurately measure activity at substrate concentrations less than 0.0025 mM


enzyme_display_name: acetylputrescine deacetylase

substrate_name: Boc-Lys-(Ac)-AMC

enzyme: acetylputrescine deacetylase

substrate: Boc-Lys-(Ac)-AMC


proof_lines: mutant T111L, 40°C, pH not specified in the publication


proof_lines: wild-type, 40°C, pH not specified in the publication


proof_lines: mutant Y133A, 40°C, pH not specified in the publication


enzyme_display_name: reticuline oxidase

organism: Eschscholzia californica

substrate_name: (S)-scoulerine

enzyme: reticuline oxidase

substrate: (S)-scoulerine


organism: Shewanella sediminis

substrate_name: tetrachloroethene

assay_conditions_summary: pH 8

proof_lines: pH 8.0, temperature not specified in the publication

substrate: tetrachloroethene


organism: Vitis vinifera

substrate_name: coumaroyl-CoA

assay_conditions_summary: pH 6.5; 303.15 K; cofactor CoA

substrate: coumaroyl-CoA


substrate_name: cyanidin 3-O-beta-D-glucoside

substrate: cyanidin 3-O-beta-D-glucoside


substrate_name: delphinidin 3-O-beta-D-glucoside

substrate: delphinidin 3-O-beta-D-glucoside


substrate_name: cyanidin 3-O,5-O-beta-D-diglucoside

substrate: cyanidin 3-O,5-O-beta-D-diglucoside


substrate_name: arsenate

substrate: arsenate


substrate_name: (S)-reticuline

substrate: (S)-reticuline


assay_conditions_summary: cofactor FAD


substrate_name: caffeoyl-CoA

substrate: caffeoyl-CoA


substrate_name: malonyl-CoA

substrate: malonyl-CoA


substrate_name: malvidin 3-O-beta-D-glucoside

substrate: malvidin 3-O-beta-D-glucoside


enzyme_display_name: flavonoid 4'-O-methyltransferase

organism: Streptomyces sp. KCTC 0041BP

substrate_name: S-adenosyl-L-methionine

enzyme: flavonoid 4'-O-methyltransferase

substrate: S-adenosyl-L-methionine


substrate_name: quercetin

substrate: quercetin


substrate_name: peonidin 3-O-beta-D-glucoside

substrate: peonidin 3-O-beta-D-glucoside


enzyme_display_name: meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase I

organism: Mycobacterium tuberculosis

substrate_name: Unknown

substrate: Unknown


enzyme_display_name: 1-deoxy-D-xylulose-5-phosphate synthase

organism: Escherichia coli

substrate_name: D-Glyceraldehyde 3-phosphate; Pyruvate

enzyme: 1-deoxy-D-xylulose-5-phosphate synthase

substrate: D-Glyceraldehyde 3-phosphate; Pyruvate


substrate_name: D-glyceraldehyde 3-phosphate

proof_lines: pH 8, temperature not specified in the publication

substrate: D-glyceraldehyde 3-phosphate


substrate_name: pyruvate

proof_lines: wild-type, pH 8.0, temperature not specified in the publication

substrate: pyruvate


proof_lines: mutant lacking loop 190-234, pH 8.0, temperature not specified in the publication


substrate_name: guanine9 in tRNAValUAC

substrate: guanine9 in tRNAValUAC


organism: Saccharomyces cerevisiae

substrate_name: guanine9 in tRNAGlyGCC

proof_lines: tRNA from Schizosaccharomyces pombe, recombinant enzyme, pH and temperature not specified in the publication

substrate: guanine9 in tRNAGlyGCC


organism: Schizosaccharomyces pombe

substrate_name: guanine9 in tRNAGly

substrate: guanine9 in tRNAGly


organism: Thermococcus kodakarensis

proof_lines: pH 8.0, 40°C, recombinant enzyme, tRNA substrate from Saccharomyces cerevisiae

proof_lines: pH 8.0, 50°C, recombinant enzyme, tRNA substrate from Thermococcus kodakarensis


organism: EDTA-degrading bacterium BNC1

substrate_name: ethylenediaminetetraacetate

proof_lines: mutant P291A, presence of Mg2+, pH 7.6, temperature not specified in the publication

substrate: ethylenediaminetetraacetate


proof_lines: mutant Q58A, presence of Mg2+, pH 7.6, temperature not specified in the publication


proof_lines: mutant R68A, presence of Mg2+, pH 7.6, temperature not specified in the publication


proof_lines: mutant W11A, presence of Mg2+, pH 7.6, temperature not specified in the publication


enzyme_display_name: dibenzothiophene monooxygenase

organism: Paenibacillus sp.

substrate_name: benzothiophene

enzyme: dibenzothiophene monooxygenase

substrate: benzothiophene


organism: Rhodococcus erythropolis

substrate_name: dibenzothiophene

proof_lines: mutant enzyme A101K/W327C, calculated, pH and temperature not specified in the publication

substrate: dibenzothiophene


proof_lines: mutant enzyme A101K, calculated, pH and temperature not specified in the publication


organism: Plasmodium vivax

proof_lines: pH and temperature not specified in the publication


organism: Pseudomonas aeruginosa


enzyme_display_name: mannosyl-3-phosphoglycerate synthase

organism: Pyrococcus horikoshii

substrate_name: Glycerate 3-phosphate; GDPmannose

enzyme: mannosyl-3-phosphoglycerate synthase

substrate: Glycerate 3-phosphate; GDPmannose


organism: Rubrobacter xylanophilus

substrate_name: GDP-mannose

proof_lines: A164S/A208S/F211Y mutant, with GDP-mannose

substrate: GDP-mannose


proof_lines: mutant enzyme W327C, calculated, pH and temperature not specified in the publication


proof_lines: wild-type enzyme, calculated, pH and temperature not specified in the publication


proof_lines: mutant enzyme A101K/W327C/P413A, calculated, pH and temperature not specified in the publication


proof_lines: mutant Q294A, presence of Mg2+, pH 7.6, temperature not specified in the publication


proof_lines: wild-type, presence of Mg2+ and flavin reductase from Escherichia coli, pH 7.6, temperature not specified in the publication

proof_lines: wild-type, presence of Mg2+ and oxidoreductase EmoB, pH 7.6, temperature not specified in the publication

proof_lines: wild-type, presence of Mg2+, pH 7.6, temperature not specified in the publication


smiles: O=C(O)CN(CCN(CC(=O)O)CC(=O)O)CC(=O)O


proof_lines: no addition of divalent ions, pH 7.8, 35°C


enzyme_display_name: aliphatic sulfonate oxidoreductase

organism: Pyrococcus furiosus

substrate_name: hexanal

proof_lines:
at pH 8.0 and 80Â°C
at pH 8.4 and 60Â°C

enzyme: aliphatic sulfonate oxidoreductase

substrate: hexanal


enzyme_display_name: fatty acid kinase

organism: Cereibacter sphaeroides

substrate_name: valerate

sequence_source: UniProtKB Q3IYF1 direct accession check; sequence exists but is not emitted in this exact-accession lane

smiles: CCCCC(=O)O

enzyme: fatty acid kinase

substrate: valerate


substrate_name: acetate

smiles: CC(=O)O

substrate: acetate


substrate_name: ATP

assay_conditions_summary: pH 5.7; 310.15 K; cofactor ATP

proof_lines: with acetate as cosubstrate, at pH 5.7 and 37°C

substrate: ATP


proof_lines:
wild-type
wild-type, with GDP-mannose


organism: Thermus thermophilus


enzyme_display_name: oleanolate 3-O-glucosyltransferase

organism: Barbarea vulgaris

substrate_name: UDP-alpha-D-glucose

enzyme: oleanolate 3-O-glucosyltransferase

substrate: UDP-alpha-D-glucose


substrate_name: Isobutyrate

smiles: CC(C)C(=O)O

substrate: Isobutyrate


substrate_name: propionate

substrate: propionate


substrate_name: Butyrate

smiles: CCCC(=O)O

substrate: Butyrate


enzyme_display_name: amine sulfotransferase

organism: Homo sapiens

enzyme: amine sulfotransferase


organism: Arabidopsis thaliana


substrate_name: Inositol 1,3,4,5-tetrakisphosphate

proof_lines: mutant enzyme F593D/L597D, at pH 7.0 and 23°C

substrate: Inositol 1,3,4,5-tetrakisphosphate


organism: Mus musculus


organism: Oryctolagus cuniculus


organism: Oryza sativa

substrate_name: H2O; 4-Nitrophenyl phosphate

substrate: H2O; 4-Nitrophenyl phosphate


organism: Rattus norvegicus


enzyme_display_name: (R)-specific secondary-alkylsulfatase (type III)

organism: Pseudomonas sp. DSM 6611

substrate_name: (2R)-octan-2-yl hydrogen sulfate

proof_lines: mutant H317A, pH not specified in the publication, temperature not specified in the publication

substrate: (2R)-octan-2-yl hydrogen sulfate


proof_lines: mutant S233Y, pH not specified in the publication, temperature not specified in the publication

enzyme: (R)-specific secondary-alkylsulfatase (type III)


proof_lines: mutant F250G, pH not specified in the publication, temperature not specified in the publication


proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant S233F/F250S, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant S233F/F250G, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant S233Y/F250G, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant Y417F, pH not specified in the publication, temperature not specified in the publication


enzyme_display_name: sialate O-acetylesterase

organism: Danio rerio

substrate_name: 4-nitrophenyl acetate

enzyme: sialate O-acetylesterase

substrate: 4-nitrophenyl acetate


organism: Tannerella forsythia


proof_lines: mutant Y417H, pH not specified in the publication, temperature not specified in the publication


enzyme_display_name: isoamylase

substrate_name: amylopectin

enzyme: isoamylase

substrate: amylopectin


enzyme_display_name: inosine nucleosidase

organism: Bacillus thuringiensis

substrate_name: Inosine

enzyme: inosine nucleosidase

substrate: Inosine


enzyme_display_name: gingipain R

organism: Porphyromonas gingivalis

substrate_name: Unknown

proof_lines: pH 7.6, HRgpA and RgpB, in absence of Zn2+

enzyme: gingipain R

substrate: Unknown


enzyme_display_name: asclepain

organism: Asclepias curassavica

enzyme: asclepain


substrate_name: Nalpha-CBZ-L-Gln-4-nitrophenyl ester

substrate: Nalpha-CBZ-L-Gln-4-nitrophenyl ester


enzyme_display_name: phytepsin

organism: Cynara cardunculus

proof_lines: native mature enzyme, at pH 4.0 and 37°C

proof_lines: recombinant proenzyme with plant-specific insert domain, at pH 4.0 and 37°C

proof_lines: recombinant proenzyme without plant-specific insert domain, at pH 4.0 and 37°C

enzyme: phytepsin


enzyme_display_name: intramembrane prenyl-peptidase Rce1

enzyme: intramembrane prenyl-peptidase Rce1


substrate_name: acetyl-L-Cys(farnesyl)VIS

substrate: acetyl-L-Cys(farnesyl)VIS


enzyme_display_name: phosphonopyruvate decarboxylase

organism: Streptomyces viridochromogenes

proof_lines: mutant enzyme S25D, at pH 8.0 (HEPES buffer) and at 30°C

enzyme: phosphonopyruvate decarboxylase


proof_lines: mutant enzyme D297N, at pH 8.0 (HEPES buffer) and at 30°C


organism: Saccharomyces cerevisiae

proof_lines:
at pH 7.5 and 30°C
at pH 7.5 and 35°C


substrate_name: N-palmitoylethanolamine

proof_lines: recombinant enzyme, pH not specified in the publication, 37°C

proof_lines: pH 4.6, temperature not specified in the publication

substrate: N-palmitoylethanolamine


proof_lines: pH 4.5, 37°C, recombinant His6-tagged enzyme


enzyme_display_name: acetylputrescine deacetylase

organism: Marinobacter subterrani

proof_lines: pH 7.75, 25°C, in presence of 10 mM KCl, recombinant enzyme

proof_lines: pH 7.75, 25°C, in absence of KCl, recombinant enzyme

enzyme: acetylputrescine deacetylase


enzyme_display_name: reticuline oxidase

organism: Unknown

substrate_name: (S)-reticuline

enzyme: reticuline oxidase

substrate: (S)-reticuline


substrate_name: tetrachloroethene

substrate: tetrachloroethene


enzyme_display_name: gastricsin

substrate_name: acetyl-Tyr-Leu-Val-His methyl ester

enzyme: gastricsin

substrate: acetyl-Tyr-Leu-Val-His methyl ester


organism: Aphanothece halophytica

substrate_name: S-adenosyl-L-methionine

proof_lines: pH 8.8, 37°C, recombinant wild-type enzyme, with dimethylglycine

proof_lines: pH 8.8, 37°C, recombinant wild-type enzyme, with sarcosine

substrate: S-adenosyl-L-methionine


proof_lines: pH 8.8, 37°C, recombinant mutant P171Q, with dimethylglycine


organism: Methanohalophilus portucalensis

substrate_name: sarcosine

proof_lines: pH 7.3, 37°C, recombinant enzyme, with sarcosine

proof_lines: pH 7.3, 37°C, recombinant enzyme, with N,N-dimethylglycine

substrate: sarcosine


organism: Pseudomonas aeruginosa

substrate_name: Acetylcadaverine

substrate: Acetylcadaverine


enzyme_display_name: L-tryptophan decarboxylase

organism: Gelatoporia subvermispora

enzyme: L-tryptophan decarboxylase


proof_lines: recombinant wild-type enzyme, pH 7.5, 37°C


organism: Bacteroides fragilis

proof_lines: 25°C, pH 7.3, 5 mM MgCl2, 1 mM MnCl2, 0.2 mM thiamine diphosphate

proof_lines: 25°C, pH 7.3, 5 mM MgCl2, 1 mM MnCl2, 0.2 mM thiamine pyrophosphate


proof_lines: mutant enzyme S25N, at pH 8.0 (HEPES buffer) and at 30°C


proof_lines: mutant enzyme H111A, at pH 8.0 (HEPES buffer) and at 30°C


proof_lines: wild type enzyme, at pH 8.0 (HEPES buffer) and at 30°C


proof_lines: mutant enzyme D297E, at pH 8.0 (HEPES buffer) and at 30°C


enzyme_display_name: peptidyl-dipeptidase Dcp

organism: Escherichia coli

enzyme: peptidyl-dipeptidase Dcp


enzyme_display_name: Oplophorus-luciferin 2-monooxygenase

organism: Oplophorus gracilirostris

proof_lines: pH and temperature not specified in the publication

enzyme: Oplophorus-luciferin 2-monooxygenase


enzyme_display_name: butane monooxygenase (soluble)

organism: Thauera butanivorans

substrate_name: pentane

smiles: CCCCC

enzyme: butane monooxygenase (soluble)

substrate: pentane


substrate_name: propane

proof_lines:
product propan-1-ol, pH 7.2, 25°C
pH 7.2, 25°C
product pentan-2-ol, pH 7.2, 25°C

substrate: propane


smiles: CCC


organism: Mus musculus

substrate_name: 4-Nitrocatechol sulfate; H2O

substrate: 4-Nitrocatechol sulfate; H2O


enzyme_display_name: beta-primeverosidase

organism: Camellia sinensis

substrate_name: 2-phenylethyl beta-vicianoside

proof_lines: recombinant His-tagged enzyme, pH and temperature not specified in the publication

enzyme: beta-primeverosidase

substrate: 2-phenylethyl beta-vicianoside


enzyme_display_name: DNA-3-methyladenine glycosylase II

organism: Bacillus subtilis

substrate_name: 1,N6-ethenoadenine containing DNA; H2O

enzyme: DNA-3-methyladenine glycosylase II

substrate: 1,N6-ethenoadenine containing DNA; H2O


organism: Homo sapiens


enzyme_display_name: protein-fructosamine 3-kinase

enzyme: protein-fructosamine 3-kinase


enzyme_display_name: UDP-N-acetylglucosamine kinase

organism: Neisseria gonorrhoeae

substrate_name: ATP

assay_conditions_summary: pH 6; 298.15 K; cofactor ATP

enzyme: UDP-N-acetylglucosamine kinase

substrate: ATP


substrate_name: UDP-muramic acid

proof_lines: with UDP-muramic acid, pH 6.0, 25°C, recombinant wild-type enzyme ngzeta_1

proof_lines: with UDP-alpha-D-glucose, pH 6.0, 25°C, recombinant wild-type enzyme ngzeta_1

substrate: UDP-muramic acid


enzyme_display_name: muramoylpentapeptide carboxypeptidase

proof_lines: pH not specified in the publication, temperature not specified in the publication

enzyme: muramoylpentapeptide carboxypeptidase


enzyme_display_name: kallikrein 13

enzyme: kallikrein 13


substrate_name: UDP-N-acetyl-alpha-D-glucosamine

substrate: UDP-N-acetyl-alpha-D-glucosamine


substrate_name: UDP-alpha-D-glucose

substrate: UDP-alpha-D-glucose


substrate_name: H2O; 4-Nitrocatechol sulfate

substrate: H2O; 4-Nitrocatechol sulfate


enzyme_display_name: acyl-homoserine-lactone acylase

organism: Komagataeibacter europaeus

substrate_name: N-3-oxododecanoyl-L-homoserine lactone

enzyme: acyl-homoserine-lactone acylase

substrate: N-3-oxododecanoyl-L-homoserine lactone


enzyme_display_name: trans-L-3-hydroxyproline dehydratase

organism: Colwellia psychrerythraea

enzyme: trans-L-3-hydroxyproline dehydratase


organism: Labrenzia aggregata


organism: Agrobacterium vitis


organism: Azospirillum brasilense


organism: Bacillus thuringiensis


enzyme_display_name: arginine dihydrolase

organism: Anabaena sp.

substrate_name: L-arginine

proof_lines: mutant enzyme R139A, at pH 8.0 and 25Â°C

enzyme: arginine dihydrolase

substrate: L-arginine


proof_lines: wild type full-length enzyme, at pH 8.0 and 25Â°C

proof_lines: wild type N-terminal domain, at pH 8.0 and 25Â°C


organism: Synechocystis sp. PCC 6803


organism: Agrobacterium tumefaciens


organism: Burkholderia ambifaria


organism: Burkholderia thailandensis


organism: Thauera butanivorans

substrate_name: methane

smiles: C

substrate: methane


substrate_name: butane

smiles: CCCC

substrate: butane


substrate_name: ethane

smiles: CC

substrate: ethane


enzyme_display_name: hydroxylamine reductase

organism: Campylobacter curvus

substrate_name: hydroxylamine

proof_lines: oxidation reaction, recombinant MBP-tagged mutant W434Y, pH 7.0, 37°C

proof_lines: reduction reaction, recombinant MBP-tagged mutant W434Y, pH 7.0, 37°C

enzyme: hydroxylamine reductase

substrate: hydroxylamine


organism: Brucella suis biovar 1


sequence_source: UniProtKB:A7GXH2 REST accession lookup on 2026-07-08; sequence not emitted by exact-accession worker

proof_lines: oxidation reaction, recombinant MBP-tagged wild-type enzyme, pH 7.0, 37°C

proof_lines: reduction reaction, recombinant MBP-tagged wild-type enzyme, pH 7.0, 37°C


enzyme_display_name: glycine/sarcosine N-methyltransferase

organism: Aphanothece halophytica

substrate_name: S-adenosyl-L-methionine

sequence_source: UniProtKB:Q83WC4 REST accession lookup on 2026-07-08; sequence not emitted by exact-accession worker

proof_lines: pH 8.8, 37°C, recombinant wild-type enzyme, with sarcosine

proof_lines: pH 8.8, 37°C, recombinant wild-type enzyme, with glycine

enzyme: glycine/sarcosine N-methyltransferase

substrate: S-adenosyl-L-methionine


substrate_name: glycine

sequence_source: UniProtKB:Q83WC4 REST accession lookup on 2026-07-08; canonical identity only, R169K variant sequence not reconstructed

proof_lines: pH 8.8, 37°C, recombinant mutant R169K, with sarcosine

proof_lines: pH 8.8, 37°C, recombinant mutant R169K, with glycine

substrate: glycine


organism: Methanohalophilus portucalensis

sequence_source: UniProtKB:F6KV61 REST accession lookup on 2026-07-08; sequence not emitted by exact-accession worker

proof_lines: pH 7.3, 37°C, recombinant enzyme, with sarcosine

proof_lines: pH 7.3, 37°C, recombinant enzyme, with glycine


enzyme_display_name: glycerone-phosphate O-acyltransferase

organism: Saccharomyces cerevisiae

assay_conditions_summary: cofactor CoA

enzyme: glycerone-phosphate O-acyltransferase


enzyme_display_name: E2 ubiquitin-conjugating enzyme

organism: Homo sapiens

substrate_name: [ubiquitin carrier protein Ubc4]-L-cysteine

enzyme: E2 ubiquitin-conjugating enzyme


proof_lines: about, pH 7.5, temperature not specified in the publication


proof_lines: presence of the U-box domain of E3 enzyme CHIP, 37°C, pH not specified in the publication


enzyme_display_name: IMP-specific 5'-nucleotidase

organism: Plasmodium falciparum

substrate_name: IMP

proof_lines: pH 8, temperature not specified in the publication, wild-type enzyme

proof_lines: pH 5, temperature not specified in the publication, wild-type enzyme

enzyme: IMP-specific 5'-nucleotidase

substrate: IMP


enzyme_display_name: diacylglycerol diphosphate phosphatase

substrate_name: diacylglycerol diphosphate

enzyme: diacylglycerol diphosphate phosphatase

substrate: diacylglycerol diphosphate


organism: Labrenzia aggregata


organism: Micromonospora viridifaciens


substrate_name: Dolichyl phosphate; UDPglucose

substrate: Dolichyl phosphate; UDPglucose


organism: Bellis perennis

substrate_name: UDP-D-glucuronate

assay_conditions_summary: pH 7

proof_lines: pH 7.0, cosubstrate: cyanidin-3-O-(6-O-malonyl-beta-D-glucoside), native enzyme

proof_lines: pH 7.0, cosubstrate: cyanidin-3-O-(6-O-malonyl-beta-D-glucoside), recombinant enzyme

substrate: UDP-D-glucuronate


enzyme_display_name: enterobactin C-glucosyltransferase

organism: Escherichia coli O6:H1

enzyme: enterobactin C-glucosyltransferase


substrate_name: CMP-N-acetylneuraminate

substrate: CMP-N-acetylneuraminate


substrate_name: ganglioside GM3

substrate: ganglioside GM3


proof_lines: 37°C, pH not specified in the publication


substrate_name: [histone H3 peptide 1-15]-L-lysine9

proof_lines: substrate [histone H3 peptide 1-15]-N6-methyl-L-lysine9, SETDB1:ATF7IP complex, pH 7.6, 23°C

substrate: [histone H3 peptide 1-15]-L-lysine9


enzyme_display_name: polysialic-acid O-acetyltransferase

organism: Neisseria meningitidis

substrate_name: acetyl-CoA

enzyme: polysialic-acid O-acetyltransferase

substrate: acetyl-CoA


organism: Rattus norvegicus

substrate_name: Glycerone phosphate; Palmitoyl-CoA

substrate: Glycerone phosphate; Palmitoyl-CoA


organism: Rhizobium meliloti


organism: Shewanella pealeana


enzyme_display_name: selenate reductase (cytochrome c)

organism: Unknown

substrate_name: selenate

enzyme: selenate reductase (cytochrome c)

substrate: selenate


organism: Shewanella woodyi


enzyme_display_name: 4-oxalmesaconate hydratase

organism: Sphingomonas paucimobilis

enzyme: 4-oxalmesaconate hydratase


enzyme_display_name: acetylesterase

organism: Trichoderma reesei

substrate_name: 4-nitrophenyl acetate

proof_lines: purified recombinant enzyme, 30°C, pH 5.8

enzyme: acetylesterase

substrate: 4-nitrophenyl acetate


organism: Drosophila melanogaster

substrate_name: Unknown

substrate: Unknown


organism: Escherichia coli

substrate_name: tributyrin

assay_conditions_summary: pH 7.1; 303.15 K; buffer 20 mM phosphate

proof_lines: mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C

substrate: tributyrin


substrate_name: 4-nitrophenyl butyrate

proof_lines: wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C

substrate: 4-nitrophenyl butyrate


proof_lines: mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C


organism: Acetivibrio thermocellus

assay_conditions_summary: pH 6; 313.15 K; buffer 0.1 M sodium phosphate

proof_lines: isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C


organism: Cellvibrio japonicus

proof_lines: isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C

proof_lines: isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C


organism: Marchantia polymorpha

substrate_name: 2-t-butylcyclohex-1-en-1-yl acetate

proof_lines:
isoenzyme Est I, pH 7.0, 35°C
isoenzyme Est II, pH 7.0, 35°C

substrate: 2-t-butylcyclohex-1-en-1-yl acetate


organism: Helicobacter pylori

substrate_name: H2O; p-Nitrophenyl acetate

substrate: H2O; p-Nitrophenyl acetate


substrate_name: p-Nitrophenyl acetate

substrate: p-Nitrophenyl acetate


organism: Homo sapiens

substrate_name: p-Nitrophenyl acetate; H2O

substrate: p-Nitrophenyl acetate; H2O


organism: Mycobacterium tuberculosis


proof_lines: mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C


proof_lines: mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C


organism: Alicyclobacillus tolerans


enzyme_display_name: lytic cellulose monooxygenase (C4-dehydrogenating)

organism: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)


enzyme_display_name: arsenate reductase (donor)

organism: Chrysiogenes arsenatis

substrate_name: arsenate

enzyme: arsenate reductase (donor)

substrate: arsenate


organism: Desulfitobacterium hafniense

substrate_name: tetrachloroethene (with reduced methyl viologen as electron donor)


organism: Desulfitobacterium hafniense (strain Y51)

substrate_name: tetrachloroethene

substrate: tetrachloroethene


organism: Sulfurospirillum multivorans

substrate: tetrachloroethene (with reduced methyl viologen as electron donor)


organism: Escherichia coli (strain K12)

substrate_name: hydroxylamine (at pH 9)

assay_conditions_summary: pH 9

substrate: hydroxylamine (at pH 9)


enzyme_display_name: aliphatic sulfonate oxidoreductase

organism: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

substrate_name: taurine

enzyme: aliphatic sulfonate oxidoreductase

substrate: taurine


organism: Aphanothece halophytica

substrate_name: S-adenosyl-L-homocysteine (at pH 8.8 and at 37 degrees Celsius)


organism: Nicotiana tabacum


organism: Pantoea dispersa


organism: Phocaeicola vulgatus


organism: Mus musculus

substrate_name: histone octamer

substrate: histone octamer


enzyme_display_name: 2-phosphinomethylmalate synthase

organism: Streptomyces hygroscopicus

substrate_name: acetyl-CoA

enzyme: 2-phosphinomethylmalate synthase

substrate: acetyl-CoA


organism: Bellis perennis

substrate_name: cyanidin 3-O-6''-O-malonylglucoside

substrate: cyanidin 3-O-6''-O-malonylglucoside


enzyme_display_name: oleanolate 3-O-glucosyltransferase

organism: Barbarea vulgaris

substrate_name: UDP-glucose

enzyme: oleanolate 3-O-glucosyltransferase

substrate: UDP-glucose


organism: Homo sapiens

substrate_name: CMP-N-acetylneuraminate

substrate: CMP-N-acetylneuraminate


substrate_name: CMP-N-acetyl-beta-neuraminate

substrate: CMP-N-acetyl-beta-neuraminate


substrate_name: N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucose (with a constant CMP-Neu5Ac concentration of 1 mM)


substrate_name: ganglioside GM3

substrate: ganglioside GM3


substrate_name: a neolactoside IV3-alpha-NeuAc-nLc4Cer

substrate: a neolactoside IV3-alpha-NeuAc-nLc4Cer


enzyme_display_name: protein-fructosamine 3-kinase

substrate_name: deoxymorpholinofructose

enzyme: protein-fructosamine 3-kinase

substrate: deoxymorpholinofructose


substrate_name: adenylyl

assay_conditions_summary: cofactor ATP

substrate: adenylyl


substrate_name: p-nitrophenyl acetate

substrate: p-nitrophenyl acetate


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: pNP-acetate

substrate: pNP-acetate


organism: Trichoderma reesei

substrate_name: 4-nitrophenyl acetate

substrate: 4-nitrophenyl acetate


enzyme_display_name: Aspergillus nuclease S1

organism: Aspergillus oryzae (strain ATCC 42149 / RIB 40)

substrate_name: ssDNA

enzyme: Aspergillus nuclease S1

substrate: ssDNA


organism: Arabidopsis thaliana


substrate_name: PtdIns(4,5)P2 (at pH 7.0 and 22 degrees Celsius)


substrate_name: phosphatidylinositol 4,5-bisphosphate

substrate: phosphatidylinositol 4,5-bisphosphate


organism: Rattus norvegicus


substrate_name: 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate (at pH 6.5 and 37 degrees Celsius)


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: Unknown

substrate: Unknown


enzyme_display_name: L-tryptophan decarboxylase

organism: Psilocybe cubensis

substrate_name: L-phenylalanine

enzyme: L-tryptophan decarboxylase

substrate: L-phenylalanine


substrate_name: L-tryptophan

substrate: L-tryptophan


enzyme_display_name: trans-L-3-hydroxyproline dehydratase

enzyme: trans-L-3-hydroxyproline dehydratase


organism: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)


enzyme_display_name: grixazone synthase

organism: Streptomyces griseus subsp. griseus (strain JCM 4626 / CBS 651.72 / NBRC 13350 / KCC S-0626 / ISP 5235)

substrate_name: alpha-aminophenol

proof_lines: kcat is 20 sec(-1) with alpha-aminophenol as substrate. kcat is 14 sec(-1) with 3,4-AHBAL as substrate. kcat is 18 sec(-1) with 2-amino-4-methylphenol as substrate. kcat is 0.8 sec(-1) with 3,4-dihydroxybenzaldehyde as substrate. kcat is 12

enzyme: grixazone synthase

substrate: alpha-aminophenol


enzyme_display_name: 1-hydroxy-2-naphthoate 1,2-dioxygenase

organism: Nocardioides sp. (strain KP7)

proof_lines: kcat is 114 sec(-1) for 1-hydroxy-2-naphthoate.

enzyme: 1-hydroxy-2-naphthoate 1,2-dioxygenase


enzyme_display_name: ammonia monooxygenase

organism: Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)

proof_lines: kcat is 20 sec(-1) for ammonia as substrate.

enzyme: ammonia monooxygenase


organism: Shewanella sp. (strain ANA-3)

substrate_name: arsenate

substrate: arsenate


organism: Methanosarcina thermophila (strain ATCC 43570 / DSM 1825 / OCM 12 / VKM B-1830 / TM-1)

substrate_name: reduced 2-hydroxyphenazine

proof_lines: kcat is 74 sec(-1) at 25 degrees Celsius.

substrate: reduced 2-hydroxyphenazine


organism: Thauera selenatis

substrate_name: selenate (with reduced benzyl viologen as the electron donor)

proof_lines: kcat is 387 sec(-1) (with reduced benzyl viologen as the electron donor).


substrate: selenate (with reduced benzyl viologen as the electron donor)


substrate_name: tRNA(Gly GCC)

proof_lines: kcat is 0.54 min(-1) for tRNA(Gly GCC) and 0.31 min(-1) for tRNA(Val UAC) (m1G9)-methylation, respectively.

substrate: tRNA(Gly GCC)


organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843)

substrate_name: tRNA(Gly)

proof_lines: kcat is 0.16 min(-1) for tRNA(Gly) (m1G9)-methylation.

substrate: tRNA(Gly)


substrate_name: N-palmitoylethanolamine

substrate: N-palmitoylethanolamine


enzyme_display_name: acetylputrescine deacetylase

organism: Danio rerio

substrate_name: acetylcadaverine

enzyme: acetylputrescine deacetylase

substrate: acetylcadaverine


organism: Homo sapiens


organism: Mycoplana ramosa

substrate_name: N-acetylcadaverine

substrate: N-acetylcadaverine


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)


enzyme_display_name: acyl-homoserine-lactone acylase

organism: Komagataeibacter europaeus

substrate_name: 3-oxo-decanoyl homoserine lactone

enzyme: acyl-homoserine-lactone acylase

substrate: 3-oxo-decanoyl homoserine lactone


enzyme_display_name: 6-aminohexanoate-cyclic-dimer hydrolase

organism: Paenarthrobacter ureafaciens

enzyme: 6-aminohexanoate-cyclic-dimer hydrolase


enzyme_display_name: diacylglycerol diphosphate phosphatase

organism: Escherichia coli (strain K12)

substrate_name: undecaprenyl pyrophosphate

enzyme: diacylglycerol diphosphate phosphatase

substrate: undecaprenyl pyrophosphate


enzyme_display_name: galactolipase

organism: Parachlorella kessleri

proof_lines:
pH 6.5, 37°C, recombinant enzyme
pH 6.5, 37°C, native enzyme

enzyme: galactolipase


organism: Arabidopsis thaliana

substrate_name: ssDNA (at pH 7.0 and 37 degrees Celsius)

proof_lines: kcat is 19171 min(-1) with ssDNA as substrate (at pH 7.0 and 37 degrees Celsius).


proof_lines: kcat is 4.8 sec(-1) with 1D-myo-inositol 1,4,5-trisphosphate.


proof_lines: kcat is 0.026 sec(-1) with 1D-myo-inositol 1,4,5-trisphosphate.


proof_lines: kcat is 26 sec(-1) with 1D-myo-inositol 1,4,5-trisphosphate.


enzyme_display_name: IMP-specific 5'-nucleotidase

organism: Plasmodium falciparum (isolate 3D7)

substrate_name: IMP (at pH 5, 25 degrees Celsius and in the absence of ATP)

assay_conditions_summary: pH 5; cofactor ATP

proof_lines: kcat is 10.3 sec(-1) with IMP as substrate (at pH 5, 25 degrees Celsius and in the absence of ATP) (PubMed:32591529). kcat is 13.5 sec(-1) with IMP as substrate (at pH 8, 25 degrees Celsius and in the absence of ATP) (PubMed:32591529). kcat

enzyme: IMP-specific 5'-nucleotidase


enzyme_display_name: (R)-specific secondary-alkylsulfatase (type III)

organism: Pseudomonas sp

substrate_name: (R)-2-octyl sulfate

proof_lines: kcat is 262 min(-1) with (R)-2-octyl sulfate as substrate. kcat is 52 min(-1) with 1-octyl sulfate as substrate.

substrate: (R)-2-octyl sulfate


enzyme_display_name: inosine nucleosidase

substrate_name: uridine

proof_lines: kcat is 2.65 sec(-1) with xanthosine as substrate (PubMed:30787180). kcat is 33.5 sec(-1) with uridine as substrate (PubMed:30787180). kcat is 23.3 sec(-1) with xanthosine as substrate (when in complex with URH2) (PubMed:30787180). kcat is

enzyme: inosine nucleosidase

substrate: uridine


substrate_name: xanthosine (when in complex with URH2)

proof_lines: kcat is 23.3 sec(-1) with xanthosine as substrate (when in complex with URH2) (PubMed:30787180). kcat is 55.3 sec(-1) with uridine as substrate (when in complex with URH2) (PubMed:30787180). kcat is 42.3 sec(-1) with inosine as substrate (w

substrate: xanthosine (when in complex with URH2)


organism: Caenorhabditis elegans

substrate_name: inosine (at pH 7.0 and 37 degrees Celsius)

proof_lines: kcat is 0.79 sec(-1) with inosine as substrate (at pH 7.0 and 37 degrees Celsius) (PubMed:12777783). kcat is 0.203 sec(-1) with inosine as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:28218438). kcat is 0.78 sec(-1) with adenosine a


organism: Crithidia fasciculata

substrate_name: inosine

proof_lines: kcat is 32 sec(-1) with inosine as substrate. kcat is 243 sec(-1) with 5-methyluridine as substrate.

substrate: inosine


organism: Leishmania major

proof_lines: kcat is 119 sec(-1) with inosine as substrate. kcat is 32 sec(-1) with uridine as substrate. kcat is 0.6 sec(-1) with guanosine as substrate. kcat is 0.6 sec(-1) with adenosine as substrate. kcat is 0.4 sec(-1) with cytidine as substrate.


enzyme_display_name: caricain

organism: Carica papaya

proof_lines: kcat is 0.5 sec(-1) with Boc-Ala-Ala-Gly-NHPhNO(2) as substrate (PubMed:2404797). kcat is 0.1 sec(-1) with Boc-Ala-Ala-Gly-NHMec as substrate (PubMed:2404797).

enzyme: caricain


organism: Bos taurus

substrate_name: IMP

substrate: IMP


organism: Gallus gallus

substrate_name: IMP | GMP

substrate: IMP | GMP


substrate_name: AMP | dCMP | IMP

substrate: AMP | dCMP | IMP


substrate_name: AMP | GMP | IMP | dTMP | UMP | CMP | dAMP | NMN


organism: Rattus norvegicus

substrate_name: IMP | dIMP | GMP | dGMP

substrate: IMP | dIMP | GMP | dGMP


organism: Homo sapiens

substrate_name: N-hexadecanoyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine (in the presence of deoxycholic acid) | N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-1,2-diacyl-sn-glycero-3-phosphoethanolamine (in the presence of deoxycholic acid)

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


substrate_name: inosine | adenosine

substrate: inosine | adenosine


enzyme_display_name: cathepsin X

substrate_name: Z-Phe-Arg-NMec

enzyme: cathepsin X

substrate: Z-Phe-Arg-NMec


enzyme_display_name: intramembrane prenyl-peptidase Rce1

substrate_name: farnesyl-Ki-Ras | geranylgeranyl-Ki-Ras

enzyme: intramembrane prenyl-peptidase Rce1

substrate: farnesyl-Ki-Ras | geranylgeranyl-Ki-Ras


substrate_name: N-palmitoylethanolamine

smiles: CCCCCCCCCCCCCCCC(=O)NCCO


enzyme_display_name: photosystem II

organism: Helianthus annuus

substrate_name: plastoquinol

proof_lines: at 21% O2, pH not specified in the publication, temperature not specified in the publication

enzyme: photosystem II

substrate: plastoquinol


organism: Spinacia oleracea

substrate_name: H2O; hn; Plastoquinone

substrate: H2O; hn; Plastoquinone


organism: Pseudaminobacter salicylatoxidans


proof_lines: pH 8.0, temperature not specified in the publication, mutant M46V


proof_lines: pH 8.0, temperature not specified in the publication, mutant W104Y


proof_lines: pH 8.0, temperature not specified in the publication, wild-type enzyme


proof_lines: pH 8.0, temperature not specified in the publication, mutant A85H


proof_lines: pH 8.0, temperature not specified in the publication, mutant L38Q


organism: Pseudomonas sp.

proof_lines:
competitive inhibition
substrate inhibition


enzyme_display_name: linolenate 9R-lipoxygenase

organism: Nostoc sp.

substrate_name: alpha-linolenate

enzyme: linolenate 9R-lipoxygenase

substrate: alpha-linolenate


enzyme_display_name: chymopapain

proof_lines: kcat is 0.5 sec(-1) with Boc-Ala-Ala-Gly-NHPhNO(2) as substrate (PubMed:2404797). kcat is 0.4 sec(-1) with Boc-Ala-Ala-Gly-NHMec as substrate (PubMed:2404797).

enzyme: chymopapain


organism: Methanococcus maripaludis (strain DSM 14266 / JCM 13030 / NBRC 101832 / S2 / LL)

substrate_name: peptide substrate DABCYL-ARSGAKASGC(farnesyl)LVS-EDANS where EDANS is 5-[(2-aminoethyl)amino]naphthalene-1-sulphonic acid fluorophore and DABCYL is 4-{[4-(dimethylamino)phenyl]azo}benzoic acid quencher

proof_lines: kcat is 0.175 sec(-1) for peptide substrate DABCYL-ARSGAKASGC(farnesyl)LVS-EDANS.


enzyme_display_name: N-acetylmuramoyl-L-alanine amidase

organism: Escherichia coli (strain K12)

enzyme: N-acetylmuramoyl-L-alanine amidase


enzyme_display_name: urethanase

organism: Agrobacterium tumefaciens

substrate_name: ethyl carbamate

proof_lines: kcat is 0.81 sec(-1) with ethyl carbamate as substrate.

enzyme: urethanase

substrate: ethyl carbamate


enzyme_display_name: arginine dihydrolase

organism: Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)

substrate_name: arginine

proof_lines: kcat is 271.1 min(-1) with arginine as substrate (for full-length AgrE) (PubMed:32198136). kcat is 292.6 min(-1) with arginine as substrate (for NTD-AgrE) (PubMed:32198136).

enzyme: arginine dihydrolase

substrate: arginine


organism: Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

proof_lines: kcat is 6.4 sec(-1) with arginine as substrate.


enzyme_display_name: L-tryptophan decarboxylase

organism: Clostridium sporogenes (strain ATCC 15579)

substrate_name: tryptophan

proof_lines: kcat is 1200 min(-1) with tryptophan as substrate.

enzyme: L-tryptophan decarboxylase

substrate: tryptophan


organism: Mediterraneibacter gnavus (strain ATCC 29149 / DSM 114966 / JCM 6515 / VPI C7-9)

proof_lines: kcat is 4400 min(-1) with tryptophan as substrate. kcat is 230 min(-1) with phenylalanine as substrate.


organism: Pseudomonas fluorescens

substrate_name: ACMS in presence of Zn(2+)

proof_lines: kcat is 6.65 sec(-1) to 7.3 sec(-1) for ACMS decarboxylation in presence of Co(2+) (PubMed:16131206, PubMed:16716073, PubMed:16939194). kcat is 6.5 sec(-1) to 15.8 sec(-1) for ACMS decarboxylation in presence of Zn(2+) (PubMed:16716073, Pub

substrate: ACMS in presence of Zn(2+)


enzyme_display_name: trans-L-3-hydroxyproline dehydratase

organism: Agrobacterium fabrum (strain C58 / ATCC 33970)

proof_lines: kcat is 27 sec(-1) for t3LHyp dehydration. kcat is 0.40 sec(-1) for t4LHyp epimerization.

enzyme: trans-L-3-hydroxyproline dehydratase


organism: Allorhizobium ampelinum (strain ATCC BAA-846 / DSM 112012 / S4)

proof_lines: kcat is 4.3 sec(-1) for t3LHyp dehydration.


organism: Bacillus cereus (strain ATCC 10987 / NRS 248)

proof_lines: kcat is 1.2 sec(-1) for t4LHyp epimerization. The reaction of t3LHyp dehydration is too slow to measure kinetic parameters.


organism: Bacillus thuringiensis subsp. konkukian (strain 97-27)

proof_lines: kcat is 23 sec(-1) for t3LHyp dehydration. kcat is 0.16 sec(-1) for t4LHyp epimerization. The reaction of t4LHyp epimerization is too slow to measure KM for t4LHyp.


organism: Brucella suis biovar 1 (strain 1330)

proof_lines: kcat is 17 sec(-1) for t3LHyp dehydration.


organism: Burkholderia ambifaria (strain ATCC BAA-244 / DSM 16087 / CCUG 44356 / LMG 19182 / AMMD)

proof_lines: kcat is 43 sec(-1) for t3LHyp dehydration.


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

substrate_name: the dehydration of trans-3-hydroxy-L-proline


organism: Rhizobium meliloti (strain 1021)

proof_lines: kcat is 7.9 sec(-1) for t3LHyp dehydration. kcat is 0.089 sec(-1) for t4LHyp epimerization.


organism: Roseibium aggregatum (strain ATCC 25650 / DSM 13394 / JCM 20685 / NBRC 16684 / NCIMB 2208 / IAM 12614 / B1)

proof_lines: kcat is 15 sec(-1) for t3LHyp dehydration.


organism: Shewanella pealeana (strain ATCC 700345 / ANG-SQ1)

substrate_name: Unknown

proof_lines: kcat is 0.15 sec(-1) for t3LHyp dehydration. The reaction is too slow to measure KM for t3LHyp.

substrate: Unknown


organism: Shewanella woodyi (strain ATCC 51908 / MS32)

proof_lines: kcat is 4.1 sec(-1) for t3LHyp dehydration.


organism: Nostoc sp.

substrate_name: linoleate

substrate: linoleate


substrate_name: alpha-linolenic acid

substrate: alpha-linolenic acid


enzyme_display_name: Oplophorus-luciferin 2-monooxygenase

organism: Oplophorus gracilirostris

substrate_name: coelenterazine

proof_lines:
pH 7.6, native enzyme
pH 7.6, catalytic subunit 19kOLase
pH 7.6, native enzymee

enzyme: Oplophorus-luciferin 2-monooxygenase

substrate: coelenterazine


enzyme_display_name: ammonia monooxygenase

proof_lines: whole cell assay, pH not specified in the publication, temperature not specified in the publication

enzyme: ammonia monooxygenase


organism: Nitrosomonas europaea

substrate_name: ammonia

substrate: ammonia


enzyme_display_name: lytic cellulose monooxygenase (C4-dehydrogenating)

organism: Panus similis

substrate_name: cellotetraose

substrate: cellotetraose


organism: Talaromyces amestolkiae

proof_lines: variant lacking the C-terminal linker-like region, pH 6, 23°C

proof_lines: variant with an additional C-terminal carbohydrate binding domain, pH 6, 23°C

enzyme: lytic cellulose monooxygenase (C4-dehydrogenating)


enzyme_display_name: arsenate reductase (donor)

organism: Chrysiogenes arsenatis

substrate_name: arsenate

enzyme: arsenate reductase (donor)

substrate: arsenate


organism: Chlamydomonas reinhardtii

proof_lines: S0.5 value, pH 7.9, temperature not specified in the publication


substrate_name: 2-mercaptoethanol glutathione disulfide

proof_lines: cosubstrate 2-mercaptoethanol glutathione disulfide, pH not specified in the publication, temperature not specified in the publication

proof_lines: cosubstrate glutathione, S0.5 value, pH not specified in the publication, temperature not specified in the publication

substrate: 2-mercaptoethanol glutathione disulfide


substrate_name: glutathione

proof_lines: pH not specified in the publication, temperature not specified in the publication

proof_lines: pH 7.9, temperature not specified in the publication

substrate: glutathione


organism: Homo sapiens

substrate_name: Reduced glutathione; Glutathionylated RNase A


substrate: Reduced glutathione; Glutathionylated RNase A


substrate_name: Asn-Arg-Cys-Ser-Gln-Gly-Ser-Cys-Trp-Asn

substrate: Asn-Arg-Cys-Ser-Gln-Gly-Ser-Cys-Trp-Asn


enzyme_display_name: flavonol 7-O-beta-glucosyltransferase

organism: Arabidopsis thaliana

substrate_name: anthranilate

enzyme: flavonol 7-O-beta-glucosyltransferase

substrate: anthranilate


substrate_name: curcumin

substrate: curcumin


enzyme_display_name: acetylesterase

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: palmitoyl-CoA

proof_lines: kcat is 0.00342 sec(-1) with palmitoyl-CoA as substrate.

enzyme: acetylesterase

substrate: palmitoyl-CoA


substrate_name: Insulin

substrate: Insulin


organism: Mus musculus


organism: Populus sp.


proof_lines: mutant C87S, pH not specified in the publication, temperature not specified in the publication


substrate_name: glutathionylated BSA

proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication

substrate: glutathionylated BSA


organism: Rattus norvegicus

substrate_name: GSH

proof_lines:
enzyme from different rat tissues
enzymes from different rat tissues

substrate: GSH


organism: Methanomassiliicoccus luminyensis

substrate_name: coenzyme B-coenzyme M disulfide

substrate: coenzyme B-coenzyme M disulfide


organism: Methanosarcina acetivorans

substrate_name: coenzyme M

proof_lines: subunit HdrB2C2, at 21°C, pH not specified in the publication

proof_lines: subunit HdrB2, at 21°C, pH not specified in the publication

substrate: coenzyme M


organism: Methanosarcina barkeri

proof_lines: pH 7.0, 37°C, reaction with reduced benzyl viologen


organism: Methanosarcina thermophila

substrate_name: dihydromethanophenazine

substrate: dihydromethanophenazine


organism: Methanothermobacter thermautotrophicus

proof_lines: pH 7, 65°C, H2: heterodisulfide oxidoreductase complex


enzyme_display_name: selenate reductase (cytochrome c)

organism: Alcaligenes sp.

substrate_name: selenate

enzyme: selenate reductase (cytochrome c)

substrate: selenate


organism: Desulfitobacterium hafniense

substrate_name: methyl viologen

proof_lines: at a tetrachloroethene concentration of 1 mM

substrate: methyl viologen


organism: Desulfitobacterium sp.

substrate_name: hexachloroethane

substrate: hexachloroethane


organism: Paraclostridium bifermentans

substrate_name: tetrachloroethene

substrate: tetrachloroethene


organism: Sulfurospirillum multivorans

proof_lines: titanium(III)citrate as sole electron donor

proof_lines: methyl viologen as artificial electron donor

proof_lines: reduced 1.6 mM methyl viologen as electron donor


enzyme_display_name: hydroxylamine reductase

organism: Caminibacter mediatlanticus

substrate_name: hydroxylamine

proof_lines: oxidation reaction, recombinant MBP-tagged wild-type enzyme, pH 7.0, 37°C

proof_lines: reduction reaction, recombinant MBP-tagged wild-type enzyme, pH 7.0, 37°C

enzyme: hydroxylamine reductase

substrate: hydroxylamine


substrate_name: nitrite

proof_lines: reduction reaction, recombinant MBP-tagged mutant W434Y, pH 7.0, 37°C

substrate: nitrite


organism: Campylobacter fetus subsp. fetus


organism: Cucurbita pepo

substrate_name: reduced benzyl viologen

substrate: reduced benzyl viologen


organism: Escherichia coli

proof_lines:
at pH 9
at pH 7.5


organism: Pyrococcus furiosus


organism: Vigna radiata var. radiata

substrate_name: dolichyl phosphate

substrate: dolichyl phosphate


organism: Zea mays

substrate_name: UDP-glucose

substrate: UDP-glucose


enzyme_display_name: mannosyl-3-phosphoglycerate synthase

organism: Dehalococcoides mccartyi

enzyme: mannosyl-3-phosphoglycerate synthase


organism: Pyrococcus horikoshii

substrate_name: GDP-mannose

substrate: GDP-mannose


organism: Rhodothermus marinus


organism: Rubrobacter xylanophilus

substrate_name: GDP-glucose

substrate: GDP-glucose


organism: Thermus thermophilus


organism: Beta vulgaris

substrate_name: betanidin

substrate: betanidin


organism: Euonymus alatus

proof_lines:
pH 7.5, 30°C, cosubstrate kaempferol
pH 7.5, 30°C


organism: Glebionis segetum

substrate_name: quercetin

substrate: quercetin


organism: Nicotiana tabacum


organism: Paederia scandens

substrate_name: kaempferol

proof_lines:
pH 7.5
pH 7.5, cosubstrate kaempferol

substrate: kaempferol


organism: Mus musculus

substrate_name: histone K4-acetylK9

substrate: histone K4-acetylK9


organism: Neurospora crassa

substrate_name: biotinyl-MARTKQTARKSTGGKAPRKQ

assay_conditions_summary: pH 9.8; 295.15 K; buffer containing 50 mM glycine (pH 9

proof_lines: in methylation buffer containing 50 mM glycine (pH 9.8), 2 mM dithiothreitol, 0.025 mg/ml bovine serum albumin, 10% (v/v) glycerol, at 22°C

substrate: biotinyl-MARTKQTARKSTGGKAPRKQ


enzyme_display_name: 1-deoxy-D-xylulose-5-phosphate synthase

substrate_name: DXP synthase; D-Glyceraldehyde 3-phosphate

proof_lines:
pH 8.0, 37°C
Ki above 0.2 mM, at pH 8.0 and 37°C
Ki above 0.1 mM, at pH 8.0 and 37°C

enzyme: 1-deoxy-D-xylulose-5-phosphate synthase


enzyme_display_name: polysialic-acid O-acetyltransferase

substrate_name: acetyl-CoA

enzyme: polysialic-acid O-acetyltransferase

substrate: acetyl-CoA


proof_lines: mutant NeuO + 24 hepta-nucleotide repeats

proof_lines: mutant NeuO + 12 hepta-nucleotide repeats

proof_lines: mutant NeuO + 36 hepta-nucleotide repeats


enzyme_display_name: meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase I

organism: Mycobacterium tuberculosis

substrate_name: malonyl-[acyl-carrier protein from Escherichia coli]

proof_lines: pH and temperature not specified in the publication


enzyme_display_name: meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase II

organism: Escherichia coli

substrate_name: malonyl-CoA

assay_conditions_summary: pH 8.5; 298.15 K; cofactor CoA

substrate: malonyl-CoA


substrate_name: malonyl-phosphopantetheine 8-mer

enzyme: meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase II

substrate: malonyl-phosphopantetheine 8-mer


substrate_name: C20:0-[acyl-carrier protein from Escherichia coli]


enzyme_display_name: glycerone-phosphate O-acyltransferase

organism: Cavia porcellus

substrate_name: dihydroxyacetone phosphate

assay_conditions_summary: pH 7.4; cofactor CoA

proof_lines:
purified enzyme, pH 7.4
pH 5.5
high concentration of substrate

enzyme: glycerone-phosphate O-acyltransferase

substrate: dihydroxyacetone phosphate


organism: Leishmania major


organism: Oryctolagus cuniculus


organism: Rattus norvegicus

substrate_name: Unknown

substrate: Unknown


organism: Saccharomyces cerevisiae


enzyme_display_name: E2 ubiquitin-conjugating enzyme

organism: Homo sapiens

proof_lines: mutant D576A, pH 7.5, temperature not specified in the publication

enzyme: E2 ubiquitin-conjugating enzyme


proof_lines: mutant D576E, pH 7.5, temperature not specified in the publication


proof_lines: mutant K528A, pH 7.5, temperature not specified in the publication


proof_lines: wild-type, pH 7.5, temperature not specified in the publication


proof_lines: mutant D576N, pH 7.5, temperature not specified in the publication


organism: Acanthamoeba castellanii

substrate_name: dolichyl phosphate


organism: Candida albicans

substrate_name: UDP-glucose


substrate_name: arsenate

proof_lines:
pH 8.5, 30°C, 2 mM glutathione
pH 8.5, 30°C, 5 mM glutathione

substrate: arsenate


organism: Hydrogenophaga sp. NT14

substrate_name: arsenite

proof_lines: pH 5.5, calculated with an Mr of 309000 Da

substrate: arsenite


organism: Hydrogenophaga sp. NT-26


organism: Pyrobaculum aerophilum

proof_lines: isoform Arr-Mo, with methyl viologen as acceptor, at pH 7.5 and 80°C

proof_lines: isoform Arr-W, with methyl viologen as acceptor, at pH 7.5 and 80°C


enzyme_display_name: reticuline oxidase

organism: Arabidopsis thaliana

substrate_name: cellotetraose

enzyme: reticuline oxidase

substrate: cellotetraose


organism: Berberis beaniana

substrate_name: (S)-reticuline

substrate: (S)-reticuline


organism: Eschscholzia californica

sequence_variant_note: Resolved canonical sequence from UniProt P30986 plus row-specific mutation reconstruction and applied mutation signature C166A to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 9; 310.15 K; cofactor FAD


sequence_variant_note: Resolved canonical sequence from UniProt P30986 plus row-specific mutation reconstruction and applied mutation signature Y106F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt P30986 plus row-specific mutation reconstruction and applied mutation signature E417Q to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt P30986 plus row-specific mutation reconstruction and applied mutation signature H459A to reconstruct the assayed variant sequence.


sequence_source: UniProt P30986 PMID-linked direct lookup

proof_lines: pH 9.0, 37°C, recombinant wild-type enzyme


proof_lines: All kinetic experiments are done with 10 mm Tris-HCl, 0.01% Triton X-100, and 10 mm DTT and at pH 8.5.


organism: Escherichia coli O1:K1 / APEC

proof_lines: kcat is 0.51 sec(-1) with acetyl-CoA as substrate. kcat is 1.99 sec(-1) with polymer of sialic acid as substrate.


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: hexadecanoyl-[ACP]

proof_lines: kcat is 5.3 min(-1) with hexadecanoyl-[ACP] as substrate. kcat is 4.5 min(-1) with eicosanoyl-[ACP] as substrate. kcat is 4.8 min(-1) with malonyl-[ACP] as substrate (PubMed:11600501). kcat is 21 min(-1) with malonyl-CoA as substrate. kcat

enzyme: meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase I

substrate: hexadecanoyl-[ACP]


proof_lines: kcat is 1.6 min(-1) with hexadecanoyl-[ACP] as substrate. kcat is 0.6 min(-1) with eicosanoyl-[ACP] as substrate. kcat is 1.4 min(-1) with malonyl-[ACP] as substrate.


substrate_name: beta-catenin-monoubiquitin

proof_lines: Measured for E2-E3 complex composed of neddylated UBE2R1 and the CRL2(FEM1C) complex, using an 'Arg-48' donor ubiquitin.

substrate: beta-catenin-monoubiquitin


substrate_name: acceptor ubiquitin

proof_lines: Measured for E2-E3 complex composed of neddylated UBE2R2 and the CRL2(FEM1C) complex, using an 'Arg-48' donor ubiquitin.

substrate: acceptor ubiquitin


enzyme_display_name: oleanolate 3-O-glucosyltransferase

organism: Barbarea vulgaris

substrate_name: oleanolate

proof_lines: kcat is 0.816 sec(-1) with oleanolate as substrate (PubMed:23027665). kcat is 0.389 sec(-1) with hederagenin as substrate (PubMed:23027665).

enzyme: oleanolate 3-O-glucosyltransferase

substrate: oleanolate


proof_lines: kcat is 0.023 sec(-1) with oleanolate as substrate (PubMed:23027665). kcat is 0.006 sec(-1) with hederagenin as substrate (PubMed:23027665).


enzyme_display_name: enterobactin C-glucosyltransferase

organism: Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

substrate_name: enterobactin

proof_lines: kcat is 11.2 min(-1) with enterobactin as substrate. kcat is 9.7 min(-1) with UDP-Glc as substrate (for the first C-glucosylation).

enzyme: enterobactin C-glucosyltransferase

substrate: enterobactin


enzyme_display_name: corrinoid adenosyltransferase EutT

organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

substrate_name: ATP

enzyme: corrinoid adenosyltransferase EutT

substrate: ATP


enzyme_display_name: 5-aminovalerate transaminase

organism: Escherichia coli (strain K12)

substrate_name: 4-aminobutanoate (measured for a coupled GabT/GabD reaction)

proof_lines: kcat is 47.4 sec(-1) with 4-aminobutanoate as substrate (at 25 degrees Celsius and pH 7.8).

enzyme: 5-aminovalerate transaminase


enzyme_display_name: gentamicin 2''-nucleotidyltransferase

organism: Klebsiella pneumoniae

substrate_name: kanamycin B

proof_lines: kcat is 15.3 sec(-1) for ATP and 5.2 sec(-1) for kanamycin B.

enzyme: gentamicin 2''-nucleotidyltransferase

substrate: kanamycin B


organism: Alcaligenes sp. CKCr-6A

substrate_name: selenate

substrate: selenate


organism: Enterobacter cloacae

proof_lines:
using electron donormethyl viologen
20°C
with reduced benzyl viologen, 20°C


organism: Sulfurospirillum barnesii


organism: Thauera selenatis

assay_conditions_summary: pH 6

proof_lines: tungsten-SER, pH and temperature not specified in the publication, Vmax: 0.01 micromol/min/mg

proof_lines: molybdenum-SER, pH and temperature not specified in the publication, Vmax: 0.2 micromol/min/mg


enzyme_display_name: glycine/sarcosine N-methyltransferase

organism: Aphanothece halophytica

substrate_name: N-methylglycine

sequence_source: UniProtKB:Q83WC4 (PMID 12466265)

proof_lines: with N-methylglycine as methyl group acceptor, pH 8.8, 37°C

proof_lines: with glycine as methyl group acceptor, pH 8.8, 37°C

enzyme: glycine/sarcosine N-methyltransferase

substrate: N-methylglycine


organism: Halorhodospira halochloris

substrate_name: S-adenosyl-L-methionine

sequence_source: UniProtKB:Q9KJ22 (PMID 11319079)

proof_lines: recombinant enzyme, pH 7.4, 37°C, with sarcosine

proof_lines: recombinant enzyme, pH 7.4, 37°C, with S-adenosyl-L-methionine

proof_lines: recombinant enzyme, pH 7.4, 37°C, with glycine

substrate: S-adenosyl-L-methionine


organism: Galdieria sulphuraria

substrate_name: N,N-dimethylglycine

sequence_source: RCSB PDB:2O57 entity 1

proof_lines:
cosubstrate: N,N-dimethylglycine
cosubstrate: sarcosine

substrate: N,N-dimethylglycine


sequence_source: UniProtKB:Q9KJ21 (PMID 11319079)


organism: Methanohalophilus portucalensis

substrate_name: sarcosine

proof_lines: cosubstrate N,N-dimethylglycine, pH 7.2, 37°C

substrate: sarcosine


enzyme_display_name: flavonoid 4'-O-methyltransferase

organism: Camptotheca acuminata

enzyme: flavonoid 4'-O-methyltransferase


organism: Dianthus caryophyllus

substrate_name: apigenin

substrate: apigenin


organism: Mus musculus

substrate_name: histone H3 (T11phos)

substrate: histone H3 (T11phos)


organism: Bellis perennis

substrate_name: cyanidin 3-O-beta-glucoside

substrate: cyanidin 3-O-beta-glucoside


substrate_name: UDP-beta-D-glucuronate

substrate: UDP-beta-D-glucuronate


organism: Entada phaseoloides

substrate_name: luteolin

substrate: luteolin


organism: Panax ginseng

substrate_name: chikusetsusaponin IVa

substrate: chikusetsusaponin IVa


organism: Salmonella enterica

substrate_name: MECAM

proof_lines:
pH 9.0, 5mM MgCl2, 22°C
pH9.0, 5mM MgCl2, 22°C
pH 9.0, 5mM MgCl2, 22 °C

substrate: MECAM


organism: Bos taurus

substrate_name: CMP-N-glycolylneuraminate

proof_lines: ovine, CMP-N-acetylneuraminate as donor, 37°C

substrate: CMP-N-glycolylneuraminate


enzyme_display_name: phosphonoacetaldehyde hydrolase

organism: Achromobacter aegrifaciens

substrate_name: phosphonoacetaldehyde

enzyme: phosphonoacetaldehyde hydrolase

substrate: phosphonoacetaldehyde


organism: Achromobacter insolitus

proof_lines:
at pH 7.5 and 25°C
isoform PhnX19-II, at pH 7.5 and 25°C
isoform PhnX19-I, at pH 7.5 and 25°C


organism: Bacillus cereus


enzyme_display_name: galactolipase

organism: Oryza sativa

substrate_name: monogalactosyldiacylglycerol

enzyme: galactolipase

substrate: monogalactosyldiacylglycerol


organism: Phaseolus coccineus

substrate_name: digalactosyldiacylglycerol

substrate: digalactosyldiacylglycerol


organism: Phaseolus vulgaris

substrate_name: phosphatidylcholine

substrate: phosphatidylcholine


organism: Solanum tuberosum


organism: Vigna unguiculata


organism: Homo sapiens

proof_lines: pH and temperature not specified in the publication


organism: Ovis aries

substrate_name: submaxillary asialo-mucin

proof_lines: ovine, calculated in terms of N-acetylgalactosamine acceptor sites, 37°C

substrate: submaxillary asialo-mucin


organism: Rattus norvegicus

substrate_name: CMP-N-acetylneuraminate

substrate: CMP-N-acetylneuraminate


organism: Sus scrofa

proof_lines: pH 6.5, 37°C, ovine submaxillary asialo-mucin as acceptor

proof_lines: pH 6.5, 37°C, antifreeze glycoprotein 8 as acceptor


organism: Escherichia coli


organism: Gallus gallus

substrate_name: Ganglioside LM1

substrate: Ganglioside LM1


organism: Neisseria meningitidis

proof_lines: calculated and determined at constant donor concentration of 1 mM CMPNeu5Ac


substrate_name: ganglioside GM3

substrate: ganglioside GM3


enzyme_display_name: N-acylneuraminate-9-phosphate synthase

substrate_name: N-acetyl-D-mannosamine 6-phosphate

enzyme: N-acylneuraminate-9-phosphate synthase

substrate: N-acetyl-D-mannosamine 6-phosphate


enzyme_display_name: acetylesterase

organism: Orpinomyces sp.

substrate_name: p-nitrophenyl acetate

enzyme: acetylesterase

substrate: p-nitrophenyl acetate


organism: Pseudomonas putida

substrate_name: 4-nitrophenyl acetate

substrate: 4-nitrophenyl acetate


organism: Rhodococcus sp. (in: high G+C Gram-positive bacteria)

substrate_name: phenyl acetate

proof_lines:
recombinant enzyme, pH 6.4
recombinant enzyme, pH 8.0

substrate: phenyl acetate


organism: Rhodotorula mucilaginosa

substrate_name: acetylxylan

substrate: acetylxylan


organism: Streptomyces sp.


organism: Talaromyces pinophilus


substrate_name: 4-nitrophenyl butyrate

substrate: 4-nitrophenyl butyrate


organism: Thermotoga maritima

proof_lines: in 50 mM citrate-phosphate (pH 6) at 70°C


organism: Trypanosoma brucei

substrate_name: methylumbelliferone acetate

substrate: methylumbelliferone acetate


assay_conditions_summary: pH 7.1; 298.15 K; buffer 20 mM phosphate

proof_lines: pH 7.1, 25°C, recombinant wild-type enzyme

proof_lines: pH 7.1, 65°C, recombinant wild-type enzyme

proof_lines: wild type enzyme, in 20 mM phosphate buffer, at pH 7.1 and 30°C


assay_conditions_summary: pH 7.1; 303.15 K; buffer phosphate

proof_lines: mutant enzyme L97F, in 20 mM phosphate buffer, at pH 7.1 and 30°C


proof_lines: mutant enzyme L97F/L209F, in 20 mM phosphate buffer, at pH 7.1 and 30°C


proof_lines: mutant enzyme L209F, in 20 mM phosphate buffer, at pH 7.1 and 30°C


organism: Exiguobacterium antarcticum

substrate_name: 4-nitrophenyl hexanoate

substrate: 4-nitrophenyl hexanoate


organism: Fibrobacter succinogenes

substrate_name: Alpha-naphthyl acetate

substrate: Alpha-naphthyl acetate


organism: Kluyveromyces marxianus

substrate_name: (R)-1,2-O-isopropylidene glycol acetate

substrate: (R)-1,2-O-isopropylidene glycol acetate


organism: Lactiplantibacillus plantarum

substrate_name: Triacetin

substrate: Triacetin


organism: Lichtheimia corymbifera


organism: Meyerozyma guilliermondii


organism: Nocardia sp.

substrate_name: ethyl acetate

substrate: ethyl acetate


enzyme_display_name: acylcarnitine hydrolase

organism: Mus musculus

enzyme: acylcarnitine hydrolase


organism: Rattus norvegicus

substrate_name: H2O; L-Octanoylcarnitine

proof_lines:
low substrate concentration
high substrate concentration

substrate: H2O; L-Octanoylcarnitine


enzyme_display_name: sialate O-acetylesterase

organism: Bos taurus

substrate_name: 4-yethylumbelliferyl acetate

enzyme: sialate O-acetylesterase

substrate: 4-yethylumbelliferyl acetate


organism: Equus caballus

substrate_name: N-acetyl-9-O-acetylneuraminic acid

substrate: N-acetyl-9-O-acetylneuraminic acid


organism: Equus sp.

substrate_name: 4-O-acetyl-sialic acid

proof_lines: low pI esterase form, obtained with free sialic acid

proof_lines: high pI esterase form, obtained with free sialic acid

substrate: 4-O-acetyl-sialic acid


organism: influenza C virus

substrate_name: N-acetyl-9-O-lactoylneuraminic acid

substrate: N-acetyl-9-O-lactoylneuraminic acid


assay_conditions_summary: pH 7.5; 310.15 K; cofactor CoA


organism: Acinetobacter sp.

proof_lines: pH 7.0, 25°C, presence of benzethonium chloride

proof_lines: pH 7.0, 25°C, absence of benzethonium chloride


organism: Aspergillus niger


organism: Aspergillus oryzae


organism: Bacillus pumilus

substrate_name: 1-naphthol acetate

substrate: 1-naphthol acetate


substrate_name: H2O; p-Nitrophenyl acetate

substrate: H2O; p-Nitrophenyl acetate


enzyme_display_name: phosphomevalonate kinase

substrate_name: Unknown

enzyme: phosphomevalonate kinase

substrate: Unknown


organism: Streptococcus pneumoniae

substrate_name: ATP

assay_conditions_summary: pH 7; 298.15 K; cofactor ATP

substrate: ATP


organism: Sus scrofa

substrate_name: phosphomevalonate

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

proof_lines:
pH 7.5, 30°C
ATP in form of MgATP2-

substrate: phosphomevalonate


organism: Escherichia coli

substrate_name: adenylyl-[glutamine synthase]

assay_conditions_summary: pH 7.6; 303.15 K; cofactor ATP

proof_lines: adenylyl-removing activity, 0.00002 mM ATase, 1 mM alpha-ketoglutarate, 0.0005 mM signal transduction protein PII-UMP, 1 mM ATP, 5 mM potassium phosphate

proof_lines: adenylation of Mycobacterium tuberculosis [L-glutamate:ammonia ligase (ADP-forming)] expressed in Escherichia coli

substrate: adenylyl-[glutamine synthase]


substrate_name: dATP

assay_conditions_summary: pH 9; 298.15 K; cofactor ATP

proof_lines:
pH 9.0, 25°C
dATP in form of MgdATP2-
dGTP in form of MgdGTP2-

substrate: dATP


enzyme_display_name: streptomycin 3''-adenylyltransferase

organism: Bacillus subtilis

substrate_name: streptomycin

enzyme: streptomycin 3''-adenylyltransferase

substrate: streptomycin


organism: Serratia marcescens

proof_lines: kinetic parameter of the ANT(3)-Ii domain of the enzyme

proof_lines: Kii = 0.01 mM, dead-end inhibition of ANT(3)-Ii domain, substrate 0.1 mM ATP, uncompetitive inhibition


enzyme_display_name: amine sulfotransferase

organism: Cavia porcellus

proof_lines: with 2-naphthylamine as second substrate, pH 8, 37°C

enzyme: amine sulfotransferase


organism: Homo sapiens

proof_lines: biphasic kinetics, Km: 0.00013 mM and 0.0022 mM


organism: Mus musculus


organism: Rattus norvegicus


substrate_name: Phosphoenolpyruvate; H2O; N-Acetyl-D-mannosamine 6-phosphate


substrate_name: N-acetyl-D-mannosamine 6-phosphate

substrate: N-acetyl-D-mannosamine 6-phosphate


organism: Anaerocolumna aminovalerica

substrate_name: delta-aminovaleric acid

substrate: delta-aminovaleric acid


organism: Meyerozyma guilliermondii

proof_lines: pH 7.8, 35°C, noncompetitive inhibition with respect to delta-aminovalerate

proof_lines: pH 7.8, 35°C, competitive inhibition with respect to 2-oxoglutarate


organism: Pseudomonas sp.


enzyme_display_name: protein-fructosamine 3-kinase

enzyme: protein-fructosamine 3-kinase


enzyme_display_name: fatty acid kinase

organism: Spirochaetales

substrate_name: valerate

proof_lines: with isobutyrate as cosubstrate, at pH 7.5 and 30°C

enzyme: fatty acid kinase

substrate: valerate


assay_conditions_summary: pH 7; 303.15 K; cofactor ATP


proof_lines:
mutant R84M, 30°C, pH 7.0
mutant R84M, 30°C, pH 8.0


proof_lines:
mutant R111M, 30°C, pH 7.0
mutant R111M, 30°C, pH 8.0


enzyme_display_name: inosine nucleosidase

organism: Azotobacter vinelandii

substrate_name: Xanthosine

enzyme: inosine nucleosidase

substrate: Xanthosine


organism: Helianthus tuberosus

substrate_name: Inosine

substrate: Inosine


organism: Lupinus luteus


organism: Saccharolobus solfataricus

substrate_name: adenosine

substrate: adenosine