assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADH


enzyme_display_name: pyrroline-5-carboxylate reductase

organism: Zymomonas mobilis subsp. mobilis

source_db: brenda

verification_status: unverified

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADPH

proof_lines:
cosubstrate NADH, pH 7.5, 30°C
cosubstrate NADPH, pH 7.5, 30°C

enzyme: pyrroline-5-carboxylate reductase

status: unverified

source: brenda


organism: Escherichia coli

substrate_name: methylenetetrahydrofolate

proof_lines: recombinant wild-type enzyme, pH 7.2, 4°C

substrate: methylenetetrahydrofolate


substrate_name: NADH

assay_conditions_summary: pH 7.2; 298.15 K; cofactor NADH

proof_lines: cosubstrate 5,10-methylenetetrahydrofolate, wild-type, pH 7.2, 25°C

proof_lines: cosubstrate menadione, wild-type, pH 7.2, 25°C

proof_lines: recombinant wild-type enzyme, pH 7.2, 4°C, the mutant enzymes all show a Km below 0.0035 mM

substrate: NADH


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution

proof_lines: cosubstrate 5,10-methylenetetrahydrofolate, mutant F223L, pH 7.2, 25°C

proof_lines: cosubstrate menadione, mutant F223L, pH 7.2, 25°C


proof_lines: cosubstrate 5,10-methylenetetrahydrofolate, mutant F223A, pH 7.2, 25°C

proof_lines: cosubstrate menadione, mutant F223A, pH 7.2, 25°C


proof_lines:
recombinant mutant D120N, pH 7.2, 4°C
D120N mutant enzyme


assay_conditions_summary: cofactor NADH


organism: Bos taurus

mutation_signature: Δ1

mutation_type: deletion

proof_lines: reaction with DELTA1-piperidine 2-carboxylate


substrate_name: S-Aminoethylcysteine ketimine

smiles: CC(N)SCC(C(=O)N)N

substrate: S-Aminoethylcysteine ketimine


substrate_name: DELTA1-Piperidine 2-carboxylate

substrate: DELTA1-Piperidine 2-carboxylate


substrate_name: Lanthionine ketimine

assay_conditions_summary: cofactor NADPH

substrate: Lanthionine ketimine


substrate_name: Cystathionine ketimine

substrate: Cystathionine ketimine


organism: Rattus norvegicus

substrate_name: Unknown

km_display: Not reported

ki_unit: mM

assay_conditions_summary: pH 5

proof_lines: with substrate S-(2-aminoethyl)-L-cysteine ketimine, pH 5.0, temperature not specified in the publication

substrate: Unknown


enzyme_display_name: dihydrofolate reductase

organism: Cricetulus griseus

enzyme: dihydrofolate reductase


substrate_name: NADPH

substrate: NADPH


organism: Photobacterium profundum

assay_conditions_summary: pH 7; 298.15 K; cofactor NADPH

proof_lines: pH 7.0, 25°C, under atmospheric pressure


substrate_name: dihydrofolate

substrate: dihydrofolate


organism: Trypanosoma brucei

proof_lines: wild type enzyme, in 50 mM TES, pH 7.0, at 37°C


proof_lines: mutant enzyme T86N, in 50 mM TES, pH 7.0, at 37°C


proof_lines: mutant enzyme T86S, in 50 mM TES, pH 7.0, at 37°C


assay_conditions_summary: pH 7; 310.15 K; cofactor NADPH


proof_lines:
pH 7.4, 25°C, recombinant enzyme
recombinant bifunctional enzyme


organism: Bacillus sp. (in: firmicutes)

substrate_name: N-Methyl-DL-alanine

smiles: CC(C(=O)O)NC

substrate: N-Methyl-DL-alanine


substrate_name: N-Methyl-L-leucine

smiles: CC(C)CC(C(=O)O)NC

substrate: N-Methyl-L-leucine


substrate_name: N-Methyl-DL-valine

smiles: CC(C)C(C(=O)O)NC

substrate: N-Methyl-DL-valine


substrate_name: sarcosine

smiles: CNCC(=O)O

proof_lines: native enzyme, reconstructed with 20% dimethyl sulfoxide, pH 8.0, 37°C

proof_lines: native enzyme, reconstructed in aqueous, pH 8.0, 37°C

substrate: sarcosine


organism: Streptomyces sp.


organism: Cereibacter sphaeroides


enzyme_display_name: hydroxyproline dehydrogenase

organism: Homo sapiens

enzyme: hydroxyproline dehydrogenase


substrate_name: coenzyme Q1

substrate: coenzyme Q1


substrate_name: duroquinone

substrate: duroquinone


substrate_name: menaquinone

substrate: menaquinone


substrate_name: menadione

substrate: menadione


substrate_name: L-proline

substrate: L-proline


enzyme_display_name: cytochrome-b5 reductase

organism: Musca domestica

enzyme: cytochrome-b5 reductase


substrate_name: ferricyanide

substrate: ferricyanide


substrate_name: NADH

substrate: NADH


substrate_name: ferricytochrome b5

substrate: ferricytochrome b5


enzyme_display_name: NADPH--hemoprotein reductase

organism: Andrographis paniculata

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADPH

enzyme: NADPH--hemoprotein reductase


substrate_name: ferricytochrome c

substrate: ferricytochrome c


organism: Artemisia annua

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADH


assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH


organism: Camptotheca acuminata


organism: Candida tropicalis

proof_lines:
grown on alkanes
grown on glycerol


enzyme_display_name: demethylphylloquinone reductase

organism: Arabidopsis thaliana

enzyme: demethylphylloquinone reductase


enzyme_display_name: GMP reductase

organism: Mycoplasma mycoides

substrate_name: GMP

enzyme: GMP reductase

substrate: GMP


enzyme_display_name: nitrite reductase (NO-forming)

organism: Geobacillus kaustophilus

substrate_name: ferricytochrome c551

enzyme: nitrite reductase (NO-forming)

substrate: ferricytochrome c551


organism: Haloferax mediterranei

substrate_name: reduced methyl viologen

proof_lines: pH and temperature not specified in the publication

substrate: reduced methyl viologen


substrate_name: nitrite

substrate: nitrite


organism: Nicotiana tabacum

substrate_name: N-acetyl-S-geranylgeranyl-L-cysteine

substrate: N-acetyl-S-geranylgeranyl-L-cysteine


substrate_name: N-acetyl-S-(E,E)-farnesyl-L-cysteine

substrate: N-acetyl-S-(E,E)-farnesyl-L-cysteine


proof_lines:
recombinant enzyme
native enzyme


organism: Candida albicans

proof_lines: in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C

proof_lines: 37°C, pH not specified in the publication


substrate_name: L-homocysteine

smiles: C(CS)C(C(=O)O)N

substrate: L-homocysteine


enzyme_display_name: (iso)eugenol O-methyltransferase

organism: Ocimum basilicum

substrate_name: chavicol

enzyme: (iso)eugenol O-methyltransferase

substrate: chavicol


substrate_name: t-isoeugenol

substrate: t-isoeugenol


substrate_name: eugenol

substrate: eugenol


substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


substrate_name: trans-isoeugenol

substrate: trans-isoeugenol


enzyme_display_name: glycine N-methyltransferase

enzyme: glycine N-methyltransferase


substrate_name: Gly

smiles: C(C(=O)O)N

substrate: Gly


organism: Rattus norvegicus

substrate_name: S-adenosylmethionine

substrate: S-adenosylmethionine


substrate_name: glycine

substrate: glycine


proof_lines:
pH 7.5, 25°C, native enzyme
pH 7.5, 25°C, recombinant enzyme


substrate_name: cytosine38 in tRNAGly(GHCC)

substrate: cytosine38 in tRNAGly(GHCC)


organism: Medicago truncatula


organism: Aspergillus fumigatus


substrate_name: 6-hydroxytryprostatin B

substrate: 6-hydroxytryprostatin B


organism: Methanothermobacter thermautotrophicus

substrate_name: formylmethanofuran

substrate: formylmethanofuran


substrate_name: N-Furfurylformamide

substrate: N-Furfurylformamide


enzyme_display_name: lovastatin nonaketide synthase

organism: Aspergillus terreus

proof_lines: pH not specified in the publication, temperature not specified in the publication

enzyme: lovastatin nonaketide synthase


enzyme_display_name: 6-methylsalicylic acid synthase

proof_lines: release from enzyme/acyl-carrier-protein, recombinant His6-tagged THID mutant, pH 7.5, 25°C

enzyme: 6-methylsalicylic acid synthase


proof_lines: release from enzyme/acyl-carrier-protein, recombinant His6-tagged ATX, pH 7.5, 25°C


enzyme_display_name: fatty-acid synthase system

organism: Gallus gallus

substrate_name: acetyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: fatty-acid synthase system

substrate: acetyl-CoA


substrate_name: malonyl-CoA

substrate: malonyl-CoA


substrate_name: Unknown

proof_lines:
substrate NADPH, pH 7.0, 37°C
substrate acetyl-CoA, pH 7.0, 37°C
substrate NADPH

substrate: Unknown


substrate_name: NADPH

substrate: NADPH


substrate_name: myristoyl-CoA

substrate: myristoyl-CoA


substrate_name: Gly-Asn-Ala-Ala-Ser-Ala-Arg-Arg

substrate: Gly-Asn-Ala-Ala-Ser-Ala-Arg-Arg


substrate_name: Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg

substrate: Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg


organism: Leishmania donovani

assay_conditions_summary: pH 7.4; 310.15 K; cofactor CoA


substrate_name: ARF peptide

substrate: ARF peptide


enzyme_display_name: citrate (Si)-synthase

organism: Homo sapiens

substrate_name: oxaloacetate

assay_conditions_summary: pH 8

proof_lines: wild-type, pH 8.0, temperature not specified in the publication

proof_lines: pH 8.0, temperature not specified in the publication, wild-type enzyme

enzyme: citrate (Si)-synthase

substrate: oxaloacetate


assay_conditions_summary: pH 8; cofactor CoA


proof_lines: substrate acetyl-CoA, pH 8.0, temperature not specified in the publication

proof_lines: pH 8.0, temperature not specified in the publication, substrate: propionyl-CoA

proof_lines: substrate oxaloacetate, pH 8.0, temperature not specified in the publication


enzyme_display_name: hydroxymethylglutaryl-CoA synthase

organism: Brassica juncea

substrate_name: acetoacetyl-CoA

assay_conditions_summary: pH 7.5; 303.15 K; cofactor CoA

enzyme: hydroxymethylglutaryl-CoA synthase

substrate: acetoacetyl-CoA


enzyme_display_name: ATP citrate synthase

organism: Chlorobium limicola

substrate_name: ATP

assay_conditions_summary: cofactor ATP

enzyme: ATP citrate synthase

substrate: ATP


substrate_name: CoA

substrate: CoA


substrate_name: citrate

substrate: citrate


substrate_name: UDP-galactose

substrate: UDP-galactose


substrate_name: A-P-(N-acetyl-D-galactosaminyl)T-S-S-A

substrate: A-P-(N-acetyl-D-galactosaminyl)T-S-S-A


enzyme_display_name: isoflavone 7-O-glucosyltransferase

organism: Pueraria montana var. lobata

substrate_name: daidzein

enzyme: isoflavone 7-O-glucosyltransferase

substrate: daidzein


substrate_name: genistein

substrate: genistein


organism: Staphylococcus aureus

substrate_name: UDP-N-acetyl-D-glucosamine

proof_lines: recombinant full-length protein, pH not specified in the publication, temperature not specified in the publication

substrate: UDP-N-acetyl-D-glucosamine


mutation_signature: truncated protein

mutation_type: truncation

proof_lines: truncated protein, residues 1-349, pH not specified in the publication, temperature not specified in the publication


substrate_name: polyribitol phosphate

substrate: polyribitol phosphate


organism: Biomphalaria glabrata

substrate_name: UDP-N-acetyl-alpha-D-galactosamine

proof_lines: with polypeptide Muc2 as cosubstrate, at pH 6.0 and 37°C

substrate: UDP-N-acetyl-alpha-D-galactosamine


substrate_name: polypeptide Muc2

substrate: polypeptide Muc2


organism: Cryptosporidium parvum


enzyme_display_name: monogalactosyldiacylglycerol synthase

organism: Spinacia oleracea

substrate_name: UDPgalactose

enzyme: monogalactosyldiacylglycerol synthase

substrate: UDPgalactose


substrate_name: diacylglycerol

substrate: diacylglycerol


substrate_name: Unknown

proof_lines: dioleoylglycerol as the varied substrate

substrate: Unknown


substrate_name: dioleoylglycerol

proof_lines: in envelope membrane vesicles, at 0.25 mg/ml protein

substrate: dioleoylglycerol


substrate_name: GDP-L-fucose

substrate: GDP-L-fucose


proof_lines: pH and temperature not specified in the publication


organism: Mus musculus

substrate_name: GDP-L-Fuc

substrate: GDP-L-Fuc


enzyme_display_name: type 1 galactoside alpha-(1,2)-fucosyltransferase

organism: Helicobacter pylori

substrate_name: beta-lactose

proof_lines:
pH 7.5, 37°C, wild-type enzyme
pH 5.0, 37°C, wild-type enzyme

substrate: beta-lactose


substrate_name: GDP-beta-L-fucose

enzyme: type 1 galactoside alpha-(1,2)-fucosyltransferase

substrate: GDP-beta-L-fucose


organism: Candida albicans

substrate_name: L-gamma-glutamyl-p-nitroanilide

proof_lines: wild-type with N-terminal His-tag, pH 7.5, 25°C

proof_lines: wild-type with C-terminal His-tag, pH 7.5, 25°C

substrate: L-gamma-glutamyl-p-nitroanilide


substrate_name: D-fructose 6-phosphate

assay_conditions_summary: pH 7.5; 298.15 K; buffer in 25 mM potassium phosphate

proof_lines: hexose phosphate-isomerizing activity, wild-type with C-terminal His-tag, pH 7.5, 25°C

proof_lines: hexose phosphate-isomerizing activity, wild-type, pH 7.5, 25°C

proof_lines: hexose phosphate-isomerizing activity, wild-type with N-terminal His-tag, pH 7.5, 25°C

substrate: D-fructose 6-phosphate


assay_conditions_summary: pH 7.5; 298.15 K; buffer 20 mM HEPES

proof_lines: residues 1-345, His-tagged glutamine amide-hydrolysing domain, pH 7.5, 25°C

proof_lines: reaction mixture contains 1 mM L-gamma-glutamyl-p-nitroanilide, 1 mM EDTA, 1 mM DTT and the appropriately diluted enzyme preparation in 20 mM HEPES buffer (pH 7.5) at 25°C


assay_conditions_summary: pH 7.5; 298.15 K; buffer 50 mM Tris/HCl; cofactor NADP+

proof_lines: hexose phosphate-isomerizing activity, residues 346-712, His-tagged hexose phosphate-isomerizing domain, pH 7.5, 25°C

proof_lines: reaction mixture contains 2 mM D-fructose 6-phosphate, 0.5 mM NADP+, 1 mM EDTA, and appropriately diluted GlcN-6-P synthase preparation in 50 mM Tris/HCl buffer (pH 7.5) at 25°C


substrate_name: L-glutamate

smiles: C(CC(=O)O)C(C(=O)O)N

proof_lines: reaction mixture contains 10 mM L-glutamate, 1 mM EDTA, 1 mM DTT and an appropriately diluted enzyme preparation in 20 mM HEPES buffer (pH 7.5) at 25°C

substrate: L-glutamate


substrate_name: L-glutamine

smiles: C(CC(=O)N)C(C(=O)O)N

proof_lines: glutamine 6-phosphate-synthetic activity, wild-type, pH 7.5, 25°C

substrate: L-glutamine


substrate_name: L-gamma -glutamyl-p-nitroanilide

substrate: L-gamma -glutamyl-p-nitroanilide


proof_lines: mutant mutant Gfa1-His6DELTA655-660, pH 6.8, 37°C


proof_lines: mutant Gfa1-His6DELTA343-348, pH 6.8, 37°C


proof_lines: mutant mutant Gfa1-K568H/S569H, pH 6.8, 37°C


proof_lines: mutant Gfa1-His6DELTA655-660, pH 6.8, 37°C


enzyme_display_name: hexokinase

organism: Botrytis cinerea

substrate_name: D-glucose

enzyme: hexokinase

substrate: D-glucose


substrate_name: D-fructose

substrate: D-fructose


organism: Sporolactobacillus inulinus

substrate_name: ATP

assay_conditions_summary: pH 8.5; 303.15 K; cofactor ATP

substrate: ATP


enzyme_display_name: gluconokinase

organism: Zymomonas mobilis

substrate_name: D-gluconate

enzyme: gluconokinase

substrate: D-gluconate


enzyme_display_name: glucokinase

organism: Geobacillus stearothermophilus

substrate_name: alpha-D-glucose

enzyme: glucokinase

substrate: alpha-D-glucose


substrate_name: GTP

substrate: GTP


substrate_name: ITP

substrate: ITP


substrate_name: CTP

substrate: CTP


substrate_name: UTP

substrate: UTP


assay_conditions_summary: pH 7; 303.15 K; cofactor ATP

proof_lines:
30°C, pH 7
+ CTP, 30°C, pH 7
+ UTP, 30°C, pH 7


proof_lines:
30°C, pH 7
ATP in form of MgATP2-


enzyme_display_name: fructokinase

enzyme: fructokinase


assay_conditions_summary: pH 7.4; 303.15 K; cofactor ATP

proof_lines:
with ATP as cosubstrate, pH 7.4, 30°C
with UTP as cosubstrate, pH 7.4, 30°C
with ITP as cosubstrate, pH 7.4, 30°C


enzyme_display_name: pyruvate, phosphate dikinase

organism: Trypanosoma brucei

substrate_name: AMP

enzyme: pyruvate, phosphate dikinase

substrate: AMP


substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


substrate_name: diphosphate

substrate: diphosphate


substrate_name: pyruvate

substrate: pyruvate


substrate_name: phosphate

substrate: phosphate


enzyme_display_name: alpha-glucan, water dikinase

organism: Solanum tuberosum

enzyme: alpha-glucan, water dikinase


substrate_name: granular potato starch

substrate: granular potato starch


organism: Thauera aromatica


substrate_name: succinate

substrate: succinate


enzyme_display_name: poly(ethylene terephthalate) hydrolase

organism: Thermomonospora curvata

substrate_name: 4-nitrophenyl butanoate

enzyme: poly(ethylene terephthalate) hydrolase

substrate: 4-nitrophenyl butanoate


organism: Podospora anserina

proof_lines:
pH 6.0, 50°C
pH 7.0, 50°C, recombinant enzyme
pH 6.0, 35°C


enzyme_display_name: feruloyl esterase

organism: Thermothelomyces thermophilus

substrate_name: n-hexyl ferulate

enzyme: feruloyl esterase

substrate: n-hexyl ferulate


substrate_name: ethyl ferulate

substrate: ethyl ferulate


substrate_name: n-propyl ferulate

substrate: n-propyl ferulate


substrate_name: methyl caffeate

substrate: methyl caffeate


substrate_name: methyl sinapate

substrate: methyl sinapate


substrate_name: methyl 4-coumarate

substrate: methyl 4-coumarate


substrate_name: methyl ferulate

substrate: methyl ferulate


enzyme_display_name: cutinase

organism: Aspergillus nidulans

substrate_name: 4-nitrophenyl acetate

enzyme: cutinase

substrate: 4-nitrophenyl acetate


organism: Enterobacter sp. CS66

substrate_name: N-butanoyl-L-homoserine lactone

substrate: N-butanoyl-L-homoserine lactone


substrate_name: N-hexanoyl-L-homoserine lactone

substrate: N-hexanoyl-L-homoserine lactone


substrate_name: N-octanoyl-L-homoserine lactone

substrate: N-octanoyl-L-homoserine lactone


substrate_name: N-decanoyl-L-homoserine lactone

substrate: N-decanoyl-L-homoserine lactone


substrate_name: N-3-oxododecanoyl-L-homoserine lactone

substrate: N-3-oxododecanoyl-L-homoserine lactone


enzyme_display_name: acyl-CoA hydrolase

organism: Streptomyces chartreusis

substrate_name: palmitoyl-CoA

enzyme: acyl-CoA hydrolase

substrate: palmitoyl-CoA


substrate_name: benzoyl-CoA

substrate: benzoyl-CoA


substrate_name: malonyl-CoA

substrate: malonyl-CoA


substrate_name: acetyl-CoA

substrate: acetyl-CoA


substrate_name: butyryl-CoA

substrate: butyryl-CoA


organism: Hordeum vulgare

substrate_name: Inositol hexakisphosphate

substrate: Inositol hexakisphosphate


organism: Rattus norvegicus

proof_lines:
pH 7.4, 37°C
pH 7.2, 37°C, cytosolic enzyme


substrate_name: Inositol 1,3,4,5,6-pentakisphosphate

substrate: Inositol 1,3,4,5,6-pentakisphosphate


enzyme_display_name: glycerophosphodiester phosphodiesterase

organism: Bacillus altitudinis

substrate_name: bis(4-nitrophenyl) phosphate

proof_lines: pH and temperature not specified in the publication

enzyme: glycerophosphodiester phosphodiesterase

substrate: bis(4-nitrophenyl) phosphate


enzyme_display_name: cellulose 1,4-beta-cellobiosidase (non-reducing end)

organism: Rasamsonia emersonii


substrate_name: cellulose

enzyme: cellulose 1,4-beta-cellobiosidase (non-reducing end)

substrate: cellulose


substrate_name: 4-nitrophenyl beta-D-lactoside

substrate: 4-nitrophenyl beta-D-lactoside


enzyme_display_name: endo-alpha-N-acetylgalactosaminidase

organism: Clostridium perfringens

enzyme: endo-alpha-N-acetylgalactosaminidase


organism: [Clostridium] nexile

proof_lines: above 5 mM, pH 6.0, 37°C, O-glycanases Tn2105


enzyme_display_name: arabinan endo-1,5-alpha-L-arabinosidase

organism: Aspergillus aculeatus

enzyme: arabinan endo-1,5-alpha-L-arabinosidase


enzyme_display_name: leukotriene-A4 hydrolase

substrate_name: leukotriene A4

enzyme: leukotriene-A4 hydrolase

substrate: leukotriene A4


enzyme_display_name: microsomal epoxide hydrolase

organism: Mus musculus

substrate_name: trans-beta-ethylstyrene oxide

enzyme: microsomal epoxide hydrolase

substrate: trans-beta-ethylstyrene oxide


substrate_name: trans-stilbene oxide

substrate: trans-stilbene oxide


enzyme_display_name: aspartyl aminopeptidase

organism: Schistosoma japonicum

proof_lines: 37°C, pH not specified in the publication

enzyme: aspartyl aminopeptidase


enzyme_display_name: tripeptidyl-peptidase II

substrate_name: Unknown

enzyme: tripeptidyl-peptidase II

substrate: Unknown


substrate_name: Arg-Arg-Ala-(phospho)Ser-Val-Ala

substrate: Arg-Arg-Ala-(phospho)Ser-Val-Ala


enzyme_display_name: Xaa-Pro dipeptidyl-peptidase

organism: Lactococcus cremoris

proof_lines: 37°C, pH 7.5, mutant enzyme V471I, calculated by BRENDA

enzyme: Xaa-Pro dipeptidyl-peptidase


proof_lines: 37°C, pH 7.5, mutant enzyme I374L, calculated by BRENDA


proof_lines: 37°C, pH 7.5, wild-type enzyme, calculated by BRENDA


organism: Latilactobacillus sakei


enzyme_display_name: dipeptidyl-peptidase IV

organism: Cavia porcellus

enzyme: dipeptidyl-peptidase IV


enzyme_display_name: t-plasminogen activator

enzyme: t-plasminogen activator


enzyme_display_name: pancreatic elastase II

enzyme: pancreatic elastase II


substrate_name: Suc-Ala-Ala-Pro-Met-p-nitroanilide

substrate: Suc-Ala-Ala-Pro-Met-p-nitroanilide


enzyme_display_name: cathepsin B

organism: Capra hircus

substrate_name: hemoglobin

proof_lines: depending on concentration of dimethylsulfoxide, 1-2.5%

enzyme: cathepsin B

substrate: hemoglobin


proof_lines:
pH 6.0, 37°C, recombinant enzyme
liver enzyme
tumor enzyme


substrate_name: benzoyl-Pro-Phe-Arg-p-nitroanilide

proof_lines:
liver enzyme
tumor enzyme

substrate: benzoyl-Pro-Phe-Arg-p-nitroanilide


enzyme_display_name: renin

organism: Canis lupus familiaris

enzyme: renin


enzyme_display_name: microbial collagenase

organism: Clostridium tetani

substrate_name: furylacryloyl-Leu-Gly-Pro-Ala

proof_lines: isoform ColT with N-terminal His6-tag, pH 7.5, 25°C

enzyme: microbial collagenase

substrate: furylacryloyl-Leu-Gly-Pro-Ala


organism: Hathewaya histolytica

substrate_name: gelatin

substrate: gelatin


organism: Lysinibacillus sphaericus

substrate_name: Collagen

substrate: Collagen


enzyme_display_name: ADAMTS-4 endopeptidase

organism: Sus scrofa

proof_lines: pH 7.5, 37°C, substrate: pH 7.5, 37°C, substrate: Lys(7-methoxycoumarin-4-yl)-Lys-Gln-Glu-Phe-Arg-Gly-Gln-Thr-Lys(Dnp)-NH2

enzyme: ADAMTS-4 endopeptidase


enzyme_display_name: protein deglycase

organism: Homo sapiens

proof_lines: pH not specified in the publication, temperature not specified in the publication

enzyme: protein deglycase


substrate_name: N-acetyl-L-arginine

smiles: CC(=O)NC(CCCN=C(N)N)C(=O)O

substrate: N-acetyl-L-arginine


substrate_name: N-acetyl-L-lysine

smiles: CC(=O)NC(CCCCN)C(=O)O

substrate: N-acetyl-L-lysine


substrate_name: N-acetyl-L-cysteine

smiles: CC(=O)NC(CS)C(=O)O

substrate: N-acetyl-L-cysteine


substrate_name: methylglyoxal

smiles: CC(=O)C=O

substrate: methylglyoxal


substrate_name: 4-nitrophenyl acetate

substrate: 4-nitrophenyl acetate


enzyme_display_name: ADP-ribose diphosphatase

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ADP

enzyme: ADP-ribose diphosphatase


substrate_name: ADP-D-ribose

proof_lines:
at pH 7.5 and 37°C
pH 9.0, 37°C

substrate: ADP-D-ribose


substrate_name: ADP-mannose

assay_conditions_summary: pH 9; 310.15 K; cofactor ADP

substrate: ADP-mannose


enzyme_display_name: nucleoside-triphosphate phosphatase

organism: Rattus norvegicus

substrate_name: ATP

enzyme: nucleoside-triphosphate phosphatase

substrate: ATP


enzyme_display_name: inosine diphosphate phosphatase

substrate_name: XDP

enzyme: inosine diphosphate phosphatase

substrate: XDP


substrate_name: ITP

substrate: ITP


substrate_name: ADP

substrate: ADP


substrate_name: Unknown

proof_lines:
substrate IDP, pH 7.5, 37°C
substrate GDP, pH 7.5, 37°C

substrate: Unknown


substrate_name: dGDP

substrate: dGDP


substrate_name: GDP

substrate: GDP


substrate_name: IDP

substrate: IDP


substrate_name: dIDP

substrate: dIDP


substrate_name: dITP

substrate: dITP


enzyme_display_name: nucleotide diphosphatase

organism: Ovis aries aries

substrate_name: FAD

assay_conditions_summary: cofactor FAD

proof_lines: with and without phosphate which abolishes negative cooperative interaction

enzyme: nucleotide diphosphatase

substrate: FAD


organism: Arthrobacter sp. YC-RL1


enzyme_display_name: (S)-2-haloacid dehalogenase

organism: Pseudomonas aeruginosa

enzyme: (S)-2-haloacid dehalogenase


organism: Pseudomonas putida

substrate_name: Monochloroacetate

substrate: Monochloroacetate


enzyme_display_name: haloalkane dehalogenase

organism: Sphingobium indicum

proof_lines: pH 7.5, 30°C, recombinant wild-type enzyme

enzyme: haloalkane dehalogenase


substrate_name: beta-hexachlorocyclohexane

substrate: beta-hexachlorocyclohexane


smiles: C(C(CCl)Cl)Cl


enzyme_display_name: pyruvate decarboxylase

organism: Populus deltoides

substrate_name: pyruvate

assay_conditions_summary: pH 5.6; 298.15 K; buffer 100 mM MES

proof_lines: in 100 mM MES buffer (pH 5.6), 5 mM MgCl2, 1 mM dithiothreitol, and 1 mM thiamine diphosphate, at 25°C

enzyme: pyruvate decarboxylase

substrate: pyruvate


substrate_name: 2-ketobutyric acid

smiles: CCC(=O)C(=O)O

substrate: 2-ketobutyric acid


enzyme_display_name: oxalate decarboxylase

organism: Pseudomonas sp.

substrate_name: oxalate

enzyme: oxalate decarboxylase

substrate: oxalate


enzyme_display_name: acetoacetate decarboxylase

organism: Chromobacterium violaceum

substrate_name: acetoacetate

assay_conditions_summary: pH 5.95; 298.15 K; buffer 50 mM phosphate

proof_lines: at 25°C, in 50 mM phosphate buffer, pH 5.95

enzyme: acetoacetate decarboxylase

substrate: acetoacetate


enzyme_display_name: cis-aconitate decarboxylase

organism: Aspergillus terreus

substrate_name: cis-aconitate

enzyme: cis-aconitate decarboxylase

substrate: cis-aconitate


proof_lines: pH 6.5, 37°C, recombinant wild-type enzyme

proof_lines: wild-type, pH 6.5, 37°C, 167 mM phosphate buffer


enzyme_display_name: (S)-malate dehydrogenase (NAD(+), oxaloacetate-forming)

organism: Comamonas testosteroni

substrate_name: H+; Oxaloacetate; NADH

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

assay_conditions_summary: cofactor NADH

substrate: H+; Oxaloacetate; NADH


enzyme_display_name: isocitrate dehydrogenase (NAD(+))

organism: Teladorsagia circumcincta

substrate_name: NAD+; Isocitrate

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

assay_conditions_summary: cofactor NAD+

enzyme: isocitrate dehydrogenase (NAD(+))

substrate: NAD+; Isocitrate


enzyme_display_name: isocitrate dehydrogenase (NADP(+))

organism: Sulfolobus solfataricus

substrate_name: NADP+; Isocitrate

assay_conditions_summary: cofactor NADP+

enzyme: isocitrate dehydrogenase (NADP(+))

substrate: NADP+; Isocitrate


enzyme_display_name: glucose 1-dehydrogenase [NAD(P)(+)]

organism: Anas platyrhynchos

substrate_name: NAD+; beta-D-Glucose

enzyme: glucose 1-dehydrogenase [NAD(P)(+)]

substrate: NAD+; beta-D-Glucose


enzyme_display_name: malate dehydrogenase (NADP(+))

organism: Glycine max

substrate_name: Oxaloacetate; NADPH; H+

assay_conditions_summary: cofactor NADPH

enzyme: malate dehydrogenase (NADP(+))

substrate: Oxaloacetate; NADPH; H+


organism: Mycobacterium bovis

substrate_name: Pyruvate; Coenzyme A; NAD+

substrate: Pyruvate; Coenzyme A; NAD+


organism: Homo sapiens

substrate_name: NADP+; Acenaphthen-1-ol

substrate: NADP+; Acenaphthen-1-ol


enzyme_display_name: succinate dehydrogenase

organism: Neurospora crassa

substrate_name: Succinate; Acceptor

enzyme: succinate dehydrogenase

substrate: Succinate; Acceptor


organism: Plasmodium yoelii yoelii

substrate_name: Succinate; Ubiquinone-2

substrate: Succinate; Ubiquinone-2


organism: Nicotiana tabacum


organism: Mytilus galloprovincialis

substrate_name: ATP; D-Fructose 6-phosphate

substrate: ATP; D-Fructose 6-phosphate


organism: Saccharomyces pastorianus


organism: Saccharum officinarum

substrate_name: D-Fructose 6-phosphate; ATP

substrate: D-Fructose 6-phosphate; ATP


enzyme_display_name: glucokinase

organism: Geobacillus stearothermophilus

substrate_name: ATP; Glucose

enzyme: glucokinase

substrate: ATP; Glucose


organism: Scheffersomyces stipitis

substrate_name: Pyruvate

substrate: Pyruvate


organism: Lathyrus oleraceus

substrate_name: (S)-malate

substrate: (S)-malate


substrate_name: NADP+

substrate: NADP+


enzyme_display_name: glucose oxidase

organism: Talaromyces pinophilus

enzyme: glucose oxidase


substrate_name: beta-D-glucose

substrate: beta-D-glucose


enzyme_display_name: hydrogen dehydrogenase

organism: Cupriavidus necator

substrate_name: oxidized resazurin

enzyme: hydrogen dehydrogenase

substrate: oxidized resazurin


substrate_name: ferricyanide

substrate: ferricyanide


substrate_name: benzyl viologen

substrate: benzyl viologen


substrate_name: methyl viologen

substrate: methyl viologen


enzyme_display_name: catechol 1,2-dioxygenase

organism: Aspergillus niger

substrate_name: catechol

enzyme: catechol 1,2-dioxygenase

substrate: catechol


enzyme_display_name: gibberellin 3beta-dioxygenase

organism: Phaseolus vulgaris

substrate_name: gibberellin a20

enzyme: gibberellin 3beta-dioxygenase

substrate: gibberellin a20


substrate_name: gibberellin a9

substrate: gibberellin a9


enzyme_display_name: lovastatin nonaketide synthase

organism: Aspergillus terreus

substrate_name: malonyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: lovastatin nonaketide synthase

substrate: malonyl-CoA


enzyme_display_name: type 1 galactoside alpha-(1,2)-fucosyltransferase

organism: Canis lupus familiaris

substrate_name: lactose

substrate: lactose


organism: Zymomonas mobilis

assay_conditions_summary: cofactor ATP


enzyme_display_name: acyloxyacyl hydrolase

organism: Oryctolagus cuniculus

substrate_name: lipopolysaccharide

enzyme: acyloxyacyl hydrolase

substrate: lipopolysaccharide


organism: Brassica napus

substrate_name: acetoacetyl-[acyl-carrier protein]

substrate: acetoacetyl-[acyl-carrier protein]


substrate_name: NADPH

substrate: NADPH


smiles: CC(=O)NCCS


substrate_name: acetoacetyl-CoA

substrate: acetoacetyl-CoA


assay_conditions_summary: pH 8; 303.15 K; cofactor NADPH


assay_conditions_summary: pH 8; 303.15 K; cofactor CoA


organism: Escherichia coli

substrate_name: ethyl acetoacetate

smiles: CCOC(=O)CC(=O)C

substrate: ethyl acetoacetate


organism: Lactococcus lactis

substrate_name: beta-ketobutyryl-CoA

assay_conditions_summary: pH 7; 303.15 K; cofactor CoA

proof_lines:
pH 7.0, 30°C, FabG1
pH 7.0, 30°C, FabG2

substrate: beta-ketobutyryl-CoA


organism: Mycobacterium tuberculosis

assay_conditions_summary: pH 7; 298.15 K; cofactor CoA


assay_conditions_summary: pH 7.6; 303.15 K; cofactor NADPH


organism: Mycolicibacterium smegmatis


organism: Plasmodium falciparum


assay_conditions_summary: pH 7.5; 295.15 K; cofactor CoA

proof_lines:
pH 7.5
pH 6.8, 22°C, recombinant enzyme
pH 6.8, 25°C, recombinant enzyme


assay_conditions_summary: pH 7; 298.15 K; cofactor NADPH

proof_lines: in the presence of 4 M urea, pH 7.0, 25°C

proof_lines: in the presence of 1.5 M urea, pH 7.0, 25°C


substrate_name: Unknown

substrate: Unknown


organism: Pseudomonas aeruginosa

proof_lines: with acetoacetyl-CoA as cosubstrate, pH and temperature not specified in the publication


substrate_name: NADP+

proof_lines: with 3-hydroxydecanoyl-N-acetylcysteamine as cosubstrate, pH and temperature not specified in the publication

substrate: NADP+


proof_lines: pH and temperature not specified in the publication


organism: Saccharomyces cerevisiae

assay_conditions_summary: pH 8; 298.15 K; cofactor NADPH


assay_conditions_summary: pH 8; 298.15 K; cofactor CoA


organism: Streptococcus pneumoniae

assay_conditions_summary: pH 7.6; 303.15 K; cofactor CoA


enzyme_display_name: 3-dehydrosphinganine reductase

organism: Bos taurus

smiles: CCCCCCCCCCCCCCCC(=O)C(CO)N

enzyme: 3-dehydrosphinganine reductase


enzyme_display_name: L-threonine 3-dehydrogenase

organism: Capra hircus

substrate_name: NAD+

enzyme: L-threonine 3-dehydrogenase

substrate: NAD+


substrate_name: L-threonine

smiles: CC(C(C(=O)O)N)O

substrate: L-threonine


organism: Cupriavidus necator


assay_conditions_summary: pH 10; 303.15 K; cofactor NAD+

proof_lines: mutant enzyme T186N, at pH 10.0 and 30°C


proof_lines: mutant enzyme G184A, at pH 10.0 and 30°C


substrate_name: n-butanol

smiles: CCCCO

substrate: n-butanol


substrate_name: ethanol

smiles: CCO

substrate: ethanol


organism: Escherichia coli K-12

proof_lines:
metal-ion free enzyme
demetallized enzyme
enzyme activated with 0.07 mM Cd2+


organism: Staphylococcus aureus


organism: Sus scrofa


organism: Homo sapiens

assay_conditions_summary: pH 7.4; cofactor NADPH

proof_lines: wild type enzyme, in 100 mM sodium phosphate, pH 7.4


substrate_name: all-trans-retinol

substrate: all-trans-retinol


assay_conditions_summary: pH 7.4; cofactor NADP+


assay_conditions_summary: pH 7.4; cofactor NAD+


substrate_name: NADH

assay_conditions_summary: pH 7.4; cofactor NADH

substrate: NADH


substrate_name: all-trans-retinal

substrate: all-trans-retinal


substrate_name: androsterone

substrate: androsterone


assay_conditions_summary: pH 7.3; 310.15 K; cofactor NAD+


substrate_name: retinol bound to cellular retinol binding protein

proof_lines:
pH 7.3, 37°C
pH 7.3, 37°C, substrate 3alpha-adiol
pH 7.3, 37°C, substrate androsterone


proof_lines: pH not specified in the publication, temperature not specified in the publication


proof_lines:
pH 7.3, 37°C, substrate 3alpha-adiol
pH 7.3, 37°C, substrate androsterone


substrate_name: all-trans retinal

substrate: all-trans retinal


assay_conditions_summary: cofactor NADH


organism: Mus musculus

proof_lines: recombinant enzyme, pH and temperature not specified in the publication


assay_conditions_summary: cofactor NAD+


organism: Rattus norvegicus

substrate_name: retinol

substrate: retinol


enzyme_display_name: pyridoxal 4-dehydrogenase

organism: Pseudomonas sp.

substrate_name: pyridoxal

enzyme: pyridoxal 4-dehydrogenase

substrate: pyridoxal


enzyme_display_name: carnitine 3-dehydrogenase

enzyme: carnitine 3-dehydrogenase


substrate_name: L-carnitine

assay_conditions_summary: pH 9

proof_lines:
at pH 9.0
at pH 9.5

substrate: L-carnitine


organism: Pseudomonas putida

assay_conditions_summary: pH 7; cofactor NADH

proof_lines:
potassium phosphate buffer, pH 7
Tris-HCl buffer, pH 9


assay_conditions_summary: pH 7


enzyme_display_name: GDP-mannose 6-dehydrogenase

organism: Arthrobacter sp.

substrate_name: GDP-mannose

enzyme: GDP-mannose 6-dehydrogenase

substrate: GDP-mannose


substrate_name: deoxy-GDP-D-mannose

substrate: deoxy-GDP-D-mannose


substrate_name: GDP-D-mannose

proof_lines: pH 8.0, 5°C, recombinant wild-type enzyme

substrate: GDP-D-mannose


assay_conditions_summary: pH 8; 278.15 K; cofactor NAD+


substrate_name: Unknown

substrate: Unknown


substrate_name: NAD+

assay_conditions_summary: pH 8; 297.15 K; buffer cosubstrate GDP-D-mannose 24°C; cofactor NAD+

proof_lines:
pH 8.0, phosphate buffer
cosubstrate GDP-D-mannose
24°C, pH 8.0


enzyme_display_name: dTDP-4-dehydrorhamnose reductase

organism: Salmonella enterica

enzyme: dTDP-4-dehydrorhamnose reductase


substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

substrate: NADPH


enzyme_display_name: mannitol 2-dehydrogenase (NADP(+))

organism: Alternaria alternata

assay_conditions_summary: pH 7; cofactor NADPH

proof_lines: plusminus 0.0035 mM, pH 7.0, room temperature

enzyme: mannitol 2-dehydrogenase (NADP(+))


substrate_name: D-fructose

proof_lines: plusminus 80 mM, pH 7.0, room temperature

substrate: D-fructose


enzyme_display_name: sorbitol-6-phosphate 2-dehydrogenase

organism: Escherichia coli

enzyme: sorbitol-6-phosphate 2-dehydrogenase

substrate: NAD+


substrate_name: D-sorbitol 6-phosphate

substrate: D-sorbitol 6-phosphate


assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADH


substrate_name: D-fructose 6-phosphate

substrate: D-fructose 6-phosphate


substrate_name: prostaglandin E1

substrate: prostaglandin E1


organism: Canis lupus familiaris

proof_lines:
enzyme from kidney
kidney enzyme, pH 7.4, 37°C
enzyme from heart


assay_conditions_summary: pH 7.5; 310.15 K; cofactor NAD+


proof_lines:
37°C, pH 7.5
recombinant enzyme expressed in E. coli
at pH 9.0, + prostaglandin E2


proof_lines:
37°C, pH 7.5
substrate prostaglandin E1


substrate_name: prostaglandin A1

substrate: prostaglandin A1


proof_lines:
at pH 7.0
at pH 9.0
recombinant enzyme expressed in E. coli


substrate_name: Prostaglandin A2

substrate: Prostaglandin A2


substrate_name: prostaglandin E2

proof_lines: pH 8.0, 25°C, 20 nM [1-(3-methylphenyl)-1H-benzimidazol-5-yl](piperidin-1-yl)methanone

proof_lines: pH 8.0, 25°C, 10 nM 3-(2,5-dimethyl-1-phenyl-1H-pyrrol-3-yl)-6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,3-a]azepine

proof_lines: pH 8.0, 25°C, 10 nM [1-(3-methylphenyl)-1H-benzimidazol-5-yl](piperidin-1-yl)methanone

substrate: prostaglandin E2


substrate_name: prostaglandin F2alpha

substrate: prostaglandin F2alpha


smiles: CCCCCC(C=CC=CCC=CCCCC(=O)O)O


substrate_name: NADH

proof_lines:
at pH 7.0, + 15-keto-PGE1
at pH 7.0, + 15-ketoprostaglandin E2
at pH 9.0, + 15-ketoprostaglandin E2

substrate: NADH


substrate_name: 15-ketoprostaglandin E2

proof_lines:
at pH 9.0
at pH 7.0

substrate: 15-ketoprostaglandin E2


substrate_name: 15-ketoprostaglandin E1

substrate: 15-ketoprostaglandin E1


substrate_name: 6-oxo-prostaglandin F1alpha

substrate: 6-oxo-prostaglandin F1alpha


assay_conditions_summary: pH 7.4; 310.15 K; cofactor NAD+

proof_lines: prostaglandin E2 as the variable substrate


substrate_name: allopregnanolone

substrate: allopregnanolone


substrate_name: androstenediol

substrate: androstenediol


substrate_name: dehydroepiandrosterone

proof_lines: microsomes mitochondria

substrate: dehydroepiandrosterone


substrate_name: pregnenolone

proof_lines: mitochondria microsomes

substrate: pregnenolone


substrate_name: NAD+


substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

substrate: NADP+


substrate_name: progesterone

substrate: progesterone


substrate_name: testosterone

substrate: testosterone


substrate_name: 13,14-dihydro-15-keto-prostaglandin F2

substrate: 13,14-dihydro-15-keto-prostaglandin F2


substrate_name: androstenedione

substrate: androstenedione


substrate_name: D-glucose

substrate: D-glucose


substrate_name: prostaglandin E1

substrate: prostaglandin E1


substrate_name: D-glucuronic acid

substrate: D-glucuronic acid


substrate_name: D-xylose

substrate: D-xylose


substrate_name: Camphorquinone

substrate: Camphorquinone


substrate_name: caprinaldehyde

smiles: CCCCCCCCCC=O

substrate: caprinaldehyde


substrate_name: menadione

substrate: menadione


substrate_name: D-glyceraldehyde

smiles: C(C(C=O)O)O

substrate: D-glyceraldehyde


substrate_name: cyclohexanone

substrate: cyclohexanone


substrate_name: Phenylglyoxal

substrate: Phenylglyoxal


substrate_name: methylglyoxal

substrate: methylglyoxal


enzyme_display_name: 11beta-hydroxysteroid dehydrogenase

substrate_name: corticosterone

proof_lines: 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1

enzyme: 11beta-hydroxysteroid dehydrogenase

substrate: corticosterone


proof_lines: i.e. cortisol,temperature not specified in the publication, pH 9


proof_lines: i.e. cortisone, temperature not specified in the publication, pH 9


substrate_name: NADPH

assay_conditions_summary: pH 9; cofactor NADPH

proof_lines: temperature not specified in the publication, pH 9

substrate: NADPH


substrate_name: NADP+

assay_conditions_summary: pH 9; cofactor NADP+

substrate: NADP+


organism: Rattus sp.

proof_lines: reduction of corticosterone, ordered sequential mechanism


substrate_name: NAD+

substrate: NAD+


proof_lines:
at pH 7.0
at pH 8.5
isoform 11beta-HSD1


substrate_name: cortisol

assay_conditions_summary: 310.15 K; cofactor NADP+

proof_lines: enzyme from caput epididymidis, cosubstrate NADP+, 37°C, pH not specified in the publication

proof_lines: enzyme from testis, cosubstrate NADP+, 37°C, pH not specified in the publication

proof_lines: enzyme from bulbourethral gland, cosubstrate NADP+, 37°C, pH not specified in the publication

substrate: cortisol


enzyme_display_name: sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming)

organism: Drosophila melanogaster

substrate_name: sepiapterin

substrate: sepiapterin


assay_conditions_summary: pH 8.1; 303.15 K; cofactor NAD+


substrate_name: (S)-ureidoglycolate

substrate: (S)-ureidoglycolate


enzyme_display_name: 7alpha-hydroxysteroid dehydrogenase

substrate_name: cholic acid

enzyme: 7alpha-hydroxysteroid dehydrogenase

substrate: cholic acid


substrate_name: glycocholic acid

substrate: glycocholic acid


substrate_name: taurocholic acid

substrate: taurocholic acid


substrate_name: taurochenodeoxycholic acid

substrate: taurochenodeoxycholic acid


substrate_name: chenodeoxycholic acid

substrate: chenodeoxycholic acid


substrate_name: glycochenodeoxycholic acid

substrate: glycochenodeoxycholic acid


enzyme_display_name: 2-dehydropantoate 2-reductase

substrate_name: thio-NADPH

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

proof_lines:
pH 7.5, 25°C, recombinant enzyme
pH 7.6

enzyme: 2-dehydropantoate 2-reductase

substrate: thio-NADPH


smiles: CC(C)C(=O)C(=O)O


assay_conditions_summary: pH 7.5; 300.15 K; cofactor NADPH


substrate_name: ketopantoate

substrate: ketopantoate


proof_lines:
+/-0.009, mutant K176C, alkylated
+/-0.01, mutant K176C


substrate_name: Pantoate

substrate: Pantoate


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: ketopantoic acid

smiles: CC(C)(CO)C(=O)C(=O)O

substrate: ketopantoic acid


organism: Stenotrophomonas maltophilia


substrate_name: D-pantoic acid

smiles: CC(C)(CO)C(C(=O)O)O

assay_conditions_summary: pH 8

substrate: D-pantoic acid


enzyme_display_name: D-xylose 1-dehydrogenase

organism: Caulobacter vibrioides

enzyme: D-xylose 1-dehydrogenase


substrate_name: D-xylose

proof_lines:
at pH 7.0 and 25°C
25°C, pH 6.8
25°C, pH 9.0

substrate: D-xylose


substrate_name: NADH

assay_conditions_summary: pH 7; 298.15 K; cofactor NADH

substrate: NADH


substrate_name: NAD+

assay_conditions_summary: pH 6.8; 298.15 K; cofactor NAD+

substrate: NAD+


assay_conditions_summary: cofactor NAD+

proof_lines:
strain 89B
strain 90A


proof_lines:
strain 90A
strain 89B


enzyme_display_name: D-xylose 1-dehydrogenase (NADP(+), D-xylono-1,5-lactone-forming)

substrate_name: D-arabinose

smiles: C(C(C(C(C=O)O)O)O)O

substrate: D-arabinose


substrate_name: D-galactose

enzyme: D-xylose 1-dehydrogenase (NADP(+), D-xylono-1,5-lactone-forming)

substrate: D-galactose


smiles: C(C=O)C(C(C(CO)O)O)O


substrate_name: D-ribose

assay_conditions_summary: buffer context

substrate: D-ribose


substrate_name: NADP+

substrate: NADP+


organism: Trichoderma reesei

substrate_name: D-glucose

substrate: D-glucose


assay_conditions_summary: pH 8.1; 303.15 K; cofactor NADP+


enzyme_display_name: carbonyl reductase (NADPH)

substrate_name: daunorubicin

enzyme: carbonyl reductase (NADPH)

substrate: daunorubicin


substrate_name: menadione

substrate: menadione


substrate_name: all-trans-retinol

substrate: all-trans-retinol


assay_conditions_summary: pH 6


substrate_name: isatin

substrate: isatin


substrate_name: heptanophenone

substrate: heptanophenone


substrate_name: valerophenone

substrate: valerophenone


substrate_name: acenaphthenequinone

substrate: acenaphthenequinone


substrate_name: n-butyrophenone

substrate: n-butyrophenone


smiles: CCCCC(=O)C(=O)C


smiles: CCC(=O)C(=O)C


smiles: CCCC(=O)C(=O)C


substrate_name: hexanophenone

substrate: hexanophenone


substrate_name: benzoylformic acid ethyl ester

substrate: benzoylformic acid ethyl ester


organism: Cavia porcellus

substrate_name: pyridine 4-aldehyde

assay_conditions_summary: cofactor NADPH

proof_lines:
cofactor NADPH
cofactor NADH

substrate: pyridine 4-aldehyde


substrate_name: pyridine 3-aldehyde

proof_lines:
cofactor NADPH
cosubstrate NADH

substrate: pyridine 3-aldehyde


substrate_name: Benzalacetone

substrate: Benzalacetone


substrate_name: acetaldehyde

smiles: CC=O

substrate: acetaldehyde


substrate_name: acetone

smiles: CC(=O)C

substrate: acetone


substrate_name: crotonaldehyde

smiles: CC=CC=O

substrate: crotonaldehyde


organism: Cricetulus griseus

assay_conditions_summary: pH 6.5; 298.15 K; cofactor NADPH

proof_lines: at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C


substrate_name: NADPH

substrate: NADPH


substrate_name: NADH

substrate: NADH


substrate_name: menadione

substrate: menadione


organism: Gallus gallus


assay_conditions_summary: pH 7.4; 310.15 K; cofactor NADPH


substrate_name: estrone

substrate: estrone


substrate_name: testosterone

substrate: testosterone


substrate_name: prostaglandin E2

substrate: prostaglandin E2


substrate_name: isatin

substrate: isatin


substrate_name: p-toluquinone

substrate: p-toluquinone


organism: Homo sapiens

proof_lines: wild-type CBR3 and mutant V244M. Residues 262-277, 236-244/262-277 or 230 of CBR3 exchanged to the corresponding amino acids of CBR1


proof_lines: after treatment with S-nitrosoglutathione, pH 7.4, 25°C

proof_lines: enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C


proof_lines: residues 142-143, 230, 236-244, 270 of CBR3 exchanged to the corresponding amino acids of CBR1


substrate_name: oracin

substrate: oracin


substrate_name: daunorubicin

substrate: daunorubicin


proof_lines:
wild-type, pH 7.4, 25°C
wild-type, pH 7.4, 37°C


proof_lines:
wild-type, pH 7.4, 37°C
wild-type
wild-type, 37°C, pH 7.4


proof_lines: wild-type, pH 7.4, temperature not specified in the publication


substrate_name: doxorubicin

proof_lines: single nucleotide polymorphism V88I mutation

substrate: doxorubicin


proof_lines: single nucleotide polymorphism P131S mutation


substrate_name: benzoylpyridine

substrate: benzoylpyridine


substrate_name: Phenylglyoxal

substrate: Phenylglyoxal


smiles: CC(=O)C(=O)C


substrate_name: S-nitrosoglutathione

proof_lines: mutant C227S, pH 7.4, temperature not specified in the publication

substrate: S-nitrosoglutathione


proof_lines: mutant D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, pH 7.4, temperature not specified in the publication


proof_lines: mutant P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244PpH 7.4, temperature not specified in the publication


proof_lines: mutant Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S, pH 7.4, temperature not specified in the publication


substrate_name: Prostaglandin B2

substrate: Prostaglandin B2


substrate_name: 4-formylbenzoic acid

substrate: 4-formylbenzoic acid


proof_lines:
wild-type
wild-type, pH 7.4, 25°C
wild-type, pH 7.4, 37°C


proof_lines:
pH 6.5, 25°C
Km depending on enzyme form


substrate_name: prostaglandin E1

substrate: prostaglandin E1


proof_lines:
in stomach
in kidney
in colon


smiles: CCCCCC(=O)C=CC=O


organism: Oryctolagus cuniculus


substrate_name: loxoprofen

substrate: loxoprofen


substrate_name: acetohexamide

substrate: acetohexamide


substrate_name: befunolol

substrate: befunolol


substrate_name: NADPH

substrate: NADPH


organism: Rattus norvegicus


substrate_name: menadione

substrate: menadione


organism: Sus scrofa


substrate_name: hexaphenone

proof_lines:
pH 6.0, 25°C, recombinant enzyme
pH 6.0, recombinant enzyme
pH 6.0, 37°C

substrate: hexaphenone


substrate_name: heptanophenone

proof_lines:
pH 6.0, recombinant enzyme
pH 6.0, 37°C

substrate: heptanophenone


substrate_name: isatin

substrate: isatin


substrate_name: nonanophenone

substrate: nonanophenone


substrate_name: benzil

substrate: benzil


proof_lines:
pH 6.0, 25°C, recombinant enzyme
pH 6.0, recombinant enzyme


substrate_name: diacetyl

substrate: diacetyl


substrate_name: pyridin-4-yl hexanoate

substrate: pyridin-4-yl hexanoate


proof_lines:
pH 7.4, 37°C
pH 6.0, 25°C, recombinant enzyme
pH 6.0, recombinant enzyme


substrate_name: valerophenone

proof_lines:
pH 6.0, 37°C
pH 6.0, 25°C, recombinant enzyme
pH 6.0, recombinant enzyme

substrate: valerophenone


substrate_name: butyrophenone

substrate: butyrophenone


substrate_name: propiophenone

substrate: propiophenone


substrate_name: Adrenochrome

substrate: Adrenochrome


substrate_name: prostaglandin E2

substrate: prostaglandin E2


enzyme_display_name: prostaglandin-E2 9-reductase

organism: Bos taurus

enzyme: prostaglandin-E2 9-reductase


proof_lines: competitive inhibitor for the reduction of 20alpha-hydroxy-pregn-4-en-3-one to progesterone


substrate_name: DL-glyceraldehyde

substrate: DL-glyceraldehyde


substrate_name: methylglyoxal

smiles: CC(=O)C=O

substrate: methylglyoxal


enzyme_display_name: coniferyl-alcohol dehydrogenase

organism: Streptomyces sp.

substrate_name: coniferyl alcohol

enzyme: coniferyl-alcohol dehydrogenase

substrate: coniferyl alcohol


enzyme_display_name: cinnamyl-alcohol dehydrogenase

organism: Arabidopsis thaliana

proof_lines: mutant T49A, 30°C, pH not specified in the publication

enzyme: cinnamyl-alcohol dehydrogenase


proof_lines: wild-type, 30°C, pH not specified in the publication


proof_lines: mutant H52A, 30°C, pH not specified in the publication


proof_lines: mutant D57A, 30°C, pH not specified in the publication


organism: Medicago truncatula

substrate_name: sinapyl aldehyde

proof_lines: Mt-CAD2, pH and temperature not specified in the publication

substrate: sinapyl aldehyde


proof_lines: Mt-CAD2 mutant Y136F, pH and temperature not specified in the publication


substrate_name: coniferyl aldehyde

substrate: coniferyl aldehyde


substrate_name: p-coumaryl aldehyde

substrate: p-coumaryl aldehyde


proof_lines: wild-type Mt-CAD2, pH and temperature not specified in the publication


organism: Oryza sativa

proof_lines: pH 8.8, 30°C, recombinant enzyme encoded by GH2


organism: Oryza sativa Japonica Group


substrate_name: 4-coumaryl aldehyde

substrate: 4-coumaryl aldehyde


substrate_name: cinnamyl aldehyde

substrate: cinnamyl aldehyde


organism: Panicum virgatum

proof_lines: pH not specified in the publication, temperature not specified in the publication


substrate_name: coumaryl alcohol

substrate: coumaryl alcohol


substrate_name: sinapyl alcohol

substrate: sinapyl alcohol


organism: Pinus taeda

substrate_name: coniferaldehyde

substrate: coniferaldehyde


substrate_name: NADPH

proof_lines:
with coniferaldehyde
with cinnamaldehyde

substrate: NADPH


substrate_name: cinnamaldehyde

substrate: cinnamaldehyde


substrate_name: sinapaldehyde

substrate: sinapaldehyde


organism: Sorghum bicolor

proof_lines: isoform CAD4 mutant Y288P, at pH 6.5 and 30°C


proof_lines: wild type isoform CAD4, at pH 6.5 and 30°C

proof_lines: wild type isoform CAD2, at pH 6.5 and 30°C


proof_lines: isoform CAD4 mutant W58L, at pH 6.5 and 30°C


proof_lines: isoform CAD4 mutant Y95V, at pH 6.5 and 30°C


proof_lines: isoform CAD4 mutant G301F, at pH 6.5 and 30°C


proof_lines: isoform CAD4 mutant L119W/G301F, at pH 6.5 and 30°C


proof_lines: pH 8.8, temperature not specified in the publication


organism: Triticum aestivum

proof_lines: recombinant GST-tagged enzyme, pH and temperature not specified in the publication


substrate_name: 5-hydroxyconiferyl aldehyde

substrate: 5-hydroxyconiferyl aldehyde


substrate_name: caffeoyl aldehyde

substrate: caffeoyl aldehyde


substrate_name: p-coumaryl alcohol

proof_lines:
isoform CAD-C
purified isoform CAD-C
isoform CAD-B

substrate: p-coumaryl alcohol


proof_lines:
purified isoform CAD-C
isoform CAD-B
isoform CAD-A


proof_lines:
purified isoform CAD-C
isoform CAD-C
isoform CAD-B


substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

substrate: NADP+


substrate_name: Prostaglandin D2

substrate: Prostaglandin D2


substrate_name: prostaglandin D1

substrate: prostaglandin D1


substrate_name: prostaglandin E2

substrate: prostaglandin E2


substrate_name: prostaglandin F2alpha

substrate: prostaglandin F2alpha


substrate_name: prostaglandin I2

substrate: prostaglandin I2


enzyme_display_name: 1,3-propanediol dehydrogenase

organism: Citrobacter freundii

substrate_name: NADH

assay_conditions_summary: cofactor NADH

enzyme: 1,3-propanediol dehydrogenase

substrate: NADH


substrate_name: NAD+

substrate: NAD+


smiles: C(CO)CO


substrate_name: propionaldehyde

smiles: CCC=O

substrate: propionaldehyde


smiles: C(CO)C=O

proof_lines: pH and temperature not specified in the publication


organism: Clostridium butyricum

assay_conditions_summary: pH 9; 310.15 K; cofactor NADH

proof_lines: with propionaldehyde as cosubstrate, at pH 9.0 and 37°C


assay_conditions_summary: pH 11; 303.15 K; cofactor NAD+

proof_lines: with propane-1,3-diol as cosubstrate, at pH 11.0 and 30°C


organism: Klebsiella pneumoniae

substrate_name: Valeraldehyde

smiles: CCCCC=O

substrate: Valeraldehyde


substrate_name: formaldehyde

smiles: C=O

substrate: formaldehyde


substrate_name: acetaldehyde

substrate: acetaldehyde


substrate_name: Butyraldehyde

smiles: CCCC=O

substrate: Butyraldehyde


assay_conditions_summary: pH 7; 310.15 K; cofactor NADH


substrate_name: glycerol

smiles: C(C(CO)O)O

substrate: glycerol


assay_conditions_summary: pH 7.4; 310.15 K; cofactor NADP+


assay_conditions_summary: pH 7.4; 310.15 K; cofactor NAD+


assay_conditions_summary: pH 7.4; 310.15 K; cofactor NADH


substrate_name: NADPH

substrate: NADPH


assay_conditions_summary: pH 9; 328.15 K; cofactor NAD+

proof_lines:
pH 9.0, 55°C
pH 9.5, 30°C, recombinant enzyme


enzyme_display_name: uronate dehydrogenase

organism: Agrobacterium tumefaciens

substrate_name: beta-D-glucuronate

enzyme: uronate dehydrogenase

substrate: beta-D-glucuronate


proof_lines: mutant enzyme H101N/H236K, at pH 8.0 and 25°C


proof_lines: mutant enzyme A41P/H101Y/H236K, at pH 8.0 and 25°C


proof_lines: mutant enzyme A41P/H101N/H236K, at pH 8.0 and 25°C


proof_lines: mutant enzyme H101Y/H236K, at pH 8.0 and 25°C


proof_lines: mutant enzyme A41P/H101Y/H236R, at pH 8.0 and 25°C


proof_lines: mutant enzyme A41P/H101N/H236R, at pH 8.0 and 25°C


proof_lines: mutant enzyme A41P/H236K, at pH 8.0 and 25°C


substrate_name: D-glucuronic acid

substrate: D-glucuronic acid


proof_lines: mutant enzyme A39P/H99Y, at pH 8.0 and 30°C


proof_lines: mutant enzyme A39P/H234K, at pH 8.0 and 30°C


proof_lines: mutant enzyme H99Y/H234K, at pH 8.0 and 30°C


proof_lines: mutant enzyme A39P/H99Y/H234K, at pH 8.0 and 30°C


substrate_name: beta-D-galacturonate

assay_conditions_summary: buffer recombinant His-tagged enzyme

proof_lines: recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer

proof_lines: recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer

substrate: beta-D-galacturonate


assay_conditions_summary: buffer potassium phosphate; cofactor NAD+


substrate_name: D-galacturonate

substrate: D-galacturonate


organism: Pseudomonas fluorescens


assay_conditions_summary: pH 8; 298.15 K; cofactor NAD+


organism: Pseudomonas syringae


assay_conditions_summary: cofactor NAD+


enzyme_display_name: IMP dehydrogenase

organism: Bacillus amyloliquefaciens

assay_conditions_summary: pH 8; 313.15 K; cofactor NAD+

proof_lines: pH 8.0, 40°C, recombinant wild-type enzyme and mutant K481E

proof_lines: pH 8.0, 40°C, recombinant wild-type enzyme and mutant P166S

proof_lines: pH 8.0, 40°C, recombinant wild-type enzyme and mutant IMPDHDELTACBS

enzyme: IMP dehydrogenase


substrate_name: IMP

proof_lines: pH 8.0, 40°C, recombinant wild-type enzyme and mutant P166S and K481E

proof_lines: pH 8.0, 40°C, recombinant wild-type enzyme, and recombinant mutant enzymes, P166S, K481E, and IMPDHDELTACBS

substrate: IMP


proof_lines: pH 8.0, 40°C, recombinant mutant enzyme IMPDHDELTACBS

proof_lines: pH 8.0, 40°C, recombinant mutant IMPDHDELTACBS


organism: Bacillus anthracis

proof_lines: pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL


proof_lines: pH 8.0, 25°C, recombinant wild-type enzyme


proof_lines: pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS


mutation_signature: recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL


mutation_signature: recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS


proof_lines: pH 8.0, 30°C, recombinant enzyme, without MgATP2-

proof_lines: pH 8.0, 30°C, recombinant enzyme, with MgATP2-


substrate_name: Unknown

proof_lines: substrate inosine monophosphate, pH 8.0, 22°C

substrate: Unknown


substrate_name: Inosine 5'-diphosphate

substrate: Inosine 5'-diphosphate


organism: Bacillus subtilis


substrate_name: K+

substrate: K+


organism: Campylobacter jejuni


proof_lines: pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS


mutation_signature: recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS


organism: Candida albicans


organism: Clostridium perfringens

proof_lines: pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant ClpIMPDHDELTAL


mutation_signature: recombinant short enzyme CBS-deletion mutant ClpIMPDHDELTAL


organism: Cricetulus griseus


organism: Escherichia coli


proof_lines: chemically synthesized thiazole-4-carboxamide adenine dinucleotide

proof_lines: with thiazole-4-carboxamide adenine dinucleotide synthesized chemically

proof_lines: enzymatically synthesized thiazole-4-carboxamide adenine dinucleotide


substrate_name: inosine 5'-phosphate

substrate: inosine 5'-phosphate


organism: Homo sapiens

substrate_name: NAD+

assay_conditions_summary: pH 8; 310.15 K; cofactor NAD+

proof_lines: pH 8.0, 37°C, recombinant wild-type enzyme

substrate: NAD+


substrate_name: beta-NAD+

proof_lines: pH 8.0, 37°C, recombinant mutants D226N and V268I

substrate: beta-NAD+


assay_conditions_summary: pH 8; 303.15 K; cofactor NAD+


organism: Leishmania donovani


assay_conditions_summary: pH 7.5; 298.15 K; cofactor NAD+


organism: Mus musculus

proof_lines: pH not specified in the publication, temperature not specified in the publication


proof_lines:
for the competitive component
for the non-competitive component
pH 8.0, 37°C


organism: Mycobacterium tuberculosis


assay_conditions_summary: pH 8.5; 310.15 K; cofactor NAD+

proof_lines:
recombinant enzyme, pH 8.5, 37°C
pH 8.0, 25°C


proof_lines: pH and temperature not specified in the publication


proof_lines: recombinant wild-type enzyme, pH 8.0, 22°C


assay_conditions_summary: pH 8; 295.15 K; cofactor NAD+


proof_lines: recombinant MtbIMPDH2DELTACBS mutant enzyme, pH 8.0, 22°C


organism: Neisseria meningitidis


organism: Penicillium brevicompactum

proof_lines: wild type enzyme isoform IMPDHA, at pH 8.0 and 25°C


organism: Rattus norvegicus


proof_lines:
versus inosine 5'-phosphate
versus NAD+


organism: Staphylococcus aureus

proof_lines: pH 8.0, 30°C, recombinant enzyme, without MgATP2-

proof_lines: pH 8.0, 30°C, recombinant enzyme, with MgATP2-


organism: Streptococcus pyogenes


organism: Sus scrofa


organism: Toxoplasma gondii


organism: Trypanosoma brucei


substrate_name: androsterone

substrate: androsterone


substrate_name: testosterone

substrate: testosterone


proof_lines:
wild-type
wild-type, pH 7.0, 25°C


substrate_name: Epitestosterone

substrate: Epitestosterone


assay_conditions_summary: cofactor NADP+


substrate_name: glycolithocholic acid

substrate: glycolithocholic acid


substrate_name: NADP+

substrate: NADP+


substrate_name: cis-benzene dihydrodiol

substrate: cis-benzene dihydrodiol


substrate_name: Naphthalene dihydrodiol

substrate: Naphthalene dihydrodiol


substrate_name: lithocholic acid

substrate: lithocholic acid


organism: Bos taurus

substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

substrate: NADPH


enzyme_display_name: dihydroflavanol 4-reductase

organism: Camellia sinensis

substrate_name: (+)-dihydroquercetin

enzyme: dihydroflavanol 4-reductase

substrate: (+)-dihydroquercetin


substrate_name: (+)-dihydromyricetin

substrate: (+)-dihydromyricetin


substrate_name: (+)-dihydrokaempferol

substrate: (+)-dihydrokaempferol


substrate_name: estrone

substrate: estrone


substrate_name: Unknown

assay_conditions_summary: pH 7

proof_lines: pH 7.0, temperature not specified in the publication

substrate: Unknown


organism: Phascolarctos cinereus

proof_lines: enzyme avtivity in hepatic microsomes, one individual animal


enzyme_display_name: methanol dehydrogenase

organism: Bacillus methanolicus

assay_conditions_summary: pH 9.5; 323.15 K; cofactor NAD+

proof_lines: recombinant enzyme without added Mg2+, pH 9.5, 50°C

enzyme: methanol dehydrogenase


substrate_name: methanol

smiles: CO

substrate: methanol


proof_lines: recombinant enzyme, pH 9.0, 30°C, in presence of activator Act

proof_lines: recombinant enzyme, pH 7.4, 30°C, in presence of activator Act

proof_lines: recombinant enzyme, pH 9.0, 30°C, in absence of activator Act


substrate_name: ethanol

smiles: CCO

substrate: ethanol


assay_conditions_summary: pH 7.4; 303.15 K; cofactor NADP+

proof_lines: recombinant enzyme mutant D38G, pH 7.4, 30°C, in presence of activator Act, with NADP+

proof_lines: recombinant enzyme mutant D38G, pH 7.4, 30°C, in absence of activator Act, with NADP+


proof_lines: recombinant enzyme mutant S98G, pH 7.4, 30°C, in presence of activator Act

proof_lines: recombinant enzyme mutant S98G, pH 7.4, 30°C, in absence of activator Act


enzyme_display_name: codeinone reductase (NADPH)

organism: Papaver somniferum

substrate_name: codeine

assay_conditions_summary: pH 9; 303.15 K; cofactor NADPH

proof_lines:
pH 9.0, 30°C
enzyme from cell culture
isoenzymes from capsule tissue

enzyme: codeinone reductase (NADPH)

substrate: codeine


substrate_name: codeinone

assay_conditions_summary: pH 6.8; 303.15 K; cofactor NADPH

proof_lines:
pH 6.8, 30°C
isoenzymes from capsule tissue
enzyme from cell culture

substrate: codeinone


assay_conditions_summary: pH 9; 303.15 K; cofactor NADP+


substrate_name: Hydrocodone

proof_lines:
isoenzymes from capsule tissue
morphine, enzyme from cell culture
enzyme from cell culture

substrate: Hydrocodone


substrate_name: Hydromorphone

proof_lines:
isoenzymes from capsule tissue
enzyme from cell culture

substrate: Hydromorphone


substrate_name: morphine

substrate: morphine


substrate_name: Dihydrocodeine

substrate: Dihydrocodeine


enzyme_display_name: glyoxylate reductase

organism: Paracoccus sp. (in: a-proteobacteria)

substrate_name: glyoxylate

proof_lines: pH 7.0, 30°C, recombinant E141N/Q313E mutant formate dehydrogenase

enzyme: glyoxylate reductase

substrate: glyoxylate


organism: Rhizobium etli

substrate_name: Hydroxypyruvate

substrate: Hydroxypyruvate


substrate_name: phenylpyruvate

substrate: phenylpyruvate


smiles: CCC(=O)C(=O)O


organism: Escherichia coli


enzyme_display_name: 1,5-anhydro-D-fructose reductase

enzyme: 1,5-anhydro-D-fructose reductase


enzyme_display_name: L-idonate 5-dehydrogenase [NAD(P)(+)]

substrate_name: L-Idonate

smiles: C(C(C(C(C(C(=O)O)O)O)O)O)O

enzyme: L-idonate 5-dehydrogenase [NAD(P)(+)]

substrate: L-Idonate


organism: Arabidopsis thaliana

substrate_name: NADP+

assay_conditions_summary: pH 8; 310.15 K; cofactor NADP+

proof_lines: pH 8.0, 37°C, recombinant pseudomature enzyme

substrate: NADP+


substrate_name: 2-C-methyl-D-erythritol 4-phosphate

smiles: CC(CO)(C(COP(=O)(O)O)O)O

substrate: 2-C-methyl-D-erythritol 4-phosphate


substrate_name: 1-deoxy-D-xylulose 5-phosphate

smiles: CC(=O)C(C(COP(=O)(O)O)O)O

substrate: 1-deoxy-D-xylulose 5-phosphate


substrate_name: NADPH

assay_conditions_summary: pH 8; 310.15 K; cofactor NADPH

substrate: NADPH


substrate_name: Unknown

substrate: Unknown


proof_lines: pH 7.6, 37°C, recombinant His-tagged enzyme


substrate_name: 1-fluoro-1-deoxy-D-xylulose 5-phosphate

assay_conditions_summary: pH 7.6; 310.15 K; cofactor NADPH

proof_lines: pH 7.5, 22°C, IC50 versus proliferation of Plasmodium falciparum strain Dd2

proof_lines: pH 7.5, 22°C, IC50 versus proliferation of Plasmodium falciparum strain Dd4

substrate: 1-fluoro-1-deoxy-D-xylulose 5-phosphate


proof_lines:
pH 7.6, 37°C, recombinant enzyme
with 1 mM MnCl2
with 1 mM CoCl2


smiles: CC(C(C(COP(=O)(O)O)O)O)O

proof_lines:
pH 7.7, presence of 3 mM MgCl2
pH 7.7, presence of 1 mM MnCl2


organism: Francisella tularensis

assay_conditions_summary: pH 7.8; 295.15 K; cofactor NADPH


organism: Mycobacterium tuberculosis


proof_lines: pH 7.9, 30°C, recombinant His-tagged enzyme


assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADP+

proof_lines: pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate


assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

proof_lines:
pH 7.5, 25°C, recombinant enzyme
presence of Co2+, pH 7.5, 25°C
presence of Mn2+, pH 7.5, 25°C


smiles: CC(=O)C(CO)O

proof_lines: pH 7.5, 25°C, recombinant enzyme in presence of phosphite dianion

proof_lines: pH 7.5, 25°C, recombinant enzyme in absence of phosphite dianion

proof_lines: pH 7.5, 25°C, recombinant enzyme in absence or presence of phosphite dianion


proof_lines: cosubstrate NADPH, presence of Co2+, pH 7.5, 25°C

proof_lines: cosubstrate NADPH, presence of Mg2+, pH 7.5, 25°C


substrate_name: NADH

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADH

proof_lines:
presence of Co2+, pH 7.5, 25°C
presence of Mn2+, pH 7.5, 25°C
presence of Mg2+, pH 7.5, 25°C

substrate: NADH


organism: Plasmodium falciparum


enzyme_display_name: 3beta-hydroxysteroid 3-dehydrogenase

organism: Homo sapiens

substrate_name: NAD+

assay_conditions_summary: pH 7.4; 300.15 K; cofactor NAD+

enzyme: 3beta-hydroxysteroid 3-dehydrogenase

substrate: NAD+


substrate_name: dehydroepiandrosterone

substrate: dehydroepiandrosterone


proof_lines:
3beta-HSD1 wild-type, pH 7.4, 27°C
pH 7.4, 27°C, wild-type enzyme
3beta-HSD2 wild-type, pH 7.4, 27°C


proof_lines: pH 7.0, 37°C, radiometric analysis, formation of 3beta-androstanediol

proof_lines: pH 7.0, 37°C, radiometric analysis, formation of 3alpha-androstanediol

proof_lines: pH 7.0, 37°C, spectrophotometric analysis, formation of 3beta-androstanediol or 3alpha-androstanediol


organism: Rattus norvegicus

proof_lines:
solubilized enzyme
microsomal-bound enzyme


enzyme_display_name: GDP-L-fucose synthase

enzyme: GDP-L-fucose synthase


assay_conditions_summary: cofactor NADH


organism: Escherichia coli K-12


proof_lines: mutant C109S, isotope [5''-2H]-GDP-6-deoxy-4-keto-mannose

proof_lines: mutant C109S, isotope [3''-2H]-GDP-6-deoxy-4-keto-mannose


organism: Helicobacter pylori


organism: Sus scrofa


smiles: CCOC(=O)C(C)C(=O)C

proof_lines: pH 7.0, 30°C, with a cofactor level 20fold higher than the Km value


substrate_name: methylglyoxal

smiles: CC(=O)C=O

proof_lines: YqhE YafB

substrate: methylglyoxal


enzyme_display_name: serine 3-dehydrogenase (NADP(+))

organism: Agrobacterium tumefaciens

enzyme: serine 3-dehydrogenase (NADP(+))


substrate_name: L-serine

smiles: C(C(C(=O)O)N)O

substrate: L-serine


substrate_name: L-Glycerate

smiles: C(C(C(=O)O)O)O

substrate: L-Glycerate


substrate_name: D-glycerate

substrate: D-glycerate


proof_lines:
versus L-serine, pH 9.0, 30°C
versus NADP+, pH 9.0, 30°C


enzyme_display_name: 4-phosphoerythronate dehydrogenase

proof_lines: in 50 mM Tris-HCl (pH 8.0) with 50 mM NaCl, 1 mM dithiothreitol, at 22°C

enzyme: 4-phosphoerythronate dehydrogenase


substrate_name: oxaloacetic acid

substrate: oxaloacetic acid


substrate_name: pyruvate

substrate: pyruvate


proof_lines: pH 8.0, 22°C, recombinant enzyme, versus 2-oxoglutarate

proof_lines: pH 8.0, 22°C, recombinant enzyme, versus 4-phospho-D-erythronate


enzyme_display_name: NADP-retinol dehydrogenase

organism: Danio rerio

substrate_name: all-trans-retinal

enzyme: NADP-retinol dehydrogenase

substrate: all-trans-retinal


substrate_name: estrone

substrate: estrone


organism: Drosophila melanogaster

proof_lines: Km-value is determined for microsomal preparations expressing recombinant Drosophila proteins


substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

proof_lines:
membrane-bound wild-type
wild-type

substrate: NADP+


assay_conditions_summary: pH 7.4; 310.15 K; cofactor NADPH

proof_lines: pH 7.4, 37°C, determined with all-trans-retinal


assay_conditions_summary: cofactor NADPH


substrate_name: all-trans-retinol

substrate: all-trans-retinol


assay_conditions_summary: pH 7.4; 310.15 K; cofactor NADP+

proof_lines: pH 7.4, 37°C, determined with all-trans-retinol


proof_lines:
37°C
purified recombinant enzyme
pH 7.4, 37°C


assay_conditions_summary: pH 7.4; 310.15 K; cofactor NADH

proof_lines: pH and temperature not specified in the publication. The apparent Km value for NADH is three orders of magnitude higher than that for NADPH


assay_conditions_summary: pH 7.4; 310.15 K; cofactor NAD+


proof_lines:
wild-type
membrane-bound wild-type
wild-type, pH 7.3, 37°C


enzyme_display_name: UDP-glucuronic acid oxidase (UDP-4-keto-hexauronic acid decarboxylating)

substrate_name: UDP-glucuronate

proof_lines: pH 7.5, 30°C, S433A decarboxylase mutant

substrate: UDP-glucuronate


proof_lines: pH 7.5, 30°C, E434A decarboxylase mutant

enzyme: UDP-glucuronic acid oxidase (UDP-4-keto-hexauronic acid decarboxylating)


assay_conditions_summary: pH 7.5; 303.15 K; cofactor NAD+


proof_lines: pH 7.5, 30°C, E434A decarboxylase mutant, E434Q decarboxylase mutant


proof_lines:
pH 8.0, 37°C, C-terminal domain of ArnA
pH 8.0, 37°C, full-length enzyme
pH 7.5, 30°C, ArnA decarboxylase domain


assay_conditions_summary: pH 8; 310.15 K; cofactor NAD+


enzyme_display_name: D-xylose reductase [NAD(P)H]

organism: Candida tropicalis

substrate_name: D-xylose

proof_lines: native enzyme; assay note present but not identity-resolving

proof_lines: recombinant enzyme; assay note present but not identity-resolving

enzyme: D-xylose reductase [NAD(P)H]

substrate: D-xylose


substrate_name: NADPH

assay_conditions_summary: pH 5.5; 318.15 K; cofactor NADPH

proof_lines: recombinant enzyme, pH not specified in the publication, temperature not specified in the publication

proof_lines: native enzyme, pH not specified in the publication, temperature not specified in the publication

substrate: NADPH


assay_conditions_summary: pH 5.5; 318.15 K; cofactor NADH


organism: Rasamsonia emersonii

assay_conditions_summary: pH 6.5; 310.15 K; cofactor NADPH

proof_lines: pH 6.5, 37°C, cosubstrate: NADH, wild-type enzyme

proof_lines: pH 6.5, 37°C, cosubstrate: NADPH, wild-type enzyme

proof_lines: wild-type, cosubstrate NADPH, pH 6.5, 37°C


assay_conditions_summary: pH 6.5; 310.15 K; cofactor NADH

proof_lines:
wild-type, pH 6.5, 37°C
pH 6.5, 37°C, wild-type enzyme


proof_lines: mutant K271R/N273, cosubstrate NADH, pH 6.5, 37°C

proof_lines: mutant K271R/N273, cosubstrate NADPH, pH 6.5, 37°C


enzyme_display_name: benzil reductase [(S)-benzoin forming]

organism: Bacillus cereus

substrate_name: benzil

enzyme: benzil reductase [(S)-benzoin forming]

substrate: benzil


substrate_name: UDP-N-acetyl-alpha-D-mannosamine

proof_lines: pH 10.0, 37°C, presence of 1 mM N-acetylglucosamine 1-phosphate

substrate: UDP-N-acetyl-alpha-D-mannosamine


substrate_name: NAD+

assay_conditions_summary: pH 10; 310.15 K; cofactor NAD+

substrate: NAD+


organism: Methanococcus maripaludis

assay_conditions_summary: pH 8.5; 310.15 K; cofactor NAD+


enzyme_display_name: 2-dehydropantolactone reductase

organism: Candida orthopsilosis

assay_conditions_summary: pH 7; 303.15 K; cofactor NADPH

enzyme: 2-dehydropantolactone reductase


organism: Candida parapsilosis

substrate_name: isatin

substrate: isatin


proof_lines:
pH 7.0, 30°C
pH 7.0, 30°C, substrate: isatin


substrate_name: Unknown

substrate: Unknown


enzyme_display_name: glucose-6-phosphate 3-dehydrogenase

organism: Bacillus subtilis

substrate_name: D-glucose 6-phosphate

smiles: C(C(C(C(C(C=O)O)O)O)O)OP(=O)(O)O

proof_lines: pH 9.5, 25°C, recombinant enzyme NtdC coupled to enzyme NtdA

enzyme: glucose-6-phosphate 3-dehydrogenase

substrate: D-glucose 6-phosphate


assay_conditions_summary: pH 9.5; 298.15 K; cofactor NAD+


enzyme_display_name: (S)-malate dehydrogenase (NAD(+), oxaloacetate-forming)

organism: Acinetobacter lwoffii

substrate_name: NADH

assay_conditions_summary: cofactor NADH

substrate: NADH


substrate_name: oxaloacetate

enzyme: (S)-malate dehydrogenase (NAD(+), oxaloacetate-forming)

substrate: oxaloacetate


substrate_name: L-malate

substrate: L-malate


assay_conditions_summary: cofactor NAD+


organism: Ananas comosus

assay_conditions_summary: pH 7.5; cofactor NADH


assay_conditions_summary: pH 7.5; cofactor NAD+


organism: Arabidopsis thaliana

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADH

proof_lines: oxidized isoform cytMDH1, at pH 7.5 and 25°C

proof_lines: reduced isoform cytMDH1, at pH 7.5 and 25°C


organism: Archaeoglobus fulgidus

assay_conditions_summary: pH 8; 338.15 K; cofactor NADH

proof_lines: pH and temperature not specified in the publication


substrate_name: (S)-malate

substrate: (S)-malate


organism: Aspergillus niger

proof_lines:
mitochondrial MDH
cyt MDH


proof_lines:
mit MDH
cytosolic MDH


organism: Bos taurus


substrate_name: phenylpyruvate

substrate: phenylpyruvate


organism: Candida albicans

assay_conditions_summary: pH 7.6; buffer 50 mM Tris-HCl

proof_lines: in 50 mM Tris-HCl buffer (pH 7.6), 1 mM MgCl2


organism: Canis lupus familiaris


substrate_name: Indolepyruvate

substrate: Indolepyruvate


organism: Drosophila virilis

proof_lines:
cytoplasmic enzyme
mitochondrial enzyme


organism: Echinococcus granulosus

assay_conditions_summary: pH 8

proof_lines: pH 8.0, recombinant protein fusions with glutathione S-transferase

proof_lines: pH 8.0, recombinant enzyme with a poly-His tail


assay_conditions_summary: pH 8; cofactor NADH

proof_lines: pH 8.0, recombinant enzyme with a poly-His tai


assay_conditions_summary: pH 7.6; cofactor NAD+

proof_lines: pH 7.6, recombinant enzyme with a poly-His tail

proof_lines: pH 7.6, recombinant protein fusions with glutathione S-transferase


proof_lines: pH 7.6, native enzyme, recombinant protein fusions with glutathione S-transferase


organism: Escherichia coli


proof_lines: pH 7.5, 30°C, recombinant wild-type enzyme

proof_lines: pH 7.5, 30°C,recombinant wild-type enzyme


organism: Glaesserella parasuis


substrate_name: 3-acetylpyridine adenine dinucleotide

substrate: 3-acetylpyridine adenine dinucleotide


substrate_name: Thionicotinamide adenine dinucleotide

substrate: Thionicotinamide adenine dinucleotide


substrate_name: pyridine 3-aldehyde adenine dinucleotide


substrate_name: nicotinamide hypoxanthine dinucleotide

substrate: nicotinamide hypoxanthine dinucleotide


substrate_name: malate

substrate: malate


organism: Malus domestica

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NAD+


assay_conditions_summary: pH 7.8; 303.15 K; cofactor NADH


organism: Moritella sp.

proof_lines:
pH 7.0, 30°C, recombinant enzyme
pH 7.0, 30°C


assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADH

proof_lines: enzyme from strain 5710, at pH 7.5 and 30°C


organism: Mycobacterium tuberculosis

proof_lines: recombinant enzyme, pH and temperature not specified in the publication


organism: Phytophthora infestans

proof_lines: recombinant wild type mitochondrial MDH, at 25°C

proof_lines: native wild type mitochondrial MDH, at 25°C

proof_lines: recombinant wild type cytosolic MDH, at 25°C


assay_conditions_summary: 298.15 K; cofactor NADH


assay_conditions_summary: 298.15 K; cofactor NAD+


organism: Rasamsonia emersonii

assay_conditions_summary: pH 7.4; 303.15 K; cofactor NAD+

proof_lines:
pH 7.4, 30°C, native enzyme
pH 7.4, 30°C, recombinant enzyme
native enzyme, at pH 7.4 and 30°C


proof_lines:
pH 7.4, 30°C, native enzyme
pH 7.4, 30°C, recombinant enzyme


proof_lines:
pH 7.4, 30°C, recombinant enzyme
pH 7.4, 30°C, native enzyme
native enzyme, at pH 7.4 and 30°C


assay_conditions_summary: pH 7.4; 303.15 K; cofactor NADH


organism: Rattus norvegicus

proof_lines:
normal rats
ischemic rats


assay_conditions_summary: cofactor NADH


organism: Rhodopseudomonas palustris

assay_conditions_summary: pH 8; 298.15 K; cofactor NADH

proof_lines:
pH 8.0, 25°C, tetrameric enzyme form
pH 8.0, 25°C, dimeric enzyme form


assay_conditions_summary: pH 9; 298.15 K; cofactor NAD+

proof_lines:
pH 9.0, 25°C, dimeric enzyme form
pH 9.0, 25°C, tetrameric enzyme form


organism: Saccharomyces cerevisiae

proof_lines: fusion protein of MDH with mitochondrial citrate synthase

proof_lines: in 50 mM Tris-HCl buffer (pH 7.6), 1 mM MgCl2


assay_conditions_summary: cofactor NAD+


organism: Salinibacter ruber

assay_conditions_summary: pH 8; 333.15 K; cofactor NADH


organism: Spinacia oleracea

proof_lines:
chloroplast envelope enzyme fraction
plasma membrane-bound enzyme fraction
chloroplast stroma enzyme fraction


enzyme_display_name: leucine dehydrogenase

organism: Geobacillus stearothermophilus

substrate_name: L-norvaline

smiles: CCCC(C(=O)O)N

enzyme: leucine dehydrogenase

substrate: L-norvaline


substrate_name: L-norleucine

smiles: CCCCC(C(=O)O)N

substrate: L-norleucine


substrate_name: L-Ile

smiles: CCC(C)C(C(=O)O)N

substrate: L-Ile


smiles: CCCCC(=O)C(=O)[O-]


enzyme_display_name: glucokinase

substrate_name: ATP

assay_conditions_summary: cofactor ATP

enzyme: glucokinase

substrate: ATP


enzyme_display_name: aspartate 1-decarboxylase

organism: Pyrococcus furiosus

substrate_name: L-Aspartate

source_db: oed

protein_accession_database: UniProt

kcat_over_km_origin: calculated

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

data_origin: experimental

enzyme: aspartate 1-decarboxylase

substrate: L-Aspartate

source: oed


enzyme_display_name: Bifunctional aspartate aminotransferase and L-aspartate beta-decarboxylase

organism: Comamonas testosteroni

substrate_name: L-aspartate

source_db: uniprot

substrate: L-aspartate

source: uniprot


enzyme_display_name: UniProt P69910

substrate_name: L-glutamate

source_db: skid

source_license: CC BY-NC-ND 4.0

smiles: C(CC(=O)O)C(C(=O)O)N

enzyme: UniProt P69910

substrate: L-glutamate

source: skid


enzyme_display_name: glutamate decarboxylase

enzyme: glutamate decarboxylase


enzyme_display_name: aspartate 4-decarboxylase

organism: [Pseudomonas] dacunhae

enzyme: aspartate 4-decarboxylase


organism: Alcaligenes faecalis

substrate_name: erythro-beta-hydroxyaspartate

substrate: erythro-beta-hydroxyaspartate


substrate_name: threo-beta-hydroxyaspartate

substrate: threo-beta-hydroxyaspartate


substrate_name: L-Asp

substrate: L-Asp


substrate_name: aminomalonate

substrate: aminomalonate


organism: Acinetobacter radioresistens

proof_lines: mutant K18A/V287I, pH not specified in the publication temperature not specified in the publication


smiles: CC(C(C(=O)O)N)C(=O)O


proof_lines: wild-type, pH not specified in the publication temperature not specified in the publication


substrate_name: L-alanine

smiles: CC(C(=O)O)N

substrate: L-alanine


organism: Lactiplantibacillus plantarum subsp. plantarum


substrate_name: L-Glutamate; 4-Aminobutanoate; CO2

substrate: L-Glutamate; 4-Aminobutanoate; CO2


substrate_name: L-Glutamate; CO2; 4-Aminobutanoate

substrate: L-Glutamate; CO2; 4-Aminobutanoate


organism: Aedes aegypti


proof_lines: wild-type, 23°C, pH not specified in the publication


proof_lines: mutant E88R, 23°C, pH not specified in the publication


organism: Pseudomonas dacunhae

proof_lines: in presence of 2-oxoglutarate or pyridoxal 5'-phosphate


proof_lines: mutant enzyme R37A, in 50 mM potassium phosphate, at pH 7.0 and 37°C


proof_lines: wild type enzyme, in 50 mM potassium phosphate, at pH 7.0 and 37°C


organism: Cunninghamella elegans


assay_conditions_summary: pH 7; 298.15 K; buffer 0.2 M phosphate

proof_lines: in 0.2 M phosphate buffer (pH 7.0) containing 0.1 mM pyridoxal 5'-phosphate, at 25°C


organism: Mycobacterium tuberculosis


organism: Penicillium citrinum


organism: Rattus norvegicus

proof_lines:
brain enzyme
liver and kidney enzyme


enzyme_display_name: malonyl-CoA decarboxylase

organism: Aix sponsa

substrate_name: malonyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: malonyl-CoA decarboxylase

substrate: malonyl-CoA


organism: Anser domesticus


organism: Branta leucopsis


organism: Anopheles gambiae

assay_conditions_summary: pH 7; buffer 0.2 M phosphate


organism: Saccharopolyspora erythraea

substrate_name: N-hydroxy-L-ornithine

proof_lines: in 20 mM Tris, 100 mM NaCl, pH 7.2, at 25°C

substrate: N-hydroxy-L-ornithine


organism: Corynebacterium jeikeium

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature A74G to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 7


sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature E42Q to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature E42D to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature R3K to reconstruct the assayed variant sequence.


organism: Homo sapiens

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature E302G with inferred residue-numbering offset +12 to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 8.5; cofactor CoA

proof_lines: pH 8.5, temperature not specified in the publication, mutant E302G, no H2O2


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature C243S with inferred residue-numbering offset -6 to reconstruct the assayed variant sequence.

proof_lines: pH 8.5, temperature not specified in the publication, mutant C243S, no H2O2

proof_lines: pH 8.5, temperature not specified in the publication, mutant C243S, 0.1 M H2O2

proof_lines: pH 8.5, temperature not specified in the publication, mutant C243S, 0.2 M H2O2


proof_lines: pH 8.5, temperature not specified in the publication, wild-type enzyme, 0.2 M H2O2

proof_lines: pH 8.5, temperature not specified in the publication, wild-type enzyme, 0.05 M H2O2

proof_lines: pH 8.5, temperature not specified in the publication, wild-type enzyme, 0.1 M H2O2


proof_lines: pH 8.5, temperature not specified in the publication, mutant C206S, 0.2 M H2O2

proof_lines: pH 8.5, temperature not specified in the publication, mutant C206S, no H2O2

proof_lines: pH 8.5, temperature not specified in the publication, mutant C206S, 00.1 M H2O2


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature C206S/C243S to reconstruct the assayed variant sequence.

proof_lines: pH 8.5, temperature not specified in the publication, mutant C206S/C243S, no H2O2

proof_lines: pH 8.5, temperature not specified in the publication, mutant C206S/C243S, 0.2 M H2O2

proof_lines: pH 8.5, temperature not specified in the publication, mutant C206S/C243S, 0.1 M H2O2


organism: Mus musculus

proof_lines: kcat is 3.6 sec(-1) with 3-sulfino-L-alanine (cysteine sulfinic acid) as substrate. kcat 1.3 sec(-1) is with L-aspartate as substrate.


proof_lines: kcat is 6.6 sec(-1) with 3-sulfino-L-alanine (cysteine sulfinic acid) as substrate. kcat is 0.14 sec(-1) with L-aspartate as substrate.


organism: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)

verification_status: verified

assay_conditions_summary: pH 8

column_label: Table 1 row L-aspartate column Km (mM) has cell 1.2 +/- 0.1 mM.

raw_value_unit_evidence: Km raw_value=1.2 +/- 0.1 raw_unit=mM

row_label: L-aspartate

table_label: Table 1. Substrate Specificity of PhGAD from P. horikoshii

status: verified


column_label: Table 1 row L-glutamate column Km (mM) has cell 3.9 +/- 0.4 mM.

raw_value_unit_evidence: Km raw_value=3.9 +/- 0.4 raw_unit=mM

row_label: L-glutamate


substrate_name: cysteate

column_label: Table 1 row Cysteate column Km (mM) has cell 2.2 +/- 0.3 mM.

raw_value_unit_evidence: Km raw_value=2.2 +/- 0.3 raw_unit=mM

row_label: Cysteate

substrate: cysteate


organism: Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)

substrate_name: aspartate

proof_lines: kcat is 0.80 sec(-1) with aspartate as substrate. kcat is 0.18 sec(-1) with glutamate as substrate.

substrate: aspartate


substrate_name: glutamate

substrate: glutamate


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

proof_lines: kcat is 0.65 sec(-1), specific activity 2100 nmol/min/mg for L-aspartate.


organism: Pseudomonas sp.

source_databases_merged: brenda uniprot


organism: Aliivibrio fischeri (strain ATCC 700601 / ES114)

substrate_name: aspartate (at 28 degrees Celsius)

proof_lines: kcat is 0.075 sec(-1) at 28 degrees Celsius. kcat is 0.008 sec(-1) at 37 degrees Celsius.

substrate: aspartate (at 28 degrees Celsius)


substrate_name: glutamate (at 37 degrees Celsius)

proof_lines: kcat is 0.055 sec(-1) at 37 degrees Celsius.

substrate: glutamate (at 37 degrees Celsius)


substrate_name: Malonyl-CoA

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

sequence_source: multiple

proof_lines: pH and temperature not specified in the publication

proof_lines: kcat is 19 sec(-1) with malonyl-CoA for malonyl-CoA decarboxylase mitochondrial form (PubMed:15003260). kcat is 28 sec(-1) with malonyl-CoA for Malonyl-CoA decarboxylase cytoplasmic+peroxisomal form (PubMed:15003260). The catalytic efficien

source_databases_merged: sabio_rk brenda skid uniprot

substrate: Malonyl-CoA


mutation_signature: full-length and N-terminal truncated apo-hMCD full-length and N-terminal truncated apo-hMCD

sequence_variant_note: Mutation context was captured, but the signature could not be translated into an exact residue substitution safely.


organism: Brucella microti

assay_conditions_summary: pH 4.6; 298.15 K; buffer 50 mM sodium acetate


substrate_name: L-Glu

proof_lines: GAD65 after cleavage with protease factor Xa

proof_lines: GAD65-GST fusion protein expressed in Escherichia coli

substrate: L-Glu


organism: Latilactobacillus sakei

substrate_name: L-glutamate

verification_status: corrected

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

column_label: PubMed abstract reports: The Km and Vmax of GAD were 0.045 mM and 0.011 mM/min, respectively, when glutamate was used as the substrate.

raw_value_unit_evidence: Km raw_value=0.045 mM raw_unit=mM

row_label: Purified Lactobacillus sakei GAD / glutamate

table_label: PubMed XML abstract PMID 25791853

substrate: L-glutamate

status: corrected


organism: Levilactobacillus senmaizukei

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Bos taurus

proof_lines:
in presence of 150 mM PO43-
in presence of 40 mM PO43-


organism: Rattus norvegicus

sequence: MRGLGPSLRARRLLPLRYPPR

wild_type_sequence: MRGLGPSLRARRLLPLRYPPR

assay_conditions_summary: pH 7; cofactor CoA

column_label: Abstract reports recombinant rat malonyl-CoA decarboxylase showed Michaelis-Menten saturation and Km was calculated to be 68 microM.

raw_value_unit_evidence: Km raw_value=68 microM raw_unit=microM

row_label: Rat malonyl-CoA decarboxylase / malonyl-CoA

table_label: PubMed XML abstract PMID 12297032


organism: Synechocystis sp. PCC 6803


organism: Levilactobacillus brevis

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature K17T/D294G/E312S/Q346H to reconstruct the assayed variant sequence.

proof_lines: C-terminal truncation mutant K17T/D294G/E312S/Q346H, pH not specified in the publication, temperature not specified in the publication


substrate_name: cysteine sulfite

column_label: Table 1 row Cysteine sulfite column Km (mM) has cell 32.6 +/- 5.4 mM.

raw_value_unit_evidence: Km raw_value=32.6 +/- 5.4 raw_unit=mM

row_label: Cysteine sulfite

substrate: cysteine sulfite


substrate_name: Unknown

substrate: Unknown


proof_lines: kcat is 0.34 sec(-1) with L-aspartate as substrate. kcat is 0.26 sec(-1) with L-glutamate as substrate. kcat is 0.65 sec(-1) with cysteate as substrate. kcat is 0.03 sec(-1) with cysteine sulfite as substrate.


proof_lines: pH 7.3, 25°C, GAD65, recombinant enzyme expressed in Escherichia coli


organism: Bacillus subtilis

substrate_name: L-Aspartate

sequence_variant_note: Mutation signature I188M references residue 188, which is outside the resolved sequence length.

proof_lines:
L-aspartate-α-decarboxylase
specific activity
thermostability

substrate: L-Aspartate


sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


substrate_name: 3-sulfino-L-alanine (cysteine sulfinic acid)


organism: Escherichia coli

proof_lines:
aspartate alpha-decarboxylase
Escherichia coli
L-aspartate

source_databases_merged: sabio_rk brenda uniprot


enzyme_display_name: ornithine decarboxylase

organism: Lactobacillus sp. 30a

substrate_name: alpha-Methylornithine

smiles: CC(CCCN)(C(=O)O)N

enzyme: ornithine decarboxylase

substrate: alpha-Methylornithine


enzyme_display_name: Lysine/ornithine decarboxylase

organism: Selenomonas ruminantium

substrate_name: L-lysine

smiles: C(CCN)CC(C(=O)O)N

enzyme: Lysine/ornithine decarboxylase

substrate: L-lysine


enzyme_display_name: UniProt P0A9H3

proof_lines:
Raw SKiD Env: Temp=10°C, pH=6.5
Raw SKiD Env: Temp=45°C, pH=5.6

enzyme: UniProt P0A9H3


substrate_name: L-ornithine

smiles: C(CC(C(=O)O)N)CN

substrate: L-ornithine


enzyme_display_name: UniProt P9WIU7

organism: Mycobacterium tuberculosis

smiles: C(CC(C(=O)O)N)CC(C(=O)O)N

proof_lines:
Raw SKiD Env: Temp=-----, pH=7.8
Raw SKiD Env: Temp=25°C, pH=7.8
Raw SKiD Env: Temp=37°C, pH=8

enzyme: UniProt P9WIU7


enzyme_display_name: UniProt Q73PV9

organism: Treponema denticola

proof_lines:
Raw SKiD Env: Temp=30°C, pH=8
Raw SKiD Env: Temp=10°C, pH=8

enzyme: UniProt Q73PV9


substrate_name: 5-aminoimidazole ribonucleotide

substrate: 5-aminoimidazole ribonucleotide


enzyme_display_name: histidine decarboxylase

organism: Staphylococcus epidermidis

substrate_name: L-histidine

enzyme: histidine decarboxylase

substrate: L-histidine


enzyme_display_name: UniProt O26232

organism: Methanothermobacter thermautotrophicus

substrate_name: orotidine 5'-phosphate

proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=25°C, pH=7.1

enzyme: UniProt O26232

substrate: orotidine 5'-phosphate


enzyme_display_name: UniProt Q8IJH3

organism: Plasmodium falciparum

enzyme: UniProt Q8IJH3


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature H465A with inferred residue-numbering offset -14 to reconstruct the assayed variant sequence.


substrate_name: L-Glutamate

substrate: L-Glutamate


organism: Methanocaldococcus jannaschii

substrate_name: L-aspartate

source_license: CC BY 4.0; CC BY-NC-ND 4.0

source_databases_merged: skid uniprot

substrate: L-aspartate


organism: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

substrate_name: L-tyrosine

column_label: The ScienceDirect full text states that the enzyme had a specific activity for the decarboxylation of L-tyrosine of 1.1 (+/- 0.12) U mg-1 protein with a Km of 1.6 (+/- 0.29) mM.

raw_value_unit_evidence: Km raw_value=1.6 raw_unit=mM

row_label: Methanocaldococcus jannaschii MfnA / L-tyrosine

table_label: ScienceDirect full text PMID 15715981 MfnA enzyme characterization prose

substrate: L-tyrosine


organism: Brucella microti


organism: Achromobacter sp.

substrate_name: L-Asp

proof_lines:
l-aspartate 4-carboxy-lyase
Achromobacter sp.

substrate: L-Asp


organism: Homo sapiens


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature Q5D/V6I/T7E to reconstruct the assayed variant sequence.


organism: Lacticaseibacillus paracasei

column_label: Abstract reports Km value for glutamate was 5.0 mM.

raw_value_unit_evidence: Km raw_value=5.0 mM raw_unit=mM

row_label: Lactobacillus paracasei NFRI 7415 GAD / L-glutamate

table_label: PubMed XML abstract PMID 18256502


organism: Rattus norvegicus

substrate_name: Malonyl-CoA


organism: Comamonas testosteroni

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


organism: Synechocystis sp. PCC 6803

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

column_label: PubMed abstract for PMID 26730883 reports catalytic properties for the enzyme: Km 19.6 mM, kcat 100.7 s(-1), and kcat/Km 5.1 mM(-1) s(-1).

normalized_value_unit_evidence: Km normalized_value=19.6 normalized_unit=mM; kcat normalized_value=100.7 normalized_unit=s^-1; kcat/Km paper rounded normalized_value=5.1 normalized_unit=s^-1*mM^-1; exported central ratio 100.7/19.6=5.137755102040816 s^-1*mM^-1.

raw_value_unit_evidence: Km raw_value=19.6 raw_unit=mM; kcat raw_value=100.7 raw_unit=s^-1; kcat/Km printed raw_value=5.1 raw_unit=mM^-1 s^-1.

row_label: Synechocystis sp. PCC6803 glutamate decarboxylase / L-glutamate

table_label: PubMed XML abstract PMID 26730883


organism: Cairina moschata

substrate_name: malonyl-CoA

assay_conditions_summary: pH 8; cofactor CoA

column_label: PMID 102255 DOI 10.1016/0003-9861(78)90314-4 Table I; row Muscovy duck; column Malonyl-CoA Km (uM).

raw_value_unit_evidence: Table I row Muscovy duck: Malonyl-CoA Km 32 uM; supporting prose gives 32 uM and V 75 umol/min/mg for the muscovy duck enzyme.

row_label: Muscovy duck (Cairina moschatta / Cairina moschata)

table_label: Table I. Malonyl-CoA decarboxylase content and fatty acid composition of the uropygial glands of geese and ducks and Michaelis-Menten constants of the enzymes from these glands

substrate: malonyl-CoA


organism: Levilactobacillus brevis

substrate_name: L-glutamate


proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


organism: Bacillus subtilis


proof_lines: pH 7.0, 37°C, recombinant wild-type enzyme


organism: Escherichia coli

assay_conditions_summary: pH 4.6; 310.15 K; buffer 0.2 M pyridine/HCl

proof_lines: mutant enzyme DELTAHT, in 0.2 M pyridine/HCl buffer, pH 4.6, at 37°C


proof_lines: mutant enzyme H465A, in 0.2 M pyridine/HCl buffer, pH 4.6, at 37°C


proof_lines: wild type enzyme, in 0.2 M pyridine/HCl buffer, pH 4.6, at 37°C

proof_lines: pH 4.6, 37°C, recombinant C-terminally His-tagged wild-type enzyme

proof_lines: pH 4.6, 37°C, recombinant untagged wild-type enzyme


organism: Bos taurus

substrate_name: L-Glu

proof_lines: from pH 5.8 to pH 7.8 the Km decreases from 1.7 mM at pH 5.8 to 0.5 mM at pH 7.8


substrate_name: cysteine sulfinic acid

substrate: cysteine sulfinic acid


substrate_name: L-cysteic acid

substrate: L-cysteic acid


organism: Priestia megaterium

proof_lines: recombinant wild-type enzyme, pH 5.0, 50°C

substrate: L-glutamate


proof_lines: mutant with N-terminal deletion of 90 amino acids, pH 7.2


proof_lines: mutant with N-terminal deletion of 70 amino acids, pH 7.2


organism: Lactiplantibacillus plantarum subsp. plantarum

proof_lines: pH 5.0, 37°C, recombinant wild-type enzyme


proof_lines: pH 7.0, 37°C, recombinant enzyme mutant DELTA11

proof_lines: pH 5.0, 37°C, recombinant enzyme mutant DELTA11


organism: Lactiplantibacillus plantarum

assay_conditions_summary: pH 5

proof_lines: wild-type, pH 5, temperature not specified in the publication


mutation_signature: C-terminal trunction mutant

proof_lines: C-terminal trunction mutant, pH 5, temperature not specified in the publication


proof_lines: pH 4.6, 37°C, recombinant deletion mutant GadBDELTA1-14


proof_lines: pH 4.6, 37°C, recombinant mutant Q5D/V6I/T7E


organism: Priestia aryabhattai


organism: Tribolium castaneum

proof_lines:
wild-type, pH 7, 37°C
wild-type, pH 6.5, 37°C


organism: Alcaligenes sp.


organism: Anas platyrhynchos


organism: Anser sp.


organism: Aspergillus oryzae

substrate: L-Glu


organism: Bacillus

proof_lines:
isoform GadZ20, pH 5.0, 40°C
isoform GadZ1, pH 5.0, 40°C
isoform GadZ11, pH 5.0, 40°C


organism: Camellia sinensis


organism: Clostridium perfringens


organism: Ctenocephalides felis


organism: Cucurbita maxima


organism: Drosophila melanogaster


organism: Enterococcus avium

proof_lines: pH 5.5, 45°C, recombinant His-tagged enzyme


organism: Enterococcus faecium


organism: Enterococcus raffinosus


organism: Escherichia coli


substrate_name: L-alpha-Methylglutamate

substrate: L-alpha-Methylglutamate


organism: Felis catus


organism: Homo sapiens

proof_lines: GAD67 after cleavage with protease factor Xa

proof_lines: GAD67-GST fusion protein expressed in Escherichia coli

proof_lines: isoform GAD67, lacking aminoterminal 70 amino acids, presence of apocalmodulin


substrate_name: Unknown

proof_lines: pH 7.3, 25°C, GAD67, recombinant enzyme expressed in Escherichia coli

proof_lines: pH 7.3, 25°C, GAD56, recombinant enzyme expressed in Escherichia coli

substrate: Unknown


enzyme_display_name: UniProt Q8RY79

organism: Arabidopsis thaliana

substrate_name: L-phenylalanine

enzyme: UniProt Q8RY79

substrate: L-phenylalanine


enzyme_display_name: tyrosine decarboxylase

organism: Pyrococcus furiosus

enzyme: tyrosine decarboxylase


enzyme_display_name: UniProt Q88JU5

organism: Pseudomonas putida

substrate_name: L-Dopa

enzyme: UniProt Q88JU5

substrate: L-Dopa


enzyme_display_name: UniProt P00864

substrate_name: phosphoenolpyruvate

enzyme: UniProt P00864

substrate: phosphoenolpyruvate


enzyme_display_name: Phosphoenolpyruvate carboxylase

organism: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)

enzyme: Phosphoenolpyruvate carboxylase


enzyme_display_name: UniProt P21642

organism: Gallus gallus

substrate_name: GDP

enzyme: UniProt P21642

substrate: GDP


enzyme_display_name: UniProt P35558

enzyme: UniProt P35558


enzyme_display_name: glutamate decarboxylase

organism: Hordeum vulgare

enzyme: glutamate decarboxylase


organism: Levilactobacillus brevis

assay_conditions_summary: pH 5.2; 318.15 K; buffer acetate

proof_lines:
pH 5.2, 45°C, recombinant enzyme
pH 4.6, 37°C, native enzyme
pH 4.6, 37°C, recombinant enzyme


proof_lines: C-terminal truncation mutant lacking 11 amino acids, pH not specified in the publication, temperature not specified in the publication


enzyme_display_name: aspartate 1-decarboxylase

organism: Methanocaldococcus jannaschii

enzyme: aspartate 1-decarboxylase


organism: Mus musculus


organism: Monascus purpureus


organism: Mycobacterium tuberculosis