substrate_name: O2; H+; Androst-4-ene-3,17-dione; NADPH

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R264C to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aromatase(Enzyme) mutant R264C His-tagged

substrate: O2; H+; Androst-4-ene-3,17-dione; NADPH


enzyme_display_name: aromatase

organism: Homo sapiens

substrate_name: H+; O2; NADPH; Testosterone

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

protein_accession_database: UniProt

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

km_unit: mM

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature C299A to reconstruct the assayed variant sequence.

mutation_type: substitution

assay_conditions_summary: cofactor NADPH

proof_lines: SABIO-RK modifier species: aromatase(Enzyme) mutant C299A

enzyme: aromatase

substrate: H+; O2; NADPH; Testosterone


substrate_name: O2; H+; NADPH; Androst-4-ene-3,17-dione

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R264H to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aromatase(Enzyme) mutant R264H His-tagged

substrate: O2; H+; NADPH; Androst-4-ene-3,17-dione


enzyme_display_name: psoralen synthase

organism: Ammi majus

substrate_name: Marmesin

source_db: oed

source_license: CC BY 4.0

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

enzyme: psoralen synthase

substrate: Marmesin

source: oed


substrate_name: marmesin

source_db: uniprot

mutation_signature: Unknown

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

substrate: marmesin

source: uniprot


enzyme_display_name: abieta-7,13-dien-18-ol hydroxylase

organism: Pinus taeda

substrate_name: H+; NADPH; O2; Abietadienol

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

enzyme: abieta-7,13-dien-18-ol hydroxylase

substrate: H+; NADPH; O2; Abietadienol


organism: Pastinaca sativa


organism: Kitagawia praeruptora

substrate_name: (+)-marmesin

source_db: brenda

substrate: (+)-marmesin

source: brenda


substrate_name: abietadienol

substrate: abietadienol


organism: Apium graveolens


enzyme_display_name: steroid 21-monooxygenase

organism: Bos taurus

km_display: Not reported

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

enzyme: steroid 21-monooxygenase


organism: Sus scrofa


enzyme_display_name: squalene monooxygenase

organism: Candida albicans

substrate_name: squalene

enzyme: squalene monooxygenase

substrate: squalene


organism: Candida parapsilosis


organism: Saccharomyces cerevisiae


enzyme_display_name: taxadiene 5alpha-hydroxylase

organism: Taxus cuspidata

substrate_name: taxa-4(5),11(12)-diene (for the native enzyme)

enzyme: taxadiene 5alpha-hydroxylase


enzyme_display_name: steroid 22S-hydroxylase

organism: Arabidopsis thaliana

substrate_name: campesterol

enzyme: steroid 22S-hydroxylase

substrate: campesterol


enzyme_display_name: heme oxygenase (biliverdin-producing)

substrate_name: Heme; NADPH; O2

sequence_source: UniProtKB reviewed accession Q5E9F2, checked via https://rest.uniprot.org/uniprotkb/Q5E9F2.json

enzyme: heme oxygenase (biliverdin-producing)

substrate: Heme; NADPH; O2


enzyme_display_name: steroid 17alpha-monooxygenase

organism: Danio rerio

substrate_name: progesterone

enzyme: steroid 17alpha-monooxygenase

substrate: progesterone


organism: Rattus norvegicus

substrate_name: Hematin; O2; NADPH

data_origin: experimental

substrate: Hematin; O2; NADPH


organism: Mus musculus

substrate_name: progesterone (17-alpha hydroxylation)

substrate: progesterone (17-alpha hydroxylation)


assay_conditions_summary: pH 7.4; 310.15 K; cofactor NADPH


enzyme_display_name: 17alpha-hydroxyprogesterone deacetylase

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature N202S to reconstruct the assayed variant sequence.

enzyme: 17alpha-hydroxyprogesterone deacetylase


sequence_source: source_record; UniProt P27786 direct check


organism: Sorghum bicolor

substrate_name: p-hydroxyphenylacetaldoxime

substrate: p-hydroxyphenylacetaldoxime


enzyme_display_name: valine N-monooxygenase

organism: Lotus japonicus

substrate_name: L-valine

kcat_over_km_origin: calculated

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

smiles: CC(C)C(C(=O)O)N

enzyme: valine N-monooxygenase

substrate: L-valine


organism: Manihot esculenta

substrate_name: H+; O2; NADPH; L-Valine

assay_conditions_summary: pH 7.9; 303.15 K; cofactor NADP+

substrate: H+; O2; NADPH; L-Valine


organism: Homo sapiens


enzyme_display_name: isoleucine N-monooxygenase

substrate_name: L-isoleucine

smiles: CCC(C)C(C(=O)O)N

enzyme: isoleucine N-monooxygenase

substrate: L-isoleucine


enzyme_display_name: phenylalanine N-monooxygenase

substrate_name: phenylalanine

enzyme: phenylalanine N-monooxygenase

substrate: phenylalanine


enzyme_display_name: (E)-2-methylbutanal oxime monooxygenase

substrate_name: (E)-2-methylbutanal oxime

enzyme: (E)-2-methylbutanal oxime monooxygenase

substrate: (E)-2-methylbutanal oxime


enzyme_display_name: (R)-limonene 6-monooxygenase

enzyme: (R)-limonene 6-monooxygenase


enzyme_display_name: (S)-limonene 7-monooxygenase

smiles: CCC=CCC=CCC=CCC=CCC=CCCCC(=O)O

enzyme: (S)-limonene 7-monooxygenase


enzyme_display_name: quinine 3-monooxygenase

substrate_name: Quinine

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature P467S to reconstruct the assayed variant sequence.

proof_lines: PMCID PMC6555127 Table 2 row CYP3A4*19 (P467S).

enzyme: quinine 3-monooxygenase

substrate: Quinine


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature M445T to reconstruct the assayed variant sequence.

proof_lines: PMCID PMC6555127 Table 2 row CYP3A4*3 (M445T).


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature Q200H to reconstruct the assayed variant sequence.

proof_lines: PMCID PMC6555127 Table 2 row CYP3A4*24 (Q200H).


sequence_variant_note: Resolved canonical sequence from Untrusted worker-applied substitution from canonical UniProt P08684; no trusted row-specific CYP3A4*17 sequence preserved. and applied mutation signature F189S to reconstruct the assayed variant sequence.

proof_lines: PMCID PMC6555127 Table 2 row CYP3A4*17 (F189S).


sequence_variant_note: Resolved canonical sequence from UniProt REST P08684 canonical sequence with worker-applied substitution from selected BRENDA/PMID variant label and applied mutation signature I427V to reconstruct the assayed variant sequence.

proof_lines: PMCID PMC6555127 Table 2 row CYP3A4*34 (I427V).


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature T363M to reconstruct the assayed variant sequence.

proof_lines: PMCID PMC6555127 Table 2 row CYP3A4*11 (T363M).


enzyme_display_name: 1,8-cineole 2-exo-monooxygenase

substrate_name: 17beta-estradiol (2-hydroxylation)

enzyme: 1,8-cineole 2-exo-monooxygenase

substrate: 17beta-estradiol (2-hydroxylation)


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature L293P to reconstruct the assayed variant sequence.

proof_lines: PMCID PMC6555127 Table 2 row CYP3A4*18 (L293P).


substrate_name: taurochenodeoxycholate

substrate: taurochenodeoxycholate


enzyme_display_name: trimethyltridecatetraene synthase

substrate_name: (E,E)-geranyllinalool

enzyme: trimethyltridecatetraene synthase

substrate: (E,E)-geranyllinalool


organism: Zea mays

substrate_name: NADPH; O2; (6E,10E)-Geranyllinalool; H+

substrate: NADPH; O2; (6E,10E)-Geranyllinalool; H+


substrate_name: (E)-nerolidol

substrate: (E)-nerolidol


enzyme_display_name: dimethylnonatriene synthase

enzyme: dimethylnonatriene synthase


enzyme_display_name: syn-pimaradiene 3-monooxygenase

organism: Oryza sativa

enzyme: syn-pimaradiene 3-monooxygenase


enzyme_display_name: long-chain fatty acid omega-monooxygenase

organism: Starmerella bombicola

substrate_name: oleic acid

substrate: oleic acid


enzyme_display_name: flavonoid 3',5'-hydroxylase

organism: Billardiera heterophylla

substrate_name: dihydrokaempferol

enzyme: flavonoid 3',5'-hydroxylase

substrate: dihydrokaempferol


substrate_name: kaempferol

substrate: kaempferol


substrate_name: naringenin

substrate: naringenin


enzyme_display_name: flavonoid 3'-monooxygenase

organism: Perilla frutescens

enzyme: flavonoid 3'-monooxygenase


organism: Fragaria vesca


enzyme_display_name: geraniol 8-hydroxylase

organism: Catharanthus roseus

substrate_name: nerol

enzyme: geraniol 8-hydroxylase

substrate: nerol


enzyme_display_name: pentalenic acid synthase

organism: Streptomyces avermitilis

substrate_name: Compactin

source_license: CC BY 4.0; CC BY-NC-ND 4.0

proof_lines:
pH 7.4, 30°C
pH 8.0, 25°C
Raw SKiD Env: Temp=30°C, pH=7.4

source_databases_merged: brenda skid

enzyme: pentalenic acid synthase

substrate: Compactin


enzyme_display_name: cholestanetriol 26-monooxygenase

substrate_name: O2; 3alpha,7alpha,12alpha-Trihydroxy-5beta-cholestane; H+; NADPH

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

enzyme: cholestanetriol 26-monooxygenase


enzyme_display_name: vitamin D3 24-hydroxylase

organism: Gallus gallus

substrate_name: Calcidiol; NADPH; H+; O2

verification_status: corrected

km_unit: µM

sequence_source: Selected-batch canonical sequence from UniProt REST O73684

enzyme: vitamin D3 24-hydroxylase

substrate: Calcidiol; NADPH; H+; O2

status: corrected


enzyme_display_name: calcidiol 1-monooxygenase

substrate_name: 25-hydroxyvitamin D3

enzyme: calcidiol 1-monooxygenase

substrate: 25-hydroxyvitamin D3


substrate_name: O2; Calcidiol; H+; NADPH

substrate: O2; Calcidiol; H+; NADPH


enzyme_display_name: zeaxanthin epoxidase

organism: Spinacia oleracea

substrate_name: NADH; H+; O2; Zeaxanthin

assay_conditions_summary: cofactor NADH

enzyme: zeaxanthin epoxidase

substrate: NADH; H+; O2; Zeaxanthin


organism: Sus scrofa

substrate_name: H+; Calcidiol; O2; NADPH

substrate: H+; Calcidiol; O2; NADPH


organism: Mycolicibacterium smegmatis


enzyme_display_name: alkane 1-monooxygenase

organism: Ectopseudomonas oleovorans

substrate_name: laurate

enzyme: alkane 1-monooxygenase

substrate: laurate


organism: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)


enzyme_display_name: corticosterone 18-monooxygenase

substrate_name: cortisol

enzyme: corticosterone 18-monooxygenase

substrate: cortisol


enzyme_display_name: steroid 15beta-monooxygenase

organism: Priestia megaterium

substrate_name: deoxycorticosterone

enzyme: steroid 15beta-monooxygenase

substrate: deoxycorticosterone


enzyme_display_name: phenylalanine 4-monooxygenase

organism: Chromobacterium violaceum

substrate_name: L-Phenylalanine

sequence_source: UniProtKB P30967 reviewed-entry check on 2026-08-25; canonical accession context only, not a validated S230P variant sequence.

enzyme: phenylalanine 4-monooxygenase

substrate: L-Phenylalanine


substrate_name: L-cyclohexylalanine

substrate: L-cyclohexylalanine


organism: Caenorhabditis elegans

substrate_name: tetrahydrobiopterin

verification_status: unverified

sequence_source: UniProtKB REST Q9XYQ5 -> primary P90925 (checked 2026-06-30)

assay_conditions_summary: pH 4

substrate: tetrahydrobiopterin

status: unverified


organism: Homo sapiens


enzyme_display_name: tyrosine 3-monooxygenase

enzyme: tyrosine 3-monooxygenase


organism: Rattus norvegicus


enzyme_display_name: tryptophan 5-monooxygenase

organism: Dictyostelium discoideum

substrate_name: L-Phe with BH(4) as cofactor

enzyme: tryptophan 5-monooxygenase

substrate: L-Phe with BH(4) as cofactor


substrate_name: L-phenylalanine (at 25 degrees Celsius)

substrate: L-phenylalanine (at 25 degrees Celsius)


enzyme_display_name: dopamine beta-monooxygenase

substrate_name: ascorbate

enzyme: dopamine beta-monooxygenase

substrate: ascorbate


enzyme_display_name: aminocyclopropanecarboxylate oxidase

organism: Malus domestica

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S246A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant S246A

proof_lines: recombinant His-tagged mutant S246A, pH 7.2, 20°C

enzyme: aminocyclopropanecarboxylate oxidase


organism: Diospyros kaki


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature E109M to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant E109M


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S257A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant S257A


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature V159G to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant V159G


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S246F to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant S246F


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S246G to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant S246G


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S246Y to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant S246Y


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature T157A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant T157A

proof_lines: recombinant His-tagged mutant T157A, pH 7.2, 20°C


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R244K to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant R244K

proof_lines: recombinant His-tagged mutant R244K, pH 7.2, 20°C


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R244A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant R244A


sequence_variant_note: Resolved canonical sequence from Not accepted for this variant: the supplied provenance is a reconstruction from canonical UniProtKB Q00985, not trusted R244G row-specific sequence evidence. and applied mutation signature R244G to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant R244G


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R175Q to reconstruct the assayed variant sequence.

proof_lines: recombinant His-tagged mutant R175Q, pH 7.2, 20°C


enzyme_display_name: tyrosinase

organism: Bos taurus

substrate_name: L-Adrenaline; O2

sequence_source: UniProtKB exact accession Q8MIU0 REST lookup

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

enzyme: tyrosinase

substrate: L-Adrenaline; O2


substrate_name: O2; L-Adrenaline

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

substrate: O2; L-Adrenaline


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S246T to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant S246T


organism: Euterpe edulis

substrate_name: chlorogenic acid

substrate: chlorogenic acid


organism: Camellia sinensis

substrate_name: catechol

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

substrate: catechol


organism: Neurospora crassa

substrate_name: L-Dopa

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

substrate: L-Dopa


organism: Euterpe oleracea

sequence_source: UniProtKB direct accession lookup: Q3YJ63


organism: Priestia aryabhattai

substrate_name: L-tyrosine

substrate: L-tyrosine


organism: Triticum aestivum

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


organism: Carthamus tinctorius

smiles: O=O


enzyme_display_name: sphingolipid 4-desaturase

organism: Gallus gallus

substrate_name: all-trans-retinol

enzyme: sphingolipid 4-desaturase

substrate: all-trans-retinol


organism: Pholiota microspora

substrate_name: L-dopa

substrate: L-dopa


enzyme_display_name: acyl-CoA 6-desaturase

substrate_name: linoleoyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: acyl-CoA 6-desaturase

substrate: linoleoyl-CoA


enzyme_display_name: (S)-nandinine synthase

organism: Eschscholzia californica

substrate_name: H+; NADPH; O2; (R,S)-Scoulerine

enzyme: (S)-nandinine synthase

substrate: H+; NADPH; O2; (R,S)-Scoulerine


enzyme_display_name: acyl-lipid omega-3 desaturase (cytochrome b5)

organism: Mortierella alpina

substrate_name: alpha-linolenoyl-CoA

sequence_source: UniProtKB U5Y6A8 (canonical sequence available; direct accession lookup)

assay_conditions_summary: pH 7.5; 301.15 K; cofactor CoA

substrate: alpha-linolenoyl-CoA


enzyme_display_name: flavone synthase II

organism: Glycine max

substrate_name: liquiritigenin

enzyme: flavone synthase II

substrate: liquiritigenin


substrate_name: eriodictyol

substrate: eriodictyol


organism: Oryza sativa subsp. japonica

substrate_name: naringenin

substrate: naringenin


organism: Dictyostelium discoideum

substrate_name: L-Phe with BH(4) as cofactor

substrate: L-Phe with BH(4) as cofactor


enzyme_display_name: spheroidene monooxygenase

organism: Rubrivivax gelatinosus

substrate_name: spheroidene

enzyme: spheroidene monooxygenase

substrate: spheroidene


enzyme_display_name: 6beta-hydroxyhyoscyamine epoxidase

organism: Hyoscyamus niger

enzyme: 6beta-hydroxyhyoscyamine epoxidase


organism: Xenorhabdus nematophila


enzyme_display_name: anthocyanidin synthase

organism: Perilla frutescens

enzyme: anthocyanidin synthase


enzyme_display_name: flavone synthase I

organism: Plagiochasma appendiculatum

enzyme: flavone synthase I


enzyme_display_name: flavonol synthase

organism: Camellia sinensis

substrate_name: hesperetin

sequence_source: UniProtKB:A0A3G1RNB1 direct exact-accession lookup

enzyme: flavonol synthase

substrate: hesperetin


organism: Crocosmia x crocosmiiflora

sequence_source: Direct UniProtKB JSON/FASTA lookup for A0A4D6Q440


organism: Pseudomonas syringae

smiles: O=C(O)CCC(=O)C(=O)O


enzyme_display_name: prostaglandin-endoperoxide synthase

organism: Mus musculus

substrate_name: arachidonate

smiles: CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(=O)O

enzyme: prostaglandin-endoperoxide synthase

substrate: arachidonate


enzyme_display_name: tyrosine isonitrile desaturase

organism: Pseudomonas aeruginosa

enzyme: tyrosine isonitrile desaturase


organism: Ovis aries

smiles: CCCCCC(C=CC=CCC=CCC=CCCCC(=O)O)OO


smiles: OO


enzyme_display_name: Deleted entry

organism: Rattus norvegicus

substrate_name: H+; NADPH; Aquacob(III)alamin

enzyme: Deleted entry

substrate: H+; NADPH; Aquacob(III)alamin


organism: Euglena gracilis

substrate_name: H+; NADPH; Hydroxycob(lll)alamin

substrate: H+; NADPH; Hydroxycob(lll)alamin


enzyme_display_name: ferric-chelate reductase (NADH)

organism: Solanum lycopersicum

substrate_name: Fe3+; NADH; H+

enzyme: ferric-chelate reductase (NADH)

substrate: Fe3+; NADH; H+


organism: Paracoccus denitrificans

substrate_name: NADH; Fe3+; H+

substrate: NADH; Fe3+; H+


enzyme_display_name: ferric-chelate reductase (NADPH)

organism: Homo sapiens

substrate_name: NADPH; Fe3+

sequence_source: UniProtKB P30043 canonical entry (accession-backed compact identity evidence)

enzyme: ferric-chelate reductase (NADPH)

substrate: NADPH; Fe3+


enzyme_display_name: xanthine dehydrogenase

organism: Capra hircus

substrate_name: xanthine

assay_conditions_summary: pH 7.2; 298.15 K; cofactor NAD+

enzyme: xanthine dehydrogenase

substrate: xanthine


enzyme_display_name: formate dehydrogenase (NADP(+))

organism: Neomoorella thermoacetica

substrate_name: formate (at 55 degrees Celsius and pH 7.5, with NADP as electron acceptor)

enzyme: formate dehydrogenase (NADP(+))


substrate_name: NAD+

substrate: NAD+


organism: Rhodobacter capsulatus

substrate_name: Xanthine

substrate: Xanthine


enzyme_display_name: nicotinate dehydrogenase

organism: Bacillus sp. (in: firmicutes)

substrate_name: nicotinate

enzyme: nicotinate dehydrogenase

substrate: nicotinate


organism: Escherichia coli (strain K12)

substrate_name: NADH

substrate: NADH


enzyme_display_name: formate dehydrogenase

organism: Methylorubrum extorquens

substrate_name: formate

assay_conditions_summary: pH 7; 303.15 K; cofactor NAD+

enzyme: formate dehydrogenase

substrate: formate


organism: Bacillus subtilis

substrate_name: dihydrodipicolinate

substrate: dihydrodipicolinate


organism: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

substrate_name: NADH (at 30 degrees Celsius)

substrate: NADH (at 30 degrees Celsius)


organism: Lotus japonicus

assay_conditions_summary: pH 7.5; 303.15 K; buffer 0.1 M potassium phosphate; cofactor NADP+

proof_lines: in 0.1 M potassium phosphate buffer, pH 7.5, at 30°C


organism: Thiobacillus sp.

assay_conditions_summary: pH 7; 298.15 K; cofactor NAD+

proof_lines:
at pH 7.0 and 25°C
pH 7.0, 30°C


organism: Thermochaetoides thermophila


organism: Moraxella sp.

assay_conditions_summary: cofactor NAD+


organism: Ogataea angusta

sequence_source: UniProtKB:P33677 direct accession lookup

assay_conditions_summary: pH 6.5; 303.15 K; cofactor NAD+


enzyme_display_name: formate dehydrogenase (cytochrome c-553)

organism: Nitratidesulfovibrio vulgaris


enzyme_display_name: lupanine 17-hydroxylase (cytochrome c)

organism: Pseudomonas sp.

substrate_name: lupanine

enzyme: lupanine 17-hydroxylase (cytochrome c)

substrate: lupanine


enzyme_display_name: ferredoxin--NADP(+) reductase

organism: Chlorobaculum tepidum

substrate_name: NADPH

assay_conditions_summary: pH 7; 298.15 K; buffer 2 mM ferricyanide in 20 mM HEPES-NaOH; cofactor NADP+

proof_lines: with 2 mM ferricyanide in 20 mM HEPES-NaOH buffer, at pH 7.0 and 25°C

enzyme: ferredoxin--NADP(+) reductase

substrate: NADPH


organism: Hydrogenobacter thermophilus

substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

substrate: NADP+


enzyme_display_name: limonene dehydrogenase

organism: Castellaniella defragrans (strain DSM 12143 / CCUG 39792 / 65Phen)

substrate_name: limonene (at 28 degrees Celsius)

enzyme: limonene dehydrogenase

substrate: limonene (at 28 degrees Celsius)


enzyme_display_name: adrenodoxin-NADP(+) reductase

organism: Arabidopsis thaliana

enzyme: adrenodoxin-NADP(+) reductase


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)


enzyme_display_name: alcohol dehydrogenase

organism: Drosophila simulans

enzyme: alcohol dehydrogenase


organism: Drosophila virilis


organism: Homo sapiens

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

smiles: CCCCCCO


substrate_name: Ethanol

smiles: CCO

substrate: Ethanol


organism: Geobacillus stearothermophilus

sequence_source: UniProt REST direct accession lookup: P42328

smiles: C(CO)CO


substrate_name: Retinol

substrate: Retinol


substrate_name: benzyl alcohol

substrate: benzyl alcohol


smiles: CCCCCCCCO


substrate_name: methanol

smiles: CO

substrate: methanol


smiles: CCCCCO


assay_conditions_summary: pH 9; 298.15 K; cofactor NAD+


substrate_name: 12-Hydroxydodecanoic acid

smiles: O=C(O)CCCCCCCCCCCO

substrate: 12-Hydroxydodecanoic acid


organism: Kluyveromyces lactis

assay_conditions_summary: pH 8; 298.15 K; cofactor NADH


assay_conditions_summary: pH 8; 298.15 K; cofactor NADPH


assay_conditions_summary: pH 8; 298.15 K; cofactor NADP+


assay_conditions_summary: pH 8; 298.15 K; cofactor NAD+


substrate_name: Acetaldehyde

smiles: CC=O

substrate: Acetaldehyde


substrate_name: NAD+

substrate: NAD+


organism: Macaca mulatta

assay_conditions_summary: pH 7.3; 303.15 K; cofactor NAD+


assay_conditions_summary: pH 6; 298.15 K; cofactor NAD+


smiles: CCCCO

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NAD+


smiles: CC(C)O


substrate_name: Acetone

smiles: CC(C)=O

substrate: Acetone


organism: Mus musculus

substrate_name: all-trans-Retinal

substrate: all-trans-Retinal


smiles: CCCO


organism: Escherichia coli

substrate_name: ethyl acetoacetate

smiles: CCOC(=O)CC(=O)C

assay_conditions_summary: cofactor NADPH

substrate: ethyl acetoacetate


organism: Mycobacterium tuberculosis

assay_conditions_summary: pH 7.6; 303.15 K; cofactor NADPH


organism: Persea americana

substrate_name: acetoacetyl-CoA

substrate: acetoacetyl-CoA


organism: Saccharomyces cerevisiae

substrate_name: ethanol

assay_conditions_summary: pH 7

substrate: ethanol


enzyme_display_name: carbonyl reductase (NADPH)

organism: Candida magnoliae

substrate_name: Ethyl 4-chloro-3-oxobutanoate; NADPH; H+

enzyme: carbonyl reductase (NADPH)


substrate_name: S-Nitrosoglutathione; NADPH; H+

substrate: S-Nitrosoglutathione; NADPH; H+


substrate_name: 9,10-Phenanthroquinone; H+; NADPH

substrate: 9,10-Phenanthroquinone; H+; NADPH


substrate_name: H+; Menadione; NADPH

substrate: H+; Menadione; NADPH


substrate_name: Menadione; NADPH; H+

substrate: Menadione; NADPH; H+


organism: Mesocricetus auratus

substrate_name: Acetone; NADH; H+

assay_conditions_summary: cofactor NADH

substrate: Acetone; NADH; H+


enzyme_display_name: alcohol dehydrogenase (NADP+)

organism: Cavia porcellus

enzyme: alcohol dehydrogenase (NADP+)


organism: Gallus gallus

substrate_name: H+; NADPH; 4-Pyridinecarboxaldehyde

substrate: H+; NADPH; 4-Pyridinecarboxaldehyde


substrate_name: NADPH; Acetone

substrate: NADPH; Acetone


organism: Bos taurus

substrate_name: 4-Carboxybenzaldehyde; NADPH

substrate: 4-Carboxybenzaldehyde; NADPH


organism: Rattus norvegicus

substrate_name: NADPH; 4-Nitroacetophenone

substrate: NADPH; 4-Nitroacetophenone


organism: Marinobacter nauticus VT8

smiles: CCCCC=CCCCCCCCCCC=O


substrate_name: NADPH; Benzaldehyde

substrate: NADPH; Benzaldehyde


substrate_name: NADPH; H+; Glyceraldehyde

substrate: NADPH; H+; Glyceraldehyde


organism: Pyrococcus furiosus

substrate_name: NADP+

assay_conditions_summary: pH 10; 343.15 K; cofactor NADP+

substrate: NADP+


organism: Mycolicibacterium smegmatis

substrate_name: octanal

smiles: CCCCCCCC=O

substrate: octanal


organism: Sus scrofa

substrate_name: NADP+; 4-Hydroxybutyrate

substrate: NADP+; 4-Hydroxybutyrate


organism: Thermoanaerobacter ethanolicus

substrate_name: NADP+; 2-Butanol

substrate: NADP+; 2-Butanol


organism: Thermococcus sibiricus

substrate_name: D-xylose

assay_conditions_summary: pH 10.5; 333.15 K; cofactor NADP+

substrate: D-xylose


organism: Thermoanaerobacter sp.

substrate_name: NADPH

substrate: NADPH


enzyme_display_name: aldose reductase

organism: Candida tenuis

substrate_name: D-Xylose

assay_conditions_summary: pH 7; 298.15 K; cofactor NADPH

enzyme: aldose reductase

substrate: D-Xylose


substrate_name: NADH

assay_conditions_summary: pH 7; 298.15 K; cofactor NADH

substrate: NADH


organism: Homo sapiens

substrate_name: Acetoacetyl-CoA; H+; NADPH

substrate: Acetoacetyl-CoA; H+; NADPH


organism: Sinomonas cyclohexanicum

assay_conditions_summary: cofactor NAD+


enzyme_display_name: morphine 6-dehydrogenase

organism: Oryctolagus cuniculus

substrate_name: NAD+

enzyme: morphine 6-dehydrogenase

substrate: NAD+


enzyme_display_name: chlordecone reductase

substrate_name: Chlordecone

sequence_source: unresolved accession-backed assignment

enzyme: chlordecone reductase

substrate: Chlordecone


organism: Plasmodium falciparum

substrate_name: acetoacetyl-CoA

assay_conditions_summary: pH 7.5; cofactor NADPH

substrate: acetoacetyl-CoA


organism: Arabidopsis thaliana

substrate_name: farnesol

smiles: CC(=CCCC(=CCCC(=CCO)C)C)C

substrate: farnesol


enzyme_display_name: methylglyoxal reductase (NADPH)

organism: Saccharomyces cerevisiae

enzyme: methylglyoxal reductase (NADPH)


organism: Kluyveromyces marxianus

substrate_name: methyl glyoxal

sequence_source: UniProtKB A0A0E4AX59 REST lookup on 2026-06-01; accession was source-provided by BRENDA in the selected batch.

smiles: CC(=O)C=O

assay_conditions_summary: pH 6; cofactor NADPH

substrate: methyl glyoxal


organism: Candida boidinii

substrate_name: Reduced glutathione; Formaldehyde; NAD+

substrate: Reduced glutathione; Formaldehyde; NAD+


organism: [Candida] boidinii


organism: Canis lupus familiaris

substrate_name: glutathione

smiles: C(CC(=O)NC(CS)C(=O)NCC(=O)O)C(C(=O)O)N

substrate: glutathione


organism: Drosophila melanogaster

substrate_name: S-hydroxymethyl glutathione

substrate: S-hydroxymethyl glutathione


substrate_name: S-(Hydroxymethyl)glutathione

substrate: S-(Hydroxymethyl)glutathione


organism: Komagataella pastoris

substrate_name: formaldehyde

substrate: formaldehyde


organism: Bos taurus

substrate_name: Formaldehyde; NAD+; Reduced glutathione

sequence_source: UniProt REST direct accession lookup for Q3ZC42 on 2026-06-23

substrate: Formaldehyde; NAD+; Reduced glutathione


organism: Taiwanofungus camphoratus

substrate_name: S-nitrosoglutathione

verification_status: verified

sequence_source: UniProt D2CFJ2 FASTA checked directly on 2026-06-23

substrate: S-nitrosoglutathione

status: verified


organism: Deinococcus radiodurans

assay_conditions_summary: pH 7.8; 303.15 K; cofactor NAD+


enzyme_display_name: D-arabinitol dehydrogenase (NADP(+))

organism: Gluconobacter oxydans

substrate_name: glycerol

smiles: C(C(CO)O)O

enzyme: D-arabinitol dehydrogenase (NADP(+))

substrate: glycerol


organism: Thermococcus kodakarensis

substrate_name: homoisocitrate

source_db: skid

source_license: CC BY-NC-ND 4.0

assay_conditions_summary: pH 8; 333.15 K; cofactor NAD+

substrate: homoisocitrate

source: skid


enzyme_display_name: D-2-hydroxyacid dehydrogenase (NAD(+))

organism: Lactobacillus delbrueckii

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NAD+

enzyme: D-2-hydroxyacid dehydrogenase (NAD(+))


enzyme_display_name: homoserine dehydrogenase

organism: Thermus thermophilus

substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

enzyme: homoserine dehydrogenase

substrate: NADP+


organism: Halobacterium mediterranei

substrate_name: 2-Oxoisoleucine; NADH; H+

substrate: 2-Oxoisoleucine; NADH; H+


organism: Corynebacterium sp.

substrate_name: 2-Dehydro-L-idonate; NADPH; H+

substrate: 2-Dehydro-L-idonate; NADPH; H+


enzyme_display_name: geraniol dehydrogenase (NAD(+))

organism: Carpoglyphus lactis

substrate_name: geraniol

smiles: CC(=CCCC(=CCO)C)C

enzyme: geraniol dehydrogenase (NAD(+))

substrate: geraniol


enzyme_display_name: D-chiro-inositol 1-dehydrogenase

organism: Thermotoga maritima

enzyme: D-chiro-inositol 1-dehydrogenase


enzyme_display_name: (R,R)-butanediol dehydrogenase

organism: Clostridium autoethanogenum

substrate_name: (R)-acetoin

enzyme: (R,R)-butanediol dehydrogenase

substrate: (R)-acetoin


substrate_name: acetone

substrate: acetone


smiles: CC(C(C)O)O


substrate_name: acetoin

smiles: CC(=O)C(C)O

substrate: acetoin


smiles: CCC(C)=O


substrate_name: NADPH

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

substrate: NADPH


organism: Rhodococcus erythropolis

substrate_name: diacetyl

smiles: CC(=O)C(C)=O

substrate: diacetyl


substrate_name: NAD+

assay_conditions_summary: pH 6.5; 328.15 K; cofactor NAD+

substrate: NAD+


substrate_name: NADH

assay_conditions_summary: pH 10; 318.15 K; cofactor NADH

substrate: NADH


organism: Saccharomyces cerevisiae

smiles: CCC(O)CO


substrate_name: glycolaldehyde

smiles: O=CCO

substrate: glycolaldehyde


substrate_name: acetaldehyde

smiles: CC=O

substrate: acetaldehyde


smiles: CC(O)C(C)O


enzyme_display_name: isocitrate dehydrogenase (NADP(+))

organism: Mus musculus

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADP+

proof_lines:
Raw SKiD Env: Temp=25°C, pH=7.4
Raw SKiD Env: Temp=-----, pH=7.5

enzyme: isocitrate dehydrogenase (NADP(+))


organism: Desulfotalea psychrophila

assay_conditions_summary: 278.15 K; cofactor NADP+

proof_lines:
Raw SKiD Env: Temp=5°C, pH=-----
Raw SKiD Env: Temp=25°C, pH=-----


enzyme_display_name: levoglucosan dehydrogenase

organism: Arthrobacter sp. I-552

substrate_name: levoglucosan

assay_conditions_summary: pH 9; 313.15 K; cofactor NAD+

enzyme: levoglucosan dehydrogenase

substrate: levoglucosan


organism: Trypanosoma cruzi


enzyme_display_name: D-xylose 1-dehydrogenase (NADP(+), D-xylono-1,4-lactone-forming)

organism: Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)

substrate_name: D-xylose

assay_conditions_summary: cofactor NADPH

substrate: D-xylose


organism: Sus scrofa

proof_lines:
Raw SKiD Env: Temp=25°C, pH=7.4
Raw SKiD Env: Temp=-----, pH=7.4


enzyme_display_name: D-xylose reductase (NADPH)

organism: Meyerozyma guilliermondii

assay_conditions_summary: pH 7.2; 298.15 K; cofactor NADPH

enzyme: D-xylose reductase (NADPH)


organism: Debaryomyces nepalensis

substrate_name: D-Erythrose

assay_conditions_summary: pH 7; 318.15 K; cofactor NADPH

substrate: D-Erythrose


organism: Comamonas testosteroni


organism: Scheffersomyces shehatae

assay_conditions_summary: pH 6.5; 308.15 K; cofactor NADPH


organism: Thermomyces lanuginosus

substrate_name: D-Xylose

assay_conditions_summary: pH 6.5; 323.15 K; cofactor NADPH

substrate: D-Xylose


substrate_name: NAD+; Androsterone

assay_conditions_summary: pH 5.5; 298.15 K; cofactor NAD+

substrate: NAD+; Androsterone


assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADH


organism: Homo sapiens


substrate_name: Dimethylglyoxal

substrate: Dimethylglyoxal


substrate_name: m-Nitrobenzaldehyde

substrate: m-Nitrobenzaldehyde


substrate_name: Benzene dihydrodiol

substrate: Benzene dihydrodiol


substrate_name: Camphorquinone

substrate: Camphorquinone


substrate_name: chenodeoxycholate

substrate: chenodeoxycholate


sequence_source: uniprot_accession (canonical reference only; not validated as the V54L variant)


enzyme_display_name: 7,8-dihydroneopterin oxygenase

organism: Mycobacterium tuberculosis

proof_lines:
at pH 7.0 and 25°C
Raw SKiD Env: Temp=25°C, pH=7

enzyme: 7,8-dihydroneopterin oxygenase


organism: Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)

enzyme: D-xylose 1-dehydrogenase (NADP(+), D-xylono-1,4-lactone-forming)


substrate_name: NAD+; Estradiol-17beta

assay_conditions_summary: cofactor CoA

substrate: NAD+; Estradiol-17beta


substrate_name: NAD+; 5beta-Dihydrotestosterone

assay_conditions_summary: cofactor NAD+

substrate: NAD+; 5beta-Dihydrotestosterone


organism: Pseudomonas sp.

assay_conditions_summary: cofactor NADH


enzyme_display_name: cellobiose dehydrogenase (acceptor)

organism: Chaetomium sp.

substrate_name: lactose

enzyme: cellobiose dehydrogenase (acceptor)

substrate: lactose


organism: Digitalis lanata

substrate_name: NAD+

substrate: NAD+


organism: Amesia atrobrunnea

proof_lines:
at pH 5.0 and 30°C
at pH 6.0 and 30°C


organism: Termitomyces clypeatus

substrate_name: cellotriose

substrate: cellotriose


organism: Dichomera saubinetii

substrate_name: maltose

substrate: maltose


organism: Irpex lacteus

substrate_name: cellobiose

substrate: cellobiose


organism: Termitomyces sp. OE 147


enzyme_display_name: phosphite dehydrogenase

organism: Nostoc punctiforme

substrate_name: phosphonate

assay_conditions_summary: pH 7.3; 298.15 K; cofactor NAD+

enzyme: phosphite dehydrogenase

substrate: phosphonate


organism: Cupriavidus metallidurans


organism: Thermothielavioides terrestris

assay_conditions_summary: pH 6; 298.15 K; buffer 50 mM MES


organism: Stachybotrys bisbyi

proof_lines:
at pH 5.5 and 30°C
at pH 5.´5 and 30°C


enzyme_display_name: gluconate 2-dehydrogenase (acceptor)

organism: Klebsiella pneumoniae

substrate_name: D-gluconate

assay_conditions_summary: pH 4

enzyme: gluconate 2-dehydrogenase (acceptor)

substrate: D-gluconate


organism: Stutzerimonas stutzeri

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADP+

proof_lines:
at pH 7.5 and 30°C
pH 7.2, 30°C
pH 7.2, 40°C


enzyme_display_name: arsenate reductase (thioredoxin)

organism: Bacillus subtilis

substrate_name: arsenate

enzyme: arsenate reductase (thioredoxin)

substrate: arsenate


organism: Thermus thermophilus

sequence_source: UniProt REST Q72HI5 canonical sequence checked 2026-06-04; preserved selected-batch sequence is 150 residues.


assay_conditions_summary: pH 7; 298.15 K; cofactor NAD+


enzyme_display_name: isopenicillin-N synthase

organism: Aspergillus nidulans

enzyme: isopenicillin-N synthase


enzyme_display_name: hercynylcysteine S-oxide synthase

organism: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

substrate_name: hercynine

enzyme: hercynylcysteine S-oxide synthase

substrate: hercynine


enzyme_display_name: reticuline oxidase

organism: Eschscholzia californica

substrate_name: (S)-reticuline

sequence_source: UniProt P30986 PMID-linked direct lookup

assay_conditions_summary: pH 9; 310.15 K; cofactor FAD

enzyme: reticuline oxidase

substrate: (S)-reticuline


enzyme_display_name: iodotyrosine deiodinase

organism: Thermotoga neapolitana

substrate_name: 3-chloro-L-tyrosine; 3-iodo-L-tyrosine; 2-iodophenol; 3-bromo-L-tyrosine

sequence_source: UniProtKB B9K712 direct lookup on 2026-06-09; canonical sequence presence and 186-residue length reconfirmed by direct accession check on 2026-08-26.

proof_lines:
at pH 7.4 and 60°C
at pH 7.4 and 25°C

enzyme: iodotyrosine deiodinase


enzyme_display_name: aureusidin synthase

organism: Antirrhinum majus

substrate_name: isoliquiritigenin

enzyme: aureusidin synthase

substrate: isoliquiritigenin


enzyme_display_name: cannabidiolic acid synthase

organism: Cannabis sativa

substrate_name: cannabigerolate

assay_conditions_summary: pH 5; 303.15 K; buffer 100 mM sodium citrate

proof_lines: in 100 mM sodium citrate buffer (pH 5.0), at 30°C

enzyme: cannabidiolic acid synthase

substrate: cannabigerolate


substrate_name: cannabigerolic acid

sequence_source: source_record; canonical sequence retained in selected batch and not restated in generic review output

substrate: cannabigerolic acid


organism: Sphingobium sp. UG30

sequence_source: UniProtKB:Q937X0 direct FASTA/JSON lookup; canonical sequence is preserved in the selected batch.


enzyme_display_name: thyroxine 5-deiodinase

organism: Homo sapiens

substrate_name: L-thyroxine

enzyme: thyroxine 5-deiodinase

substrate: L-thyroxine


enzyme_display_name: thyroxine 5'-deiodinase

organism: Xenopus laevis

substrate_name: L-thyroxine (outer-ring deiodination activity)

enzyme: thyroxine 5'-deiodinase


organism: Dehalobacter restrictus

substrate_name: tetrachloroethene

smiles: C(=C(Cl)Cl)(Cl)Cl

substrate: tetrachloroethene


organism: Dehalobacter restrictus (strain DSM 9455 / PER-K23)


enzyme_display_name: (+)-pinoresinol reductase

organism: Arabidopsis thaliana

substrate_name: (+)-pinoresinol

enzyme: (+)-pinoresinol reductase

substrate: (+)-pinoresinol


organism: Forsythia intermedia

assay_conditions_summary: cofactor NADPH


enzyme_display_name: (+)-lariciresinol reductase

enzyme: (+)-lariciresinol reductase


organism: Saccharomyces cerevisiae

substrate_name: NAD+


enzyme_display_name: aminobutyraldehyde dehydrogenase

organism: Pseudomonas fluorescens

enzyme: aminobutyraldehyde dehydrogenase


enzyme_display_name: glycolaldehyde dehydrogenase

organism: Escherichia coli

substrate_name: H2O; Glycolaldehyde; NAD+

sequence_source: UniProtKB:P25553 canonical sequence, direct REST lookup 2026-06-24

assay_conditions_summary: cofactor NADP+

enzyme: glycolaldehyde dehydrogenase

substrate: H2O; Glycolaldehyde; NAD+


organism: Arabidopsis sp.


organism: Ctenocephalides felis

substrate_name: pentanal

smiles: CCCCC=O

substrate: pentanal


organism: Saccharolobus solfataricus

assay_conditions_summary: pH 6.5; 343.15 K; cofactor NAD+

proof_lines:
pH 6.5, 70°C
pH 6.5, 70°C, cofactor NAD+

substrate: NAD+


organism: Thermococcus profundus


enzyme_display_name: enoyl-[acyl-carrier-protein] reductase (NADPH, Si-specific)

organism: Bacillus subtilis

substrate_name: NADPH

sequence_source: UniProtKB REST canonical sequence for P71079, checked 2026-06-19

substrate: NADPH


enzyme_display_name: monoamine oxidase

organism: Aspergillus niger

substrate_name: n-butylamine

smiles: CCCCN

enzyme: monoamine oxidase

substrate: n-butylamine


enzyme_display_name: benzoyl-CoA reductase

organism: Thauera aromatica

substrate_name: benzoyl-CoA

assay_conditions_summary: cofactor ATP

enzyme: benzoyl-CoA reductase

substrate: benzoyl-CoA


enzyme_display_name: dihydrofolate reductase

organism: Gallus gallus

assay_conditions_summary: pH 7.4; cofactor NADPH

proof_lines:
pH 7.4
with NADPH
pH 5.0

enzyme: dihydrofolate reductase


organism: Bos taurus

assay_conditions_summary: pH 7.6; 298.15 K; cofactor NADPH


organism: Glycine max


organism: Lactobacillus leichmannii


organism: Geobacillus stearothermophilus

assay_conditions_summary: pH 7; 333.15 K; cofactor NADPH


organism: Moritella profunda

assay_conditions_summary: pH 7; 303.15 K; cofactor NADPH


proof_lines:
at pH 7.0 and 25°C
at pH 7.0 and 15°C
at pH 7.0 and 20°C


organism: Mycolicibacterium phlei


organism: Wuchereria bancrofti


organism: Neisseria gonorrhoeae


organism: Rattus norvegicus


organism: Methylorubrum extorquens


enzyme_display_name: cytokinin dehydrogenase

organism: Oryza sativa subsp. japonica

substrate_name: trans-zeatin

enzyme: cytokinin dehydrogenase

substrate: trans-zeatin


assay_conditions_summary: cofactor FAD


enzyme_display_name: D-proline dehydrogenase

organism: Pyrobaculum islandicum

enzyme: D-proline dehydrogenase


organism: Pyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3)

substrate_name: D-proline

substrate: D-proline


organism: Paenarthrobacter nicotinovorans


organism: Azospirillum sp. B510


enzyme_display_name: leghemoglobin reductase

organism: Phaseolus vulgaris

substrate_name: ferrileghemoglobin

enzyme: leghemoglobin reductase

substrate: ferrileghemoglobin


organism: Vigna unguiculata

substrate_name: H+; NADH; Ferrileghemoglobin

sequence_source: UniProtKB Q9SPB1 direct REST/FASTA accession check on 2026-06-24

assay_conditions_summary: cofactor NADH

substrate: H+; NADH; Ferrileghemoglobin


enzyme_display_name: NAD(P)H oxidase (H2O-forming)

organism: Giardia duodenalis

substrate_name: NADH

assay_conditions_summary: pH 7.8; 295.15 K; cofactor NADPH

enzyme: NAD(P)H oxidase (H2O-forming)

substrate: NADH


organism: Escherichia coli

substrate_name: thio-NAD+

assay_conditions_summary: pH 7.5; 308.15 K; cofactor NADPH

substrate: thio-NAD+


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)


enzyme_display_name: NADH oxidase (H2O2-forming)

organism: Methanocaldococcus jannaschii

assay_conditions_summary: pH 7; 356.15 K; cofactor NADH

enzyme: NADH oxidase (H2O2-forming)


enzyme_display_name: NADH oxidase (H2O-forming)

substrate_name: NADH; O2; H+

sequence_source: UniProtKB P24232 canonical sequence direct lookup

enzyme: NADH oxidase (H2O-forming)

substrate: NADH; O2; H+


organism: Clostridium acetobutylicum

substrate_name: O2; H+; NADH

sequence_source: UniProtKB Q97GC0 canonical sequence direct lookup

substrate: O2; H+; NADH


enzyme_display_name: peroxidase

organism: Burkholderia pseudomallei

enzyme: peroxidase


organism: Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / IAM 19013 / LMG 5710 / NBRC 13948 / NRRL B-527 / VKM B-1787 / 2291 / W)

smiles: O=O


organism: Saccharomyces cerevisiae

substrate_name: NADH; H+; O2

substrate: NADH; H+; O2


organism: Streptococcus mutans

assay_conditions_summary: pH 7; 298.15 K; cofactor NADH


enzyme_display_name: Deleted entry

organism: Enterococcus faecalis

substrate_name: H+; NADH; O2

ki_unit: mM

enzyme: Deleted entry

substrate: H+; NADH; O2


organism: Lactococcus lactis subsp. cremoris


assay_conditions_summary: cofactor NAD+


organism: Thermus thermophilus

substrate_name: FAD; NADH

substrate: FAD; NADH


enzyme_display_name: nitroalkane oxidase

organism: Podospora anserina

enzyme: nitroalkane oxidase


organism: Sus scrofa domestica

substrate_name: 2-Nitropropane; H2O; O2

substrate: 2-Nitropropane; H2O; O2


enzyme_display_name: factor independent urate hydroxylase

organism: Canis lupus familiaris

substrate_name: Urate

enzyme: factor independent urate hydroxylase

substrate: Urate


organism: Arthrobacter globiformis

substrate_name: O2; H2O; Urate

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

sequence_source: UniProtKB:D0VWQ1 direct FASTA/JSON lookup.

substrate: O2; H2O; Urate


organism: Kluyveromyces marxianus


organism: Cyberlindnera jadinii

substrate_name: uric acid

substrate: uric acid


organism: Lysobacter sp. T-15

substrate_name: Urate; O2; H2O

substrate: Urate; O2; H2O


organism: Phaseolus vulgaris


organism: Neurospora crassa


organism: Vicia faba


organism: Puccinia recondita


organism: Streptomyces cyanogenus


enzyme_display_name: ferredoxin--nitrite reductase

organism: Cucurbita pepo

substrate_name: nitrite

enzyme: ferredoxin--nitrite reductase

substrate: nitrite


organism: Vigna unguiculata


organism: Papio sp.


organism: Haloferax mediterranei

substrate_name: reduced methyl viologen

substrate: reduced methyl viologen


organism: Nicotiana tabacum


organism: Leptolyngbya laminosa


organism: Rattus norvegicus

substrate_name: trans-Benzene dihydrodiol

source_license: as published (see paper)

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NAD+

column_label: Table 1, Oxidation section, substrate row 'trans-Benzene dihydrodiol'; TBER1 column 'Km (uM)' = 215; TBER1 column 'kcat (min^-1)' = 38; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.18.

normalized_value_unit_evidence: Km: printed cell 215 uM; 215 / 1000 = 0.215 mM; the exported value is 0.215 mM. kcat: printed cell 38 min^-1; the printed turnover column is per MINUTE, so 38 / 60 = 0.6333333333333333 s^(-1); the exported value is 0.6333333333 s^(-1). kcat/Km: printed cell 0.18 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.18 x 1000 / 60 = 3.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Oxidation row 'trans-Benzene dihydrodiol', TBER1 column group: Km = 215 uM; kcat = 38 min^-1; printed kcat/Km = 0.18 min^-1 uM^-1.

row_label: Table 1, Oxidation section, substrate row 'trans-Benzene dihydrodiol', TBER1 column group

table_label: Table 1: Substrate specificity for non-steroidal substrates in the oxidation and reduction reactions catalyzed by TBER1 and rat 17HSD5 (J. Biochem. 2006, 139(6):1053-1063, journal page 1058)

substrate: trans-Benzene dihydrodiol


column_label: Table 1, Oxidation section, substrate row '2-Cyclohexen-1-ol'; TBER1 column 'Km (uM)' = 1500; TBER1 column 'kcat (min^-1)' = 26; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.02.

normalized_value_unit_evidence: Km: printed cell 1500 uM; 1500 / 1000 = 1.5 mM; the exported value is 1.5 mM. kcat: printed cell 26 min^-1; the printed turnover column is per MINUTE, so 26 / 60 = 0.43333333333333335 s^(-1); the exported value is 0.4333333333 s^(-1). kcat/Km: printed cell 0.02 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.02 x 1000 / 60 = 0.3333333333333333 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Oxidation row '2-Cyclohexen-1-ol', TBER1 column group: Km = 1500 uM; kcat = 26 min^-1; printed kcat/Km = 0.02 min^-1 uM^-1.

row_label: Table 1, Oxidation section, substrate row '2-Cyclohexen-1-ol', TBER1 column group


enzyme_display_name: 17beta-estradiol 17-dehydrogenase

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADP+

column_label: Table 1, Oxidation section, substrate row '2-Cyclohexen-1-ol'; Rat 17HSD5 column 'Km (uM)' = 7000; Rat 17HSD5 column 'kcat (min^-1)' = 3.5; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.0005.

normalized_value_unit_evidence: Km: printed cell 7000 uM; 7000 / 1000 = 7.0 mM; the exported value is 7.0 mM. kcat: printed cell 3.5 min^-1; the printed turnover column is per MINUTE, so 3.5 / 60 = 0.058333333333333334 s^(-1); the exported value is 0.0583333333 s^(-1). kcat/Km: printed cell 0.0005 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.0005 x 1000 / 60 = 0.008333333333333333 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Oxidation row '2-Cyclohexen-1-ol', Rat 17HSD5 column group: Km = 7000 uM; kcat = 3.5 min^-1; printed kcat/Km = 0.0005 min^-1 uM^-1.

row_label: Table 1, Oxidation section, substrate row '2-Cyclohexen-1-ol', Rat 17HSD5 column group

enzyme: 17beta-estradiol 17-dehydrogenase


column_label: Table 1, Oxidation section, substrate row '9a,11a-PGF2'; TBER1 column 'Km (uM)' = 72; TBER1 column 'kcat (min^-1)' = 0.17; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.002.

normalized_value_unit_evidence: Km: printed cell 72 uM; 72 / 1000 = 0.072 mM; the exported value is 0.072 mM. kcat: printed cell 0.17 min^-1; the printed turnover column is per MINUTE, so 0.17 / 60 = 0.0028333333333333335 s^(-1); the exported value is 0.0028333333 s^(-1). kcat/Km: printed cell 0.002 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.002 x 1000 / 60 = 0.03333333333333333 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Oxidation row '9a,11a-PGF2', TBER1 column group: Km = 72 uM; kcat = 0.17 min^-1; printed kcat/Km = 0.002 min^-1 uM^-1.

row_label: Table 1, Oxidation section, substrate row '9a,11a-PGF2', TBER1 column group


column_label: Table 1, Oxidation section, substrate row '9a,11a-PGF2'; Rat 17HSD5 column 'Km (uM)' = 54; Rat 17HSD5 column 'kcat (min^-1)' = 0.51; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.01.

normalized_value_unit_evidence: Km: printed cell 54 uM; 54 / 1000 = 0.054 mM; the exported value is 0.054 mM. kcat: printed cell 0.51 min^-1; the printed turnover column is per MINUTE, so 0.51 / 60 = 0.0085 s^(-1); the exported value is 0.0085 s^(-1). kcat/Km: printed cell 0.01 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.01 x 1000 / 60 = 0.16666666666666666 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Oxidation row '9a,11a-PGF2', Rat 17HSD5 column group: Km = 54 uM; kcat = 0.51 min^-1; printed kcat/Km = 0.01 min^-1 uM^-1.

row_label: Table 1, Oxidation section, substrate row '9a,11a-PGF2', Rat 17HSD5 column group


column_label: Table 1, Reduction section, substrate row '9,10-Phenanthrenequinone'; TBER1 column 'Km (uM)' = 0.4; TBER1 column 'kcat (min^-1)' = 157; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 393.

normalized_value_unit_evidence: Km: printed cell 0.4 uM; 0.4 / 1000 = 0.0004 mM; the exported value is 0.0004 mM. kcat: printed cell 157 min^-1; the printed turnover column is per MINUTE, so 157 / 60 = 2.6166666666666667 s^(-1); the exported value is 2.6166666667 s^(-1). kcat/Km: printed cell 393 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 393 x 1000 / 60 = 6550.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row '9,10-Phenanthrenequinone', TBER1 column group: Km = 0.4 uM; kcat = 157 min^-1; printed kcat/Km = 393 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row '9,10-Phenanthrenequinone', TBER1 column group


substrate_name: TBE

smiles: C(C(Br)Br)(Br)Br

column_label: Table 1, Reduction section, substrate row 'TBE'; TBER1 column 'Km (uM)' = 180; TBER1 column 'kcat (min^-1)' = 75; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.42.

normalized_value_unit_evidence: Km: printed cell 180 uM; 180 / 1000 = 0.18 mM; the exported value is 0.18 mM. kcat: printed cell 75 min^-1; the printed turnover column is per MINUTE, so 75 / 60 = 1.25 s^(-1); the exported value is 1.25 s^(-1). kcat/Km: printed cell 0.42 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.42 x 1000 / 60 = 7.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row 'TBE', TBER1 column group: Km = 180 uM; kcat = 75 min^-1; printed kcat/Km = 0.42 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row 'TBE', TBER1 column group

substrate: TBE


column_label: Table 1, Reduction section, substrate row 'TBE'; Rat 17HSD5 column 'Km (uM)' = 16; Rat 17HSD5 column 'kcat (min^-1)' = 9.2; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.58.

normalized_value_unit_evidence: Km: printed cell 16 uM; 16 / 1000 = 0.016 mM; the exported value is 0.016 mM. kcat: printed cell 9.2 min^-1; the printed turnover column is per MINUTE, so 9.2 / 60 = 0.15333333333333332 s^(-1); the exported value is 0.1533333333 s^(-1). kcat/Km: printed cell 0.58 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.58 x 1000 / 60 = 9.666666666666666 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row 'TBE', Rat 17HSD5 column group: Km = 16 uM; kcat = 9.2 min^-1; printed kcat/Km = 0.58 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row 'TBE', Rat 17HSD5 column group


smiles: CCC(=O)C(=O)C

column_label: Table 1, Reduction section, substrate row '2,3-Pentanedione'; TBER1 column 'Km (uM)' = 385; TBER1 column 'kcat (min^-1)' = 29; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.08.

normalized_value_unit_evidence: Km: printed cell 385 uM; 385 / 1000 = 0.385 mM; the exported value is 0.385 mM. kcat: printed cell 29 min^-1; the printed turnover column is per MINUTE, so 29 / 60 = 0.48333333333333334 s^(-1); the exported value is 0.4833333333 s^(-1). kcat/Km: printed cell 0.08 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.08 x 1000 / 60 = 1.3333333333333333 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row '2,3-Pentanedione', TBER1 column group: Km = 385 uM; kcat = 29 min^-1; printed kcat/Km = 0.08 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row '2,3-Pentanedione', TBER1 column group


column_label: Table 1, Reduction section, substrate row '2,3-Pentanedione'; Rat 17HSD5 column 'Km (uM)' = 57; Rat 17HSD5 column 'kcat (min^-1)' = 8.8; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.15.

normalized_value_unit_evidence: Km: printed cell 57 uM; 57 / 1000 = 0.057 mM; the exported value is 0.057 mM. kcat: printed cell 8.8 min^-1; the printed turnover column is per MINUTE, so 8.8 / 60 = 0.14666666666666667 s^(-1); the exported value is 0.1466666667 s^(-1). kcat/Km: printed cell 0.15 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.15 x 1000 / 60 = 2.5 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row '2,3-Pentanedione', Rat 17HSD5 column group: Km = 57 uM; kcat = 8.8 min^-1; printed kcat/Km = 0.15 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row '2,3-Pentanedione', Rat 17HSD5 column group


smiles: CC(=O)C(=O)C

column_label: Table 1, Reduction section, substrate row '2,3-Butanedione'; TBER1 column 'Km (uM)' = 4800; TBER1 column 'kcat (min^-1)' = 100; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.02.

normalized_value_unit_evidence: Km: printed cell 4800 uM; 4800 / 1000 = 4.8 mM; the exported value is 4.8 mM. kcat: printed cell 100 min^-1; the printed turnover column is per MINUTE, so 100 / 60 = 1.6666666666666667 s^(-1); the exported value is 1.6666666667 s^(-1). kcat/Km: printed cell 0.02 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.02 x 1000 / 60 = 0.3333333333333333 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row '2,3-Butanedione', TBER1 column group: Km = 4800 uM; kcat = 100 min^-1; printed kcat/Km = 0.02 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row '2,3-Butanedione', TBER1 column group


column_label: Table 1, Reduction section, substrate row '2,3-Butanedione'; Rat 17HSD5 column 'Km (uM)' = 126; Rat 17HSD5 column 'kcat (min^-1)' = 16; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.13.

normalized_value_unit_evidence: Km: printed cell 126 uM; 126 / 1000 = 0.126 mM; the exported value is 0.126 mM. kcat: printed cell 16 min^-1; the printed turnover column is per MINUTE, so 16 / 60 = 0.26666666666666666 s^(-1); the exported value is 0.2666666667 s^(-1). kcat/Km: printed cell 0.13 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.13 x 1000 / 60 = 2.1666666666666665 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row '2,3-Butanedione', Rat 17HSD5 column group: Km = 126 uM; kcat = 16 min^-1; printed kcat/Km = 0.13 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row '2,3-Butanedione', Rat 17HSD5 column group


substrate_name: Acetylpyridine

column_label: Table 1, Reduction section, substrate row 'Acetylpyridine'; Rat 17HSD5 column 'Km (uM)' = 266; Rat 17HSD5 column 'kcat (min^-1)' = 4.5; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.02.

normalized_value_unit_evidence: Km: printed cell 266 uM; 266 / 1000 = 0.266 mM; the exported value is 0.266 mM. kcat: printed cell 4.5 min^-1; the printed turnover column is per MINUTE, so 4.5 / 60 = 0.075 s^(-1); the exported value is 0.075 s^(-1). kcat/Km: printed cell 0.02 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.02 x 1000 / 60 = 0.3333333333333333 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row 'Acetylpyridine', Rat 17HSD5 column group: Km = 266 uM; kcat = 4.5 min^-1; printed kcat/Km = 0.02 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row 'Acetylpyridine', Rat 17HSD5 column group

substrate: Acetylpyridine


column_label: Table 2, (A) section, substrate row '5b-Androstane-3a,17b-diol'; TBER1 column 'Km (uM)' = 3.5; TBER1 column 'kcat (min^-1)' = 28; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 8.1.

normalized_value_unit_evidence: Km: printed cell 3.5 uM; 3.5 / 1000 = 0.0035 mM; the exported value is 0.0035 mM. kcat: printed cell 28 min^-1; the printed turnover column is per MINUTE, so 28 / 60 = 0.4666666666666667 s^(-1); the exported value is 0.4666666667 s^(-1). kcat/Km: printed cell 8.1 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 8.1 x 1000 / 60 = 135.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row '5b-Androstane-3a,17b-diol', TBER1 column group: Km = 3.5 uM; kcat = 28 min^-1; printed kcat/Km = 8.1 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row '5b-Androstane-3a,17b-diol', TBER1 column group

table_label: Table 2: Substrate specificity for steroids in the oxidation and reduction reactions catalyzed by TBER1 and rat 17HSD5 (J. Biochem. 2006, 139(6):1053-1063, journal page 1058)


column_label: Table 2, (A) section, substrate row '5b-Androstane-3a,17b-diol'; Rat 17HSD5 column 'Km (uM)' = 10; Rat 17HSD5 column 'kcat (min^-1)' = 7.8; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.78.

normalized_value_unit_evidence: Km: printed cell 10 uM; 10 / 1000 = 0.01 mM; the exported value is 0.01 mM. kcat: printed cell 7.8 min^-1; the printed turnover column is per MINUTE, so 7.8 / 60 = 0.13 s^(-1); the exported value is 0.13 s^(-1). kcat/Km: printed cell 0.78 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.78 x 1000 / 60 = 13.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row '5b-Androstane-3a,17b-diol', Rat 17HSD5 column group: Km = 10 uM; kcat = 7.8 min^-1; printed kcat/Km = 0.78 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row '5b-Androstane-3a,17b-diol', Rat 17HSD5 column group


column_label: Table 2, (A) section, substrate row '5a-Dihydrotestosterone'; TBER1 column 'Km (uM)' = 9.1; TBER1 column 'kcat (min^-1)' = 12; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 1.30.

normalized_value_unit_evidence: Km: printed cell 9.1 uM; 9.1 / 1000 = 0.0091 mM; the exported value is 0.0091 mM. kcat: printed cell 12 min^-1; the printed turnover column is per MINUTE, so 12 / 60 = 0.2 s^(-1); the exported value is 0.2 s^(-1). kcat/Km: printed cell 1.30 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 1.30 x 1000 / 60 = 21.666666666666668 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row '5a-Dihydrotestosterone', TBER1 column group: Km = 9.1 uM; kcat = 12 min^-1; printed kcat/Km = 1.30 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row '5a-Dihydrotestosterone', TBER1 column group


substrate_name: Testosterone

column_label: Table 2, (A) section, substrate row 'Testosterone'; TBER1 column 'Km (uM)' = 25; TBER1 column 'kcat (min^-1)' = 16; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.62.

normalized_value_unit_evidence: Km: printed cell 25 uM; 25 / 1000 = 0.025 mM; the exported value is 0.025 mM. kcat: printed cell 16 min^-1; the printed turnover column is per MINUTE, so 16 / 60 = 0.26666666666666666 s^(-1); the exported value is 0.2666666667 s^(-1). kcat/Km: printed cell 0.62 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.62 x 1000 / 60 = 10.333333333333334 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row 'Testosterone', TBER1 column group: Km = 25 uM; kcat = 16 min^-1; printed kcat/Km = 0.62 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row 'Testosterone', TBER1 column group

substrate: Testosterone


column_label: Table 2, (A) section, substrate row 'Testosterone'; Rat 17HSD5 column 'Km (uM)' = 21; Rat 17HSD5 column 'kcat (min^-1)' = 21; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 1.0.

normalized_value_unit_evidence: Km: printed cell 21 uM; 21 / 1000 = 0.021 mM; the exported value is 0.021 mM. kcat: printed cell 21 min^-1; the printed turnover column is per MINUTE, so 21 / 60 = 0.35 s^(-1); the exported value is 0.35 s^(-1). kcat/Km: printed cell 1.0 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 1.0 x 1000 / 60 = 16.666666666666668 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row 'Testosterone', Rat 17HSD5 column group: Km = 21 uM; kcat = 21 min^-1; printed kcat/Km = 1.0 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row 'Testosterone', Rat 17HSD5 column group


column_label: Table 2, (A) section, substrate row '17b-Estradiol'; TBER1 column 'Km (uM)' = 33; TBER1 column 'kcat (min^-1)' = 9.0; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.27.

normalized_value_unit_evidence: Km: printed cell 33 uM; 33 / 1000 = 0.033 mM; the exported value is 0.033 mM. kcat: printed cell 9.0 min^-1; the printed turnover column is per MINUTE, so 9.0 / 60 = 0.15 s^(-1); the exported value is 0.15 s^(-1). kcat/Km: printed cell 0.27 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.27 x 1000 / 60 = 4.5 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row '17b-Estradiol', TBER1 column group: Km = 33 uM; kcat = 9.0 min^-1; printed kcat/Km = 0.27 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row '17b-Estradiol', TBER1 column group


column_label: Table 2, (A) section, substrate row '17b-Estradiol'; Rat 17HSD5 column 'Km (uM)' = 14; Rat 17HSD5 column 'kcat (min^-1)' = 25; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 1.8.

normalized_value_unit_evidence: Km: printed cell 14 uM; 14 / 1000 = 0.014 mM; the exported value is 0.014 mM. kcat: printed cell 25 min^-1; the printed turnover column is per MINUTE, so 25 / 60 = 0.4166666666666667 s^(-1); the exported value is 0.4166666667 s^(-1). kcat/Km: printed cell 1.8 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 1.8 x 1000 / 60 = 30.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row '17b-Estradiol', Rat 17HSD5 column group: Km = 14 uM; kcat = 25 min^-1; printed kcat/Km = 1.8 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row '17b-Estradiol', Rat 17HSD5 column group


column_label: Table 2, (A) section, substrate row '5a-Androstane-3a,17b-diol'; TBER1 column 'Km (uM)' = 17; TBER1 column 'kcat (min^-1)' = 4.2; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.25.

normalized_value_unit_evidence: Km: printed cell 17 uM; 17 / 1000 = 0.017 mM; the exported value is 0.017 mM. kcat: printed cell 4.2 min^-1; the printed turnover column is per MINUTE, so 4.2 / 60 = 0.07 s^(-1); the exported value is 0.07 s^(-1). kcat/Km: printed cell 0.25 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.25 x 1000 / 60 = 4.166666666666667 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row '5a-Androstane-3a,17b-diol', TBER1 column group: Km = 17 uM; kcat = 4.2 min^-1; printed kcat/Km = 0.25 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row '5a-Androstane-3a,17b-diol', TBER1 column group


column_label: Table 2, (B) section, substrate row '5a-Pregnan-20a-ol-3-one'; TBER1 column 'Km (uM)' = 5.1; TBER1 column 'kcat (min^-1)' = 2.4; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.47.

normalized_value_unit_evidence: Km: printed cell 5.1 uM; 5.1 / 1000 = 0.0050999999999999995 mM; the exported value is 0.0050999999999999995 mM. kcat: printed cell 2.4 min^-1; the printed turnover column is per MINUTE, so 2.4 / 60 = 0.04 s^(-1); the exported value is 0.04 s^(-1). kcat/Km: printed cell 0.47 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.47 x 1000 / 60 = 7.833333333333333 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (B) row '5a-Pregnan-20a-ol-3-one', TBER1 column group: Km = 5.1 uM; kcat = 2.4 min^-1; printed kcat/Km = 0.47 min^-1 uM^-1.

row_label: Table 2, (B) section, substrate row '5a-Pregnan-20a-ol-3-one', TBER1 column group


column_label: Table 2, (B) section, substrate row '5b-Pregnan-3a-ol-20-one'; TBER1 column 'Km (uM)' = 8.4; TBER1 column 'kcat (min^-1)' = 0.83; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.10.

normalized_value_unit_evidence: Km: printed cell 8.4 uM; 8.4 / 1000 = 0.008400000000000001 mM; the exported value is 0.008400000000000001 mM. kcat: printed cell 0.83 min^-1; the printed turnover column is per MINUTE, so 0.83 / 60 = 0.013833333333333333 s^(-1); the exported value is 0.0138333333 s^(-1). kcat/Km: printed cell 0.10 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.10 x 1000 / 60 = 1.6666666666666667 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (B) row '5b-Pregnan-3a-ol-20-one', TBER1 column group: Km = 8.4 uM; kcat = 0.83 min^-1; printed kcat/Km = 0.10 min^-1 uM^-1.

row_label: Table 2, (B) section, substrate row '5b-Pregnan-3a-ol-20-one', TBER1 column group


column_label: Table 2, (C) section, substrate row '5b-Androstan-3a-ol-17-one'; Rat 17HSD5 column 'Km (uM)' = 6.7; Rat 17HSD5 column 'kcat (min^-1)' = 1.3; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.20.

normalized_value_unit_evidence: Km: printed cell 6.7 uM; 6.7 / 1000 = 0.0067 mM; the exported value is 0.0067 mM. kcat: printed cell 1.3 min^-1; the printed turnover column is per MINUTE, so 1.3 / 60 = 0.021666666666666667 s^(-1); the exported value is 0.0216666667 s^(-1). kcat/Km: printed cell 0.20 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.20 x 1000 / 60 = 3.3333333333333335 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (C) row '5b-Androstan-3a-ol-17-one', Rat 17HSD5 column group (the second of the two section (C) rows carrying the substrate label '5b-Androstan-3a-ol-17-one'; it is the row that prints Rat 17HSD5 cells, the first printing NS in the Rat 17HSD5 group): Km = 6.7 uM; kcat = 1.3 min^-1; printed kcat/Km = 0.20 min^-1 uM^-1.

row_label: Table 2, (C) section, substrate row '5b-Androstan-3a-ol-17-one', Rat 17HSD5 column group


column_label: Table 2, (C) section, substrate row '5b-Androstane-3,17-dione'; TBER1 column 'Km (uM)' = 19; TBER1 column 'kcat (min^-1)' = 3.2; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.17.

normalized_value_unit_evidence: Km: printed cell 19 uM; 19 / 1000 = 0.019 mM; the exported value is 0.019 mM. kcat: printed cell 3.2 min^-1; the printed turnover column is per MINUTE, so 3.2 / 60 = 0.05333333333333334 s^(-1); the exported value is 0.0533333333 s^(-1). kcat/Km: printed cell 0.17 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.17 x 1000 / 60 = 2.8333333333333335 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (C) row '5b-Androstane-3,17-dione', TBER1 column group: Km = 19 uM; kcat = 3.2 min^-1; printed kcat/Km = 0.17 min^-1 uM^-1.

row_label: Table 2, (C) section, substrate row '5b-Androstane-3,17-dione', TBER1 column group


column_label: Table 2, (C) section, substrate row '5a-Androstane-3,17-dione'; TBER1 column 'Km (uM)' = 18; TBER1 column 'kcat (min^-1)' = 1.3; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.07.

normalized_value_unit_evidence: Km: printed cell 18 uM; 18 / 1000 = 0.018 mM; the exported value is 0.018 mM. kcat: printed cell 1.3 min^-1; the printed turnover column is per MINUTE, so 1.3 / 60 = 0.021666666666666667 s^(-1); the exported value is 0.0216666667 s^(-1). kcat/Km: printed cell 0.07 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.07 x 1000 / 60 = 1.1666666666666667 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (C) row '5a-Androstane-3,17-dione', TBER1 column group: Km = 18 uM; kcat = 1.3 min^-1; printed kcat/Km = 0.07 min^-1 uM^-1.

row_label: Table 2, (C) section, substrate row '5a-Androstane-3,17-dione', TBER1 column group


column_label: Table 2, (C) section, substrate row '4-Androstene-3,17-dione'; TBER1 column 'Km (uM)' = 17; TBER1 column 'kcat (min^-1)' = 1.0; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.06.

normalized_value_unit_evidence: Km: printed cell 17 uM; 17 / 1000 = 0.017 mM; the exported value is 0.017 mM. kcat: printed cell 1.0 min^-1; the printed turnover column is per MINUTE, so 1.0 / 60 = 0.016666666666666666 s^(-1); the exported value is 0.0166666667 s^(-1). kcat/Km: printed cell 0.06 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.06 x 1000 / 60 = 1.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (C) row '4-Androstene-3,17-dione', TBER1 column group: Km = 17 uM; kcat = 1.0 min^-1; printed kcat/Km = 0.06 min^-1 uM^-1.

row_label: Table 2, (C) section, substrate row '4-Androstene-3,17-dione', TBER1 column group


substrate_name: Dehydroepiandrosterone

column_label: Table 2, (C) section, substrate row 'Dehydroepiandrosterone'; Rat 17HSD5 column 'Km (uM)' = 11; Rat 17HSD5 column 'kcat (min^-1)' = 5.0; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.45.

normalized_value_unit_evidence: Km: printed cell 11 uM; 11 / 1000 = 0.011 mM; the exported value is 0.011 mM. kcat: printed cell 5.0 min^-1; the printed turnover column is per MINUTE, so 5.0 / 60 = 0.08333333333333333 s^(-1); the exported value is 0.0833333333 s^(-1). kcat/Km: printed cell 0.45 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.45 x 1000 / 60 = 7.5 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (C) row 'Dehydroepiandrosterone', Rat 17HSD5 column group: Km = 11 uM; kcat = 5.0 min^-1; printed kcat/Km = 0.45 min^-1 uM^-1.

row_label: Table 2, (C) section, substrate row 'Dehydroepiandrosterone', Rat 17HSD5 column group

substrate: Dehydroepiandrosterone


column_label: Table 2, (D) section, substrate row '17a-Hydroxyprogesterone'; Rat 17HSD5 column 'Km (uM)' = 50; Rat 17HSD5 column 'kcat (min^-1)' = 6.2; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.12.

normalized_value_unit_evidence: Km: printed cell 50 uM; 50 / 1000 = 0.05 mM; the exported value is 0.05 mM. kcat: printed cell 6.2 min^-1; the printed turnover column is per MINUTE, so 6.2 / 60 = 0.10333333333333333 s^(-1); the exported value is 0.1033333333 s^(-1). kcat/Km: printed cell 0.12 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.12 x 1000 / 60 = 2.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (D) row '17a-Hydroxyprogesterone', Rat 17HSD5 column group: Km = 50 uM; kcat = 6.2 min^-1; printed kcat/Km = 0.12 min^-1 uM^-1.

row_label: Table 2, (D) section, substrate row '17a-Hydroxyprogesterone', Rat 17HSD5 column group


column_label: Table 2, (E) section, substrate row '16-Ketoestrone'; TBER1 column 'Km (uM)' = 0.4; TBER1 column 'kcat (min^-1)' = 113; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 283.

normalized_value_unit_evidence: Km: printed cell 0.4 uM; 0.4 / 1000 = 0.0004 mM; the exported value is 0.0004 mM. kcat: printed cell 113 min^-1; the printed turnover column is per MINUTE, so 113 / 60 = 1.8833333333333333 s^(-1); the exported value is 1.8833333333 s^(-1). kcat/Km: printed cell 283 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 283 x 1000 / 60 = 4716.666666666667 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (E) row '16-Ketoestrone', TBER1 column group: Km = 0.4 uM; kcat = 113 min^-1; printed kcat/Km = 283 min^-1 uM^-1.

row_label: Table 2, (E) section, substrate row '16-Ketoestrone', TBER1 column group


column_label: Table 2, (E) section, substrate row '16-Ketoestrone'; Rat 17HSD5 column 'Km (uM)' = 0.7; Rat 17HSD5 column 'kcat (min^-1)' = 5.2; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 7.5.

normalized_value_unit_evidence: Km: printed cell 0.7 uM; 0.7 / 1000 = 0.0007 mM; the exported value is 0.0007 mM. kcat: printed cell 5.2 min^-1; the printed turnover column is per MINUTE, so 5.2 / 60 = 0.08666666666666667 s^(-1); the exported value is 0.0866666667 s^(-1). kcat/Km: printed cell 7.5 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 7.5 x 1000 / 60 = 125.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (E) row '16-Ketoestrone', Rat 17HSD5 column group: Km = 0.7 uM; kcat = 5.2 min^-1; printed kcat/Km = 7.5 min^-1 uM^-1.

row_label: Table 2, (E) section, substrate row '16-Ketoestrone', Rat 17HSD5 column group


column_label: Table 2, (E) section, substrate row '21-Dehydrocortisol'; Rat 17HSD5 column 'Km (uM)' = 2.8; Rat 17HSD5 column 'kcat (min^-1)' = 7.2; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 2.6.

normalized_value_unit_evidence: Km: printed cell 2.8 uM; 2.8 / 1000 = 0.0028 mM; the exported value is 0.0028 mM. kcat: printed cell 7.2 min^-1; the printed turnover column is per MINUTE, so 7.2 / 60 = 0.12000000000000001 s^(-1); the exported value is 0.12 s^(-1). kcat/Km: printed cell 2.6 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 2.6 x 1000 / 60 = 43.333333333333336 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (E) row '21-Dehydrocortisol', Rat 17HSD5 column group: Km = 2.8 uM; kcat = 7.2 min^-1; printed kcat/Km = 2.6 min^-1 uM^-1.

row_label: Table 2, (E) section, substrate row '21-Dehydrocortisol', Rat 17HSD5 column group


assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADPH

column_label: Journal page 1059, the printed Km and kcat for the NADPH-linked reduction of TBE by the recombinant rat 3a-HSD: Km = 23 uM; kcat = 24 min^-1. The paper prints no kcat/Km value for this measurement.

normalized_value_unit_evidence: Km: printed cell 23 uM; 23 / 1000 = 0.023 mM; the exported value is 0.023 mM. kcat: printed cell 24 min^-1; the printed turnover column is per MINUTE, so 24 / 60 = 0.4 s^(-1); the exported value is 0.4 s^(-1). kcat/Km: the paper prints no catalytic-efficiency value for this measurement and this catalog row carries none either, so the ratio is typed as the same-measurement quotient of the two printed cells, 0.4 / 0.023 = 17.39130434782609 s^(-1)*mM^(-1), and no ratio is shipped.

raw_value_unit_evidence: journal page 1059, the printed Km and kcat for the NADPH-linked reduction of TBE by the recombinant rat 3a-HSD: Km = 23 uM; kcat = 24 min^-1; the paper prints no kcat/Km value for this measurement.

table_label: Identity of TBER2 with 3a-HSD: the printed kinetic constants of the recombinant rat 3a-HSD (J. Biochem. 2006, 139(6):1053-1063, journal page 1059)


substrate_name: trans-benzene dihydrodiol

column_label: Journal page 1059, the printed Km and kcat for the oxidation of trans-benzene dihydrodiol by the recombinant rat 3a-HSD: Km = 0.37 mM; kcat = 3.6 min^-1. The paper prints no kcat/Km value for this measurement.

normalized_value_unit_evidence: Km: printed cell 0.37 mM; 0.37 mM needs no conversion; the exported value is 0.37 mM. kcat: printed cell 3.6 min^-1; the printed turnover column is per MINUTE, so 3.6 / 60 = 0.060000000000000005 s^(-1); the exported value is 0.06 s^(-1). kcat/Km: the paper prints no catalytic-efficiency value for this measurement and this catalog row carries none either, so the ratio is typed as the same-measurement quotient of the two printed cells, 0.060000000000000005 / 0.37 = 0.16216216216216217 s^(-1)*mM^(-1), and no ratio is shipped.

raw_value_unit_evidence: journal page 1059, the printed Km and kcat for the oxidation of trans-benzene dihydrodiol by the recombinant rat 3a-HSD: Km = 0.37 mM; kcat = 3.6 min^-1; the paper prints no kcat/Km value for this measurement.

substrate: trans-benzene dihydrodiol


enzyme_display_name: 4-hydroxyproline epimerase

organism: Paracoccus denitrificans

enzyme: 4-hydroxyproline epimerase


organism: Shewanella loihica


organism: Xanthomonas campestris


organism: Ochrobactrum anthropi


organism: Agrobacterium tumefaciens


organism: Burkholderia ambifaria


organism: Burkholderia multivorans


organism: Bacillus cereus


organism: Burkholderia thailandensis


enzyme_display_name: alcohol dehydrogenase (NADP+)

organism: Homo sapiens

enzyme: alcohol dehydrogenase (NADP+)


enzyme_display_name: beta-lactamase

organism: Stenotrophomonas maltophilia

substrate_name: Carbenicillin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Carbenicillin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 194 +/- 15; column 'kcat (s-1)' = 277; column 'kcat/Km (M-1 s-1)' = 1.40 x 10^6.

normalized_value_unit_evidence: Km: printed Table 3 cell 194 uM; 194 / 1000 = 0.194 mM; the exported value is 0.194 mM. kcat: printed Table 3 cell 277 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 277 s^(-1). kcat/Km: printed Table 3 cell 1.40 x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1400000 / 1000 = 1400 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Carbenicillin': Km = 194 +/- 15 uM; kcat = 277 s^-1; printed kcat/Km = 1.40 x 10^6 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Carbenicillin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

table_label: Table 3: Kinetic parameters determined for class B beta-lactamases (Biochemical Journal 1993, 291:151-155)

enzyme: beta-lactamase

substrate: Carbenicillin


substrate_name: Penicillin G

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Penicillin G' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 50 +/- 6; column 'kcat (s-1)' = 1100; column 'kcat/Km (M-1 s-1)' = 2.20 x 10^7.

normalized_value_unit_evidence: Km: printed Table 3 cell 50 uM; 50 / 1000 = 0.05 mM; the exported value is 0.05 mM. kcat: printed Table 3 cell 1100 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 1100 s^(-1). kcat/Km: printed Table 3 cell 2.20 x 10^7 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 22000000 / 1000 = 22000 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Penicillin G': Km = 50 +/- 6 uM; kcat = 1100 s^-1; printed kcat/Km = 2.20 x 10^7 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Penicillin G' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Penicillin G


substrate_name: Oxacillin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Oxacillin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 27 +/- 3; column 'kcat (s-1)' = 285; column 'kcat/Km (M-1 s-1)' = 1.10 x 10^7.

normalized_value_unit_evidence: Km: printed Table 3 cell 27 uM; 27 / 1000 = 0.027 mM; the exported value is 0.027 mM. kcat: printed Table 3 cell 285 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 285 s^(-1). kcat/Km: printed Table 3 cell 1.10 x 10^7 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 11000000 / 1000 = 11000 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Oxacillin': Km = 27 +/- 3 uM; kcat = 285 s^-1; printed kcat/Km = 1.10 x 10^7 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Oxacillin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Oxacillin


substrate_name: Cephaloridin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cephaloridin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 300 +/- 50; column 'kcat (s-1)' = 28; column 'kcat/Km (M-1 s-1)' = 9.30 x 10^4.

normalized_value_unit_evidence: Km: printed Table 3 cell 300 uM; 300 / 1000 = 0.3 mM; the exported value is 0.3 mM. kcat: printed Table 3 cell 28 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 28 s^(-1). kcat/Km: printed Table 3 cell 9.30 x 10^4 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 93000 / 1000 = 93 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Cephaloridin': Km = 300 +/- 50 uM; kcat = 28 s^-1; printed kcat/Km = 9.30 x 10^4 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cephaloridin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Cephaloridin


substrate_name: Cephalexin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cephalexin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 100 +/- 8; column 'kcat (s-1)' = 37; column 'kcat/Km (M-1 s-1)' = 3.70 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 100 uM; 100 / 1000 = 0.1 mM; the exported value is 0.1 mM. kcat: printed Table 3 cell 37 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 37 s^(-1). kcat/Km: printed Table 3 cell 3.70 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 370000 / 1000 = 370 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Cephalexin': Km = 100 +/- 8 uM; kcat = 37 s^-1; printed kcat/Km = 3.70 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cephalexin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Cephalexin


substrate_name: Cephaloglycin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cephaloglycin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 36 +/- 2; column 'kcat (s-1)' = 19; column 'kcat/Km (M-1 s-1)' = 5.30 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 36 uM; 36 / 1000 = 0.036 mM; the exported value is 0.036 mM. kcat: printed Table 3 cell 19 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 19 s^(-1). kcat/Km: printed Table 3 cell 5.30 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 530000 / 1000 = 530 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Cephaloglycin': Km = 36 +/- 2 uM; kcat = 19 s^-1; printed kcat/Km = 5.30 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cephaloglycin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Cephaloglycin


substrate_name: Nitrocefin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Nitrocefin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 7 +/- 1; column 'kcat (s-1)' = 20; column 'kcat/Km (M-1 s-1)' = 2.90 x 10^6.

normalized_value_unit_evidence: Km: printed Table 3 cell 7 uM; 7 / 1000 = 0.007 mM; the exported value is 0.007 mM. kcat: printed Table 3 cell 20 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 20 s^(-1). kcat/Km: printed Table 3 cell 2.90 x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 2900000 / 1000 = 2900 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Nitrocefin': Km = 7 +/- 1 uM; kcat = 20 s^-1; printed kcat/Km = 2.90 x 10^6 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Nitrocefin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Nitrocefin


substrate_name: Cefuroxime

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cefuroxime' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 30 +/- 3; column 'kcat (s-1)' = 80; column 'kcat/Km (M-1 s-1)' = 2.70 x 10^6.

normalized_value_unit_evidence: Km: printed Table 3 cell 30 uM; 30 / 1000 = 0.03 mM; the exported value is 0.03 mM. kcat: printed Table 3 cell 80 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 80 s^(-1). kcat/Km: printed Table 3 cell 2.70 x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 2700000 / 1000 = 2700 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Cefuroxime': Km = 30 +/- 3 uM; kcat = 80 s^-1; printed kcat/Km = 2.70 x 10^6 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cefuroxime' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Cefuroxime


substrate_name: Cefotaxime

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cefotaxime' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 26 +/- 3; column 'kcat (s-1)' = 66; column 'kcat/Km (M-1 s-1)' = 2.60 x 10^6.

normalized_value_unit_evidence: Km: printed Table 3 cell 26 uM; 26 / 1000 = 0.026 mM; the exported value is 0.026 mM. kcat: printed Table 3 cell 66 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 66 s^(-1). kcat/Km: printed Table 3 cell 2.60 x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 2600000 / 1000 = 2600 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Cefotaxime': Km = 26 +/- 3 uM; kcat = 66 s^-1; printed kcat/Km = 2.60 x 10^6 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cefotaxime' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Cefotaxime


substrate_name: Imipenem

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Imipenem' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 90 +/- 8; column 'kcat (s-1)' = 65; column 'kcat/Km (M-1 s-1)' = 7.30 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 90 uM; 90 / 1000 = 0.09 mM; the exported value is 0.09 mM. kcat: printed Table 3 cell 65 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 65 s^(-1). kcat/Km: printed Table 3 cell 7.30 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 730000 / 1000 = 730 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Imipenem': Km = 90 +/- 8 uM; kcat = 65 s^-1; printed kcat/Km = 7.30 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Imipenem' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Imipenem


substrate_name: Sulbactam

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Sulbactam' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 76 +/- 4; column 'kcat (s-1)' = 210; column 'kcat/Km (M-1 s-1)' = 2.80 x 10^6.

normalized_value_unit_evidence: Km: printed Table 3 cell 76 uM; 76 / 1000 = 0.076 mM; the exported value is 0.076 mM. kcat: printed Table 3 cell 210 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 210 s^(-1). kcat/Km: printed Table 3 cell 2.80 x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 2800000 / 1000 = 2800 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Sulbactam': Km = 76 +/- 4 uM; kcat = 210 s^-1; printed kcat/Km = 2.80 x 10^6 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Sulbactam' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Sulbactam


substrate_name: Cefoxitin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cefoxitin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 2 +/- 0.05; column 'kcat (s-1)' = 1.10; column 'kcat/Km (M-1 s-1)' = 5.50 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 2 uM; 2 / 1000 = 0.002 mM; the exported value is 0.002 mM. kcat: printed Table 3 cell 1.10 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 1.1 s^(-1). kcat/Km: printed Table 3 cell 5.50 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 550000 / 1000 = 550 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Cefoxitin': Km = 2 +/- 0.05 uM; kcat = 1.10 s^-1; printed kcat/Km = 5.50 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cefoxitin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Cefoxitin


substrate_name: Moxalactam

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Moxalactam' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 1 +/- 0.05; column 'kcat (s-1)' = 0.29; column 'kcat/Km (M-1 s-1)' = 2.90 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 1 uM; 1 / 1000 = 0.001 mM; the exported value is 0.001 mM. kcat: printed Table 3 cell 0.29 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.29 s^(-1). kcat/Km: printed Table 3 cell 2.90 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 290000 / 1000 = 290 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Moxalactam': Km = 1 +/- 0.05 uM; kcat = 0.29 s^-1; printed kcat/Km = 2.90 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Moxalactam' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Moxalactam


column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Carbenicillin' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 4200 +/- 300; column 'kcat (s-1)' = 755; column 'kcat/Km (M-1 s-1)' = 1.80 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 4200 uM; 4200 / 1000 = 4.2 mM; the exported value is 4.2 mM. kcat: printed Table 3 cell 755 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 755 s^(-1). kcat/Km: printed Table 3 cell 1.80 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 180000 / 1000 = 180 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Carbenicillin': Km = 4200 +/- 300 uM; kcat = 755 s^-1; printed kcat/Km = 1.80 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Carbenicillin' within the 'Bacillus cereus beta-lactamase II' column group