enzyme_display_name: firefly luciferase

organism: Photinus pyralis

substrate_name: D-luciferin

assay_conditions_summary: pH 8.2; buffer phosphate buffer; cofactor ATP

proof_lines: pH 8.2, temperature not specified in the publication

proof_lines: pH 8.5, temperature not specified in the publication

proof_lines: pH 9.1, temperature not specified in the publication

enzyme: firefly luciferase

substrate: D-luciferin


enzyme_display_name: 4-hydroxyphenylpyruvate dioxygenase

organism: Zea mays

source_db: brenda

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

enzyme: 4-hydroxyphenylpyruvate dioxygenase

source: brenda


organism: Luciola mingrelica

substrate_name: Luciferin

kcat_display: Not reported

assay_conditions_summary: cofactor ATP

proof_lines: 150 microM luciferin, presence of 1 microM of N-quinolin-2-ylbenzamide, two-site binding model

substrate: Luciferin


enzyme_display_name: camphor 5-monooxygenase

organism: Pseudomonas putida

enzyme: camphor 5-monooxygenase


proof_lines: P-450cam, constituent part of the multi-component enzyme

proof_lines: constituent protein of the multi-component oxygenase


organism: Pseudomonas sp. TCU-HL1

substrate_name: reduced putidaredoxin

km_display: Not reported

protein_accession_database: UniProt

proof_lines: redox partner is Pdx, i.e native redox partner for isoform P450cam, pH 7.4, 25°C

proof_lines: redox partner is Pdxtcu, i.e native redox partner for isoform P450tcu, pH 7.4, 25°C

substrate: reduced putidaredoxin


organism: Homo sapiens

substrate_name: testosterone

assay_conditions_summary: pH 7.4; 310.15 K; cofactor NAD+

proof_lines:
pH 7.4, with NAD+ as cofactor
pH 7.4, reaction with estradiol
pH 7.4, reaction with testosterone

substrate: testosterone


organism: Sus scrofa

proof_lines:
microsomal enzyme
cytosolic enzyme
mitochondrial enzyme


enzyme_display_name: glutathione-disulfide reductase

organism: Allochromatium vinosum

substrate_name: NADH

assay_conditions_summary: cofactor NADPH

enzyme: glutathione-disulfide reductase

substrate: NADH


organism: Anabaena sp.

substrate_name: NADPH

substrate: NADPH


organism: Arabidopsis thaliana

assay_conditions_summary: pH 7.4; buffer 100 mM K2HPO4/KH2PO4; cofactor NADPH

proof_lines: in 100 mM K2HPO4/KH2PO4 buffer, at pH 7.4


organism: Bos taurus

assay_conditions_summary: pH 7; 303.15 K; cofactor NADPH

proof_lines:
calf liver enzyme, pH 7.0, 30°C
pH 7.4, 37°C
erythrocyte enzyme, pH 7.0, 30°C


organism: Camelus dromedarius

substrate_name: glutathione disulfide

assay_conditions_summary: pH 7.5; 323.15 K; cofactor NADPH

substrate: glutathione disulfide


organism: Colwellia psychrerythraea

assay_conditions_summary: 298.15 K; cofactor NADPH

proof_lines: 25°C, pH not specified in the publication

proof_lines: 4°C, pH not specified in the publication


organism: Cyberlindnera mrakii


enzyme_display_name: alanine racemase

organism: Acinetobacter baumannii

substrate_name: D-alanine

proof_lines: pH not specified in the publication, 30°C

enzyme: alanine racemase

substrate: D-alanine


organism: Aeromonas hydrophila subsp. hydrophila

substrate_name: Unknown

ki_unit: mM

proof_lines: pH 11.0, 35°C, recombinant His-tagged enzyme

substrate: Unknown


substrate_name: L-alanine

proof_lines: recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication

substrate: L-alanine


organism: Alkalihalophilus pseudofirmus


proof_lines: pH 10.5, 40°C, recombinant wild-type enzyme


organism: Bacillus anthracis


organism: Caldanaerobacter subterraneus subsp. tengcongensis


organism: Clostridioides difficile

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution


organism: Geobacillus stearothermophilus


organism: Peribacillus psychrosaccharolyticus

substrate_name: D-Ala

substrate: D-Ala


organism: Pseudomonas aeruginosa

proof_lines: pH and temperature not specified in the publication


enzyme_display_name: arylacetonitrilase

organism: Alcaligenes sp.

substrate_name: mandelonitrile

proof_lines:
at pH 6.5 and 50°C
at pH 6.5 and 30°C

enzyme: arylacetonitrilase

substrate: mandelonitrile


organism: Aspergillus niger

substrate_name: phenylpropionitrile

substrate: phenylpropionitrile


organism: uncultured bacterium

substrate_name: phenylacetonitrile

substrate: phenylacetonitrile


enzyme_display_name: inorganic diphosphatase

organism: Anabaena sp.

substrate_name: Diphosphate; H2O

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

enzyme: inorganic diphosphatase

substrate: Diphosphate; H2O


organism: Arabidopsis thaliana

substrate_name: H2O; Diphosphate

substrate: H2O; Diphosphate


organism: Synechocystis sp.


organism: Vigna radiata

substrate_name: Bis-Tris-propane diphosphate; H2O

substrate: Bis-Tris-propane diphosphate; H2O


organism: Pyrococcus horikoshii


organism: Salmonella enteritidis PT4 P125109


organism: Schizosaccharomyces pombe


organism: Shigella dysenteriae

substrate_name: L-Ala

substrate: L-Ala


organism: Staphylococcus aureus

proof_lines: pH not specified in the publication, 30°C, recombinant enzyme


organism: Streptococcus iniae


organism: Streptococcus pneumoniae


organism: Alcaligenes faecalis


organism: Hemicentrotus pulcherrimus


organism: Homo sapiens

substrate_name: (Z)-ajoene

assay_conditions_summary: pH 7.5; 298.15 K; buffer 100 mM Tris-HCl; cofactor NADPH

proof_lines:
25°C
pH 7.5
reoxidation

substrate: (Z)-ajoene


organism: Ipomoea batatas

assay_conditions_summary: pH 8; 298.15 K; buffer 100 mM potassium phosphate; cofactor NADPH

proof_lines: in 100 mM potassium phosphate buffer (pH 8.0), at 25°C


organism: Lathyrus oleraceus

substrate_name: GSSG

assay_conditions_summary: cofactor NADP+

proof_lines:
root enzyme
root and chloroplast enzyme
chloroplast enzyme

substrate: GSSG


organism: Limnospira maxima

substrate_name: mixed disulfide of CoA and glutathione

assay_conditions_summary: cofactor CoA

substrate: mixed disulfide of CoA and glutathione


organism: Lithobates sylvaticus

proof_lines: enzyme from unfrozen state, presence of glucose, pH 7.2, 25°C

proof_lines: enzyme from unfrozen state, presence of urea, pH 7.2, 25°C


organism: Litomosoides carinii


organism: Moniezia expansa

substrate_name: NADH

assay_conditions_summary: cofactor NADH

substrate: NADH


organism: Oncorhynchus mykiss

assay_conditions_summary: pH 8; 298.15 K; cofactor NADPH


organism: Ovis aries

substrate_name: NADPH

substrate: NADPH


organism: Phaeodactylum tricornutum

substrate_name: glutathione disulfide

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADPH

proof_lines: non-competitive product inhibition regarding NADPH, at 30°C and pH 7.5

proof_lines: competitive product inhibition regarding glutathione disulfide, at 30°C and pH 7.5

substrate: glutathione disulfide


organism: Plasmodium falciparum


proof_lines: kcat_Km above 0.8 1/sec*mM, pH 7.0, 0.1 M potassium phosphate, 1 mM EDTA, 25°Ckcat above 0.1 1/sec, pH 7.0, 0.1 M potassium phosphate, 1 mM EDTA, 25°C


organism: Psychrobacter sp. ANT206

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH


organism: Pteris ensiformis

assay_conditions_summary: pH 7; buffer 50 mM potassium phosphate; cofactor NADPH

proof_lines: in 50 mM potassium phosphate buffer (pH 7.0)


organism: Pteris vittata

assay_conditions_summary: pH 7; buffer note; cofactor NADPH


organism: Rattus norvegicus

assay_conditions_summary: pH 7.4; 298.15 K; buffer 100 mM potassium phosphate; cofactor NADPH

proof_lines: 25°C, pH not specified in the publication

proof_lines: in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C


organism: Saccharomyces cerevisiae

substrate_name: S-allylmercaptoglutathione disulfide

proof_lines: at 25°C, pH not specified in the publication

substrate: S-allylmercaptoglutathione disulfide


organism: Scombridae

substrate_name: Unknown

substrate: Unknown


organism: Spinacia oleracea


organism: Sus scrofa


organism: Taenia crassiceps

assay_conditions_summary: pH 7.8; 298.15 K; cofactor NADPH


organism: Trachemys scripta

assay_conditions_summary: pH 7.4; cofactor NADPH

proof_lines: anoxic enzyme in 50 mM potassium phosphate, pH 7.4, 1 mM EDTA, 0.1 mM GSSG and 0.2 mM NADPH


organism: Triticum aestivum

assay_conditions_summary: cofactor NADPH


organism: Triticum turgidum subsp. durum


enzyme_display_name: thymidylate synthase

organism: Angiostrongylus cantonensis

proof_lines: 0.25 mM, noncompetitive inhibition, 5,10-mehylenetetrahydrofolate as variable substrate

proof_lines: 1.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate

enzyme: thymidylate synthase


organism: Bacillus subtilis


organism: Bos taurus


organism: Enterobacteria phage T6


organism: Escherichia coli


organism: Escherichia phage T2


organism: Gallus gallus


organism: Homo sapiens

substrate_name: dUMP

proof_lines: apparent value, mutant enzyme V3A, pH not specified in the publication, at 37°C

substrate: dUMP


proof_lines: mutant enzyme A191K, in 50 mM Tris-HCl, pH 7.4, 1 mM EDTA, 0.2% 2-mercaptoethanol, at 22°C


proof_lines: uncompetitive with methylene tetrahydrofolate


organism: Lacticaseibacillus casei


organism: Kitasatospora aureofaciens


organism: Plasmodium berghei


organism: Mycobacterium tuberculosis


organism: Mus musculus

substrate_name: methylenetetrahydrofolate

proof_lines:
Ehrlich ascites carcinoma cells
L1210 recombinant enzyme
L1210 parental enzyme

substrate: methylenetetrahydrofolate


organism: Saccharomyces cerevisiae


organism: Streptococcus pneumoniae


organism: Rattus norvegicus


organism: Plasmodium falciparum

proof_lines:
recombinant enzyme
native enzyme


enzyme_display_name: tyramine N-feruloyltransferase

organism: Nicotiana tabacum

proof_lines:
+ tyramine
+ feruloyl-CoA
+ feruloyl-CoA, dopamine + feruloyl-CoA

enzyme: tyramine N-feruloyltransferase


organism: Capsicum annuum

substrate_name: feruloyl-CoA

assay_conditions_summary: pH 8.5; 303.15 K; cofactor CoA

substrate: feruloyl-CoA


organism: Zea mays

substrate_name: tyramine

proof_lines:
with feruloyl-CoA as acceptor
with tyramine as acceptor

substrate: tyramine


organism: Papaver somniferum


enzyme_display_name: carnitine O-palmitoyltransferase

organism: Bos taurus

substrate_name: L-carnitine

proof_lines:
peroxisomal soluble enzyme
with C8-CoA
inner mitochondrial membrane

enzyme: carnitine O-palmitoyltransferase

substrate: L-carnitine


organism: Solanum tuberosum


organism: Ctenopharyngodon idella

proof_lines: enzyme from 6 month old animal, at 25°C, pH not specified in the publication

proof_lines: enzyme from 1 month old animal, at 25°C, pH not specified in the publication

proof_lines: enzyme at hatching, at 25°C, pH not specified in the publication


organism: Felis catus

proof_lines:
pH 7.2, 30°C, liver CPT 1
pH 7.2, 30°C, skeletal muscle CPT 1


organism: Gallus gallus

substrate_name: butyryl-CoA

substrate: butyryl-CoA


organism: Drosophila melanogaster

substrate_name: palmitoyl-CoA

proof_lines: KI-values: 0.00064 mM and 0.0007 mM malony-CoA (from secondary Dixon plots)

substrate: palmitoyl-CoA


organism: Homo sapiens

assay_conditions_summary: 303.15 K; cofactor CoA


assay_conditions_summary: pH 8; 310.15 K; cofactor CoA

proof_lines: pH 8.0, 37°C, recombinant revertant A305C


proof_lines: D17E mutant protein, mitochondrial preparation


organism: Macrobrachium borellii


substrate_name: stearoyl-CoA

substrate: stearoyl-CoA


proof_lines:
recombinant wild-type L-CPT I
recombinant wild-type CPT I


assay_conditions_summary: pH 7.4; 303.15 K; cofactor CoA

proof_lines: isoform L-CPT I, with 2[6-(4-chlorophenoxy)hexyl]oxirane-2-carboxylic acid

proof_lines: purified recombinant L-CPT I, reconstituted in liposomes


organism: Sus scrofa

assay_conditions_summary: pH 7.2; 303.15 K; cofactor CoA

proof_lines:
3 wk old pig, liver
5 wk old pig, liver
adult pig, skeletal muscle


proof_lines: wild-type protein, mitochondrial preparation


proof_lines: E17D mutant protein, mitochondrial preparation


enzyme_display_name: arylamine N-acetyltransferase

substrate_name: Phenylethylamine

proof_lines: liver brain

enzyme: arylamine N-acetyltransferase

substrate: Phenylethylamine


organism: Bacillus cereus

proof_lines:
Vmax: 2.1 micromol/min/mg
Vmax: 0.17 micromol/min/mg


organism: Oryctolagus cuniculus


organism: Mycobacterium marinum

substrate_name: isoniazid

assay_conditions_summary: pH 8; 301.15 K; cofactor CoA

substrate: isoniazid


enzyme_display_name: aralkylamine N-acetyltransferase

substrate_name: acetyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: aralkylamine N-acetyltransferase

substrate: acetyl-CoA


organism: Arabidopsis thaliana


organism: Aedes aegypti

substrate_name: dopamine

proof_lines: isoform aaNAT2, pH not specified in the publication, temperature not specified in the publication

substrate: dopamine


organism: Ovis aries

assay_conditions_summary: pH 6.8; 303.15 K; cofactor CoA

proof_lines: pH 6.8, 30°C, phosphorylated T31, unphosphorylated S205


organism: Onchocerca volvulus


substrate_name: serotonin

proof_lines:
wild-type enzyme
wild-type enzyme with 14-3-3 protein
wild-type enzyme with protein kinase A

substrate: serotonin


organism: Pyropia yezoensis


organism: Rattus norvegicus

proof_lines:
cosubstrate tryptamine, pineal gland
cosubstrate tryptamine, liver
pineal gland


organism: Saccharopolyspora erythraea

assay_conditions_summary: pH 7; 298.15 K; cofactor CoA

proof_lines:
cosubstrate acetyl-CoA, pH 7.0, 25°C
cosubstrate octopamine, pH 7.0, 25°C


organism: Pelophylax perezi


enzyme_display_name: pyrimidine-nucleoside phosphorylase

organism: Escherichia coli

proof_lines: pH 7.5, 25°C, thymidine phosphorylase activity

proof_lines: pH 7.5, 25°C, uridine phosphorylase activity

enzyme: pyrimidine-nucleoside phosphorylase


organism: Bacillus subtilis

proof_lines: pH and temperature not specified in the publication


organism: Geobacillus stearothermophilus

substrate_name: uridine

substrate: uridine


organism: Haemophilus influenzae


organism: Parageobacillus thermoglucosidasius

substrate_name: thymidine

proof_lines:
pH 7.0, 60°C
Km about 1 mM, at pH 7.0 and 40°C
at pH 7.0 and 40°C

substrate: thymidine


organism: Mesomycoplasma hyorhinis

substrate_name: phosphate

proof_lines:
cosubstrate uridine, pH 7.6, 37°C
at pH 7.6 and 37°C
pH 7.6, 37°C

substrate: phosphate


enzyme_display_name: corrinoid adenosyltransferase

organism: Homo sapiens

substrate_name: cob(I)alamin

assay_conditions_summary: 239.00 K; cofactor ATP

proof_lines:
recombinant enzyme variant 239K
recombinant enzyme variant 239M

enzyme: corrinoid adenosyltransferase

substrate: cob(I)alamin


organism: Limosilactobacillus reuteri

substrate_name: ATP

assay_conditions_summary: cofactor ATP

substrate: ATP


organism: Lactobacillus leichmannii

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP


organism: Clostridium tetanomorphum

substrate_name: cyanocob(I)alamin

proof_lines: pH 8.0, 37°C, i.e. vitamin B12, reduced form

substrate: cyanocob(I)alamin


organism: Methanosarcina mazei


organism: Serratia plymuthica


organism: Salmonella enterica subsp. enterica serovar Typhimurium


organism: Salmonella enterica

assay_conditions_summary: pH 7; 310.15 K; cofactor ATP

proof_lines:
pH 7.0, 37°C, recombinant enzyme
at pH 7.0 and 37°C


enzyme_display_name: cysteine synthase

organism: Brassica juncea

substrate_name: O-acetyl-L-Ser

enzyme: cysteine synthase

substrate: O-acetyl-L-Ser


organism: Aeropyrum pernix

substrate_name: thiosulfate

proof_lines:
synthesis of S-sulfo-L-cysteine
sulfhydrylation of O-acetyl-L-serine

substrate: thiosulfate


organism: Arabidopsis thaliana

proof_lines: pH 7.5, 25°C, recombinant complex-bound enzyme

proof_lines: isoform B, pH 7.5, 25°C, Michaelis-Menten kinetics


organism: Enterobacter sp.

substrate_name: NaCN

proof_lines:
pH 7.4, 30°C
pH 7.4, 30°C, cosubstrate-dependent

substrate: NaCN


organism: Capsicum annuum


organism: Escherichia coli

proof_lines: pH 7.5, 25°C, enzyme bound to serine acetyltransferase


organism: Corynebacterium glutamicum


organism: Hordeum vulgare


organism: Fusobacterium nucleatum subsp. nucleatum

substrate_name: O-acetyl-L-serine

substrate: O-acetyl-L-serine


organism: Geobacillus stearothermophilus


organism: Cavia porcellus

substrate_name: testosterone

assay_conditions_summary: cofactor NADP+

substrate: testosterone


organism: [Clostridium] innocuum


organism: Comamonas testosteroni

substrate_name: 3-pyridinealdehyde adenine dinucleotide

assay_conditions_summary: cofactor NAD+

substrate: 3-pyridinealdehyde adenine dinucleotide


organism: Rattus norvegicus

proof_lines: 3beta-hydroxy-5alpha-androstan-17-one as substrate, 3beta-hydroxysteroid dehydrogenase activity

proof_lines: 5alpha-dihydrotestosterone as substrate, 3-beta-hydroxysteroid dehydrogenase activity

proof_lines: estrone as substrate, 17beta-hydroxysteroid dehydrogenase activity


organism: Paracoccus denitrificans


organism: Penicillium chrysogenum


organism: Raphanus sativus


organism: Saccharomyces cerevisiae

substrate_name: O-acetylhomoserine

substrate: O-acetylhomoserine


organism: Lathyrus sativus


organism: Leishmania major

substrate_name: Unknown

substrate: Unknown


organism: Leucaena leucocephala

substrate_name: hydrogen sulfide

substrate: hydrogen sulfide


organism: Methanosarcina thermophila


organism: Salmonella enterica subsp. enterica serovar Typhimurium

proof_lines:
25°C, 100 mM HEPES, pH 7.0
less than
complex-bound enzyme


organism: Solanum tuberosum

substrate_name: CN-

proof_lines:
pH 8.5, enzyme form PCS-2
pH 7.5, enzyme form PCS-1
pH 8.5, enzyme form PCS-1

substrate: CN-


organism: Thermus thermophilus


organism: Trichomonas vaginalis

proof_lines: 37°C, pH 7.8, cosubstrate: O-phosphoserine

proof_lines: 37°C, pH 7.8, cosubstrate: O-acetyl-L-Ser


organism: Trifolium repens


organism: Triticum aestivum


enzyme_display_name: polo kinase

organism: Homo sapiens

proof_lines: Ki, apparent dissociation constant, less than 0.0000005

enzyme: polo kinase


substrate_name: casein

substrate: casein


enzyme_display_name: kynurenine--oxoglutarate transaminase

organism: Penaeus monodon

substrate_name: L-kynurenine

enzyme: kynurenine--oxoglutarate transaminase

substrate: L-kynurenine


organism: Rattus norvegicus

proof_lines: pH 8.0, 37°C, recombinant enzyme, cosubstrate 2-oxoglutarate


organism: Hansenula schneggii

proof_lines:
pH 8.0, 37°C, cosubstrate L-kynurenine
pH 8.0, 37°C
pH 8.0. 25°C


proof_lines:
discontinuous assay, pH 7.5, 45°C
pH 7.5, 45°C
continuous assay, pH 7.5, 45°C


organism: Mus musculus

substrate_name: phenylpyruvate

substrate: phenylpyruvate


organism: Neurospora crassa

substrate_name: pyruvate

proof_lines:
pH 7.5, 37°C, cosubstrate L-kynurenine
pH 7.5, 37°C

substrate: pyruvate


organism: Bos taurus

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP


organism: Escherichia coli

substrate_name: deamido-NAD+

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NAD+

substrate: deamido-NAD+


substrate_name: nicotinamide ribonucleotide

assay_conditions_summary: pH 7.6; 310.15 K; cofactor NAD+

substrate: nicotinamide ribonucleotide


organism: Katsuwonus pelamis

substrate_name: NAD+

substrate: NAD+


enzyme_display_name: N-acetylgalactosamine kinase

substrate_name: ATP

assay_conditions_summary: pH 7; 298.15 K; cofactor ATP

proof_lines:
recombinant enzyme
pH 7.0, 25°C

enzyme: N-acetylgalactosamine kinase

substrate: ATP


organism: Sus scrofa


organism: Leishmania braziliensis

assay_conditions_summary: pH 7.4; 293.15 K; cofactor NAD+


organism: Pyrococcus horikoshii

assay_conditions_summary: pH 7; 343.15 K; cofactor ATP


organism: Saccharolobus solfataricus

substrate_name: NMN

assay_conditions_summary: pH 7.4; 343.15 K; cofactor ATP

substrate: NMN


organism: Saccharomyces cerevisiae


assay_conditions_summary: pH 10; 298.15 K; cofactor ATP

proof_lines:
25°C, pH 10
25°C, pH 5.15
immobilized enzyme


organism: Acanthamoeba castellanii

substrate_name: alpha-D-glucose 1-phosphate

substrate: alpha-D-glucose 1-phosphate


substrate_name: diphosphate

substrate: diphosphate


organism: Cucumis sativus

substrate_name: UTP

proof_lines:
pH 7.2, 30°C
diphosphate in form of Mg-diphosphate

substrate: UTP


substrate_name: TTP

substrate: TTP


organism: Ganoderma lucidum

substrate_name: UDP-glucose

proof_lines: pH 7.5, temperature not specified in the publication

substrate: UDP-glucose


organism: Homo sapiens

proof_lines: non-galactosemic phenotype, pH 7.8, 37°C


organism: Hordeum vulgare


organism: Hordeum vulgare subsp. vulgare


organism: Lilium longiflorum


organism: Oryctolagus cuniculus


organism: Pseudomonas aeruginosa


assay_conditions_summary: pH 7.6; 310.15 K; cofactor ATP

proof_lines:
pH 7.6, 37°C
pH 7.6, 70°C
cosubstrate UTP, pH 7.6, 37°C


substrate_name: methylenebisphosphonate

substrate: methylenebisphosphonate


organism: Solanum tuberosum

proof_lines: at physiological substrate concentration, pH 8.5, 37°C

proof_lines: at physiological substrate concentrations, pH 8.5, 37°C


organism: Sorghum bicolor


organism: Streptococcus pneumoniae


organism: Triticum aestivum

assay_conditions_summary: pH 9

proof_lines: pH 9.0, temperature not specified in the publication


enzyme_display_name: aryl sulfotransferase

organism: Drosophila melanogaster

substrate_name: 3'-phosphoadenylyl sulfate

proof_lines: isoform ST4, in 50 mM sodium phosphate, pH 6.8, at 37°C

proof_lines: isoform ST1, in 50 mM sodium phosphate, pH 6.8, at 37°C

proof_lines: isoform ST3, in 50 mM sodium phosphate, pH 6.8, at 37°C

enzyme: aryl sulfotransferase

substrate: 3'-phosphoadenylyl sulfate


organism: Euglena gracilis


organism: Haliangium ochraceum


organism: Ictalurus punctatus

proof_lines:
intestinal enzyme, pH 7.0, 35°C
liver enzyme, pH 7.0, 35°C


organism: Mus musculus

substrate_name: p-nitrophenol

assay_conditions_summary: pH 7.4; 310.15 K; buffer 50 mM Tris/HCl

proof_lines: isoform Sult1a1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C

substrate: p-nitrophenol


organism: Arachis hypogaea

proof_lines:
enzyme from plasma membrane
enzyme from Golgi apparatus


organism: Cajanus cajan

proof_lines:
enzyme N-I
enzyme N-I, enzyme N-II
enzyme N-II


organism: Cavia porcellus


organism: Columba livia

assay_conditions_summary: pH 7; 310.15 K; cofactor ADP

proof_lines:
37°C, pH 7.0, presence of 1 mM ADP
37°C, pH 7.0


organism: Crocodylus siamensis

substrate_name: 5'-inosinic acid

substrate: 5'-inosinic acid


organism: Danio rerio

substrate_name: MgAMP

substrate: MgAMP


organism: Dosidicus gigas

substrate_name: AMP

assay_conditions_summary: 323.15 K; buffer note

proof_lines: in 40 mM sodium citrate buffer, 0.1 mM dithiothreitol, 110 mM NaCl, 20 mM MgCl2, and 20 mM CaCl2, at 50°C

substrate: AMP


organism: Gadus macrocephalus


organism: Salvator rufescens


organism: Sebastes inermis

proof_lines:
membrane-bound enzyme
cytosolic enzyme


organism: Streptomyces sp. NE5-10


organism: Streptomyces sp. Y2F8-2


organism: Gallus gallus

assay_conditions_summary: cofactor ATP


organism: Homo sapiens

assay_conditions_summary: pH 8.5; 298.15 K; cofactor ATP

proof_lines:
presence of 3 mM ATP
at pH 8.5
pH 8.3, 25°C


organism: Metamycoplasma arthritidis

verification_status: unverified

status: unverified


proof_lines: pH 7.4, 37°C, enzyme from dystrophic muscle


organism: Mycoplasmopsis fermentans

proof_lines: pH 8.5, 37°C, strain KL8, strain BRO and strain M106


organism: Mycoplasmopsis pulmonis


organism: Neurospora crassa


organism: Oncorhynchus masou


organism: Oryctolagus cuniculus


organism: Rattus norvegicus

proof_lines:
synaptic membrane-bound
brain cytosol
enzyme from glioblastoma cells


organism: Rhipicephalus microplus

substrate_name: p-Nitrophenyl phenylphosphonate

substrate: p-Nitrophenyl phenylphosphonate


organism: Saccharomyces cerevisiae

proof_lines: 5-nucleotidase Sdt1, pH not specified in the publication, temperature not specified in the publication


organism: Tritrichomonas suis


organism: Varanus gouldii


organism: Zea mays


organism: Sus scrofa


organism: Torpedo marmorata


organism: Trichomonas gallinae

assay_conditions_summary: pH 7.2; 310.15 K; buffer 50 mM Tris

proof_lines: in 50 mM Tris buffer (pH 7.2) and 3.0 mM MgCl2, at 37°C


organism: Trichomonas vaginalis


organism: Bifidobacterium bifidum


organism: Elizabethkingia meningoseptica

substrate_name: 4-nitrophenyl alpha-L-fucoside

substrate: 4-nitrophenyl alpha-L-fucoside


organism: Prunus dulcis

substrate_name: lacto-N-fucopentaitol III

substrate: lacto-N-fucopentaitol III


enzyme_display_name: chitinase

organism: Aeromonas media

substrate_name: chitopentaose

enzyme: chitinase

substrate: chitopentaose


organism: Annona cherimola

substrate_name: 4-methylumbelliferyl chitobioside

substrate: 4-methylumbelliferyl chitobioside


organism: Autographa californica


organism: Bacillus cereus

mutation_type: truncation

proof_lines: 37°C, C-terminal truncated enzyme ChiCWDELTAFC


organism: Bacillus licheniformis

substrate_name: colloidal chitin

substrate: colloidal chitin


organism: Bacillus sp. (in: firmicutes)

substrate_name: chitin

substrate: chitin


organism: Caldicellulosiruptor acetigenus


organism: Coccidioides immitis

substrate_name: chitohexaose

substrate: chitohexaose


organism: Coprinopsis cinerea


organism: Ficus pumila var. awkeotsang


organism: Homo sapiens


organism: Hydrogenophilus hirschii


organism: Leishmania major


organism: Metarhizium anisopliae


organism: Nepenthes alata

substrate_name: ethlene glycol chitin

substrate: ethlene glycol chitin


organism: Niallia circulans


organism: Oryza sativa


organism: Paenibacillus sp.


organism: Paenibacillus thermoaerophilus


organism: Paenibacillus timonensis


organism: Penicillium ochrochloron


organism: Penicillium oxalicum


organism: Pennahia argentata

substrate_name: glycol chitin

substrate: glycol chitin


organism: Saccharomyces cerevisiae

proof_lines: expressed in N-acetylglucosamine equivalents


organism: Scorpaena scrofa


organism: Serratia marcescens


organism: Setaria cervi


organism: Stenotrophomonas maltophilia


organism: Stomoxys calcitrans


organism: Streptomyces chilikensis

proof_lines: 37°C, pH not specified in the publication


organism: Streptomyces sp.

proof_lines: Chi-A Chi-B


organism: Tenebrio molitor


organism: Anabaena sp.

substrate_name: diphosphate

source_db: oed

substrate: diphosphate

source: oed


organism: Arabidopsis thaliana


substrate_name: H2O; Diphosphate

substrate: H2O; Diphosphate


proof_lines: pH 7.5, 22°C, recombinant truncated mutant AtPPA1-DELTA(1-29)


proof_lines: pH 7.5, 22°C, recombinant wild-type enzyme


proof_lines: pH 7.5, 25°C, isozyme PPa4 in a complex with Mg2+


organism: Bacillus subtilis

substrate_name: imidodiphosphoric acid

proof_lines: pH 7.2, 25°C, wild-type enzyme, kinetics

substrate: imidodiphosphoric acid


source_db: skid

source_license: CC BY-NC-ND 4.0

source: skid


organism: Bacteroides thetaiotaomicron


proof_lines: pH 7.5, 25°C, recombinant wild-type BT2127


organism: Chlamydomonas reinhardtii

substrate_name: Diphosphate; H2O

substrate: Diphosphate; H2O


organism: Clostridium perfringens


organism: Cyanidium caldarium


organism: Cyanophora paradoxa


organism: Desulfitobacterium hafniense


assay_conditions_summary: pH 7.2; 310.15 K; cofactor ATP

proof_lines: pH 7.2, 37°C, mutant R276E, in presence of 0.5 mM AMP

proof_lines: pH 7.2, 37°C, mutant R276E, in presence of 0.5 mM ATP

proof_lines: pH 7.2, 37°C, mutant R276E, in presence of 0.5 mM ADP


proof_lines: pH 7.2, 37°C, mutant R276K, in presence of 0.5 mM ATP

proof_lines: pH 7.2, 37°C, mutant R276K, in presence of 0.5 mM Ap4A


proof_lines: pH 7.2, 37°C, mutant R276A, in presence of 0.5 mM ATP

proof_lines: pH 7.2, 37°C, mutant R276A, in presence of 0.5 mM AMP

proof_lines: pH 7.2, 37°C, mutant R276A, in presence of 0.5 mM Ap4A


proof_lines: pH 7.2, 37°C, wild-type enzyme, in presence of 0.5 mM AMP

proof_lines: pH 7.2, 37°C, wild-type enzyme, in presence of 0.5 mM ADP

proof_lines: pH 7.2, 37°C, wild-type enzyme, in presence of 0.5 mM Ap4A


organism: Escherichia coli

substrate_name: Unknown

proof_lines:
pH 7.4, 25°C, with 0.05 mM Mn2+
pH 7.4, 25°C, with 5 mM Mn2+
recombinant enzyme, pH 7.5, 37°C

substrate: Unknown


organism: Ethanoligenens harbinense


organism: Euglena gracilis


organism: Haloferax volcanii

proof_lines:
recombinant enzyme, pH 8.5, 42°C
recombinant enzyme, pH 8.5, 25°C


organism: Helicobacter pylori

source_license: CC BY 4.0; CC BY-NC-ND 4.0

proof_lines:
recombinant enzyme
Raw SKiD Env: Temp=37°C, pH=8
Raw SKiD Env: Temp=-----, pH=-----

source_databases_merged: brenda skid


proof_lines: pH 8.0, 37°C, recombinant wild-type enzyme


organism: Homo sapiens

substrate_name: cAMP; H2O

substrate: cAMP; H2O


organism: Legionella pneumophila subsp. pneumophila

substrate_name: 4-nitrophenyl phosphate

proof_lines: pH 7.2, temperature not specified in the publication

substrate: 4-nitrophenyl phosphate


organism: Leishmania major

proof_lines:
pH 7.5, in the presence of Ca2+
pH 7.5, in the presence of Mg2+


organism: Medicago truncatula


proof_lines: pH 7.5, 22°C, recombinant wild-type enzyme


organism: Methanothrix thermoacetophila


proof_lines: pH 8.0, 37°C, with Mn2+ as metal cofactor


organism: Mycobacterium tuberculosis


proof_lines: pH 7.5, 20°C, mutant enzyme Ec-Mt-PPase with the missing part of the polypeptide chain (N-terminal region) compensated with a fragment of PPase from Escherichia coli


organism: Ochromonas danica


organism: Plasmodium falciparum


substrate_name: ATP

substrate: ATP


proof_lines: pH 7.2, 37°C, recombinant wild-type enzyme


organism: Pseudanabaena sp.


organism: Pyrococcus furiosus

substrate_name: UDP-glucose

substrate: UDP-glucose


substrate_name: ADP

assay_conditions_summary: pH 9.5; 368.15 K; cofactor ADP

substrate: ADP


substrate_name: UDP

substrate: UDP


substrate_name: IDP

substrate: IDP


organism: Rhodopseudomonas viridis


organism: Rhodospirillum rubrum


organism: Saccharolobus islandicus


organism: Streptococcus gordonii


proof_lines: pH 9.0, 25°C, 5 mM Mg2+, wild-type enzyme

proof_lines: recombinant wild-type enzyme, pH 9.0, 25°C


substrate_name: ITP

substrate: ITP


substrate_name: Triphosphate

substrate: Triphosphate


assay_conditions_summary: pH 9.5; 368.15 K; cofactor ATP


substrate_name: CTP

substrate: CTP


substrate_name: GDP

substrate: GDP


substrate_name: CDP

substrate: CDP


substrate_name: GTP

substrate: GTP


substrate_name: UTP

substrate: UTP


substrate_name: diphosphate

substrate: diphosphate


organism: Pyrococcus horikoshii

proof_lines:
pH 7.5, 60°C, recombinat enzyme
pH 8.0, 90°C


organism: Rhipicephalus microplus


organism: Synechocystis sp.

proof_lines:
recombinant enzyme
native enzyme


organism: Thermococcus onnurineus


proof_lines: wild type enzyme, in 50 mM MOPS (pH 6.5), in the presence of Co2+, at 80°C

proof_lines: wild type enzyme, in 50 mM MOPS (pH 6.5), in the presence of Ni2+, at 80°C

proof_lines: wild type enzyme, in 50 mM MOPS (pH 6.5), in the presence of Mg2+, at 80°C


proof_lines: pH and temperature not specified in the publication


organism: Thermoplasma acidophilum


proof_lines: recombinant mutants I260V and I260E, pH 9.0, 25°C


organism: Sulfolobus acidocaldarius

assay_conditions_summary: pH 3; 328.15 K; cofactor ATP

proof_lines:
pH 3.0, 55°C
Raw SKiD Env: Temp=55°C, pH=3


organism: Arabidopsis thaliana

substrate_name: PPi

source_db: uniprot

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

data_origin: experimental

substrate: PPi

source: uniprot


substrate_name: pyrophosphate PPi (at 37 degrees Celsius)


substrate_name: pyrophosphate

substrate: pyrophosphate


organism: Bacillus subtilis (strain 168)


organism: Blastochloris viridis

sequence: MRIDAIDXA


organism: Chlamydomonas reinhardtii


organism: Clostridium perfringens (strain 13 / Type A)


organism: Cyanophora paradoxa

sequence: XAITAEPVGTPETLEYRVFIQKDGK


organism: Euglena gracilis

sequence: MDTKLGLVEEEAAN


organism: Methanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT)

substrate_name: diphosphate (with manganese as cofactor)


organism: Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


organism: Ochromonas danica

sequence: XYAFGINGTXRI


organism: Pseudanabaena sp. (strain PCC 6903)


organism: Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)


organism: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

substrate_name: CTP (at pH 8 and at 70 degrees Celsius)

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

assay_conditions_summary: pH 8; cofactor ATP

substrate: CTP (at pH 8 and at 70 degrees Celsius)


organism: Vigna radiata


substrate_name: Unknown

substrate: Unknown


organism: Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)

substrate_name: diphosphate (at 42 degrees Celsius)

proof_lines: Displays non-Michaelis-Menten kinetics for PPi hydrolysis. Hill coefficients indicate cooperative binding to PPi and Mg(2+).

substrate: diphosphate (at 42 degrees Celsius)


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: diphosphate (at pH 7.2)

substrate: diphosphate (at pH 7.2)


organism: Escherichia coli

proof_lines:
pH 7.5, 25°C, mutant K112Q
pH 7.5, 25°C, trimeric mutant K112Q


proof_lines: pH 6.5, wild type, resynthesis of diphosphate

proof_lines: pH 8, wild type, hydrolysis of diphosphate


assay_conditions_summary: pH 8

proof_lines: pH 7.2, D97E, resynthesis of diphosphate


substrate_name: Mg-diphosphate

proof_lines:
pH 7.2, H140Q enzyme, trimeric
pH 7.2, H140Q enzyme, hexameric
pH 8, H140Q enzyme, trimeric

substrate: Mg-diphosphate


proof_lines:
pH 8, hexameric, H136Q enzyme
pH 7.2, hexameric, H136Q enzyme
pH 7.2, H136Q enzyme, trimeric


organism: Thermococcus chitonophagus

substrate_name: p-nitrophenol-beta-D-galactopyranoside

substrate: p-nitrophenol-beta-D-galactopyranoside


organism: Trichoderma atroviride

substrate_name: colloidal chitin

substrate: colloidal chitin


organism: Trichoderma harzianum


organism: Triticum aestivum

substrate_name: chitin

proof_lines: chitin concentration calculated as N-acetylglucosamine

substrate: chitin


enzyme_display_name: limit dextrinase

organism: Sorghum bicolor

substrate_name: pullulan

enzyme: limit dextrinase

substrate: pullulan


organism: Vicia faba


enzyme_display_name: pyroglutamyl-peptidase II

organism: Bos taurus

enzyme: pyroglutamyl-peptidase II


organism: Cavia porcellus

substrate_name: Thyroliberin

substrate: Thyroliberin


organism: Rattus norvegicus


organism: Avena sativa


organism: Hordeum vulgare


organism: Lathyrus oleraceus

substrate_name: amylopectin beta-limit dextrin

substrate: amylopectin beta-limit dextrin


organism: Oryza sativa


organism: Vibrio alginolyticus


enzyme_display_name: coagulation factor XIIa

organism: Homo sapiens

proof_lines:
0.05 M Tris-HCl, pH 7.8, 0.117 M NaCl
0.05 M Tris-HCl, pH 8.0

enzyme: coagulation factor XIIa


organism: Sus scrofa

substrate_name: 5-oxoprolyl 2-naphthylamide

proof_lines: pH 7.5, 37°C, hydrolysis of pyroglutamyl-His-Pro-7-amido-4-methylcoumarin

substrate: 5-oxoprolyl 2-naphthylamide


enzyme_display_name: hepsin

proof_lines:
peptide sequence from PAR-2
peptide sequence from matriptase
peptide sequence from filaggrin

enzyme: hepsin


organism: Mus musculus


enzyme_display_name: subtilisin

organism: Bacillus amyloliquefaciens

substrate_name: MeO-succinyl-Ala-Ala-Phe 4-nitroanilide

proof_lines: succinyl-Ala-Ala-Pro-Phe 4-nitroanilide, subtilisin BPN

enzyme: subtilisin

substrate: MeO-succinyl-Ala-Ala-Phe 4-nitroanilide


organism: Bacillus intermedius


organism: Bacillus licheniformis

substrate_name: Z-Ala-Ala-Leu-p-nitroanilide

proof_lines:
in 20% DMF
in 2% DMF
in 20% DMSO

substrate: Z-Ala-Ala-Leu-p-nitroanilide


organism: Thermococcus kodakarensis

assay_conditions_summary: pH 7.5; 293.15 K; buffer 50 mM Tris-HCl

proof_lines: subtilisin-like serine protease, at 20°C, in 50 mM Tris-HCl buffer, pH 7.5

proof_lines: subtilisin-like serine protease, at 80°C, in 50 mM Tris-HCl buffer, pH 7.5

proof_lines: subtilisin, at 20°C, in 50 mM Tris-HCl buffer, pH 8.0


organism: Bacillus sp. (in: firmicutes)

substrate_name: Succinyl-Ala-Ala-Ala 4-nitroanilide

proof_lines:
subtilisin Carlsberg
subtilisin Sendai
subtilisin DY

substrate: Succinyl-Ala-Ala-Ala 4-nitroanilide


organism: Bacillus subtilis

substrate_name: acetyl-L-Tyr

proof_lines:
30°C, pH 8.0
in 20% DMSO
in 2% DMSO

substrate: acetyl-L-Tyr


organism: Dichelobacter nodosus


organism: Fusarium equiseti


enzyme_display_name: HIV-2 retropepsin

organism: Human immunodeficiency virus 2

substrate_name: IPFAAAQQRK

enzyme: HIV-2 retropepsin

substrate: IPFAAAQQRK


enzyme_display_name: scytalidoglutamic peptidase

organism: Scytalidium lignicola

proof_lines: pH and temperature not specified in the publication

enzyme: scytalidoglutamic peptidase


organism: Melghiribacillus thermohalophilus

substrate_name: azocasein

proof_lines:
pH 10.0, 50°C
pH 10.0, 50°C, calculated by BRENDA

substrate: azocasein


organism: Shouchella clausii


organism: Solanum lycopersicum

substrate_name: Unknown

substrate: Unknown


organism: synthetic construct

assay_conditions_summary: pH 7.8; 298.15 K; buffer 10 mM potassium phosphate

proof_lines: 3.5 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C

proof_lines: subtilisin without bound lactose, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C

proof_lines: 2.2 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C


enzyme_display_name: penicillin amidase

substrate_name: Penicillin V

enzyme: penicillin amidase

substrate: Penicillin V


organism: Escherichia coli

substrate_name: (S)-N-acetylphenylglycine

substrate: (S)-N-acetylphenylglycine


organism: Fusarium oxysporum


organism: Fusarium sp.


organism: Achromobacter sp.

substrate_name: penicillin G

substrate: penicillin G


organism: Achromobacter xylosoxidans


organism: Actinoplanes utahensis


organism: Alcaligenes faecalis

substrate_name: 6-nitro-3-phenylacetamidobenzoic acid

proof_lines:
pH 7.5, 25°C, enzyme form PA5..3
pH 7.5, 25°C, enzyme form PA5..5
pH 7.0, 25°C

substrate: 6-nitro-3-phenylacetamidobenzoic acid


organism: Kluyvera cryocrescens

substrate_name: benzylpenicillin

proof_lines: pH 7.5, 40°C, presence of 10 mM phenylglyoxal

proof_lines: pH 7.5, 40°C, presence of 50 mM 2,3-butanedione

proof_lines: unmodified enzyme, enzyme modified with N-bromosuccinamide or enzyme modified with 2-hydroxy 5-nitrobenzylbromide

substrate: benzylpenicillin


organism: Lysinibacillus sphaericus


organism: Mucor griseocyanus


organism: Priestia megaterium


organism: Providencia rettgeri


organism: Pseudobacillus badius

substrate_name: ampicillin

substrate: ampicillin


organism: Pseudomonas melanogenum

substrate_name: cephadrine

substrate: cephadrine


organism: Streptomyces sp.


enzyme_display_name: N-acyl-D-amino-acid deacylase

organism: Streptomyces olivaceus

substrate_name: N-Acetyl-D-Trp

enzyme: N-acyl-D-amino-acid deacylase

substrate: N-Acetyl-D-Trp


organism: Trichoderma sp.

substrate_name: N-acetyl-D-methionine

substrate: N-acetyl-D-methionine


organism: Achromobacter denitrificans

substrate_name: N-Chloroacetyl-D-Val

substrate: N-Chloroacetyl-D-Val


substrate_name: N-Acetyl-D-Leu

substrate: N-Acetyl-D-Leu


substrate_name: N-Acetyl-D-Met

proof_lines:
soluble enzyme
immobilized enzyme

substrate: N-Acetyl-D-Met


organism: Rhodococcus armeniensis

substrate_name: N-acetyl-D-leucine

substrate: N-acetyl-D-leucine


enzyme_display_name: arylacetonitrilase

enzyme: arylacetonitrilase


organism: Alcaligenes sp. MTCC 10675

substrate_name: mandelonitrile

substrate: mandelonitrile


organism: Arabidopsis thaliana


organism: Aspergillus niger

substrate_name: phenyl acetonitrile

substrate: phenyl acetonitrile


organism: Rhizorhabdus wittichii

substrate_name: phenylglycinonitrile

substrate: phenylglycinonitrile


organism: Trichophyton benhamiae


organism: Bradyrhizobium japonicum

substrate_name: phenylacetonitrile

substrate: phenylacetonitrile


organism: Brassica napus

substrate_name: phenylpropionitrile

substrate: phenylpropionitrile


organism: Burkholderia cenocepacia


organism: Fusarium haematococcum


organism: Luminiphilus syltensis


organism: Neurospora crassa


organism: Pseudomonas fluorescens


organism: Pseudomonas putida


enzyme_display_name: inorganic diphosphatase

organism: Aspergillus oryzae

substrate_name: diphosphate

proof_lines:
pH 6.9
pH 3

enzyme: inorganic diphosphatase

substrate: diphosphate


organism: Blastochloris viridis


organism: Bos taurus

substrate_name: Imidodiphosphate

substrate: Imidodiphosphate


organism: Capsicum annuum


organism: Cricetinae

proof_lines: diphosphate at 1 mM, enzyme from astroblasts

proof_lines: diphosphate at 1 mM, enzyme from neuroblasts


organism: Desulfovibrio desulfuricans

assay_conditions_summary: pH 6


organism: Dunaliella salina


organism: Entamoeba histolytica


organism: Escherichia coli

proof_lines: pH 7.5, 25°C, hexameric mutant E145Q, 5 mM Mg2+


organism: Gossypium hirsutum

substrate_name: Unknown

proof_lines: pH 7.2, 37°C, fluoride inhibition kinetics

substrate: Unknown


organism: Helicobacter pylori

substrate_name: Mg-diphosphate

substrate: Mg-diphosphate


organism: Homo sapiens


organism: Hypophthalmichthys nobilis


organism: Methanothermobacter thermautotrophicus


organism: Streptococcus salivarius


organism: Sulfolobus acidocaldarius


organism: Sus scrofa


organism: Thermococcus onnurineus


organism: Mus sp.


organism: Neomoorella thermoacetica


organism: Saccharomyces cerevisiae

substrate_name: ATP

assay_conditions_summary: pH 7.2; 298.15 K; cofactor ATP

proof_lines: less than 0.005/sec, pH 7.2, 25°C, in the presence of Mg2+

substrate: ATP


organism: Saccharum officinarum

proof_lines:
isoform 1
isoform 2


enzyme_display_name: orotidine-5'-phosphate decarboxylase

organism: Crithidia luciliae

substrate_name: orotidine 5'-phosphate

proof_lines:
membrane-bound enzyme
solubilized enzyme

enzyme: orotidine-5'-phosphate decarboxylase

substrate: orotidine 5'-phosphate


assay_conditions_summary: pH 7

proof_lines: pH 7, constant above pH 7, declines below pH 7


proof_lines: in 50 mM Tris (pH 7.5), 20 mM dithiothreitol, and 40 mM NaCl, at 22°C


proof_lines: orotidine-5-phosphate decarboxylase domain of the bifunctional enzyme UMP synthase


substrate_name: 4-Thioorotidine 5'-phosphate

proof_lines: 22-25°C, pH 7.2, recombinant ODCase expressed in Escherichia coli

proof_lines: pH 7.2, 25°C, not affected by NaCl concentration

substrate: 4-Thioorotidine 5'-phosphate


substrate_name: Orotidine

proof_lines: value is higher than 30 mM, pH 7.2, 23°C, mutant enzyme C155S

substrate: Orotidine


substrate_name: 5-Fluoroorotidine 5'-phosphate

proof_lines: mutant R235A, pH 7.1, 25°C, presence of 0.1 M NaCl

proof_lines: mutant R235A, pH 7.1, 25°C, presence of 0.1 M NCl

substrate: 5-Fluoroorotidine 5'-phosphate


organism: Staphylococcus aureus


proof_lines: in 50 mM Tris, pH 7.5, 20 mM dithiothreitol, and 40 mM NaCl, at 55°C


organism: Mus musculus

proof_lines: orotidine-5-phosphate decarboxylase domain of the bifunctional enzyme UMP synthase, 20°C

proof_lines: orotidine-5-phosphate decarboxylase domain of the bifunctional enzyme UMP synthase, 37°C


organism: Plasmodium falciparum


organism: Pseudomonas aeruginosa


enzyme_display_name: phosphoenolpyruvate carboxylase

organism: Cicer arietinum

enzyme: phosphoenolpyruvate carboxylase


organism: Coccochloris peniocystis


organism: Coleataenia prionitis


organism: Annona cherimola

substrate_name: phosphoenolpyruvate

proof_lines: pH 7.8, enzyme from mesocarp, stored in air

proof_lines: pH 7.8, enzyme from mesocarp, stored in 20% CO2

substrate: phosphoenolpyruvate


organism: Bienertia sinuspersici

proof_lines: leaves are collected after 5 h in the dark, pH: 8.0

proof_lines: leaves are collected after 5 h in the light, pH: 8.0


organism: Brassica napus

proof_lines: pH 8.4, 24°C, pH 7.3, 24°C, enzyme from cells grown in presence of phosphate or in absence of phosphate, assay in presence of glycerol

proof_lines: pH 7.3, 24°C, enzyme from cells grown in absence of phosphate, assay in absence of glycerol

proof_lines: pH 7.3, 24°C, enzyme from cells grown in presence of phosphate, assay in absence of glycerol


organism: Brassica rapa subsp. oleifera


organism: Acetobacter aceti


organism: Amaranthus hypochondriacus

proof_lines: pH 7.3, addition of 30 mM phosphate, enzyme from dark-adapted leaves

proof_lines: pH 7.3, no addition of phosphate, enzyme from dark-adapted leaves


organism: Amaranthus viridis


organism: Ancylobacter novellus


organism: Cucumis sativus

proof_lines: pH 8.0, 25°C, enzyme from roots grown in absence of Fe2+

proof_lines: pH 8.0, 25°C, enzyme from roots grown in presence of Fe2+


organism: Escherichia coli


organism: Fagus sylvatica

proof_lines: stem leaf


organism: Haloxylon persicum

proof_lines: leaves are collected after 5 h into the dark, pH: 8.0

proof_lines: leaves are collected after 5 h into the light, pH: 8.0


organism: Hydrilla verticillata

proof_lines: C3 extract, dark-harvested PEPC in the absence of D-glucose 6-phosphate

proof_lines: C3 extract, light-harvested PEPC in the absence of D-glucose 6-phosphate

proof_lines: C3 extract, light-harvested PEPC in the presence of 5 mM D-glucose 6-phosphate


organism: Kalanchoe fedtschenkoi

proof_lines:
at 40°C
in absence of effectors, at 25°C
at 14°C


organism: Lupinus luteus

proof_lines:
enzyme form PEPC II
enzyme form PEPC III
enzyme form PEPC I


organism: Malus sp.

proof_lines: golden delicious, vascular bundle or seed


organism: Methanothermobacter thermautotrophicus


organism: Methanothermus sociabilis


organism: Molinema dessetae


organism: Oceanimonas smirnovii

proof_lines: 23°C, pH not specified in the publication


organism: Oryza sativa

proof_lines:
isoform Osppc2a, at pH 7.3 and 30°C
isoform Osppc2a, at pH 8.0 and 30°C
isoform Osppc4, at pH 8.0 and 30°C


organism: Paspalum vaginatum

proof_lines:
wild-type, pH 7.6, 25°C
wild-type, Hill value 1.1, pH 7.6, 25°C


organism: Phaeodactylum tricornutum


organism: Picosynechococcus sp. PCC 7002


organism: Plasmodium berghei


organism: Rhodopseudomonas sp.

assay_conditions_summary: cofactor CoA


organism: Ricinus communis

proof_lines:
pH 8, PEPC1
pH 8, PEPC2
pH 7.3, PEPC1


organism: Sorghum bicolor

proof_lines:
10 days post-imbibition, pH 7.3, 30°C
extract from germinated seed
35 days post-anthesis, pH 7.3, 30°C


organism: Spinacia oleracea

proof_lines:
with Mg2+ as activator
with Mn2+ as activator
without activator


organism: Steinchisma hians


organism: Suaeda aralocaspica


organism: Suaeda linifolia


organism: Thermosynechococcus vestitus


organism: Thiobacillus thioparus


organism: Vicia faba

proof_lines:
pH 7
pH 8.3


organism: Zea mays subsp. mays


enzyme_display_name: phosphoenolpyruvate carboxykinase (GTP)

organism: Aquarana catesbeiana

substrate_name: ITP

proof_lines:
exchange reaction
pH 6.3
CO2 in form of HCO3-

enzyme: phosphoenolpyruvate carboxykinase (GTP)

substrate: ITP


organism: Ascaris suum

substrate_name: GDP

proof_lines: with GDP as cosubstrate, Mn2+ does not affect the Km

proof_lines: with IDP as cosubstrate, Mn2+ does not affect the Km

substrate: GDP


organism: Corynebacterium glutamicum

proof_lines:
pH 6.8
CO2 in form of HCO3-


organism: Euglena gracilis

proof_lines: enzyme from heterotrophically grown cells


organism: Gallus gallus

proof_lines: enzyme form M1 from embryo and enzyme form S2 from young chicken


organism: Homo sapiens

proof_lines: in 100 mM HEPES-NaOH, pH 8, 3.6 mM L-malate, 10 mM dithiothreitol, 2 mM MgCl2, 0.2 mM MnCl2, at 37°C

proof_lines: in 100 mM HEPES-NaOH, pH 7.2, 100 mM KHCO3, 10 mM dithiothreitol, 2 mM MgCl2, 0.2 mM MnCl2, at 37°C


organism: Modiolus demissus

substrate_name: GTP

proof_lines: in presence of 1 mM MnCl2 and 1 mM MgCl2

substrate: GTP


organism: Perumytilus purpuratus

substrate_name: IDP

proof_lines:
pH 7, in presence of 2.3 mM Mn2+
CO2 in form of HCO3-

substrate: IDP


organism: Raillietina echinobothrida

proof_lines: with GDP as cosubstrate, in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication

proof_lines: with IDP as cosubstrate, in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication


organism: Rattus norvegicus


organism: Squalus acanthias

proof_lines:
in presence of 0.0015 mM Mn2+
in presence of 0.0013 mM Mn2+
in presence of 0.002 mM Mn2+


enzyme_display_name: 2-dehydro-3-deoxy-L-fuconate aldolase

organism: Campylobacter jejuni subsp. jejuni

enzyme: 2-dehydro-3-deoxy-L-fuconate aldolase


enzyme_display_name: (S)-hydroxynitrile lyase

organism: Hevea brasiliensis

substrate_name: (S)-mandelonitrile

assay_conditions_summary: pH 5; 298.15 K; buffer pH 6

proof_lines: in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication

proof_lines: in 50 mM phosphate buffer pH 6.0, at 25°C

proof_lines: in 50 mM phosphate buffer pH 6.0, at 22°C

enzyme: (S)-hydroxynitrile lyase

substrate: (S)-mandelonitrile


organism: Manihot esculenta

substrate_name: mandelonitrile

substrate: mandelonitrile


assay_conditions_summary: pH 5; 313.15 K; buffer 50 mM citrate


enzyme_display_name: tryptophan synthase

organism: Thermotoga maritima

substrate_name: L-serine

proof_lines: pH 7.5, 80°C, recombinant (TrpA-TrpB1)2 tetrameric complex, beta-reaction

proof_lines: pH 7.5, 80°C, recombinant TrpB1, beta-reaction

enzyme: tryptophan synthase

substrate: L-serine


organism: Saccharomyces cerevisiae

substrate_name: indole

substrate: indole


organism: Salmonella enterica subsp. enterica serovar Typhimurium

proof_lines: beta-reaction and overall-reaction, mutant D305A enzyme, pH 7.8, 25°C

proof_lines: beta-reaction, mutant D305A, pH 7.8, 25°C

proof_lines: overall-reaction, mutant D305A enzyme, pH 7.8, 25°C, in presence of K+


substrate_name: 1-(indol-3-yl)glycerol 3-phosphate

proof_lines: overall-reaction, mutant R141A, pH 7.8, 25°C

proof_lines: beta-reaction, mutant R141A, pH 7.8, 25°C

proof_lines: alpha-reaction, mutant R141A, pH 7.8, 25°C

substrate: 1-(indol-3-yl)glycerol 3-phosphate


proof_lines: 37°C, beta-elimination, enzyme in solution

proof_lines: in the presence of 100 mM CsCl, at pH 6.5 and 25°C


enzyme_display_name: alanine racemase

substrate_name: D-alanine

enzyme: alanine racemase

substrate: D-alanine


organism: Enterococcus faecalis

substrate_name: D-Ala

proof_lines: pH and temperature not specified in the publication

substrate: D-Ala


organism: Escherichia coli

proof_lines: in 50 mM potassium phosphate buffer pH 7.4, at 30°C


organism: Geobacillus stearothermophilus

substrate_name: L-Ala

substrate: L-Ala


organism: Procambarus clarkii


organism: Pseudomonas aeruginosa


organism: Pseudomonas fluorescens


organism: Pseudomonas putida

substrate_name: L-alanine

assay_conditions_summary: pH 9; 310.15 K; buffer pH 7

substrate: L-alanine


organism: Medicago sativa

proof_lines:
Vmax = 0.44 mol/s/kg
Vmax = 1.02 mol/s/kg


organism: Mycobacterium avium


organism: Mycobacterium tuberculosis

substrate_name: Unknown

proof_lines: in 100 mM Tris-Tricine, pH 8.5, temperature not specified in the publication

substrate: Unknown


organism: Penaeus monodon


enzyme_display_name: aralkylamine N-acetyltransferase

substrate_name: histamine

assay_conditions_summary: pH 7; 298.15 K; cofactor CoA

enzyme: aralkylamine N-acetyltransferase

substrate: histamine


substrate_name: acetyl-CoA

substrate: acetyl-CoA


substrate_name: octopamine

substrate: octopamine


substrate_name: propionyl-CoA

substrate: propionyl-CoA


organism: Mycolicibacterium smegmatis


organism: Oryza sativa

substrate_name: Acetyl-CoA; Serotonin

substrate: Acetyl-CoA; Serotonin


source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

assay_conditions_summary: pH 8.8; 318.15 K; cofactor CoA


organism: Ovis aries

substrate_name: Coenzyme A

assay_conditions_summary: pH 6.8; 303.15 K; cofactor CoA

substrate: Coenzyme A


substrate_name: N-Bromoacetyltryptamine

substrate: N-Bromoacetyltryptamine


enzyme_display_name: carnitine O-palmitoyltransferase

organism: Homo sapiens

substrate_name: Palmitoyl-CoA; L-Carnitine

enzyme: carnitine O-palmitoyltransferase

substrate: Palmitoyl-CoA; L-Carnitine


proof_lines: chimaera in which the distinctive N-terminal segment of ovine m-BPT 1 is replaced with that from rat M-CPT 1


organism: Rattus norvegicus


organism: Sus scrofa


substrate_name: Acetyl-CoA

substrate: Acetyl-CoA


substrate_name: Tryptamine

substrate: Tryptamine


substrate_name: alpha-Methyltryptamine

substrate: alpha-Methyltryptamine


substrate_name: serotonin

substrate: serotonin


substrate_name: Acetyl-CoA; Tryptamine

substrate: Acetyl-CoA; Tryptamine


enzyme_display_name: corrinoid adenosyltransferase

substrate_name: ATP

assay_conditions_summary: cofactor ATP

enzyme: corrinoid adenosyltransferase

substrate: ATP


organism: Lactobacillus reuteri

substrate_name: Cob(I)alamin

substrate: Cob(I)alamin


assay_conditions_summary: pH 8; 310.15 K; cofactor ATP


substrate_name: CTP

substrate: CTP


substrate_name: dATP

substrate: dATP


substrate_name: UTP

substrate: UTP


substrate_name: ITP

substrate: ITP


source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

source_databases_merged: sabio_rk brenda skid


substrate_name: ATP; Cob(I)alamin

substrate: ATP; Cob(I)alamin


substrate_name: GTP

substrate: GTP


substrate_name: Cob(II)alamin

substrate: Cob(II)alamin


substrate_name: Cob(II)alamin; ATP

substrate: Cob(II)alamin; ATP


enzyme_display_name: kynurenine--oxoglutarate transaminase

smiles: CC(C)CC(=O)C(=O)O

enzyme: kynurenine--oxoglutarate transaminase


substrate_name: oxaloacetate

smiles: O=C(O)CC(=O)C(=O)O

substrate: oxaloacetate


substrate_name: Aminobutyrate

substrate: Aminobutyrate


substrate_name: L-Glutamate

substrate: L-Glutamate


substrate_name: L-Tryptophan

substrate: L-Tryptophan


substrate_name: L-Phenylalanine

substrate: L-Phenylalanine


substrate_name: L-Tyrosine

substrate: L-Tyrosine


substrate_name: Glyoxylate; L-Kynurenine

substrate: Glyoxylate; L-Kynurenine


substrate_name: L-kynurenine

substrate: L-kynurenine


organism: Mus musculus

substrate_name: L-tryptophan

substrate: L-tryptophan


smiles: O=C(O)CCC(=O)C(=O)O


substrate_name: ATP

substrate: ATP


substrate_name: Cob(I)alamin

substrate: Cob(I)alamin


organism: Salmonella enterica

substrate_name: cob(II)inamide

substrate: cob(II)inamide


organism: Thermoplasma acidophilum

substrate_name: dATP

assay_conditions_summary: pH 8; 343.15 K; cofactor ATP

proof_lines: pH 8.0, 70°C, wild-type enzyme, in presence of Mg2+

substrate: dATP


substrate_name: Cobalamin

proof_lines:
Raw SKiD Env: Temp=70°C, pH=8
Raw SKiD Env: Temp=-----, pH=-----

substrate: Cobalamin


organism: Aedes aegypti

substrate_name: 2-Oxoglutarate; L-Kynurenine

substrate: 2-Oxoglutarate; L-Kynurenine


substrate_name: Asparagine

substrate: Asparagine


substrate_name: Serine

substrate: Serine


substrate_name: Alanine

substrate: Alanine


substrate_name: methionine

substrate: methionine


substrate_name: Aminobutyrate

substrate: Aminobutyrate


smiles: CCC(C)C(=O)C(=O)O


smiles: CCCCC(=O)C(=O)[O-]


smiles: CCCCC(=O)C(=O)O


smiles: O=C(O)C(=O)CS


substrate_name: Glyoxylate

smiles: O=CC(=O)O

substrate: Glyoxylate


substrate_name: L-Leucine

substrate: L-Leucine


substrate_name: L-Alanine

substrate: L-Alanine


substrate_name: hydroxyphenylpyruvate

substrate: hydroxyphenylpyruvate


substrate_name: glyoxylate

substrate: glyoxylate


substrate_name: Mercaptopyruvate

substrate: Mercaptopyruvate


substrate_name: 2-oxocaproic acid

substrate: 2-oxocaproic acid


substrate_name: phenylpyruvate

substrate: phenylpyruvate


substrate_name: pyruvate

smiles: CC(=O)C(=O)O

substrate: pyruvate


substrate_name: alpha-oxo-gamma-methiol-butyric acid

smiles: CSCCC(=O)C(=O)O

substrate: alpha-oxo-gamma-methiol-butyric acid


substrate_name: 2-oxoisocaproic acid

substrate: 2-oxoisocaproic acid


substrate_name: L-kynurenine

substrate: L-kynurenine


substrate_name: L-Methionine

substrate: L-Methionine


substrate_name: L-Phenylalanine

substrate: L-Phenylalanine


substrate_name: oxaloacetate

substrate: oxaloacetate


substrate_name: L-serine

substrate: L-serine


substrate_name: L-alanine

substrate: L-alanine


substrate_name: L-tyrosine

substrate: L-tyrosine


smiles: CCC(=O)C(=O)O


substrate_name: L-Cysteine

substrate: L-Cysteine


substrate_name: L-glutamine

substrate: L-glutamine


substrate_name: L-Asparagine

substrate: L-Asparagine


substrate_name: L-histidine

substrate: L-histidine


substrate_name: glyoxylate

substrate: glyoxylate


substrate_name: Mercaptopyruvate

substrate: Mercaptopyruvate


substrate_name: phenylpyruvate

substrate: phenylpyruvate


substrate_name: 2-oxocaproic acid

substrate: 2-oxocaproic acid


substrate_name: pyruvate

substrate: pyruvate


substrate_name: hydroxyphenylpyruvate

substrate: hydroxyphenylpyruvate


substrate_name: Pyruvate; L-Kynurenine

substrate: Pyruvate; L-Kynurenine


substrate_name: 2-Oxoglutarate; L-Kynurenine

substrate: 2-Oxoglutarate; L-Kynurenine


organism: Arabidopsis thaliana

substrate_name: UTP; D-Glucose 1-phosphate

proof_lines:
pyrophosphorolysis
UDP-glucose synthesis
Raw SKiD Env: Temp=-----, pH=-----

substrate: UTP; D-Glucose 1-phosphate


substrate_name: UTP; alpha-D-Glucose 1-phosphate

substrate: UTP; alpha-D-Glucose 1-phosphate


organism: Bos taurus


organism: Caenorhabditis elegans

substrate_name: alpha-D-glucose 1-phosphate

substrate: alpha-D-glucose 1-phosphate


substrate_name: dTTP

substrate: dTTP


substrate_name: UTP

substrate: UTP


organism: Cucumis melo

substrate_name: Diphosphate; UDPglucose

substrate: Diphosphate; UDPglucose


organism: Escherichia coli


substrate_name: D-Glucose 1-phosphate; UTP

substrate: D-Glucose 1-phosphate; UTP


organism: Synechocystis sp.


organism: Thermocrispum agreste


proof_lines:
pH 7.5, 37°C, recombinant enzyme
Raw SKiD Env: Temp=37°C, pH=7.5
Raw SKiD Env: Temp=37°C, pH=7.8


organism: Hordeum vulgare


organism: Nostoc sp.

substrate_name: diphosphate

substrate: diphosphate


organism: Solanum tuberosum


organism: Sulfurisphaera tokodaii


organism: Drosophila melanogaster

substrate_name: tryptamine (for the enzyme without substrate bound to the regulatory site and with acetyl CoA as cosubstrate)

assay_conditions_summary: pH 8; cofactor CoA

proof_lines: kcat is 1.3 sec(-1) for acetyl-CoA (at pH 8 and with serotonin as cosubstrate) (PubMed:24444601). kcat is 0.33 sec(-1) for butanoyl-CoA (at pH 8 and with serotonin as cosubstrate) (PubMed:24444601). kcat is 0.16 sec(-1) for palmitoyl-CoA (a


substrate_name: tryptamine (in transfected COS-7 cell extracts)

assay_conditions_summary: pH 7.2; cofactor CoA

proof_lines: kcat is 21.9 sec(-1) with tryptamine and acetyl-CoA as substrate (at pH 7.2).

proof_lines: kcat is 19 sec(-1) for tyramine (at pH 8 and with acetyl-CoA as cosubstrate) (PubMed:25406072). kcat is 13 sec(-1) for octapamine (at pH 8 and with acetyl-CoA as cosubstrate) (PubMed:25406072). kcat is 17.6 sec(-1) for dopamine (at pH 8 and


proof_lines: kcat is 206 min(-1) for cysteine (at 45 degrees Celsius, at pH 8.5 and with ketobutyrate as cosubstrate) (PubMed:15556614). kcat is 139 min(-1) for tyrosine (at 45 degrees Celsius, at pH 8.5 and with ketobutyrate as cosubstrate) (PubMed:155


organism: Bacillus subtilis (strain 168)

proof_lines: kcat is 1.06 sec(-1) with glucose-1-phosphate as substrate. kcat is 1.04 sec(-1) with UTP as substrate.


proof_lines: kcat is 0.264 sec(-1) with glucose-1-phosphate as substrate. kcat is 0.293 sec(-1) with UTP as substrate.


organism: Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)

proof_lines: kcat is 69.7 sec(-1) for GalN-1-P acetyltransferase activity. kcat is 123.2 sec(-1) for GlcN-1-P acetyltransferase activity.


organism: Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

substrate_name: UTP (at pH 7.5 and 37 degrees Celsius)

proof_lines: kcat is 28 sec(-1) (at pH 7.5 and 37 degrees Celsius).

substrate: UTP (at pH 7.5 and 37 degrees Celsius)


organism: Sus scrofa

substrate_name: L-carnitine

proof_lines: E17D mutant protein, mitochondrial preparation

substrate: L-carnitine


organism: Pyrococcus horikoshii

proof_lines:
0.1 mM L-kynurenine, pH 7.5, 25°C
pH 7.5, 25°C


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: D-Ala

proof_lines:
alr encoded enzyme
dadB encoded enzyme
alr gene encoded

substrate: D-Ala


organism: Staphylococcus aureus

substrate_name: L-alanine

proof_lines: pH and temperature not specified in the publication

substrate: L-alanine


organism: Streptomyces lavendulae

substrate_name: D-serine

substrate: D-serine


organism: Thalassiosira sp.

substrate_name: D-alanine

substrate: D-alanine


enzyme_display_name: chitinase

organism: Serratia marcescens

enzyme: chitinase


substrate_name: H2O; Chitobiose

substrate: H2O; Chitobiose


organism: Trichoderma harzianum

substrate_name: H2O; Chitin

substrate: H2O; Chitin


source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0


organism: Zea mays

substrate_name: H2O; Tetra-N-acetylchitotetraose

substrate: H2O; Tetra-N-acetylchitotetraose


organism: Saccharolobus solfataricus

substrate_name: amylopectin

proof_lines: pH 6.5, 75°C, calculated as microMol maltose released from the substrate, in absence of DMSO

proof_lines: pH 5.5, 75°C, calculated as microMol maltose released from the substrate, in presence of DMSO

proof_lines: pH 5.5, 75°C, calculated as microMol maltose released from the substrate, in absence of DMSO

substrate: amylopectin


organism: Aspergillus fumigatus


organism: Bacillus licheniformis


organism: Bacillus thuringiensis


organism: Gallus gallus


organism: Homo sapiens

proof_lines:
mutant N45D/D47N/R61M, pH 7.0, 37°C
mutant N45D/D47N/R61M, pH 4.6, 37°C
mutant N45D/D47N/R61M, pH 2.2, 37°C


organism: Listeria innocua


organism: Macaca fascicularis

substrate_name: 4-methylumbelliferyl chitobioside

substrate: 4-methylumbelliferyl chitobioside


organism: Ostrinia furnacalis


proof_lines:
domain ChtII-C1, at pH 6.0 and 30°C
domain ChtII-C2, at pH 6.0 and 30°C
domain ChtII-C1C2, at pH 6.0 and 30°C


organism: Saccharomyces cerevisiae


organism: Schistocerca gregaria


organism: Arabidopsis thaliana

substrate_name: Phosphoenolpyruvate; H2O; CO2

substrate: Phosphoenolpyruvate; H2O; CO2


organism: Brassica napus


organism: Corynebacterium glutamicum

substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


organism: Escherichia coli


organism: Flaveria pringlei


organism: Flaveria trinervia


organism: Methanothermobacter thermautotrophicus


organism: Sorghum bicolor


substrate_name: Phosphate; Oxaloacetate

substrate: Phosphate; Oxaloacetate


organism: Zea mays


substrate_name: CO2; Phosphoenolpyruvate; H2O

substrate: CO2; Phosphoenolpyruvate; H2O


enzyme_display_name: chloramphenicol O-acetyltransferase

organism: Unknown

substrate_name: chloramphenicol

enzyme: chloramphenicol O-acetyltransferase

substrate: chloramphenicol


organism: Bombyx mori

substrate_name: Glycolchitin; H2O

proof_lines:
isoenzyme 1
isoenzyme 2

substrate: Glycolchitin; H2O


substrate_name: glycogen phosphorylase limit dextrin

substrate: glycogen phosphorylase limit dextrin


proof_lines: dephosphorylated isozyme PPC1, at pH 7.3


proof_lines:
pH 8.0
isoenzyme PC-I
isoenzyme PC-II


organism: Homo sapiens

substrate_name: aminoadipate

substrate: aminoadipate


organism: Mus musculus

substrate_name: glutamine

substrate: glutamine


organism: Rattus norvegicus

substrate_name: L-kynurenine (with 2-oxoglutarate as cosubstrate)


substrate_name: L-kynurenine

substrate: L-kynurenine


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: tryptophan

substrate: tryptophan


substrate_name: glucose 1-phosphate

substrate: glucose 1-phosphate


substrate_name: MgUTP

substrate: MgUTP


organism: Aspergillus fumigatus


organism: Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)

substrate_name: SR-linked chitopentaose

substrate: SR-linked chitopentaose


organism: Glutamicibacter uratoxydans

substrate_name: Colloidal Chitin

sequence: ADGTITFNGKVTDK

wild_type_sequence: ADGTITFNGKVTDK

substrate: Colloidal Chitin


substrate_name: carnitine

substrate: carnitine


substrate_name: hexadecanoyl-CoA

substrate: hexadecanoyl-CoA


organism: Arabidopsis thaliana

substrate_name: serotonin

substrate: serotonin


substrate_name: tryptamine

substrate: tryptamine


organism: Oryza sativa subsp. japonica

substrate_name: serotonin (at 55 degrees Celsius)

substrate: serotonin (at 55 degrees Celsius)


organism: Ovis aries

substrate_name: acetyl-CoA

substrate: acetyl-CoA


substrate_name: Unknown

substrate: Unknown


organism: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)

substrate_name: phosphoenolpyruvate

verification_status: corrected

substrate: phosphoenolpyruvate

status: corrected


organism: Paenibacillus xylanexedens

substrate_name: colloidal chitin

proof_lines: kcat is 0.08 sec(-1) with colloidal chitin as substrate.

substrate: colloidal chitin


organism: Punica granatum

proof_lines: kcat/KM is 0.119 M(-1)sec(-1) for native protein, 0.130 M(-1)sec(-1) for native protein with 10 mM Ca(2+) and 0.116 M(-1)sec(-1) for EDTA-treated protein, using 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside (4-MU(GlcNAc)(3)) a


organism: Yersinia entomophaga

proof_lines: In the intact toxin complex the combined chitinase activity of Chi1 and Chi2 is slightly reduced.


organism: Zea mays

substrate_name: tetra-N-acetylchitotetraose (at 37 degrees Celsius and pH 6)

proof_lines: kcat is 56.27 sec(-1) with tetra-N-acetylchitotetraose as substrate (at 37 degrees Celsius and pH 6). kcat is 55.67 sec(-1) with tetra-N-acetylchitotetraose as substrate (at 37 degrees Celsius and pH 4). kcat is 69.01 sec(-1) with tetra-N-a


organism: Pseudomonas aeruginosa


substrate_name: chloramphenicol

substrate: chloramphenicol


organism: Medicago truncatula


organism: Metarhizium anisopliae

substrate_name: p-nitrophenyl beta-N-diacetylchitobiose

substrate: p-nitrophenyl beta-N-diacetylchitobiose


organism: Streptomyces violaceusniger

sequence: GDGTGPGPGP

wild_type_sequence: GDGTGPGPGP


organism: Trichoderma harzianum


substrate_name: PEP (Phospho-PPC1 at pH 7.3 and 25 degrees Celsius)


organism: Flaveria trinervia


organism: Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)


organism: Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)


organism: Proteus mirabilis


proof_lines:
enzyme variant CATA1
enzyme variant CATB1
enzyme variant CATB5


organism: Staphylococcus aureus


proof_lines:
enzyme variant D
enzyme variant C


organism: Saccharomyces cerevisiae

substrate_name: 6-O-alpha-D-glucosyl cyclomaltoheptaose

substrate: 6-O-alpha-D-glucosyl cyclomaltoheptaose


organism: Agrobacterium tumefaciens


organism: Bacillus pumilus


organism: Clostridium perfringens


organism: Escherichia coli


substrate_name: isobutanol

substrate: isobutanol


proof_lines: pH 7.0, 60°C, recombinant wild-type enzyme


proof_lines:
enzyme variant CAT III
enzyme variant CAT I
enzyme variant CATIII


organism: Flavobacterium sp.


organism: Haemophilus parainfluenzae


organism: Morganella morganii


substrate_name: amylopectin beta-dextrin

proof_lines:
phi-dextrin from shellfish glycogen
beta-dextrin from shellfish glycogen

substrate: amylopectin beta-dextrin


proof_lines:
enzyme from plasmid pSCS6
enzyme from plasmid pSCS7


organism: Staphylococcus epidermidis


organism: Staphylococcus sp.


organism: Streptococcus agalactiae


organism: Nostoc punctiforme

substrate_name: maltodecaose

assay_conditions_summary: pH 7.5; 303.15 K; buffer 50 mM sodium phosphate

proof_lines: in 50 mM sodium phosphate buffer (pH 7.5) at 30°C

substrate: maltodecaose


organism: Oryctolagus cuniculus

substrate_name: glycogen phosphorylase limit dextrin

proof_lines:
phi-dextrin
phi-dextrin from shellfish glycogen
branched pentasaccharide B5, pH 6.6

substrate: glycogen phosphorylase limit dextrin


organism: Saccharolobus shibatae

assay_conditions_summary: pH 5.5; 343.15 K; buffer 50 mM sodium acetate

proof_lines: at 70°C in 50 mM sodium acetate buffer (pH 5.5)


organism: Saccharolobus solfataricus

substrate_name: maize amylopectin

proof_lines: pH 5.0, 70°C, recombinant wild-type enzyme

substrate: maize amylopectin


organism: Streptococcus pneumoniae


organism: Streptomyces acrimycini


organism: Vibrio anguillarum


organism: Tolypocladium inflatum

substrate_name: L-alanine

substrate: L-alanine


organism: Salmonella enterica

substrate_name: ATP; Cob(I)alamin

assay_conditions_summary: pH 7; 310.15 K; cofactor ATP

proof_lines:
pH 7.0, 37°C, recombinant enzyme
at pH 7.0 and 37°C

substrate: ATP; Cob(I)alamin


organism: Suaeda eltonica

assay_conditions_summary: pH 8

proof_lines: leaves are collected after 5 h into the light, pH: 8.0

proof_lines: leaves are collected after 5 h into the dark, pH: 8.0


organism: Umbilicus rupestris

proof_lines: pH 8.2, enzyme from water stressed plants

proof_lines: pH 7.2, enzyme from water stressed plants


organism: Xylosalsola richteri


organism: Bienertia sinuspersici

proof_lines: leaves are collected after 5 h in the dark, pH: 8.0

proof_lines: leaves are collected after 5 h in the light, pH: 8.0


organism: Corynebacterium glutamicum

proof_lines: protein with N-terminal His-tag, pH 7.5, 30Â°C

proof_lines: protein with C-terminal His-tag, pH 7.5, 30Â°C


organism: Ricinus communis

proof_lines:
mutant enzyme S425A, at pH 8.0 and 25°C
mutant enzyme S425A, at pH 7.3 and 25°C


organism: Suaeda aralocaspica


organism: Trichinella spiralis

substrate_name: diphosphate

assay_conditions_summary: pH 7.5; 328.15 K; cofactor ATP

substrate: diphosphate


organism: Trypanosoma cruzi

substrate_name: Triphosphate

proof_lines:
with 3 mM Co2+, at pH 6.5 and 30°C
with 3 mM Zn2+, at pH 6.5 and 30°C
with 3 mM Mg2+, at pH 8.5 and 30°C

substrate: Triphosphate


organism: Vibrio cholerae serotype O1

proof_lines: in the presence of 10 mM Mg2+, in 50 mM Mes-NaOH, at pH 6.5 and 25°C

proof_lines: in the presence of 0.005 mM Mn2+, in 50 mM HEPES-NaOH, at pH 7.5 and 25°C

proof_lines: in the presence of 5 mM Mg2+, in 50 mM Tris-HCl, at pH 9.0 and 25°C


enzyme_display_name: myeloperoxidase

organism: Homo sapiens

enzyme: myeloperoxidase


organism: Rattus norvegicus

substrate_name: H2O; Diphosphate

substrate: H2O; Diphosphate


organism: Rhodopseudomonas palustris

substrate_name: Diphosphate; H2O

substrate: Diphosphate; H2O


organism: Sorghum bicolor


organism: Bos taurus

substrate_name: SCN-

substrate: SCN-


substrate_name: benzyl methyl sulfide

assay_conditions_summary: pH 5; 293.15 K; buffer 50 mM acetate

proof_lines: in the absence of NaCl, in 50 mM acetate buffer, pH 5.0, at 20°C

proof_lines: in the presence of 100 mM NaCl, in 50 mM acetate buffer, pH 5.0, at 20°C

proof_lines: at 21°C in 50 mM sodium phosphate buffer, pH 7.4

substrate: benzyl methyl sulfide


organism: Oryctolagus cuniculus

substrate_name: UTP; D-Glucose 1-phosphate

substrate: UTP; D-Glucose 1-phosphate


organism: Saccharomyces cerevisiae


substrate_name: D-Glucose 1-phosphate; UTP

substrate: D-Glucose 1-phosphate; UTP


enzyme_display_name: protein N-terminal methyltransferase

substrate_name: N-terminal-SPKRIAKRRSPP

proof_lines: recombinant His6-tagged enzyme, pH 7.5, 37°C

proof_lines: recombinant detagged enzyme, pH 7.5, 37°C

proof_lines: pH and temperature not specified in the publication

enzyme: protein N-terminal methyltransferase

substrate: N-terminal-SPKRIAKRRSPP


proof_lines:
about, mutant W136F, pH 7.5, 37°C
above, mutant W136F, pH 7.5, 37°C


substrate_name: N-terminal-WPKRIA

substrate: N-terminal-WPKRIA


organism: Cucumis sativus

substrate_name: Phosphoenolpyruvate; H2O; CO2

proof_lines: pH 8.0, 25°C, enzyme from roots grown in absence of Fe2+

proof_lines: pH 8.0, 25°C, enzyme from roots grown in presence of Fe2+

substrate: Phosphoenolpyruvate; H2O; CO2


organism: Methanothermus sociabilis


proof_lines:
pH 8, PEPC1
pH 8, PEPC2
pH 7.3, PEPC1


enzyme_display_name: carbonyl reductase (NADPH)

organism: Cavia porcellus

substrate_name: 4-Nitroacetophenone; NADPH

assay_conditions_summary: cofactor NADPH

enzyme: carbonyl reductase (NADPH)

substrate: 4-Nitroacetophenone; NADPH


organism: Gallus gallus

substrate_name: H+; NADPH; Pyridine-4-aldehyde

substrate: H+; NADPH; Pyridine-4-aldehyde


substrate_name: H+; NADPH; Isatin

substrate: H+; NADPH; Isatin


substrate_name: NADPH; Pyridine-4-aldehyde; H+

substrate: NADPH; Pyridine-4-aldehyde; H+


organism: Canis familiaris

substrate_name: Palmitoyl-CoA; L-Carnitine

assay_conditions_summary: cofactor CoA

substrate: Palmitoyl-CoA; L-Carnitine


enzyme_display_name: non-specific serine/threonine protein kinase


enzyme_display_name: alcohol dehydrogenase (NADP+)

organism: Clostridium acetobutylicum

substrate_name: NADP+; 1-Butanol

assay_conditions_summary: cofactor NADP+

enzyme: alcohol dehydrogenase (NADP+)

substrate: NADP+; 1-Butanol


substrate_name: H+; D-Glucuronate; NADPH

substrate: H+; D-Glucuronate; NADPH


organism: Lactococcus lactis

substrate_name: H+; NADH; 2-Methylpropanal

assay_conditions_summary: cofactor NADH

substrate: H+; NADH; 2-Methylpropanal


substrate_name: NADPH; p-Nitrobenzaldehyde; H+

substrate: NADPH; p-Nitrobenzaldehyde; H+


substrate_name: NADPH; Acetaldehyde; H+

substrate: NADPH; Acetaldehyde; H+


enzyme_display_name: thymidine kinase

organism: Human herpesvirus 1

substrate_name: ATP; Thymidine

enzyme: thymidine kinase

substrate: ATP; Thymidine


organism: Mus musculus


substrate_name: ATP; 1-beta-D-Arabinofuranosylthymine

substrate: ATP; 1-beta-D-Arabinofuranosylthymine


organism: Vaccinia virus

substrate_name: VVTK; Deoxythymidine

substrate: VVTK; Deoxythymidine


organism: Arabidopsis thaliana

substrate_name: CRCK1; Calmodulin

enzyme: non-specific serine/threonine protein kinase

substrate: CRCK1; Calmodulin


organism: Danio rerio

substrate_name: ATP; Protein

substrate: ATP; Protein


organism: Opisthokonta

substrate_name: cdc2k; ATP

substrate: cdc2k; ATP


organism: Acer pseudoplatanus

substrate_name: Diphosphate; UDPglucose

substrate: Diphosphate; UDPglucose


organism: Felis silvestris catus

substrate_name: D-Glucose 1-phosphate; UTP

substrate: D-Glucose 1-phosphate; UTP


enzyme_display_name: granzyme B

organism: Homo sapiens

proof_lines:
pH 7.4, 37°C, wild-type
wild-type, 37°C

enzyme: granzyme B


organism: Dioscorea opposita

substrate_name: H2O; Glycolchitin

substrate: H2O; Glycolchitin


organism: Pagrus major

substrate_name: Glycolchitin; H2O

substrate: Glycolchitin; H2O


organism: Todarodes pacificus


substrate_name: Boc-Ala-Ala-Asp-SBzl

substrate: Boc-Ala-Ala-Asp-SBzl


proof_lines:
pH 7.5, 23°C
mouse


enzyme_display_name: ADAMTS13 endopeptidase

substrate_name: VWF73 peptide

proof_lines: pH 6.0, 37°C, recombinant G662A mutant ADAMTS13

enzyme: ADAMTS13 endopeptidase

substrate: VWF73 peptide


mutation_type: deletion

proof_lines: pH 6.0, 37°C, recombinant ADAMTS13 6-amino-acid-deletion mutant


proof_lines: pH 6.0, 37°C, recombinant Y661A mutant ADAMTS13


proof_lines: pH 6.0, 37°C, recombinant R659A mutant ADAMTS13


proof_lines: pH 6.0, 37°C, recombinant E664A mutant ADAMTS13


proof_lines: pH 6.0, 37°C, recombinant E663A mutant ADAMTS13


proof_lines: pH 6.0, 37°C, recombinant R660A mutant ADAMTS13


proof_lines: mutant R568K/F592Y/R660K/Y661F/Y665F, pH not specified in the publication, temperature not specified in the publication


substrate_name: von Willebrand factor 115

proof_lines:
recombinant enzyme, at 37°C
pH 7.5, 25°C, recombinant enzyme
pH 7.5, 37°C, recombinant ADAMTS13

substrate: von Willebrand factor 115


substrate_name: FRET-VWF115 peptide

substrate: FRET-VWF115 peptide


proof_lines: wild type enzyme, in 20 mM Tris-HCl (pH 7.8), 150 mM NaCl, 5 mM CaCl2, at 37°C

proof_lines: pH 7.8, 37°C, recombinant wild-type enzyme

proof_lines: pH 6.0, 37°C, recombinant wild-type ADAMTS13


enzyme_display_name: fructose-bisphosphate aldolase

organism: Bos taurus

substrate_name: D-Fructose 1,6-bisphosphate

enzyme: fructose-bisphosphate aldolase

substrate: D-Fructose 1,6-bisphosphate


organism: Bubalus bubalis


organism: Ceratitis capitata


organism: Chara foetida


enzyme_display_name: inorganic diphosphatase

organism: Spinacia oleracea

substrate_name: Diphosphate; H2O

enzyme: inorganic diphosphatase

substrate: Diphosphate; H2O


organism: Streptococcus gordonii


enzyme_display_name: phosphoenolpyruvate carboxylase

organism: Acholeplasma laidlawii

substrate_name: Phosphoenolpyruvate; H2O; CO2

enzyme: phosphoenolpyruvate carboxylase

substrate: Phosphoenolpyruvate; H2O; CO2


organism: Flaveria trinervia


organism: Triticum aestivum


organism: Helix pomatia


organism: Lactococcus lactis subsp. lactis


organism: Micrococcus aerogenes


organism: Cyprinus carpio


organism: Entamoeba histolytica


organism: Escherichia coli


organism: Galdieria sulphuraria


organism: Saccharomyces cerevisiae

substrate_name: beta-D-Fructose 1,6-bisphosphate

substrate: beta-D-Fructose 1,6-bisphosphate


organism: Scomber japonicus


organism: Spermophilus lateralis


organism: Staphylococcus aureus

substrate_name: D-Fructose 1-phosphate

substrate: D-Fructose 1-phosphate


organism: Sus scrofa


organism: Ustilago maydis


organism: Vigna radiata


enzyme_display_name: glucose-6-phosphate isomerase

organism: Archaeoglobus fulgidus

substrate_name: D-Glucose 6-phosphate

enzyme: glucose-6-phosphate isomerase

substrate: D-Glucose 6-phosphate


organism: Bacillus caldotenax


organism: Conger conger

substrate_name: D-Fructose 6-phosphate

substrate: D-Fructose 6-phosphate


organism: Daphnia magna


organism: Fundulus heteroclitus


organism: Ictalurus punctatus


organism: Lactobacillus casei


organism: Methanosarcina mazei


organism: Oryctolagus cuniculus


organism: Pagrus major


organism: Rattus norvegicus


organism: Ricinus communis


organism: Spinacia oleracea


organism: Trypanosoma cruzi


substrate_name: Glucose 6-phosphate

substrate: Glucose 6-phosphate


organism: Pisum sativum


organism: Saccharomyces cerevisiae

substrate_name: alpha-D-Glucose 6-phosphate

substrate: alpha-D-Glucose 6-phosphate


organism: Salmonella typhimurium


enzyme_display_name: pancreatic ribonuclease

organism: Bos taurus

substrate_name: Uridine monophosphate-uridine; H2O

proof_lines: binds to the active site of the enzyme by anchoring two molecules connected to each other by hydrogen bonds and van der Waals interactions

enzyme: pancreatic ribonuclease

substrate: Uridine monophosphate-uridine; H2O


organism: Equus caballus

substrate_name: 2',3'-Cyclic UMP; H2O

substrate: 2',3'-Cyclic UMP; H2O


organism: Gallus gallus

substrate_name: tRNA

assay_conditions_summary: pH 7

substrate: tRNA


organism: Homo sapiens

substrate_name: poly(A)poly(U)

substrate: poly(A)poly(U)


substrate_name: poly (C)

proof_lines: mutant R4A/K6A/Q9E/D16G/S17N, 25°C, pH 5.0

substrate: poly (C)


proof_lines:
wild-type, 25°C, pH 5.0
wild type enzyme


substrate_name: tRNA; H2O

substrate: tRNA; H2O


substrate_name: cyclic 2',3'-cytidine monophosphate

substrate: cyclic 2',3'-cytidine monophosphate


organism: Lithobates pipiens

substrate_name: Unknown

assay_conditions_summary: pH 6

substrate: Unknown


organism: Macaca fascicularis


organism: Pongo pygmaeus


organism: Salmo salar


organism: Sus scrofa


substrate_name: cytidine-2',3'-cyclic monophosphate

substrate: cytidine-2',3'-cyclic monophosphate


proof_lines:
wild-type, pH 5.5, 25°C
wild-type, pH 7.0
wild-type, pH7.0


proof_lines:
mutant K7H/R10H, pH 5.5, 25°C
mutant K7H/R10H, pH 7.0


substrate_name: pentacytidylic acid

substrate: pentacytidylic acid


substrate_name: poly(A)-poly(U)

substrate: poly(A)-poly(U)


substrate_name: poly(C)

substrate: poly(C)


proof_lines:
wild type enzyme, pH 5.5, 25°C
wild type and mutant enzyme


proof_lines:
wild-type, pH 7.5, 37°C
pH 7.5, 37°C, wild-type enzyme


substrate_name: RNA

proof_lines:
pH 7.0, 37°C
pH 5.5, 25ºC, comercial enzyme
pancreas

substrate: RNA


proof_lines:
PE3I1 variant, pH 5.5, 25ºC
PE3 variant, pH 5.5, 25ºC
PE3I2 variant, pH 5.5, 25ºC


substrate_name: cyclic 2',3'-CMP

substrate: cyclic 2',3'-CMP


organism: Balaenoptera physalus


substrate_name: H2O; 2',3'-Cyclic CMP

substrate: H2O; 2',3'-Cyclic CMP


substrate_name: SSKRAKAKTTKKRP

proof_lines: recombinant NRMT1, in presence of NRMT2 catalytically inactive V224L mutant, pH and temperature not specified in the publication

substrate: SSKRAKAKTTKKRP


substrate_name: S-adenosyl-L-methionine

proof_lines: with RCC1, pH and temperature not specified in the publication

proof_lines: with (E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine, pH and temperature not specified in the publication

proof_lines: with S-adenosyl-L-methionine, pH and temperature not specified in the publication

substrate: S-adenosyl-L-methionine


substrate_name: Ac-IEPD-p-nitroanilide

substrate: Ac-IEPD-p-nitroanilide


substrate_name: beta-glycan

substrate: beta-glycan


organism: Rattus norvegicus


proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


organism: Aotus trivirgatus


assay_conditions_summary: cofactor ATP


organism: Aquifex aeolicus

substrate_name: ATP

substrate: ATP


organism: Archaeoglobus fulgidus

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP


substrate_name: Peptide

substrate: Peptide


assay_conditions_summary: pH 7.4; 295.15 K; cofactor ATP


substrate_name: ATP; alpha-Casein

substrate: ATP; alpha-Casein


proof_lines: pH and temperature not specified in the publication


substrate_name: Lys-Lys-Phe-Asn-Arg-Thr-Leu-Ser-Val-Ala

substrate: Lys-Lys-Phe-Asn-Arg-Thr-Leu-Ser-Val-Ala


organism: Mycobacterium tuberculosis

substrate_name: EmbR; ATP

substrate: EmbR; ATP


substrate_name: ATP; Protein tyrosine

substrate: ATP; Protein tyrosine


enzyme_display_name: 4-hydroxyphenylpyruvate dioxygenase

organism: Mus musculus

substrate_name: 4-Hydroxyphenylpyruvate; O2

enzyme: 4-hydroxyphenylpyruvate dioxygenase

substrate: 4-Hydroxyphenylpyruvate; O2


organism: Picrophilus torridus


substrate_name: O2; 4-Hydroxyphenylpyruvate

substrate: O2; 4-Hydroxyphenylpyruvate


organism: Sphingobium sp.


organism: Streptomyces avermitilis


smiles: O=O


enzyme_display_name: phenylglycine N-acetyltransferase

organism: Chryseobacterium sp.

substrate_name: L-2-phenylglycine methyl ester

enzyme: phenylglycine N-acetyltransferase

substrate: L-2-phenylglycine methyl ester


organism: Chryseobacterium sp. 5-3B


organism: Arabidopsis thaliana


proof_lines: recombinant His-tagged enzyme, pH 7.0, 30°C

proof_lines: 30°C, pH not specified in the publication


organism: Homo sapiens


proof_lines: pH 7.0, 30°C, recombinant His-tagged enzyme


assay_conditions_summary: pH 7.5; 303.15 K; cofactor CoA


enzyme_display_name: tetraacyldisaccharide 4'-kinase

assay_conditions_summary: pH 8.5; 303.15 K; cofactor ATP

enzyme: tetraacyldisaccharide 4'-kinase


substrate_name: acetyl-CoA

substrate: acetyl-CoA


substrate_name: synthetic substrate

substrate: synthetic substrate


organism: Badhamia polycephala

substrate_name: actin-fragmin complex

substrate: actin-fragmin complex


organism: Bos taurus


organism: Caenorhabditis elegans

substrate_name: manganese/ATP

substrate: manganese/ATP


substrate_name: ATP (in the presence of 10 mM magnesium chloride)


substrate_name: manganese ions

substrate: manganese ions


substrate_name: phosphoenolpyruvate (at 32 degrees Celsius, pH 8.0)

assay_conditions_summary: cofactor ADP


substrate_name: ATP (for purified and in vitro activated AKT2)


substrate_name: ATP (for purified and in vitro activated AKT3)


substrate_name: ATP (for purified and in vitro activated AKT1)


substrate_name: myosin (isoform 2)

substrate: myosin (isoform 2)


proof_lines: pH 8.5, 30°C, recombinant wild-type enzyme


enzyme_display_name: lysine carboxypeptidase

substrate_name: fibrinopeptide alphaArg96-104

enzyme: lysine carboxypeptidase

substrate: fibrinopeptide alphaArg96-104


proof_lines: kcat is 2.0 sec(-1) with 3-(4-hydroxyphenyl)pyruvate as substrate.


substrate_name: ATP

proof_lines: Kinetics of autophosphorylation assay were measured, rather than phosphorylation of an exogenous substrate.


substrate_name: alpha-casein

proof_lines: Maximal velocity nearly identical for the reactions with alpha-casein and PER2 peptide.

substrate: alpha-casein


substrate_name: casein

substrate: casein


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: pNPP

proof_lines: kcat is 14.2 min(-1) for pNPP hydrolysis.

substrate: pNPP


organism: Plasmodium falciparum (isolate 3D7)

substrate_name: ATP (at 30 degrees Celsius)

proof_lines: kcat is 0.002 sec(-1) with ATP as substrate (at 30 degrees Celsius).

substrate: ATP (at 30 degrees Celsius)


organism: Plasmodium falciparum (isolate Dd2)

substrate_name: ATP (at pH 7.5 and 37 degrees Celsius)

assay_conditions_summary: pH 7.5; cofactor ATP

proof_lines: kcat is 1550 sec(-1) with ATP as substrate (at pH 7.5 and 37 degrees Celsius).

substrate: ATP (at pH 7.5 and 37 degrees Celsius)


organism: Tribolium castaneum

substrate_name: Drp1 (at 30 degrees Celsius)

proof_lines: kcat is 4.6 sec(-1) for phosphorylation of Drp1 (at pH 7.5 and 30 degrees Celsius) (PubMed:32484300). kcat is 20.9 sec(-1) for phosphorylation of ubiquitin (at pH 7.5 and 30 degrees Celsius) (PubMed:32484300). kcat is 18 min(-1) for phospho

substrate: Drp1 (at 30 degrees Celsius)


enzyme_display_name: ribonuclease III

organism: Escherichia coli

substrate_name: R1.1 RNA

assay_conditions_summary: pH 7.5; 310.15 K; buffer containing 10 mM Mg2+, mutant enzyme E65A

proof_lines: pH 7.5, 37°C, buffer containing 10 mM Mg2+, mutant enzyme E65A

enzyme: ribonuclease III

substrate: R1.1 RNA


assay_conditions_summary: pH 7.5; 310.15 K; buffer containing 10 mM Mg2+, mutant enzyme E100A

proof_lines: pH 7.5, 37°C, buffer containing 10 mM Mg2+, mutant enzyme E100A


assay_conditions_summary: pH 7.5; 310.15 K; buffer containing 10 mM Mg2+, mutant enzyme E38A

proof_lines: pH 7.5, 37°C, buffer containing 10 mM Mg2+, mutant enzyme E38A


assay_conditions_summary: pH 7.5; 310.15 K; buffer containing 10 mM Mg2+, mutant enzyme D114A

proof_lines: pH 7.5, 37°C, buffer containing 10 mM Mg2+, mutant enzyme D114A


assay_conditions_summary: pH 7.5; 310.15 K; buffer containing 10 mM Mg2+, wild-type enzyme

proof_lines: pH 7.5, 37°C, buffer containing 10 mM Mg2+, wild-type enzyme

proof_lines: recombinant wild-type enzyme, 37°C, in presence of 5 mM Mg2+

proof_lines: wild-type enzyme, pH 7.5, 37°C, in presence of Mg2+


assay_conditions_summary: pH 7.5; 310.15 K; buffer containing 10 mM Mg2+, mutant enzyme E41A

proof_lines: pH 7.5, 37°C, buffer containing 10 mM Mg2+, mutant enzyme E41A


substrate: ATP


enzyme_display_name: acyl-homoserine-lactone synthase

organism: Acinetobacter baumannii

substrate_name: Unknown

proof_lines: value calculated in silico, pH not specified in the publication, temperature not specified in the publication

enzyme: acyl-homoserine-lactone synthase

substrate: Unknown


organism: Aliivibrio fischeri

substrate_name: octanoyl-[acyl-carrier protein]

assay_conditions_summary: pH 8.5; 298.15 K; cofactor CoA

proof_lines: pH 8.5, 25°C, recombinant MBP-AinS, with octanoyl-[acyl-carrier-protein]

substrate: octanoyl-[acyl-carrier protein]


organism: Pseudomonas aeruginosa

substrate_name: butyryl-CoA

assay_conditions_summary: pH 8.8; 298.15 K; cofactor CoA

proof_lines:
pH 8.8, 25°C, recombinant enzyme
pH 7.8, 37°C, recombinant RhlI

substrate: butyryl-CoA


organism: Serratia fonticola

substrate_name: hexanoyl-[acyl-carrier protein]

assay_conditions_summary: cofactor CoA

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: hexanoyl-[acyl-carrier protein]


organism: Saccharomyces cerevisiae

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

proof_lines:
at pH 7.5 and 30°C
pH 7.5, 30°C


substrate_name: UDP-GlcNAc

substrate: UDP-GlcNAc


organism: Ceratitis capitata

substrate_name: methyl-alpha-D-mannoside

substrate: methyl-alpha-D-mannoside


substrate_name: methyl alpha-D-mannopyranoside

proof_lines: pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase

proof_lines: pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase

substrate: methyl alpha-D-mannopyranoside


organism: Rattus norvegicus


assay_conditions_summary: cofactor ATP

proof_lines: in vivo PDK1-activated mutant DELTA399-502/T389E (His6-S6K1aII(DELTA AID)-T389E)

proof_lines: in vitro PDK1-activated mutant DELTA399-502/T389E (His6-S6K1aII(DELTA AID)-T389E)


organism: Mus musculus

substrate_name: myelin basic protein

substrate: myelin basic protein


organism: Saccharolobus solfataricus

substrate_name: KKRAARATSNVFA

assay_conditions_summary: pH 6.5; 298.15 K; cofactor ATP

proof_lines: pH 6.5, 25°C, presence of poly(Glu4:Tyr)

substrate: KKRAARATSNVFA


proof_lines: recombinant enzyme mutant RNase III[DELTAdsRBD], 37°C, in presence of 5 mM Mn2+

proof_lines: recombinant enzyme mutant RNase III[DELTAdsRBD], 37°C, in presence of 25 mM Mg2+


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: poly(IC)

substrate: poly(IC)


organism: Aquifex aeolicus


organism: Drosophila melanogaster

substrate_name: 515 bp dsRNA

proof_lines: wild-type Dicer-2 in presence of Loquacious-PB at equimolar level, pH 7.4, 25°C

proof_lines: wild-type Dicer-2 in presence of protein R2D2 at equimolar level, pH 7.4, 25°C

substrate: 515 bp dsRNA


proof_lines: mutant E117D, pH 7.5, 37°C, in presence of Mn2+


substrate_name: RNA

proof_lines: recombinant His-tagged enzyme, pH 8.0, 35°C

substrate: RNA


organism: Homo sapiens

substrate_name: fibrinopeptide gammaLys54-62

substrate: fibrinopeptide gammaLys54-62


substrate_name: hippuryl-L-Arg

substrate: hippuryl-L-Arg


organism: Sus scrofa

substrate_name: Hippuryl-L-argininic acid

substrate: Hippuryl-L-argininic acid


organism: Echinochloa crus-galli

substrate_name: O2; 4-Hydroxyphenylpyruvate

substrate: O2; 4-Hydroxyphenylpyruvate


substrate_name: ATP; Protein

proof_lines: values for inhibition at the PH-domain of PKBalpha are obtained using competition with biotinylated diC8-phosphatidylinositol 3,4-bisphosphate

substrate: ATP; Protein


enzyme_display_name: alanine racemase

organism: Acidiphilium organovorum

substrate_name: D-Ala

assay_conditions_summary: pH 9

enzyme: alanine racemase

substrate: D-Ala


organism: Aquifex pyrophilus


organism: Bacillus anthracis

substrate_name: L-alanine

substrate: L-alanine


organism: Cellana grata


enzyme_display_name: non-specific serine/threonine protein kinase

organism: Danio rerio


organism: Oryctolagus cuniculus


proof_lines: kcat is 0.03 min(-1) for dsRNA consumption (PubMed:21419681). kcat is 0.03 min(-1) for dsRNA consumption (in the presence of r2d2) (PubMed:21419681). kcat is 0.03 min(-1) for dsRNA consumption (in the presence of loqs isoform PD) (PubMed:21


organism: Aeromonas hydrophila

substrate_name: butyryl-[acyl-carrier protein]

substrate: butyryl-[acyl-carrier protein]


organism: Mesorhizobium sp. ORS 3359

substrate_name: isobutanoyl-[ACP]

substrate: isobutanoyl-[ACP]


organism: Escherichia coli

substrate_name: R1.1 RNA

proof_lines: recombinant phosphorylated enzyme, pH 8.0, 37°C

proof_lines: recombinant non-phosphorylated enzyme, pH 8.0, 37°C

substrate: R1.1 RNA


organism: Saccharomyces cerevisiae


organism: Rattus norvegicus

substrate_name: eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) (in absence of DELE1 and in absence of hemin)


organism: Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

substrate_name: ATP during hexamerization

substrate: ATP during hexamerization


organism: Toxoplasma gondii

substrate_name: ATP (at pH 7.5 and 30 degrees Celsius)

substrate: ATP (at pH 7.5 and 30 degrees Celsius)


organism: Aquifex aeolicus (strain VF5)

substrate_name: 16S-u-hp RNA

substrate: 16S-u-hp RNA


organism: Homo sapiens

substrate_name: ATP (at pH 7.5)

substrate: ATP (at pH 7.5)


substrate_name: IDP

substrate: IDP


substrate_name: ATP

substrate: ATP


substrate_name: RRKDLHDDEEDEAMSIYpA peptide

substrate: RRKDLHDDEEDEAMSIYpA peptide


substrate_name: manganese/ATP

substrate: manganese/ATP


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: EmbR

substrate: EmbR


substrate_name: GarA

substrate: GarA


organism: Oryza sativa subsp. indica


organism: Plasmodium falciparum (isolate 3D7)

substrate_name: MTIP (at 30 degrees Celsius)

substrate: MTIP (at 30 degrees Celsius)


enzyme_display_name: glutathione-disulfide reductase

organism: Mus musculus

substrate_name: H+; NADPH; Oxidized glutathione

enzyme: glutathione-disulfide reductase

substrate: H+; NADPH; Oxidized glutathione


organism: Populus trichocarpa

substrate_name: NADPH

assay_conditions_summary: pH 7.6; 295.15 K; cofactor NADPH

substrate: NADPH


substrate_name: glutathione disulfide

substrate: glutathione disulfide


assay_conditions_summary: pH 7.6; 303.15 K; cofactor NADPH


substrate_name: histone H3 N6,N6,N6-trimethyl-L-lysine9

proof_lines: KDM4C, pH and temperature not specified in the publication

substrate: histone H3 N6,N6,N6-trimethyl-L-lysine9


substrate_name: histone H3 N6,N6-dimethyl-L-lysine9

proof_lines: KDM4B, pH and temperature not specified in the publication

substrate: histone H3 N6,N6-dimethyl-L-lysine9


proof_lines: KDM4A, pH and temperature not specified in the publication


proof_lines: KDM4D, pH and temperature not specified in the publication


proof_lines: KDM4E, pH and temperature not specified in the publication


substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication

substrate: Unknown


enzyme_display_name: biphenyl 2,3-dioxygenase

organism: Paraburkholderia xenovorans

substrate_name: biphenyl

proof_lines: mutant enzyme S283M, at 37°C, pH not specified in the publication

enzyme: biphenyl 2,3-dioxygenase

substrate: biphenyl


proof_lines: wild type enzyme, at 37°C, pH not specified in the publication


enzyme_display_name: alkane 1-monooxygenase

organism: Pseudomonas putida

enzyme: alkane 1-monooxygenase


enzyme_display_name: putidaredoxin--NAD(+) reductase

enzyme: putidaredoxin--NAD(+) reductase


substrate_name: oxidized 2,6-dichlorophenolindolphenol

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADH

proof_lines: mutant R310E, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol

substrate: oxidized 2,6-dichlorophenolindolphenol


proof_lines: mutant R65A, pH 7.5, 25°C, cosubstrate K3Fe(CN)6


substrate_name: NADH

assay_conditions_summary: pH 7.5; 277.15 K; cofactor NADH

proof_lines: mutant K409A, pH 7.5, 25°C, cosubstrate K3Fe(CN)6

substrate: NADH


proof_lines: mutant R310A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol


proof_lines: mutant R339A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol


proof_lines: mutant K387A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol


proof_lines: wild-type, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol


proof_lines: mutant N384A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol


organism: Allochromatium vinosum

substrate_name: NADH; H+; Glutathione disulfide

substrate: NADH; H+; Glutathione disulfide


assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADPH


substrate_name: NADP+

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADP+

substrate: NADP+


substrate_name: Oxidized glutathione

substrate: Oxidized glutathione


substrate_name: H+; NADPH; Glutathione disulfide

substrate: H+; NADPH; Glutathione disulfide


organism: Sus scrofa


organism: Taxus cuspidata


enzyme_display_name: 1,3-beta-oligoglucan phosphorylase

organism: Anaerolinea thermophila

substrate_name: D-glucose

enzyme: 1,3-beta-oligoglucan phosphorylase

substrate: D-glucose


organism: Euglena gracilis


organism: Euglena gracilis var. saccharophila


enzyme_display_name: 4,6-alpha-glucanotransferase (linear substrates/linear products)

organism: Limosilactobacillus reuteri

substrate_name: maltose

substrate: maltose


substrate_name: amylose V

proof_lines:
hydrolysis reaction, pH 5.0, 55°C
transfer reaction, pH 5.0, 55°C

enzyme: 4,6-alpha-glucanotransferase (linear substrates/linear products)

substrate: amylose V


substrate_name: amylose

proof_lines: mutant enzyme I1020M, at pH 5.0 and 40°C

substrate: amylose


proof_lines: mutant enzyme Q1126I, at pH 5.0 and 40°C


proof_lines: mutant enzyme S1057P, at pH 5.0 and 40°C


proof_lines:
wild type enzyme, at pH 5.0 and 40°C
pH 5.0, 40°C, wild-type enzyme


proof_lines: mutant enzyme H1056G, at pH 5.0 and 40°C


enzyme_display_name: reuteransucrase

enzyme: reuteransucrase


enzyme_display_name: [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase

proof_lines: pH 7.5, 30°C, enzyme construct alpha1beta1gamma1

proof_lines: pH 7.5, 30°C, enzyme construct alpha1beta2gamma1

proof_lines: pH 7.5, 30°C, enzyme construct alpha1beta2gamma3


enzyme_display_name: ROCK-subfamily protein kinase

substrate_name: [S6 peptide]-L-serine

proof_lines: purified 66 kDa kinase domain of ROCK-II, at pH 7.4 and 30°C

enzyme: ROCK-subfamily protein kinase

substrate: [S6 peptide]-L-serine


proof_lines: Ki below 0.000005 mM, pH and temperature not specified in the publication

proof_lines: Ki below 0.000001 mM, pH and temperature not specified in the publication


enzyme_display_name: [myosin heavy-chain] kinase

organism: Dictyostelium discoideum

substrate_name: RKKFGESEKTKTKEFL-amide

enzyme: [myosin heavy-chain] kinase

substrate: RKKFGESEKTKTKEFL-amide


proof_lines:
pH 7.0, 25°C
recombinant MHCK B, pH 7.0, 22°C


substrate_name: ATP

assay_conditions_summary: pH 7; 295.15 K; cofactor ATP

substrate: ATP