enzyme_display_name: Citrate synthase, mitochondrial

organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

enzyme: Citrate synthase, mitochondrial


organism: Homo sapiens

substrate_name: acetyl-CoA

source_db: uniprot

verification_status: verified

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

protein_accession_database: UniProt

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

assay_conditions_summary: cofactor CoA

column_label: Downloaded ScienceDirect/EJMG PDF page 414 Table 1 lists the wild-type row with Km (uM) (Acetyl-CoA)=87.33 +/- 15, and the adjacent Results prose states Km 87.3 +/- 15 uM.

normalized_value_unit_evidence: Km normalized_value=0.0873 normalized_unit=mM from the prose value 87.3 uM.

raw_value_unit_evidence: Results prose reports Km 87.3 +/- 15 uM for wild-type mHS acetyl-CoA; Table 1 wild-type row gives Km (uM) (Acetyl-CoA)=87.33 +/- 15.

row_label: wild-type human mitochondrial HMG-CoA synthase

table_label: PDF Table 1 and adjacent Results prose

data_origin: experimental

substrate: acetyl-CoA

status: verified

source: uniprot


enzyme_display_name: citrate (Si)-synthase

organism: Rattus norvegicus

substrate_name: oxaloacetate

source_db: oed

verification_status: unverified

kcat_over_km_origin: calculated

smiles: O=C(O)CC(=O)C(=O)O

enzyme: citrate (Si)-synthase

substrate: oxaloacetate

status: unverified

source: oed


enzyme_display_name: Name not preserved

organism: Mycobacterium tuberculosis

source_db: skid

source_license: CC BY 4.0; CC BY-NC-ND 4.0

sequence_source: multiple

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

assay_conditions_summary: pH 7.5; 298.15 K; cofactor CoA

source_databases_merged: skid uniprot

enzyme: Name not preserved

source: skid


substrate_name: glyoxylate

substrate: glyoxylate


enzyme_display_name: hydroxymethylglutaryl-CoA synthase

organism: Haloferax volcanii

assay_conditions_summary: pH 8; 303.15 K; cofactor CoA

column_label: Table 1; HvHMGCS row; Km (uM) / Acetyl-CoA, kcat (s^-1), and kcat/Km (s^-1 mM^-1) columns; printed cells 50 +/- 6 uM, 4.6 +/- 0.2 s^-1, and 92 +/- 11 s^-1 mM^-1. Footnote a states values are means and standard errors.

raw_value_unit_evidence: Table 1 reports HvHMGCS acetyl-CoA Km = 50 +/- 6 uM, kcat = 4.6 +/- 0.2 s^-1, and kcat/Km = 92 +/- 11 s^-1 mM^-1.

row_label: HvHMGCS

table_label: Table 1. Kinetic parameters of H. volcanii, E. faecalis, and H. sapiens HMGCS enzymes

enzyme: hydroxymethylglutaryl-CoA synthase


assay_conditions_summary: pH 8; cofactor CoA

column_label: Page 8 section Activity of the Recombinant HMG-CoA Synthase states the recombinant synthase yielded a Km for Ac-CoA of 29 +/- 7 uM.

raw_value_unit_evidence: Km raw_value=29 +/- 7 raw_unit=uM

row_label: recombinant wild-type human cytoplasmic HMG-CoA synthase with Ac-CoA

table_label: Activity of the Recombinant HMG-CoA Synthase section, page 8


enzyme_display_name: 2-methylcitrate synthase

organism: Escherichia coli (strain K12)

substrate_name: propionyl-CoA (at pH 7.4)

assay_conditions_summary: pH 7.4; cofactor CoA

enzyme: 2-methylcitrate synthase

substrate: propionyl-CoA (at pH 7.4)


organism: Gallus gallus

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


organism: Mus musculus


enzyme_display_name: malate synthase

organism: Geobacillus stearothermophilus

source_db: brenda

proof_lines:
Bacillus stearothermophilus
malate synthase
isocitrate lyase

enzyme: malate synthase

source: brenda


enzyme_display_name: sucrose phosphorylase

organism: Bifidobacterium dentium

substrate_name: alpha-D-glucose 1-phosphate

enzyme: sucrose phosphorylase

substrate: alpha-D-glucose 1-phosphate


substrate_name: D-fructose

substrate: D-fructose


enzyme_display_name: levansucrase

organism: Pantoea agglomerans

substrate_name: sucrose

enzyme: levansucrase

substrate: sucrose


enzyme_display_name: UniProt G7LSK3

organism: Brenneria sp. EniD312

source_license: CC BY-NC-ND 4.0

enzyme: UniProt G7LSK3


organism: Leuconostoc mesenteroides


organism: Paraburkholderia graminis


enzyme_display_name: sucrose synthase

organism: Solanum tuberosum

enzyme: sucrose synthase


substrate_name: D-Lyxose

substrate: D-Lyxose


substrate_name: D-Xylulose

substrate: D-Xylulose


substrate_name: L-Sorbose

substrate: L-Sorbose


substrate_name: UDP-D-galactose

substrate: UDP-D-galactose


substrate_name: UDP-D-glucose

substrate: UDP-D-glucose


enzyme_display_name: sucrose-phosphate synthase

organism: Ipomoea batatas

substrate_name: D-fructose 6-phosphate

enzyme: sucrose-phosphate synthase

substrate: D-fructose 6-phosphate


substrate_name: UDP-glucose

substrate: UDP-glucose


organism: Streptomyces arenae


organism: Euglena gracilis


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution

proof_lines:
mutant D159A
mutant D159A, acetyl-S-enzyme formation
mutant D159A, acetyl-CoA hydrolysis


proof_lines:
mutant D203A
mutant D203A, acetyl-S-enzyme formation


substrate_name: acetoacetyl-CoA

substrate: acetoacetyl-CoA


proof_lines:
mutant D99A
mutant D99A, acetyl-S-enzyme formation


organism: Escherichia coli


sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines:
wild-type, pH 8.0, 37°C
wild-type protein


assay_conditions_summary: pH 8; 310.15 K; cofactor CoA


substrate_name: Unknown

km_display: Not reported

ki_unit: mM

substrate: Unknown


organism: Aspergillus fumigatus

proof_lines: pH 8.0, temperature not specified in the publication, mutant enzyme G352A


assay_conditions_summary: pH 8


substrate_name: propanoyl-CoA

substrate: propanoyl-CoA


proof_lines: pH 8.0, temperature not specified in the publication, wild-type enzyme


substrate_name: acetyl-CoA

substrate: acetyl-CoA


proof_lines: pH 8.0, temperature not specified in the publication, substrate: propionyl-CoA

proof_lines: pH 8.0, temperature not specified in the publication, substrate: oxaloacetate


proof_lines:
wild-type protein
wild-type, pH 8.0, 37°C


assay_conditions_summary: pH 8; 298.15 K; cofactor CoA

proof_lines:
pH 8.0, 25°C, purified native enzyme
versus CoA


substrate_name: oxaloacetate

substrate: oxaloacetate


substrate_name: CoA

proof_lines: pH 8.0, 25°C, purified native enzyme, in absence of propionyl-CoA

proof_lines: pH 8.0, 25°C, purified native enzyme, in presence of propionyl-CoA

substrate: CoA


organism: Homo sapiens

mutation_type: deletion

proof_lines: comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A412 nm standard assay

proof_lines: comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A412 nm microplate assay


proof_lines: comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A300 nm standard assay


proof_lines:
mutant H264A, acetyl-CoA hydrolysis
mutant H264A


proof_lines:
mutant H264N, acetyl-CoA hydrolysis
mutant H264N


organism: Bacillus sp. (in: firmicutes)


substrate_name: glyoxylate

substrate: glyoxylate


substrate_name: propionyl-CoA

substrate: propionyl-CoA


organism: Fusarium solani


proof_lines: propionyl-CoA acetyl-CoA


organism: Hevea brasiliensis


organism: Mycobacterium tuberculosis


proof_lines: pH and temperature not specified in the publication


enzyme_display_name: ATP citrate synthase

organism: Rattus norvegicus

enzyme: ATP citrate synthase


substrate_name: phosphate

substrate: phosphate


organism: Arthrobacter sp.


organism: Bacillus anthracis


organism: Bos taurus


organism: Cupriavidus necator


organism: Enterococcus faecalis


organism: Gallus gallus

proof_lines:
acetyl-CoA hydrolysis, wild-type
wild-type, acetyl-CoA hydrolysis
wild-type, acetyl-S-enzyme formation


organism: Neurospora crassa

substrate_name: UDPglucose

substrate: UDPglucose


substrate_name: glucose 6-phosphate

substrate: glucose 6-phosphate


enzyme_display_name: UniProt O75795

enzyme: UniProt O75795


enzyme_display_name: UniProt P54855

substrate_name: testosterone

enzyme: UniProt P54855

substrate: testosterone


enzyme_display_name: UDP-glucuronosyltransferase 2B23

organism: Macaca fascicularis

substrate_name: androsterone

enzyme: UDP-glucuronosyltransferase 2B23

substrate: androsterone


substrate_name: estriol

substrate: estriol


enzyme_display_name: cellobiose phosphorylase

organism: Cellulomonas uda

substrate_name: D-glucoseamine

enzyme: cellobiose phosphorylase

substrate: D-glucoseamine


smiles: C(C(C(C(C(C=O)F)O)O)O)O


substrate_name: D-xylose

substrate: D-xylose


smiles: C(C=O)C(C(C(CO)O)O)O


smiles: C(C(C(C(C(C=O)O)O)O)O)F


organism: Acetivibrio thermocellus

substrate_name: alpha-D-glucose 1-phosphate

substrate: alpha-D-glucose 1-phosphate


organism: Methanosarcina barkeri


substrate_name: formylmethanofuran

substrate: formylmethanofuran


organism: Pseudomonas aeruginosa


enzyme_display_name: aminoglycoside 6'-N-acetyltransferase

organism: Moraxella sp.

substrate_name: tobramycin

enzyme: aminoglycoside 6'-N-acetyltransferase

substrate: tobramycin


substrate_name: kanamycin

substrate: kanamycin


substrate_name: acetyl-CoA

substrate: acetyl-CoA


enzyme_display_name: UniProt F2RH01

organism: Streptomyces venezuelae

enzyme: UniProt F2RH01


substrate_name: ADP-glucose

substrate: ADP-glucose


substrate_name: GDP-glucose

substrate: GDP-glucose


enzyme_display_name: UniProt P16442

organism: Homo sapiens

substrate_name: UDP-N-acetylgalactosamine

enzyme: UniProt P16442

substrate: UDP-N-acetylgalactosamine


substrate_name: UDP-galactose

proof_lines:
Raw SKiD Env: Temp=37°C, pH=7
Raw SKiD Env: Temp=-----, pH=-----

substrate: UDP-galactose


enzyme_display_name: UniProt P16422

substrate_name: UDP-alpha-D-galactose

enzyme: UniProt P16422

substrate: UDP-alpha-D-galactose


enzyme_display_name: UniProt P15291

substrate_name: chitotriose

enzyme: UniProt P15291

substrate: chitotriose


substrate_name: N-acetyl-beta-D-glucosamine

substrate: N-acetyl-beta-D-glucosamine


enzyme_display_name: hydroxymethylglutaryl-CoA synthase

proof_lines: mutant Y130F, hydrolysis partial reaction

enzyme: hydroxymethylglutaryl-CoA synthase


enzyme_display_name: malate synthase

organism: Pseudomonas putida

substrate_name: glyoxylate

enzyme: malate synthase

substrate: glyoxylate


substrate_name: Unknown

substrate: Unknown


organism: Rhodotorula toruloides

substrate_name: ATP

assay_conditions_summary: cofactor ATP

substrate: ATP


substrate_name: citrate

assay_conditions_summary: buffer containing 0

proof_lines: 0.2 M Tris-HCl buffer containing 0.25 M KCl

substrate: citrate


enzyme_display_name: UniProt Q10471

substrate_name: UDP-N-acetyl-alpha-D-galactosamine

proof_lines:
Raw SKiD Env: Temp=37°C, pH=7.4
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=37°C, pH=-----

enzyme: UniProt Q10471

substrate: UDP-N-acetyl-alpha-D-galactosamine


substrate_name: UDP-Gal

substrate: UDP-Gal


enzyme_display_name: UniProt Q8N4A0

substrate_name: UDP-GalNAc

enzyme: UniProt Q8N4A0

substrate: UDP-GalNAc


organism: Rattus norvegicus

assay_conditions_summary: pH 7.6; buffer Hepes; cofactor ATP

proof_lines: dephosphoenzyme in Tris-HCl buffer, pH 8.7


proof_lines:
phospho-form of enzyme
dephospho-form of enzyme


organism: Saccharomyces cerevisiae

substrate_name: Gly-Asn-Ala-Ala-Ser-Arg-Arg

substrate: Gly-Asn-Ala-Ala-Ser-Arg-Arg


substrate_name: Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg

substrate: Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg


enzyme_display_name: UniProt Q93HT8

substrate_name: phenyl beta-D-glucopyranoside

enzyme: UniProt Q93HT8

substrate: phenyl beta-D-glucopyranoside


substrate_name: acetyl-CoA

substrate: acetyl-CoA


enzyme_display_name: aminoglycoside 3-N-acetyltransferase

substrate_name: sisomycin

enzyme: aminoglycoside 3-N-acetyltransferase

substrate: sisomycin


substrate_name: kanamycin A

substrate: kanamycin A


organism: Mesocricetus auratus

proof_lines: pancreatic cancer cell line, distal colon


proof_lines:
distal colon
pancreatic cancer cell line


enzyme_display_name: steroid N-acetylglucosaminyltransferase

organism: Oryctolagus cuniculus

substrate_name: UDP-N-acetylglucosamine

enzyme: steroid N-acetylglucosaminyltransferase

substrate: UDP-N-acetylglucosamine


organism: Aquifex aeolicus

substrate_name: maltose

substrate: maltose


substrate_name: maltoheptaose

substrate: maltoheptaose


substrate_name: maltotetraose

substrate: maltotetraose


substrate_name: maltotriose

substrate: maltotriose


substrate_name: maltopentaose

substrate: maltopentaose


substrate_name: maltohexaose

substrate: maltohexaose


organism: Ipomoea batatas


enzyme_display_name: UniProt O87172

organism: Thermus thermophilus

enzyme: UniProt O87172


enzyme_display_name: UniProt Q2VJA0

organism: Thermus brockianus

enzyme: UniProt Q2VJA0


enzyme_display_name: UniProt Q8NNA7

organism: Corynebacterium glutamicum

enzyme: UniProt Q8NNA7


enzyme_display_name: UniProt Q9LV91

organism: Arabidopsis thaliana

assay_conditions_summary: pH 7

enzyme: UniProt Q9LV91


substrate_name: acetoacetyl-CoA

substrate: acetoacetyl-CoA


organism: Petunia axillaris

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

proof_lines:
degree of polymerization: 14
degree of polymerization: 11
degree of polymerization: 7


substrate_name: UDP-D-galacturonic acid

substrate: UDP-D-galacturonic acid


organism: Nicotiana tabacum


organism: Vigna angularis


enzyme_display_name: monogalactosyldiacylglycerol synthase

organism: Bifidobacterium bifidum

substrate_name: UDPgalactose

enzyme: monogalactosyldiacylglycerol synthase

substrate: UDPgalactose


organism: Vigna radiata var. radiata


substrate_name: UDP-GalNAc

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature Y302L with inferred residue-numbering offset +7 to reconstruct the assayed variant sequence.

substrate: UDP-GalNAc


organism: Homo sapiens

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature D458H with inferred residue-numbering offset -9 to reconstruct the assayed variant sequence.

proof_lines: +/-0.001 mM, mutant GalNAc-T2-D458H, pH7.4


substrate_name: UDP-Gal

substrate: UDP-Gal


substrate_name: FGF23 NAPIPRRHTRSAEDDS peptide

substrate: FGF23 NAPIPRRHTRSAEDDS peptide


organism: Taeniopygia guttata


organism: Spinacia oleracea

substrate_name: UDP-D-galactose; 1,2-Diacyl-sn-glycerol

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

substrate: UDP-D-galactose; 1,2-Diacyl-sn-glycerol


organism: Chlorobaculum tepidum

substrate_name: UDP-galactose

substrate: UDP-galactose


organism: Biomphalaria glabrata

substrate_name: Muc2 acceptor peptide (at pH 7 and 37 degrees Celsius)


organism: Drosophila melanogaster


enzyme_display_name: type 1 galactoside alpha-(1,2)-fucosyltransferase

organism: Escherichia coli

substrate_name: Galbeta-O-Me

substrate: Galbeta-O-Me


substrate_name: lactulose

enzyme: type 1 galactoside alpha-(1,2)-fucosyltransferase

substrate: lactulose


substrate_name: Unknown

substrate: Unknown


organism: Rattus norvegicus


enzyme_display_name: alpha,alpha-trehalose phosphorylase

organism: Caldanaerobacter subterraneus subsp. pacificus

substrate_name: alpha-D-arabinose

assay_conditions_summary: pH 6; 333.15 K; buffer 50 mM MES

enzyme: alpha,alpha-trehalose phosphorylase

substrate: alpha-D-arabinose


substrate_name: alpha-D-galactose

substrate: alpha-D-galactose


substrate_name: alpha-D-xylose

substrate: alpha-D-xylose


substrate_name: alpha-L-fucose

substrate: alpha-L-fucose


substrate_name: methyl beta-D-galactopyranoside

substrate: methyl beta-D-galactopyranoside


substrate_name: lactose

substrate: lactose


substrate_name: GDP-beta-L-fucose

substrate: GDP-beta-L-fucose


organism: Caldanaerobacter subterraneus

substrate_name: D-galactose

proof_lines: mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C

substrate: D-galactose


substrate_name: beta-D-galactose 1-phosphate

substrate: beta-D-galactose 1-phosphate


substrate_name: beta-D-glucose 1-phosphate

substrate: beta-D-glucose 1-phosphate


substrate_name: D-glucose

substrate: D-glucose


organism: Kocuria varians

substrate_name: trehalose

substrate: trehalose


substrate_name: phosphate

substrate: phosphate


substrate_name: glucose

substrate: glucose


enzyme_display_name: Histo-blood group ABO system transferase

substrate_name: type I H-antigen (A transferase)

proof_lines: kcat is 1.3 sec(-1) for type I H-antigen (A transferase) (PubMed:16326711). kcat is 0.5 sec(-1) for type I H-antigen (B transferase) (PubMed:16326711). kcat is 1.8 sec(-1) for type II H-antigen (A transferase) (PubMed:16326711). kcat is 1.2

substrate: type I H-antigen (A transferase)


substrate_name: IgA hinge

proof_lines: +/-0.005 mM, mutant GalNAc-T2-D458H, pH7.4

proof_lines: +/-0.006 mM, mutant GalNAc-T2-D458H, pH7.4

proof_lines: +/-0.19mM, mutant GalNAc-T2-D458H, pH7.4

substrate: IgA hinge


organism: Salisediminibacterium selenitireducens


substrate_name: alpha,alpha-trehalose

substrate: alpha,alpha-trehalose


organism: Helicobacter pylori

substrate_name: GDP-fucose

proof_lines: with Gal-beta-1,4beta-GlcNAc-O-(CH2)8CO2CH3, wild type, strain NCTC116639

substrate: GDP-fucose


proof_lines: cosubstrate: Galbeta1,3GlcNAc-O(CH2)8CO2CH3, full-length enzyme

proof_lines: with Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3, strain UA948


organism: Euglena gracilis


organism: Ajuga reptans


substrate_name: raffinose

substrate: raffinose


mutation_signature: truncated enzyme UA948(1-428) truncated enzyme UA948(1-428)

mutation_type: truncation


mutation_signature: truncated enzyme UA948(1-428) cosubstrate: Galbeta1 / truncated enzyme UA948(1-428)

proof_lines: cosubstrate: Galbeta1,3GlcNAc-O(CH2)8CO2CH3, truncated enzyme UA948(1-428)


organism: Lymnaea stagnalis


organism: Caenorhabditis elegans


substrate_name: Unknown

proof_lines:
inhibition of trehalose synthesis
inhibition of trehalose phosphorolysis

substrate: Unknown


enzyme_display_name: procollagen glucosyltransferase

organism: Gallus gallus

substrate_name: D-galactosylhydroxylysine

enzyme: procollagen glucosyltransferase

substrate: D-galactosylhydroxylysine


organism: Mangifera indica


substrate_name: UDP-glucose

proof_lines: at high Mn2+-concentration, 2 Mn2+ bound

substrate: UDP-glucose


organism: Bos taurus

substrate_name: Collagen

proof_lines: denatured citrate-soluble calf skin collagen

proof_lines: acetic acid-soluble collagen from alkali-treated calf arterial tissue

substrate: Collagen


organism: Thermoanaerobacter brockii

proof_lines: kcat is 110 sec(-1) for trehalose. kcat is 110 sec(-1) for phosphate. kcat is 140 sec(-1) for D-glucose. kcat is 140 sec(-1) for beta-D-glucose 1-phosphate.

source_databases_merged: brenda uniprot


substrate_name: GDP-beta-L-fucose

substrate: GDP-beta-L-fucose


organism: Homo sapiens

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature W124R to reconstruct the assayed variant sequence.

proof_lines: mutant W124R, with Fuc-alpha-1,2Gal-beta-1,4GlcNAc-sp-biotin


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature W124Y to reconstruct the assayed variant sequence.

proof_lines: mutant W124Y, with Fuc-alpha-1,2Gal-beta-1,4GlcNAc-sp-biotin


proof_lines: kcat is 1.70 min(-1) for the alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-D-GlcNAc (PubMed:9737989). kcat is 9.3 min(-1) for the beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine (PubMed:9737988).


assay_conditions_summary: pH 6; buffer 50 mM MES

proof_lines: mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C


substrate_name: D-galactose

proof_lines: mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C

substrate: D-galactose


proof_lines: mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C


proof_lines: mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C


organism: Cucurbita pepo

proof_lines:
hydrolysis
+ raffinose


substrate_name: melibiose

substrate: melibiose


proof_lines: kcat is 1.70 min(-1) for the alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-D-GlcNAc (PubMed:9737989). kcat is 9.3 min(-1) for the beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine (PubMed:9737988).


enzyme_display_name: Stachyose synthase

organism: Pisum sativum

substrate_name: galactinol

proof_lines: Vmax/KM ratio for galactosyl transfer from galactinol to stachyose is 9.5 fold higher compared with galactosyl transfer from one stachyose molecule to another.

enzyme: Stachyose synthase

substrate: galactinol


organism: Vigna angularis

proof_lines: with 1-alpha-D-galactosyl-myo-inositol at 1.1 mM

proof_lines: with galactosylononitol at nonsaturating level

proof_lines: with 1-alpha-D-galactosyl-myo-inositol at nonsaturating level


substrate_name: myo-inositol

proof_lines: with 10 mM 1-alpha-D-galactosyl-myo-inositol in the exchange reaction

substrate: myo-inositol


organism: Arabidopsis thaliana

proof_lines: pH 7.0, 25°C, recombinant His-tagged enzyme


substrate_name: GDP-L-fucose

proof_lines: wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37°C

substrate: GDP-L-fucose


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_ranked_match and applied mutation signature C35T with inferred residue-numbering offset +7 to reconstruct the assayed variant sequence.

proof_lines: Km above 0.3984 mM, isoform FUT1 mutant C35T, in 50 mM Tris-HCl (pH 7.2), at 37°C


substrate_name: alpha-D-galactosyl-D-ononitol

substrate: alpha-D-galactosyl-D-ononitol


enzyme_display_name: UniProt Q9WTS2

organism: Mus musculus

substrate_name: GDP-L-Fuc

enzyme: UniProt Q9WTS2

substrate: GDP-L-Fuc


substrate_name: phenyl-beta-D-galactoside

substrate: phenyl-beta-D-galactoside


proof_lines:
membrane-bound enzyme
recombinant LH3/GGT
calf skin collagen as acceptor


substrate_name: TDP-glucose

substrate: TDP-glucose


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature D112E with inferred residue-numbering offset -8 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature D336A to reconstruct the assayed variant sequence.

proof_lines: fucosyltransferase III mutant enzyme D336A


proof_lines: bound in purified alpha1-chain of chicken skin collagen, value based on actual amount of galactosylhydroxylysine-residues of alpha1-chain


substrate_name: phenyl-beta-D-Galactoside

substrate: phenyl-beta-D-Galactoside


organism: Rattus norvegicus

substrate_name: phenyl-beta-galactose

substrate: phenyl-beta-galactose


organism: Sus scrofa


organism: Staphylococcus aureus

substrate_name: poly(ribitol phosphate)

substrate: poly(ribitol phosphate)


organism: Staphylococcus aureus (strain COL)

substrate_name: WTA

substrate: WTA


substrate_name: UDP-N-acetylglucosamine

substrate: UDP-N-acetylglucosamine


verification_status: corrected

sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature A431T to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'A431T', acceptor column group GAL; column 'Km (mM)' = 447.6; column 'kcat (s^-1)' = 66.5; column 'kcat/Km (mM^-1 s^-1)' = 0.149. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 66.5 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 66.5 s^(-1). Km: printed Table 2 cell 447.6 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 447.6 mM. kcat/Km: printed Table 2 cell 0.149 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.149 s^(-1)*mM^(-1). The exported values are kcat 66.5 s^(-1), Km 447.6 mM and kcat_over_km 0.14857015192135833 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'A431T', acceptor column group GAL: Km = 447.6 mM; kcat = 66.5 s-1; printed kcat/Km = 0.149 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.

table_label: Table 2: Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes (Biotechnol. Prog., 2012, Vol. 28, No. 5, pages 1257-1262). Printed column heads: Km (mM); kcat (s^-1); kcat/Km (mM^-1 s^-1); each metric head prints one value column under the acceptor group head GLC and one under GAL.

status: corrected


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature A440E to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'A440E', acceptor column group GAL; column 'Km (mM)' = 68.2; column 'kcat (s^-1)' = 23.0; column 'kcat/Km (mM^-1 s^-1)' = 0.337. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 23.0 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 23 s^(-1). Km: printed Table 2 cell 68.2 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 68.2 mM. kcat/Km: printed Table 2 cell 0.337 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.337 s^(-1)*mM^(-1). The exported values are kcat 23.0 s^(-1), Km 68.2 mM and kcat_over_km 0.33724340175953077 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'A440E', acceptor column group GAL: Km = 68.2 mM; kcat = 23.0 s-1; printed kcat/Km = 0.337 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature A440K to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'A440K', acceptor column group GAL; column 'Km (mM)' = 56.8; column 'kcat (s^-1)' = 27.7; column 'kcat/Km (mM^-1 s^-1)' = 0.488. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 27.7 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 27.7 s^(-1). Km: printed Table 2 cell 56.8 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 56.8 mM. kcat/Km: printed Table 2 cell 0.488 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.488 s^(-1)*mM^(-1). The exported values are kcat 27.7 s^(-1), Km 56.8 mM and kcat_over_km 0.4876760563380282 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'A440K', acceptor column group GAL: Km = 56.8 mM; kcat = 27.7 s-1; printed kcat/Km = 0.488 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature A440M to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'A440M', acceptor column group GAL; column 'Km (mM)' = 43.8; column 'kcat (s^-1)' = 17.0; column 'kcat/Km (mM^-1 s^-1)' = 0.388. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 17.0 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 17 s^(-1). Km: printed Table 2 cell 43.8 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 43.8 mM. kcat/Km: printed Table 2 cell 0.388 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.388 s^(-1)*mM^(-1). The exported values are kcat 17.0 s^(-1), Km 43.8 mM and kcat_over_km 0.3881278538812786 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'A440M', acceptor column group GAL: Km = 43.8 mM; kcat = 17.0 s-1; printed kcat/Km = 0.388 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature A440V to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'A440V', acceptor column group GAL; column 'Km (mM)' = 45.7; column 'kcat (s^-1)' = 32.9; column 'kcat/Km (mM^-1 s^-1)' = 0.720. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 32.9 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 32.9 s^(-1). Km: printed Table 2 cell 45.7 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 45.7 mM. kcat/Km: printed Table 2 cell 0.720 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.72 s^(-1)*mM^(-1). The exported values are kcat 32.9 s^(-1), Km 45.7 mM and kcat_over_km 0.7199124726477023 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'A440V', acceptor column group GAL: Km = 45.7 mM; kcat = 32.9 s-1; printed kcat/Km = 0.720 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature A440V/N657Y to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'A440V + N657Y', acceptor column group GAL; column 'Km (mM)' = 35.1; column 'kcat (s^-1)' = 23.6; column 'kcat/Km (mM^-1 s^-1)' = 0.672. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 23.6 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 23.6 s^(-1). Km: printed Table 2 cell 35.1 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 35.1 mM. kcat/Km: printed Table 2 cell 0.672 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.672 s^(-1)*mM^(-1). The exported values are kcat 23.6 s^(-1), Km 35.1 mM and kcat_over_km 0.6723646723646723 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'A440V + N657Y', acceptor column group GAL: Km = 35.1 mM; kcat = 23.6 s-1; printed kcat/Km = 0.672 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature A440V/R448S to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'A440V + R448S', acceptor column group GAL; column 'Km (mM)' = 30.1; column 'kcat (s^-1)' = 38.9; column 'kcat/Km (mM^-1 s^-1)' = 1.29. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 38.9 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 38.9 s^(-1). Km: printed Table 2 cell 30.1 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 30.1 mM. kcat/Km: printed Table 2 cell 1.29 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 1.29 s^(-1)*mM^(-1). The exported values are kcat 38.9 s^(-1), Km 30.1 mM and kcat_over_km 1.2923588039867109 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'A440V + R448S', acceptor column group GAL: Km = 30.1 mM; kcat = 38.9 s-1; printed kcat/Km = 1.29 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature N657D to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657D', acceptor column group GAL; column 'Km (mM)' = 61.9; column 'kcat (s^-1)' = 35.5; column 'kcat/Km (mM^-1 s^-1)' = 0.574. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 35.5 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 35.5 s^(-1). Km: printed Table 2 cell 61.9 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 61.9 mM. kcat/Km: printed Table 2 cell 0.574 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.574 s^(-1)*mM^(-1). The exported values are kcat 35.5 s^(-1), Km 61.9 mM and kcat_over_km 0.5735056542810986 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657D', acceptor column group GAL: Km = 61.9 mM; kcat = 35.5 s-1; printed kcat/Km = 0.574 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature N657G to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657G', acceptor column group GAL; column 'Km (mM)' = 58.1; column 'kcat (s^-1)' = 24.0; column 'kcat/Km (mM^-1 s^-1)' = 0.413. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 24.0 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 24 s^(-1). Km: printed Table 2 cell 58.1 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 58.1 mM. kcat/Km: printed Table 2 cell 0.413 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.413 s^(-1)*mM^(-1). The exported values are kcat 24.0 s^(-1), Km 58.1 mM and kcat_over_km 0.41308089500860584 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657G', acceptor column group GAL: Km = 58.1 mM; kcat = 24.0 s-1; printed kcat/Km = 0.413 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature N657I to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657I', acceptor column group GAL; column 'Km (mM)' = 53.5; column 'kcat (s^-1)' = 42.2; column 'kcat/Km (mM^-1 s^-1)' = 0.789. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 42.2 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 42.2 s^(-1). Km: printed Table 2 cell 53.5 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 53.5 mM. kcat/Km: printed Table 2 cell 0.789 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.789 s^(-1)*mM^(-1). The exported values are kcat 42.2 s^(-1), Km 53.5 mM and kcat_over_km 0.788785046728972 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657I', acceptor column group GAL: Km = 53.5 mM; kcat = 42.2 s-1; printed kcat/Km = 0.789 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


substrate_name: D-ononitol

substrate: D-ononitol


substrate_name: Unknown

substrate: Unknown


proof_lines: isoform FUT1 mutant C35T, in 50 mM Tris-HCl (pH 7.2), at 37°C


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'WT', acceptor column group GAL; column 'Km (mM)' = 98.0; column 'kcat (s^-1)' = 32.9; column 'kcat/Km (mM^-1 s^-1)' = 0.336. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 32.9 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 32.9 s^(-1). Km: printed Table 2 cell 98.0 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 98 mM. kcat/Km: printed Table 2 cell 0.336 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.336 s^(-1)*mM^(-1). The exported values are kcat 32.9 s^(-1), Km 98.0 mM and kcat_over_km 0.3357142857142857 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'WT', acceptor column group GAL: Km = 98.0 mM; kcat = 32.9 s-1; printed kcat/Km = 0.336 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


substrate_name: D-glucose

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'A431T', acceptor column group GLC; column 'Km (mM)' = 36.8; column 'kcat (s^-1)' = 32.1; column 'kcat/Km (mM^-1 s^-1)' = 0.872. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 32.1 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 32.1 s^(-1). Km: printed Table 2 cell 36.8 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 36.8 mM. kcat/Km: printed Table 2 cell 0.872 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.872 s^(-1)*mM^(-1). The exported values are kcat 32.1 s^(-1), Km 36.8 mM and kcat_over_km 0.8722826086956523 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'A431T', acceptor column group GLC: Km = 36.8 mM; kcat = 32.1 s-1; printed kcat/Km = 0.872 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.

substrate: D-glucose


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'A440E', acceptor column group GLC; column 'Km (mM)' = 2.3; column 'kcat (s^-1)' = 32.7; column 'kcat/Km (mM^-1 s^-1)' = 14. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 32.7 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 32.7 s^(-1). Km: printed Table 2 cell 2.3 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 2.3 mM. kcat/Km: printed Table 2 cell 14 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 14 s^(-1)*mM^(-1). The exported values are kcat 32.7 s^(-1), Km 2.3 mM and kcat_over_km 14.217391304347828 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'A440E', acceptor column group GLC: Km = 2.3 mM; kcat = 32.7 s-1; printed kcat/Km = 14 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'A440K', acceptor column group GLC; column 'Km (mM)' = 2.3; column 'kcat (s^-1)' = 41.2; column 'kcat/Km (mM^-1 s^-1)' = 18. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 41.2 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 41.2 s^(-1). Km: printed Table 2 cell 2.3 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 2.3 mM. kcat/Km: printed Table 2 cell 18 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 18 s^(-1)*mM^(-1). The exported values are kcat 41.2 s^(-1), Km 2.3 mM and kcat_over_km 17.91304347826087 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'A440K', acceptor column group GLC: Km = 2.3 mM; kcat = 41.2 s-1; printed kcat/Km = 18 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'A440M', acceptor column group GLC; column 'Km (mM)' = 2.1; column 'kcat (s^-1)' = 27.4; column 'kcat/Km (mM^-1 s^-1)' = 13. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 27.4 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 27.4 s^(-1). Km: printed Table 2 cell 2.1 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 2.1 mM. kcat/Km: printed Table 2 cell 13 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 13 s^(-1)*mM^(-1). The exported values are kcat 27.4 s^(-1), Km 2.1 mM and kcat_over_km 13.047619047619046 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'A440M', acceptor column group GLC: Km = 2.1 mM; kcat = 27.4 s-1; printed kcat/Km = 13 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'A440V', acceptor column group GLC; column 'Km (mM)' = 1.9; column 'kcat (s^-1)' = 42.0; column 'kcat/Km (mM^-1 s^-1)' = 22. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 42.0 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 42 s^(-1). Km: printed Table 2 cell 1.9 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 1.9 mM. kcat/Km: printed Table 2 cell 22 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 22 s^(-1)*mM^(-1). The exported values are kcat 42.0 s^(-1), Km 1.9 mM and kcat_over_km 22.105263157894736 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'A440V', acceptor column group GLC: Km = 1.9 mM; kcat = 42.0 s-1; printed kcat/Km = 22 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'A440V + N657Y', acceptor column group GLC; column 'Km (mM)' = 0.6; column 'kcat (s^-1)' = 16.1; column 'kcat/Km (mM^-1 s^-1)' = 27. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 16.1 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 16.1 s^(-1). Km: printed Table 2 cell 0.6 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 0.6 mM. kcat/Km: printed Table 2 cell 27 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 27 s^(-1)*mM^(-1). The exported values are kcat 16.1 s^(-1), Km 0.6 mM and kcat_over_km 26.833333333333336 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'A440V + N657Y', acceptor column group GLC: Km = 0.6 mM; kcat = 16.1 s-1; printed kcat/Km = 27 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'A440V + R448S', acceptor column group GLC; column 'Km (mM)' = 0.7; column 'kcat (s^-1)' = 25.0; column 'kcat/Km (mM^-1 s^-1)' = 36. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 25.0 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 25 s^(-1). Km: printed Table 2 cell 0.7 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 0.7 mM. kcat/Km: printed Table 2 cell 36 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 36 s^(-1)*mM^(-1). The exported values are kcat 25.0 s^(-1), Km 0.7 mM and kcat_over_km 35.714285714285715 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'A440V + R448S', acceptor column group GLC: Km = 0.7 mM; kcat = 25.0 s-1; printed kcat/Km = 36 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657D', acceptor column group GLC; column 'Km (mM)' = 1.8; column 'kcat (s^-1)' = 51.2; column 'kcat/Km (mM^-1 s^-1)' = 28. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 51.2 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 51.2 s^(-1). Km: printed Table 2 cell 1.8 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 1.8 mM. kcat/Km: printed Table 2 cell 28 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 28 s^(-1)*mM^(-1). The exported values are kcat 51.2 s^(-1), Km 1.8 mM and kcat_over_km 28.444444444444446 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657D', acceptor column group GLC: Km = 1.8 mM; kcat = 51.2 s-1; printed kcat/Km = 28 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657G', acceptor column group GLC; column 'Km (mM)' = 2.5; column 'kcat (s^-1)' = 42.7; column 'kcat/Km (mM^-1 s^-1)' = 17. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 42.7 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 42.7 s^(-1). Km: printed Table 2 cell 2.5 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 2.5 mM. kcat/Km: printed Table 2 cell 17 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 17 s^(-1)*mM^(-1). The exported values are kcat 42.7 s^(-1), Km 2.5 mM and kcat_over_km 17.080000000000002 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657G', acceptor column group GLC: Km = 2.5 mM; kcat = 42.7 s-1; printed kcat/Km = 17 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature R448S with inferred residue-numbering offset +24 to reconstruct the assayed variant sequence.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'WT', acceptor column group GLC; column 'Km (mM)' = 3.2; column 'kcat (s^-1)' = 33.9; column 'kcat/Km (mM^-1 s^-1)' = 11. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 33.9 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 33.9 s^(-1). Km: printed Table 2 cell 3.2 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 3.2 mM. kcat/Km: printed Table 2 cell 11 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 11 s^(-1)*mM^(-1). The exported values are kcat 33.9 s^(-1), Km 3.2 mM and kcat_over_km 10.593749999999998 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'WT', acceptor column group GLC: Km = 3.2 mM; kcat = 33.9 s-1; printed kcat/Km = 11 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


enzyme_display_name: UniProt P14769

substrate_name: p-nitrophenyl-beta-D-galactoside

enzyme: UniProt P14769

substrate: p-nitrophenyl-beta-D-galactoside


substrate_name: UDP-N-acetylgalactosamine

substrate: UDP-N-acetylgalactosamine


substrate_name: N-acetyllactosamine

proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=30°C, pH=6

substrate: N-acetyllactosamine


substrate_name: D-galactose

substrate: D-galactose


substrate_name: lactose

proof_lines:
Raw SKiD Env: Temp=37°C, pH=-----
Raw SKiD Env: Temp=30°C, pH=6
Raw SKiD Env: Temp=37°C, pH=7

substrate: lactose


substrate_name: UDP-galactose

proof_lines:
Raw SKiD Env: Temp=37°C, pH=-----
Raw SKiD Env: Temp=-----, pH=-----

substrate: UDP-galactose


sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

column_label: Table III columns GalNAc-T13-V1 Vmax and Km; row Muc5AC-3; Km (uM) column = 35+/-6.

raw_value_unit_evidence: Table III row Muc5AC-3, GalNAc-T13-V1 Km = 35+/-6 uM.

row_label: Muc5AC-3; GalNAc-T13-V1

table_label: Table III: Kinetic parameters for peptide glycosylation by GalNAc-T13 splice variants


column_label: Table III columns GalNAc-T13-V1 Vmax and Km; row Muc5AC-13; Km (uM) column = 130+/-15.

raw_value_unit_evidence: Table III row Muc5AC-13, GalNAc-T13-V1 Km = 130+/-15 uM.

row_label: Muc5AC-13; GalNAc-T13-V1


column_label: Table II columns GalNAc-T16 Vmax (pmol/h) and Km (uM); row Muc5AC-3.

normalized_value_unit_evidence: Actual primary Km is 0.299 mM; 310 is Vmax, not Km.

raw_value_unit_evidence: Table II row Muc5AC-3, GalNAc-T16 Vmax = 310+/-25 pmol/h and Km = 299+/-30 uM.

row_label: Muc5AC-3; GalNAc-T16

table_label: Table II: Kinetic parameters for peptide glycosylation by GalNAc-T1 and -T16


column_label: Table II columns GalNAc-T16 Vmax (pmol/h) and Km (uM); row Muc5AC-13.

normalized_value_unit_evidence: Actual primary Km is 0.115 mM; 390 is Vmax, not Km.

raw_value_unit_evidence: Table II row Muc5AC-13, GalNAc-T16 Vmax = 390+/-32 pmol/h and Km = 115+/-10 uM.

row_label: Muc5AC-13; GalNAc-T16


column_label: Table II columns GalNAc-T16 Vmax (pmol/h) and Km (uM); row EA2.

normalized_value_unit_evidence: Actual primary Km is 0.172 mM; 332 is Vmax, not Km.

raw_value_unit_evidence: Table II row EA2, GalNAc-T16 Vmax = 332+/-37 pmol/h and Km = 172+/-18 uM.

row_label: EA2; GalNAc-T16


substrate_name: ganglioside GA1

substrate: ganglioside GA1


substrate_name: ganglioside GM1a

substrate: ganglioside GM1a


proof_lines:
GA1 (Gg4Cer)
FGA1 (fucosyl GA1)
GM1 (II3NeuAc-Gg4Cer)


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature L310A with inferred residue-numbering offset +11 to reconstruct the assayed variant sequence.


substrate_name: UDP-N-acetyl-alpha-D-galactosamine

substrate: UDP-N-acetyl-alpha-D-galactosamine


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature N657L to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657L', acceptor column group GAL; column 'Km (mM)' = 61.1; column 'kcat (s^-1)' = 33.3; column 'kcat/Km (mM^-1 s^-1)' = 0.545. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 33.3 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 33.3 s^(-1). Km: printed Table 2 cell 61.1 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 61.1 mM. kcat/Km: printed Table 2 cell 0.545 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.545 s^(-1)*mM^(-1). The exported values are kcat 33.3 s^(-1), Km 61.1 mM and kcat_over_km 0.5450081833060556 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657L', acceptor column group GAL: Km = 61.1 mM; kcat = 33.3 s-1; printed kcat/Km = 0.545 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature N657R to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657R', acceptor column group GAL; column 'Km (mM)' = 62.5; column 'kcat (s^-1)' = 38.3; column 'kcat/Km (mM^-1 s^-1)' = 0.613. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 38.3 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 38.3 s^(-1). Km: printed Table 2 cell 62.5 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 62.5 mM. kcat/Km: printed Table 2 cell 0.613 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.613 s^(-1)*mM^(-1). The exported values are kcat 38.3 s^(-1), Km 62.5 mM and kcat_over_km 0.6127999999999999 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657R', acceptor column group GAL: Km = 62.5 mM; kcat = 38.3 s-1; printed kcat/Km = 0.613 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature N657Y to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657Y', acceptor column group GAL; column 'Km (mM)' = 45.8; column 'kcat (s^-1)' = 23.3; column 'kcat/Km (mM^-1 s^-1)' = 0.509. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 23.3 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 23.3 s^(-1). Km: printed Table 2 cell 45.8 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 45.8 mM. kcat/Km: printed Table 2 cell 0.509 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.509 s^(-1)*mM^(-1). The exported values are kcat 23.3 s^(-1), Km 45.8 mM and kcat_over_km 0.5087336244541485 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657Y', acceptor column group GAL: Km = 45.8 mM; kcat = 23.3 s-1; printed kcat/Km = 0.509 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature P588H to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'P588H', acceptor column group GAL; column 'Km (mM)' = 96.0; column 'kcat (s^-1)' = 54.3; column 'kcat/Km (mM^-1 s^-1)' = 0.566. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 54.3 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 54.3 s^(-1). Km: printed Table 2 cell 96.0 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 96 mM. kcat/Km: printed Table 2 cell 0.566 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.566 s^(-1)*mM^(-1). The exported values are kcat 54.3 s^(-1), Km 96.0 mM and kcat_over_km 0.5656249999999999 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'P588H', acceptor column group GAL: Km = 96.0 mM; kcat = 54.3 s-1; printed kcat/Km = 0.566 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature R448D to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448D', acceptor column group GAL; column 'Km (mM)' = 47.5; column 'kcat (s^-1)' = 21.1; column 'kcat/Km (mM^-1 s^-1)' = 0.444. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 21.1 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 21.1 s^(-1). Km: printed Table 2 cell 47.5 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 47.5 mM. kcat/Km: printed Table 2 cell 0.444 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.444 s^(-1)*mM^(-1). The exported values are kcat 21.1 s^(-1), Km 47.5 mM and kcat_over_km 0.4442105263157895 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448D', acceptor column group GAL: Km = 47.5 mM; kcat = 21.1 s-1; printed kcat/Km = 0.444 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature R448F with inferred residue-numbering offset +24 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature R448N to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448N', acceptor column group GAL; column 'Km (mM)' = 53.6; column 'kcat (s^-1)' = 32.4; column 'kcat/Km (mM^-1 s^-1)' = 0.604. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 32.4 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 32.4 s^(-1). Km: printed Table 2 cell 53.6 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 53.6 mM. kcat/Km: printed Table 2 cell 0.604 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.604 s^(-1)*mM^(-1). The exported values are kcat 32.4 s^(-1), Km 53.6 mM and kcat_over_km 0.6044776119402985 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448N', acceptor column group GAL: Km = 53.6 mM; kcat = 32.4 s-1; printed kcat/Km = 0.604 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature R448S to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448S', acceptor column group GAL; column 'Km (mM)' = 33.5; column 'kcat (s^-1)' = 31.9; column 'kcat/Km (mM^-1 s^-1)' = 0.952. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 31.9 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 31.9 s^(-1). Km: printed Table 2 cell 33.5 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 33.5 mM. kcat/Km: printed Table 2 cell 0.952 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.952 s^(-1)*mM^(-1). The exported values are kcat 31.9 s^(-1), Km 3.25 mM and kcat_over_km 9.815384615384614 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448S', acceptor column group GAL: Km = 33.5 mM; kcat = 31.9 s-1; printed kcat/Km = 0.952 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature R448S/N657Y to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448S + N657Y', acceptor column group GAL; column 'Km (mM)' = 38.0; column 'kcat (s^-1)' = 14.0; column 'kcat/Km (mM^-1 s^-1)' = 0.368. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 14.0 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 14 s^(-1). Km: printed Table 2 cell 38.0 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 38 mM. kcat/Km: printed Table 2 cell 0.368 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.368 s^(-1)*mM^(-1). The exported values are kcat 14.0 s^(-1), Km 38.0 mM and kcat_over_km 0.3684210526315789 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448S + N657Y', acceptor column group GAL: Km = 38.0 mM; kcat = 14.0 s-1; printed kcat/Km = 0.368 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature R448V to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448V', acceptor column group GAL; column 'Km (mM)' = 43.7; column 'kcat (s^-1)' = 26.2; column 'kcat/Km (mM^-1 s^-1)' = 0.600. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 26.2 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 26.2 s^(-1). Km: printed Table 2 cell 43.7 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 43.7 mM. kcat/Km: printed Table 2 cell 0.600 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.6 s^(-1)*mM^(-1). The exported values are kcat 26.2 s^(-1), Km 43.7 mM and kcat_over_km 0.5995423340961098 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448V', acceptor column group GAL: Km = 43.7 mM; kcat = 26.2 s-1; printed kcat/Km = 0.600 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657I', acceptor column group GLC; column 'Km (mM)' = 2.1; column 'kcat (s^-1)' = 46.9; column 'kcat/Km (mM^-1 s^-1)' = 22. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 46.9 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 46.9 s^(-1). Km: printed Table 2 cell 2.1 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 2.1 mM. kcat/Km: printed Table 2 cell 22 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 22 s^(-1)*mM^(-1). The exported values are kcat 46.9 s^(-1), Km 2.1 mM and kcat_over_km 22.333333333333332 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657I', acceptor column group GLC: Km = 2.1 mM; kcat = 46.9 s-1; printed kcat/Km = 22 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657L', acceptor column group GLC; column 'Km (mM)' = 2.2; column 'kcat (s^-1)' = 43.3; column 'kcat/Km (mM^-1 s^-1)' = 20. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 43.3 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 43.3 s^(-1). Km: printed Table 2 cell 2.2 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 2.2 mM. kcat/Km: printed Table 2 cell 20 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 20 s^(-1)*mM^(-1). The exported values are kcat 43.3 s^(-1), Km 2.2 mM and kcat_over_km 19.68181818181818 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657L', acceptor column group GLC: Km = 2.2 mM; kcat = 43.3 s-1; printed kcat/Km = 20 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657R', acceptor column group GLC; column 'Km (mM)' = 1.3; column 'kcat (s^-1)' = 31.4; column 'kcat/Km (mM^-1 s^-1)' = 24. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 31.4 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 31.4 s^(-1). Km: printed Table 2 cell 1.3 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 1.3 mM. kcat/Km: printed Table 2 cell 24 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 24 s^(-1)*mM^(-1). The exported values are kcat 31.4 s^(-1), Km 1.3 mM and kcat_over_km 24.153846153846153 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657R', acceptor column group GLC: Km = 1.3 mM; kcat = 31.4 s-1; printed kcat/Km = 24 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657Y', acceptor column group GLC; column 'Km (mM)' = 3.0; column 'kcat (s^-1)' = 53.9; column 'kcat/Km (mM^-1 s^-1)' = 18. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 53.9 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 53.9 s^(-1). Km: printed Table 2 cell 3.0 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 3 mM. kcat/Km: printed Table 2 cell 18 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 18 s^(-1)*mM^(-1). The exported values are kcat 53.9 s^(-1), Km 3.0 mM and kcat_over_km 17.966666666666665 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657Y', acceptor column group GLC: Km = 3.0 mM; kcat = 53.9 s-1; printed kcat/Km = 18 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature P588H with inferred residue-numbering offset +5 to reconstruct the assayed variant sequence.

proof_lines:
Thermoanaerobacter brockii
trehalose phosphorylase
R448S


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448D', acceptor column group GLC; column 'Km (mM)' = 1.2; column 'kcat (s^-1)' = 18.6; column 'kcat/Km (mM^-1 s^-1)' = 16. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 18.6 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 18.6 s^(-1). Km: printed Table 2 cell 1.2 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 1.2 mM. kcat/Km: printed Table 2 cell 16 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 16 s^(-1)*mM^(-1). The exported values are kcat 18.6 s^(-1), Km 1.2 mM and kcat_over_km 15.500000000000002 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448D', acceptor column group GLC: Km = 1.2 mM; kcat = 18.6 s-1; printed kcat/Km = 16 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature R448F to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448F', acceptor column group GLC; column 'Km (mM)' = 1.2; column 'kcat (s^-1)' = 21.7; column 'kcat/Km (mM^-1 s^-1)' = 18. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 21.7 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 21.7 s^(-1). Km: printed Table 2 cell 1.2 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 1.2 mM. kcat/Km: printed Table 2 cell 18 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 18 s^(-1)*mM^(-1). The exported values are kcat 21.7 s^(-1), Km 1.2 mM and kcat_over_km 18.083333333333332 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448F', acceptor column group GLC: Km = 1.2 mM; kcat = 21.7 s-1; printed kcat/Km = 18 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448N', acceptor column group GLC; column 'Km (mM)' = 1.5; column 'kcat (s^-1)' = 30.6; column 'kcat/Km (mM^-1 s^-1)' = 20. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 30.6 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 30.6 s^(-1). Km: printed Table 2 cell 1.5 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 1.5 mM. kcat/Km: printed Table 2 cell 20 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 20 s^(-1)*mM^(-1). The exported values are kcat 30.6 s^(-1), Km 1.5 mM and kcat_over_km 20.400000000000002 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448N', acceptor column group GLC: Km = 1.5 mM; kcat = 30.6 s-1; printed kcat/Km = 20 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448S', acceptor column group GLC; column 'Km (mM)' = 1.5; column 'kcat (s^-1)' = 26.3; column 'kcat/Km (mM^-1 s^-1)' = 18. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 26.3 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 26.3 s^(-1). Km: printed Table 2 cell 1.5 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 1.5 mM. kcat/Km: printed Table 2 cell 18 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 18 s^(-1)*mM^(-1). The exported values are kcat 26.3 s^(-1), Km 1.5 mM and kcat_over_km 17.533333333333335 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448S', acceptor column group GLC: Km = 1.5 mM; kcat = 26.3 s-1; printed kcat/Km = 18 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448S + N657Y', acceptor column group GLC; column 'Km (mM)' = 0.6; column 'kcat (s^-1)' = 7.0; column 'kcat/Km (mM^-1 s^-1)' = 12. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 7.0 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 7 s^(-1). Km: printed Table 2 cell 0.6 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 0.6 mM. kcat/Km: printed Table 2 cell 12 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 12 s^(-1)*mM^(-1). The exported values are kcat 7.0 s^(-1), Km 0.6 mM and kcat_over_km 11.666666666666668 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448S + N657Y', acceptor column group GLC: Km = 0.6 mM; kcat = 7.0 s-1; printed kcat/Km = 12 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


substrate_name: galacto-N-biose

assay_conditions_summary: pH 6.1; cofactor ATP

column_label: Table II | row Se (fut2) | column Galacto-N-biose Km (uM) | 0.38

raw_value_unit_evidence: Table II, Se (fut2), Galacto-N-biose, Km (uM) = 0.38

row_label: Se (fut2)

table_label: Table II. Kinetic parameters of bovine recombinant alpha2-fucosyltransferases

substrate: galacto-N-biose


substrate_name: Galacto-N-biose

column_label: Table II | row H (fut1) | column Galacto-N-biose Km (uM) | 0.26

raw_value_unit_evidence: Table II, H (fut1), Galacto-N-biose, Km (uM) = 0.26

row_label: H (fut1)

substrate: Galacto-N-biose


column_label: Table II | row H (fut1) | column GM1 Km (uM) | 1.29

raw_value_unit_evidence: Table II, H (fut1), GM1, Km (uM) = 1.29


enzyme_display_name: flavonol 3-O-glucosyltransferase

organism: Marchantia emarginata

substrate_name: apigenin

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

enzyme: flavonol 3-O-glucosyltransferase

substrate: apigenin


substrate_name: isorhamnetin

substrate: isorhamnetin


substrate_name: quercetin

substrate: quercetin


substrate_name: myricetin

substrate: myricetin


substrate_name: kaempferol

substrate: kaempferol


substrate_name: luteolin

substrate: luteolin


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: GA1 MFUT-I MFUT-II


substrate_name: lacto-N-biose

assay_conditions_summary: pH 6

column_label: Figure 3C reports apparent Km = 3.6 mM for lacto-N-biose I; Table I lists pcDNAI-alpha(1,2)FTSe lacto-N-biose I = 3.6 mM, This work.

raw_value_unit_evidence: Km raw_value=3.6 raw_unit=mM

row_label: Sec2-encoded alpha(1,2)fucosyltransferase; lacto-N-biose I

table_label: Figure 3 caption and Table I

substrate: lacto-N-biose


column_label: Figure 3D reports apparent Km = 3.8 mM for N-acetyllactosamine; Table I lists pcDNAI-alpha(1,2)FTSe N-acetyllactosamine = 3.8 mM, This work.

raw_value_unit_evidence: Km raw_value=3.8 raw_unit=mM


substrate_name: GDP-fucose

column_label: Figure 3E reports apparent Km = 197 microM for GDP-fucose; Table I lists pcDNAI-alpha(1,2)FTSe GDP-fucose = 0.197 mM, This work.

raw_value_unit_evidence: Km raw_value=197 raw_unit=microM

substrate: GDP-fucose


organism: Glycine max


substrate_name: fisetin

substrate: fisetin


substrate_name: UDPglucose

substrate: UDPglucose


organism: Raphanus sativus

substrate_name: 2,5-dihydroxy benzoate

proof_lines: pH 7.0, 30°C, recombinant wild-type enzyme

substrate: 2,5-dihydroxy benzoate


substrate_name: 2,4-dihydroxy benzoate

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

substrate: 2,4-dihydroxy benzoate


substrate_name: UDP-glucose

substrate: UDP-glucose


organism: Camellia sinensis


organism: Brassica oleracea


organism: Hippeastrum sp.


substrate_name: cyanidin

substrate: cyanidin


organism: Triticum aestivum


proof_lines:
reaction with 2,4,5-trichlorophenol
reaction with quercetin


substrate_name: trans-Resveratrol; UDP-D-glucose

substrate: trans-Resveratrol; UDP-D-glucose


substrate_name: UDP-D-glucose; trans-Resveratrol

substrate: UDP-D-glucose; trans-Resveratrol


organism: Stevia rebaudiana


organism: Cavia porcellus

substrate_name: UDP-N-acetylgalactosamine

substrate: UDP-N-acetylgalactosamine


substrate_name: globoside

substrate: globoside


enzyme_display_name: N-acetyllactosamine synthase

organism: Vigna radiata

enzyme: N-acetyllactosamine synthase


substrate_name: UDP-galactose

substrate: UDP-galactose


organism: Petroselinum crispum

substrate_name: quercetin

substrate: quercetin


organism: Prunus yedoensis


organism: Vigna mungo


substrate_name: UDP-D-glucose

substrate: UDP-D-glucose


organism: Vitis labrusca


substrate_name: UDP-alpha-D-glucose

proof_lines: using cyanidin as cosubstrate, in 100 mM Tris-HCl pH 8.0, at 30°C

substrate: UDP-alpha-D-glucose


organism: Paederia scandens

substrate_name: kaempferol

substrate: kaempferol


organism: Citrus x paradisi

assay_conditions_summary: pH 7.5; 303.15 K; buffer 50 mM phosphate

proof_lines: apparent value, in 50 mM phosphate buffer (pH 7.5), at 40°C


proof_lines: recombinant enzyme, in 50 mM HEPES, at pH 8.0 and 30°C


organism: Trichoplusia ni

substrate_name: p-nitrophenyl-GlcNAc

substrate: p-nitrophenyl-GlcNAc


assay_conditions_summary: pH 7.5; buffer 50 mM phosphate


substrate_name: myricetin

substrate: myricetin


organism: Bos taurus

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature E317Q with inferred residue-numbering offset -6 to reconstruct the assayed variant sequence.

proof_lines: 37°C, mutant enzyme E317Q, hydrolase reaction

proof_lines: 37°C, mutant enzyme E317Q, galactosyltransferase reaction


substrate_name: lactose

substrate: lactose


organism: Canis lupus familiaris


substrate_name: N-Acetylglucosamine; UDPgalactose

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

source_databases_merged: sabio_rk skid uniprot

substrate: N-Acetylglucosamine; UDPgalactose


enzyme_display_name: Beta-1,4-galactosyltransferase 4

organism: Homo sapiens

substrate_name: GlcNAc-B-S-pNP

enzyme: Beta-1,4-galactosyltransferase 4

substrate: GlcNAc-B-S-pNP


enzyme_display_name: Beta-1,4-galactosyltransferase 3

enzyme: Beta-1,4-galactosyltransferase 3


enzyme_display_name: Beta-1,4-galactosyltransferase 2

enzyme: Beta-1,4-galactosyltransferase 2


organism: Rattus norvegicus

substrate_name: N-Acetyl-D-glucosamine; UDP-D-galactose

sequence: PGATLQXAXXLLVAVXAXPPPPLGVXPKPXPG

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

substrate: N-Acetyl-D-glucosamine; UDP-D-galactose


substrate_name: ganglioside GD3

proof_lines: inhibition of GM2 synthesis, pH 7.35, 37°C

substrate: ganglioside GD3


organism: Arabidopsis thaliana

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


organism: Vitis vinifera

substrate_name: dTDP-Glc (in the presence of 100 uM quercitin)


organism: Mus musculus


enzyme_display_name: Anthocyanidin 3-O-glucosyltransferase 1

organism: Fragaria ananassa

substrate_name: pelargonidin

proof_lines: The kinetic constants are determined for the recombinant His(6)-tagged protein.

enzyme: Anthocyanidin 3-O-glucosyltransferase 1

substrate: pelargonidin


substrate_name: UDP-glucose

substrate: UDP-glucose


substrate_name: delphinidin

substrate: delphinidin


substrate_name: cyanidin

substrate: cyanidin


substrate_name: malvidin

substrate: malvidin


substrate_name: UDPglucose

substrate: UDPglucose


substrate_name: Unknown

substrate: Unknown


enzyme_display_name: UDP-glucose flavonoid 3-O-glucosyltransferase 7

proof_lines: The kinetic constants are determined for the recombinant GST-fusion protein.


enzyme_display_name: UDP-glucose flavonoid 3-O-glucosyltransferase 6


proof_lines: inhibition of GD2 synthesis, pH 7.35, 37°C


substrate_name: UDP-galactose

substrate: UDP-galactose


substrate_name: D-GlcNAc

substrate: D-GlcNAc


substrate_name: UDP-N-acetyl-D-galactosamine

proof_lines:
cosubstrate GM3
cosubstrate SM3

substrate: UDP-N-acetyl-D-galactosamine


substrate_name: kaempferol

substrate: kaempferol


substrate_name: UDP-GlcNAc (in the presence of 100 uM quercitin)

proof_lines: VvGT1 UDP-glucose kaempferol


substrate_name: UDP-Man (in the presence of 100 uM quercitin)


substrate_name: GDP-Glc (in the presence of 100 uM quercitin)


substrate_name: UDP-Xyl (in the presence of 100 uM quercitin)


organism: Camellia sinensis

column_label: Table 1; row Kaempferol under enzyme CsUGT78A14, GlcT activity; column Km (uM) raw 30.9+/-3.5; column kcat (S^-1) raw 6.75+/-0.03; column kcat/Km raw 0.219 s^-1 uM^-1.

normalized_value_unit_evidence: Km 30.9 uM = 30.9/1000 = 0.0309 mM. kcat 6.75 s^-1 = 6.75 s^-1 (already canonical). kcat/Km taken by division of this same row's kcat and Km: 6.75 / 0.0309 = 218.45 s^-1 mM^-1; the table's own rounded efficiency 0.219 s^-1 uM^-1 = 0.219 x 1000 = 219 s^-1 mM^-1, consistent with the division at the rounding printed in the table.

raw_value_unit_evidence: Table 1, CsUGT78A14 GlcT activity block, Kaempferol row: Km 30.9+/-3.5 uM; kcat 6.75+/-0.03 s^-1; kcat/Km 0.219 s^-1 uM^-1.

row_label: CsUGT78A14 GlcT activity; Kaempferol

table_label: Table 1. Kinetic parameters of recombinant CsUGT78A14 and CsUGT78A15 proteins.


substrate_name: myricetin

column_label: Table 1; row Myricetin under enzyme CsUGT78A14, GlcT activity; column Km (uM) raw 23.8+/-2.1; column kcat (S^-1) raw 3.11+/-0.04; column kcat/Km raw 0.131 s^-1 uM^-1.

normalized_value_unit_evidence: Km 23.8 uM = 23.8/1000 = 0.0238 mM. kcat 3.11 s^-1 = 3.11 s^-1 (already canonical). kcat/Km taken by division of this same row's kcat and Km: 3.11 / 0.0238 = 130.67 s^-1 mM^-1; the table's own rounded efficiency 0.131 s^-1 uM^-1 = 0.131 x 1000 = 131 s^-1 mM^-1, consistent with the division at the rounding printed in the table.

raw_value_unit_evidence: Table 1, CsUGT78A14 GlcT activity block, Myricetin row: Km 23.8+/-2.1 uM; kcat 3.11+/-0.04 s^-1; kcat/Km 0.131 s^-1 uM^-1.

row_label: CsUGT78A14 GlcT activity; Myricetin

substrate: myricetin


substrate_name: quercetin

column_label: Table 1; row Quercetin under enzyme CsUGT78A14, GlcT activity; column Km (uM) raw 11.9+/-1.6; column kcat (S^-1) raw 3.32+/-0.07; column kcat/Km raw 0.280 s^-1 uM^-1.

normalized_value_unit_evidence: Km 11.9 uM = 11.9/1000 = 0.0119 mM. kcat 3.32 s^-1 = 3.32 s^-1 (already canonical). kcat/Km taken by division of this same row's kcat and Km: 3.32 / 0.0119 = 278.99 s^-1 mM^-1; the table's own rounded efficiency 0.280 s^-1 uM^-1 = 0.280 x 1000 = 280 s^-1 mM^-1, consistent with the division at the rounding printed in the table.

raw_value_unit_evidence: Table 1, CsUGT78A14 GlcT activity block, Quercetin row: Km 11.9+/-1.6 uM; kcat 3.32+/-0.07 s^-1; kcat/Km 0.280 s^-1 uM^-1.

row_label: CsUGT78A14 GlcT activity; Quercetin

substrate: quercetin


column_label: Table 1; row UDP-Glc* under enzyme CsUGT78A14, GlcT activity; column Km (uM) raw 14.1+/-1.8; column kcat (S^-1) raw 3.61+/-0.02; column kcat/Km raw 0.257 s^-1 uM^-1.

normalized_value_unit_evidence: Km 14.1 uM = 14.1/1000 = 0.0141 mM. kcat 3.61 s^-1 = 3.61 s^-1 (already canonical). kcat/Km taken by division of this same row's kcat and Km: 3.61 / 0.0141 = 256.03 s^-1 mM^-1; the table's own rounded efficiency 0.257 s^-1 uM^-1 = 0.257 x 1000 = 257 s^-1 mM^-1, consistent with the division at the rounding printed in the table.

raw_value_unit_evidence: Table 1, CsUGT78A14 GlcT activity block, UDP-Glc* row: Km 14.1+/-1.8 uM; kcat 3.61+/-0.02 s^-1; kcat/Km 0.257 s^-1 uM^-1.

row_label: CsUGT78A14 GlcT activity; UDP-Glc*


substrate_name: curcumin

column_label: Table 3 section Curcumin, row UGT71C1, column Km (mM), gives 0.15 +/- 0.01

row_label: UGT71C1 / Curcumin

table_label: Table 3

substrate: curcumin


substrate_name: trans-resveratrol

column_label: Table 3 section Trans-resveratrol (Trans-resveratrol-3-O-glucoside formation), row UGT71C1, column Km (mM), gives a0.5 +/- 0.1

row_label: UGT71C1 / Trans-resveratrol 3-O-glucoside formation

substrate: trans-resveratrol


substrate_name: vanillin

column_label: Table 3 section Vanillin, row UGT71C1, column Km (mM), gives 1.12 +/- 0.04

row_label: UGT71C1 / Vanillin

substrate: vanillin


substrate_name: etoposide

column_label: Table 3 section Etoposide, row UGT71C1, column Km (mM), gives 2.7 +/- 0.2

row_label: UGT71C1 / Etoposide

substrate: etoposide


organism: Fragaria x ananassa

substrate_name: isorhamnetin

substrate: isorhamnetin


substrate_name: UDP-alpha-D-glucose

proof_lines:
pH 7.0, 30°C, enzyme FaGT7
pH 7.0, 30°C, enzyme FaGT6

substrate: UDP-alpha-D-glucose


substrate_name: ganglioside GM3

substrate: ganglioside GM3


smiles: [Gd]


substrate_name: lactosylceramide

substrate: lactosylceramide


enzyme_display_name: sucrose:sucrose fructosyltransferase

organism: Agave tequilana

substrate_name: sucrose

enzyme: sucrose:sucrose fructosyltransferase

substrate: sucrose


organism: Helianthus tuberosus


organism: Cichorium intybus


substrate_name: fructose

substrate: fructose


organism: Vitis labrusca

substrate_name: cyanidin

column_label: Official ScienceDirect HTML Table 1 row Cyanidin (3), VL3GT columns KM=4.79 +/- 0.69 uM, kcat=7.02 in 10^-4 s^-1 units, kcat/KM=146 M^-1 s^-1; Section 5.7 states acceptor kinetics used 9 mM UDPG with varying cyanidin.

row_label: Cyanidin (3) / VL3GT

table_label: Table 1. Kinetic analysis of VL3GT and VV3GT with cyanidin, quercetin and UDPG.

substrate: cyanidin


column_label: Official ScienceDirect HTML Table 1 row Quercetin (6), VL3GT columns KM=2.16 +/- 0.28 uM, kcat=1.13 in 10^-4 s^-1 units, kcat/KM=52 M^-1 s^-1; Section 5.7 states acceptor kinetics used 9 mM UDPG with varying quercetin.

row_label: Quercetin (6) / VL3GT


column_label: Official ScienceDirect HTML Table 1 row UDPG (cyanidin)/(quercetin), VL3GT columns KM=914 +/- 0.38 uM, kcat=2.47 in 10^-4 s^-1 units, kcat/KM=0.27 M^-1 s^-1; Section 5.7 states UDPG kinetics used 6 uM cyanidin.

row_label: UDPG (cyanidin) / VL3GT


substrate_name: kaempferol

column_label: Publisher PDF page 6, Table 2, Kae (5) row under Km(app), kcat, and kcat/Km(app).

normalized_value_unit_evidence: Km=0.012 mM; kcat=0.5955 s^-1; explicit paper efficiency=49.6 s^-1 mM^-1.

row_label: Kae (5)

table_label: Table 2, Kinetic parameters of recombinant flavonol 3-O-GT


column_label: Publisher PDF page 6, Table 2, Myr (7) row under Km(app), kcat, and kcat/Km(app).

normalized_value_unit_evidence: Km=0.033 mM; kcat=0.6252 s^-1; explicit paper efficiency=18.9 s^-1 mM^-1.

row_label: Myr (7)


column_label: Publisher PDF page 6, Table 2, Que (6) row under Km(app), kcat, and kcat/Km(app).

normalized_value_unit_evidence: Km=0.067 mM; kcat=1.0470 s^-1; explicit paper efficiency=15.6 s^-1 mM^-1.

row_label: Que (6)


substrate_name: UDP-glucose

column_label: Publisher PDF page 6, Table 2, UDPG row and footnote b under Km(app), kcat, and kcat/Km(app).

normalized_value_unit_evidence: Km=0.669 mM; kcat=1.4425 s^-1; explicit paper efficiency=2.2 s^-1 mM^-1.

raw_value_unit_evidence: Km(app)=669 microM (footnote b: quercetin co-substrate); kcat=1.4425 s^-1; kcat/Km(app)=0.0022 microM^-1 s^-1.

row_label: UDPG

substrate: UDP-glucose


enzyme_display_name: Alpha,alpha-trehalose phosphorylase

organism: Geobacillus stearothermophilus

substrate_name: trehalose

column_label: Table 3 lists Apparent Km (mM) for phosphorolysis with Trehalose under the B. stearo(a) column as 4.1; the Kinetics prose also states Km for trehalose and Pi were 4.1 and 1.1 mM respectively.

raw_value_unit_evidence: Km raw_value=4.1 raw_unit=mM

row_label: Apparent Km (mM) / Phosphorolysis / Trehalose / B. stearo(a) column

table_label: Table 3. Properties of Trehalose Phosphorylases from Various Origins

enzyme: Alpha,alpha-trehalose phosphorylase

substrate: trehalose