sequence_source: UniProtKB REST A0A0G2QNK1 FASTA fetched 2026-05-31; mutation_signature L386W/G417L applied after residue check

sequence_variant_note: Mutation signature L386W/G417L matches the mutant residue at offset +0 and the reference residue at offset -35; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


enzyme_display_name: laccase

organism: Bacillus pumilus

source_db: oed

verification_status: unverified

ki_display: Not reported

mutation_signature: Unknown

protein_accession_database: UniProt

kcat_over_km_origin: calculated

source_license: CC BY 4.0

km_unit: mM

sequence_source: UniProtKB REST A0A0G2QNK1 FASTA fetched 2026-05-31

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

data_origin: experimental

enzyme: laccase

status: unverified

source: oed


organism: Bacillus altitudinis

substrate_name: Sinapoylcholine

sequence_source: UniProtKB direct accession lookup:A0A193DSS0; retrieved 2026-05-31

substrate: Sinapoylcholine


substrate_name: sinapate

substrate: sinapate


organism: Bacillus amyloliquefaciens

sequence_source: UniProtKB direct accession lookup:A0A6C0GY11; retrieved 2026-05-31


kcat_over_km_display: Not reported

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution


organism: Bacillus clausii

sequence_source: UniProtKB direct accession lookup:Q5WEM6; retrieved 2026-05-31


organism: Acidobacteriaceae


organism: Acinetobacter baumannii

sequence_source: UniProtKB:D0C997 canonical FASTA, checked 2026-05-31


substrate_name: catechol

substrate: catechol


substrate_name: Guaiacol

substrate: Guaiacol


organism: Aeromonas hydrophila

sequence_source: UniProtKB REST direct accession lookup: D5FL31, retrieved 2026-05-31


organism: Agrobacterium sp. S5-1

sequence_source: UniProtKB REST direct accession lookup: A0A0F6SIA9, retrieved 2026-05-31


organism: alpha proteobacterium Q-1


substrate_name: I-

substrate: I-


organism: Aquifex aeolicus

sequence_source: UniProtKB REST direct accession lookup: O67206, retrieved 2026-05-31


substrate_name: Syringaldazine

substrate: Syringaldazine


substrate_name: Cu+

substrate: Cu+


organism: Bacillus coagulans


organism: Bacillus licheniformis

sequence_source: UniProtKB:Q65MU7 direct REST lookup on 2026-05-31


sequence_source: UniProtKB:H8WGE4 direct REST lookup on 2026-05-31


substrate_name: Reactive black 5

sequence_source: UniProtKB:A0A0G2QNK1 direct accession lookup on 2026-05-31; reconstructed variant sequence for T415D after residue checks.

sequence_variant_note: Mutation signature T415D matches the mutant residue at offset +0 and the reference residue at offset +3; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

substrate: Reactive black 5


sequence_source: UniProtKB:A0A0G2QNK1 direct accession lookup on 2026-05-31; reconstructed variant sequence for T418K/Q442A after residue checks.

sequence_variant_note: Mutation signature T418K/Q442A matches the mutant residue at offset +0 and the reference residue at offset +26; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:A0A0G2QNK1 direct accession lookup on 2026-05-31; canonical wild-type sequence.


sequence_source: UniProtKB:A0A0G2QNK1 direct accession lookup on 2026-05-31; reconstructed variant sequence for T418K after residue checks.

sequence_variant_note: Mutation signature T418K matches the mutant residue at offset +0 and the reference residue at offset -3; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:A0A0G2QNK1 direct accession lookup on 2026-05-31; reconstructed variant sequence for T415D/Q442A after residue checks.

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


organism: Bacillus sp.

sequence_source: UniProtKB:B9W2C5 direct accession lookup on 2026-05-31; canonical wild-type sequence.


organism: Bacillus sp. HR03

sequence_source: UniProtKB B9W2C5 canonical sequence checked directly; reconstructed by applying E188F after confirming residue E at position 188.

sequence_variant_note: Mutation signature E188F matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB B9W2C5 canonical sequence checked directly; reconstructed by applying E188Y after confirming residue E at position 188.

sequence_variant_note: Mutation signature E188Y matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB REST A0A0G2QNK1 FASTA fetched 2026-05-31; mutation_signature S208G applied after residue check


sequence_source: UniProtKB REST A0A0G2QNK1 FASTA fetched 2026-05-31; mutation_signature S208G/F227A applied after residue checks

sequence_variant_note: Mutation signature S208G/F227A matches the mutant residue at offset +0 and the reference residue at offset -29; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB REST A0A0G2QNK1 FASTA fetched 2026-05-31; mutation_signature F227A applied after residue check

sequence_variant_note: Mutation signature F227A matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


substrate_name: Malachite green

substrate: Malachite green


sequence_source: UniProtKB REST A0A0G2QNK1 FASTA fetched 2026-05-31; mutation_signature Q442A applied after residue check

sequence_variant_note: Mutation signature Q442A matches the mutant residue at offset +0 and the reference residue at offset -15; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB REST A0A0G2QNK1 FASTA fetched 2026-05-31; mutation_signature T418K applied after residue check


sequence_source: UniProtKB REST A0A0G2QNK1 FASTA fetched 2026-05-31; mutation_signature T418K/Q442A applied after residue checks


sequence_source: UniProtKB B9W2C5 canonical sequence; E188R reconstructed during curation review

sequence_variant_note: Mutation signature E188R matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB B9W2C5 canonical sequence; E188A reconstructed during curation review

sequence_variant_note: Mutation signature E188A matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB B9W2C5 canonical sequence; E188I reconstructed during curation review

sequence_variant_note: Mutation signature E188I matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB B9W2C5 canonical sequence; E188L reconstructed during curation review

sequence_variant_note: Mutation signature E188L matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB B9W2C5 canonical sequence; E188V reconstructed during curation review

sequence_variant_note: Mutation signature E188V matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB B9W2C5 canonical sequence; E188K reconstructed during curation review

sequence_variant_note: Mutation signature E188K matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


organism: Bacillus sp. (in: Bacteria)


sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


substrate_name: L-Dopa

sequence_source: UniProtKB:B9W2C5 direct REST lookup on 2026-05-31

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

substrate: L-Dopa


organism: Bacillus sp. SL-1

sequence_source: UniProtKB REST direct lookup A0A165W3F6; entry A0A165W3F6_9BACI, Bacillus sp. SL-1 laccase EC 1.10.3.2, 513 aa, reference PMID 27059481.


organism: Bacillus subtilis

sequence_source: UniProtKB REST direct lookup P07788 canonical CotA sequence; residue 502 is M and the selected source mutation M502L was reconstructed by replacing only residue 502 with leucine.


substrate_name: Potassium ferrocyanide

sequence_source: UniProtKB REST direct lookup P07788 canonical CotA sequence; residue 502 is M and the selected source mutation M502F was reconstructed by replacing only residue 502 with phenylalanine.

substrate: Potassium ferrocyanide


sequence_source: UniProtKB:P07788 canonical sequence with validated M502F substitution reconstructed on 2026-05-31


sequence_source: UniProtKB:P07788 reviewed FASTA/JSON direct lookup on 2026-05-31


sequence_source: UniProtKB:P07788 canonical sequence with validated I494A substitution reconstructed on 2026-05-31

sequence_variant_note: Mutation signature I494A matches the mutant residue at offset +0 and the reference residue at offset +14; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:P07788 canonical sequence with validated L386A substitution reconstructed on 2026-05-31


sequence_source: uniprot:P07788 + mutation:I494A

sequence_variant_note: Resolved canonical sequence from uniprot:P07788 + mutation:I494A and applied mutation signature I494A to reconstruct the assayed variant sequence.


substrate_name: Sudan Orange G

substrate: Sudan Orange G


sequence_source: uniprot:P07788 + mutation:G417L

sequence_variant_note: Resolved canonical sequence from uniprot:P07788 + mutation:G417L and applied mutation signature G417L to reconstruct the assayed variant sequence.


sequence_source: uniprot:P07788 + mutation:E498D

sequence_variant_note: Resolved canonical sequence from uniprot:P07788 + mutation:E498D and applied mutation signature E498D to reconstruct the assayed variant sequence.


sequence_source: uniprot:P07788 + mutation:E498L

sequence_variant_note: Resolved canonical sequence from uniprot:P07788 + mutation:E498L and applied mutation signature E498L to reconstruct the assayed variant sequence.


sequence_source: uniprot:P07788 + mutation:E498T

sequence_variant_note: Resolved canonical sequence from uniprot:P07788 + mutation:E498T and applied mutation signature E498T to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:P07788 direct FASTA; reconstructed E498T by replacing canonical residue E at position 498 with T during review on 2026-05-31

sequence_variant_note: Mutation signature E498T matches the mutant residue at offset +0 and the reference residue at offset -2; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:P07788 direct FASTA; reconstructed E498L by replacing canonical residue E at position 498 with L during review on 2026-05-31

sequence_variant_note: Mutation signature E498L matches the mutant residue at offset +0 and the reference residue at offset -2; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:P07788 direct FASTA; reconstructed E498D by replacing canonical residue E at position 498 with D during review on 2026-05-31

sequence_variant_note: Mutation signature E498D matches the mutant residue at offset +0 and the reference residue at offset -2; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:P07788 direct FASTA; reconstructed C322A by replacing canonical residue C at position 322 with A during review on 2026-05-31

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


substrate_name: syringaldehyde

sequence_source: UniProtKB:P07788 direct FASTA canonical sequence checked during review on 2026-05-31

substrate: syringaldehyde


substrate_name: acetosyringone

substrate: acetosyringone


substrate_name: Methyl syringate

substrate: Methyl syringate


sequence_source: UniProtKB:P07788 canonical sequence direct lookup on 2026-05-31.


sequence_source: UniProtKB:P07788 canonical sequence direct lookup on 2026-05-31; T415A reconstructed after validating the reference residue in P07788.


sequence_source: UniProtKB:P07788 canonical sequence direct lookup on 2026-05-31; R416A reconstructed after validating the reference residue in P07788.

sequence_variant_note: Mutation signature R416A matches the mutant residue at offset +0 and the reference residue at offset +13; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB B9W2C5 canonical sequence retrieved by UniProt REST on 2026-05-31; row-specific T415G variant reconstructed after confirming canonical T415/T418 residues.

sequence_variant_note: Mutation signature T415G matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB B9W2C5 canonical sequence retrieved by UniProt REST on 2026-05-31; row-specific T418I variant reconstructed after confirming canonical T415/T418 residues.


sequence_source: UniProtKB B9W2C5 canonical sequence retrieved by UniProt REST on 2026-05-31; row-specific T415G/T418I variant reconstructed after confirming canonical T415/T418 residues.


sequence_source: UniProtKB B9W2C5 canonical sequence retrieved by UniProt REST on 2026-05-31; row-specific T415I variant reconstructed after confirming canonical T415/T418 residues.

sequence_variant_note: Mutation signature T415I matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: Direct UniProt REST check of P07788 on 2026-05-31; canonical sequence was 513 aa and R146K was applied after residue R146 matched.


sequence_source: Direct UniProt REST check of P07788 on 2026-05-31; canonical sequence was 513 aa and P455S was applied after residue P455 matched.


sequence_source: Direct UniProt REST check of P07788 on 2026-05-31; canonical sequence was 513 aa and V403T plus P455S were applied after residues V403 and P455 matched.


sequence_source: Direct UniProt REST check of P07788 on 2026-05-31; canonical sequence was 513 aa and F207Y was applied after residue F207 matched.


sequence_source: Direct UniProt REST check of P07788 on 2026-05-31; canonical sequence was 513 aa and V403T was applied after residue V403 matched.

sequence_variant_note: Mutation signature V403T matches the mutant residue at offset +0 and the reference residue at offset -6; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: Direct UniProt REST check of A0A386JCD3 on 2026-05-31; canonical sequence was 513 aa and R155W, M157H, and A158E were applied after residues R155, M157, and A158 matched.


sequence_source: Direct UniProt REST check of A0A386JCD3 on 2026-05-31; canonical sequence was 513 aa and R155H plus A158E were applied after residues R155 and A158 matched.


enzyme_display_name: Laccase

verification_status: verified

column_label: Table 3: Kinetic parameters of CAR2, CAHH1, and mutant forms (Applied Microbiology and Biotechnology 2018, volume 102, pages 4039-4048); printed column heads Group | Clone | ABTS block Km (mM), kcat (s^-1), kcat/Km (s^-1 mM^-1) | DMP block Km (mM), Kcat (s^-1), kcat/Km (s^-1 mM^-1). Table 3, ABTS block, clone row CAR2 wild type; column Km (mM) = 0.21; column kcat (s^-1) = 8; column kcat/Km (s^-1 mM^-1) = 38. captured primary segment: CAR2 wild type | ABTS | Km 0.21 ± 0.02 mM | kcat 8 ± 0.03 s^-1 | kcat/Km 38 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 3 Km cell 0.21 mM; the printed unit is already the canonical millimolar unit, so no unit conversion applies and the value is 0.21 mM. kcat: printed Table 3 kcat cell 8 s^-1; the printed unit is already the canonical per-second unit, so no unit conversion applies and the value is 8.0 s^(-1). kcat/Km: printed Table 3 kcat/Km cell 38 s^-1 mM^-1; the printed unit is already the canonical per-second per-millimolar unit, so no unit conversion applies and the value is 38.0 s^(-1)*mM^(-1). Quotient disclosure for this cell group: 8 / 0.21 = 38.095238095238095 s^-1 mM^-1, which is the exported value, and the printed efficiency cell 38 is that quotient written to the integer place the table uses.

raw_value_unit_evidence: Table 3, ABTS block, clone row CAR2 wild type: Km = 0.21 mM; kcat = 8 s^-1; printed kcat/Km = 38 s^-1 mM^-1.

enzyme: Laccase

status: verified


verification_status: corrected

sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:A0A386JCD3 and applied mutation signature R155H to reconstruct the assayed variant sequence.

column_label: Table 3: Kinetic parameters of CAR2, CAHH1, and mutant forms (Applied Microbiology and Biotechnology 2018, volume 102, pages 4039-4048); printed column heads Group | Clone | ABTS block Km (mM), kcat (s^-1), kcat/Km (s^-1 mM^-1) | DMP block Km (mM), Kcat (s^-1), kcat/Km (s^-1 mM^-1). Table 3, DMP block, clone row CAH2 R155H; column Km (mM) = 0.08; column kcat (s^-1) = 62; column kcat/Km (s^-1 mM^-1) = 786. captured primary segment: CAH2 R155H | DMP | Km 0.08 ± 0.009 mM | kcat 62 ± 0.8 s^-1 | kcat/Km 786 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 3 Km cell 0.08 mM; the printed unit is already the canonical millimolar unit, so no unit conversion applies and the value is 0.08 mM. kcat: printed Table 3 kcat cell 62 s^-1; the printed unit is already the canonical per-second unit, so no unit conversion applies and the value is 62.0 s^(-1). kcat/Km: printed Table 3 kcat/Km cell 786 s^-1 mM^-1; the printed unit is already the canonical per-second per-millimolar unit, so no unit conversion applies and the value is 786.0 s^(-1)*mM^(-1). Quotient disclosure for this cell group: 62 / 0.08 = 775.0 s^-1 mM^-1, against the printed efficiency cell 786.0 s^-1 mM^-1, so the printed cell is the paper's own fitted efficiency and not the quotient of its two rounded component cells.

raw_value_unit_evidence: Table 3, DMP block, clone row CAH2 R155H: Km = 0.08 mM; kcat = 62 s^-1; printed kcat/Km = 786 s^-1 mM^-1.

status: corrected


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature T158A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature R155H to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature R155W/M157H/A158E to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature R155W/M157H to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:A0A386JCD3 and applied mutation signature R155W to reconstruct the assayed variant sequence.

column_label: Table 3: Kinetic parameters of CAR2, CAHH1, and mutant forms (Applied Microbiology and Biotechnology 2018, volume 102, pages 4039-4048); printed column heads Group | Clone | ABTS block Km (mM), kcat (s^-1), kcat/Km (s^-1 mM^-1) | DMP block Km (mM), Kcat (s^-1), kcat/Km (s^-1 mM^-1). Table 3, DMP block, clone row CAW R155W; column Km (mM) = 0.11; column kcat (s^-1) = 19; column kcat/Km (s^-1 mM^-1) = 172. captured primary segment: CAW R155W | DMP | Km 0.11 ± 0.03 mM | kcat 19 ± 0.3 s^-1 | kcat/Km 172 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 3 Km cell 0.11 mM; the printed unit is already the canonical millimolar unit, so no unit conversion applies and the value is 0.11 mM. kcat: printed Table 3 kcat cell 19 s^-1; the printed unit is already the canonical per-second unit, so no unit conversion applies and the value is 19.0 s^(-1). kcat/Km: printed Table 3 kcat/Km cell 172 s^-1 mM^-1; the printed unit is already the canonical per-second per-millimolar unit, so no unit conversion applies and the value is 172.0 s^(-1)*mM^(-1). Quotient disclosure for this cell group: 19 / 0.11 = 172.72727272727272 s^-1 mM^-1, against the printed efficiency cell 172.0 s^-1 mM^-1, so the printed cell is the paper's own fitted efficiency and not the quotient of its two rounded component cells.

raw_value_unit_evidence: Table 3, DMP block, clone row CAW R155W: Km = 0.11 mM; kcat = 19 s^-1; printed kcat/Km = 172 s^-1 mM^-1.

row_label: Table 3, DMP block, clone row CAW R155W


sequence_source: UniProtKB:A0A386JCD3 canonical sequenceVersion=1; row-specific variant reconstructed from R155H/A158E


sequence_source: UniProtKB:A0A1W6S310 canonical sequenceVersion=1 from direct UniProt REST lookup

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

column_label: Table 3: Kinetic parameters of CAR2, CAHH1, and mutant forms (Applied Microbiology and Biotechnology 2018, volume 102, pages 4039-4048); printed column heads Group | Clone | ABTS block Km (mM), kcat (s^-1), kcat/Km (s^-1 mM^-1) | DMP block Km (mM), Kcat (s^-1), kcat/Km (s^-1 mM^-1). Table 3, DMP block, clone row CAHH1 wild type; column Km (mM) = 0.07; column kcat (s^-1) = 79; column kcat/Km (s^-1 mM^-1) = 1134. captured primary segment: CAHH1 wild type | DMP | Km 0.07 ± 0.008 mM | kcat 79 ± 0.9 s^-1 | kcat/Km 1134 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 3 Km cell 0.07 mM; the printed unit is already the canonical millimolar unit, so no unit conversion applies and the value is 0.07 mM. kcat: printed Table 3 kcat cell 79 s^-1; the printed unit is already the canonical per-second unit, so no unit conversion applies and the value is 79.0 s^(-1). kcat/Km: printed Table 3 kcat/Km cell 1134 s^-1 mM^-1; the printed unit is already the canonical per-second per-millimolar unit, so no unit conversion applies and the value is 1134.0 s^(-1)*mM^(-1). Quotient disclosure for this cell group: 79 / 0.07 = 1128.5714285714284 s^-1 mM^-1, against the printed efficiency cell 1134.0 s^-1 mM^-1, so the printed cell is the paper's own fitted efficiency and not the quotient of its two rounded component cells.

raw_value_unit_evidence: Table 3, DMP block, clone row CAHH1 wild type: Km = 0.07 mM; kcat = 79 s^-1; printed kcat/Km = 1134 s^-1 mM^-1.


sequence_source: UniProtKB:A0A386JCD3 canonical sequenceVersion=1 from direct UniProt REST lookup

column_label: Table 3: Kinetic parameters of CAR2, CAHH1, and mutant forms (Applied Microbiology and Biotechnology 2018, volume 102, pages 4039-4048); printed column heads Group | Clone | ABTS block Km (mM), kcat (s^-1), kcat/Km (s^-1 mM^-1) | DMP block Km (mM), Kcat (s^-1), kcat/Km (s^-1 mM^-1). Table 3, DMP block, clone row CAR2 wild type; column Km (mM) = 0.14; column kcat (s^-1) = 21; column kcat/Km (s^-1 mM^-1) = 150. captured primary segment: CAR2 wild type | DMP | Km 0.14 ± 0.01 mM | kcat 21 ± 0.4 s^-1 | kcat/Km 150 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 3 Km cell 0.14 mM; the printed unit is already the canonical millimolar unit, so no unit conversion applies and the value is 0.14 mM. kcat: printed Table 3 kcat cell 21 s^-1; the printed unit is already the canonical per-second unit, so no unit conversion applies and the value is 21.0 s^(-1). kcat/Km: printed Table 3 kcat/Km cell 150 s^-1 mM^-1; the printed unit is already the canonical per-second per-millimolar unit, so no unit conversion applies and the value is 150.0 s^(-1)*mM^(-1). Quotient disclosure for this cell group: 21 / 0.14 = 149.99999999999997 s^-1 mM^-1, which is the printed efficiency cell itself.

raw_value_unit_evidence: Table 3, DMP block, clone row CAR2 wild type: Km = 0.14 mM; kcat = 21 s^-1; printed kcat/Km = 150 s^-1 mM^-1.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:A0A386JCD3 and applied mutation signature R155W/M157H to reconstruct the assayed variant sequence.

column_label: Table 3: Kinetic parameters of CAR2, CAHH1, and mutant forms (Applied Microbiology and Biotechnology 2018, volume 102, pages 4039-4048); printed column heads Group | Clone | ABTS block Km (mM), kcat (s^-1), kcat/Km (s^-1 mM^-1) | DMP block Km (mM), Kcat (s^-1), kcat/Km (s^-1 mM^-1). Table 3, DMP block, clone row CAWH R155W/M157H; column Km (mM) = 0.09; column kcat (s^-1) = 49; column kcat/Km (s^-1 mM^-1) = 545. captured primary segment: CAWH R155W/M157H | DMP | Km 0.09 ± 0.008 mM | kcat 49 ± 0.6 s^-1 | kcat/Km 545 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 3 Km cell 0.09 mM; the printed unit is already the canonical millimolar unit, so no unit conversion applies and the value is 0.09 mM. kcat: printed Table 3 kcat cell 49 s^-1; the printed unit is already the canonical per-second unit, so no unit conversion applies and the value is 49.0 s^(-1). kcat/Km: printed Table 3 kcat/Km cell 545 s^-1 mM^-1; the printed unit is already the canonical per-second per-millimolar unit, so no unit conversion applies and the value is 545.0 s^(-1)*mM^(-1). Quotient disclosure for this cell group: 49 / 0.09 = 544.4444444444445 s^-1 mM^-1, against the printed efficiency cell 545.0 s^-1 mM^-1, so the printed cell is the paper's own fitted efficiency and not the quotient of its two rounded component cells.

raw_value_unit_evidence: Table 3, DMP block, clone row CAWH R155W/M157H: Km = 0.09 mM; kcat = 49 s^-1; printed kcat/Km = 545 s^-1 mM^-1.


sequence_source: UniProtKB:A0A386JCD3 canonical sequenceVersion=1; row-specific variant reconstructed from R155W


sequence_source: UniProtKB:A0A348FKE9 canonical sequenceVersion=1 from direct UniProt REST lookup


sequence_variant_note: Resolved canonical sequence from UniProtKB REST B9W2C5; direct accession lookup; reconstructed_variant_sequence and applied mutation signature E188I to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB REST B9W2C5; direct accession lookup; reconstructed_variant_sequence and applied mutation signature E188R to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB REST B9W2C5; direct accession lookup; reconstructed_variant_sequence and applied mutation signature E188K to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB REST B9W2C5; direct accession lookup; reconstructed_variant_sequence and applied mutation signature E188L to reconstruct the assayed variant sequence.


sequence_source: UniProtKB REST B9W2C5; direct accession lookup; canonical_wild_type_sequence


organism: Bacillus sp. LS03

sequence_source: UniProtKB REST H8WGE3; direct accession lookup; canonical_wild_type_sequence


sequence_variant_note: Resolved canonical sequence from UniProtKB REST H8WGE3; direct accession lookup; reconstructed_variant_sequence at offset 0 and applied mutation signature T232P/Q367R to reconstruct the assayed variant sequence.


sequence_source: UniProtKB REST A0A165W3F6; direct accession lookup; canonical_wild_type_sequence


sequence_source: UniProtKB:P07788 canonical sequence; reconstructed R429K variant after residue check on 2026-05-31

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


sequence_source: UniProtKB:P07788 canonical sequence; reconstructed A478F variant after residue check on 2026-05-31


sequence_source: UniProtKB:P07788 canonical sequence; reconstructed T480A variant after residue check on 2026-05-31

sequence_variant_note: Mutation signature T480A matches the mutant residue at offset +0 and the reference residue at offset -14; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:P07788 canonical sequence; reconstructed T480F variant after residue check on 2026-05-31

sequence_variant_note: Mutation signature T480F matches the mutant residue at offset +0 and the reference residue at offset -14; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:P07788 canonical sequence; reconstructed R476K variant after residue check on 2026-05-31

sequence_variant_note: Mutation signature R476K matches the mutant residue at offset +0 and the reference residue at offset +11; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


organism: Escherichia coli

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


sequence_source: UniProtKB:P36649 reviewed FASTA checked 2026-05-31


sequence_source: UniProtKB:P36649 reviewed FASTA checked 2026-05-31; canonical sequence only, not a validated variant sequence

sequence_variant_note: Mutation signature G276K could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature G276N could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset +4, so it is treated as a source-provided variant sequence.


sequence_variant_note: Mutation signature G276Y could not be applied safely because the resolved sequence does not match the expected reference residue(s).


organism: Campylobacter jejuni

smiles: O=O


organism: Coprinopsis cinerea


substrate_name: guaiacol

substrate: guaiacol


organism: Coprinus comatus


substrate_name: syringaldazine

substrate: syringaldazine


organism: Coriolopsis trogii

sequence_source: UniProtKB:A0A140CWW4 canonical sequence, REST lookup on 2026-05-31


organism: Corynebacterium glutamicum

sequence_source: UniProtKB:Q8NLH5 canonical sequence, REST lookup on 2026-05-31


organism: Desulfovibrio sp. A2


organism: Fomitopsis pinicola


organism: Galerina sp. DSM 22662

sequence: GPSTDLVIGNKGFDGGIDSAILR

wild_type_sequence: GPSTDLVIGNKGFDGGIDSAILR

sequence_source: UniProtKB:P86351 fragment entry


organism: Ganoderma fornicatum


sequence_source: UniProtKB:B5G555 REST record


organism: Geobacillus sp. JS12


organism: Geobacillus sp. WSUCF1


organism: Halobacterium volcanii

sequence_source: UniProtKB:D4GPK6 REST record

column_label: Applied and Environmental Microbiology 76(3):733-743 (2010); TABLE 3. Kinetic parameters of H. volcanii LccA compared to those of selected laccase-like enzymes; LccA, H. volcanii block; sub-row 'Culture broth of H. volcanii SB01'; ABTS oxidation column group; column 'Km (uM)' = 700; column 'kcat (s^-1)' = 10.0; column 'kcat/Km (s^-1 uM^-1)' = 0.014.

normalized_value_unit_evidence: Printed kcat 10.0 s^-1 is already the catalog unit s^(-1); no conversion needed; catalog kcat = 10.0. Printed Km 700 uM / 1000 = 0.7 mM, matching the catalog km 0.7 mM exactly. The paper prints a kcat/Km cell for this table row, 0.014 s^-1 uM^-1 = 14.0 s^(-1)*mM^(-1), but that printed cell is NOT the catalog value: the catalog kcat_over_km 14.285714285714286 s^(-1)*mM^(-1) is the exact full-precision quotient of this row's own printed pair, 10.0 / 0.7. The printed cell is rounded to two significant figures, so the ratio is typed derived_same_measurement and the printed cell is documented in prose only.

raw_value_unit_evidence: TABLE 3, LccA H. volcanii block, sub-row 'Culture broth of H. volcanii SB01', ABTS oxidation column group: printed Km (uM) 700; printed kcat (s^-1) 10.0; printed kcat/Km (s^-1 uM^-1) 0.014. In the stored markup the Km column header carries U+03BC GREEK SMALL LETTER MU and every exponent minus is U+2212.

row_label: TABLE 3, LccA H. volcanii block, sub-row 'Culture broth of H. volcanii SB01', ABTS oxidation column group; printed Km (uM) 700, printed kcat (s^-1) 10.0, printed kcat/Km (s^-1 uM^-1) 0.014


organism: Klebsiella sp. 601

sequence_variant_note: Resolved canonical sequence from UniProtKB B0FMT2 current REST accession check on 2026-05-31; E106F reconstructed after confirming canonical residue 106 is E. and applied mutation signature E106F to reconstruct the assayed variant sequence.


sequence_source: UniProtKB B0FMT2 current REST accession check on 2026-05-31.

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


sequence_source: UniProtKB B0FMT2 current REST accession check on 2026-05-31; E106F reconstructed after confirming canonical residue 106 is E.


organism: Marinomonas mediterranea

sequence_source: UniProtKB Q9KJB8 current REST accession check on 2026-05-31.


organism: Meiothermus ruber

sequence_source: UniProtKB D3PLX1 current REST accession check on 2026-05-31.


sequence_source: UniProtKB:D3PLX1 direct REST lookup on 2026-05-31


organism: Mycothermus thermophilus


organism: Ochrobactrum sp. 531


organism: Paenibacillus glucanolyticus

sequence_source: UniProtKB:A0A165SC98 direct REST lookup on 2026-05-31


organism: Bacillus subtilis


organism: Caldalkalibacillus thermarum

sequence_source: UniProtKB:F5L3U7 direct accession lookup


organism: Campylobacter jejuni

sequence_source: UniProtKB:A0A0H3PBA4 direct accession lookup


organism: Pseudomonas putida

sequence_source: UniProtKB REST FASTA Q88C03; canonical wild-type sequence checked 2026-05-31

assay_conditions_summary: pH 4; 293.15 K; buffer to the 2

column_label: Table 3 (Steady-state kinetic constants for Pp-CopA and Pf-CopA), substrate block 2,6-Dimethoxyphenol (DMP), enzyme row Pp-CopA; column KM [uM] = 1459; column Vmax [U/mg prot.] = 3.4; column kcat [s-1] = 3.97; column Specificity constant kcat/KM [M^-1 s^-1] = 2700, printed with a standard deviation of 100.

normalized_value_unit_evidence: kcat: printed Table 3 cell 3.97 under the head kcat [s-1]; the printed unit is already canonical and no unit conversion applies, so kcat = 3.97 s^(-1). Km: printed Table 3 cell 1459 under the head KM [uM]; 1 uM is 0.001 mM, so 1459 / 1000 = 1.459, and Km = 1.459 mM. kcat/Km: printed Table 3 cell 2700 under the head kcat/KM [M^-1 s^-1]; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 2700 / 1000 = 2.7, and kcat/Km = 2.7 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 3, 2,6-Dimethoxyphenol (DMP) block, Pp-CopA row: KM [uM] cell = 1459; Vmax [U/mg prot.] cell = 3.4; kcat [s-1] cell = 3.97; specificity constant kcat/KM [M^-1 s^-1] cell = 2700, printed with a standard deviation of 100.

table_label: Table 3: Steady-state kinetic constants for Pp-CopA and Pf-CopA (Archives of Biochemistry and Biophysics 2018, author accepted manuscript)


assay_conditions_summary: pH 4; 293.15 K; buffer to the ABTS block

column_label: Table 3 (Steady-state kinetic constants for Pp-CopA and Pf-CopA), substrate block ABTS, enzyme row Pp-CopA; column KM [uM] = 488; column Vmax [U/mg prot.] = 2.0; column kcat [s-1] = 2.40; column Specificity constant kcat/KM [M^-1 s^-1] = 4900, printed with a standard deviation of 90.

normalized_value_unit_evidence: kcat: printed Table 3 cell 2.40 under the head kcat [s-1]; the printed unit is already canonical and no unit conversion applies, so kcat = 2.4 s^(-1). Km: printed Table 3 cell 488 under the head KM [uM]; 1 uM is 0.001 mM, so 488 / 1000 = 0.488, and Km = 0.488 mM. kcat/Km: printed Table 3 cell 4900 under the head kcat/KM [M^-1 s^-1]; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 4900 / 1000 = 4.9, and kcat/Km = 4.9 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 3, ABTS block, Pp-CopA row: KM [uM] cell = 488; Vmax [U/mg prot.] cell = 2.0; kcat [s-1] cell = 2.40; specificity constant kcat/KM [M^-1 s^-1] cell = 4900, printed with a standard deviation of 90.

row_label: Table 3, ABTS block, Pp-CopA row


organism: Pseudomonas sp.

sequence_source: UniProtKB REST FASTA A0A0K1P839; canonical wild-type sequence checked 2026-05-31


organism: Pyrobaculum aerophilum

sequence_source: UniProtKB REST FASTA Q8ZWA8; canonical wild-type sequence checked 2026-05-31


sequence_source: UniProtKB REST FASTA Q8ZWA8; M470F reconstructed after confirming residue 470 was M on 2026-05-31

sequence_variant_note: Mutation signature M470F matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB REST FASTA Q8ZWA8; M470L reconstructed after confirming residue 470 was M on 2026-05-31

sequence_variant_note: Mutation signature M470L matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB live accession A0A165SC98, checked by REST/FASTA on 2026-05-31.

proof_lines: OUP PDF Table 1: ABTS row at pH 7.0 and 25 deg C reports Km 38.8 +/- 11.1 micromol l^-1, Kcat 1400 s^-1, and Kcat/Km 36.1.


proof_lines: OUP PDF Table 1: catechol row at pH 7.0 and 25 deg C reports Km 11669 +/- 2015 micromol l^-1, Kcat 8805 s^-1, and Kcat/Km 0.75.


organism: Pleurotus eryngii

sequence_variant_note: Resolved canonical sequence from UniProtKB live accession B0JDP9 canonical sequence with in-review K532E reconstruction; residue 532 was checked as K on 2026-05-31. and applied mutation signature K532E to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB live accession B0JDP9 canonical sequence with in-review P530A reconstruction; residue 530 was checked as P on 2026-05-31. and applied mutation signature P530A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB live accession B0JDP9 canonical sequence with in-review K532A reconstruction; residue 532 was checked as K on 2026-05-31. and applied mutation signature K532A to reconstruct the assayed variant sequence.


organism: Pleurotus ostreatus

sequence_source: UniProtKB live accession O60199, checked by REST/FASTA on 2026-05-31.


sequence_source: UniProtKB reviewed accession Q12739, checked by REST/FASTA on 2026-05-31.


sequence_source: UniProtKB:D4GPK6 direct lookup on 2026-05-31


organism: Haloferax volcanii

substrate_name: bilirubin

column_label: Applied and Environmental Microbiology 76(3):733-743 (2010); Results, bilirubin specificity sentence; substrate bilirubin; printed 'kcat, 29 s^-1'; printed 'Km, 236 uM'; printed 'kcat/Km value of 0.12 s^-1 uM^-1'.

normalized_value_unit_evidence: Printed kcat 29 s^-1 is already the catalog unit s^(-1); no conversion needed; catalog kcat = 29.0. Printed Km 236 uM / 1000 = 0.236 mM, matching the catalog km 0.236 mM exactly. The paper prints a ratio for this measurement, kcat/Km value of 0.12 s^-1 uM^-1 = 120.0 s^(-1)*mM^(-1), but that printed value is NOT the catalog value: the catalog kcat_over_km 122.88135593220339 s^(-1)*mM^(-1) is the exact full-precision quotient of this measurement's own printed pair, 29.0 / 0.236. The printed ratio is rounded to two significant figures, so the ratio is typed derived_same_measurement and the printed value is documented in prose only.

raw_value_unit_evidence: Results, bilirubin specificity sentence: printed kcat, 29 s^-1; printed Km, 236 uM; printed kcat/Km value of 0.12 s^-1 uM^-1. In the stored asset the micro sign is U+03BC and every exponent minus is U+2212.

row_label: Results bilirubin specificity sentence; printed kcat, 29 s^-1, printed Km, 236 uM, printed kcat/Km value of 0.12 s^-1 uM^-1

table_label: Results, bilirubin specificity sentence (no table; the paper prints this pair in prose)

substrate: bilirubin


column_label: Applied and Environmental Microbiology 76(3):733-743 (2010); Abstract, apparent kinetic parameters sentence; ABTS position; printed Km 670 uM; printed kcat 10 s^-1. The same measurement is printed one digit longer in TABLE 3. Kinetic parameters of H. volcanii LccA compared to those of selected laccase-like enzymes, sub-row 'Culture broth of H. volcanii US02', ABTS oxidation column group: Km (uM) 671, kcat (s^-1) 9.9, kcat/Km (s^-1 uM^-1) 0.015.

normalized_value_unit_evidence: Printed kcat 10 s^-1 is already the catalog unit s^(-1); no conversion needed; catalog kcat = 10.0. Printed Km 670 uM / 1000 = 0.67 mM, matching the catalog km 0.67 mM exactly. The Abstract sentence prints no ratio for this pair. The TABLE 3 sub-row that prints the same US02 ABTS measurement to one more digit (Km 671 uM, kcat 9.9 s^-1) carries the ratio cell 0.015 s^-1 uM^-1 = 15.0 s^(-1)*mM^(-1), which is not the catalog value either: the catalog kcat_over_km 14.925373134328357 s^(-1)*mM^(-1) is the exact full-precision quotient of the Abstract pair, 10.0 / 0.67. The ratio is therefore typed derived_same_measurement and the printed table cell is documented in prose only.

raw_value_unit_evidence: Abstract, apparent kinetic parameters sentence: printed Km 670 uM and printed kcat 10 s^-1 for ABTS. In the stored asset the micro sign is U+03BC and the exponent minus is U+2212.

row_label: Abstract apparent kinetic parameters sentence, ABTS position; printed Km 670 uM and printed kcat 10 s^-1

table_label: Abstract, apparent kinetic parameters sentence (no table; the paper prints this pair in prose)


organism: Klebsiella pneumoniae


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


organism: Rheinheimera sp.


substrate_name: sinapate

substrate: sinapate


organism: Rhizobium meliloti

sequence_source: UniProtKB:A0A0D4CYE3 direct REST lookup on 2026-05-31


sequence_source: UniProtKB REST direct lookup for O60199 on 2026-05-31


sequence_source: UniProtKB REST direct lookup for Q12739 on 2026-05-31


organism: Pseudomonas fluorescens

sequence_source: UniProtKB REST direct lookup for Q4KCN4 on 2026-05-31

column_label: Table 3 (Steady-state kinetic constants for Pp-CopA and Pf-CopA), substrate block 2,6-Dimethoxyphenol (DMP), enzyme row Pf-CopA; column KM [uM] = 822; column Vmax [U/mg prot.] = 1.4; column kcat [s-1] = 1.42; column Specificity constant kcat/KM [M^-1 s^-1] = 1700, printed with a standard deviation of 20.

normalized_value_unit_evidence: kcat: printed Table 3 cell 1.42 under the head kcat [s-1]; the printed unit is already canonical and no unit conversion applies, so kcat = 1.42 s^(-1). Km: printed Table 3 cell 822 under the head KM [uM]; 1 uM is 0.001 mM, so 822 / 1000 = 0.822, and Km = 0.822 mM. kcat/Km: printed Table 3 cell 1700 under the head kcat/KM [M^-1 s^-1]; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1700 / 1000 = 1.7, and kcat/Km = 1.7 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 3, 2,6-Dimethoxyphenol (DMP) block, Pf-CopA row: KM [uM] cell = 822; Vmax [U/mg prot.] cell = 1.4; kcat [s-1] cell = 1.42; specificity constant kcat/KM [M^-1 s^-1] cell = 1700, printed with a standard deviation of 20.


column_label: Table 3 (Steady-state kinetic constants for Pp-CopA and Pf-CopA), substrate block ABTS, enzyme row Pf-CopA; column KM [uM] = 214; column Vmax [U/mg prot.] = 2.2; column kcat [s-1] = 2.20; column Specificity constant kcat/KM [M^-1 s^-1] = 10300, printed with a standard deviation of 160.

normalized_value_unit_evidence: kcat: printed Table 3 cell 2.20 under the head kcat [s-1]; the printed unit is already canonical and no unit conversion applies, so kcat = 2.2 s^(-1). Km: printed Table 3 cell 214 under the head KM [uM]; 1 uM is 0.001 mM, so 214 / 1000 = 0.214, and Km = 0.214 mM. kcat/Km: printed Table 3 cell 10300 under the head kcat/KM [M^-1 s^-1]; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 10300 / 1000 = 10.3, and kcat/Km = 10.3 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 3, ABTS block, Pf-CopA row: KM [uM] cell = 214; Vmax [U/mg prot.] cell = 2.2; kcat [s-1] cell = 2.20; specificity constant kcat/KM [M^-1 s^-1] cell = 10300, printed with a standard deviation of 160.

row_label: Table 3, ABTS block, Pf-CopA row


assay_conditions_summary: pH 7; 293.15 K; buffer pH 7

column_label: Table 3 (Steady-state kinetic constants for Pp-CopA and Pf-CopA), substrate block Syringaldazine (SGZ), enzyme row Pf-CopA; column KM [uM] = 51; column Vmax [U/mg prot.] = 1.2; column kcat [s-1] = 1.22; column Specificity constant kcat/KM [M^-1 s^-1] = 23800, printed with a standard deviation of 500.

normalized_value_unit_evidence: kcat: printed Table 3 cell 1.22 under the head kcat [s-1]; the printed unit is already canonical and no unit conversion applies, so kcat = 1.22 s^(-1). Km: printed Table 3 cell 51 under the head KM [uM]; 1 uM is 0.001 mM, so 51 / 1000 = 0.051, and Km = 0.051 mM. kcat/Km: printed Table 3 cell 23800 under the head kcat/KM [M^-1 s^-1]; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 23800 / 1000 = 23.8, and kcat/Km = 23.8 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 3, Syringaldazine (SGZ) block, Pf-CopA row: KM [uM] cell = 51; Vmax [U/mg prot.] cell = 1.2; kcat [s-1] cell = 1.22; specificity constant kcat/KM [M^-1 s^-1] cell = 23800, printed with a standard deviation of 500.


sequence_source: UniProtKB REST direct lookup for Q88C03 on 2026-05-31

column_label: Table 3 (Steady-state kinetic constants for Pp-CopA and Pf-CopA), substrate block Syringaldazine (SGZ), enzyme row Pp-CopA; column KM [uM] = 26; column Vmax [U/mg prot.] = 1.1; column kcat [s-1] = 1.28; column Specificity constant kcat/KM [M^-1 s^-1] = 49000, printed with a standard deviation of 2000.

normalized_value_unit_evidence: kcat: printed Table 3 cell 1.28 under the head kcat [s-1]; the printed unit is already canonical and no unit conversion applies, so kcat = 1.28 s^(-1). Km: printed Table 3 cell 26 under the head KM [uM]; 1 uM is 0.001 mM, so 26 / 1000 = 0.026, and Km = 0.026 mM. kcat/Km: printed Table 3 cell 49000 under the head kcat/KM [M^-1 s^-1]; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 49000 / 1000 = 49.0, and kcat/Km = 49.0 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 3, Syringaldazine (SGZ) block, Pp-CopA row: KM [uM] cell = 26; Vmax [U/mg prot.] cell = 1.1; kcat [s-1] cell = 1.28; specificity constant kcat/KM [M^-1 s^-1] cell = 49000, printed with a standard deviation of 2000.


sequence_source: UniProtKB exact accession A0A0D4CYE3 REST lookup; live unreviewed entry, organism Rhizobium meliloti, EC 1.10.3.2.


organism: Rhodococcus erythropolis


organism: Staphylococcus haemolyticus

sequence_source: UniProtKB exact accession L7SV51 REST lookup; live unreviewed entry, organism Staphylococcus haemolyticus, multicopper oxidase with no explicit UniProt EC.


organism: Stenotrophomonas maltophilia

sequence_source: UniProtKB exact accession G8XUN7 REST lookup; live unreviewed entry, organism Stenotrophomonas maltophilia, EC 1.10.3.2.


organism: Streptomyces californicus


organism: Streptomyces coelicolor


sequence_source: UniProt:Q9XAL8 live accession check on 2026-05-31; unreviewed TrEMBL copper oxidase from Streptomyces coelicolor strain ATCC BAA-471 / A3(2) / M145.


substrate_name: Morin

sequence_source: UniProtKB REST/FASTA Q9XAL8 canonical sequence, row-specific S292A substitution reconstructed after confirming canonical residue S at position 292.

sequence_variant_note: Mutation signature S292A matches the mutant residue at offset +0 and the reference residue at offset +2; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

substrate: Morin


sequence_source: UniProtKB REST/FASTA Q9XAL8 canonical sequence.


sequence_source: UniProtKB REST/FASTA Q9XAL8 canonical sequence, row-specific V290A substitution reconstructed after confirming canonical residue V at position 290.

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


sequence_source: UniProtKB REST/FASTA Q9XAL8 canonical sequence, row-specific M298F substitution reconstructed after confirming canonical residue M at position 298.


sequence_source: UniProtKB REST/FASTA Q9XAL8 canonical sequence, row-specific V290N substitution reconstructed after confirming canonical residue V at position 290.


sequence_source: UniProtKB REST accession Q9XAL8 checked 2026-05-31


organism: Streptomyces cyaneus

sequence_source: UniProtKB REST accession F6L7B5 checked 2026-05-31


substrate_name: Guaiacol

substrate: Guaiacol


organism: Streptomyces griseoflavus

sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:A0A0M4FJ81 and applied mutation signature H165F to reconstruct the assayed variant sequence.

column_label: Table 3 (Kinetic parameters of the SgfSL variants), ABTS block, protein row 'H165F'; column 'Km (mM)' = 0.45 ± 0.03; column 'kcat (s-1)' = 0.12 ± 0.009; column 'kcat/Km (mM-1 s-1)' = 0.26; column 'Activity nM O2 min-1 ug-1' = 0.13 ± 0.06.

normalized_value_unit_evidence: kcat: printed Table 3 cell 0.12 under the column head kcat (s-1); the cell is already in s^-1, so no unit conversion applies and the canonical value is 0.12 s^(-1). Km: printed Table 3 cell 0.45 under the column head Km (mM); the cell is already in mM, so no unit conversion applies and the canonical value is 0.45 mM. kcat/Km: printed Table 3 cell 0.26 under the column head kcat/Km (mM-1 s-1); the cell is already in mM^-1 s^-1, so no unit conversion applies and the canonical value is 0.26 s^-1 mM^-1.

raw_value_unit_evidence: Table 3, ABTS block, protein row 'H165F': Km = 0.45 mM; kcat = 0.12 s-1; printed kcat/Km = 0.26 mM-1 s-1.

table_label: Table 3: Kinetic parameters of the SgfSL variants (Int. J. Mol. Sci. 2019, volume 20, article 3184, page 6); printed column heads Substrate | Protein | Km (mM) | kcat (s-1) | kcat/Km (mM-1 s-1) | Activity nM O2 min-1 ug-1


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:A0A0M4FJ81 and applied mutation signature H165A to reconstruct the assayed variant sequence.

column_label: Table 3 (Kinetic parameters of the SgfSL variants), 2,6-DMP block, protein row 'H165A'; column 'Km (mM)' = 6.10 ± 0.34; column 'kcat (s-1)' = 1.10 ± 0.06; column 'kcat/Km (mM-1 s-1)' = 0.18.

normalized_value_unit_evidence: kcat: printed Table 3 cell 1.10 under the column head kcat (s-1); the cell is already in s^-1, so no unit conversion applies and the canonical value is 1.1 s^(-1). Km: printed Table 3 cell 6.10 under the column head Km (mM); the cell is already in mM, so no unit conversion applies and the canonical value is 6.1 mM. kcat/Km: printed Table 3 cell 0.18 under the column head kcat/Km (mM-1 s-1); the cell is already in mM^-1 s^-1, so no unit conversion applies and the printed cell carries over as 0.18 s^-1 mM^-1, while the full-precision quotient of the same line's two printed component cells is 1.10 / 6.10 = 0.18032786885245905 s^-1 mM^-1.

raw_value_unit_evidence: Table 3, 2,6-DMP block, protein row 'H165A': Km = 6.10 mM; kcat = 1.10 s-1; printed kcat/Km = 0.18 mM-1 s-1.


sequence_source: UniProtKB REST accession A0A0M4FJ81 checked 2026-05-31; I170F reconstructed from canonical sequence

sequence_variant_note: Mutation signature I170F matches the mutant residue at offset +0 and the reference residue at offset +9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


column_label: Table 3 (Kinetic parameters of the SgfSL variants), ABTS block, protein row 'H165A'; column 'Km (mM)' = 0.12 ± 0.01; column 'kcat (s-1)' = 10.80 ± 0.21; column 'kcat/Km (mM-1 s-1)' = 90.00; column 'Activity nM O2 min-1 ug-1' = 1.45 ± 0.10.

normalized_value_unit_evidence: kcat: printed Table 3 cell 10.80 under the column head kcat (s-1); the cell is already in s^-1, so no unit conversion applies and the canonical value is 10.8 s^(-1). Km: printed Table 3 cell 0.12 under the column head Km (mM); the cell is already in mM, so no unit conversion applies and the canonical value is 0.12 mM. kcat/Km: printed Table 3 cell 90.00 under the column head kcat/Km (mM-1 s-1); the cell is already in mM^-1 s^-1, so no unit conversion applies and the canonical value is 90.0 s^-1 mM^-1.

raw_value_unit_evidence: Table 3, ABTS block, protein row 'H165A': Km = 0.12 mM; kcat = 10.80 s-1; printed kcat/Km = 90.00 mM-1 s-1.


organism: Bacillus subtilis

sequence_source: UniProtKB:H8WGE7 REST lookup, checked 2026-05-31


sequence_variant_note: Resolved canonical sequence from UniProtKB:P07788 canonical sequence reconstructed with L431F, checked 2026-05-31 and applied mutation signature L431F to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 7; 313.15 K; buffer ethanol plus 100 mM citrate-phosphate


sequence_variant_note: Resolved canonical sequence from UniProtKB:P07788 canonical sequence reconstructed with R146K, checked 2026-05-31 and applied mutation signature R146K to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 4; 313.15 K; buffer pH 4


sequence_variant_note: Resolved canonical sequence from UniProtKB:P07788 canonical sequence reconstructed with R476K, checked 2026-05-31 and applied mutation signature R476K to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P07788 canonical sequence reconstructed with T480A, checked 2026-05-31 and applied mutation signature T480A to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:P07788 canonical sequence reconstructed with L431F, checked 2026-05-31


sequence_source: UniProtKB A0A0M4FJ81 canonical sequence fetched 2026-05-31; H165A and R240H applied after direct residue-position check.


sequence_source: UniProtKB A0A0M4FJ81 canonical sequence fetched 2026-05-31; H165A and M199G applied after direct residue-position check.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:A0A0M4FJ81 and applied mutation signature M199G to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 4; 303.15 K; buffer 50 mM Britton-Robinson

column_label: Table 2: Kinetic constants and pHopt of ABTS, 2.6-DMP, and K4[Fe(CN)6] oxidation by SgfSL-mutant forms and SgfSLwt (Int J Mol Sci 2022, 23(1):65), substrate block 'ABTS', protein row 'Met199Gly'; column 'pHopt' = 4.0; column 'Km(mM)' = 0.20 ± 0.06; column 'kcat (s-1)' = 45 ± 7; column 'kcat/Km(s-1 mM-1)' = 226.

normalized_value_unit_evidence: Km: printed Table 2 cell 0.20 mM; the printed unit is already mM, so no conversion applies and the paper value is 0.2 mM. kcat: printed Table 2 cell 45 s^-1; the printed unit is already s^-1, so no conversion applies and the paper value is 45.0 s^(-1). kcat/Km: printed Table 2 cell 226 s^-1 mM^-1; the printed unit is already s^-1 mM^-1, so no conversion applies and the paper value is 226.0 s^(-1)*mM^(-1). Closure disclosure: the quotient of the two printed component cells is 45 / 0.20 = 225.0 s^-1 mM^-1, which is the value the export stores for kcat_over_km (225.0); the paper's own printed efficiency cell is 226.0 s^-1 mM^-1, computed by the authors from their unrounded fitted parameters, and this record carries the printed cell.

raw_value_unit_evidence: Table 2, substrate block 'ABTS', protein row 'Met199Gly': Km = 0.20 ± 0.06 mM; kcat = 45 ± 7 s^-1; printed kcat/Km = 226 s^-1 mM^-1; printed pHopt = 4.0.


sequence_source: UniProtKB A0A0M4FJ81 canonical sequence fetched 2026-05-31.

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

column_label: Table 2: Kinetic constants and pHopt of ABTS, 2.6-DMP, and K4[Fe(CN)6] oxidation by SgfSL-mutant forms and SgfSLwt (Int J Mol Sci 2022, 23(1):65), substrate block 'K4[Fe(CN)6]', protein row 'SgfSLwt'; column 'pHopt' = 4.0; column 'Km(mM)' = 0.10 ± 0.02; column 'kcat (s-1)' = 38 ± 2; column 'kcat/Km(s-1 mM-1)' = 383.

normalized_value_unit_evidence: Km: printed Table 2 cell 0.10 mM; the printed unit is already mM, so no conversion applies and the paper value is 0.1 mM. kcat: printed Table 2 cell 38 s^-1; the printed unit is already s^-1, so no conversion applies and the paper value is 38.0 s^(-1). kcat/Km: printed Table 2 cell 383 s^-1 mM^-1; the printed unit is already s^-1 mM^-1, so no conversion applies and the paper value is 383.0 s^(-1)*mM^(-1). Closure disclosure: the quotient of the two printed component cells is 38 / 0.10 = 380.0 s^-1 mM^-1, which is the value the export stores for kcat_over_km (380.0); the paper's own printed efficiency cell is 383.0 s^-1 mM^-1, computed by the authors from their unrounded fitted parameters, and this record carries the printed cell.

raw_value_unit_evidence: Table 2, substrate block 'K4[Fe(CN)6]', protein row 'SgfSLwt': Km = 0.10 ± 0.02 mM; kcat = 38 ± 2 s^-1; printed kcat/Km = 383 s^-1 mM^-1; printed pHopt = 4.0.


organism: Streptomyces ipomoeae

sequence_source: UniProtKB A3QVH2 canonical sequence fetched 2026-05-31.


sequence_source: UniProt Q9XAL8 direct REST/FASTA check; RCSB 3CG8 SIFTS confirms Q9XAL8 alignment.


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt Q9XAL8 canonical sequence by direct S292A substitution; full-length residue 292 is S. and applied mutation signature S292A to reconstruct the assayed variant sequence.


substrate_name: quercetin

substrate: quercetin


sequence_source: Reconstructed from UniProt Q9XAL8 canonical sequence by direct S292A substitution; full-length residue 292 is S.


substrate_name: myricetin

substrate: myricetin


organism: Streptomyces sviceus

sequence_source: UniProt B5HSR1 FASTA; M220L applied after direct residue check showed canonical residue 220 is M.

sequence_variant_note: Mutation signature M220L matches the mutant residue at offset +0 and the reference residue at offset +12; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProt B5HSR1 FASTA; M195L applied after direct residue check showed canonical residue 195 is M.


sequence_source: UniProt B5HSR1 FASTA; M220L and T222L applied after direct residue checks showed canonical residues 220=M and 222=T.

sequence_variant_note: Mutation signature M220L/T222L matches the mutant residue at offset +0 and the reference residue at offset +24; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProt B5HSR1 FASTA; M293L applied after direct residue check showed canonical residue 293 is M.


substrate_name: Syringaldazine

sequence_source: UniProt B5HSR1 FASTA; M195A, V287N, M293G, and M295L applied after direct residue checks showed canonical residues 195=M, 287=V, 293=M, and 295=M.

substrate: Syringaldazine


sequence_source: UniProt B5HSR1 FASTA; M295V applied after direct residue check showed canonical residue 295 is M.


sequence_source: UniProt B5HSR1 FASTA; M293G applied after direct residue check showed canonical residue 293 is M.


sequence_source: UniProt B5HSR1 FASTA; M295T applied after direct residue check showed canonical residue 295 is M.


enzyme_display_name: Copper oxidase

sequence_source: UniProtKB B5HSR1 FASTA fetched 2026-05-31; M293G/M295L reconstructed from validated canonical residue(s): M293G validated against canonical residue M293; M295L validated against canonical residue M295.

enzyme: Copper oxidase


sequence_source: UniProtKB B5HSR1 FASTA fetched 2026-05-31; M295A reconstructed from validated canonical residue(s): M295A validated against canonical residue M295.


sequence_source: UniProtKB B5HSR1 FASTA fetched 2026-05-31; M295F reconstructed from validated canonical residue(s): M295F validated against canonical residue M295.


sequence_source: UniProtKB B5HSR1 FASTA fetched 2026-05-31; M295Y reconstructed from validated canonical residue(s): M295Y validated against canonical residue M295.


sequence_source: UniProtKB B5HSR1 FASTA fetched 2026-05-31; M295I reconstructed from validated canonical residue(s): M295I validated against canonical residue M295.


sequence_source: UniProtKB B5HSR1 FASTA fetched 2026-05-31; M295L reconstructed from validated canonical residue(s): M295L validated against canonical residue M295.


sequence_source: UniProtKB B5HSR1 FASTA fetched 2026-05-31; V287N reconstructed from validated canonical residue(s): V287N validated against canonical residue V287.


sequence_source: UniProtKB B5HSR1 FASTA fetched 2026-05-31; M195A/M293G/M295L reconstructed from validated canonical residue(s): M195A validated against canonical residue M195; M293G validated against canonical residue M293; M295L validated against canonical residue M295.


sequence_variant_note: Resolved canonical sequence from UniProtKB A0A0M4FJ81 FASTA; reconstructed with I170A and applied mutation signature I170A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB A0A0M4FJ81 FASTA; reconstructed with M199A and applied mutation signature M199A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB A0A0M4FJ81 FASTA; reconstructed with M199G/R240H and applied mutation signature M199G/R240H to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB A0A0M4FJ81 FASTA; reconstructed with M199G and applied mutation signature M199G to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB A0A0M4FJ81 FASTA; reconstructed with H165A/R240H and applied mutation signature H165A/R240H to reconstruct the assayed variant sequence.


sequence_source: UniProtKB A0A0M4FJ81 FASTA; reconstructed with M199A


sequence_source: UniProtKB A0A0M4FJ81 FASTA; reconstructed with H165A/R240H


sequence_variant_note: Resolved canonical sequence from UniProtKB A0A0M4FJ81 FASTA; reconstructed with Y230A and applied mutation signature Y230A to reconstruct the assayed variant sequence.


organism: Thermus thermophilus

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


substrate_name: catechol

substrate: catechol


sequence_source: UniProt:F6DF14 canonical sequence; D106L reconstructed on 2026-05-31 by replacing canonical residue 106 D with L from the selected source mutation signature.

sequence_variant_note: Mutation signature D106L matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B5HSR1 direct accession lookup; row-specific M195A sequence reconstructed from the canonical sequence. and applied mutation signature M195A to reconstruct the assayed variant sequence.


organism: Streptomyces viridochromogenes

sequence_source: UniProtKB:J9S5G3 direct accession lookup.


organism: Thermobaculum terrenum


organism: Thermobifida fusca

sequence_source: UniProt Q47QW5 / NCBI Protein AAZ55152.1 canonical sequence with R49K reconstructed after confirming residue 49 is R.

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


organism: Thermus sp.

sequence_source: NCBI Protein KHG66454.1 from the PMID 30756202 paper-linked Thermus sp. 2.9 LAC_2.9 route; source UniProt A0A0B0SEJ6 is inactive.


sequence_source: UniProt Q4H436 / NCBI Protein BAE16261.1 canonical wild-type sequence.


sequence_source: UniProt F6DF14 / NCBI Protein AEG34143.1 canonical wild-type sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0M4FJ81; direct REST/FASTA check 2026-05-31 and applied mutation signature Y230A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0M4FJ81; direct REST/FASTA check 2026-05-31 and applied mutation signature M199G/R240H to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:A0A0M4FJ81; direct REST/FASTA check 2026-05-31


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0M4FJ81; direct REST/FASTA check 2026-05-31 and applied mutation signature M199A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0M4FJ81; direct REST/FASTA check 2026-05-31 and applied mutation signature M199G to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:F6DF14 direct lookup; mutant sequence reconstructed from selected mutation_signature when present and applied mutation signature D394N to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:F6DF14 direct lookup; mutant sequence reconstructed from selected mutation_signature when present and applied mutation signature D394E to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:F6DF14 direct lookup; mutant sequence reconstructed from selected mutation_signature when present

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


sequence_variant_note: Resolved canonical sequence from UniProtKB:F6DF14 direct lookup; mutant sequence reconstructed from selected mutation_signature when present and applied mutation signature D394R to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:F6DF14 direct lookup; mutant sequence reconstructed from selected mutation_signature when present and applied mutation signature D396M to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:F6DF14 direct lookup; mutant sequence reconstructed from selected mutation_signature when present and applied mutation signature D396A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:F6DF14 direct lookup; mutant sequence reconstructed from selected mutation_signature when present and applied mutation signature D394M to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:F6DF14 direct lookup; mutant sequence reconstructed from selected mutation_signature when present and applied mutation signature D396N to reconstruct the assayed variant sequence.


organism: Toxicodendron vernicifluum

substrate_name: ferrocyanide

substrate: ferrocyanide


substrate_name: dopamine

substrate: dopamine


substrate_name: ferrocenemonocarboxylic acid

substrate: ferrocenemonocarboxylic acid


organism: Trametes hirsuta


substrate_name: Eugenol

substrate: Eugenol


organism: Trametes sp.


organism: Trametes sp. C30


sequence_source: UniProtKB Q02497 direct REST lookup; LAC1_TRAHI reviewed laccase, Trametes hirsuta, EC 1.10.3.2, sequence length 520.


sequence_source: UniProtKB B2L9C1 direct REST lookup; B2L9C1_TRAHI unreviewed laccase, Trametes hirsuta, EC 1.10.3.2, sequence length 520.


substrate_name: hydroquinone

substrate: hydroquinone


substrate_name: Potassium ferrocyanide

substrate: Potassium ferrocyanide


organism: Streptomyces sp. C1

substrate_name: L-Dopa

substrate: L-Dopa


assay_conditions_summary: pH 9; 295.15 K; buffer pH 9


assay_conditions_summary: pH 8; 295.15 K; buffer pH 8


assay_conditions_summary: pH 4; 295.15 K; buffer pH 4


sequence_variant_note: Resolved canonical sequence from uniprot:B5HSR1 plus M295L reconstruction and applied mutation signature M295L to reconstruct the assayed variant sequence.


sequence_source: Reconstructed from UniProt F6DF14 canonical sequence after direct residue check: position 106 is D and was changed to R.

sequence_variant_note: Mutation signature D106R matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:F6DF14 and applied mutation signature E456L to reconstruct the assayed variant sequence.

column_label: Table 1: Apparent steady-state kinetic constants measured at the saturating concentrations of O2 for guaiacol by the lacTT proteins (Appl Microbiol Biotechnol 2018, 102:4049-4061); printed column heads LacTT | Km (uM) | kcat (s^-1) | kcat/Km (s^-1 mM^-1); enzyme row E456L; column 'Km (uM)' = 135; column 'kcat (s^-1)' = 0.059; column 'kcat/Km (s^-1 mM^-1)' = 0.44. captured primary segment: Table 1 row E456L | construct lacTT E456L | substrate guaiacol | condition pH 6.0 | Km 135 uM | kcat 0.059 s^-1 | kcat/Km 0.44 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 1 cell 135 uM; 135 / 1000 = 0.135 mM. kcat: printed Table 1 cell 0.059 s^-1; the printed turnover column is per second, so no unit conversion applies and the value is 0.059 s^(-1). kcat/Km: printed Table 1 cell 0.44 s^-1 mM^-1; the printed efficiency column is already per second per millimolar, so no unit conversion applies and the value is 0.44 s^(-1)*mM^(-1). Closure disclosure for this row: the quotient of the two printed component cells is 0.059 / 0.135 = 0.437037037037037 s^-1 mM^-1, which is the exported catalytic efficiency, and the paper's own printed efficiency cell 0.44 s^-1 mM^-1 is that same quotient written at the table's printed precision.

raw_value_unit_evidence: Table 1, enzyme row E456L: Km = 135 uM; kcat = 0.059 s^-1; kcat/Km = 0.44 s^-1 mM^-1.

row_label: Table 1, enzyme row E456L


sequence_source: Reconstructed from UniProt F6DF14 canonical sequence after direct residue check: position 106 is D and was changed to E.

sequence_variant_note: Mutation signature D106E matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:F6DF14 and applied mutation signature E456D to reconstruct the assayed variant sequence.

column_label: Table 1: Apparent steady-state kinetic constants measured at the saturating concentrations of O2 for guaiacol by the lacTT proteins (Appl Microbiol Biotechnol 2018, 102:4049-4061); printed column heads LacTT | Km (uM) | kcat (s^-1) | kcat/Km (s^-1 mM^-1); enzyme row E456D; column 'Km (uM)' = 380; column 'kcat (s^-1)' = 0.78; column 'kcat/Km (s^-1 mM^-1)' = 2.1. captured primary segment: Table 1 row E456D | construct lacTT E456D | substrate guaiacol | condition pH 6.0 | Km 380 uM | kcat 0.78 s^-1 | kcat/Km 2.1 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 1 cell 380 uM; 380 / 1000 = 0.38 mM. kcat: printed Table 1 cell 0.78 s^-1; the printed turnover column is per second, so no unit conversion applies and the value is 0.78 s^(-1). kcat/Km: printed Table 1 cell 2.1 s^-1 mM^-1; the printed efficiency column is already per second per millimolar, so no unit conversion applies and the value is 2.1 s^(-1)*mM^(-1). Closure disclosure for this row: the quotient of the two printed component cells is 0.78 / 0.38 = 2.0526315789473686 s^-1 mM^-1, which is the exported catalytic efficiency, and the paper's own printed efficiency cell 2.1 s^-1 mM^-1 is that same quotient written at the table's printed precision.

raw_value_unit_evidence: Table 1, enzyme row E456D: Km = 380 uM; kcat = 0.78 s^-1; kcat/Km = 2.1 s^-1 mM^-1.

row_label: Table 1, enzyme row E456D


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:F6DF14 and applied mutation signature E456R to reconstruct the assayed variant sequence.

column_label: Table 1: Apparent steady-state kinetic constants measured at the saturating concentrations of O2 for guaiacol by the lacTT proteins (Appl Microbiol Biotechnol 2018, 102:4049-4061); printed column heads LacTT | Km (uM) | kcat (s^-1) | kcat/Km (s^-1 mM^-1); enzyme row E456R; column 'Km (uM)' = 512; column 'kcat (s^-1)' = 0.32; column 'kcat/Km (s^-1 mM^-1)' = 0.62. captured primary segment: Table 1 row E456R | construct lacTT E456R | substrate guaiacol | condition pH 6.0 | Km 512 uM | kcat 0.32 s^-1 | kcat/Km 0.62 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 1 cell 512 uM; 512 / 1000 = 0.512 mM. kcat: printed Table 1 cell 0.32 s^-1; the printed turnover column is per second, so no unit conversion applies and the value is 0.32 s^(-1). kcat/Km: printed Table 1 cell 0.62 s^-1 mM^-1; the printed efficiency column is already per second per millimolar, so no unit conversion applies and the value is 0.62 s^(-1)*mM^(-1). Quotient disclosure for this row: 0.32 / 0.512 = 0.625 s^-1 mM^-1, against the paper's own printed efficiency cell 0.62 s^-1 mM^-1, which is the value carried forward here.

raw_value_unit_evidence: Table 1, enzyme row E456R: Km = 512 uM; kcat = 0.32 s^-1; kcat/Km = 0.62 s^-1 mM^-1.

row_label: Table 1, enzyme row E456R


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:F6DF14 and applied mutation signature D423A to reconstruct the assayed variant sequence.

column_label: Table 1: Apparent steady-state kinetic constants measured at the saturating concentrations of O2 for guaiacol by the lacTT proteins (Appl Microbiol Biotechnol 2018, 102:4049-4061); printed column heads LacTT | Km (uM) | kcat (s^-1) | kcat/Km (s^-1 mM^-1); enzyme row D423A; column 'Km (uM)' = 476; column 'kcat (s^-1)' = 3.51; column 'kcat/Km (s^-1 mM^-1)' = 7.37. captured primary segment: Table 1 row D423A | construct lacTT D423A | substrate guaiacol | condition pH 6.0 | Km 476 uM | kcat 3.51 s^-1 | kcat/Km 7.37 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 1 cell 476 uM; 476 / 1000 = 0.476 mM. kcat: printed Table 1 cell 3.51 s^-1; the printed turnover column is per second, so no unit conversion applies and the value is 3.51 s^(-1). kcat/Km: printed Table 1 cell 7.37 s^-1 mM^-1; the printed efficiency column is already per second per millimolar, so no unit conversion applies and the value is 7.37 s^(-1)*mM^(-1). Closure disclosure for this row: the quotient of the two printed component cells is 3.51 / 0.476 = 7.373949579831932 s^-1 mM^-1, which is the exported catalytic efficiency, and the paper's own printed efficiency cell 7.37 s^-1 mM^-1 is that same quotient written at the table's printed precision.

raw_value_unit_evidence: Table 1, enzyme row D423A: Km = 476 uM; kcat = 3.51 s^-1; kcat/Km = 7.37 s^-1 mM^-1.

row_label: Table 1, enzyme row D423A


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:F6DF14 and applied mutation signature E356A to reconstruct the assayed variant sequence.

column_label: Table 1: Apparent steady-state kinetic constants measured at the saturating concentrations of O2 for guaiacol by the lacTT proteins (Appl Microbiol Biotechnol 2018, 102:4049-4061); printed column heads LacTT | Km (uM) | kcat (s^-1) | kcat/Km (s^-1 mM^-1); enzyme row E356A; column 'Km (uM)' = 1466; column 'kcat (s^-1)' = 1.70; column 'kcat/Km (s^-1 mM^-1)' = 1.16. captured primary segment: Table 1 row E356A | construct lacTT E356A | substrate guaiacol | condition pH 6.0 | Km 1466 uM | kcat 1.70 s^-1 | kcat/Km 1.16 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 1 cell 1466 uM; 1466 / 1000 = 1.466 mM. kcat: printed Table 1 cell 1.70 s^-1; the printed turnover column is per second, so no unit conversion applies and the value is 1.7 s^(-1). kcat/Km: printed Table 1 cell 1.16 s^-1 mM^-1; the printed efficiency column is already per second per millimolar, so no unit conversion applies and the value is 1.16 s^(-1)*mM^(-1). Closure disclosure for this row: the quotient of the two printed component cells is 1.7 / 1.466 = 1.159618008185539 s^-1 mM^-1, which is the exported catalytic efficiency, and the paper's own printed efficiency cell 1.16 s^-1 mM^-1 is that same quotient written at the table's printed precision.

raw_value_unit_evidence: Table 1, enzyme row E356A: Km = 1466 uM; kcat = 1.70 s^-1; kcat/Km = 1.16 s^-1 mM^-1.

row_label: Table 1, enzyme row E356A


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt F6DF14 canonical sequence after direct residue check: position 460 is M and was changed to Q. and applied mutation signature M460Q to reconstruct the assayed variant sequence.


sequence_source: UniProtKB exact accession Q8NID5 direct lookup


sequence_source: UniProtKB exact accession Q6TH77 direct lookup


organism: Trametes versicolor

substrate_name: Methyl syringate

sequence_source: UniProtKB exact accession Q12718 direct lookup

substrate: Methyl syringate


substrate_name: acetosyringone

substrate: acetosyringone


substrate_name: syringaldehyde

substrate: syringaldehyde


organism: unknown prokaryotic organism

sequence_source: UniProtKB exact accession Q1EIR0 direct lookup


sequence_source: UniProtKB Q1EIR0 direct FASTA/JSON lookup on 2026-05-31


sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


substrate_name: alpha-Naphthol

substrate: alpha-Naphthol


sequence_source: UniProtKB A0ACD6B8I0, direct live replacement for inactive/demerged E1ACR6


sequence_variant_note: Resolved canonical sequence from UniProtKB:F6DF14 and applied mutation signature M460H to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:F6DF14 and applied mutation signature M460L to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:F6DF14 and applied mutation signature M460A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:F6DF14 and applied mutation signature M460F to reconstruct the assayed variant sequence.


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q72HW2 and applied mutation signature M455L to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q72HW2 and applied mutation signature M456A to reconstruct the assayed variant sequence.


substrate_name: guaiacol

sequence_variant_note: Resolved canonical sequence from UniProtKB:F6DF14 and applied mutation signature D396E to reconstruct the assayed variant sequence.

substrate: guaiacol


sequence_source: NCBI GenBank KP752045.1 CDS translation, protein_id AKN79754.1; source UniProt A0A0H4CVT8 is inactive/deleted and was not promoted

assay_conditions_summary: pH 7; 333.15 K; buffer pH 7


substrate_name: Guaiacol

substrate: Guaiacol


enzyme_display_name: phosphoglycerate kinase

organism: Homo sapiens

substrate_name: ADP

sequence_source: UniProtKB P00558 canonical PGK1_HUMAN sequence retrieved directly on 2026-05-31; validated source residue E at 1-based position 344 and applied E344A substitution.

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ADP

enzyme: phosphoglycerate kinase

substrate: ADP


substrate_name: dADP

sequence_source: UniProtKB P00558 canonical PGK1_HUMAN sequence retrieved directly on 2026-05-31; validated source residue N at 1-based position 337 and applied N337D substitution.

substrate: dADP


substrate_name: dATP

sequence_source: UniProtKB P00558 canonical PGK1_HUMAN FASTA/JSON retrieved directly on 2026-05-31.

column_label: PMID 12080078 Table II Dephosphorylation of nucleoside analog triphosphates by PGK; row dATP (Table II); column headers Km and kcat with the table's own printed units line (micromolar Km and min^-1 kcat); printed cells Km 73.6 uM, kcat 1.8 min^-1. The exported kcat/Km is recorded as the quotient of this same row's own two printed cells as stored (0.03 s^-1 / 0.074 mM = 0.40540540540540543 s^-1 mM^-1); derived from this same row's own two printed cells; Table II's third numeric column is the reverse-versus-forward efficiency comparison named by its own column header and footnote 2-a, not a kcat/Km cell for this row, so no printed kcat/Km cell exists for this row and the third column is not used.

raw_value_unit_evidence: Table row as printed: Km 73.6 uM; kcat 1.8 min^-1 (Table II, wild-type PGK).

row_label: dATP (Table II)

substrate: dATP


substrate_name: ATP

sequence_source: UniProtKB P00558 canonical PGK1_HUMAN sequence retrieved directly on 2026-05-31; validated source residue K at 1-based position 220 and applied K220A substitution.

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

substrate: ATP


substrate_name: dCTP

substrate: dCTP


substrate_name: dTTP

substrate: dTTP


substrate_name: CTP

substrate: CTP


sequence_source: UniProtKB P00558 canonical PGK1_HUMAN sequence retrieved directly on 2026-05-31; validated source residue N at 1-based position 337 and applied N337Q substitution.


sequence_source: UniProtKB:P00558 direct REST lookup (reviewed Swiss-Prot canonical sequence)

column_label: PMID 12080078 Table II Dephosphorylation of nucleoside analog triphosphates by PGK; row CTP (Table II); column headers Km and kcat with the table's own printed units line (micromolar Km and min^-1 kcat); printed cells Km 86.7 uM, kcat 0.5 min^-1. The exported kcat/Km is recorded as the quotient of this same row's own two printed cells as stored (0.008 s^-1 / 0.087 mM = 0.09195402298850576 s^-1 mM^-1); derived from this same row's own two printed cells; Table II's third numeric column is the reverse-versus-forward efficiency comparison named by its own column header and footnote 2-a, not a kcat/Km cell for this row, so no printed kcat/Km cell exists for this row and the third column is not used.

raw_value_unit_evidence: Table row as printed: Km 86.7 uM; kcat 0.5 min^-1 (Table II, wild-type PGK).

row_label: CTP (Table II)


sequence_source: reconstructed from UniProtKB:P00558 canonical sequence by K220R


substrate_name: CDP

column_label: PMID 12080078 Table I Phosphorylation of nucleoside analog diphosphates by PGK; row CDP (Table I); column headers Km and kcat with the table's own printed units line (micromolar Km and s^-1 kcat); printed cells Km 885.0 uM, kcat 0.9 s^-1. The exported kcat/Km is recorded as the quotient of this same row's own two printed cells as stored (0.9 s^-1 / 0.885 mM = 1.0169491525423728 s^-1 mM^-1); derived from this same row's own two printed cells; Table I prints a Km column and a kcat column only and carries no kcat/Km column, so no printed ratio cell exists for this row.

raw_value_unit_evidence: Table row as printed: Km 885.0 uM; kcat 0.9 s^-1 (Table I, wild-type PGK).

row_label: CDP (Table I)

substrate: CDP


sequence_source: reconstructed from UniProtKB:P00558 canonical sequence by K220A


substrate_name: ddCTP

sequence_source: reconstructed from UniProtKB:P00558 canonical sequence by N337Q

substrate: ddCTP


sequence_source: reconstructed from UniProtKB:P00558 canonical sequence by N337D


column_label: PMID 12080078 Table II Dephosphorylation of nucleoside analog triphosphates by PGK; row ddCTP (Table II); column headers Km and kcat with the table's own printed units line (micromolar Km and min^-1 kcat); printed cells Km 692.8 uM, kcat 0.9 min^-1. The exported kcat/Km is recorded as the quotient of this same row's own two printed cells as stored (0.015 s^-1 / 0.693 mM = 0.021645021645021644 s^-1 mM^-1); derived from this same row's own two printed cells; Table II's third numeric column is the reverse-versus-forward efficiency comparison named by its own column header and footnote 2-a, not a kcat/Km cell for this row, so no printed kcat/Km cell exists for this row and the third column is not used.

raw_value_unit_evidence: Table row as printed: Km 692.8 uM; kcat 0.9 min^-1 (Table II, wild-type PGK).

row_label: ddCTP (Table II)


sequence_variant_note: Resolved canonical sequence from UniProtKB:P00558 canonical sequence fetched by REST/FASTA on 2026-05-31; K220A reconstructed after direct residue check K220. and applied mutation signature K220A to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:P00558 canonical sequence fetched by REST/FASTA on 2026-05-31; K220A reconstructed after direct residue check K220.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P00558 canonical sequence fetched by REST/FASTA on 2026-05-31; K220R reconstructed after direct residue check K220. and applied mutation signature K220R to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:P00558 canonical sequence fetched by REST/FASTA on 2026-05-31; K220R reconstructed after direct residue check K220.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P00558 canonical sequence fetched by REST/FASTA on 2026-05-31; N337D reconstructed after direct residue check N337. and applied mutation signature N337D to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:P00558 reviewed PGK1_HUMAN canonical sequence

column_label: PMID 12080078 Table II Dephosphorylation of nucleoside analog triphosphates by PGK; row TTP (Table II); column headers Km and kcat with the table's own printed units line (micromolar Km and min^-1 kcat); printed cells Km 101.8 uM, kcat 1.0 min^-1. The exported kcat/Km is recorded as the quotient of this same row's own two printed cells as stored (0.017 s^-1 / 0.102 mM = 0.16666666666666669 s^-1 mM^-1); derived from this same row's own two printed cells; Table II's third numeric column is the reverse-versus-forward efficiency comparison named by its own column header and footnote 2-a, not a kcat/Km cell for this row, so no printed kcat/Km cell exists for this row and the third column is not used.

raw_value_unit_evidence: Table row as printed: Km 101.8 uM; kcat 1.0 min^-1 (Table II, wild-type PGK).

row_label: TTP (Table II)


column_label: PMID 12080078 Table I Phosphorylation of nucleoside analog diphosphates by PGK; row ADP (Table I); column headers Km and kcat with the table's own printed units line (micromolar Km and s^-1 kcat); printed cells Km 232.5 uM, kcat 6000.0 s^-1. The exported kcat/Km is recorded as the quotient of this same row's own two printed cells as stored (6000.0 s^-1 / 0.233 mM = 25751.07296137339 s^-1 mM^-1); derived from this same row's own two printed cells; Table I prints a Km column and a kcat column only and carries no kcat/Km column, so no printed ratio cell exists for this row.

raw_value_unit_evidence: Table row as printed: Km 232.5 uM; kcat 6000.0 s^-1 (Table I, wild-type PGK).

row_label: ADP (Table I)


sequence_source: UniProtKB:P00558 reviewed PGK1_HUMAN; variant reconstructed from selected OED mutation signature


substrate_name: TDP

substrate: TDP


organism: Saccharomyces cerevisiae

sequence_source: UniProt P00560 canonical sequence; row-specific variant reconstructed using PMID 8462685 mutation signature and UniProt initiator-methionine removal feature

sequence_variant_note: Mutation signature R121M matches the mutant residue at offset +1 and the reference residue at offset +10; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

assay_conditions_summary: pH 7.5; 298.15 K; cofactor ATP


sequence_variant_note: Resolved canonical sequence from UniProt P00560 canonical sequence; row-specific variant reconstructed using PMID 8462685 mutation signature and UniProt initiator-methionine removal feature and applied mutation signature R168M with inferred residue-numbering offset +14 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt P00560 canonical sequence; row-specific variant reconstructed using PMID 8462685 mutation signature and UniProt initiator-methionine removal feature and applied mutation signature R121K with inferred residue-numbering offset +10 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset +1, so it is treated as a source-provided variant sequence.


sequence_variant_note: Mutation signature R168K matches the mutant residue at offset +1 and the reference residue at offset +14; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


organism: Rattus norvegicus

sequence_source: UniProtKB reviewed accession P16617 FASTA, retrieved 2026-05-31

assay_conditions_summary: pH 7.3; 303.15 K; cofactor NADH

column_label: Literal Table II backward 3-phosphoglycerate row. Current Kcat 3.983 and OED source efficiency select the native Kcat 239 and native Km 2.22 cells; current 2.72 Km is the modified cell and is corrected.

normalized_value_unit_evidence: 239 min^-1 is stored at source row precision as 3.983 s^-1; native Km=2.22 mM; corrected same-native-column quotient=3.983/2.22=1.7941441441441441 s^-1 mM^-1.

raw_value_unit_evidence: Table II native 3-phosphoglycerate cells: Kcat 239 min^-1 and Km,app 2.22 mM. Modified Km 2.72 mM is a distinct preparation cell.

row_label: backward direction; 3-phosphoglycerate; native

table_label: Table II. Kinetic Constants of Native and Iodoacetate-Modified Phosphoglycerate Kinase


assay_conditions_summary: pH 6.9; 303.15 K; cofactor ADP


substrate_name: Glycerate 3-phosphate

sequence_source: UniProtKB reviewed accession P00560 FASTA, retrieved 2026-05-31

smiles: O=C(O)C(O)COP(=O)(O)O

substrate: Glycerate 3-phosphate


substrate_name: 2-Hydroxy-4-phosphonobutyric acid

smiles: O=C(O)C(O)CCP(=O)(O)O

substrate: 2-Hydroxy-4-phosphonobutyric acid


assay_conditions_summary: pH 7.5; 277.15 K; cofactor ATP


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


organism: Chlamydomonas reinhardtii

sequence_source: UniProtKB:A8JC04 canonical sequence fetched directly


assay_conditions_summary: pH 7.9; 298.15 K; cofactor ATP


organism: Entamoeba histolytica

sequence_source: UniProtKB:C4M192 canonical sequence fetched directly

assay_conditions_summary: pH 7; 310.15 K; cofactor ATP


sequence_source: UniProtKB:P00558 canonical sequence fetched directly

column_label: PMID 12080078 Table I Phosphorylation of nucleoside analog diphosphates by PGK; row dADP (Table I); column headers Km and kcat with the table's own printed units line (micromolar Km and s^-1 kcat); printed cells Km 488.1 uM, kcat 844.5 s^-1. The exported kcat/Km is recorded as the quotient of this same row's own two printed cells as stored (844.5 s^-1 / 0.488 mM = 1730.532786885246 s^-1 mM^-1); derived from this same row's own two printed cells; Table I prints a Km column and a kcat column only and carries no kcat/Km column, so no printed ratio cell exists for this row.

raw_value_unit_evidence: Table row as printed: Km 488.1 uM; kcat 844.5 s^-1 (Table I, wild-type PGK).

row_label: dADP (Table I)


column_label: PMID 12080078 Table I Phosphorylation of nucleoside analog diphosphates by PGK; row TDP (Table I); column headers Km and kcat with the table's own printed units line (micromolar Km and s^-1 kcat); printed cells Km 160.5 uM, kcat 0.3 s^-1. The exported kcat/Km is recorded as the quotient of this same row's own two printed cells as stored (0.3 s^-1 / 0.161 mM = 1.8633540372670807 s^-1 mM^-1); derived from this same row's own two printed cells; Table I prints a Km column and a kcat column only and carries no kcat/Km column, so no printed ratio cell exists for this row.

raw_value_unit_evidence: Table row as printed: Km 160.5 uM; kcat 0.3 s^-1 (Table I, wild-type PGK).

row_label: TDP (Table I)


sequence_source: UniProtKB:P00558 canonical sequence fetched directly; reconstructed N337D after confirming N337


sequence_source: UniProtKB:P00558 canonical sequence fetched directly; reconstructed N337Q after confirming N337


sequence_source: UniProtKB:P00558 canonical sequence fetched directly; reconstructed K220A after confirming K220


sequence_source: UniProtKB:P00560 direct REST/FASTA fetch


sequence_source: UniProtKB reviewed P00558 fetched 2026-05-31; validated residue 220=K in UniProt P00558 before applying K220R.


sequence_source: UniProtKB reviewed P00558 fetched 2026-05-31; validated residue 337=N in UniProt P00558 before applying N337D.


sequence_source: UniProtKB reviewed P00558 fetched 2026-05-31; canonical wild-type sequence used without mutation reconstruction.

column_label: PMID 12080078 Table II Dephosphorylation of nucleoside analog triphosphates by PGK; row ATP (Table II); column headers Km and kcat with the table's own printed units line (micromolar Km and min^-1 kcat); printed cells Km 58.8 uM, kcat 0.9 min^-1. The exported kcat/Km is recorded as the quotient of this same row's own two printed cells as stored (0.015 s^-1 / 0.059 mM = 0.2542372881355932 s^-1 mM^-1); derived from this same row's own two printed cells; Table II's third numeric column is the reverse-versus-forward efficiency comparison named by its own column header and footnote 2-a, not a kcat/Km cell for this row, so no printed kcat/Km cell exists for this row and the third column is not used.

raw_value_unit_evidence: Table row as printed: Km 58.8 uM; kcat 0.9 min^-1 (Table II, wild-type PGK).

row_label: ATP (Table II)


sequence_source: UniProtKB reviewed P00558 fetched 2026-05-31; validated residue 337=N in UniProt P00558 before applying N337Q.


column_label: PMID 12080078 Table II Dephosphorylation of nucleoside analog triphosphates by PGK; row dCTP (Table II); column headers Km and kcat with the table's own printed units line (micromolar Km and min^-1 kcat); printed cells Km 109.8 uM, kcat 0.4 min^-1. The exported kcat/Km is recorded as the quotient of this same row's own two printed cells as stored (0.007 s^-1 / 0.11 mM = 0.06363636363636364 s^-1 mM^-1); derived from this same row's own two printed cells; Table II's third numeric column is the reverse-versus-forward efficiency comparison named by its own column header and footnote 2-a, not a kcat/Km cell for this row, so no printed kcat/Km cell exists for this row and the third column is not used.

raw_value_unit_evidence: Table row as printed: Km 109.8 uM; kcat 0.4 min^-1 (Table II, wild-type PGK).

row_label: dCTP (Table II)


sequence_variant_note: Resolved canonical sequence from uniprot_accession:P00558 and applied mutation signature A199V with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession:P00558 and applied mutation signature M189I with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


organism: Methanothermus fervidus


organism: Pseudomonas sp.


organism: Pyrococcus woesei

assay_conditions_summary: pH 7.5; 343.15 K; cofactor ATP


assay_conditions_summary: pH 7.3; 303.15 K; cofactor ATP

column_label: Literal Table II backward MgATP row, modified columns: Vmax 0.22, Km,app 0.91 mM, Kcat 548 min^-1. Footnotes define the iodoacetate preparation and Kcat.

normalized_value_unit_evidence: 548 min^-1 is stored at source row precision as 9.133 s^-1; Km=0.91 mM; same-modified-column quotient=9.133/0.91=10.036263736263734 s^-1 mM^-1.

raw_value_unit_evidence: Table II modified MgATP cells: Kcat 548 min^-1 and Km,app 0.91 mM. No kcat/Km cell is printed.

row_label: backward direction; MgATP; modified


substrate_name: 3-Phospho-D-glyceroyl phosphate

column_label: Literal Table II forward 1,3-diphosphoglycerate row. Current Kcat 25.683 and OED source efficiency select the modified Kcat 1541 and modified Km 0.036 cells; current 0.042 Km is the native cell and is corrected.

normalized_value_unit_evidence: 1541 min^-1 is stored at source row precision as 25.683 s^-1; modified Km=0.036 mM; corrected same-modified-column quotient=25.683/0.036=713.4166666666666 s^-1 mM^-1.

raw_value_unit_evidence: Table II modified 1,3-diphosphoglycerate cells: Kcat 1541 min^-1 and Km,app 0.036 mM. Native Km 0.042 mM is a distinct preparation cell.

row_label: forward direction; 1,3-diphosphoglycerate; modified

substrate: 3-Phospho-D-glyceroyl phosphate


sequence_source: UniProt:P00558 direct FASTA/JSON check

sequence_variant_note: Resolved canonical sequence from UniProt:P00558 direct FASTA/JSON check and applied mutation signature K220R to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt:P00558 direct FASTA/JSON check and applied mutation signature K215A with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt:P00558 direct FASTA/JSON check and applied mutation signature R38M with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt:P00558 direct FASTA/JSON check and applied mutation signature G166D with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

assay_conditions_summary: pH 8; 355.15 K; cofactor NADH

column_label: PMID 29995887 Table 1 row Wild type; columns KM (mM), kcat (s^-1), and kcat/KM (s^-1 mM^-1).

raw_value_unit_evidence: Table 1 row Wild type: KM 0.40 mM; kcat 89.8 s^-1; kcat/KM 224.5 s^-1 mM^-1.

row_label: Wild type

table_label: Table 1. Kinetic parameters of PGK1 wild type and variants


sequence_variant_note: Resolved canonical sequence from UniProt:P00558 direct FASTA/JSON check and applied mutation signature V216F with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt:P00558 direct FASTA/JSON check and applied mutation signature R65W with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt:P00558 direct FASTA/JSON check and applied mutation signature F241S with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.