column_label: Table 2. Kinetic parameters of wildtype and mutant glyceraldehyde 3-phosphate dehydrogenase; row M3(D35G/L36T/T37K/P192S) / NADP; columns Km (mM), Kcat (min^-1), Kcat/Km (mM^-1 min^-1); cells Km 0.070+/-0.003, Kcat 428+/-45, Kcat/Km 5868+/-352.

normalized_value_unit_evidence: Km normalized_value=0.07 normalized_unit=mM; kcat normalized_value=7.133333333333 normalized_unit=s^-1; kcat/Km normalized_value=97.8 normalized_unit=s^-1 mM^-1.


organism: Paraburkholderia xenovorans

substrate_name: acetaldehyde

source_db: oed

verification_status: corrected

ki_display: Not reported

protein_accession_database: UniProt

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: Resolved canonical sequence from uniprotkb_reviewed:Q79AF6 and applied mutation signature D208A to reconstruct the assayed variant sequence.

smiles: CC=O

mutation_type: substitution

assay_conditions_summary: pH 8; 298.15 K; cofactor NAD+

column_label: Official ACS publisher PDF, Table 1. Steady-State Kinetic Parameters of BphJ with Acetaldehyde as the Substrate, row D208A, substrate acetaldehyde, columns Km (mM), kcat (s^-1), and kcat/Km (M^-1 s^-1): 18.7 +/- 1.4; 7.6 +/- 0.2; 406 +/- 34.

normalized_value_unit_evidence: Km 18.7 mM; kcat 7.6 s^-1; printed kcat/Km 0.406 s^-1 mM^-1.

row_label: D208A — acetaldehyde

data_origin: experimental

substrate: acetaldehyde

status: corrected

source: oed


sequence_variant_note: Resolved canonical sequence from uniprotkb_reviewed:Q79AF6 and applied mutation signature I195A to reconstruct the assayed variant sequence.

column_label: Official ACS publisher PDF, Table 2. Substrate Specificity of BphJ in the Dehydrogenase Reaction, row I195A, substrate acetaldehyde, columns Km (mM), kcat (s^-1), and kcat/Km (M^-1 s^-1): 133 +/- 12; 19.7 +/- 0.9; 147 +/- 15.

normalized_value_unit_evidence: Km 133 mM; kcat 19.7 s^-1; printed kcat/Km 0.147 s^-1 mM^-1.

raw_value_unit_evidence: Table 2 I195A — acetaldehyde: Km 133 +/- 12 mM; kcat 19.7 +/- 0.9 s^-1; printed kcat/Km 147 +/- 15 M^-1 s^-1.

row_label: I195A — acetaldehyde


sequence_variant_note: Resolved canonical sequence from uniprotkb_reviewed:Q79AF6 and applied mutation signature I195F to reconstruct the assayed variant sequence.

column_label: Official ACS publisher PDF, Table 2. Substrate Specificity of BphJ in the Dehydrogenase Reaction, row I195F, substrate acetaldehyde, columns Km (mM), kcat (s^-1), and kcat/Km (M^-1 s^-1): 33.7 +/- 3.2; 15.4 +/- 0.6; 457 +/- 48.

row_label: I195F — acetaldehyde


mutation_signature: Unknown

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

column_label: Official ACS publisher PDF, Table 2. Substrate Specificity of BphJ in the Dehydrogenase Reaction, row WT, substrate acetaldehyde, columns Km (mM), kcat (s^-1), and kcat/Km (M^-1 s^-1): 23.6 +/- 1.8; 17.2 +/- 0.5; 730 +/- 60.

normalized_value_unit_evidence: Km 23.6 mM; kcat 17.2 s^-1; printed kcat/Km 0.73 s^-1 mM^-1.

raw_value_unit_evidence: Table 2 WT — acetaldehyde: Km 23.6 +/- 1.8 mM; kcat 17.2 +/- 0.5 s^-1; printed kcat/Km 730 +/- 60 M^-1 s^-1.

row_label: WT — acetaldehyde


substrate_name: Butyraldehyde

smiles: CCCC=O

column_label: Official ACS publisher PDF, Table 2. Substrate Specificity of BphJ in the Dehydrogenase Reaction, row I195A, substrate butyraldehyde, columns Km (mM), kcat (s^-1), and kcat/Km (M^-1 s^-1): 4.6 +/- 0.3; 12.6 +/- 0.3; 2763 +/- 181.

normalized_value_unit_evidence: Km 4.6 mM; kcat 12.6 s^-1; printed kcat/Km 2.763 s^-1 mM^-1.

row_label: I195A — butyraldehyde

substrate: Butyraldehyde


column_label: Official ACS publisher PDF, Table 2. Substrate Specificity of BphJ in the Dehydrogenase Reaction, row WT, substrate butyraldehyde, columns Km (mM), kcat (s^-1), and kcat/Km (M^-1 s^-1): 31.7 +/- 2.2; 9.5 +/- 0.2; 300 +/- 20.

normalized_value_unit_evidence: Km 31.7 mM; kcat 9.5 s^-1; printed kcat/Km 0.3 s^-1 mM^-1.

raw_value_unit_evidence: Table 2 WT — butyraldehyde: Km 31.7 +/- 2.2 mM; kcat 9.5 +/- 0.2 s^-1; printed kcat/Km 300 +/- 20 M^-1 s^-1.

row_label: WT — butyraldehyde


substrate_name: pentaldehyde

smiles: CCCCC=O

column_label: Official ACS publisher PDF, Table 2. Substrate Specificity of BphJ in the Dehydrogenase Reaction, row I195A, substrate pentaldehyde, columns Km (mM), kcat (s^-1), and kcat/Km (M^-1 s^-1): 8.2 +/- 0.8; 13.0 +/- 0.4; 1584 +/- 156.

normalized_value_unit_evidence: Km 8.2 mM; kcat 13 s^-1; printed kcat/Km 1.584 s^-1 mM^-1.

row_label: I195A — pentaldehyde

substrate: pentaldehyde


substrate_name: picolinaldehyde

sequence_variant_note: Resolved canonical sequence from uniprotkb_reviewed:Q79AF6 and applied mutation signature I171A to reconstruct the assayed variant sequence.

column_label: Official ACS publisher PDF, Table 2. Substrate Specificity of BphJ in the Dehydrogenase Reaction, row I171A, substrate picolinaldehyde, columns Km (mM), kcat (s^-1), and kcat/Km (M^-1 s^-1): 3.2 +/- 0.3; 1.9 +/- 0.1; 597 +/- 53.

normalized_value_unit_evidence: Km 3.2 mM; kcat 1.9 s^-1; printed kcat/Km 0.597 s^-1 mM^-1.

raw_value_unit_evidence: Table 2 I171A — picolinaldehyde: Km 3.2 +/- 0.3 mM; kcat 1.9 +/- 0.1 s^-1; printed kcat/Km 597 +/- 53 M^-1 s^-1.

row_label: I171A — picolinaldehyde

substrate: picolinaldehyde


sequence_variant_note: Resolved canonical sequence from uniprotkb_reviewed:Q79AF6 and applied mutation signature I171F to reconstruct the assayed variant sequence.

column_label: Official ACS publisher PDF, Table 2. Substrate Specificity of BphJ in the Dehydrogenase Reaction, row I171F, substrate picolinaldehyde, columns Km (mM), kcat (s^-1), and kcat/Km (M^-1 s^-1): 12.0 +/- 1.0; 2.4 +/- 0.1; 202 +/- 17.

normalized_value_unit_evidence: Km 12 mM; kcat 2.4 s^-1; printed kcat/Km 0.202 s^-1 mM^-1.

raw_value_unit_evidence: Table 2 I171F — picolinaldehyde: Km 12.0 +/- 1.0 mM; kcat 2.4 +/- 0.1 s^-1; printed kcat/Km 202 +/- 17 M^-1 s^-1.

row_label: I171F — picolinaldehyde


column_label: Preserved review/source evidence states the PMID 22574886 Table 2 I195A picolinaldehyde entry matches Km 6.9 mM, kcat 3.2 s^-1, and kcat/Km 0.468 mM^-1 s^-1.

normalized_value_unit_evidence: kcat normalized_value=3.2 normalized_unit=s^-1; Km normalized_value=6.9 normalized_unit=mM; kcat/Km normalized_value=0.468 normalized_unit=s^-1 mM^-1

raw_value_unit_evidence: kcat raw_value=3.2 raw_unit=s^-1; Km raw_value=6.9 raw_unit=mM; kcat/Km raw_value=0.468 raw_unit=s^-1 mM^-1

row_label: BphJ I195A / picolinaldehyde

table_label: Preserved PMID 22574886 Table 2 evidence from current verified_catlog note/source record


organism: Corynebacterium glutamicum

substrate_name: NADP+

sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature L36T to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 8; 298.15 K; cofactor NADP+

column_label: Table 2 row L36T / NADP gives Km 1.46 mM, Kcat 95 min^-1, and Kcat/Km 65 mM^-1 min^-1.

normalized_value_unit_evidence: Km normalized_value=1.46 mM; Kcat normalized_value=1.5833333333333333 s^-1; Kcat/Km normalized_value=1.0833333333333333 s^-1 mM^-1

raw_value_unit_evidence: NADP row: Km raw_value=1.46 mM; Kcat raw_value=95 min^-1; Kcat/Km raw_value=65 mM^-1 min^-1

row_label: L36T GAPDH mutant / NADP coenzyme

table_label: ScienceDirect full-text Table 2: Enzyme kinetics of GAPDH and mutants

substrate: NADP+


organism: Crocosphaera subtropica

verification_status: unverified

assay_conditions_summary: pH 9; 298.15 K; cofactor NADP+

status: unverified


substrate_name: NAD+

kcat_over_km_origin: calculated

sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature K154A to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 9; 298.15 K; cofactor NAD+

substrate: NAD+


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature S157E to reconstruct the assayed variant sequence.


substrate_name: succinate semialdehyde

verification_status: verified

smiles: O=CCCC(=O)O

column_label: PLOS ONE DOI 10.1371/journal.pone.0239372 Table 1; Lys154Ala column; NAD+ block SSA rows KmSSA, kcatSSA, and kcat/KmSSA.

raw_value_unit_evidence: Table 1 Lys154Ala NAD+ block for SSA: KmSSA 0.02 +/- 0.01 mM; kcatSSA 0.13 +/- 0.07 s^-1; kcat/KmSSA 6.5 mM^-1 s^-1.

row_label: Lys154Ala / succinate semialdehyde in NAD+ block

table_label: Table 1. Kinetic parameters of cce4228 wild type and variants

substrate: succinate semialdehyde

status: verified


column_label: PLOS ONE DOI 10.1371/journal.pone.0239372 Table 1; Ser157Glu column; NADP+ block SSA rows KmSSA, kcatSSA, and kcat/KmSSA.

raw_value_unit_evidence: Table 1 Ser157Glu NADP+ block for SSA: KmSSA 0.0028 +/- 0.0007 mM; kcatSSA 1.5 +/- 0.1 s^-1; kcat/KmSSA 535 mM^-1 s^-1.

row_label: Ser157Glu / succinate semialdehyde in NADP+ block


column_label: PLOS ONE DOI 10.1371/journal.pone.0239372 Table 1; WT column; NADP+ block SSA rows KmSSA, kcatSSA, and kcat/KmSSA.

raw_value_unit_evidence: Table 1 WT NADP+ block for SSA: KmSSA 0.008 +/- 0.003 mM; kcatSSA 3.9 +/- 0.3 s^-1; kcat/KmSSA 487 mM^-1 s^-1.

row_label: WT / succinate semialdehyde in NADP+ block


enzyme_display_name: Aspartate-semialdehyde dehydrogenase 2

organism: Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

substrate_name: L-aspartate 4-semialdehyde

source_db: uniprot

kcat_display: Not reported

kcat_over_km_display: Not reported

enzyme: Aspartate-semialdehyde dehydrogenase 2

substrate: L-aspartate 4-semialdehyde

source: uniprot


substrate_name: NADP(+)

substrate: NADP(+)


substrate_name: phosphate

substrate: phosphate


enzyme_display_name: Aspartate-semialdehyde dehydrogenase 1

enzyme: Aspartate-semialdehyde dehydrogenase 1


enzyme_display_name: aspartate-semialdehyde dehydrogenase

organism: Vibrio cholerae

substrate_name: L-Aspartate 4-semialdehyde

assay_conditions_summary: pH 9; 303.15 K; cofactor NADP+

column_label: Table 2, Kinetic Parameters of Purified ASA Dehydrogenases; row V. cholerae II; kcat column = 58 s^-1; ASA Km (mM) column = 0.16; ASA kcat/Km (M^-1 s^-1) column = 3.6 x 10^5 (normalized 360 s^-1 mM^-1).

row_label: V. cholerae II / ASA

enzyme: aspartate-semialdehyde dehydrogenase

substrate: L-Aspartate 4-semialdehyde


column_label: Table 2, Kinetic Parameters of Purified ASA Dehydrogenases; row V. cholerae I; kcat column = 120 s^-1; ASA Km (mM) column = 0.19; ASA kcat/Km (M^-1 s^-1) column = 6.3 x 10^5 (normalized 630 s^-1 mM^-1).

row_label: V. cholerae I / ASA


column_label: Table 2, Kinetic Parameters of Purified ASA Dehydrogenases; row V. cholerae II; kcat column = 58 s^-1; NADP Km (mM) column = 0.36; NADP kcat/Km (M^-1 s^-1) column = 1.6 x 10^5 (normalized 160 s^-1 mM^-1).

raw_value_unit_evidence: kcat 58 s^-1; NADP Km 0.36 mM; NADP kcat/Km 1.6 x 10^5 M^-1 s^-1

row_label: V. cholerae II / NADP


column_label: Table 2, Kinetic Parameters of Purified ASA Dehydrogenases; row V. cholerae I; kcat column = 120 s^-1; NADP Km (mM) column = 0.32; NADP kcat/Km (M^-1 s^-1) column = 3.8 x 10^5 (normalized 380 s^-1 mM^-1).

raw_value_unit_evidence: kcat 120 s^-1; NADP Km 0.32 mM; NADP kcat/Km 3.8 x 10^5 M^-1 s^-1

row_label: V. cholerae I / NADP


substrate_name: Phosphate

smiles: O=P(O)(O)O

substrate: Phosphate


column_label: Table 2, Kinetic Parameters of Purified ASA Dehydrogenases; row V. cholerae I; kcat column = 120 s^-1; Phosphate Km (mM) column = 1.1; Phosphate kcat/Km (M^-1 s^-1) column = 1.1 x 10^5 (normalized 110 s^-1 mM^-1).

raw_value_unit_evidence: kcat 120 s^-1; Phosphate Km 1.1 mM; Phosphate kcat/Km 1.1 x 10^5 M^-1 s^-1

row_label: V. cholerae I / Phosphate


enzyme_display_name: Aldehyde-alcohol dehydrogenase 2

organism: Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM)

substrate_name: ethanol

smiles: CCO

assay_conditions_summary: cofactor NAD+

column_label: Km values for acetyl-CoA, acetaldehyde and ethanol were 0.015, 0.15 and 80 mM respectively in the presence of 0.2 mM NAD+.

raw_value_unit_evidence: Km raw_value=80 mM raw_unit=mM

row_label: Prose segment: ethanol Km

table_label: PubMed PMID 7980441 abstract

enzyme: Aldehyde-alcohol dehydrogenase 2

substrate: ethanol


raw_value_unit_evidence: Km raw_value=0.15 mM raw_unit=mM

row_label: Prose segment: acetaldehyde Km


substrate_name: acetyl-CoA

assay_conditions_summary: pH 7.5; cofactor NAD+

column_label: PMCID PMC1137609 scanned page 745, left column, Molecular characterization of EhADH2, K_m values paragraph; acetyl-CoA is listed in the ACDH activity sentence.

raw_value_unit_evidence: Primary paper page 745 reports ACDH K_m 0.015 mM for acetyl-CoA.

row_label: ACDH activity; acetyl-CoA

table_label: Prose: Molecular characterization of EhADH2 / K_m values

substrate: acetyl-CoA


substrate_name: NAD(+) (for ADH activity)

substrate: NAD(+) (for ADH activity)


substrate_name: NADH (for ADH activity)

assay_conditions_summary: cofactor NADH

substrate: NADH (for ADH activity)


enzyme_display_name: Aspartate-semialdehyde dehydrogenase

organism: Escherichia coli (strain K12)

substrate_name: L-4-aspartyl phosphate

column_label: Table 1, Enzyme ASD, Km column, ASD / aspp row: 0.022 mM.

normalized_value_unit_evidence: 0.022 mM; already in mM in Table 1.

raw_value_unit_evidence: Table 1 lists ASD aspp Km = 0.022 +/- 0.001 mM; aspp is beta-aspartyl phosphate.

row_label: ASD / aspp row

table_label: Table 1: Kinetic parameters of the threonine-pathway enzymes

enzyme: Aspartate-semialdehyde dehydrogenase

substrate: L-4-aspartyl phosphate


substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

column_label: Table 1, Enzyme ASD, Km column, ASD / NADPH row: 0.029 mM.

normalized_value_unit_evidence: 0.029 mM; already in mM in Table 1.

raw_value_unit_evidence: Table 1 lists ASD NADPH Km = 0.029 +/- 0.002 mM.

row_label: ASD / NADPH row

substrate: NADPH


column_label: Table 1, Enzyme ASD, Km column, ASD / ASA row: 0.11 mM.

normalized_value_unit_evidence: 0.11 mM; already in mM in Table 1.

raw_value_unit_evidence: Table 1 lists ASD ASA Km = 0.11 +/- 0.008 mM; ASA is D,L-aspartic beta-semialdehyde.

row_label: ASD / ASA row


assay_conditions_summary: cofactor NADP+

column_label: Table 1, Enzyme ASD, Km column, ASD / NADP+ row: 0.144 mM.

normalized_value_unit_evidence: 0.144 mM; already in mM in Table 1.

raw_value_unit_evidence: Table 1 lists ASD NADP+ Km = 0.144 +/- 0.02 mM.

row_label: ASD / NADP+ row


column_label: Table 1, Enzyme ASD, Km column, ASD / Pi row: 10.2 mM.

normalized_value_unit_evidence: 10.2 mM; already in mM in Table 1.

raw_value_unit_evidence: Table 1 lists ASD Pi Km = 10.2 +/- 1.4 mM.

row_label: ASD / Pi row


enzyme_display_name: UniProt P44801

organism: Haemophilus influenzae

source_db: skid

source_license: CC BY 4.0; CC BY-NC-ND 4.0

column_label: Table 2, Kinetic Parameters of Purified ASA Dehydrogenases; row Ha. influenzae; kcat column = 330 s^-1; Phosphate Km (mM) column = 1.6; Phosphate kcat/Km (M^-1 s^-1) column = 2.1 x 10^5 (normalized 210 s^-1 mM^-1).

raw_value_unit_evidence: kcat 330 s^-1; Phosphate Km 1.6 mM; Phosphate kcat/Km 2.1 x 10^5 M^-1 s^-1

row_label: Ha. influenzae / Phosphate

source_databases_merged: skid uniprot

enzyme: UniProt P44801

source: skid


column_label: Table 2, Kinetic Parameters of Purified ASA Dehydrogenases; row Ha. influenzae; kcat column = 330 s^-1; ASA Km (mM) column = 0.24; ASA kcat/Km (M^-1 s^-1) column = 1.4 x 10^6 (normalized 1400 s^-1 mM^-1).

row_label: Ha. influenzae / ASA


column_label: Table 2, Kinetic Parameters of Purified ASA Dehydrogenases; row Ha. influenzae; kcat column = 330 s^-1; NADP Km (mM) column = 0.15; NADP kcat/Km (M^-1 s^-1) column = 2.2 x 10^6 (normalized 2200 s^-1 mM^-1).

raw_value_unit_evidence: kcat 330 s^-1; NADP Km 0.15 mM; NADP kcat/Km 2.2 x 10^6 M^-1 s^-1

row_label: Ha. influenzae / NADP


table_label: Table 2, Kinetic Parameters of Purified ASA Dehydrogenases


organism: Haloarcula vallismortis

substrate_name: D-glyceraldehyde 3-phosphate

source_db: brenda

sequence: KVLISAPPKGDEPVKKILTWYDNEYGFSNRMLDVAEYIT

wild_type_sequence: KVLISAPPKGDEPVKKILTWYDNEYGFSNRMLDVAEYIT

smiles: C(C(C=O)O)OP(=O)(O)O

assay_conditions_summary: pH 7.8; cofactor NAD+

substrate: D-glyceraldehyde 3-phosphate

source: brenda


substrate_name: arsenate

substrate: arsenate


organism: Escherichia coli

sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature Q350N with inferred residue-numbering offset +15 to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 9; 303.15 K; cofactor NAD+


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature Q350N/H171A to reconstruct the assayed variant sequence.


enzyme_display_name: UniProt P9WNX5

organism: Mycobacterium tuberculosis

assay_conditions_summary: pH 9; 303.15 K; cofactor NADPH

column_label: OUP full text Table 1 row Pi reports Km (mM)=11.39.

raw_value_unit_evidence: Table 1 row Pi reports Km raw_value=11.39 raw_unit=mM.

row_label: Pi

table_label: Table 1: Apparent kinetic parameters of purified recombinant ASD of Mycobacterium tuberculosis H37Rv

enzyme: UniProt P9WNX5


column_label: Primary OUP full-text Table 1 row L-ASA; columns Km (mM) and Kcat/Km (M^-1 s^-1); exact printed cells 0.955 and 8.89 x 10^3, respectively.

raw_value_unit_evidence: Table 1 row L-ASA reports Km=0.955 mM and an explicit kcat/Km=8.89 x 10^3 M^-1 s^-1; the article reports turnover number kcat=8.49 s^-1.

row_label: L-ASA


column_label: Primary OUP full-text Table 1 row NADP; columns Km (mM) and Kcat/Km (M^-1 s^-1); exact printed cells 0.065 and 1.31 x 10^5, respectively.

raw_value_unit_evidence: Table 1 row NADP reports Km=0.065 mM and an explicit kcat/Km=1.31 x 10^5 M^-1 s^-1; the article reports turnover number kcat=8.49 s^-1.

row_label: NADP


column_label: Primary OUP full-text Table 1 row Pi; columns Km (mM) and Kcat/Km (M^-1 s^-1); exact printed cells 11.39 and 7.45 x 10^2, respectively.

raw_value_unit_evidence: Table 1 row Pi reports Km=11.39 mM and an explicit kcat/Km=7.45 x 10^2 M^-1 s^-1; the article reports turnover number kcat=8.49 s^-1.


enzyme_display_name: UniProt B2HN69

organism: Mycobacterium marinum

source_license: CC BY-NC-ND 4.0

enzyme: UniProt B2HN69


substrate_name: ATP

substrate: ATP


substrate_name: benzoic acid

substrate: benzoic acid


organism: Candida maltosa


substrate_name: alpha-aminoadipate

smiles: C(CC(C(=O)O)N)CC(=O)O

substrate: alpha-aminoadipate


substrate_name: NADH

substrate: NADH


organism: Acanthopagrus schlegelii

substrate_name: acetaldehyde

sequence: SGLLINQPKYSWLKELGL

wild_type_sequence: SGLLINQPKYSWLKELGL

substrate: acetaldehyde


substrate_name: D-Glyceraldehyde 3-phosphate

substrate: D-Glyceraldehyde 3-phosphate


sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset +10, so it is treated as a source-provided variant sequence.

proof_lines: Two single [Gly317] and [Ala317] GAPDH mutants... were constructed.


substrate_name: NAD+

sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset +3, so it is treated as a source-provided variant sequence.

assay_conditions_summary: pH 8.9; 295.15 K; cofactor NAD+

proof_lines: the role of the invariant Asn 313 residue... was examined.

substrate: NAD+


sequence_variant_note: Mutation signature Y317G matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: dissociation constant for NAD+ is 13 times higher


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature N313T/Y317G with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.

proof_lines: dissociation constant for NAD+ is 300 times higher


organism: Geobacillus stearothermophilus

sequence_variant_note: Mutation signature D32A/L187N could not be applied safely because the resolved sequence does not match the expected reference residue(s).

assay_conditions_summary: pH 9.2; 298.15 K; cofactor NADP+


sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset -4, so it is treated as a source-provided variant sequence.

assay_conditions_summary: pH 9.2; 298.15 K; cofactor NAD+


substrate_name: NADP+

substrate: NADP+


column_label: Table II row Wild-type B. stearothermophilus GAPDH / D-Glyceraldehyde 3-phosphate (GAPDH activity): kcat cell 76 s^-1; KM cell 0.9 mM; no kcat/KM column/cell is printed, so kcat_over_km 84.44444444444444 s^-1 mM^-1 is the quotient 76 / 0.9 of the two printed cells.

normalized_value_unit_evidence: kcat 76 s^-1; Km 0.9 mM; kcat/Km derived as 76 / 0.9 = 84.44444444444444 s^-1 mM^-1 from the two printed component cells; the paper prints no kcat/KM value.

raw_value_unit_evidence: Table II row Wild-type B. stearothermophilus GAPDH / D-Glyceraldehyde 3-phosphate (GAPDH activity): kcat=76 s^-1; km=0.9 mM; kcat_over_km=not explicitly reported.

row_label: Wild-type B. stearothermophilus GAPDH | D-Glyceraldehyde 3-phosphate (GAPDH activity)

table_label: Table II. Kinetic parameters of wild-type and mutants of GapB-encoded protein, and wild-type and mutant of B. stearothermophilus GAPDH


substrate_name: Glycerate 1,3-bisphosphate

assay_conditions_summary: pH 7; 298.15 K; buffer pH 7 for the reverse reaction; cofactor NADH

column_label: Results section, reverse-direction paragraph of JBC 1997, 272(24):15106-15112 (reverse reaction with 1,3-dPG); Results prose, reverse direction with 1,3-dPG, GAPDH of B. stearothermophilus; the kcat value 65 s-1 comes from the reverse-reaction turnover number stated in the Results prose and the Michaelis constant 0.005 mM comes from the reverse-reaction Michaelis constant for 1,3-dPG stated in the Results prose. There is no kcat/Km column.

normalized_value_unit_evidence: kcat: printed cell 65 s-1; already in s^-1, no conversion (this article prints no min^-1 kinetic value); the paper value is 65.0 s^(-1). Km: printed cell 0.005 mM; already in mM, no conversion; the paper value is 0.005 mM. kcat/Km: this article prints no kcat/Km cell for this measurement, so the exported 13000.0 s^(-1)*mM^(-1) is not a printed value; it is the quotient 65 / 0.005 of this measurement's own two printed values, typed derived_same_measurement.

raw_value_unit_evidence: Results prose, reverse direction with 1,3-dPG, GAPDH of B. stearothermophilus: kcat = 65 s-1 (printed column the reverse-reaction turnover number stated in the Results prose); Km = 0.005 mM (printed column the reverse-reaction Michaelis constant for 1,3-dPG stated in the Results prose). No kcat/Km value is printed for this measurement.

substrate: Glycerate 1,3-bisphosphate


assay_conditions_summary: pH 8.9; 298.15 K; cofactor NAD+

column_label: Table II row Wild-type B. stearothermophilus GAPDH / NAD+ (GAPDH activity): kcat cell 76 s^-1; KM cell 0.09 mM; no kcat/KM column/cell is printed, so kcat_over_km 844.4444444444445 s^-1 mM^-1 is the quotient 76 / 0.09 of the two printed cells.

normalized_value_unit_evidence: kcat 76 s^-1; Km 0.09 mM; kcat/Km derived as 76 / 0.09 = 844.4444444444445 s^-1 mM^-1 from the two printed component cells; the paper prints no kcat/KM value.

raw_value_unit_evidence: Table II row Wild-type B. stearothermophilus GAPDH / NAD+ (GAPDH activity): kcat=76 s^-1; km=0.09 mM; kcat_over_km=not explicitly reported.

row_label: Wild-type B. stearothermophilus GAPDH | NAD+ (GAPDH activity)


column_label: Results main text, JBC page 15110, paragraph on the reverse direction with 1,3-dPG as substrate; Wild-type B. stearothermophilus GAPDH / NADH (reverse reaction with 1,3-dPG): kcat 65 s^-1; KM 0.011 mM; no kcat/KM value is printed, so kcat_over_km 5909.090909090909 s^-1 mM^-1 is the quotient 65 / 0.011 of the two printed prose values.

normalized_value_unit_evidence: kcat 65 s^-1; Km 0.011 mM; kcat/Km derived as 65 / 0.011 = 5909.090909090909 s^-1 mM^-1 from the two printed prose values; the paper prints no kcat/KM value.

raw_value_unit_evidence: Results main text, JBC page 15110, paragraph on the reverse direction with 1,3-dPG as substrate; Wild-type B. stearothermophilus GAPDH / NADH (reverse reaction with 1,3-dPG): kcat=65 s^-1; km=0.011 mM; kcat_over_km=5909.090909090909 s^-1 mM^-1 derived as the quotient 65 / 0.011 s^-1 / mM; no kcat/KM value is printed in the paper.


enzyme_display_name: Glyceraldehyde-3-phosphate dehydrogenase 1, cytosolic

organism: Oryza sativa subsp. japonica


substrate_name: NAD(+)

enzyme: Glyceraldehyde-3-phosphate dehydrogenase 1, cytosolic

substrate: NAD(+)


substrate_name: 3-phospho-D-glyceroyl phosphate

substrate: 3-phospho-D-glyceroyl phosphate


organism: Saccharomyces cerevisiae

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

assay_conditions_summary: pH 8; cofactor NADPH

column_label: Kinetic constants paragraph; TPNH row/term; K = 8.3 x 10^-5 M.

raw_value_unit_evidence: Kinetic constants paragraph reports K for TPNH 8.3 x 10^-5 M; summary repeats TPNH 8.3 x 10^-5.

row_label: TPNH

table_label: Kinetic constants paragraph and summary for aspartic beta-semialdehyde dehydrogenase


column_label: Kinetic constants paragraph; BAP row/term; K = 1.6 x 10^-4 M.

raw_value_unit_evidence: Kinetic constants paragraph reports K for BAP 1.6 x 10^-4 M; summary repeats BAP 1.6 x 10^-4.

row_label: BAP


substrate_name: L-aspartate 4-semialdehyde

assay_conditions_summary: pH 9; cofactor NADPH

column_label: Kinetic constants paragraph; ASA row/term; K = 2.6 x 10^-3 M.

raw_value_unit_evidence: Kinetic constants paragraph reports K values for ASA, TPN, and phosphate are respectively 2.6 x 10^-3 M, 3.6 x 10^-5 M, and 1.4 x 10^-3 M; summary repeats ASA 2.6 x 10^-3.

row_label: ASA

substrate: L-aspartate 4-semialdehyde


substrate_name: 3-Phospho-D-glyceroyl phosphate

substrate: 3-Phospho-D-glyceroyl phosphate


assay_conditions_summary: pH 6.5; 303.15 K; cofactor NADPH

column_label: Table 2; reaction TDH; isoenzyme Tdh2p; parameter kcat value 8.63 s^-1; parameter Kgap value 0.77 mM; kcat/Km computed from those explicit cells

normalized_value_unit_evidence: kcat normalized_value=8.63 normalized_unit=s^-1; Km normalized_value=0.77 normalized_unit=mM; kcat/Km normalized_value=11.207792207792208 normalized_unit=s^-1*mM^-1

raw_value_unit_evidence: kcat raw_value=8.63 raw_unit=s^-1; Km raw_value=0.77 raw_unit=mM; kcat/Km computed from explicit Table 2 cells 8.63/0.77 raw_unit=s^-1*mM^-1

row_label: TDH / Tdh2p / Kgap

table_label: Table 2. Enzyme kinetics parameters determined in this project and contrasted with those of [23].


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature S22Q/P24K to reconstruct the assayed variant sequence.

column_label: Table 2; reaction TDH; isoenzyme Tdh3p; parameter kcat value 18.2 s^-1; parameter Kgap value 0.423 mM; kcat/Km computed from those explicit cells

normalized_value_unit_evidence: kcat normalized_value=18.2 normalized_unit=s^-1; Km normalized_value=0.423 normalized_unit=mM; kcat/Km normalized_value=43.026004728132385 normalized_unit=s^-1*mM^-1

raw_value_unit_evidence: kcat raw_value=18.2 raw_unit=s^-1; Km raw_value=0.423 raw_unit=mM; kcat/Km computed from explicit Table 2 cells 18.2/0.423 raw_unit=s^-1*mM^-1

row_label: TDH / Tdh3p / Kgap


column_label: Table 2; reaction TDH; isoenzyme Tdh1p; parameter kcat value 19.1 s^-1; parameter Kgap value 0.495 mM; kcat/Km computed from those explicit cells

normalized_value_unit_evidence: kcat normalized_value=19.1 normalized_unit=s^-1; Km normalized_value=0.495 normalized_unit=mM; kcat/Km normalized_value=38.58585858585859 normalized_unit=s^-1*mM^-1

raw_value_unit_evidence: kcat raw_value=19.1 raw_unit=s^-1; Km raw_value=0.495 raw_unit=mM; kcat/Km computed from explicit Table 2 cells 19.1/0.495 raw_unit=s^-1*mM^-1

row_label: TDH / Tdh1p / Kgap


enzyme_display_name: carboxylate reductase (NADP+)

organism: Tsukamurella paurometabola

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

enzyme: carboxylate reductase (NADP+)


substrate_name: octadecanoate

smiles: CCCCCCCCCCCCCCCCCC(=O)[O-]

substrate: octadecanoate


substrate_name: Butanoate

smiles: CCCC(=O)[O-]

substrate: Butanoate


organism: Homo sapiens

substrate_name: Coenzyme A; alpha-Ketovaline; NAD+

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

substrate: Coenzyme A; alpha-Ketovaline; NAD+


substrate_name: alpha-Ketovaline; Coenzyme A; NAD+

substrate: alpha-Ketovaline; Coenzyme A; NAD+


enzyme_display_name: branched-chain alpha-keto acid dehydrogenase system

substrate_name: alpha-Ketovaline

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

smiles: CC(C)C(=O)C(=O)O

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NAD+

substrate: alpha-Ketovaline


enzyme: branched-chain alpha-keto acid dehydrogenase system


substrate_name: thiamine diphosphate

substrate: thiamine diphosphate


substrate_name: L-2-Aminoadipate; H+; NADPH

substrate: L-2-Aminoadipate; H+; NADPH


substrate_name: NADPH

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NADPH

substrate: NADPH


enzyme_display_name: benzaldehyde dehydrogenase (NAD+)

organism: Acinetobacter calcoaceticus

enzyme: benzaldehyde dehydrogenase (NAD+)


substrate_name: benzaldehyde

substrate: benzaldehyde


organism: Pseudomonas aeruginosa

substrate_name: L-Aspartate 4-semialdehyde

column_label: Table 2, Kinetic Parameters of Purified ASA Dehydrogenases; row P. aeruginosa; kcat column = 160 s^-1; ASA Km (mM) column = 0.12; ASA kcat/Km (M^-1 s^-1) column = 1.3 x 10^6 (normalized 1300 s^-1 mM^-1).

row_label: P. aeruginosa / ASA

substrate: L-Aspartate 4-semialdehyde


assay_conditions_summary: pH 9; 303.15 K; cofactor NADP+

column_label: Table 2, Kinetic Parameters of Purified ASA Dehydrogenases; row P. aeruginosa; kcat column = 160 s^-1; NADP Km (mM) column = 0.13; NADP kcat/Km (M^-1 s^-1) column = 1.6 x 10^6 (normalized 1600 s^-1 mM^-1).

raw_value_unit_evidence: kcat 160 s^-1; NADP Km 0.13 mM; NADP kcat/Km 1.6 x 10^6 M^-1 s^-1

row_label: P. aeruginosa / NADP


substrate_name: Phosphate

column_label: Table 2, Kinetic Parameters of Purified ASA Dehydrogenases; row P. aeruginosa; kcat column = 160 s^-1; Phosphate Km (mM) column = 1.5; Phosphate kcat/Km (M^-1 s^-1) column = 1.1 x 10^5 (normalized 110 s^-1 mM^-1).

raw_value_unit_evidence: kcat 160 s^-1; Phosphate Km 1.5 mM; Phosphate kcat/Km 1.1 x 10^5 M^-1 s^-1

row_label: P. aeruginosa / Phosphate

substrate: Phosphate


substrate_name: perillaldehyde

substrate: perillaldehyde


substrate_name: cinnamaldehyde

substrate: cinnamaldehyde


smiles: CCCCCCCC=O


smiles: CCCCCC=O


km_display: Not reported


assay_conditions_summary: pH 7.8; cofactor NADPH

column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups row tpCAR (Tsukamurella paurometabola), substrate column 4-Nitrobenzoic acid (6), metric row k cat [min^-1], raw cell 13+/-1, normalized 0.216666666666666666666666666667 s^-1; Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups row tpCAR (Tsukamurella paurometabola), substrate column 4-Nitrobenzoic acid (6), metric row k cat/K_m [min^-1 mM^-1], raw cell 22+/-6, normalized 0.366666666666666666666666666667 s^-1*mM^-1; Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups row tpCAR (Tsukamurella paurometabola), substrate column 4-Nitrobenzoic acid (6), metric row K_m [mM], raw cell 0.6+/-0.1, normalized 0.6 mM

normalized_value_unit_evidence: kcat normalized_value=0.216666666666666666666666666667 normalized_unit=s^-1; kcat/Km normalized_value=0.366666666666666666666666666667 normalized_unit=s^-1*mM^-1; Km normalized_value=0.6 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=13 raw_unit=min^-1 raw_cell=13+/-1; kcat/Km raw_value=22 raw_unit=min^-1*mM^-1 raw_cell=22+/-6; Km raw_value=0.6 raw_unit=mM raw_cell=0.6+/-0.1

row_label: tpCAR (Tsukamurella paurometabola) / 4-Nitrobenzoic acid (6)


substrate_name: benzoate

column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row tpCAR (Tsukamurella paurometabola) / Benzoic acid (1); kcat column kcat [min^-1] raw 142+/-3; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 72+/-6; Km column Km [mM] raw 2.0+/-0.1

normalized_value_unit_evidence: kcat normalized_value=2.37 normalized_unit=s^-1 from 142+/-3 / 60; kcat/Km normalized_value=1.185 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=72+/-6 min^-1 mM^-1; Km normalized_value=2.0 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=142+/-3 raw_unit=min^-1; kcat/Km raw_value=72+/-6 raw_unit=min^-1 mM^-1; Km raw_value=2.0+/-0.1 raw_unit=mM

substrate: benzoate


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row tpCAR (Tsukamurella paurometabola) / 4-Methylbenzoic acid (2); kcat column kcat [min^-1] raw 152+/-2; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 220+/-10; Km column Km [mM] raw 0.69+/-0.03

normalized_value_unit_evidence: kcat normalized_value=2.53 normalized_unit=s^-1 from 152+/-2 / 60; kcat/Km normalized_value=3.6666666666666665 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=220+/-10 min^-1 mM^-1; Km normalized_value=0.69 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=152+/-2 raw_unit=min^-1; kcat/Km raw_value=220+/-10 raw_unit=min^-1 mM^-1; Km raw_value=0.69+/-0.03 raw_unit=mM


substrate_name: dodecanoate

smiles: CCCCCCCCCCCC(=O)[O-]

column_label: Table 4. CAR activity against heterocycles and fatty acids.; row tpCAR (Tsukamurella paurometabola) / Dodecanoic acid (20); kcat column kcat [min^-1] raw 157+/-5; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 3600+/-400; Km column Km [mM] raw 0.04+/-0.01

normalized_value_unit_evidence: kcat normalized_value=2.62 normalized_unit=s^-1 from 157+/-5 / 60; kcat/Km normalized_value=65.5 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=3600+/-400 min^-1 mM^-1; Km normalized_value=0.04 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=157+/-5 raw_unit=min^-1; kcat/Km raw_value=3600+/-400 raw_unit=min^-1 mM^-1; Km raw_value=0.04+/-0.01 raw_unit=mM

substrate: dodecanoate


column_label: Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring.; row tpCAR (Tsukamurella paurometabola) / 3-Phenylpropionic acid (9); kcat column kcat [min^-1] raw 158+/-2; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 500+/-20; Km column Km [mM] raw 0.32+/-0.01

normalized_value_unit_evidence: kcat normalized_value=2.63 normalized_unit=s^-1 from 158+/-2 / 60; kcat/Km normalized_value=8.21875 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=500+/-20 min^-1 mM^-1; Km normalized_value=0.32 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=158+/-2 raw_unit=min^-1; kcat/Km raw_value=500+/-20 raw_unit=min^-1 mM^-1; Km raw_value=0.32+/-0.01 raw_unit=mM


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row tpCAR (Tsukamurella paurometabola) / 3-Methoxybenzoic acid (4); kcat column kcat [min^-1] raw 186+/-2; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 334+/-10; Km column Km [mM] raw 0.56+/-0.02

normalized_value_unit_evidence: kcat normalized_value=3.1 normalized_unit=s^-1 from 186+/-2 / 60; kcat/Km normalized_value=5.535714285714286 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=334+/-10 min^-1 mM^-1; Km normalized_value=0.56 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=186+/-2 raw_unit=min^-1; kcat/Km raw_value=334+/-10 raw_unit=min^-1 mM^-1; Km raw_value=0.56+/-0.02 raw_unit=mM


substrate_name: Octanoate

smiles: CCCCCCCC(=O)[O-]

column_label: Table 4. CAR activity against heterocycles and fatty acids.; row tpCAR (Tsukamurella paurometabola) / Octanoic acid (19); kcat column kcat [min^-1] raw 219+/-3; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 1140+/-50; Km column Km [mM] raw 0.2+/-0.01

normalized_value_unit_evidence: kcat normalized_value=3.65 normalized_unit=s^-1 from 219+/-3 / 60; kcat/Km normalized_value=18.25 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=1140+/-50 min^-1 mM^-1; Km normalized_value=0.2 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=219+/-3 raw_unit=min^-1; kcat/Km raw_value=1140+/-50 raw_unit=min^-1 mM^-1; Km raw_value=0.2+/-0.01 raw_unit=mM

substrate: Octanoate


organism: Nocardia otitidiscaviarum

assay_conditions_summary: pH 7.5; cofactor NADPH

column_label: Table 4. CAR activity against heterocycles and fatty acids.; row noCAR (Nocardia otitidiscaviarum) / Octanoic acid (19); kcat column kcat [min^-1] raw 141+/-2; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 750+/-30; Km column Km [mM] raw 0.2+/-0.01

normalized_value_unit_evidence: kcat normalized_value=2.35 normalized_unit=s^-1 from 141+/-2 / 60; kcat/Km normalized_value=11.75 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=750+/-30 min^-1 mM^-1; Km normalized_value=0.2 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=141+/-2 raw_unit=min^-1; kcat/Km raw_value=750+/-30 raw_unit=min^-1 mM^-1; Km raw_value=0.2+/-0.01 raw_unit=mM


column_label: Table 4. CAR activity against heterocycles and fatty acids.; row noCAR (Nocardia otitidiscaviarum) / Butanoic acid (18); kcat column kcat [min^-1] raw 170+/-20*; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 3.4+/-0.7*; Km column Km [mM] raw 50+/-8*

normalized_value_unit_evidence: kcat normalized_value=2.83 normalized_unit=s^-1 from 170+/-20* / 60; kcat/Km normalized_value=0.056600000000000004 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=3.4+/-0.7* min^-1 mM^-1; Km normalized_value=50.0 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=170+/-20* raw_unit=min^-1; kcat/Km raw_value=3.4+/-0.7* raw_unit=min^-1 mM^-1; Km raw_value=50+/-8* raw_unit=mM


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row noCAR (Nocardia otitidiscaviarum) / Benzoic acid (1); kcat column kcat [min^-1] raw 183+/-6; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 89.1+/-8; Km column Km [mM] raw 2.1+/-0.2

normalized_value_unit_evidence: kcat normalized_value=3.05 normalized_unit=s^-1 from 183+/-6 / 60; kcat/Km normalized_value=1.4523809523809523 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=89.1+/-8 min^-1 mM^-1; Km normalized_value=2.1 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=183+/-6 raw_unit=min^-1; kcat/Km raw_value=89.1+/-8 raw_unit=min^-1 mM^-1; Km raw_value=2.1+/-0.2 raw_unit=mM


organism: Mycolicibacterium phlei


assay_conditions_summary: pH 7.3; 303.15 K; cofactor ATP

proof_lines: pH and temperature not specified in the publication, versus ATP


assay_conditions_summary: pH 7.3; cofactor NADPH

column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row mpCAR (Mycobacterium phlei) / Benzoic acid (1); kcat column kcat [min^-1] raw 140+/-20*; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 7+/-1*; Km column Km [mM] raw 20+/-4*

normalized_value_unit_evidence: kcat normalized_value=2.33 normalized_unit=s^-1 from 140+/-20* / 60; kcat/Km normalized_value=0.1165 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=7+/-1* min^-1 mM^-1; Km normalized_value=20.0 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=140+/-20* raw_unit=min^-1; kcat/Km raw_value=7+/-1* raw_unit=min^-1 mM^-1; Km raw_value=20+/-4* raw_unit=mM


organism: Mycolicibacterium smegmatis

column_label: Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring.; row msCAR (Mycobacterium smegmatis) / 3-Phenylpropionic acid (9); kcat column kcat [min^-1] raw 184+/-9; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 1200+/-200; Km column Km [mM] raw 0.16+/-0.02

normalized_value_unit_evidence: kcat normalized_value=3.07 normalized_unit=s^-1 from 184+/-9 / 60; kcat/Km normalized_value=19.1875 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=1200+/-200 min^-1 mM^-1; Km normalized_value=0.16 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=184+/-9 raw_unit=min^-1; kcat/Km raw_value=1200+/-200 raw_unit=min^-1 mM^-1; Km raw_value=0.16+/-0.02 raw_unit=mM


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row msCAR (Mycobacterium smegmatis) / Benzoic acid (1); kcat column kcat [min^-1] raw 197+/-4; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 57+/-4; Km column Km [mM] raw 3.4+/-0.2

normalized_value_unit_evidence: kcat normalized_value=3.28 normalized_unit=s^-1 from 197+/-4 / 60; kcat/Km normalized_value=0.9647058823529412 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=57+/-4 min^-1 mM^-1; Km normalized_value=3.4 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=197+/-4 raw_unit=min^-1; kcat/Km raw_value=57+/-4 raw_unit=min^-1 mM^-1; Km raw_value=3.4+/-0.2 raw_unit=mM


column_label: Table 4. CAR activity against heterocycles and fatty acids.; row msCAR (Mycobacterium smegmatis) / Octanoic acid (19); kcat column kcat [min^-1] raw 296+/-8; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 3000+/-300; Km column Km [mM] raw 0.1+/-0.01

normalized_value_unit_evidence: kcat normalized_value=4.93 normalized_unit=s^-1 from 296+/-8 / 60; kcat/Km normalized_value=49.3 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=3000+/-300 min^-1 mM^-1; Km normalized_value=0.1 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=296+/-8 raw_unit=min^-1; kcat/Km raw_value=3000+/-300 raw_unit=min^-1 mM^-1; Km raw_value=0.1+/-0.01 raw_unit=mM


organism: Nocadia sp.

assay_conditions_summary: cofactor NADPH


substrate_name: (R)-ibuprofen

substrate: (R)-ibuprofen


substrate_name: ATP

assay_conditions_summary: cofactor ATP

substrate: ATP


substrate_name: Unknown

ki_unit: mM

substrate: Unknown


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row msCAR (Mycobacterium smegmatis) / 3-Methoxybenzoic acid (4); kcat column kcat [min^-1] raw 18+/-1*; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 1.4+/-0.2*; Km column Km [mM] raw 12+/-1*

normalized_value_unit_evidence: kcat normalized_value=0.3 normalized_unit=s^-1 from 18+/-1* / 60; kcat/Km normalized_value=0.024999999999999998 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=1.4+/-0.2* min^-1 mM^-1; Km normalized_value=12.0 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=18+/-1* raw_unit=min^-1; kcat/Km raw_value=1.4+/-0.2* raw_unit=min^-1 mM^-1; Km raw_value=12+/-1* raw_unit=mM


assay_conditions_summary: pH 8; 303.15 K; cofactor NADPH

column_label: Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring row msCAR (Mycolicibacterium smegmatis), substrate column trans-2-Phenylcyclopropane-1-carboxylic acid (13), metric row k cat [min^-1], raw cell 2.2+/-0.1, normalized 0.0366666666666666666666666666667 s^-1; Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring row msCAR (Mycolicibacterium smegmatis), substrate column trans-2-Phenylcyclopropane-1-carboxylic acid (13), metric row K_m [mM], raw cell 0.006+/-0.0001, normalized 0.006 mM

normalized_value_unit_evidence: kcat normalized_value=0.0366666666666666666666666666667 normalized_unit=s^-1; Km normalized_value=0.006 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=2.2 raw_unit=min^-1 raw_cell=2.2+/-0.1; Km raw_value=0.006 raw_unit=mM raw_cell=0.006+/-0.0001

row_label: msCAR (Mycolicibacterium smegmatis) / trans-2-Phenylcyclopropane-1-carboxylic acid (13)


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row msCAR (Mycobacterium smegmatis) / 3-Nitrobenzoic acid (7); kcat column kcat [min^-1] raw 40+/-10; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 100+/-50; Km column Km [mM] raw 0.5+/-0.2

normalized_value_unit_evidence: kcat normalized_value=0.67 normalized_unit=s^-1 from 40+/-10 / 60; kcat/Km normalized_value=1.34 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=100+/-50 min^-1 mM^-1; Km normalized_value=0.5 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=40+/-10 raw_unit=min^-1; kcat/Km raw_value=100+/-50 raw_unit=min^-1 mM^-1; Km raw_value=0.5+/-0.2 raw_unit=mM


substrate_name: octadecanoate

column_label: Table 4. CAR activity against heterocycles and fatty acids.; row msCAR (Mycobacterium smegmatis) / Octadecanoic acid (21); kcat column kcat [min^-1] raw 46+/-4; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 80+/-10; Km column Km [mM] raw 0.6+/-0.09

normalized_value_unit_evidence: kcat normalized_value=0.77 normalized_unit=s^-1 from 46+/-4 / 60; kcat/Km normalized_value=1.2833333333333334 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=80+/-10 min^-1 mM^-1; Km normalized_value=0.6 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=46+/-4 raw_unit=min^-1; kcat/Km raw_value=80+/-10 raw_unit=min^-1 mM^-1; Km raw_value=0.6+/-0.09 raw_unit=mM

substrate: octadecanoate


column_label: Table 4. CAR activity against heterocycles and fatty acids.; row msCAR (Mycobacterium smegmatis) / Furan-2-carboxylic acid (16); kcat column kcat [min^-1] raw 50+/-10; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 4+/-2; Km column Km [mM] raw 13+/-4

normalized_value_unit_evidence: kcat normalized_value=0.83 normalized_unit=s^-1 from 50+/-10 / 60; kcat/Km normalized_value=0.06384615384615384 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=4+/-2 min^-1 mM^-1; Km normalized_value=13.0 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=50+/-10 raw_unit=min^-1; kcat/Km raw_value=4+/-2 raw_unit=min^-1 mM^-1; Km raw_value=13+/-4 raw_unit=mM


column_label: Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring.; row msCAR (Mycobacterium smegmatis) / 3-Oxo-3-phenylpropanoic acid (12); kcat column kcat [min^-1] raw 75+/-2; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 280+/-20; Km column Km [mM] raw 0.27+/-0.02

normalized_value_unit_evidence: kcat normalized_value=1.25 normalized_unit=s^-1 from 75+/-2 / 60; kcat/Km normalized_value=4.62962962962963 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=280+/-20 min^-1 mM^-1; Km normalized_value=0.27 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=75+/-2 raw_unit=min^-1; kcat/Km raw_value=280+/-20 raw_unit=min^-1 mM^-1; Km raw_value=0.27+/-0.02 raw_unit=mM


column_label: Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring.; row msCAR (Mycobacterium smegmatis) / (E)-3-Phenylprop-2-enoic acid (10); kcat column kcat [min^-1] raw 118+/-2; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 1600+/-500; Km column Km [mM] raw 0.075+/-0.004

normalized_value_unit_evidence: kcat normalized_value=1.97 normalized_unit=s^-1 from 118+/-2 / 60; kcat/Km normalized_value=26.266666666666666 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=1600+/-500 min^-1 mM^-1; Km normalized_value=0.075 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=118+/-2 raw_unit=min^-1; kcat/Km raw_value=1600+/-500 raw_unit=min^-1 mM^-1; Km raw_value=0.075+/-0.004 raw_unit=mM


column_label: Table 4. CAR activity against heterocycles and fatty acids.; row msCAR (Mycobacterium smegmatis) / Thiophene-2-carboxylic acid (17); kcat column kcat [min^-1] raw 123+/-4; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 37+/-3; Km column Km [mM] raw 3.3+/-0.3

normalized_value_unit_evidence: kcat normalized_value=2.05 normalized_unit=s^-1 from 123+/-4 / 60; kcat/Km normalized_value=0.6212121212121212 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=37+/-3 min^-1 mM^-1; Km normalized_value=3.3 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=123+/-4 raw_unit=min^-1; kcat/Km raw_value=37+/-3 raw_unit=min^-1 mM^-1; Km raw_value=3.3+/-0.3 raw_unit=mM


substrate_name: Butanoate

column_label: Table 4. CAR activity against heterocycles and fatty acids.; row msCAR (Mycobacterium smegmatis) / Butanoic acid (18); kcat column kcat [min^-1] raw 129+/-7*; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 16+/-2*; Km column Km [mM] raw 7.9+/-0.8*

normalized_value_unit_evidence: kcat normalized_value=2.15 normalized_unit=s^-1 from 129+/-7* / 60; kcat/Km normalized_value=0.27215189873417717 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=16+/-2* min^-1 mM^-1; Km normalized_value=7.9 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=129+/-7* raw_unit=min^-1; kcat/Km raw_value=16+/-2* raw_unit=min^-1 mM^-1; Km raw_value=7.9+/-0.8* raw_unit=mM

substrate: Butanoate


column_label: Table 4. CAR activity against heterocycles and fatty acids.; row msCAR (Mycobacterium smegmatis) / Dodecanoic acid (20); kcat column kcat [min^-1] raw 131+/-5; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 2700+/-400; Km column Km [mM] raw 0.05+/-0.01

normalized_value_unit_evidence: kcat normalized_value=2.18 normalized_unit=s^-1 from 131+/-5 / 60; kcat/Km normalized_value=43.6 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=2700+/-400 min^-1 mM^-1; Km normalized_value=0.05 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=131+/-5 raw_unit=min^-1; kcat/Km raw_value=2700+/-400 raw_unit=min^-1 mM^-1; Km raw_value=0.05+/-0.01 raw_unit=mM


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row msCAR (Mycobacterium smegmatis) / 4-Methylbenzoic acid (2); kcat column kcat [min^-1] raw 154+/-6; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 900+/-100; Km column Km [mM] raw 0.16+/-0.02

normalized_value_unit_evidence: kcat normalized_value=2.57 normalized_unit=s^-1 from 154+/-6 / 60; kcat/Km normalized_value=16.0625 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=900+/-100 min^-1 mM^-1; Km normalized_value=0.16 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=154+/-6 raw_unit=min^-1; kcat/Km raw_value=900+/-100 raw_unit=min^-1 mM^-1; Km raw_value=0.16+/-0.02 raw_unit=mM


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row msCAR (Mycobacterium smegmatis) / 4-Methoxybenzoic acid (3); kcat column kcat [min^-1] raw 179+/-6; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 930+/-80; Km column Km [mM] raw 0.19+/-0.02

normalized_value_unit_evidence: kcat normalized_value=2.98 normalized_unit=s^-1 from 179+/-6 / 60; kcat/Km normalized_value=15.68421052631579 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=930+/-80 min^-1 mM^-1; Km normalized_value=0.19 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=179+/-6 raw_unit=min^-1; kcat/Km raw_value=930+/-80 raw_unit=min^-1 mM^-1; Km raw_value=0.19+/-0.02 raw_unit=mM


organism: Bacillus subtilis

substrate_name: CoA

assay_conditions_summary: cofactor CoA

substrate: CoA


substrate_name: L-alpha-keto-beta-methylvalerate

substrate: L-alpha-keto-beta-methylvalerate


column_label: Table 4. CAR activity against heterocycles and fatty acids.; row noCAR (Nocardia otitidiscaviarum) / Octadecanoic acid (21); kcat column kcat [min^-1] raw 11+/-1; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 500+/-300; Km column Km [mM] raw 0.02+/-0.01

normalized_value_unit_evidence: kcat normalized_value=0.183 normalized_unit=s^-1 from 11+/-1 / 60; kcat/Km normalized_value=9.15 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=500+/-300 min^-1 mM^-1; Km normalized_value=0.02 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=11+/-1 raw_unit=min^-1; kcat/Km raw_value=500+/-300 raw_unit=min^-1 mM^-1; Km raw_value=0.02+/-0.01 raw_unit=mM


assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADPH

column_label: Table 3; enzyme row Nocardia otitidiscaviarum / trans-2-phenylcyclopropane-1-carboxylate; substrate column trans-2-phenylcyclopropane-1-carboxylate; metric rows kcat, Km, kcat/Km

raw_value_unit_evidence: paper Table 3 cells: kcat=48 min^-1; Km=1 mM; kcat/Km=46 min^-1 mM^-1

table_label: Table 3


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row noCAR (Nocardia otitidiscaviarum) / 3-Nitrobenzoic acid (7); kcat column kcat [min^-1] raw 59+/-2; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 24+/-3; Km column Km [mM] raw 2.5+/-0.3

normalized_value_unit_evidence: kcat normalized_value=0.983 normalized_unit=s^-1 from 59+/-2 / 60; kcat/Km normalized_value=0.3932 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=24+/-3 min^-1 mM^-1; Km normalized_value=2.5 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=59+/-2 raw_unit=min^-1; kcat/Km raw_value=24+/-3 raw_unit=min^-1 mM^-1; Km raw_value=2.5+/-0.3 raw_unit=mM


column_label: Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring.; row noCAR (Nocardia otitidiscaviarum) / 3-Oxo-3-phenylpropanoic acid (12); kcat column kcat [min^-1] raw 63+/-2; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 210+/-20; Km column Km [mM] raw 0.29+/-0.03

normalized_value_unit_evidence: kcat normalized_value=1.05 normalized_unit=s^-1 from 63+/-2 / 60; kcat/Km normalized_value=3.620689655172414 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=210+/-20 min^-1 mM^-1; Km normalized_value=0.29 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=63+/-2 raw_unit=min^-1; kcat/Km raw_value=210+/-20 raw_unit=min^-1 mM^-1; Km raw_value=0.29+/-0.03 raw_unit=mM


column_label: Table 4. CAR activity against heterocycles and fatty acids.; row noCAR (Nocardia otitidiscaviarum) / Pyridine-2-carboxylic acid (14); kcat column kcat [min^-1] raw 76+/-4; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 3.9+/-0.4; Km column Km [mM] raw 20+/-2

normalized_value_unit_evidence: kcat normalized_value=1.27 normalized_unit=s^-1 from 76+/-4 / 60; kcat/Km normalized_value=0.0635 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=3.9+/-0.4 min^-1 mM^-1; Km normalized_value=20.0 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=76+/-4 raw_unit=min^-1; kcat/Km raw_value=3.9+/-0.4 raw_unit=min^-1 mM^-1; Km raw_value=20+/-2 raw_unit=mM


column_label: Table 4. CAR activity against heterocycles and fatty acids.; row noCAR (Nocardia otitidiscaviarum) / Dodecanoic acid (20); kcat column kcat [min^-1] raw 99+/-3; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 2500+/-300; Km column Km [mM] raw 0.04+/-0.01

normalized_value_unit_evidence: kcat normalized_value=1.65 normalized_unit=s^-1 from 99+/-3 / 60; kcat/Km normalized_value=41.25 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=2500+/-300 min^-1 mM^-1; Km normalized_value=0.04 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=99+/-3 raw_unit=min^-1; kcat/Km raw_value=2500+/-300 raw_unit=min^-1 mM^-1; Km raw_value=0.04+/-0.01 raw_unit=mM


column_label: Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring.; row noCAR (Nocardia otitidiscaviarum) / (E)-3-Phenylprop-2-enoic acid (10); kcat column kcat [min^-1] raw 105+/-3; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 147+/-15; Km column Km [mM] raw 0.72+/-0.07

normalized_value_unit_evidence: kcat normalized_value=1.75 normalized_unit=s^-1 from 105+/-3 / 60; kcat/Km normalized_value=2.430555555555556 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=147+/-15 min^-1 mM^-1; Km normalized_value=0.72 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=105+/-3 raw_unit=min^-1; kcat/Km raw_value=147+/-15 raw_unit=min^-1 mM^-1; Km raw_value=0.72+/-0.07 raw_unit=mM


column_label: Table 4. CAR activity against heterocycles and fatty acids.; row noCAR (Nocardia otitidiscaviarum) / Thiophene-2-carboxylic acid (17); kcat column kcat [min^-1] raw 135+/-4; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 52+/-4; Km column Km [mM] raw 2.6+/-0.2

normalized_value_unit_evidence: kcat normalized_value=2.25 normalized_unit=s^-1 from 135+/-4 / 60; kcat/Km normalized_value=0.8653846153846153 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=52+/-4 min^-1 mM^-1; Km normalized_value=2.6 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=135+/-4 raw_unit=min^-1; kcat/Km raw_value=52+/-4 raw_unit=min^-1 mM^-1; Km raw_value=2.6+/-0.2 raw_unit=mM


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row noCAR (Nocardia otitidiscaviarum) / 3-Methoxybenzoic acid (4); kcat column kcat [min^-1] raw 136+/-5; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 150+/-10; Km column Km [mM] raw 0.9+/-0.1

normalized_value_unit_evidence: kcat normalized_value=2.27 normalized_unit=s^-1 from 136+/-5 / 60; kcat/Km normalized_value=2.522222222222222 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=150+/-10 min^-1 mM^-1; Km normalized_value=0.9 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=136+/-5 raw_unit=min^-1; kcat/Km raw_value=150+/-10 raw_unit=min^-1 mM^-1; Km raw_value=0.9+/-0.1 raw_unit=mM


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups row noCAR (Nocardia otitidiscaviarum), substrate column 4-Methylbenzoic acid (2), metric row k cat [min^-1], raw cell 135+/-5, normalized 2.25 s^-1; Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups row noCAR (Nocardia otitidiscaviarum), substrate column 4-Methylbenzoic acid (2), metric row k cat/K_m [min^-1 mM^-1], raw cell 110+/-20, normalized 1.83333333333333333333333333333 s^-1*mM^-1; Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups row noCAR (Nocardia otitidiscaviarum), substrate column 4-Methylbenzoic acid (2), metric row K_m [mM], raw cell 1.2+/-0.2, normalized 1.2 mM

normalized_value_unit_evidence: kcat normalized_value=2.25 normalized_unit=s^-1; kcat/Km normalized_value=1.83333333333333333333333333333 normalized_unit=s^-1*mM^-1; Km normalized_value=1.2 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=135 raw_unit=min^-1 raw_cell=135+/-5; kcat/Km raw_value=110 raw_unit=min^-1*mM^-1 raw_cell=110+/-20; Km raw_value=1.2 raw_unit=mM raw_cell=1.2+/-0.2

row_label: noCAR (Nocardia otitidiscaviarum) / 4-Methylbenzoic acid (2)


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row noCAR (Nocardia otitidiscaviarum) / 4-Methoxybenzoic acid (3); kcat column kcat [min^-1] raw 138+/-4; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 130+/-10; Km column Km [mM] raw 1.1+/-0.1

normalized_value_unit_evidence: kcat normalized_value=2.3 normalized_unit=s^-1 from 138+/-4 / 60; kcat/Km normalized_value=2.0909090909090904 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=130+/-10 min^-1 mM^-1; Km normalized_value=1.1 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=138+/-4 raw_unit=min^-1; kcat/Km raw_value=130+/-10 raw_unit=min^-1 mM^-1; Km raw_value=1.1+/-0.1 raw_unit=mM


column_label: Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring.; row noCAR (Nocardia otitidiscaviarum) / 3-Phenylpropionic acid (9); kcat column kcat [min^-1] raw 140+/-4; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 52+/-4; Km column Km [mM] raw 2.7+/-0.2

normalized_value_unit_evidence: kcat normalized_value=2.33 normalized_unit=s^-1 from 140+/-4 / 60; kcat/Km normalized_value=0.8629629629629629 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=52+/-4 min^-1 mM^-1; Km normalized_value=2.7 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=140+/-4 raw_unit=min^-1; kcat/Km raw_value=52+/-4 raw_unit=min^-1 mM^-1; Km raw_value=2.7+/-0.2 raw_unit=mM


organism: Sorbus aucuparia


substrate_name: NAD+

assay_conditions_summary: cofactor NAD+

substrate: NAD+


organism: Felis catus

smiles: C(=CC(=O)C(=O)O)C=CO


substrate_name: K+

substrate: K+


organism: Pyrus pyrifolia

substrate_name: benzaldehyde

substrate: benzaldehyde


assay_conditions_summary: pH 9.5; 313.15 K; cofactor NAD+


organism: Mycobacterium marinum

substrate_name: NADPH

proof_lines: pH and temperature not specified in the publication

substrate: NADPH


substrate_name: benzoic acid

substrate: benzoic acid


organism: Pseudomonas putida

substrate_name: DELTA1-piperidine 6-carboxylate

substrate: DELTA1-piperidine 6-carboxylate


organism: Ectopseudomonas oleovorans


enzyme_display_name: 2,5-dioxovalerate dehydrogenase

organism: Azospirillum brasilense

substrate_name: NADP+

sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q1JUP4 and applied mutation signature R334Q/A337R/A442P/P444T to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 8; 298.15 K; buffer A340 in 100 mM phosphate; cofactor NADP+

column_label: Table 2 (Kinetic characterization of KGSADH enzymes at pH 8), clone row '106-QR'; NADP+ column group: column 'kcat (s^-1)' = 4.0(±1.7); column 'Km (mM)' = 0.54(±0.038); column 'kcat/Km (s^-1 mM^-1)' = 7.2(±3.6).

normalized_value_unit_evidence: kcat: printed Table 2 cell 4.0 s^-1; already in s^-1, no conversion (this paper prints no min^-1 kinetic cell); the exported value is 4.0 s^(-1). Km: printed Table 2 cell 0.54 mM; already in mM, no conversion (this paper prints no uM kinetic cell); the exported value is 0.54 mM. kcat/Km: printed Table 2 cell 7.2 s^-1 mM^-1; the printed unit is already the canonical s^(-1)*mM^(-1), so no conversion is applied and the paper-side value is 7.2 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, clone row '106-QR', NADP+ column group: kcat = 4.0 s^-1; Km = 0.54 mM; printed kcat/Km = 7.2 s^-1 mM^-1.

table_label: Table 2: Kinetic characterization of KGSADH enzymes at pH 8 (Scientific Reports 2017, 7:17155)

enzyme: 2,5-dioxovalerate dehydrogenase

substrate: NADP+


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature R334Q/A337R to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 8; cofactor NADP+

proof_lines: pH 8.0, 25°C, recombinant mutant R334Q/A337R


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q1JUP4 and applied mutation signature K273A/R334Q/A337R/A442P/T443E/P444A to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic characterization of KGSADH enzymes at pH 8), clone row '108-QR'; NADP+ column group: column 'kcat (s^-1)' = 4.5(±1.1); column 'Km (mM)' = 0.66(±0.065); column 'kcat/Km (s^-1 mM^-1)' = 6.9(±2.3).

normalized_value_unit_evidence: kcat: printed Table 2 cell 4.5 s^-1; already in s^-1, no conversion (this paper prints no min^-1 kinetic cell); the exported value is 4.5 s^(-1). Km: printed Table 2 cell 0.66 mM; already in mM, no conversion (this paper prints no uM kinetic cell); the exported value is 0.66 mM. kcat/Km: printed Table 2 cell 6.9 s^-1 mM^-1; the printed unit is already the canonical s^(-1)*mM^(-1), so no conversion is applied and the paper-side value is 6.9 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, clone row '108-QR', NADP+ column group: kcat = 4.5 s^-1; Km = 0.66 mM; printed kcat/Km = 6.9 s^-1 mM^-1.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q1JUP4 and applied mutation signature A110S/K273A/R334Q/A337R/A442P/P444T to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic characterization of KGSADH enzymes at pH 8), clone row '104-QR'; NADP+ column group: column 'kcat (s^-1)' = 4.7(±0.79); column 'Km (mM)' = 0.70(±0.075); column 'kcat/Km (s^-1 mM^-1)' = 6.8(±1.8).

normalized_value_unit_evidence: kcat: printed Table 2 cell 4.7 s^-1; already in s^-1, no conversion (this paper prints no min^-1 kinetic cell); the exported value is 4.7 s^(-1). Km: printed Table 2 cell 0.70 mM; already in mM, no conversion (this paper prints no uM kinetic cell); the exported value is 0.7 mM. kcat/Km: printed Table 2 cell 6.8 s^-1 mM^-1; the printed unit is already the canonical s^(-1)*mM^(-1), so no conversion is applied and the paper-side value is 6.8 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, clone row '104-QR', NADP+ column group: kcat = 4.7 s^-1; Km = 0.70 mM; printed kcat/Km = 6.8 s^-1 mM^-1.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature R334Q/A337R/A442P/P444T to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 8; cofactor NAD+

proof_lines: pH 8.0, 25°C, recombinant mutant R334Q/A337R/A442P/P444T


smiles: C(CO)C=O

assay_conditions_summary: pH 8


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature A110S/K273A/R334Q/A337R/A442P/P444T to reconstruct the assayed variant sequence.

proof_lines: pH 8.0, 25°C, recombinant mutant A110S/K273A/R334Q/A337R/A442P/P444T


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature K273A/R334Q/A337R/A442P/T443E/P444A to reconstruct the assayed variant sequence.

proof_lines: pH 8.0, 25°C, recombinant mutant K273A/R334Q/A337R/A442P/T443E/P444A


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature K273A/A442P/T443E/P444A to reconstruct the assayed variant sequence.

proof_lines: pH 8.0, 25°C, recombinant mutant K273A/A442P/T443E/P444A


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature A442P/P444T to reconstruct the assayed variant sequence.

proof_lines: pH 8.0, 25°C, recombinant mutant A442P/P444T


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature A110S/K273A/A442P/P444T to reconstruct the assayed variant sequence.

proof_lines: pH 8.0, 25°C, recombinant mutant A110S/K273A/A442P/P444T


organism: Antirrhinum majus

substrate_name: phenylacetaldehyde

proof_lines: kcat is 0.31 sec(-1) with benzaldehyde as substrate (PubMed:19292760). kcat is 2.08 sec(-1) with acetaldehyde as substrate (PubMed:19292760). kcat is 0.23 sec(-1) with phenylacetaldehyde as substrate (PubMed:19292760).

source_databases_merged: brenda uniprot

substrate: phenylacetaldehyde


substrate_name: acetaldehyde

smiles: CC=O

substrate: acetaldehyde


enzyme_display_name: Aldehyde dehydrogenase 1A1

organism: Bos taurus

substrate_name: all-trans retinal

proof_lines: Has a much higher catalytic efficiency toward all-trans retinal.

enzyme: Aldehyde dehydrogenase 1A1

substrate: all-trans retinal


substrate_name: propanal

smiles: CCC=O

substrate: propanal


organism: Bacillus subtilis (strain 168)

substrate_name: NAD(+) (at 25 degrees Celsius and pH 7.2)

assay_conditions_summary: pH 7.2; cofactor NAD+

proof_lines: kcat is 2.75 sec(-1) with alpha-ketoglutaric semialdehyde as substrate (in the presence of NAD(+)). kcat is 1.12 sec(-1) with alpha-ketoglutaric semialdehyde as substrate (in the presence of NADP(+)). kcat is 1.29 sec(-1) with propionaldehy


substrate_name: NADP(+) (at 25 degrees Celsius and pH 7.2)


enzyme_display_name: Malonate-semialdehyde dehydrogenase

substrate_name: malonate semialdehyde (at 30 degrees Celsius and pH 8.2)

proof_lines: kcat is 7.4 sec(-1) with malonate semialdehyde as substrate. kcat is 1.1 sec(-1) with methylmalonate semialdehyde as substrate.

enzyme: Malonate-semialdehyde dehydrogenase


substrate_name: methylmalonate semialdehyde (at 30 degrees Celsius and pH 8.2)

smiles: CC(C=O)C(=O)O


substrate_name: CoA (with malonate semialdehyde at 30 degrees Celsius and pH 8.2)

assay_conditions_summary: pH 8.2; cofactor CoA


substrate_name: NAD (with malonate semialdehyde at 30 degrees Celsius and pH 8.2)


enzyme_display_name: Alpha-ketoglutaric semialdehyde dehydrogenase 3

proof_lines: kcat is 21.3 sec(-1) with alpha-ketoglutaric semialdehyde as substrate (in the presence of NAD(+)). kcat is 23.0 sec(-1) with alpha-ketoglutaric semialdehyde as substrate (in the presence of NADP(+)).

substrate: NAD(+) (at 25 degrees Celsius and pH 7.2)


enzyme: Alpha-ketoglutaric semialdehyde dehydrogenase 3

substrate: NADP(+) (at 25 degrees Celsius and pH 7.2)


enzyme_display_name: Alpha-ketoglutaric semialdehyde dehydrogenase 2

proof_lines: kcat is 53.9 sec(-1) with alpha-ketoglutaric semialdehyde as substrate (in the presence of NAD(+)). kcat is 15.4 sec(-1) with alpha-ketoglutaric semialdehyde as substrate (in the presence of NADP(+)).


enzyme: Alpha-ketoglutaric semialdehyde dehydrogenase 2


organism: Saccharolobus solfataricus

substrate_name: glycolaldehyde

smiles: C(C=O)O

proof_lines: pH 6.0, 70°C, native enzyme form xylose-grown cells

substrate: glycolaldehyde


enzyme_display_name: Carboxylic acid reductase

organism: Nocardia iowensis

substrate_name: ATP

proof_lines: Vmax is 3 times higher than that of the natural enzyme when recombinant apo-Car is incubated with the purified PPTase Npt and CoA.

enzyme: Carboxylic acid reductase

substrate: ATP


organism: Rattus norvegicus

substrate_name: 9-cis retinal (at pH 7.5 and 25 degrees Celsius)

proof_lines: Has more than 2-fold higher catalytic efficiency for 9-cis retinal compared to all-trans retinal and 11-cis retinal.


organism: Acinetobacter calcoaceticus

substrate_name: NAD+


organism: Rhodococcus ruber

substrate_name: benzaldehyde


organism: Macaca fascicularis

substrate_name: all-trans retinal (at pH 7.5 and 25 degrees Celsius)

proof_lines: Has a nearly 2-fold higher catalytic efficiency for 9-cis retinal compared to all-trans and 13-cis retinal.


substrate_name: benzaldehyde (with NAD)

proof_lines: kcat is 8240 min(-1) with benzaldehyde and NAD as substrate. kcat is 2550 min(-1) with benzaldehyde and NADP as substrate.

substrate: benzaldehyde (with NAD)


organism: Oryctolagus cuniculus

smiles: CCCCCC(C=CC=O)O


substrate_name: malonaldehyde

smiles: C(C=O)C=O

substrate: malonaldehyde


substrate_name: hexanal

substrate: hexanal


substrate_name: benzoate

sequence_source: multiple

assay_conditions_summary: pH 7.5; 303.15 K; cofactor CoA

source_databases_merged: brenda skid uniprot

substrate: benzoate


enzyme_display_name: L-2-aminoadipate reductase

organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: NADPH

enzyme: L-2-aminoadipate reductase


organism: Burkholderia cepacia

assay_conditions_summary: pH 8.5; cofactor NAD+

proof_lines: pH 8.5, 25°C, with 2-hydroxy-5-methyl-muconic-6-semialdehyde as substrate

proof_lines: pH 8.5, 25°C, with 2-hydroxymuconate-6-semialdehyde as substrate

proof_lines: pH 8.5, 25°C, with 5-chloro-2-hydroxymuconic-6-semialdehyde as substrate

substrate: NAD+


organism: Mus musculus

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

proof_lines: Activity was measured with total soluble protein.


organism: Neurospora crassa

proof_lines: reduction of the intermediate benzoyladenylate to benzaldehyde

substrate: NADPH


organism: Homo sapiens

substrate_name: 2-aminomuconate 6-semialdehyde

smiles: C(=CC=O)C=C(C(=O)O)N

proof_lines: wild type enzyme, pH and temperature not specified in the publication

substrate: 2-aminomuconate 6-semialdehyde


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature R109A to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme R109A, pH and temperature not specified in the publication


substrate_name: dodecanoate

column_label: Table 4. CAR activity against heterocycles and fatty acids.; row niCAR (Nocardia iowensis) / Dodecanoic acid (20); kcat column kcat [min^-1] raw 157+/-9; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 7000+/-2000; Km column Km [mM] raw 0.02+/-0.01

normalized_value_unit_evidence: kcat normalized_value=2.62 normalized_unit=s^-1 from 157+/-9 / 60; kcat/Km normalized_value=131.0 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=7000+/-2000 min^-1 mM^-1; Km normalized_value=0.02 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=157+/-9 raw_unit=min^-1; kcat/Km raw_value=7000+/-2000 raw_unit=min^-1 mM^-1; Km raw_value=0.02+/-0.01 raw_unit=mM

source_databases_merged: brenda skid

substrate: dodecanoate


substrate_name: Octanoate

column_label: Table 4. CAR activity against heterocycles and fatty acids.; row niCAR (Nocardia iowensis) / Octanoic acid (19); kcat column kcat [min^-1] raw 233+/-5; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 1350+/-90; Km column Km [mM] raw 0.2+/-0.01

normalized_value_unit_evidence: kcat normalized_value=3.88 normalized_unit=s^-1 from 233+/-5 / 60; kcat/Km normalized_value=19.4 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=1350+/-90 min^-1 mM^-1; Km normalized_value=0.2 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=233+/-5 raw_unit=min^-1; kcat/Km raw_value=1350+/-90 raw_unit=min^-1 mM^-1; Km raw_value=0.2+/-0.01 raw_unit=mM

substrate: Octanoate


substrate_name: Butanoate

column_label: Table 4. CAR activity against heterocycles and fatty acids.; row niCAR (Nocardia iowensis) / Butanoic acid (18); kcat column kcat [min^-1] raw 260+/-30*; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 8+/-1*; Km column Km [mM] raw 32+/-4*

normalized_value_unit_evidence: kcat normalized_value=4.33 normalized_unit=s^-1 from 260+/-30* / 60; kcat/Km normalized_value=0.1353125 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=8+/-1* min^-1 mM^-1; Km normalized_value=32.0 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=260+/-30* raw_unit=min^-1; kcat/Km raw_value=8+/-1* raw_unit=min^-1 mM^-1; Km raw_value=32+/-4* raw_unit=mM

substrate: Butanoate


enzyme_display_name: carboxylate reductase (NADP(+))

column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row niCAR (Nocardia iowensis) / 3-Methoxybenzoic acid (4); kcat column kcat [min^-1] raw 93+/-1; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 137+/-6; Km column Km [mM] raw 0.68+/-0.03

normalized_value_unit_evidence: kcat normalized_value=1.55 normalized_unit=s^-1 from 93+/-1 / 60; kcat/Km normalized_value=2.2794117647058822 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=137+/-6 min^-1 mM^-1; Km normalized_value=0.68 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=93+/-1 raw_unit=min^-1; kcat/Km raw_value=137+/-6 raw_unit=min^-1 mM^-1; Km raw_value=0.68+/-0.03 raw_unit=mM

enzyme: carboxylate reductase (NADP(+))


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row niCAR (Nocardia iowensis) / 3-Nitrobenzoic acid (7); kcat column kcat [min^-1] raw 18+/-1; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 3.2+/-0.4; Km column Km [mM] raw 5.6+/-0.7

normalized_value_unit_evidence: kcat normalized_value=0.3 normalized_unit=s^-1 from 18+/-1 / 60; kcat/Km normalized_value=0.053571428571428575 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=3.2+/-0.4 min^-1 mM^-1; Km normalized_value=5.6 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=18+/-1 raw_unit=min^-1; kcat/Km raw_value=3.2+/-0.4 raw_unit=min^-1 mM^-1; Km raw_value=5.6+/-0.7 raw_unit=mM


column_label: Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring.; row niCAR (Nocardia iowensis) / 3-Oxo-3-phenylpropanoic acid (12); kcat column kcat [min^-1] raw 37.1+/-0.5; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 94+/-4; Km column Km [mM] raw 0.39+/-0.02

normalized_value_unit_evidence: kcat normalized_value=0.618 normalized_unit=s^-1 from 37.1+/-0.5 / 60; kcat/Km normalized_value=1.5846153846153845 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=94+/-4 min^-1 mM^-1; Km normalized_value=0.39 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=37.1+/-0.5 raw_unit=min^-1; kcat/Km raw_value=94+/-4 raw_unit=min^-1 mM^-1; Km raw_value=0.39+/-0.02 raw_unit=mM


column_label: Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring.; row niCAR (Nocardia iowensis) / Phenylpropynoic acid (11); kcat column kcat [min^-1] raw 7+/-0.4; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 5+/-1; Km column Km [mM] raw 1.3+/-0.2

normalized_value_unit_evidence: kcat normalized_value=0.117 normalized_unit=s^-1 from 7+/-0.4 / 60; kcat/Km normalized_value=0.09 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=5+/-1 min^-1 mM^-1; Km normalized_value=1.3 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=7+/-0.4 raw_unit=min^-1; kcat/Km raw_value=5+/-1 raw_unit=min^-1 mM^-1; Km raw_value=1.3+/-0.2 raw_unit=mM


column_label: Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring.; row niCAR (Nocardia iowensis) / 3-Phenylpropionic acid (9); kcat column kcat [min^-1] raw 85.8+/-0.9; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 88+/-3; Km column Km [mM] raw 0.97+/-0.03

normalized_value_unit_evidence: kcat normalized_value=1.43 normalized_unit=s^-1 from 85.8+/-0.9 / 60; kcat/Km normalized_value=1.4742268041237112 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=88+/-3 min^-1 mM^-1; Km normalized_value=0.97 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=85.8+/-0.9 raw_unit=min^-1; kcat/Km raw_value=88+/-3 raw_unit=min^-1 mM^-1; Km raw_value=0.97+/-0.03 raw_unit=mM


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row niCAR (Nocardia iowensis) / 4-Methoxybenzoic acid (3); kcat column kcat [min^-1] raw 49+/-1; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 200+/-10; Km column Km [mM] raw 0.25+/-0.01

normalized_value_unit_evidence: kcat normalized_value=0.82 normalized_unit=s^-1 from 49+/-1 / 60; kcat/Km normalized_value=3.28 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=200+/-10 min^-1 mM^-1; Km normalized_value=0.25 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=49+/-1 raw_unit=min^-1; kcat/Km raw_value=200+/-10 raw_unit=min^-1 mM^-1; Km raw_value=0.25+/-0.01 raw_unit=mM


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row niCAR (Nocardia iowensis) / 4-Methylbenzoic acid (2); kcat column kcat [min^-1] raw 94+/-2; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 97+/-6; Km column Km [mM] raw 1.0+/-0.1

normalized_value_unit_evidence: kcat normalized_value=1.57 normalized_unit=s^-1 from 94+/-2 / 60; kcat/Km normalized_value=1.57 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=97+/-6 min^-1 mM^-1; Km normalized_value=1.0 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=94+/-2 raw_unit=min^-1; kcat/Km raw_value=97+/-6 raw_unit=min^-1 mM^-1; Km raw_value=1.0+/-0.1 raw_unit=mM


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row niCAR (Nocardia iowensis) / Benzoic acid (1); kcat column kcat [min^-1] raw 98+/-7; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 103+/-9; Km column Km [mM] raw 0.9+/-0.1

normalized_value_unit_evidence: kcat normalized_value=1.63 normalized_unit=s^-1 from 98+/-7 / 60; kcat/Km normalized_value=1.811111111111111 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=103+/-9 min^-1 mM^-1; Km normalized_value=0.9 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=98+/-7 raw_unit=min^-1; kcat/Km raw_value=103+/-9 raw_unit=min^-1 mM^-1; Km raw_value=0.9+/-0.1 raw_unit=mM


smiles: CCCC(=O)O

row_label: niCAR (Nocardia iowensis) / Butanoic acid (18)

table_label: Table 4. CAR activity against heterocycles and fatty acids.


smiles: CCCCCCCCCCCC(=O)O

row_label: niCAR (Nocardia iowensis) / Dodecanoic acid (20)


column_label: Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring.; row niCAR (Nocardia iowensis) / (E)-3-Phenylprop-2-enoic acid (10); kcat column kcat [min^-1] raw 7.7+/-0.7; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 170+/-70; Km column Km [mM] raw 0.05+/-0.02

normalized_value_unit_evidence: kcat normalized_value=0.128 normalized_unit=s^-1 from 7.7+/-0.7 / 60; kcat/Km normalized_value=2.56 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=170+/-70 min^-1 mM^-1; Km normalized_value=0.05 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=7.7+/-0.7 raw_unit=min^-1; kcat/Km raw_value=170+/-70 raw_unit=min^-1 mM^-1; Km raw_value=0.05+/-0.02 raw_unit=mM


substrate_name: octadecanoate

smiles: CCCCCCCCCCCCCCCCCC(=O)O

column_label: Table 4. CAR activity against heterocycles and fatty acids.; row niCAR (Nocardia iowensis) / Octadecanoic acid (21); kcat column kcat [min^-1] raw 68+/-7; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 100+/-20; Km column Km [mM] raw 0.7+/-0.1

normalized_value_unit_evidence: kcat normalized_value=1.13 normalized_unit=s^-1 from 68+/-7 / 60; kcat/Km normalized_value=1.6142857142857143 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=100+/-20 min^-1 mM^-1; Km normalized_value=0.7 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=68+/-7 raw_unit=min^-1; kcat/Km raw_value=100+/-20 raw_unit=min^-1 mM^-1; Km raw_value=0.7+/-0.1 raw_unit=mM

substrate: octadecanoate


row_label: niCAR (Nocardia iowensis) / Octanoic acid (19)


column_label: Table 4. CAR activity against heterocycles and fatty acids.; row niCAR (Nocardia iowensis) / Thiophene-2-carboxylic acid (17); kcat column kcat [min^-1] raw 60.8+/-0.9; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 58+/-3; Km column Km [mM] raw 1.00+/-0.05

normalized_value_unit_evidence: kcat normalized_value=1.01 normalized_unit=s^-1 from 60.8+/-0.9 / 60; kcat/Km normalized_value=1.01 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=58+/-3 min^-1 mM^-1; Km normalized_value=1.0 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=60.8+/-0.9 raw_unit=min^-1; kcat/Km raw_value=58+/-3 raw_unit=min^-1 mM^-1; Km raw_value=1.00+/-0.05 raw_unit=mM


column_label: Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring.; row niCAR (Nocardia iowensis) / trans-2-Phenylcyclopropane-1-carboxylic acid (13); kcat column kcat [min^-1] raw 10.8+/-0.3; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 51+/-5; Km column Km [mM] raw 0.21+/-0.02

normalized_value_unit_evidence: kcat normalized_value=0.18 normalized_unit=s^-1 from 10.8+/-0.3 / 60; kcat/Km normalized_value=0.8571428571428571 normalized_unit=s^-1*mM^-1 from same-row kcat and Km; printed efficiency=51+/-5 min^-1 mM^-1; Km normalized_value=0.21 normalized_unit=mM

raw_value_unit_evidence: kcat raw_value=10.8+/-0.3 raw_unit=min^-1; kcat/Km raw_value=51+/-5 raw_unit=min^-1 mM^-1; Km raw_value=0.21+/-0.02 raw_unit=mM


enzyme_display_name: benzaldehyde dehydrogenase (NAD(+))

organism: Pseudomonas putida

assay_conditions_summary: pH 8.5; 303.15 K; cofactor NAD+

column_label: Article PDF page 7, Table III, row p-chlorobenzaldehyde, columns Km (uM), kcat (s^-1), and kcat/Km (M^-1 s^-1); cells 1.2 +/- 0.2, 135 +/- 2, and 11 x 10^7.

raw_value_unit_evidence: Table III row p-chlorobenzaldehyde: Km 1.2 +/- 0.2 uM; kcat 135 +/- 2 s^-1; kcat/Km 11 x 10^7 M^-1 s^-1.

row_label: p-chlorobenzaldehyde

table_label: Table III. Reaction of PpBADH with substituted benzaldehydes

enzyme: benzaldehyde dehydrogenase (NAD(+))


column_label: Article PDF page 7, Table III, row p-nitrobenzaldehyde, columns Km (uM), kcat (s^-1), and kcat/Km (M^-1 s^-1); cells 9.3 +/- 0.8, 95 +/- 3, and 1.0 x 10^7.

raw_value_unit_evidence: Table III row p-nitrobenzaldehyde: Km 9.3 +/- 0.8 uM; kcat 95 +/- 3 s^-1; kcat/Km 1.0 x 10^7 M^-1 s^-1.

row_label: p-nitrobenzaldehyde


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature E215D to reconstruct the assayed variant sequence.

column_label: Article PDF page 7, Table II, PpBADH row E215D, columns Km (uM), kcat (s^-1), and kcat/Km (M^-1 s^-1); cells 28 +/- 3.9, 1.5 +/- 0.2, and 5.4 x 10^4.

normalized_value_unit_evidence: Km 0.028 mM; kcat 1.5 s^-1; printed kcat/Km 54 s^-1 mM^-1.

row_label: PpBADH E215D

table_label: Table II. Comparison of kinetic parameters of PpBADH variants and selected other ALDHs

substrate: benzaldehyde


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature E337D to reconstruct the assayed variant sequence.

column_label: Article PDF page 7, Table II, PpBADH row E337D, columns Km (uM), kcat (s^-1), and kcat/Km (M^-1 s^-1); cells 6.6 +/- 0.7, 45 +/- 1, and 6.8 x 10^6.

normalized_value_unit_evidence: Km 0.0066 mM; kcat 45 s^-1; printed kcat/Km 6800 s^-1 mM^-1.

row_label: PpBADH E337D


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature E337L to reconstruct the assayed variant sequence.

column_label: Article PDF page 7, Table II, PpBADH row E337L, columns Km (uM), kcat (s^-1), and kcat/Km (M^-1 s^-1); cells 20 +/- 1.5, 1.3 +/- 0.1, and 6.5 x 10^4.

normalized_value_unit_evidence: Km 0.02 mM; kcat 1.3 s^-1; printed kcat/Km 65 s^-1 mM^-1.

row_label: PpBADH E337L


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature E337Q to reconstruct the assayed variant sequence.

column_label: Article PDF page 7, Table II, PpBADH row E337Q, columns Km (uM), kcat (s^-1), and kcat/Km (M^-1 s^-1); cells 20 +/- 1.6, 0.8 +/- 0.1, and 4.0 x 10^4.

normalized_value_unit_evidence: Km 0.02 mM; kcat 0.8 s^-1; printed kcat/Km 40 s^-1 mM^-1.

row_label: PpBADH E337Q


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

column_label: Article PDF page 7, Table III, row Benzaldehyde, columns Km (uM), kcat (s^-1), and kcat/Km (M^-1 s^-1); cells 4.4 +/- 2.2, 156 +/- 29, and 3.5 x 10^7.

raw_value_unit_evidence: Table III row Benzaldehyde: Km 4.4 +/- 2.2 uM; kcat 156 +/- 29 s^-1; kcat/Km 3.5 x 10^7 M^-1 s^-1.

row_label: Benzaldehyde


column_label: Article PDF page 7, Table III, row p-methoxybenzaldehyde, columns Km (uM), kcat (s^-1), and kcat/Km (M^-1 s^-1); cells 2.0 +/- 0.3, 116 +/- 4, and 5.8 x 10^7.

raw_value_unit_evidence: Table III row p-methoxybenzaldehyde: Km 2.0 +/- 0.3 uM; kcat 116 +/- 4 s^-1; kcat/Km 5.8 x 10^7 M^-1 s^-1.

row_label: p-methoxybenzaldehyde


column_label: Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring.; row niCAR (Nocardia iowensis) / Phenylpropynoic acid (11); kcat column kcat [min^-1] raw 7+/-0.4; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 5+/-1; Km column Km [mM] raw 1.3+/-0.2


row_label: niCAR (Nocardia iowensis) / (E)-3-Phenylprop-2-enoic acid (10)

table_label: Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring.


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row niCAR (Nocardia iowensis) / 3-Nitrobenzoic acid (7); kcat column kcat [min^-1] raw 18+/-1; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 3.2+/-0.4; Km column Km [mM] raw 5.6+/-0.7


column_label: Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring.; row niCAR (Nocardia iowensis) / 3-Oxo-3-phenylpropanoic acid (12); kcat column kcat [min^-1] raw 37.1+/-0.5; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 94+/-4; Km column Km [mM] raw 0.39+/-0.02


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row niCAR (Nocardia iowensis) / 4-Methoxybenzoic acid (3); kcat column kcat [min^-1] raw 49+/-1; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 200+/-10; Km column Km [mM] raw 0.25+/-0.01


row_label: niCAR (Nocardia iowensis) / Thiophene-2-carboxylic acid (17)


smiles: CCCCCCCCCCCCCCCCCC(=O)[O-]

row_label: niCAR (Nocardia iowensis) / Octadecanoic acid (21)


column_label: Table 3. CAR activity against benzoic acid derivatives with the carboxylic acid group extended from the ring.; row niCAR (Nocardia iowensis) / 3-Phenylpropionic acid (9); kcat column kcat [min^-1] raw 85.8+/-0.9; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 88+/-3; Km column Km [mM] raw 0.97+/-0.03


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row niCAR (Nocardia iowensis) / 3-Methoxybenzoic acid (4); kcat column kcat [min^-1] raw 93+/-1; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 137+/-6; Km column Km [mM] raw 0.68+/-0.03


column_label: Table 2. CAR activity against benzoic acid and its derivatives with electron-donating and -withdrawing groups.; row niCAR (Nocardia iowensis) / 4-Methylbenzoic acid (2); kcat column kcat [min^-1] raw 94+/-2; kcat/Km column kcat/Km [min^-1 mM^-1] plus same-row kcat/Km calculation raw 97+/-6; Km column Km [mM] raw 1.0+/-0.1


substrate_name: nonanal

smiles: CCCCCCCCC=O

column_label: Table 1; row Nonanal; Km column

normalized_value_unit_evidence: Km=0.0285 mM converted from 28.5 uM

raw_value_unit_evidence: Table 1 Nonanal Km 28.5 +/- 4.8 uM

row_label: Nonanal

table_label: Table 1

substrate: nonanal


smiles: CCCCCCC=CC=O

column_label: Table 1; row trans-2-Nonenal; Km column

normalized_value_unit_evidence: Km=0.0053 mM converted from 5.3 uM

raw_value_unit_evidence: Table 1 trans-2-Nonenal Km 5.3 +/- 0.7 uM


smiles: CCCCCC=O

column_label: Table 1; row Hexanal; Km column

normalized_value_unit_evidence: Km=0.0391 mM converted from 39.1 uM

raw_value_unit_evidence: Table 1 Hexanal Km 39.1 +/- 3.9 uM

row_label: Hexanal


substrate_name: octanal

smiles: CCCCCCCC=O

column_label: Table 1; row Octanal; Km column

normalized_value_unit_evidence: Km=0.0175 mM converted from 17.5 uM

raw_value_unit_evidence: Table 1 Octanal Km 17.5 +/- 2.1 uM

row_label: Octanal

substrate: octanal


substrate_name: betaine aldehyde

column_label: Table 1; row Betaine aldehyde; Km column

normalized_value_unit_evidence: Km=0.0411 mM converted from 41.1 uM

raw_value_unit_evidence: Table 1 Betaine aldehyde Km 41.1 +/- 9.3 uM

row_label: Betaine aldehyde

substrate: betaine aldehyde


substrate_name: L-2-aminoadipate 6-semialdehyde

smiles: C(CC=O)CC(C(=O)O)N

column_label: Table 1; row AASA; Km column

normalized_value_unit_evidence: Km=0.169 mM converted from 169 uM

raw_value_unit_evidence: Table 1 AASA Km 169 +/- 9.5 uM

row_label: AASA

substrate: L-2-aminoadipate 6-semialdehyde


column_label: Table 1; row Benzaldehyde; Km column

normalized_value_unit_evidence: Km=0.5302 mM converted from 530.2 uM

raw_value_unit_evidence: Table 1 Benzaldehyde Km 530.2 +/- 86.3 uM


substrate_name: propanal

column_label: Table 1; row Propanal; Km column

normalized_value_unit_evidence: Km=0.6474 mM converted from 647.4 uM

raw_value_unit_evidence: Table 1 Propanal Km 647.4 +/- 38.5 uM

row_label: Propanal

substrate: propanal


substrate_name: glyceraldehyde

smiles: C(C(C=O)O)O

column_label: Table 1; row Glyceraldehyde; Km column

normalized_value_unit_evidence: Km=7.3743 mM converted from 7374.3 uM

raw_value_unit_evidence: Table 1 Glyceraldehyde Km 7374.3 +/- 117.2 uM

row_label: Glyceraldehyde

substrate: glyceraldehyde


organism: Azospirillum brasilense

smiles: O=CCCC(=O)C(=O)O

assay_conditions_summary: pH 7.2; 298.15 K; cofactor NADP+

column_label: Table 1. Kinetic parameters of KGSADH-I, KGSADH-II, KGSADH-III, and ycbD protein for alphaKGSA; row KGSADH-III / NADP+; columns Km (mM); kcat (s^-1); kcat/Km (s^-1*mM^-1); raw table cells: Km 0.0075 ± 0.0009 mM | kcat 23.0 ± 1.0 s^-1 | kcat/Km 3090 ± 250 s^-1*mM^-1

normalized_value_unit_evidence: kcat normalized_value=23 normalized_unit=s^-1; Km normalized_value=0.008 normalized_unit=mM; kcat/Km normalized_value=2875 normalized_unit=s^-1*mM^-1

raw_value_unit_evidence: kcat raw_value=23.0 ± 1.0 raw_unit=s^-1; Km raw_value=0.0075 ± 0.0009 raw_unit=mM; kcat/Km raw_value=3090 ± 250 raw_unit=s^-1*mM^-1

row_label: KGSADH-III / NADP+


assay_conditions_summary: pH 7.2; 298.15 K; cofactor NAD+

column_label: Table 1. Kinetic parameters of KGSADH-I, KGSADH-II, KGSADH-III, and ycbD protein for alphaKGSA; row KGSADH-II / NAD+; columns Km (mM); kcat (s^-1); kcat/Km (s^-1*mM^-1); raw table cells: Km 0.0246 ± 0.004 mM | kcat 53.9 ± 6.1 s^-1 | kcat/Km 2230 ± 176 s^-1*mM^-1

normalized_value_unit_evidence: kcat normalized_value=53.9 normalized_unit=s^-1; Km normalized_value=0.025 normalized_unit=mM; kcat/Km normalized_value=2156 normalized_unit=s^-1*mM^-1

raw_value_unit_evidence: kcat raw_value=53.9 ± 6.1 raw_unit=s^-1; Km raw_value=0.0246 ± 0.004 raw_unit=mM; kcat/Km raw_value=2230 ± 176 raw_unit=s^-1*mM^-1

row_label: KGSADH-II / NAD+


substrate_name: NAD+

column_label: Table 2. Kinetic parameters of KGSADH-I, KGSADH-II, KGSADH-III, and ycbD protein for NAD+ and NADP+; row KGSADH-III / NAD+; columns Km (mM); kcat (s^-1); kcat/Km (s^-1*mM^-1); raw table cells: Km 1.136 ± 0.334 mM | kcat 52.3 ± 12 s^-1 | kcat/Km 47.2 ± 4.3 s^-1*mM^-1

normalized_value_unit_evidence: kcat normalized_value=52.3 normalized_unit=s^-1; Km normalized_value=1.136 normalized_unit=mM; kcat/Km normalized_value=46.0387323943662 normalized_unit=s^-1*mM^-1

raw_value_unit_evidence: kcat raw_value=52.3 ± 12 raw_unit=s^-1; Km raw_value=1.136 ± 0.334 raw_unit=mM; kcat/Km raw_value=47.2 ± 4.3 raw_unit=s^-1*mM^-1

row_label: KGSADH-III / NAD+


column_label: Table 2. Kinetic parameters of KGSADH-I, KGSADH-II, KGSADH-III, and ycbD protein for NAD+ and NADP+; row KGSADH-II / NAD+; columns Km (mM); kcat (s^-1); kcat/Km (s^-1*mM^-1); raw table cells: Km 0.1169 ± 0.017 mM | kcat 35.5 ± 3.2 s^-1 | kcat/Km 307 ± 21 s^-1*mM^-1

normalized_value_unit_evidence: kcat normalized_value=35.5 normalized_unit=s^-1; Km normalized_value=0.117 normalized_unit=mM; kcat/Km normalized_value=303.4188034188034 normalized_unit=s^-1*mM^-1

raw_value_unit_evidence: kcat raw_value=35.5 ± 3.2 raw_unit=s^-1; Km raw_value=0.1169 ± 0.017 raw_unit=mM; kcat/Km raw_value=307 ± 21 raw_unit=s^-1*mM^-1


substrate_name: NADP+

column_label: Table 2. Kinetic parameters of KGSADH-I, KGSADH-II, KGSADH-III, and ycbD protein for NAD+ and NADP+; row KGSADH-III / NADP+; columns Km (mM); kcat (s^-1); kcat/Km (s^-1*mM^-1); raw table cells: Km 0.0149 ± 0.0008 mM | kcat 15.3 ± 0.2 s^-1 | kcat/Km 1030 ± 46 s^-1*mM^-1

normalized_value_unit_evidence: kcat normalized_value=15.3 normalized_unit=s^-1; Km normalized_value=0.015 normalized_unit=mM; kcat/Km normalized_value=1020.0000000000001 normalized_unit=s^-1*mM^-1

raw_value_unit_evidence: kcat raw_value=15.3 ± 0.2 raw_unit=s^-1; Km raw_value=0.0149 ± 0.0008 raw_unit=mM; kcat/Km raw_value=1030 ± 46 raw_unit=s^-1*mM^-1

substrate: NADP+


column_label: Table 2. Kinetic parameters of KGSADH-I, KGSADH-II, KGSADH-III, and ycbD protein for NAD+ and NADP+; row KGSADH-II / NADP+; columns Km (mM); kcat (s^-1); kcat/Km (s^-1*mM^-1); raw table cells: Km 0.0622 ± 0.002 mM | kcat 10.2 ± 0.1 s^-1 | kcat/Km 164 ± 6.6 s^-1*mM^-1

normalized_value_unit_evidence: kcat normalized_value=10.2 normalized_unit=s^-1; Km normalized_value=0.062 normalized_unit=mM; kcat/Km normalized_value=164.51612903225805 normalized_unit=s^-1*mM^-1

raw_value_unit_evidence: kcat raw_value=10.2 ± 0.1 raw_unit=s^-1; Km raw_value=0.0622 ± 0.002 raw_unit=mM; kcat/Km raw_value=164 ± 6.6 raw_unit=s^-1*mM^-1

row_label: KGSADH-II / NADP+