enzyme_display_name: malate synthase

organism: Candida tropicalis

enzyme: malate synthase


substrate_name: glyoxylate

source_db: brenda

verification_status: unverified

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

substrate: glyoxylate

status: unverified

source: brenda


organism: Ricinus communis

substrate_name: acetyl-CoA

assay_conditions_summary: cofactor CoA

substrate: acetyl-CoA


substrate_name: Unknown

km_display: Not reported

ki_unit: mM

substrate: Unknown


organism: Streptomyces coelicolor


organism: Arabidopsis sp.

substrate_name: indole propionic acid

substrate: indole propionic acid


substrate_name: indole butyric acid

substrate: indole butyric acid


substrate_name: Cinnamic acid

substrate: Cinnamic acid


substrate_name: UDPglucose

substrate: UDPglucose


organism: Arabidopsis thaliana


substrate_name: phenylacetic acid

substrate: phenylacetic acid


organism: Gallus gallus

substrate_name: UDP-galactose

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

substrate: UDP-galactose


substrate_name: asialo ovine submaxillary mucin

proof_lines: in terms of N-acetylgalactosamine equivalents

substrate: asialo ovine submaxillary mucin


enzyme_display_name: isoflavone 7-O-glucosyltransferase

organism: Astragalus membranaceus

substrate_name: Calycosin

enzyme: isoflavone 7-O-glucosyltransferase

substrate: Calycosin


organism: Campylobacter concisus

substrate_name: UDP-N-acetyl-D-galactosaminuronic acid

substrate: UDP-N-acetyl-D-galactosaminuronic acid


organism: Acholeplasma laidlawii

substrate_name: UDP-alpha-D-glucose

proof_lines:
pH 8.0, 28°C, presence of 31 mol% CHAPS
pH 8.0, 28°C, presence of 20 mol% CHAPS
pH 8.0, 28°C, presence of 25 mol% CHAPS

substrate: UDP-alpha-D-glucose


enzyme_display_name: phlorizin synthase

organism: Malus domestica

substrate_name: phloretin

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ADP

proof_lines: with ADP-alpha-D-glucose as cosubstrate, at pH 7.5 and 30°C

proof_lines: with UDP-alpha-D-glucose as cosubstrate, at pH 7.5 and 30°C

enzyme: phlorizin synthase

substrate: phloretin


proof_lines: with phloretin as cosubstrate, at pH 7.5 and 30°C


substrate_name: luteolin

substrate: luteolin


substrate_name: isoliquiritigenin

substrate: isoliquiritigenin


substrate_name: kaempferol

substrate: kaempferol


substrate_name: quercetin

proof_lines: with UDP-alpha-D-galactose as cosubstrate, at pH 7.5 and 30°C

substrate: quercetin


substrate_name: ADP-alpha-D-glucose

proof_lines: with quercetin as cosubstrate, at pH 7.5 and 30°C

substrate: ADP-alpha-D-glucose


organism: Homo sapiens

substrate_name: GDP-L-fucose

mutation_type: substitution

substrate: GDP-L-fucose


proof_lines:
wild-type enzyme
wildtype


proof_lines:
reaction with Galbeta(1,4)GlcNAc
reaction with Galbeta(1,3)GlcNAc
reaction with lactose


substrate_name: GDP

substrate: GDP


substrate_name: GMP

substrate: GMP


proof_lines:
enzyme expressed in Sf9 cells
at pH 7.0 and absence of Mn2+
at pH 4.7 and presence of Mn2+


substrate_name: GDP-fucose

proof_lines: mutant W111Y, with Fuc-alpha-1,2Gal-beta-1,3GlcNAc-sp-biotin

substrate: GDP-fucose


proof_lines: mutant W111F, with Fuc-alpha-1,2Gal-beta-1,3GlcNAc-sp-biotin


proof_lines: mutant W111A, with Fuc-alpha-1,2Gal-beta-1,3GlcNAc-sp-biotin


organism: Cricetulus griseus


substrate_name: factor VII EGF-1 domain

substrate: factor VII EGF-1 domain


enzyme_display_name: hexaprenyl-diphosphate synthase [(2E,6E)-farnesyl-diphosphate specific]

organism: Micrococcus luteus

substrate_name: (2E,6E)-farnesyl diphosphate

proof_lines: pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79F)

proof_lines: pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(A79L)

substrate: (2E,6E)-farnesyl diphosphate


substrate_name: geranylgeranyl diphosphate

proof_lines: pH 8.5, 37°C, mutant enzyme subunit-A(wild-type)/subunit-B(V76G)

enzyme: hexaprenyl-diphosphate synthase [(2E,6E)-farnesyl-diphosphate specific]

substrate: geranylgeranyl diphosphate


substrate_name: isopentenyl diphosphate

smiles: CC(=C)CCOP(=O)(O)OP(=O)(O)O

substrate: isopentenyl diphosphate


organism: Bacteria

substrate_name: L-glutamine

smiles: C(CC(=O)N)C(C(=O)O)N

substrate: L-glutamine


organism: Paenarthrobacter aurescens


substrate_name: Unknown

substrate: Unknown


organism: Volvariella volvacea

substrate_name: D-fructose 6-phosphate

proof_lines: recombinant enzyme, in 10 mM KCl, 1 mg/ml bovine serum albumin, 20 mM imidazole buffer (pH 6.8), 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol

substrate: D-fructose 6-phosphate


enzyme_display_name: succinyldiaminopimelate transaminase

organism: Streptomyces avermitilis

substrate_name: prephenate

enzyme: succinyldiaminopimelate transaminase

substrate: prephenate


organism: Candida guilliermondii var. membranaefaciens


smiles: C(CC(=O)O)CN


substrate_name: beta-alanine

smiles: C(CN)C(=O)O

substrate: beta-alanine


organism: Lachancea kluyveri

proof_lines: in 100 mM potassium phosphate (pH 8.0), 0.1 mM pyridoxal 5'-phosphate


assay_conditions_summary: pH 8


substrate_name: gamma-aminobutanoate

substrate: gamma-aminobutanoate


substrate_name: beta-Alanine

substrate: beta-Alanine


substrate_name: glyoxylate

proof_lines: pH 9, 25°C, fixed substrate: 4-aminobutanoate

substrate: glyoxylate


substrate_name: pyruvate

substrate: pyruvate


proof_lines:
pH 9, 25°C, fixed substrate: pyruvate
pH 9, 25°C, fixed substrate: glyoxylate


organism: Mus musculus


enzyme_display_name: hexokinase

organism: Eimeria tenella

substrate_name: D-mannose

enzyme: hexokinase

substrate: D-mannose


substrate_name: D-fructose

substrate: D-fructose


smiles: C(C=O)C(C(C(CO)O)O)O


organism: Kluyveromyces lactis

substrate_name: D-glucose

proof_lines: pH 7.4, 25°C, recombinant enzyme at 0.001 mg/ml

proof_lines: pH 7.4, 25°C, recombinant enzyme at 1.0 mg/ml

substrate: D-glucose


substrate_name: ATP

assay_conditions_summary: pH 7.4; 298.15 K; cofactor ATP

substrate: ATP


organism: Macrobrachium nipponense


assay_conditions_summary: pH 8; 303.15 K; cofactor ATP


organism: Schizosaccharomyces pombe


organism: Aeropyrum pernix

substrate_name: D-glucose 6-phosphate

smiles: C(C(C(C(C(C=O)O)O)O)O)OP(=O)(O)O

substrate: D-glucose 6-phosphate


proof_lines:
pH 6.7, 85°C
pH 6.5, 95°C, discontinuous assay


substrate_name: adenosine

substrate: adenosine


substrate_name: fructose

substrate: fructose


substrate_name: D-ribose 5-phosphate

substrate: D-ribose 5-phosphate


substrate_name: D-ribose

substrate: D-ribose


substrate_name: Unknown

substrate: Unknown


assay_conditions_summary: pH 6.5; 368.15 K; cofactor ATP

proof_lines:
pH 6.5, 95°C, discontinuous assay
pH 6.7, 85°C


organism: Amycolatopsis methanolica

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP


organism: Clostridium thermosuccinogenes

substrate_name: D-fructose 6-phosphate

assay_conditions_summary: pH 7; 328.15 K; cofactor ATP

substrate: D-fructose 6-phosphate


organism: Crassostrea virginica

assay_conditions_summary: pH 7.9; 297.15 K; cofactor ATP


organism: Dictyostelium discoideum

assay_conditions_summary: pH 7; 298.15 K; cofactor ATP

proof_lines: wild type enzyme, at pH 7.0 in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2 and 25°C


proof_lines: pH 7.15, 25°C, in the presence of 0.1 mM fructose 2,6-bisphosphate

proof_lines: pH 7.15, 25°C, in the presence of 1 mM AMP


enzyme_display_name: glucokinase

organism: Caldanaerobacter subterraneus subsp. tengcongensis

enzyme: glucokinase


organism: Lysinibacillus sphaericus C3-41


assay_conditions_summary: pH 9; 303.15 K; cofactor ATP


organism: Saccharomyces cerevisiae


assay_conditions_summary: cofactor ATP


enzyme_display_name: fructokinase

organism: Beta vulgaris

enzyme: fructokinase


enzyme_display_name: galactokinase

organism: Cricetulus griseus

substrate_name: D-galactose

enzyme: galactokinase

substrate: D-galactose


enzyme_display_name: AMP-polyphosphate phosphotransferase

organism: Acinetobacter sp.

substrate_name: (Phosphate)n

smiles: N#N

assay_conditions_summary: cofactor ADP

proof_lines: pH and temperature not specified in the publication, polyphosphate with an average chain length of 35 phosphate groups

enzyme: AMP-polyphosphate phosphotransferase

substrate: (Phosphate)n


substrate_name: AMP

proof_lines: pH and temperature not specified in the publication

substrate: AMP


enzyme_display_name: ribose-phosphate diphosphokinase

organism: Methanocaldococcus jannaschii

enzyme: ribose-phosphate diphosphokinase


organism: [Bacillus] caldolyticus


enzyme_display_name: thiamine diphosphokinase

organism: Glycine max

substrate_name: thiamine

assay_conditions_summary: pH 8.1; 310.15 K; cofactor ATP

enzyme: thiamine diphosphokinase

substrate: thiamine


substrate_name: GTP

substrate: GTP


substrate_name: CTP

substrate: CTP


substrate_name: UTP

substrate: UTP


organism: Paracoccus denitrificans


assay_conditions_summary: pH 9; 310.15 K; cofactor ATP


enzyme_display_name: pyruvate, water dikinase

organism: Pyrococcus horikoshii

enzyme: pyruvate, water dikinase


substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


substrate_name: phosphate

substrate: phosphate


enzyme_display_name: glutaconate CoA-transferase

organism: Acidaminococcus fermentans

substrate_name: glutaryl-CoA

assay_conditions_summary: pH 7; 298.15 K; cofactor CoA

proof_lines: pH 7, cosubstrate acetate, 65 kDa GctF fusion protein

enzyme: glutaconate CoA-transferase

substrate: glutaryl-CoA


assay_conditions_summary: pH 7; cofactor CoA

proof_lines: pH 7, cosubstrate acetate, wild-type enzyme


assay_conditions_summary: pH 7; 310.15 K; cofactor CoA


organism: Pleurotus eryngii

substrate_name: benzyl glucuronic acid

substrate: benzyl glucuronic acid


organism: Punctularia strigosozonata

substrate_name: lignin-rich pellet

substrate: lignin-rich pellet


organism: Schizophyllum commune


organism: Thermothelomyces thermophilus

proof_lines:
pH 6.0, 50°C
pH 7.0, 50°C, recombinant enzyme
pH 6.0, 45°C


enzyme_display_name: feruloyl esterase

organism: Lentilactobacillus buchneri

substrate_name: 4-nitrophenyl ferulate

enzyme: feruloyl esterase

substrate: 4-nitrophenyl ferulate


substrate_name: methyl ferulate

substrate: methyl ferulate


organism: Sorangium cellulosum

substrate_name: methyl sinapate

assay_conditions_summary: pH 7

proof_lines: pH 7.0, temperature not specified in the publication

substrate: methyl sinapate


organism: Talaromyces funiculosus


substrate_name: methyl caffeate

substrate: methyl caffeate


substrate_name: methyl coumarate

substrate: methyl coumarate


organism: Thermothielavioides terrestris


enzyme_display_name: cutinase

substrate_name: 4-nitrophenyl propionate

enzyme: cutinase

substrate: 4-nitrophenyl propionate


substrate_name: 4-nitrophenyl pentanoate

substrate: 4-nitrophenyl pentanoate


substrate_name: 4-nitrophenyl butanoate

substrate: 4-nitrophenyl butanoate


enzyme_display_name: acyloxyacyl hydrolase

organism: Homo sapiens

substrate_name: long chain lipopolysaccharide

enzyme: acyloxyacyl hydrolase

substrate: long chain lipopolysaccharide


enzyme_display_name: pyrethroid hydrolase

organism: Mus musculus

enzyme: pyrethroid hydrolase


substrate_name: cypermethrin

substrate: cypermethrin


substrate_name: 4-nitrophenyl acetate

substrate: 4-nitrophenyl acetate


enzyme_display_name: exodeoxyribonuclease I

substrate_name: 3'-end 6-fluorescein-labeled poly(dT)10

enzyme: exodeoxyribonuclease I

substrate: 3'-end 6-fluorescein-labeled poly(dT)10


enzyme_display_name: acyl-CoA hydrolase

organism: Streptomyces coelicolor

substrate_name: N-acetylcysteamine propionate

assay_conditions_summary: pH 7.5; 303.15 K; cofactor CoA

enzyme: acyl-CoA hydrolase

substrate: N-acetylcysteamine propionate


organism: Umbellularia californica

substrate_name: decanoyl-[acyl-carrier protein]

proof_lines: fully reversible by dialysis or dilution

substrate: decanoyl-[acyl-carrier protein]


substrate_name: dodecanoyl-[acyl-carrier protein]

smiles: CCCCCCCCCCCC(=O)S

substrate: dodecanoyl-[acyl-carrier protein]


substrate_name: tetradecanoyl-[acyl-carrier protein]

substrate: tetradecanoyl-[acyl-carrier protein]


substrate_name: octadecenoyl-[acyl-carrier protein]

smiles: CCCCCCCCC=CCCCCCCCC(=O)S

substrate: octadecenoyl-[acyl-carrier protein]


organism: Schizosaccharomyces pombe

substrate_name: DNA junction J1T2

substrate: DNA junction J1T2


enzyme_display_name: 2-lysophosphatidate phosphatase

organism: Saccharomyces cerevisiae

enzyme: 2-lysophosphatidate phosphatase


enzyme_display_name: protein-tyrosine-phosphatase

organism: Shewanella sp.

substrate_name: 4-nitrophenyl phosphate

proof_lines: wild type enzyme, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C

enzyme: protein-tyrosine-phosphatase

substrate: 4-nitrophenyl phosphate


proof_lines: mutant enzyme I307A, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C


proof_lines: mutant enzyme I307F, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C


proof_lines: mutant enzyme I307L, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C


proof_lines: mutant enzyme I307M, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C


organism: Synechocystis sp.

substrate_name: casein

proof_lines: isoform SynPPM3, activity against phosphoserine residues, pH 7.5, 37°C

proof_lines: isoform SynPPM3, activity against phosphotyrosine residues, pH 7.5, 37°C

proof_lines: isoform SynPPP1, activity against phosphoserine residues, pH 7.5, 37°C

substrate: casein


organism: Nicotiana tabacum

substrate_name: 2-carboxyarabinitol 1-phosphate

substrate: 2-carboxyarabinitol 1-phosphate


enzyme_display_name: phosphodiesterase I

enzyme: phosphodiesterase I


enzyme_display_name: aryldialkylphosphatase

substrate_name: 4-chlorophenyl diethyl phosphate

assay_conditions_summary: pH 8

enzyme: aryldialkylphosphatase

substrate: 4-chlorophenyl diethyl phosphate


substrate_name: 3-fluorophenyl diethyl phosphate

assay_conditions_summary: pH 8; 298.15 K; buffer 50 mM Tris-HCl

proof_lines: in 50 mM Tris-HCl buffer, 10 mM CaCl2, pH 7.4

proof_lines: pH 10.5, 25°C, recombinant His6-tagged enzyme

substrate: 3-fluorophenyl diethyl phosphate


substrate_name: 3-cyanophenyl diethyl phosphate

substrate: 3-cyanophenyl diethyl phosphate


substrate_name: 2,4-dinitrophenyl diethyl phosphate

substrate: 2,4-dinitrophenyl diethyl phosphate


substrate_name: 4-cyanophenyl diethyl phosphate

substrate: 4-cyanophenyl diethyl phosphate


substrate_name: paraoxon

proof_lines: pH 8.0, recombinant genetic variant G1A7

proof_lines: pH 8.0, recombinant genetic variant G1B11

proof_lines: pH 8.0, recombinant genetic variant G2C2

substrate: paraoxon


substrate_name: chlorpyrifos

proof_lines: recombinant wild type enzyme, in 0.1 M Tris-HCl (pH 8.5), 2 M NaCl, and 2 mM CaCl2

substrate: chlorpyrifos


substrate_name: diazoxon

substrate: diazoxon


proof_lines:
wild-type, pH 8.0, 25°C
pH 8.0, wild-type enzyme
wild-type, pH 10.5, 25°C


proof_lines:
mutant H115W, pH 8.0, 25°C
mutant H115W, pH 10.5, 25°C
pH 10.5, 25°C, mutant enzyme H115W


substrate_name: cyclosarin

assay_conditions_summary: pH 7.4; buffer 50 mM Tris-HCl

substrate: cyclosarin


substrate_name: parathion

proof_lines: pH 8.0, 25°C, enzyme administered in rats in vivo

substrate: parathion


proof_lines: pH 10.5, 25°C, mutant enzyme H115W/N133S


proof_lines: mutant G11A and mutant G11S, pH 10.5, 25°C


substrate_name: phenyl acetate

proof_lines: pH 8.5, 25°C, wild-type enzyme, substrate paraoxon

proof_lines: pH 10.5, 25°C, wild-type enzyme, substrate paraoxon

substrate: phenyl acetate


substrate_name: diisopropyl fluorophosphate

smiles: CC(C)OP(=O)(OC(C)C)F

substrate: diisopropyl fluorophosphate


proof_lines: pH 10.5, 25°C, mutant enzyme H115W, substrate paraoxon


proof_lines: pH 8.0, recombinant genetic variant G1C4

proof_lines: pH 8.0, recombinant genetic variant G2D6

proof_lines: pH 8.0, recombinant genetic variant G3C9


smiles: CCOP(=O)(C)SCCN(C(C)C)C(C)C

proof_lines: wild type enzyme, in 20 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C


organism: Oryctolagus cuniculus

assay_conditions_summary: pH 8; buffer 20 mM Tris-HCl

proof_lines: in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication


substrate_name: diethyl paraoxon

proof_lines: pH 10.5, 25°C, recombinant mutant H115W/T332S

substrate: diethyl paraoxon


proof_lines: pH 10.5, 25°C, recombinant wild-type enzyme


organism: Rattus norvegicus

substrate_name: Tabun

smiles: CCOP(=O)(C#N)N(C)C

substrate: Tabun


proof_lines:
37°C, pH 7.4
pH 8.5, 37°C, enzyme form M2
37°C, pH 8.5


organism: Streptococcus pneumoniae

substrate_name: blood group A glycoconjugate

substrate: blood group A glycoconjugate


enzyme_display_name: alpha-agarase

organism: Thalassomonas sp. LD5

substrate_name: agarose

enzyme: alpha-agarase

substrate: agarose


enzyme_display_name: alpha,alpha-phosphotrehalase

organism: Paenibacillus popilliae

substrate_name: alpha,alpha-trehalose 6-phosphate

enzyme: alpha,alpha-phosphotrehalase

substrate: alpha,alpha-trehalose 6-phosphate


organism: Gallus gallus


organism: Lathyrus oleraceus


enzyme_display_name: endo-alpha-N-acetylgalactosaminidase

organism: Bifidobacterium longum

proof_lines:
mutant enzyme E822A
mutant E822A, pH 5.0, 37°C

enzyme: endo-alpha-N-acetylgalactosaminidase


proof_lines:
mutant enzyme D762A
mutant D762A, pH 5.0, 37°C


proof_lines: pH 4.5, temperature not specified in the publication, wild-type enzyme, Neu5Acalpha2-3Galbeta1-3GalNAc release from asialo-fetuin


substrate_name: asialo-casein glycomacropeptide

proof_lines:
37°C, pH 4.5, mutant enzyme H685A/H718A
37°C, pH 5.0, mutant enzyme H685A/H718A
37°C, pH 6.0, mutant enzyme H685A/H718A

substrate: asialo-casein glycomacropeptide


proof_lines:
37°C, pH 4.5, mutant enzyme H685Q/H718Q
37°C, pH 6.0, mutant enzyme H685Q/H718Q
37°C, pH 5.0, mutant enzyme H685Q/H718Q


substrate_name: Neu5Acalpha2-3Galbeta1-3GalNAc O-linked glycan of asialo-fetuin

proof_lines: pH 4.5, temperature not specified in the publication, mutant enzyme E1294K


proof_lines: pH 4.5, temperature not specified in the publication, mutant enzyme E1294K/K1199A

substrate: Neu5Acalpha2-3Galbeta1-3GalNAc O-linked glycan of asialo-fetuin


proof_lines: pH 4.5, temperature not specified in the publication, mutant enzyme E1294A


proof_lines: pH 4.5, temperature not specified in the publication, mutant enzyme E1294H


proof_lines: pH 4.5, temperature not specified in the publication, mutant enzyme E1294K/Q894A


proof_lines: pH 4.5, temperature not specified in the publication, mutant enzyme E1294M


organism: Enterococcus faecalis


enzyme_display_name: aminodeoxyfutalosine nucleosidase

organism: Campylobacter jejuni

enzyme: aminodeoxyfutalosine nucleosidase


enzyme_display_name: leukotriene-A4 hydrolase

organism: Cavia porcellus

substrate_name: leukotriene A4

enzyme: leukotriene-A4 hydrolase

substrate: leukotriene A4


enzyme_display_name: methionyl aminopeptidase

organism: Encephalitozoon cuniculi

substrate_name: Met-Ala-Ser

smiles: CC(C(=O)NC(CO)C(=O)O)NC(=O)C(CCSC)N

enzyme: methionyl aminopeptidase

substrate: Met-Ala-Ser


organism: Leishmania donovani

proof_lines:
37°C, pH 7.4, in presence of MgCl2
37°C, pH 7.4, in presence of CaCl2
37°C, pH 7.4, in presence of FeCl2


enzyme_display_name: aspartyl aminopeptidase

organism: Trichinella spiralis

proof_lines: 37°C, pH not specified in the publication

enzyme: aspartyl aminopeptidase


enzyme_display_name: aminopeptidase S

organism: Streptomyces griseus

proof_lines: pH 8.0, 37°C, recombinant mutant E196A, in presence of Ca2+

proof_lines: pH 8.0, 37°C, recombinant mutant E196A, in absence of Ca2+

enzyme: aminopeptidase S


proof_lines: pH 8.0, 37°C, recombinant wild-type enzyme, in presence of Ca2+

proof_lines: pH 8.0, 37°C, recombinant wild-type enzyme, in absence of Ca2+


proof_lines: pH 8.0, 37°C, recombinant mutant D3A/D262G, in presence of Ca2+

proof_lines: pH 8.0, 37°C, recombinant mutant D3A/D262G, in absence of Ca2+


proof_lines: pH 8.0, 37°C, recombinant chimeric mutant, in presence of Ca2+

proof_lines: pH 8.0, 37°C, recombinant chimeric mutant, in absence of Ca2+


proof_lines:
pH 8.0, 30°C, in absence of Ca2+
pH 8.0, 30°C, in presence of Ca2+
pH 8.0, 30°C


proof_lines: pH 8.0, 22°C, acetylated aminopeptidases 2


substrate_name: bis(4-nitrophenyl) phosphate

proof_lines: in presence of Mn-Ni-hetero-dinuclear aminopeptidase

proof_lines: in presence of Ni-Ni-homo-dinuclear aminopeptidase

proof_lines: in presence of Mn-Cd-hetero-dinuclear aminopeptidase

substrate: bis(4-nitrophenyl) phosphate


proof_lines:
pH 8.0, 30°C, in absence of Ca2+
pH 8.0, 30°C, in presence of Ca2+


enzyme_display_name: cytosol non-specific dipeptidase

organism: Mus musculus

substrate_name: Ala-Leu

smiles: CC(C)CC(C(=O)O)NC(=O)C(C)N

enzyme: cytosol non-specific dipeptidase

substrate: Ala-Leu


substrate_name: Leu-Leu

smiles: CC(C)CC(C(=O)NC(CC(C)C)C(=O)O)N

substrate: Leu-Leu


substrate_name: Leu-Gly

smiles: CC(C)CC(C(=O)NCC(=O)O)N

substrate: Leu-Gly


substrate_name: Gly-L-Asn

smiles: C(C(C(=O)O)NC(=O)CN)C(=O)N

substrate: Gly-L-Asn


substrate_name: Ala-Gly

smiles: CC(C(=O)NCC(=O)O)N

substrate: Ala-Gly


substrate_name: Ala-Ile

smiles: CCC(C)C(C(=O)O)NC(=O)C(C)N

substrate: Ala-Ile


substrate_name: Gly-L-Ala

smiles: CC(C(=O)O)NC(=O)CN

substrate: Gly-L-Ala


enzyme_display_name: Xaa-Xaa-Pro tripeptidyl-peptidase

organism: Porphyromonas gingivalis

assay_conditions_summary: pH 7; 310.15 K; buffer 20 mM Tris-HCl

proof_lines: in 20 mM Tris-HCl buffer (pH 7.0), at 37°C

enzyme: Xaa-Xaa-Pro tripeptidyl-peptidase


proof_lines: mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0), at 37°C

proof_lines: mutant enzyme E636A, using Gly-Ala-Pro-2-naphtylamide as a substrate, in 20 mM Tris-HCl (pH 7.0), at 37°C


substrate_name: Gly-Ala-Pro-beta-naphthylamide

mutation_signature: prolyl tripeptidyl aminopeptidase with an N-terminal truncation of 38 residues prolyl tripeptidyl aminopeptidase with an N-terminal truncation of 38 residues

mutation_type: truncation

substrate: Gly-Ala-Pro-beta-naphthylamide


proof_lines: wild type enzyme, in 20 mM Tris-HCl (pH 7.0), at 37°C

proof_lines: wild type enzyme, using Gly-Ala-Pro-2-naphtylamide as a substrate, in 20 mM Tris-HCl (pH 7.0), at 37°C


enzyme_display_name: carboxypeptidase C

organism: Homo sapiens

substrate_name: CBZ-Phe-Leu

enzyme: carboxypeptidase C

substrate: CBZ-Phe-Leu


organism: Kluyveromyces marxianus

substrate_name: N-benzoyl-L-tyrosine-p-nitroanilide

substrate: N-benzoyl-L-tyrosine-p-nitroanilide


enzyme_display_name: carboxypeptidase A

enzyme: carboxypeptidase A


substrate_name: hippuryl-L-phenylalanine

substrate: hippuryl-L-phenylalanine


substrate_name: p-nitrophenyl acetate

substrate: p-nitrophenyl acetate


substrate_name: ochratoxin A

substrate: ochratoxin A


substrate_name: angiotensin I

substrate: angiotensin I


organism: Struthio camelus

substrate_name: hippuryl-DL-phenyllactate

substrate: hippuryl-DL-phenyllactate


substrate_name: Carbobenzoxy-Gly-Phe

substrate: Carbobenzoxy-Gly-Phe


enzyme_display_name: carboxypeptidase B

substrate_name: hippuryl-Arg

proof_lines:
carboxypeptidase B I
carboxypeptidase B II

enzyme: carboxypeptidase B

substrate: hippuryl-Arg


substrate_name: hippuryl-L-Arg

proof_lines:
carboxypeptidase B I
carboxypeptidase B II
carboxypeptidase B

substrate: hippuryl-L-Arg


substrate_name: hippuryl-Asp

substrate: hippuryl-Asp


enzyme_display_name: pancreatic endopeptidase E

enzyme: pancreatic endopeptidase E


enzyme_display_name: cathepsin B

organism: Larimichthys crocea

substrate_name: Z-L-Phe-L-Arg 7-amido-4-methylcoumarin

proof_lines: pH and temperature not specified in the publication

enzyme: cathepsin B

substrate: Z-L-Phe-L-Arg 7-amido-4-methylcoumarin


enzyme_display_name: SARS coronavirus main proteinase

organism: Severe acute respiratory syndrome coronavirus 2

substrate_name: Dabcyl-KTSAVLQSGFRKME-EDANS

enzyme: SARS coronavirus main proteinase

substrate: Dabcyl-KTSAVLQSGFRKME-EDANS


substrate_name: DABCYL-TSAVLQSGFRKME-EDANS

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: DABCYL-TSAVLQSGFRKME-EDANS


enzyme_display_name: aspergillopepsin I

organism: Aspergillus phoenicis

substrate_name: Trypsinogen

enzyme: aspergillopepsin I

substrate: Trypsinogen


enzyme_display_name: gelatinase A

organism: Gallus gallus

substrate_name: type I collagen

enzyme: gelatinase A

substrate: type I collagen


enzyme_display_name: vibriolysin

organism: Pseudoalteromonas sp. SM495

enzyme: vibriolysin


organism: Pseudoalteromonas sp. SM9913


enzyme_display_name: choriolysin H

organism: Oryzias latipes

substrate_name: NPQVPQYPSKPQ

enzyme: choriolysin H

substrate: NPQVPQYPSKPQ


proof_lines:
pH 7.5, 37°C, in presence of IGF-I
pH 7.5, 37°C, in presence of IGF-II
pH 7.5, 37°C, in absence of IGF


enzyme_display_name: protein deglycase

organism: Vibrio cholerae

substrate_name: methylglyoxal

smiles: CC(=O)C=O

enzyme: protein deglycase

substrate: methylglyoxal


enzyme_display_name: blasticidin-S deaminase

organism: Aspergillus terreus

substrate_name: blasticidin S

mutation_type: deletion

enzyme: blasticidin-S deaminase

substrate: blasticidin S


proof_lines: deletion mutants DELTA128-130, DELTA127-130, DELTA126-130, 30°C


enzyme_display_name: aminopyrimidine aminohydrolase

organism: Aneurinibacillus aneurinilyticus

substrate_name: thiamine

enzyme: aminopyrimidine aminohydrolase

substrate: thiamine


enzyme_display_name: glucosamine-6-phosphate deaminase

organism: Drosophila virilis

substrate_name: D-fructose 6-phosphate

enzyme: glucosamine-6-phosphate deaminase

substrate: D-fructose 6-phosphate


organism: Proteus vulgaris

substrate_name: D-glucosamine 6-phosphate

substrate: D-glucosamine 6-phosphate


organism: Penicillium citrinum


enzyme_display_name: exopolyphosphatase

organism: Saccharomyces cerevisiae

enzyme: exopolyphosphatase


substrate_name: adenosine 5'-tetraphosphate

proof_lines: at pH 4.8, 50 mM sodium acetate, 5 mM CoCl2

substrate: adenosine 5'-tetraphosphate


substrate_name: Polyphosphate

proof_lines: pH 7.2, 25°C, recombinant wild-type enzyme

substrate: Polyphosphate


substrate_name: (Phosphate)n

proof_lines:
37°C, recombinant enzyme
n = 208, pH 7.2, 30°C
n = 208

substrate: (Phosphate)n


substrate_name: cAMP

substrate: cAMP


proof_lines: polyphosphate 208, in the presence of 2.5 mM Mg2+


organism: Trypanosoma cruzi

substrate_name: guanosine 5'-tetraphosphate

substrate: guanosine 5'-tetraphosphate


substrate_name: Triphosphate

substrate: Triphosphate


organism: Ascaris suum

proof_lines: C-terminally tagged protein, pH 7.2, 20°C

proof_lines: N-terminally tagged protein, pH 7.2, 20°C


enzyme_display_name: diadenosine hexaphosphate hydrolase (AMP-forming)

organism: Homo sapiens

proof_lines:
pH 7.6, 37°C, hApsI-39N
pH 7.6, 37°C, hApsI
pH 7.6, 37°C, hAps2


proof_lines:
pH 7.6, 37°C, hApsI
pH 7.6, 37°C, hApsI-39N
pH 7.6, 37°C

enzyme: diadenosine hexaphosphate hydrolase (AMP-forming)


substrate_name: Unknown

substrate: Unknown


enzyme_display_name: nucleotide diphosphatase

organism: Columba livia

substrate_name: NADH

assay_conditions_summary: cofactor NADH

enzyme: nucleotide diphosphatase

substrate: NADH


organism: Haemophilus influenzae

substrate_name: pyridine adenine dinucleotide

substrate: pyridine adenine dinucleotide


substrate_name: NAD+

assay_conditions_summary: cofactor NAD+

proof_lines: biphasic hydrolysis, functions in a manner indicative of negative cooperativity

substrate: NAD+


substrate_name: 3-acetylpyridine adenine dinucleotide

substrate: 3-acetylpyridine adenine dinucleotide


substrate_name: nicotinic acid adenine dinucleotide

substrate: nicotinic acid adenine dinucleotide


substrate_name: nicotinamide hypoxanthine dinucleotide

substrate: nicotinamide hypoxanthine dinucleotide


substrate_name: FAD

assay_conditions_summary: cofactor FAD

substrate: FAD


substrate_name: 3-Aminopyridine adenine dinucleotide

substrate: 3-Aminopyridine adenine dinucleotide


organism: Thermotoga maritima

substrate_name: CTP

substrate: CTP


substrate_name: UTP

substrate: UTP


substrate_name: ATP

assay_conditions_summary: pH 8; 343.15 K; cofactor ATP

substrate: ATP


substrate_name: dTTP

substrate: dTTP


substrate_name: GTP

substrate: GTP


substrate_name: dGTP

substrate: dGTP


substrate_name: dCTP

substrate: dCTP


substrate_name: dATP

substrate: dATP


enzyme_display_name: 2,4-diacetylphloroglucinol hydrolase

organism: Pseudomonas fluorescens

enzyme: 2,4-diacetylphloroglucinol hydrolase


organism: Burkholderia cepacia


organism: Sphingobium sp. SYK-6


enzyme_display_name: haloalkane dehalogenase

organism: Plesiocystis pacifica

smiles: C(CBr)Br

enzyme: haloalkane dehalogenase


smiles: CCCCCCCBr


smiles: CC(CBr)Br


smiles: CCCCCCBr


smiles: CCCBr


smiles: CCCCBr


smiles: CCCCCBr


organism: Sphingomonas paucimobilis

smiles: CC(CCl)Cl


smiles: C(CCl)Cl


smiles: CCCCCl

proof_lines: pH 8.6, 37°C, recombinant wild-type enzyme, data from 2 experimental sets


enzyme_display_name: pyruvate decarboxylase

organism: Zymobacter palmae

substrate_name: pyruvate

assay_conditions_summary: pH 6.5; buffer pH 6

proof_lines: in 50 mM potassium phosphate buffer pH 6.5, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate

enzyme: pyruvate decarboxylase

substrate: pyruvate


enzyme_display_name: acetoacetate decarboxylase

organism: Clostridium acetobutylicum

substrate_name: acetoacetate

enzyme: acetoacetate decarboxylase

substrate: acetoacetate


assay_conditions_summary: pH 5.95; 298.15 K; buffer note

proof_lines: at 25°C, in 50 mM phosphate buffer, pH 5.95


assay_conditions_summary: pH 5.95; 298.15 K; buffer 50 mM phosphate


organism: Paenibacillus polymyxa


enzyme_display_name: acetolactate decarboxylase

organism: Brevibacillus brevis

assay_conditions_summary: pH 6

proof_lines: pH 6.0, temperature not specified in the publication

enzyme: acetolactate decarboxylase


organism: Leuconostoc lactis


enzyme_display_name: cis-aconitate decarboxylase

organism: Mus musculus

substrate_name: cis-aconitate

proof_lines: pH 6.5, 37°C, recombinant wild-type enzyme

proof_lines: wild-type, pH 6.5, 37°C, 167 mM phosphate buffer

enzyme: cis-aconitate decarboxylase

substrate: cis-aconitate


enzyme_display_name: oxalyl-CoA decarboxylase

organism: Cupriavidus oxalaticus

substrate_name: oxalyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: oxalyl-CoA decarboxylase

substrate: oxalyl-CoA


organism: Spinacia oleracea

substrate_name: H+; Acetoacetyl-[acp]; NADPH

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

assay_conditions_summary: cofactor NADPH

substrate: H+; Acetoacetyl-[acp]; NADPH


enzyme_display_name: L-threonine 3-dehydrogenase

organism: Gallus gallus

substrate_name: NAD+; L-Threonine

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

enzyme: L-threonine 3-dehydrogenase

substrate: NAD+; L-Threonine


enzyme_display_name: D-threo-aldose 1-dehydrogenase

organism: Sus scrofa

substrate_name: L-Fucose; NAD+

enzyme: D-threo-aldose 1-dehydrogenase

substrate: L-Fucose; NAD+


organism: Cavia porcellus

substrate_name: Testosterone; NAD+

substrate: Testosterone; NAD+


organism: Mesocricetus auratus

substrate_name: NAD+; Testosterone

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NAD+

substrate: NAD+; Testosterone


organism: Haemophilus influenzae

substrate_name: D-Ribulose 5-phosphate; H+; NADPH

substrate: D-Ribulose 5-phosphate; H+; NADPH


enzyme_display_name: glucose 1-dehydrogenase [NAD(P)(+)]

substrate_name: beta-D-Glucose; NAD+

enzyme: glucose 1-dehydrogenase [NAD(P)(+)]

substrate: beta-D-Glucose; NAD+


enzyme_display_name: ferroxidase

organism: Desulfovibrio sp. A2

enzyme: ferroxidase


substrate_name: NAD+; D-Glyceraldehyde 3-phosphate; Phosphate


enzyme_display_name: aldehyde dehydrogenase (NADP(+))

organism: Saccharomyces cerevisiae

substrate_name: Acetaldehyde; NADP+; H2O

assay_conditions_summary: cofactor NADP+

enzyme: aldehyde dehydrogenase (NADP(+))

substrate: Acetaldehyde; NADP+; H2O


enzyme_display_name: long-chain-aldehyde dehydrogenase

organism: Homo sapiens

substrate_name: Acetaldehyde; H2O; NAD+

enzyme: long-chain-aldehyde dehydrogenase

substrate: Acetaldehyde; H2O; NAD+


enzyme_display_name: aldehyde dehydrogenase [NAD(P)(+)]

substrate_name: H2O; Benzaldehyde; NAD+

enzyme: aldehyde dehydrogenase [NAD(P)(+)]

substrate: H2O; Benzaldehyde; NAD+


substrate_name: NADP+; Benzene dihydrodiol

substrate: NADP+; Benzene dihydrodiol


organism: Rattus norvegicus

substrate_name: NADPH; H+; p-Nitrobenzaldehyde

substrate: NADPH; H+; p-Nitrobenzaldehyde


enzyme_display_name: glutamate dehydrogenase

organism: Laccaria bicolor

substrate_name: NADH; NH3; H+; 2-Oxoglutarate

enzyme: glutamate dehydrogenase

substrate: NADH; NH3; H+; 2-Oxoglutarate


enzyme_display_name: dihydrofolate reductase

organism: Leishmania tropica

substrate_name: Dihydrofolate; NADPH; H+

enzyme: dihydrofolate reductase

substrate: Dihydrofolate; NADPH; H+


enzyme_display_name: pteridine reductase

organism: Leishmania donovani

substrate_name: Biopterin; NADPH; H+

assay_conditions_summary: pH 4.8; 303.15 K; cofactor NADPH

proof_lines: pH and temperature not specified in the publication

enzyme: pteridine reductase

substrate: Biopterin; NADPH; H+


substrate_name: 2-Oxoglutarate; 4-Aminobutanoate

substrate: 2-Oxoglutarate; 4-Aminobutanoate


enzyme_display_name: hexokinase

organism: Ustilago maydis

substrate_name: ATP; Glucose

assay_conditions_summary: cofactor ATP

enzyme: hexokinase

substrate: ATP; Glucose


organism: Crassostrea virginica

substrate_name: D-Fructose 6-phosphate; ATP

substrate: D-Fructose 6-phosphate; ATP


enzyme_display_name: glucokinase

substrate_name: ATP; alpha-D-Glucose

enzyme: glucokinase

substrate: ATP; alpha-D-Glucose


enzyme_display_name: fructokinase

organism: Saccharum officinarum

substrate_name: ATP; D-Fructose

enzyme: fructokinase

substrate: ATP; D-Fructose


enzyme_display_name: microsomal epoxide hydrolase

substrate_name: H2O; (1-Chloroethenyl)oxirane

enzyme: microsomal epoxide hydrolase

substrate: H2O; (1-Chloroethenyl)oxirane


enzyme_display_name: mannitol dehydrogenase

organism: Apium graveolens

substrate_name: NADH

enzyme: mannitol dehydrogenase

substrate: NADH


substrate_name: D-mannose

substrate: D-mannose


enzyme_display_name: L-2-hydroxyglutarate dehydrogenase

organism: Alcaligenes sp.

enzyme: L-2-hydroxyglutarate dehydrogenase


smiles: C(CC(=O)O)C(C(=O)O)O


substrate_name: NAD+

substrate: NAD+


enzyme_display_name: proline 3-hydroxylase

organism: Streptomyces sp.

substrate_name: L-2-azetidinecarboxylic acid

enzyme: proline 3-hydroxylase

substrate: L-2-azetidinecarboxylic acid


substrate_name: L-pipecolic acid

substrate: L-pipecolic acid


enzyme_display_name: (S)-stylopine synthase

organism: Eschscholzia californica

substrate_name: (S)-cheilanthifoline

enzyme: (S)-stylopine synthase

substrate: (S)-cheilanthifoline


organism: Agaricus bisporus


organism: Acinetobacter sp. HBS-2

substrate_name: propionaldehyde

smiles: CCC=O

substrate: propionaldehyde


substrate_name: formaldehyde

smiles: C=O

substrate: formaldehyde


substrate_name: acetaldehyde

smiles: CC=O

substrate: acetaldehyde


enzyme_display_name: formaldehyde dehydrogenase

organism: Arthrobacter sp.

enzyme: formaldehyde dehydrogenase


organism: Pseudomonas putida

smiles: C(CC=O)CN=C(N)N


enzyme_display_name: prephenate dehydrogenase

organism: Pseudomonas flexibilis

substrate_name: prephenate

enzyme: prephenate dehydrogenase

substrate: prephenate


enzyme_display_name: dihydroorotate dehydrogenase (NAD(+))

organism: Faecalicatena orotica

substrate_name: Unknown

enzyme: dihydroorotate dehydrogenase (NAD(+))

substrate: Unknown


enzyme_display_name: protochlorophyllide reductase

organism: Tetradesmus obliquus

substrate_name: protochlorophyllide

enzyme: protochlorophyllide reductase

substrate: protochlorophyllide


substrate_name: NADPH

substrate: NADPH


enzyme_display_name: acyl-CoA oxidase

organism: Spinacia oleracea

substrate_name: myristoyl-CoA

enzyme: acyl-CoA oxidase

substrate: myristoyl-CoA


substrate_name: linoleoyl-CoA

substrate: linoleoyl-CoA


substrate_name: stearoyl-CoA

substrate: stearoyl-CoA


substrate_name: butyryl-CoA

substrate: butyryl-CoA


enzyme_display_name: L-amino-acid oxidase

organism: Gallus gallus

substrate_name: L-leucine

smiles: CC(C)CC(C(=O)O)N

enzyme: L-amino-acid oxidase

substrate: L-leucine


enzyme_display_name: glutamate synthase (ferredoxin)

substrate_name: reduced ferredoxin

enzyme: glutamate synthase (ferredoxin)

substrate: reduced ferredoxin


enzyme_display_name: pyrroline-5-carboxylate reductase

organism: Acetoanaerobium sticklandii

substrate_name: NADH

enzyme: pyrroline-5-carboxylate reductase

substrate: NADH


organism: Cucurbita moschata


enzyme_display_name: saccharopine dehydrogenase (NAD(+), L-lysine-forming)

organism: Phycomyces blakesleeanus

substrate_name: L-lysine

smiles: C(CCN)CC(C(=O)O)N

substrate: L-lysine


enzyme_display_name: nitrate reductase (NADPH)

organism: Neurospora crassa

enzyme: nitrate reductase (NADPH)


enzyme_display_name: nitrite reductase (NO-forming)

organism: Cereibacter sphaeroides

substrate_name: nitrite

enzyme: nitrite reductase (NO-forming)

substrate: nitrite


enzyme_display_name: adenylyl-sulfate reductase

organism: Chlorobium limicola

substrate_name: ferricyanide

enzyme: adenylyl-sulfate reductase

substrate: ferricyanide


substrate_name: AMP

substrate: AMP


substrate_name: sulfite

substrate: sulfite


organism: Desulfobulbus propionicus

substrate_name: cytochrome c

substrate: cytochrome c


organism: Leishmania donovani

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


substrate_name: N-acetyl-S-(E,E)-farnesyl-L-cysteine

substrate: N-acetyl-S-(E,E)-farnesyl-L-cysteine


substrate_name: N-acetyl-S-geranylgeranyl-L-cysteine

substrate: N-acetyl-S-geranylgeranyl-L-cysteine


enzyme_display_name: ATP citrate synthase

organism: Lipomyces starkeyi

substrate_name: citrate

enzyme: ATP citrate synthase

substrate: citrate


substrate_name: ATP

substrate: ATP


organism: Nicotiana tabacum

substrate_name: 2-carboxyarabinitol 1,5-bisphosphate

substrate: 2-carboxyarabinitol 1,5-bisphosphate


enzyme_display_name: blasticidin-S deaminase

organism: Aspergillus terreus

substrate_name: pyrimidinoblasticidin S

enzyme: blasticidin-S deaminase

substrate: pyrimidinoblasticidin S


enzyme_display_name: haloacetate dehalogenase

organism: Moraxella sp.

substrate_name: monoiodoacetate

smiles: C(C(=O)O)I

enzyme: haloacetate dehalogenase

substrate: monoiodoacetate


enzyme_display_name: acetoacetate decarboxylase

organism: Clostridium acetobutylicum

substrate_name: 3-oxo-3-phenylpropionic acid

enzyme: acetoacetate decarboxylase

substrate: 3-oxo-3-phenylpropionic acid


enzyme_display_name: acetolactate decarboxylase

organism: Brevibacillus brevis

enzyme: acetolactate decarboxylase


organism: Lacticaseibacillus casei


organism: Synechococcus elongatus PCC 7942 = FACHB-805

substrate_name: ethylbenzoylacetate

assay_conditions_summary: pH 7; buffer 0.1 M sodium phosphate; cofactor NADPH

proof_lines: with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0

substrate: ethylbenzoylacetate


substrate_name: alpha-chloroacetophenone

substrate: alpha-chloroacetophenone


substrate_name: propiophenone

substrate: propiophenone


substrate_name: ethyl 4-chloroacetoacetate

smiles: CCOC(=O)CC(=O)CCl

substrate: ethyl 4-chloroacetoacetate


substrate_name: acetophenone

substrate: acetophenone


smiles: CC(=O)C(F)(F)F


organism: Xanthomonas campestris pv. campestris

substrate_name: 3-oxododecanoyl-[acyl-carrier protein]

substrate: 3-oxododecanoyl-[acyl-carrier protein]


enzyme_display_name: carnitine 3-dehydrogenase

organism: Rhizobium sp.

substrate_name: NAD+

assay_conditions_summary: pH 8.5; 303.15 K; cofactor NAD+

proof_lines: mutant lacking 162 C-terminal residues, pH 8.5, 30°C

enzyme: carnitine 3-dehydrogenase

substrate: NAD+


substrate_name: carnitine

substrate: carnitine


proof_lines: mutant enzyme S220G/F221A, at pH 9.5 and 30°C


proof_lines:
wild type enzyme, at pH 9.5 and 30°C
wild-type, pH 8.5, 30°C


assay_conditions_summary: pH 9.5; 303.15 K; cofactor NAD+


enzyme_display_name: dTDP-4-dehydrorhamnose reductase

organism: Streptococcus sp.

substrate_name: dTDP-alpha-D-glucose

proof_lines: at pH 7.5, temperature not specified in the publication

enzyme: dTDP-4-dehydrorhamnose reductase

substrate: dTDP-alpha-D-glucose


organism: Gallus gallus

substrate_name: prostaglandin E2

substrate: prostaglandin E2


substrate_name: prostaglandin F2alpha

substrate: prostaglandin F2alpha


substrate_name: Unknown

substrate: Unknown


organism: Rattus norvegicus

substrate_name: dehydroepiandrosterone

substrate: dehydroepiandrosterone


substrate_name: pregnenolone

substrate: pregnenolone


assay_conditions_summary: pH 7.25; 310.15 K; cofactor NAD+


substrate_name: NADP+

substrate: NADP+


enzyme_display_name: 11beta-hydroxysteroid dehydrogenase

organism: Homo sapiens

substrate_name: corticosterone

proof_lines: 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1

enzyme: 11beta-hydroxysteroid dehydrogenase

substrate: corticosterone


proof_lines: isoform 11betaHSD2, pH and temperature not specified in the publication


substrate_name: cortisone

proof_lines: 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli

proof_lines: 37°C, pH 7.4, recombinant 11beta-HSD1 measured in intact COS cells transfected with 11beta-HSD1

proof_lines: 37°C, pH 7.4, reduction of cortisone by lysates of HEK-293 cells transfected with 11beta-HSD1

substrate: cortisone


proof_lines: reduction of corticosterone, pH and temperature not specified in the publication

proof_lines: kidney homogenate, pH and temperature not specified in the publication


assay_conditions_summary: cofactor NAD+


proof_lines: 37°C, pH 7.4, V149R mutant 11beta-HSD1, V149E mutant 11beta-HSD1, and N-terminal 15 amino acid truncated V149R and V149E mutant 11beta-HSD1


substrate_name: cortisol

substrate: cortisol


proof_lines: 37°C, pH 7.4, oxidation of cortisol by lysates of HEK-293 cells transfected with 11beta-HSD1

proof_lines: 37°C, pH 7.4, oxidation of cortisol in intact HEK-293 cells transfected with 11beta-HSD1


substrate_name: metapyrone

proof_lines: microsomal fraction from transformed Pichia pastoris strain with human enzyme

substrate: metapyrone


substrate_name: oracin

substrate: oracin


proof_lines: isoform 11beta-HSD2, at pH 7.4, temperature not specified in the publication


proof_lines: recombinant isoform 11beta-HSD1, at pH 7.4 and 37°C

proof_lines: recombinant isoform 11beta-HSD2, at pH 7.4 and 37°C


enzyme_display_name: sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming)

organism: Bombyx mori

substrate_name: sepiapterin

assay_conditions_summary: pH 6.4; 310.15 K; buffer 100 mM potassium phosphate

proof_lines: recombinant enzyme, in 100 mM potassium phosphate buffer (pH 6.4), at 37°C

substrate: sepiapterin


organism: Musca domestica

enzyme: sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming)


enzyme_display_name: 2-dehydropantoate 2-reductase

organism: Corynebacterium glutamicum

enzyme: 2-dehydropantoate 2-reductase


enzyme_display_name: long-chain-alcohol dehydrogenase

organism: Geobacillus thermodenitrificans

assay_conditions_summary: pH 8; 333.15 K; cofactor NAD+

enzyme: long-chain-alcohol dehydrogenase


assay_conditions_summary: pH 8; 333.15 K; cofactor NADP+


substrate_name: NADPH

assay_conditions_summary: pH 8; 333.15 K; cofactor NADPH

substrate: NADPH


substrate_name: octanal

smiles: CCCCCCCC=O

substrate: octanal


smiles: CCCCCCCCO


substrate_name: prostaglandin E1

substrate: prostaglandin E1


enzyme_display_name: IMP dehydrogenase

organism: Klebsiella aerogenes

enzyme: IMP dehydrogenase


substrate_name: inosine 5'-phosphate

proof_lines: 6-thioinosine 5'-phosphate, constant in pH-range 6.5-8.2, sharp decrease at higher pH

substrate: inosine 5'-phosphate


substrate_name: NAD+

substrate: NAD+


organism: Legionella pneumophila subsp. pneumophila

substrate_name: IMP

proof_lines: pH 10.0, 30°C, recombinant enzyme, with MgATP2-

proof_lines: pH 10.0, 30°C, recombinant enzyme, without MgATP2-

substrate: IMP


assay_conditions_summary: pH 10; 303.15 K; cofactor NAD+


organism: Pneumocystis carinii


organism: Tritrichomonas foetus


assay_conditions_summary: pH 8; 298.15 K; cofactor NAD+


enzyme_display_name: (-)-menthol dehydrogenase

organism: Mentha arvensis

substrate_name: (-)-menthol

enzyme: (-)-menthol dehydrogenase

substrate: (-)-menthol


substrate_name: (-)-menthone

substrate: (-)-menthone


enzyme_display_name: gluconate 2-dehydrogenase

organism: Acetobacter pasteurianus

proof_lines:
+ 5-keto-D-gluconate
+ 2-keto-D-galactonate
+ 2-keto-L-gulonate

enzyme: gluconate 2-dehydrogenase


enzyme_display_name: farnesol dehydrogenase

organism: Aedes aegypti

smiles: CC(=CCCC(=CCCC(=CCO)C)C)C

enzyme: farnesol dehydrogenase


substrate_name: (Z)-nerol

smiles: CC(=CCCC(=CCO)C)C

substrate: (Z)-nerol


substrate_name: citronellol

smiles: CC(CCC=C(C)C)CCO

substrate: citronellol


smiles: CCCCCCCCC(C)O


substrate_name: Octanol

substrate: Octanol


enzyme_display_name: dihydroflavanol 4-reductase

organism: Delphinium grandiflorum

substrate_name: dihydromyricetin

enzyme: dihydroflavanol 4-reductase

substrate: dihydromyricetin


substrate_name: dihydroquercetin

substrate: dihydroquercetin


substrate_name: dihydrokaempferol

substrate: dihydrokaempferol


enzyme_display_name: sn-glycerol-1-phosphate dehydrogenase

organism: Methanothermobacter thermautotrophicus

enzyme: sn-glycerol-1-phosphate dehydrogenase


substrate_name: NADH

assay_conditions_summary: cofactor NADH

substrate: NADH


substrate_name: NADP+

substrate: NADP+


substrate_name: dihydroxyacetone phosphate

smiles: C(C(=O)COP(=O)(O)O)O

assay_conditions_summary: cofactor NADPH

proof_lines:
in the presence of NADPH
in the presence of NADH

substrate: dihydroxyacetone phosphate


enzyme_display_name: 3beta-hydroxysteroid 3-dehydrogenase

organism: Oryctolagus cuniculus

enzyme: 3beta-hydroxysteroid 3-dehydrogenase


substrate_name: methylglyoxal

smiles: CC(=O)C=O

substrate: methylglyoxal


proof_lines: pH 7.4, 25°C, wild-type enzyme and mutant F303Q


substrate_name: NADPH

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADPH

substrate: NADPH


enzyme_display_name: GDP-L-fucose synthase

organism: Mortierella alpina

assay_conditions_summary: pH 8; 310.15 K; cofactor NADPH

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C

enzyme: GDP-L-fucose synthase


assay_conditions_summary: pH 8; 310.15 K; cofactor NADH


enzyme_display_name: diacetyl reductase [(S)-acetoin forming]

organism: Bacillus licheniformis

smiles: CC(C(=O)C)O


smiles: CC(C(C)O)O

enzyme: diacetyl reductase [(S)-acetoin forming]


substrate_name: diacetyl

smiles: CC(=O)C(=O)C

substrate: diacetyl


assay_conditions_summary: pH 6; 303.15 K; cofactor NADH


assay_conditions_summary: pH 7; 298.15 K; cofactor NADH

proof_lines:
cosubstrate acetoin, pH 7.0, 25°C
cosubstrate diacetyl, pH 7.0, 25°C


smiles: CCC(=O)C(=O)C


substrate_name: Methyl pyruvate

smiles: CC(=O)C(=O)OC

substrate: Methyl pyruvate


substrate_name: ethyl pyruvate

smiles: CCOC(=O)C(=O)C

substrate: ethyl pyruvate


substrate_name: methyl glyoxal

substrate: methyl glyoxal


enzyme_display_name: D-xylose reductase [NAD(P)H]

organism: Saccharomyces cerevisiae

substrate_name: D-xylose

assay_conditions_summary: pH 7; 298.15 K; cofactor NADPH

proof_lines:
pH 7.0, 25°C, mutant K274M, with NADPH
pH 7.0, 25°C, mutant K274M, with NADH

enzyme: D-xylose reductase [NAD(P)H]

substrate: D-xylose


proof_lines: pH 7.0, 25°C, wild-type enzyme, with NADH

proof_lines: pH 7.0, 25°C, wild-type enzyme, with NADPH


proof_lines: pH 7.0, 25°C, mutant K274M/N276D, with NADH

proof_lines: pH 7.0, 25°C, mutant K274M/N276D, with NADPH


proof_lines:
pH 7.0, 25°C, mutant N276D, with NADH
pH 7.0, 25°C, mutant N276D, with NADPH


enzyme_display_name: nicotine blue oxidoreductase

organism: Paenarthrobacter nicotinovorans

proof_lines: pH 7.1, temperature not specified in the publication

enzyme: nicotine blue oxidoreductase


substrate_name: oxidized 2,6-dichlorophenol-indophenol

substrate: oxidized 2,6-dichlorophenol-indophenol


assay_conditions_summary: pH 7.1; cofactor NADH


enzyme_display_name: 2-dehydropantolactone reductase

organism: Candida dubliniensis

enzyme: 2-dehydropantolactone reductase


proof_lines: pH 7.0, 30°C, recombinant wild-type enzyme


proof_lines: pH 7.0, 30°C, recombinant immobilized enzyme


enzyme_display_name: (S)-malate dehydrogenase (NAD(+), oxaloacetate-forming)

organism: Corynebacterium glutamicum

substrate_name: oxaloacetate

substrate: oxaloacetate


assay_conditions_summary: pH 8; 296.15 K; cofactor NADH

enzyme: (S)-malate dehydrogenase (NAD(+), oxaloacetate-forming)


assay_conditions_summary: pH 6.5; 296.15 K; cofactor NADPH


substrate_name: NAD+

assay_conditions_summary: pH 10.5; 296.15 K; cofactor NAD+

substrate: NAD+


substrate_name: L-malate

substrate: L-malate


organism: Fasciola gigantica

assay_conditions_summary: pH 8; 298.15 K; cofactor NADH


substrate_name: (S)-malate

substrate: (S)-malate


organism: Gallus sp.


organism: Rhodobacter capsulatus


assay_conditions_summary: cofactor NAD+


substrate_name: Ketomalonate

substrate: Ketomalonate


organism: Proteus vulgaris


enzyme_display_name: ketol-acid reductoisomerase [NAD(P)(+)]

organism: Metallosphaera sedula

assay_conditions_summary: pH 7; cofactor NADH

proof_lines: pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate

enzyme: ketol-acid reductoisomerase [NAD(P)(+)]


assay_conditions_summary: pH 7; cofactor NADPH


enzyme_display_name: malate dehydrogenase (decarboxylating)

organism: Solanum tuberosum

assay_conditions_summary: cofactor NADP+

proof_lines:
with NAD+ and Mn2+
with NADP+ and Mn2+
with NADP+ and Mg2+

enzyme: malate dehydrogenase (decarboxylating)


proof_lines:
activation by Mn2+
with NADP+ as coenzyme


proof_lines:
activation by Mn2+
activation by Mg2+


enzyme_display_name: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+))

organism: Columba sp.


assay_conditions_summary: pH 4.5; 298.15 K; cofactor NADP+

enzyme: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+))


enzyme_display_name: 2-alkyl-3-oxoalkanoate reductase

organism: Stenotrophomonas maltophilia

substrate_name: (2R/2S)-2-hexyl-3-oxodecanoic acid

smiles: CCCCCCCC(=O)C(CCCCCC)C(=O)O

enzyme: 2-alkyl-3-oxoalkanoate reductase

substrate: (2R/2S)-2-hexyl-3-oxodecanoic acid


substrate_name: syn-2-hexyl-3-hydroxydecanoic acid

substrate: syn-2-hexyl-3-hydroxydecanoic acid


substrate_name: (2R,3S)-2-hexyl-3-hydroxydecanoic acid

smiles: CCCCCCCC(C(CCCCCC)C(=O)O)O

substrate: (2R,3S)-2-hexyl-3-hydroxydecanoic acid


substrate_name: NADP+

assay_conditions_summary: pH 8; 298.15 K; cofactor NADP+

substrate: NADP+


assay_conditions_summary: pH 8; 298.15 K; cofactor NADPH


enzyme_display_name: isocitrate dehydrogenase (NADP(+))

organism: Methanococcoides methylutens

assay_conditions_summary: pH 8.2; 318.15 K; cofactor NAD+

proof_lines: mutant enzyme H590L/R601D, in the presence of Mn2+, at pH 8.2 and 45°C

enzyme: isocitrate dehydrogenase (NADP(+))


assay_conditions_summary: pH 8.2; 318.15 K; cofactor NADP+


proof_lines: wild type enzyme, in the presence of Mn2+, at pH 8.2 and 45°C


proof_lines: mutant enzyme H590L/R601D/R650S, in the presence of Mn2+, at pH 8.2 and 45°C


organism: Pseudomonas psychrophila

substrate_name: isocitrate

substrate: isocitrate


enzyme_display_name: phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating)


enzyme: phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating)


organism: Daucus carota


organism: Gallus gallus


organism: Geobacillus stearothermophilus

proof_lines:
free enzyme, at pH 7.5 and 25°C
60°C or 65°C
30°C


assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADP+


organism: Lathyrus oleraceus


organism: Vigna radiata


enzyme_display_name: glucose 1-dehydrogenase [NAD(P)(+)]

organism: Rattus norvegicus

substrate_name: D-glucose 6-phosphate

smiles: C(C(C(C(C(C=O)O)O)O)O)OP(=O)(O)O

proof_lines: pH not specified in the publication, temperature not specified in the publication

enzyme: glucose 1-dehydrogenase [NAD(P)(+)]

substrate: D-glucose 6-phosphate


organism: Cryptococcus neoformans


organism: Cyberlindnera jadinii


organism: Leishmania mexicana


organism: Homo sapiens

substrate_name: androstenedione

substrate: androstenedione


substrate_name: estrone

substrate: estrone


substrate_name: testosterone

proof_lines: enzyme activity in cell-free homogenate, approximate value

substrate: testosterone


substrate_name: Unknown

proof_lines: pH 7.0, temperature not specified in the publication

proof_lines: isoform 17beta-hydroxysteroid dehydrogenase type 3, pH 7.4, 37°C

proof_lines: isoform 17beta-hydroxysteroid dehydrogenase type 3 , pH 7.4, 37°C

substrate: Unknown


enzyme_display_name: hydroxypyruvate reductase

organism: Sinorhizobium meliloti

substrate_name: NADPH

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

enzyme: hydroxypyruvate reductase

substrate: NADPH


substrate_name: phenylpyruvate

substrate: phenylpyruvate


proof_lines: K0.5 value, Hill coefficient 1.74, pH 7.5, 25°C


substrate_name: pyruvate

substrate: pyruvate


substrate_name: glyoxylate

proof_lines: K0.5 value, Hill coefficient 1.83, pH 7.5, 25°C

proof_lines: K0.5 value, Hill coefficient 1.48, pH 7.5, 25°C

substrate: glyoxylate


enzyme_display_name: 3-isopropylmalate dehydrogenase

organism: Salmonella enterica subsp. enterica serovar Typhimurium

assay_conditions_summary: pH 9

enzyme: 3-isopropylmalate dehydrogenase


organism: Shewanella benthica

substrate_name: NAD+

assay_conditions_summary: pH 7.6; 298.15 K; cofactor NAD+

substrate: NAD+


proof_lines: pressure 250 MPa, 20°C, pH not specified in the publication

proof_lines: pressure 200 MPa, 20°C, pH not specified in the publication

proof_lines: pressure 100 MPa, 20°C, pH not specified in the publication


enzyme_display_name: ketol-acid reductoisomerase (NADP(+))

proof_lines: 30°C, with 2-acetolactate or 2-aceto-2-hydroxybutyrate as substrate

enzyme: ketol-acid reductoisomerase (NADP(+))


smiles: CCC(C(=O)C)(C(=O)O)O


assay_conditions_summary: 303.15 K; cofactor NADPH

proof_lines: 30°C with 2-acetolactate or 2-aceto-2-hydroxybutyrate as substrate


organism: Streptomyces lividans


substrate_name: Hydroxypyruvate

substrate: Hydroxypyruvate


enzyme_display_name: aryl-alcohol dehydrogenase

organism: Pseudomonas putida

substrate_name: benzaldehyde

enzyme: aryl-alcohol dehydrogenase

substrate: benzaldehyde


proof_lines:
chromosome-encoded enzyme
plasmid-encoded enzyme


substrate_name: NADH

assay_conditions_summary: cofactor NADH

substrate: NADH


substrate_name: 3-methylbenzyl alcohol

proof_lines:
plasmid-encoded enzyme
chromosome-encoded enzyme

substrate: 3-methylbenzyl alcohol


substrate_name: 4-methylbenzyl alcohol

substrate: 4-methylbenzyl alcohol


substrate_name: 2-Methylbenzyl alcohol

substrate: 2-Methylbenzyl alcohol


substrate_name: benzyl alcohol

substrate: benzyl alcohol


smiles: CC(=CCO)C


enzyme_display_name: phosphoglycerate dehydrogenase

organism: Entamoeba histolytica

substrate_name: phosphohydroxypyruvate

enzyme: phosphoglycerate dehydrogenase

substrate: phosphohydroxypyruvate


assay_conditions_summary: pH 6.5; cofactor NADH


assay_conditions_summary: pH 9; cofactor NAD+


assay_conditions_summary: pH 6.5; cofactor NADPH


assay_conditions_summary: 298.15 K; cofactor NADH


assay_conditions_summary: 298.15 K; cofactor NAD+


assay_conditions_summary: cofactor NAD+


organism: Mycobacterium marinum

assay_conditions_summary: pH 7; buffer ranking_score=0.0. Quote: "in 200 mM KPO4

proof_lines: in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication

proof_lines: in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication


assay_conditions_summary: pH 7; buffer 50 mM MOPS


assay_conditions_summary: pH 7


assay_conditions_summary: pH 7; cofactor NADH


assay_conditions_summary: pH 7; cofactor NAD+


enzyme_display_name: L-lactate dehydrogenase (cytochrome)

organism: Rhizopus arrhizus

substrate_name: L-lactate

proof_lines: 2,6-dichlorophenolindopenol as electron acceptor

enzyme: L-lactate dehydrogenase (cytochrome)

substrate: L-lactate


proof_lines:
10 mM
1 mM


enzyme_display_name: methanol dehydrogenase (cytochrome c)

organism: Methylosinus sp.

substrate_name: sorbic alcohol

smiles: CC=CC=CCO

proof_lines: pH 9.0, 57°C, acceptor: 2,6-dichlorophenolindophenol

enzyme: methanol dehydrogenase (cytochrome c)

substrate: sorbic alcohol


substrate_name: methanol

smiles: CO

substrate: methanol


substrate_name: Heptanol

smiles: CCCCCCCO

substrate: Heptanol


substrate_name: Octanol

smiles: CCCCCCCCO

substrate: Octanol


substrate_name: Hexanol

smiles: CCCCCCO

substrate: Hexanol


substrate_name: Pentanol

smiles: CCCCCO

proof_lines: pH 9.0, 57°C, acceptor 2,6-dichlorophenolindophenol

substrate: Pentanol


substrate_name: ethanol

smiles: CCO

substrate: ethanol


substrate_name: Propanol

smiles: CCCO

substrate: Propanol


substrate_name: butanol

smiles: CCCCO

substrate: butanol


enzyme_display_name: alcohol oxidase

organism: Pichia sp.

enzyme: alcohol oxidase


substrate_name: formaldehyde

smiles: C=O

substrate: formaldehyde


organism: [Candida] boidinii

smiles: C(C#CC=O)O


smiles: C(C#CCO)O


enzyme_display_name: (S)-2-hydroxy-acid oxidase

substrate_name: L-Mandelate

enzyme: (S)-2-hydroxy-acid oxidase

substrate: L-Mandelate


smiles: CCCCCCC(=O)C(=O)[O-]


substrate_name: glyoxylate

substrate: glyoxylate


substrate_name: flavin

proof_lines:
pH 7.0, 30°C, flavin reduction
pH 7.0, 25°C

substrate: flavin


smiles: O=O

proof_lines: pH 7.5, 30°C, recombinant enzyme, first order

proof_lines: pH 9.0, 30°C, recombinant enzyme, first order

proof_lines: pH 7.0, 30°C, recombinant enzyme, first order


enzyme_display_name: glycerol-3-phosphate oxidase

organism: Streptococcus sp.

substrate_name: sn-glycerol 3-phosphate

mutation_signature: recombinant deletion mutant

smiles: C(C(COP(=O)(O)O)O)O

proof_lines: 10 microM enzyme and 0.77 mM O2, substrate concentration ranges between 2.5 and 50 mM, pH 7.0, 5°C, recombinant deletion mutant

enzyme: glycerol-3-phosphate oxidase

substrate: sn-glycerol 3-phosphate


proof_lines: 10 microM enzyme and 0.77 mM O2, substrate concentration ranges between 2.5 and 50 mM, pH 7.0, 5°C, wild-type enzyme, nicked

proof_lines: 10 microM enzyme and 0.77 mM O2, substrate concentration ranges between 2.5 and 50 mM, pH 7.0, 5°C, wild-type enzyme, intact


proof_lines:
pH 7.0, 5°C, wild-type enzyme, intact
pH 7.0, 5°C, wild-type enzyme, nicked


proof_lines: pH 7.0, 5°C, recombinant deletion mutant


proof_lines:
intact enzyme form, at pH 7.0 and 25°C
nicked enzyme form, at pH 7.0 and 25°C


mutation_signature: deletion mutant GlpODELTA

proof_lines: deletion mutant GlpODELTA, at pH 7.0 and 25°C


enzyme_display_name: cholesterol oxidase

organism: Streptomyces hygroscopicus

substrate_name: epiandrosterone

assay_conditions_summary: pH 7.5; 298.15 K; buffer substrate trans-androsterone

proof_lines: H2O2 production assayed, 100 mM phosphate buffer

proof_lines: substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5

enzyme: cholesterol oxidase

substrate: epiandrosterone


mutation_signature: stopped-flow measurement / stopped-flow measurement

proof_lines: isomerization to 4-cholesten-3-one, rapid reaction, stopped-flow measurement, 500 mM phosphate buffer, pH 7.5, in the presence of 1% thesit and 1.25% 2-propanol

proof_lines: isomerization to 4-cholesten-3-one, rapid reaction, stopped-flow measurement, 50 mM phosphate buffer, pH 7.5, in the presence of 1% thesit and 1.25% 2-propanol


substrate_name: cholestanol

assay_conditions_summary: pH 7.5; 298.15 K; buffer substrate cholestanol

proof_lines: assay relies on detection of H2O2 formation

proof_lines: substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5

substrate: cholestanol


substrate_name: cholesterol

assay_conditions_summary: pH 7.5; 298.15 K; buffer product formation followed

proof_lines: substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5

proof_lines: substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5

proof_lines: assay relies on spectroscopic detection of 4-cholesten-3-one formation

substrate: cholesterol


substrate_name: trans-dehydroandrosterone

assay_conditions_summary: pH 7.5; 298.15 K; buffer substrate trans-dehydroandrosterone

proof_lines: substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5

substrate: trans-dehydroandrosterone


substrate_name: pregnenolone

assay_conditions_summary: pH 7.5; 298.15 K; buffer substrate pregnenolone

proof_lines: substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5

proof_lines: product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer

substrate: pregnenolone


enzyme_display_name: L-gulonolactone oxidase

organism: Gallus gallus

enzyme: L-gulonolactone oxidase


organism: Rattus norvegicus


substrate_name: creatol

assay_conditions_summary: pH 9

proof_lines:
at pH 9.0
at pH 7.4

substrate: creatol


enzyme_display_name: malate dehydrogenase (quinone)

organism: Pseudomonas taetrolens

substrate_name: maltose

enzyme: malate dehydrogenase (quinone)

substrate: maltose


substrate_name: (S)-malate

substrate: (S)-malate


enzyme_display_name: alcohol dehydrogenase (azurin)

organism: Comamonas testosteroni

substrate_name: benzyl alcohol

enzyme: alcohol dehydrogenase (azurin)

substrate: benzyl alcohol


proof_lines: soluble enzyme, pH 7.7, presence of CaCl2


proof_lines: enzyme electrode, pH 7.7, presence of CaCl2


substrate_name: octanal

smiles: CCCCCCCC=O

substrate: octanal


substrate_name: Butanal

smiles: CCCC=O

substrate: Butanal


smiles: C(CCCO)CCN


smiles: CC(CCO)O


substrate_name: Ethanal

smiles: CC=O

proof_lines:
pH 7.5, presence of CaCl2
pH 7.5, 25°C
pH 7.0, 20°C

substrate: Ethanal


substrate_name: ethanol

substrate: ethanol


substrate_name: (S)-solketal

substrate: (S)-solketal


substrate_name: (R)-solketal aldehyde

proof_lines:
pH 7.5, presence of CaCl2
pH 7.5, 25°C

substrate: (R)-solketal aldehyde


smiles: CC(CO)CBr


enzyme_display_name: D-sorbitol dehydrogenase (acceptor)

organism: Gluconobacter oxydans

substrate_name: D-sorbitol

smiles: C(C(C(C(C(CO)O)O)O)O)O

proof_lines: phenazine methosulfate as electron acceptor

enzyme: D-sorbitol dehydrogenase (acceptor)

substrate: D-sorbitol


substrate_name: phenazine methosulfate

substrate: phenazine methosulfate


substrate_name: NADP+

assay_conditions_summary: pH 10; 298.15 K; cofactor NADP+

substrate: NADP+


enzyme_display_name: ferredoxin hydrogenase

organism: Clostridium acetobutylicum

smiles: [HH]

proof_lines: using ferredoxin CAC0587 as electron carrier, at 35°C

proof_lines: using methyl viologen as electron carrier, at 35°C

proof_lines: using flavodoxin CAC0587 as electron carrier, at 35°C

enzyme: ferredoxin hydrogenase


organism: Pyrococcus furiosus

assay_conditions_summary: cofactor NADP+


substrate_name: NADH

assay_conditions_summary: cofactor NADH

substrate: NADH


substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

substrate: NADPH


substrate_name: NAD+

substrate: NAD+


substrate_name: sulfur

smiles: S

substrate: sulfur


substrate_name: methyl viologen

substrate: methyl viologen


substrate_name: reduced methyl viologen

substrate: reduced methyl viologen


organism: Methanothermobacter marburgensis

substrate_name: Unknown

proof_lines: pH 6.0, 40°C, under N2, substrate concentration of 0.003 mM

proof_lines: pH 6.0, 40°C, under N2, substrate concentration of 0.01 mM

substrate: Unknown


enzyme_display_name: catechol 2,3-dioxygenase

organism: Acinetobacter sp.

substrate_name: catechol

enzyme: catechol 2,3-dioxygenase

substrate: catechol


organism: Saccharolobus solfataricus


enzyme_display_name: arachidonate 5-lipoxygenase

organism: Mus musculus

substrate_name: arachidonate

smiles: CCCCCC=CCC=CCC=CCC=CCCCC(=O)[O-]

proof_lines: wild-type, pH and temperature not specified in the publication

enzyme: arachidonate 5-lipoxygenase

substrate: arachidonate


mutation_signature: Δ1

proof_lines: mutant DELTA1-114, pH and temperature not specified in the publication


enzyme_display_name: hydroxyquinol 1,2-dioxygenase

organism: Burkholderia cepacia

substrate_name: Hydroxyquinol

enzyme: hydroxyquinol 1,2-dioxygenase

substrate: Hydroxyquinol


enzyme_display_name: chlorite O2-lyase

organism: Magnetospirillum sp.

substrate_name: Chlorite

smiles: [O-]Cl=O

enzyme: chlorite O2-lyase

substrate: Chlorite


enzyme_display_name: homogentisate 1,2-dioxygenase

substrate_name: homogentisate

enzyme: homogentisate 1,2-dioxygenase

substrate: homogentisate


enzyme_display_name: lactate 2-monooxygenase

organism: Mycolicibacterium smegmatis

substrate_name: (S)-lactate

enzyme: lactate 2-monooxygenase

substrate: (S)-lactate


proof_lines:
calculated constant, wild-type
observed constant, wild-type
wild-type


enzyme_display_name: codeine 3-O-demethylase

organism: Papaver somniferum

substrate_name: codeine

enzyme: codeine 3-O-demethylase

substrate: codeine


substrate_name: thebaine

substrate: thebaine


enzyme_display_name: naphthalene 1,2-dioxygenase

organism: Pseudomonas sp.

substrate_name: indole

enzyme: naphthalene 1,2-dioxygenase

substrate: indole


enzyme_display_name: phthalate 4,5-dioxygenase

substrate_name: Phthalate

proof_lines: wild type enzyme with its mononuclear center containing Fe(II)

proof_lines: wild type enzyme with its mononuclear center lacking Fe(II)

enzyme: phthalate 4,5-dioxygenase

substrate: Phthalate


proof_lines: mutant enzyme D178A with its mononuclear center lacking Fe(II)

proof_lines: mutant enzyme D178A with its mononuclear center containing Fe(II)


proof_lines: mutant enzyme D178N with its mononuclear center lacking Fe(II)

proof_lines: mutant enzyme D178N with its mononuclear center containing Fe(II)


organism: Pseudomonas putida

substrate_name: NADH

assay_conditions_summary: pH 8; 298.15 K; cofactor NADH

proof_lines: pH 8.0, 25°C, recombinant His-tagged enzyme

substrate: NADH


enzyme_display_name: toluene 4-monooxygenase

organism: Ectopseudomonas mendocina

substrate_name: Toluene

enzyme: toluene 4-monooxygenase

substrate: Toluene


substrate_name: methyl 4-tolyl sulfide

proof_lines: whole-cell assay, 25°C, pH not specified in the publication

substrate: methyl 4-tolyl sulfide


substrate_name: methyl phenyl sulfide

substrate: methyl phenyl sulfide


substrate_name: norcarane

substrate: norcarane


enzyme_display_name: nitrilotriacetate monooxygenase

organism: Aminobacter aminovorans

substrate_name: FMN

assay_conditions_summary: pH 8.5; 298.15 K; buffer 40 mM TAPS; cofactor FMN

enzyme: nitrilotriacetate monooxygenase

substrate: FMN


substrate_name: nitrilotriacetate

assay_conditions_summary: pH 7.6; 297.15 K; buffer equimolar Ni2+ (0.04-0.2 mM)" and "in 20 mM HEPES; cofactor FMN

proof_lines: with equimolar Ni2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C

proof_lines: with equimolar Co2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C

proof_lines: with equimolar Mg2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C

substrate: nitrilotriacetate


enzyme_display_name: sterol 14alpha-demethylase

organism: Trypanosoma cruzi

substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication

enzyme: sterol 14alpha-demethylase

substrate: Unknown


enzyme_display_name: ent-kaurene monooxygenase

organism: Cucurbita maxima

enzyme: ent-kaurene monooxygenase


enzyme_display_name: 4-hydroxyphenylacetate 3-monooxygenase

organism: Acinetobacter baumannii

assay_conditions_summary: pH 7.5; cofactor NADH

proof_lines: native C1-C2 system or complete enzyme, respectively, pH 7.5, with FMN as cosubstrate

enzyme: 4-hydroxyphenylacetate 3-monooxygenase


assay_conditions_summary: pH 7.5; cofactor FAD

proof_lines: native C1-C2 system or complete enzyme, respectively, pH 7.5

proof_lines: recombinant C1-C2 system or complete enzyme, respectively, pH 7.5


enzyme_display_name: sterol 22-desaturase

organism: Physcomitrium patens

substrate_name: beta-sitosterol

proof_lines:
isoform CAP710A14, at pH 7.3 and 30°C
isoform CAP710A13, at pH 7.3 and 30°C

enzyme: sterol 22-desaturase

substrate: beta-sitosterol


organism: Solanum lycopersicum


enzyme_display_name: noroxomaritidine synthase

organism: Narcissus aff. pseudonarcissus MK-2014

substrate_name: (S)-Coclaurine

enzyme: noroxomaritidine synthase

substrate: (S)-Coclaurine


enzyme_display_name: superoxide dismutase

organism: Avicennia marina

substrate_name: superoxide

enzyme: superoxide dismutase

substrate: superoxide


enzyme_display_name: cuproxidase

organism: Salmonella enterica subsp. enterica serovar Typhimurium

assay_conditions_summary: pH 5

proof_lines: pH 5, temperature not specified in the publication

enzyme: cuproxidase


enzyme_display_name: xanthine oxidase

organism: Rattus norvegicus

substrate_name: xanthine

enzyme: xanthine oxidase

substrate: xanthine


proof_lines: xanthine oxidoreductase mutant W335A/F336L, pH 7.8, 25°C

proof_lines: xanthine oxidoreductase mutant W335A/F336L treated with dithiothreitol, pH 7.8, 25°C


proof_lines: untreated C-terminally truncated enzyme mutant, pH 7.8, 25°C


proof_lines: DTT-treated C-terminally truncated enzyme mutant, pH 7.8, 25°C


organism: Lactobacillus leichmannii

substrate_name: adenosylcobalamin

proof_lines:
presence of 1.0 mM CTP and 1 mM dATP
presence of 0.1 mM CTP and 1 mM dATP
presence of 2 mM CTP without activator

substrate: adenosylcobalamin


substrate_name: CTP

assay_conditions_summary: cofactor ATP

proof_lines:
presence of 1 mM dATP
without ATP

substrate: CTP


substrate_name: ATP

substrate: ATP


substrate_name: UTP

substrate: UTP


substrate_name: dGTP

substrate: dGTP


organism: Gallus gallus


enzyme_display_name: caffeine dehydrogenase

substrate_name: Caffeine

enzyme: caffeine dehydrogenase

substrate: Caffeine


organism: Citrobacter sp. S-77

substrate_name: acetyl-CoA

assay_conditions_summary: pH 7; 303.15 K; cofactor CoA

substrate: acetyl-CoA


substrate_name: acetaldehyde

substrate: acetaldehyde


organism: Moorella sp. HUC22-1

assay_conditions_summary: pH 8; 333.15 K; cofactor CoA


enzyme_display_name: aspartate-semialdehyde dehydrogenase

organism: Amycolatopsis mediterranei U32

substrate_name: NADP+

enzyme: aspartate-semialdehyde dehydrogenase

substrate: NADP+


substrate_name: L-aspartate 4-semialdehyde

substrate: L-aspartate 4-semialdehyde


organism: Candida albicans


organism: Bacillus cereus

substrate_name: NAD+

assay_conditions_summary: cofactor NAD+

substrate: NAD+


substrate_name: D-glyceraldehyde 3-phosphate

smiles: C(C(C=O)O)OP(=O)(O)O

substrate: D-glyceraldehyde 3-phosphate


substrate_name: arsenate

substrate: arsenate


organism: Dissostichus mawsoni


substrate_name: thio-NAD+

substrate: thio-NAD+


organism: Euglena gracilis


substrate_name: NADH

substrate: NADH


substrate_name: 3-phospho-D-glyceroyl phosphate

substrate: 3-phospho-D-glyceroyl phosphate


organism: Fasciola gigantica

assay_conditions_summary: pH 7.4; 310.15 K; cofactor NAD+


enzyme_display_name: benzaldehyde dehydrogenase (NAD(+))

organism: Pseudomonas putida

substrate_name: benzaldehyde

assay_conditions_summary: pH 9.6; buffer glycine-KOH

proof_lines: apparent Kcat-value, pH 9.6, temperature not specified in the publication

proof_lines: in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication

proof_lines: apparent Km-value, pH 9.6, temperature not specified in the publication

enzyme: benzaldehyde dehydrogenase (NAD(+))

substrate: benzaldehyde


assay_conditions_summary: pH 9.6; cofactor NAD+

proof_lines: apparent Km-value at substrate saturation, pH 9.6, temperature not specified in the publication


assay_conditions_summary: pH 8.5; 303.15 K; cofactor NAD+

proof_lines: pH 8.5, 30°C, recombinant mutant E337Q, with NAD+


proof_lines: pH 8.5, 30°C, recombinant mutant E337L, with NAD+


proof_lines: pH 8.5, 30°C, recombinant mutant E215D, with NAD+


proof_lines: pH 8.5, 30°C, recombinant mutant E337D, with NAD+


proof_lines: pH 8.5, 30°C, recombinant wild-type enzyme, with NAD+


enzyme_display_name: aldehyde dehydrogenase (NAD(+))

organism: Acanthopagrus schlegelii

substrate_name: propanal

smiles: CCC=O

enzyme: aldehyde dehydrogenase (NAD(+))

substrate: propanal


substrate_name: octanal

substrate: octanal


assay_conditions_summary: pH 9.5; cofactor NAD+


enzyme_display_name: phenylacetaldehyde dehydrogenase

assay_conditions_summary: pH 8.5; 298.15 K; cofactor NAD+

enzyme: phenylacetaldehyde dehydrogenase


enzyme_display_name: formaldehyde dehydrogenase

organism: Homo sapiens

substrate_name: S-hydroxymethylglutathione

proof_lines: oxidation of S-hydroxymethylglutathione, formaldehyde dehydrogenase

enzyme: formaldehyde dehydrogenase

substrate: S-hydroxymethylglutathione


assay_conditions_summary: cofactor NADH

proof_lines: oxidation of NADH, formaldehyde dehydrogenase


proof_lines: reduction of NAD+, formaldehyde dehydrogenase


substrate_name: S-nitrosoglutathione

proof_lines: reduction of S-nitrosoglutathione, formaldehyde dehydrogenase

substrate: S-nitrosoglutathione


proof_lines: oxidation of S-hydroxymethylglutathione, R368L mutant


proof_lines: reduction of S-nitrosoglutathione, R368L mutant


proof_lines: oxidation of S-hydroxymethylglutathione, E67L mutant


substrate_name: Unknown

substrate: Unknown


proof_lines: pH 7.5, temperature not specified in the publication


smiles: C(CC=O)CN=C(N)N


smiles: CCCC=CC=O


assay_conditions_summary: pH 7.5; cofactor NAD+


substrate_name: Valeraldehyde

smiles: CCCCC=O

substrate: Valeraldehyde


substrate_name: hexanal

smiles: CCCCCC=O

substrate: hexanal


smiles: C(CN)C=O


smiles: C(CC=O)CN


substrate_name: Butyraldehyde

smiles: CCCC=O

substrate: Butyraldehyde


smiles: CC(C)CC(C)(C=O)N


smiles: CCCCCCCC=O


substrate_name: Heptanal

smiles: CCCCCCC=O

substrate: Heptanal


substrate_name: hexadecanal

smiles: CCCCCCCCCCCCCCCC=O

substrate: hexadecanal


substrate_name: pentanal

substrate: pentanal


substrate_name: propionaldehyde

substrate: propionaldehyde


substrate_name: NAD+

substrate: NAD+


substrate_name: acetaldehyde

smiles: CC=O

substrate: acetaldehyde


substrate_name: Betaine aldehyde

substrate: Betaine aldehyde


substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

substrate: NADP+


enzyme_display_name: salicylaldehyde dehydrogenase

organism: Pseudomonas sp.

assay_conditions_summary: pH 8

proof_lines: pH 8.0, temperature not specified in the publication

enzyme: salicylaldehyde dehydrogenase


substrate_name: benzaldehyde

substrate: benzaldehyde


substrate_name: Salicylaldehyde

substrate: Salicylaldehyde


assay_conditions_summary: pH 8; cofactor NAD+


enzyme_display_name: vanillin dehydrogenase

organism: Burkholderia cepacia

substrate_name: Vanillin

assay_conditions_summary: pH 7

proof_lines: pH 7, temperature not specified in the publication

enzyme: vanillin dehydrogenase

substrate: Vanillin


enzyme_display_name: betaine-aldehyde dehydrogenase

organism: Leymus chinensis

enzyme: betaine-aldehyde dehydrogenase


assay_conditions_summary: pH 8; 303.15 K; cofactor NAD+


smiles: C[N+](C)(C)CCCC=O


assay_conditions_summary: pH 8; 303.15 K; cofactor NADP+


organism: Nostoc punctiforme

substrate_name: stearoyl-[acyl-carrier protein]

proof_lines: presence of potassium ions, pH 7.6, 37°C

substrate: stearoyl-[acyl-carrier protein]


organism: Synechococcus elongatus

substrate_name: oleoyl-[acyl carrier protein]

proof_lines: pH and temperature not specified in the publication

substrate: oleoyl-[acyl carrier protein]


organism: Streptococcus mutans


proof_lines:
pH 8.5, 25°C, mutant enzyme R459I
pH 8.4, 25°C, mutant enzyme R459I


proof_lines:
pH 8.5, 25°C, wild-type enzyme
pH 8.4, 25°C, wild-type enzyme


substrate_name: Unknown

substrate: Unknown


enzyme_display_name: aldehyde oxidase

substrate_name: clonazepam

enzyme: aldehyde oxidase

substrate: clonazepam


substrate_name: nimetazepam

substrate: nimetazepam


substrate_name: nilutamide

substrate: nilutamide


substrate_name: flunitrazepam

substrate: flunitrazepam


substrate_name: flutamide

substrate: flutamide


substrate_name: nimesulide

substrate: nimesulide


proof_lines: metabolite 2-oxo-5-aminoquinoline, pH 7.4, 37°C

proof_lines: metabolite 2-oxo-5-nitroquinoline, pH 7.4, 37°C

proof_lines: metabolite 5-aminoquinoline, presence of N-(2-(dimethylamino)ethyl)acridine-4-carboxamide, pH 7.4, 37°C


substrate_name: methotrexate

substrate: methotrexate


proof_lines: liver cytosol, pH not specified in the publication, temperature not specified in the publication

proof_lines: Ki-value for free enzyme with DACA as substrate, assay at 100 microM concentration, pH 7.4 and 37°C


substrate_name: zebularine

proof_lines:
male, pH 7.5, 37°C
female, pH 7.5, 37°C

substrate: zebularine


substrate_name: zoniporide

assay_conditions_summary: 310.15 K; buffer 25 mM phosphate

proof_lines: in 25 mM phosphate buffer at 37°C, pH not specified in the publication

substrate: zoniporide


organism: Corynebacterium glutamicum

substrate_name: pyruvate

substrate: pyruvate


organism: Oryctolagus cuniculus


smiles: CC(C)CC(=O)C(=O)O


enzyme_display_name: aerobic carbon monoxide dehydrogenase

organism: Afipia carboxidovorans

substrate_name: CO

smiles: [Co]

proof_lines: pH 7.2, 25°C, reductive half-reaction of enzyme CODH with CO

enzyme: aerobic carbon monoxide dehydrogenase

substrate: CO


enzyme_display_name: pyruvate synthase

organism: Desulfovibrio piger

substrate_name: CoA

assay_conditions_summary: pH 8.5; 308.15 K; cofactor CoA

enzyme: pyruvate synthase

substrate: CoA


organism: Thermotoga maritima

assay_conditions_summary: pH 8.4; 353.15 K; cofactor CoA


organism: Methanothermobacter thermautotrophicus

assay_conditions_summary: pH 8; 338.15 K; cofactor CoA


smiles: CC(C)C(=O)C(=O)O


organism: Thermococcus profundus

assay_conditions_summary: pH 9; 353.15 K; cofactor CoA


substrate_name: phenylpyruvate

assay_conditions_summary: pH 9; 353.15 K; buffer 50 mM TAPS

proof_lines:
in 50 mM TAPS buffer (pH 9.0), at 80°C
pH 9.0, 80°C

substrate: phenylpyruvate


substrate_name: oxidized ferredoxin

substrate: oxidized ferredoxin


organism: Sulfolobus acidocaldarius

substrate_name: propionaldehyde

smiles: CCC=O

substrate: propionaldehyde


substrate_name: DL-glyceraldehyde

smiles: C(C(C=O)O)O

substrate: DL-glyceraldehyde


organism: Vitis vinifera

substrate_name: cyanidin

substrate: cyanidin


substrate_name: NADPH

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADP+

substrate: NADPH


enzyme_display_name: prephenate dehydrogenase

organism: Aquifex aeolicus

substrate_name: NAD+

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NAD+

proof_lines:
at 30°C, wild-type
at 55°C, wild-type
wild-type, pH 7.5, 55°C

enzyme: prephenate dehydrogenase

substrate: NAD+


substrate_name: prephenate

proof_lines:
at 30°C, wild-type
at 80°C, wild-type
at 55°C, wild-type

substrate: prephenate


substrate_name: L-arogenate

substrate: L-arogenate


assay_conditions_summary: 303.15 K; cofactor NAD+

proof_lines:
at 30°C, delta19PD
at 55°C, delta19PD
at 80°C, delta19PD


substrate_name: Unknown

substrate: Unknown


organism: Klebsiella aerogenes

assay_conditions_summary: cofactor NAD+


assay_conditions_summary: pH 6.8; 310.15 K; cofactor NAD+


organism: Macaca fascicularis

substrate_name: Camphorquinone

assay_conditions_summary: cofactor NADPH

proof_lines: reduction reaction of mutant K97M, 0.1 mM NADPH

proof_lines: reduction reaction of mutant K97M, using 0.1 mM NADPH as coenzyme

substrate: Camphorquinone


proof_lines: reduction reaction of mutant K79R, 0.1 mM NADPH

proof_lines: reduction reaction of mutant K79R, using 0.1 mM NADPH as coenzyme


proof_lines: reduction oxidation reaction of mutant A36D, 0.1 mM NADPH

proof_lines: reduction reaction of mutant A36D, using 0.1 mM NADPH as coenzyme


proof_lines: reduction oxidation reaction of mutante R37D, 0.1 mM NADPH

proof_lines: reduction reaction of mutant R37D, using 0.1 mM NADPH as coenzyme


proof_lines: reduction reaction of mutant H79Q, 0.1 mM NADPH

proof_lines: reduction reaction of mutant H79Q, using 0.1 mM NADPH as coenzyme


proof_lines: oxidation reaction of mutant R41D, 0.1 mM NADPH

proof_lines: reduction reaction of mutant R41D, using 0.1 mM NADPH as coenzyme


proof_lines: reduction reaction of mutant R37A, 0.1 mM NADPH

proof_lines: reduction reaction of mutant R37A, using 0.1 mM NADPH as coenzyme


proof_lines: reduction reaction of mutant H76Q, 0.1 mM NADPH

proof_lines: reduction reaction of mutant H76Q, using 0.1 mM NADPH as coenzyme


proof_lines: reduction reaction of wild type enzyme, 0.1 mM NADPH

proof_lines: reduction reaction of wild type enzyme, using 0.1 mM NADPH as coenzyme


proof_lines: reduction reaction of mutant W254Y, using 0.1 mM NADPH as coenzyme


proof_lines: reduction reaction of mutant F154A, using 0.1 mM NADPH as coenzyme


proof_lines: reduction reaction of mutant F279A, using 0.1 mM NADPH as coenzyme


proof_lines: reduction reaction of mutant W254A, using 0.1 mM NADPH as coenzyme


proof_lines: reduction reaction of mutant W125Y, using 0.1 mM NADPH as coenzyme


substrate_name: (R)-tetralol

substrate: (R)-tetralol


substrate_name: D-xylose

substrate: D-xylose


enzyme_display_name: biliverdin reductase

organism: Bos taurus

substrate_name: NADH

assay_conditions_summary: cofactor NADH

proof_lines:
without Cl-
with Cl-

enzyme: biliverdin reductase

substrate: NADH


proof_lines:
0.01 mM NADPH as substrate
0.1 mM biliverdin as substrate


substrate_name: biliverdin IXalpha

proof_lines:
cosubstrate NADPH
cosubstrate NADH

substrate: biliverdin IXalpha


organism: Rattus norvegicus

proof_lines: in the presence of 0.005 mM biliverdin, biliverdin reductase C280A


proof_lines: in the presence of 0.005 mM biliverdin, biliverdin reductase wild-type


substrate_name: Biliverdin

proof_lines: cosubstrate NADPH, biliverdin reductase C291A

proof_lines: cosubstrate NADH, biliverdin reductase C291A

substrate: Biliverdin


proof_lines: cosubstrate NADH, biliverdin reductase C280A

proof_lines: cosubstrate NADPH, biliverdin reductase C280A


proof_lines: in the presence of 0.005 mM biliverdin, biliverdin reductase C291A


proof_lines: mutant enzyme R171E, at pH 8.9, temperature not specified in the publication


proof_lines: mutant enzyme R171A, at pH 8.9, temperature not specified in the publication


proof_lines: mutant enzyme Y97F, at pH 8.9, temperature not specified in the publication


proof_lines: mutant enzyme R171K, at pH 8.9, temperature not specified in the publication


assay_conditions_summary: pH 8.9; cofactor NADPH

proof_lines: wild type enzyme, at pH 8.9, temperature not specified in the publication

proof_lines: cosubstrate NADPH, biliverdin reductase wild-type

proof_lines: cosubstrate NADH, biliverdin reductase wild-type


assay_conditions_summary: pH 7; cofactor NADPH

proof_lines:
cosubstrate NADPH
cosubstrate NADH
at pH 7


assay_conditions_summary: pH 7; cofactor NAD+


substrate_name: bilirubin

assay_conditions_summary: pH 7

substrate: bilirubin


substrate_name: NADP+

assay_conditions_summary: pH 8.7; cofactor NADP+

substrate: NADP+


enzyme_display_name: Delta(4)-3-oxosteroid 5beta-reductase

organism: Digitalis lanata

substrate_name: progesterone

enzyme: Delta(4)-3-oxosteroid 5beta-reductase

substrate: progesterone


proof_lines: pH 8.0, 40°C, recombinant mutant Y156V/N205M


proof_lines: pH 8.0, 40°C, recombinant wild-type enzyme


substrate_name: cortisol

substrate: cortisol


substrate_name: cortexone

substrate: cortexone


enzyme_display_name: maleylacetate reductase

organism: Pseudomonas aeruginosa

enzyme: maleylacetate reductase


substrate_name: maleylacetate

substrate: maleylacetate


smiles: CC(C(=O)C=CC(=O)O)C(=O)O


enzyme_display_name: protochlorophyllide reductase

organism: Cyanidioschyzon merolae

substrate_name: NADPH

proof_lines: pH and temperature not specified in the publication

enzyme: protochlorophyllide reductase

substrate: NADPH


substrate_name: protochlorophyllide

substrate: protochlorophyllide


organism: Daucus carota


enzyme_display_name: trans-2-enoyl-CoA reductase (NADPH)

organism: Escherichia coli

assay_conditions_summary: cofactor CoA

enzyme: trans-2-enoyl-CoA reductase (NADPH)


enzyme_display_name: 12-oxophytodienoate reductase

organism: Arabidopsis sp.

substrate_name: 12-oxo-cis-phythodienoic acid

enzyme: 12-oxophytodienoate reductase

substrate: 12-oxo-cis-phythodienoic acid


substrate_name: cyclohexenone

substrate: cyclohexenone


organism: Arabidopsis thaliana

proof_lines: pH 7.2, temperature not specified in the publication


substrate_name: Methyl vinyl ketone

smiles: CC(=O)C=C

substrate: Methyl vinyl ketone


smiles: CCCC=CC=O


substrate_name: (9S,13S)-12-oxophytodienoic acid

substrate: (9S,13S)-12-oxophytodienoic acid


organism: Solanum lycopersicum


enzyme_display_name: fumarate reductase (NADH)

organism: Leishmania major

substrate_name: Unknown

enzyme: fumarate reductase (NADH)

substrate: Unknown


enzyme_display_name: Delta(24)-sterol reductase

organism: Manduca sexta

enzyme: Delta(24)-sterol reductase


proof_lines:
desmosterol
pH 7.6, 37°C


substrate_name: desmosterol

substrate: desmosterol


enzyme_display_name: 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH)

organism: Rhodobacter capsulatus

substrate_name: 3,8-divinyl protochlorophyllide a

proof_lines: dimeric enzyme (low salt condition, 150 mM NaCl), at pH 8.0 and 25°C

proof_lines: dimeric enzyme (high salt condition, 1 M NaCl), at pH 8.0 and 25°C

substrate: 3,8-divinyl protochlorophyllide a


substrate_name: 3,8-divinyl chlorophyllide a

proof_lines: pH 8.0, 22°C, recombinant Strep II-tagged dimeric enzyme, low salt condition

proof_lines: pH 8.0, 22°C, recombinant Strep II-tagged dimeric enzyme, high salt condition

proof_lines: pH 8.0, 22°C, recombinant Strep II-tagged oligomeric enzyme, low salt condition

enzyme: 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH)

substrate: 3,8-divinyl chlorophyllide a


organism: Medicago truncatula

substrate_name: cyanidin

assay_conditions_summary: pH 6

substrate: cyanidin


substrate_name: delphinidin

substrate: delphinidin


substrate_name: pelargonidin

substrate: pelargonidin


assay_conditions_summary: pH 6; cofactor NADPH


substrate_name: NADH

assay_conditions_summary: pH 6; cofactor NADH

substrate: NADH


enzyme_display_name: crotonyl-CoA reductase

organism: Streptomyces collinus

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADPH

proof_lines: in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 1 mM dithioerythritol and 10% (v/v) glycerol, at 30°C

enzyme: crotonyl-CoA reductase


substrate_name: crotonyl-CoA

substrate: crotonyl-CoA


assay_conditions_summary: pH 6.5; 303.15 K; cofactor CoA

proof_lines: Ki above 0.4 mM, in 50 mM Tris/HCI pH 6.5 and 10% (v/v) glycerol, at 30°C


organism: uncultured bacterium

assay_conditions_summary: pH 5.5; 303.15 K; cofactor NADH


substrate_name: crotonoyl-CoA

assay_conditions_summary: pH 5.5; 303.15 K; cofactor CoA

substrate: crotonoyl-CoA


enzyme_display_name: fumarate reductase (cytochrome)

organism: Shewanella frigidimarina

substrate_name: fumarate

proof_lines: mutant enzyme H365A/H504A, at pH 6.0 and 25°C

proof_lines: mutant enzyme H365A/H504A, at pH 7.2 and 25°C

proof_lines: mutant enzyme H365A/H504A, at pH 7.5 and 25°C

enzyme: fumarate reductase (cytochrome)

substrate: fumarate


proof_lines: mutant enzyme H61M, with reduced methyl viologen as cosubstrate, at pH 9.0 and 25°C

proof_lines: mutant enzyme H61M, with reduced methyl viologen as cosubstrate, at pH 7.5 and 25°C

proof_lines: mutant enzyme H61M, with reduced methyl viologen as cosubstrate, at pH 6.0 and 25°C


proof_lines: mutant enzyme H61A, with reduced methyl viologen as cosubstrate, at pH 9.0 and 25°C

proof_lines: mutant enzyme H61A, with reduced methyl viologen as cosubstrate, at pH 7.5 and 25°C

proof_lines: mutant enzyme H61A, with reduced methyl viologen as cosubstrate, at pH 7.2 and 25°C


proof_lines: wild type enzyme, with reduced methyl viologen as cosubstrate, at pH 9.0 and 25°C

proof_lines: wild type enzyme, with reduced methyl viologen as cosubstrate, at pH 7.5 and 25°C


proof_lines: mutant enzyme Q363F/R402A, at pH 6.0 and 25°C

proof_lines: mutant enzyme Q363F/R402A, at pH 7.2 and 25°C

proof_lines: mutant enzyme Q363F/R402A, at pH 7.5 and 25°C


proof_lines:
mutant enzyme Q363F, at pH 6.0 and 25°C
mutant enzyme Q363F, at pH 7.2 and 25°C
mutant enzyme Q363F, at pH 7.5 and 25°C


substrate_name: succinate

proof_lines: with oxidized 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.8 and 25°C

proof_lines: with oxidized 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 25°C

proof_lines: with oxidized 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.5 and 25°C

substrate: succinate


proof_lines: with reduced methyl viologen as cosubstrate, at pH 8.0 and 25°C

proof_lines: with reduced methyl viologen as cosubstrate, at pH 7.6 and 25°C

proof_lines: with reduced methyl viologen as cosubstrate, at pH 7.2 and 25°C


enzyme_display_name: coproporphyrinogen oxidase

organism: Glycine max

substrate_name: Coproporphyrinogen-III

enzyme: coproporphyrinogen oxidase

substrate: Coproporphyrinogen-III


organism: Rattus sp.


substrate_name: coproporphyrinogen IV

substrate: coproporphyrinogen IV


substrate_name: Harderoporphyrinogen

substrate: Harderoporphyrinogen


substrate_name: pentacarboxylate porphyrinogen III

substrate: pentacarboxylate porphyrinogen III


enzyme_display_name: protoporphyrinogen oxidase

organism: Mus musculus

substrate_name: Mesoporphyrinogen-IX

enzyme: protoporphyrinogen oxidase

substrate: Mesoporphyrinogen-IX


substrate_name: protoporphyrinogen-IX

proof_lines: in detergent solution in presence of cardiolipin

substrate: protoporphyrinogen-IX


smiles: O=O


organism: Myxococcus xanthus


substrate_name: protoporphyrinogen IX

substrate: protoporphyrinogen IX


proof_lines: pH 8.1, 37°C, recombinant wild-type enzyme


enzyme_display_name: succinate dehydrogenase

organism: Corynebacterium glutamicum

enzyme: succinate dehydrogenase


substrate_name: Unknown

substrate: Unknown


organism: Mycolicibacterium phlei


organism: Rhodospirillum rubrum


substrate_name: phenazine methosulfate

substrate: phenazine methosulfate


organism: Vigna radiata


enzyme_display_name: dihydroorotate dehydrogenase (quinone)

substrate_name: S-dihydroorotate

enzyme: dihydroorotate dehydrogenase (quinone)

substrate: S-dihydroorotate


organism: Phytophthora infestans

substrate_name: (S)-dihydroorotate

substrate: (S)-dihydroorotate


substrate_name: coenzyme QD

substrate: coenzyme QD


organism: Staphylococcus aureus

substrate_name: FAD

assay_conditions_summary: pH 6.6; 303.15 K; cofactor FAD

substrate: FAD


proof_lines: pH and temperature not specified in the publication


enzyme_display_name: isovaleryl-CoA dehydrogenase

organism: Lathyrus oleraceus

substrate_name: isovaleryl-CoA

proof_lines:
purified enzyme
partially purified enzyme

enzyme: isovaleryl-CoA dehydrogenase

substrate: isovaleryl-CoA


substrate_name: n-valeryl-CoA

substrate: n-valeryl-CoA


organism: Homo sapiens

substrate_name: isobutyryl-CoA

assay_conditions_summary: 305.15 K; cofactor CoA

substrate: isobutyryl-CoA


substrate_name: hexanoyl-CoA

substrate: hexanoyl-CoA


enzyme_display_name: dihydroorotate oxidase (fumarate)

organism: Trypanosoma brucei

substrate_name: fumarate

enzyme: dihydroorotate oxidase (fumarate)

substrate: fumarate


enzyme_display_name: hydrogen peroxide-dependent heme synthase

organism: Listeria monocytogenes serotype 1/2a

substrate_name: Fe-coproporphyrin III

substrate: Fe-coproporphyrin III


enzyme: hydrogen peroxide-dependent heme synthase


smiles: OO

proof_lines: pH 7.0, temperature not specified in the publication


organism: Rhodobacter capsulatus

substrate_name: all-trans-zeta-carotene

substrate: all-trans-zeta-carotene


enzyme_display_name: alanine dehydrogenase

organism: Desulfovibrio desulfuricans

substrate_name: NADH

enzyme: alanine dehydrogenase

substrate: NADH


substrate_name: pyruvate

substrate: pyruvate


substrate_name: NAD+

assay_conditions_summary: cofactor NAD+

substrate: NAD+


substrate_name: L-Ala

smiles: CC(C(=O)O)N

substrate: L-Ala


organism: Shewanella sp.

assay_conditions_summary: pH 10; 283.15 K; cofactor NAD+


assay_conditions_summary: pH 10; 278.15 K; cofactor NADH


enzyme_display_name: diaminopimelate dehydrogenase

organism: Symbiobacterium thermophilum

substrate_name: phenylpyruvate

proof_lines: pH 8.5, 30°C, recombinant wild-type enzyme

enzyme: diaminopimelate dehydrogenase

substrate: phenylpyruvate


proof_lines: pH 8.5, 30°C, recombinant mutant T171S/R181F


proof_lines: pH 8.5, 30°C, recombinant mutant T171S/R181F/H227V


proof_lines: pH 8.5, 30°C, recombinant mutant T171S/H227V


proof_lines: pH 8.5, 30°C, recombinant mutant R181F/H227V


smiles: CCC(=O)C(=O)O


proof_lines: mutant R71E, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant R71I, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant R71Q, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant R71K, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant R71S, pH not specified in the publication, temperature not specified in the publication


smiles: C(CC(C(=O)O)N)CC(C(=O)O)N


proof_lines: pH 8.5, 30°C, recombinant mutant F146W/M152Q


enzyme_display_name: tryptophan dehydrogenase

organism: Nostoc punctiforme

substrate_name: L-tryptophan

enzyme: tryptophan dehydrogenase

substrate: L-tryptophan


proof_lines: mutant enzyme D168G, at pH 11.5 and 30°C


proof_lines: mutant enzyme I296N, at pH 11.5 and 30°C


proof_lines: mutant enzyme L59F/D168G/A234D/I296N, at pH 11.5 and 30°C


proof_lines: mutant enzyme A234D, at pH 11.5 and 30°C


assay_conditions_summary: pH 11; 298.15 K; cofactor NAD+


assay_conditions_summary: pH 7.5; 298.15 K; cofactor NAD+

proof_lines:
at pH 7.5 and 25°C
at pH 11.0 and 25°C


smiles: N

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADH


enzyme_display_name: glutamate dehydrogenase

organism: Agaricus bisporus

enzyme: glutamate dehydrogenase


assay_conditions_summary: cofactor NADH


substrate_name: L-glutamate

smiles: C(CC(=O)O)C(C(=O)O)N

substrate: L-glutamate


enzyme_display_name: valine dehydrogenase (NAD(+))

organism: Streptomyces fradiae

assay_conditions_summary: pH 10.4; 303.15 K; cofactor NADH

enzyme: valine dehydrogenase (NAD(+))


assay_conditions_summary: pH 10.4; 303.15 K; cofactor NAD+


enzyme_display_name: glutamate dehydrogenase [NAD(P)(+)]

organism: Rattus norvegicus

assay_conditions_summary: cofactor NADP+

proof_lines:
mitochondrial enzyme
enzyme from rough ER

enzyme: glutamate dehydrogenase [NAD(P)(+)]


substrate_name: NADP+

proof_lines:
enzyme from rough ER
mitochondrial enzyme

substrate: NADP+


substrate_name: glutamate

proof_lines:
mitochondrial enzyme, cofactor NAD+
enzyme from rough ER, cofactor NAD+
mitochondrial enzyme, cofactor NADP+

substrate: glutamate


organism: Xenopus laevis

proof_lines:
liver enzyme
kidney enzyme


proof_lines:
kidney enzyme
liver enzyme


substrate_name: Unknown

substrate: Unknown


enzyme_display_name: glutamate dehydrogenase (NADP(+))

organism: Bacillus sp. (in: firmicutes)

assay_conditions_summary: 303.15 K; cofactor NADP+

enzyme: glutamate dehydrogenase (NADP(+))


substrate_name: NADPH

assay_conditions_summary: 303.15 K; cofactor NADPH

substrate: NADPH


organism: Chlorella sorokiniana

assay_conditions_summary: pH 7; 311.65 K; cofactor NADP+

proof_lines:
pH 7.0, 38.5°C, alpha-homohexamer
pH 7.0, 38.5°C, beta-homohexamer
oxidative deamination


assay_conditions_summary: pH 7; 311.65 K; cofactor NADPH

proof_lines:
pH 7.0, 38.5°C, alpha-homohexamer
pH 7.0, 38.5°C, beta-homohexamer


proof_lines:
pH 7.0, 38.5°C, beta-homohexamer
oxidative deamination
pH 7.0, 38.5°C, alpha-homohexamer


enzyme_display_name: leucine dehydrogenase

organism: Geobacillus stearothermophilus

enzyme: leucine dehydrogenase


substrate_name: L-Leu

smiles: CC(C)CC(C(=O)O)N

substrate: L-Leu


enzyme_display_name: D-aspartate oxidase

organism: Caenorhabditis elegans

substrate_name: D-Aspartate

enzyme: D-aspartate oxidase

substrate: D-Aspartate


substrate_name: D-asparagine

assay_conditions_summary: pH 8.3; 310.15 K; buffer 40 mM sodium diphosphate

proof_lines: recombinant C47Ap protein, in 40 mM sodium diphosphate buffer (pH 8.3), at 37°C

substrate: D-asparagine


substrate_name: D-glutamate

substrate: D-glutamate


substrate_name: N-Methyl-D-aspartate

smiles: CNC(CC(=O)O)C(=O)O

substrate: N-Methyl-D-aspartate


substrate_name: N-methyl-D-asparagine

substrate: N-methyl-D-asparagine


substrate_name: D-histidine

substrate: D-histidine


substrate_name: D-proline

substrate: D-proline


substrate_name: D-Asp

substrate: D-Asp