substrate_name: sepiapterin

enzyme: sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming)

substrate: sepiapterin


enzyme_display_name: sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming)

organism: Equus caballus

substrate_name: NADP+

source_db: brenda

verification_status: unverified

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

assay_conditions_summary: pH 10.4; 298.15 K; cofactor NADP+

substrate: NADP+

status: unverified

source: brenda


organism: Homo sapiens

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution


substrate_name: menadione

substrate: menadione


substrate_name: Unknown

km_display: Not reported

ki_unit: mM

substrate: Unknown


substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

substrate: NADPH


enzyme_display_name: D-xylose 1-dehydrogenase (NADP(+), D-xylono-1,5-lactone-forming)

organism: Macaca fascicularis

substrate_name: D-xylose

assay_conditions_summary: pH 7.5; cofactor NADP+

proof_lines: oxidation reaction of mutant R37D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

proof_lines: oxidation reaction of mutant R37D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

substrate: D-xylose


proof_lines: oxidation reaction of mutant A36D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

proof_lines: oxidation reaction of mutant A36D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose

enzyme: D-xylose 1-dehydrogenase (NADP(+), D-xylono-1,5-lactone-forming)


proof_lines: oxidation reaction of mutant H76Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

proof_lines: oxidation reaction of mutant H76Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose


proof_lines: oxidation reaction of mutant H79Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

proof_lines: oxidation reaction of mutant H79Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose


proof_lines: oxidation reaction of mutant R41D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

proof_lines: oxidation reaction of mutant R41D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose


proof_lines: oxidation reaction of mutant R37A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

proof_lines: oxidation reaction of mutant R37A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose


proof_lines: oxidation reaction of mutant R41A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

proof_lines: oxidation reaction of mutant R41A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose


proof_lines: oxidation reaction of wild type enzyme, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose

proof_lines: oxidation reaction of wild type enzyme, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose


proof_lines: oxidation reaction of mutant F279A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose


proof_lines: oxidation reaction of mutant W254Y, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose


proof_lines: oxidation reaction of mutant F154A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose


proof_lines: oxidation reaction of mutant W125Y, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose


proof_lines: oxidation reaction of mutant W254A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose


enzyme_display_name: cinnamyl-alcohol dehydrogenase

organism: Eucalyptus gunnii

substrate_name: NAD+

assay_conditions_summary: cofactor NAD+

enzyme: cinnamyl-alcohol dehydrogenase

substrate: NAD+


assay_conditions_summary: cofactor NADP+


substrate_name: coniferyl alcohol

proof_lines:
with NADP+
with NAD+

substrate: coniferyl alcohol


proof_lines:
with NAD+
with NADP+
with NADH


organism: Hibiscus cannabinus

substrate_name: cinnamaldehyde

substrate: cinnamaldehyde


substrate_name: coniferyl aldehyde

substrate: coniferyl aldehyde


substrate_name: sinapyl aldehyde

substrate: sinapyl aldehyde


organism: Nicotiana tabacum

proof_lines:
pH 10.5, 30°C, recombinant CAD1-1
pH 8.8, 30°C, recombinant CAD1-7


proof_lines:
pH 6.25, 30°C, recombinant CAD1-1
pH 6.25, 30°C, recombinant CAD1-7


organism: Plagiochasma appendiculatum

proof_lines:
isoform CAD1, at pH 6.0 and 25°C
isoform CAD2, at pH 6.0 and 25°C


substrate_name: sinapaldehyde

proof_lines:
isoform CAD2, at pH 6.0 and 25°C
isoform CAD1, at pH 6.0 and 25°C

substrate: sinapaldehyde


substrate_name: coniferaldehyde

substrate: coniferaldehyde


enzyme_display_name: (+)-borneol dehydrogenase

organism: Salvia officinalis

substrate_name: (+)-borneol

enzyme: (+)-borneol dehydrogenase

substrate: (+)-borneol


substrate_name: (-)-Thujol

substrate: (-)-Thujol


enzyme_display_name: 1,3-propanediol dehydrogenase

organism: Limosilactobacillus reuteri

enzyme: 1,3-propanediol dehydrogenase


enzyme_display_name: uronate dehydrogenase

organism: Pseudomonas syringae pv. tomato

substrate_name: beta-D-galacturonate

assay_conditions_summary: pH 8; 298.15 K; buffer recombinant His-tagged enzyme

proof_lines: recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer

proof_lines: recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer

enzyme: uronate dehydrogenase

substrate: beta-D-galacturonate


assay_conditions_summary: pH 8; 298.15 K; buffer pH 8; cofactor NAD+


substrate_name: D-galacturonate

assay_conditions_summary: buffer recombinant His-tagged enzyme

substrate: D-galacturonate


enzyme_display_name: IMP dehydrogenase

organism: Burkholderia thailandensis

substrate_name: IMP

proof_lines: pH 8.0, 30°C, recombinant enzyme, with MgATP

proof_lines: pH 8.0, 30°C, recombinant enzyme, without MgATP

enzyme: IMP dehydrogenase

substrate: IMP


assay_conditions_summary: pH 8; 303.15 K; cofactor NAD+


organism: Eimeria tenella


proof_lines: at a fixed concentration of 0.5 mM NAD+ and 0.03 mM IMP


organism: Vigna unguiculata

substrate_name: inosine 5'-phosphate

substrate: inosine 5'-phosphate


assay_conditions_summary: pH 8.8; 303.15 K; cofactor NAD+


enzyme_display_name: tropinone reductase I

organism: Brugmansia arborea

proof_lines: pH 9.6, 22°C, recombinant His-tagged enzyme

enzyme: tropinone reductase I


substrate_name: tropinone

proof_lines: pH 6.4, 22°C, recombinant His-tagged enzyme

proof_lines: pH 6.4, temperature not specified in the publication

substrate: tropinone


substrate_name: Tropine

proof_lines: pH 9.6, temperature not specified in the publication

substrate: Tropine


organism: Cavia porcellus

substrate_name: testosterone

proof_lines:
membrane bound
solubilized with Triton X-100

substrate: testosterone


proof_lines:
solubilized with Triton X-100
membrane bound


organism: Mesocricetus auratus

substrate_name: farnesol

smiles: CC(=CCCC(=CCCC(=CCO)C)C)C

substrate: farnesol


substrate_name: geraniol

smiles: CC(=CCCC(=CCO)C)C

substrate: geraniol


substrate_name: NAD+

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NAD+

substrate: NAD+


substrate_name: cis-benzene dihydrodiol

substrate: cis-benzene dihydrodiol


substrate_name: geranylgeraniol

smiles: CC(=CCCC(=CCCC(=CCCC(=CCO)C)C)C)C

substrate: geranylgeraniol


substrate_name: beta-ionol

substrate: beta-ionol


substrate_name: NADP+

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADP+

proof_lines: pH 7.4, 25°C, with substrate S-camphorquinone

substrate: NADP+


substrate_name: NADH

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADH

substrate: NADH


substrate_name: glycochenodeoxycholic acid

substrate: glycochenodeoxycholic acid


substrate_name: lithocholic acid

substrate: lithocholic acid


substrate_name: glycolithocholic acid

substrate: glycolithocholic acid


organism: Oryctolagus cuniculus


proof_lines:
pH 7.4, 25°C
pH 7.4, 25°C, recombinant enzyme


substrate_name: alpha-tetralone

substrate: alpha-tetralone


substrate_name: Naphthalene dihydrodiol

substrate: Naphthalene dihydrodiol


substrate_name: benzene dihydrodiol

substrate: benzene dihydrodiol


smiles: CC(=O)C(=O)C

assay_conditions_summary: pH 7.4; 298.15 K; cofactor CoA


substrate_name: (S)-camphorquinone

substrate: (S)-camphorquinone


substrate_name: isatin

substrate: isatin


substrate_name: morphine

substrate: morphine


enzyme_display_name: methanol dehydrogenase

organism: Cupriavidus necator

substrate_name: methanol

smiles: CO

proof_lines: pH 9.5, 30°C, recombinant wild-type enzyme

enzyme: methanol dehydrogenase

substrate: methanol


assay_conditions_summary: pH 9.5; 303.15 K; cofactor NAD+


substrate_name: n-butanol

smiles: CCCCO

substrate: n-butanol


enzyme_display_name: mannitol dehydrogenase

organism: Apium graveolens

substrate_name: NAD+

assay_conditions_summary: pH 9.5; cofactor NAD+

proof_lines:
at pH 9.5
at pH 7.0

enzyme: mannitol dehydrogenase

substrate: NAD+


substrate_name: D-mannitol

smiles: C(C(C(C(C(CO)O)O)O)O)O

substrate: D-mannitol


enzyme_display_name: sn-glycerol-1-phosphate dehydrogenase

organism: Aeropyrum pernix

smiles: C(C(=O)COP(=O)(O)O)O

enzyme: sn-glycerol-1-phosphate dehydrogenase


substrate_name: NADPH

substrate: NADPH


substrate_name: dihydroxyacetone phosphate

assay_conditions_summary: cofactor NADH

proof_lines: mutant enzyme H271A, with NADH as coenzyme

substrate: dihydroxyacetone phosphate


proof_lines: mutant enzyme D144A, with NADPH as coenzyme

proof_lines: mutant enzyme D144A, with NADH as coenzyme


proof_lines: mutant enzyme H287A, with NADH as coenzyme


proof_lines: mutant enzyme D191N, with NADH as coenzyme


proof_lines: wild type enzyme, with NADPH as coenzyme


proof_lines: mutant enzyme D144N, with NADPH as coenzyme

proof_lines: mutant enzyme D144N, with NADH as coenzyme


assay_conditions_summary: pH 7; 338.15 K; cofactor NADPH


smiles: C(C(COP(=O)(O)O)O)O


assay_conditions_summary: pH 7; 338.15 K; cofactor NAD+


proof_lines: in the presence of 0.32 mM NADPH, pH 7.0, 65°C

proof_lines: in the presence of 0.32 mM NADH, pH 7.0, 65°C


assay_conditions_summary: pH 7; 338.15 K; cofactor NADH


enzyme_display_name: 2-(S)-hydroxypropyl-CoM dehydrogenase

organism: Xanthobacter autotrophicus

smiles: CC(CSCCS(=O)(=O)O)O

enzyme: 2-(S)-hydroxypropyl-CoM dehydrogenase


smiles: CCC(=O)C


smiles: CCC(C)O


smiles: CC(C)O


smiles: CC(=O)CSCCS(=O)(=O)O


substrate_name: Unknown

substrate: Unknown


organism: Ensifer adhaerens

assay_conditions_summary: pH 7.5; cofactor NADPH

proof_lines: pH 7.5, mutant enzyme D176A, cofactor: NADPH


assay_conditions_summary: pH 8; cofactor NADPH

proof_lines: pH 8.0, mutant enzyme H180A, cofactor: NADPH


assay_conditions_summary: pH 6.5; cofactor NADPH

proof_lines: pH 6.5, mutant enzyme K94G, cofactor: NADPH


proof_lines: pH 6.5, mutant enzyme S10G/A13G, cofactor: NADH

proof_lines: pH 6.5, mutant enzyme S10G/A13G, cofactor: NADPH


proof_lines: pH 6.5, mutant enzyme S33D/A13G, cofactor: NADPH

proof_lines: pH 6.5, mutant enzyme S33D/A13G, cofactor: NADH


proof_lines: pH 6.5, mutant enzyme A13G, cofactor: NADH

proof_lines: pH 6.5, mutant enzyme A13G, cofactor: NADPH


proof_lines: pH 6.5, mutant enzyme S10G, cofactor: NADPH


proof_lines: pH 6.5 recombinant wild-type enzyme, cofactor: NADPH

proof_lines: pH 6.5, native wild-type enzyme, cofactor: NADPH


proof_lines: pH 6.5, mutant enzyme G206I, cofactor: NADPH


proof_lines:
pH 6.5 recombinant wild-type enzyme
pH 6.5, native wild-type enzyme


substrate_name: NADH

assay_conditions_summary: pH 6.5; cofactor NADH

substrate: NADH


assay_conditions_summary: pH 6.5; 303.15 K; cofactor NADH

proof_lines: mutant enzyme A13G/S33D, at pH 6.5 and 30°C


substrate_name: D-glucosone

smiles: C(C(C(C(C(=O)C=O)O)O)O)O

substrate: D-glucosone


assay_conditions_summary: pH 6.5; 303.15 K; cofactor NADPH


proof_lines: mutant enzyme A13G/S10G, at pH 6.5 and 30°C


enzyme_display_name: sulfopropanediol 3-dehydrogenase

organism: Cupriavidus pinatubonensis

assay_conditions_summary: pH 9; 303.15 K; cofactor NAD+

enzyme: sulfopropanediol 3-dehydrogenase


enzyme_display_name: sulfoacetaldehyde reductase (NADPH)

organism: Chromohalobacter israelensis

assay_conditions_summary: pH 9; 296.15 K; buffer 0.02 mMTris/H2SO4; cofactor NADPH

proof_lines: in 0.02 mMTris/H2SO4 buffer, pH 9.0, at 23°C

enzyme: sulfoacetaldehyde reductase (NADPH)


assay_conditions_summary: pH 9; 296.15 K; buffer 0.02 mMTris/H2SO4


organism: Klebsiella oxytoca

substrate_name: isethionate

substrate: isethionate


substrate_name: NADP+

assay_conditions_summary: pH 10; 295.15 K; cofactor NADP+

substrate: NADP+


enzyme_display_name: 11-cis-retinol dehydrogenase

organism: Homo sapiens

substrate_name: all-trans retinol

enzyme: 11-cis-retinol dehydrogenase

substrate: all-trans retinol


assay_conditions_summary: pH 7.4; 310.15 K; cofactor NADPH


assay_conditions_summary: pH 7.4; 310.15 K; cofactor NADP+


assay_conditions_summary: pH 7.4; 310.15 K; cofactor NAD+


assay_conditions_summary: pH 7.4; 310.15 K; cofactor NADH


substrate_name: all-trans retinal

substrate: all-trans retinal


proof_lines:
pH 7.4, 37°C
microsomal preparations of RDH10


assay_conditions_summary: pH 8; 310.15 K; cofactor NAD+

proof_lines: microsomal preparations of RDH10, cofactor: NAD+


substrate_name: 11-cis-retinol-[cellular retinaldehyde binding protein]


proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication

substrate: 11-cis-retinol-[cellular retinaldehyde binding protein]


substrate_name: all-trans-retinal

substrate: all-trans-retinal


enzyme_display_name: eugenol synthase

organism: Clarkia breweri

substrate_name: coniferyl acetate

proof_lines:
CbEGS2, pH 6.5, 28°C
CbEGS1, pH 6.5, 28°C

enzyme: eugenol synthase

substrate: coniferyl acetate


enzyme_display_name: secoisolariciresinol dehydrogenase

organism: Dysosma pleiantha

substrate_name: (-)-secoisolariciresinol

proof_lines: pH 8.8, 20°C, recombinant His-tagged enzyme

enzyme: secoisolariciresinol dehydrogenase

substrate: (-)-secoisolariciresinol


enzyme_display_name: phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating)

organism: Neomoorella thermoacetica

assay_conditions_summary: pH 7.5; 323.15 K; cofactor NAD+

proof_lines: mutant enzyme A30D/R31I/T32I, in 100 mM HEPES (pH 7.5), at 50°C


proof_lines: mutant enzyme A30C/R31I/T32K, in 100 mM HEPES (pH 7.5), at 50°C

enzyme: phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating)


proof_lines: mutant enzyme R31T, in 100 mM HEPES (pH 7.5), at 50°C


proof_lines: mutant enzyme A30E/R31I/T32D, in 100 mM HEPES (pH 7.5), at 50°C


proof_lines: wild type enzyme, in 100 mM HEPES (pH 7.5), at 50°C


proof_lines: mutant enzyme R31I/T32K, in 100 mM HEPES (pH 7.5), at 50°C


proof_lines: mutant enzyme R31I, in 100 mM HEPES (pH 7.5), at 50°C


enzyme_display_name: acetoacetyl-CoA reductase

organism: Candidatus Accumulibacter phosphatis

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

enzyme: acetoacetyl-CoA reductase


substrate_name: acetoacetyl-CoA

assay_conditions_summary: pH 7.5; 298.15 K; cofactor CoA

proof_lines: recombinant enzyme, with NADPH as cosubstrate, at pH 7.5 and 25°C

proof_lines: recombinant enzyme, with NADH as cosubstrate, at pH 7.5 and 25°C

substrate: acetoacetyl-CoA


assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADH


enzyme_display_name: (S)-malate dehydrogenase (NAD(+), oxaloacetate-forming)

organism: Flavobacterium frigidimaris

assay_conditions_summary: pH 10; 303.15 K; cofactor NAD+


substrate_name: (S)-malate

enzyme: (S)-malate dehydrogenase (NAD(+), oxaloacetate-forming)

substrate: (S)-malate


organism: Malassezia pachydermatis

substrate_name: oxaloacetate

assay_conditions_summary: pH 7.6; buffer note "in 50 mM Tris-HCl

proof_lines: in 50 mM Tris-HCl buffer (pH 7.6), 1 mM MgCl2

substrate: oxaloacetate


organism: Tritrichomonas suis

assay_conditions_summary: cofactor NADH


enzyme_display_name: L-arabinose 1-dehydrogenase [NAD(P)(+)]

organism: Rhizobium leguminosarum bv. trifolii

substrate_name: L-arabinose

assay_conditions_summary: pH 9; 296.15 K; cofactor NADP+

proof_lines: with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C

proof_lines: with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C

enzyme: L-arabinose 1-dehydrogenase [NAD(P)(+)]

substrate: L-arabinose


proof_lines: L-arabinose as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C


substrate_name: D-galactose

substrate: D-galactose


substrate_name: NAD+

assay_conditions_summary: pH 9; 296.15 K; cofactor NAD+

substrate: NAD+


substrate_name: D-fucose

smiles: CC(C(C(C(C=O)O)O)O)O

substrate: D-fucose


organism: Haemophilus influenzae

substrate_name: D-ribulose 5-phosphate

smiles: C(C(C(C(=O)CO)O)O)OP(=O)(O)O

substrate: D-ribulose 5-phosphate


substrate_name: D-arabinose 5-phosphate

smiles: C(C(C(C(C=O)O)O)O)OP(=O)(O)O

substrate: D-arabinose 5-phosphate


substrate_name: D-ribulose

smiles: C(C(C(C(=O)CO)O)O)O

substrate: D-ribulose


substrate_name: D-xylulose 5-phosphate

substrate: D-xylulose 5-phosphate


substrate_name: D-ribose 5-phosphate

substrate: D-ribose 5-phosphate


substrate_name: NADPH

assay_conditions_summary: pH 7.2; 298.15 K; cofactor NADPH

substrate: NADPH


substrate_name: NADH

assay_conditions_summary: pH 7.2; 298.15 K; cofactor NADH

substrate: NADH


assay_conditions_summary: pH 7.1; 303.15 K; cofactor NADPH


substrate_name: ribulose 5-phosphate

substrate: ribulose 5-phosphate


substrate_name: D-ribitol 5-phosphate

smiles: C(C(C(C(COP(=O)(O)O)O)O)O)O

proof_lines:
presence of CTP, pH 7.1, 30°C
pH 7.1, 30°C

substrate: D-ribitol 5-phosphate


organism: Arabidopsis thaliana

proof_lines: mutant enzyme S133A, pH and temperature not specified in the publication


proof_lines: wild type enzyme, pH and temperature not specified in the publication


proof_lines: mutant enzyme S133T, pH and temperature not specified in the publication


enzyme_display_name: isocitrate dehydrogenase (NADP(+))

organism: Psychromonas marina

substrate_name: isocitrate

enzyme: isocitrate dehydrogenase (NADP(+))

substrate: isocitrate


organism: Thermoplasma acidophilum

substrate_name: NADP+

assay_conditions_summary: pH 7.5; 333.15 K; cofactor NADP+

substrate: NADP+


enzyme_display_name: phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating)

organism: Arthrobacter globiformis

assay_conditions_summary: cofactor NADP+


enzyme: phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating)


organism: Neurospora crassa

proof_lines: wild-type, mutant col 10, heterocaryon col-10-wild-type


proof_lines:
heterocaryon col-3-wild-type
heterocaryon col-10-wild-type


proof_lines:
heterocaryon col-3-col-10
source of enzyme: peak I
source of enzyme: whole cells


organism: Ovis aries

assay_conditions_summary: cofactor NADPH


proof_lines: 50 mM Tris-acetate, 5-10 mM MgCl2, pH 7.7


assay_conditions_summary: pH 7.7; 296.65 K; cofactor NADP+

proof_lines:
10 mM Tris-acetate, 10 mM MgCl2, pH 7.7
10 mM Tris-acetate, 80 mM KCl, pH 7.7
50 mM Tris-acetate, pH 7.7


organism: Synechococcus sp.


enzyme_display_name: polyprenol dehydrogenase [NAD(P)H]

organism: Homo sapiens

assay_conditions_summary: pH 8; 310.15 K; cofactor NAD+

proof_lines: pH 8.0, 37°C, cosubstrate: ditrans,polycis-polyprenol

enzyme: polyprenol dehydrogenase [NAD(P)H]


assay_conditions_summary: pH 8; 310.15 K; cofactor NADP+


substrate_name: ditrans,polycis-polyprenol

assay_conditions_summary: pH 8; 310.15 K; cofactor NADPH

proof_lines:
pH 8.0, 37°C, cosubstrate: NAD+
pH 8.0, 37°C, cosubstrate: NADPH

substrate: ditrans,polycis-polyprenol


assay_conditions_summary: pH 8; 310.15 K; cofactor NADH


enzyme_display_name: glucose 1-dehydrogenase [NAD(P)(+)]

organism: Gluconobacter oxydans

assay_conditions_summary: pH 8; 298.15 K; cofactor NADP+

enzyme: glucose 1-dehydrogenase [NAD(P)(+)]


substrate_name: D-glucose

substrate: D-glucose


organism: Pseudomonas sp.

assay_conditions_summary: cofactor NAD+


enzyme_display_name: pyridoxine 4-dehydrogenase

organism: Schizosaccharomyces pombe

enzyme: pyridoxine 4-dehydrogenase


substrate_name: pyridoxal

substrate: pyridoxal


enzyme_display_name: mannitol 2-dehydrogenase

organism: Cereibacter sphaeroides

enzyme: mannitol 2-dehydrogenase


substrate_name: D-mannitol

substrate: D-mannitol


substrate_name: NAD+

substrate: NAD+


substrate_name: D-arabinitol

smiles: C(C(C(C(CO)O)O)O)O

substrate: D-arabinitol


substrate_name: D-fructose

substrate: D-fructose


enzyme_display_name: gluconate 5-dehydrogenase

organism: Aspergillus niger

assay_conditions_summary: pH 7

proof_lines: pH 7, temperature not specified in the publication

proof_lines: pH 7.0, temperature not specified in the publication

enzyme: gluconate 5-dehydrogenase


substrate_name: D-gluconate

assay_conditions_summary: pH 8

proof_lines: pH 8, temperature not specified in the publication

proof_lines: pH 8.0, temperature not specified in the publication

substrate: D-gluconate


enzyme_display_name: glyoxylate reductase (NADP(+))

organism: Chlamydomonas reinhardtii

substrate_name: glyoxylate

enzyme: glyoxylate reductase (NADP(+))

substrate: glyoxylate


enzyme_display_name: hydroxypyruvate reductase

substrate_name: phenylpyruvate

proof_lines:
pH 7, 30°C
pH 7.0, 30°C, recombinant enzyme

enzyme: hydroxypyruvate reductase

substrate: phenylpyruvate


substrate_name: Hydroxypyruvate

proof_lines:
pH 7, 30°C
pH 7.0, 30°C, recombinant enzyme
GLYR1, at pH 7.8 and 22°C

substrate: Hydroxypyruvate


enzyme_display_name: ketol-acid reductoisomerase (NADP(+))

organism: Hordeum vulgare

substrate_name: NADPH

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

enzyme: ketol-acid reductoisomerase (NADP(+))

substrate: NADPH


substrate_name: Unknown

proof_lines: pH 7.5, 25°C, with 2-aceto-2-hydroxybutyrate as substrate

proof_lines: pH 7.5, 25°C, with 2-acetolactate as substrate

substrate: Unknown


organism: Meiothermus ruber

assay_conditions_summary: pH 7; 323.15 K; cofactor NADPH

proof_lines: wild type enzyme, with NADPH as cosubstrate, at pH 7.0 and 50°C

proof_lines: wild type enzyme, with NADH as cosubstrate, at pH 7.0 and 50°C


proof_lines: mutant enzyme T84S, with NADH as cosubstrate, at pH 7.0 and 50°C

proof_lines: mutant enzyme T84S, with NADPH as cosubstrate, at pH 7.0 and 50°C


organism: Streptomyces coelicolor


substrate_name: pyruvate

substrate: pyruvate


organism: Streptomyces griseus


organism: Streptomyces pristinaespiralis


organism: Vigna radiata var. radiata

smiles: CCC(C(=O)C)(C(=O)O)O


enzyme_display_name: hydroxymethylglutaryl-CoA reductase

organism: Delftia acidovorans

substrate_name: NADH

assay_conditions_summary: pH 7.4; cofactor NADH

proof_lines: at pH 7.4, temperature not specified in the publication

enzyme: hydroxymethylglutaryl-CoA reductase

substrate: NADH


assay_conditions_summary: pH 7.4; cofactor CoA


enzyme_display_name: phosphoglycerate dehydrogenase

organism: Beta vulgaris

enzyme: phosphoglycerate dehydrogenase


organism: Marchantia polymorpha

assay_conditions_summary: pH 9

proof_lines: pH 9.0, temperature not specified in the publication


assay_conditions_summary: pH 9; cofactor NAD+


enzyme_display_name: L-lactate dehydrogenase (cytochrome)

organism: Pseudomonas aeruginosa

substrate_name: L-lactate

proof_lines: pH 7.4, temperature not specified in the publication

enzyme: L-lactate dehydrogenase (cytochrome)

substrate: L-lactate


organism: Wickerhamomyces anomalus


enzyme_display_name: methanol dehydrogenase (cytochrome c)

organism: Hyphomicrobium denitrificans

substrate_name: ferricytochrome cL

proof_lines: pH 5.5, ionic strength: 0.0160 M, steady-state kinetic parameter

proof_lines: pH 6.0, ionic strength: 0.0172 M, steady-state kinetic parameter

proof_lines: pH 6.5, ionic strength: 0.0183 M, steady-state kinetic parameter

enzyme: methanol dehydrogenase (cytochrome c)

substrate: ferricytochrome cL


substrate_name: methanol

smiles: CO

substrate: methanol


enzyme_display_name: pyranose oxidase

organism: Phlebiopsis gigantea

substrate_name: D-glucose

proof_lines: pH 7.3, 22°C, recombinant wild-type enzyme

enzyme: pyranose oxidase

substrate: D-glucose


smiles: C(C=O)C(C(C(CO)O)O)O


substrate_name: D-xylose

substrate: D-xylose


substrate_name: L-sorbose

substrate: L-sorbose


substrate_name: methyl-beta-D-glucoside

substrate: methyl-beta-D-glucoside


substrate_name: oxygen

smiles: O=O

substrate: oxygen


enzyme_display_name: alcohol oxidase

organism: Komagataella pastoris

proof_lines:
methanol concentration 1 mM
methanol concentration 10 mM
methanol concentration 100 mM

enzyme: alcohol oxidase


substrate_name: ethanol

smiles: CCO

substrate: ethanol


substrate_name: n-Propanol

smiles: CCCO

substrate: n-Propanol


substrate_name: formaldehyde

smiles: C=O

substrate: formaldehyde


substrate_name: n-butanol

smiles: CCCCO

substrate: n-butanol


substrate_name: ethylene glycol

smiles: C(CO)O

substrate: ethylene glycol


smiles: CC(C)O


proof_lines:
at pH 7.4 and 30°C
pH 6.5, 22°C, immobilized enzyme
oxygen concentration 0.19 mM


enzyme_display_name: choline oxidase

organism: Arthrobacter globiformis

substrate_name: Betaine aldehyde

enzyme: choline oxidase

substrate: Betaine aldehyde


proof_lines: mutant enzyme S101A, in 50 mM sodium diphosphate, pH 10.0, at 25°C


substrate_name: choline

smiles: C[N+](C)(C)CCO

substrate: choline


proof_lines:
pH 7.0, 25°C, mutant N510A
pH 10.0, 25°C, mutant N510A


proof_lines:
pH 7.0, 25°C, mutant N510H
pH 10.0, 25°C, mutant N510H


proof_lines:
pH 7.0, 25°C, wild-type enzyme
wild-type, 30°C, pH 8
wild type enzyme, at pH 7.0 and 25°C


smiles: CC(C)(C)CCO


proof_lines:
pH 5.5, 25°C, mutant H466A
pH 5.5, 25°C, recombinant mutant H466A
pH 6.0, 25°C, recombinant mutant H466A


proof_lines: mutant enzyme H351Q, in 50 mM potassium phosphate (pH 7.0), at 25°C


proof_lines: wild type enzyme, in 50 mM potassium phosphate (pH 7.0), at 25°C


substrate_name: betaine-aldehyde

substrate: betaine-aldehyde


proof_lines: previously stored at pH 6 and -20°C, measured at pH 7 and 25°C

proof_lines: previously stored at pH 6 and 0°C, measured at pH 7 and 25°C


proof_lines: wild type enzyme, in 50 mM sodium diphosphate, pH 10.0, at 25°C


proof_lines: apparent, H99N mutant, substrate choline, pH 7, 25°C. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system


proof_lines: pH 10, 25°C, cosubstrate betaine aldehyde


substrate_name: N-Methylethanolamine

smiles: CNCCO

substrate: N-Methylethanolamine


substrate_name: N,N-dimethylethanolamine

smiles: CN(C)CCO

substrate: N,N-dimethylethanolamine


substrate_name: Unknown

substrate: Unknown


proof_lines: mutant enzyme M62A/F357A, at pH 7.0 and 25°C


enzyme_display_name: long-chain-alcohol oxidase

organism: Lotus japonicus

proof_lines: pH and temperature not specified in the publication

enzyme: long-chain-alcohol oxidase


smiles: CCCCCCCCCCCCCCCCO

proof_lines: 1 mM, purified active LjFAO1 protein, compared to substrate preferences with the AtFAO3 gene of Arabidopsis thaliana


smiles: C(CCCCCCCCO)CCCCCCCO


smiles: CCCCCCCCCCCCO

proof_lines: 5 mM, purified active LjFAO1 protein, compared to substrate preferences with the AtFAO3 gene of Arabidopsis thaliana


organism: [Candida] cloacae

substrate_name: Dodecanol

substrate: Dodecanol


enzyme_display_name: glucose oxidase

organism: Cladosporium neopsychrotolerans

substrate_name: beta-D-glucose

enzyme: glucose oxidase

substrate: beta-D-glucose


proof_lines: pH 7.0, 20-25°C, mutant enzyme Y169C/A211C


enzyme_display_name: aryl-alcohol oxidase

organism: Pleurotus eryngii

substrate_name: benzyl alcohol

proof_lines: mutant F501A expressed in Emericella nidulans

enzyme: aryl-alcohol oxidase

substrate: benzyl alcohol


substrate_name: 3-anisyl alcohol

substrate: 3-anisyl alcohol


smiles: CC=CC=CCO


substrate_name: 4-anisyl alcohol

substrate: 4-anisyl alcohol


substrate_name: veratryl alcohol

substrate: veratryl alcohol


proof_lines:
pH 6.0, 24°C, wild-type enzyme
pH 8.0, 25°C, wild-type enzyme


proof_lines: mutant F501Y expressed in Emericella nidulans


proof_lines: wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0


substrate_name: 3-fluorobenzyl alcohol

substrate: 3-fluorobenzyl alcohol


proof_lines: pH 8.0, 25°C, recombinant enzyme from Escherichia coli


substrate_name: 3-Chlorobenzyl alcohol

substrate: 3-Chlorobenzyl alcohol


substrate_name: 4-fluorobenzyl alcohol

substrate: 4-fluorobenzyl alcohol


substrate_name: 3-chloro-4-anisyl alcohol

proof_lines: pH 8.0, 25°C, recombinant enzyme from Emericella nidulans

substrate: 3-chloro-4-anisyl alcohol


substrate_name: 4-Chlorobenzyl alcohol

substrate: 4-Chlorobenzyl alcohol


substrate_name: cinnamyl alcohol

substrate: cinnamyl alcohol


substrate_name: isovanillyl alcohol

substrate: isovanillyl alcohol


substrate_name: 4-methoxybenzyl alcohol

substrate: 4-methoxybenzyl alcohol


proof_lines:
pH 6.0, 25°C, mutant H502S
pH 60, 25°C, mutant H502S


proof_lines:
pH 6.0, 25°C, mutant H502A
pH 60, 25°C, mutant H502A


proof_lines:
pH 6.0, 25°C, mutant H546A
pH 60, 25°C, mutant H546A


proof_lines:
pH 6.0, 25°C, mutant H546S
pH 60, 25°C, mutant H546S


proof_lines: recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C

proof_lines: recombinant wild-type enzyme, pH 6.0, 24°C


proof_lines: recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C


proof_lines: recombinant protein from wild-type Pichia pastoris, pH 6, 25°C


proof_lines: pH 6.0, 25°C, wild-type enzyme, overall reaction


proof_lines:
pH 6, 25°C, presence of 1 mM H2O2
pH 6, 25°C, presence of 15 mM H2O2
pH 6, 25°C


proof_lines: pH 6, 25°C, presence of 6 mM formylfurancarboxylic acid

proof_lines: pH 6, 25°C, presence of 0.8 mM formylfurancarboxylic acid

proof_lines: substrate alpha-deuterated 4-methoxybenzyl alcohol, pH 6.0, 25°C


proof_lines: with 3-fluorobenzyl alcohol, 25°C, pH 6.0, recombinant enzyme

proof_lines: with 2,4-hexadien-1-ol, 25°C, pH 6.0, recombinant enzyme

proof_lines: with 4-anisyl alcohol, 25°C, pH 6.0, recombinant enzyme


substrate_name: 2,4-Dimethoxybenzyl alcohol

substrate: 2,4-Dimethoxybenzyl alcohol


proof_lines: mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0


substrate_name: veratraldehyde

proof_lines: wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0

substrate: veratraldehyde


smiles: CC=CC=CC=O

proof_lines: wild type enzyme, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0


substrate_name: benzaldehyde

substrate: benzaldehyde


proof_lines: recombinant H91N FX7 mutant, pH 6.0, 24°C


proof_lines: pH 6.0, 25°C, mutant F501A, overall reaction

proof_lines: mutant F501A expressed in Emericella nidulans

proof_lines: pH 6.0, 25°C, mutant F501A, oxidative half-reaction


proof_lines: pH 6.0, 25°C, mutant F501W, overall reaction

proof_lines: pH 6.0, 25°C, mutant F501W, oxidative half-reaction


proof_lines: pH 6.0, 25°C, mutant F501Y, overall reaction

proof_lines: mutant F501Y expressed in Emericella nidulans

proof_lines: pH 6.0, 25°C, mutant F501Y, oxidative half-reaction


enzyme_display_name: L-gulonolactone oxidase

organism: Capra hircus

enzyme: L-gulonolactone oxidase


enzyme_display_name: galactose oxidase

organism: Aspergillus sp.

enzyme: galactose oxidase


substrate_name: D-galactose

substrate: D-galactose


organism: Fusarium subglutinans


enzyme_display_name: glycerol-3-phosphate dehydrogenase

organism: Cyberlindnera jadinii

substrate_name: DL-alpha-glycerophosphate

smiles: C(C(COP(=O)(O)O)O)O

enzyme: glycerol-3-phosphate dehydrogenase

substrate: DL-alpha-glycerophosphate


organism: Mus musculus

substrate_name: Unknown

proof_lines: pH not specified in the publication, 37°C

substrate: Unknown


enzyme_display_name: malate dehydrogenase (quinone)

organism: Mycolicibacterium smegmatis

enzyme: malate dehydrogenase (quinone)


substrate_name: (S)-malate

substrate: (S)-malate


enzyme_display_name: alcohol dehydrogenase (quinone)

organism: Gluconobacter oxydans

substrate_name: pyrroloquinoline quinone

proof_lines: pH 5.0, 25°C, quinone-bound enzyme, in presence of N-dodecyl-beta-D-maltoside

proof_lines: ubiquinone-bound enzyme, in the presence of dodecylmaltoside, at pH 5.0 and 25°C

proof_lines: pH 5.0, 25°C, quinone-free enzyme, in presence of N-dodecyl-beta-D-maltoside

enzyme: alcohol dehydrogenase (quinone)

substrate: pyrroloquinoline quinone


organism: Gluconobacter sp.

substrate_name: ethanol

substrate: ethanol


substrate_name: acetaldehyde

smiles: CC=O

substrate: acetaldehyde


organism: Acinetobacter calcoaceticus

substrate_name: quinate

proof_lines: pH 6.5, 25°C,with potassium ferricyanide or a combination of phenazine methosulfate and 2,6-dichlorophenol indophenol as electron acceptor

substrate: quinate


substrate_name: shikimate

proof_lines: pH 6.5, 25°C, with potassium ferricyanide or a combination of phenazine methosulfate and 2,6-dichlorophenol indophenol as electron acceptor

substrate: shikimate


substrate_name: potassium ferricyanide

substrate: potassium ferricyanide


substrate_name: 2,6-dichlorophenol indophenol

substrate: 2,6-dichlorophenol indophenol


proof_lines: pH 8.0, temperature not specified in the publication, recombinant enzyme


substrate_name: oxidized coenzyme F420

substrate: oxidized coenzyme F420


substrate_name: D-glucose 6-phosphate

smiles: C(C(C(C(C(C=O)O)O)O)O)OP(=O)(O)O

substrate: D-glucose 6-phosphate


enzyme_display_name: choline dehydrogenase

organism: Rattus norvegicus

substrate_name: phenazine methosulfate

proof_lines:
at 10 mM choline
with choline

enzyme: choline dehydrogenase

substrate: phenazine methosulfate


substrate_name: p-benzoquinone

substrate: p-benzoquinone


substrate_name: Betaine aldehyde

substrate: Betaine aldehyde


proof_lines: inhibits the oxidation of choline competitively


substrate_name: choline

proof_lines: electron acceptor: phenazine methosulfate

substrate: choline


enzyme_display_name: cellobiose dehydrogenase (acceptor)

organism: Hypoxylon haematostroma

substrate_name: D-glucose

enzyme: cellobiose dehydrogenase (acceptor)

substrate: D-glucose


substrate_name: cytochrome c

substrate: cytochrome c


substrate_name: maltose

substrate: maltose


organism: Stachybotrys bisbyi

proof_lines:
at pH 5.0 and 30°C
at pH 5.5 and 30°C


proof_lines:
at pH 5.0 and 30°C
at pH 5.5 and 30°C
at pH 5.?5 and 30°C


organism: Trametes cinnabarina

substrate_name: cellobiose

substrate: cellobiose


enzyme_display_name: L-2-hydroxyglutarate dehydrogenase

substrate_name: NAD+

assay_conditions_summary: pH 8.7; 310.15 K; cofactor NAD+

enzyme: L-2-hydroxyglutarate dehydrogenase

substrate: NAD+


smiles: C(CC(=O)O)C(C(=O)O)O


enzyme_display_name: glycerol dehydrogenase (acceptor)

organism: Gluconobacter thailandicus

substrate_name: D-ribonate

enzyme: glycerol dehydrogenase (acceptor)

substrate: D-ribonate


substrate_name: D-arabonate

smiles: C(C(C(C(C(=O)O)O)O)O)O

substrate: D-arabonate


enzyme_display_name: hydroxyacid-oxoacid transhydrogenase

organism: Homo sapiens

proof_lines: +/-0.012 mM, in overepressed HEK cells, in presence of 0.5 mM 2-oxoglutarate

enzyme: hydroxyacid-oxoacid transhydrogenase


proof_lines: +/-0.009 mM, in overexpressed HEK cells, in presence of 0.1 mM 4-hydroxybutyrate


enzyme_display_name: L-sorbose 1-dehydrogenase

substrate_name: L-sorbose

proof_lines: electron acceptor 2,6-dichloroindophenol, pH 7.0, 25°C

enzyme: L-sorbose 1-dehydrogenase

substrate: L-sorbose


enzyme_display_name: hydrogen dehydrogenase (NADP(+))

organism: Pyrococcus furiosus

smiles: [HH]

proof_lines:
cosubstrate: benzyl viologen
cosubstrate: methyl viologen

enzyme: hydrogen dehydrogenase (NADP(+))


substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

substrate: NADP+


substrate_name: NADPH

assay_conditions_summary: pH 8.4; 353.15 K; cofactor NADPH

proof_lines:
80°C, pH 8.4, recombinant enzyme
80°C, pH 8.4, native enzyme

substrate: NADPH


enzyme_display_name: hydrogen:quinone oxidoreductase

proof_lines: pH 8.0, temperature not specified in the publication

enzyme: hydrogen:quinone oxidoreductase


enzyme_display_name: 2,3-dihydroxybenzoate 3,4-dioxygenase

organism: Pseudomonas fluorescens

enzyme: 2,3-dihydroxybenzoate 3,4-dioxygenase


enzyme_display_name: cysteine dioxygenase

organism: Cupriavidus necator

substrate_name: L-cysteine

smiles: C(C(C(=O)O)N)S

proof_lines: pH 6.5, 30°C, recombinant His-tagged enzyme

enzyme: cysteine dioxygenase

substrate: L-cysteine


enzyme_display_name: protocatechuate 3,4-dioxygenase

organism: Stenotrophomonas maltophilia

enzyme: protocatechuate 3,4-dioxygenase


enzyme_display_name: arachidonate 12-lipoxygenase

organism: Oryctolagus cuniculus

substrate_name: linoleic acid

smiles: CCCCCC=CCC=CCCCCCCCC(=O)O

enzyme: arachidonate 12-lipoxygenase

substrate: linoleic acid


proof_lines:
pH 7.4, 20?, wild-type
pH 7.4, 20°C, wild-type


substrate_name: arachidonic acid

smiles: CCCCCC=CCC=CCC=CCC=CCCCC(=O)O

substrate: arachidonic acid


substrate_name: methyl arachidonate

smiles: CCCCCC=CCC=CCC=CCC=CCCCC(=O)OC

substrate: methyl arachidonate


smiles: O=O

proof_lines: pH 7.4, wild-type enzyme, with substrate linoleic acid


enzyme_display_name: arachidonate 5-lipoxygenase

organism: Cavia porcellus

substrate_name: 5,8,11,14,17-eicosapentaenoic acid

smiles: CCC=CCC=CCC=CCC=CCC=CCCCC(=O)O

enzyme: arachidonate 5-lipoxygenase

substrate: 5,8,11,14,17-eicosapentaenoic acid


substrate_name: 8,11,14-eicosatrienoic acid

smiles: CCCCCC=CCC=CCC=CCCCCCCC(=O)O

substrate: 8,11,14-eicosatrienoic acid


substrate_name: arachidonate

smiles: CCCCCC=CCC=CCC=CCC=CCCCC(=O)[O-]

substrate: arachidonate


substrate_name: 5,8,11-eicosatrienoic acid

smiles: CCCCCCCCC=CCC=CCC=CCCCC(=O)O

substrate: 5,8,11-eicosatrienoic acid


enzyme_display_name: gentisate 1,2-dioxygenase

organism: Aquipseudomonas alcaligenes

assay_conditions_summary: pH 7.4; 296.15 K; buffer 0.1 M phosphate

proof_lines: in 0.1 M phosphate buffer, pH 7.4, at 23°C

enzyme: gentisate 1,2-dioxygenase


substrate_name: gentisate

substrate: gentisate


organism: Pseudomonas putida


organism: Rhodococcus opacus


substrate_name: Unknown

substrate: Unknown


organism: Ruegeria pomeroyi


organism: Xanthobacter polyaromaticivorans

substrate_name: 1-hydroxy 2 naphthoate

substrate: 1-hydroxy 2 naphthoate


enzyme_display_name: homogentisate 1,2-dioxygenase

substrate_name: homogentisate

enzyme: homogentisate 1,2-dioxygenase

substrate: homogentisate


enzyme_display_name: indoleamine 2,3-dioxygenase

organism: Monodelphis domestica

substrate_name: L-tryptophan

enzyme: indoleamine 2,3-dioxygenase

substrate: L-tryptophan


organism: Mus musculus

substrate_name: kynurenine

substrate: kynurenine


organism: Ornithorhynchus anatinus


enzyme_display_name: proline 3-hydroxylase

organism: Streptomyces sp.

substrate_name: L-proline

proof_lines: 100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C

enzyme: proline 3-hydroxylase

substrate: L-proline


substrate_name: DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN

substrate: DESGLPQLTSYDAEVNAPIQGSRNLLQGEELLRALDQVN


proof_lines: pH 7.8, 37°C, Km-value is determined using soluble extracts of cells expressing enzyme-FLAGHis


proof_lines: pH 7.8, 37°C, Ki-value is determined using soluble extracts of cells expressing enzyme-FLAGHis


proof_lines: pH 7.8, 37°C. The Km of FIH for O2 is about 40% of its atmospheric concentration, Km-value is determined using soluble extracts of cells expressing enzyme-FLAGHis


enzyme_display_name: thebaine 6-O-demethylase

organism: Papaver somniferum

enzyme: thebaine 6-O-demethylase


substrate_name: thebaine

substrate: thebaine


substrate_name: oripavine

substrate: oripavine


enzyme_display_name: L-isoleucine 4-hydroxylase

organism: Bacillus thuringiensis

substrate_name: L-leucine

smiles: CC(C)CC(C(=O)O)N

enzyme: L-isoleucine 4-hydroxylase

substrate: L-leucine


substrate_name: L-methionine

smiles: CSCCC(C(=O)O)N

substrate: L-methionine


proof_lines:
at pH 6.0 and 40°C
at pH 6.0 and 25°C


substrate_name: L-isoleucine

smiles: CCC(C)C(C(=O)O)N

proof_lines:
at pH 6.0 and 40°C
at pH 7.0 and 34°C
at pH 7.0 and 35°C

substrate: L-isoleucine


enzyme_display_name: mRNA N(6)-methyladenine demethylase

substrate_name: N3-methylthymine in single-stranded DNA

enzyme: mRNA N(6)-methyladenine demethylase

substrate: N3-methylthymine in single-stranded DNA


enzyme_display_name: ectoine hydroxylase

organism: Paenibacillus lautus

substrate_name: ectoine

enzyme: ectoine hydroxylase

substrate: ectoine


enzyme_display_name: benzoate 1,2-dioxygenase

organism: Acinetobacter calcoaceticus

enzyme: benzoate 1,2-dioxygenase


substrate_name: benzoate

substrate: benzoate


enzyme_display_name: trimethylamine monooxygenase

organism: Methylocella silvestris

substrate_name: N,N,N-trimethylamine

smiles: CN(C)C

assay_conditions_summary: pH 7.6; 295.15 K; buffer 80 mM PIPES

proof_lines: recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C

enzyme: trimethylamine monooxygenase

substrate: N,N,N-trimethylamine


substrate_name: Dimethylsulfide

smiles: CSC

substrate: Dimethylsulfide


substrate_name: Methimazole

substrate: Methimazole


substrate_name: dimethylaniline

substrate: dimethylaniline


substrate_name: N,N-dimethylamine

smiles: CNC

substrate: N,N-dimethylamine


substrate_name: cysteamine

smiles: C(CS)N

substrate: cysteamine


substrate_name: DMSO

smiles: CS(=O)C

substrate: DMSO


organism: Roseovarius sp.


enzyme_display_name: nitric-oxide synthase (NADPH)

organism: Staphylococcus aureus

substrate_name: L-arginine

smiles: C(CC(C(=O)O)N)CN=C(N)N

enzyme: nitric-oxide synthase (NADPH)

substrate: L-arginine


enzyme_display_name: anthraniloyl-CoA monooxygenase

organism: Pseudomonas sp.

assay_conditions_summary: cofactor CoA

proof_lines:
value below, cell extract
value below

enzyme: anthraniloyl-CoA monooxygenase


substrate_name: N-ethylmaleimide

substrate: N-ethylmaleimide


substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

substrate: NADPH


substrate_name: NADH

assay_conditions_summary: cofactor NADH

substrate: NADH


enzyme_display_name: ent-kaurene monooxygenase

organism: Fusarium fujikuroi

enzyme: ent-kaurene monooxygenase


substrate_name: Unknown

substrate: Unknown


enzyme_display_name: isoflavone 3'-hydroxylase

organism: Medicago truncatula

substrate_name: formononetin

assay_conditions_summary: pH 8; cofactor NADPH

enzyme: isoflavone 3'-hydroxylase

substrate: formononetin


substrate_name: Biochanin A

substrate: Biochanin A


enzyme_display_name: trans-cinnamate 4-monooxygenase

organism: Marchantia paleacea

substrate_name: trans-cinnamate

enzyme: trans-cinnamate 4-monooxygenase

substrate: trans-cinnamate


organism: Plagiochasma appendiculatum


organism: Ruta graveolens


enzyme_display_name: leukotriene-B4 20-monooxygenase

organism: Homo sapiens

substrate_name: leukotriene B4

smiles: CCCCCC=CCC(C=CC=CC=CC(CCCC(=O)O)O)O

enzyme: leukotriene-B4 20-monooxygenase

substrate: leukotriene B4


substrate_name: 5-Deoxy-leukotriene B4

substrate: 5-Deoxy-leukotriene B4


proof_lines: recombinant protein CYP4F3, expressed in yeast

proof_lines: recombinant isoform CYP4F3A, expressed in COS-7 cells


substrate_name: Z9(10)-epoxyoctadecanoic acid

proof_lines:
37°C, CYP4F3A
37°C, CYP4F2
37°C, CYP4F3B

substrate: Z9(10)-epoxyoctadecanoic acid


organism: Rattus norvegicus

substrate_name: prostaglandin A1

substrate: prostaglandin A1


proof_lines:
cosubstrate NADPH
cosubstrate NADH
substrate prostaglandin A1


enzyme_display_name: (S)-stylopine synthase

organism: Eschscholzia californica

substrate_name: (S)-scoulerine

proof_lines:
CYP719A3 variant, determined with HPLC
CYP719A2 variant, determined with HPLC

enzyme: (S)-stylopine synthase

substrate: (S)-scoulerine


enzyme_display_name: deacetoxycephalosporin-C synthase

organism: Streptomyces clavuligerus

substrate_name: ampicillin

proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutants R258H and R258A

enzyme: deacetoxycephalosporin-C synthase

substrate: ampicillin


substrate_name: penicillin G

proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258K

proof_lines: pH 7.5, cosubstrate 2-oxoglutarate, recombinant mutant R258K

proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258K

substrate: penicillin G


proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258H

proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258H


proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258A

proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258A


proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258L

proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258L


proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258Q

proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258Q


proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutants R258A and R258F


proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutants R258L and R258F


proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutants R258A and R258H


proof_lines: pH 7.5, cosubstrate 2-oxoglutarate, recombinant wild-type enzyme


proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutants R258H


proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutants R258K, R258A, and R258L


proof_lines: pH 6.5, 30°C, recombinant wild-type enzyme


proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258F

proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258F


proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutants R258A and R258Q


proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutants R258H and R258Q


substrate_name: Penicillin N

mutation_type: deletion

proof_lines: mutant enzyme DELTAI305-310, DELTAR306-310 and mutant DELTAR307-310

substrate: Penicillin N


proof_lines: recombinant mutant T213V, pH not specified in the publication, 30°C


proof_lines: recombinant mutant S261A, pH not specified in the publication, 30°C


proof_lines: recombinant mutant Y184A, pH not specified in the publication, 30°C


proof_lines: recombinant mutant Q126M, pH not specified in the publication, 30°C


proof_lines: recombinant mutant S261M, pH not specified in the publication, 30°C


proof_lines: mutant enzyme C155Y/Y184H/V275I/C281Y/L305M, at pH 7.4 and 25°C


proof_lines: mutant enzyme C155Y/Y184H/V275I/C281Y/I305M/T213V/M73T, at pH 7.4 and 25°C


proof_lines: mutant enzyme C155Y/Y184H, at pH 7.4 and 25°C


proof_lines: mutant I305M/S261M, pH and temperature not specified in the publication


proof_lines: mutantI305M/T213V/M73T, pH and temperature not specified in the publication


proof_lines: mutant I305M/T213V, pH and temperature not specified in the publication


proof_lines: mutant I305M/T213V/S261M, pH and temperature not specified in the publication


proof_lines:
recombinant enzyme
native enzyme


substrate_name: deacetoxycephalosporin C

substrate: deacetoxycephalosporin C


substrate_name: 3-exomethylenecephalosporin C

substrate: 3-exomethylenecephalosporin C


substrate_name: adipyl 6-aminopenicillanic acid

substrate: adipyl 6-aminopenicillanic acid


enzyme_display_name: deoxyhypusine monooxygenase

organism: Cricetulus griseus

enzyme: deoxyhypusine monooxygenase


enzyme_display_name: xanthine oxidase

substrate_name: xanthine

proof_lines: pH 8.5, 25°C, recombinant wild-type enzyme

proof_lines: pH 8.1, 37°C, average value, wild-type enzyme

enzyme: xanthine oxidase

substrate: xanthine


substrate_name: allopurinol

substrate: allopurinol


substrate_name: formycin B

substrate: formycin B


substrate_name: benzaldehyde

substrate: benzaldehyde


substrate_name: N-methylnicotinamide

substrate: N-methylnicotinamide


proof_lines:
heparin-Sepharose 6B-bound enzyme
membrane-bound enzyme
free enzyme


substrate_name: hypoxanthine

substrate: hypoxanthine


enzyme_display_name: ribonucleoside-diphosphate reductase

organism: Corynebacterium nephridii

substrate_name: 2'-methyladenosine 5'-diphosphate

assay_conditions_summary: cofactor ADP

proof_lines:
vs. ADP
vs. GDP

enzyme: ribonucleoside-diphosphate reductase

substrate: 2'-methyladenosine 5'-diphosphate


substrate_name: 2'-methyluridine 5'-diphosphate

proof_lines:
vs. UDP
vs. CDP

substrate: 2'-methyluridine 5'-diphosphate


organism: Falseniella ignava

substrate_name: CDP

proof_lines: mutant NrbBDELTA169, pH and temperature not specified in the publication

substrate: CDP


proof_lines: wild-type NrdB, pH and temperature not specified in the publication


organism: Ovis aries

substrate_name: vitamin K1 2,3-epoxide

substrate: vitamin K1 2,3-epoxide


organism: Clostridium beijerinckii

substrate_name: NADH

proof_lines:
forward reaction, with butyryl-CoA
forward reaction, with acetyl-CoA

substrate: NADH


substrate_name: acetyl-CoA

proof_lines:
forward reaction, with NADPH
forward reaction, with NADH

substrate: acetyl-CoA


substrate_name: CoA

assay_conditions_summary: cofactor NAD+

proof_lines: reverse reaction, with 0.32 mM NAD+ and 11 mM butyraldehyde

substrate: CoA


substrate_name: NADPH

substrate: NADPH


substrate_name: n-butyryl-CoA

substrate: n-butyryl-CoA


substrate_name: NAD+

proof_lines: reverse reaction, with 0.12 mM CoA and 11 mM butyraldehyde

substrate: NAD+


substrate_name: Butyraldehyde

smiles: CCCC=O

proof_lines: reverse reaction, with 0.32 mM NAD+ and 0.12 mM CoA

substrate: Butyraldehyde


enzyme_display_name: aspartate-semialdehyde dehydrogenase

organism: Bacteria

substrate_name: Unknown

enzyme: aspartate-semialdehyde dehydrogenase

substrate: Unknown


organism: Methanocaldococcus jannaschii

substrate_name: NADP+

substrate: NADP+


organism: Neisseria meningitidis

substrate_name: acetaldehyde

smiles: CC=O

assay_conditions_summary: pH 8.5; 298.15 K; buffer 50 mM Tricine

proof_lines: recombinant enzyme, in 50 mM Tricine buffer (pH 8.5), at 25°C

substrate: acetaldehyde


substrate_name: glyceraldehyde

smiles: C(C(C=O)O)O

substrate: glyceraldehyde


substrate_name: D-glyceraldehyde 3-phosphate

smiles: C(C(C=O)O)OP(=O)(O)O

substrate: D-glyceraldehyde 3-phosphate


assay_conditions_summary: pH 8.5; 298.15 K; buffer 50 mM Tricine; cofactor NAD+

proof_lines: recombinant enzyme, in the presence of D-glyceraldehyde 3-phosphate, in 50 mM Tricine buffer (pH 8.5), at 25°C

proof_lines: recombinant enzyme, in 50 mM Tricine buffer (pH 8.5), in the presence of glyceraldehyde, at 25°C

proof_lines: recombinant enzyme, in 50 mM Tricine buffer (pH 8.5), in the presence of acetaldehyde, at 25°C


organism: Spinacia oleracea


organism: Tetrahymena pyriformis

assay_conditions_summary: pH 8.5; 308.15 K; buffer 50 mM Tricine; cofactor NAD+

proof_lines: in 50 mM Tricine buffer, pH 8.5, 10 mM sodium arsenate, at 35°C


assay_conditions_summary: pH 8.5; 308.15 K; buffer 50 mM Tricine


organism: Trichoderma koningii

proof_lines:
GAPDH I
GAPDH II


proof_lines:
GAPDH II
GAPDH I


organism: Streptococcus pyogenes

assay_conditions_summary: pH 7; 303.15 K; cofactor NAD+

proof_lines: recombinant His6-tagged enzyme, pH 7.0, 30°C


assay_conditions_summary: pH 7; 303.15 K; cofactor NADP+


enzyme_display_name: 2,5-dioxovalerate dehydrogenase

organism: Acinetobacter baylyi

substrate_name: succinate semialdehyde

smiles: C(CC(=O)O)C=O

proof_lines:
with NADP+ as cofactor
with NAD+ as cofactor

enzyme: 2,5-dioxovalerate dehydrogenase

substrate: succinate semialdehyde


substrate_name: propanal

smiles: CCC=O

substrate: propanal


substrate_name: Glutaraldehyde

smiles: C(CC=O)CC=O

substrate: Glutaraldehyde


enzyme_display_name: aldehyde dehydrogenase (NAD(+))

organism: Lachnoclostridium phytofermentans

enzyme: aldehyde dehydrogenase (NAD(+))


substrate_name: propionaldehyde

substrate: propionaldehyde


substrate_name: pentanaldehyde

smiles: CCCCC=O

substrate: pentanaldehyde


substrate_name: hexanaldehyde

smiles: CCCCCC=O

substrate: hexanaldehyde


enzyme_display_name: aldehyde dehydrogenase (NADP(+))

organism: Paenarthrobacter nicotinovorans

substrate_name: formaldehyde

smiles: C=O

assay_conditions_summary: pH 8.4; 301.15 K; cofactor NADP+

enzyme: aldehyde dehydrogenase (NADP(+))

substrate: formaldehyde


assay_conditions_summary: pH 8.4; 301.15 K; cofactor NAD+


substrate_name: benzaldehyde

substrate: benzaldehyde


organism: Saccharomyces cerevisiae

assay_conditions_summary: cofactor NADP+


substrate_name: glycolaldehyde

smiles: C(C=O)O

substrate: glycolaldehyde


enzyme_display_name: cinnamoyl-CoA reductase

organism: Selaginella moellendorffii

proof_lines: mutant S123T of isoform CCR1, pH and temperature not specified in the publication

enzyme: cinnamoyl-CoA reductase


substrate_name: sinapoyl-CoA

substrate: sinapoyl-CoA


substrate_name: caffeoyl-CoA

substrate: caffeoyl-CoA


substrate_name: feruloyl-CoA

substrate: feruloyl-CoA


proof_lines: isoform CCR2-1, pH and temperature not specified in the publication

proof_lines: isoform CCR1, pH and temperature not specified in the publication


organism: Vitis vinifera

assay_conditions_summary: pH 6; cofactor CoA

proof_lines: at pH 6.0, temperature not specified in the publication


enzyme_display_name: formaldehyde dehydrogenase

organism: Escherichia coli

proof_lines: pH and temperature not specified in the publication

enzyme: formaldehyde dehydrogenase


enzyme_display_name: long-chain-aldehyde dehydrogenase

organism: Homo sapiens

substrate_name: Tetradecanal

smiles: CCCCCCCCCCCCCC=O

enzyme: long-chain-aldehyde dehydrogenase

substrate: Tetradecanal


substrate_name: farnesal

smiles: CC(=CCCC(=CCCC(=CC=O)C)C)C

substrate: farnesal


substrate_name: hexadecanal

smiles: CCCCCCCCCCCCCCCC=O

substrate: hexadecanal


substrate_name: NAD+

substrate: NAD+


smiles: CCCCC=CCCCCCCCCCC=O


substrate_name: octadecanal

smiles: CCCCCCCCCCCCCCCCCC=O

substrate: octadecanal


substrate_name: decanal

smiles: CCCCCCCCCC=O

substrate: decanal


substrate_name: dodecanal

smiles: CCCCCCCCCCCC=O

substrate: dodecanal


smiles: CCCCCCCCC=CCCCCCCCC=O


smiles: CCCCCCC=CCCCCCCCC=O


substrate_name: Pentadecanal

smiles: CCCCCCCCCCCCCCC=O

substrate: Pentadecanal


substrate_name: octanal

smiles: CCCCCCCC=O

substrate: octanal


substrate_name: hexanal

substrate: hexanal


substrate_name: crotonaldehyde

smiles: CC=CC=O

substrate: crotonaldehyde


substrate_name: propionaldehyde

smiles: CCC=O

substrate: propionaldehyde


substrate_name: benzaldehyde

substrate: benzaldehyde


substrate_name: Glutaraldehyde

smiles: C(CC=O)CC=O

substrate: Glutaraldehyde


substrate_name: acetaldehyde

smiles: CC=O

substrate: acetaldehyde


substrate_name: NADP+

substrate: NADP+


enzyme_display_name: aldehyde dehydrogenase [NAD(P)(+)]

organism: Cupriavidus necator

enzyme: aldehyde dehydrogenase [NAD(P)(+)]


proof_lines:
reaction with NADP+, enzyme form ALDH2
reaction with NADP+, enzyme form ALDH1
reaction with NADP+, enzyme form ALDH5


substrate_name: propanal

proof_lines:
reaction with NAD+, enzyme form ALDH2
reaction with NADP+, enzyme form ALDH2
reaction with NADP+, enzyme form ALDH1

substrate: propanal


proof_lines: reaction with acetaldehyde, enzyme form ALDH1

proof_lines: reaction with propanal, enzyme form ALDH1

proof_lines: reaction with propanal, enzyme form ALDH2


proof_lines: reaction with propanal, enzyme form ALDH5


organism: Klebsiella pneumoniae


substrate_name: succinate semialdehyde

smiles: C(CC(=O)O)C=O

substrate: succinate semialdehyde


enzyme_display_name: betaine-aldehyde dehydrogenase

organism: Amaranthus hypochondriacus

substrate_name: Betaine aldehyde

enzyme: betaine-aldehyde dehydrogenase

substrate: Betaine aldehyde


substrate_name: Unknown

proof_lines:
with respect to NAD+
with respect to betaine aldehyde

substrate: Unknown


smiles: C[S+](C)CCC=O


substrate_name: NAD+

substrate: NAD+


smiles: C(CC=O)CN


proof_lines:
wild type enzyme, at pH 8.0 and 30°C
wild-type enzyme


assay_conditions_summary: pH 8; 303.15 K; cofactor NAD+

proof_lines: wild-type enzyme, reaction with betaine aldehyde

proof_lines: wild-type enzyme, reaction with 4-aminopropionaldehyde


assay_conditions_summary: pH 9; cofactor NADP+

proof_lines: pH 9.0, temperature not specified in the publication


substrate_name: CoA

assay_conditions_summary: pH 9; cofactor CoA

substrate: CoA


assay_conditions_summary: pH 9


organism: Saccharolobus solfataricus

substrate_name: D-glyceraldehyde 3-phosphate

smiles: C(C(C=O)O)OP(=O)(O)O

proof_lines: in the presence of D-glucose 1-phosphate

substrate: D-glyceraldehyde 3-phosphate


assay_conditions_summary: pH 7; 343.15 K; cofactor NADP+

proof_lines: in 90 mM HEPES/KOH, 160 mM KCl, pH 7.0, at 70°C

proof_lines: in the absence or presence of D-glucose 1-phosphate


assay_conditions_summary: pH 7; 343.15 K; cofactor NAD+


organism: Thermoproteus tenax


assay_conditions_summary: pH 7; 318.15 K; cofactor NAD+

proof_lines:
pH 7.0, 45°C
pH 7.0, 70°C
pH 7.0, 70°C, recombinant enzyme


enzyme_display_name: aldehyde oxidase

organism: Arabidopsis thaliana

proof_lines: partially purified protein, pH 7.0, 30°C

enzyme: aldehyde oxidase


substrate_name: cinnamylaldehyde

substrate: cinnamylaldehyde


organism: Pseudomonas sp.

substrate_name: hexylaldehyde

smiles: CCCCCC=O

substrate: hexylaldehyde


substrate_name: Butyraldehyde

smiles: CCCC=O

substrate: Butyraldehyde


substrate_name: p-Methoxybenzaldehyde

substrate: p-Methoxybenzaldehyde


substrate_name: Valeraldehyde

smiles: CCCCC=O

substrate: Valeraldehyde


substrate_name: p-Hydroxybenzaldehyde

substrate: p-Hydroxybenzaldehyde


substrate_name: propionaldehyde

substrate: propionaldehyde


substrate_name: Glyoxal

smiles: C(=O)C=O

substrate: Glyoxal


substrate_name: n-Heptylaldehyde

smiles: CCCCCCC=O

substrate: n-Heptylaldehyde


substrate_name: Glutaraldehyde

substrate: Glutaraldehyde


substrate_name: DL-glyceraldehyde

smiles: C(C(C=O)O)O

substrate: DL-glyceraldehyde


substrate_name: Vanillin

substrate: Vanillin


substrate_name: formaldehyde

smiles: C=O

substrate: formaldehyde


smiles: C(CO)C=O

proof_lines: recombinant cell extract, expression in Klebsiella pneumoniae, pH 7.0, 37°C


substrate_name: Salicylaldehyde

substrate: Salicylaldehyde


substrate_name: acrolein

smiles: C=CC=O

substrate: acrolein


substrate_name: citral

smiles: CC(=CCCC(=CC=O)C)C

substrate: citral


enzyme_display_name: pyruvate synthase

organism: Thermoanaerobacter kivui

substrate_name: oxidized ferredoxin

enzyme: pyruvate synthase

substrate: oxidized ferredoxin


substrate_name: CoA

assay_conditions_summary: pH 7.5; 339.15 K; cofactor CoA

substrate: CoA


substrate_name: pyruvate

substrate: pyruvate


organism: Thermococcus kodakarensis


assay_conditions_summary: pH 9.5; 358.15 K; cofactor CoA


enzyme_display_name: 2-oxoacid oxidoreductase (ferredoxin)

organism: Sulfolobus sp.

proof_lines:
pH 7.5, 80°C, recombinant enzyme
pH 7.5, 80°C, natural enzyme
pH 6.8, 50°C

enzyme: 2-oxoacid oxidoreductase (ferredoxin)


proof_lines:
pH 7.5, 80°C, recombinant enzyme
pH 7.5, 80°C, naturale enzyme
pH 6.8, 50°C


proof_lines: pH 7.5, 80°C, natural and recombinant wild-type enzyme


smiles: CCC(=O)C(=O)O

proof_lines: pH 7.5, 80°C, recombinant wild-type enzyme


enzyme_display_name: 2-oxoglutarate synthase

organism: Hydrogenobacter thermophilus

assay_conditions_summary: pH 7.8; 343.15 K; cofactor CoA

proof_lines:
pH 7.8, 70°C, recombinant For
pH 7.6, 70°C
pH 7.8, 70°C, recombinant Kor

enzyme: 2-oxoglutarate synthase


proof_lines:
pH 7.8, 70°C, recombinant Kor
pH 7.6, 70°C
pH 7.8, 70°C, recombinant For


organism: Thauera aromatica


assay_conditions_summary: pH 7.8; 303.15 K; cofactor CoA


enzyme_display_name: prephenate dehydrogenase

organism: Pseudomonas flexibilis

substrate_name: Unknown

assay_conditions_summary: cofactor NAD+

proof_lines:
with prephenate as variable substrate
with NAD+ as variable substrate

enzyme: prephenate dehydrogenase

substrate: Unknown


enzyme_display_name: prephenate dehydrogenase (NADP(+))

organism: Glycine max

substrate_name: prephenate

proof_lines: at 37°C, pH not specified in the publication

enzyme: prephenate dehydrogenase (NADP(+))

substrate: prephenate


enzyme_display_name: dihydroorotate dehydrogenase (NAD(+))

organism: Faecalicatena orotica

enzyme: dihydroorotate dehydrogenase (NAD(+))


substrate_name: Orotate

assay_conditions_summary: pH 8; cofactor NAD+

proof_lines: formation of L-dihydroorotate, in absence of NAD+ or NADH, pH 8, 10 mM cysteine

substrate: Orotate


substrate_name: L-dihydroorotate

assay_conditions_summary: pH 8; 298.15 K; cofactor NAD+

proof_lines: formation of orotate, in absence of NAD+ or NADH, pH 8, 10 mM cysteine

substrate: L-dihydroorotate


substrate_name: NAD+

substrate: NAD+


organism: Macaca fuscata

substrate_name: dihydroxyacetone phosphate

smiles: C(C(=O)COP(=O)(O)O)O

substrate: dihydroxyacetone phosphate


substrate_name: dihydroxyacetone

smiles: C(C(=O)CO)O

substrate: dihydroxyacetone


substrate_name: L-glyceraldehyde

substrate: L-glyceraldehyde


substrate_name: trans-benzene dihydrodiol

substrate: trans-benzene dihydrodiol


substrate_name: Camphorquinone

assay_conditions_summary: cofactor NADPH

substrate: Camphorquinone


substrate_name: Naphthalene dihydrodiol

substrate: Naphthalene dihydrodiol


substrate_name: NADP+

assay_conditions_summary: buffer 0; cofactor NADP+

substrate: NADP+


substrate_name: NADPH

substrate: NADPH


substrate_name: D-xylose

assay_conditions_summary: buffer 25 mM'; cofactor NADP+

proof_lines: mutant H79Q, NADP+, 0.1 M phosphate buffer

substrate: D-xylose


proof_lines:
phosphate buffer 0.1 M
Tris buffer 25 mM


substrate_name: diacetyl

smiles: CC(=O)C(=O)C

substrate: diacetyl


smiles: CCC(=O)C(=O)C


assay_conditions_summary: buffer 25 mM; cofactor NADP+

proof_lines:
NADP+, 25 mM Tris buffer 25 mM
NADP+, 0.1 M phosphate buffer


proof_lines: wild type enzyme, in 25 mM Tris-HCl pH 7.5, at 25°C


organism: Macaca fuscata fuscata

substrate_name: (S)-tetralol

substrate: (S)-tetralol


organism: Mus musculus

substrate_name: cis-benzene dihydrodiol

assay_conditions_summary: cofactor NADP+

proof_lines:
NAD+, DD1
NAD+
NADP+, DD3

substrate: cis-benzene dihydrodiol


enzyme_display_name: maleylacetate reductase

substrate_name: maleylacetate

enzyme: maleylacetate reductase

substrate: maleylacetate


substrate_name: NADH

assay_conditions_summary: cofactor NADH

substrate: NADH


smiles: CC(C(=O)C=CC(=O)O)C(=O)O


smiles: CC(C(=O)C=C(C(=O)O)Cl)C(=O)O


enzyme_display_name: protochlorophyllide reductase

organism: Lathyrus oleraceus

substrate_name: protochlorophyllide

enzyme: protochlorophyllide reductase

substrate: protochlorophyllide


substrate_name: protochlorophyllide a

proof_lines: fusion protein with maltose-binding protein

substrate: protochlorophyllide a


organism: Pinus mugo

proof_lines: pH and temperature not specified in the publication


enzyme_display_name: trans-2-enoyl-CoA reductase (NADPH)

organism: Homo sapiens

substrate_name: trans-phytenoyl-CoA

assay_conditions_summary: pH 7.2; 310.15 K; cofactor CoA

enzyme: trans-2-enoyl-CoA reductase (NADPH)

substrate: trans-phytenoyl-CoA


assay_conditions_summary: pH 7.2; 310.15 K; cofactor NADPH


enzyme_display_name: 12-oxophytodienoate reductase

organism: Oryza sativa

enzyme: 12-oxophytodienoate reductase


enzyme_display_name: arogenate dehydrogenase

organism: Zymomonas mobilis

substrate_name: NAD+

enzyme: arogenate dehydrogenase

substrate: NAD+


substrate_name: L-arogenate

substrate: L-arogenate


substrate_name: prephenate

substrate: prephenate


enzyme_display_name: trans-2-enoyl-CoA reductase (NAD(+))

organism: Clostridium acetobutylicum

assay_conditions_summary: pH 6.2; 298.15 K; cofactor NADH

proof_lines:
pH 6.2, 25°C, mutant Y225A
mutant enzyme Y225A, at pH 6.2 and 25°C

enzyme: trans-2-enoyl-CoA reductase (NAD(+))


proof_lines:
pH 6.2, 25°C, mutant F11K
mutant enzyme F11K, at pH 6.2 and 25°C


proof_lines:
pH 6.2, 25°C, wild-type
wild type enzyme, at pH 6.2 and 25°C


organism: Comamonas testosteroni

substrate_name: NAD(P)+

assay_conditions_summary: pH 9; 298.15 K; cofactor NADP+

substrate: NAD(P)+


assay_conditions_summary: pH 9.5; 310.15 K; cofactor NAD+

proof_lines: activity measured with 1 micromol NAD+ in the reaction mixture at pH 9.5 and 37°C


assay_conditions_summary: pH 9; 298.15 K; cofactor NAD+

proof_lines:
with 2.5 mM NAD+
at pH 9 and 25°C


enzyme_display_name: 2-alkenal reductase [NAD(P)(+)]

substrate_name: (-)-hydroxymethylacylfulvene

enzyme: 2-alkenal reductase [NAD(P)(+)]

substrate: (-)-hydroxymethylacylfulvene


substrate_name: (+)-acylfulvene

substrate: (+)-acylfulvene


organism: Rattus norvegicus

substrate_name: acrolein

substrate: acrolein


substrate_name: 15-oxoprostaglandin E1

substrate: 15-oxoprostaglandin E1


smiles: CCCCCC(C=CC=O)O


substrate_name: ethyl vinyl ketone

smiles: CCC(=O)C=C

substrate: ethyl vinyl ketone


substrate_name: illudin M

substrate: illudin M


substrate_name: irofulven

substrate: irofulven


substrate_name: illudin S

substrate: illudin S


substrate_name: 15-deoxy-DELTA12,14-prostaglandin J2

substrate: 15-deoxy-DELTA12,14-prostaglandin J2


smiles: CCC=CC=O


substrate_name: NADPH

assay_conditions_summary: pH 7.2; 298.15 K; cofactor NADPH

proof_lines: recombinant enzyme, pH 7.2, 25°C, with substrate trans-2-nonenal

proof_lines: recombinant enzyme, pH 7.2, 25°C, with substrate trans-3-nonen-2-one

substrate: NADPH


smiles: CCCCCC=CC(=O)C


smiles: CCCCCCC=CC=O


enzyme_display_name: 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH)

organism: Chlorobaculum tepidum

substrate_name: 3,8-divinyl chlorophyllide a

proof_lines: recombinant BciA, pH and temperature not specified in the publication

substrate: 3,8-divinyl chlorophyllide a


enzyme_display_name: arogenate dehydrogenase (NADP(+))

organism: Arabidopsis thaliana

enzyme: arogenate dehydrogenase (NADP(+))


substrate_name: NADP+

proof_lines:
recombinant from plasmid TyrA-AT2
recombinant from plasmid TyrA-ATcM36
recombinant from plasmid TyrA-ATlM36

substrate: NADP+


proof_lines: recombinant from plasmids TyrA-AT2 and TyrA-ATlM36


substrate_name: Unknown

proof_lines:
recombinant from plasmid TyrA-ATlM36
recombinant from plasmid TyrA-AT2
recombinant from plasmid TyrA-ATcM36

substrate: Unknown


organism: Candidatus Liberibacter asiaticus

substrate_name: crotonyl-CoA

assay_conditions_summary: pH 7.5; 301.15 K; cofactor CoA

substrate: crotonyl-CoA


substrate_name: NADH

assay_conditions_summary: pH 7.5; 301.15 K; cofactor NADH

substrate: NADH


proof_lines: with respect to NADH, at pH 7.5 and 28°C

proof_lines: with respect to crotonyl-CoA, at pH 7.5 and 28°C


organism: Plasmodium falciparum

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADP+

proof_lines: substrate NADH, pH not specified in the publication, temperature not specified in the publication


assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADH


assay_conditions_summary: pH 7.5; 298.15 K; cofactor CoA


assay_conditions_summary: cofactor NAD+

proof_lines: wild-type, substrate NADH, pH not specified in the publication, temperature not specified in the publication

proof_lines: wild-type, substrate NAD+, pH not specified in the publication, temperature not specified in the publication


enzyme_display_name: acryloyl-CoA reductase (NADH)

organism: Anaerotignum propionicum

assay_conditions_summary: pH 7; 298.15 K; cofactor NADH

proof_lines: using acryloyl-CoA as cosubstrate, in 50 mM Tris/HCl, pH 7.0, at 25°C

enzyme: acryloyl-CoA reductase (NADH)


substrate_name: acryloyl-CoA

assay_conditions_summary: pH 7; 298.15 K; cofactor CoA

proof_lines: apparent value, in 50 mM Tris/HCl, pH 7.0, at 25°C

substrate: acryloyl-CoA


smiles: CC(=O)C=C


enzyme_display_name: botryococcene synthase

organism: Botryococcus braunii

enzyme: botryococcene synthase


enzyme_display_name: coproporphyrinogen oxidase

substrate_name: Coproporphyrinogen-III

proof_lines:
mutant H158A
mutant enzyme H158A, at 37°C

enzyme: coproporphyrinogen oxidase

substrate: Coproporphyrinogen-III


substrate_name: Harderoporphyrinogen

substrate: Harderoporphyrinogen


proof_lines:
wild-type
wild type enzyme, at 37°C


proof_lines: wild-type, divinyl product, 37°C, pH not specified in the publication

proof_lines: wild-type, total product, 37°C, pH not specified in the publication


proof_lines:
with monovinyl product
monovinyl as product
divinyl as product


proof_lines:
at 37°C
monovinyl as product
total product


substrate_name: mesoporphyrinogen-VI

proof_lines: wild-type, monovinyl product, 37°C, pH not specified in the publication

substrate: mesoporphyrinogen-VI


organism: Nicotiana tabacum


enzyme_display_name: protoporphyrinogen oxidase

organism: Hordeum vulgare

substrate_name: protoporphyrinogen-IX

enzyme: protoporphyrinogen oxidase

substrate: protoporphyrinogen-IX


enzyme_display_name: acyl-CoA oxidase

organism: Cucumis sativus

substrate_name: palmitoyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: acyl-CoA oxidase

substrate: palmitoyl-CoA


organism: Paenarthrobacter ureafaciens


enzyme_display_name: succinate dehydrogenase

organism: Wolinella succinogenes

substrate_name: succinate

assay_conditions_summary: cofactor FAD

proof_lines: with 2,3-dimethyl-1,4-naphthoquinone, based on FAD content of enzyme

enzyme: succinate dehydrogenase

substrate: succinate


substrate_name: fumarate

proof_lines: based on the content of the MW 79000 peptide, reduction of fumarate with dimethylnaphthohydroquinone

proof_lines: with 2,3-dimethyl-1,4-naphthohydroquinone, based on FAD content of enzyme

substrate: fumarate


enzyme_display_name: dihydroorotate dehydrogenase (quinone)

organism: Homo sapiens

substrate_name: dihydroorotate

proof_lines:
pH 8.2, 25°C
cosubstrate ubiquinone-50
cosubstrate decylubiquinone

enzyme: dihydroorotate dehydrogenase (quinone)

substrate: dihydroorotate


substrate_name: coenzyme Q0

substrate: coenzyme Q0


substrate_name: S-dihydroorotate

proof_lines:
expressed in Escherichia coli
cosubstrate dihydroorotate
full-length native enzyme

substrate: S-dihydroorotate


substrate_name: decylubiquinone

proof_lines:
expressed in Escherichia coli
cosubstrate dihydroorotate

substrate: decylubiquinone


organism: Solanum tuberosum

substrate_name: (S)-dihydroorotate

substrate: (S)-dihydroorotate


substrate_name: coenzyme QD

substrate: coenzyme QD


enzyme_display_name: 9,9'-di-cis-zeta-carotene desaturase

organism: Capsicum annuum

substrate_name: zeta-carotene

enzyme: 9,9'-di-cis-zeta-carotene desaturase

substrate: zeta-carotene


substrate_name: Neurosporene

substrate: Neurosporene


enzyme_display_name: short-chain acyl-CoA dehydrogenase

organism: Pseudomonas putida

substrate_name: octanoyl-CoA

enzyme: short-chain acyl-CoA dehydrogenase

substrate: octanoyl-CoA


substrate_name: heptanoyl-CoA

substrate: heptanoyl-CoA


substrate_name: butyryl-CoA

assay_conditions_summary: pH 5.5; cofactor CoA

proof_lines:
at pH 5.5
at pH 6.0
at pH 8.0

substrate: butyryl-CoA


substrate_name: hexanoyl-CoA

substrate: hexanoyl-CoA


substrate_name: valeryl-CoA

substrate: valeryl-CoA


enzyme_display_name: isovaleryl-CoA dehydrogenase

organism: Caenorhabditis elegans

proof_lines: using recombinant enzyme, electron acceptor: electron-transferring flavoprotein

enzyme: isovaleryl-CoA dehydrogenase


substrate_name: isovaleryl-CoA

substrate: isovaleryl-CoA


substrate_name: isobutyryl-CoA

substrate: isobutyryl-CoA


assay_conditions_summary: 305.15 K; cofactor CoA


organism: Ascaris suum


enzyme_display_name: glutaryl-CoA dehydrogenase (ETF)

organism: Paracoccus denitrificans

substrate_name: electron-transfer flavoprotein

enzyme: glutaryl-CoA dehydrogenase (ETF)

substrate: electron-transfer flavoprotein


substrate_name: phenylmethylsulfonyl fluoride

substrate: phenylmethylsulfonyl fluoride


substrate_name: glutaryl-CoA

substrate: glutaryl-CoA


enzyme_display_name: isoquinoline 1-oxidoreductase

organism: Brevundimonas diminuta

substrate_name: Isoquinoline

proof_lines: with 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazolium chloride as cosubstrate

enzyme: isoquinoline 1-oxidoreductase

substrate: Isoquinoline


enzyme_display_name: phytoene desaturase (lycopene-forming)

organism: Pantoea ananatis

proof_lines: recombinant His6-tagged enzyme, pH and temperature not specified in the publication

enzyme: phytoene desaturase (lycopene-forming)


enzyme_display_name: alanine dehydrogenase

organism: Anabaena cylindrica

substrate_name: pyruvate

enzyme: alanine dehydrogenase

substrate: pyruvate


substrate_name: L-Ala

smiles: CC(C(=O)O)N

substrate: L-Ala


substrate_name: oxaloacetate

substrate: oxaloacetate


organism: Helicobacter aurati


substrate_name: L-alanine

substrate: L-alanine


organism: Streptomyces anulatus

substrate_name: NADH

assay_conditions_summary: pH 8.5; 313.15 K; cofactor NADH

substrate: NADH


substrate_name: NAD+

assay_conditions_summary: pH 10; 313.15 K; cofactor NAD+

substrate: NAD+


organism: Vibrio proteolyticus

assay_conditions_summary: 298.15 K; cofactor NADH


smiles: N


assay_conditions_summary: 298.15 K; cofactor NAD+


enzyme_display_name: glutamate synthase (NADPH)

organism: Pyrococcus horikoshii

substrate_name: NADPH

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NADPH

proof_lines:
pH 7.5, 37°C, pGLTY2
pH 7.5, 37°C, pGLTZ
pH 7.5, 70°C, pGLTZ

enzyme: glutamate synthase (NADPH)

substrate: NADPH


organism: Rhodospirillum rubrum

assay_conditions_summary: cofactor NADPH


substrate_name: L-glutamine

smiles: C(CC(=O)N)C(C(=O)O)N

substrate: L-glutamine


enzyme_display_name: glutamate synthase (NADH)

organism: Lupinus angustifolius

substrate_name: 3-acetylpyridine adenine dinucleotide

assay_conditions_summary: cofactor NADH

proof_lines: reduced form as reductant, glutamate synthase reaction

proof_lines: NADH-dependent reduction of oxidized form of 3-acetylpyridine adenine dinucleotide

enzyme: glutamate synthase (NADH)

substrate: 3-acetylpyridine adenine dinucleotide


proof_lines: with reduced form of 3-acetylpyridine adenine dinucleotide as reductant


substrate_name: Unknown

substrate: Unknown


assay_conditions_summary: pH 6.5; cofactor NADH


enzyme_display_name: diaminopimelate dehydrogenase

organism: Acetivibrio thermocellus

enzyme: diaminopimelate dehydrogenase


smiles: C(CC(C(=O)O)N)CC(C(=O)O)N


enzyme_display_name: N-methylalanine dehydrogenase

organism: Pseudomonas sp.

enzyme: N-methylalanine dehydrogenase


substrate_name: methylamine

smiles: CN

substrate: methylamine


enzyme_display_name: glutamate dehydrogenase

organism: Bacillus cereus

proof_lines: from double-reciprocal plots for concentrations between 5 mM and 200 mM

enzyme: glutamate dehydrogenase


substrate_name: glutamate

smiles: C(CC(=O)O)C(C(=O)O)N

substrate: glutamate


organism: Bos taurus

assay_conditions_summary: pH 7.5; cofactor NAD+

proof_lines: 0.008 mM inhibitor 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one, Vmax: 0.04, pH 7.5

proof_lines: 0.0015 mM inhibitor 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one, Vmax: 0.13, pH 7.5

proof_lines: 0.009 mM inhibitor biothionol, Vmax: 0.05, pH 7.5


substrate_name: L-glutamate

proof_lines: 0.005 mM inhibitor biothionol, Vmax: 0.1, pH 7.5

proof_lines: 0.003 mM inhibitor biothionol, Vmax: 0.14, pH 7.5

substrate: L-glutamate


organism: Laccaria bicolor


enzyme_display_name: aspartate dehydrogenase

organism: Pseudomonas aeruginosa

substrate_name: L-aspartate

assay_conditions_summary: pH 9.8; 310.15 K; cofactor NADP+

proof_lines:
pH 9.8, 37°C, with NADP+
pH 9.8, 37°C, with NAD+

enzyme: aspartate dehydrogenase

substrate: L-aspartate


substrate_name: NADP+

substrate: NADP+


assay_conditions_summary: pH 8.2; 310.15 K; cofactor NADPH

proof_lines:
pH 8.2, 37°C, with NADH
pH 8.2, 37°C, with NADPH


assay_conditions_summary: pH 9.8; 310.15 K; cofactor NAD+


assay_conditions_summary: pH 8.2; 310.15 K; cofactor NADH


enzyme_display_name: glutamate dehydrogenase [NAD(P)(+)]

organism: Pyrococcus furiosus

assay_conditions_summary: cofactor NADP+

enzyme: glutamate dehydrogenase [NAD(P)(+)]


assay_conditions_summary: cofactor NAD+


proof_lines:
substrate 2-oxoglutarate
substrate NH3


proof_lines:
cofactor NADP+
cofactor NAD+


proof_lines:
cofactor NADPH
cofactor NADH


enzyme_display_name: glutamate dehydrogenase (NADP(+))

organism: Aspergillus terreus

assay_conditions_summary: 301.15 K; cofactor NADP+

enzyme: glutamate dehydrogenase (NADP(+))


assay_conditions_summary: 301.15 K; cofactor NADPH


organism: Psychrobacter sp.


organism: Trypanosoma cruzi


enzyme_display_name: leucine dehydrogenase

organism: Sporosarcina psychrophila

substrate_name: L-leucine

smiles: CC(C)CC(C(=O)O)N

enzyme: leucine dehydrogenase

substrate: L-leucine


assay_conditions_summary: pH 10.5; 293.15 K; cofactor NAD+


organism: Thermoactinomyces intermedius


substrate_name: L-Ile

smiles: CCC(C)C(C(=O)O)N

substrate: L-Ile


smiles: CC(C)CC(=O)C(=O)O


smiles: CCCCC(=O)C(=O)[O-]


substrate_name: L-Leu

substrate: L-Leu


smiles: CCC(C)C(=O)C(=O)O


substrate_name: L-Val

smiles: CC(C)C(C(=O)O)N

substrate: L-Val


smiles: CCC(=O)C(=O)O


proof_lines: mutant enzyme D203A/I204R and D203A/I204R/D210R


enzyme_display_name: D-aspartate oxidase

organism: Oncorhynchus mykiss

substrate_name: D-Aspartate

enzyme: D-aspartate oxidase

substrate: D-Aspartate


enzyme_display_name: L-amino-acid oxidase

organism: Cerastes cerastes

proof_lines:
at pH 7.8 and 50°C
at pH 8.0 and 20°C
isoform LAAOI, at pH 7.8 and 50°C

enzyme: L-amino-acid oxidase


substrate_name: L-Met

smiles: CSCCC(C(=O)O)N

substrate: L-Met


substrate_name: L-Phe

substrate: L-Phe


organism: Meleagris gallopavo

substrate_name: L-lysine

smiles: C(CCN)CC(C(=O)O)N

substrate: L-lysine


substrate_name: L-tyrosine

substrate: L-tyrosine


substrate_name: L-tryptophan

substrate: L-tryptophan


substrate_name: L-arginine

smiles: C(CC(C(=O)O)N)CN=C(N)N

substrate: L-arginine


substrate_name: L-phenylalanine

substrate: L-phenylalanine


substrate_name: L-histidine

substrate: L-histidine


substrate_name: L-ornithine

smiles: C(CC(C(=O)O)N)CN

substrate: L-ornithine


substrate_name: L-methionine

substrate: L-methionine


organism: Rhodococcus opacus

substrate_name: L-alanine

smiles: CC(C(=O)O)N

proof_lines:
wild-type enzyme
wild-type enzyme, 30°C, pH 7.6

substrate: L-alanine


proof_lines: recombinant enzyme, expressed in and Streptomyces lividans, 30°C, pH 7.6

proof_lines: recombinant enzyme, expressed in Streptomyces lividans, 30°C, pH 7.6


enzyme_display_name: primary-amine oxidase

organism: Lathyrus sativus

smiles: C(CN)C#CCN

enzyme: primary-amine oxidase


enzyme_display_name: diamine oxidase

organism: Homo sapiens

substrate_name: histamine

proof_lines: enzyme from seminal plasma, at 37°C, pH not specified in the publication

proof_lines: enzyme from placenta, at 37°C, pH not specified in the publication

enzyme: diamine oxidase

substrate: histamine


substrate_name: spermine

smiles: C(CCNCCCN)CNCCCN

substrate: spermine


substrate_name: Methylhistamine

proof_lines: 30°C, pH 7.2, with respect to reductive half-reaction

substrate: Methylhistamine


substrate_name: Butylamine

smiles: CCCCN

substrate: Butylamine


substrate_name: ethylenediamine

smiles: C(CN)N

substrate: ethylenediamine


substrate_name: spermidine

smiles: C(CCNCCCN)CN

substrate: spermidine


substrate_name: L-Lysine methyl ester

smiles: COC(=O)C(CCCCN)N

substrate: L-Lysine methyl ester


substrate_name: cadaverine

smiles: C(CCN)CCN

substrate: cadaverine


smiles: C(CN)CN


substrate_name: p-dimethylaminomethylbenzylamine

substrate: p-dimethylaminomethylbenzylamine


substrate_name: putrescine

smiles: C(CCN)CN

proof_lines:
at pH 8.0 and 30°C
30°C, pH 7.2, per dimer
pH 7.2, 37°C

substrate: putrescine


substrate_name: N(tau)-methylhistamine

substrate: N(tau)-methylhistamine


smiles: O=O

proof_lines:
30°C, pH 7.2, per dimer
30°C, pH 7.2


enzyme_display_name: D-amino-acid oxidase

organism: Glutamicibacter protophormiae

substrate_name: Unknown

enzyme: D-amino-acid oxidase

substrate: Unknown


organism: Neurospora crassa

substrate_name: D-methionine

substrate: D-methionine


substrate_name: D-leucine

smiles: CC(C)CC(C(=O)O)N

substrate: D-leucine


enzyme_display_name: monoamine oxidase

organism: Danio rerio

substrate_name: benzylamine

proof_lines: at 25°C in 50 mM potassium phosphate, pH 7.4

enzyme: monoamine oxidase

substrate: benzylamine


proof_lines:
pH 7.4, 25°C, with benzylamine
pH 7.4, 25°C, with phenylethylamine


organism: Gallus gallus

substrate_name: glycine

smiles: C(C(=O)O)N

substrate: glycine


enzyme_display_name: glutamate synthase (ferredoxin)

organism: Spinacia oleracea

enzyme: glutamate synthase (ferredoxin)


substrate_name: L-glutamine

smiles: C(CC(=O)N)C(C(=O)O)N

substrate: L-glutamine


organism: Synechocystis sp.

substrate_name: oxidized ferredoxin

proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication

substrate: oxidized ferredoxin


proof_lines: mutant Q467A, pH not specified in the publication, temperature not specified in the publication


substrate_name: reduced ferredoxin

proof_lines: L-glutamate:iodonitrotetrazolium oxidoreductase activity at pH 9.5

substrate: reduced ferredoxin


proof_lines:
native enzyme
recombinant enzyme


proof_lines:
recombinant enzyme
native enzyme


substrate_name: L-glutamate

smiles: C(CC(=O)O)C(C(=O)O)N

substrate: L-glutamate


organism: Synechocystis sp. PCC 6803

assay_conditions_summary: pH 8

proof_lines: wild type enzyme, at pH 8.0, temperature not specified in the publication


enzyme_display_name: aralkylamine dehydrogenase (azurin)

organism: Alcaligenes faecalis

substrate_name: tyramine

enzyme: aralkylamine dehydrogenase (azurin)

substrate: tyramine


substrate_name: phenazine methosulfate

substrate: phenazine methosulfate


substrate_name: phenazine ethosulfate

substrate: phenazine ethosulfate


substrate_name: dopamine

substrate: dopamine


substrate_name: azurin

assay_conditions_summary: buffer 0.01 M potassium phosphate

proof_lines:
in 0.01 M potassium phosphate buffer
in 0.25 M potassium phosphate buffer

substrate: azurin


proof_lines:
pH 7.5, 25°C, recombinant enzyme
pH 7.5, 25°C, native enzyme


enzyme_display_name: pyrroline-5-carboxylate reductase

organism: Acetoanaerobium sticklandii

substrate_name: L-proline

enzyme: pyrroline-5-carboxylate reductase

substrate: L-proline


substrate_name: NAD+

substrate: NAD+


organism: Chlorella vulgaris var. autotrophica

substrate_name: NADH

substrate: NADH


organism: Hordeum vulgare


enzyme_display_name: dihydrofolate reductase

organism: Babesia gibsoni

proof_lines:
25°C, recombinant GST-tagged enzyme
25°C, recombinant detagged enzyme

enzyme: dihydrofolate reductase


substrate_name: NADPH

assay_conditions_summary: 298.15 K; cofactor NADPH

proof_lines:
25°C, recombinant detagged enzyme
25°C, recombinant GST-tagged enzyme

substrate: NADPH


organism: Bombyx mori


organism: Halalkalibacterium halodurans


proof_lines: His-tagged recombinant enzyme, pH 7.5, 37°C


assay_conditions_summary: pH 7.5; 310.15 K; cofactor NADPH


organism: Leishmania tropica


assay_conditions_summary: cofactor NADPH


organism: Moritella yayanosii

assay_conditions_summary: pH 7; 298.15 K; cofactor NADPH

proof_lines: pH 7.0, 25°C, under atmospheric pressure


substrate_name: dihydrofolate

substrate: dihydrofolate


organism: Schistosoma mansoni

substrate_name: NADP+

assay_conditions_summary: pH 7.8; 298.15 K; cofactor NADP+

substrate: NADP+


organism: Shewanella violacea


enzyme_display_name: FAD reductase (NADH)

organism: Paraburkholderia phenoliruptrix

substrate_name: FAD

assay_conditions_summary: pH 7; 297.15 K; cofactor NADH

enzyme: FAD reductase (NADH)

substrate: FAD


assay_conditions_summary: pH 7; 297.15 K; cofactor FAD

proof_lines:
cosubstrate: FMN, pH 7.0, 24°C
cosubstrate: FAD, pH 7.0, 24°C


substrate_name: FMN

substrate: FMN


organism: Clostridium cylindrosporum


organism: Photobacterium phosphoreum

proof_lines: bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase enzyme


enzyme_display_name: formyltetrahydrofolate dehydrogenase

organism: Danio rerio

proof_lines: 30°C, pH not specified in the publication, full-length recombinant enzyme

proof_lines: 30°C, pH not specified in the publication, recombinant N-terminal domain

enzyme: formyltetrahydrofolate dehydrogenase


enzyme_display_name: saccharopine dehydrogenase (NAD(+), L-lysine-forming)

organism: Phycomyces blakesleeanus

assay_conditions_summary: cofactor NADH


organism: Bacillus sp. BSD-8

substrate_name: sarcosine

smiles: CNCC(=O)O

proof_lines: 37°C, pH 8.0, poly-lysine modified enzyme

substrate: sarcosine


organism: Thermomicrobium roseum

proof_lines: wild-type, 37°C, pH not specified in the publication


assay_conditions_summary: 310.15 K; cofactor FAD

proof_lines: wild-type, reconstituted in presence of FAD, pH not specified in the publication, 37°C

proof_lines: wild-type, reconstituted in presence of 7-methyl-8-bromo-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C

proof_lines: wild-type, reconstituted in presence of 7,8-diethyl-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C


substrate_name: N-Methyl-L-alanine

smiles: CC(C(=O)O)NC

substrate: N-Methyl-L-alanine


substrate_name: N-methyl-L-tryptophan

substrate: N-methyl-L-tryptophan


substrate_name: N-methyl-D-proline

substrate: N-methyl-D-proline


substrate_name: N-methyl-L-phenylalanine

substrate: N-methyl-L-phenylalanine


substrate_name: N-methyl-L-aspartate

smiles: CNC(CC(=O)O)C(=O)O

substrate: N-methyl-L-aspartate


substrate_name: N-Methyl-L-leucine

smiles: CC(C)CC(C(=O)O)NC

substrate: N-Methyl-L-leucine


enzyme_display_name: spermine oxidase

organism: Arabidopsis thaliana

smiles: CC(=O)NCCCNCCCCNCCCN

proof_lines:
pH 7.5, 25°C, recombinant AtPAO4
pH 8.0
pH 8.0, 25°C, recombinant AtPAO1

enzyme: spermine oxidase


substrate_name: thermospermine

smiles: C(CCNCCCNCCCN)CN

proof_lines:
pH 8.0, 25°C, recombinant AtPAO1
pH 7.5, 25°C, recombinant AtPAO4

substrate: thermospermine


substrate_name: norspermine

smiles: C(CN)CNCCCNCCCN

proof_lines:
pH 8.0, 25°C, recombinant AtPAO1
pH 7.5, 25°C, recombinant AtPAO4
pH 8.0

substrate: norspermine


substrate_name: spermidine

smiles: C(CCNCCCN)CN

proof_lines:
pH 7.5, 25°C, recombinant AtPAO4
pH 8.0, substrate: spermine

substrate: spermidine


proof_lines: pH 7.5, 25°C, cosubstrate: N1-acetylspermine

proof_lines: pH 7.5, 25°C, cosubstrate: thermospermine


substrate_name: Unknown

substrate: Unknown


enzyme_display_name: non-specific polyamine oxidase

proof_lines:
37°C, pH 7.0
pH 7.5, 25°C, recombinant AtPAO3
37°C, pH 6.5

enzyme: non-specific polyamine oxidase


proof_lines:
37°C, pH 7.5
37°C, pH 7.0
pH 7.5, 37°C, recombinant AtPAO3


substrate_name: spermine

smiles: C(CCNCCCN)CNCCCN

substrate: spermine


proof_lines:
37°C, pH 6.5
37°C, pH 7.5
pH 7.5, 25°C, recombinant AtPAO3


proof_lines:
37°C, pH 6.5
37°C, pH 7.5
pH 7.5, 25°C, recombinant AtPAO2


smiles: CC(=O)NCCCNCCCCN

proof_lines:
37°C, pH 7.0
pH 7.5, 37°C, recombinant AtPAO3


enzyme_display_name: dimethylglycine dehydrogenase

organism: Homo sapiens

substrate_name: oxidized 2,6-dichlorophenolindophenol

enzyme: dimethylglycine dehydrogenase

substrate: oxidized 2,6-dichlorophenolindophenol


substrate_name: Ferrocene

substrate: Ferrocene


substrate_name: N,N-dimethylglycine

smiles: CN(C)CC(=O)O

proof_lines: 25°C, pH 7.8, artificial electron acceptor: 2,6-dichlorophenolindophenol, mutant enzyme H109R

proof_lines: mutant enzyme H109R, with 2,6-dichlorophenolindophenol as cosubstrate, at 25°C and pH 7.8 in 50 mM HEPES/NaOH with 150 mM NaCl

substrate: N,N-dimethylglycine


proof_lines: wild-type, presence of tetrahydrofolate, pH 7.5, 25°C

proof_lines: wild type enzyme, with 2,6-dichlorophenolindophenol as cosubstrate, at 25°C and pH 7.8 in 50 mM HEPES/NaOH with 150 mM NaCl


organism: Methanopyrus kandleri


substrate_name: reduced coenzyme F420

substrate: reduced coenzyme F420


enzyme_display_name: methylglutamate dehydrogenase

organism: Pseudomonas sp.

substrate_name: phenazine methosulfate

enzyme: methylglutamate dehydrogenase

substrate: phenazine methosulfate


substrate_name: N-Methyl-L-glutamate

smiles: CNC(CCC(=O)O)C(=O)O

substrate: N-Methyl-L-glutamate


substrate_name: 2-(methylamino)acetic acid

substrate: 2-(methylamino)acetic acid


substrate_name: N-methyl DL-valine

substrate: N-methyl DL-valine


substrate_name: N-methyl L-isoleucine

smiles: CCC(C)C(C(=O)O)NC

substrate: N-methyl L-isoleucine


substrate_name: potassium ferricyanide

substrate: potassium ferricyanide


substrate_name: N-methyl L-alanine

substrate: N-methyl L-alanine


enzyme_display_name: NADPH--hemoprotein reductase

organism: Anopheles minimus

substrate_name: NADH

assay_conditions_summary: pH 7.7; 298.15 K; cofactor NADPH

proof_lines: pH 7.7, 25°C, NADPH-cytochrome c oxidoreductase lacking the first 55 amino acid residues (DELTA55AnCY)

enzyme: NADPH--hemoprotein reductase

substrate: NADH


proof_lines: pH 7.7, 25°C, triple mutant L86F/L219F/P456A of NADPH-cytochrome c oxidoreductase lacking the first 55 amino acid residues (DELTA55AnCY)


substrate_name: NADPH

substrate: NADPH


substrate_name: ferricytochrome c

substrate: ferricytochrome c


proof_lines: wild type enzyme, pH not specified in the publication, at 25°C


assay_conditions_summary: 298.15 K; cofactor NADH


organism: Catharanthus roseus


organism: Cavia porcellus

substrate_name: menadione

substrate: menadione


organism: Dioscorea zingiberensis

assay_conditions_summary: pH 7.6; 298.15 K; cofactor NADPH


organism: Oryctolagus cuniculus


organism: Withania somnifera

assay_conditions_summary: pH 7.8; 298.15 K; cofactor NADPH

proof_lines: purified recombinant enzyme, pH 7.8, 25°C


enzyme_display_name: NADPH dehydrogenase (quinone)

enzyme: NADPH dehydrogenase (quinone)


substrate_name: Unknown

substrate: Unknown


enzyme_display_name: NAD(P)H dehydrogenase (quinone)

organism: Cupriavidus pinatubonensis JMP134

enzyme: NAD(P)H dehydrogenase (quinone)


substrate_name: FMN

assay_conditions_summary: cofactor FMN

substrate: FMN


organism: Arum maculatum

assay_conditions_summary: pH 7.2; 298.15 K; cofactor NADH


enzyme_display_name: nitrate reductase (NADH)

enzyme: nitrate reductase (NADH)


substrate_name: ferricyanide

substrate: ferricyanide


proof_lines: 22°C, pH not specified in the publication


substrate_name: nitrate

proof_lines: N-terminal plus C-terminal fragment, pH 7, 22°C

substrate: nitrate


organism: Emiliania huxleyi

assay_conditions_summary: pH 8.5; 298.15 K; cofactor NADH


enzyme_display_name: preQ1 synthase

organism: Pectobacterium carotovorum subsp. carotovorum

proof_lines:
pH 7.4, 30°C
below 0.25 microM, pH 7.4, 30°C

enzyme: preQ1 synthase


assay_conditions_summary: pH 7.4; 303.15 K; cofactor NADPH


enzyme_display_name: nitrate reductase (NADPH)

organism: Aspergillus nidulans

assay_conditions_summary: pH 6.5; cofactor NADPH

proof_lines: + nitrate, pH 6.5 and + cytochrome c, pH 7.5

enzyme: nitrate reductase (NADPH)


substrate_name: cytochrome c

assay_conditions_summary: pH 8.5; cofactor NADPH

proof_lines: NADPH as substrate in saturating and non-saturating concentrations, competitive

substrate: cytochrome c


assay_conditions_summary: pH 7.5; cofactor NADPH

proof_lines:
cosubstrate NADPH, pH 7.5
cosubstrate NADPH, pH 6.5
cosubstrate NADPH


assay_conditions_summary: cofactor NADPH


organism: Neurospora crassa


proof_lines: recombinant holo-enzyme, pH 7.5, temperature not specified in the publication


enzyme_display_name: azobenzene reductase

organism: Bacillus sp. B29

enzyme: azobenzene reductase


substrate_name: Methyl red

substrate: Methyl red


enzyme_display_name: nitrite reductase (NO-forming)

organism: Cereibacter sphaeroides

substrate_name: ferrocytochrome V(gamma)

enzyme: nitrite reductase (NO-forming)

substrate: ferrocytochrome V(gamma)


enzyme_display_name: nitrous-oxide reductase

organism: Paracoccus denitrificans

substrate_name: reduced benzyl viologen

enzyme: nitrous-oxide reductase

substrate: reduced benzyl viologen


substrate_name: reduced methyl viologen

substrate: reduced methyl viologen


enzyme_display_name: nitric-oxide reductase (cytochrome c)

organism: Schinkia azotoformans

substrate_name: NO

smiles: [No]

enzyme: nitric-oxide reductase (cytochrome c)

substrate: NO


enzyme_display_name: adenylyl-sulfate reductase

organism: Desulfobulbus propionicus

substrate_name: sulfite

proof_lines:
reaction with cytochrome c
reaction with ferricyanide

enzyme: adenylyl-sulfate reductase

substrate: sulfite


substrate_name: AMP

proof_lines:
reaction with ferricyanide
reaction with cytochrome c

substrate: AMP


organism: Homo sapiens

substrate_name: N-isovaleryl-S-farnesyl-L-cysteine

substrate: N-isovaleryl-S-farnesyl-L-cysteine


substrate_name: N-(benzoylglycyl)-S-farnesyl-L-cysteine

substrate: N-(benzoylglycyl)-S-farnesyl-L-cysteine


substrate_name: N-isobutyryl-S-farnesyl-L-cysteine

substrate: N-isobutyryl-S-farnesyl-L-cysteine


substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


substrate_name: N-acetyl-S-(E,E)-farnesyl-L-cysteine

proof_lines:
at pH 7.5 and 25°C
enzyme from endometrial carcinoma
enzyme from endometrium

substrate: N-acetyl-S-(E,E)-farnesyl-L-cysteine


substrate_name: biotin-S-farnesyl-L-cysteine

proof_lines: pH and temperature not specified in the publication

substrate: biotin-S-farnesyl-L-cysteine


substrate_name: N-acetyl-S-geranylgeranyl-L-cysteine

substrate: N-acetyl-S-geranylgeranyl-L-cysteine


organism: Mus musculus


enzyme_display_name: 24-methylenesterol C-methyltransferase

organism: Chlamydomonas reinhardtii

substrate_name: 24(28)-methylene cycloartenol

enzyme: 24-methylenesterol C-methyltransferase

substrate: 24(28)-methylene cycloartenol


substrate_name: obtusifoliol

substrate: obtusifoliol


substrate_name: 24(28)-methylene lophenol

substrate: 24(28)-methylene lophenol


substrate_name: lanosterol

substrate: lanosterol


substrate_name: Cycloartenol

substrate: Cycloartenol


enzyme_display_name: demethylspheroidene O-methyltransferase

organism: Rhodobacter capsulatus

enzyme: demethylspheroidene O-methyltransferase


enzyme_display_name: norbelladine O-methyltransferase

organism: Lycoris radiata

proof_lines:
pH 9.0, 37°C, product: isovanillin
pH 9.0, 37°C, product: vanillin

enzyme: norbelladine O-methyltransferase


substrate_name: caffeic acid

proof_lines:
pH 9.0, 37°C, product: isoferulic acid
pH 9.0, 37°C, product: ferulic acid

substrate: caffeic acid


substrate_name: norbelladine

proof_lines: pH 9.0, 37°C, product: 3'-O-methylnorbelladine

proof_lines: pH 9.0, 37°C, product: 4'-O-methylnorbelladine

substrate: norbelladine


organism: Humulus lupulus

substrate_name: desmethylxanthohumol

substrate: desmethylxanthohumol


substrate_name: Unknown

substrate: Unknown


enzyme_display_name: 3,5,7-trioxododecanoyl-CoA synthase

organism: Cannabis sativa

substrate_name: butyryl-CoA

assay_conditions_summary: pH 5.5; 303.15 K; cofactor CoA

proof_lines: pH 5.5, 30°C, dependent on product formed

enzyme: 3,5,7-trioxododecanoyl-CoA synthase

substrate: butyryl-CoA


substrate_name: hexanoyl-CoA

substrate: hexanoyl-CoA


enzyme_display_name: 4-hydroxycoumarin synthase

organism: Sorbus aucuparia

assay_conditions_summary: pH 6.5; 308.15 K; cofactor CoA

enzyme: 4-hydroxycoumarin synthase


substrate_name: malonyl-CoA

substrate: malonyl-CoA


enzyme_display_name: 2-heptyl-4(1H)-quinolone synthase

organism: Pseudomonas aeruginosa

assay_conditions_summary: pH 7; 310.15 K; cofactor CoA

enzyme: 2-heptyl-4(1H)-quinolone synthase


smiles: CCCCCCCC(=O)CC(=O)[O-]


substrate_name: malonyl-[acyl-carrier protein]

assay_conditions_summary: pH 7.5; 310.15 K; cofactor CoA

substrate: malonyl-[acyl-carrier protein]


enzyme_display_name: phloroglucinol synthase

organism: Pseudomonas fluorescens

assay_conditions_summary: cofactor CoA

proof_lines: wild type enzyme, pH and temperature not specified in the publication

enzyme: phloroglucinol synthase


proof_lines: mutant enzyme K210L, pH and temperature not specified in the publication


substrate_name: anthraniloyl-CoA

substrate: anthraniloyl-CoA


enzyme_display_name: 2-amino-4-oxopentanoate thiolase

organism: Acetoanaerobium sticklandii

smiles: CC(=O)CC(C(=O)O)N

assay_conditions_summary: pH 8

proof_lines: pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate

enzyme: 2-amino-4-oxopentanoate thiolase


substrate_name: CoA

assay_conditions_summary: pH 8; 299.15 K; cofactor CoA

substrate: CoA


enzyme_display_name: ethanol O-acetyltransferase

organism: Hanseniaspora uvarum

substrate_name: 4-nitrophenyl hexanoate

proof_lines: recombinant wild-type enzyme, pH 7.5, 35°C

enzyme: ethanol O-acetyltransferase

substrate: 4-nitrophenyl hexanoate


substrate_name: 4-nitrophenyl butyrate

substrate: 4-nitrophenyl butyrate


substrate_name: 4-nitrophenyl acetate

substrate: 4-nitrophenyl acetate


enzyme_display_name: aminoglycoside 6'-N-acetyltransferase

organism: Staphylococcus epidermidis

substrate_name: tobramycin

enzyme: aminoglycoside 6'-N-acetyltransferase

substrate: tobramycin


substrate_name: amikacin

substrate: amikacin


substrate_name: gentamicin

substrate: gentamicin


substrate_name: netilmicin

proof_lines:
pH 7.5, 37°C
pH 6.5, 37°C
pH 5.5, 37°C, substrate inhibition

substrate: netilmicin


enzyme_display_name: fatty-acid synthase system

assay_conditions_summary: pH 7; 298.15 K; cofactor CoA

proof_lines:
mutant enzyme K1699Q
25°C, pH 7.0, mutant enzyme K1699Q

enzyme: fatty-acid synthase system


substrate_name: acetyl-CoA

substrate: acetyl-CoA


proof_lines:
mutant enzyme K1699A
25°C, pH 7.0, mutant enzyme K1699A


proof_lines:
wild-type enzyme
25°C, pH 7.0, wild-type enzyme


substrate_name: NADPH

assay_conditions_summary: pH 7; 298.15 K; cofactor NADPH

substrate: NADPH


organism: Leishmania major

substrate_name: myristoyl-CoA

substrate: myristoyl-CoA


substrate_name: GQLFTSLN

substrate: GQLFTSLN


enzyme_display_name: hydroxymethylglutaryl-CoA synthase

organism: Blattella germanica

enzyme: hydroxymethylglutaryl-CoA synthase


substrate_name: acetoacetyl-CoA

substrate: acetoacetyl-CoA


enzyme_display_name: 2-methylcitrate synthase

organism: Rhodothermus marinus

substrate_name: oxaloacetate

enzyme: 2-methylcitrate synthase

substrate: oxaloacetate


substrate_name: propionyl-CoA

substrate: propionyl-CoA