enzyme_display_name: 2-amino-5-chlorophenol 1,6-dioxygenase

organism: Comamonas testosteroni

enzyme: 2-amino-5-chlorophenol 1,6-dioxygenase


source_db: brenda

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

protein_accession_database: UniProt

source_license: CC BY 4.0

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

source: brenda


organism: Streptosporangium sibiricum


enzyme_display_name: (R)-2-methylmalate dehydratase

organism: Methanocaldococcus jannaschii

substrate_name: H2O; Citraconate

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

enzyme: (R)-2-methylmalate dehydratase

substrate: H2O; Citraconate


enzyme_display_name: ADP-glyceromanno-heptose 6-epimerase

organism: Escherichia coli

substrate_name: ADP-D-glycero-D-manno-heptose

km_display: Not reported

assay_conditions_summary: cofactor ADP

enzyme: ADP-glyceromanno-heptose 6-epimerase

substrate: ADP-D-glycero-D-manno-heptose


substrate_name: 7,8-Dihydroneopterin 3'-triphosphate

substrate: 7,8-Dihydroneopterin 3'-triphosphate


enzyme_display_name: anthranilate 1,2-dioxygenase (deaminating, decarboxylating)

organism: Pseudomonas chlororaphis subsp. aureofaciens

substrate_name: anthranilate

substrate: anthranilate


enzyme_display_name: N-methylcoclaurine 3'-monooxygenase

organism: Eschscholzia californica

substrate_name: (S)-N-methylcoclaurine

enzyme: N-methylcoclaurine 3'-monooxygenase

substrate: (S)-N-methylcoclaurine


enzyme_display_name: (S)-limonene 7-monooxygenase

organism: Perilla frutescens

substrate_name: (-)-limonene

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

enzyme: (S)-limonene 7-monooxygenase

substrate: (-)-limonene


organism: Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

source_db: uniprot

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: KM values measured using the AntAB complex.

enzyme: anthranilate 1,2-dioxygenase (deaminating, decarboxylating)

source: uniprot


proof_lines: kcat is 88 min(-1) with SibE-PCP-3H4MAA as substrate.


enzyme_display_name: oryzalexin E synthase

organism: Oryza sativa subsp. japonica

substrate_name: ent-sandaracopimaradien

proof_lines: kcat is 0.046 sec(-1) with ent-sandaracopimaradien as substrate. kcat is 0.020 sec(-1) with syn-stemodene as substrate.

enzyme: oryzalexin E synthase

substrate: ent-sandaracopimaradien


substrate_name: 7,8-dihydroneopterin 3'-triphosphate

substrate: 7,8-dihydroneopterin 3'-triphosphate


enzyme_display_name: ent-isokaurene C2/C3-hydroxylase

organism: Oryza sativa

substrate_name: ent-isokaurene

proof_lines: pH and temperature not specified in the publication

enzyme: ent-isokaurene C2/C3-hydroxylase

substrate: ent-isokaurene


enzyme_display_name: ent-cassadiene hydroxylase

organism: Oryza sativa Japonica Group

proof_lines: pH not specified in the publication, temperature not specified in the publication

enzyme: ent-cassadiene hydroxylase


substrate_name: ent-cassadiene

proof_lines: kcat is 0.11 sec(-1) with ent-cassadiene as substrate.

substrate: ent-cassadiene


proof_lines: kcat is 0.01 sec(-1) with ent-isokaurene as substrate.


organism: Rhodococcus erythropolis

substrate_name: DTDB

substrate: DTDB


enzyme_display_name: plasminogen activator Pla

organism: Yersinia pestis

substrate_name: plasminogen

enzyme: plasminogen activator Pla

substrate: plasminogen


enzyme_display_name: pheophorbide a oxygenase

organism: Arabidopsis thaliana

substrate_name: pheophorbide a

enzyme: pheophorbide a oxygenase

substrate: pheophorbide a


enzyme_display_name: ferredoxin:thioredoxin reductase

organism: Spinacia oleracea

substrate_name: reduced ferredoxin

proof_lines: ferredoxin modified by treatment with glycine ethyl ester in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, pH 7.9

enzyme: ferredoxin:thioredoxin reductase

substrate: reduced ferredoxin


enzyme_display_name: (-)-pluviatolide 4-O-methyltransferase

organism: Sinopodophyllum hexandrum

substrate_name: (-)-pluviatolide

enzyme: (-)-pluviatolide 4-O-methyltransferase

substrate: (-)-pluviatolide


enzyme_display_name: arginine N(omega)-methyltransferase

organism: Streptomyces achromogenes subsp. streptozoticus

substrate_name: L-arginine

enzyme: arginine N(omega)-methyltransferase

substrate: L-arginine


enzyme_display_name: sesamin methylene transferase

organism: Sinomonas sp. No.22

substrate_name: (+)-sesamin

enzyme: sesamin methylene transferase

substrate: (+)-sesamin


organism: Mus musculus

substrate_name: docosahexaenoyl-CoA

assay_conditions_summary: cofactor CoA

substrate: docosahexaenoyl-CoA


enzyme_display_name: tyramine N-methyltransferase

organism: Hordeum vulgare

substrate_name: S-adenosyl-L-methionine

enzyme: tyramine N-methyltransferase

substrate: S-adenosyl-L-methionine


km_unit: mM

data_origin: experimental


organism: Coptis japonica

substrate_name: columbamine

substrate: columbamine


organism: Homo sapiens

substrate_name: lysophosphatidylethanolamine

substrate: lysophosphatidylethanolamine


enzyme_display_name: GDP-perosamine N-acetyltransferase

organism: Escherichia coli O157:H7

substrate_name: GDP-perosamine

enzyme: GDP-perosamine N-acetyltransferase

substrate: GDP-perosamine


organism: Petroselinum crispum


organism: Methanocaldococcus jannaschii

substrate_name: Unknown

ki_unit: mM

substrate: Unknown


enzyme_display_name: phosphorylated butenolide synthase

organism: Streptomyces griseus

substrate_name: glycerone phosphate

enzyme: phosphorylated butenolide synthase

substrate: glycerone phosphate


enzyme_display_name: capsaicin synthase

organism: Capsicum annuum

assay_conditions_summary: pH 6.8; 310.15 K; cofactor CoA

enzyme: capsaicin synthase


organism: Streptomyces kanamyceticus


organism: Thermotoga maritima

substrate_name: GDP-alpha-D-mannose

substrate: GDP-alpha-D-mannose


enzyme_display_name: (Kdo)-lipid IVA 3-deoxy-D-manno-octulosonic acid transferase

organism: Escherichia coli


enzyme_display_name: homogentisate phytyltransferase

substrate_name: Phytyl diphosphate; Homogentisate

enzyme: homogentisate phytyltransferase

substrate: Phytyl diphosphate; Homogentisate


organism: Synechocystis sp.


organism: Gallus gallus

substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


enzyme_display_name: histidine N-acetyltransferase

organism: Oreochromis niloticus

substrate_name: acetylcoenzyme A

enzyme: histidine N-acetyltransferase

substrate: acetylcoenzyme A


enzyme_display_name: lysyltransferase

organism: Staphylococcus aureus

substrate_name: phosphatidylglycerol

enzyme: lysyltransferase

substrate: phosphatidylglycerol


enzyme_display_name: diglucosyl diacylglycerol synthase (1,2-linking)

organism: Acholeplasma laidlawii

substrate_name: UDP-glucose

substrate: UDP-glucose


enzyme: diglucosyl diacylglycerol synthase (1,2-linking)


substrate_name: UDP-alpha-D-galactose

substrate: UDP-alpha-D-galactose


organism: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

substrate_name: fructose-1,6-bisphosphate (at pH 7 and 50 degrees Celsius)


substrate_name: acetyl-CoA

substrate: acetyl-CoA


organism: Staphylococcus aureus (strain NCTC 8325 / PS 47)

substrate_name: L-lysyl-tRNA

substrate: L-lysyl-tRNA


substrate_name: UDP-Glc (alpha-diglucosyldiacylglycerol synthase activity at 28 degrees Celsius)


substrate_name: UDP-N-acetyl-alpha-D-glucosamine

substrate: UDP-N-acetyl-alpha-D-glucosamine


substrate_name: UDP-Gal

substrate: UDP-Gal


organism: Shigella boydii


organism: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

substrate_name: DIP

substrate: DIP


enzyme_display_name: xylosyl alpha-1,3-xylosyltransferase

organism: Homo sapiens

substrate_name: UDP-alpha-D-xylose

enzyme: xylosyl alpha-1,3-xylosyltransferase

substrate: UDP-alpha-D-xylose


organism: Escherichia coli (strain K12)

substrate_name: lipid IV(A) (at pH 8)

enzyme: (Kdo)-lipid IVA 3-deoxy-D-manno-octulosonic acid transferase

substrate: lipid IV(A) (at pH 8)


organism: Bacillus subtilis

substrate_name: UDPglucose

substrate: UDPglucose


substrate_name: UDP-D-xylose

substrate: UDP-D-xylose


organism: Sinopodophyllum hexandrum

substrate_name: (-)-pluviatolide

proof_lines: kcat is 0.72 sec(-1) with (-)-pluviatolide as substrate.

substrate: (-)-pluviatolide


organism: Sinomonas sp. (strain No.22)

substrate_name: sesamin

proof_lines: kcat is 7.9 sec(-1) with sesamin as substrate.

substrate: sesamin


organism: Streptomyces griseus subsp. griseus (strain JCM 4626 / CBS 651.72 / NBRC 13350 / KCC S-0626 / ISP 5235)

substrate_name: DHAP

substrate: DHAP


organism: Bacillus subtilis (strain 168)

substrate_name: UDP-alpha-D-glucose

substrate: UDP-alpha-D-glucose


organism: Cannabis sativa

substrate_name: olivetolic acid

substrate: olivetolic acid


organism: Mycobacterium tuberculosis

assay_conditions_summary: cofactor ATP


enzyme_display_name: tagatose kinase

organism: Agrobacterium fabrum (strain C58 / ATCC 33970)

substrate_name: D-tagatose

enzyme: tagatose kinase

substrate: D-tagatose


organism: Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)

substrate_name: D-glycero-D-manno-heptose 7-phosphate (at pH 7.4 and 30 degrees Celsius)

proof_lines: kcat is 0.48 sec(-1) for D-glycero-D-manno-heptose 7-phosphate.


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: ATP

proof_lines: kcat is 104 min(-1) for ATPase activity.

substrate: ATP


enzyme_display_name: quinolinate synthase

substrate_name: dihydroxyacetone phosphate

enzyme: quinolinate synthase

substrate: dihydroxyacetone phosphate


organism: Mycobacterium butyricum

assay_conditions_summary: pH 7.5; 295.15 K; cofactor ATP


substrate_name: olivetolate

substrate: olivetolate


enzyme_display_name: diacylglycerol kinase (CTP)

organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: CTP

enzyme: diacylglycerol kinase (CTP)

substrate: CTP


enzyme_display_name: spermosin

organism: Unknown

enzyme: spermosin


enzyme_display_name: mevalonate-3-phosphate 5-kinase

organism: Thermoplasma acidophilum

enzyme: mevalonate-3-phosphate 5-kinase


enzyme_display_name: pyridinol guanylyltransferase

organism: Methanocaldococcus jannaschii

substrate_name: GTP

proof_lines: with 3,6-dimethyl-2,4-dihydroxypyridine as cosubstrate, at pH 7.0 and 80°C

enzyme: pyridinol guanylyltransferase

substrate: GTP


enzyme_display_name: isoamyl acetate esterase

organism: Saccharomyces cerevisiae

substrate_name: 3-methylbutyl acetate

enzyme: isoamyl acetate esterase

substrate: 3-methylbutyl acetate


enzyme_display_name: carbendazim hydrolysing esterase

organism: Nocardioides sp. SG-4G

substrate_name: carbendazim

enzyme: carbendazim hydrolysing esterase

substrate: carbendazim


enzyme_display_name: maltose 6'-phosphate phosphatase

organism: Enterococcus faecalis

substrate_name: maltose 6'-phosphate

assay_conditions_summary: pH 7

proof_lines: pH 7.0, temperature not specified in the publication

enzyme: maltose 6'-phosphate phosphatase

substrate: maltose 6'-phosphate


enzyme_display_name: galactan exo-1,6-beta-galactobiohydrolase (non-reducing end)

organism: Bifidobacterium longum subsp. Longum


organism: Pantoea agglomerans

proof_lines: 30°C, pH not specified in the publication


enzyme_display_name: 8-oxo-(d)GTP phosphatase

enzyme: 8-oxo-(d)GTP phosphatase


enzyme_display_name: 1'-carboxy-chondrochloren decarboxylase

organism: Chondromyces crocatus

substrate_name: 1'-carboxy-chondrochloren B

enzyme: 1'-carboxy-chondrochloren decarboxylase

substrate: 1'-carboxy-chondrochloren B


enzyme_display_name: geosmin synthase

organism: Streptomyces avermitilis

substrate_name: (2E,6E)-farnesyl diphosphate

enzyme: geosmin synthase

substrate: (2E,6E)-farnesyl diphosphate


enzyme_display_name: 3-amino-4-hydroxybenzoate synthase

organism: Streptomyces griseus

enzyme: 3-amino-4-hydroxybenzoate synthase


enzyme_display_name: GDP-perosamine N-acetyltransferase

organism: Escherichia coli

substrate_name: acetyl-CoA

assay_conditions_summary: pH 8; 310.15 K; cofactor CoA

enzyme: GDP-perosamine N-acetyltransferase

substrate: acetyl-CoA


enzyme_display_name: catalase peroxidase

organism: Mycobacterium tuberculosis

mutation_type: substitution

enzyme: catalase peroxidase


substrate_name: D-glucitol[side 1]

substrate: D-glucitol[side 1]


organism: Escherichia coli (strain K12)

substrate_name: D-galactitol

substrate: D-galactitol


enzyme_display_name: L-fuculokinase

substrate_name: L-fuculose

enzyme: L-fuculokinase

substrate: L-fuculose


enzyme_display_name: farnesyl-diphosphate kinase

organism: Bos taurus

enzyme: farnesyl-diphosphate kinase


organism: Homo sapiens

substrate_name: IDP

substrate: IDP


enzyme_display_name: oxepin-CoA hydrolase

substrate_name: oxepin-CoA (at 22 degrees Celsius and pH 8)

assay_conditions_summary: pH 8; cofactor CoA

enzyme: oxepin-CoA hydrolase


enzyme_display_name: Xaa-Arg dipeptidase

substrate_name: beta-alanyl-L-lysine

enzyme: Xaa-Arg dipeptidase

substrate: beta-alanyl-L-lysine


organism: Mus musculus


enzyme_display_name: tartrate decarboxylase

organism: Pseudomonas putida

substrate_name: D-malate

enzyme: tartrate decarboxylase

substrate: D-malate


organism: Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

substrate_name: FPP

substrate: FPP


enzyme_display_name: dimethylmaleate hydratase

organism: Eubacterium barkeri

substrate_name: dimethylmaleate

enzyme: dimethylmaleate hydratase

substrate: dimethylmaleate


organism: Citrobacter freundii

substrate_name: L-Fuculose

substrate: L-Fuculose


assay_conditions_summary: pH 7.8; 310.15 K; cofactor ATP


enzyme_display_name: mevalonate-3-phosphate 5-kinase

organism: Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

substrate_name: Unknown

enzyme: mevalonate-3-phosphate 5-kinase

substrate: Unknown


enzyme_display_name: pyridinol guanylyltransferase

organism: Methanococcus maripaludis (strain DSM 14266 / JCM 13030 / NBRC 101832 / S2 / LL)

enzyme: pyridinol guanylyltransferase


organism: Enterococcus faecalis (strain TX4000 / JH2-2)


organism: Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)

proof_lines: kcat is 59.1 sec(-1) for UDP-N,N'-diacetylbacillosamine (at pH 7.5 and 37 degrees Celsius).


organism: Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)

proof_lines: kcat is 261 sec(-1) with beta-1,6-Gal3 as substrate.

enzyme: galactan exo-1,6-beta-galactobiohydrolase (non-reducing end)


organism: Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1)

substrate_name: 8-oxo-dGTP (at pH 7.6 and 37 degrees Celsius as measured by the Pi sensor assay)

proof_lines: kcat is 0.18 sec(-1) with 8-oxo-dGTP as substrate measured by the Pi sensor assay. kcat is 0.29 sec(-1) with 8-oxo-dGTP as substrate measured by the Fiske-SubbaRow assay. kcat is 0.13 sec(-1) with 8-oxo-GTP as substrate.


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

proof_lines: kcat is 0.54 sec(-1) with 8-oxo-dGTP as substrate.


enzyme_display_name: pyrrole-2-carboxylate decarboxylase

organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

proof_lines: kcat is 35.8 sec(-1) with pyrrole-2-carboxylate as substrate.

enzyme: pyrrole-2-carboxylate decarboxylase


organism: Streptomyces griseus

substrate_name: 2-amino-4,5-dihydroxy-6-one-heptanoic acid-7-phosphate (at 30 degrees Celsius)

proof_lines: This Km value might be underestimated because the concentration of the substrate determined is perhaps lower than the actual concentration due to the instability of the substrate compound.


organism: Legionella pneumophila

substrate_name: UDP-N,N'-diacetylbacillosamine

substrate: UDP-N,N'-diacetylbacillosamine


organism: Fusarium fujikuroi

substrate_name: trans-farnesyl triphosphate

substrate: trans-farnesyl triphosphate


enzyme_display_name: spermosin

organism: Halocynthia roretzi

assay_conditions_summary: pH 8

enzyme: spermosin


organism: Sus scrofa


organism: Escherichia coli

substrate_name: oxepin-CoA

proof_lines: pH and temperature not specified in the publication

substrate: oxepin-CoA


enzyme_display_name: verruculogen synthase

organism: Aspergillus fumigatus

substrate_name: fumitremorgin B

enzyme: verruculogen synthase

substrate: fumitremorgin B


enzyme_display_name: 3-hydroxyanthranilate oxidase

organism: Rattus norvegicus

enzyme: 3-hydroxyanthranilate oxidase


enzyme_display_name: fatty-acid peroxidase

organism: Arachis hypogaea

substrate_name: palmitate

enzyme: fatty-acid peroxidase

substrate: palmitate


organism: Lathyrus oleraceus

substrate_name: lauric acid

substrate: lauric acid


organism: Pseudomonas sp.

substrate_name: L-Tartrate

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NAD+

substrate: L-Tartrate


enzyme_display_name: (Ara-f)3-Hyp beta-L-arabinobiosidase

organism: Bifidobacterium longum

proof_lines: in 50 mM sodium acetate (pH 6.0), at 37°C

enzyme: (Ara-f)3-Hyp beta-L-arabinobiosidase


organism: Priestia megaterium

substrate_name: Pyrrole

substrate: Pyrrole


organism: Eubacterium barkeri


enzyme_display_name: uronolactonase

substrate_name: H2O; D-Glucurono-3,6-lactone

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

enzyme: uronolactonase

substrate: H2O; D-Glucurono-3,6-lactone


enzyme_display_name: isobutylamine N-monooxygenase

organism: Streptomyces viridifaciens

proof_lines: pH 7.5, 25°C, flavin reductase(FRED)/isobutylamine N-hydroxylase(IBAH) system

enzyme: isobutylamine N-monooxygenase


organism: Oospora sulphurea-ochracea


enzyme_display_name: anthocyanidin synthase

organism: Gerbera hybrid cultivar

substrate_name: (+)-catechin

enzyme: anthocyanidin synthase

substrate: (+)-catechin


enzyme_display_name: androst-4-ene-3,17-dione monooxygenase

organism: Ilyonectria destructans

substrate_name: testololactone

assay_conditions_summary: cofactor NADPH

enzyme: androst-4-ene-3,17-dione monooxygenase

substrate: testololactone


enzyme_display_name: nitrogenase (flavodoxin)

organism: Azotobacter vinelandii

substrate_name: flavodoxin hydroquinone

proof_lines: before and after reduction of the nitrogenase complex relative slow reactions take place, which limits the rate of the Fe protein cycle

enzyme: nitrogenase (flavodoxin)

substrate: flavodoxin hydroquinone


enzyme_display_name: beta-cyclopiazonate dehydrogenase

organism: Penicillium cyclopium

enzyme: beta-cyclopiazonate dehydrogenase


enzyme_display_name: selenate reductase (quinol)

organism: Enterobacter cloacae

substrate_name: selenate

enzyme: selenate reductase (quinol)

substrate: selenate


enzyme_display_name: (-)-lariciresinol reductase

organism: Camellia sinensis var. sinensis

substrate_name: (-)-lariciresinol

enzyme: (-)-lariciresinol reductase

substrate: (-)-lariciresinol


enzyme_display_name: isoflavone 7-O-methyltransferase

organism: Glycine max

assay_conditions_summary: pH 7.6; 303.15 K; cofactor CoA

enzyme: isoflavone 7-O-methyltransferase


organism: Shigella boydii


organism: Escherichia coli

substrate_name: [pyruvate formate-lyase]-glycine

substrate: [pyruvate formate-lyase]-glycine


enzyme_display_name: 6-hydroxymellein O-methyltransferase

organism: Daucus carota

substrate_name: Unknown

enzyme: 6-hydroxymellein O-methyltransferase


substrate_name: cytidine34 in tRNALeuCAA

proof_lines: wild-type tRNALeuCAA and diverse mutants thereof, pH 7.5, 37°C

substrate: cytidine34 in tRNALeuCAA


organism: Bacillus subtilis

substrate_name: adenine22 in tRNASer

sequence_source: UniProt P54471 identity context

proof_lines: recombinant enzyme, pH 8.0, 37°C, in vivo activity

proof_lines: recombinant enzyme, pH 8.0, 37°C, in vitro activity

substrate: adenine22 in tRNASer


enzyme_display_name: tRNA:m(4)X modification enzyme

organism: Saccharomyces cerevisiae

substrate_name: adenosine4 in tRNAHis

sequence_source: UniProt Q12383 identity context

enzyme: tRNA:m(4)X modification enzyme

substrate: adenosine4 in tRNAHis


enzyme_display_name: GDP-perosamine N-formyltransferase

organism: Brucella melitensis

substrate_name: GDP-alpha-D-perosamine

sequence_source: UniProt Q9ZHX0 redirects to F8WJP6; identity context only

proof_lines: mutant enzyme D78A/F142A, at pH 8.0, temperature not specified in the publication

enzyme: GDP-perosamine N-formyltransferase

substrate: GDP-alpha-D-perosamine


assay_conditions_summary: pH 6.5; 308.15 K; cofactor CoA


enzyme_display_name: 2-phosphonomethylmalate synthase

organism: Streptomyces hygroscopicus

substrate_name: acetyl-CoA

enzyme: 2-phosphonomethylmalate synthase

substrate: acetyl-CoA


enzyme_display_name: mannosylglycerate hydrolase

organism: Rubrobacter radiotolerans

substrate_name: mannosylglycerate (at 50 degrees Celsius)

enzyme: mannosylglycerate hydrolase


organism: Selaginella moellendorffii

substrate_name: 2-O-(alpha-D-mannosyl)-glycerate (at 40 degrees Celsius)


organism: Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27)

substrate_name: mannosylglycerate (at 70 degrees Celsius)


organism: Lathyrus oleraceus

substrate_name: pyridoxamine

substrate: pyridoxamine


enzyme_display_name: luteolin 7-O-glucuronosyltransferase

organism: Secale cereale

substrate_name: UDPglucuronic acid

enzyme: luteolin 7-O-glucuronosyltransferase

substrate: UDPglucuronic acid


substrate_name: luteolin 7-O-glucuronide

substrate: luteolin 7-O-glucuronide


substrate_name: poly(ribitol phosphate)

proof_lines:
molar concentration of the polymer
concentration as ribitol units

substrate: poly(ribitol phosphate)


organism: Homo sapiens


organism: Rattus norvegicus


enzyme_display_name: arylamine glucosyltransferase

substrate_name: UDPglucose

enzyme: arylamine glucosyltransferase

substrate: UDPglucose


enzyme_display_name: kaempferol 3-O-xylosyltransferase

organism: Euonymus alatus

substrate_name: UDP-alpha-D-xylose

proof_lines:
pH 7.5, 30°C, cosubstrate: kaempferol
pH 7.5, 30°C

enzyme: kaempferol 3-O-xylosyltransferase

substrate: UDP-alpha-D-xylose


enzyme_display_name: aminodeoxyfutalosine synthase

organism: Thermus thermophilus

enzyme: aminodeoxyfutalosine synthase


enzyme_display_name: lombricine kinase

organism: Megascolides cameroni

substrate_name: D-lombricine

enzyme: lombricine kinase

substrate: D-lombricine


enzyme_display_name: thiamine-diphosphate kinase

organism: Bos taurus

substrate_name: ATP

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

enzyme: thiamine-diphosphate kinase

substrate: ATP


organism: Cavia porcellus


enzyme_display_name: serine-phosphoethanolamine synthase

organism: Gallus gallus

substrate_name: L-serine

enzyme: serine-phosphoethanolamine synthase

substrate: L-serine


enzyme_display_name: bis(2-ethylhexyl)phthalate esterase

organism: Achromobacter denitrificans

substrate_name: 4-nitrophenyl acetate

enzyme: bis(2-ethylhexyl)phthalate esterase

substrate: 4-nitrophenyl acetate


organism: Triticum aestivum


enzyme_display_name: glutathione thiolesterase

organism: Spinacia oleracea

substrate_name: S-butyrylglutathione

enzyme: glutathione thiolesterase

substrate: S-butyrylglutathione


substrate_name: D-serine-phosphoethanolamine

substrate: D-serine-phosphoethanolamine


enzyme_display_name: mannan exo-1,2-1,6-alpha-mannosidase

organism: Cellulomonas sp.

substrate_name: D-mannan

proof_lines: pH 7, 37°C, hydrolysis of alpha-1,2-linkage in yeast mannan

proof_lines: pH 7, 37°C, hydrolysis of alpha-1,6-linkage in yeast mannan

proof_lines: pH 7, 37°C, hydrolysis of alpha-1,3-linkage in yeast mannan

enzyme: mannan exo-1,2-1,6-alpha-mannosidase

substrate: D-mannan


enzyme_display_name: cyclic 2,3-diphosphoglycerate hydrolase

organism: Methanothermobacter thermautotrophicus

substrate_name: cyclic 2,3-bisphosphoglycerate

enzyme: cyclic 2,3-diphosphoglycerate hydrolase

substrate: cyclic 2,3-bisphosphoglycerate


organism: Alteromonas sp. SN-1009

substrate_name: Kjellmaniella crassifolia fucoidan

proof_lines:
pH 7.0, 35°C
kcat/Km: 0.21 ml/mg*min, pH 7.0, 35°C

substrate: Kjellmaniella crassifolia fucoidan


enzyme_display_name: Pup amidohydrolase

organism: Mycolicibacterium smegmatis

enzyme: Pup amidohydrolase


enzyme_display_name: firefly luciferase

organism: Photinus pyralis

substrate_name: D-luciferin

assay_conditions_summary: pH 8.6; 298.15 K; cofactor ATP

proof_lines:
wild-type enzyme
pH 8.6, wild-type enzyme
25°C, pH 8, wild-type

enzyme: firefly luciferase

substrate: D-luciferin


organism: Luciola mingrelica

substrate_name: luciferin

substrate: luciferin


organism: Lampyris turkestanicus

source_license: CC BY 4.0; CC BY-NC-ND 4.0

assay_conditions_summary: pH 7.8; buffer 50 mM Tricine-NaOH; cofactor ATP

proof_lines: at pH 7.8, in 50 mM Tricine-NaOH buffer with 10 mM MgSO4

proof_lines: in 50 mM Tricine-NaOH buffer with 10 mM MgSO4, at pH 7.8

source_databases_merged: brenda skid


proof_lines: pH and temperature not specified in the publication, wild-type enzyme


organism: Nipponoluciola cruciata

substrate_name: lauric acid

substrate: lauric acid


substrate_name: Dehydroluciferyladenylate; CTP

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

assay_conditions_summary: pH 7.5; 298.15 K; cofactor ATP

proof_lines:
pH 7.5, 25°C
native luciferase
recombinant luciferase

source_databases_merged: sabio_rk brenda skid

substrate: Dehydroluciferyladenylate; CTP


assay_conditions_summary: pH 7.8; 295.15 K; cofactor ATP

proof_lines: pH 7.8, temperature not specified in the publication, wild-type enzyme

proof_lines: pH 7.8, 22°C, recombinant wild-type enzyme


enzyme_display_name: phosphogluconate dehydratase

organism: Caulobacter vibrioides

enzyme: phosphogluconate dehydratase


enzyme_display_name: Transferred entry: 4.8.1.2

organism: Pseudomonas chlororaphis

substrate_name: n-Butyraldoxime

enzyme: Transferred entry: 4.8.1.2

substrate: n-Butyraldoxime


enzyme_display_name: 4,5-dihydroxyphthalate decarboxylase

organism: Comamonas testosteroni

enzyme: 4,5-dihydroxyphthalate decarboxylase


organism: Pseudomonas fluorescens


enzyme_display_name: ricinine nitrilase

organism: Pseudomonas sp.

enzyme: ricinine nitrilase


organism: Methylococcus capsulatus

substrate_name: D-Arabino-6-hexulose 3-phosphate

substrate: D-Arabino-6-hexulose 3-phosphate


organism: Pseudomonas putida


organism: Zymomonas mobilis


enzyme_display_name: L-rhamnose isomerase

organism: Stutzerimonas stutzeri

substrate_name: D-allose

assay_conditions_summary: pH 9; 308.15 K; buffer 50 mM glycine-NaOH

proof_lines: mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C

enzyme: L-rhamnose isomerase

substrate: D-allose


proof_lines: mutant enzyme H101N, pH and temperature not specified in the publication


organism: Thermoanaerobacterium saccharolyticum

substrate_name: L-Mannose

assay_conditions_summary: pH 7; 338.15 K; buffer and 1 mM Co2+ at pH 7

proof_lines: at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0

substrate: L-Mannose


organism: Thermobacillus composti


organism: Thermoclostridium stercorarium


enzyme_display_name: sucrose phosphorylase

organism: Bifidobacterium longum

substrate_name: phosphate

enzyme: sucrose phosphorylase

substrate: phosphate


organism: Leuconostoc mesenteroides

substrate_name: sucrose

proof_lines: pH 7.0, 30°C, phosphorolysis, recombinant enzyme

proof_lines: pH 7.0, 30°C, synthesis, recombinant enzyme

substrate: sucrose


organism: Leuconostoc mesenteroides subsp. mesenteroides


source_db: oed

source: oed


organism: Thermobacillus sp. ZCTH02-B1


organism: uncultured bacterium


organism: Bacillus cereus


organism: Lactiplantibacillus plantarum subsp. plantarum

substrate_name: isomaltose

proof_lines: pH 5.5, 30°C, recombinant His-tagged enzyme

substrate: isomaltose


organism: Saccharomyces cerevisiae

substrate_name: methyl alpha-D-glucopyranoside

proof_lines:
pH 7.0, 30°C
Raw SKiD Env: Temp=30°C, pH=7
Raw SKiD Env: Temp=30°C, pH=6.8

substrate: methyl alpha-D-glucopyranoside


organism: Suncus murinus

substrate_name: H2O; Isomaltose

substrate: H2O; Isomaltose


organism: Aeribacillus pallidus

substrate_name: L-rhamnose

substrate: L-rhamnose


organism: Bacillus subtilis


organism: Caldicellulosiruptor obsidiansis


organism: Caldicellulosiruptor saccharolyticus

assay_conditions_summary: pH 7; 338.15 K; buffer 50 mM potassium phosphate

proof_lines: in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 65°C


organism: Dictyoglomus turgidum


organism: Pseudomonas stutzeri

substrate_name: D-ribose

source_db: skid

source_license: CC BY-NC-ND 4.0

proof_lines:
Raw SKiD Env: Temp=35°C, pH=9
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=50°C, pH=9

substrate: D-ribose

source: skid


organism: Stutzerimonas stutzeri

proof_lines: 50°C, pH 9.0, intrinsic enzyme from Pseudomonas stutzeri


proof_lines: wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C

proof_lines: wild type enzyme, pH and temperature not specified in the publication


proof_lines: mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C


proof_lines: mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C


proof_lines: mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C


organism: Homo sapiens

substrate_name: maltose

substrate: maltose


organism: Rattus norvegicus

substrate_name: maltose (with stSI)

substrate: maltose (with stSI)


organism: Bifidobacterium breve

substrate_name: L-ascorbic acid

substrate: L-ascorbic acid


proof_lines: pH 4.5, 40°C, mutant enzyme P134C/L341V/L343F


organism: Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)

verification_status: corrected

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

proof_lines: kcat is 207 sec(-1) for the phosphorolysis of sucrose (at 58 degrees Celsius and pH 6.5).

status: corrected


organism: Bifidobacterium adolescentis


proof_lines: pH 7.0, 60°C, recombinant wild-type enzyme


organism: Leuconostoc mesenteroides


substrate_name: D-fructose

proof_lines:
30°C, pH 7.0, mutant enzyme D295E
pH 7.0, 30°C, synthesis, mutant D295E
pH 7.0, 30°C, arsenolysis, mutant D295E

substrate: D-fructose


substrate_name: alpha-D-glucose 1-phosphate

proof_lines: pH 7.0, 30°C, phosphorolysis, mutant D295N

substrate: alpha-D-glucose 1-phosphate


organism: Bifidobacterium dentium


proof_lines: pH 7.0, 30°C, phosphorolysis, mutant E237Q


proof_lines: arsenolysis of alpha-D-glucose 1-phosphate, mutant D196N/E237Q


proof_lines: pH 7.0, 30°C, sucrose phosphorolysis, recombinant wild-type enzyme

proof_lines: pH 7.0, 30°C, phosphorolysis, recombinant wild-type enzyme

proof_lines: pH 7.0, 30°C, sucrose synthesis by glycosylation from alpha-D-glucose 1-phosphate, recombinant wild-type enzyme


proof_lines: pH and temperature not specified in the publication


organism: Limosilactobacillus reuteri


organism: Roseateles saccharophilus


organism: Streptococcus mutans


organism: Zalophus californianus


organism: Pseudomonas sp.


substrate_name: L-Xylose

substrate: L-Xylose


organism: Thermotoga maritima

assay_conditions_summary: pH 8; 358.15 K; buffer 50 mM EPPS

proof_lines: purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)


organism: Bacillus subtilis

substrate_name: L-Lyxose

substrate: L-Lyxose


organism: Halalkalibacterium halodurans

assay_conditions_summary: pH 7; 343.15 K; buffer 50 mM malate

proof_lines: in 50 mM malate buffer, at pH 7.0 and 70°C


organism: Lactiplantibacillus plantarum


organism: Mesorhizobium loti

proof_lines: in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C


organism: Escherichia coli

substrate_name: L-Rhamnose

substrate: L-Rhamnose


enzyme_display_name: peptidylprolyl isomerase

organism: Arabidopsis thaliana

enzyme: peptidylprolyl isomerase


organism: Corynebacterium glutamicum

proof_lines: pH 7.5, temperature not specified in the publication


substrate_name: RNase T1

proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication

substrate: RNase T1


proof_lines: mutant Y15A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant I65P, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant A82P, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant V72P, pH not specified in the publication, temperature not specified in the publication


organism: Halobacterium salinarum


organism: Homo sapiens


substrate_name: Unknown

substrate: Unknown


organism: Thermus thermophilus

substrate_name: TRYWNAKMK-(cis)-PFIFGA

proof_lines: wild-type, pH 7.5, temperature not specified in the publication

substrate: TRYWNAKMK-(cis)-PFIFGA


proof_lines: mutant lacking the insert-in-flap domain, pH 7.5, temperature not specified in the publication


organism: Trypanosoma cruzi

assay_conditions_summary: pH 7.8; 283.15 K; buffer 35 mM sodium HEPES

proof_lines: at 10 °C in 35 mM sodium HEPES buffer, pH 7.8


enzyme_display_name: phenylpyruvate tautomerase

organism: Bos taurus

enzyme: phenylpyruvate tautomerase


substrate_name: keto-(p-hydroxyphenyl)pyruvate

substrate: keto-(p-hydroxyphenyl)pyruvate


organism: Mus musculus

proof_lines:
23°C, pH 6.5
Raw SKiD Env: Temp=23°C, pH=6.5
Raw SKiD Env: Temp=23°C, pH=-----


substrate_name: enol-(p-hydroxyphenyl)pyruvate

sequence_source: UniProtKB:P34884 (canonical identity context only)

proof_lines:
23°C, pH 6.5, mutant enzyme Y95F
23°C, mutant enzyme Y95F

substrate: enol-(p-hydroxyphenyl)pyruvate


substrate_name: enol-phenylpyruvate

proof_lines:
23°C, mutant enzyme P1A
23°C, pH 6.5, mutant enzyme P1A

substrate: enol-phenylpyruvate


proof_lines:
23°C, pH 6.5, mutant enzyme N97A
23°C, mutant enzyme N97A


proof_lines:
23°C, wild-type enzyme
23°C, pH 6.5, wild-type enzyme


proof_lines:
23°C, mutant enzyme P1G
23°C, pH 6.5, mutant enzyme P1G


proof_lines: wild type enzyme, with 50 mM dithiothreitol in 35 mM HEPES, pH 7.8, at 10°C


proof_lines: mutant enzyme, with 50 mM dithiothreitol in 35 mM HEPES, pH 7.8, at 10°C


proof_lines: mutant enzyme G148D, with 50 mM dithiothreitol in 35 mM HEPES, pH 7.8, at 10°C


organism: Mycoplasmoides genitalium

proof_lines: disulfide-reduced and S-carboxymethylated form of a variant of Rnase T1 with Ser54Gly and Pro55Asn


organism: Oryctolagus cuniculus

assay_conditions_summary: pH 7.4; 293.15 K; cofactor ATP

proof_lines: 20°C, pH 7.4, wild-type, presence of 1 mM MgATP


mutation_type: deletion

proof_lines: 20°C, pH 7.4, mutant DELTA208-213, presence of 1 mM MgATP


organism: Mycolicibacterium smegmatis

substrate_name: phenylenolpyruvate

assay_conditions_summary: pH 7.3; 295.15 K; buffer 10 mM Na2HPO4

proof_lines: wild type enzyme cis-CAAD, in 10 mM Na2HPO4 buffer, pH 7.3, at 22°C

proof_lines: wild type enzyme CCH2-P1A, in 10 mM Na2HPO4 buffer, pH 7.3, at 22°C

proof_lines: wild type enzyme CCH2, in 10 mM Na2HPO4 buffer, pH 7.3, at 22°C

substrate: phenylenolpyruvate


proof_lines: mutant enzyme CCH2-P1A, in 10 mM Na2HPO4 buffer, pH 7.3, at 22°C


organism: Saccharomyces cerevisiae

proof_lines: 20°C, pH 7.4, wild-type Ypa2, presence of 1 mM MgATP

proof_lines: 20°C, pH 7.4, wild-type Ypa1, presence of 1 mM MgATP


proof_lines: 20°C, pH 7.4, mutant D205G of Ypa1, presence of 1 mM MgATP


organism: Pseudomonas pavonaceae

assay_conditions_summary: pH 9


organism: Pseudomonas aeruginosa

substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

substrate: NADPH


organism: Streptomyces avermitilis

substrate_name: NADH

assay_conditions_summary: pH 6.2; 298.15 K; cofactor NADPH

proof_lines:
pH 6.2, 25°C
cosubstrate NADPH, pH 6.2, 25°C
cosubstrate NADH, pH 6.2, 25°C

substrate: NADH


organism: Acidobacteriota bacterium

substrate_name: thio-NAD+

assay_conditions_summary: pH 6.2; 325.15 K; cofactor NADPH

proof_lines:
cosubstrate NADPH, pH 6.2, 52°C
cosubstrate NADH, pH 6.2, 52°C
pH 6.2, 52°C

substrate: thio-NAD+


organism: Azotobacter vinelandii

substrate_name: thio-NADP+

assay_conditions_summary: 298.15 K; cofactor NADP+

proof_lines:
cosubstrate NADH
+ thio-NAD+
at 25°C

substrate: thio-NADP+


organism: Escherichia coli

assay_conditions_summary: pH 7.5; 308.15 K; cofactor NADPH


organism: Nocardia jiangxiensis

assay_conditions_summary: pH 5.7; 308.15 K; cofactor NADPH

proof_lines:
pH 5.7, 35°C
cosubstrate NADH, pH 5.7, 35°C
cosubstrate NADPH, pH 5.7, 35°C


organism: Bacillus subtilis


organism: Legionella pneumophila


organism: Vicia faba


enzyme_display_name: sucrose alpha-glucosidase

organism: Xanthomonas campestris pv. campestris

substrate_name: sucrose

enzyme: sucrose alpha-glucosidase

substrate: sucrose


organism: Homo sapiens

proof_lines: pH and temperature not specified in the publication


organism: Mus musculus


organism: Sus scrofa

substrate_name: phenylpyruvate

proof_lines:
keto-form, kidney
enol-form, kidney
thyroid gland

substrate: phenylpyruvate


substrate_name: PPIL1; nSKIP

proof_lines: using Trp-Phe-Tyr-pSer-Pro-Arg-4-nitroanilide as substrate, in 50 mM HEPES, 0.1 M NaCl, 5 mM NaN3, pH 7.4, at 22°C

substrate: PPIL1; nSKIP


enzyme_display_name: inulosucrase

organism: Lactobacillus johnsonii

substrate_name: Sucrose; (2,1-beta-D-Fructosyl)n

enzyme: inulosucrase

substrate: Sucrose; (2,1-beta-D-Fructosyl)n


organism: Lactobacillus reuteri


organism: Suncus murinus

substrate_name: H2O; Maltose

substrate: H2O; Maltose


organism: Xanthomonas axonopodis

proof_lines:
pH 7.5, 30°C
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=30°C, pH=7.5


enzyme_display_name: poly(3-hydroxybutyrate) depolymerase

organism: Acidovorax sp.

proof_lines:
linear 3-hydroxybutyrate oligomer
cyclic 3-hydroxybutyrate oligomer
linear 3-hydroxybutyrateoligomer

enzyme: poly(3-hydroxybutyrate) depolymerase


organism: Cupriavidus necator

proof_lines:
linear 3-hydroxybutyrate oligomer
cyclic 3-hydroxybutyrate oligomer


organism: Rhodospirillum rubrum


organism: Streptomyces sp.


organism: Ralstonia pickettii

substrate_name: 4-nitrophenyl pentanoate

proof_lines: recombinant mutant A66V/N285D/G310G, pH 7.0, 30°C

substrate: 4-nitrophenyl pentanoate


substrate_name: 4-nitrophenyl hexanoate

proof_lines: recombinant mutant T145S/S246T/T252T/S257T, pH 7.0, 30°C

substrate: 4-nitrophenyl hexanoate


substrate_name: 4-nitrophenyl acetate

proof_lines: recombinant mutant T37A/N285Y, pH 7.0, 30°C

substrate: 4-nitrophenyl acetate


proof_lines: recombinant mutant A66V/N251S/T290A, pH 7.0, 30°C


proof_lines: recombinant mutant S92S/G180D/I292F, pH 7.0, 30°C


organism: Thermobifida sp. BCC23166

substrate_name: p-nitrophenyl butyrate

substrate: p-nitrophenyl butyrate


organism: Burkholderia pickettii

substrate_name: H2O; Poly[(R)-3-hydroxybutanoate]n

substrate: H2O; Poly[(R)-3-hydroxybutanoate]n


substrate_name: H2O; Poly[(R)-3-hydroxybutanoate]n-x

substrate: H2O; Poly[(R)-3-hydroxybutanoate]n-x


organism: Lihuaxuella thermophila


substrate_name: 4-nitrophenyl butyrate

substrate: 4-nitrophenyl butyrate


proof_lines: recombinant mutant T37A/N285D, pH 7.0, 30°C


proof_lines: recombinant mutant S92S/T145S/N285Y, pH 7.0, 30°C


proof_lines: recombinant mutant G108G/Q125L/R230R/K260M/T284S/G310G, pH 7.0, 30°C


proof_lines: recombinant wild-type enzyme, pH 7.0, 30°C


proof_lines: recombinant mutant A66V/T145S/N285D, pH 7.0, 30°C


proof_lines: recombinant mutant T252T/S257T/N285D, pH 7.0, 30°C


organism: Acidovorax sp. sa1


enzyme_display_name: morphine 6-dehydrogenase

substrate_name: morphine

enzyme: morphine 6-dehydrogenase

substrate: morphine


organism: Mesocricetus auratus

assay_conditions_summary: cofactor NAD+

proof_lines: similar values of other 17beta-hydroxysteroids


organism: Oryctolagus cuniculus

substrate_name: codeine

proof_lines:
with NAD+
cosubstrate NAD+

substrate: codeine


organism: Pseudomonas putida

assay_conditions_summary: cofactor NADP+

proof_lines:
cosubstrate NADP+
cosubstrate NADPH
with NADP+


organism: Rattus norvegicus

proof_lines: cosubstrate NAD+, in the female adult rats

proof_lines: cosubstrate NAD+, in the male adult rats


enzyme_display_name: peptidyl-Lys metalloendopeptidase

organism: Armillaria mellea

substrate_name: SAQKRVS

proof_lines: recombinant wild-type enzyme, pH 7.5, temperature not specified in the publication

enzyme: peptidyl-Lys metalloendopeptidase

substrate: SAQKRVS


organism: Grifola frondosa


enzyme_display_name: UDP-sugar diphosphatase

organism: Sus scrofa

substrate_name: H2O; UDPglucose

enzyme: UDP-sugar diphosphatase

substrate: H2O; UDPglucose


organism: Cavia porcellus

substrate_name: NAD+; Morphine

proof_lines:
cosubstrate NAD+
cosubstrate NADP+
with NADP+

substrate: NAD+; Morphine


organism: Unknown

substrate_name: GDP-L-fucose

substrate: GDP-L-fucose


organism: Homo sapiens

proof_lines: pH and temperature not specified in the publication


organism: Mus musculus

substrate_name: GDP-L-Fuc

proof_lines:
pH 7.5, 37°C
Raw SKiD Env: Temp=37°C, pH=7.5

substrate: GDP-L-Fuc


organism: Methanothermobacter thermautotrophicus

assay_conditions_summary: pH 8; 338.15 K; cofactor CoA


organism: Pyrococcus sp.

substrate_name: pyruvate

assay_conditions_summary: pH 6.9; 358.15 K; cofactor CoA

substrate: pyruvate


organism: Thermococcus litoralis

substrate_name: hydroxyphenylpyruvate

proof_lines:
pH 6.9, 85°C, under argon
pH 7.0, 85°C, under argon

substrate: hydroxyphenylpyruvate


organism: Cricetulus griseus

substrate_name: factor VII EGF-1 domain

substrate: factor VII EGF-1 domain


enzyme_display_name: ferric-chelate reductase (NADH)

organism: Beta vulgaris

substrate_name: NADH; H+; Fe3+

assay_conditions_summary: cofactor NADH

enzyme: ferric-chelate reductase (NADH)

substrate: NADH; H+; Fe3+


substrate_name: Fe(III)-nitrilotriacetic acid[side 2]

proof_lines: in 25 mM MOPS, 25 mM MES, 5.4 mM KCl, 5 mM glucose, 140 mM NaCl, 1.8 mM CaCl2, 0.8 mM MgCl2, pH 7.0, at 37°C

proof_lines: in 25 mM MOPS, 25 mM MES, 5.4 mM KCl, 5 mM glucose, 140 mM NaCl, 1.8 mM CaCl2, 0.8 mM MgCl2, pH 7.0, at 22°C

substrate: Fe(III)-nitrilotriacetic acid[side 2]


substrate_name: ferricytochrome b5

proof_lines: short ferricytochrome b5 from rabbit microsomes, at pH 6.5 and 30°C

proof_lines: long ferricytochrome b5 from pig microsomes, at pH 6.5 and 30°C

proof_lines: long ferricytochrome b5 from rabbit microsomes, at pH 6.5 and 30°C

substrate: ferricytochrome b5


proof_lines: short ferricytochrome b5 from pig microsomes, at pH 6.5 and 30°C

proof_lines: long ferricytochrome b5 from rat microsomes, at pH 6.5 and 30°C


proof_lines: short ferricytochrome b5 from rat microsomes, at pH 6.5 and 30°C


enzyme_display_name: protein-histidine pros-kinase

organism: Saccharomyces cerevisiae

substrate_name: ATP

assay_conditions_summary: cofactor ATP

enzyme: protein-histidine pros-kinase

substrate: ATP


enzyme_display_name: chitin disaccharide deacetylase

organism: Pyrococcus horikoshii

substrate_name: N,N'-diacetylchitobiose

enzyme: chitin disaccharide deacetylase

substrate: N,N'-diacetylchitobiose


organism: Shewanella baltica


organism: Thermococcus kodakarensis


organism: Vibrio parahaemolyticus


proof_lines: pH 7.0, 37°C, recombinant mutant enzyme lacking the carbohydrate-binding domains


organism: Rattus norvegicus


enzyme_display_name: yapsin 1

substrate_name: Adrenocorticotropic hormone 1-39; H2O

enzyme: yapsin 1

substrate: Adrenocorticotropic hormone 1-39; H2O


organism: Archaeoglobus fulgidus


organism: uncultured archaeon


enzyme_display_name: arginine N(omega)-methyltransferase

organism: Streptomyces achromogenes subsp. streptozoticus

substrate_name: L-arginine

proof_lines: kcat is 5.9 min(-1) with L-arginine as substrate.

enzyme: arginine N(omega)-methyltransferase

substrate: L-arginine


enzyme_display_name: norajmaline N-methyltransferase

organism: Rauvolfia serpentina

substrate_name: S-adenosyl-L-methionine

proof_lines: kcat is 1.8 sec(-1) with S-adenosyl-L-methionine as substrate (PubMed:27122470). kcat is 2.1 sec(-1) with norajmaline as substrate (PubMed:27122470).

enzyme: norajmaline N-methyltransferase

substrate: S-adenosyl-L-methionine


enzyme_display_name: ajmaline N-methyltransferase

proof_lines: kcat is 0.7 sec(-1) with S-adenosyl-L-methionine as substrate (PubMed:27122470). kcat is 1 sec(-1) with norajmaline as substrate (PubMed:27122470).

enzyme: ajmaline N-methyltransferase


enzyme_display_name: piperidine N-piperoyltransferase

organism: Piper nigrum

substrate_name: piperoyl-CoA

assay_conditions_summary: cofactor CoA

proof_lines: kcat is 1.01 sec(-1) with piperoyl-CoA as substrate (PubMed:33833371). kcat is 0.47 sec(-1) with piperidine as substrate (PubMed:33833371).

enzyme: piperidine N-piperoyltransferase

substrate: piperoyl-CoA


proof_lines: kcat is 0.35 sec(-1) with piperoyl-CoA as substrate (PubMed:33833371). kcat is 0.27 sec(-1) with piperidine as substrate (PubMed:33833371).


enzyme_display_name: phloroisovalerophenone synthase

organism: Humulus lupulus

proof_lines: kcat is 0.21x10(-3) sec(-1) with 4-coumaroyl-CoA as substrate (PubMed:29760092). kcat is 0.24x10(-3) sec(-1) with malonyl-CoA as substrate (PubMed:29760092). kcat is 1.2x10(-3) sec(-1) with 4-coumaroyl-CoA as substrate (in the presence of C

enzyme: phloroisovalerophenone synthase


enzyme_display_name: sulfate adenylyltransferase (ADP)

organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

proof_lines: kcat is 93.8 sec(-1) with Ap4A as substrate.

enzyme: sulfate adenylyltransferase (ADP)


proof_lines: kcat is 81.2 sec(-1) with Ap4A as substrate.


enzyme_display_name: carbendazim hydrolysing esterase

organism: Nocardioides sp. (strain SG-4G)

substrate_name: carbendazim

proof_lines: kcat is 170 min(-1) with carbendazim as substrate.

enzyme: carbendazim hydrolysing esterase

substrate: carbendazim


enzyme_display_name: 1'-carboxy-chondrochloren decarboxylase

organism: Chondromyces crocatus

substrate_name: pre-chondrochloren B

proof_lines: kcat is 15.5 min(-1) with pre-chondrochloren B as substrate.

enzyme: 1'-carboxy-chondrochloren decarboxylase

substrate: pre-chondrochloren B


organism: Caenorhabditis elegans

substrate_name: adenosine 5'-phosphosulfate

assay_conditions_summary: pH 6.8; 303.15 K; buffer 0.1 M HEPES/KOH

proof_lines: in 0.1 M HEPES/KOH buffer (pH 6.8), 0.1 M potassium phosphate (pH 6.8), 0.1 M KH2PO4 (pH 4.2), at 30°C

substrate: adenosine 5'-phosphosulfate


assay_conditions_summary: pH 8; 303.15 K; cofactor ADP

proof_lines: 4 mM adenosine 5-phosphosulfate, 30°C, pH 8


organism: Methanopyrus kandleri

proof_lines:
1 M K2HPO4
pH 8.0, 65°C
0.3 M K2HPO4


organism: Methanosarcina barkeri


organism: Methanothermus fervidus

substrate_name: formyl-tetrahydromethanopterin

substrate: formyl-tetrahydromethanopterin


organism: Methylorubrum extorquens


enzyme_display_name: phosphohistidine phosphatase

organism: Homo sapiens

enzyme: phosphohistidine phosphatase


enzyme_display_name: 2,5-diketocamphane 1,2-monooxygenase

organism: Pseudomonas putida

substrate_name: NADH

proof_lines:
component E3
component E1
component E2

enzyme: 2,5-diketocamphane 1,2-monooxygenase

substrate: NADH


enzyme_display_name: cyanidin 3-O-galactosyltransferase

organism: Apium graveolens

substrate_name: cyanidin

enzyme: cyanidin 3-O-galactosyltransferase

substrate: cyanidin


organism: Camellia sinensis var. sinensis


organism: Daucus carota

substrate_name: peonidin

proof_lines:
pH 8.6, 30°C
cosubstrate pelargonidin, pH 8.6, 30°C
cosubstrate cyanidin, pH 8.6, 30°C

substrate: peonidin


enzyme_display_name: versatile peroxidase

organism: Pleurotus eryngii

proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=-----, pH=3.5
Raw SKiD Env: Temp=25°C, pH=3

enzyme: versatile peroxidase


enzyme_display_name: beta-glucosidase

enzyme: beta-glucosidase


organism: Neotermes koshunensis

substrate_name: cellobiose

substrate: cellobiose


organism: Oryza sativa

substrate_name: cellotriose

substrate: cellotriose


substrate_name: gentiobiose

substrate: gentiobiose


organism: Paenibacillus polymyxa


organism: Phanerodontia chrysosporium


organism: Pyrococcus furiosus


organism: uncultured microorganism

substrate_name: 4-nitrophenyl beta-D-fucopyranoside

proof_lines:
Raw SKiD Env: Temp=75°C, pH=5.5
Raw SKiD Env: Temp=60°C, pH=6

substrate: 4-nitrophenyl beta-D-fucopyranoside


enzyme_display_name: isocitrate dehydrogenase (NADP(+))

organism: Archaeoglobus fulgidus

substrate_name: NAD+

assay_conditions_summary: pH 8; 333.15 K; cofactor NADP+

proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=60°C, pH=8

enzyme: isocitrate dehydrogenase (NADP(+))

substrate: NAD+


organism: Escherichia coli

substrate_name: isocitrate

substrate: isocitrate


assay_conditions_summary: pH 6.8; 310.15 K; cofactor NADP+

proof_lines:
Raw SKiD Env: Temp=37°C, pH=6.8
Raw SKiD Env: Temp=21°C, pH=7.5
Raw SKiD Env: Temp=37°C, pH=7.2


assay_conditions_summary: pH 8; 310.15 K; cofactor NADP+

proof_lines:
Raw SKiD Env: Temp=37°C, pH=8
Raw SKiD Env: Temp=-----, pH=-----


organism: Mycobacterium tuberculosis

substrate_name: NADP+

assay_conditions_summary: pH 7.5; cofactor NADP+

substrate: NADP+


organism: Saccharomyces cerevisiae

substrate_name: NADPH

proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=-----, pH=7.5

substrate: NADPH


enzyme_display_name: sucrose synthase

organism: Nitrosospira multiformis

substrate_name: UDP

enzyme: sucrose synthase

substrate: UDP


organism: Solanum tuberosum

substrate_name: UDP-alpha-D-glucose

proof_lines: recombinant enzyme expressed in Saccharomyces cerevisiae

proof_lines: recombinant enzyme expressed in Escherichia coli

substrate: UDP-alpha-D-glucose


enzyme_display_name: anthranilate phosphoribosyltransferase

organism: Arabidopsis thaliana

substrate_name: anthranilate

enzyme: anthranilate phosphoribosyltransferase

substrate: anthranilate


proof_lines: Ki above 5 mM, mutant enzyme N215S, at pH 7.4 and 30°C


proof_lines: Ki above 5 mM, mutant enzyme N215S/P257T, at pH 7.4 and 30°C


organism: Juglans regia


substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication

substrate: Unknown


organism: Saccharolobus solfataricus


substrate_name: 5-phospho-alpha-D-ribose 1-diphosphate

proof_lines: 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A/P178A

substrate: 5-phospho-alpha-D-ribose 1-diphosphate


proof_lines: 60°C, pH 7.5, 2 mM Mg2+, mutant enzyme E224Q

proof_lines: 60°C, pH 7.5, 2 mM Mg2+,mutant enzyme E224Q


enzyme_display_name: creatine kinase

organism: Danio rerio

substrate_name: Creatine

assay_conditions_summary: 298.15 K; cofactor ATP

proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant S329A

enzyme: creatine kinase

substrate: Creatine


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant K36L


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant H267A


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant E185Q


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant A205S


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant Q46E


organism: Homo sapiens

substrate_name: N-ethylglycocyamine

substrate: N-ethylglycocyamine


substrate_name: ATP

substrate: ATP


substrate_name: cyclocreatine

substrate: cyclocreatine


organism: Acidithiobacillus caldus

proof_lines:
wild-type, immobilized, pH 8.5, 42°C
wild-type, pH 8.5, 42°C


proof_lines: mutant L637M/T640V, immobilized, pH 8.5, 42°C


assay_conditions_summary: pH 7; 310.15 K; cofactor ADP

proof_lines:
pH 7.0, 37°C
cosubstrate ADP, pH 7.0, 37°C
cosubstrate UDP, pH 7.0, 37°C


substrate_name: ADP

proof_lines:
pH 7.0, 37°C
cosubstrate UDP, pH 7.0, 37°C
cosubstrate ADP, pH 7.0, 37°C

substrate: ADP


organism: Beta vulgaris

substrate_name: sucrose

proof_lines:
pH 6.5, 35°C, SuSyI
pH 6.5, 35°C, SuSyII
pH 7.5, 35°C, SuSyI

substrate: sucrose


organism: Denitrovibrio acetiphilus


organism: Glycine max

substrate_name: ADP-alpha-D-glucose

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ADP

proof_lines: with UDP as cosubstrate, at pH 7.5 and 30°C

proof_lines: with UDP-alpha-D-glucose as cosubstrate, at pH 7.5 and 30°C

substrate: ADP-alpha-D-glucose


organism: Nitrosomonas europaea

assay_conditions_summary: pH 7; 333.15 K; cofactor ADP

proof_lines:
pH 7.0, 60°C, with ADP
pH 7.0, 60°C
pH 7.0, 60°C, with UDP-glucose


proof_lines: 60°C, pH 7.5, 2 mM Mg2+, mutant enzyme D223N

proof_lines: 60°C, pH 7.5, 2 mM Mg2+,mutant enzyme D223N


proof_lines: 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme R164A


proof_lines: 60°C, pH 7.5, 2 mM Mg2+, mutant enzyme K106Q


proof_lines: 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A


proof_lines: 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme R164A/H154A

proof_lines: 60°C, pH 7.5, 0.05 mM Mg2+,mutant enzyme R164A/H154A


proof_lines: +/-0.06, I36E/M47D double mutant, pH 7.2, 37°C, 0.01 microM subunit concentration


proof_lines: +/-0.04, wild-type, pH 7.2, 37°C, 0.01 microM subunit concentration

proof_lines: 60°C, pH 7.5, 2 mM Mg2+, wild-type enzyme

proof_lines: wild-type, in the presence of 2 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C


proof_lines: mutant F149S, in the presence of 0.05 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C


proof_lines: mutant D83G/F149S, in the presence of 2 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C

proof_lines: mutant D83G/F149S, in the presence of 0.05 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C


proof_lines: mutant D83G, in the presence of 2 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C


proof_lines: mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C


proof_lines: mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C


enzyme_display_name: procollagen-proline 4-dioxygenase

proof_lines:
isoform P4H4
isoform P4H1

enzyme: procollagen-proline 4-dioxygenase


proof_lines:
isoform P4H7
isoform P4H4


proof_lines:
isoform P4H1
MW: 10000-20000
isoform P4H7


organism: Caenorhabditis elegans


organism: Dictyostelium discoideum


substrate_name: DLDLEMLAPYIPMDDDF

proof_lines: N-terminal hydroxylation site of HIF-1alpha

substrate: DLDLEMLAPYIPMDDDF


substrate_name: DLDLEMLAPYIPMD

proof_lines: C-terminal hydroxylation site of HIF-1alpha

substrate: DLDLEMLAPYIPMD


substrate_name: DLDLEMLAPYIPMDD

substrate: DLDLEMLAPYIPMDD


proof_lines:
molecular weight 5000-10000 Da
molecular weight 20000-40000 Da


proof_lines:
type I and type II enzyme
type II enzyme
type I enzyme


proof_lines:
pH 7.0, 60°C
at pH 7.0 and 60°C


proof_lines: recombinant mutant enzyme H66P/D326A, at 25Â°C, pH not specified in the publication

proof_lines: mutant enzyme H66P/D326A, at pH 8.0 and 25°C


proof_lines: recombinant wild type enzyme, at 25Â°C, pH not specified in the publication


proof_lines: recombinant mutant enzyme H66P, at 25Â°C, pH not specified in the publication


proof_lines: recombinant mutant enzyme H66R, at 25Â°C, pH not specified in the publication


proof_lines: recombinant mutant enzyme D326E, at 25Â°C, pH not specified in the publication


proof_lines: recombinant mutant enzyme D326A, at 25Â°C, pH not specified in the publication


organism: Oryctolagus cuniculus


proof_lines: mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C


proof_lines: mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C


proof_lines: mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C


proof_lines: mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C


organism: Thermococcus kodakarensis


organism: Cryptosporidium muris

assay_conditions_summary: pH 8.5; 298.15 K; cofactor ATP

proof_lines: in form of Mg-ATP, with glycocyamine as substrate, at pH 8.5 and 25°C


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, wild-type


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant T304K


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant N146C


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant A189D


proof_lines: with substrate procollagen, isozymes P4H-I and P4H-III

proof_lines: with substrate procollagen, isozyme P4H-II


organism: Unknown


organism: Cucumis sativus

substrate_name: UDPglucose

proof_lines:
isozyme SS2
isozyme SS1
cosubstrate UDPfructose, isozyme SS2

substrate: UDPglucose


assay_conditions_summary: cofactor ADP


assay_conditions_summary: pH 8; 303.15 K; cofactor ATP

proof_lines: isoform III isolated after expression in Escherichia coli, pH 8.0, 30°C

proof_lines: isoform II isolated after expression in Escherichia coli, pH 8.0, 30°C

proof_lines: pH 7.0, ubiquitous mitochondrial isozyme


enzyme_display_name: furin

enzyme: furin


organism: Pseudomonas sp.

substrate_name: cephalosporin C

substrate: cephalosporin C


proof_lines: pH 8.5, 37°C, recombinant wild-type enzyme


proof_lines: pH 8.5, 37°C, immobilized wild-type enzyme


proof_lines:
pH 8.5, 37°C, free mutant enzyme
pH 8.5, 37°C, immobilized mutant enzyme


proof_lines: recombinant immobilized enzyme, pH 8.5, 37°C


organism: Pseudomonas sp. N176

proof_lines: pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS/L154betaY

proof_lines: pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS/F72betaR


substrate_name: glutaryl-7-aminocephalosporanic acid

proof_lines: pH 8.0, 25°C, recombinant wild-type enzyme

proof_lines: recombinant wild-type enzyme, pH and temperature not specified in the publication

substrate: glutaryl-7-aminocephalosporanic acid


proof_lines: mutant enzyme with mutation Y151F in the alpha-subunit and Q50N in the beta-subunit

proof_lines: mutant enzyme with mutation Y151F in alpha-subunit and mutation Q50N in beta-subunit


proof_lines:
wild-type enzyme
pH 7.5, 37°C, wild-type enzyme


proof_lines:
wild-type
wild-type, 25°C


enzyme_display_name: chymotrypsin

organism: Bos taurus

assay_conditions_summary: pH 7.6; 298.15 K; buffer 80 mM Tris HCl

proof_lines: presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)10, pH 7.6, 25°C

proof_lines: presence of 0.5 mM cetyltributylphosphonium bromide, pH 7.8, 27°C

proof_lines: 5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, pH 7.8, 27°C

enzyme: chymotrypsin


assay_conditions_summary: pH 8

proof_lines: pH 8, temperature not specified in the publication


proof_lines: wild-type, pH 8, temperature not specified in the publication


proof_lines: mutant S242T, pH 8, temperature not specified in the publication


proof_lines: mutant D236R, pH 8, temperature not specified in the publication


organism: Penaeus californiensis


enzyme_display_name: cathepsin L

organism: Homo sapiens

proof_lines: wild-type, pH 5.5, temperature not specified in the publication

proof_lines: wild-type, substrate: benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide, production rate of 7-amino-4-methylcoumarin measured, excitation and emission wavelengths of 370 and 460 nm, pseudo first order reaction conditions (inhibitor concent

enzyme: cathepsin L


proof_lines:
mutant enzyme T223V/DELTAE286-E289
mutant enzyme T223V/DELTAE286-N293


proof_lines: G139R mutant, substrate: benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide, production rate of 7-amino-4-methylcoumarin measured, excitation and emission wavelengths of 370 and 460 nm, pseudo first order reaction conditions (inhibitor conc


proof_lines: pH and temperature not specified in the publication

proof_lines: 25°C, pH not specified in the publication


proof_lines: mutant L69Y/M161L/G164A/A214L, pH 5.5, temperature not specified in the publication


organism: Leishmania mexicana


organism: Mus musculus

proof_lines: substrate: benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide, production rate of 7-amino-4-methylcoumarin measured, excitation and emission wavelengths of 370 and 460 nm, pseudo first order reaction conditions (inhibitor concentration at l


organism: Trichinella spiralis


proof_lines: pH 7.5, 37°C, mutant enzyme M271V/Q291K/T374S


organism: Periplaneta americana


organism: Rattus norvegicus

proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant V99M/M192R/G218R/V219E/S221T/T224/W/A226G, pH not specified in the publication, temperature not specified in the publication


organism: Severe acute respiratory syndrome coronavirus 2

proof_lines: native enzyme expressed by commercial vector, pH 7, 37°C

proof_lines: engineered enzyme expressed by modified vector, pH 7, 37°C


organism: Spodoptera frugiperda


enzyme_display_name: tissue kallikrein

enzyme: tissue kallikrein


substrate_name: Abz-FRSSR-Q-EDDnp

substrate: Abz-FRSSR-Q-EDDnp


proof_lines: pH 8.0, temperature not specified in the publication


proof_lines: pH 7.0, temperature not specified in the publication


organism: Trichoplusia ni


organism: Clonorchis sinensis


enzyme_display_name: acetoacetyl-CoA hydrolase

substrate_name: decanoyl-CoA

enzyme: acetoacetyl-CoA hydrolase

substrate: decanoyl-CoA


enzyme_display_name: hydroxymethylglutaryl-CoA hydrolase

enzyme: hydroxymethylglutaryl-CoA hydrolase


enzyme_display_name: 2,3-bisphosphoglycerate 3-phosphatase

organism: Gallus gallus

substrate_name: inositol hexakisphosphate

enzyme: 2,3-bisphosphoglycerate 3-phosphatase

substrate: inositol hexakisphosphate


organism: Arabidopsis thaliana

substrate_name: ABA-beta-D-glucopyranosyl ester

substrate: ABA-beta-D-glucopyranosyl ester


enzyme_display_name: spermosin

organism: Halocynthia roretzi

substrate_name: Boc-Val-Pro-Arg-MCA

enzyme: spermosin

substrate: Boc-Val-Pro-Arg-MCA


organism: Enterobacter agglomerans


organism: Drosophila melanogaster

substrate_name: L-Dopa

substrate: L-Dopa


enzyme_display_name: phenylacetaldehyde synthase

organism: Petunia hybrida

substrate_name: L-phenylalanine

enzyme: phenylacetaldehyde synthase

substrate: L-phenylalanine


organism: Nocardioides sp. (strain KP7)


enzyme_display_name: cyanide hydratase

organism: Gibberella baccata

substrate_name: cyanide

enzyme: cyanide hydratase

substrate: cyanide


organism: Microdochium sorghi

substrate_name: cyanide (at pH 8 and 25 degrees Celsium)


enzyme_display_name: peptidylprolyl isomerase

organism: Homo sapiens

enzyme: peptidylprolyl isomerase


organism: Pseudomonas sp.

substrate_name: glutaryl-7-amino cephalosporanic acid

substrate: glutaryl-7-amino cephalosporanic acid


proof_lines: pH 8.0, 37°C, mutant enzyme S22P/T394P/D416Y/H417Y


proof_lines: pH 8.0, 37°C, mutant enzyme I44V/E49stop/D416Y/H417Y


proof_lines: mutant enzyme H296S/H309S, at pH 8.0 and 25°C


proof_lines: pH 8.0, 37°C, mutant enzyme A215Y/H296S/H309S


enzyme_display_name: tyrosinase

organism: Camellia sinensis

substrate_name: catechol

enzyme: tyrosinase

substrate: catechol


enzyme_display_name: carboxylesterase

organism: Rattus norvegicus

substrate_name: retinyl palmitate

enzyme: carboxylesterase

substrate: retinyl palmitate


organism: Pseudomonas sp. SY-77-1


substrate_name: adipyl-7-aminocephalosporanic acid

substrate: adipyl-7-aminocephalosporanic acid


enzyme_display_name: glutathione transferase

organism: Anopheles dirus

assay_conditions_summary: pH 6.5; buffer pH 6

proof_lines: mutant enzyme Y215E, in 0.1 M phosphate buffer pH 6.5

enzyme: glutathione transferase


proof_lines: mutant enzyme R214A, in 0.1 M phosphate buffer pH 6.5


substrate_name: Ethacrynic acid

proof_lines: mutant enzyme Y215A, in 0.1 M phosphate buffer pH 6.5

substrate: Ethacrynic acid


proof_lines: wild type enzyme, in 0.1 M phosphate buffer pH 6.5


proof_lines: mutant enzyme T174A, in 0.1 M phosphate buffer pH 6.5


proof_lines: mutant enzyme F212E, in 0.1 M phosphate buffer pH 6.5


proof_lines: mutant enzyme F212A, in 0.1 M phosphate buffer pH 6.5


proof_lines: mutant enzyme Q112A, in 0.1 M phosphate buffer pH 6.5


organism: Phanerodontia chrysosporium

substrate_name: 4-nitrophenyl butyrate

proof_lines: recombinant mutant S22A, pH 6.5, temperature not specified in the publication

substrate: 4-nitrophenyl butyrate


substrate_name: phenethyl isothiocyanate

substrate: phenethyl isothiocyanate


organism: Pinus tabuliformis


organism: Populus trichocarpa

substrate_name: sulforaphane

proof_lines: isoform GSTU45, pH not specified in the publication, at 30°C

substrate: sulforaphane


proof_lines:
wild-type enzyme
pH 7.5, 37°C, wild-type enzyme


enzyme_display_name: cellobiose dehydrogenase (acceptor)

organism: Gelatoporia subvermispora

substrate_name: cellobiose

proof_lines: 2,6-dichloroindophenol as electron acceptor

enzyme: cellobiose dehydrogenase (acceptor)

substrate: cellobiose


organism: Mycothermus thermophilus


substrate_name: lactose

substrate: lactose


substrate_name: 2,6-dichlorophenol indophenol

proof_lines: pH 4.5, reaction with cellobiose, wild-type enzyme

proof_lines: pH 4.5, electron acceptor: cytochrome c, wild-type enzyme

substrate: 2,6-dichlorophenol indophenol


proof_lines: pH 4.5, electron acceptor: cytochrome c, mutant enzyme M65H

proof_lines: pH 4.5, reaction with cellobiose, mutant enzyme M65H

proof_lines: pH 4.5, electron acceptor: 2,6-dichlorophenol indophenol, mutant enzyme M65H


substrate_name: cytochrome c

proof_lines: mutant F166Y, cellobiose oxidation with electron acceptors

substrate: cytochrome c


assay_conditions_summary: pH 4; 303.15 K; cofactor FAD

proof_lines: reduction rate increases with increasing concententrations of the electron acceptors methyl-1,4-benzoquinone or Fe3+

proof_lines: pH 4.5, 30°C, acceptor: 2,6-dichlorophenolindophenol


enzyme_display_name: laccase

organism: Escherichia coli

proof_lines: mutant M510L, pH 5.5, temperature not specified in the publication

enzyme: laccase


enzyme_display_name: formate dehydrogenase

organism: [Candida] boidinii

proof_lines: at pH 7.4, temperature not specified in the publication

proof_lines: in the absence of metal ion, at pH 7.4 and 25°C

proof_lines: in the presence of 0.003 mM W6+, at pH 7.4 and 25°C

enzyme: formate dehydrogenase


organism: Clostridium ljungdahlii


organism: Cupriavidus necator

substrate_name: NADH

proof_lines: pH and temperature not specified in the publication

substrate: NADH


organism: Moraxella sp.

substrate_name: formate

proof_lines: in the presence of 0.005 mM Na+, at pH 5.5 and 25°C

proof_lines: in the absence of metal ion, at pH 5.5 and 25°C

proof_lines: in 0.1 M potassium phosphate buffer pH 7.0, at 30°C

substrate: formate


enzyme_display_name: alcohol dehydrogenase

organism: Saccharomyces cerevisiae

substrate_name: ethanol

enzyme: alcohol dehydrogenase

substrate: ethanol


organism: Agroathelia rolfsii

substrate_name: maltose

substrate: maltose


organism: Amesia atrobrunnea


organism: Cerrena unicolor

proof_lines: with ferricytochrome c as cosubstrate, at pH 4.5 and 30°C

proof_lines: with 2,6-dichlorophenolindophenol as cosubstrate, at pH 4.5 and 30°C


organism: Coniophora puteana


proof_lines: mutant enzyme F216A, in 0.1 M phosphate buffer pH 6.5


proof_lines: mutant enzyme Y215H, in 0.1 M phosphate buffer pH 6.5


proof_lines: mutant enzyme F212H, in 0.1 M phosphate buffer pH 6.5


proof_lines: mutant enzyme Y215S, in 0.1 M phosphate buffer pH 6.5


organism: Pinus brutia


organism: Plasmodium falciparum

substrate_name: glutathione

substrate: glutathione


organism: Plasmodium vivax


organism: Plasmodium yoelii


organism: Populus trichocarpa

substrate_name: allyl-isothiocyanate

proof_lines: isoform GSTU16, pH not specified in the publication, at 30°C

substrate: allyl-isothiocyanate


organism: Salix babylonica


enzyme_display_name: feruloyl esterase

organism: Actinomyces sp. oral taxon 448

substrate_name: methyl caffeate

enzyme: feruloyl esterase

substrate: methyl caffeate


organism: Anaeromyces mucronatus

substrate_name: ethyl ferulate

substrate: ethyl ferulate


organism: Aspergillus awamori

substrate_name: alpha-naphthylbutyrate

substrate: alpha-naphthylbutyrate


substrate_name: alpha-naphthylcaprylate

substrate: alpha-naphthylcaprylate


organism: Aspergillus nidulans

substrate_name: methyl ferulate

proof_lines: recombinant His-tagged enzyme, pH 7.0, 37°C

substrate: methyl ferulate


organism: Aspergillus niger

proof_lines:
isoenzyme FAEB
FAEB


organism: Thermothelomyces myriococcoides


organism: Trametes sanguinea

proof_lines:
pH 4.5, 30°C, without polysaccharide
at pH 4.5 and 30°C


organism: Trametes versicolor


enzyme_display_name: glucuronosyltransferase

organism: Homo sapiens

substrate_name: propofol

proof_lines: UGT1A9/UGT2B7(mutant H35A)-CFP heterodimer, pH 7.4, 37°C

enzyme: glucuronosyltransferase

substrate: propofol


enzyme_display_name: purine-nucleoside phosphorylase

organism: Helicobacter pylori

substrate_name: guanosine

proof_lines:
pH 7.0, 25°C
pH 7.0, 25°C, with guanosine
pH 7.0, 25°C, with 7-methylguanosine

enzyme: purine-nucleoside phosphorylase

substrate: guanosine


organism: Anopheles dirus

proof_lines: mutant enzyme Y215F, in 0.1 M phosphate buffer pH 6.5


proof_lines: mutant enzyme F212Y, in 0.1 M phosphate buffer pH 6.5


substrate_name: Ethacrynic acid

proof_lines: mutant enzyme F212L, in 0.1 M phosphate buffer pH 6.5

substrate: Ethacrynic acid


proof_lines: mutant enzyme F212W, in 0.1 M phosphate buffer pH 6.5


organism: Physcomitrium patens

substrate_name: lactose

assay_conditions_summary: pH 6; cofactor FAD

proof_lines: at pH 6.0, temperature not specified in the publication

substrate: lactose


organism: Thermothelomyces fergusii


assay_conditions_summary: pH 6; 303.15 K; buffer 100 mM McIlvaine

proof_lines: mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C


proof_lines: mutant enzyme 700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C


proof_lines: recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C


organism: Neurospora crassa


organism: Parageobacillus thermoglucosidasius

substrate_name: 4-nitrophenyl laurate

substrate: 4-nitrophenyl laurate


organism: Piromyces sp. 'equi'


organism: Pleurotus eryngii


organism: Pleurotus sapidus


organism: Pseudoalteromonas haloplanktis

substrate_name: methylumbelliferyl acetate

substrate: methylumbelliferyl acetate


organism: Rhizoctonia solani


organism: Schizophyllum commune

substrate_name: methyl 4-hydroxybenzoate

substrate: methyl 4-hydroxybenzoate


organism: Streptomyces cinnamoneus

proof_lines: pH not specified in the publication, 50°C


organism: Streptomyces olivochromogenes


organism: Streptomyces sp. TH-2

proof_lines: mutant 18PG, pH not specified in the publication, 50°C


organism: Aspergillus niger

substrate_name: ferulate

substrate: ferulate


proof_lines:
mutant D93G/S187F, pH 6.4, 40°C
pH 6.4, 40°C, mutant D93G/S187F


proof_lines:
pH 5.0, 55°C
pH 6.0, 40°C
isoenzyme FAEA


organism: Aspergillus oryzae


substrate_name: methyl 4-coumarate

assay_conditions_summary: pH 6

substrate: methyl 4-coumarate


substrate_name: alpha-naphthyl propionate

substrate: alpha-naphthyl propionate


substrate_name: methyl p-coumarate

substrate: methyl p-coumarate


organism: Aspergillus terreus

substrate_name: ferulic acid methyl ester

substrate: ferulic acid methyl ester


proof_lines: wild-type, pH not specified in the publication, 50°C


organism: Talaromyces funiculosus


organism: Talaromyces pinophilus

substrate_name: 4-nitrophenyl acetate

substrate: 4-nitrophenyl acetate


organism: Talaromyces stipitatus


organism: Thermobacillus xylanilyticus

substrate_name: ethyl ferulate

substrate: ethyl ferulate


organism: Thermochaetoides dissita

substrate_name: 4-nitrophenyl butanoate

substrate: 4-nitrophenyl butanoate


organism: Thermothelomyces thermophilus


organism: Thermothielavioides terrestris


organism: uncultured bacterium


substrate_name: 4-nitrophenyl ferulate

substrate: 4-nitrophenyl ferulate


enzyme_display_name: beta-glucosidase

organism: Trichoderma viride

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

enzyme: beta-glucosidase

substrate: 4-nitrophenyl beta-D-glucopyranoside


enzyme_display_name: trypsin

organism: Bos taurus

substrate_name: Unknown

enzyme: trypsin

substrate: Unknown


organism: Unknown


organism: Fusarium oxysporum

proof_lines:
pH 6.0, 45°C
45°C, pH 6.0, isoenzyme FAE-I
pH 7.0, 45°C


organism: Neocallimastix sp.

proof_lines:
40°C, pH 6.2, isoenzyme FAE-I
40°C, pH 7.0, isoenzyme FAE-II
FAE-II


organism: Streptomyces cinnamoneus

proof_lines:
isozyme R43, pH 7.0, 50°C
isozyme R18, pH 7.0, 50°C


substrate_name: methyl ferulate

substrate: methyl ferulate


organism: Homo sapiens

substrate_name: enhanced green fluorescent protein-T1

assay_conditions_summary: pH 7.4; 310.15 K; buffer 10 mM phosphate

proof_lines: in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C

proof_lines: pH 8.0, 37°C, uncleaved enzyme, nonsulfated

proof_lines: pH 8.0, 37°C, uncleaved enzyme, sulfated

substrate: enhanced green fluorescent protein-T1


organism: Butyrivibrio proteoclasticus

substrate_name: 4-nitrophenyl butyrate

proof_lines: pH and temperature not specified in the publication

substrate: 4-nitrophenyl butyrate


substrate_name: methyl sinapate

substrate: methyl sinapate


organism: Lentilactobacillus buchneri


organism: Limosilactobacillus fermentum


enzyme_display_name: alcohol dehydrogenase (NADP+)

organism: Thermococcus sp.

substrate_name: NADP+

assay_conditions_summary: pH 8.8; buffer 100 mM EPPS; cofactor NADP+

proof_lines: recombinant enzyme, with 90 mM ethanol as cosubstrate, in 100 mM EPPS buffer (pH 8.8)

proof_lines: recombinant enzyme, with 0.4 mM NADP+ as cosubstrate, in 100 mM EPPS buffer (pH 8.8)

proof_lines: native enzyme, with 90 mM ethanol as cosubstrate, in 100 mM EPPS buffer (pH 8.8)

enzyme: alcohol dehydrogenase (NADP+)

substrate: NADP+


organism: Aspergillus awamori


organism: Aspergillus nidulans


organism: Aspergillus niger


organism: Aspergillus usamii

substrate_name: ferulic acid 4-nitrophenyl ester

substrate: ferulic acid 4-nitrophenyl ester


organism: Bacillus amyloliquefaciens


organism: Burkholderia pyrrocinia


organism: Thermococcus guaymasensis

substrate_name: acetoin

assay_conditions_summary: pH 7.5; 353.15 K; cofactor NADP+

proof_lines:
cosubstrate: NADPH, pH 7.5, 80°C
cosubstrate: NADP+, pH 7.5, 80°C
cosubstrate: 2-butanone, pH 7.5, 80°C

substrate: acetoin


enzyme_display_name: procollagen-proline 4-dioxygenase

organism: Chlamydomonas reinhardtii

substrate_name: poly(L-proline)

enzyme: procollagen-proline 4-dioxygenase

substrate: poly(L-proline)


proof_lines:
mutant S78T/S87L
mutants S78T/S87L


organism: Geobacillus thermodenitrificans

substrate_name: NAD+

proof_lines:
cosubstrate octan-1-ol, pH 8, 60°C
pH 8, 60°C
cosubstrate octanal, pH 8, 60°C

substrate: NAD+


substrate_name: p-nitrobenzaldehyde

proof_lines:
mutant R311A
aldehyde reductase, mutant R311A
mutant enzyme R311A, at pH 7.0 and 25°C

substrate: p-nitrobenzaldehyde


substrate_name: p-Carboxybenzaldehyde

proof_lines:
aldehyde reductase, mutant R308A
mutant enzyme R308A, at pH 7.0 and 25°C

substrate: p-Carboxybenzaldehyde


organism: Kluyveromyces lactis

substrate_name: NADH

proof_lines: wild type enzyme, at 25°C, pH not specified in the publication

substrate: NADH


proof_lines: mutant enzyme A274F, at 25°C, pH not specified in the publication


substrate_name: NADPH

proof_lines: mutant enzyme G225A/A274F, at 25°C, pH not specified in the publication

substrate: NADPH


substrate_name: acetaldehyde

proof_lines: mutant enzyme G225A, at 25°C, pH not specified in the publication

substrate: acetaldehyde


organism: Mycobacterium sp.


organism: Pectobacterium atrosepticum

substrate_name: Butanal

assay_conditions_summary: pH 7

proof_lines: pH 7.0, temperature not specified in the publication

substrate: Butanal


organism: Scheffersomyces stipitis


proof_lines: substrate 5-hydroxymethylfurfural, pH 7.2, 30°C


organism: Vanderwaltozyma polyspora

assay_conditions_summary: pH 9.5; 303.15 K; cofactor NADP+

proof_lines: pH 9.5, 30°C, recombinant mutant S196A/F197W, with 2-hydroxytetrahydrofuran


proof_lines: pH 9.5, 30°C, recombinant mutant V127C, with 2-hydroxytetrahydrofuran


proof_lines: pH 9.5, 30°C, recombinant mutant V84I/Y127M, with 2-hydroxytetrahydrofuran


proof_lines: pH 9.5, 30°C, recombinant mutant F197W, with 2-hydroxytetrahydrofuran


proof_lines: pH 9.5, 30°C, recombinant mutant Y127C/S196A, with 2-hydroxytetrahydrofuran


proof_lines: pH 9.5, 30°C, recombinant mutant V127M, with 2-hydroxytetrahydrofuran


proof_lines: pH 9.5, 30°C, recombinant wild-type enzyme, with 2-hydroxytetrahydrofuran


proof_lines: pH 9.5, 30°C, recombinant mutant S196A, with 2-hydroxytetrahydrofuran


enzyme_display_name: ribulose-bisphosphate carboxylase

organism: Thermosynechococcus vestitus

proof_lines: mutation P415A in subunit RbcL, plus mutation Y36N/R51H in subunit RbcS, pH 8.1, 25°C

enzyme: ribulose-bisphosphate carboxylase


proof_lines: mutation A398T/P415A in subunit RbcL, pH 8.1, 25°C


enzyme_display_name: monoamine oxidase

organism: Unknown

substrate_name: kynuramine

enzyme: monoamine oxidase

substrate: kynuramine


organism: Homo sapiens

proof_lines: pH 7.5, MAO-A incorporated into phospholipid nanodisks


organism: Rattus norvegicus

substrate_name: Phenylethylamine

proof_lines:
pH 7.5, 25°C, MAO-A
monoamine oxidase A
MAO-A

substrate: Phenylethylamine


substrate_name: benzylamine

proof_lines:
pH 7.5
MAO-B
above, MAO-B

substrate: benzylamine


enzyme_display_name: creatine kinase

substrate_name: Creatine

assay_conditions_summary: 298.15 K; cofactor ATP

proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant L36K

enzyme: creatine kinase

substrate: Creatine


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant A267H


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant D189A


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant Q185E


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant S205A


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant C146N


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant A329S


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant E46Q


proof_lines:
pH 7.8, 30°C, enzyme variant D9
pH 7.8, 30°C, variant D11


proof_lines: mutant enzyme W230I/T354S/W430R, pH and temperature not specified in the publication


proof_lines: mutant enzyme W230I/W430R, pH and temperature not specified in the publication


proof_lines: mutant enzyme W230R/W430C/C214L, pH and temperature not specified in the publication


proof_lines: mutant enzyme W230I/T354S/W430R/M242R/Y365V, pH and temperature not specified in the publication


substrate_name: serotonin

substrate: serotonin


substrate_name: p-nitrophenylethylamine

substrate: p-nitrophenylethylamine


organism: Pseudomonas sp.


substrate_name: glutaryl-7-aminocephalosporanic acid

substrate: glutaryl-7-aminocephalosporanic acid


enzyme_display_name: acetylcholinesterase

organism: Mus musculus

substrate_name: Acetylcholine

enzyme: acetylcholinesterase

substrate: Acetylcholine


organism: Bos taurus

substrate_name: Unknown

substrate: Unknown


proof_lines:
pH 7.4, 37°C
pH 7.4, 38°C
in presence of 0.007 mM KCN


proof_lines:
monoamine oxidase B
isoform MAO B, at pH 7.4 and 37°C
pH 7.4, 30°C, reductive half-reaction


proof_lines:
pH 7.4, 37°C, enzyme from brain
pH 7.4, 37°C, enzyme from liver
inhibition of MAO-B


proof_lines: pH 7.0, 22°C, recombinant wild-type enzyme


organism: [Candida] boidinii

substrate_name: NADP+

assay_conditions_summary: pH 7.5; buffer 0.1 M potassium phosphate; cofactor NADP+

proof_lines: mutant enzyme D195Q/Y196P, in 0.1 M potassium phosphate buffer (pH 7.5)

substrate: NADP+


proof_lines: mutant enzyme D195Q, in 0.1 M potassium phosphate buffer (pH 7.5)


proof_lines: mutant enzyme D195N, in 0.1 M potassium phosphate buffer (pH 7.5)


substrate_name: NAD+

proof_lines: mutant enzyme D195Q/Y196S, in 0.1 M potassium phosphate buffer (pH 7.5)

substrate: NAD+


organism: Oryctolagus cuniculus

substrate_name: ATP

assay_conditions_summary: pH 9; 303.15 K; cofactor ATP

substrate: ATP


assay_conditions_summary: cofactor ATP

proof_lines:
ATP in form of MgATP2-
fusion protein CK-AK
oxidized form of creatine kinase


proof_lines:
wild-type enzyme
wild-type


substrate_name: poly(L-proline)

substrate: poly(L-proline)


organism: Cimex lectularius

proof_lines: 25°C, pH not specified in the publication


substrate_name: heroin

proof_lines: recombinant enzyme, pH and temperature not specified in the publication

substrate: heroin


substrate_name: acetylthiocholine

substrate: acetylthiocholine


organism: Thermoanaerobacter ethanolicus


assay_conditions_summary: 303.15 K; cofactor FAD


organism: Ctenocephalides felis

substrate_name: propionylthiocholine

proof_lines: isoform AChE2, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C

substrate: propionylthiocholine


proof_lines: isoform AChE1, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C


organism: Danio rerio

proof_lines: muscle isoform, pH 8, temperature not specified in the publication

proof_lines: brain isoform, pH 8, temperature not specified in the publication


organism: Lucilia cuprina

proof_lines: native enzyme from head extract, at 22°C in 10 mM Na2HPO4/NaH2PO4, pH 7.4

proof_lines: recombinant enzyme, at 22°C in 10 mM Na2HPO4/NaH2PO4, pH 7.4


organism: Pardosa pseudoannulata

proof_lines: pH 7.0, temperature not specified in the publication


organism: Stomoxys calcitrans

assay_conditions_summary: pH 7.5; 298.15 K; buffer condition do not establish a strict measurement record

proof_lines: in potassium phosphate buffer (100 mM, pH 7.5), at 25°C


organism: Pseudomonas sp. 130

proof_lines: pH 8.0, 30°C, beta-subunitmutant enzyme H23D


proof_lines: pH 8.0, 30°C, beta-subunit mutant enzyme H23Q

proof_lines: pH 8.0, 30°C, beta-subunitmutant enzyme H23Q


proof_lines:
pH 8.0, 30°C, wild-type enzyme
pH 8.0, 37°C, wild-type enzyme


proof_lines: pH 8.0, 30°C, beta-subunitmutant enzyme E455Q


proof_lines: pH 8.0, 30°C, beta-subunitmutant enzyme E455D


proof_lines: pH 8.0, 30°C, beta-subunitmutant enzyme E455L

proof_lines: pH 8.0, 30°C, beta-subunit mutant enzyme E455L


organism: Aphis citricidus


proof_lines: pH 7.5, temperature not specified in the publication


assay_conditions_summary: pH 8

proof_lines: pH 8.0, temperature not specified in the publication


organism: Chlamydomonas reinhardtii

substrate_name: poly(L-Pro)

substrate: poly(L-Pro)


organism: Homo sapiens


proof_lines:
pH 8.0, 27°C, wild-type
wild-type enzyme


organism: Glycine max

assay_conditions_summary: pH 7; buffer 0.1 M sodium phosphate; cofactor NAD+

proof_lines: mutant enzyme F290Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme F290T, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme F290Q, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme F290E, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


substrate_name: p-Carboxybenzaldehyde

proof_lines:
aldehyde reductase, mutant V299C
mutant enzyme V299C, at pH 7.0 and 25°C

substrate: p-Carboxybenzaldehyde


substrate_name: Succinic semialdehyde

proof_lines:
aldehyde reductase, mutant I298A
mutant enzyme I298A, at pH 7.0 and 25°C

substrate: Succinic semialdehyde


proof_lines:
aldehyde reductase, mutant I298C
mutant enzyme I298C, at pH 7.0 and 25°C


proof_lines:
aldehyde reductase, mutant H112Q
mutant enzyme H112Q, at pH 7.0 and 25°C


substrate_name: p-nitrobenzaldehyde

proof_lines:
wild-type aldehyde reductase
wild type enzyme, at pH 7.0 and 25°C

substrate: p-nitrobenzaldehyde


organism: Thermoanaerobacter brockii

substrate_name: Cyclopentanol

substrate: Cyclopentanol


proof_lines:
r-TBADH, wild-type, mutant D150C-TBADH
r-TBADH, wild-type


organism: Musca domestica

substrate_name: acetylthiocholine iodide

substrate: acetylthiocholine iodide


organism: Heterorhabditis bacteriophora

proof_lines:
pH 8.5, 35°C, isozyme AChEA
pH 8.5, 35°C, isozyme AChEB
pH 7.5, 37°C, isozyme AChE1A


substrate_name: Phenylethylamine

substrate: Phenylethylamine


substrate_name: serotonin

substrate: serotonin


organism: Mus musculus


proof_lines:
wild-type enzyme
wild-type
pH 7.0, 25°C, wild-type


organism: Cyprinus carpio

substrate_name: butyrylthiocholine iodide

proof_lines:
pH 7.6, 40°C, recombinant enzyme
pH 7.6, 40°C, native enzyme

substrate: butyrylthiocholine iodide


organism: Electrophorus electricus

substrate_name: Unknown

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: Unknown


organism: Schistosoma bovis


organism: Schistosoma haematobium


organism: Schistosoma mansoni


organism: Tetronarce californica


organism: Rattus norvegicus


proof_lines:
pH 7.0, 25°C, mutant Y337A
pH 8, 25°C, Y337A mutant


substrate_name: tryptamine

substrate: tryptamine


substrate_name: tyramine

substrate: tyramine


organism: Oryctolagus cuniculus

substrate_name: ATP

substrate: ATP


proof_lines: pH 9.0, 30°C, recombinant wild-type enzyme

proof_lines: wild-type containing both oxidized and reduced form


organism: Drosophila melanogaster


enzyme_display_name: hypoxanthine phosphoribosyltransferase

organism: Plasmodium falciparum

substrate_name: hypoxanthine

assay_conditions_summary: pH 7.4; 296.15 K; buffer modifiers but no exact primary kinetic table/value

proof_lines: activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C

proof_lines: activated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C

proof_lines: activated enzyme, with hypoxanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C

enzyme: hypoxanthine phosphoribosyltransferase

substrate: hypoxanthine


organism: Thermus thermophilus

substrate_name: guanine

substrate: guanine


organism: Brevibacillus laterosporus

substrate_name: glutaryl-7-aminocephalosporanic acid

substrate: glutaryl-7-aminocephalosporanic acid


organism: Pseudomonas sp.


assay_conditions_summary: pH 8.5; 310.15 K; buffer 100 mM Tris-HCl

proof_lines: wild-type enzyme and deletion mutant D4(212-ADLA-215), pH 8.5, 37°C


organism: Caldanaerobacter subterraneus subsp. tengcongensis


organism: Homo sapiens

proof_lines: pH 7.4, 22°C, recombinant mutant F36L, with hypoxanthine

proof_lines: pH 7.4, 22°C, recombinant mutant F36L, with xanthine


proof_lines: pH 7.4, 22°C, recombinant mutant F36K, with guanine

proof_lines: pH 7.4, 22°C, recombinant mutant F36K, with hypoxanthine


proof_lines: pH 7.4, 22°C, recombinant mutant F36G, with hypoxanthine

proof_lines: pH 7.4, 22°C, recombinant mutant F36G, with guanine


substrate_name: xanthine

proof_lines: pH 7.4, 22°C, recombinant wild-type enzyme, with guanine

substrate: xanthine


proof_lines: pH 7.4, 28°C, recombinant chimeric mutant enzyme, with substrate guanine

proof_lines: pH 7.4, 28°C, recombinant chimeric mutant enzyme with substrate hypoxanthine


organism: Mycobacterium tuberculosis

proof_lines: with hypoxanthine as cosubstrate, at pH 7.5 and 25°C

proof_lines: with guanine as cosubstrate, at pH 7.5 and 25°C


substrate_name: 5-phospho-alpha-D-ribose 1-diphosphate

proof_lines: pH 7.4, 37°C, recombinant nonactivated wild-type enzyme

proof_lines: pH 7.4, 37°C, recombinant IMP-activated wild-type enzyme

substrate: 5-phospho-alpha-D-ribose 1-diphosphate


proof_lines: pH 7.4, 37°C, recombinant nonactivated mutant W181Y

proof_lines: pH 7.4, 37°C, recombinant IMP-activated mutant W181Y


proof_lines: pH 7.4, 37°C, recombinant nonactivated mutant W181S

proof_lines: pH 7.4, 37°C, recombinant IMP-activated mutant W181S


proof_lines: pH 7.4, 37°C, recombinant IMP-activated mutant W181F

proof_lines: pH 7.4, 37°C, recombinant nonactivated mutant W181F


substrate_name: IMP

substrate: IMP


substrate_name: cephalosporin C

assay_conditions_summary: pH 8; buffer 100 mM Tris-HCl

proof_lines: mutant enzyme H309V, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication

substrate: cephalosporin C


proof_lines: mutant enzyme P295A, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication


proof_lines: mutant enzyme L677F, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication


proof_lines: mutant enzyme A675G, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication


organism: Trypanosoma brucei brucei

proof_lines: recombinant wild-type enzyme, pH 7.4, 25°C


organism: [Candida] boidinii

substrate_name: NADP+

proof_lines: mutant enzyme D195Q/Y196H, in 0.1 M potassium phosphate buffer (pH 7.5)

substrate: NADP+


proof_lines: mutant enzyme D195A, in 0.1 M potassium phosphate buffer (pH 7.5)


substrate_name: NAD+

proof_lines: mutant enzyme D195S, in 0.1 M potassium phosphate buffer (pH 7.5)

substrate: NAD+


proof_lines: enzyme tetramer containing the H501S mutant alpha subunit


proof_lines: wild-type enzyme tetramer and enzyme tetramer containing the H501S mutant alpha subunit


proof_lines: enzyme tetramer containing the histidine 501 to serine mutant alpha subunit


organism: Escherichia coli

proof_lines: 0.1 M Tris-HCl, 12 mM MgCl2, pH 7.4, 25°C

proof_lines: pH and temperature not specified in the publication


proof_lines:
at pH 7.4 and 37°C
at pH 7.4 and 25°C
above, pH 7.4, 25°C


organism: Legionella pneumophila

proof_lines: with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C


proof_lines: with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C


organism: Leishmania tarentolae

substrate_name: 5-phosphoribosyl 1-diphosphate

proof_lines: recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate

substrate: 5-phosphoribosyl 1-diphosphate


proof_lines: pH not specified in the publication, 37°C


proof_lines: pH 7.4, temperature not specified in the publication


organism: Schistosoma mansoni

proof_lines: pH 7.5, 37°C, recombinant isozyme HGPRT-1


enzyme_display_name: alpha-glucosidase

organism: Thermotoga maritima

substrate_name: 4-nitrophenyl alpha-D-galactopyranoside

enzyme: alpha-glucosidase

substrate: 4-nitrophenyl alpha-D-galactopyranoside


organism: Thermochaetoides thermophila

proof_lines: mutant enzyme G93H/I94Y/R259C, at pH 8.0, temperature not specified in the publication

proof_lines: mutant enzyme G93H/I94Y/R259C, at pH 7.0 and 25°C


proof_lines: mutant enzyme G193H/I94Y, at pH 8.0, temperature not specified in the publication


proof_lines: mutant enzyme G93H/I94R, at pH 8.0, temperature not specified in the publication

proof_lines: mutant enzyme G93H/I94R, at pH 7.0 and 25°C


proof_lines: wild type enzyme, with HCO3- as cosubstrate, at pH 5.0, temperature not specified in the publication


proof_lines: mutant enzyme N256D, at pH 8.0, temperature not specified in the publication


proof_lines: mutant enzyme N120C, at pH 7.0, temperature not specified in the publication


organism: Sulfolobus acidocaldarius

substrate_name: 4-nitrophenyl alpha-D-glucopyranoside

substrate: 4-nitrophenyl alpha-D-glucopyranoside


organism: Sulfurisphaera tokodaii

substrate_name: maltotriose

substrate: maltotriose


organism: Thermus thermophilus HB27

substrate_name: maltose

proof_lines:
wild-type, Vmax: 21 micromol/min mg
wild-type, Vmax: 357 micromol/min mg
wild-type, Vmax: 333 micromol/min mg

substrate: maltose


proof_lines: site-specific GK24 alpha-glucosidase mutant, Vmax: 3.4 micromol/min mg

proof_lines: site-specific mutant of HB27 alpha-glucosidase, Vmax: 1.9 micromol/min mg

proof_lines: site-specific GK24 alpha-glucosidase mutant, Vmax: 20 micromol/min mg


organism: Apis cerana indica

substrate_name: 4-nitrophenyl alpha-D-glucoside

substrate: 4-nitrophenyl alpha-D-glucoside


organism: Apis cerana japonica


organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4

substrate_name: maltopentaose

substrate: maltopentaose


organism: Ferroplasma acidiphilum


organism: Hordeum vulgare


organism: Saccharolobus solfataricus

proof_lines:
pH 4.5, 85°C, recombinant enzyme
pH 5.5, 85°C, recombinant enzyme


organism: Schizosaccharomyces pombe

substrate_name: kojibiose

substrate: kojibiose


organism: Anopheles aquasalis

proof_lines:
pH 6.5, 25°C, isozyme alphaGlu3
pH 6.5, 25°C, isozyme alphaGlu1
pH 6.5, 25°C, isozyme alphaGlu2


organism: Homo sapiens

substrate_name: kynuramine

assay_conditions_summary: pH 7.5; 298.15 K; buffer 50 mM phosphate

proof_lines: wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5)

substrate: kynuramine


proof_lines: mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5)


substrate_name: benzylamine

proof_lines: mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5)

substrate: benzylamine


organism: Aspergillus niger

substrate_name: p-nitrophenyl-alpha-D-glucopyranoside

sequence_source: UniProtKB:A2QJF7 live accession anchor; no sequence was loaded in this review.

substrate: p-nitrophenyl-alpha-D-glucopyranoside


organism: Candida albicans

sequence_source: UniProtKB:Q2V621 live accession anchor; contradicted enzyme/EC context.


sequence_source: UniProtKB:P10253 live accession anchor; no sequence was loaded in this review.


organism: Thermoplasma acidophilum

sequence_source: UniProtKB:Q9HLD0 live accession anchor; protein/EC annotation unavailable in the direct check.


organism: Levilactobacillus brevis

assay_conditions_summary: pH 7; 303.15 K; cofactor NADP+

proof_lines: reduction, recombinant mutant G37D, 30°C, pH 7.0

proof_lines: oxidation, cosubstrate NADP+, recombinant mutant G37D, 30°C, pH 7.0

proof_lines: oxidation, cosubstrate NAD+, recombinant mutant G37D, 30°C, pH 7.0


substrate_name: acetophenone

assay_conditions_summary: pH 7; 303.15 K; cofactor NADH

proof_lines: cofactor NADH, reduction, recombinant mutant G37Dm 30°C, pH 7.0

substrate: acetophenone


substrate_name: Phenylethanol

proof_lines: oxidation, cosubstrate NADP+, recombinant wild-type, 30°C, pH 7.0

proof_lines: cofactor NADPH, reduction, recombinant wild-type, 30°C, pH 7.0

substrate: Phenylethanol


organism: Bacillus subtilis

substrate_name: 7-aminocephalosporanic acid

proof_lines: D445G mutant, pH and temperature not specified in the publication

substrate: 7-aminocephalosporanic acid


proof_lines: E423/E442Q/D445N mutant, pH and temperature not specified in the publication


proof_lines: pH and temperature not specified in the publication


organism: Aspergillus awamori

substrate_name: alpha-naphthylbutyrate

substrate: alpha-naphthylbutyrate


organism: Trypanosoma cruzi

substrate_name: IMP

substrate: IMP


substrate_name: diphosphate

substrate: diphosphate


proof_lines: pH 7.5, 37°C, recombinant wild-type enzyme, with GMP


organism: Alkalihalophilus pseudofirmus


organism: Saccharomyces cerevisiae


organism: Glycine max

substrate_name: formate

assay_conditions_summary: pH 8; 310.15 K; cofactor NAD+

proof_lines: mutant enzyme A267M/I272V/F290N, at pH 8.0 and 37°C

substrate: formate


proof_lines: mutant enzyme A267M/I272V/F290D, at pH 8.0 and 37°C


organism: Pseudomonas sp.

substrate_name: cephalosporin C

substrate: cephalosporin C


organism: Trichomonas vaginalis

proof_lines:
isozyme 1, pH 4.5, 37°C
isozyme 2, pH 5.5, 37°C


substrate_name: NAD+

proof_lines: mutant enzyme F311N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication

substrate: NAD+


proof_lines: mutant enzyme F311D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


organism: Pyrococcus furiosus

substrate_name: NADP+

substrate: NADP+


organism: Penicillium citrinum

substrate_name: methyl 4-bromo-3-oxobutanoate

substrate: methyl 4-bromo-3-oxobutanoate


organism: Vanderwaltozyma polyspora

proof_lines: pH 9.5, 30°C, recombinant mutant V84I/Y127C, with 2-hydroxytetrahydrofuran


proof_lines: pH 9.5, 30°C, recombinant mutant V84I, with 2-hydroxytetrahydrofuran


organism: Camelus dromedarius

substrate_name: maltose

proof_lines:
pH 5.5, 37°C, isozyme MG6
pH 5.5, 37°C, isozyme MG4

substrate: maltose


enzyme_display_name: sucrose synthase

organism: Glycyrrhiza uralensis

substrate_name: UDP

mutation_signature: Δ9

proof_lines: mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C

proof_lines: mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C

enzyme: sucrose synthase

substrate: UDP


proof_lines: wild type enzyme isoform SUS1, at pH 5.5 and 50°C

proof_lines: wild type enzyme isoform SUS2, at pH 5.5 and 50°C


organism: Micractinium conductrix


organism: Aspergillus nidulans

substrate_name: maltotriose

substrate: maltotriose


organism: Leishmania donovani

substrate_name: guanine

substrate: guanine


organism: Bungarus fasciatus

substrate_name: acetylthiocholine

substrate: acetylthiocholine


organism: Plasmodium falciparum

substrate_name: xanthine

proof_lines: pH 7.4, 22°C, recombinant wild-type enzyme, with hypoxanthine

proof_lines: pH 7.4, 22°C, recombinant wild-type enzyme, with xanthine

substrate: xanthine


proof_lines: pH 7.4, 22°C, recombinant mutant L44F, with hypoxanthine

proof_lines: pH 7.4, 22°C, recombinant mutant L44F, with xanthine


organism: Bacteroides intestinalis

substrate_name: methyl ferulate

proof_lines: recombinant wild-type enzyme, pH 6.0, 45°C

substrate: methyl ferulate


organism: Mus musculus

substrate_name: Acetylcholine

substrate: Acetylcholine


proof_lines:
Y124C-acrylodan mutant
Y124C-SO3- mutant
Y124C-benzyl mutant


organism: Ferroplasma acidiphilum

substrate_name: sucrose

substrate: sucrose


substrate_name: maltotetraose

proof_lines: 60°C, pH not specified in the publication

substrate: maltotetraose


enzyme_display_name: furin

organism: synthetic construct

substrate_name: viral precursor protein E3E2

proof_lines: R64A mutant peptide of E3E2 from Asian Chikungunya virus strain

enzyme: furin

substrate: viral precursor protein E3E2


proof_lines: wild-type E3E2 from Asian Chikungunya virus strain


enzyme_display_name: cathepsin L

organism: Fasciola hepatica

proof_lines: mutant enzyme L69W, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C

enzyme: cathepsin L