enzyme_display_name: dCTP deaminase

organism: Bacillus subtilis

substrate_name: dCTP

enzyme: dCTP deaminase

substrate: dCTP


organism: Methanocaldococcus jannaschii

substrate_name: Unknown

source_db: brenda

kcat_display: Not reported

km_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

source_license: CC BY 4.0

ki_unit: mM

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

substrate: Unknown

source: brenda


enzyme_display_name: 6-aminohexanoate-oligomer endohydrolase

organism: Arthrobacter sp.

ki_display: Not reported

km_unit: mM

proof_lines: pH and temperature not specified in the publication

enzyme: 6-aminohexanoate-oligomer endohydrolase


organism: Pseudomonas sp.


enzyme_display_name: o-pyrocatechuate decarboxylase

organism: Aspergillus niger

enzyme: o-pyrocatechuate decarboxylase


organism: Aspergillus oryzae


organism: Cutaneotrichosporon cutaneum


organism: Klebsiella pneumoniae


organism: Avena sativa

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)


enzyme_display_name: 20-O-multi-glycoside ginsenosidase

substrate_name: ginsenoside Rb1

proof_lines:
free enzyme, at pH 5.5 and 40°C
immobilized enzyme, at pH 5.5 and 40°C

enzyme: 20-O-multi-glycoside ginsenosidase

substrate: ginsenoside Rb1


organism: Bifidobacterium breve

substrate_name: ginsenoside Rd

substrate: ginsenoside Rd


organism: Bifidobacterium longum

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

proof_lines:
at pH 7.2 and 37°C
at pH 6.0 and 37°C

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Lissachatina fulica


organism: Paenibacillus polymyxa


organism: Pseudarthrobacter chlorophenolicus


organism: Thermotoga petrophila


organism: Crithidia fasciculata

substrate_name: R'C(R)SH; t-Butylhydroperoxide

protein_accession_database: UniProt

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

substrate: R'C(R)SH; t-Butylhydroperoxide


organism: Fasciola hepatica

source_db: oed

smiles: OO

source: oed


substrate_name: cumene hydroperoxide

substrate: cumene hydroperoxide


substrate_name: H2O2; Reduced thioredoxin

substrate: H2O2; Reduced thioredoxin


organism: Leishmania donovani


organism: Leishmania infantum


organism: Trypanosoma brucei brucei

substrate_name: t-Butylhydroperoxide

smiles: CC(C)(C)OO

substrate: t-Butylhydroperoxide


organism: Trypanosoma cruzi


enzyme_display_name: flavonoid 3'-monooxygenase

organism: Arabidopsis thaliana

substrate_name: dihydrokaempferol

enzyme: flavonoid 3'-monooxygenase

substrate: dihydrokaempferol


substrate_name: kaempferol

substrate: kaempferol


organism: Oryza sativa Japonica Group

proof_lines:
pH 7.5, 30°C, enzyme from white rice
pH 7.5, 30°C, enzyme from black rice


organism: Tagetes erecta

substrate_name: naringenin

substrate: naringenin


substrate_name: apigenin

substrate: apigenin


organism: Mycobacterium tuberculosis

source_license: CC BY 4.0; CC BY-NC-ND 4.0

proof_lines:
pH 7.5, 25°C
Raw SKiD Env: Temp=25°C, pH=7.5

source_databases_merged: brenda skid


organism: Mycolicibacterium smegmatis

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution

proof_lines: mutant Y87W, pH 7.4, temperature not specified in the publication


proof_lines: mutant W87Y, pH 7.4, temperature not specified in the publication


proof_lines: wild-type, pH 7.4, temperature not specified in the publication


organism: Camellia sinensis


organism: Dahlia pinnata

proof_lines:
allele 2, pH 7.5, 30°C
allele 1, pH 7.5, 30°C


organism: Fragaria x ananassa


enzyme_display_name: phosphooxymethylpyrimidine kinase

organism: Escherichia coli

proof_lines: pH 7.5, temperature not specified in the publication, recombinant enzyme ThiD

enzyme: phosphooxymethylpyrimidine kinase


organism: Sulfurimonas denitrificans

proof_lines: pH 7.5, temperature not specified in the publication, recombinant enzyme ThiD2


enzyme_display_name: phosphatidylglycerophosphatase

enzyme: phosphatidylglycerophosphatase


organism: Bacteroides ovatus

substrate_name: Candida mannan

substrate: Candida mannan


organism: Bacteroides thetaiotaomicron


organism: Dyadobacter fermentans


enzyme_display_name: DNA-deoxyinosine glycosylase

organism: Pedobacter heparinus

substrate_name: uracil-containing double-stranded DNA

proof_lines:
G/U pairing, pH 7.5, 37°C
T/hypoxanthine pairing, pH 7.5, 37°C
A/U pairing, pH 7.5, 37°C

enzyme: DNA-deoxyinosine glycosylase

substrate: uracil-containing double-stranded DNA


enzyme_display_name: peptidase Do

organism: Homo sapiens

substrate_name: beta-casein

enzyme: peptidase Do

substrate: beta-casein


proof_lines: pH not specified in the publication, at 37°C


organism: Plasmodium falciparum

assay_conditions_summary: pH 7

proof_lines: pH 7.0, temperature not specified in the publication


organism: Entamoeba histolytica

proof_lines: pH 5.5, 37°C, recombinant mutant D46A cytoplasmic catalytic domain, no metal added

proof_lines: pH 5.5, 37°C, recombinant mutant D46A cytoplasmic catalytic domain, metal added


enzyme_display_name: nitrate reductase (quinone)

substrate_name: nitrate

enzyme: nitrate reductase (quinone)

substrate: nitrate


enzyme_display_name: adenosine tuberculosinyltransferase

substrate_name: tuberculosinyl diphosphate

enzyme: adenosine tuberculosinyltransferase

substrate: tuberculosinyl diphosphate


organism: Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4)

source_db: uniprot

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: Characterized with purified enzyme corresponding to a dimer of NarG and NarH.

source: uniprot


enzyme_display_name: (E3-independent) E2 ubiquitin-conjugating enzyme

substrate_name: beta-catenin-monoubiquitin

proof_lines: Measured for E2-E3 complex composed of neddylated UBE2R1 and the CRL2(FEM1C) complex, using an 'Arg-48' donor ubiquitin.

substrate: beta-catenin-monoubiquitin


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: tuberculosinyl diphosphate (at 45 degrees Celsius and pH 7)

proof_lines: kcat is 0.031 min(-1) with tuberculosinyl diphosphate as substrate.


enzyme_display_name: GTP 3',8-cyclase

proof_lines: kcat is 0.092 min(-1) for (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.

enzyme: GTP 3',8-cyclase


organism: Staphylococcus aureus (strain NCTC 8325 / PS 47)

substrate_name: GTP

substrate: GTP


enzyme_display_name: orcinol 2-monooxygenase

organism: Pseudomonas putida

substrate_name: orcinol

assay_conditions_summary: cofactor NADPH

proof_lines:
crystalline enzyme
cofactor: NADH
cofactor: 3-acetylpyridine NADH

enzyme: orcinol 2-monooxygenase

substrate: orcinol


organism: Citrus sinensis


organism: Columnea hybrida


organism: Torenia sp.


enzyme_display_name: asparagusate reductase

organism: Asparagus officinalis

substrate_name: potassium ferricyanide

proof_lines:
enzyme I
enzyme II
enzyme I and II

enzyme: asparagusate reductase

substrate: potassium ferricyanide


organism: Escherichia coli

substrate_name: ATP

assay_conditions_summary: pH 8; 298.15 K; cofactor ATP

substrate: ATP


organism: Oryctolagus cuniculus

substrate_name: [histone H2A]-L-lysine

proof_lines:
E2 enzyme of 20 kDa, pH 7.5, 37°C
pH 7.0, 37°C, Vmax, 0.06 microM
pH 7.0, 37°C, Vmax, 0.12 microM

enzyme: (E3-independent) E2 ubiquitin-conjugating enzyme

substrate: [histone H2A]-L-lysine


proof_lines:
pH 7
pH 7.0
pH 7.0, competitive inhibition constant


organism: Escherichia coli K-12


organism: Mycobacterium tuberculosis

substrate_name: cholesterol

proof_lines: pH and temperature not specified in the publication

proof_lines: apparent value, Km above 1.2 mM, in 0.1 M potassium phosphate at pH 7.0, temperature not specified in the publication

proof_lines: apparent value, in 0.1 M potassium phosphate at pH 7.0, temperature not specified in the publication

substrate: cholesterol


organism: Bos taurus

substrate_name: poly(dAdT)

substrate: poly(dAdT)


organism: Bacillus licheniformis

substrate_name: phosphatidylglycerophosphate

substrate: phosphatidylglycerophosphate


organism: Rattus sp.


organism: Saccharomyces cerevisiae


enzyme_display_name: venombin AB

organism: Bitis gabonica

substrate_name: Tosyl-Gly-Pro-Arg 4-nitroanilide

enzyme: venombin AB

substrate: Tosyl-Gly-Pro-Arg 4-nitroanilide


organism: Vigna radiata

substrate_name: dipalmitoyl sn-glycero 3-phosphate

substrate: dipalmitoyl sn-glycero 3-phosphate


organism: Homo sapiens


organism: Streptococcus pneumoniae

substrate_name: CSP-1 FRET peptide

substrate: CSP-1 FRET peptide


enzyme_display_name: pantothenase

organism: Pseudomonas fluorescens

substrate_name: pantothenic acid

proof_lines: 10 mM Tris/10 mM citric acid(KOH), pH 7.5

enzyme: pantothenase

substrate: pantothenic acid


enzyme_display_name: Ste24 endopeptidase

organism: Saccharomyces mikatae

substrate_name: Abz-KSKTKC(farnesyl)VIKD

proof_lines: in the absence of insulin B chain, at pH 7.5 and 30°C

proof_lines: in the presence of 0.02 mM insulin B chain, at pH 7.5 and 30°C

enzyme: Ste24 endopeptidase

substrate: Abz-KSKTKC(farnesyl)VIKD


organism: Escherichia coli (strain K12)

substrate_name: RseA-YQF

substrate: RseA-YQF


enzyme_display_name: prolyl oligopeptidase

substrate_name: Gly-Pro (Prolyl endopeptidase activity)

enzyme: prolyl oligopeptidase

substrate: Gly-Pro (Prolyl endopeptidase activity)


organism: Dictyostelium discoideum

substrate_name: carbobenzoxy-Gly-Pro-p-nitroanilide (at 37 degrees Celsius)

verification_status: verified

column_label: Table I, page 2738, row Z-Gly-Pro-pNA / Dictyostelium DpoA, Km column: 115 +/- 13 uM. Methods page 2742 gives the assay conditions. Figure 3, pages 2736-2737, prints the full dpoA cDNA and predicted 760-aa product from wild-type Ax2 cells.

normalized_value_unit_evidence: Km normalized_value: 0.115 mM from the printed 115 uM value; it exactly matches the current source-aligned preimage.

raw_value_unit_evidence: Table I prints Km = 115 +/- 13 uM for Z-Gly-Pro-pNA with Dictyostelium DpoA.

row_label: Z-Gly-Pro-pNA / Dictyostelium DpoA

table_label: Table I

status: verified


organism: Galerina marginata (strain CBS 339.88)

substrate_name: the full alpha-amanitin 35mer proprotein

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


substrate_name: Tosyl-L-arginine methyl ester (TAME)

sequence: VVGGAECKIDGHRCLALLY

wild_type_sequence: VVGGAECKIDGHRCLALLY

substrate: Tosyl-L-arginine methyl ester (TAME)


enzyme_display_name: N-methylhydantoinase (ATP-hydrolyzing)

organism: Pseudomonas putida

substrate_name: dihydrouracil

enzyme: N-methylhydantoinase (ATP-hydrolyzing)

substrate: dihydrouracil


organism: Entamoeba histolytica

assay_conditions_summary: pH 5.5; 310.15 K; buffer 50 mM acetate

proof_lines: in the absence of metal ions, in 50 mM acetate buffer at pH 5.5 and 37°C

proof_lines: in the presence of 5 mM Mn2+, in 50 mM acetate buffer at pH 5.5 and 37°C


proof_lines:
presence of 5 mM Mg2+, pH 6.5, 30°C
pH 6.5, 30°C


organism: Trypanosoma sp.


organism: Staphylococcus aureus


organism: Unknown

substrate_name: GDPmannose

substrate: GDPmannose


enzyme_display_name: phosphoribosylanthranilate isomerase

enzyme: phosphoribosylanthranilate isomerase


organism: Thermotoga maritima

proof_lines: pH 7.5, 25°C, D130P, mutant form of imidazole glycerol phosphate synthase


proof_lines: pH 7.5, 25°C, D130T, mutant form of imidazole glycerol phosphate synthase


proof_lines: pH 7.5, 25°C, D127K, mutant form of EC 5.3.1.6


proof_lines: pH 7.5, 25°C, D127G, mutant form of EC 5.3.1.6


proof_lines: mutant enzyme D130V, in 50 mM HEPES, pH 7.5, at 25°C

proof_lines: pH 7.5, 25°C, D130V, mutant form of imidazole glycerol phosphate synthase


proof_lines: mutant enzyme D127V, in 50 mM HEPES, pH 7.5, at 25°C

proof_lines: pH 7.5, 25°C, D127V, mutant form of EC 5.3.1.6


proof_lines: mutant enzyme D127V/D173V, in 50 mM HEPES, pH 7.5, at 25°C


proof_lines: mutant enzyme D173V, in 50 mM HEPES, pH 7.5, at 25°C


proof_lines: mutant enzyme D127V/D169V, in 50 mM HEPES, pH 7.5, at 25°C


proof_lines: wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C


proof_lines: mutant enzyme D130V/D176V, in 50 mM HEPES, pH 7.5, at 25°C


proof_lines: mutant enzyme D169V, in 50 mM HEPES, pH 7.5, at 25°C


proof_lines: pH 7.5, 25°C, D127V/T164H, mutant form of EC 5.3.1.6


proof_lines: pH 7.5, 25°C, D127T, mutant form of EC 5.3.1.6


proof_lines: pH 7.5, 25°C, D127F, mutant form of EC 5.3.1.6


organism: Phaseolus lunatus

substrate_name: trans-zeatin

substrate: trans-zeatin


enzyme_display_name: streptomycin 6-kinase

organism: Escherichia coli

substrate_name: ATP

assay_conditions_summary: pH 7.5; 298.15 K; cofactor ATP

enzyme: streptomycin 6-kinase


organism: Streptomyces griseus

assay_conditions_summary: cofactor ATP


organism: Cucumis sativus

substrate_name: diphosphate

proof_lines:
pH 7.2, 30°C
phosphate in form of Mg-diphosphate

substrate: diphosphate


substrate_name: alpha-D-glucose 1-phosphate

substrate: alpha-D-glucose 1-phosphate


organism: Homo sapiens

substrate_name: UDP-galactose

proof_lines:
healthy persons, pH 7.8, 37°C
galactosemic individuals, pH 7.8, 37°C
healthy individuals, pH 7.8, 37°C

substrate: UDP-galactose


organism: Bos taurus


enzyme_display_name: itaconyl-CoA hydratase

organism: Pseudomonas aeruginosa

assay_conditions_summary: pH 7.5; 318.15 K; cofactor CoA

enzyme: itaconyl-CoA hydratase


organism: Yersinia pestis

substrate_name: (S)-citramalyl-CoA

substrate: (S)-citramalyl-CoA


organism: Pseudomonas fluorescens

substrate_name: Salicylaldehyde

substrate: Salicylaldehyde


organism: Rhodococcus opacus

substrate_name: trans-o-hydroxybenzylidenepyruvate

proof_lines: pH and temperature not specified in the publication

substrate: trans-o-hydroxybenzylidenepyruvate


organism: Sphingobium xenophagum


organism: Sphingomonas paucimobilis

proof_lines:
30°C, pH 8.1, enzyme form fHBP HA A
30°C, pH 8.1, enzyme form fHBP HA B


organism: Ogataea henricii


organism: Saccharomyces cerevisiae

proof_lines: pH 7.5, 25°C, recombinant xPRAI, expressed in E. coli

proof_lines: pH 7.5, 25°C, recombinant xPRAI, expressed in Escherichia coli


proof_lines:
pH 7.5, 45°C, dimer
pH 7.5, 80°C, dimer
pH 7.5, 60°C, dimer


enzyme_display_name: phosphatidate cytidylyltransferase

substrate_name: phosphatidate

enzyme: phosphatidate cytidylyltransferase

substrate: phosphatidate


organism: Bacillus subtilis

substrate_name: CTP; Phosphatidate

substrate: CTP; Phosphatidate


proof_lines:
pH 8.0, 22°C
pH 8.0, 22°C, recombinant CDS2


proof_lines:
pH 8.0, 22°C
pH 8.0, 22°C, recombinant CDS1


organism: Rattus norvegicus


proof_lines: with NBD-phosphatidate, pH and temperature not specified in the publication


organism: Schizosaccharomyces pombe

substrate_name: CTP

substrate: CTP


substrate_name: Unknown

proof_lines:
at pH 7.5 and 25°C
Ki above 0.05 mM, at pH 7.5 and 25°C

substrate: Unknown


organism: Entamoeba histolytica

proof_lines: pH 5.5, 37°C, recombinant mutant H140A cytoplasmic catalytic domain, no metal added

proof_lines: pH 5.5, 37°C, recombinant mutant H140A cytoplasmic catalytic domain, metal added


proof_lines: pH 5.5, 37°C, recombinant mutant H43A cytoplasmic catalytic domain, no metal added

proof_lines: pH 5.5, 37°C, recombinant mutant H43A cytoplasmic catalytic domain, metal added


proof_lines: pH 5.5, 37°C, recombinant wild-type cytoplasmic catalytic domain, no metal added

proof_lines: pH 5.5, 37°C, recombinant wild-type cytoplasmic catalytic domain, metal added


enzyme_display_name: 5'-deoxyadenosine deaminase

organism: Methanocaldococcus jannaschii

proof_lines: pH and temperature not specified in the publication, mutant enzyme Y136R/E150R

enzyme: 5'-deoxyadenosine deaminase


proof_lines: pH and temperature not specified in the publication, wild-type enzyme


enzyme_display_name: 22alpha-hydroxysteroid 23-monooxygenase

organism: Arabidopsis thaliana

substrate_name: NADPH; O2; 22alpha-Hydroxy-campesterol; H+

enzyme: 22alpha-hydroxysteroid 23-monooxygenase


substrate: NADPH; O2; 22alpha-Hydroxy-campesterol; H+


organism: Mycobacterium tuberculosis

substrate_name: UDP-glucose; Glucose 6-phosphate

substrate: UDP-glucose; Glucose 6-phosphate


substrate_name: D-Glucose 6-phosphate; UDPglucose

substrate: D-Glucose 6-phosphate; UDPglucose


organism: Sulfolobus acidocaldarius

substrate_name: UDP-alpha-D-glucose

substrate: UDP-alpha-D-glucose


organism: Thermoplasma acidophilum

substrate_name: D-glucose 6-phosphate

substrate: D-glucose 6-phosphate


mutation_signature: N-loop truncation mutant enzyme

mutation_type: truncation

proof_lines: pH 6.0, 60°C, N-loop truncation mutant enzyme


organism: Thermus thermophilus

substrate_name: UDP-glucose

proof_lines: recombinant enzyme, pH 7.0, 80°C, in presence of Mg2+

substrate: UDP-glucose


organism: Phaseolus lunatus

substrate_name: UDP-D-glucose; Zeatin

substrate: UDP-D-glucose; Zeatin


enzyme_display_name: succinylornithine transaminase

organism: Escherichia coli

substrate_name: L-ornithine

assay_conditions_summary: pH 8

proof_lines: pH 8.0, temperature not specified in the publication

enzyme: succinylornithine transaminase

substrate: L-ornithine


enzyme_display_name: mitogen-activated protein kinase kinase

organism: Homo sapiens

proof_lines:
pH 7.0, 25°C
Raw SKiD Env: Temp=25°C, pH=7

enzyme: mitogen-activated protein kinase kinase


proof_lines: kcat is 0.12 min(-1) for (22S)-22-hydroxycampesterol (PubMed:17138693). kcat is 1.27 min(-1) for (22S,24R)-22-hydroxy-5-alpha-ergostan-3-one (PubMed:17138693). kcat is 1.01 min(-1) for (22S,24R)-22-hydroxyergost-4-en-3-one (PubMed:17138693)


proof_lines: kcat is 0.11 min(-1) for (22S)-22-hydroxycampesterol (PubMed:17138693). kcat is 2.75 min(-1) for (22S,24R)-22-hydroxy-5-alpha-ergostan-3-one (PubMed:17138693). kcat is 1.40 min(-1) for (22S,24R)-22-hydroxyergost-4-en-3-one (PubMed:17138693)


organism: Escherichia coli (strain K12)

substrate_name: UDP-Glc

proof_lines: kcat is 34 sec(-1) for glucosyltransferase activity.

substrate: UDP-Glc


organism: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

substrate_name: 5'-deoxyadenosine (at pH 9 and 60 degrees Celsius)

assay_conditions_summary: pH 9

proof_lines: kcat is 120000 sec(-1) for 5'-deoxyadenosine deamination. kcat is 160 sec(-1) for S-methyl-5'-thioadenosine deamination. kcat is 1300 sec(-1) for S-adenosyl-L-homocysteine deamination. kcat is 110 sec(-1) for adenosine deamination (at pH 9


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)


organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843)


enzyme_display_name: GDP polyribonucleotidyltransferase

organism: Vesicular stomatitis Indiana virus (strain San Juan)

enzyme: GDP polyribonucleotidyltransferase


organism: Bos taurus

substrate_name: UDP-N-acetyl-alpha-D-glucosamine

substrate: UDP-N-acetyl-alpha-D-glucosamine


enzyme_display_name: phosphatidylglycerophosphatase

substrate_name: phosphatidylglycerophosphate

enzyme: phosphatidylglycerophosphatase

substrate: phosphatidylglycerophosphate


substrate_name: undecaprenyl pyrophosphate

substrate: undecaprenyl pyrophosphate


enzyme_display_name: [myosin-light-chain] phosphatase

organism: Oryctolagus cuniculus

substrate_name: phosphorylase

enzyme: [myosin-light-chain] phosphatase

substrate: phosphorylase


enzyme_display_name: fructan beta-(2,1)-fructosidase

organism: Triticum aestivum

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

enzyme: fructan beta-(2,1)-fructosidase


organism: Sphingobium xenophagum

substrate_name: 2,6-DHBP (at pH 7)

substrate: 2,6-DHBP (at pH 7)


assay_conditions_summary: cofactor CoA


substrate_name: glucose 6-phosphate

proof_lines: bifunctional fusion enzyme of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase

substrate: glucose 6-phosphate


organism: Halorhodospira halochloris

substrate_name: UDPglucose

substrate: UDPglucose


organism: Hyalophora cecropia


organism: Metarhizium anisopliae


organism: Pleurotus ostreatus


organism: Propionibacterium freudenreichii

assay_conditions_summary: pH 5.5; 310.15 K; cofactor ADP

proof_lines:
37°C, pH 5.5
37°C, pH 5.5, cosubstrate: TDP-glucose
37°C, pH 5.5, cosubstrate: GDP-glucose


organism: Burkholderia cepacia


organism: Pseudomonas aeruginosa


organism: Bacillus subtilis

substrate_name: phosphatidic acid

proof_lines:
pH 7.5, 25°C, with 0.45 mM CTP
pH 7.5, 25°C

substrate: phosphatidic acid


organism: Catharanthus roseus

substrate_name: CTP

substrate: CTP


organism: Cavia porcellus

substrate_name: phosphatidate

substrate: phosphatidate


organism: Clostridium perfringens


organism: Saccharomyces cerevisiae

substrate_name: D-Glucose 6-phosphate; UDPglucose

substrate: D-Glucose 6-phosphate; UDPglucose


organism: Escherichia coli


organism: Sus scrofa


substrate_name: dehydroepiandrosterone

proof_lines: inhibition of hydroxylation of dehydroepiandrosterone

substrate: dehydroepiandrosterone


organism: Homo sapiens


organism: Rattus norvegicus


enzyme_display_name: 1,8-cineole 2-exo-monooxygenase

proof_lines: pH 7.4, 37°C, formation of 2-hydroxy-1,8-cineole by isoform CYP3A4

proof_lines: pH 7.4, 37°C, formation of 3-hydroxy-1,8-cineole by isoform CYP3A4

enzyme: 1,8-cineole 2-exo-monooxygenase


proof_lines: pH 7.4, 37°C, after treatment of animals with pregenolone-16alpha-carbonitrile

proof_lines: pH 7.4, 37°C, animals treated with pregnenolone 16alpha-carbonitrile


enzyme_display_name: choloyl-CoA hydrolase

organism: Mus musculus

substrate_name: acetyl-CoA

proof_lines: In summary, KM for medium- to long-chain acyl CoAs is in the order 1.4-6.7 uM, and with short-chain acyl CoAs range from 8 to 30 uM. KM for bile acid-CoA esters is in the range 6-15 uM.

enzyme: choloyl-CoA hydrolase

substrate: acetyl-CoA


organism: Eubacterium sp.

substrate_name: cholyl-CoA

assay_conditions_summary: pH 7; 310.15 K; cofactor CoA

substrate: cholyl-CoA


substrate_name: dithiothreitol

assay_conditions_summary: pH 8; 310.15 K; cofactor CoA

proof_lines: pH 8.0, 37°C, with chenodeoxycholoyl-CoA

proof_lines: pH 8.0, 37°C, postnuclear fraction of liver homogenate

substrate: dithiothreitol


substrate_name: taurine toward choloyl-CoA

substrate: taurine toward choloyl-CoA


substrate_name: decanoyl-CoA

substrate: decanoyl-CoA


organism: Lithospermum erythrorhizon


organism: Pinus densiflora


organism: Punica granatum

substrate_name: gallic acid (at pH 5.0 and 30 degrees Celsius)

assay_conditions_summary: pH 5

proof_lines: kcat is 0.55 sec(-1) with gallic acid as substrate. kcat is 0.6 sec(-1) with 4-hydroxybenzoic acid as substrate. kcat is 1.35 sec(-1) with 3,4-dihydroxybenzoic acid as substrate. kcat is 0.89 sec(-1) with caffeic acid as substrate. kcat is


proof_lines: kcat is 0.52 sec(-1) with gallic acid as substrate. kcat is 0.61 sec(-1) with 4-hydroxybenzoic acid as substrate. kcat is 0.76 sec(-1) with 3,4-dihydroxybenzoic acid as substrate. kcat is 0.64 sec(-1) with caffeic acid as substrate. kcat is

substrate: gallic acid (at pH 5.0 and 30 degrees Celsius)


organism: Paenibacillus borealis

proof_lines: kcat is 102 sec(-1) with O-beta-D-glucosyl-D-glucuronate as substrate (PubMed:35947916). kcat is 156 sec(-1) with alpha-D-glucose 1-phosphate as substrate (PubMed:35947916). kcat is 351 sec(-1) with D-glucuronic acid as substrate (PubMed:35


enzyme_display_name: guanosine deaminase

organism: Arabidopsis thaliana

enzyme: guanosine deaminase


organism: Camellia sinensis

substrate_name: guanosine

substrate: guanosine


organism: Pseudomonas putida

substrate_name: deoxyguanosine

substrate: deoxyguanosine


enzyme_display_name: leukotriene-C4 hydrolase

substrate_name: glutathione

enzyme: leukotriene-C4 hydrolase

substrate: glutathione


substrate_name: glycylglycine

substrate: glycylglycine


substrate_name: leukotriene C(4)

substrate: leukotriene C(4)


substrate_name: reduced glutathione

proof_lines: in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C

substrate: reduced glutathione


substrate_name: S-decyl-GSH

assay_conditions_summary: pH 8; 310.15 K; buffer 0.1 M Tris-HCl

proof_lines: in 0.1 M Tris-HCl buffer, pH 8.0, at 37°C

proof_lines: pH and temperature not specified in the publication

substrate: S-decyl-GSH


enzyme_display_name: creatinine deaminase

organism: Clostridium paraputrificum

substrate_name: creatinine

enzyme: creatinine deaminase

substrate: creatinine


organism: Corynebacterium glutamicum

proof_lines:
pH 7.5, 37°C
soluble enzyme
immobilized enzyme


organism: Cryptococcus neoformans


organism: Flavobacterium filamentosum

substrate_name: cytosine

substrate: cytosine


organism: uncultured bacterium


organism: Unknown

substrate_name: UDPglucose

substrate: UDPglucose


enzyme_display_name: NAD-dependent lipoamidase

substrate_name: [histone H3]-N6-acetyl-L-lysine

enzyme: NAD-dependent lipoamidase

substrate: [histone H3]-N6-acetyl-L-lysine


enzyme_display_name: caffeine synthase

organism: Coffea arabica

enzyme: caffeine synthase


substrate_name: S-Adenosyl-L-methionine; Theobromine

substrate: S-Adenosyl-L-methionine; Theobromine


enzyme_display_name: [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M

organism: Methanosarcina barkeri

proof_lines:
isozyme MT2-M, pH 7.2, 23°C
isozyme MT2-A, pH 7.2, 23°C
isozymes MT2-A and MT-2-M, pH 7.2, 23°C


organism: Ricinus communis

substrate_name: [histone H3.1]-L-lysine27

substrate: [histone H3.1]-L-lysine27


mutation_signature: PHD domain deletion mutant

mutation_type: deletion

proof_lines: PHD domain deletion mutant, pH 8.0, 30°C


enzyme_display_name: cocaine synthase

organism: Erythroxylum coca

substrate_name: ecgonine methyl ester

assay_conditions_summary: pH 9.4; 293.15 K; cofactor CoA

proof_lines: with benzoyl-CoA as sosubstrate, at pH 9.4 and 20°C

proof_lines: with cinnamoyl-CoA as sosubstrate, at pH 9.4 and 20°C

enzyme: cocaine synthase

substrate: ecgonine methyl ester


organism: Mesocricetus auratus


organism: Rattus norvegicus


enzyme_display_name: phosphoribosylanthranilate isomerase

organism: Streptomyces coelicolor

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

proof_lines:
PriA, PRA isomerase activity
Raw SKiD Env: Temp=-----, pH=-----

source_databases_merged: sabio_rk brenda skid

enzyme: phosphoribosylanthranilate isomerase


proof_lines: PriA mutant R19A, PRA isomerase activity


organism: Thermotoga maritima


enzyme_display_name: 5-formyltetrahydrofolate cyclo-ligase

organism: Arabidopsis thaliana

mutation_signature: truncated recombinant enzyme

proof_lines: truncated recombinant enzyme, pH 6.0, 23°C

enzyme: 5-formyltetrahydrofolate cyclo-ligase


organism: Salmonella enterica subsp. enterica serovar Typhimurium


organism: Paenibacillus borealis

substrate_name: 4-nitrophenyl beta-D-glucuronide

substrate: 4-nitrophenyl beta-D-glucuronide


enzyme_display_name: guanidinoacetate kinase

organism: Namalycastis sp.

substrate_name: glycocyamine

proof_lines:
recombinant GK beta
native GK

enzyme: guanidinoacetate kinase

substrate: glycocyamine


enzyme_display_name: alpha-mannan endo-1,2-alpha-mannanase

organism: Bacteroides thetaiotaomicron

enzyme: alpha-mannan endo-1,2-alpha-mannanase


organism: Bacteroides xylanisolvens


enzyme_display_name: 5'-deoxyadenosine deaminase

organism: Methanocaldococcus jannaschii

proof_lines: pH and temperature not specified in the publication, mutant enzyme Y136R

enzyme: 5'-deoxyadenosine deaminase


proof_lines: pH and temperature not specified in the publication, mutant enzyme E150R


enzyme_display_name: GTP 3',8-cyclase

organism: Staphylococcus aureus

substrate_name: GTP

enzyme: GTP 3',8-cyclase

substrate: GTP


substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


enzyme_display_name: D-glutamate cyclase

organism: Mus musculus

substrate_name: D-Glutamate

enzyme: D-glutamate cyclase

substrate: D-Glutamate


substrate_name: D-glutamate

proof_lines:
colorimetric assay, pH 8.0, 37°C
pH 8.0, 37°C

substrate: D-glutamate


enzyme_display_name: Transferred entry: 3.5.99.14

organism: Thalictrum flavum

source_db: skid

source_license: CC BY-NC-ND 4.0

enzyme: Transferred entry: 3.5.99.14

source: skid


organism: Thalictrum flavum subsp. glaucum


organism: Cucurbita pepo


organism: Malus domestica

substrate_name: S-Adenosyl-L-methionine

substrate: S-Adenosyl-L-methionine


organism: Vigna radiata


enzyme_display_name: adenylate cyclase

organism: Yersinia pestis

substrate_name: ATP

assay_conditions_summary: pH 8.5; 310.15 K; cofactor ATP

proof_lines: mutant enzyme L72A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme L72A, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C

enzyme: adenylate cyclase

substrate: ATP


proof_lines: mutant enzyme R113A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C


proof_lines: mutant enzyme M140A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme M140A, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C


proof_lines: mutant enzyme E12Q, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme E12Q, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C


proof_lines: mutant enzyme K14A, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme K14A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C


organism: Escherichia coli


organism: Geobacillus thermopakistaniensis


organism: Saccharomyces cerevisiae


organism: Silene dioica

substrate_name: UDP-glucose

proof_lines: reaction with cyanidin 3-rhamnosylglucoside at pH 7.4

substrate: UDP-glucose


organism: Nephtys caeca

substrate_name: ADP

substrate: ADP


organism: Perinereis sp.

assay_conditions_summary: pH 8.1; 298.15 K; cofactor ATP


enzyme_display_name: T2-induced deoxynucleotide kinase

substrate_name: dGMP

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

proof_lines:
pH 7.4, 37°C
pH 7.4, 37°C, with dTMP
pH 7.4, 37°C, with 5-hydroxymethyl-dCMP

enzyme: T2-induced deoxynucleotide kinase

substrate: dGMP


organism: Microbacterium sediminis

substrate_name: D-glucose 6-phosphate

substrate: D-glucose 6-phosphate


organism: Mycobacterium tuberculosis

proof_lines:
with UDPglucose as glucosyl donor
with GDPglucose as glucosyl donor


organism: Mycolicibacterium smegmatis

substrate_name: UDPglucose

proof_lines: in presence of optimal concentration of a polyanion

substrate: UDPglucose


organism: Neurospora crassa


organism: Homo sapiens

substrate_name: complement component C5

proof_lines: high affinity C5 convertase (EAC1,C3bC4b,C2a)

proof_lines: surface-bound C5 convertase (EAC1,C4b,C2a)

proof_lines: 37°C, soluble monomeric C3/C5 convertase C4b,C2a

substrate: complement component C5


organism: Mus musculus


enzyme_display_name: 4-acetamidobutyryl-CoA deacetylase

organism: Pseudomonas sp.

proof_lines: estimated from rates at lower and higher substrate concentration

enzyme: 4-acetamidobutyryl-CoA deacetylase


organism: Brevundimonas diminuta

substrate_name: N-myristoyl-L-glutamate

substrate: N-myristoyl-L-glutamate


organism: Penicillium sp.

substrate_name: N-oleoyl-L-glutamate

substrate: N-oleoyl-L-glutamate


enzyme_display_name: diguanidinobutanase

organism: Pseudomonas putida

enzyme: diguanidinobutanase


enzyme_display_name: gentisate 1,2-dioxygenase

organism: Corynebacterium glutamicum

enzyme: gentisate 1,2-dioxygenase


organism: Escherichia coli


organism: Pseudaminobacter salicylatoxidans

proof_lines: pH 8.0, temperature not specified in the publication


organism: Pseudomonas alcaligenes

substrate_name: O2; Gentisate

substrate: O2; Gentisate


organism: Rhodococcus opacus


organism: Ruegeria pomeroyi


organism: Xanthobacter polyaromaticivorans

substrate_name: 1-hydroxy 2 naphthoate

substrate: 1-hydroxy 2 naphthoate


organism: Aquipseudomonas alcaligenes

assay_conditions_summary: pH 7.4; 296.15 K; buffer 0.1 M phosphate

proof_lines: in 0.1 M phosphate buffer, pH 7.4, at 23°C


organism: Comamonas testosteroni

substrate_name: gentisate

substrate: gentisate


organism: Delftia acidovorans


organism: Faucicola osloensis


proof_lines: mutant enzyme Y181F, in 0.1 M KH2PO4 buffer (pH 7.4) at 23°C


proof_lines: wild type enzyme, in 0.1 M KH2PO4 buffer (pH 7.4) at 23°C


enzyme_display_name: UDP-glucuronate 4-epimerase

organism: Arabidopsis thaliana

substrate_name: UDPglucuronate

enzyme: UDP-glucuronate 4-epimerase

substrate: UDPglucuronate


substrate_name: UDP-glucuronate

proof_lines: recombinant AtUGlcAE3, lacking the amino acid position 1-53, in 0.1 M sodium phosphate, pH 7.6

proof_lines: recombinant AtUGlcAE3, position 1-53 is lacking

substrate: UDP-glucuronate


proof_lines: recombinant AtUGlcAE2, lacking the amino acid position 1-68, in 0.1 M sodium phosphate, pH 7.6

proof_lines: recombinant AtUGlcAE2, position 1-68 is lacking


organism: Oryza sativa

proof_lines: recombinant OsUGlcAE3, lacking the amino acid position 1-53, in 0.1 M sodium phosphate, pH 7.6

proof_lines: recombinant OsUGlcAE3, position 1-53 is lacking


organism: Zea mays

proof_lines: recombinant ZmUGlcAE, lacking the putative transmembrane domain at amino acid position 1-47, in 0.1 M sodium phosphate, pH 7.6

proof_lines: recombinant ZmUGlcAE, position 1-47 is lacking


enzyme_display_name: alpha-methylacyl-CoA racemase

organism: Homo sapiens

substrate_name: (S)-Pristanoyl-CoA

assay_conditions_summary: pH 7.2; 303.15 K; cofactor CoA

proof_lines: recombinant enzyme, 50 mM NaH2PO4-NaOH, pH 7.2

proof_lines: pH 7.4, 30°C, recombinant human AMACR 1A

proof_lines: pH not specified in the publication, 37°C

enzyme: alpha-methylacyl-CoA racemase

substrate: (S)-Pristanoyl-CoA


organism: Rattus norvegicus


substrate_name: salicylate

substrate: salicylate


proof_lines: wild-type enzyme, pH and temperature not specified in the publication


organism: Pseudomonas putida

assay_conditions_summary: pH 7.4; 296.15 K; buffer assay context


organism: Ralstonia solanacearum


organism: Anabaena flos-aquae


organism: Bacillus cereus

substrate_name: UDP-D-galacturonate

substrate: UDP-D-galacturonate


organism: Dolichospermum flos-aquae


organism: Streptococcus pneumoniae

substrate_name: UDP-D-glucuronate

substrate: UDP-D-glucuronate


proof_lines: pH and temperature not specified in the publication

proof_lines: anti-enantiomer, pH not specified in the publication, temperature not specified in the publication


organism: Mycobacterium tuberculosis

substrate_name: (R)-pristanoyl-CoA

substrate: (R)-pristanoyl-CoA


proof_lines: recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C

proof_lines: recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C


substrate_name: trihydroxycoprostanoyl-CoA

substrate: trihydroxycoprostanoyl-CoA


enzyme_display_name: D-ribulokinase

substrate_name: D-ribulose

enzyme: D-ribulokinase

substrate: D-ribulose


organism: Saccharomyces cerevisiae


proof_lines: recombinant AtUGlcAE1, position 1-64 is lacking


organism: Klebsiella aerogenes

substrate_name: D-arabitol

assay_conditions_summary: pH 7.8; 301.15 K; cofactor ATP

substrate: D-arabitol


enzyme_display_name: fatty-acid peroxygenase

organism: Bacillus subtilis

substrate_name: cumene hydroperoxide

assay_conditions_summary: pH 6.7; 298.15 K; buffer 0.1 M potassium phosphate

proof_lines: mutant enzyme R242A, using myristic acid as cosubstrate, in 0.1 M potassium phosphate buffer (pH 6.7), at 25°C

enzyme: fatty-acid peroxygenase

substrate: cumene hydroperoxide


proof_lines: mutant enzyme R242K, using myristic acid as cosubstrate, in 0.1 M potassium phosphate buffer (pH 6.7), at 25°C


proof_lines: wild type enzyme, using myristic acid as cosubstrate, in 0.1 M potassium phosphate buffer (pH 6.7), at 25°C


organism: Sphingomonas paucimobilis

substrate_name: myristic acid

substrate: myristic acid


organism: Hyphomicrobium sulfonivorans

substrate_name: Dimethyl sulfide

substrate: Dimethyl sulfide


organism: Arthrobacter globiformis

substrate_name: FMN

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADH

substrate: FMN


enzyme_display_name: sphingolipid 4-desaturase

substrate_name: Unknown

enzyme: sphingolipid 4-desaturase

substrate: Unknown


substrate_name: NADH

assay_conditions_summary: pH 7.4; 310.15 K; cofactor NADH

proof_lines: with N-octanoyl-D-erythro-C18-sphinganine as cosubstrate, at pH 7.4 and 37°C

substrate: NADH


enzyme_display_name: formate dehydrogenase (acceptor)

organism: Escherichia coli

substrate_name: formate

enzyme: formate dehydrogenase (acceptor)

substrate: formate


proof_lines: pH 7.5, 24°C, mutant form of the enzyme in which cysteine replaces selenocysteine


organism: Prototheca zopfii

substrate_name: Malonate semialdehyde; CoA; NAD+

assay_conditions_summary: cofactor NAD+

substrate: Malonate semialdehyde; CoA; NAD+


substrate_name: Coenzyme A; NAD+; 3-Oxopropanoate

substrate: Coenzyme A; NAD+; 3-Oxopropanoate


organism: Pseudomonas sp.


organism: Rattus norvegicus

substrate_name: CoA

substrate: CoA


enzyme_display_name: glutamate racemase

organism: Bacillus anthracis

proof_lines: potent competitive inhibitor for RacE2 mutant V149A

enzyme: glutamate racemase


substrate_name: D-glutamate

assay_conditions_summary: pH 9.2; 298.15 K; cofactor NAD+

proof_lines: recombinant wild type enzyme, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml d

proof_lines: recombinant wild type enzyme, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol

proof_lines: recombinant wild type enzyme, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/mL d

substrate: D-glutamate


proof_lines: RacE2 mutant enzyme S207A, pH and temperature not specified in the publication


substrate_name: L-glutamate

substrate: L-glutamate


organism: Streptomyces davaonensis

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


enzyme_display_name: salutaridinol 7-O-acetyltransferase

organism: Papaver somniferum

substrate_name: nudaurine

assay_conditions_summary: cofactor CoA

proof_lines:
with salutaridinol as substrate
with nudaurine as substrate

enzyme: salutaridinol 7-O-acetyltransferase

substrate: nudaurine


enzyme_display_name: plant seed peroxygenase

organism: Avena sativa

enzyme: plant seed peroxygenase


organism: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)


enzyme_display_name: type II protein arginine methyltransferase

organism: Caenorhabditis elegans

substrate_name: histone H4 (at 37 degrees Celsius)

substrate: histone H4 (at 37 degrees Celsius)


substrate_name: salutaridinol

substrate: salutaridinol


enzyme_display_name: diaminobutyrate acetyltransferase

organism: Methylotuvimicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z)

enzyme: diaminobutyrate acetyltransferase


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: UDP-glucose

substrate: UDP-glucose


substrate_name: UDP-Glc (in the presence of polyanion)

substrate: UDP-Glc (in the presence of polyanion)


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: D-glucose 6-phosphate

substrate: D-glucose 6-phosphate


organism: Streptomyces davaonensis (strain DSM 101723 / JCM 4913 / KCC S-0913 / 768)

substrate_name: AF

proof_lines: kcat is 22.0 min(-1) with AF as substrate. kcat is 14.8 min(-1) with S-adenosyl-L-methionine as substrate.

substrate: AF


enzyme_display_name: 4-hydroxybenzoate polyprenyltransferase

substrate_name: farnesyl diphosphate

enzyme: 4-hydroxybenzoate polyprenyltransferase

substrate: farnesyl diphosphate


organism: Oryza sativa

substrate_name: geranyl diphosphate

substrate: geranyl diphosphate


organism: Pseudomonas putida

substrate_name: nonaprenyl diphosphate

substrate: nonaprenyl diphosphate


enzyme_display_name: galactan 1,3-beta-galactosidase

organism: Irpex lacteus

enzyme: galactan 1,3-beta-galactosidase


enzyme_display_name: ([Pyruvate, phosphate dikinase] phosphate) phosphotransferase

organism: Zea mays

substrate_name: phosphate

proof_lines: phosphorylated at a Thr residue, pH 8.3, temperature not specified in the publication

substrate: phosphate


organism: Bifidobacterium longum

substrate_name: H2O; Arabinogalactan

substrate: H2O; Arabinogalactan


organism: Raphanus sativus


enzyme_display_name: gingipain K

organism: Porphyromonas gingivalis

substrate_name: hemoglobin

proof_lines:
pH 7.6, 37°C, human serum hemoglobin
pH 8.5, 37°C

enzyme: gingipain K

substrate: hemoglobin


organism: Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)

substrate_name: pABA (at 70 degrees Celsius)

substrate: pABA (at 70 degrees Celsius)


organism: Escherichia coli (strain K12)

substrate_name: GPP

substrate: GPP


organism: Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)


enzyme_display_name: succinylornithine transaminase

organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

substrate_name: N(2)-succinyl-L-ornithine (at 15 mM 2-ketoglutarate and pH 9.0)

assay_conditions_summary: pH 9

enzyme: succinylornithine transaminase


organism: Homo sapiens

substrate_name: D-ribulose

substrate: D-ribulose


organism: Arabidopsis thaliana

substrate_name: ADP (at pH 6.8)

assay_conditions_summary: pH 6.8; cofactor ADP

enzyme: ([Pyruvate, phosphate dikinase] phosphate) phosphotransferase

substrate: ADP (at pH 6.8)


substrate_name: ADP

assay_conditions_summary: cofactor ADP

substrate: ADP


enzyme_display_name: phosphatidate cytidylyltransferase

enzyme: phosphatidate cytidylyltransferase


organism: Shigella sp. CD2

substrate_name: myo-inositol hexakisphosphate

substrate: myo-inositol hexakisphosphate


proof_lines: pH 5.5, 37°C, recombinant glycosylated enzyme expressed from Pichia pastoris

proof_lines: pH 5.5, 37°C, recombinant non-glycosylated enzyme expressed from Escherichia coli


enzyme_display_name: adenylate cyclase

organism: Bacillus anthracis

substrate_name: ATP

assay_conditions_summary: pH 8; 298.15 K; cofactor ATP

proof_lines:
mutant H351F, pH 8.0, 25°C
mutant H351F

enzyme: adenylate cyclase

substrate: ATP


proof_lines:
wild type, pH 8.0, 25°C
wild type


proof_lines:
mutant H351N, pH 8.0, 25°C
mutant H351N


enzyme_display_name: glucan endo-1,3-alpha-glucosidase

organism: Niallia circulans

proof_lines: recombinant wild-type enzyme, pH 6.5, 30°C

enzyme: glucan endo-1,3-alpha-glucosidase


proof_lines: recombinant mutant AglDELTADS1, pH 6.5, 30°C

proof_lines: recombinant mutant AglDELTADS1CB6DS2, pH 6.5, 30°C

proof_lines: recombinant mutant AglDELTADS1CB6, pH 6.5, 30°C


organism: Lilium longiflorum

proof_lines:
in presence of Ca2+
37°C, pH 8.0
37°C, pH 7.0


organism: Sporolactobacillus laevolacticus

substrate_name: Na-phytate

substrate: Na-phytate


organism: Pseudomonas sp.

substrate_name: limit glucan

substrate: limit glucan


organism: Streptomyces chartreusis

substrate_name: Glucan

substrate: Glucan


organism: Trichoderma viride

substrate_name: pseudonigeran

substrate: pseudonigeran


organism: Pseudomonas aeruginosa

substrate_name: isochorismate

proof_lines: i.e. 2-amino-2-deoxy-isochorismate, pH 6.5

substrate: isochorismate


proof_lines: kcat is 7.3 sec(-1) with 2-amino-2-deoxyisochorismate as substrate. kcat is 1.2 sec(-1) with chorismate as substrate. kcat is 0.2 sec(-1) with isochorismate as substrate. kcat is 0.02 sec(-1) with 4-amino-4-deoxychorismate as substrate.


organism: Thermotoga maritima


proof_lines:
25°C, pH 7.5, wild-type enzyme
37°C, pH 7.5, wild-type enzyme
80°C, pH 7.5, wild-type enzyme


organism: Escherichia coli


organism: Streptomyces coelicolor


enzyme_display_name: V-cath endopeptidase

substrate_name: Z-Phe-Arg 7-amido-4-methylcoumarin

enzyme: V-cath endopeptidase

substrate: Z-Phe-Arg 7-amido-4-methylcoumarin


organism: Helicobacter pylori


substrate_name: Polyphosphate

substrate: Polyphosphate


enzyme_display_name: formylmethionine deformylase

organism: Rattus norvegicus

substrate_name: H2O; N-Formyl-L-methionine

proof_lines:
enzyme from liver
enzyme from spleen
enzyme from brain, heart or muscle

enzyme: formylmethionine deformylase

substrate: H2O; N-Formyl-L-methionine


organism: Euglena gracilis

substrate_name: N-formyl-methionine

substrate: N-formyl-methionine


organism: Escherichia coli (strain K12)

substrate_name: pppGpp

proof_lines: kcat is 0.023 sec(-1) for pppGpp hydrolase activity. kcat is 1.1 sec(-1) for the exopolyphosphatase activity.

substrate: pppGpp


enzyme_display_name: UDP-glucose 4,6-dehydratase

organism: Trichomonas vaginalis

substrate_name: dTDP-D-glucose

enzyme: UDP-glucose 4,6-dehydratase

substrate: dTDP-D-glucose


organism: Acanthamoeba polyphaga mimivirus

substrate_name: UDP-alpha-D-glucose

proof_lines: kcat is 0.27 sec(-1) for UDP-alpha-D-glucose (PubMed:32083779). kcat is 0.18 sec(-1) for dTDP-alpha-D-glucose (PubMed:32083779).

substrate: UDP-alpha-D-glucose


organism: Arabidopsis thaliana

substrate_name: UDP-glucose

proof_lines: kcat is 2860 sec(-1) with UDP-glucose as substrate.

substrate: UDP-glucose


substrate_name: UDP-D-glucose

proof_lines: recombinant RHM2-N protein (amino acids 1–370)

substrate: UDP-D-glucose


organism: Chlorovirus heliozoae


organism: Silene dioica


enzyme_display_name: melilotate 3-monooxygenase

organism: Arthrobacter sp.

substrate_name: melilotate

assay_conditions_summary: cofactor NADH

enzyme: melilotate 3-monooxygenase

substrate: melilotate


organism: Unknown


organism: Bacillus anthracis

substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


organism: Pseudomonas sp.


mutation_signature: stopped flow stopped flow


enzyme_display_name: 4-methylthio 2-oxobutanoate reductase (NADH)

organism: Homo sapiens

substrate_name: oxaloacetate

substrate: oxaloacetate


enzyme_display_name: arginine--pyruvate transaminase

organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

substrate_name: pyruvate

enzyme: arginine--pyruvate transaminase

substrate: pyruvate


organism: Pseudomonas aeruginosa

assay_conditions_summary: pH 9; 310.15 K; cofactor NAD+

proof_lines: calculated, pH 9.0, 37°C, coupled reaction with NAD+ and L-alanine dehydrogenase


organism: Thermus thermophilus

substrate_name: tRNAPhe containing uridine at position 54

proof_lines: pH not specified in the publication, 60°C


enzyme_display_name: HECT-type E3 ubiquitin transferase

enzyme: HECT-type E3 ubiquitin transferase


substrate_name: adenosine 5'-phosphosulfate

proof_lines: cosubstrate adenosine 5-phosphorofluoridate, pH 10.6, 30°C

substrate: adenosine 5'-phosphosulfate


organism: Auxenochlorella pyrenoidosa

substrate_name: ammonia

substrate: ammonia


organism: Chlorella sp.


substrate_name: BCKDHA

proof_lines: kcat is 17.2 min(-1) for BCKDHA. kcat is 43.2 sec(-1) for p-nitrophenylphosphate.

substrate: BCKDHA


organism: Bos taurus

substrate_name: Unknown

substrate: Unknown


substrate_name: 4-nitrophenyl phosphate

proof_lines: mutant N94K, presence of Mg2+, 37°C, pH 7.5

proof_lines: mutant N94K, presence of Mn2+, 30°C, pH 7.5

substrate: 4-nitrophenyl phosphate


proof_lines: wild-type, presence of Mg2+, 37°C, pH 7.5

proof_lines: wild-type, presence of Mn2+, 30°C, pH 7.5


enzyme_display_name: thiosulfate--dithiol sulfurtransferase

organism: Scenedesmus fuscus

substrate_name: dithioerythritol

proof_lines: pH 9, 37°C, with dithioerythritol as second substrate

proof_lines: pH 9, 37°C, with thiosulfate as second substrate, isoenzyme TSR II

enzyme: thiosulfate--dithiol sulfurtransferase

substrate: dithioerythritol


organism: Phormidium uncinatum

substrate_name: thiosulfate

proof_lines: with dithioerythritol as second substrate

substrate: thiosulfate


organism: thermophilic iron-oxidizing bacterium strain TI-1

assay_conditions_summary: pH 6; 318.15 K; cofactor NADPH


organism: Phanerodontia chrysosporium

substrate_name: dithiothreitol

substrate: dithiothreitol


enzyme_display_name: C-terminal processing peptidase

organism: Escherichia coli

enzyme: C-terminal processing peptidase


proof_lines:
wild-type enzyme, mutants V229Q, V229E
wild-type enzyme


substrate_name: Ac-WARAAARAAARBAAB

substrate: Ac-WARAAARAAARBAAB


organism: Spinacia oleracea

substrate_name: C-terminal 19 amino acids of preD1 protein


enzyme_display_name: calmodulin-lysine N-methyltransferase

organism: Ovis aries

substrate_name: VU-1 calmodulin

enzyme: calmodulin-lysine N-methyltransferase

substrate: VU-1 calmodulin


enzyme_display_name: rhamnogalacturonan galacturonohydrolase

organism: Aspergillus aculeatus

enzyme: rhamnogalacturonan galacturonohydrolase


enzyme_display_name: indoxyl-UDPG glucosyltransferase

organism: Persicaria tinctoria

substrate_name: UDP-glucose

enzyme: indoxyl-UDPG glucosyltransferase


organism: Strobilanthes cusia


enzyme_display_name: 1,4-beta-D-xylan synthase

organism: Acer pseudoplatanus

substrate_name: UDP-D-xylose

enzyme: 1,4-beta-D-xylan synthase

substrate: UDP-D-xylose


organism: Arabidopsis thaliana

proof_lines: microsomal fractions of stems from wild-type plants


organism: Hordeum vulgare

substrate_name: pyridylaminated beta-(1,4)-xylotriose

substrate: pyridylaminated beta-(1,4)-xylotriose


enzyme_display_name: medium-chain-enoyl-CoA hydratase

organism: Unknown

enzyme: medium-chain-enoyl-CoA hydratase


organism: Populus trichocarpa

proof_lines: co-substrate: xylotetraose, Km determined in microsomes from poplar stems. pH and temperature not specified in the publication

proof_lines: co-substrate: UDP-D-xylose, Km determined in microsomes from poplar stems. pH and temperature not specified in the publication

proof_lines: co-substrate: xylotetraose, Km determined in microsomes co-expressing PtrGT43B and PtrGT43C. pH and temperature not specified in the publication


organism: Rattus norvegicus

proof_lines: irreversible inhibition, covalent modification shown by peptide mass spectrometry, catalytic residue Glu47 covalently labelled by the inhibitor


organism: Sus scrofa

assay_conditions_summary: 298.15 K; cofactor CoA


enzyme_display_name: ATP-polyphosphate phosphotransferase

organism: Corynebacterium glutamicum

substrate_name: Hexametaphosphate

enzyme: ATP-polyphosphate phosphotransferase

substrate: Hexametaphosphate


organism: Cytophaga hutchinsonii

substrate_name: AMP

substrate: AMP


substrate_name: ATP

assay_conditions_summary: pH 8; 303.15 K; cofactor ATP

substrate: ATP


substrate_name: dADP

substrate: dADP


substrate_name: ADP

assay_conditions_summary: pH 8; 303.15 K; cofactor ADP

substrate: ADP


substrate_name: GDP

substrate: GDP


substrate_name: GMP

substrate: GMP


proof_lines:
pH 8.0, 30°C
pH 8.0, 30°C, cosubstrate: ADP


substrate_name: dAMP

substrate: dAMP


organism: Deinococcus radiodurans


proof_lines:
pH 8.0, 30°C
pH 8.0, 30°C, cosubstrate: ADP
Raw SKiD Env: Temp=30°C, pH=8


organism: Delftia tsuruhatensis

assay_conditions_summary: pH 7; 310.15 K; cofactor ADP


organism: Escherichia coli

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP


substrate_name: ADP; Polyphosphate

substrate: ADP; Polyphosphate


organism: Francisella tularensis subsp. tularensis

assay_conditions_summary: pH 8; 310.15 K; cofactor ADP


assay_conditions_summary: pH 8; 310.15 K; cofactor ATP

proof_lines:
pH 8.0, 37°C
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=37°C, pH=8


substrate_name: GTP

substrate: GTP


assay_conditions_summary: cofactor ATP

proof_lines: mutant enzyme K66A, pH and temperature not specified in the publication

proof_lines: mutant K66A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant enzyme D192A, pH and temperature not specified in the publication

proof_lines: mutant D192A, pH not specified in the publication, temperature not specified in the publication


proof_lines: wild-type enzyme, pH and temperature not specified in the publication

proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant D62A, pH not specified in the publication, temperature not specified in the publication

proof_lines: mutant enzyme D62A, pH and temperature not specified in the publication


proof_lines: mutant enzyme R178A, pH and temperature not specified in the publication

proof_lines: mutant R178A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant D117N, pH not specified in the publication, temperature not specified in the publication

proof_lines: mutant enzyme D117N, pH and temperature not specified in the publication


proof_lines: mutant enzyme R118A, pH and temperature not specified in the publication

proof_lines: mutant R118A, pH not specified in the publication, temperature not specified in the publication


organism: Meiothermus ruber

proof_lines: mutant enzyme N121D, pH and temperature not specified in the publication


proof_lines: mutant N121D, pH not specified in the publication, temperature not specified in the publication


organism: Mycobacterium tuberculosis

assay_conditions_summary: pH 8.5; 298.15 K; cofactor ATP

proof_lines: polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C

proof_lines: polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C


organism: Myxococcus xanthus


organism: Paenarthrobacter aurescens


proof_lines:
pH 8.0, 30°C
pH 8.0, 30°C, cosubstrate: ADP
Raw SKiD Env: Temp=30°C, pH=8


organism: Sinorhizobium meliloti


proof_lines: pH 8.0, 30°C, mutant enzyme H102K/A106E/V115T


organism: Pseudomonas aeruginosa

assay_conditions_summary: pH 7.2; 310.15 K; cofactor ATP


organism: Rhodocyclus sp.

assay_conditions_summary: pH 7.2; cofactor ATP

proof_lines: pH 7.2, room temperature, recombinant polyphosphate kinase


organism: Ruegeria pomeroyi


proof_lines: pH 8.0, 30°C, recombinant isozyme PPK2-2


substrate_name: UDP

substrate: UDP


proof_lines: pH 8.0, 30°C, recombinant isozyme PPK2-1


proof_lines: pH 8.0, 30°C, recombinant isozyme PPK2-3


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

proof_lines: kcat is 57.02 sec(-1) for hydrolase activity with polyP(75) as substrate. kcat is 53.03 sec(-1) for hydrolase activity with polyP(65) as substrate. kcat is 41.23 sec(-1) for hydrolase activity with polyP(45) as substrate. kcat is 40.20 sec(


assay_conditions_summary: pH 7; 310.15 K; cofactor ATP

proof_lines: isoform PPK2, pH and temperature not specified in the publication

proof_lines: isoform PPK1, pH and temperature not specified in the publication

proof_lines: in 50 mM Tris-HCl pH-7.5, 10 mM NH4SO4,10 mM KPO4, pH 7.0, 4 mM MgCl2, 50 mM NaCl, at 37°C


proof_lines: mutant H480A of isoform PPK1, pH and temperature not specified in the publication


proof_lines: mutant H510Q of isoform PPK1, pH and temperature not specified in the publication


proof_lines: mutant H480Q of isoform PPK1, pH and temperature not specified in the publication


substrate_name: (Phosphate)n

substrate: (Phosphate)n


assay_conditions_summary: pH 7.2; 303.15 K; cofactor ATP

proof_lines: pH 7.2, 30°C, recombinant wild-type enzyme


proof_lines: mutant Y524A of isoform PPK1, pH and temperature not specified in the publication


proof_lines: mutant H510A of isoform PPK1, pH and temperature not specified in the publication


mutation_signature: truncated enzyme


organism: Salmonella enterica subsp. enterica serovar Minnesota

assay_conditions_summary: pH 8; 298.15 K; cofactor ATP


organism: Sinomonas atrocyanea

assay_conditions_summary: pH 7; 303.15 K; cofactor ATP

proof_lines:
pH 7.0, 30°C
ATP in form of MnATP2-


organism: Sulfolobus acidocaldarius

assay_conditions_summary: pH 7; 348.15 K; cofactor ATP

proof_lines:
pH 7.0, 75°C
pH 7.0, 75°C, glycogen-complexed enzyme


organism: Burkholderia cepacia

assay_conditions_summary: pH 7; 293.15 K; cofactor ATP

proof_lines: pH 7.0, 20°C, polyphosphate kinase activity in cell extracts, detrmined with Robinson and Wood method

proof_lines: pH 7.0, 20°C, polyphosphate kinase activity in cell extracts, kinetic analysis


proof_lines: pH 7.5, 37°C, guanosine 5-tetraphosphate synthesis


organism: Mycolicibacterium smegmatis

substrate_name: Polyphosphate

assay_conditions_summary: pH 6; 310.15 K; cofactor ATP

substrate: Polyphosphate


organism: Neisseria meningitidis

substrate_name: phosphate

substrate: phosphate


enzyme_display_name: cyclomaltodextrinase

organism: Anoxybacillus flavithermus subsp. flavithermus

substrate_name: beta-cyclodextrin

enzyme: cyclomaltodextrinase

substrate: beta-cyclodextrin


substrate_name: gamma-cyclodextrin

substrate: gamma-cyclodextrin


organism: Evansella clarkii

substrate_name: maltooctaose

substrate: maltooctaose


organism: Flavobacterium sp. 92

substrate_name: alpha-cyclodextrin

substrate: alpha-cyclodextrin


organism: Geobacillus thermopakistaniensis


organism: Massilia timonae


organism: Pyrococcus furiosus

substrate_name: maltoheptaose

substrate: maltoheptaose


enzyme_display_name: serine racemase

organism: Homo sapiens

substrate_name: L-serine

enzyme: serine racemase

substrate: L-serine


proof_lines: pH 8.0, 37°C, recombinant wild-type enzyme


proof_lines: pH and temperature not specified in the publication


organism: Magallana gigas

proof_lines: pH 8.0, 30°C, recombinant mutant P150S/P151S/Y152S


proof_lines: pH 8.0, 30°C, recombinant wild-type enzyme


organism: Mus musculus

proof_lines: pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction

proof_lines: pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction


organism: Cicer arietinum

assay_conditions_summary: pH 7.5; 310.15 K; buffer HEPES


substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


organism: Drosophila melanogaster

substrate_name: KASA(iso-D)LAKY

substrate: KASA(iso-D)LAKY


organism: Sus scrofa


organism: Anoxybacillus flavithermus

substrate_name: soluble starch

substrate: soluble starch


organism: Caenorhabditis elegans


organism: Crassostrea gigas


substrate_name: D-serine

substrate: D-serine


organism: Dictyostelium discoideum


substrate_name: L-Serine

substrate: L-Serine


proof_lines: pH 8.0, 30°C, recombinant mutant P151S/N152S


organism: Penaeus monodon

proof_lines: pH 8.0, 30°C, recombinant mutant H150S/P151S/F152S


organism: Bacillus sp

substrate_name: alpha-cyclodextrin (at pH 7.0 and 50 degrees Celsius)

sequence_source: selected-batch source_record canonical-sequence context for UniProt Q59226

proof_lines: kcat is 78.18 sec(-1) with beta-cyclodextrin as substrate. kcat is 22.11 sec(-1) with amylose as substrate. kcat is 50.36 sec(-1) with amylopectin as substrate (PubMed:16536613). kcat is 101 min(-1) for dimeric form of the enzyme with beta-


sequence_source: selected-batch source_record canonical-sequence context for UniProt A0A7U9P668

proof_lines: kcat is 2739 sec(-1) with alpha-cyclodextrin as substrate. kcat is 1678 sec(-1) with beta-cyclodextrin as substrate. kcat is 821 sec(-1) with gamma-cyclodextrin as substrate. kcat value calculations are based on the dimeric enzyme.


organism: Rattus norvegicus

proof_lines:
cytosolic isozyme
recombinant protein
brush-border membrane isozyme


organism: Unknown


organism: Thermotoga neapolitana

substrate_name: p-nitrophenyl-D-maltopentaoside

proof_lines:
isoenzyme ag1B*
isoenzyme ag1B(1Met)
isoenzyme ag1B(2Met)

substrate: p-nitrophenyl-D-maltopentaoside


substrate_name: Unknown

substrate: Unknown


verification_method: compact batch and family-context review with direct UniProt accession lookup


organism: Pyrococcus horikoshii


organism: Roseobacter litoralis


proof_lines: pH 8.0, 30°C, recombinant His-tagged enzyme


proof_lines: pH 8.0, 30°C, recombinant mutant H150S/N152S


proof_lines: pH 8.0, 30°C, recombinant mutant H150S/P151S/N152S


proof_lines: pH 8.0, 30°C, recombinant mutant H150S/P151S


organism: Acropora millepora


proof_lines: pH 8.0, 30°C, recombinant mutant P150S/P151S/F152S


proof_lines: pH 8.0, 30°C, recombinant mutant P151S/F152S


organism: Escherichia coli


organism: Salmonella enterica


organism: Clostridioides difficile

proof_lines: pH 7.5, 37°C, recombinant wild-type enzyme


organism: Mus musculus

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

proof_lines: mutant Q155D, racemization, pH 7.4, 37°C

proof_lines: mutant Q155D, presence of ATP, pH 7.4, 37°C

proof_lines: mutant Q155D, racemization, presence of ATP, pH 7.4, 37°C


proof_lines: wild-type, racemization, presence of ATP, pH 7.4, 37°C


organism: Oryza sativa

substrate_name: VYP-(L-iso-Asp)-HA

proof_lines:
isoform PIMT1, at pH 8.0 and 50°C
isoform PIMT1, at pH 8.0 and 45°C

substrate: VYP-(L-iso-Asp)-HA


organism: Thermoactinomyces vulgaris

substrate_name: starch

substrate: starch


proof_lines: mutant enzyme E219A/D225A, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication

proof_lines: mutant enzyme E219A/D225A, in the absence of Mg2+, pH and temperature not specified in the publication


proof_lines: wild type enzyme, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication

proof_lines: wild type enzyme, in the absence of Mg2+, pH and temperature not specified in the publication


organism: Arabidopsis thaliana

substrate_name: VYP-L-isoAsp-HA

proof_lines:
native enzyme
at pH 7.5 and 25°C

substrate: VYP-L-isoAsp-HA


organism: Bos taurus

substrate_name: isoACTH

substrate: isoACTH


organism: Cicer arietinum

substrate_name: Val-Tyr-Pro(L-iso-aspartate)-His-Ala

substrate: Val-Tyr-Pro(L-iso-aspartate)-His-Ala


organism: Cucumis sativus

substrate_name: VYP-L-isoaspartyl-HA

assay_conditions_summary: pH 6; 318.15 K; buffer sodium citrate

proof_lines: in sodium citrate buffer at pH 6.0 and 45°C

substrate: VYP-L-isoaspartyl-HA


organism: Cyberlindnera jadinii

substrate_name: delta sleep inducing peptide L-isoaspartate


organism: Daucus carota


substrate_name: VYP (isoD) HA

substrate: VYP (isoD) HA


organism: Triticum aestivum


organism: Zea mays

proof_lines:
enzyme from seeds
enzyme from leaves


organism: Homo sapiens

substrate_name: ovalbumin

proof_lines: in 0.1 M sodium phosphate (pH 6.8), 1 mM EGTA, 0.004% (w/v) sodium azide, and 0.16% (v/v) Triton X-100, at 37°C

proof_lines: pH not specified in the publication, at 37°C

substrate: ovalbumin


organism: Pyrococcus furiosus


organism: Rhodopseudomonas palustris

substrate_name: VYR-isoD-RR

proof_lines:
isoform RPA0376, at pH 6.4 and 30°C
isoform RPA2838, at pH 6.4 and 30°C

substrate: VYR-isoD-RR


organism: Thermotoga maritima

substrate_name: KASA (isoD) LAKY

substrate: KASA (isoD) LAKY


enzyme_display_name: dolichyl-phosphatase

substrate_name: dolichyl phosphate

enzyme: dolichyl-phosphatase

substrate: dolichyl phosphate


organism: Glycine max


organism: Rattus norvegicus


enzyme_display_name: spheroidene monooxygenase

organism: Rhodobacter capsulatus

substrate_name: spirilloxanthin

proof_lines: pH 7.8, 28°C, formation of 2-ketospririlloxanthin

proof_lines: pH 7.8, 28°C, formation of 2,2-diketospririlloxanthin

enzyme: spheroidene monooxygenase

substrate: spirilloxanthin


organism: Rubrivivax gelatinosus

proof_lines: pH 7.8, 28°C, formation of 2,2-dioxospririlloxanthin

proof_lines: pH 7.8, 28°C, formation of 2-oxospririlloxanthin


enzyme_display_name: formate dehydrogenase (coenzyme F420)

organism: Methanobacterium formicicum

substrate_name: formate

assay_conditions_summary: pH 7.5; cofactor FAD

proof_lines: pH 7.5, temperature not specified in the publication, substrate: protioformate

proof_lines: pH 7.5, temperature not specified in the publication, substrate: deuterioformate

enzyme: formate dehydrogenase (coenzyme F420)

substrate: formate


organism: Methanobrevibacter ruminantium

assay_conditions_summary: pH 8

proof_lines: pH 8.0, temperature not specified in the publication


organism: Sus scrofa

substrate_name: C95-dolichyl phosphate

proof_lines: enzyme from grey matter of prenatal subjects

substrate: C95-dolichyl phosphate


organism: Archaeoglobus fulgidus

substrate_name: alpha-cyclodextrin

substrate: alpha-cyclodextrin


organism: Bacillus sp. (in: firmicutes)

substrate_name: amylose

assay_conditions_summary: pH 6

proof_lines:
pH 7.0, dodecamer
pH 7.0
pH 6.0

substrate: amylose


organism: Bacillus subtilis

substrate_name: maltoheptaose

substrate: maltoheptaose


organism: Flavobacterium sp.

substrate_name: beta-cyclodextrin

substrate: beta-cyclodextrin


organism: Geobacillus stearothermophilus

substrate_name: gamma-cyclodextrin

substrate: gamma-cyclodextrin


organism: Heyndrickxia coagulans

substrate_name: maltohexaose

substrate: maltohexaose


organism: Klebsiella oxytoca


organism: Laceyella sacchari


organism: Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)

substrate_name: spheroidene

substrate: spheroidene


substrate_name: coenzyme F420

assay_conditions_summary: cofactor FAD

substrate: coenzyme F420


organism: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

substrate_name: Unknown

substrate: Unknown


organism: Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534)

substrate_name: ATP

assay_conditions_summary: cofactor ATP

substrate: ATP


organism: Dictyostelium discoideum


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)


organism: Trypanosoma brucei brucei (strain 927/4 GUTat10.1)


organism: Trypanosoma cruzi (strain CL Brener)


organism: Arabidopsis thaliana

substrate_name: adenosine 5'-phosphoramidate (at pH 6.8)


organism: Homo sapiens

substrate_name: P(1)-P(3)-bis(5'-adenosyl) triphosphate

substrate: P(1)-P(3)-bis(5'-adenosyl) triphosphate


organism: Lysinibacillus sphaericus


organism: Niallia circulans


organism: Paenibacillus macerans


organism: Paenibacillus sp.


organism: Amphimedon queenslandica

substrate_name: D-serine

substrate: D-serine


organism: Anadara broughtonii

substrate_name: L-serine

substrate: L-serine


organism: Anneissia japonica


organism: Aplysia californica


enzyme_display_name: isoflavone 4'-O-methyltransferase

organism: Cicer arietinum

substrate_name: S-adenosyl-L-methionine

proof_lines: pH 8.8, 30°C, versus 7,4-dihydroxyisoflavone

proof_lines: pH 8.8, 30°C, versus S-adenosyl-L-methionine

enzyme: isoflavone 4'-O-methyltransferase

substrate: S-adenosyl-L-methionine


organism: Palaeococcus pacificus


organism: Pyrococcus furiosus

substrate_name: starch

proof_lines:
pH 4.5, 90°C, recombinant enzyme
pH 4.5, 90°C

substrate: starch


organism: unidentified


organism: Solanum lycopersicum


organism: Streptomyces garyphalus


organism: Bombyx mori


assay_conditions_summary: pH 8; 310.15 K; cofactor ATP

proof_lines:
in presence of ATP, pH 8.0, 37°C
pH 8.0
in absence of ATP, pH 8.0, 37°C


organism: Macrostomum lignano


organism: Manihot esculenta


organism: Mus musculus

proof_lines: racemization reaction, isoform A, pH 8.6, 37°C


organism: Pyrobaculum islandicum


organism: Rattus norvegicus


organism: Schizosaccharomyces pombe

proof_lines: pH and temperature not specified in the publication


organism: Escherichia coli

substrate_name: ATPgammaS

substrate: ATPgammaS


organism: Gallus gallus


assay_conditions_summary: pH 7.7; cofactor ATP


enzyme_display_name: limonene dehydrogenase

organism: Castellaniella defragrans

substrate_name: limonene

enzyme: limonene dehydrogenase

substrate: limonene


organism: Xanthobacter autotrophicus


organism: Glycine max


enzyme_display_name: [G-protein-coupled receptor] kinase

organism: Homo sapiens

substrate_name: rhodopsin

proof_lines: recombinant wild type enzyme, at pH 8.0 and 25°C

enzyme: [G-protein-coupled receptor] kinase

substrate: rhodopsin


proof_lines: recombinant mutant enzyme S500A, at pH 8.0 and 25°C


proof_lines: recombinant mutant enzyme T10A, at pH 8.0 and 25°C


organism: Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CCUG 56034 / CIP 105152 / NBRC 16084 / F199)

proof_lines: kcat is 25 min(-1) with 8'-apo-beta-carotenal as substrate. kcat is 8.4 min(-1) with 4'-apo-beta-carotenal as substrate. kcat is 0.2 min(-1) with gamma-carotene as substrate.


organism: Castellaniella defragrans (strain DSM 12143 / CCUG 39792 / 65Phen)

substrate_name: limonene (at 28 degrees Celsius)

substrate: limonene (at 28 degrees Celsius)


organism: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

substrate_name: maltotriose (at pH 6.5 and 85 degrees Celsius)


organism: Thermotoga neapolitana

substrate_name: beta-cyclodextrin (at 80 degrees Celsius)


enzyme_display_name: UDP-glucose 4,6-dehydratase

substrate_name: UDP-alpha-D-glucose

enzyme: UDP-glucose 4,6-dehydratase

substrate: UDP-alpha-D-glucose


organism: Aspergillus phoenicis


organism: Clostridium perfringens


substrate_name: 4-nitrophenyl alpha-D-mannopyranoside

proof_lines: in 100 mM sodium acetate at pH 4.0 and 37°C

proof_lines: in 100 mM sodium acetate (pH 4.0), at 37°C

substrate: 4-nitrophenyl alpha-D-mannopyranoside


enzyme_display_name: plasmalogen synthase

assay_conditions_summary: cofactor CoA

proof_lines:
submandibular gland
sublingual gland

enzyme: plasmalogen synthase


enzyme_display_name: 13-hydroxylupanine O-tigloyltransferase

organism: Lupinus albus

proof_lines:
with 13-alpha-hydroxylupanine
with 13-alpha-hydroxymultiflorine

enzyme: 13-hydroxylupanine O-tigloyltransferase


substrate_name: palmitoyl-CoA (in the presence of 1-palmitoyl-LPC as cosubstrate)


organism: Mus musculus


substrate_name: acetyl-CoA

substrate: acetyl-CoA


substrate_name: 13alpha-hydroxymultiflorine (with tigloyl-CoA as cosubstrate)


proof_lines: kcat is 5.4 min(-1) with alpha-D-Man-(1->3)-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol as substrate. kcat is 63.2 min(-1) with alpha-D-Man-(1->6)-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodo


organism: Oryctolagus cuniculus

substrate_name: tetrasaccharide-diphosphoryl-lipid

substrate: tetrasaccharide-diphosphoryl-lipid


substrate_name: GDP-alpha-D-mannose

substrate: GDP-alpha-D-mannose


substrate_name: N-acetyl-alpha-D-glucosaminyl-diphosphodolichol (at pH 7.5 and 37 degrees Celsius)


organism: Cricetulus griseus


substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


organism: Vigna radiata


enzyme_display_name: exo-chitinase (non-reducing end)

organism: Pyrococcus furiosus

enzyme: exo-chitinase (non-reducing end)


organism: Sulfolobus acidocaldarius

substrate_name: UDP-N-acetyl-alpha-D-glucosamine

substrate: UDP-N-acetyl-alpha-D-glucosamine


organism: Bacillus licheniformis

substrate_name: colloidal chitin

substrate: colloidal chitin


enzyme_display_name: ADP deaminase

organism: Aspergillus melleus

substrate_name: adenosine

enzyme: ADP deaminase

substrate: adenosine


organism: Aspergillus ochraceus

substrate_name: Adenosine diphosphate

substrate: Adenosine diphosphate


enzyme_display_name: propanoyl-CoA:succinate CoA transferase

organism: Escherichia coli

substrate_name: propanoyl-CoA

assay_conditions_summary: pH 7; 298.15 K; cofactor CoA

enzyme: propanoyl-CoA:succinate CoA transferase

substrate: propanoyl-CoA


organism: Propionibacterium freudenreichii subsp. shermanii

substrate_name: acetate

assay_conditions_summary: pH 8; 298.15 K; cofactor CoA

proof_lines: with acetate as cosubstrate, at pH 8.0 and 25°C

substrate: acetate


organism: Escherichia coli (strain K12)

proof_lines: kcat is 0.72 sec(-1) with propanoyl-CoA as substrate.


organism: Salisediminibacterium selenitireducens

substrate_name: beta-D-glucose 1-phosphate

proof_lines:
pH 7.5, 30°C
pH 8.0, 30°C
Raw SKiD Env: Temp=30°C, pH=7.5

substrate: beta-D-glucose 1-phosphate


substrate_name: D-sorbitol 6-phosphate

substrate: D-sorbitol 6-phosphate


organism: Malus domestica


organism: Bacillus selenitireducens (strain ATCC 700615 / DSM 15326 / MLS10)

proof_lines: kcat is 95 sec(-1) with 2-O-alpha-D-glucopyranosyl-sn-glycerol.


substrate_name: fructose-1-P (with magnesium ions as cofactor and at pH 9)

proof_lines: kcat is 3.7 sec(-1) with fructose-1-P as substrate. kcat is 2.7 sec(-1) with ribose-5-P as substrate. kcat is 13 sec(-1) with glucose-6-P as substrate. kcat is 3.1 sec(-1) with fructose-6-P as substrate. kcat is 7.5 sec(-1) with beta-D-gluc


substrate_name: 2-deoxyglucose-6-P (with manganese ions as cofactor and at pH 9)

proof_lines: kcat is 33 sec(-1) with 2-deoxyglucose-6-P as substrate. kcat is 11 sec(-1) with mannose-6-P as substrate. kcat is 9.4 sec(-1) with 2-deoxyribose-5-P as substrate. kcat is 2.8 sec(-1) with ribose-5-P as substrate. kcat is 25 sec(-1) with gl


enzyme_display_name: oxamate carbamoyltransferase

substrate_name: oxamate

proof_lines: kcat is 58.5 sec(-1) with oxamate as substrate. kcat is 35.5 sec(-1) with carbamoyl phosphate as substrate.

enzyme: oxamate carbamoyltransferase

substrate: oxamate


substrate_name: Carbamoyl phosphate

proof_lines: pH 9.0, 30°C, recombinant His-tagged enzyme

substrate: Carbamoyl phosphate


organism: Bacillus subtilis (strain 168)

substrate_name: L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala

proof_lines: kcat is 5.7 sec(-1) with L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala as substrate. kcat is 2.6 sec(-1) with L-Ala-gamma-D-Glu-L-Lys-D-Ala as substrate. kcat is 1.2 sec(-1) with L-Ala-gamma-D-Glu-L-Lys as substrate.

substrate: L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala


organism: Bacillus subtilis

proof_lines: pH and temperature not specified in the publication


enzyme_display_name: indoleacetate decarboxylase

organism: Olsenella uli

proof_lines: mutant enzyme H514A, pH and temperature not specified in the publication

enzyme: indoleacetate decarboxylase


proof_lines: wild type enzyme, pH and temperature not specified in the publication


enzyme_display_name: mannose-6-phosphate 6-reductase

organism: Apium graveolens

substrate_name: D-mannitol 1-phosphate

assay_conditions_summary: cofactor NADPH

proof_lines:
enzyme purified from leaves
recombinant enzyme

enzyme: mannose-6-phosphate 6-reductase

substrate: D-mannitol 1-phosphate


organism: Phelipanche ramosa

substrate_name: D-mannose 6-phosphate

substrate: D-mannose 6-phosphate


organism: Striga hermonthica

substrate_name: NADPH

substrate: NADPH


enzyme_display_name: chrysanthemyl diphosphate synthase

organism: Artemisia spiciformis

substrate_name: dimethylallyl diphosphate

proof_lines: pH not specified in the publication, temperature not specified in the publication

enzyme: chrysanthemyl diphosphate synthase

substrate: dimethylallyl diphosphate


organism: Tanacetum cinerariifolium

proof_lines: pH 7.5, 30°C, His-tagged or detagged recombinant enzyme


organism: Clostridium scatologenes


organism: Tractidigestivibacter scatoligenes


proof_lines: kcat is 0.5 min(-1) with dimethylallyl diphosphate as substrate (PubMed:11287653). kcat is 0.0033 min(-1) with chrysanthemyl diphosphate as substrate (PubMed:25378387).


enzyme_display_name: 6beta-hydroxyhyoscyamine epoxidase

organism: Atropa belladonna

enzyme: 6beta-hydroxyhyoscyamine epoxidase


organism: Brugmansia sanguinea

proof_lines:
SUMO-tagged enzyme, at pH 7.6 and 34°C
untagged enzyme, at pH 7.6 and 34°C


organism: Datura metel


enzyme_display_name: Deleted entry

organism: Rattus norvegicus

substrate_name: H+; NADPH; Aquacob(III)alamin

enzyme: Deleted entry

substrate: H+; NADPH; Aquacob(III)alamin


enzyme_display_name: hydrogen-dependent carbon dioxide reductase

organism: Acetobacterium woodii


organism: Thermoanaerobacter kivui

substrate_name: formate

proof_lines:
at pH 7.0 and 30°C
at pH 7.0 and 60°C

enzyme: hydrogen-dependent carbon dioxide reductase

substrate: formate


proof_lines: variant FdhF_HycB3DELTA159-184 bioelectrode, at pH 6.2 and 25°C


enzyme_display_name: sulfide-cytochrome-c reductase (flavocytochrome c)

organism: Paracoccus pantotrophus

substrate_name: oxidized heart cytochrome c

substrate: oxidized heart cytochrome c


organism: Thiobacillus sp.

substrate_name: Sulfide

enzyme: sulfide-cytochrome-c reductase (flavocytochrome c)

substrate: Sulfide


organism: Anisodus tanguticus


organism: Hyoscyamus niger


organism: Thioalkalivibrio paradoxus

substrate_name: ferricytochrome c

substrate: ferricytochrome c


organism: Arabidopsis thaliana


organism: Gentiana triflora

substrate_name: caffeoyl-CoA

proof_lines: with delphinidin 3,5-diglucoside as cosubstrate

substrate: caffeoyl-CoA


enzyme_display_name: maltokinase

organism: Actinoplanes missouriensis

substrate_name: maltose (at pH 8)

enzyme: maltokinase

substrate: maltose (at pH 8)


organism: Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)

substrate_name: ATP (at 37 degrees Celsius and at pH 8)

substrate: ATP (at 37 degrees Celsius and at pH 8)


enzyme_display_name: Transferred entry: 2.4.3.8

organism: Escherichia coli

enzyme: Transferred entry: 2.4.3.8


organism: Neisseria meningitidis


substrate_name: ATP

assay_conditions_summary: pH 8.5; 295.15 K; cofactor ATP

substrate: ATP


organism: Mycobacterium tuberculosis variant bovis

proof_lines: pH 8.0, 37°C, recombinant His-tagged Mak


organism: Homo sapiens

substrate_name: CMP-N-acetylneuraminate; Fetuin

substrate: CMP-N-acetylneuraminate; Fetuin


substrate_name: CMP-N-acetylneuraminate; Colominate

substrate: CMP-N-acetylneuraminate; Colominate


enzyme_display_name: thymidylate 5'-phosphatase

substrate_name: AMP

enzyme: thymidylate 5'-phosphatase

substrate: AMP


organism: Bacillus subtilis

substrate_name: dTMP

substrate: dTMP


organism: Mus musculus

proof_lines: pH 7.5, 37°C, exponentially growing cells


enzyme_display_name: micrococcal nuclease

organism: Staphylococcus sp.

substrate_name: DNA

enzyme: micrococcal nuclease

substrate: DNA


enzyme_display_name: zinc D-Ala-D-Ala carboxypeptidase

organism: Enterococcus faecalis

substrate_name: D-Ala-D-Ala

proof_lines: determined by isothermal titration calorimetry, at pH 8.0 and 37°C

proof_lines: determined by ninhydrin/Cd(II) assay, at pH 8.0 and 37°C

enzyme: zinc D-Ala-D-Ala carboxypeptidase

substrate: D-Ala-D-Ala


organism: Streptomyces albus G

substrate_name: Unknown

substrate: Unknown


enzyme_display_name: methylphosphonate synthase

organism: Nitrosopumilus maritimus

proof_lines: temperature not specified in the publication, pH not specified in the publication

enzyme: methylphosphonate synthase


enzyme_display_name: phosphoribosyl-ATP diphosphatase

organism: Salmonella enterica subsp. enterica serovar Typhimurium

enzyme: phosphoribosyl-ATP diphosphatase


enzyme_display_name: guanosine-diphosphatase

substrate_name: IDP

enzyme: guanosine-diphosphatase

substrate: IDP


organism: Saccharomyces cerevisiae

substrate_name: GDP

substrate: GDP


enzyme_display_name: phosphoamidase

organism: Oryctolagus cuniculus

enzyme: phosphoamidase


organism: Rattus norvegicus

substrate_name: Phosphoramidate

substrate: Phosphoramidate


enzyme_display_name: CDP-glucose 4,6-dehydratase

substrate_name: CDP-D-glucose

enzyme: CDP-glucose 4,6-dehydratase

substrate: CDP-D-glucose


organism: Yersinia pseudotuberculosis


proof_lines:
wild-type enzyme
wild-type


substrate_name: CMP-acetylneuraminate

substrate: CMP-acetylneuraminate


enzyme_display_name: chlorogenate hydrolase

organism: Aspergillus niger

substrate_name: chlorogenic acid

enzyme: chlorogenate hydrolase

substrate: chlorogenic acid


organism: Bacillus amyloliquefaciens

substrate_name: chlorogenate

substrate: chlorogenate


organism: bacterium


organism: Ustilago maydis

substrate_name: methyl ferulate

substrate: methyl ferulate


substrate_name: methyl caffeate

substrate: methyl caffeate


organism: Acinetobacter baumannii

assay_conditions_summary: pH 7; cofactor ATP

proof_lines: pH 7.0, temperature not specified in the publication

proof_lines: pH 8.0, temperature not specified in the publication

proof_lines: pH 7.5, temperature not specified in the publication


organism: Candida albicans


organism: Homo sapiens


enzyme_display_name: aspulvinone dimethylallyltransferase

organism: Unknown

enzyme: aspulvinone dimethylallyltransferase


organism: Aspergillus terreus

substrate_name: dimethylallyl diphosphate

proof_lines: inhibition against dimethylallyl diphosphate

substrate: dimethylallyl diphosphate


enzyme_display_name: isatin hydrolase

organism: Burkholderia sp. IDO3

substrate_name: isatin

proof_lines: pH and temperature not specified in the publication

enzyme: isatin hydrolase

substrate: isatin


organism: Mesorhizobium loti


organism: Roseibium aggregatum (strain ATCC 25650 / DSM 13394 / JCM 20685 / NBRC 16684 / NCIMB 2208 / IAM 12614 / B1)

proof_lines: kcat is 24 sec(-1) for isatin as substrate.


organism: Ralstonia solanacearum


organism: Roseibium aggregatum


organism: Klebsiella aerogenes

substrate_name: mannitol

proof_lines: carbamoyl phosphate, D-mannose 6-phosphate, pH 7.5, 25°C

substrate: mannitol


enzyme_display_name: terephthalate 1,2-dioxygenase

organism: Comamonas sp.

substrate_name: NADPH

assay_conditions_summary: 303.15 K; cofactor NADPH

enzyme: terephthalate 1,2-dioxygenase

substrate: NADPH


enzyme_display_name: membrane Pro-Xaa carboxypeptidase

organism: Penicillium janthinellum

substrate_name: myoglobin

proof_lines: Michaelis-Menten kinetics in the bulk solution

enzyme: membrane Pro-Xaa carboxypeptidase

substrate: myoglobin


organism: Sus scrofa

substrate_name: Benzyloxycarbonyl-Pro-Met

substrate: Benzyloxycarbonyl-Pro-Met


enzyme_display_name: steroid 22S-hydroxylase

organism: Arabidopsis thaliana

proof_lines: kcat is 1.8 min(-1) for campesterol (at pH 7.25 and 30 degrees Celsius) (PubMed:16460510). kcat is 0.049 min(-1) for campestanol (at pH 7.25 and 30 degrees Celsius) (PubMed:16460510).

enzyme: steroid 22S-hydroxylase


organism: Locusta migratoria


enzyme_display_name: flavone synthase II

organism: Citrus japonica

substrate_name: isosakuranetin

enzyme: flavone synthase II

substrate: isosakuranetin


organism: Ustilago maydis


substrate_name: methyl p-coumarate

substrate: methyl p-coumarate


organism: Berberis koetineana

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


organism: Papaver somniferum

proof_lines: with (R,S)-6-O-methylnorlaudanosoline as substrate

proof_lines: with (R,S)-norlaudanosoline as substrate


enzyme_display_name: phenol O-methyltransferase

organism: Phanerodontia chrysosporium

enzyme: phenol O-methyltransferase


organism: Mycosarcoma maydis


organism: Homo sapiens

substrate_name: Acetaminophen; S-Adenosyl-L-methionine

substrate: Acetaminophen; S-Adenosyl-L-methionine


organism: Glandularia x hybrida

substrate_name: malonyl-CoA

assay_conditions_summary: pH 7; 303.15 K; cofactor CoA

substrate: malonyl-CoA


organism: Lamium purpureum


organism: Petroselinum crispum

substrate_name: kaempferol 3-O-glucoside

substrate: kaempferol 3-O-glucoside


substrate_name: 2-Naphthol glucoside

proof_lines: kcat is 40 sec(-1) for 2-Naphthol glucoside. kcat is 46 sec(-1) for 1-Naphthol glucoside. kcat is 33 sec(-1) for kaempferol 7-O-glucoside. kcat is 120 sec(-1) for 4-nitrophenyl glucoside. kcat is 19 sec(-1) for 4-methylumbelliferone glucosi

substrate: 2-Naphthol glucoside


organism: Nicotiana tabacum

substrate_name: kaempferol 7-O-glucoside (at 30 degrees Celsius)

proof_lines: kcat is 11.0 sec(-1) for kaempferol 7-O-glucoside. kcat is 6.8 sec(-1) for kaempferol 3-O-glucoside. kcat is 6.5 sec(-1) for 2-naphthol glucoside. kcat is 8.0 sec(-1) for malonyl-CoA. kcat is 9.3 sec(-1) for succinyl-CoA.


organism: Rhizoctonia solani

substrate_name: chlorogenate


organism: Oryza sativa

substrate_name: eriodictyol

substrate: eriodictyol


organism: Cavia porcellus


organism: Burkholderia pseudomallei


organism: Aspergillus niger

substrate_name: chlorogenic acid

substrate: chlorogenic acid


organism: Bacillus amyloliquefaciens

substrate: chlorogenate


substrate_name: WcbL; ATP

substrate: WcbL; ATP


organism: Geobacter metallireducens

substrate_name: acetyl-CoA

assay_conditions_summary: pH 6.8; 303.15 K; cofactor CoA

proof_lines: Km above 0.5 mM, with cyclohexane-1-carboxylate as cosubstrate, at pH 6.8 and 30°C

proof_lines: Km above 0.2 mM, with cyclohexane-1-carboxylate as cosubstrate, at pH 6.8 and 30°C

substrate: acetyl-CoA


enzyme_display_name: acetyl-CoA C-myristoyltransferase

organism: Rattus norvegicus

enzyme: acetyl-CoA C-myristoyltransferase


enzyme_display_name: diaminopimelate epimerase

organism: Unknown

substrate_name: meso-diaminoheptanedioate

enzyme: diaminopimelate epimerase

substrate: meso-diaminoheptanedioate


organism: Escherichia coli

substrate_name: LL-diaminoheptanedioate

substrate: LL-diaminoheptanedioate


organism: Mycobacterium tuberculosis

proof_lines: recombinant DapF consisting of silent mutation of the first 10 codons of the open reading frame


organism: Priestia megaterium


organism: Haemophilus influenzae

proof_lines:
forward reaction
Raw SKiD Env: Temp=-----, pH=-----


source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0


organism: Thermotoga maritima


enzyme_display_name: mRNA 5'-phosphatase

organism: Vaccinia virus (strain Western Reserve)

substrate_name: GpppA

proof_lines: A-570 and A-763 mutants have 3-fold and 10-fold higher Km values for GpppA.

enzyme: mRNA 5'-phosphatase

substrate: GpppA


substrate_name: acetoacetyl-CoA

substrate: acetoacetyl-CoA


organism: Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15)

substrate_name: cyclohexane carboxylic acid (with succinyl-CoA as CoA donor)


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: pppA-poly(A)

substrate: pppA-poly(A)


organism: Semliki forest virus

substrate_name: nsP2 RNA triphosphatase activity (at pH 8.0)


organism: Venezuelan equine encephalitis virus (strain 3880)

substrate_name: a 12 residues substrate for nsp2 protease activity


organism: Venezuelan equine encephalitis virus (strain Trinidad donkey)

substrate: a 12 residues substrate for nsp2 protease activity


organism: Rhizobium meliloti (strain 1021)

substrate_name: FMN

assay_conditions_summary: cofactor FMN

proof_lines: kcat is 15 h(-1), 7.2 h(-1) and 7.6 h(-1) for FMN, NAD and NADP, respectively.

substrate: FMN


organism: Sinorhizobium meliloti

substrate_name: NADPH

assay_conditions_summary: pH 7.5; cofactor NADPH

substrate: NADPH


organism: Haloferax volcanii

substrate_name: ATP

assay_conditions_summary: pH 8; 315.15 K; cofactor ATP

substrate: ATP


organism: Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)

substrate_name: KDGal

sequence_source: UniProtKB D4GR05 reviewed-entry scalar metadata

substrate: KDGal


enzyme_display_name: diadenylate cyclase

organism: Bacillus subtilis (strain 168)

assay_conditions_summary: cofactor NADH

enzyme: diadenylate cyclase


organism: Mycolicibacterium smegmatis

assay_conditions_summary: pH 5.4; 310.15 K; cofactor ATP


enzyme_display_name: phenylsuccinyl-CoA transferase

organism: Aromatoleum aromaticum (strain DSM 19018 / LMG 30748 / EbN1)

substrate_name: Unknown

proof_lines: kcat is 0.178 sec(-1) with (R/S)-phenylsuccinate as substrate. kcat is 0.092 sec(-1) with (R)-benzylsuccinate as substrate. kcat is 0.064 sec(-1) with (S)-benzylsuccinate as substrate. kcat is 0.078 sec(-1) with (R/S)-benzylsuccinate as sub

enzyme: phenylsuccinyl-CoA transferase

substrate: Unknown


organism: Aromatoleum aromaticum

substrate_name: (R/S)-phenylsuccinyl-CoA

assay_conditions_summary: pH 8; cofactor CoA

proof_lines: at pH 8.0, temperature not specified in the publication

substrate: (R/S)-phenylsuccinyl-CoA


enzyme_display_name: endo-chitodextinase

organism: Vibrio furnissii

enzyme: endo-chitodextinase


enzyme_display_name: glucosylglycerate hydrolase

organism: Rubrobacter radiotolerans

enzyme: glucosylglycerate hydrolase


organism: Selaginella moellendorffii

sequence_source: UniProtKB:D8QTR2 (direct accession lookup; live reviewed entry with sequence present)


organism: Thermus thermophilus

sequence_source: UniProtKB:Q72K04 (direct accession lookup; live reviewed entry with sequence present)


enzyme_display_name: 4-nitrophenol 2-monooxygenase

organism: Oryctolagus cuniculus

assay_conditions_summary: pH 7.6; cofactor NADPH

proof_lines: reconstituted system of purified isozyme 3a, dilaroylglyceryl-3-phosphorylcholine and NADPH-cytochrome P-450 reductase at pH 7.6

enzyme: 4-nitrophenol 2-monooxygenase


organism: Homo sapiens

proof_lines: inhibition of chlorzoxazone 6-hydroxylation

proof_lines: inhibition of 4-nitrophenol hydroxylation


organism: Nocardia sp.


enzyme_display_name: desampylase

organism: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

substrate_name: SAMP2 dimer (with PfJAMM1 in the dimeric form, at 100 degrees Celsius)

proof_lines: kcat is 0.28 min(-1) for the cleavage of SAMP2 dimer using PfJAMM1 in the dimeric form. kcat is 0.16 min(-1) for the cleavage of SAMP2 dimer using PfJAMM1 in the monomeric form (at 100 degrees Celsius). kcat is 0.0016 min(-1) for the cleava

enzyme: desampylase


organism: Pyrococcus furiosus

substrate_name: SAMP2 dimer; H2O

substrate: SAMP2 dimer; H2O


enzyme_display_name: brachyurin

organism: Gadus morhua

enzyme: brachyurin


organism: Leptuca pugilator

substrate_name: N-benzoyl-L-Arg-p-nitroanilide

substrate: N-benzoyl-L-Arg-p-nitroanilide


enzyme_display_name: dTDP-epi-vancosaminyltransferase

organism: Amycolatopsis orientalis

substrate_name: desvancosaminyl-vancomycin

proof_lines:
pH 7.5, 37°C
Raw SKiD Env: Temp=37°C, pH=7.5

enzyme: dTDP-epi-vancosaminyltransferase

substrate: desvancosaminyl-vancomycin


enzyme_display_name: tRNA-guanine(15) transglycosylase

organism: Methanosarcina acetivorans

substrate_name: guanine15 in tRNA

proof_lines:
recombinant enzyme, at pH 7.5 and 37°C
native enzyme, at pH 7.5 and 37°C

enzyme: tRNA-guanine(15) transglycosylase

substrate: guanine15 in tRNA


enzyme_display_name: fatty acid photodecarboxylase

organism: Chlorella variabilis

substrate_name: palmitate

enzyme: fatty acid photodecarboxylase

substrate: palmitate


substrate_name: elaidic acid

substrate: elaidic acid


mutation_signature: truncated enzyme form FAP2 / truncated enzyme form FAP2

proof_lines: substrate presented in microemulsion, truncated enzyme form FAP2, at pH 9.0 and 20°C

proof_lines: substrate presented in large unilamellar vesicles, truncated enzyme form FAP2, at pH 9.0 and 20°C


organism: Escherichia coli

substrate_name: D-erythrose 4-phosphate

assay_conditions_summary: pH 7.5; 298.15 K; buffer MOPS; cofactor NAD+

proof_lines: wild type enzyme, in MOPS buffer (50 mM, pH 7.5), 0.050 mM CoCl2, 0.010 mM NAD+, D-erythrose 4-phosphate, 2 mM pyruvate, 1 unit of 3-dehydroquinate synthase, 1 unit of 3-dehydroquinate dehydratase, at 25°C

substrate: D-erythrose 4-phosphate


proof_lines: mutant enzyme F33I/D58N/Q72H/A75V/V85A/V154F, in MOPS buffer (50 mM, pH 7.5), 0.050 mM CoCl2, 0.010 mM NAD+, D-erythrose 4-phosphate, 2 mM pyruvate, 1 unit of 3-dehydroquinate synthase, 1 unit of 3-dehydroquinate dehydratase, at 25°C


organism: Roseateles saccharophilus


substrate_name: tRNA-Asp

substrate: tRNA-Asp


organism: Mycolicibacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 / 3849)

substrate_name: glucosylglycerate (at 37 degrees Celsius, C-terminally tagged variant)


organism: Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828 / KAW 2/3)

substrate_name: KDG (at 60 degrees Celsius and pH 6.2)

substrate: KDG (at 60 degrees Celsius and pH 6.2)


enzyme_display_name: hapalindole-type alkaloid chlorinase

organism: Fischerella ambigua

substrate_name: ambiguine C

proof_lines: pH and temperature not specified in the publication

enzyme: hapalindole-type alkaloid chlorinase

substrate: ambiguine C


organism: Hapalosiphon welwitschii

substrate_name: 12-epi-hapalindole C

substrate: 12-epi-hapalindole C


organism: Penicillium glabrum


enzyme_display_name: nitric oxide reductase (menaquinol)

organism: Pyrobaculum aerophilum

proof_lines:
54.5°C, pH 7.0, purified qNOR
54.5°C, pH 7.0, qNOR in membrane

enzyme: nitric oxide reductase (menaquinol)


organism: Schinkia azotoformans

substrate_name: nitric oxide

proof_lines: using plumbagin as electron donor, pH and temperature not specified in the publication

proof_lines: using cytochrome c551 as electron donor, pH and temperature not specified in the publication

proof_lines: using menaquinol as electron donor, pH and temperature not specified in the publication

substrate: nitric oxide


organism: Methanohalophilus portucalensis

substrate_name: glycine

substrate: glycine


organism: Thalassiosira pseudonana

substrate_name: N,N-dimethylglycine

substrate: N,N-dimethylglycine


proof_lines: truncation mutant carrying the N-terminal part, pH 8.0, 25°C


proof_lines: truncation mutant carrying the C-terminal part, pH 8.0, 25°C


enzyme_display_name: small RNA 2'-O-methyltransferase

organism: Acetivibrio thermocellus

substrate_name: [24mer sRNA]-3'-end ribonucleotide

enzyme: small RNA 2'-O-methyltransferase

substrate: [24mer sRNA]-3'-end ribonucleotide


organism: Arabidopsis thaliana


proof_lines: above 0.02 mM, C-terminal domain, pH 7.5, 37°C


enzyme_display_name: isobutyraldoxime O-methyltransferase

organism: Pseudomonas sp.

enzyme: isobutyraldoxime O-methyltransferase


enzyme_display_name: (phenol)carboxyphthiodiolenone synthase

organism: Mycobacterium tuberculosis

substrate_name: (S)-methylmalonyl-CoA

enzyme: (phenol)carboxyphthiodiolenone synthase

substrate: (S)-methylmalonyl-CoA


enzyme_display_name: lysine N-acetyltransferase

organism: Bos taurus

substrate_name: acetyl phosphate

proof_lines:
cosubstrate L-ornithine
cosubstrate L-lysine

enzyme: lysine N-acetyltransferase

substrate: acetyl phosphate


organism: Salmonella enterica

substrate_name: Acs (AMP forming)

assay_conditions_summary: pH 7; 310.15 K; cofactor CoA

substrate: Acs (AMP forming)


enzyme_display_name: lipopolysaccharide core heptose(I) kinase

organism: Escherichia coli

substrate_name: lipopolysaccharide acceptor

assay_conditions_summary: cofactor ATP

substrate: lipopolysaccharide acceptor


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

substrate_name: ATP

proof_lines: kcat is 27.23 min(-1) for lipopolysaccharide kinase activity.

enzyme: lipopolysaccharide core heptose(I) kinase

substrate: ATP


organism: Escherichia coli (strain K12)

substrate_name: KDPGal

proof_lines: kcat is 4 sec(-1) with KDPGal as substrate.

substrate: KDPGal


assay_conditions_summary: pH 7.5; 308.15 K; cofactor ATP


organism: Pseudomonas aeruginosa

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP


enzyme_display_name: muramoyltetrapeptide carboxypeptidase

organism: Drosophila melanogaster

enzyme: muramoyltetrapeptide carboxypeptidase


organism: Novosphingobium aromaticivorans


substrate_name: UDP-N-acetylmuramyl-Ala-D-Glu-Dap-D-Ala

substrate: UDP-N-acetylmuramyl-Ala-D-Glu-Dap-D-Ala


organism: Lysinibacillus sphaericus


organism: Proteus vulgaris


enzyme_display_name: tropomyosin kinase

organism: Gallus gallus

substrate_name: alpha-tropomyosin

proof_lines: 37°C, pH 7.5, skeletal alpha-tropomyosin

enzyme: tropomyosin kinase

substrate: alpha-tropomyosin


enzyme_display_name: thermopsin

organism: Sulfolobus acidocaldarius

enzyme: thermopsin


enzyme_display_name: beta-porphyranase

organism: Chondrus crispus

substrate_name: Porphyran

enzyme: beta-porphyranase

substrate: Porphyran


organism: Wenyingzhuangia fucanilytica


organism: Phocaeicola plebeius

proof_lines: dockerin fusion chimeric enzyme, pH and temperature not specified in the publication


enzyme_display_name: N-acetylglucosamine deacetylase

substrate_name: N-acetyl-D-glucosamine

enzyme: N-acetylglucosamine deacetylase

substrate: N-acetyl-D-glucosamine


organism: Cyclobacterium marinum

proof_lines: pH 8.0, 42°C, recombinant wild-type dimeric enzyme


proof_lines: pH 8.0, 42°C, recombinant K219E mutant enzyme


organism: Thermococcus kodakarensis


enzyme_display_name: alkylamidase

organism: Ovis aries aries

substrate_name: N-Butylcaproamide

enzyme: alkylamidase

substrate: N-Butylcaproamide


enzyme_display_name: phthalyl amidase

organism: Xanthobacter agilis

enzyme: phthalyl amidase


substrate_name: o-phthalyl carbacephem

substrate: o-phthalyl carbacephem


enzyme_display_name: cysteine-S-conjugate beta-lyase

organism: Escherichia coli

substrate_name: L-Cystathionine

enzyme: cysteine-S-conjugate beta-lyase

substrate: L-Cystathionine


substrate_name: L-cystathionine

proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W188F/W230F/W300F/W340F

substrate: L-cystathionine


proof_lines: pH 8.5, 25°C, recombinant mutant W188F/W230F/W276F/W300F/W340F


proof_lines: pH 8.5, 25°C, recombinant wild-type enzyme


proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W230F/W276F/W300F


proof_lines: pH 8.5, 25°C, recombinant mutant W131FF/W230FF/W276F


organism: Candidatus Pelagibacter communis

substrate_name: cystathionine

proof_lines: recombinant MBP-tagged enzyme, pH 8.0, 37°C

substrate: cystathionine


organism: Candidatus Pelagibacter ubique


proof_lines: pH 8.5, 25°C, recombinant chimeric mutant enzyme eCGS-sC-eCBL

proof_lines: pH 8.5, 25°C, recombinant chimeric mutant enzyme eCGS-sN-eCBL


proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W188F/W230F/W276F/W300F/W340F


proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W188F/W230F/W276F


organism: Spinacia oleracea


organism: Wolbachia pipientis

substrate_name: H2O; L-Cystathionine

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C

substrate: H2O; L-Cystathionine


organism: Lacticaseibacillus casei

substrate_name: L-cysteine

assay_conditions_summary: pH 5.5; 310.15 K; buffer sodium phosphate

proof_lines: Ctl2, in sodium phosphate buffer, at pH 6.8 and 37°C

proof_lines: Ctl2, in sodium phosphate buffer, at pH 5.5 and 37°C

substrate: L-cysteine


organism: Latilactobacillus sakei

substrate_name: L-Cysteine

substrate: L-Cysteine