enzyme_display_name: L-threo-3-deoxy-hexylosonate aldolase

organism: Trichoderma reesei

enzyme: L-threo-3-deoxy-hexylosonate aldolase


enzyme_display_name: aklavinone 12-hydroxylase

organism: Streptomyces peucetius

substrate_name: flavokermesic acid

source_db: brenda

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

protein_accession_database: UniProt

source_license: CC BY 4.0

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication

enzyme: aklavinone 12-hydroxylase

substrate: flavokermesic acid

source: brenda


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution

proof_lines: mutant P217K, pH not specified in the publication, temperature not specified in the publication


enzyme_display_name: ferruginol synthase

organism: Salvia miltiorrhiza

source_license: CC BY 4.0; CC BY-NC-ND 4.0

proof_lines:
pH 7.5, 28°C
Raw SKiD Env: Temp=28°C, pH=7.5

source_databases_merged: brenda skid

enzyme: ferruginol synthase


enzyme_display_name: anthranilate N-methyltransferase

organism: Ruta graveolens

substrate_name: S-adenosyl-L-methionine

enzyme: anthranilate N-methyltransferase

substrate: S-adenosyl-L-methionine


enzyme_display_name: dimethylglycine N-methyltransferase

organism: Parasynechococcus marenigrum WH 8102

enzyme: dimethylglycine N-methyltransferase


organism: Saccharomyces cerevisiae

proof_lines: 37°C, pH not specified in the publication, cloned enzyme

proof_lines: 37°C, pH not specified in the publication, native enzyme

proof_lines: 37°C, pH not specified in the publication, native and cloned enzyme


organism: Methanosarcina barkeri

substrate_name: methanol

proof_lines:
beta subunit MtaB
Raw SKiD Env: Temp=-----, pH=-----

substrate: methanol


enzyme_display_name: lipid-A-disaccharide synthase

organism: Escherichia coli

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

enzyme: lipid-A-disaccharide synthase


organism: Petrotoga mobilis

substrate_name: GDP-mannose

substrate: GDP-mannose


organism: Rhodopirellula baltica


organism: Homo sapiens

substrate_name: nicotinate

assay_conditions_summary: pH 5; 295.15 K; cofactor ADP

substrate: nicotinate


organism: Oryctolagus cuniculus

substrate_name: NGDP+

assay_conditions_summary: pH 7.4; cofactor ADP

proof_lines: pH 7.4, temperature not specified in the publication

substrate: NGDP+


enzyme_display_name: erythromycin 12 hydroxylase

organism: Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)

substrate_name: erythromycin D

source_db: uniprot

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

enzyme: erythromycin 12 hydroxylase

substrate: erythromycin D

source: uniprot


organism: Sinomonas cyclohexanicum

proof_lines: kcat is 55 sec(-1) with trans-4-hydroxyCHCA as substrate. kcat is 0.026 sec(-1) with cis-4-hydroxyCHCA as substrate. kcat is 650 sec(-1) with 4-oxoCHCA as substrate.


organism: Amycolatopsis mediterranei (strain S699)

substrate_name: 27-O-demethylrifamycin SV

substrate: 27-O-demethylrifamycin SV


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)


enzyme_display_name: phenylpropanoylacetyl-CoA synthase

organism: Curcuma longa

substrate_name: malonyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: phenylpropanoylacetyl-CoA synthase

substrate: malonyl-CoA


enzyme_display_name: crocetin glucosyltransferase

organism: Crocus sativus

substrate_name: crocetin

proof_lines: These parameters were determined on purified activity containing a mix of 2 enzymes, probably GLT2 and GLT3.

enzyme: crocetin glucosyltransferase

substrate: crocetin


enzyme_display_name: starch synthase (maltosyl-transferring)

organism: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

substrate_name: maltose 1-phosphate

proof_lines: The data are not very reliable because measures were done with a partially purified enzyme.

enzyme: starch synthase (maltosyl-transferring)

substrate: maltose 1-phosphate


enzyme_display_name: verruculogen prenyltransferase

organism: Aspergillus fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181)

substrate_name: dimethylallyl diphosphate

enzyme: verruculogen prenyltransferase

substrate: dimethylallyl diphosphate


enzyme_display_name: adenosylcobinamide kinase

organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

substrate_name: adenosylcobinamide

assay_conditions_summary: cofactor ATP

proof_lines: kcat is 15.1 min(-1) for adenosylcobinamide kinase activity. kcat is 5.6 min(-1) for guanylyltransferase activity.

enzyme: adenosylcobinamide kinase

substrate: adenosylcobinamide


organism: Mycolicibacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 / 3849)

substrate_name: MMP (at 37 degrees Celsius)

proof_lines: kcat is 0.011 sec(-1) (at 37 degrees Celsius). kcat is 0.021 sec(-1) (at 45 degrees Celsius).

substrate: MMP (at 37 degrees Celsius)


enzyme_display_name: funoran endo-beta-hydrolase

organism: Wenyingzhuangia aestuarii

substrate_name: funoran

proof_lines: kcat is 3.95 sec(-1) with funoran as substrate (PubMed:37479453). kcat is 176.74 sec(-1) with agarose as substrate (PubMed:32578425).

enzyme: funoran endo-beta-hydrolase

substrate: funoran


organism: Unknown

substrate_name: beta-Alanine

substrate: beta-Alanine


organism: Rattus norvegicus

substrate_name: pyruvate

substrate: pyruvate


enzyme_display_name: quercetin-3-sulfate 4'-sulfotransferase

enzyme: quercetin-3-sulfate 4'-sulfotransferase


enzyme_display_name: (deoxy)adenylate kinase

organism: Mus musculus

substrate_name: ATP

enzyme: (deoxy)adenylate kinase

substrate: ATP


enzyme_display_name: 2-phosphoglycerate kinase

organism: Methanothermus fervidus

assay_conditions_summary: pH 7.5; 348.15 K; cofactor ATP

enzyme: 2-phosphoglycerate kinase


organism: Aeropyrum pernix

substrate_name: CTP

proof_lines:
in presence of Mg2+, pH 7.5, 37°C
in absence of Mg2+, pH 7.5, 37°C

substrate: CTP


organism: Archaeoglobus fulgidus


organism: Candida albicans

substrate_name: CDP-diacylglycerol

substrate: CDP-diacylglycerol


organism: Homo sapiens

substrate_name: D-myo-Inositol 3,4-bisphosphate

substrate: D-myo-Inositol 3,4-bisphosphate


substrate_name: D-myo-Inositol 1,3,4-trisphosphate

substrate: D-myo-Inositol 1,3,4-trisphosphate


enzyme_display_name: kanosamine-6-phosphate phosphatase

organism: Bacillus cereus

substrate_name: kanosamine 6-phosphate

proof_lines: pH and temperature not specified in the publication

enzyme: kanosamine-6-phosphate phosphatase

substrate: kanosamine 6-phosphate


organism: Bacillus subtilis

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Corynebacterium striatum

assay_conditions_summary: pH 7

proof_lines: pH 7.0, temperature not specified in the publication


enzyme_display_name: cyanophycinase

organism: Synechocystis sp. PCC 6803

proof_lines: 30°C in 50 mM N-[2-hydroxy-1,1-bis(hydroxymethyl) ethyl]glycine, pH 8.0, maximum at pH 9.0

proof_lines: 30°C in 50 mM N-[2-hydroxy-1,1-bis(hydroxymethyl) ethyl]glycine, maximum at pH 8.0

enzyme: cyanophycinase


organism: Ectopseudomonas oleovorans


organism: Flaveria chlorifolia


organism: Escherichia coli

substrate_name: L-Ser

substrate: L-Ser


substrate_name: ATP; dAMP

substrate: ATP; dAMP


enzyme_display_name: acetoacetyl-CoA hydrolase

substrate_name: acetoacetyl-CoA

proof_lines:
liver cytosol, absence of glutathione
liver cytosol, presence of glutathione
brain mitochondria

enzyme: acetoacetyl-CoA hydrolase

substrate: acetoacetyl-CoA


organism: Comamonas testosteroni


enzyme_display_name: rifamycin-B oxidase

organism: Curvularia lunata

substrate_name: Rifamycin B

enzyme: rifamycin-B oxidase

substrate: Rifamycin B


organism: Humicola spp.

proof_lines:
free acetone-defatted cells
immobilized acetone-defatted cells


enzyme_display_name: benzylsuccinate synthase

organism: Thauera aromatica

substrate_name: Toluene

km_display: Not reported

proof_lines:
cell extract, 4°C
cell extract, 30°C

enzyme: benzylsuccinate synthase

substrate: Toluene


organism: Geobacter metallireducens

assay_conditions_summary: pH 7; 303.15 K; buffer 50 mM potassium phosphate; cofactor CoA

proof_lines: in 50 mM potassium phosphate buffer (pH 7.0), at 30°C


organism: Syntrophus aciditrophicus


organism: Drosophila melanogaster

substrate_name: L-Dopa

proof_lines: wild type enzyme, pH and temperature not specified in the publication

substrate: L-Dopa


proof_lines: mutant enzyme N192H, pH and temperature not specified in the publication


organism: Streptomyces griseus

substrate_name: glycerone phosphate

substrate: glycerone phosphate


organism: Cereibacter sphaeroides


organism: Sus scrofa

proof_lines: Y332F DDC mutant, reaction in 50 mM Hepes, pH 7.5, at 25°C causes the production of ammonia and 3,4-dihydroxyphenylacetaldehyde along with the consumption of molecular oxygen in a 1:2 molar ratio


enzyme_display_name: 1,8-cineole 2-endo-monooxygenase

organism: Citrobacter braakii

substrate_name: [reduced NADPH-hemoprotein reductase]

enzyme: 1,8-cineole 2-endo-monooxygenase

substrate: [reduced NADPH-hemoprotein reductase]


organism: Pseudomonas sp. YGJ3


organism: Oryza sativa Japonica Group

substrate_name: myo-inositol

proof_lines: recombinant enzyme from Escherichia coli, at pH 7.4 and 30°C

proof_lines: recombinant enzyme from Saccharomyces cerevisiae, at pH 7.4 and 30°C

substrate: myo-inositol


enzyme_display_name: beta-carotene 3-hydroxylase

organism: Agrobacterium aurantiacum

substrate_name: echinenone

enzyme: beta-carotene 3-hydroxylase

substrate: echinenone


enzyme_display_name: acyl-lipid omega-6 desaturase (cytochrome b5)

organism: Carthamus tinctorius


organism: Yarrowia lipolytica

enzyme: acyl-lipid omega-6 desaturase (cytochrome b5)


enzyme_display_name: hydroxylamine oxidase (cytochrome)

organism: Paracoccus denitrificans

substrate_name: hydroxylamine

proof_lines: pH 8, temperature not specified in the publication

enzyme: hydroxylamine oxidase (cytochrome)

substrate: hydroxylamine


organism: Pseudomonas sp.

substrate_name: horse heart ferricytochrome c

substrate: horse heart ferricytochrome c


enzyme_display_name: CoA-glutathione reductase

organism: Rattus norvegicus

substrate_name: CoA-glutathione

assay_conditions_summary: pH 5.75; cofactor CoA

proof_lines: at pH 5.75 and a fixed value of 110 nmol NADPH

enzyme: CoA-glutathione reductase

substrate: CoA-glutathione


organism: Saccharomyces cerevisiae

assay_conditions_summary: pH 5.5; cofactor NADPH

proof_lines: at pH 5.5 and a fixed value of 110 nmol NADPH


organism: Staphylococcus aureus

substrate_name: CoA disulfide

assay_conditions_summary: cofactor NADPH

substrate: CoA disulfide


enzyme_display_name: thiocyanate desulfurase

organism: Thioalkalivibrio paradoxus

substrate_name: ferricytochrome c 550

enzyme: thiocyanate desulfurase

substrate: ferricytochrome c 550


organism: Thioalkalivibrio sp.

substrate_name: Unknown

ki_unit: mM

substrate: Unknown


enzyme_display_name: starch synthase (maltosyl-transferring)

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: maltohexaose (in the presence of 5 mM M1P)

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

enzyme: starch synthase (maltosyl-transferring)


organism: Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

substrate: maltohexaose (in the presence of 5 mM M1P)


organism: Aplysia californica

substrate_name: cADPR (hydrolysis of cADPR)

substrate: cADPR (hydrolysis of cADPR)


organism: Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)

substrate_name: fumigaclavine A

substrate: fumigaclavine A


organism: Petroselinum crispum

substrate_name: umbelliferone in the reaction producing demethylsuberosin


substrate_name: D-beta-aminoisobutyrate

substrate: D-beta-aminoisobutyrate


organism: Methanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S)


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: CDP-diacylglycerol

substrate: CDP-diacylglycerol


organism: Flaveria chlorifolia

substrate_name: quercetin 3-sulfate

substrate: quercetin 3-sulfate


substrate_name: inositol 3,4-bisphosphate

substrate: inositol 3,4-bisphosphate


organism: Cellvibrio japonicus (strain Ueda107)

substrate_name: 2,4-dinitrophenyl-beta-galactobioside (at pH 7 and 37 degrees Celsius)


organism: Methanosarcina acetivorans

substrate_name: CoM-S-S-CoB

proof_lines: subunit HdrB2C2, at 21°C, pH not specified in the publication

proof_lines: subunit HdrB2, at 21°C, pH not specified in the publication

substrate: CoM-S-S-CoB


enzyme_display_name: tryptophan 2-C-methyltransferase

organism: Streptomyces laurentii

substrate_name: S-adenosyl-L-methionine

enzyme: tryptophan 2-C-methyltransferase

substrate: S-adenosyl-L-methionine


organism: Zea mays


organism: Cinchona succirubra

substrate_name: emodin

proof_lines:
isoform I
isoform III
isoform V

substrate: emodin


organism: Neomoorella thermoacetica

substrate_name: tetrahydrosarcinapterin

substrate: tetrahydrosarcinapterin


organism: Corynebacterium striatum


organism: Rattus norvegicus


organism: Pseudomonas anguilliseptica

substrate_name: cyanophycin granule polypeptide (CGP)

substrate: cyanophycin granule polypeptide (CGP)


organism: Comamonas testosteroni


organism: Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15)


organism: Syntrophus aciditrophicus (strain SB)


organism: Arabidopsis thaliana

substrate_name: L-phenylalanine

substrate: L-phenylalanine


organism: Petroselinum crispum

substrate_name: L-tyrosine

substrate: L-tyrosine


organism: Streptomyces griseus

substrate_name: L-aspartate-4-semialdehyde (at 30 degrees Celsius)


enzyme_display_name: N-acylsphingosine galactosyltransferase

organism: Gallus gallus

substrate_name: UDPgalactose

enzyme: N-acylsphingosine galactosyltransferase

substrate: UDPgalactose


proof_lines: Km value for ceramide is influenced by its dispersion state, in presence of 2 mM Triton S-100 biphasic kinetics are observed with increasing ceramide concentration, yielding two apparent Km values of 0.018 and 0.15 mM


enzyme_display_name: histidine transaminase

enzyme: histidine transaminase


organism: Streptomyces tendae

substrate_name: pyruvate

substrate: pyruvate


enzyme_display_name: guanosine phosphorylase

organism: Halorubrum lacusprofundi

substrate_name: phosphate

enzyme: guanosine phosphorylase

substrate: phosphate


organism: Oryctolagus cuniculus

substrate_name: deoxyguanosine

substrate: deoxyguanosine


enzyme_display_name: 2-amino-4-deoxychorismate synthase

organism: Burkholderia lata

substrate_name: chorismate

enzyme: 2-amino-4-deoxychorismate synthase

substrate: chorismate


enzyme_display_name: thiol sulfotransferase

organism: Cyanophora paradoxa

enzyme: thiol sulfotransferase


organism: Synechococcus sp.

assay_conditions_summary: pH 8


enzyme_display_name: 11-cis-retinyl-palmitate hydrolase

organism: Bos taurus

substrate_name: 11-cis-retinyl palmitate

enzyme: 11-cis-retinyl-palmitate hydrolase

substrate: 11-cis-retinyl palmitate


proof_lines:
enzyme from retinal pigment epithelium
enzyme from retina


organism: Homo sapiens


organism: Escherichia coli

substrate_name: acyl-carrier protein

substrate: acyl-carrier protein


organism: Pseudomonas aeruginosa

substrate_name: bis-4-nitrophenyl phosphate

substrate: bis-4-nitrophenyl phosphate


enzyme_display_name: CMP-N-acylneuraminate phosphodiesterase

substrate_name: CMP-N-acylneuraminate

enzyme: CMP-N-acylneuraminate phosphodiesterase

substrate: CMP-N-acylneuraminate


organism: Neisseria meningitidis


organism: Rattus norvegicus


enzyme_display_name: atrolysin B

organism: Crotalus atrox

substrate_name: oxidized insulin B-chain

proof_lines:
Gly23-Phe24 bond
His5-Leu6 bond
His10-Leu11 bond

enzyme: atrolysin B

substrate: oxidized insulin B-chain


enzyme_display_name: cerevisin

organism: Debaryomyces hansenii

enzyme: cerevisin


organism: Saccharomyces cerevisiae

substrate_name: benzoyl-Tyr ethyl ester

substrate: benzoyl-Tyr ethyl ester


enzyme_display_name: atrolysin E

substrate_name: dimethylcasein

proof_lines:
cobaltous toxin
Tyr14-Gln15 bond
Ala8-Ser9 bond

enzyme: atrolysin E

substrate: dimethylcasein


enzyme_display_name: trimerelysin I

organism: Protobothrops flavoviridis

enzyme: trimerelysin I


enzyme_display_name: N-acyl-D-aspartate deacylase

organism: Achromobacter xylosoxidans

substrate_name: N-Formyl-D-Asp

enzyme: N-acyl-D-aspartate deacylase

substrate: N-Formyl-D-Asp


enzyme_display_name: imidazolonepropionase

organism: Pseudomonas fluorescens

substrate_name: 4(5)-imidazolone-5(4)-propionic acid

enzyme: imidazolonepropionase

substrate: 4(5)-imidazolone-5(4)-propionic acid


organism: Salmonella enterica subsp. enterica serovar Typhimurium


enzyme_display_name: [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine

organism: Mycobacterium tuberculosis

substrate_name: O-phospho-L-serine

substrate: O-phospho-L-serine


enzyme: [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine


mutation_type: deletion

proof_lines: deletion mutant DELTA319-323, at pH 7.5 and 37°C


proof_lines: L-glutamine as ammonia source, pH 7.0, 25°C

proof_lines: ammonium sulfate as ammonia source, pH 7.0, 25°C

proof_lines: L-glutamine as ammonia source, in the presence of Mg2+, pH 7.0, 25°C


organism: Pseudomonas putida


enzyme_display_name: nucleoside phosphoacylhydrolase

substrate_name: guanosine 5'-p-nitrophenylphosphate

enzyme: nucleoside phosphoacylhydrolase

substrate: guanosine 5'-p-nitrophenylphosphate


enzyme_display_name: 6-methylsalicylate decarboxylase

organism: Penicillium griseofulvum

substrate_name: orsellinic acid

enzyme: 6-methylsalicylate decarboxylase

substrate: orsellinic acid


organism: Acinetobacter calcoaceticus

substrate_name: malonate

assay_conditions_summary: pH 6.8; 303.15 K; cofactor CoA

proof_lines: subunits beta/gamma in presence of acetyl-CoA

substrate: malonate


enzyme_display_name: 2-dehydro-3-deoxy-L-pentonate aldolase

organism: Pseudomonas sp.

enzyme: 2-dehydro-3-deoxy-L-pentonate aldolase


enzyme_display_name: prolycopene isomerase

organism: Solanum lycopersicum

substrate_name: Prolycopene

enzyme: prolycopene isomerase

substrate: Prolycopene


enzyme_display_name: lysine racemase

organism: Oenococcus oeni

substrate_name: D-Lysine

proof_lines: pH 8.0, 30°C, recombinant mutant T224I/W355Y

enzyme: lysine racemase

substrate: D-Lysine


enzyme_display_name: UniProt Q04HB7

substrate_name: L-lysine

source_db: skid

source_license: CC BY-NC-ND 4.0

enzyme: UniProt Q04HB7

substrate: L-lysine

source: skid


organism: Proteus mirabilis

substrate_name: L-arginine

proof_lines: pH 9.0, 50°C, recombinant His-tagged mutant T391Y

substrate: L-arginine


proof_lines: pH 9.0, 50°C, recombinant His-tagged mutant T391Y/S394Y


proof_lines: pH 8.0, 50°C, recombinant wild-type enzyme

proof_lines: pH 9.0, 50°C, recombinant His-tagged wild-type enzyme


enzyme_display_name: L-Lysine = D-Lysine

organism: Thermotoga maritima

substrate_name: L-Lysine

enzyme: L-Lysine = D-Lysine

substrate: L-Lysine


organism: uncultured bacterium

proof_lines: in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C


organism: Lentinus sajor-caju

substrate_name: phosphate

proof_lines:
pH 7
pH 6.3

substrate: phosphate


organism: Pleurotus sajor-caju

substrate_name: Phosphate; alpha,alpha-Trehalose

substrate: Phosphate; alpha,alpha-Trehalose


organism: Schizophyllum commune

substrate_name: alpha-D-glucose 1-phosphate

proof_lines:
30°C, pH 6.6, recombinant enzyme
30°C, pH 6.6, natural enzyme

substrate: alpha-D-glucose 1-phosphate


enzyme_display_name: fucose-1-phosphate guanylyltransferase

organism: Sus scrofa

substrate_name: Diphosphate; GDP-L-fucose

enzyme: fucose-1-phosphate guanylyltransferase

substrate: Diphosphate; GDP-L-fucose


organism: Vitis vinifera

substrate_name: GTP

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: GTP


enzyme_display_name: bile-salt sulfotransferase

organism: Homo sapiens

enzyme: bile-salt sulfotransferase


enzyme_display_name: proline racemase

organism: Clostridioides difficile

proof_lines: substrate: D-proline, Vmax= 0.000017 M/sec

proof_lines: substrate: L-proline, Vmax = 0.000027 M/sec

enzyme: proline racemase


substrate_name: D-proline

substrate: D-proline


organism: Ferroplasma acidarmanus

substrate_name: L-proline

substrate: L-proline


organism: Thermococcus litoralis


organism: Trypanosoma cruzi

substrate_name: L-Proline

substrate: L-Proline


proof_lines:
pH 6.0, 37°C
pH 6.0, 37°C, TcPRACB
pH 6.0, 37°C, TcPRACA


organism: Trypanosoma vivax

proof_lines: 40 mM substrate in 0.2 M sodium acetate, for 30 min at 37°C

proof_lines: 0.005 mM pyrrole-2-carboxylic acid, serial concentrations of 20-160 mM L-proline


proof_lines: pH 8.0, 30°C, recombinant wild-type enzyme


organism: Unknown

substrate_name: GDP-L-fucose

substrate: GDP-L-fucose


substrate_name: trehalose (at pH 7.0)

substrate: trehalose (at pH 7.0)


organism: Arabidopsis thaliana

substrate_name: L-fucose for the L-fucokinase activity

substrate: L-fucose for the L-fucokinase activity


substrate_name: DHEA

substrate: DHEA


organism: Rattus norvegicus

substrate_name: 3'-phosphoadenylyl sulfate

substrate: 3'-phosphoadenylyl sulfate


substrate_name: alpha,alpha-trehalose

substrate: alpha,alpha-trehalose


proof_lines: wild-type, Kcat/Km (phosphate): 21000/Msec


proof_lines: mutant R507A, Kcat/Km (phosphate): 0.0004/Msec


substrate_name: Unknown

substrate: Unknown


organism: Agaricus bisporus

substrate_name: alpha-D-glucose

substrate: alpha-D-glucose


organism: Flammulina velutipes


organism: Lactobacillus acidophilus

proof_lines: loop 3 variant TP3, in 40 mM phosphate-citrate pH 6.2 at 45°C

proof_lines: loop 3 variant TP2, in 40 mM phosphate-citrate pH 6.2 at 45°C


organism: Pleurotus ostreatus


substrate_name: fucose 1-phosphate

substrate: fucose 1-phosphate


organism: Bacteroides fragilis

substrate_name: beta-L-fucose 1-phosphate

substrate: beta-L-fucose 1-phosphate


substrate_name: mannose 1-phosphate

substrate: mannose 1-phosphate


organism: Mortierella alpina


organism: Acetoanaerobium sticklandii

substrate_name: L-Pro

proof_lines: pH and temperature not specified in the publication

substrate: L-Pro


proof_lines: recombinant His-tagged enzyme, pH 6.0, 37°C


enzyme_display_name: phosphomethylpyrimidine synthase

organism: Salmonella enterica

proof_lines: pH 8.0, 37°C, recombinant His-tagged enzyme

enzyme: phosphomethylpyrimidine synthase


substrate_name: CoA

substrate: CoA


enzyme_display_name: 7,8-dihydroneopterin epimerase

organism: Escherichia coli

substrate_name: 7,8-dihydroneopterin triphosphate

enzyme: 7,8-dihydroneopterin epimerase

substrate: 7,8-dihydroneopterin triphosphate


organism: Haemophilus influenzae


enzyme_display_name: flavanone 3-dioxygenase

organism: Albuca bracteata

enzyme: flavanone 3-dioxygenase


organism: Oryza sativa

substrate_name: (S)-eriodictyol

substrate: (S)-eriodictyol


enzyme_display_name: guanidinopropionase

organism: Candida parapsilosis

proof_lines: 3-guanidinopropionase, pH and temperature not specified in the publication

enzyme: guanidinopropionase


organism: Pseudomonas aeruginosa

proof_lines: pH 7.9, 30°C, wild-type unmethylated GpuA


source_db: oed

smiles: N=C(N)NCCCC(=O)[O-]

source: oed


smiles: N=C(N)NCCC(=O)[O-]


proof_lines: pH 7.9, 30°C, mutant Y157M unmethylated GpuA


organism: Petunia x hybrida


organism: Petroselinum crispum


organism: Arabidopsis thaliana


organism: Artemisia annua

substrate_name: naringenin

proof_lines: pH 8.5, 35°C, recombinant His-tagged enzyme

substrate: naringenin


organism: Glycine max

substrate_name: eriodictyol

substrate: eriodictyol


substrate_name: dihydroquercetin

substrate: dihydroquercetin


organism: Escherichia coli (strain K12)

substrate_name: 7,8-dihydroneopterin in aldolase reaction


organism: Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

substrate: 7,8-dihydroneopterin in aldolase reaction


organism: Methanothermobacter thermautotrophicus

assay_conditions_summary: pH 8; 338.15 K; cofactor CoA

proof_lines: pH 8.0, 65°C, substrate phenylpyruvate, benzyl viologen as electron acceptor


organism: Pyrococcus furiosus

substrate_name: phenylpyruvate

assay_conditions_summary: pH 8.4; 353.15 K; cofactor CoA

proof_lines:
pH 8.4, 80°C
pH 8.4, 80°C, substrate phenylpyruvate
pH 8.4, 80°C, substrate indolepyruvate

substrate: phenylpyruvate


organism: Thermococcus profundus

substrate_name: oxidized ferredoxin

assay_conditions_summary: pH 9; 353.15 K; buffer 50 mM TAPS; cofactor CoA

proof_lines:
in 50 mM TAPS buffer (pH 9.0), at 80°C
pH 9.0, 80°C

substrate: oxidized ferredoxin


enzyme_display_name: stizolobate synthase

organism: Stizolobium hassjoo

assay_conditions_summary: cofactor NADP+

enzyme: stizolobate synthase


enzyme_display_name: scopoletin 8-hydroxylase

substrate_name: scopoletin

enzyme: scopoletin 8-hydroxylase

substrate: scopoletin


enzyme_display_name: [protein]-arginine 3-hydroxylase

organism: Homo sapiens

substrate_name: 2-Oxoglutarate; O2; RPS6-peptide

enzyme: [protein]-arginine 3-hydroxylase

substrate: 2-Oxoglutarate; O2; RPS6-peptide


organism: Streptomyces glaucescens

substrate_name: NADPH

assay_conditions_summary: pH 9; 298.15 K; cofactor NADPH

substrate: NADPH


organism: Sorghum bicolor

substrate_name: H+; NADPH; O2; 4-Hydroxyphenylacetaldoxime


enzyme_display_name: syn-pimaradiene 3-monooxygenase

organism: Oryza sativa

enzyme: syn-pimaradiene 3-monooxygenase


substrate_name: tetracenomycin A2

substrate: tetracenomycin A2


organism: Streptomyces globisporus

assay_conditions_summary: pH 6; 298.15 K; cofactor FAD


enzyme_display_name: vinorine hydroxylase

organism: Rauvolfia serpentina

substrate_name: vinorine

enzyme: vinorine hydroxylase

substrate: vinorine


enzyme_display_name: (+)-piperitol/(+)-sesamin synthase

organism: Sesamum indicum

substrate_name: (+)-pinoresinol

enzyme: (+)-piperitol/(+)-sesamin synthase

substrate: (+)-pinoresinol


enzyme_display_name: (+)-lariciresinol reductase

organism: Camellia sinensis var. sinensis

substrate_name: (+)-lariciresinol

enzyme: (+)-lariciresinol reductase

substrate: (+)-lariciresinol


enzyme_display_name: sulfide dehydrogenase

assay_conditions_summary: pH 8; 353.15 K; cofactor NADPH

enzyme: sulfide dehydrogenase


organism: Escherichia coli

substrate_name: cytosine967 in 16S rRNA

substrate: cytosine967 in 16S rRNA


enzyme_display_name: benzoylsuccinyl-CoA thiolase

organism: Geobacter metallireducens

substrate_name: succinyl-CoA

assay_conditions_summary: cofactor CoA

proof_lines: calculated, kinetic model: ternary complex mechanism, pH and temperature not specified in the publication

enzyme: benzoylsuccinyl-CoA thiolase

substrate: succinyl-CoA


enzyme_display_name: RING-type E3 ubiquitin transferase

proof_lines: 37°C, pH not specified in the publication

enzyme: RING-type E3 ubiquitin transferase


enzyme_display_name: sulfoacetaldehyde acetyltransferase

organism: Castellaniella defragrans

enzyme: sulfoacetaldehyde acetyltransferase


organism: Streptomyces niveus

substrate_name: demethyldecarbamoylnovobiocin

substrate: demethyldecarbamoylnovobiocin


enzyme_display_name: piperidine N-piperoyltransferase

organism: Piper nigrum

substrate_name: piperidine

enzyme: piperidine N-piperoyltransferase

substrate: piperidine


organism: uncultured bacterium


organism: Rattus norvegicus


substrate_name: sulfoacetaldehyde

substrate: sulfoacetaldehyde


organism: Allium cepa

substrate_name: 1-kestose (3a) (for 6G-FFT activity)

substrate: 1-kestose (3a) (for 6G-FFT activity)


organism: Saccharomyces cerevisiae


substrate_name: 1F(1-beta-D-fructofuranosyl)2 sucrose

substrate: 1F(1-beta-D-fructofuranosyl)2 sucrose


enzyme_display_name: ginsenoside 20-O-glucosyltransferase

organism: Panax ginseng

enzyme: ginsenoside 20-O-glucosyltransferase


enzyme_display_name: hydroxyjasmonate sulfotransferase

organism: Arabidopsis thaliana

enzyme: hydroxyjasmonate sulfotransferase


enzyme_display_name: caffeate CoA-transferase

organism: Acetobacterium woodii

assay_conditions_summary: pH 7.5; 313.15 K; cofactor CoA

enzyme: caffeate CoA-transferase


substrate_name: beta-D-glucopyranosyl abscisate

substrate: beta-D-glucopyranosyl abscisate


enzyme_display_name: pseudaminic acid synthase

organism: Campylobacter jejuni

enzyme: pseudaminic acid synthase


enzyme_display_name: tRNA nucleotidyltransferase

proof_lines: with tRNA-C-C-A-C2 or tRNA-C-C-A-C3, pH 8.0, 37°C

enzyme: tRNA nucleotidyltransferase


proof_lines: calculated as adenylyl groups removed from substrate


enzyme_display_name: all-trans-retinyl ester 13-cis isomerohydrolase

organism: Danio rerio

substrate_name: all-trans retinyl ester

substrate: all-trans retinyl ester


enzyme_display_name: hydroxymethylglutaryl-CoA hydrolase

proof_lines:
microsomal
soluble enzyme

enzyme: hydroxymethylglutaryl-CoA hydrolase


enzyme_display_name: fructose-2,6-bisphosphate 6-phosphatase

substrate_name: D-fructose 2,6-bisphosphate

assay_conditions_summary: pH 6

enzyme: fructose-2,6-bisphosphate 6-phosphatase

substrate: D-fructose 2,6-bisphosphate


organism: Hordeum vulgare

substrate_name: p-nitrophenyl-beta-D-glucopyranoside

substrate: p-nitrophenyl-beta-D-glucopyranoside


enzyme_display_name: 2,3-bisphosphoglycerate 3-phosphatase

organism: Homo sapiens

proof_lines: phosphatase activity of deglycosylated human HsMIPP1 protein

enzyme: 2,3-bisphosphoglycerate 3-phosphatase


organism: Bos taurus

substrate_name: UDP-D-xylose; Glc-beta-Ser(53)-EGF-like domain of bovine factor IX(45-87)

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)


substrate_name: 11-cis retinol

enzyme: all-trans-retinyl ester 13-cis isomerohydrolase

substrate: 11-cis retinol


enzyme_display_name: galactan endo-1,6-beta-galactosidase

organism: Penicillium oxalicum

substrate_name: de-arabinosylated gum arabic

enzyme: galactan endo-1,6-beta-galactosidase

substrate: de-arabinosylated gum arabic


enzyme_display_name: ginsenoside 6-O-glucosyltransferase

enzyme: ginsenoside 6-O-glucosyltransferase


enzyme_display_name: lavandulyl diphosphate synthase

organism: Lavandula x intermedia

substrate_name: dimethylallyl diphosphate

enzyme: lavandulyl diphosphate synthase

substrate: dimethylallyl diphosphate


substrate_name: L-serine

substrate: L-serine


enzyme_display_name: funoran endo-alpha-hydrolase

organism: Aquimarina sp. AD1

substrate_name: funoran

enzyme: funoran endo-alpha-hydrolase

substrate: funoran


organism: Pseudomonas aeruginosa


organism: Artemisia spiciformis


organism: Escherichia coli (strain K12 / MC4100 / BW2952)

substrate_name: phosphate in the degradative reaction

substrate: phosphate in the degradative reaction


organism: Escherichia coli (strain K12)

substrate_name: adenylyl

assay_conditions_summary: cofactor ATP

substrate: adenylyl


substrate_name: gamma-glutamyl-gamma-aminobutyrate

substrate: gamma-glutamyl-gamma-aminobutyrate


substrate_name: Cys-Gly (at pH 6.9 and 25 degrees Celsius)

proof_lines: kcat is 3400 min(-1) for Cys-Gly hydrolysis activity (at pH 6.9 and 25 degrees Celsius). kcat is 11500 min(-1) for Leu-Gly hydrolysis activity (at pH 6.9 and 25 degrees Celsius). kcat is 8150 min(-1) for Met-Gly hydrolysis activity (at pH 6


enzyme_display_name: cyanophycinase

organism: Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

substrate_name: cyanophycin (at 30 degrees Celsius and pH 8.0)

enzyme: cyanophycinase


proof_lines: Values obtained using the partial C-terminal enzyme sequence of 105-377.


enzyme_display_name: guanidinopropionase

organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

proof_lines: kcat is 106 sec(-1) with 3-guanidinopropanoate as substrate. kcat is 57.6 sec(-1) for 4-guanidinobutanoate as substrate.

enzyme: guanidinopropionase


enzyme_display_name: aminodeoxychorismate lyase

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: aminodeoxychorismate

proof_lines: kcat is 1.9 sec(-1) with aminodeoxychorismate as substrate. kcat is 1.46 sec(-1) with D-alanine as substrate. kcat is 0.25 sec(-1) with D-glutamate as substrate.

enzyme: aminodeoxychorismate lyase

substrate: aminodeoxychorismate


enzyme_display_name: 3-hydroxybutyryl-CoA dehydratase

proof_lines: kcat is 110 sec(-1) with butenoyl-CoA as substrate.

enzyme: 3-hydroxybutyryl-CoA dehydratase


enzyme_display_name: 7,8-dihydroneopterin epimerase

substrate_name: 7,8-dihydroneopterin in the aldolase reaction

proof_lines: kcat is 0.0054 sec(-1) for the aldolase reaction with 7,8-dihydroneopterin as substrate. kcat is 0.006 sec(-1) for the aldolase reaction with 7,8-dihydromonapterin as substrate. kcat is 0.0015 sec(-1) for the epimerization of 7,8-dihydroneo

enzyme: 7,8-dihydroneopterin epimerase


enzyme_display_name: glucan 1,3-alpha-glucosidase

organism: Amorphotheca resinae

substrate_name: pseudonigeran

enzyme: glucan 1,3-alpha-glucosidase

substrate: pseudonigeran


organism: Trichoderma sp.


organism: Trichoderma asperellum

substrate_name: H2O; Mutan

substrate: H2O; Mutan


enzyme_display_name: chenodeoxycholoyltaurine hydrolase

organism: Bifidobacterium longum

substrate_name: glycocholate (at 37 degrees Celsius and pH 5.5)

proof_lines: kcat is 85 sec(-1) with glycocholate as substrate. kcat is 76 sec(-1) with taurocholate as substrate (at 40 degrees Celsius and pH 6.5).

enzyme: chenodeoxycholoyltaurine hydrolase


enzyme_display_name: 4-hydroxyphenylacetaldehyde synthase

organism: Rhodiola rosea

substrate_name: L-tyrosine

proof_lines: kcat is 4.91 sec(-1) with L-tyrosine as substrate.

enzyme: 4-hydroxyphenylacetaldehyde synthase

substrate: L-tyrosine


substrate_name: CAPA

proof_lines: kcat is 2.36 sec(-1) with CAPA as substrate.

substrate: CAPA


organism: Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

proof_lines: kcat is 0.6 sec(-1) for 3-hydroxy-2-methylpyridine-4,5-dicarboxylate as substrate.


organism: Homo sapiens

substrate_name: Gppp-BODIPY

substrate: Gppp-BODIPY


organism: Petroselinum crispum


enzyme_display_name: siroheme decarboxylase

organism: Desulfovibrio desulfuricans (strain ATCC 27774 / DSM 6949 / MB)

substrate_name: siroheme

enzyme: siroheme decarboxylase

substrate: siroheme


organism: Synechocystis sp. PCC 6803

proof_lines: at pH 7.4, temperature not specified in the publication


organism: Desulfovibrio desulfuricans

proof_lines: pH and temperature not specified in the publication


organism: Mesorhizobium loti

proof_lines: in 100 mM Tris-HCl at pH 8.0 containing 100 mM NaCl and 2 mM dithiothreitol


organism: Pseudomonas sp.


organism: Nocardioides sp.


enzyme_display_name: sulfofructosephosphate aldolase

organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: 6-deoxy-6-sulfo-D-fructose 1-phosphate

enzyme: sulfofructosephosphate aldolase

substrate: 6-deoxy-6-sulfo-D-fructose 1-phosphate


organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

proof_lines: kcat is 47.7 sec(-1) with 6-deoxy-6-sulfo-D-fructose 1-phosphate as substrate.


enzyme_display_name: 2-halobenzoate 1,2-dioxygenase

organism: Burkholderia cepacia

assay_conditions_summary: cofactor NADH

proof_lines:
component A
component B

enzyme: 2-halobenzoate 1,2-dioxygenase


proof_lines: cells grown on 2-chlorobenzoate or 2,5-dichlorobenzoate


enzyme_display_name: 1-hydroxy-2-naphthoate hydroxylase

organism: Alcaligenes sp.

substrate_name: NADH

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADPH

proof_lines:
pH 7.5, 30°C
pH 7.5, 30°C, with NADPH
pH 7.5, 30°C, with NADH

enzyme: 1-hydroxy-2-naphthoate hydroxylase

substrate: NADH


enzyme_display_name: 4-hydroxyphenylacetate 1-monooxygenase

organism: Delftia acidovorans

enzyme: 4-hydroxyphenylacetate 1-monooxygenase


enzyme_display_name: propanediol-phosphate dehydrogenase

organism: Oryctolagus cuniculus

enzyme: propanediol-phosphate dehydrogenase


organism: uncultured bacterium


enzyme_display_name: peptide-tryptophan 2,3-dioxygenase

organism: Triticum aestivum

substrate_name: N-acetyltryptophan

enzyme: peptide-tryptophan 2,3-dioxygenase

substrate: N-acetyltryptophan


organism: Zea mays

assay_conditions_summary: pH 8.5; 308.15 K; cofactor NADPH

proof_lines: recombinant enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C

proof_lines: native enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C


enzyme_display_name: 2-nitrophenol 2-monooxygenase

organism: Pseudomonas putida

assay_conditions_summary: cofactor NADPH

enzyme: 2-nitrophenol 2-monooxygenase


enzyme_display_name: marmesin synthase

organism: Ficus carica

substrate_name: demethylsuberosin

enzyme: marmesin synthase

substrate: demethylsuberosin


enzyme_display_name: indolin-2-one monooxygenase

enzyme: indolin-2-one monooxygenase


organism: Ammi majus


enzyme_display_name: adenosylcobinamide kinase

assay_conditions_summary: pH 8.8; 310.15 K; cofactor ATP

enzyme: adenosylcobinamide kinase


enzyme_display_name: ginsenoside 20-O-glucosyltransferase

organism: Panax ginseng

substrate_name: protopanaxadiol

proof_lines: kcat is 9.4 sec(-1) with protopanaxadiol as substrate (PubMed:24603359). kcat is 12.7 sec(-1) with dammarenediol II as substrate (PubMed:24603359).

enzyme: ginsenoside 20-O-glucosyltransferase

substrate: protopanaxadiol


enzyme_display_name: pseudaminic acid synthase

organism: Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)

substrate_name: phosphoenolpyruvate

verification_status: unverified

enzyme: pseudaminic acid synthase

substrate: phosphoenolpyruvate

status: unverified


enzyme_display_name: fucose-1-phosphate guanylyltransferase

organism: Bacteroides fragilis

substrate_name: L-fucose

proof_lines: kcat is 0.39 sec(-1) for the fucokinase activity. kcat is 10.07 sec(-1) for the fucose-1-phosphate guanylyltransferase activity.

enzyme: fucose-1-phosphate guanylyltransferase

substrate: L-fucose


organism: Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)

substrate_name: DGGGP

substrate: DGGGP


organism: Escherichia coli (strain K12)

substrate_name: CDP-1,2-diacyl-sn-glycerol (at pH 7.5 and 25 degrees Celsius)

proof_lines: kcat is 69.7 sec(-1) for the CDP-1,2-diacyl-sn-glycerol and 59.8 sec(-1) for L-serine (at pH 7.5 and 25 degrees Celsius).


enzyme_display_name: caffeate CoA-transferase

organism: Acetobacterium woodii

substrate_name: hydrocaffeyl-CoA

proof_lines: kcat is 120 sec(-1) for transferase activity with caffeate as substrate.

enzyme: caffeate CoA-transferase

substrate: hydrocaffeyl-CoA


enzyme_display_name: all-trans-retinyl ester 13-cis isomerohydrolase

organism: Danio rerio

substrate_name: all-trans-retinyl ester

proof_lines: kcat is 0.00044 sec(-1) with all-trans-retinyl ester as substrate.

substrate: all-trans-retinyl ester


enzyme_display_name: acetoacetyl-CoA hydrolase

organism: Mus musculus

substrate_name: acetyl-CoA

proof_lines: In summary, KM for medium- to long-chain acyl CoAs is in the order 1.4-6.7 uM, and with short-chain acyl CoAs range from 8 to 30 uM. KM for bile acid-CoA esters is in the range 6-15 uM.

enzyme: acetoacetyl-CoA hydrolase

substrate: acetyl-CoA


enzyme_display_name: hydroxymethylglutaryl-CoA hydrolase

enzyme: hydroxymethylglutaryl-CoA hydrolase


enzyme_display_name: kanosamine-6-phosphate phosphatase

organism: Bacillus subtilis (strain 168)

proof_lines: kcat is 32 sec(-1) for dephosphorylation with kanosamine 6-phosphate (at pH 7.5 and 25 degrees Celsius).

enzyme: kanosamine-6-phosphate phosphatase


enzyme_display_name: funoran endo-alpha-hydrolase

organism: Aquimarina sp. (strain AD1)

substrate_name: funoran

proof_lines: kcat is 43.40 sec(-1) with funoran as substrate. kcat is 101.39 sec(-1) with agarose as substrate.

enzyme: funoran endo-alpha-hydrolase

substrate: funoran


organism: Bacillus subtilis

substrate_name: ginsenoside Rc

substrate: ginsenoside Rc


substrate_name: 4-nitrophenyl alpha-L-arabinofuranoside

substrate: 4-nitrophenyl alpha-L-arabinofuranoside


organism: Bifidobacterium longum


assay_conditions_summary: pH 5.5; 310.15 K; buffer 50 mM sodium acetate

proof_lines: in 50 mM sodium acetate buffer (pH 5.5), at 37°C


organism: Bifidobacterium longum subsp. Longum

substrate_name: larch type II arabinogalactan

substrate: larch type II arabinogalactan


organism: Chaetomium sp.


organism: Corynebacterium alkanolyticum


organism: Evansstolkia leycettana


organism: Geobacillus stearothermophilus


organism: Mycosarcoma maydis


organism: Acetivibrio thermocellus

proof_lines: pH 5.7, 50°C, recombinant full-length enzyme

proof_lines: pH 5.7, 50°C, recombinant catalytic module CtGH43


organism: Alicyclobacillus sp.


organism: Anoxybacillus kestanbolensis

substrate_name: p-nitrophenyl-alpha-L-arabinofuranoside

substrate: p-nitrophenyl-alpha-L-arabinofuranoside


organism: Aspergillus nidulans

substrate_name: rye arabinoxylan

substrate: rye arabinoxylan


organism: Aspergillus niger

assay_conditions_summary: pH 5; 323.15 K; buffer 50 mM acetate

proof_lines: in 50 mM acetate buffer, pH 5.0, at 50°C


organism: Aureobasidium pullulans


organism: Bacillus pumilus

substrate_name: p-nitrophenyl alpha-L-arabinofuranoside

substrate: p-nitrophenyl alpha-L-arabinofuranoside


substrate_name: linear alpha-1,5-L-arabinan

substrate: linear alpha-1,5-L-arabinan


enzyme_display_name: catalase peroxidase

organism: Mycobacterium tuberculosis

substrate_name: o-Dianisidine

proof_lines:
wild type enzyme, at pH 4.5 and 30°C
wild type enzyme, at pH 7.0 and 30°C

enzyme: catalase peroxidase

substrate: o-Dianisidine


organism: Neurospora crassa

proof_lines: pH 5.0, 25°C, recombinant intein-tagged enzyme

proof_lines: pH 5.5, 25°C, recombinant intein-tagged enzyme

proof_lines: pH 5.0, 25°C, with o-dianisidine, recombinant intein-tagged enzyme


organism: Pyricularia grisea


proof_lines: mutant C26A/C74A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant C55A/C74A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant C55A, pH not specified in the publication, temperature not specified in the publication


proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant C74A, pH not specified in the publication, temperature not specified in the publication


organism: Synechococcus elongatus

substrate_name: isoniazid

substrate: isoniazid


organism: Synechocystis sp.

assay_conditions_summary: pH 7; 298.15 K; buffer 25 mM HEPES-NaOH

proof_lines: wild type enzyme, in 25 mM HEPES-NaOH buffer (pH 7.0) at 25°C


organism: Talaromyces funiculosus

substrate_name: wheat arabinoxylan

proof_lines:
pH 4.0, 40°C, isozyme PfABF62a
pH 4.0, 40°C, isozyme PfABF62b
pH 4.0, 40°C, isozyme PfABF62c

substrate: wheat arabinoxylan


proof_lines: pH 4.0, 40°C, isozyme mutant pfabf62cDELTACBM


organism: Aspergillus terreus

proof_lines:
isoenzyme ABF1
isoenzyme ABF2
isoenzyme ABF3


organism: Fusarium oxysporum

proof_lines:
pH 6.0, 45°C, isozyme ABF2
pH 6.0, 45°C, isozyme ABF1


organism: Streptomyces diastaticus

substrate_name: phenyl-alpha-L-arabinofuranoside

proof_lines:
isoenzyme C2
isoenzyme C1

substrate: phenyl-alpha-L-arabinofuranoside


organism: Trichoderma reesei

proof_lines:
isoenzyme AF53
isoenzyme AF35


organism: Unknown

substrate_name: arabinobiose

substrate: arabinobiose


organism: Paenibacillus sp.

proof_lines: recombinant His6-tagged enzyme, pH 6.0, 60°C


organism: Penicillium parvum


organism: Pleurotus ostreatus


organism: Podospora anserina


organism: Pseudothermotoga thermarum


organism: Streptomyces coelicolor

substrate_name: wheat L-arabinoxylan

proof_lines: ph 5.5, 37°C, recombinant wild-type enzyme

proof_lines: pH 5.5, 37°C, recombinant wild-type enzyme

substrate: wheat L-arabinoxylan


substrate_name: L-arabinan

substrate: L-arabinan


organism: Talaromyces purpureogenus


assay_conditions_summary: pH 4; 303.15 K; buffer 50 mM sodium citrate

proof_lines: in 50 mM sodium citrate buffer (pH 4.0), at 30°C


organism: Thermobacillus xylanilyticus


proof_lines:
wild-type, pH 7.0, 45°C
pH 5.8, 60°C, wild-type enzyme


substrate_name: p-nitrophenyl-alpha-L-arabinofuranose

mutation_signature: Tx-Abf-M94_I103delinsGG mutant

mutation_type: delins

proof_lines:
Tx-Abf-M94_I103delinsGG mutant, 60°C
Tx-Abf-M94_I103delinsGG mutant

substrate: p-nitrophenyl-alpha-L-arabinofuranose


proof_lines:
Tx-Abf-C180A mutant, 60°C
Tx-Abf-C180A mutant


proof_lines:
Tx-Abf-W99A mutant, 60°C
Tx-Abf-W99A mutant


organism: Thermothielavioides terrestris


organism: uncultured rumen bacterium

assay_conditions_summary: pH 7; 313.15 K; buffer pH 7

proof_lines: in 50 mM phosphate/citrate buffer pH 7.0, at 40°C


organism: Weissella sp. 142


enzyme_display_name: flavivirin

organism: dengue virus type 1

enzyme: flavivirin


substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication

substrate: Unknown


organism: dengue virus type 2

proof_lines: pH 8.5, 37°C, recombinant linked form mutant S75C


proof_lines: pH 8.5, 37°C, recombinant unlinked form mutant K117A

proof_lines: pH 8.5, 37°C, recombinant linked form mutant K117A


proof_lines: pH 8.5, 37°C, recombinant linked form wild-type enzyme

proof_lines: pH 8.5, 37°C, recombinant unlinked form wild-type enzyme


proof_lines: pH 8.5, 37°C, recombinant linked form mutant K117C


proof_lines: pH 8.5, 37°C, recombinant unlinked form mutant A125C/V162C

proof_lines: pH 8.5, 37°C, recombinant linked form mutant A125C/V162C


proof_lines: pH 8.5, 37°C, recombinant unlinked form mutant I73C/P106C

proof_lines: pH 8.5, 37°C, recombinant linked form mutant I73C/P106C


proof_lines: pH 8.5, 37°C, recombinant linked form mutant K117R


proof_lines: pH 8.5, 37°C, recombinant unlinked form mutant S75C/K117C

proof_lines: pH 8.5, 37°C, recombinant linked form mutant S75C/K117C


proof_lines: pH 8.5, temperature not specified in the publication


organism: dengue virus type 3


organism: dengue virus type 4


organism: Langat virus


organism: Selenomonas ruminantium


organism: Mycobacterium tuberculosis


proof_lines: mutant enzyme E291K/E292A, at pH 5.0 and 23°C


organism: Escherichia coli

proof_lines: peroxidase activity, mutant enzyme H106C, at pH 6.0 and 25°C

proof_lines: catalase activity, mutant enzyme H106C, at pH 7.0 and 37°C


proof_lines: peroxidase activity, mutant enzyme H106Y, at pH 6.0 and 25°C

proof_lines: catalase activity, mutant enzyme H106Y, at pH 7.0 and 37°C


proof_lines: catalase activity, mutant enzyme H267Y, at pH 7.0 and 37°C


proof_lines: catalase activity, mutant enzyme R102K, at pH 7.0 and 37°C

proof_lines: peroxidase activity, mutant enzyme R102K, at pH 6.0 and 25°C


proof_lines: peroxidase activity, mutant enzyme R102L, at pH 6.0 and 25°C

proof_lines: catalase activity, mutant enzyme R102L, at pH 7.0 and 37°C


proof_lines: catalase activity, mutant enzyme R102C, at pH 7.0 and 37°C

proof_lines: peroxidase activity, mutant enzyme R102C, at pH 6.0 and 25°C


proof_lines: catalase activity, mutant enzyme W105L, at pH 7.0 and 37°C

proof_lines: peroxidase activity, mutant enzyme W105L, at pH 6.0 and 25°C


proof_lines: catalase activity, mutant enzyme W105F, at pH 7.0 and 37°C

proof_lines: peroxidase activity, mutant enzyme W105F, at pH 6.0 and 25°C


organism: Trichoderma koningii


proof_lines:
pH 6.5, wild-type enzyme
pH 4.1, 25°C, wild-type enzyme


proof_lines:
pH 6.5, mutant enzyme D299G
pH 4.1, 25°C, mutant enzyme D299G


proof_lines: recombinant mutants L634F and D735A, 23°C


organism: Alkhumra hemorrhagic fever virus

proof_lines: chimeric proteases with the NS2B cofactor from Langat virus replacing the Alkhurma virus sequence


organism: Synechocystis sp.


assay_conditions_summary: pH 7; 303.15 K; buffer 100 mM phosphate

proof_lines: catalase activity, mutant enzyme D152S, in 100 mM phosphate buffer, pH 7.0, at 23°C

proof_lines: mutant enzyme D152S, catalase activity, pH and temperature not specified in the publication


proof_lines: mutant R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C


proof_lines: mutant Y697A, N- and C-terminal domain separately expressed, pH 7.0, 23°C


proof_lines: mutant E695A, N- and C-terminal domain separately expressed, pH 7.0, 23°C


proof_lines: mutant L690A/R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C


assay_conditions_summary: pH 7; 303.15 K; buffer several

proof_lines: catalase activity, wild type enzyme, in 100 mM phosphate buffer, pH 7.0, at 23°C

proof_lines: catalase activity, wild type enzyme, in 50 mM phosphate buffer, pH 7.0, and 30°C


organism: Streptomyces avermitilis

proof_lines: wild type enzyme, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C


proof_lines: mutant enzyme N159L, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C


proof_lines: mutant enzyme L289A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C


proof_lines: mutant enzyme N159A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C


proof_lines: mutant enzyme Y192A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C


proof_lines: mutant enzyme D20N, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C


organism: Synechocystis sp. PCC 6803


proof_lines: mutant DELTA200-214, catalase activity, pH 7.0, 23°C


organism: Aspergillus luchuensis


assay_conditions_summary: pH 7; 296.15 K; buffer 100 mM phosphate

proof_lines: catalase activity, mutant enzyme R439A, in 100 mM phosphate buffer, pH 7.0, at 23°C


proof_lines: catalase activity, mutant enzyme Y249F, in 100 mM phosphate buffer, pH 7.0, at 23°C


proof_lines: catalase activity, mutant enzyme H123E, in 100 mM phosphate buffer, pH 7.0, at 23°C


assay_conditions_summary: pH 6; 298.15 K; buffer 10 mM phosphate

proof_lines: peroxidatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C

proof_lines: catalatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C


proof_lines: peroxidatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C

proof_lines: catalatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C


proof_lines: catalatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C

proof_lines: peroxidatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C


proof_lines: catalatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C

proof_lines: peroxidatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C


organism: Yellow fever virus


assay_conditions_summary: pH 5; 296.15 K; buffer pH 5

proof_lines: wild type enzyme, peroxidase activity, in 50 mM acetate buffer, pH 5.0, 23°C

proof_lines: peroxidase activity, wild type enzyme, in 50 mM acetate buffer pH 5.0, at 23°C


assay_conditions_summary: pH 5; 296.15 K; buffer 50 mM acetate

proof_lines: mutant enzyme Y111A, peroxidase activity, in 50 mM acetate buffer, pH 5.0, 23°C

proof_lines: mutant enzyme Y111A, catalase activity, in 100 mM phosphate buffer, pH 7.0, 23°C


assay_conditions_summary: pH 7.5; 310.15 K; buffer 50 mM sodium phosphate

proof_lines: mutant enzyme S315T, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C

proof_lines: mutant enzyme S315T, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C

proof_lines: mutant enzyme S315T, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C


proof_lines: wild type enzyme, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C

proof_lines: wild-type, N- and C-terminal domain separately expressed, pH 7.0, 23°C

proof_lines: catalase activity, wild type enzyme, in 100 mM phosphate buffer, pH 7.0, at 23°C


assay_conditions_summary: pH 7; 303.15 K; buffer phosphate

proof_lines: catalase activity, mutant enzyme N153A, in 50 mM phosphate buffer, pH 7.0, and 30°C


proof_lines: catalase activity, mutant enzyme N153D, in 50 mM phosphate buffer, pH 7.0, and 30°C


organism: Geobacillus stearothermophilus

substrate_name: 2,5-dinitrophenyl beta-D-xylopyranoside

substrate: 2,5-dinitrophenyl beta-D-xylopyranoside


organism: Cellvibrio japonicus


organism: West Nile virus

assay_conditions_summary: pH 8; 295.15 K; buffer 20 mM Tris/HCl

proof_lines: mutant enzyme K48A, in 20 mM Tris/HCl buffer, pH 8.0, at 22°C


proof_lines: wild type enzyme, in 20 mM Tris/HCl buffer, pH 8.0, at 22°C


organism: Archaeoglobus fulgidus

proof_lines: catalase reaction, at pH 5.5-6.0 and 37°C


organism: Bacillus pumilus


organism: Bradyrhizobium japonicum

proof_lines: catalase activity, pH and temperature not specified in the publication


organism: Burkholderia pseudomallei


proof_lines: peroxidase activity, mutant enzyme KatGDELTAFG, in 50 mM acetate buffer pH 5.0, at 23°C

proof_lines: catalase activity, mutant enzyme KatGDELTAFG, 100 mM phosphate buffer pH 7.0, at 23°C


substrate_name: pyrogallol

assay_conditions_summary: pH 4; 296.15 K; buffer 50 mM acetate

proof_lines:
pH 4.0, 23°C
peroxidase reaction, at pH 4.3 and 25°C
pH 6.0, 23°C

substrate: pyrogallol


organism: Haloarcula marismortui

proof_lines:
peroxidatic activity, pH 6.0, 25°C
catalatic activity, pH 7.5, 25°C


organism: Halobacterium salinarum

proof_lines: catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C

proof_lines: apparent value, peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C


organism: Klebsiella pneumoniae

proof_lines:
peroxidase activity, pH 5.3, 30°C
catalase activity, pH 6.3, 30°C


organism: Mycobacterium sp.

assay_conditions_summary: pH 7; 303.15 K; buffer 50 mM potassium phosphate

proof_lines: peroxidase activity, in 50 mM potassium phosphate buffer (pH 7.0), at 30°C

proof_lines: catalase activity, in 50 mM potassium phosphate buffer (pH 7.0), at 30°C


organism: Mycobacterium tuberculosis

proof_lines: peroxidatic activity, using tert-butyl hydroperoxide as cosubstrate, in 10 mM K2HPO4/KH2PO4 (pH 7.5), at 25°C


enzyme_display_name: heme oxygenase (biliverdin-producing, ferredoxin)

organism: Arabidopsis thaliana

substrate_name: hemin

proof_lines:
at pH 7.2 and 25°C
isoform HY1, at pH 7.2 and 25°C
isoform HO4, at pH 7.2 and 25°C

substrate: hemin


organism: Rhodobacter capsulatus


organism: Synechococcus elongatus PCC 7942 = FACHB-805

assay_conditions_summary: pH 7; 298.15 K; buffer 67 mM phosphate

proof_lines: catalase activity, in 67 mM phosphate buffer (pH 7.0), at 25°C


organism: Synechocystis sp.

assay_conditions_summary: pH 4.3; 298.15 K; buffer 67 mM phosphate


proof_lines: mutant enzyme E243Q, catalase activity, pH and temperature not specified in the publication


enzyme_display_name: acyl-lipid (8-3)-desaturase

organism: Homo sapiens

substrate_name: dihomo-gamma-linolenic acid

enzyme: acyl-lipid (8-3)-desaturase

substrate: dihomo-gamma-linolenic acid


organism: Rattus norvegicus

proof_lines: pH and temperature not specified in the publication


proof_lines: mutant enzyme R463L, catalase activity, in 50 mM Na2HPO4, pH 7.0, 25°C


organism: Mycolicibacterium smegmatis

substrate_name: NADPH

assay_conditions_summary: pH 7; 298.15 K; buffer 50 mM phosphate; cofactor NADPH

proof_lines: peroxidatic activity, in 50 mM phosphate buffer, pH 7.0, at 25°C

proof_lines: catalatic activity, in 50 mM phosphate buffer, pH 7.0, at 25°C

proof_lines: peroxidatic activity, with hydrogen peroxide as cosubstrate, in 50 mM phosphate buffer, pH 7.0, at 25°C

substrate: NADPH


organism: Oceanobacillus oncorhynchi subsp. incaldanensis

assay_conditions_summary: pH 9; 303.15 K; buffer 20 mM Tris-HCl

proof_lines: catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C


organism: Penicillium simplicissimum


organism: Aspergillus fumigatus

substrate_name: 4-nitrophenyl alpha-L-arabinofuranoside

proof_lines:
isoform AbfII, at pH 5.0 and 42°C
isoform AbfI, at pH 5.0 and 37°C

substrate: 4-nitrophenyl alpha-L-arabinofuranoside


organism: Aspergillus nidulans


organism: Aspergillus niger

substrate_name: beet arabinan

proof_lines:
strain B
alpha-L-arabinofuranosidase B
alpha-L-arabinofuranosidase A

substrate: beet arabinan


organism: Bacillus pumilus


organism: Bifidobacterium breve


organism: Butyrivibrio fibrisolvens


organism: Caldicellulosiruptor saccharolyticus

assay_conditions_summary: pH 5.5; 353.15 K; buffer 50 mM citric acid

proof_lines: in 50 mM citric acid buffer (pH 5.5), at 80°C


organism: Caldicellulosiruptor sp. F32


organism: Acetivibrio clariflavus


organism: Acetivibrio thermocellus


organism: Agroathelia rolfsii

substrate_name: phenyl alpha-L-arabinofuranoside

substrate: phenyl alpha-L-arabinofuranoside


organism: Arabidopsis thaliana

substrate_name: p-nitrophenyl-alpha-L-arabinofuranoside

substrate: p-nitrophenyl-alpha-L-arabinofuranoside


organism: Cellulosimicrobium aquatile

substrate_name: ginsenoside Rc

substrate: ginsenoside Rc


organism: Clostridium acetobutylicum


organism: Dichomitus squalens


organism: Fusarium graminearum


organism: Linum usitatissimum

proof_lines: in 0.1 M sodium acetate, pH 6 at 40°C for 30 min


organism: Monilinia fructigena

substrate_name: alpha-L-arabinofuranosyl 4-phenylphenol

substrate: alpha-L-arabinofuranosyl 4-phenylphenol


organism: Paenibacillus sp.


organism: Penicillium chrysogenum

proof_lines:
enzyme AFQ1, pH 5.0, 37°C
enzyme AFS1, pH 5.0, 37°C


organism: Saccharococcus caldoxylosilyticus


organism: Spinacia oleracea

proof_lines:
alpha-L-arafase II
alpha-L-arafase I


organism: Streptomyces purpurascens


organism: Streptomyces sp.


organism: Penicillium oxalicum


organism: Penicillium subrubescens

proof_lines:
isoform AbfF, pH 5, 30°C
isoform AbfA, pH 5, 30°C
isoform AbfC, pH 5, 30°C


organism: Rhodotorula flava


organism: Ruminococcus albus


organism: Streptomyces sp. PC22

assay_conditions_summary: pH 6


organism: Talaromyces amestolkiae

proof_lines:
isoform Ara2, pH 5, 50°C
isoform Ara1, pH 5, 50°C


organism: Talaromyces purpureogenus

substrate_name: 4-nitrophenyl beta-D-galactofuranoside

substrate: 4-nitrophenyl beta-D-galactofuranoside


organism: Thermobacillus xylanilyticus

substrate_name: p-nitrophenyl-alpha-L-arabinose

substrate: p-nitrophenyl-alpha-L-arabinose


organism: Thermobifida fusca

assay_conditions_summary: pH 9


organism: Thermomyces dupontii

assay_conditions_summary: pH 7; 323.15 K; buffer 50 mM potassium phosphate

proof_lines: in 50 mM potassium phosphate buffer (pH 7.0), at 50°C


organism: Trametes hirsuta

substrate_name: arabinoxylan

substrate: arabinoxylan


organism: Trichoderma sp.


organism: Geobacillus stearothermophilus


organism: Glycine max


organism: Hordeum vulgare


organism: Levilactobacillus brevis


organism: Bussuquara virus


organism: Dengue virus

proof_lines: pH 8.5, 37°C, enzyme from Dengue virus serotype 1

proof_lines: pH 8.5, 37°C, enzyme from Dengue virus serotype 3

proof_lines: pH 8.5, 37°C, enzyme from Dengue virus serotype 4


organism: dengue virus type 2

substrate_name: Gly-Arg-Arg-4-methylcoumarin 7-amide

proof_lines: pH 9.0, 37°C, wild-type NS3 protease with wild-tyüe NS2B cofactor

substrate: Gly-Arg-Arg-4-methylcoumarin 7-amide


proof_lines: predicted inhibition constant, pH and temperature not specified in the publication


organism: Zika virus

proof_lines: pH and temperature not specified in the publication


organism: Murray Valley encephalitis virus


organism: St. Louis encephalitis virus


organism: West Nile virus


organism: Yellow fever virus


organism: Avena sativa

substrate_name: biliverdin IXa

proof_lines:
above
Porphyra umbilicalis ferredoxin
Spinacia oleracea ferredoxin

substrate: biliverdin IXa


organism: Aspergillus flavipes

substrate_name: glutathione

substrate: glutathione


enzyme_display_name: NADPH--cytochrome-c2 reductase

organism: Cereibacter sphaeroides

enzyme: NADPH--cytochrome-c2 reductase


organism: Hapsidospora chrysogena

substrate_name: acetyl-CoA

assay_conditions_summary: cofactor CoA

substrate: acetyl-CoA


organism: Homo sapiens

substrate_name: lyso-phosphatidylserine

substrate: lyso-phosphatidylserine


organism: Oryctolagus cuniculus


organism: Rattus norvegicus


organism: Kluyveromyces lactis

substrate_name: UDP-N-acetylglucosamine

substrate: UDP-N-acetylglucosamine


proof_lines:
solubilized enzyme
membrane-bound enzyme


enzyme_display_name: monoterpenol beta-glucosyltransferase

organism: Mentha x piperita

substrate_name: UDPglucose

proof_lines:
reaction with D-neomenthol
reaction with L-menthol

enzyme: monoterpenol beta-glucosyltransferase

substrate: UDPglucose


substrate_name: GDPmannose

proof_lines: based on the assumption of incorporation of only a single mannosyl unit into the product

substrate: GDPmannose


organism: Sus scrofa


enzyme_display_name: nicotinate glucosyltransferase

organism: Petroselinum crispum

substrate_name: nicotinic acid

enzyme: nicotinate glucosyltransferase

substrate: nicotinic acid


enzyme_display_name: soyasapogenol glucuronosyltransferase

organism: Glycine max

substrate_name: soyasapogenol A

proof_lines: using soyasapogenol B as the sugar acceptor, in 100 mM Tris-HCl (pH 8.0), at 40°C

enzyme: soyasapogenol glucuronosyltransferase

substrate: soyasapogenol A


organism: Arabidopsis thaliana

substrate_name: UDP-xylose

proof_lines: pH 7.0, 37°C, recombinant mutant DELTA39 XylT

substrate: UDP-xylose


enzyme_display_name: diiodotyrosine transaminase

enzyme: diiodotyrosine transaminase


enzyme_display_name: uracilylalanine synthase

organism: Lathyrus oleraceus

substrate_name: Uracil

proof_lines:
willardiine synthesis
synthesis of isowillardiine

enzyme: uracilylalanine synthase

substrate: Uracil


enzyme_display_name: 4-hydroxyglutamate transaminase

enzyme: 4-hydroxyglutamate transaminase


enzyme_display_name: ADP--thymidine kinase

organism: Herpes simplex virus

substrate_name: thymidine

assay_conditions_summary: cofactor ADP

proof_lines:
Herpes simplex virus type 2
Herpes simplex virus type 1

enzyme: ADP--thymidine kinase

substrate: thymidine


enzyme_display_name: diphosphate--serine phosphotransferase

organism: Propionibacterium freudenreichii subsp. shermanii

substrate_name: diphosphate

proof_lines:
pH 7.8, 37°C
1 mM diphosphate, pH 7.8, 37°C

enzyme: diphosphate--serine phosphotransferase

substrate: diphosphate


enzyme_display_name: [heparan sulfate]-glucosamine 3-sulfotransferase 1

organism: Mus musculus

substrate_name: 3'-phosphoadenosine 5'-phosphosulfate

substrate: 3'-phosphoadenosine 5'-phosphosulfate


enzyme: [heparan sulfate]-glucosamine 3-sulfotransferase 1


organism: Zika virus

proof_lines: pH 8.5, 37°C, Tris-CHAPS-glycerol buffer

proof_lines: pH 8.5, temperature not specified in the publication


organism: Thermococcus kodakarensis

substrate_name: adenine9 in tRNAThr

proof_lines: pH 8.0, 50°C, recombinant enzyme, tRNA substrate from Thermococcus kodakarensis

proof_lines: pH 8.0, 40°C, recombinant enzyme, tRNA substrate from Saccharomyces cerevisiae

substrate: adenine9 in tRNAThr


organism: Bacteroides fragilis

substrate_name: N-succinyl-L-ornithine

substrate: N-succinyl-L-ornithine


enzyme_display_name: apulose-4-phosphate transketolase

organism: Actinobacillus succinogenes

substrate_name: D-Apulose 4-phosphate; D-Glyceraldehyde 3-phosphate

enzyme: apulose-4-phosphate transketolase


enzyme_display_name: acyl-Kdo2-lipid IVA acyltransferase

organism: Acinetobacter baumannii

substrate_name: hexadecanoyl-[acyl-carrier protein]

enzyme: acyl-Kdo2-lipid IVA acyltransferase

substrate: hexadecanoyl-[acyl-carrier protein]


organism: Oryza sativa

proof_lines: recombinant soluble enzyme, pH 6.0, 16°C


organism: Aspergillus fumigatus

substrate_name: dimethylallyl diphosphate

proof_lines: 37°C, pH not specified in the publication

substrate: dimethylallyl diphosphate


enzyme_display_name: O-phosphoseryl-tRNA(Sec) kinase

organism: Methanocaldococcus jannaschii

substrate_name: ATP

assay_conditions_summary: 310.15 K; cofactor ATP

enzyme: O-phosphoseryl-tRNA(Sec) kinase

substrate: ATP


organism: West Nile virus


enzyme_display_name: catalase peroxidase

organism: Mycobacterium tuberculosis


proof_lines: mutant enzyme I290V/Q293V, at pH 5.0 and 23°C


enzyme_display_name: quercetin-3-sulfate 3'-sulfotransferase

organism: Flaveria chlorifolia

enzyme: quercetin-3-sulfate 3'-sulfotransferase


organism: Chloroflexus aurantiacus

substrate_name: succinyl-CoA

assay_conditions_summary: 328.15 K; cofactor CoA

proof_lines: 55°C, pH and temperature not specified in the publication

substrate: succinyl-CoA


enzyme_display_name: ellagitannin acyl hydrolase

organism: Aspergillus niger

substrate_name: punicalagin

enzyme: ellagitannin acyl hydrolase

substrate: punicalagin


enzyme_display_name: 3-oxoadipate enol-lactonase

organism: Acinetobacter calcoaceticus

substrate_name: Unknown

enzyme: 3-oxoadipate enol-lactonase

substrate: Unknown


organism: Pseudomonas putida


enzyme_display_name: wax-ester hydrolase

organism: Simmondsia chinensis

substrate_name: N-methylindoxyl myristate

enzyme: wax-ester hydrolase

substrate: N-methylindoxyl myristate


organism: Sinapis alba

substrate_name: cholesteryl palmitate

substrate: cholesteryl palmitate


enzyme_display_name: 3-phosphoglycerate phosphatase

organism: Saccharum sp.

substrate_name: D-glycerate 3-phosphate

enzyme: 3-phosphoglycerate phosphatase

substrate: D-glycerate 3-phosphate


organism: Spinacia oleracea


enzyme_display_name: caldesmon-phosphatase

organism: Meleagris gallopavo

substrate_name: calponin

proof_lines: pH 7.5, 30°C, calponin phosphorylated by PKC

proof_lines: pH 7.5, 30°C, caldesmon phosphorylated by PKC cdc2

proof_lines: pH 7.5, 30°C, caldesmon phosphorylated by PKC CaM kinase II

enzyme: caldesmon-phosphatase

substrate: calponin


enzyme_display_name: D-arabinan exo alpha-(1,3)/(1,5)-arabinofuranosidase (non-reducing end)

organism: Dysgonomonas gadei

substrate_name: 4-nitrophenyl alpha-D-arabinofuranoside

substrate: 4-nitrophenyl alpha-D-arabinofuranoside


organism: Microbacterium arabinogalactanolyticum

enzyme: D-arabinan exo alpha-(1,3)/(1,5)-arabinofuranosidase (non-reducing end)


organism: Nocardia brasiliensis


enzyme_display_name: peptidyl-glycinamidase

organism: Rattus norvegicus

enzyme: peptidyl-glycinamidase


enzyme_display_name: oviductin

organism: Xenopus laevis

enzyme: oviductin


enzyme_display_name: DNA-3-methyladenine glycosylase I

organism: Escherichia coli

substrate_name: 3-methyladenine residues in alkylated DNA

proof_lines: pH 7.6, 37°C, release of 3-methyladenine

proof_lines: pH 7.8, 37°C, release of 3-methyladenine

enzyme: DNA-3-methyladenine glycosylase I


enzyme_display_name: licheninase

organism: Bacillus subtilis

substrate_name: H2O; Lichenin

enzyme: licheninase

substrate: H2O; Lichenin


organism: Streptococcus bovis

substrate_name: Lichenan; H2O

substrate: Lichenan; H2O


organism: Cupriavidus necator

substrate_name: 3-oxo-tetronate 4-phosphate

proof_lines: pH and temperature not specified in the publication

substrate: 3-oxo-tetronate 4-phosphate


organism: Haemophilus influenzae


organism: Pectobacterium atrosepticum


organism: Sulfolobus solfataricus

substrate_name: Pyruvate; D-Glyceraldehyde

substrate: Pyruvate; D-Glyceraldehyde


enzyme_display_name: xylose isomerase

organism: Actinoplanes missouriensis

substrate_name: D-glucose

enzyme: xylose isomerase

substrate: D-glucose


organism: Arthrobacter sp.

substrate_name: D-Xylose

substrate: D-Xylose


substrate_name: NAD+

assay_conditions_summary: 303.15 K; cofactor NADP+

proof_lines: cofactor NAD+, pH not specified in the publication, 30°C

proof_lines: cofactor NADP+, pH not specified in the publication, 30°C

substrate: NAD+


organism: Crocosphaera subtropica

substrate_name: NADP+

assay_conditions_summary: pH 9; 298.15 K; cofactor NADP+

proof_lines: pH 9.0, 25°C, recombinant wild-type enzyme, with NADP+

proof_lines: pH 9.0, 25°C, recombinant wild-type enzyme, with NAD+

substrate: NADP+


proof_lines: pH 9.0, 25°C, recombinant mutant S157E, with NADP+

proof_lines: pH 9.0, 25°C, recombinant mutant S157E, with NAD+


proof_lines: pH 9.0, 25°C, recombinant mutant K154A, with NADP+

proof_lines: pH 9.0, 25°C, recombinant mutant K154A, with NAD+


organism: Nitrosomonas europaea

assay_conditions_summary: pH 8.5; 303.15 K; cofactor NADP+

proof_lines: pH 8.5, 30°C, recombinant enzyme, with succinate semialdehyde

proof_lines: pH 8.5, 30°C, recombinant enzyme, with NADP+

proof_lines: pH 8.5, 30°C, recombinant enzyme, with NAD+


organism: Salmonella enterica subsp. enterica serovar Typhimurium

proof_lines: mutant enzyme K160E, in 50 mM HEPES (pH 8.0), at 37°C


substrate_name: succinate semialdehyde

assay_conditions_summary: pH 8; 310.15 K; cofactor NAD+

proof_lines: mutant enzyme F138A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C

substrate: succinate semialdehyde


proof_lines: mutant enzyme W141A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C


proof_lines: mutant enzyme K160A, in 50 mM HEPES (pH 8.0), at 37°C


assay_conditions_summary: pH 8; 310.15 K; cofactor NADP+

proof_lines: wild type enzyme, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C

proof_lines: wild type enzyme, in 50 mM HEPES (pH 8.0), at 37°C

proof_lines: wild type enzyme, with NADP+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C


organism: Saccharolobus solfataricus

substrate_name: KDPG (at 60 degrees Celsius and at pH 6)

proof_lines: kcat is 28.2 sec(-1) for KDG and 6.8 sec(-1) for KDGal.


organism: Cereibacter sphaeroides

assay_conditions_summary: cofactor NADP+


assay_conditions_summary: pH 6.5; 343.15 K; cofactor NADP+


organism: Euglena gracilis


organism: Klebsiella pneumoniae

assay_conditions_summary: pH 7.8; 303.15 K; cofactor NADP+


organism: Pseudomonas putida

assay_conditions_summary: pH 8.5; 303.15 K; buffer TAPS; cofactor NADP+

proof_lines: recombinant wild type enzyme, in TAPS buffer, at pH 8.5, at 30°C

proof_lines: native wild type enzyme, in TAPS buffer, at pH 8.5, at 30°C


assay_conditions_summary: pH 8; 303.15 K; cofactor NADP+

proof_lines: His-tagged BADH, cosubstrate benzaldehyde


organism: Mycobacterium tuberculosis

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NADP+

proof_lines: in the presence of 10 mM Mg2+, at pH 7.5 and 37°C

proof_lines: with NADP+ as cosubstrate, in the absence of Mg2+, at pH 7.5 and 37°C


organism: Rattus norvegicus

assay_conditions_summary: cofactor NAD+

proof_lines:
synaptosomes
mitochondria of non-synaptic origin


substrate_name: 2-oxoglutarate semialdehyde

proof_lines: with NAD+ as cosubstrate, at pH 6.5 and 70°C

proof_lines: with NADP+ as cosubstrate, at pH 6.5 and 70°C

substrate: 2-oxoglutarate semialdehyde


organism: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)

substrate_name: pyruvate (at pH 6 and at 70 degrees Celsius)

assay_conditions_summary: pH 6


organism: Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)

substrate: pyruvate (at pH 6 and at 70 degrees Celsius)


enzyme_display_name: N-benzyloxycarbonylglycine hydrolase

organism: Enterococcus faecalis

substrate_name: N-benzyl-Gly

enzyme: N-benzyloxycarbonylglycine hydrolase

substrate: N-benzyl-Gly


organism: Stutzerimonas stutzeri

assay_conditions_summary: pH 8.5; 298.15 K; cofactor NADP+

proof_lines: in the presence of benzaldehyde, at 25°C and pH 8.5

proof_lines: in the presence of NAD+, at 25°C and pH 8.5

proof_lines: in the presence of NADP+, at 25°C and pH 8.5


enzyme_display_name: CDP-diacylglycerol diphosphatase

organism: Cavia porcellus

substrate_name: CDPdipalmitoylglycerol

enzyme: CDP-diacylglycerol diphosphatase

substrate: CDPdipalmitoylglycerol


enzyme_display_name: bromoxynil nitrilase

organism: Klebsiella pneumoniae subsp. ozaenae

enzyme: bromoxynil nitrilase


organism: Piromyces sp.

substrate_name: D-xylose

assay_conditions_summary: pH 7.5; 303.15 K; buffer 100 mM Tris-HCl

proof_lines: mutant enzyme E129D/V433I/E15D/E114G/T142S/A177T, in 100 mM Tris-HCl buffer at pH 7.5 and 30°C

substrate: D-xylose


enzyme_display_name: N-carbamoylsarcosine amidase

organism: Arthrobacter sp.

substrate_name: N-carbamoylsarcosine

enzyme: N-carbamoylsarcosine amidase

substrate: N-carbamoylsarcosine


organism: Clostridium sp.


enzyme_display_name: D-lyxose ketol-isomerase

organism: Thermofilum sp. ex4484_79

substrate_name: D-Lyxose

enzyme: D-lyxose ketol-isomerase

substrate: D-Lyxose


organism: Thermoflavimicrobium dichotomicum

substrate_name: L-ribose

substrate: L-ribose


organism: Thermoprotei archaeon


organism: Thermosediminibacter oceani

substrate_name: D-fructose

substrate: D-fructose


enzyme_display_name: catalase peroxidase

organism: Archaeoglobus fulgidus

proof_lines: peroxidase activity, 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C

enzyme: catalase peroxidase


organism: Bacillus pumilus


organism: Burkholderia pseudomallei

proof_lines: mutant enzyme R108A/D141A, peroxidase activity, at pH 4.5 and 25°C

proof_lines: mutant enzyme R108A/D141A, catalase activity, at pH 7.0 and 37°C


proof_lines: mutant enzyme D141A, peroxidase activity, at pH 4.5 and 25°C

proof_lines: mutant enzyme D141A, catalase activity, at pH 7.0 and 37°C


proof_lines: mutant enzyme R108A, catalase activity, at pH 7.0 and 37°C

proof_lines: mutant enzyme R108A, peroxidase activity, at pH 4.5 and 25°C


proof_lines: mutant enzyme D141E, peroxidase activity, at pH 4.5 and 25°C

proof_lines: mutant enzyme D141E, catalase activity, at pH 7.0 and 37°C


proof_lines: mutant enzyme R108A/W111F/D141A, catalase activity, at pH 7.0 and 37°C

proof_lines: mutant enzyme R108A/W111F/D141A, peroxidase activity, at pH 4.5 and 25°C


proof_lines: mutant enzyme D141N, peroxidase activity, at pH 4.5 and 25°C

proof_lines: mutant enzyme D141N, catalase activity, at pH 7.0 and 37°C


proof_lines: mutant enzyme R108A/W111F, catalase activity, at pH 7.0 and 37°C

proof_lines: mutant enzyme R108A/W111F, peroxidase activity, at pH 4.5 and 25°C


proof_lines: wild type enzyme, peroxidase activity, at pH 4.5 and 25°C

proof_lines: wild type enzyme, catalase activity, at pH 7.0 and 37°C


proof_lines: mutant enzyme R108K, peroxidase activity, at pH 4.5 and 25°C

proof_lines: mutant enzyme R108K, catalase activity, at pH 7.0 and 37°C


organism: Mycobacterium tuberculosis

substrate_name: o-Dianisidine

proof_lines:
mutant enzyme N238S, at pH 4.5 and 30°C
mutant enzyme N238S, at pH 7.0 and 30°C

substrate: o-Dianisidine


enzyme_display_name: toluene 4-monooxygenase

organism: Burkholderia cepacia

substrate_name: methyl 4-tolyl sulfide

proof_lines: whole-cell assay, 25°C, pH not specified in the publication

enzyme: toluene 4-monooxygenase

substrate: methyl 4-tolyl sulfide


organism: Ectopseudomonas mendocina

substrate_name: Toluene

substrate: Toluene


proof_lines:
wild-type, pH 7.5, 25°C
wild-type, pH 7.5, 4°C


substrate_name: norcarane

proof_lines:
mutant T201A, pH 7.5, 25°C
mutant T201A, pH 7.5, 4°C

substrate: norcarane


enzyme_display_name: dichloromethane dehalogenase

organism: Methylophilus sp.

substrate_name: Dichloromethane

enzyme: dichloromethane dehalogenase

substrate: Dichloromethane


organism: Methylorubrum extorquens


organism: Methylorubrum rhodesianum


proof_lines: pH 8.0, 30°C, recombinant wild-type enzyme


organism: Niallia circulans


enzyme_display_name: L-Ala-D/L-Glu epimerase

organism: Bacillus subtilis

substrate_name: L-Alanyl-D-aspartate

enzyme: L-Ala-D/L-Glu epimerase

substrate: L-Alanyl-D-aspartate


substrate_name: L-Alanyl-D-glutamate

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

proof_lines:
pH 8.5, 37°C
Raw SKiD Env: Temp=37°C, pH=8.5

source_databases_merged: sabio_rk brenda skid

substrate: L-Alanyl-D-glutamate


substrate_name: L-Ala-D-Asp

substrate: L-Ala-D-Asp


enzyme_display_name: alpha,alpha-trehalose synthase

organism: Rubrobacter xylanophilus (strain DSM 9941 / JCM 11954 / NBRC 16129 / PRD-1)

substrate_name: ADP-glucose

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

assay_conditions_summary: cofactor ADP

enzyme: alpha,alpha-trehalose synthase

substrate: ADP-glucose


organism: Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C)


enzyme_display_name: fucokinase

organism: Arabidopsis thaliana

substrate_name: L-fucose for the L-fucokinase activity

assay_conditions_summary: cofactor ATP

enzyme: fucokinase

substrate: L-fucose for the L-fucokinase activity


enzyme_display_name: transaldolase

organism: Unknown

substrate_name: D-fructose 6-phosphate

enzyme: transaldolase

substrate: D-fructose 6-phosphate


enzyme_display_name: N-acylglucosamine 2-epimerase

organism: Anabaena sp. CH1

substrate_name: N-acetyl-D-glucosamine

proof_lines: E242Q, reverse conversion in the presence of 1 mM ATP

enzyme: N-acylglucosamine 2-epimerase

substrate: N-acetyl-D-glucosamine


substrate_name: N-acetyl-D-mannosamine

proof_lines: C370A, reverse conversion in the presence of 1 mM ATP

substrate: N-acetyl-D-mannosamine


proof_lines: R375K, reverse conversion in the presence of 1 mM ATP


proof_lines: E218D, reverse conversion in the presence of 1 mM ATP


proof_lines: E218A, reverse conversion in the presence of 1 mM ATP


proof_lines: R375A, reverse conversion in the presence of 1 mM ATP


organism: Homo sapiens

substrate_name: N-Acetyl-D-glucosamine

substrate: N-Acetyl-D-glucosamine


organism: Sus scrofa


organism: Trichormus variabilis

proof_lines: pH 7.5, 30°C, recombinant His6-tagged wild-type enzyme


proof_lines: pH 7.5, 30°C, recombinant His6-tagged mutant K160P


proof_lines: pH 7.5, 30°C, recombinant His6-tagged mutant K155A


organism: Bacillus methanolicus

sequence_source: UniProtKB accession metadata


organism: Blastobotrys adeninivorans


organism: Escherichia coli

proof_lines: mutant F189Y, reaction: sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate formation from erythrose 4-phosphate + D-fructose 6-phosphate

proof_lines: mutant F189Y, reaction: fructose 6-phosphate synthesis from dihydroxyacetone and glyceraldehyde 3-phosphate

proof_lines: mutant F189Y, reaction: fructose 6-phosphate cleavage into dihydroxyacetone and glyceraldehyde 3-phosphate


organism: Thermoplasma acidophilum


substrate_name: D-Fructose 6-phosphate

substrate: D-Fructose 6-phosphate


proof_lines: mutant enzyme E60Q/F132Y, at pH 7.5 and 30°C


organism: Thermotoga maritima

proof_lines:
pH 9.0, 80°C
Raw SKiD Env: Temp=80°C, pH=9


organism: Pyrococcus horikoshii

substrate_name: D-glucose

substrate: D-glucose


organism: Rubrobacter xylanophilus

assay_conditions_summary: 333.15 K; cofactor ADP


organism: Bacteroides fragilis

substrate_name: L-fucose

proof_lines: pH and temperature not specified in the publication

substrate: L-fucose


substrate_name: D-erythrose 4-phosphate

substrate: D-erythrose 4-phosphate


proof_lines: kcat is 0.39 sec(-1) for the fucokinase activity. kcat is 10.07 sec(-1) for the fucose-1-phosphate guanylyltransferase activity.


enzyme_display_name: stizolobate synthase

organism: Beta vulgaris

substrate_name: L-dopa

enzyme: stizolobate synthase

substrate: L-dopa


organism: Escherichia coli (strain K12)

substrate_name: L-DOPA

substrate: L-DOPA


enzyme_display_name: [protein]-arginine 3-hydroxylase

substrate_name: Arg-137 of RPS6

enzyme: [protein]-arginine 3-hydroxylase

substrate: Arg-137 of RPS6


organism: Streptomyces globisporus


organism: Xenorhabdus nematophila (strain ATCC 19061 / DSM 3370 / CCUG 14189 / LMG 1036 / NCIMB 9965 / AN6)

substrate_name: tyrosine isonitrile

substrate: tyrosine isonitrile


enzyme_display_name: levoglucosan dehydrogenase

organism: Pseudarthrobacter phenanthrenivorans (strain DSM 18606 / JCM 16027 / LMG 23796 / Sphe3)

substrate_name: levoglucosan (at pH 8.5 and 40 degrees Celsius)

proof_lines: kcat is 4.3 sec(-1) with levoglucosan as substrate. kcat is 26 sec(-1) with 3-dehydrolevoglucosan as substrate.

enzyme: levoglucosan dehydrogenase


enzyme_display_name: sulfide dehydrogenase

organism: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

substrate_name: NADPH (with benzyl viologen as cosubstrate at pH 9.5)

enzyme: sulfide dehydrogenase


substrate: NADPH (with benzyl viologen as cosubstrate at pH 9.5)


substrate_name: 16S rRNA

proof_lines: kcat is 0.012 sec(-1) with 16S rRNA as substrate.

substrate: 16S rRNA


organism: Streptomyces niveus

substrate_name: demethyldecarbamoylnovobiocin

proof_lines: kcat is 0.4 min(-1) with demethyldecarbamoylnovobiocin as substrate.

substrate: demethyldecarbamoylnovobiocin


substrate_name: D,L-glyceraldehyde 3-phosphate (with C3 acceptor compounds at pH 8.5 and 30 degrees Celsius)

proof_lines: kcat is 53 sec(-1) for D-fructose-6-phosphate.


organism: Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936)

proof_lines: kcat is 4114 min(-1) with D-erythrose 4-phosphate as substrate and 4151 min(-1) with D-fructose 6-phosphate as substrate.


enzyme_display_name: licheninase

organism: Bacillus amyloliquefaciens

substrate_name: H2O; 1,3-beta-D-Glucan

unit: l/(min*g)

enzyme: licheninase

substrate: H2O; 1,3-beta-D-Glucan


proof_lines: fusion protein encoding beta-1,3-1,4-glucanase both from Bacillus amyloliquefaciens and Clostridium thermocellum, pH 6.0, 70°C


organism: Bacillus licheniformis


enzyme_display_name: dipeptidyl-peptidase I

organism: Homo sapiens

substrate_name: Gly-Phe-4-methoxynaphthylamide; H2O

enzyme: dipeptidyl-peptidase I

substrate: Gly-Phe-4-methoxynaphthylamide; H2O


organism: Rattus norvegicus

substrate_name: Glycyl-Tyrosyl-amide; Hydroxylamine

substrate: Glycyl-Tyrosyl-amide; Hydroxylamine


organism: Methylophilus sp.


substrate_name: Dihalomethane; H2O

substrate: Dihalomethane; H2O


substrate_name: Dichloromethane

substrate: Dichloromethane


substrate_name: Dibromomethane

substrate: Dibromomethane


proof_lines:
30°C, pH 8.5, wild-type
pH 8.5, wild-type
pH 8.2, wild-type


organism: Ricinus communis

substrate_name: hexadecanoyl-[acyl-carrier protein]

proof_lines: wild type enzyme, pH and temperature not specified in the publication

substrate: hexadecanoyl-[acyl-carrier protein]


substrate_name: octadecanoyl-[acyl-carrier protein]

proof_lines: mutant enzyme T117R/G188L, pH and temperature not specified in the publication

substrate: octadecanoyl-[acyl-carrier protein]


substrate_name: Reduced ferredoxin; Stearoyl-[acyl-carrier protein]; O2


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: succinate semialdehyde

proof_lines: mutant enzyme D426A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C

substrate: succinate semialdehyde


proof_lines: mutant enzyme R428A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C


substrate_name: NAD+

proof_lines: mutant enzyme N193A, in 50 mM HEPES (pH 8.0), at 37°C

substrate: NAD+


proof_lines: mutant enzyme S425A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C


proof_lines: mutant enzyme R145A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C


proof_lines: mutant enzyme K92A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C


proof_lines: mutant enzyme E442A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C


organism: Streptococcus pyogenes

substrate_name: NADP+

assay_conditions_summary: pH 7; 303.15 K; cofactor NADP+

proof_lines: recombinant His6-tagged enzyme, pH 7.0, 30°C

substrate: NADP+


organism: Sulfurisphaera tokodaii

assay_conditions_summary: pH 8; 313.15 K; cofactor NADP+

proof_lines:
pH 8.0, 40°C, cosubstrate: NADP+
pH 8.0, 60°C
pH 8.0, 40°C, cosubstrate: NAD+


enzyme_display_name: 2-oxoglutarate synthase

organism: Hydrogenobacter thermophilus

substrate_name: CoA

assay_conditions_summary: pH 7.8; 343.15 K; cofactor CoA

enzyme: 2-oxoglutarate synthase

substrate: CoA


proof_lines: isozyme Kor, in 10 mM MOPS, pH 6.8, at 70°C


substrate_name: reduced ferredoxin

proof_lines: isozyme For, in 10 mM MOPS, pH 6.8, at 70°C

substrate: reduced ferredoxin


organism: Magnetococcus marinus

substrate_name: succinyl-CoA

assay_conditions_summary: pH 7; cofactor CoA

proof_lines: wild-type, pH 7.0, temperature not specified in the publication

proof_lines: wild-type, pH 8.5, temperature not specified in the publication

proof_lines: wild-type, pH 7.5, temperature not specified in the publication

substrate: succinyl-CoA


proof_lines: mutant Y436F, pH 7.5, temperature not specified in the publication


proof_lines: mutant E45Q, pH 7.5, temperature not specified in the publication


organism: Thauera aromatica

assay_conditions_summary: pH 7.8; 303.15 K; cofactor CoA


enzyme_display_name: 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH)

organism: Chlorobaculum tepidum

substrate_name: 3,8-divinyl chlorophyllide a

proof_lines: recombinant BciA, pH and temperature not specified in the publication

substrate: 3,8-divinyl chlorophyllide a


organism: Rhodobacter capsulatus

proof_lines: pH 8.0, 22°C, recombinant Strep II-tagged oligomeric enzyme, low salt condition

proof_lines: pH 8.0, 22°C, recombinant Strep II-tagged dimeric enzyme, low salt condition

proof_lines: pH 8.0, 22°C, recombinant Strep II-tagged dimeric enzyme, high salt condition

enzyme: 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH)


enzyme_display_name: L-alanine--L-anticapsin ligase

organism: Bacillus subtilis

substrate_name: L-alanine

enzyme: L-alanine--L-anticapsin ligase

substrate: L-alanine


proof_lines:
pH 7.7, 37°C, mutant enzyme Y75F
pH 7.1, 37°C, mutant enzyme Y75F


proof_lines:
pH 7.7, 37°C, wild-type enzyme
pH 7.1, 37°C, wild-type enzyme


substrate_name: L-Alanyl-D-methionine

substrate: L-Alanyl-D-methionine


substrate_name: L-Ala-D-Glu

substrate: L-Ala-D-Glu


substrate_name: L-Ala-D-Met

substrate: L-Ala-D-Met


organism: Escherichia coli


substrate_name: L-Ala-D-Gln

substrate: L-Ala-D-Gln


substrate_name: L-Ala-D-Asp

substrate: L-Ala-D-Asp


organism: Anabaena sp. CH1

substrate_name: N-acetyl-D-mannosamine

proof_lines: N179A, reverse conversion in the presence of 1 mM ATP

substrate: N-acetyl-D-mannosamine


assay_conditions_summary: pH 7; cofactor ATP

proof_lines: wild type, at pH 7, in the presence of 1 mM ATP

proof_lines: wild type, at pH 8, in the presence of 1 mM ATP

proof_lines: wild type, at pH 9, in the presence of 1 mM ATP


organism: Trichormus variabilis

substrate_name: N-acetyl-D-glucosamine

proof_lines: pH 7.5, 30°C, recombinant His6-tagged mutant K151V

substrate: N-acetyl-D-glucosamine


proof_lines: pH 7.5, 30°C, recombinant His6-tagged mutant K157L


enzyme_display_name: D-lyxose ketol-isomerase

organism: Bacillus licheniformis

substrate_name: D-mannose

proof_lines: in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C

enzyme: D-lyxose ketol-isomerase

substrate: D-mannose


organism: Bacillus velezensis

substrate_name: D-Lyxose

substrate: D-Lyxose


organism: Cohnella laeviribosi


organism: Dictyoglomus turgidum

substrate_name: L-ribulose

proof_lines:
at pH 7.5 and 75°C
pH 7.5, 75°C

substrate: L-ribulose


organism: Providencia stuartii

substrate_name: D-xylulose

substrate: D-xylulose


organism: Serratia proteamaculans

proof_lines: at pH 7.5 and 40°C, in the presence of 1 mM Mn2+


substrate_name: ATP; L-Alanine; L-Glutamine

assay_conditions_summary: pH 9; 310.15 K; cofactor ATP

proof_lines:
pH 9.0, 37°C
pH 7.7, 37°C
Raw SKiD Env: Temp=37°C, pH=7.1

substrate: ATP; L-Alanine; L-Glutamine


substrate_name: L-phenylalanine

proof_lines:
pH 7.7, 37°C, mutant enzyme
pH 7.1, 37°C, mutant enzyme

substrate: L-phenylalanine


enzyme_display_name: catalase peroxidase

organism: Mycobacterium tuberculosis

enzyme: catalase peroxidase


proof_lines: mutant enzyme I290A/Q293A, at pH 5.0 and 23°C


enzyme_display_name: flavivirin

organism: Alkhumra hemorrhagic fever virus

enzyme: flavivirin


substrate_name: D-fructose 6-phosphate

substrate: D-fructose 6-phosphate


substrate_name: glyceraldehyde 3-phosphate

proof_lines:
wild-type, pH 8.5, 30°C
wild-type
wild-type, N35A

substrate: glyceraldehyde 3-phosphate


substrate_name: D-glyceraldehyde

substrate: D-glyceraldehyde


substrate_name: 3,8-divinyl protochlorophyllide a

proof_lines: oligomeric enzyme (low salt condition, 150 mM NaCl), at pH 8.0 and 25°C

proof_lines: pH and temperature not specified in the publication

proof_lines: dimeric enzyme (low salt condition, 150 mM NaCl), at pH 8.0 and 25°C

substrate: 3,8-divinyl protochlorophyllide a


organism: Hydrogenobacter thermophilus

assay_conditions_summary: pH 7.6; 343.15 K; cofactor CoA


organism: Prosthecochloris vibrioformis f. thiosulfatophilum

substrate_name: succinyl-CoA

assay_conditions_summary: cofactor CoA

substrate: succinyl-CoA


organism: Thermococcus litoralis

substrate_name: oxidized ferredoxin

assay_conditions_summary: pH 8.4; 353.15 K; cofactor CoA

proof_lines: pH 8.4, 80°C, ferredoxin from Pyrococcus furiosus

substrate: oxidized ferredoxin


organism: Arthrobacter sp.


organism: Blastobotrys adeninivorans


organism: Bos taurus

substrate_name: D-erythrose 4-phosphate

substrate: D-erythrose 4-phosphate


organism: Cyberlindnera jadinii


organism: Dictyostelium discoideum


organism: Escherichia coli

proof_lines: transaldolase B, competitive inhibition with respect to D-erythrose 4-phosphate, at pH 7.2 and 37°C


organism: Fusarium oxysporum


organism: Homo sapiens

proof_lines: mutant F189Y, reaction: fructose 6-phosphate cleavage into dihydroxyacetone and glyceraldehyde 3-phosphate

proof_lines: mutant F189Y, reaction: sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate formation from erythrose 4-phosphate + D-fructose 6-phosphate

proof_lines: mutant F189Y, reaction: fructose 6-phosphate synthesis from dihydroxyacetone and glyceraldehyde 3-phosphate


organism: Pyrococcus horikoshii

substrate_name: UDP

assay_conditions_summary: pH 6; 333.15 K; cofactor ADP

substrate: UDP


assay_conditions_summary: pH 6.5; 363.15 K; cofactor ADP


organism: Thermoproteus tenax

substrate_name: UDP-glucose

proof_lines: in 50 mM HEPES/KOH (pH 7.0), with 20 mM MgCl2, at 70°C

substrate: UDP-glucose


organism: Thermotoga maritima

substrate_name: GDP-alpha-D-glucose

substrate: GDP-alpha-D-glucose


organism: Methanocaldococcus jannaschii

substrate_name: D-ribose 5-phosphate

proof_lines: pH 7.5, 50°C, concentration of ribose-5-phosphate between 0.5 and 15 mM

substrate: D-ribose 5-phosphate


organism: Saccharomyces cerevisiae

substrate_name: sedoheptulose 7-phosphate

substrate: sedoheptulose 7-phosphate


organism: Solanum lycopersicum

substrate_name: erythrose 4-phosphate

substrate: erythrose 4-phosphate


organism: Rattus norvegicus

substrate_name: Unknown

substrate: Unknown


organism: Vitis vinifera

substrate_name: ATP

substrate: ATP


organism: Arabidopsis thaliana

substrate_name: L-fucose

assay_conditions_summary: pH 7.5; 308.15 K; cofactor ATP

substrate: L-fucose


organism: Bacteroides fragilis

substrate_name: L-galactose

substrate: L-galactose


organism: Canis lupus familiaris

assay_conditions_summary: pH 6.5; 310.15 K; cofactor ATP


organism: Mortierella alpina


enzyme_display_name: dichloromethane dehalogenase

organism: Niallia circulans

substrate_name: Dichloromethane

enzyme: dichloromethane dehalogenase

substrate: Dichloromethane


organism: Hyphomicrobium sp.


organism: Hyphomicrobium sp. DM2


organism: Methylobacterium sp.


organism: Methylorubrum extorquens DM4


organism: Synechocystis sp.

substrate_name: N-acetyl-D-mannosamine

substrate: N-acetyl-D-mannosamine


organism: Trichormus variabilis

substrate_name: N-acetyl-D-glucosamine

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

substrate: N-acetyl-D-glucosamine


organism: Homo sapiens


organism: Nostoc punctiforme


organism: Nostoc sp.


organism: Sus scrofa

assay_conditions_summary: cofactor ATP

proof_lines: in presence of saturating concentrations of ATP


substrate_name: ATP; L-Fucose

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

proof_lines:
pH 7.4, 37°C
pH 8.0, 37°C
ATP in form of MgATP2-

substrate: ATP; L-Fucose


organism: Caldanaerobius polysaccharolyticus

substrate_name: L-ribose

substrate: L-ribose


organism: Klebsiella aerogenes

substrate_name: mannose

substrate: mannose


organism: Paenibacillus baekrokdamisoli

substrate_name: D-allulose

assay_conditions_summary: pH 8

substrate: D-allulose


organism: Providencia stuartii

substrate_name: D-mannose

assay_conditions_summary: pH 7.5; 318.15 K; buffer 50 mM HEPES

substrate: D-mannose


organism: Bacillus thuringiensis

substrate_name: H2O; D-myo-Inositol 1,2-cyclic phosphate

sequence_source: UniProtKB:P08954 (active entry; sequence exists but is not emitted in this lane)


organism: Bacillus cereus

proof_lines: pH 7, 25°C, in presence of dihexanoylphosphatidylcholine

proof_lines: pH 7, 25°C, in absence of dihexanoylphosphatidylcholine


organism: Listeria monocytogenes

assay_conditions_summary: pH 7


enzyme_display_name: gluconate 2-dehydrogenase (acceptor)

organism: Serratia marcescens

substrate_name: D-gluconate

enzyme: gluconate 2-dehydrogenase (acceptor)

substrate: D-gluconate


organism: Gluconobacter oxydans


organism: Pseudomonas aeruginosa

assay_conditions_summary: pH 5


organism: Pseudomonas fluorescens

assay_conditions_summary: pH 6


enzyme_display_name: farnesyl diphosphatase

organism: Saccharolobus solfataricus

substrate_name: geranylgeranyl diphosphate

proof_lines:
pH 7, 37°C
pH 7, 60°C

enzyme: farnesyl diphosphatase

substrate: geranylgeranyl diphosphate


organism: Cricetulus griseus

substrate_name: (2E,6E)-farnesyl diphosphate

substrate: (2E,6E)-farnesyl diphosphate


organism: Oryza sativa


organism: Rattus norvegicus


organism: Bacillus subtilis (strain 168)

substrate_name: farnesyl pyrophosphate

substrate: farnesyl pyrophosphate


organism: Zea mays

substrate_name: geranyl diphosphate

substrate: geranyl diphosphate


organism: Homo sapiens

sequence_source: UniProtKB:P51878 accession-anchor check: reviewed human caspase-5 entry with a sequence present; sequence not adopted in this lane.


enzyme_display_name: N-carbamoylputrescine amidase

organism: Arabidopsis thaliana

substrate_name: H2O; N-Carbamoylputrescine

enzyme: N-carbamoylputrescine amidase

substrate: H2O; N-Carbamoylputrescine


substrate_name: N-Carbamoylputrescine

proof_lines: recombinant enzyme, pH and temperature not specified in the publication

substrate: N-Carbamoylputrescine


organism: Bacteroides thetaiotaomicron


organism: Selenomonas ruminantium


enzyme_display_name: tRNA(Met) cytidine acetyltransferase

organism: Escherichia coli

substrate_name: ATP; H2O

enzyme: tRNA(Met) cytidine acetyltransferase

substrate: ATP; H2O


enzyme_display_name: allantoate deiminase

organism: Glycine max

substrate_name: H2O; Allantoate

enzyme: allantoate deiminase

substrate: H2O; Allantoate


enzyme_display_name: [amino-group carrier protein]--L-2-aminoadipate ligase

organism: Thermus thermophilus


enzyme: [amino-group carrier protein]--L-2-aminoadipate ligase


enzyme_display_name: galactose-6-phosphate isomerase

organism: Lactococcus lactis subsp. lactis

substrate_name: D-Tagatose 6-phosphate

enzyme: galactose-6-phosphate isomerase

substrate: D-Tagatose 6-phosphate


organism: Lactococcus lactis

substrate_name: D-allose

substrate: D-allose


organism: Staphylococcus aureus

substrate_name: D-tagatose 6-phosphate

substrate: D-tagatose 6-phosphate


enzyme_display_name: methylmalonyl-CoA epimerase

enzyme: methylmalonyl-CoA epimerase


organism: Cereibacter sphaeroides


organism: Pyrococcus horikoshii

substrate_name: methylmalonyl-CoA

substrate: methylmalonyl-CoA


enzyme_display_name: avenacosidase

organism: Avena sativa

substrate_name: avenacoside B

enzyme: avenacosidase

substrate: avenacoside B


substrate_name: p-nitrophenyl-beta-D-glucopyranoside (with native enzyme)

proof_lines: The activity increases with rising aggregation of the enzyme. kcat is 982000 sec(-1) with avenacosides as substrate. kcat is 3090 sec(-1) with p-nitrophenyl-beta-D-glucopyranoside as substrate. kcat is 290 sec(-1) with genistin as substrate


substrate_name: N-carbamoylputrescine

substrate: N-carbamoylputrescine


substrate_name: p-nitrophenyl-beta-D-glucopyranoside (with heteromultimeric recombinant enzyme)


enzyme_display_name: thermospermine synthase

substrate_name: S-Adenosylmethioninamine; Spermidine

sequence_source: UniProt Q5SK28 accession context checked directly; no row-level sequence payload is established.

enzyme: thermospermine synthase

substrate: S-Adenosylmethioninamine; Spermidine


enzyme_display_name: sedoheptulokinase

organism: Mus musculus

substrate_name: ATP; Sedoheptulose

sequence_source: UniProt Q9D5J6 accession context checked directly; no row-level sequence payload is established.

enzyme: sedoheptulokinase

substrate: ATP; Sedoheptulose


enzyme_display_name: (3S)-malyl-CoA thioesterase

organism: Haloarcula marismortui

substrate_name: L-Malyl-CoA; H2O

sequence_source: UniProt Q5V463 accession context checked directly; no row-level sequence payload is established.

enzyme: (3S)-malyl-CoA thioesterase

substrate: L-Malyl-CoA; H2O


sequence_source: UniProt Q5V0X0 accession context checked directly; no row-level sequence payload is established.


organism: Rhodobacter sphaeroides

sequence_source: UniProt D3JV05 accession context checked directly; no row-level sequence payload is established.


substrate_name: H2O; p-Nitrophenyl-beta-D-glucoside

sequence_source: UniProt Q9ZP27 and Q38786 accession context checked directly; no row-level sequence payload is established.

substrate: H2O; p-Nitrophenyl-beta-D-glucoside


enzyme_display_name: ADP-glyceromanno-heptose 6-epimerase

organism: Unknown

substrate_name: ADP-D-glycero-D-manno-heptose

assay_conditions_summary: cofactor ADP

enzyme: ADP-glyceromanno-heptose 6-epimerase

substrate: ADP-D-glycero-D-manno-heptose


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: 3,4-DHSA (at pH 8 and at 25 degrees Celsius)