column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Oxacillin' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 1750 +/- 20; column 'kcat (s-1)' = 325; column 'kcat/Km (M-1 s-1)' = 1.90 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 1750 uM; 1750 / 1000 = 1.75 mM; the exported value is 1.75 mM. kcat: printed Table 3 cell 325 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 325 s^(-1). kcat/Km: printed Table 3 cell 1.90 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 190000 / 1000 = 190 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Oxacillin': Km = 1750 +/- 20 uM; kcat = 325 s^-1; printed kcat/Km = 1.90 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Oxacillin' within the 'Bacillus cereus beta-lactamase II' column group


enzyme_display_name: beta-lactamase

organism: Bacillus cereus

substrate_name: Cephalexin

verification_status: verified

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

protein_accession_database: UniProt

source_license: as published (see paper)

km_unit: mM

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cephalexin' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 300 +/- 26; column 'kcat (s-1)' = 1.7; column 'kcat/Km (M-1 s-1)' = 5.70 x 10^3.

normalized_value_unit_evidence: Km: printed Table 3 cell 300 uM; 300 / 1000 = 0.3 mM; the exported value is 0.3 mM. kcat: printed Table 3 cell 1.7 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 1.7 s^(-1). kcat/Km: printed Table 3 cell 5.70 x 10^3 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 5700 / 1000 = 5.7 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Cephalexin': Km = 300 +/- 26 uM; kcat = 1.7 s^-1; printed kcat/Km = 5.70 x 10^3 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cephalexin' within the 'Bacillus cereus beta-lactamase II' column group

table_label: Table 3: Kinetic parameters determined for class B beta-lactamases (Biochemical Journal 1993, 291:151-155)

enzyme: beta-lactamase

substrate: Cephalexin

status: verified


substrate_name: Cephaloglycin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cephaloglycin' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 190 +/- 5; column 'kcat (s-1)' = 61; column 'kcat/Km (M-1 s-1)' = 3.20 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 190 uM; 190 / 1000 = 0.19 mM; the exported value is 0.19 mM. kcat: printed Table 3 cell 61 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 61 s^(-1). kcat/Km: printed Table 3 cell 3.20 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 320000 / 1000 = 320 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Cephaloglycin': Km = 190 +/- 5 uM; kcat = 61 s^-1; printed kcat/Km = 3.20 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cephaloglycin' within the 'Bacillus cereus beta-lactamase II' column group

substrate: Cephaloglycin


substrate_name: Nitrocefin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Nitrocefin' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 70 +/- 5; column 'kcat (s-1)' = 45; column 'kcat/Km (M-1 s-1)' = 6.40 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 70 uM; 70 / 1000 = 0.07 mM; the exported value is 0.07 mM. kcat: printed Table 3 cell 45 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 45 s^(-1). kcat/Km: printed Table 3 cell 6.40 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 640000 / 1000 = 640 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Nitrocefin': Km = 70 +/- 5 uM; kcat = 45 s^-1; printed kcat/Km = 6.40 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Nitrocefin' within the 'Bacillus cereus beta-lactamase II' column group

substrate: Nitrocefin


substrate_name: Cefuroxime

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cefuroxime' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 45 +/- 5; column 'kcat (s-1)' = 35; column 'kcat/Km (M-1 s-1)' = 7.80 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 45 uM; 45 / 1000 = 0.045 mM; the exported value is 0.045 mM. kcat: printed Table 3 cell 35 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 35 s^(-1). kcat/Km: printed Table 3 cell 7.80 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 780000 / 1000 = 780 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Cefuroxime': Km = 45 +/- 5 uM; kcat = 35 s^-1; printed kcat/Km = 7.80 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cefuroxime' within the 'Bacillus cereus beta-lactamase II' column group

substrate: Cefuroxime


substrate_name: Cefotaxime

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cefotaxime' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 90 +/- 10; column 'kcat (s-1)' = 60; column 'kcat/Km (M-1 s-1)' = 6.70 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 90 uM; 90 / 1000 = 0.09 mM; the exported value is 0.09 mM. kcat: printed Table 3 cell 60 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 60 s^(-1). kcat/Km: printed Table 3 cell 6.70 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 670000 / 1000 = 670 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Cefotaxime': Km = 90 +/- 10 uM; kcat = 60 s^-1; printed kcat/Km = 6.70 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cefotaxime' within the 'Bacillus cereus beta-lactamase II' column group

substrate: Cefotaxime


substrate_name: Sulbactam

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Sulbactam' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 5200 +/- 325; column 'kcat (s-1)' = 10; column 'kcat/Km (M-1 s-1)' = 1.90 x 10^3.

normalized_value_unit_evidence: Km: printed Table 3 cell 5200 uM; 5200 / 1000 = 5.2 mM; the exported value is 5.2 mM. kcat: printed Table 3 cell 10 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 10 s^(-1). kcat/Km: printed Table 3 cell 1.90 x 10^3 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1900 / 1000 = 1.9 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Sulbactam': Km = 5200 +/- 325 uM; kcat = 10 s^-1; printed kcat/Km = 1.90 x 10^3 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Sulbactam' within the 'Bacillus cereus beta-lactamase II' column group

substrate: Sulbactam


substrate_name: Cefoxitin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cefoxitin' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 2100 +/- 465; column 'kcat (s-1)' = 0.2; column 'kcat/Km (M-1 s-1)' = 9.50 x 10^1.

normalized_value_unit_evidence: Km: printed Table 3 cell 2100 uM; 2100 / 1000 = 2.1 mM; the exported value is 2.1 mM. kcat: printed Table 3 cell 0.2 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.2 s^(-1). kcat/Km: printed Table 3 cell 9.50 x 10^1 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 95 / 1000 = 0.095 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Cefoxitin': Km = 2100 +/- 465 uM; kcat = 0.2 s^-1; printed kcat/Km = 9.50 x 10^1 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cefoxitin' within the 'Bacillus cereus beta-lactamase II' column group

substrate: Cefoxitin


substrate_name: Moxalactam

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Moxalactam' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 644 +/- 90; column 'kcat (s-1)' = 1.2; column 'kcat/Km (M-1 s-1)' = 1.90 x 10^3.

normalized_value_unit_evidence: Km: printed Table 3 cell 644 uM; 644 / 1000 = 0.644 mM; the exported value is 0.644 mM. kcat: printed Table 3 cell 1.2 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 1.2 s^(-1). kcat/Km: printed Table 3 cell 1.90 x 10^3 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1900 / 1000 = 1.9 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Moxalactam': Km = 644 +/- 90 uM; kcat = 1.2 s^-1; printed kcat/Km = 1.90 x 10^3 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Moxalactam' within the 'Bacillus cereus beta-lactamase II' column group

substrate: Moxalactam


organism: Mycobacterium tuberculosis

substrate_name: Cephaloridine

assay_conditions_summary: pH 6; 310.15 K; buffer 0.1 M sodium phosphate

column_label: Table 3; measurement recombinant Y49 b-lactamase cephaloridine row; construct recombinant Y49 b-lactamase; substrate cephaloridine; assayed in 0.1 M sodium phosphate buffer pH 6.0 at 37 C. kcat 8 s^-1. Km 798 uM. Relative Vmax 39. REH 2. No kcat/Km cell is printed in Table 3.

normalized_value_unit_evidence: Km: printed Table 3 cell 798 uM; 798 / 1000 = 0.798 mM; the exported value is 0.798 mM. kcat: printed Table 3 cell 8 s^-1; the printed unit is already s^-1, so no unit conversion applies; the exported value is 8.0 s^(-1).

raw_value_unit_evidence: Table 3, recombinant Y-49 b-lactamase block, cephaloridine row: Km = 798 uM; kcat = 8 s^-1; Relative Vmax = 39; REH = 2. Table 3 prints no catalytic-efficiency column and the Ki column is blank on this line.

table_label: Table 3: Kinetic characteristics of recombinant Y49 b-lactamase from E. coli and b-lactamase obtained directly from M. tuberculosis (Antimicrob. Agents Chemother. 1998, 42(6):1375-1381)

substrate: Cephaloridine


substrate_name: Cefazolin

column_label: Table 3; measurement recombinant Y49 b-lactamase cefazolin row; construct recombinant Y49 b-lactamase; substrate cefazolin; assayed in 0.1 M sodium phosphate buffer pH 6.0 at 37 C. kcat 8 s^-1. Km 490 uM. Relative Vmax 36. REH 4. No kcat/Km cell is printed in Table 3.

normalized_value_unit_evidence: Km: printed Table 3 cell 490 uM; 490 / 1000 = 0.49 mM; the exported value is 0.49 mM. kcat: printed Table 3 cell 8 s^-1; the printed unit is already s^-1, so no unit conversion applies; the exported value is 8.0 s^(-1).

raw_value_unit_evidence: Table 3, recombinant Y-49 b-lactamase block, cefazolin row: Km = 490 uM; kcat = 8 s^-1; Relative Vmax = 36; REH = 4. Table 3 prints no catalytic-efficiency column and the Ki column is blank on this line.

substrate: Cefazolin


substrate_name: Cephapirin

column_label: Table 3; measurement recombinant Y49 b-lactamase cephapirin row; construct recombinant Y49 b-lactamase; substrate cephapirin; assayed in 0.1 M sodium phosphate buffer pH 6.0 at 37 C. kcat 3 s^-1. Km 680 uM. Relative Vmax 15. REH 1. No kcat/Km cell is printed in Table 3.

normalized_value_unit_evidence: Km: printed Table 3 cell 680 uM; 680 / 1000 = 0.68 mM; the exported value is 0.68 mM. kcat: printed Table 3 cell 3 s^-1; the printed unit is already s^-1, so no unit conversion applies; the exported value is 3.0 s^(-1).

raw_value_unit_evidence: Table 3, recombinant Y-49 b-lactamase block, cephapirin row: Km = 680 uM; kcat = 3 s^-1; Relative Vmax = 15; REH = 1. Table 3 prints no catalytic-efficiency column and the Ki column is blank on this line.

substrate: Cephapirin


substrate_name: Cefamandole

column_label: Table 3; measurement recombinant Y49 b-lactamase cefamandole row; construct recombinant Y49 b-lactamase; substrate cefamandole; assayed in 0.1 M sodium phosphate buffer pH 6.0 at 37 C. kcat 22 s^-1. Km 645 uM. Relative Vmax 104. REH 8. No kcat/Km cell is printed in Table 3.

normalized_value_unit_evidence: Km: printed Table 3 cell 645 uM; 645 / 1000 = 0.645 mM; the exported value is 0.645 mM. kcat: printed Table 3 cell 22 s^-1; the printed unit is already s^-1, so no unit conversion applies; the exported value is 22.0 s^(-1).

raw_value_unit_evidence: Table 3, recombinant Y-49 b-lactamase block, cefamandole row: Km = 645 uM; kcat = 22 s^-1; Relative Vmax = 104; REH = 8. Table 3 prints no catalytic-efficiency column and the Ki column is blank on this line.

substrate: Cefamandole


organism: Citrobacter diversus

column_label: Table 4 (Substrate profile of C. diversus beta-lactamases), 'Form I' group, substrate row 'Cephaloridine'; column 'Km (uM)' = 740 +/- 70; column 'kcat (s-1)' = 930 +/- 100; column 'kcat/Km (M-1 s-1)' = (1.25 +/- 0.25) x 10^6.

normalized_value_unit_evidence: Km: printed Table 4 cell 740 +/- 70 uM; 740 / 1000 = 0.74 mM; the exported value is 0.74 mM. kcat: printed Table 4 cell 930 +/- 100 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 930 s^(-1). kcat/Km: printed Table 4 cell (1.25 +/- 0.25) x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1250000 / 1000 = 1250 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 4, 'Form I' group, substrate row 'Cephaloridine': Km = 740 +/- 70 uM; kcat = 930 +/- 100 s^-1; printed kcat/Km = (1.25 +/- 0.25) x 10^6 M^-1 s^-1.

table_label: Table 4: Substrate profile of C. diversus beta-lactamases (Biochemical Journal 1988, 254:885-890)


substrate_name: Cephalothin

column_label: Table 4 (Substrate profile of C. diversus beta-lactamases), 'Form I' group, substrate row 'Cephalothin'; column 'Km (uM)' = 48 +/- 3; column 'kcat (s-1)' = 63 +/- 3; column 'kcat/Km (M-1 s-1)' = (1.3 +/- 0.15) x 10^6.

normalized_value_unit_evidence: Km: printed Table 4 cell 48 +/- 3 uM; 48 / 1000 = 0.048 mM; the exported value is 0.048 mM. kcat: printed Table 4 cell 63 +/- 3 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 63 s^(-1). kcat/Km: printed Table 4 cell (1.3 +/- 0.15) x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1300000 / 1000 = 1300 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 4, 'Form I' group, substrate row 'Cephalothin': Km = 48 +/- 3 uM; kcat = 63 +/- 3 s^-1; printed kcat/Km = (1.3 +/- 0.15) x 10^6 M^-1 s^-1.

substrate: Cephalothin


substrate_name: Cephalosporin C

column_label: Table 4 (Substrate profile of C. diversus beta-lactamases), 'Form I' group, substrate row 'Cephalosporin C'; column 'Km (uM)' = 126 +/- 20; column 'kcat (s-1)' = 41 +/- 5; column 'kcat/Km (M-1 s-1)' = (0.3 +/- 0.06) x 10^6.

normalized_value_unit_evidence: Km: printed Table 4 cell 126 +/- 20 uM; 126 / 1000 = 0.126 mM; the exported value is 0.126 mM. kcat: printed Table 4 cell 41 +/- 5 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 41 s^(-1). kcat/Km: printed Table 4 cell (0.3 +/- 0.06) x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 300000 / 1000 = 300 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 4, 'Form I' group, substrate row 'Cephalosporin C': Km = 126 +/- 20 uM; kcat = 41 +/- 5 s^-1; printed kcat/Km = (0.3 +/- 0.06) x 10^6 M^-1 s^-1.

substrate: Cephalosporin C


enzyme_display_name: L-iditol 2-dehydrogenase

organism: Bos taurus

substrate_name: D-Fructose

verification_status: unverified

enzyme: L-iditol 2-dehydrogenase

substrate: D-Fructose

status: unverified


substrate_name: NADH

substrate: NADH


enzyme_display_name: aldehyde dehydrogenase (NAD(+))

organism: Homo sapiens

substrate_name: formaldehyde

smiles: C=O

enzyme: aldehyde dehydrogenase (NAD(+))

substrate: formaldehyde


substrate_name: acetaldehyde

smiles: CC=O

substrate: acetaldehyde


substrate_name: propanal

smiles: CCC=O

substrate: propanal


substrate_name: pentanal

smiles: CCCCC=O

substrate: pentanal


substrate_name: hexanal

smiles: CCCCCC=O

substrate: hexanal


substrate_name: heptanal

smiles: CCCCCCC=O

substrate: heptanal


substrate_name: decanal

smiles: CCCCCCCCCC=O

substrate: decanal


substrate_name: p-nitrocinnamaldehyde

substrate: p-nitrocinnamaldehyde


substrate_name: p-(dimethylamino)cinnamaldehyde

substrate: p-(dimethylamino)cinnamaldehyde


substrate_name: trans-cinnamaldehyde

substrate: trans-cinnamaldehyde


substrate_name: hydrocinnamaldehyde

substrate: hydrocinnamaldehyde


substrate_name: alpha-phenylpropionaldehyde

substrate: alpha-phenylpropionaldehyde


substrate_name: phenylacetaldehyde

substrate: phenylacetaldehyde


substrate_name: benzaldehyde

substrate: benzaldehyde


enzyme_display_name: 4-hydroxybenzoyl-CoA thioesterase

organism: Pseudomonas sp.

enzyme: 4-hydroxybenzoyl-CoA thioesterase


substrate_name: benzylpenicillin

assay_conditions_summary: pH 7; 293.15 K; buffer containing 0

column_label: Table 1: Rate constants for the hydrolysis of benzylpenicillin (Biochem. J. 1990, 272:613-619), wild-type column, substrate row 'benzylpenicillin'; column 'Km(UM), decoded as Km in uM' = 65; column 'kcat. (s-1)' = 2200; column 'kcat./Km (j#m1 s-.), decoded as kcat/Km in uM^-1 s^-1' = 34.

normalized_value_unit_evidence: Km: printed cell 65 uM; 65 / 1000 = 0.065 mM. kcat: printed cell 2200 s^-1; already in s^-1, no unit conversion applies, so the cell is 2200.0 s^(-1). kcat/Km: printed cell 34 uM^-1 s^-1; 1 uM^-1 s^-1 is 1000 s^-1 mM^-1, so 34 x 1000 = 34000.0 s^-1 mM^-1.

raw_value_unit_evidence: Table 1. Rate constants for the hydrolysis of benzylpenicillin, wild-type column, substrate row 'benzylpenicillin': Km = 65 uM; kcat = 2200 s^-1; printed kcat/Km = 34 uM^-1 s^-1.

row_label: Table 1, wild-type column, hydrolysis of benzylpenicillin

substrate: benzylpenicillin


organism: Ovis aries

substrate_name: Glycol

smiles: OCCO

substrate: Glycol


substrate_name: Glycerol

smiles: C(C(CO)O)O

substrate: Glycerol


substrate_name: L-threitol

smiles: C(C(C(CO)O)O)O

substrate: L-threitol


substrate_name: D-threitol

substrate: D-threitol


substrate_name: L-arabitol

substrate: L-arabitol


substrate_name: D-arabitol

substrate: D-arabitol


substrate_name: meso-galactitol

smiles: C(C(C(C(C(CO)O)O)O)O)O

substrate: meso-galactitol


substrate_name: L-glucitol

substrate: L-glucitol


substrate_name: L-mannitol

substrate: L-mannitol


substrate_name: D-mannitol

substrate: D-mannitol


substrate_name: L-altritol

smiles: OCC(O)C(O)C(O)C(O)CO

substrate: L-altritol


substrate_name: D-altritol

substrate: D-altritol


substrate_name: L-iditol

substrate: L-iditol


substrate_name: D-iditol

substrate: D-iditol


substrate_name: D-glycero-L-allo-heptitol

substrate: D-glycero-L-allo-heptitol


substrate_name: D-glycero-L-gulo-heptitol

smiles: C(C(C(C(C(C(CO)O)O)O)O)O)O

substrate: D-glycero-L-gulo-heptitol


smiles: CC(CO)O


smiles: C=CC(O)CO


organism: Rattus norvegicus

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NAD+

column_label: Table 1, Oxidation section, substrate row 'S-Indan-1-ol'; TBER1 column 'Km (uM)' = 134; TBER1 column 'kcat (min^-1)' = 25; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.19.

normalized_value_unit_evidence: Km: printed cell 134 uM; 134 / 1000 = 0.134 mM; the exported value is 0.134 mM. kcat: printed cell 25 min^-1; the printed turnover column is per MINUTE, so 25 / 60 = 0.4166666666666667 s^(-1); the exported value is 0.4166666667 s^(-1). kcat/Km: printed cell 0.19 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.19 x 1000 / 60 = 3.1666666666666665 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Oxidation row 'S-Indan-1-ol', TBER1 column group: Km = 134 uM; kcat = 25 min^-1; printed kcat/Km = 0.19 min^-1 uM^-1.

row_label: Table 1, Oxidation section, substrate row 'S-Indan-1-ol', TBER1 column group

table_label: Table 1: Substrate specificity for non-steroidal substrates in the oxidation and reduction reactions catalyzed by TBER1 and rat 17HSD5 (J. Biochem. 2006, 139(6):1053-1063, journal page 1058)


substrate_name: S-Camphorquinone

column_label: Table 1, Reduction section, substrate row 'S-Camphorquinone'; TBER1 column 'Km (uM)' = 9.9; TBER1 column 'kcat (min^-1)' = 113; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 11.

normalized_value_unit_evidence: Km: printed cell 9.9 uM; 9.9 / 1000 = 0.0099 mM; the exported value is 0.0099 mM. kcat: printed cell 113 min^-1; the printed turnover column is per MINUTE, so 113 / 60 = 1.8833333333333333 s^(-1); the exported value is 1.8833333333 s^(-1). kcat/Km: printed cell 11 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 11 x 1000 / 60 = 183.33333333333334 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row 'S-Camphorquinone', TBER1 column group: Km = 9.9 uM; kcat = 113 min^-1; printed kcat/Km = 11 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row 'S-Camphorquinone', TBER1 column group

substrate: S-Camphorquinone


enzyme_display_name: 17beta-estradiol 17-dehydrogenase

column_label: Table 1, Reduction section, substrate row 'S-Camphorquinone'; Rat 17HSD5 column 'Km (uM)' = 5.7; Rat 17HSD5 column 'kcat (min^-1)' = 12; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 2.1.

normalized_value_unit_evidence: Km: printed cell 5.7 uM; 5.7 / 1000 = 0.0057 mM; the exported value is 0.0057 mM. kcat: printed cell 12 min^-1; the printed turnover column is per MINUTE, so 12 / 60 = 0.2 s^(-1); the exported value is 0.2 s^(-1). kcat/Km: printed cell 2.1 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 2.1 x 1000 / 60 = 35.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row 'S-Camphorquinone', Rat 17HSD5 column group: Km = 5.7 uM; kcat = 12 min^-1; printed kcat/Km = 2.1 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row 'S-Camphorquinone', Rat 17HSD5 column group

enzyme: 17beta-estradiol 17-dehydrogenase


substrate_name: R-Camphorquinone

column_label: Table 1, Reduction section, substrate row 'R-Camphorquinone'; TBER1 column 'Km (uM)' = 33; TBER1 column 'kcat (min^-1)' = 78; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 2.4.

normalized_value_unit_evidence: Km: printed cell 33 uM; 33 / 1000 = 0.033 mM; the exported value is 0.033 mM. kcat: printed cell 78 min^-1; the printed turnover column is per MINUTE, so 78 / 60 = 1.3 s^(-1); the exported value is 1.3 s^(-1). kcat/Km: printed cell 2.4 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 2.4 x 1000 / 60 = 40.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row 'R-Camphorquinone', TBER1 column group: Km = 33 uM; kcat = 78 min^-1; printed kcat/Km = 2.4 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row 'R-Camphorquinone', TBER1 column group

substrate: R-Camphorquinone


column_label: Table 1, Reduction section, substrate row 'R-Camphorquinone'; Rat 17HSD5 column 'Km (uM)' = 1.6; Rat 17HSD5 column 'kcat (min^-1)' = 11; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 6.9.

normalized_value_unit_evidence: Km: printed cell 1.6 uM; 1.6 / 1000 = 0.0016 mM; the exported value is 0.0016 mM. kcat: printed cell 11 min^-1; the printed turnover column is per MINUTE, so 11 / 60 = 0.18333333333333332 s^(-1); the exported value is 0.1833333333 s^(-1). kcat/Km: printed cell 6.9 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 6.9 x 1000 / 60 = 115.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row 'R-Camphorquinone', Rat 17HSD5 column group: Km = 1.6 uM; kcat = 11 min^-1; printed kcat/Km = 6.9 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row 'R-Camphorquinone', Rat 17HSD5 column group


assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADP+

column_label: Table 2, (A) section, substrate row '5b-Androstan-17b-ol-3-one'; Rat 17HSD5 column 'Km (uM)' = 31; Rat 17HSD5 column 'kcat (min^-1)' = 22; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.71.

normalized_value_unit_evidence: Km: printed cell 31 uM; 31 / 1000 = 0.031 mM; the exported value is 0.031 mM. kcat: printed cell 22 min^-1; the printed turnover column is per MINUTE, so 22 / 60 = 0.36666666666666664 s^(-1); the exported value is 0.3666666667 s^(-1). kcat/Km: printed cell 0.71 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.71 x 1000 / 60 = 11.833333333333334 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row '5b-Androstan-17b-ol-3-one', Rat 17HSD5 column group: Km = 31 uM; kcat = 22 min^-1; printed kcat/Km = 0.71 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row '5b-Androstan-17b-ol-3-one', Rat 17HSD5 column group

table_label: Table 2: Substrate specificity for steroids in the oxidation and reduction reactions catalyzed by TBER1 and rat 17HSD5 (J. Biochem. 2006, 139(6):1053-1063, journal page 1058)


column_label: Table 2, (B) section, substrate row '4-Pregenene-17a,20a-diol-3-one'; TBER1 column 'Km (uM)' = 12; TBER1 column 'kcat (min^-1)' = 7.9; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.66.

normalized_value_unit_evidence: Km: printed cell 12 uM; 12 / 1000 = 0.012 mM; the exported value is 0.012 mM. kcat: printed cell 7.9 min^-1; the printed turnover column is per MINUTE, so 7.9 / 60 = 0.13166666666666668 s^(-1); the exported value is 0.1316666667 s^(-1). kcat/Km: printed cell 0.66 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.66 x 1000 / 60 = 11.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (B) row '4-Pregenene-17a,20a-diol-3-one', TBER1 column group: Km = 12 uM; kcat = 7.9 min^-1; printed kcat/Km = 0.66 min^-1 uM^-1.

row_label: Table 2, (B) section, substrate row '4-Pregenene-17a,20a-diol-3-one', TBER1 column group


column_label: Table 2, (B) section, substrate row '4-Pregenen-20a-ol-3-one'; TBER1 column 'Km (uM)' = 8.1; TBER1 column 'kcat (min^-1)' = 3.2; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.39.

normalized_value_unit_evidence: Km: printed cell 8.1 uM; 8.1 / 1000 = 0.0081 mM; the exported value is 0.0081 mM. kcat: printed cell 3.2 min^-1; the printed turnover column is per MINUTE, so 3.2 / 60 = 0.05333333333333334 s^(-1); the exported value is 0.0533333333 s^(-1). kcat/Km: printed cell 0.39 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.39 x 1000 / 60 = 6.5 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (B) row '4-Pregenen-20a-ol-3-one', TBER1 column group: Km = 8.1 uM; kcat = 3.2 min^-1; printed kcat/Km = 0.39 min^-1 uM^-1.

row_label: Table 2, (B) section, substrate row '4-Pregenen-20a-ol-3-one', TBER1 column group


column_label: Table 2, (B) section, substrate row '4-Pregenen-20a-ol-3-one'; Rat 17HSD5 column 'Km (uM)' = 24; Rat 17HSD5 column 'kcat (min^-1)' = 8.5; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.35.

normalized_value_unit_evidence: Km: printed cell 24 uM; 24 / 1000 = 0.024 mM; the exported value is 0.024 mM. kcat: printed cell 8.5 min^-1; the printed turnover column is per MINUTE, so 8.5 / 60 = 0.14166666666666666 s^(-1); the exported value is 0.1416666667 s^(-1). kcat/Km: printed cell 0.35 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.35 x 1000 / 60 = 5.833333333333333 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (B) row '4-Pregenen-20a-ol-3-one', Rat 17HSD5 column group: Km = 24 uM; kcat = 8.5 min^-1; printed kcat/Km = 0.35 min^-1 uM^-1.

row_label: Table 2, (B) section, substrate row '4-Pregenen-20a-ol-3-one', Rat 17HSD5 column group


column_label: Table 2, (B) section, substrate row '4-Androsten-3a-ol-17-one'; TBER1 column 'Km (uM)' = 14; TBER1 column 'kcat (min^-1)' = 3.6; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.25.

normalized_value_unit_evidence: Km: printed cell 14 uM; 14 / 1000 = 0.014 mM; the exported value is 0.014 mM. kcat: printed cell 3.6 min^-1; the printed turnover column is per MINUTE, so 3.6 / 60 = 0.060000000000000005 s^(-1); the exported value is 0.06 s^(-1). kcat/Km: printed cell 0.25 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.25 x 1000 / 60 = 4.166666666666667 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (B) row '4-Androsten-3a-ol-17-one', TBER1 column group: Km = 14 uM; kcat = 3.6 min^-1; printed kcat/Km = 0.25 min^-1 uM^-1.

row_label: Table 2, (B) section, substrate row '4-Androsten-3a-ol-17-one', TBER1 column group


column_label: Table 2, (C) section, substrate row '4-Androsten-3a-ol-17-one'; TBER1 column 'Km (uM)' = 13; TBER1 column 'kcat (min^-1)' = 1.3; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.10.

normalized_value_unit_evidence: Km: printed cell 13 uM; 13 / 1000 = 0.013 mM; the exported value is 0.013 mM. kcat: printed cell 1.3 min^-1; the printed turnover column is per MINUTE, so 1.3 / 60 = 0.021666666666666667 s^(-1); the exported value is 0.0216666667 s^(-1). kcat/Km: printed cell 0.10 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.10 x 1000 / 60 = 1.6666666666666667 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (C) row '4-Androsten-3a-ol-17-one', TBER1 column group: Km = 13 uM; kcat = 1.3 min^-1; printed kcat/Km = 0.10 min^-1 uM^-1.

row_label: Table 2, (C) section, substrate row '4-Androsten-3a-ol-17-one', TBER1 column group


column_label: Table 2, (C) section, substrate row '4-Androsten-3a-ol-17-one'; Rat 17HSD5 column 'Km (uM)' = 8.5; Rat 17HSD5 column 'kcat (min^-1)' = 1.9; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.22.

normalized_value_unit_evidence: Km: printed cell 8.5 uM; 8.5 / 1000 = 0.0085 mM; the exported value is 0.0085 mM. kcat: printed cell 1.9 min^-1; the printed turnover column is per MINUTE, so 1.9 / 60 = 0.03166666666666666 s^(-1); the exported value is 0.0316666667 s^(-1). kcat/Km: printed cell 0.22 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.22 x 1000 / 60 = 3.6666666666666665 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (C) row '4-Androsten-3a-ol-17-one', Rat 17HSD5 column group: Km = 8.5 uM; kcat = 1.9 min^-1; printed kcat/Km = 0.22 min^-1 uM^-1.

row_label: Table 2, (C) section, substrate row '4-Androsten-3a-ol-17-one', Rat 17HSD5 column group


column_label: Table 2, (D) section, substrate row '5b-Pregnan-20a-ol-3-one'; TBER1 column 'Km (uM)' = 19; TBER1 column 'kcat (min^-1)' = 1.0; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.05.

normalized_value_unit_evidence: Km: printed cell 19 uM; 19 / 1000 = 0.019 mM; the exported value is 0.019 mM. kcat: printed cell 1.0 min^-1; the printed turnover column is per MINUTE, so 1.0 / 60 = 0.016666666666666666 s^(-1); the exported value is 0.0166666667 s^(-1). kcat/Km: printed cell 0.05 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.05 x 1000 / 60 = 0.8333333333333334 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (D) row '5b-Pregnan-20a-ol-3-one', TBER1 column group: Km = 19 uM; kcat = 1.0 min^-1; printed kcat/Km = 0.05 min^-1 uM^-1.

row_label: Table 2, (D) section, substrate row '5b-Pregnan-20a-ol-3-one', TBER1 column group


enzyme_display_name: 4-hydroxyproline epimerase

organism: Brucella melitensis biotype 1

enzyme: 4-hydroxyproline epimerase


organism: Aeromonas hydrophila

substrate_name: Carbenicillin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Carbenicillin' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 450 +/- 30; column 'kcat (s-1)' = 16.00; column 'kcat/Km (M-1 s-1)' = 3.60 x 10^4.

normalized_value_unit_evidence: Km: printed Table 3 cell 450 uM; 450 / 1000 = 0.45 mM; the exported value is 0.45 mM. kcat: printed Table 3 cell 16.00 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 16 s^(-1). kcat/Km: printed Table 3 cell 3.60 x 10^4 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 36000 / 1000 = 36 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Carbenicillin': Km = 450 +/- 30 uM; kcat = 16.00 s^-1; printed kcat/Km = 3.60 x 10^4 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Carbenicillin' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Carbenicillin


substrate_name: Penicillin G

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Penicillin G' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 700 +/- 50; column 'kcat (s-1)' = 5.21; column 'kcat/Km (M-1 s-1)' = 7.40 x 10^3.

normalized_value_unit_evidence: Km: printed Table 3 cell 700 uM; 700 / 1000 = 0.7 mM; the exported value is 0.7 mM. kcat: printed Table 3 cell 5.21 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 5.21 s^(-1). kcat/Km: printed Table 3 cell 7.40 x 10^3 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 7400 / 1000 = 7.4 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Penicillin G': Km = 700 +/- 50 uM; kcat = 5.21 s^-1; printed kcat/Km = 7.40 x 10^3 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Penicillin G' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Penicillin G


substrate_name: Oxacillin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Oxacillin' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 25 +/- 3; column 'kcat (s-1)' = 0.75; column 'kcat/Km (M-1 s-1)' = 3.00 x 10^4.

normalized_value_unit_evidence: Km: printed Table 3 cell 25 uM; 25 / 1000 = 0.025 mM; the exported value is 0.025 mM. kcat: printed Table 3 cell 0.75 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.75 s^(-1). kcat/Km: printed Table 3 cell 3.00 x 10^4 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 30000 / 1000 = 30 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Oxacillin': Km = 25 +/- 3 uM; kcat = 0.75 s^-1; printed kcat/Km = 3.00 x 10^4 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Oxacillin' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Oxacillin


substrate_name: Cephaloridin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cephaloridin' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 190 +/- 8; column 'kcat (s-1)' = 0.12; column 'kcat/Km (M-1 s-1)' = 6.30 x 10^2.

normalized_value_unit_evidence: Km: printed Table 3 cell 190 uM; 190 / 1000 = 0.19 mM; the exported value is 0.19 mM. kcat: printed Table 3 cell 0.12 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.12 s^(-1). kcat/Km: printed Table 3 cell 6.30 x 10^2 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 630 / 1000 = 0.63 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Cephaloridin': Km = 190 +/- 8 uM; kcat = 0.12 s^-1; printed kcat/Km = 6.30 x 10^2 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cephaloridin' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Cephaloridin


substrate_name: Cephaloglycin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cephaloglycin' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 280 +/- 30; column 'kcat (s-1)' = 0.20; column 'kcat/Km (M-1 s-1)' = 7.10 x 10^2.

normalized_value_unit_evidence: Km: printed Table 3 cell 280 uM; 280 / 1000 = 0.28 mM; the exported value is 0.28 mM. kcat: printed Table 3 cell 0.20 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.2 s^(-1). kcat/Km: printed Table 3 cell 7.10 x 10^2 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 710 / 1000 = 0.71 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Cephaloglycin': Km = 280 +/- 30 uM; kcat = 0.20 s^-1; printed kcat/Km = 7.10 x 10^2 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cephaloglycin' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Cephaloglycin


substrate_name: Cefuroxime

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cefuroxime' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 20 +/- 3; column 'kcat (s-1)' = 0.07; column 'kcat/Km (M-1 s-1)' = 3.50 x 10^3.

normalized_value_unit_evidence: Km: printed Table 3 cell 20 uM; 20 / 1000 = 0.02 mM; the exported value is 0.02 mM. kcat: printed Table 3 cell 0.07 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.07 s^(-1). kcat/Km: printed Table 3 cell 3.50 x 10^3 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 3500 / 1000 = 3.5 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Cefuroxime': Km = 20 +/- 3 uM; kcat = 0.07 s^-1; printed kcat/Km = 3.50 x 10^3 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cefuroxime' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Cefuroxime


substrate_name: Cefotaxime

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cefotaxime' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 26 +/- 3; column 'kcat (s-1)' = 0.07; column 'kcat/Km (M-1 s-1)' = 2.70 x 10^3.

normalized_value_unit_evidence: Km: printed Table 3 cell 26 uM; 26 / 1000 = 0.026 mM; the exported value is 0.026 mM. kcat: printed Table 3 cell 0.07 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.07 s^(-1). kcat/Km: printed Table 3 cell 2.70 x 10^3 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 2700 / 1000 = 2.7 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Cefotaxime': Km = 26 +/- 3 uM; kcat = 0.07 s^-1; printed kcat/Km = 2.70 x 10^3 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cefotaxime' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Cefotaxime


substrate_name: Imipenem

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Imipenem' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 80 +/- 8; column 'kcat (s-1)' = 168.00; column 'kcat/Km (M-1 s-1)' = 2.10 x 10^6.

normalized_value_unit_evidence: Km: printed Table 3 cell 80 uM; 80 / 1000 = 0.08 mM; the exported value is 0.08 mM. kcat: printed Table 3 cell 168.00 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 168 s^(-1). kcat/Km: printed Table 3 cell 2.10 x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 2100000 / 1000 = 2100 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Imipenem': Km = 80 +/- 8 uM; kcat = 168.00 s^-1; printed kcat/Km = 2.10 x 10^6 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Imipenem' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Imipenem


column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row '6-beta-IP' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 530 +/- 50; column 'kcat (s-1)' = 0.64; column 'kcat/Km (M-1 s-1)' = 1.20 x 10^3.

normalized_value_unit_evidence: Km: printed Table 3 cell 530 uM; 530 / 1000 = 0.53 mM; the exported value is 0.53 mM. kcat: printed Table 3 cell 0.64 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.64 s^(-1). kcat/Km: printed Table 3 cell 1.20 x 10^3 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1200 / 1000 = 1.2 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row '6-beta-IP': Km = 530 +/- 50 uM; kcat = 0.64 s^-1; printed kcat/Km = 1.20 x 10^3 M^-1 s^-1.

row_label: Table 3, antibiotic row '6-beta-IP' within the 'Aeromonas hydrophila A2 enzyme' column group


substrate_name: Sulbactam

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Sulbactam' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 37 +/- 4; column 'kcat (s-1)' = 0.12; column 'kcat/Km (M-1 s-1)' = 3.24 x 10^3.

normalized_value_unit_evidence: Km: printed Table 3 cell 37 uM; 37 / 1000 = 0.037 mM; the exported value is 0.037 mM. kcat: printed Table 3 cell 0.12 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.12 s^(-1). kcat/Km: printed Table 3 cell 3.24 x 10^3 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 3240 / 1000 = 3.24 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Sulbactam': Km = 37 +/- 4 uM; kcat = 0.12 s^-1; printed kcat/Km = 3.24 x 10^3 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Sulbactam' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Sulbactam


organism: Stenotrophomonas maltophilia

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row '6-beta-IP' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 640 +/- 30; column 'kcat (s-1)' = 648; column 'kcat/Km (M-1 s-1)' = 1.00 x 10^6.

normalized_value_unit_evidence: Km: printed Table 3 cell 640 uM; 640 / 1000 = 0.64 mM; the exported value is 0.64 mM. kcat: printed Table 3 cell 648 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 648 s^(-1). kcat/Km: printed Table 3 cell 1.00 x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1000000 / 1000 = 1000 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row '6-beta-IP': Km = 640 +/- 30 uM; kcat = 648 s^-1; printed kcat/Km = 1.00 x 10^6 M^-1 s^-1.

row_label: Table 3, antibiotic row '6-beta-IP' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group


column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row '6-beta-IP' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 7460 +/- 590; column 'kcat (s-1)' = 450; column 'kcat/Km (M-1 s-1)' = 6.00 x 10^4.

normalized_value_unit_evidence: Km: printed Table 3 cell 7460 uM; 7460 / 1000 = 7.46 mM; the exported value is 7.46 mM. kcat: printed Table 3 cell 450 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 450 s^(-1). kcat/Km: printed Table 3 cell 6.00 x 10^4 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 60000 / 1000 = 60 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row '6-beta-IP': Km = 7460 +/- 590 uM; kcat = 450 s^-1; printed kcat/Km = 6.00 x 10^4 M^-1 s^-1.

row_label: Table 3, antibiotic row '6-beta-IP' within the 'Bacillus cereus beta-lactamase II' column group


enzyme_display_name: alcohol dehydrogenase

organism: Thermococcus kodakarensis

enzyme: alcohol dehydrogenase


enzyme_display_name: cyanidin 3-O-glucoside 5-O-glucosyltransferase (acyl-glucose)

organism: Dianthus caryophyllus

assay_conditions_summary: pH 6; 303.15 K; buffer 0.1 M citrate

column_label: Results prose page 3380, recombinant Dc AA5GT clause of the kinetic parameter sentence: 'kcat for Cy3G = 0.07 s^-1' and 'Km for Cy3G = 6.5 uM'; Results prose page 3381, recombinant Dc AA5GT sentence: '5-O-glucosyltransferase activity for Cy3G (kcat/Km = 1.0 x 10^4)', in the s^-1 M^-1 unit the article states for kcat/Km on page 3380.

normalized_value_unit_evidence: kcat: printed value 0.07 s^-1; already in s^-1, no unit conversion applies; the paper value is 0.07 s^(-1). Km: printed value 6.5 uM; 6.5 / 1000 = 0.0065 mM. kcat/Km: printed value 1.0 x 10^4 s^-1 M^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 10000 / 1000 = 10.0 s^-1 mM^-1; the export carries no kcat_over_km field for this row, so the printed value is recorded as evidence only and no ratio is written.

raw_value_unit_evidence: Results prose, page 3380, recombinant Dc AA5GT clause: kcat for Cy3G = 0.07 s^-1; Km for Cy3G = 6.5 uM. Results prose, page 3381, recombinant Dc AA5GT sentence: printed kcat/Km for Cy3G = 1.0 x 10^4 s^-1 M^-1.

row_label: page-3380 kinetic parameter sentence, recombinant Dc AA5GT clause, Cy3G acceptor values; page-3381 recombinant Dc AA5GT efficiency sentence, Cy3G value

table_label: Results prose, pages 3380 and 3381, section 'Kinetic Properties and Substrate Preferences of Acceptor and Donor Molecules of Dc AA5GT and Dg AA7GT' (The Plant Cell 2010, 22:3374-3389); the article has no kinetic table, the sentences cite the online-only Supplemental Table 3


substrate_name: Cephalothin

column_label: Table 4 (Substrate profile of C. diversus beta-lactamases), 'Form II' group, substrate row 'Cephalothin'; column 'Km (uM)' = 41 +/- 2; column 'kcat (s-1)' = 55 +/- 6; column 'kcat/Km (M-1 s-1)' = (1.30 +/- 0.20) x 10^6.

normalized_value_unit_evidence: Km: printed Table 4 cell 41 +/- 2 uM; 41 / 1000 = 0.041 mM; the exported value is 0.041 mM. kcat: printed Table 4 cell 55 +/- 6 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 55 s^(-1). kcat/Km: printed Table 4 cell (1.30 +/- 0.20) x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1300000 / 1000 = 1300 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 4, 'Form II' group, substrate row 'Cephalothin': Km = 41 +/- 2 uM; kcat = 55 +/- 6 s^-1; printed kcat/Km = (1.30 +/- 0.20) x 10^6 M^-1 s^-1.

substrate: Cephalothin


substrate_name: Cephalosporin C

column_label: Table 4 (Substrate profile of C. diversus beta-lactamases), 'Form II' group, substrate row 'Cephalosporin C'; column 'Km (uM)' = 123 +/- 13; column 'kcat (s-1)' = 23 +/- 9; column 'kcat/Km (M-1 s-1)' = (0.19 +/- 0.09) x 10^6.

normalized_value_unit_evidence: Km: printed Table 4 cell 123 +/- 13 uM; 123 / 1000 = 0.123 mM; the exported value is 0.123 mM. kcat: printed Table 4 cell 23 +/- 9 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 23 s^(-1). kcat/Km: printed Table 4 cell (0.19 +/- 0.09) x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 190000 / 1000 = 190 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 4, 'Form II' group, substrate row 'Cephalosporin C': Km = 123 +/- 13 uM; kcat = 23 +/- 9 s^-1; printed kcat/Km = (0.19 +/- 0.09) x 10^6 M^-1 s^-1.

substrate: Cephalosporin C


substrate_name: acetaldehyde

substrate: acetaldehyde


substrate_name: propanal

substrate: propanal


substrate_name: pentanal

substrate: pentanal


substrate_name: hexanal

substrate: hexanal


substrate_name: heptanal

substrate: heptanal


substrate_name: decanal

substrate: decanal


substrate_name: o-nitro-

substrate: o-nitro-


substrate_name: p-nitro-

substrate: p-nitro-


substrate_name: p-methyl-

substrate: p-methyl-


substrate_name: m-methyl-

substrate: m-methyl-


substrate_name: p-(dimethylamino)-

substrate: p-(dimethylamino)-


substrate_name: m-methoxy-

substrate: m-methoxy-


substrate_name: o-methoxy-

substrate: o-methoxy-


substrate_name: o-methyl-

substrate: o-methyl-


substrate_name: o-hydroxy-

substrate: o-hydroxy-


substrate_name: (meso)-erythritol

substrate: (meso)-erythritol


substrate_name: (meso)-ribitol

substrate: (meso)-ribitol


substrate_name: (meso)-xylitol

substrate: (meso)-xylitol


substrate_name: D-glucitol (sorbitol)

substrate: D-glucitol (sorbitol)


substrate_name: D-glycero-D-manno-heptitol (volemitol)

substrate: D-glycero-D-manno-heptitol (volemitol)


substrate_name: 1,2,3-hexanetriol (mixture of D-, L- and meso forms)

smiles: CCCC(C(CO)O)O


enzyme_display_name: ferredoxin--nitrite reductase

organism: Nicotiana tabacum

substrate_name: nitrite

source_db: skid

source_license: CC BY-NC-ND 4.0

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

enzyme: ferredoxin--nitrite reductase

substrate: nitrite

source: skid


sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


organism: Oryza sativa

substrate_name: methyl viologen

source_db: brenda

kcat_display: Not reported

source_license: CC BY 4.0

substrate: methyl viologen

source: brenda


enzyme_display_name: nitroalkane oxidase

organism: Streptomyces ansochromogenes

source_license: CC BY 4.0; CC BY-NC-ND 4.0

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

proof_lines:
pH 8.0, 28°C
Raw SKiD Env: Temp=28°C, pH=8

source_databases_merged: brenda skid

enzyme: nitroalkane oxidase


enzyme_display_name: ferredoxin--nitrate reductase

organism: Haloferax mediterranei

enzyme: ferredoxin--nitrate reductase


organism: Pyropia yezoensis


enzyme_display_name: hydroxylamine reductase

organism: Pseudomonas aeruginosa

enzyme: hydroxylamine reductase


enzyme_display_name: trypanothione-disulfide reductase

organism: Leishmania donovani

substrate_name: trypanothione disulfide

assay_conditions_summary: cofactor NADPH

enzyme: trypanothione-disulfide reductase

substrate: trypanothione disulfide


organism: Rhodobacter capsulatus

substrate_name: methyl viologen (at pH 9.3 and 40 degrees Celsius)

source_db: uniprot

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

source: uniprot


organism: Trypanosoma brucei


organism: Euglena gracilis


organism: Trypanosoma congolense


substrate_name: trypanothione

sequence_source: Trusted canonical UniProt P13110 sequence evidence, reusable per the supplied identity-reuse summary.

data_origin: experimental

substrate: trypanothione


enzyme_display_name: CoA-disulfide reductase

organism: Pyrococcus horikoshii

substrate_name: H+; NADH; CoA-disulfide

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

sequence_source: UniProtKB:O58308 direct accession lookup

enzyme: CoA-disulfide reductase

substrate: H+; NADH; CoA-disulfide


organism: Staphylococcus aureus (strain NCTC 8325 / PS 47)

substrate_name: NADPH

substrate: NADPH


organism: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)


enzyme_display_name: glutathione amide reductase

organism: Marichromatium gracile

substrate_name: glutathione amide disulfide (GASSAG) for the reverse reaction (in the presence of 100 uM NADH)

enzyme: glutathione amide reductase


verification_status: corrected

km_display: Not reported

sequence_source: UniProtKB/Swiss-Prot O58308; PubMed 15720393 identity/function anchor

assay_conditions_summary: 348.15 K; cofactor NADPH

status: corrected


assay_conditions_summary: pH 7.1; 298.15 K; cofactor NADPH


enzyme_display_name: NAD(P)H sulfur oxidoreductase (CoA-dependent)


organism: Rhodococcus erythropolis

smiles: C(CC(=O)[O-])CSSCCCC(=O)[O-]

assay_conditions_summary: pH 8; 310.15 K; cofactor NADH


organism: Xanthobacter autotrophicus

source_db: oed

smiles: CC(=O)CSCCS(=O)(=O)O

mutation_type: substitution

source: oed


organism: Xanthobacter sp.

substrate_name: acetoacetate

substrate: acetoacetate


enzyme_display_name: glutathione-disulfide reductase

organism: Caenorhabditis elegans

substrate_name: glutathione disulfide (at 25 degrees Celsius)

enzyme: glutathione-disulfide reductase


organism: Acidithiobacillus ferrooxidans

substrate_name: GSSG

substrate: GSSG


organism: Enterococcus faecalis

substrate_name: H+; NADPH; Glutathione disulfide

substrate: H+; NADPH; Glutathione disulfide


organism: Escherichia coli

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADPH


enzyme_display_name: cystine reductase

organism: Homo sapiens

substrate_name: L-cystine

enzyme: cystine reductase

substrate: L-cystine


substrate_name: NADP+

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADP+

substrate: NADP+


organism: Haemonchus contortus

substrate_name: glutathione disulfide

assay_conditions_summary: pH 7.8; 310.15 K; cofactor FAD

substrate: glutathione disulfide


substrate_name: reduced glutathione

substrate: reduced glutathione


assay_conditions_summary: pH 7.8; 310.15 K; cofactor NADPH


organism: Mus musculus

substrate_name: Oxidized glutathione

substrate: Oxidized glutathione


organism: Escherichia coli (strain K12)


organism: Euglena gracilis


substrate_name: NADPH; Glutathione disulfide; H+

substrate: NADPH; Glutathione disulfide; H+


organism: Oryza sativa


organism: Phycomyces blakesleeanus


organism: Neospora caninum

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NADPH


organism: Trypanosoma congolense


enzyme_display_name: protein-disulfide reductase

organism: Methanosarcina acetivorans

substrate_name: coenzyme M

assay_conditions_summary: pH 6.8; 323.15 K; cofactor CoA

enzyme: protein-disulfide reductase

substrate: coenzyme M


enzyme_display_name: thioredoxin-disulfide reductase (NADPH)

organism: Aeropyrum pernix

substrate_name: 5,5'-dithiobis(2-nitrobenzoic acid)

enzyme: thioredoxin-disulfide reductase (NADPH)

substrate: 5,5'-dithiobis(2-nitrobenzoic acid)


organism: Sus scrofa


organism: Leptospira interrogans serovar Copenhageni

assay_conditions_summary: pH 7; 303.15 K; cofactor NADPH


substrate_name: thioredoxin disulfide

substrate: thioredoxin disulfide


organism: Rattus norvegicus

assay_conditions_summary: pH 7.6; 303.15 K; cofactor NADPH


organism: Arabidopsis thaliana

substrate_name: thioredoxin

substrate: thioredoxin


organism: Deinococcus radiophilus

assay_conditions_summary: pH 7; 295.15 K; buffer context (50 mM potassium phosphate; cofactor NADPH

proof_lines: in 50 mM potassium phosphate buffer (pH 7.0), at 22°C


organism: Plasmodium falciparum (isolate FCH-5)

substrate_name: NADPH (at 20 degrees Celsius and pH 7.4)

assay_conditions_summary: pH 7.4; cofactor NADPH


organism: Taenia crassiceps


enzyme_display_name: sulfite dehydrogenase (cytochrome)

organism: Starkeya novella

substrate_name: sulfite

enzyme: sulfite dehydrogenase (cytochrome)

substrate: sulfite


organism: Thermotoga maritima

substrate_name: benzyl viologen

assay_conditions_summary: pH 9.5; 353.15 K; cofactor NADPH

proof_lines:
at pH 9.5 and 80°C
at pH 6.5 and 80°C

substrate: benzyl viologen


enzyme_display_name: thiosulfate dehydrogenase

organism: Pseudomonas sp.

substrate_name: thiosulfate

enzyme: thiosulfate dehydrogenase

substrate: thiosulfate


enzyme_display_name: thiol oxidase

organism: Aspergillus oryzae

smiles: OCCS

enzyme: thiol oxidase


enzyme_display_name: sulfide-cytochrome-c reductase (flavocytochrome c)

organism: Allochromatium vinosum

substrate_name: Sulfide

assay_conditions_summary: pH 8.5; 293.15 K; buffer 10 mM Tris-HCl

proof_lines: in 10 mM Tris-HCl buffer, pH 8.5, at 20°C

substrate: Sulfide


substrate_name: N-acetylcysteine

substrate: N-acetylcysteine


substrate_name: D-Cys

substrate: D-Cys


enzyme_display_name: methanethiol oxidase

organism: Hyphomicrobium sp.

enzyme: methanethiol oxidase


enzyme_display_name: prenylcysteine oxidase

enzyme: prenylcysteine oxidase


enzyme_display_name: formylglycine-generating enzyme

organism: Thermomonospora curvata

enzyme: formylglycine-generating enzyme


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

substrate_name: 5'-adenylyl sulfate (at pH 8.5 and 30 degrees Celsius)


enzyme_display_name: glutathione oxidase

enzyme: glutathione oxidase


enzyme_display_name: L-methionine (S)-S-oxide reductase

organism: Escherichia coli B

substrate_name: L-methionine (S)-S-oxide

enzyme: L-methionine (S)-S-oxide reductase

substrate: L-methionine (S)-S-oxide


enzyme_display_name: L-methionine (R)-S-oxide reductase

organism: Escherichia coli

substrate_name: L-Methionine (R)-S-oxide; Reduced thioredoxin

kcat_over_km_origin: calculated

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

source_databases_merged: sabio_rk brenda skid

enzyme: L-methionine (R)-S-oxide reductase


organism: Escherichia coli (strain K12)

substrate_name: L-methionine-(R)-S-oxide

substrate: L-methionine-(R)-S-oxide


organism: Saccharomyces cerevisiae

substrate_name: Reduced thioredoxin; L-Methionine (R)-S-oxide

sequence_source: UniProtKB REST direct lookup P36088; UniProtKB reviewed (Swiss-Prot)


organism: Mus musculus

substrate_name: H2O; O2; Methanethiol

substrate: H2O; O2; Methanethiol


organism: Thermoplasma acidophilum

substrate_name: L-methionine (R)-S-oxide

sequence_source: UniProt REST Q9HJW5 canonical sequence; not accepted as row-specific evidence for the unspecified variant

smiles: CS(=O)CCC(C(=O)O)N

substrate: L-methionine (R)-S-oxide


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: 3-HSA (with FAD at pH 7 and at 25 degrees Celsius)

assay_conditions_summary: cofactor FAD


enzyme_display_name: glutathione dehydrogenase (ascorbate)

organism: Homo sapiens

substrate_name: dehydroascorbate

sequence_source: UniProtKB REST accession Q9H4Y5 direct lookup on 2026-06-05

enzyme: glutathione dehydrogenase (ascorbate)

substrate: dehydroascorbate


enzyme_display_name: respiratory dimethylsulfoxide reductase

substrate_name: 2-mercaptopyridine N-oxide

enzyme: respiratory dimethylsulfoxide reductase

substrate: 2-mercaptopyridine N-oxide


substrate_name: 2-chloropyridine N-oxide (at pH 5 and at 30 degrees Celsius)


enzyme_display_name: glutathionyl-hydroquinone reductase

organism: Sphingobium chlorophenolicum

enzyme: glutathionyl-hydroquinone reductase


substrate_name: glutathione

smiles: C(CC(=O)NC(CS)C(=O)NCC(=O)O)C(C(=O)O)N

proof_lines:
pH 8.0, 23°C
Raw SKiD Env: Temp=23°C, pH=8
Raw SKiD Env: Temp=-----, pH=7.5

substrate: glutathione


enzyme_display_name: assimilatory sulfite reductase (ferredoxin)

organism: Limnospira platensis

substrate_name: S-Sulfoglutathione

substrate: S-Sulfoglutathione


enzyme_display_name: chlorate reductase

organism: Haloferax mediterranei

substrate_name: Chlorate

enzyme: chlorate reductase

substrate: Chlorate


enzyme_display_name: sulfiredoxin

substrate_name: ATP

assay_conditions_summary: pH 7; 303.15 K; buffer activity measured in TK; cofactor ATP

enzyme: sulfiredoxin

substrate: ATP


organism: Stutzerimonas chloritidismutans

sequence: LNMLEPVGETLASEYPYXK

wild_type_sequence: LNMLEPVGETLASEYPYXK


enzyme_display_name: nitrate reductase (cytochrome)

organism: Cereibacter sphaeroides f. sp. denitrificans

substrate_name: nitrate

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

enzyme: nitrate reductase (cytochrome)

substrate: nitrate


enzyme_display_name: homocysteine S-methyltransferase

organism: Arabidopsis thaliana

substrate_name: S-methylmethionine

enzyme: homocysteine S-methyltransferase

substrate: S-methylmethionine


substrate_name: L-homocysteine

substrate: L-homocysteine


organism: Brassica oleracea var. italica

substrate_name: DL-homocysteine

substrate: DL-homocysteine


enzyme_display_name: phosphoethanolamine N-methyltransferase

organism: Plasmodium vivax

substrate_name: Ethanolamine phosphate

sequence_source: UniProtKB:A5K867 direct lookup

smiles: C(COP(=O)(O)O)N

enzyme: phosphoethanolamine N-methyltransferase

substrate: Ethanolamine phosphate


enzyme_display_name: caffeoyl-CoA O-methyltransferase

organism: Petroselinum crispum

substrate_name: caffeoyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: caffeoyl-CoA O-methyltransferase

substrate: caffeoyl-CoA


organism: Pyrus x bretschneideri

assay_conditions_summary: pH 4; 310.15 K; cofactor CoA


organism: Triticum aestivum

substrate_name: S-Adenosyl-L-methionine; Caffeoyl-CoA

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

substrate: S-Adenosyl-L-methionine; Caffeoyl-CoA


organism: Vanilla planifolia

substrate_name: tricetin

substrate: tricetin


enzyme_display_name: tryptophan 2-C-methyltransferase

organism: Kitasatospora setae

substrate_name: L-tryptophan

enzyme: tryptophan 2-C-methyltransferase

substrate: L-tryptophan


enzyme_display_name: eukaryotic sulfide quinone oxidoreductase

substrate_name: sulfide (with sulfite as electron acceptor)


organism: Pseudomonas denitrificans


organism: Methanobacterium ivanovii

sequence_source: UniProtKB reviewed accession P29564


organism: Aspergillus parasiticus

substrate_name: dihydrodemethylsterigmatocystin

substrate: dihydrodemethylsterigmatocystin


organism: Pseudomonas denitrificans (nom. rej.)

substrate_name: uroporphyrinogen III

substrate: uroporphyrinogen III


enzyme_display_name: alcohol dehydrogenase

organism: Homo sapiens

substrate_name: all-trans-Retinal

enzyme: alcohol dehydrogenase

substrate: all-trans-Retinal


enzyme_display_name: sterigmatocystin 8-O-methyltransferase

substrate_name: sterigmatocystin

enzyme: sterigmatocystin 8-O-methyltransferase

substrate: sterigmatocystin


organism: Thalictrum flavum

substrate_name: (R,S)-Norreticuline

substrate: (R,S)-Norreticuline


substrate_name: S-Adenosyl-L-methionine

substrate: S-Adenosyl-L-methionine


enzyme_display_name: (S)-scoulerine 9-O-methyltransferase

organism: Coptis japonica

substrate_name: (S)-scoulerine

enzyme: (S)-scoulerine 9-O-methyltransferase

substrate: (S)-scoulerine


enzyme_display_name: methionine S-methyltransferase

organism: Wollastonia biflora

substrate_name: L-Met

smiles: CSCCC(C(=O)O)N

enzyme: methionine S-methyltransferase

substrate: L-Met


substrate_name: S-Adenosyl-L-methionine; (S)-Scoulerine

substrate: S-Adenosyl-L-methionine; (S)-Scoulerine


organism: Eschscholzia californica


enzyme_display_name: methionine synthase

organism: Escherichia coli

substrate_name: S-adenosyl-L-methionine

enzyme: methionine synthase

substrate: S-adenosyl-L-methionine


substrate_name: (S)-Stylopine

substrate: (S)-Stylopine


substrate_name: (R,S)-stylopine

substrate: (R,S)-stylopine


organism: Dehalococcoides mccartyi

substrate_name: L-homocysteine

smiles: C(CS)C(C(=O)O)N

substrate: L-homocysteine


organism: Rattus norvegicus

assay_conditions_summary: pH 7.4; 310.15 K; buffer 50 mM phosphate

proof_lines: in 50 mM phosphate buffer, pH 7.4, at 37°C


organism: Thermus thermophilus

sequence_source: UniProt REST Q5SKM5 canonical sequence


organism: Sus scrofa

ki_unit: mM


organism: Papaver bracteatum

substrate_name: (R,S)-Stylopine

substrate: (R,S)-Stylopine


organism: Papaver somniferum


enzyme_display_name: chlorophenol O-methyltransferase

organism: Trichoderma longibrachiatum

substrate_name: 2,4,6-trichlorophenol (2,4,6-TCP)

enzyme: chlorophenol O-methyltransferase

substrate: 2,4,6-trichlorophenol (2,4,6-TCP)


enzyme_display_name: arsenite methyltransferase

substrate_name: S-Adenosyl-L-methionine; Arsenite

enzyme: arsenite methyltransferase

substrate: S-Adenosyl-L-methionine; Arsenite


substrate_name: sodium arsenite

substrate: sodium arsenite


enzyme_display_name: (S)-coclaurine-N-methyltransferase

organism: Stephania intermedia

substrate_name: (R)-coclaurine

enzyme: (S)-coclaurine-N-methyltransferase

substrate: (R)-coclaurine


enzyme_display_name: columbamine O-methyltransferase

substrate_name: S-Adenosyl-L-methionine; Columbamine

enzyme: columbamine O-methyltransferase

substrate: S-Adenosyl-L-methionine; Columbamine


enzyme_display_name: jasmonate O-methyltransferase

organism: Arabidopsis thaliana

enzyme: jasmonate O-methyltransferase


substrate_name: (RS)-coclaurine

sequence_source: Selected-batch preserved sequence; compact direct UniProt REST check of Q948P7 confirmed it is length 358 and byte-identical to the live unreviewed canonical Q948P7_COPJA sequence.

proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=30°C, pH=8

substrate: (RS)-coclaurine


organism: Brassica rapa subsp. oleifera

substrate_name: jasmonate

substrate: jasmonate


substrate_name: (-)-Jasmonic acid

substrate: (-)-Jasmonic acid


organism: Thalictrum flavum subsp. glaucum

substrate_name: (R,S)-norreticuline

substrate: (R,S)-norreticuline


organism: Humulus lupulus

substrate_name: chalconaringenin

substrate: chalconaringenin


organism: Medicago sativa

substrate_name: isoliquiritigenin

substrate: isoliquiritigenin


organism: Aphanothece halophytica

substrate_name: S-adenosyl-L-homocysteine (at pH 8.8 and at 37 degrees Celsius)


enzyme_display_name: glycine/sarcosine N-methyltransferase

organism: Halorhodospira halochloris

substrate_name: sarcosine (at pH 7.4 and at 37 degrees Celsius)

smiles: CNCC(=O)O

enzyme: glycine/sarcosine N-methyltransferase


enzyme_display_name: theobromine synthase

organism: Coffea arabica

enzyme: theobromine synthase


organism: Camellia ptilophylla

substrate_name: 7-methylxanthine (in the presence of 50 uM S-adenosyl-L-methionine)


organism: Camellia irrawadiensis

substrate: 7-methylxanthine (in the presence of 50 uM S-adenosyl-L-methionine)


organism: Parasynechococcus marenigrum (strain WH8102)

substrate_name: AdoMet (with glycine at pH 8.2 and at 37 degrees Celsius)

assay_conditions_summary: pH 8


organism: Theobroma cacao


substrate_name: AdoMet (with dimethylglycine at pH 7.4 and at 37 degrees Celsius)


enzyme_display_name: caffeine synthase

organism: Camellia sinensis

enzyme: caffeine synthase


substrate_name: 7-methylxanthine for the recombinant protein


substrate_name: paraxanthine

substrate: paraxanthine


enzyme_display_name: tricin synthase

organism: Oryza sativa subsp. japonica

substrate_name: tricetin

enzyme: tricin synthase

substrate: tricetin


organism: Oryza sativa

substrate_name: S-Adenosyl-L-methionine; Tricetin

substrate: S-Adenosyl-L-methionine; Tricetin


organism: Zea mays


organism: Bacillus subtilis

substrate_name: S-adenosyl-L-methionine

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature N192A to reconstruct the assayed variant sequence.

substrate: S-adenosyl-L-methionine


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature K197A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature K133A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature N200A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature K204A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature K197A/N200A/E202A/K204A/K205A to reconstruct the assayed variant sequence.

proof_lines: pH 7.5, 25°C, mutant enzyme K197A/N200A/E202A/K204A/K205A


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature E202A to reconstruct the assayed variant sequence.


sequence_source: uniprot


enzyme_display_name: carnosine N-methyltransferase

organism: Gallus gallus

substrate_name: carnosine

enzyme: carnosine N-methyltransferase

substrate: carnosine


organism: Saccharomyces cerevisiae

substrate_name: S-Adenosyl-L-methionine; Carnosine

sequence_source: UniProtKB P53934 direct accession lookup; sequence matched the accession reuse cache.

substrate: S-Adenosyl-L-methionine; Carnosine


organism: Methanocaldococcus jannaschii

substrate_name: S-Adenosyl-L-methionine; tRNA guanine

proof_lines: SABIO-RK modifier species: tRNA (guanine37-N1)-methyltransferase(Enzyme) mutant Y176A His-tag

substrate: S-Adenosyl-L-methionine; tRNA guanine


proof_lines: SABIO-RK modifier species: tRNA (guanine37-N1)-methyltransferase(Enzyme) mutant R144A His-tag


organism: Streptomyces azureus


enzyme_display_name: mycinamicin VI 2''-O-methyltransferase

organism: Micromonospora griseorubida

substrate_name: mycinamicin VI

enzyme: mycinamicin VI 2''-O-methyltransferase

substrate: mycinamicin VI


enzyme_display_name: flavonoid 4'-O-methyltransferase

organism: Artemisia annua

substrate_name: kaempferol

enzyme: flavonoid 4'-O-methyltransferase

substrate: kaempferol


enzyme_display_name: [methyl-Co(III) methanol-specific corrinoid protein]:coenzyme M

organism: Methanosarcina barkeri


organism: Methanosarcina barkeri (strain Fusaro / DSM 804)

substrate_name: dimethylamine

smiles: CNC

substrate: dimethylamine


enzyme_display_name: mycinamicin III 3''-O-methyltransferase

substrate_name: mycinamicin III

sequence_source: uniprot_ec_organism_unique; direct reviewed UniProtKB accession check: Q49492

enzyme: mycinamicin III 3''-O-methyltransferase

substrate: mycinamicin III


enzyme_display_name: salicylate carboxymethyltransferase

organism: Clarkia breweri

substrate_name: salicylate

enzyme: salicylate carboxymethyltransferase

substrate: salicylate


organism: Pisum sativum


enzyme_display_name: phenylethanolamine N-methyltransferase

organism: Macaca mulatta

substrate_name: normetanephrine

enzyme: phenylethanolamine N-methyltransferase

substrate: normetanephrine


enzyme_display_name: selenocysteine Se-methyltransferase

organism: Astragalus bisulcatus

enzyme: selenocysteine Se-methyltransferase


enzyme_display_name: phenylpyruvate C(3)-methyltransferase

organism: Streptomyces hygroscopicus

enzyme: phenylpyruvate C(3)-methyltransferase


enzyme_display_name: emodin O-methyltransferase

organism: Aspergillus terreus

substrate_name: emodin

enzyme: emodin O-methyltransferase

substrate: emodin


enzyme_display_name: trans-anol O-methyltransferase

organism: Acorus calamus

substrate_name: resveratrol

enzyme: trans-anol O-methyltransferase

substrate: resveratrol


organism: Amycolatopsis mediterranei

substrate_name: 27-O-demethylrifamycin SV

substrate: 27-O-demethylrifamycin SV


enzyme_display_name: carminomycin 4-O-methyltransferase

organism: Streptomyces sp.

substrate_name: carminomycin

enzyme: carminomycin 4-O-methyltransferase

substrate: carminomycin


enzyme_display_name: mitomycin 6-O-methyltransferase

organism: Streptomyces lavendulae

proof_lines:
pH 7.4, 30°C
Raw SKiD Env: Temp=30°C, pH=7.4

enzyme: mitomycin 6-O-methyltransferase


organism: Saccharomyces cerevisiae


enzyme_display_name: type I protein arginine methyltransferase

organism: Homo sapiens

substrate_name: AdoMet

substrate: AdoMet


organism: Rattus norvegicus

enzyme: type I protein arginine methyltransferase


organism: Sphingobium sp.

substrate_name: vanillate

sequence_source: UniProtKB reviewed accession G2IQS7; canonical sequence preserved in the selected batch

substrate: vanillate


enzyme_display_name: mRNA m(6)A methyltransferase

enzyme: mRNA m(6)A methyltransferase


organism: Mycobacterium abscessus

sequence_source: UniProtKB reviewed accession B1MCE2 fetched by direct accession lookup


organism: Eubacterium limosum

substrate_name: L-carnitine

substrate: L-carnitine


substrate_name: gamma-butyrobetaine

substrate: gamma-butyrobetaine


enzyme_display_name: norajmaline N-methyltransferase

organism: Rauvolfia serpentina

substrate_name: norajmaline

enzyme: norajmaline N-methyltransferase

substrate: norajmaline


enzyme_display_name: ajmaline N-methyltransferase

enzyme: ajmaline N-methyltransferase


enzyme_display_name: acetylserotonin O-methyltransferase

organism: Arabidopsis thaliana

substrate_name: N-acetylserotonin

enzyme: acetylserotonin O-methyltransferase

substrate: N-acetylserotonin


organism: Bos taurus

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


organism: Oncorhynchus tshawytscha


enzyme_display_name: mycothione reductase

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

enzyme: mycothione reductase

substrate: NADPH


organism: Oryza sativa

sequence_source: UniProtKB REST direct accession check for Q6ZD89 on 2026-06-24


enzyme_display_name: flavone 3'-O-methyltransferase

organism: Chrysosplenium americanum

substrate_name: quercetin

enzyme: flavone 3'-O-methyltransferase

substrate: quercetin


enzyme_display_name: Transferred entry: 2.1.1.354

substrate_name: S-Adenosyl-L-methionine

enzyme: Transferred entry: 2.1.1.354

substrate: S-Adenosyl-L-methionine


enzyme_display_name: thymidylate synthase

organism: Crithidia fasciculata

enzyme: thymidylate synthase


organism: Enterococcus faecalis

substrate_name: methylenetetrahydrofolate

substrate: methylenetetrahydrofolate


organism: Lactobacillus casei

substrate_name: dUMP

substrate: dUMP


enzyme_display_name: peroxidase

organism: Burkholderia pseudomallei

assay_conditions_summary: pH 4.5; 298.15 K; cofactor NADH

enzyme: peroxidase


enzyme_display_name: sterol 24-C-methyltransferase

organism: Glycine max

substrate_name: Cycloartenol; S-Adenosyl-L-methionine

sequence_source: UniProtKB Q43445 REST JSON, active TrEMBL entry for Glycine max; canonical sequence evidence is preserved in the selected batch.

enzyme: sterol 24-C-methyltransferase

substrate: Cycloartenol; S-Adenosyl-L-methionine


organism: Leishmania major


enzyme_display_name: isoflavone 4'-O-methyltransferase

organism: Glycyrrhiza echinata

enzyme: isoflavone 4'-O-methyltransferase


organism: Pisum sativum


organism: Medicago truncatula


enzyme_display_name: indolepyruvate C-methyltransferase

organism: Streptomyces griseus

substrate_name: indolepyruvate (at 30 degrees Celsius and pH 7.6)

enzyme: indolepyruvate C-methyltransferase


organism: Mus musculus

substrate_name: S-methyl-L-methionine

substrate: S-methyl-L-methionine


organism: Rattus norvegicus

substrate_name: L-homocysteine

sequence_source: UniProtKB O09171 direct lookup

substrate: L-homocysteine


organism: Sus scrofa

sequence_source: UniProtKB Q95332 direct lookup; entry flagged Fragment


substrate_name: glycine betaine

substrate: glycine betaine


enzyme_display_name: putrescine N-methyltransferase

organism: Datura stramonium

enzyme: putrescine N-methyltransferase


organism: Solanum lycopersicum

substrate_name: putrescine

substrate: putrescine


enzyme_display_name: mRNA (guanine-N(7))-methyltransferase

organism: Encephalitozoon cuniculi (strain GB-M1)

substrate_name: GTP

enzyme: mRNA (guanine-N(7))-methyltransferase

substrate: GTP


organism: Saccharomyces cerevisiae

substrate_name: GpppA

substrate: GpppA


enzyme_display_name: methyltransferase cap1

organism: Homo sapiens

enzyme: methyltransferase cap1


organism: Orthopoxvirus vaccinia


enzyme_display_name: catechol O-methyltransferase

substrate_name: S-Adenosyl-L-methionine; L-Adrenaline

enzyme: catechol O-methyltransferase

substrate: S-Adenosyl-L-methionine; L-Adrenaline


organism: Sus scrofa domesticus


enzyme_display_name: calmodulin-lysine N-methyltransferase

enzyme: calmodulin-lysine N-methyltransferase


enzyme_display_name: nicotinate N-methyltransferase

substrate_name: nicotinate

enzyme: nicotinate N-methyltransferase

substrate: nicotinate


organism: Aquilegia coerulea

substrate_name: S-Adenosyl-L-methionine; Nicotinate

substrate: S-Adenosyl-L-methionine; Nicotinate


substrate_name: Nicotinate; S-Adenosyl-L-methionine

substrate: Nicotinate; S-Adenosyl-L-methionine


organism: Theobroma cacao


organism: Bos taurus

substrate_name: S-adenosyl-L-methionine

assay_conditions_summary: pH 10

substrate: S-adenosyl-L-methionine


organism: Arabidopsis thaliana


organism: Enterobacteria phage T4

substrate_name: S-adenosylmethionine

substrate: S-adenosylmethionine


organism: Escherichia phage T1


organism: Pyrococcus horikoshii


organism: Enterococcus faecalis


organism: Pinus massoniana


organism: Caenorhabditis elegans


enzyme_display_name: histamine N-methyltransferase

enzyme: histamine N-methyltransferase


organism: Cavia porcellus

substrate_name: histamine

substrate: histamine


organism: Mus musculus


organism: Rattus rattus

substrate_name: S-Adenosyl-L-methionine; Histamine

substrate: S-Adenosyl-L-methionine; Histamine


enzyme_display_name: apigenin 4'-O-methyltransferase

organism: Plagiochasma appendiculatum

substrate_name: apigenin

enzyme: apigenin 4'-O-methyltransferase

substrate: apigenin


organism: Rattus norvegicus


organism: Papaver somniferum

substrate_name: (S)-scoulerine

substrate: (S)-scoulerine


organism: Coptis japonica


enzyme_display_name: thiol S-methyltransferase

organism: Phaeodactylum tricornutum

substrate_name: thiocyanate

enzyme: thiol S-methyltransferase

substrate: thiocyanate


organism: Homo sapiens

substrate_name: hydrogen sulfide

smiles: S

substrate: hydrogen sulfide


enzyme_display_name: aminomethyltransferase

organism: Gallus gallus

substrate_name: tetrahydrofolate

enzyme: aminomethyltransferase

substrate: tetrahydrofolate


substrate_name: NAD+; Glycine; 5,6,7,8-Tetrahydrofolate

assay_conditions_summary: cofactor NAD+

substrate: NAD+; Glycine; 5,6,7,8-Tetrahydrofolate


organism: Oryctolagus cuniculus


organism: Pisum sativum


organism: Canis familiaris


organism: Mycobacterium tuberculosis


enzyme_display_name: methionyl-tRNA formyltransferase

organism: Enterococcus faecalis

enzyme: methionyl-tRNA formyltransferase


organism: Escherichia coli (strain K12)


enzyme_display_name: D-alanine 2-hydroxymethyltransferase

organism: Ensifer sp. AJ110404

smiles: CC(CO)(C(=O)O)N

enzyme: D-alanine 2-hydroxymethyltransferase


organism: Streptomyces niveus

substrate_name: decarbamoylnovobiocin

substrate: decarbamoylnovobiocin


enzyme_display_name: acetyl-CoA carboxytransferase

substrate_name: malonyl-CoA

enzyme: acetyl-CoA carboxytransferase

substrate: malonyl-CoA


enzyme_display_name: aspartate carbamoyltransferase

organism: Methanocaldococcus jannaschii

substrate_name: L-aspartate

sequence_source: UniProtKB Q58976 direct accession lookup; entry PYRB_METJA; reviewed Swiss-Prot; fetched 2026-06-03.

proof_lines:
Raw SKiD Env: Temp=37°C, pH=-----
Raw SKiD Env: Temp=55°C, pH=-----
Raw SKiD Env: Temp=15°C, pH=-----

enzyme: aspartate carbamoyltransferase

substrate: L-aspartate


organism: Pyrococcus abyssi

substrate_name: Carbamoyl phosphate

sequence_source: UniProtKB P77918 direct accession lookup; entry PYRB_PYRAB; reviewed Swiss-Prot; fetched 2026-06-03.

proof_lines:
Raw SKiD Env: Temp=37°C, pH=-----
Raw SKiD Env: Temp=30°C, pH=-----
Raw SKiD Env: Temp=55°C, pH=-----

substrate: Carbamoyl phosphate


enzyme_display_name: ornithine carbamoyltransferase

organism: Bos taurus

substrate_name: ornithine

smiles: C(CC(C(=O)O)N)CN

proof_lines:
at pH 7.2
at pH 8.5

enzyme: ornithine carbamoyltransferase

substrate: ornithine


substrate_name: L-citrulline

smiles: C(CC(C(=O)O)N)CNC(=O)N

proof_lines:
at 25°C and pH 8.4
at 25°C and pH 7.1

substrate: L-citrulline


substrate_name: Phosphate

smiles: O=P(O)(O)O

substrate: Phosphate


substrate_name: Carbamoyl phosphate; L-Ornithine

substrate: Carbamoyl phosphate; L-Ornithine


substrate_name: L-Citrulline

substrate: L-Citrulline


substrate_name: L-Ornithine; Carbamoyl phosphate

substrate: L-Ornithine; Carbamoyl phosphate


smiles: NC(=O)OP(=O)(O)O


substrate_name: L-Ornithine

substrate: L-Ornithine


organism: Mycoplasma arthritidis

substrate_name: L-Citrulline; Phosphate

substrate: L-Citrulline; Phosphate


organism: Pseudomonas aeruginosa


organism: Agrobacterium tumefaciens


organism: Pseudomonas syringae phaseolicola


enzyme_display_name: putrescine carbamoyltransferase

substrate_name: putrescine

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature Y230V/G231S/L232M/Y233G to reconstruct the assayed variant sequence.

smiles: C(CCN)CN

proof_lines: recombinant PTC mutant Y230V/G231S/L232M/Y233G, pH 8.5, 37°C

enzyme: putrescine carbamoyltransferase

substrate: putrescine


organism: Sus scrofa


enzyme_display_name: transketolase

organism: Cyberlindnera jadinii

substrate_name: thiamine diphosphate

enzyme: transketolase

substrate: thiamine diphosphate


organism: Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

substrate_name: fructose 6-phosphate (at pH 7.0 and 37 degrees Celsius)

smiles: C(C(C(C(C(=O)CO)O)O)O)OP(=O)(O)O

assay_conditions_summary: pH 7


organism: Homo sapiens

substrate_name: D-Ribose 5-phosphate; D-Xylulose 5-phosphate


organism: Leishmania mexicana

proof_lines:
pH 7.5
Raw SKiD Env: Temp=-----, pH=7.5
Raw SKiD Env: Temp=-----, pH=-----


organism: Saccharomyces cerevisiae

substrate_name: D-Ribose 5-phosphate

substrate: D-Ribose 5-phosphate


organism: Rattus norvegicus

substrate: D-Ribose 5-phosphate; D-Xylulose 5-phosphate


substrate_name: D-Xylulose 5-phosphate

substrate: D-Xylulose 5-phosphate


organism: Rhodobacter sphaeroides


organism: Scheffersomyces stipitis


substrate_name: Hydroxypyruvate

substrate: Hydroxypyruvate


substrate_name: sedoheptulose 7-phosphate

substrate: sedoheptulose 7-phosphate


organism: Spinacia oleracea

substrate_name: D-ribose 5-phosphate

substrate: D-ribose 5-phosphate


substrate_name: xylulose 5-phosphate

smiles: C(C(C(C(=O)CO)O)O)OP(=O)(O)O

substrate: xylulose 5-phosphate


enzyme_display_name: ubiquinol oxidase (non-electrogenic)

organism: Arabidopsis thaliana

enzyme: ubiquinol oxidase (non-electrogenic)


enzyme_display_name: lignostilbene alphabeta-dioxygenase

organism: Sphingomonas paucimobilis

enzyme: lignostilbene alphabeta-dioxygenase


enzyme_display_name: flavanone 3-dioxygenase

organism: Oryza sativa subsp. japonica

substrate_name: S-eriodictyol

enzyme: flavanone 3-dioxygenase

substrate: S-eriodictyol


enzyme_display_name: vinorine hydroxylase

organism: Rauvolfia serpentina

substrate_name: vinorine

enzyme: vinorine hydroxylase

substrate: vinorine


enzyme_display_name: methyl farnesoate epoxidase

organism: Diploptera punctata

substrate_name: methyl (2E,6E)-farnesoate (with purified enzyme)

enzyme: methyl farnesoate epoxidase


enzyme_display_name: (E)-2-methylbutanal oxime monooxygenase

organism: Manihot esculenta

substrate_name: (Z)-2-methylbutanal oxime

enzyme: (E)-2-methylbutanal oxime monooxygenase

substrate: (Z)-2-methylbutanal oxime


enzyme_display_name: methanethiol oxidase

substrate_name: methanethiol

enzyme: methanethiol oxidase

substrate: methanethiol


organism: Hyphomicrobium sp


organism: Mus musculus


enzyme_display_name: sphingoid base N-palmitoyltransferase

substrate_name: sphinganine (d18:0)

enzyme: sphingoid base N-palmitoyltransferase

substrate: sphinganine (d18:0)


substrate_name: sphinganine

substrate: sphinganine


enzyme_display_name: laccase

organism: Bacillus sp. SL-1

substrate_name: Syringaldazine; O2

sequence_source: UniProtKB A0A165W3F6 canonical FASTA fetched during exact accession lookup; UniProt entry is Laccase, EC 1.10.3.2, Bacillus sp. SL-1, sequence length 513.

assay_conditions_summary: buffer conditions and no primary measurement location

enzyme: laccase

substrate: Syringaldazine; O2


organism: Bacteroides thetaiotaomicron


substrate_name: Syringaldazine

substrate: Syringaldazine


organism: Escherichia coli


organism: Fomitopsis pinicola

substrate_name: guaiacol

sequence_source: UniProtKB:S8FGV1 direct FASTA; reviewed Swiss-Prot laccase EC 1.10.3.2; PMID:34116754 reference.

assay_conditions_summary: pH 5; 303.15 K; buffer sodium-citrate

substrate: guaiacol


organism: basidiomycete PM1

sequence_source: UniProt REST direct accession lookup, Q12571, 2026-05-24


organism: Lentinus brumalis

assay_conditions_summary: pH 4; 303.15 K; buffer their

proof_lines:
in McIlvaine buffer (pH 4.0), at 30°C
in McIlvaine buffer (pH 5.0), at 30°C


organism: Meiothermus ruber


organism: Caldalkalibacillus thermarum

substrate_name: O2; 2,6-Dimethoxyphenol

substrate: O2; 2,6-Dimethoxyphenol


organism: Aquifex aeolicus


organism: Phoma sp.

substrate_name: syringaldazine

substrate: syringaldazine


organism: Pseudomonas putida

sequence_source: UniProtKB exact accession Q88C03 REST lookup, retrieved 2026-07-01


enzyme_display_name: trans-4-coumaroyl-CoA 2'-hydroxylase

organism: Ipomoea batatas

substrate_name: feruloyl-CoA

assay_conditions_summary: pH 7.2; cofactor CoA

proof_lines: kcat is 0.28 sec(-1) with feruloyl-CoA as substrate. kcat is 0.28 sec(-1) with p-Coumaroyl-CoA as substrate.

enzyme: trans-4-coumaroyl-CoA 2'-hydroxylase

substrate: feruloyl-CoA


proof_lines: kcat is 0.35 sec(-1) with feruloyl-CoA as substrate. kcat is 0.33 sec(-1) with p-Coumaroyl-CoA as substrate.


proof_lines: kcat is 0.55 sec(-1) with feruloyl-CoA as substrate. kcat is 0.64 sec(-1) with p-Coumaroyl-CoA as substrate.


organism: Ruta graveolens

proof_lines: kcat is 0.46 sec(-1) with feruloyl-CoA as substrate (PubMed:22168819). kcat is 0.71 sec(-1) with p-coumaroyl-CoA as substrate (PubMed:22168819).


enzyme_display_name: methylphosphonate hydroxylase

organism: Gimesia maris (strain ATCC 29201 / DSM 8797 / 534-30)

substrate_name: methylphosphonate

proof_lines: kcat is 0.36 sec(-1) with methylphosphonate as substrate. kcat is 0.42 sec(-1) with 2-oxoglutarate as substrate.

enzyme: methylphosphonate hydroxylase

substrate: methylphosphonate


enzyme_display_name: methane monooxygenase (particulate)

organism: Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)

substrate_name: Unknown

assay_conditions_summary: cofactor NADH

proof_lines: Kinetic parameters have been established with the pMMO heteromeric complex.

enzyme: methane monooxygenase (particulate)

substrate: Unknown


enzyme_display_name: lupanine 17-hydroxylase (cytochrome c)

organism: Pseudomonas sp

substrate_name: sparteine (at 25 degrees Celsius and at pH 8.5)

proof_lines: kcat is 217 sec(-1) with lupanine as substrate and 11.8 sec(-1) with sparteine as substrate.

enzyme: lupanine 17-hydroxylase (cytochrome c)


enzyme_display_name: isopiperitenol dehydrogenase

organism: Mentha piperita

substrate_name: (-)-trans-carveol (at pH 7.5)

assay_conditions_summary: pH 7.5; cofactor NADP+

proof_lines: kcat is 0.02 sec(-1) with (-)-trans-carveol as substrate (at pH 7.5), kcat is 0.06 sec(-1) with (-)-trans-carveol as substrate (at pH 10), kcat is 0.002 sec(-1) with (-)-trans-isopiperitenol as substrate (at pH 7.5).

enzyme: isopiperitenol dehydrogenase

substrate: (-)-trans-carveol (at pH 7.5)


enzyme_display_name: tyramine N-methyltransferase

organism: Lophophora williamsii

substrate_name: mescaline

proof_lines: kcat is 0.0001 sec(-1) with mescaline as substrate (PubMed:37675639). kcat is 0.130 sec(-1) with 4-hydroxy-3,5-dimethoxyphenethylamine as substrate (PubMed:37675639).

enzyme: tyramine N-methyltransferase

substrate: mescaline


enzyme_display_name: trans-anol O-methyltransferase

organism: Pimpinella anisum

substrate_name: isoeugenol (at pH 7.5 and 25 degrees Celsius)

assay_conditions_summary: pH 7.5; buffer or captured segment for the populated kcat 0

enzyme: trans-anol O-methyltransferase


enzyme_display_name: phenylpyruvate C(3)-methyltransferase

organism: Streptomyces hygroscopicus

substrate_name: S-adenosyl-L-methionine

proof_lines: kcat is 0.8 sec(-1) with S-adenosyl-L-methionine as substrate. kcat is 8.15 sec(-1) with phenylpyruvic acid as substrate.

enzyme: phenylpyruvate C(3)-methyltransferase

substrate: S-adenosyl-L-methionine


organism: Pyrobaculum aerophilum

sequence_source: UniProtKB exact accession Q8ZWA8 REST lookup, retrieved 2026-07-01


organism: Streptomyces viridochromogenes

sequence_source: UniProtKB exact accession lookup J9S5G3, retrieved 2026-05-24


organism: Shiraia sp. SUPER-H168


organism: Streptomyces ipomoeae

sequence_source: UniProtKB REST A3QVH2, checked 2026-05-30


organism: Streptomyces coelicolor


organism: Corynebacterium glutamicum


organism: Streptomyces sviceus


organism: Mus musculus


enzyme_display_name: sphingoid base N-stearoyltransferase

enzyme: sphingoid base N-stearoyltransferase


enzyme_display_name: RING-type E3 ubiquitin transferase

organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: ATP

assay_conditions_summary: cofactor ATP

enzyme: RING-type E3 ubiquitin transferase

substrate: ATP


organism: Escherichia coli

substrate_name: UDP-Glc

substrate: UDP-Glc


enzyme_display_name: quinolinate synthase

organism: Bacillus subtilis (strain 168)

substrate_name: dihydroxyacetone phosphate

enzyme: quinolinate synthase

substrate: dihydroxyacetone phosphate


organism: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)


enzyme_display_name: pyridinol guanylyltransferase

organism: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

substrate_name: GTP

enzyme: pyridinol guanylyltransferase

substrate: GTP


organism: Cricetulus griseus

substrate_name: L-serine

substrate: L-serine


organism: Homo sapiens

substrate_name: serine (in the presence of 1 mM PE)

substrate: serine (in the presence of 1 mM PE)


substrate_name: serine (in the presence of 2 mM PC)

substrate: serine (in the presence of 2 mM PC)


enzyme_display_name: isoamyl acetate esterase

substrate_name: 3-methylbutyl acetate

enzyme: isoamyl acetate esterase

substrate: 3-methylbutyl acetate


enzyme_display_name: catechol oxidase

organism: Malus domestica

substrate_name: O2; Caffeoyl quinic acid

enzyme: catechol oxidase

substrate: O2; Caffeoyl quinic acid


organism: Bacillus licheniformis


organism: Geobacter metallireducens

sequence_source: UniProtKB:Q39TP1 direct FASTA; unreviewed TrEMBL multicopper oxidase type 2.

smiles: O=O


organism: Bacillus clausii

sequence_source: UniProtKB:Q5WEM6 canonical sequence retrieved by direct lookup on 2026-05-30


organism: Melanocarpus albomyces


organism: Halobacterium volcanii

sequence_source: UniProtKB direct lookup D4GPK6, reviewed Swiss-Prot entry LACC_HALVD


sequence_source: UniProtKB REST direct accession check on 2026-07-01: D4GPK6


organism: Meiothermus ruber

sequence_source: UniProtKB exact accession D3PLX1 REST lookup, retrieved 2026-07-01


enzyme_display_name: deacetoxycephalosporin-C synthase

organism: Streptomyces clavuligerus

substrate_name: ampicillin

enzyme: deacetoxycephalosporin-C synthase

substrate: ampicillin


proof_lines:
mutant C281Y, pH 7.4, 30°C
mutant enzyme C281Y


proof_lines:
mutant I305M, pH 7.4, 30°C
mutant enzyme I305M


substrate_name: Ampicillin

substrate: Ampicillin


substrate_name: Penicillin G

substrate: Penicillin G


proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutants R258H and R258A


substrate_name: 2-Oxoglutarate; O2; Penicillin G

proof_lines: recombinant His-tagged enzyme, pH 7.5, 30°C

proof_lines: recombinant FF2 derived from gene shuffling

proof_lines: recombinant clone FF8 derived from gene shuffling

substrate: 2-Oxoglutarate; O2; Penicillin G


proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258A

proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258A


proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258Q

proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258Q


proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258L

proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258L


substrate_name: penicillin G

proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258F

proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258F

substrate: penicillin G


proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutants R258A and R258Q


proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutants R258H and R258Q


proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutants R258A and R258F


proof_lines: pH 7.5, cosubstrate 2-oxoglutarate, recombinant wild-type enzyme


substrate_name: Penicillin N

substrate: Penicillin N


proof_lines:
mutant V275I, pH 7.4, 30°C
mutant enzyme V275I


proof_lines:
mutant enzyme L277Q
mutant L277Q


proof_lines:
mutant enzyme M188V
mutant M188V


proof_lines:
mutant enzyme C155Y
mutant C155Y


mutation_type: deletion


proof_lines: pH 7.5, cosubstrate 2-oxoglutarate, recombinant mutant R258K

proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258K

proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258K


proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutants R258L and R258F


proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258H

proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258H

proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutants R258H


proof_lines: pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutants R258K, R258A, and R258L


proof_lines: pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutants R258A and R258H


proof_lines:
mutant N304K, pH 7.4, 30°C
mutant enzyme N304K


proof_lines:
mutant V275I/I305M, pH 7.4, 30°C
mutant enzyme V275I/I305M


proof_lines:
mutant V275I7N304K, pH 7.4, 30°C
mutant C281Y7N304R, pH 7.4, 30°C


proof_lines:
mutant R306L, pH 7.4, 30°C
mutant R306L, 30°C


proof_lines:
mutant enzyme T91A
mutant T91A


proof_lines:
mutant enzyme H244Q
mutant H244Q


proof_lines:
mutant enzyme C155Y/Y184H/V275I/C281Y
mutant C155Y/Y184H/V275I/C281Y


proof_lines:
mutant enzyme Y184H
mutant Y184H


proof_lines: mutant enzyme C155Y/Y184H/V275I/C281Y/L305M, at pH 7.4 and 25°C


proof_lines: mutant enzyme C155Y/Y184H/V275I/C281Y/I305M/T213V/M73T, at pH 7.4 and 25°C


proof_lines: mutant enzyme C155Y/Y184H, at pH 7.4 and 25°C


enzyme_display_name: ferredoxin--NADP(+) reductase

organism: Anabaena sp.

substrate_name: reduced flavodoxin

assay_conditions_summary: pH 8; 286.15 K; cofactor NADPH

proof_lines:
pH 8.0, 13°C, wild-type enzyme
wild-type enzyme, pH 8.0
wild type enzyme of Anabaena sp.

enzyme: ferredoxin--NADP(+) reductase

substrate: reduced flavodoxin


substrate_name: NADH

assay_conditions_summary: cofactor NADP+

proof_lines: mutant T155G/A160T/L263P/Y303S, called PP3CT

substrate: NADH


proof_lines: mutant T155G/A160T/S223D/L263P/R264P/G265P, called AMP1PP5


organism: Plasmodium falciparum

substrate_name: NADPH

proof_lines: in an assay using K3Fe(CN)6 as electron acceptor for mutant H286K

proof_lines: in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286K

substrate: NADPH