substrate_name: GRSARVSTYA

assay_conditions_summary: pH 7

proof_lines: in presence of guanosine 5-3-O-(thio)triphosphate-Rac1, pH 7.0

proof_lines: in presence of phosphatidylserine, pH 7.0

substrate: GRSARVSTYA


enzyme_display_name: exoribonuclease H

organism: Human immunodeficiency virus 1

substrate_name: Unknown

source_db: brenda

kcat_display: Not reported

km_display: Not reported

kcat_over_km_display: Not reported

protein_accession_database: UniProt

source_license: CC BY 4.0

ki_unit: mM

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution

enzyme: exoribonuclease H

substrate: Unknown

source: brenda


mutation_signature: Unknown

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


enzyme_display_name: deoxyribonuclease IV

organism: Mycobacterium tuberculosis

substrate_name: 5'-end-labeled 29 nucleotide double-stranded DNA

ki_display: Not reported

km_unit: mM

enzyme: deoxyribonuclease IV


organism: Enterococcus faecalis


mutation_type: truncation

proof_lines: truncated enzyme containing an FN3 domain but not C-terminal domains including a CBM32 domain, at pH 6.0 and 50°C


enzyme_display_name: double-stranded uracil-DNA glycosylase

organism: Deinococcus radiodurans

substrate_name: uracil-mismatched double-stranded DNA

proof_lines: T/U containing substrate, wild-type, pH 5.5, 37°C

proof_lines: C/U containing substrate, wild-type, pH 5.5, 37°C

proof_lines: G/hmU containing substrate, wild-type, pH 5.5, 37°C

enzyme: double-stranded uracil-DNA glycosylase

substrate: uracil-mismatched double-stranded DNA


proof_lines: G/U containing substrate, mutant A50E, pH 5.5, 37°C


organism: Mycolicibacterium smegmatis

substrate_name: uracil-containing single stranded DNA

proof_lines: substrate contains A/U base pairs, mutant Q53A/H109A, pH 7.5, 37°C

proof_lines: substrate contains G/U base pairs, mutant Q53A/H109A, pH 7.5, 37°C

substrate: uracil-containing single stranded DNA


proof_lines: substrate contains A/U base pairs, mutant H109A, pH 7.5, 37°C

proof_lines: substrate contains G/U base pairs, mutant H109A, pH 7.5, 37°C


proof_lines: substrate contains G/U base pairs, mutant Q53A, pH 7.5, 37°C

proof_lines: substrate contains A/U base pairs, mutant Q53A, pH 7.5, 37°C


proof_lines: substrate contains A/U base pairs, mutant D56A, pH 7.5, 37°C

proof_lines: substrate contains G/U base pairs, mutant D56A, pH 7.5, 37°C


proof_lines: substrate contains A/U base pairs, mutant E52A, pH 7.5, 37°C

proof_lines: substrate contains G/U base pairs, mutant E52A, pH 7.5, 37°C


proof_lines: substrate contains A/U base pairs, wild-type, pH 7.5, 37°C

proof_lines: substrate contains G/U base pairs, wild-type, pH 7.5, 37°C


enzyme_display_name: site-1 protease

organism: Homo sapiens

proof_lines: pH and temperature not specified in the publication, recombinant mutant Y285A

enzyme: site-1 protease


proof_lines: pH and temperature not specified in the publication, recombinant wild-type enzyme


proof_lines: wild type enzyme, at pH 7.2, temperature not specified in the publication


substrate_name: Abz-VDVADQA-Dap(Dnp)

proof_lines: T7AC-tagged circularly permuted human enzyme, at pH 7.2, temperature not specified in the publication

substrate: Abz-VDVADQA-Dap(Dnp)


enzyme_display_name: (S)-2-methylmalate dehydratase

organism: Escherichia coli

substrate_name: fumarate

enzyme: (S)-2-methylmalate dehydratase

substrate: fumarate


substrate_name: mesaconate

substrate: mesaconate


organism: Paraburkholderia xenovorans


organism: Arabidopsis thaliana

source_license: CC BY 4.0; CC BY-NC-ND 4.0

source_databases_merged: brenda skid


source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)


organism: Plasmodium falciparum


enzyme_display_name: alanine racemase

organism: Bacillus subtilis

substrate_name: L-Alanine

enzyme: alanine racemase

substrate: L-Alanine


substrate_name: D-Alanine

source_db: oed

substrate: D-Alanine

source: oed


organism: Caldanaerobacter subterraneus subsp. tengcongensis

substrate_name: L-alanine

substrate: L-alanine


substrate_name: D-alanine

source_db: skid

source_license: CC BY-NC-ND 4.0

substrate: D-alanine

source: skid


organism: Candidatus Pelagibacter ubique


organism: Clostridioides difficile

proof_lines:
Raw SKiD Env: Temp=-----, pH=8.5
Raw SKiD Env: Temp=37°C, pH=7.5


source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0


organism: Geobacillus stearothermophilus


substrate_name: D-Lysine

substrate: D-Lysine


substrate_name: L-lysine

substrate: L-lysine


organism: Mycobacterium avium


organism: Pelagibacter ubique


organism: Pseudomonas aeruginosa

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

proof_lines: pH and temperature not specified in the publication


substrate_name: D-Ala

substrate: D-Ala


organism: Pseudomonas fluorescens


organism: Pyrococcus horikoshii


organism: Salmonella enteritidis


enzyme_display_name: glutamate racemase

organism: Lactiplantibacillus plantarum

substrate_name: D-glutamate

enzyme: glutamate racemase

substrate: D-glutamate


substrate_name: D-Glutamate

substrate: D-Glutamate


substrate_name: L-glutamate

substrate: L-glutamate


organism: Lactobacillus fermentum

substrate_name: L-Glutamate

substrate: L-Glutamate


organism: Mycobacterium tuberculosis


organism: Bacillus anthracis


assay_conditions_summary: pH 8; 298.15 K; cofactor NAD+

proof_lines: mutant T76A, at 25°C in 50 mM Tris-HCl (pH 8.0), 5 mM NAD+, 0.5 mM iodonitrotetrazolium chloride, 2.5 mM ADP, 20 units of L-glutamate dehydrogenase, 2 units of diaphorase and D-glutamate

proof_lines: mutant T76A, at 25 °C in 50 mM Tris-HCl, pH 8.0, 5 mM NAD+, 0.5 mM iodonitrotetrazolium chloride, 2.5 mM ADP, 20 units of L-glutamate dehydrogenase, 2 units of diaphorase and D-glutamate


substrate_name: Unknown

substrate: Unknown


proof_lines: wild type enzyme, at 25°C in 50 mM Tris-HCl, pH 8.0, 5 mM NAD+, 0.5 mM iodonitrotetrazolium chloride, 2.5 mM ADP, 20 units of L-glutamate dehydrogenase, 2 units of diaphorase and D-glutamate

proof_lines: wild type enzyme, 50 mM boric acid, 100 mM KCl, 0.2 mM dithiothreitol, pH 8.0, at 25°C in the presence of 0.22 microM glutamate racemase

proof_lines: wild type enzyme, 50 mM boric acid, 100 mM KCl, 0.2 mM dithiothreitol, pH 8.0 at 25°C in the presence of 0.22 microM glutamate racemase


assay_conditions_summary: pH 8; buffer potassium phosphate

proof_lines: His-tagged recombinant BsGR mutant V149A, in potassium phosphate buffer (10 mM, pH 8.0)


assay_conditions_summary: pH 8; 310.15 K; buffer potassium phosphate

proof_lines: His-tagged recombinant BsGR, in potassium phosphate buffer (10 mM, pH 8.0)


proof_lines: wild-type enzyme, pH and temperature not specified in the publication


organism: Enterococcus faecalis


organism: Escherichia coli


organism: Fusobacterium nucleatum subsp. nucleatum

proof_lines: His-tagged recombinant FnGR, in potassium phosphate buffer (10 mM, pH 8.0)


organism: Helicobacter pylori


organism: Streptococcus mutans


organism: Streptococcus pyogenes


organism: Thermotoga maritima

assay_conditions_summary: pH 8; 343.15 K; buffer recombinant His-tagged enzyme

proof_lines: recombinant His-tagged enzyme, pH 8.0, 70°C, bicine buffer

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C, bicine buffer


organism: Thermus thermophilus


organism: Wolbachia pipientis

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C


enzyme_display_name: UDP-glucose 4-epimerase

organism: Arabidopsis thaliana

substrate_name: UDPgalactose

enzyme: UDP-glucose 4-epimerase

substrate: UDPgalactose


substrate_name: UDP-D-galactose

assay_conditions_summary: pH 8.6; cofactor NAD+

proof_lines: recombinant enzyme UGE4, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-Glc dehydrogenase

proof_lines: recombinant enzyme UGE4, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase

substrate: UDP-D-galactose


proof_lines: recombinant enzyme UGE5, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase


proof_lines: recombinant Arabidopsis thaliana enzyme UGE1, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-Glc dehydrogenase

proof_lines: recombinant Arabidopsis thaliana enzyme UGE1, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase


proof_lines: recombinant enzyme UGE3, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-Glc dehydrogenase

proof_lines: recombinant enzyme UGE3, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase


proof_lines: recombinant Arabidopsis thaliana enzyme UGE2, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDPGlc dehydrogenase

proof_lines: recombinant Arabidopsis thaliana enzyme UGE2, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase


organism: Aspergillus nidulans

substrate_name: UDP-alpha-D-glucose

substrate: UDP-alpha-D-glucose


substrate_name: UDPglucose

substrate: UDPglucose


organism: Bos taurus


organism: Campylobacter jejuni subsp. jejuni


organism: Davallia divaricata

substrate_name: UDP-alpha-D-galactose

proof_lines: DdUGE1, pH and temperature not specified in the publication

substrate: UDP-alpha-D-galactose


proof_lines: DdUGE2, pH and temperature not specified in the publication


substrate_name: D-tagatose

substrate: D-tagatose


substrate_name: D-galactose

substrate: D-galactose


substrate_name: UDP-galactose

assay_conditions_summary: pH 7.5; 308.15 K; buffer McIlvaine

proof_lines:
in McIlvaine buffer (pH 7.5), at 35°C
Raw SKiD Env: Temp=35°C, pH=7.5

substrate: UDP-galactose


organism: Homo sapiens


organism: Hordeum vulgare

substrate_name: UDP-D-glucose

substrate: UDP-D-glucose


organism: Pyrococcus horikoshii


organism: Streptococcus thermophilus


substrate_name: UDP-N-acetyl-alpha-D-glucosamine

substrate: UDP-N-acetyl-alpha-D-glucosamine


substrate_name: UDP-N-acetyl-alpha-D-galactosamine

substrate: UDP-N-acetyl-alpha-D-galactosamine


proof_lines:
pH 7.0, 80°C
recombinant enzyme, pH 7.0, 80°C
Raw SKiD Env: Temp=80°C, pH=7


enzyme_display_name: actinidain

organism: Unknown

substrate_name: Nalpha-benzoyl-L-Arg p-nitroanilide

enzyme: actinidain

substrate: Nalpha-benzoyl-L-Arg p-nitroanilide


organism: Bacillus anthracis

proof_lines: isozyme RacE2, pH and temperature not specified in the publication

proof_lines: D-glutamate analog, good competitive inhibitor for RacE1

proof_lines: D-glutamate analog, only weak inhibitor of RacE2


organism: Lathyrus oleraceus

substrate_name: UDP-glucose

assay_conditions_summary: pH 8.6; 298.15 K; cofactor NAD+

proof_lines: 50 mM Tris-HCl buffer (pH 8.6), 0.1 mM NAD+, 1 mM UDP-sugar and enzyme

substrate: UDP-glucose


organism: Methanococcus maripaludis


organism: Pisum sativum


substrate_name: UDP-L-arabinose

substrate: UDP-L-arabinose


substrate_name: UDP-D-xylose

substrate: UDP-D-xylose


organism: Pseudomonas aeruginosa


organism: Pyrobaculum calidifontis

proof_lines:
in 100 mM MES-NaOH (pH 5.5), at 70°C
Raw SKiD Env: Temp=70°C, pH=5.5


enzyme_display_name: glutathione-disulfide reductase

organism: Plasmodium falciparum (isolate K1 / Thailand)

substrate_name: NADPH (at 25 degrees Celsius and pH 6.9)

source_db: uniprot

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

assay_conditions_summary: pH 6.9; cofactor NADPH

proof_lines: kcat is 188.3 sec(-1) with GSSG as substrate (at 25 degrees Celsius and pH 6.9).

enzyme: glutathione-disulfide reductase

source: uniprot


enzyme_display_name: reuteransucrase

organism: Limosilactobacillus reuteri

substrate_name: sucrose

proof_lines: hydrolysis activity, mutant enzyme A1066N/N1134S/N1135E/S1136V, at pH 4.7 and 50°C

proof_lines: total activity, mutant enzyme A1066N/N1134S/N1135E/S1136V, at pH 4.7 and 50°C

proof_lines: transferase activity, mutant enzyme A1066N/N1134S/N1135E/S1136V, at pH 4.7 and 50°C

enzyme: reuteransucrase

substrate: sucrose


proof_lines: hydrolysis activity, mutant enzyme P1026V/I1029V/A1066N, at pH 4.7 and 50°C

proof_lines: total activity, mutant enzyme P1026V/I1029V/A1066N, at pH 4.7 and 50°C

proof_lines: transferase activity, mutant enzyme P1026V/I1029V/A1066N, at pH 4.7 and 50°C


proof_lines: transferase activity, mutant enzyme A1066N, at pH 4.7 and 50°C

proof_lines: hydrolysis activity, mutant enzyme A1066N, at pH 4.7 and 50°C

proof_lines: total activity, mutant enzyme A1066N, at pH 4.7 and 50°C


proof_lines: hydrolysis activity, mutant enzyme P1026V/I1029V/A1066N/N1134S/N1135E/S1136V, at pH 4.7 and 50°C

proof_lines: total activity, mutant enzyme P1026V/I1029V/A1066N/N1134S/N1135E/S1136V, at pH 4.7 and 50°C

proof_lines: transferase activity, mutant enzyme P1026V/I1029V/A1066N/N1134S/N1135E/S1136V, at pH 4.7 and 50°C


enzyme_display_name: non-specific serine/threonine protein kinase

organism: Arabidopsis thaliana

substrate_name: Ser/Thr peptide 1 for the protein serine-threonine kinase activity

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


substrate_name: synthetic substrate

enzyme: non-specific serine/threonine protein kinase

substrate: synthetic substrate


enzyme_display_name: 4-hydroxyphenylpyruvate dioxygenase

substrate_name: 4-hydroxyphenylpyruvic acid

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

data_origin: experimental

enzyme: 4-hydroxyphenylpyruvate dioxygenase

substrate: 4-hydroxyphenylpyruvic acid


organism: Rattus norvegicus


organism: Plasmodium falciparum (isolate 3D7)

substrate_name: glutathione disulfide (GSSG) (at 25 degrees Celsius and pH 6.9)


substrate_name: ATP

assay_conditions_summary: cofactor ATP

substrate: ATP


substrate_name: histone H3 (at pH 7.5 and 30 degrees Celsius)


substrate_name: ATP with or without CaM

substrate: ATP with or without CaM


substrate_name: GLN1-1 (at pH 7.5 and 30 degrees Celsius)


proof_lines: hydrolysis activity, mutant enzyme N1134S/N1135E/S1136V, at pH 4.7 and 50°C

proof_lines: total activity, mutant enzyme N1134S/N1135E/S1136V, at pH 4.7 and 50°C

proof_lines: transferase activity, mutant enzyme N1134S/N1135E/S1136V, at pH 4.7 and 50°C


proof_lines: hydrolysis activity, mutant enzyme P1026V/I1029V, at pH 4.7 and 50°C

proof_lines: total activity, mutant enzyme P1026V/I1029V, at pH 4.7 and 50°C

proof_lines: transferase activity, mutant enzyme P1026V/I1029V, at pH 4.7 and 50°C


proof_lines: total activity, mutant enzyme H1065S/A1066S, at pH 4.7 and 50°C

proof_lines: hydrolysis activity, mutant enzyme H1065S/A1066S, at pH 4.7 and 50°C


proof_lines: hydrolysis activity, mutant enzyme I1029V, at pH 4.7 and 50°C

proof_lines: total activity, mutant enzyme I1029V, at pH 4.7 and 50°C

proof_lines: transferase activity, mutant enzyme I1029V, at pH 4.7 and 50°C


organism: Enterococcus faecalis


proof_lines: hydrolysis activity, wild type enzyme, at pH 4.7 and 50°C

proof_lines: total activity, wild type enzyme, at pH 4.7 and 50°C

proof_lines: transferase activity, wild type enzyme, at pH 4.7 and 50°C


proof_lines: total activity, mutant enzyme P1026V, at pH 4.7 and 50°C

proof_lines: transferase activity, mutant enzyme P1026V, at pH 4.7 and 50°C

proof_lines: hydrolysis activity, mutant enzyme P1026V, at pH 4.7 and 50°C


proof_lines: hydrolysis activity, mutant enzyme P1026V/I1029V/N1134S/N1135E/S1136V, at pH 4.7 and 50°C

proof_lines: total activity, mutant enzyme P1026V/I1029V/N1134S/N1135E/S1136V, at pH 4.7 and 50°C

proof_lines: transferase activity, mutant enzyme P1026V/I1029V/N1134S/N1135E/S1136V, at pH 4.7 and 50°C


organism: Homo sapiens

substrate_name: UDP-galactose


proof_lines: wild type enzyme, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 37°C

proof_lines: wild type enzyme, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 24°C


substrate_name: D-glutamate

assay_conditions_summary: pH 9.2; 298.15 K; cofactor NAD+

proof_lines: mutant Y221A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0

proof_lines: mutant Y221A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol

substrate: D-glutamate


substrate_name: L-glutamate

assay_conditions_summary: pH 8.2; 298.15 K; cofactor NAD+

proof_lines: mutant R25A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol

proof_lines: mutant R25A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.

substrate: L-glutamate


proof_lines: mutant R214A/K106A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase,

proof_lines: mutant R214A/K106A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol


proof_lines: mutant K29A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol

proof_lines: mutant K29A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.

proof_lines: mutant K29A, in 10 mM potassium phosphate (pH 8.2)and 0.2 mM dithiothreitol


enzyme_display_name: alkane 1-monooxygenase

organism: Oryctolagus cuniculus

substrate_name: laurate

proof_lines:
CYP4A4wt, omega-hydroxylation
CYP4A7wt, omega-hydroxylation

enzyme: alkane 1-monooxygenase

substrate: laurate


proof_lines: CYP4A7, W93S mutant, omega-hydroxylation


substrate_name: prostaglandin E1

proof_lines: CYP4A7, S255F mutant, omega-hydroxylation

substrate: prostaglandin E1


substrate_name: arachidonic acid

proof_lines: CYP4A7, H206Y mutant, omega-hydroxylation

substrate: arachidonic acid


proof_lines: CYP4A7, R90W mutant, omega-hydroxylation


proof_lines: CYP4A7, R90W/W93S mutant, omega-hydroxylation


proof_lines: CYP4A7, H206Y/S255F mutant, omega-hydroxylation


organism: Limosilactobacillus fermentum


organism: Marinithermus hydrothermalis

substrate_name: UDP-N-acetyl-alpha-D-glucosamine


proof_lines: pH 8, 30°C, mutant enzyme D36N and H152Q


organism: Aquifex pyrophilus


substrate_name: L-Glutamate

substrate: L-Glutamate


proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=-----, pH=8.5


organism: Bacillus anthracis

substrate_name: D-Glutamate

substrate: D-Glutamate


organism: Phocaeicola plebeius

proof_lines: truncated mutant enzyme D465S, at pH 7.0 and 37°C


proof_lines: truncated mutant enzyme D465A, at pH 7.0 and 37°C


proof_lines: truncated mutant enzyme D465G, at pH 7.0 and 37°C


proof_lines: wild type full-length enzyme, at pH 7.0 and 37°C

proof_lines: wild type truncated enzyme, at pH 7.0 and 37°C


organism: Helicobacter pylori


substrate_name: Unknown

proof_lines: value above 100 for deletion mutant E151K

substrate: Unknown


proof_lines: demonstrates a high degree of asymmetry in substrate processing with the Michaelis constant for D-glutamate approximately tenfold lower than for L-glutamate


substrate_name: lauric acid

substrate: lauric acid


organism: Escherichia coli

substrate_name: 21-mer C Grec

substrate: 21-mer C Grec


proof_lines: mutant H69A, pH 7.6, 37°C, in presence of Zn2+


organism: Saccoglossus kowalevskii

substrate_name: D-Aspartate

assay_conditions_summary: pH 8

proof_lines: mutant enzyme A156S, pH 8.0, temperature not specified in the publication, Asp/GluR enzyme

proof_lines: pH 8.0, temperature not specified in the publication, mutant enzyme A156S, Asp/GluR enzyme

substrate: D-Aspartate


proof_lines: mutant enzyme S156A, pH 8.0, temperature not specified in the publication, AspR enzyme


proof_lines: pH 8.0, temperature not specified in the publication, wild-type enzyme, Asp/GluR enzyme

proof_lines: wild-type enzyme, pH 8.0, temperature not specified in the publication, Asp/GluR enzyme

proof_lines: wild-type enzyme, pH 8.0, temperature not specified in the publication, AspR enzyme


organism: Aeromonas hydrophila

assay_conditions_summary: pH 8; 298.15 K; buffer 20 mM sodium phosphate

proof_lines: mutant enzyme Y149G, in 20 mM sodium phosphate buffer, pH 8.0, at 25°C

substrate: UDP-galactose


proof_lines: wild type recombinant enzyme, in 20 mM sodium phosphate buffer, pH 8.0, at 25°C


proof_lines: mutant enzyme K153N, in 20 mM sodium phosphate buffer, pH 8.0, at 25°C


enzyme_display_name: biphenyl 2,3-dioxygenase

organism: Paraburkholderia xenovorans

substrate_name: biphenyl

proof_lines: wild type enzyme, at 37°C, pH not specified in the publication

enzyme: biphenyl 2,3-dioxygenase

substrate: biphenyl


proof_lines: mutant enzyme S283M, at 37°C, pH not specified in the publication


substrate_name: UDP-alpha-D-glucose

proof_lines: pH 6.2, temperature not specified in the publication, recombinant wild-type enzyme

substrate: UDP-alpha-D-glucose


proof_lines: pH 6.2, temperature not specified in the publication, recombinant mutant Y149F


proof_lines: pH 6.2, temperature not specified in the publication, recombinant mutant K153M


enzyme_display_name: [myosin heavy-chain] kinase

organism: Acanthamoeba castellanii

substrate_name: GRGRSSVYS

assay_conditions_summary: pH 7; 303.15 K; cofactor ATP

proof_lines: recombinant wild-type catalytic domain, pH 7.0, 30°C

enzyme: [myosin heavy-chain] kinase

substrate: GRGRSSVYS


proof_lines: recombinant T632A mutant catalytic domain, pH 7.0, 30°C


organism: Pseudomonas sp.

assay_conditions_summary: 298.15 K; cofactor NADH


substrate_name: UDPgalactose

proof_lines: pH 6.2, temperature not specified in the publication, recombinant S124T

substrate: UDPgalactose


proof_lines: pH 6.2, temperature not specified in the publication, recombinant mutant S124A


substrate_name: DNA-RNA hybrid

substrate: DNA-RNA hybrid


organism: Comamonas testosteroni

proof_lines:
parental protein
recombinant, purified protein
recombinant His-tagged protein


organism: Ectopseudomonas oleovorans

proof_lines: Pseudomonas pseudoalcaligenes strain LB400

proof_lines: Pseudomonas pseudoalcaligenes strain 1072

proof_lines: Pseudomonas pseudoalcaligenes strain KF707


organism: Pandoraea pnomenusa


organism: Pusillimonas sp. T7-7

substrate_name: pentadecane

proof_lines: pH 7.5, 30°C, recombinant alkane hydroxylase large subunit

substrate: pentadecane


organism: Fusarium oxysporum


organism: Homo sapiens

substrate_name: palmitate

proof_lines:
omega-hydroxylation
(omega-1)-hydroxylation
gene CYP4A11

substrate: palmitate


organism: Pseudomonas putida

proof_lines: inhibits oxidation of methyl tert-butyl ether


enzyme_display_name: assimilatory sulfite reductase (NADPH)

substrate_name: sulfite

assay_conditions_summary: cofactor NADPH

proof_lines: cosubstrate 3-acetylpyridineadenine dinucleotide phosphate

enzyme: assimilatory sulfite reductase (NADPH)

substrate: sulfite


organism: Methanocaldococcus jannaschii

substrate_name: reduced F420

proof_lines: at a fixed sulfite concentration of 0.29 mM and within a reduced F420 concentration range of 0.004-0.06 mM

proof_lines: within a sulfite concentration range of 0.0014-0.3 mM and with a fixed reduced F420 concentration of 0.04 mM

substrate: reduced F420


organism: Saccharomyces bayanus


organism: Saccharomyces cerevisiae

substrate_name: NADPH

proof_lines:
reduction of cytochrome c
reduction of sulfite

substrate: NADPH


organism: Oryctolagus cuniculus

substrate_name: GSSG

substrate: GSSG


organism: Pteris vittata

assay_conditions_summary: pH 7; buffer 50 mM potassium phosphate; cofactor NADPH

proof_lines: in 50 mM potassium phosphate buffer (pH 7.0)


organism: Setaria cervi

assay_conditions_summary: pH 7; 295.15 K; buffer pH 7

proof_lines: in 50 mM potassium phosphate buffer pH 7.0, at 37°C


organism: Allochromatium vinosum

substrate_name: NADH

assay_conditions_summary: cofactor NADH

substrate: NADH


organism: Equus caballus

substrate_name: ferricyanide

substrate: ferricyanide


organism: Escherichia coli

substrate_name: glutathione disulfide

assay_conditions_summary: pH 7.5; cofactor NADPH

proof_lines: pH 7.5, temperature not specified in the publication

substrate: glutathione disulfide


enzyme_display_name: 1,3-beta-oligoglucan phosphorylase

organism: Euglena gracilis

substrate_name: phosphate

proof_lines:
pH 7.2, with laminaribiose
+ laminaribiose
pH 7.2, with laminaritetraose

enzyme: 1,3-beta-oligoglucan phosphorylase

substrate: phosphate


substrate_name: alpha-D-glucosamine 1-phosphate

proof_lines: with cellobiose as acceptor, at pH 7.0 and 37°C

proof_lines: with laminatriose as acceptor, at pH 7.0 and 37°C

substrate: alpha-D-glucosamine 1-phosphate


enzyme_display_name: 4,6-alpha-glucanotransferase (linear substrates/linear products)

organism: Limosilactobacillus reuteri

substrate_name: maltose

substrate: maltose


substrate_name: sucrose

proof_lines: His6-tagged enzyme, with maltose as cosubstrate, total activity, at pH 4.7 and 50°C

proof_lines: His6-tagged enzyme, tranferase activity, at pH 4.7 and 50°C

proof_lines: His6-tagged enzyme, total activity, at pH 4.7 and 50°C

substrate: sucrose


organism: Streptococcus thermophilus

substrate_name: amylose

substrate: amylose


organism: Acanthamoeba castellanii

substrate_name: GRGRSSVYS

proof_lines: phospholipid-bound, unphosphorylated enzyme, pH 7.0, 30°C

substrate: GRGRSSVYS


organism: Dictyostelium discoideum

substrate_name: RKKFGESEKTKTKEFL-amide

substrate: RKKFGESEKTKTKEFL-amide


enzyme_display_name: [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase

organism: Brassica oleracea

substrate_name: HMKSAMSGLHLVKRR

assay_conditions_summary: pH 7; 303.15 K; cofactor CoA

proof_lines:
pH 7.0, 30°C, kinase B
pH 7.0, 30°C, kinase A

substrate: HMKSAMSGLHLVKRR


organism: Rattus norvegicus

substrate_name: ATP

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

proof_lines:
pH 7.5, 37°C
pH 6.5, 30°C, low-MW kinase
in absence of AMP

enzyme: [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase

substrate: ATP


enzyme_display_name: exoribonuclease H

organism: Human immunodeficiency virus 1

substrate_name: poly(rA)n-poly(dT)n

proof_lines:
mutant p66C280P/p51C280P
mutant p66C280W/p51
mutant p66/p51C280P

enzyme: exoribonuclease H

substrate: poly(rA)n-poly(dT)n


substrate_name: DNA-16-nucleotide-RNA hybrid

proof_lines: pH 7.8, 37°C, recombinant wild-type enzyme

substrate: DNA-16-nucleotide-RNA hybrid


substrate_name: 18mer DNA-RNA hybrid

substrate: 18mer DNA-RNA hybrid


enzyme_display_name: venom exonuclease

organism: Walterinnesia aegyptia

substrate_name: Unknown

enzyme: venom exonuclease

substrate: Unknown


organism: Bothrops alternatus

substrate_name: bis(p-nitrophenyl)-phosphate

substrate: bis(p-nitrophenyl)-phosphate


organism: Bothrops atrox


organism: Bothrops jararacussu

substrate_name: bis(4-nitrophenyl) phosphate

substrate: bis(4-nitrophenyl) phosphate


organism: Crotalus adamanteus

substrate_name: uridine oligonucleotides

proof_lines: with different degrees of polymerization

proof_lines: depending on nucleobase and presence of Mg2+

substrate: uridine oligonucleotides


organism: Crotalus durissus terrificus


organism: Daboia siamensis

substrate_name: bis-p-nitrophenyl phosphate

substrate: bis-p-nitrophenyl phosphate


organism: Trimeresurus stejnegeri

substrate_name: nicotinamide adenine dinucleotide

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

substrate: nicotinamide adenine dinucleotide


organism: Vipera aspis

substrate_name: thymidine 5'-p-nitrophenyl phosphate

substrate: thymidine 5'-p-nitrophenyl phosphate


enzyme_display_name: deoxyribonuclease IV

organism: Thermococcus eurythermalis

substrate_name: ssDNA containing a (polyethylene glycol)3 spacer

enzyme: deoxyribonuclease IV


enzyme_display_name: double-stranded uracil-DNA glycosylase

organism: Escherichia coli

enzyme: double-stranded uracil-DNA glycosylase


enzyme_display_name: site-1 protease

organism: Homo sapiens

substrate_name: Ac-Arg-Ser-Leu-Lys-p-nitroanilide

proof_lines: in 25 mM Tris, 25 mM MES, 2 mM CaCl2, pH 7.4, at 37°C

enzyme: site-1 protease

substrate: Ac-Arg-Ser-Leu-Lys-p-nitroanilide


organism: Mammalia


organism: Bacteroides caccae

proof_lines: wild type truncated enzyme, at pH 7.0 and 37°C

proof_lines: wild type full-length enzyme, at pH 7.0 and 37°C


organism: Bombyx mori


enzyme_display_name: human endogenous retrovirus K endopeptidase

proof_lines: pH 5.0, 25°C, 13000 Da C-terminally truncated enzyme form


proof_lines:
pH 4.5, 18200 Da enzyme form
pH 4.5, 11600 Da core domain
pH 5.0, 25°C, 18000 Da enzyme form

enzyme: human endogenous retrovirus K endopeptidase


substrate_name: acetyl-VDVADGW-amide

substrate: acetyl-VDVADGW-amide


enzyme_display_name: actinidain

organism: Actinidia chinensis

substrate_name: benzyloxycarbonyl 4-nitrophenyl ester

proof_lines: above 0.25 mM, at 4.3% acetonitrile, 25°C, pH 5.3, parameter for pre-steady-state acylation phase

enzyme: actinidain

substrate: benzyloxycarbonyl 4-nitrophenyl ester


organism: Actinidia deliciosa

substrate_name: casein

proof_lines: 60°C, pH not specified in the publication

substrate: casein


enzyme_display_name: neutrophil collagenase

substrate_name: bovine collagen I

proof_lines: pH 7.0, 37°C, unprotonated and 1-protonated substrate

enzyme: neutrophil collagenase

substrate: bovine collagen I


enzyme_display_name: (S)-2-methylmalate dehydratase

organism: Aerobic bacterium

substrate_name: mesaconate

enzyme: (S)-2-methylmalate dehydratase

substrate: mesaconate


organism: Clostridium tetanomorphum

substrate_name: L-citramalate

substrate: L-citramalate


substrate_name: (S)-malate

substrate: (S)-malate


enzyme_display_name: glutamate racemase

organism: Enterococcus faecalis

substrate_name: D-glutamate

proof_lines: pH and temperature not specified in the publication

enzyme: glutamate racemase

substrate: D-glutamate


substrate_name: L-glutamate

proof_lines: in presence of 0.0065 mM of the activator UDP-N-acetylmuramyl-L-Ala

substrate: L-glutamate


organism: Fusobacterium nucleatum

substrate_name: D-homocysteic acid

substrate: D-homocysteic acid


organism: Lactiplantibacillus plantarum

substrate_name: D-Glu

substrate: D-Glu


enzyme_display_name: UDP-glucose 4-epimerase

substrate_name: UDP-glucose

proof_lines: fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker

enzyme: UDP-glucose 4-epimerase

substrate: UDP-glucose


organism: Galdieria sulphuraria

substrate_name: UDPgalactose

substrate: UDPgalactose


substrate_name: UDP-galactose

proof_lines: mutant enzyme M284K, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 24°C

proof_lines: mutant enzyme M284K, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 37°C

substrate: UDP-galactose


organism: Hordeum vulgare

substrate_name: UDP-Gal

substrate: UDP-Gal


organism: Levilactobacillus brevis


organism: Limosilactobacillus fermentum


organism: Mycobacterium tuberculosis

substrate_name: Unknown

substrate: Unknown


organism: Pediococcus pentosaceus

substrate_name: L-Glu

substrate: L-Glu


organism: Aeromonas hydrophila


organism: Arabidopsis thaliana

proof_lines: The approximate value is estimated from reaction velocities at saturating substrate concentrations (5–9 mM).

proof_lines: Different enzyme concentrations give slight variations in activity.

proof_lines: values are indirectly determined from the Haldane relationship


organism: Bifidobacterium bifidum

substrate_name: UDPglucose

substrate: UDPglucose


organism: Campylobacter jejuni

substrate_name: UDP-GlcNAc

substrate: UDP-GlcNAc


organism: Thermus thermophilus

assay_conditions_summary: pH 8.9; 323.15 K; cofactor NAD+

proof_lines: recombinant enzyme, 100 mM glycine-NaOH (pH 8.9), 4 mM UDP-galactose, 1 mM beta-NAD+, 8.3 mM MgCl2, and 5.4 U of UDP-glucose dehydrogenase


organism: Torulopsis candida


organism: Vicia faba


organism: Kluyveromyces marxianus

proof_lines:
native and renatured enzyme
25°C, pH 8.8


organism: Magallana gigas

substrate_name: UDP-alpha-D-galactose

substrate: UDP-alpha-D-galactose


organism: Poterioochromonas malhamensis


organism: Saccharomyces cerevisiae


enzyme_display_name: glutathione-disulfide reductase

organism: Bos taurus

substrate_name: H+; NADPH; Glutathione disulfide

assay_conditions_summary: pH 7; 303.15 K; cofactor NADPH

proof_lines:
calf liver enzyme, pH 7.0, 30°C
pH 7.4, 37°C
erythrocyte enzyme, pH 7.0, 30°C

enzyme: glutathione-disulfide reductase

substrate: H+; NADPH; Glutathione disulfide


organism: Euglena gracilis

substrate_name: H+; NADPH; Oxidized glutathione

substrate: H+; NADPH; Oxidized glutathione


organism: Enterococcus faecium

substrate_name: L-Glutamate

substrate: L-Glutamate


organism: Staphylococcus aureus


enzyme_display_name: alanine racemase

organism: Enterococcus faecalis

substrate_name: D-Alanine

enzyme: alanine racemase

substrate: D-Alanine


organism: Streptomyces lavendulae


proof_lines:
liver enzyme
mammary enzyme


organism: Pinus sylvestris

substrate_name: UDP-D-galactose

substrate: UDP-D-galactose


organism: Populus canadensis


enzyme_display_name: S-ribosylhomocysteine lyase

organism: Escherichia coli

substrate_name: S-Ribosylhomocysteine

enzyme: S-ribosylhomocysteine lyase

substrate: S-Ribosylhomocysteine


substrate_name: S-ribosylhomocysteine

proof_lines:
Co2+ substitued enzyme
Co2+ substituted enzyme

substrate: S-ribosylhomocysteine


substrate_name: S-Ribosyl-L-homocysteine

substrate: S-Ribosyl-L-homocysteine


organism: Streptococcus suis


enzyme_display_name: progesterone 11alpha-monooxygenase

organism: Priestia megaterium

substrate_name: progesterone

proof_lines: pH not specified in the publication, 30°C, mutant A243V/A395W/G397K

enzyme: progesterone 11alpha-monooxygenase

substrate: progesterone


proof_lines: pH not specified in the publication, 30°C, mutant A395W/G397K


proof_lines: pH not specified in the publication, 30°C, mutant T248V/A395I


proof_lines: pH not specified in the publication, 30°C, mutant A106T/A395W/G379K/R409L


proof_lines: pH not specified in the publication, 30°C, mutant M155I/F165L/A395I


organism: Pseudomonas savastanoi pv. phaseolicola

smiles: O=C(O)CCCC(=O)C(=O)O


substrate_name: L-arginine

proof_lines: with 2-oxoadipate as cosubstrate, at pH 7.5 and 25°C

substrate: L-arginine


smiles: O=C(O)CCC(=O)C(=O)O


substrate_name: L-Arginine

substrate: L-Arginine


organism: Pseudomonas syringae


organism: Homo sapiens

substrate_name: uracil in ASH1 E3 mRNA

substrate: uracil in ASH1 E3 mRNA


enzyme_display_name: retroviral ribonuclease H

organism: Human immunodeficiency virus 1

substrate_name: Unknown

proof_lines: presence of Mg2+ and DNA:RNA hybrid, pH 8.0

enzyme: retroviral ribonuclease H

substrate: Unknown


enzyme_display_name: chymase

substrate_name: Angiotensin I; H2O

enzyme: chymase

substrate: Angiotensin I; H2O


enzyme_display_name: gelatinase B

proof_lines:
pH 7.5, 37°C, monomeric form
pH 7.5, 37°C, dimeric form

enzyme: gelatinase B


organism: Bacillus subtilis


proof_lines:
Fe2+ containing BsLuxS
Zn2+ substituted BsLuxS
Co2+ substituted BsLuxS


proof_lines: pH not specified in the publication, 30°C, mutant A395I


proof_lines: pH not specified in the publication, 30°C, mutant T247W/A395W/G379K


proof_lines: pH not specified in the publication, 30°C, mutants A395II


proof_lines: pH not specified in the publication, 30°C, mutant T89N/A395I


proof_lines: pH not specified in the publication, 30°C, mutant T247V/A395I


proof_lines: pH not specified in the publication, 30°C, mutant A243V/A395I


proof_lines: pH not specified in the publication, 30°C, wild-type


proof_lines: pH not specified in the publication, 30°C, mutant A106T/A395I


proof_lines: pH not specified in the publication, 30°C, mutants A395I and M155I/F165L/A395I


proof_lines: pH not specified in the publication, 30°C, mutant D217V/A395I


proof_lines: pH not specified in the publication, 30°C, mutant A106T/A395I/R409L


proof_lines: pH not specified in the publication, 30°C, mutant T89N/A106T/A395I/R409L


enzyme_display_name: ribose-5-phosphate isomerase

organism: Acetivibrio thermocellus

substrate_name: D-Psicose

enzyme: ribose-5-phosphate isomerase

substrate: D-Psicose


substrate_name: D-ribose

proof_lines:
pH 7.5, 65°C
Raw SKiD Env: Temp=65°C, pH=7.5
Raw SKiD Env: Temp=80°C, pH=7.5

substrate: D-ribose


organism: Brucella anthropi

substrate_name: L-rhamnose

substrate: L-rhamnose


substrate_name: D-allose

substrate: D-allose


proof_lines: recombinant mutant S9T/S98H/S134H, pH 7.5, 50Â°C


proof_lines: recombinant mutant S98H/S134H, pH 7.5, 50Â°C


proof_lines: pH 7.0, 45°C, recombinant wild-type enzyme

proof_lines: recombinant wild-type enzyme, pH 7.5, 50Â°C


proof_lines: pH 7.5, 45°C, recombinant mutant A10T/T32S/G101N


proof_lines: pH 7.5, 45°C, recombinant wild-type enzyme


substrate_name: D-psicose

substrate: D-psicose


organism: Clostridioides difficile

substrate_name: L-talose

substrate: L-talose


substrate_name: D-Ribose 5-phosphate

substrate: D-Ribose 5-phosphate


substrate_name: D-ribose 5-phosphate

substrate: D-ribose 5-phosphate


organism: Mycobacterium tuberculosis


substrate_name: D-Allose 6-phosphate

substrate: D-Allose 6-phosphate


proof_lines: pH 7.5, 37°C, RpiB, substrate is D-ribose 5-phosphate


organism: Pyrococcus horikoshii


substrate_name: D-ribulose 5-phosphate

proof_lines:
50°C, pH 6.0, wild-type enzyme
93°C, pH 6, wild-type enzyme

substrate: D-ribulose 5-phosphate


proof_lines:
Raw SKiD Env: Temp=50°C, pH=6
Raw SKiD Env: Temp=93°C, pH=6


organism: Saccharomyces cerevisiae


organism: Klebsiella aerogenes


organism: Leishmania donovani


organism: Spinacia oleracea


organism: Thermotoga maritima


enzyme_display_name: dactylysin

organism: Unknown

substrate_name: Substance P

enzyme: dactylysin

substrate: Substance P


substrate_name: S-ribosylhomocysteine

substrate: S-ribosylhomocysteine


organism: Vibrio harveyi


proof_lines:
pH 8.0 and 70°C
pH 8.0, 70°C
Raw SKiD Env: Temp=70°C, pH=8


organism: Trypanosoma cruzi

substrate_name: D-Ribose 5-diphosphate

substrate: D-Ribose 5-diphosphate


proof_lines: in 50 mM Tris-HCl (pH 7.6), 150 mM NaCl and 5 mM MESNA, at 30°C


substrate_name: RNA-DNA hybrid

substrate: RNA-DNA hybrid


enzyme_display_name: [beta-adrenergic-receptor] kinase

organism: Bos taurus

substrate_name: ATP

assay_conditions_summary: pH 7.8; 296.15 K; cofactor ATP

proof_lines: pH 7.8, 23°C, recombinant wild-type GRK2, with substrate tubulin

enzyme: [beta-adrenergic-receptor] kinase

substrate: ATP


proof_lines: pH 7.8, 23°C, recombinant GRK2 C221V mutant, with substrate tubulin


proof_lines: pH 7.8, 23°C, recombinant GRK2 L271G mutant, with substrate tubulin


proof_lines:
native enzyme
pH 7.0, 22°C


enzyme_display_name: hippurate hydrolase

organism: Campylobacter jejuni

substrate_name: hippurate

enzyme: hippurate hydrolase

substrate: hippurate


substrate_name: N-acetylglycine

proof_lines: pH 7.5, 37°C, purified recombinant wild-type enzyme

substrate: N-acetylglycine


proof_lines: pH 7.5, 37°C, native enzyme in cell extract


organism: Escherichia coli

substrate_name: uracil54 in unmodified E. coli tRNA1-Val


enzyme_display_name: lysine/arginine leucyltransferase

substrate_name: KQC-acrydonylalanine

proof_lines: amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, 4-bromo phenylalanine, Kcat apparent

enzyme: lysine/arginine leucyltransferase

substrate: KQC-acrydonylalanine


substrate_name: beta-adrenergic receptor

proof_lines: pH 7.5, 30°C, recombinant beta-ARK 1, rhodopsin as substrate

substrate: beta-adrenergic receptor


organism: Homo sapiens

substrate_name: GEGMDEMEFTEAESNMN

proof_lines: Km above 0.1 mM, pH and temperature not specified in the publication

substrate: GEGMDEMEFTEAESNMN


enzyme_display_name: ADP ribose phosphorylase

organism: Euglena gracilis

substrate_name: ADP-ribose

assay_conditions_summary: pH 7.8; 308.15 K; cofactor ADP

proof_lines:
pH 7.8, 35°C
pH 7.8, 30°C
ADP/phosphate exchange

enzyme: ADP ribose phosphorylase

substrate: ADP-ribose


organism: Human immunodeficiency virus 1

substrate_name: poly(rA)n-poly(dT)n

proof_lines:
37°C, mutant p66C280P/p51
37°C, mutant p66/p51C280P
37°C, mutant p66/p51C280W

substrate: poly(rA)n-poly(dT)n


substrate_name: Unknown

substrate: Unknown


substrate_name: vitronectin of MW 65000

proof_lines:
75000 MW form
pH 7.6, 25°C, recombinant enzyme

substrate: vitronectin of MW 65000


organism: Mus musculus

substrate_name: SGQPHYLTTA

proof_lines:
pH 7.5, 37°C, peptide B1
pH 7.5, 37°C, peptide A11m2
pH 7.5, 37°C, peptide A10

substrate: SGQPHYLTTA


enzyme_display_name: mitochondrial intermediate peptidase

proof_lines: in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25°C

enzyme: mitochondrial intermediate peptidase


substrate_name: atrial natriuretic peptide

substrate: atrial natriuretic peptide


organism: Xenopus laevis

substrate_name: dynorphin A

proof_lines:
[Leu5,Arg6]enkephalin
(6-11)substance P hexapeptide
70% inhibition when tested on kermit

substrate: dynorphin A


organism: Fusarium incarnatum


organism: Pseudomonas putida

substrate_name: N-benzoyl-L-alanine

substrate: N-benzoyl-L-alanine


organism: Acetivibrio thermocellus

substrate_name: L-ribulose

assay_conditions_summary: pH 7.5; 338.15 K; buffer 50 mM Tris-HCl

proof_lines: in 50 mM Tris-HCl buffer (pH 7.5), at 65°C

substrate: L-ribulose


substrate_name: D-ribose 5-phosphate

proof_lines:
calf spleen
ox muscle
calf liver

substrate: D-ribose 5-phosphate


organism: Clostridioides difficile

substrate_name: D-ribose

substrate: D-ribose


organism: Cyberlindnera jadinii

proof_lines:
enzyme form III
enzyme form II
enzyme form I


organism: Echinococcus granulosus


proof_lines: pH 7.5, 37°C, RpiB, substrate is D-ribose 5-phosphate


organism: Klebsiella aerogenes


organism: Bacillus subtilis


organism: Lactiplantibacillus plantarum

substrate_name: S-ribosylhomocysteine

proof_lines:
isoform LuxS1, pH 8.0, 45°C
isoform LuxS2, pH 8.0, 37°C

substrate: S-ribosylhomocysteine


organism: Thermus thermophilus


organism: Lathyrus oleraceus

substrate_name: D-ribulose 5-phosphate

substrate: D-ribulose 5-phosphate


organism: Mycobacterium tuberculosis


organism: Pediococcus pentosaceus


organism: Pseudothermotoga lettingae

substrate_name: D-psicose

substrate: D-psicose


organism: Rhodospirillum rubrum


organism: Saccharomyces cerevisiae


organism: Spinacia oleracea


organism: Thermotoga maritima

proof_lines:
at 60°C and pH 7.0
at 80°C and pH 7.0


organism: Vibrio harveyi

substrate_name: S-Ribosylhomocysteine

substrate: S-Ribosylhomocysteine


organism: Salmonella typhimurium

substrate_name: L-Alanine

substrate: L-Alanine


substrate_name: L-alanine

substrate: L-alanine


organism: Treponema denticola


substrate_name: D-Alanine

substrate: D-Alanine


substrate_name: L-Alanine methyl ester

substrate: L-Alanine methyl ester


organism: Wolbachia pipientis

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C


organism: Aquifex pyrophilus

substrate_name: L-glutamate

substrate: L-glutamate


organism: Acetivibrio thermocellus

substrate_name: D-Psicose

substrate: D-Psicose


organism: Streptococcus suis


organism: Vibrio cholerae

substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication

substrate: Unknown


proof_lines:
Co2+ substituted VhLuxS
Co2+ substituted enzyme


organism: Arabidopsis thaliana


organism: Escherichia coli


organism: Mycobacterium tuberculosis


organism: Plasmodium falciparum

proof_lines:
pH 7.0, 27°C
Raw SKiD Env: Temp=27°C, pH=7


organism: Salmonella enteritidis


organism: Aquifex aeolicus

substrate_name: S-adenosyl-L-methionine

proof_lines: wild-type, 55°C, pH not specified in the publication

substrate: S-adenosyl-L-methionine


proof_lines: mutant Y167F, 55°C, pH not specified in the publication


proof_lines: mutant T132S, 55°C, pH not specified in the publication


proof_lines: mutant E168A, 55°C, pH not specified in the publication


proof_lines: mutant T165A, 55°C, pH not specified in the publication


proof_lines: mutant H104A, 55°C, pH not specified in the publication


proof_lines: mutant R108A, 55°C, pH not specified in the publication


proof_lines: mutant D97A, 55°C, pH not specified in the publication


proof_lines: mutant Y95A, 55°C, pH not specified in the publication


substrate_name: guanine46 in m5U54-modified tRNAPhe

proof_lines: single turnover apparent rate, pH 7.5, 37°C

substrate: guanine46 in m5U54-modified tRNAPhe


organism: Saccharomyces cerevisiae

substrate_name: guanine46 in tRNA

mutation_type: deletion

proof_lines: pH 7.5, 30°C, tRNA variant with deletion of the anticodon-arm

substrate: guanine46 in tRNA


proof_lines: pH 7.5, 30°C, tRNA variant with deletion of the aminoacyl-stem


enzyme_display_name: iodide peroxidase

organism: Homo sapiens

substrate_name: guaiacol

proof_lines: wild-type, pH 7.4, temperature not specified in the publication

enzyme: iodide peroxidase

substrate: guaiacol


proof_lines: mutant P499L, pH 7.4, temperature not specified in the publication


proof_lines: mutant G387R, pH 7.4, temperature not specified in the publication


proof_lines: mutant C808R, pH 7.4, temperature not specified in the publication


proof_lines: mutant D74A, 55°C, pH not specified in the publication


proof_lines: mutant K101A, 55°C, pH not specified in the publication


proof_lines: mutant R107A, 55°C, pH not specified in the publication


proof_lines: mutant Y167A, 55°C, pH not specified in the publication


proof_lines: mutant T132C, 55°C, pH not specified in the publication


proof_lines: mutant E47A, 55°C, pH not specified in the publication


proof_lines: mutant T132A, 55°C, pH not specified in the publication


proof_lines: pH 7.5, 30°C, tRNA variant with substitution of C48 by A

proof_lines: pH 7.5, 30°C, tRNA variant with disruption of C13-G22-G46 tertiary base pair

proof_lines: pH 7.5, 30°C, tRNA variant with disruption of tertiary base pairs between D- and T-arms


organism: Cricetulus griseus


substrate_name: UDP-GlcA

substrate: UDP-GlcA


substrate_name: UDP-GlcNAc

substrate: UDP-GlcNAc


enzyme_display_name: mogroside IE synthase

organism: Siraitia grosvenorii

substrate_name: UDP-alpha-D-glucose

enzyme: mogroside IE synthase

substrate: UDP-alpha-D-glucose


substrate_name: mogrol

substrate: mogrol


organism: Staphylococcus aureus

substrate_name: RNA(n)

substrate: RNA(n)


organism: Staphylococcus epidermidis

substrate_name: ADP

assay_conditions_summary: pH 7.5; 299.15 K; cofactor ADP

proof_lines:
polyadenylation reaction, pH 7.5, 26°C
phosphorolysis reaction, pH 8.2, 37°C

substrate: ADP


enzyme_display_name: chondroitin 6-sulfotransferase

enzyme: chondroitin 6-sulfotransferase


organism: Mus musculus

substrate_name: 3'-phosphoadenylyl sulfate

proof_lines: pH 6.8, 37°C, recombinant mutant N457S, with chondroitin

substrate: 3'-phosphoadenylyl sulfate


proof_lines: pH 6.8, 37°C, recombinant mutant N413S, with keratan sulfate

proof_lines: pH 6.8, 37°C, recombinant mutant N413S, with chondroitin


proof_lines: pH 6.8, 37°C, recombinant mutant N250S, with chondroitin


proof_lines: pH 6.8, 37°C, recombinant wild-type enzyme, with chondroitin

proof_lines: pH 6.8, 37°C, recombinant wild-type enzyme, with keratan sulfate


enzyme_display_name: poly(ADP-ribose) glycohydrolase

organism: Deinococcus radiodurans

substrate_name: Poly(ADP-ribose)

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ADP

proof_lines: pH 7.5, 37°C, recombinant wild-type enzyme

enzyme: poly(ADP-ribose) glycohydrolase

substrate: Poly(ADP-ribose)


assay_conditions_summary: pH 7.2; 310.15 K; cofactor ADP

proof_lines: purified recombinant catalytic enzyme domain, in 50 mM KPO4 (pH 7.2), at 37°C


substrate_name: Alpha-NAD+

assay_conditions_summary: pH 7; 303.15 K; cofactor NAD+

substrate: Alpha-NAD+


enzyme_display_name: beta-galactofuranosidase

organism: Aspergillus nidulans

substrate_name: 4-nitrophenyl beta-D-galactofuranoside

enzyme: beta-galactofuranosidase

substrate: 4-nitrophenyl beta-D-galactofuranoside


enzyme_display_name: arginine deiminase

organism: Mycoplasma hominis

substrate_name: H2O; L-Arginine

enzyme: arginine deiminase

substrate: H2O; L-Arginine


organism: Pseudomonas aeruginosa


enzyme_display_name: aspartate racemase

organism: Acropora millepora

substrate_name: D-Aspartate

enzyme: aspartate racemase

substrate: D-Aspartate


organism: Bifidobacterium bifidum

substrate_name: L-Aspartate

substrate: L-Aspartate


organism: Crassostrea gigas


organism: Penaeus monodon


organism: Picrophilus torridus


organism: Pyrococcus horikoshii


organism: Aspergillus niger


organism: Streptomyces sp. JHA19


organism: Streptomyces sp. JHA26


enzyme_display_name: ginsenosidase type III

organism: Terrabacter ginsenosidimutans

substrate_name: ginsenoside Rb1

enzyme: ginsenosidase type III

substrate: ginsenoside Rb1


enzyme_display_name: ginsenoside Rb1 beta-glucosidase

organism: Pseudonocardia sp.

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

assay_conditions_summary: pH 7

enzyme: ginsenoside Rb1 beta-glucosidase

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Pseudothermotoga thermarum


organism: Thermotoga petrophila

assay_conditions_summary: pH 5


enzyme_display_name: prolyl oligopeptidase

organism: Bos taurus

substrate_name: H2O; Z-Gly-Pro-AMC

enzyme: prolyl oligopeptidase

substrate: H2O; Z-Gly-Pro-AMC


organism: Homo sapiens


enzyme_display_name: ureidoacrylate amidohydrolase

organism: Escherichia coli

enzyme: ureidoacrylate amidohydrolase


organism: Mycoplasma arginini


organism: Mycoplasma arthritidis


organism: Thermococcus litoralis


enzyme_display_name: 4-hydroxyproline epimerase

organism: Agrobacterium tumefaciens

enzyme: 4-hydroxyproline epimerase


organism: Agrobacterium vitis


organism: Bacillus cereus


organism: Bacillus thuringiensis


organism: Brucella abortus


organism: Brucella melitensis


organism: Brucella melitensis biotype 1


organism: Pyrococcus horikoshii


organism: Ochrobactrum anthropi


organism: Paracoccus denitrificans


organism: Pseudomonas putida


organism: Rhizobium etli


organism: Rhizobium meliloti


organism: Shewanella loihica


organism: Streptosporangium roseum


organism: Brucella suis


organism: Burkholderia multivorans


organism: Burkholderia pseudomallei


organism: Burkholderia thailandensis


organism: Chromobacterium violaceum


organism: Chromohalobacter salexigens


organism: Ferroplasma acidarmanus


organism: Labrenzia aggregata


organism: Labrenzia alexandrii


organism: Xanthomonas campestris


enzyme_display_name: L-arabinose isomerase

organism: Acidothermus cellulolyticus

substrate_name: L-arabinose

enzyme: L-arabinose isomerase

substrate: L-arabinose


organism: Alicyclobacillus acidocaldarius

proof_lines:
wild-type at pH 6 and 65°C
wild-type at pH 7 and 65°C
wild-type at pH 8 and 65°C


substrate_name: L-Arabinose

substrate: L-Arabinose


proof_lines:
mutant K269E at pH 7 and 65°C
mutant K269E at pH 8 and 65°C
mutant K269E at pH 6 and 65°C


proof_lines:
pH 6.0, 65Â°C, recombinant enzyme
pH 7.5, 75Â°C, recombinant enzyme
Raw SKiD Env: Temp=65°C, pH=6


organism: Alicyclobacillus hesperidum

substrate_name: D-galactose

substrate: D-galactose


organism: Anoxybacillus flavithermus


assay_conditions_summary: pH 9.5; 368.15 K; buffer 50 mM glycine-NaOH

proof_lines: without borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C

proof_lines: with 10 mM borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C


organism: Arthrobacter sp.


organism: Bacillus halodurans


organism: Bacillus licheniformis


proof_lines: pH 7.5, 50°C, recombinant wild-type enzyme


proof_lines: pH and temperature not specified in the publication


organism: Bacillus subtilis

substrate_name: L-ribulose

proof_lines: pH 7.5, 23°C, recombinant His6-tagged wild-type enzyme

substrate: L-ribulose


organism: Bacillus velezensis


organism: Bifidobacterium adolescentis


organism: Geobacillus stearothermophilus

substrate_name: L-Fucose

substrate: L-Fucose


proof_lines: without borate, pH 8.0, 60°C, recombinant wild-type enzyme

proof_lines: with borate, pH 8.0, 60°C, recombinant wild-type enzyme


proof_lines: without borate, pH 8.0, 60°C, recombinant mutant H18T

proof_lines: with borate, pH 8.0, 60°C, recombinant mutant H18T


organism: Geobacillus thermodenitrificans

proof_lines: in the presence of 10 mM Mn2+, enzyme treated with EDTA

proof_lines: in the presence of 1 mM Mn2+, enzyme treated with EDTA


organism: Bifidobacterium longum

proof_lines:
at pH 6.5 and 45°C
pH 6.0-6.5, 55Â°C, recombinant enzyme


organism: [Clostridium] hylemonae


organism: Enterococcus faecium

proof_lines: N-His-tagged recombinant protein, Michaelis-Menten kinetics, pH 5.6, 50°C

proof_lines: C-His-tagged recombinant protein, K0.5 value, cooperative interaction model, pH 5.6, 50°C

proof_lines: pH 7.0-7.5, 45-50Â°C, recombinant enzyme


organism: Geobacillus kaustophilus

proof_lines:
pH 7.5, 22°C
Raw SKiD Env: Temp=22°C, pH=7.5


proof_lines: pH 8.0, 60°C, recombinant enzyme expressed in Escherichia coli

proof_lines: pH 8.0, 60°C, recombinant enzyme expressed in Bacillus subtilis


proof_lines:
mutant C450S/N475K, pH 8, 55°C
C450S /N475K mutant


organism: Halalkalibacterium halodurans


organism: Heyndrickxia coagulans


organism: Limosilactobacillus fermentum

proof_lines:
mutant enzyme D299R, at pH 4.0 and 65°C
mutant enzyme D299R, at pH 6.0 and 65°C
mutant enzyme D299R, at pH 5.0 and 65°C


proof_lines:
wild type enzyme, at pH 6.0 and 65°C
wild type enzyme, at pH 5.0 and 65°C


proof_lines:
mutant enzyme D269K, at pH 5.0 and 65°C
mutant enzyme D269K, at pH 4.0 and 65°C
mutant enzyme D269K, at pH 6.0 and 65°C


proof_lines: mutant enzyme D269K/D299K, at pH 5.0 and 65°C

proof_lines: mutant enzyme D269K/D299K, at pH 6.0 and 65°C

proof_lines: mutant enzyme D269K/D299K, at pH 4.0 and 65°C


proof_lines: mutant enzyme D268K/D269K/D299K, at pH 6.0 and 65°C

proof_lines: mutant enzyme D268K/D269K/D299K, at pH 4.0 and 65°C

proof_lines: mutant enzyme D268K/D269K/D299K, at pH 5.0 and 65°C


proof_lines:
mutant enzyme D299K, at pH 5.0 and 65°C
mutant enzyme D299K, at pH 6.0 and 65°C
mutant enzyme D299K, at pH 4.0 and 65°C


proof_lines:
mutant enzyme D268K, at pH 5.0 and 65°C
mutant enzyme D268K, at pH 6.0 and 65°C
mutant enzyme D268K, at pH 4.0 and 65°C


proof_lines: mutant enzyme D268K/D299K, at pH 6.0 and 65°C

proof_lines: mutant enzyme D268K/D299K, at pH 5.0 and 65°C

proof_lines: mutant enzyme D268K/D299K, at pH 4.0 and 65°C


assay_conditions_summary: pH 5; 338.15 K; buffer context

proof_lines: in 50 mM phosphate buffer (pH 6.5), 1mM Mn2+, 2mM Co2+, at 65°C


organism: Limosilactobacillus reuteri subsp. rodentium


organism: Parageobacillus thermoglucosidasius

proof_lines:
at pH 7.0 and 40°C
pH 7.0, 40Â°C, recombinant enzyme


organism: Pediococcus pentosaceus


organism: Lactiplantibacillus plantarum

proof_lines:
at 60°C, pH 7.5
pH 7.5, 60Â°C, recombinant enzyme


organism: Lactobacillus plantarum

substrate_name: D-Galactose

substrate: D-Galactose


organism: Lactobacillus sakei


organism: Latilactobacillus sakei subsp. sakei


organism: Lentilactobacillus parabuchneri


organism: Levilactobacillus brevis


proof_lines: mutant enzyme D268K/D269K, at pH 6.0 and 65°C

proof_lines: mutant enzyme D268K/D269K, at pH 4.0 and 65°C

proof_lines: mutant enzyme D268K/D269K, at pH 5.0 and 65°C


enzyme_display_name: 2-hydroxymuconate tautomerase

organism: Archaeoglobus fulgidus

proof_lines: pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate

enzyme: 2-hydroxymuconate tautomerase


organism: Bacillus subtilis


organism: Burkholderia lata


organism: Chloroflexus aurantiacus

substrate_name: phenylenolpyruvate

proof_lines: pH 7.3, 24°C, recombinant wild-type hh4-OT

substrate: phenylenolpyruvate


proof_lines: pH 7.3, 24°C, recombinant hh4-OT mutant betaR11A

proof_lines: pH 7.3, 24°C, recombinant hh4-OT mutant betaP1A

proof_lines: pH 7.3, 24°C, recombinant hh4-OT mutant alphaR40A


organism: Gammaproteobacteria bacterium


organism: Leptothrix cholodnii


assay_conditions_summary: pH 6


organism: Pseudoalteromonas haloplanktis


organism: Shewanella sp. ANA-3


proof_lines: pH 5.5-6.5, 15-35Â°C, recombinant enzyme


organism: Thermoanaerobacter mathranii

substrate_name: D-fucose

substrate: D-fucose


organism: Thermoanaerobacter mathranii subsp. mathranii

assay_conditions_summary: pH 8


organism: Thermoanaerobacterium saccharolyticum

assay_conditions_summary: pH 7.5; 333.15 K; buffer 25 mM Tris-HCl

proof_lines: in 25 mM Tris-HCl buffer (pH 7.5), at 60°C


organism: Thermotoga maritima


organism: Thermotoga neapolitana


organism: Methylibium petroleiphilum

assay_conditions_summary: pH 7.3; 297.15 K; buffer 10 mM potassium phosphate

proof_lines: isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C

proof_lines: isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C


organism: Pseudomonas putida

substrate_name: (2E)-2-Hydroxypenta-2,4-dienoic acid

substrate: (2E)-2-Hydroxypenta-2,4-dienoic acid


enzyme_display_name: isopentenyl-diphosphate Delta-isomerase

organism: Escherichia coli

substrate_name: isopentenyl diphosphate

enzyme: isopentenyl-diphosphate Delta-isomerase

substrate: isopentenyl diphosphate


proof_lines:
wild-type enzyme
wild-type, pH 7.4, 37°C


substrate_name: Isopentenyl diphosphate

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

proof_lines: pH and temperature not specified in the publication

source_databases_merged: sabio_rk brenda skid

substrate: Isopentenyl diphosphate


organism: Homo sapiens


organism: Saccharolobus shibatae

smiles: C=C(C)CCOP(=O)(O)OP(=O)(O)O


assay_conditions_summary: pH 6; 333.15 K; cofactor NADH

proof_lines: dehydrogenase reaction without isopentenyl diphosphate


organism: Streptomyces sp.


organism: Sulfolobus shibatae


organism: Saccharomyces cerevisiae


proof_lines: mutant enzyme L141H/Y195F/W256C, at pH 7.5 and 30°C


organism: Staphylococcus aureus


organism: Streptococcus pneumoniae


organism: Nepenthes x ventrata

substrate_name: fluorogenic FS6 peptide

proof_lines: kcat is 1.81 sec(-1) for the cleavage of the fluorogenic FS6 peptide (PubMed:35915115). kcat is 6.55 sec(-1) for the cleavage of the fluorogenic FS6-QPQL peptide (PubMed:35915115).

substrate: fluorogenic FS6 peptide


enzyme_display_name: acetylsalicylate deacetylase

organism: Rattus norvegicus

substrate_name: 2-Nitrophenyl butyrate

proof_lines:
pH 7.8, 25°C, isozyme I
pH 7.8, 25°C, isozyme II
pH 7.4, 37°C, isozyme I

enzyme: acetylsalicylate deacetylase

substrate: 2-Nitrophenyl butyrate


organism: Unknown


substrate_name: UDP-glucuronate

substrate: UDP-glucuronate


proof_lines:
37°C, pH 6.5, wild-type enzyme
wild-type enzyme


organism: Geobacillus stearothermophilus


proof_lines: mutant enzyme Q160L, in 50 mM MOPS-NaOH, pH 6.0, at 60°C


proof_lines: mutant enzyme Q160K, in 50 mM MOPS-NaOH, pH 6.0, at 60°C


proof_lines: wild type enzyme, in 50 mM MOPS-NaOH, pH 6.0, at 60°C


proof_lines: mutant enzyme Q160E, in 50 mM MOPS-NaOH, pH 6.0, at 60°C


proof_lines: mutant enzyme Q160N, in 50 mM MOPS-NaOH, pH 6.0, at 60°C


proof_lines: mutant enzyme Q160H, in 50 mM MOPS-NaOH, pH 6.0, at 60°C


assay_conditions_summary: pH 7.3; 296.15 K; buffer 10 mM potassium phosphate

proof_lines: mutant enzyme R39A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C


proof_lines: mutant enzyme R11A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C


proof_lines:
below, pH 7.3, 23°C, mutant R11A/R39A
pH 7.3, 23°C, mutant R11A/R39A


proof_lines: wild type enzyme, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C


organism: Choristoneura fumiferana

proof_lines: mutant enzyme Y159F, in 25 mM Tris-HCl, pH 7.0, at 37°C


proof_lines: mutant enzyme Y105F, in 25 mM Tris-HCl, pH 7.0, at 37°C


proof_lines: mutant enzyme E169Q, in 25 mM Tris-HCl, pH 7.0, at 37°C


proof_lines: mutant enzyme W216F, in 25 mM Tris-HCl, pH 7.0, at 37°C


enzyme_display_name: homoglutathione synthase

organism: Glycine max

substrate_name: beta-Alanine

enzyme: homoglutathione synthase

substrate: beta-Alanine


substrate_name: ATP

assay_conditions_summary: cofactor ATP

substrate: ATP


substrate_name: glycine

substrate: glycine


proof_lines: wild type enzyme, in 25 mM Tris-HCl, pH 7.0, at 37°C


organism: Clavibacter michiganensis

substrate_name: gypenoside XVII

substrate: gypenoside XVII


proof_lines: pH 7.5, 35°C, recombinant wild-type enzyme


substrate_name: ginsenoside Rb1

substrate: ginsenoside Rb1


enzyme_display_name: thymidine-triphosphatase

organism: Escherichia phage T7

substrate_name: dTTP

proof_lines: mutant enzyme R493A/N468A, in the presence of M13 single-stranded DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C

proof_lines: mutant enzyme R493A/N468A, in the absence of DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C

enzyme: thymidine-triphosphatase

substrate: dTTP


proof_lines: mutant enzyme N468A, in the absence of DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C

proof_lines: mutant enzyme N468A, in the presence of M13 single-stranded DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C


proof_lines: mutant enzyme R493Q, in the presence of M13 single-stranded DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C

proof_lines: mutant enzyme R493Q, in the absence of DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C


proof_lines: wild type enzyme, in the presence of M13 single-stranded DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C

proof_lines: wild type enzyme, in the absence of DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C


organism: Pseudomonas putida


substrate_name: D-galactose

proof_lines:
recombinant mutant H18T, pH 6.0, 60°C
recombinant mutant H18T, pH 7.5, 60°C

substrate: D-galactose


proof_lines: recombinant mutant H18T/Y234C, pH 6.0, 60°C

proof_lines: recombinant mutant H18T/Y234C, pH 7.5, 60°C


organism: Halalkalibacterium halodurans

substrate_name: L-arabinose

proof_lines:
wild-type at pH 6 and 50°C
wild-type at pH 7 and 50°C
wild-type at pH 8 and 50°C

substrate: L-arabinose


proof_lines:
mutant E268K at pH 6 and 50°C
mutant E268K at pH 7 and 50°C
mutant E268K at pH 8 and 50°C


assay_conditions_summary: pH 7.3; 295.15 K; buffer 10 mM potassium phosphate

proof_lines: mutant enzyme P1A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C


proof_lines: mutant enzyme R61A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C


proof_lines: wild type enzyme, at aerobic conditions, at pH 7.0 and 37°C

proof_lines: wild type enzyme, at anaerobic conditions, at pH 7.0 and 37°C


proof_lines: mutant enzyme N37A, at aerobic conditions, at pH 7.0 and 37°C


enzyme_display_name: iodide peroxidase

organism: Ascophyllum nodosum

substrate_name: I-

assay_conditions_summary: pH 8; 293.15 K; buffer phosphate

proof_lines: in phosphate buffer (100 mM, pH 8.0), at 20°C

enzyme: iodide peroxidase

substrate: I-


organism: Homo sapiens

substrate_name: benzidine

substrate: benzidine


organism: Sus scrofa


assay_conditions_summary: pH 7; 298.15 K; cofactor FAD

proof_lines:
FAD cofactor, pH 7, 25°C
native enzyme, pH 7, 25°C
2’-deoxy-FAD cofactor, pH 7, 25°C


enzyme_display_name: protein-disulfide reductase

organism: Lathyrus oleraceus

substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

enzyme: protein-disulfide reductase

substrate: NADPH


organism: Mycobacterium tuberculosis

substrate_name: NADH

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

proof_lines: pH 7.5, 25°C, with 2,6-dimethyl-1,4-benzoquinone

proof_lines: pH 7.5, 25°C, with 5-hydroxy-1,4-naphthoquinone

substrate: NADH


organism: Priestia megaterium

substrate_name: pantethine 4',4''-diphosphate

assay_conditions_summary: cofactor CoA

substrate: pantethine 4',4''-diphosphate


organism: Escherichia coli

substrate_name: methyl deficient tRNA from E. coli

substrate: methyl deficient tRNA from E. coli


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: undermethylated tRNA

substrate: undermethylated tRNA


organism: denitrifying bacterium

substrate_name: 4-Hydroxybenzyl alcohol

substrate: 4-Hydroxybenzyl alcohol


organism: Geobacter metallireducens

proof_lines:
competitive inhibition
uncompetitive inhibition


substrate_name: UDPglucuronate

substrate: UDPglucuronate


substrate_name: UDP-glucuronate

substrate: UDP-glucuronate


organism: Mus musculus


enzyme_display_name: mogroside IE synthase

organism: Siraitia grosvenorii

substrate_name: quercetin

enzyme: mogroside IE synthase

substrate: quercetin


enzyme_display_name: aminolevulinate transaminase

organism: Bos taurus

proof_lines: pH 7.0, 37°C, 4,5-dioxopentanoate as amino acceptor

proof_lines: pH 7.0, 37°C, glyoxylate as amino acceptor

enzyme: aminolevulinate transaminase


organism: Candida albicans

substrate_name: L-alanine

substrate: L-alanine


organism: Cenchrus americanus


organism: Cereibacter sphaeroides


organism: Leishmania donovani


organism: Raphanus sativus


organism: Rattus norvegicus

proof_lines: different concentrations of alanine at fixed 4,5-dioxopentanoate concentration


organism: Chlorella regularis

substrate_name: glyoxylate

proof_lines: pH 8.0, 37°C, glyoxylate as amino acceptor

substrate: glyoxylate


organism: Clostridium tetanomorphum


organism: Devosia riboflavina


organism: Gallus gallus

assay_conditions_summary: pH 7


organism: Brevibacterium sp.

substrate_name: ADP

assay_conditions_summary: pH 8.4; cofactor ADP

substrate: ADP


organism: Cucumis sativus

substrate_name: UDP

substrate: UDP


organism: Escherichia coli

proof_lines: pH 8.0, 37°C, phosphorolysis of poly(A)tail from rabbit globin mRNA


organism: Kitasatospora aureofaciens

assay_conditions_summary: pH 9.5; 310.15 K; cofactor ADP

proof_lines: approx. value, also for CDP and UDP polymerization


substrate_name: 8-mer 2' phosphorylated RNA

proof_lines:
37°C
pUpU-(2’-phosphate)pU, 37°C
pApA-(2’-phosphate)pA, 37°C

substrate: 8-mer 2' phosphorylated RNA


organism: Saccharomyces cerevisiae

substrate_name: 3-mer 2' phosphorylated RNA

substrate: 3-mer 2' phosphorylated RNA


organism: Micrococcus luteus

substrate_name: heptauridylate

assay_conditions_summary: pH 9; 310.15 K; cofactor ADP

proof_lines: pH 9.0, 37°C, inhibition of ADP polymerization

substrate: heptauridylate


organism: Rhodospirillum rubrum

substrate_name: phosphate

assay_conditions_summary: pH 8; 293.15 K; cofactor ADP

proof_lines: pH 8.0, 20°C, ADP-phosphate-exchange reaction

proof_lines: pH 8.0, 20°C, ADP-phosphate exchange reaction

substrate: phosphate


organism: Sinorhizobium meliloti

substrate_name: Poly(A)

substrate: Poly(A)


organism: Thermus thermophilus

substrate_name: Polyadenylic acid

proof_lines: synthesis of a copolymer of adenylic acid

proof_lines: synthesis of a copolymer of uridylic acid

proof_lines: synthesis of a copolymer of guanylic acid

substrate: Polyadenylic acid


enzyme_display_name: chondroitin 6-sulfotransferase

organism: Mus musculus

substrate_name: native chondroitin

enzyme: chondroitin 6-sulfotransferase

substrate: native chondroitin


organism: Cavia porcellus

substrate_name: n-Decanoyl salicylate

substrate: n-Decanoyl salicylate


organism: Homo sapiens

substrate_name: Acetylsalicylic acid

proof_lines: recombinant catalytic subuit PAFAH1B2, pH 7.2, 37°C

substrate: Acetylsalicylic acid


organism: Bos taurus

substrate_name: decasaccharide

substrate: decasaccharide


organism: Coturnix japonica

substrate_name: chondroitin

proof_lines: 1-phospho-N-acetylgalactosamine 4-sulfate, pH 7, 37°C

proof_lines: + UDP-N-acetylgalactosamine 4-sulfate, pH 7, 37°C

substrate: chondroitin


proof_lines: in the presence of protamine, pH 8, 37°C


substrate_name: chondroitin 4-sulfate

substrate: chondroitin 4-sulfate


enzyme_display_name: poly(ADP-ribose) glycohydrolase

substrate_name: Poly(ADP-ribose)

assay_conditions_summary: 310.15 K; cofactor ADP

enzyme: poly(ADP-ribose) glycohydrolase

substrate: Poly(ADP-ribose)


substrate_name: (ADP-ribose)n

assay_conditions_summary: cofactor ADP

proof_lines: average chain length n = 21, poly(ADP-ribose) glycohydrolase II

proof_lines: average chain length n = 15, poly(ADP-ribose) glycohydrolase I

substrate: (ADP-ribose)n


organism: Sus scrofa


enzyme_display_name: beta-galactofuranosidase

organism: Aspergillus niger

substrate_name: p-nitrophenyl beta-D-galactofuranoside

enzyme: beta-galactofuranosidase

substrate: p-nitrophenyl beta-D-galactofuranoside


organism: Penicillium fellutanum

substrate_name: peptidophosphogalactomannan pP2GM

assay_conditions_summary: pH 4; 313.15 K; buffer 66 mM NaOAc

proof_lines:
40°C, pH 4
in 66 mM NaOAc buffer (pH 4.9), at 37°C
pH 4.6, 37°C

substrate: peptidophosphogalactomannan pP2GM


organism: Streptomyces sp. JHA 19

substrate_name: 4-nitrophenyl beta-D-galactofuranoside

substrate: 4-nitrophenyl beta-D-galactofuranoside


enzyme_display_name: ginsenosidase type III

organism: Panax notoginseng

substrate_name: ginsenoside Rb3

enzyme: ginsenosidase type III

substrate: ginsenoside Rb3


enzyme_display_name: ginsenoside Rb1 beta-glucosidase

organism: Gordonia terrae

substrate_name: ginsenoside Rd

proof_lines:
recombinant enzyme, pH 6.5, 30°C
at pH 6.5 and 30Â°C

enzyme: ginsenoside Rb1 beta-glucosidase

substrate: ginsenoside Rd


organism: Mucilaginibacter sp.

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Paecilomyces sp.

substrate_name: 4-nitrophenyl beta-D-glucoside

substrate: 4-nitrophenyl beta-D-glucoside


organism: Thermoclostridium stercorarium


enzyme_display_name: leucyl endopeptidase

organism: Bacillus cereus

enzyme: leucyl endopeptidase


organism: Spinacia oleracea


organism: Thermotoga petrophila

proof_lines:
isoform bgl1, at pH 6.0 and 90°C
isoform blg1, at pH 6.0 and 90°C


enzyme_display_name: kexin

organism: Mus musculus

substrate_name: Unknown

enzyme: kexin

substrate: Unknown


organism: Saccharomyces cerevisiae

proof_lines:
pH 7.0, 21°C
pH 7.0, 37°C
pH 7.0, 37°C, in natural abundance H2O


enzyme_display_name: arginine deiminase

organism: Pseudomonas plecoglossicida

substrate_name: L-arginine

assay_conditions_summary: pH 7.4; 310.15 K; buffer 0.5 M phosphate

proof_lines: mutant enzyme H404R, in 0.5 M phosphate buffer, pH 7.4, at 37°C

enzyme: arginine deiminase

substrate: L-arginine


enzyme_display_name: proprotein convertase 2

enzyme: proprotein convertase 2


organism: Bacillus subtilis

substrate_name: dTTP

substrate: dTTP


organism: Homo sapiens

proof_lines: competitive inhibitor with dTTP as substrate


enzyme_display_name: 4-hydroxyproline epimerase

organism: Pseudomonas aeruginosa

proof_lines: in 50 mM HEPES, pH 8.0, with 2 mM beta-mercaptoethanol,1 mM EDTA, at 37°C

enzyme: 4-hydroxyproline epimerase


organism: Pseudomonas putida

proof_lines:
solvent D2O
solvent H2O
2-2H-4-hydroxy-L-Pro


organism: Sinorhizobium meliloti

proof_lines: pH not specified in the publication, 30°C, recombinant enzyme


enzyme_display_name: L-arabinose isomerase

organism: Halalkalibacterium halodurans

substrate_name: D-galactose

enzyme: L-arabinose isomerase

substrate: D-galactose


organism: Heyndrickxia coagulans

substrate_name: L-arabinose

substrate: L-arabinose


organism: Lactiplantibacillus plantarum

substrate_name: L-ribulose

substrate: L-ribulose


organism: Lactobacillus gayonii


organism: Bacillus licheniformis

assay_conditions_summary: pH 7.5; 323.15 K; buffer 50 mM phosphate

proof_lines: pH 7.5, 50°C, immobilized recombinant enzyme

proof_lines: immobilized enzyme, in 50 mM phosphate buffer (pH 7.5),1 mM MnCl2, at 50°C


organism: Enterococcus faecium

proof_lines:
at pH 7.0 and 40°C
at pH 7.0 and 30°C
at pH 7.0 and 50°C


organism: Escherichia coli

substrate_name: D-tagatose

proof_lines:
pH 7.0, 37°C, whole cells
pH 7.0, 37°C, purified enzyme

substrate: D-tagatose


organism: Geobacillus stearothermophilus

proof_lines: in the presence of 0.4 mM Mn2+, at pH 7.5 and 60°C

proof_lines: in the presence of 0.2 mM Mn2+, at pH 7.5 and 60°C


organism: Latilactobacillus sakei

assay_conditions_summary: pH 5; buffer 100 mM sodium acetate

proof_lines: with 0.8 mM MgCl2 and 0.8 mM MnCl2, in 100 mM sodium acetate buffer (pH 5.0), at 35°C


organism: Mycolicibacterium smegmatis

proof_lines:
D-galactose induced enzyme
L-arabinose induced enzyme


organism: Pseudoalteromonas haloplanktis

proof_lines:
pH 8.0, 40Â°C, recombinant enzyme
pH 7.5, 75Â°C, recombinant enzyme


organism: Streptomyces sp.


organism: Thermotoga maritima

proof_lines: in the presence of 0.6 mM Mn2+, at pH 7.5 and 60°C

proof_lines: in the presence of 0.8 mM Mn2+, at pH 7.5 and 60°C


organism: Thermotoga neapolitana


enzyme_display_name: 2-hydroxymuconate tautomerase

organism: Helicobacter pylori

substrate_name: phenylenolpyruvate

proof_lines: pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate

enzyme: 2-hydroxymuconate tautomerase

substrate: phenylenolpyruvate


organism: Pseudomonas putida

proof_lines:
pH 7.3, 30°C
at pH 7.3 and 22°C
L-4OT enantiomer, pH 7.3, 30°C


enzyme_display_name: isopentenyl-diphosphate Delta-isomerase

organism: Aedes aegypti

substrate_name: isopentenyl diphosphate

proof_lines: in 0.4 M Tris, 1 mM dithiothreitol, 10 mM MgCl2, pH 8.0, at 50°C

enzyme: isopentenyl-diphosphate Delta-isomerase

substrate: isopentenyl diphosphate


organism: Bombyx mori


organism: Capsicum annuum


organism: Claviceps sp.


organism: Methanothermobacter thermautotrophicus


organism: Narcissus pseudonarcissus


organism: Saccharolobus shibatae

assay_conditions_summary: pH 6


organism: Saccharomyces cerevisiae


organism: Escherichia coli

proof_lines: in 50 mM HEPES (pH 7.2) containing 10 mM MgCl2, 200 mM KCl, 1 mg/ml bovine serum albumin, 0.5 mM dithiothreitol, at 37°C


organism: Gallus gallus

proof_lines:
isomerase IV
isomerase I
isomerase II and III


organism: Homo sapiens

assay_conditions_summary: pH 8


organism: Methanocaldococcus jannaschii


organism: Solanum lycopersicum


organism: Staphylococcus aureus

assay_conditions_summary: pH 7; 310.15 K; cofactor NADPH

proof_lines: aerobic, presence of dithionite, pH 7.0, 37°C

proof_lines: anaerobic, presence of NADPH, pH 7.0, 37°C


organism: Sus scrofa


organism: Thermococcus kodakarensis


organism: Thermus thermophilus

proof_lines: in 200 mM HEPES (pH 7.0) containing 2 mM MgCl2, 0.04 mM FMN, 2 mM NADPH, at 37°C


enzyme_display_name: tryptase

proof_lines:
pH 7.6, 20°C, isoform L1
pH 6.0, 25°C, 200 ng protamine
pH 7.6, 20°C, isoform L2

enzyme: tryptase


enzyme_display_name: homoglutathione synthase

organism: Lathyrus oleraceus

substrate_name: beta-Alanine

enzyme: homoglutathione synthase

substrate: beta-Alanine


organism: Phaseolus coccineus

substrate_name: gamma-glutamylcysteine

substrate: gamma-glutamylcysteine


organism: Vigna radiata var. radiata

substrate_name: ATP

assay_conditions_summary: cofactor ATP

substrate: ATP


organism: Trichomonas vaginalis

substrate_name: H2O; L-Arginine

proof_lines:
strain MR-5
strain MR-100
at pH 6.0 and 37°C

substrate: H2O; L-Arginine


organism: Cinchona robusta

substrate_name: Isopentenyl diphosphate

proof_lines:
isomerase II
isomerase I

substrate: Isopentenyl diphosphate


organism: Mycoplasma arthritidis


enzyme_display_name: aspartate racemase

organism: Enterococcus hirae

substrate_name: L-Aspartate

enzyme: aspartate racemase

substrate: L-Aspartate


enzyme_display_name: cellulase

organism: Pyrococcus horikoshii

substrate_name: p-nitrophenyl cellobiose

enzyme: cellulase

substrate: p-nitrophenyl cellobiose


proof_lines: pH 5-6, 50°C, mutant enzyme lacking 5 residues at the C-terminus and 5 residies at the N-terminus


substrate_name: 4-nitrophenyl cellobioside

proof_lines:
pH 6.0, 70°C
pH 5.5, 50°C
Raw SKiD Env: Temp=50°C, pH=5.5

substrate: 4-nitrophenyl cellobioside


organism: Rasamsonia emersonii

substrate_name: 4-methylumbelliferyl beta-D-lactoside

substrate: 4-methylumbelliferyl beta-D-lactoside


organism: Pseudomonas syringae


enzyme_display_name: steroid C-25 hydroxylase

organism: Sterolibacterium denitrificans

proof_lines: pH 7.5, 30°C, purified enzyme, immobilized enzyme

enzyme: steroid C-25 hydroxylase


organism: Nicotiana attenuata

substrate_name: caffeoyl-CoA

assay_conditions_summary: pH 7.5; 303.15 K; cofactor CoA

proof_lines: with putrescine, recombinant His-tagged enzyme, pH 7.5, 30°C

proof_lines: with caffeoyl-CoA, recombinant His-tagged enzyme, pH 7.5, 30°C

substrate: caffeoyl-CoA


organism: Oryza sativa Japonica Group

substrate_name: feruloyl-CoA

proof_lines: cosubstrate 4-coumaroyl-CoA, pH 7.5, 30°C

substrate: feruloyl-CoA


organism: Solanum lycopersicum

assay_conditions_summary: pH 9; 308.15 K; cofactor CoA


substrate_name: putrescine

substrate: putrescine


organism: Bacillus subtilis

substrate_name: H2O; 4-Nitrophenyl-beta-cellobioside

substrate: H2O; 4-Nitrophenyl-beta-cellobioside


organism: Cellulomonas fimi


organism: Pyrococcus furiosus


substrate_name: 4-nitrophenyl cellotetraoside

substrate: 4-nitrophenyl cellotetraoside


substrate_name: 4-nitrophenyl cellotrioside

substrate: 4-nitrophenyl cellotrioside


substrate_name: 4-nitrophenyl cellopentaoside

substrate: 4-nitrophenyl cellopentaoside


proof_lines: 50°C, mutant enzyme C106A/C159A/C372A/C412A


organism: Thermobifida fusca

substrate_name: 2,4-dinitrophenyl beta-D-cellobioside

substrate: 2,4-dinitrophenyl beta-D-cellobioside


organism: Thermotoga maritima

proof_lines: pH 3, 80°C, chimeric fusion protein CelA-SSO1949-CelA, that consists of 70 amino acids CelA followed by 163 amino acids SSO1949 and 29 amino acids CelA


organism: Trichoderma reesei

substrate_name: 4-methylumbelliferyl cellobioside

substrate: 4-methylumbelliferyl cellobioside


substrate_name: 4-nitrophenyl D-cellobioside

substrate: 4-nitrophenyl D-cellobioside


organism: Homo sapiens

substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


organism: Bacillus sp.

substrate_name: H2O; Carboxymethylcellulose

substrate: H2O; Carboxymethylcellulose


substrate_name: p-Nitrophenyl-beta-D-cellotrioside

substrate: p-Nitrophenyl-beta-D-cellotrioside


substrate_name: p-Nitrophenyl-beta-D-cellotrioside; H2O

substrate: p-Nitrophenyl-beta-D-cellotrioside; H2O


enzyme_display_name: leukotriene-C4 synthase

substrate_name: leukotriene A4

proof_lines:
human recombinant enzyme
IC50 value

enzyme: leukotriene-C4 synthase

substrate: leukotriene A4


proof_lines: mutant R31A, 22°C, pH not specified in the publication


proof_lines: mutant R31Q, 22°C, pH not specified in the publication


proof_lines: mutant R104S, 22°C, pH not specified in the publication


substrate_name: glutathione

substrate: glutathione


substrate_name: leukotriene A4 methyl ester

substrate: leukotriene A4 methyl ester


proof_lines: mutant R104A, 22°C, pH not specified in the publication


proof_lines: wild-type, 22°C, pH not specified in the publication


proof_lines: pH 7.8, temperature not specified in the publication, recombinant mutant W116A


organism: uncultured bacterium

substrate_name: cellotriose

substrate: cellotriose


substrate_name: carboxymethyl cellulose

substrate: carboxymethyl cellulose


substrate_name: cellopentaose

substrate: cellopentaose


substrate_name: Laminarin

substrate: Laminarin


substrate_name: cellohexaose

substrate: cellohexaose


substrate_name: hydroxyethylcellulose

substrate: hydroxyethylcellulose


substrate_name: cellotetraose

substrate: cellotetraose


enzyme_display_name: pepsin B

organism: Canis lupus familiaris

substrate_name: KPAEFFRL

enzyme: pepsin B

substrate: KPAEFFRL


substrate_name: KPAGFARL

substrate: KPAGFARL


substrate_name: KPAEFARL

substrate: KPAEFARL


organism: Pyrococcus horikoshii

substrate_name: L-Aspartate

substrate: L-Aspartate


organism: Thermococcus litoralis


organism: Escherichia coli

substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


organism: Methanococcus maripaludis


organism: Neisseria meningitidis serogroup A / serotype 4A

substrate_name: UDP-N-acetyl-alpha-D-glucosamine

substrate: UDP-N-acetyl-alpha-D-glucosamine


substrate_name: UDP-N-acetyl-alpha-D-mannosamine

proof_lines:
pH 8.5, 37°C, recombinant enzyme
Raw SKiD Env: Temp=37°C, pH=8.5

substrate: UDP-N-acetyl-alpha-D-mannosamine


organism: Rattus norvegicus

substrate_name: UDP-N-acetyl-D-glucosamine; H2O

substrate: UDP-N-acetyl-D-glucosamine; H2O


substrate_name: leukotriene A4

proof_lines: pH 7.8, temperature not specified in the publication, recombinant mutant W116F

substrate: leukotriene A4


substrate_name: glutathione

substrate: glutathione


substrate_name: Leukotriene A4; Reduced glutathione

substrate: Leukotriene A4; Reduced glutathione


substrate_name: Reduced glutathione; Leukotriene A4

substrate: Reduced glutathione; Leukotriene A4


organism: Mus musculus

proof_lines: pH 7.8, temperature not specified in the publication, recombinant enzyme


substrate_name: leukotriene A4 methyl ester

substrate: leukotriene A4 methyl ester


organism: Acropora millepora


organism: Crassostrea gigas


organism: Penaeus monodon


enzyme_display_name: mannose-6-phosphate isomerase

organism: Thermoplasma acidophilum

substrate_name: D-Mannose 6-phosphate

enzyme: mannose-6-phosphate isomerase

substrate: D-Mannose 6-phosphate


organism: Thermus thermophilus

substrate_name: D-mannose 6-phosphate

substrate: D-mannose 6-phosphate


substrate_name: D-fructose 6-phosphate

substrate: D-fructose 6-phosphate


enzyme_display_name: dethiobiotin synthase

substrate_name: (7R,8S)-7,8-diaminopelargonic acid

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

enzyme: dethiobiotin synthase

substrate: (7R,8S)-7,8-diaminopelargonic acid


substrate_name: GTP

substrate: GTP


substrate_name: H2O; UDP-N-acetyl-D-mannosamine

substrate: H2O; UDP-N-acetyl-D-mannosamine


organism: Aeropyrum pernix


organism: Arabidopsis thaliana


organism: Bacillus subtilis

substrate_name: D-ribose

substrate: D-ribose


organism: Burkholderia cepacia

proof_lines: pH 7.6, 5 mM Mg2+, temperature not specified in the publication


organism: Candida albicans

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Chlorella variabilis


organism: Geobacillus thermodenitrificans

proof_lines: mutant enzyme W17Q/N90A/L129F, at pH 7.0 and 70°C


proof_lines: mutant enzyme N90A/L129F, at pH 7.0 and 70°C


organism: Lactobacillus casei


organism: Oryza sativa Japonica Group


organism: Pseudomonas aeruginosa


organism: Pyrobaculum aerophilum


organism: Pyrococcus horikoshii

substrate_name: beta-D-mannose 6-phosphate

substrate: beta-D-mannose 6-phosphate


organism: Rattus norvegicus


organism: Saccharomyces cerevisiae


organism: Sterolibacterium denitrificans

proof_lines:
isoenzyme S25DH4, pH 7.0, 30°C
isoenzyme S25DH3, pH 7.0, 30°C
isoenzyme S25DH2, pH 7.0, 30°C


organism: Escherichia coli

substrate_name: ATP; CO2; 7,8-Diaminononanoate

substrate: ATP; CO2; 7,8-Diaminononanoate


organism: Mycobacterium tuberculosis


substrate_name: CTP

substrate: CTP


substrate_name: TTP

substrate: TTP


organism: Thermus thermophilus

substrate_name: L-ribulose

substrate: L-ribulose


substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


substrate_name: vitamin D3

substrate: vitamin D3


substrate_name: mannose 6-phosphate

assay_conditions_summary: buffer selenomethionine-labelled enzyme Hepes buffer Tris/HCl buffer selenomethionine-labelled enzyme

substrate: mannose 6-phosphate


organism: Nicotiana tabacum

substrate_name: feruloyl-CoA

assay_conditions_summary: pH 10; cofactor CoA

proof_lines:
with caffeoyl-CoA
with 0.25 mM caffeoyl-CoA
pH 10.0

substrate: feruloyl-CoA


substrate_name: asialo-alpha1-acid glycoprotein

proof_lines:
PC-12D cells
PC-12 and PC-12D cells
PC-12 cells

substrate: asialo-alpha1-acid glycoprotein


substrate_name: 2-Aminoethylarsonic acid

substrate: 2-Aminoethylarsonic acid


organism: Bos taurus

substrate_name: lactose

substrate: lactose


organism: Helicobacter pylori

assay_conditions_summary: pH 7.5; 310.15 K; buffer cacodylate

proof_lines: using Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 as acceptor, in cacodylate buffer (100 mM, pH 7.5), at 37°C

proof_lines: using Galbeta(1->3)GlcNAcbeta-O(CH2)3N3 as acceptor, in cacodylate buffer (100 mM, pH 7.5), at 37°C

proof_lines: using Galbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H as acceptor, in cacodylate buffer (100 mM, pH 7.5), at 37°C


organism: Homo sapiens

proof_lines:
lactosylceramide as acceptor
lactose as acceptor
recombinant enzyme, pH 7.2, 37°C


organism: Mus musculus

substrate_name: neolactotetraosylceramide

proof_lines:
membrane-bound enzyme
solubilized enzyme

substrate: neolactotetraosylceramide


enzyme_display_name: oligonucleotidase

proof_lines:
in the presence of 3 mM Mn2+
in the presence of 1 mM Mn2+

enzyme: oligonucleotidase


organism: Flammulina velutipes


organism: Mycolicibacterium smegmatis


substrate_name: p-nitrophenyl uridine 5'-monophosphate

substrate: p-nitrophenyl uridine 5'-monophosphate


organism: Vibrio cholerae


enzyme_display_name: lactase

organism: Sus scrofa

enzyme: lactase


organism: Cavia porcellus

substrate_name: leukotriene A4 methyl ester

substrate: leukotriene A4 methyl ester


substrate_name: glutathione

substrate: glutathione


substrate_name: leukotriene A4

substrate: leukotriene A4


substrate_name: phlorizin

proof_lines:
LPH from uninfected cells
PPH from RRV-infected cells

substrate: phlorizin


organism: Ovis aries


organism: Platyrrhini


organism: Rattus norvegicus

assay_conditions_summary: pH 6; 298.15 K; buffer assay notes

proof_lines: 100 mM sodium maleate buffer, pH 6.0, 25°C

proof_lines: 100 mM sodium malaete buffer, pH 6.0, 25°C


organism: Albatrossia pectoralis

substrate_name: hemoglobin

substrate: hemoglobin


organism: Boreogadus saida


organism: Bacillus cereus

substrate_name: UDP-N-acetyl-alpha-D-mannosamine

proof_lines: in presence of 0.5 mM UDP-N-acetylglucosamine

substrate: UDP-N-acetyl-alpha-D-mannosamine


organism: Escherichia coli

proof_lines: in presence of 1 mM UDP-N-acetylglucosamine


proof_lines:
37°C, pH 7.5
tumor enzyme
and a second Km-value of 0.08 mM


organism: Cassia coluteoides

proof_lines:
enzyme from germinating seeds
enzyme from developing seeds


organism: Dehalococcoides mccartyi

substrate_name: D-mannose 6-phosphate

substrate: D-mannose 6-phosphate


substrate_name: beta-D-mannose 6-phosphate

assay_conditions_summary: pH 7.1; 298.15 K; buffer 50 mM HEPES

proof_lines:
in 50 mM HEPES buffer, pH 7.1, at 25°C
22°C, pH 7.4
pH 7.1, 25°C, recombinant enzyme

substrate: beta-D-mannose 6-phosphate


proof_lines:
in 50 mM HEPES buffer, pH 7.1, at 25°C
recombinant enzyme


organism: Amorphophallus konjac


organism: Arabidopsis thaliana


organism: Bacillus amyloliquefaciens


organism: Burkholderia cenocepacia


organism: Xanthomonas campestris


organism: Homo sapiens


organism: Pseudomonas aeruginosa

proof_lines: bifunctional enzyme: phosphomannose isomerase-guanosine 5-diphospho-D-mannose pyrophosphorylase


organism: Pyrobaculum aerophilum

substrate_name: D-fructose 6-phosphate

substrate: D-fructose 6-phosphate


organism: Saccharomyces cerevisiae

proof_lines:
pH 7.1, 25°C
pH 7.1, 25°C, recombinant enzyme


substrate_name: Reduced glutathione; Leukotriene A4

substrate: Reduced glutathione; Leukotriene A4


organism: Neisseria meningitidis

substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


assay_conditions_summary: pH 7.8; 298.15 K; cofactor ATP


organism: Lysinibacillus sphaericus


organism: Mycobacterium tuberculosis

proof_lines: pH 7.5, temperature not specified in the publication


organism: Lactobacillus plantarum

substrate_name: L-Arabinose

substrate: L-Arabinose


enzyme_display_name: nitrogenase

organism: Unknown

enzyme: nitrogenase


enzyme_display_name: fruit bromelain

substrate_name: Nalpha-benzoyl-Arg amide

enzyme: fruit bromelain

substrate: Nalpha-benzoyl-Arg amide


enzyme_display_name: tubulin GTPase

substrate_name: GTP

enzyme: tubulin GTPase

substrate: GTP


substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication, versus UDP-N-acetyl-D-glucosamine

proof_lines: pH and temperature not specified in the publication, versus GnGnbi-PA

substrate: Unknown


enzyme_display_name: FAD:protein FMN transferase

organism: Vibrio cholerae

substrate_name: FAD

assay_conditions_summary: pH 9; cofactor FAD

proof_lines: pH 9.0, temperature not specified in the publication, mutant H257G

enzyme: FAD:protein FMN transferase

substrate: FAD


proof_lines: pH 9.0, temperature not specified in the publication, mutant H257K


assay_conditions_summary: pH 9

proof_lines: pH 9.0, temperature not specified in the publication, mutant H257E


proof_lines: pH 9.0, temperature not specified in the publication, mutant H257t


proof_lines: pH 9.0, temperature not specified in the publication, wild-type enzyme

proof_lines: above, pH 9.0, 22°C, recombinant wild-type enzyme


enzyme_display_name: lipoprotein lipase

substrate_name: triacylglycerol

proof_lines: from chylomicrons, pH 8.0, 37°C, wild-type enzyme

enzyme: lipoprotein lipase

substrate: triacylglycerol


proof_lines: from chylomicrons, pH 8.0, 37°C, mutant LPLS447X


enzyme_display_name: mannosyl-oligosaccharide glucosidase

organism: Aspergillus brasiliensis

proof_lines: in 20 mM sodium phosphate, pH 7.0, at 25°C

enzyme: mannosyl-oligosaccharide glucosidase


organism: Candida albicans

assay_conditions_summary: pH 7; 310.15 K; buffer pH 7

proof_lines: in 10 mM sodium phosphate buffer pH 7.0 and at 37°C


organism: Ananas comosus


proof_lines:
pH 8.0, 37°C
at pH 5.0 and 37Â°C
at pH 8.0 and 37°C


organism: Azotobacter vinelandii


substrate_name: SCN-

proof_lines: before and after reduction of the nitrogenase complex relatively slow reactions take place, which limits the rate of the Fe protein cycle

substrate: SCN-


organism: Gallus gallus


proof_lines:
Hep3B cells
HepG2 cells


organism: Rattus norvegicus

substrate_name: pyridylaminated acceptor substrate

proof_lines:
recombinant enzyme
with UDP-GlcNAc as donor substrate
with UDP-Glc as donor substrate

substrate: pyridylaminated acceptor substrate


enzyme_display_name: rubber cis-polyprenylcistransferase

organism: Ficus carica

substrate_name: farnesyl diphosphate

enzyme: rubber cis-polyprenylcistransferase

substrate: farnesyl diphosphate


organism: Ficus elastica

substrate_name: isopentenyl diphosphate

substrate: isopentenyl diphosphate


organism: Hevea brasiliensis


organism: Parthenium argentatum


enzyme_display_name: beta-glucoside kinase

organism: Klebsiella aerogenes

substrate_name: arbutin

assay_conditions_summary: pH 7.5; cofactor ATP

enzyme: beta-glucoside kinase

substrate: arbutin


organism: Klebsiella pneumoniae

substrate_name: Salicin

substrate: Salicin


organism: Bos taurus

substrate_name: triolein

proof_lines: 25°C, pH 8.5, hydrolysis of p-nitrophenyl butyrate

substrate: triolein


substrate_name: very-low-density lipoprotein

substrate: very-low-density lipoprotein


organism: Homo sapiens


substrate_name: Very-low-density lipoproteins

proof_lines:
enzyme from post-heparin plasma
soluble enzyme

substrate: Very-low-density lipoproteins


enzyme_display_name: granzyme A

substrate_name: Benzyloxycarbonyl-Lys-thiobenzyl ester

proof_lines:
human granzyme A
pH 7.5, 30°C

enzyme: granzyme A

substrate: Benzyloxycarbonyl-Lys-thiobenzyl ester


organism: Mus musculus

substrate_name: benzyloxycarbonyl-Lys thiobenzyl ester

substrate: benzyloxycarbonyl-Lys thiobenzyl ester


organism: Sus scrofa

proof_lines:
strong inhibition
strong specific inhibition


organism: Vigna radiata


assay_conditions_summary: pH 5

proof_lines:
fruit bromelain A
fruit bromelain B
fruit bromelain C


organism: Bromelia karatas

substrate_name: ovalbumin

substrate: ovalbumin


organism: Bromelia pinguin


proof_lines:
very slight inhibition
strong inhibition


organism: Entamoeba histolytica


organism: Escherichia coli

substrate_name: nigerose

substrate: nigerose


organism: Saccharomyces cerevisiae


substrate_name: GTP

assay_conditions_summary: pH 7

proof_lines: in 0.01 M sodium phosphate and 4 mM MgCl2, pH 7

substrate: GTP


proof_lines: tubulin in complex with T2R, ligand colchicine

proof_lines: tubulin in complex with T2R, ligand podophylotoxin


organism: Prosthecobacter sp.

proof_lines: activity of BtubB when mixed 1:1 with BtubA

proof_lines: activity of BtubB, the activity increases to 0.022 per sec when mixed 1:1 with BtubA


enzyme_display_name: leucoanthocyanidin reductase

organism: Desmodium uncinatum

enzyme: leucoanthocyanidin reductase


organism: Vitis vinifera


enzyme_display_name: (-)-borneol dehydrogenase

organism: Pseudomonas sp. TCU-HL1

substrate_name: (-)-borneol

proof_lines: refolded recombinant protein, pH 7, 25°C

enzyme: (-)-borneol dehydrogenase

substrate: (-)-borneol


enzyme_display_name: catechol O-methyltransferase

organism: Mycobacterium tuberculosis

substrate_name: S-Adenosyl-L-methionine; Caffeate

enzyme: catechol O-methyltransferase

substrate: S-Adenosyl-L-methionine; Caffeate


organism: Rattus norvegicus

substrate_name: S-Adenosyl-L-methionine; L-Adrenaline

proof_lines: enzyme from pooled liver S9, at pH 7.4 and 37°C

substrate: S-Adenosyl-L-methionine; L-Adrenaline


enzyme_display_name: N-terminal methionine N(alpha)-acetyltransferase NatF

organism: Arabidopsis thaliana

substrate_name: acetyl-CoA

assay_conditions_summary: cofactor CoA

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: acetyl-CoA


proof_lines: mutant P47A, pH not specified in the publication, temperature not specified in the publication

enzyme: N-terminal methionine N(alpha)-acetyltransferase NatF


proof_lines: SUMO-tagged wild-type, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant 181A, pH not specified in the publication, temperature not specified in the publication


enzyme_display_name: adenylate cyclase

substrate_name: ATP

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP

proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant K136A, 10 mM Mg2+

enzyme: adenylate cyclase

substrate: ATP


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant K332A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant K253A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain, 10 mM Mg2+


substrate_name: Unknown

proof_lines: pH 8.0, 37°C, versus ATP, wild-type catalytic domain Cya2-446

substrate: Unknown


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant W374A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant S103A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant T189A, 10 mM Mg2+


organism: Euglena gracilis

assay_conditions_summary: cofactor ATP


organism: Haloactinopolyspora alba

assay_conditions_summary: pH 10; 313.15 K; cofactor ATP


organism: Homo sapiens


verification_status: verified

assay_conditions_summary: 303.15 K; cofactor ATP

column_label: TABLE III; row Wild type AC; column KmATP (Mn2+)

normalized_value_unit_evidence: Per-metric normalized values and units are preserved in paper_metric_evidence.

raw_value_unit_evidence: Per-metric raw values and units are preserved in paper_metric_evidence; captured_primary_segment preserves the exact source transcription.

row_label: Wild type AC / Mn2+

table_label: TABLE III - Determination of kinetic parameters for the wild type AC in the presence of Mg2+ and Mn2+

status: verified


proof_lines: mutant F46A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant E97A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant I48A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant F180A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant Y115A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant Y50A, pH not specified in the publication, temperature not specified in the publication


enzyme_display_name: gentamicin 2'-N-acetyltransferase

organism: Mycolicibacterium smegmatis

substrate_name: neomycin

assay_conditions_summary: pH 6.5; 298.15 K; cofactor CoA

enzyme: gentamicin 2'-N-acetyltransferase

substrate: neomycin


substrate_name: sisomicin

substrate: sisomicin


enzyme_display_name: 1,3-alpha-oligoglucan phosphorylase

organism: Lachnoclostridium phytofermentans

substrate_name: kojibiose

proof_lines: pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate

proof_lines: pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate

proof_lines: pH 7.0, 30°C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose

enzyme: 1,3-alpha-oligoglucan phosphorylase

substrate: kojibiose


enzyme_display_name: thioglucosidase

substrate_name: H2O; Sinigrin

enzyme: thioglucosidase

substrate: H2O; Sinigrin


organism: Armoracia rusticana

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

proof_lines:
pH 5.5, 37°C
recombinant enzyme, pH 5.5, 37°C

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Anabaena sp.

assay_conditions_summary: pH 8.5; 318.15 K; cofactor ATP