assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

proof_lines: 37°C, pH 7.5, recombinant cyaB1 AC, in the presence of 2 mM Mn2+ and 0.1 mM cAMP

proof_lines: 37°C, pH 7.5, recombinant cyaB1 AC, in the presence of 10 mM Mg2+ and 0.1 mM cAMP


enzyme_display_name: adenylate cyclase

organism: Dictyostelium discoideum

substrate_name: ATP

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

protein_accession_database: UniProt

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

km_unit: mM

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

assay_conditions_summary: cofactor ATP

enzyme: adenylate cyclase

substrate: ATP


organism: Escherichia coli

source_db: brenda

source_license: CC BY 4.0

mutation_type: substitution

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP

proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant W249A, 10 mM Mg2+

source: brenda


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant Y394A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant E185A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant E242A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant S106A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant R19A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant K260A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant S113A, 10 mM Mg2+


enzyme_display_name: (R,R)-butanediol dehydrogenase

organism: Clostridium autoethanogenum

enzyme: (R,R)-butanediol dehydrogenase


source_db: oed

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

smiles: CCC(C)=O

source: oed


substrate_name: acetone

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

substrate: acetone


substrate_name: NADPH

substrate: NADPH


substrate_name: (R)-acetoin

substrate: (R)-acetoin


organism: Cupriavidus necator

substrate_name: NADH; H+; Acetoin

assay_conditions_summary: cofactor NADPH

substrate: NADH; H+; Acetoin


organism: Mycobacterium sp.


organism: Paenibacillus polymyxa

assay_conditions_summary: pH 8; 295.15 K; cofactor NAD+


organism: Pseudomonas aeruginosa


organism: Rhodococcus erythropolis

substrate_name: diacetyl

assay_conditions_summary: pH 10; 318.15 K; cofactor NAD+

proof_lines: with diacetyl as cosubstrate, at pH 10.0 and 45°C

substrate: diacetyl


organism: Saccharomyces cerevisiae

substrate_name: NAD+; (R,R)-Butane-2,3-diol

assay_conditions_summary: cofactor NAD+

substrate: NAD+; (R,R)-Butane-2,3-diol


substrate_name: Acetoin

smiles: CC(=O)C(C)O

substrate: Acetoin


substrate_name: NADH

substrate: NADH


assay_conditions_summary: cofactor NADH


substrate_name: glycolaldehyde

assay_conditions_summary: pH 7; 303.15 K; cofactor NADH

proof_lines: recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B

proof_lines: recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y

substrate: glycolaldehyde


substrate_name: acetaldehyde

substrate: acetaldehyde


enzyme_display_name: methionine gamma-lyase

organism: Citrobacter freundii

substrate_name: methiin

enzyme: methionine gamma-lyase

substrate: methiin


substrate_name: alliin

substrate: alliin


substrate_name: L-methionine

substrate: L-methionine


substrate_name: S-methyl-L-cysteine

source_db: skid

source_license: CC BY-NC-ND 4.0

substrate: S-methyl-L-cysteine

source: skid


organism: Entamoeba histolytica

substrate_name: H2O; L-Methionine

substrate: H2O; L-Methionine


proof_lines:
36°C, pH 6.8, recombinant EhMGL2
36°C, pH 6.8, recombinant EhMGL1


substrate_name: trifluoromethionine

substrate: trifluoromethionine


organism: Homo sapiens

proof_lines: non-cleavable caspase-8 + 0.001 mM FLIP, 37°C, pH 7.4, in the presence of 0.7 M sodium citrate

proof_lines: cleaved caspase-8, 37°C, pH 7.4, in the presence of 0.7 M sodium citrate


enzyme_display_name: memapsin 1

substrate_name: Unknown

km_display: Not reported

ki_unit: mM

proof_lines: pH and temperature not specified in the publication

proof_lines: pH 6.2, temperature not specified in the publication

proof_lines: pH 4, temperature not specified in the publication

enzyme: memapsin 1

substrate: Unknown


enzyme_display_name: creatinase

organism: Actinobacillus sp.

substrate_name: Creatine

source_license: CC BY 4.0; CC BY-NC-ND 4.0

proof_lines: parameter calculated for the bioelectrode, depends on the electrode material and the enzyme immobilization process, indicates that nanocomposite matrix has a high affinity to immobilize CAH enzyme, the small Km value indicates a high enzyme

source_databases_merged: brenda skid

enzyme: creatinase

substrate: Creatine


organism: Glutamicibacter nicotianae


organism: Pseudomonas putida

mutation_type: deletion

proof_lines: pH 7.7, 50°C, creatinase in yeast spores with OSW2 deletion


proof_lines:
pH 7.7, 50°C, soluble enzyme
Raw SKiD Env: Temp=50°C, pH=7.7


proof_lines: pH 7.7, 50°C, creatinase in wild-type yeast spores


proof_lines: pH 7.7, 50°C, creatinase in yeast spores with DIT1 deletion


proof_lines:
wild-type
wild-type, pH 7.2, 30°C


substrate_name: DL-homocysteine

substrate: DL-homocysteine


substrate_name: L-cysteine

substrate: L-cysteine


organism: Fusobacterium nucleatum subsp. nucleatum

proof_lines:
pH 7.6, 37°C
Raw SKiD Env: Temp=37°C, pH=7.6


organism: Micromonospora echinospora

proof_lines:
pH 8.0, 23°C
Raw SKiD Env: Temp=23°C, pH=8


organism: Porphyromonas gingivalis


enzyme_display_name: guanylate cyclase

organism: Catenaria anguillulae

substrate_name: GTP

proof_lines: pH 7.5, 22°C, wild-type enzyme, mutant enzyme E497K/C566D

proof_lines: pH 7.5, 22°C, wild-type enzyme, wild-type enzyme

enzyme: guanylate cyclase

substrate: GTP


organism: Dictyostelium discoideum


proof_lines: pH and temperature not specified in the publication, mutant V902L enzyme in presence or absence of activator GCAP2

proof_lines: pH and temperature not specified in the publication, mutant V902L enzyme in presence of activator GCAP2

proof_lines: pH and temperature not specified in the publication, mutant V902L enzyme in presence of activator GCAP1


proof_lines: pH and temperature not specified in the publication, wild-type and mutant V902L enzymes in presence of activator GCAP1

proof_lines: pH and temperature not specified in the publication, wild-type enzyme in presence of activator GCAP1


enzyme_display_name: amino-acid racemase

organism: Bacillus subtilis

substrate_name: D-Lysine

enzyme: amino-acid racemase

substrate: D-Lysine


substrate_name: L-Lysine

substrate: L-Lysine


organism: Escherichia coli

substrate_name: L-Homoserine

substrate: L-Homoserine


substrate_name: L-homoserine

substrate: L-homoserine


organism: Latilactobacillus sakei

substrate_name: D-alanine

proof_lines: pH 10.0, 35°C, recombinant His-tagged enzyme

substrate: D-alanine


organism: Pyrococcus horikoshii

substrate_name: L-valine

proof_lines:
pH 7.0, 80°C, recombinant enzyme
pH 7.0, 80°C

substrate: L-valine


substrate_name: L-Alanine

substrate: L-Alanine


proof_lines:
pH 7.5, 37°C
at pH 7.5 and 37°C


proof_lines: pH 8.0, 37°C, recombinant wild-type enzyme


proof_lines: pH 8.0, 37°C, recombinant mutant Y114F/C116H


proof_lines: pH 8.0, 37°C, recombinant mutants K240R and K240M


organism: Thermococcus kodakarensis

substrate_name: D-methionine

substrate: D-methionine


organism: Thermococcus litoralis

substrate_name: D-leucine

substrate: D-leucine


enzyme_display_name: ribulose-phosphate 3-epimerase

organism: Bacillus methanolicus

substrate_name: D-ribulose 5-phosphate

enzyme: ribulose-phosphate 3-epimerase

substrate: D-ribulose 5-phosphate


substrate_name: D-Ribulose 5-phosphate

substrate: D-Ribulose 5-phosphate


organism: Oryza sativa


organism: Solanum tuberosum


organism: Spinacia oleracea


enzyme_display_name: steroid Delta-isomerase

assay_conditions_summary: pH 7

proof_lines: mutant enzyme Y30F/Y55F, at pH 7.0, temperature not specified in the publication

enzyme: steroid Delta-isomerase


proof_lines: mutant enzyme Y55F, at pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme Y30F/Y55F/Y115F, at pH 7.0, temperature not specified in the publication


proof_lines: wild type enzyme, at pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme Y30F, at pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme Y14F, at pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme Y115F, at pH 7.0, temperature not specified in the publication


organism: Sus scrofa

substrate_name: NAD+; 7alpha-Hydroxycholesterol

substrate: NAD+; 7alpha-Hydroxycholesterol


enzyme_display_name: complement factor I

organism: Homo sapiens

proof_lines: mutant R130Q/R169Q, Vmax: 0.16 pmol/min/microgram protein

enzyme: complement factor I


proof_lines: mutant K51A/R62A, Vmax: 0.373 pmol/min/microgram protein


proof_lines: mutant K51A/R62A/L73A/L76A/F82A, Vmax: 0.085 pmol/min/microgram protein


proof_lines: mutant D207N/Q219A, Vmax: 0.044 pmol/min/microgram protein


enzyme_display_name: homospermidine synthase

organism: Unknown

substrate_name: putrescine

enzyme: homospermidine synthase

substrate: putrescine


enzyme_display_name: thioglucosidase

organism: Sinapis alba

substrate_name: H2O; Sinigrin

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

proof_lines:
with dioxane as solvent
with acetonitrile as solvent
with 12.5 M methanol as solvent

enzyme: thioglucosidase

substrate: H2O; Sinigrin


substrate_name: L-ethionine

substrate: L-ethionine


substrate_name: ATP

substrate: ATP


substrate_name: GTP

proof_lines: pH 7.5, 37°C, not stimulated recombinant enzyme

proof_lines: pH 7.5, 37°C, NO-stimulated recombinant enzyme

substrate: GTP


organism: Mus musculus

proof_lines: nonstimulated isozyme RetGC1, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C

proof_lines: nonstimulated isozyme RetGC2, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C

proof_lines: isozyme RetGC1, stimulated by guanylyl cyclase activating protein 2, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C


substrate_name: L-Leu

substrate: L-Leu


proof_lines: mutant K93Q/F94A, Vmax: 0.147 pmol/min/microgram protein


proof_lines: mutant K124Q/R150Q/F151A/K152Q, Vmax: 0.1 pmol/min/microgram protein


proof_lines: mutant L73A/L76A/F82A, Vmax: 0.053 pmol/min/microgram protein


proof_lines: mutant R150Q/F151A/K152Q, Vmax: 0.087 pmol/min/microgram protein


proof_lines: mutant D26N/K27Q/F29A/Q31A, Vmax: 0.834 pmol/min/microgram protein


proof_lines: mutant K182Q/R184Q, Vmax: 0.058 pmol/min/microgram protein


proof_lines: mutant V212A/L236A, Vmax: 2.126 pmol/min/microgram protein


proof_lines: mutant M220A/K221Q, Vmax: 0.01 pmol/min/microgram protein


enzyme_display_name: trimethyllysine dioxygenase

proof_lines: recombinant MBP-tagged enzyme, pH 7.0, 37°C

proof_lines: recombinant detagged enzyme, pH 7.0, 37°C

enzyme: trimethyllysine dioxygenase


organism: Rattus norvegicus


enzyme_display_name: methyl-branched lipid omega-hydroxylase

organism: Mycobacterium tuberculosis

substrate_name: H+; NADPH; O2; 15-Methyl-palmitic acid

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

proof_lines:
at pH 7.4 and 25°C
Raw SKiD Env: Temp=25°C, pH=7.4

source_databases_merged: sabio_rk brenda skid

enzyme: methyl-branched lipid omega-hydroxylase

substrate: H+; NADPH; O2; 15-Methyl-palmitic acid


organism: Bacillus sp. (in: firmicutes)

substrate_name: (R)-acetoin

proof_lines:
at pH 6.0 and 50°C
at pH 9.0 and 50°C

substrate: (R)-acetoin


organism: Bacillus subtilis subsp. subtilis

proof_lines: pH 7.0, 25°C, recombinant His-tagged enzyme


organism: Bacillus thuringiensis serovar kurstaki


organism: Clostridium autoethanogenum

substrate_name: acetaldehyde

smiles: CC=O

substrate: acetaldehyde


substrate_name: acetone

smiles: CC(C)=O

substrate: acetone


enzyme_display_name: glucan 1,4-alpha-maltotetraohydrolase

organism: Roseateles saccharophilus

substrate_name: maltotetraose

enzyme: glucan 1,4-alpha-maltotetraohydrolase

substrate: maltotetraose


proof_lines: non-cleavable caspase-8 + 0.001 mM FLIP D297A, 37°C, pH 7.4, in the presence of 0.7 M sodium citrate


enzyme_display_name: candidapepsin

organism: Candida albicans

substrate_name: Unknown

enzyme: candidapepsin

substrate: Unknown


substrate_name: Lys-Pro-Ala-Arg-Phe-Nph-Arg-Leu

proof_lines:
pH 3.25, isoenzyme Sap2p
isoenzyme Sap2p

substrate: Lys-Pro-Ala-Arg-Phe-Nph-Arg-Leu


organism: Candida parapsilosis

assay_conditions_summary: pH 3.75; 310.15 K; buffer 150 mM sodium citrate

proof_lines: isoform Sapp1p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C

proof_lines: pH not specified in the publication, temperature not specified in the publication


proof_lines: isoform Sapp2p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C


organism: Pseudomonas putida

substrate_name: L-cysteine

proof_lines: pH 8.0, 37°C, recombinant mutants K240I and K240N

substrate: L-cysteine


substrate_name: H2O; L-Methionine

substrate: H2O; L-Methionine


organism: Streptomyces avermitilis

substrate_name: L-homocysteine

substrate: L-homocysteine


organism: Treponema denticola


organism: Trichomonas vaginalis


enzyme_display_name: L-3-cyanoalanine synthase

organism: Anthocharis cardamines

enzyme: L-3-cyanoalanine synthase


substrate_name: hydrogen sulfide

substrate: hydrogen sulfide


organism: Aporia crataegi


organism: Arabidopsis thaliana

substrate_name: L-Cysteine; Cyanide

substrate: L-Cysteine; Cyanide


organism: Colias croceus


organism: Glycine max

proof_lines:
recombinant enzyme, pH 9.0, 25°C
Raw SKiD Env: Temp=25°C, pH=9


organism: Gonepteryx rhamni


enzyme_display_name: type III protein arginine methyltransferase

organism: Homo sapiens

substrate_name: acteyl-KKGGRGRKGKKY-amide

proof_lines: in the absence of KCl, at pH 9.5 and 25°C

proof_lines: in the presence of 130 mM KCl, at pH 9.5 and 25°C

substrate: acteyl-KKGGRGRKGKKY-amide


organism: Mus musculus

substrate_name: [histone H2B peptide 23-37]-L-arginine

proof_lines: with {[heat shock protein A8]-L-arginine469 as cosubstrate, at pH 8.0, temperature not specified in the publication

enzyme: type III protein arginine methyltransferase

substrate: [histone H2B peptide 23-37]-L-arginine


enzyme_display_name: D-alanine 2-hydroxymethyltransferase

organism: Aminobacter sp. AJ110403

substrate_name: tetrahydrofolate

enzyme: D-alanine 2-hydroxymethyltransferase

substrate: tetrahydrofolate


enzyme_display_name: ganglioside galactosyltransferase

organism: Campylobacter jejuni

substrate_name: GalNAcalpha-FCHASE

proof_lines: using fusion construct CgtBOH4384DELTA30-MalE (mutant with deletion of 30 amino acids from carboxy terminus + C-terminal MalE fusion)

enzyme: ganglioside galactosyltransferase

substrate: GalNAcalpha-FCHASE


organism: Bradyrhizobium japonicum

substrate_name: putrescine

substrate: putrescine


organism: Lachancea kluyveri


organism: Roseateles saccharophilus


organism: Pieris rapae


organism: Plutella xylostella


organism: Pseudomonas putida

substrate_name: O-Acetyl-L-serine; Cyanide

substrate: O-Acetyl-L-serine; Cyanide


organism: Solanum tuberosum


organism: Spinacia oleracea


organism: Spodoptera littoralis


organism: Tetranychus urticae

proof_lines: pH 8.5, 37°C, recombinant His-tagged enzyme


organism: Zygaena filipendulae


enzyme_display_name: adenylate cyclase

organism: Plasmodium falciparum

substrate_name: ATP

proof_lines: recombinant AC expressed from synthetic AC gene PfAC526-884

enzyme: adenylate cyclase

substrate: ATP


organism: Rattus norvegicus

proof_lines: sAC activity in cytosolic extracts in the presence of Mn2+


organism: Rhizobium etli


organism: Thermobifida halotolerans

assay_conditions_summary: pH 10; 318.15 K; cofactor ATP


organism: Trypanosoma cruzi


organism: Yersinia pestis

assay_conditions_summary: pH 7; 310.15 K; cofactor ATP


assay_conditions_summary: pH 8.5; 310.15 K; cofactor ATP

proof_lines: mutant enzyme E136A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme E136A, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C


proof_lines: mutant enzyme D55K, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme D55K, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C


organism: Catenaria anguillulae

substrate_name: GTP

substrate: GTP


organism: Homo sapiens

proof_lines:
recombinant sGC
Raw SKiD Env: Temp=-----, pH=-----


proof_lines: mutant T500A, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S502E, temperature not specified in the publication, pH not specified in the publication


proof_lines: wild-type, 37°C, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S473A, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant T500E, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S497A, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant T513A, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S473E, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S502A, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S506E, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S497E, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S510A, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S506A, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S487E, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S510E, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S487A, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant T513E, temperature not specified in the publication, pH not specified in the publication


organism: Ipomoea nil


organism: Leishmania donovani

proof_lines:
recombinant enzyme
purified enzyme


substrate_name: D-ribulose 5-phosphate

substrate: D-ribulose 5-phosphate


enzyme_display_name: protein-glutamate O-methyltransferase

organism: Unknown

substrate_name: S-adenosyl-L-methionine

enzyme: protein-glutamate O-methyltransferase

substrate: S-adenosyl-L-methionine


enzyme_display_name: guanylate cyclase

enzyme: guanylate cyclase


organism: Streptococcus pyogenes


substrate_name: D-Ribulose 5-phosphate

proof_lines: RPE metal-free, stoichiometry of Zn2+: 0.1 ion per polypeptide, substrate 10 mM D-ribulose 5-phosphate

proof_lines: RPE as isolated + 0.5 mM ZnCl2 present in assay, stoichiometry of Zn2+: 0.9 ion per polypeptide, substrate 10 mM D-ribulose 5-phosphate

proof_lines: RPE as isolated, stoichiometry of Zn2+: 0.3 ion per polypeptide, substrate 10 mM D-ribulose 5-phosphate

substrate: D-Ribulose 5-phosphate


enzyme_display_name: aldose 1-epimerase

organism: Escherichia coli

substrate_name: alpha-D-Glucose

enzyme: aldose 1-epimerase

substrate: alpha-D-Glucose


proof_lines:
pH 8.0, 20°C
Raw SKiD Env: Temp=20°C, pH=8


enzyme_display_name: L-ribulose-5-phosphate 4-epimerase

substrate_name: L-Ribulose 5-phosphate

enzyme: L-ribulose-5-phosphate 4-epimerase

substrate: L-Ribulose 5-phosphate


enzyme_display_name: steroid Delta-isomerase

organism: Comamonas testosteroni

enzyme: steroid Delta-isomerase


proof_lines:
mutant D38E, pH 7.0, 29.9 °C
mutant D38E, pH 7.0, 5.4 °C
mutant D38E, pH 7.0, 25.0 °C


proof_lines:
Raw SKiD Env: Temp=25°C, pH=7
Raw SKiD Env: Temp=°C, pH=7


organism: Pseudomonas putida


proof_lines:
25°C, pH 7.0, wild-type enzyme
pH 7.0, 25°C, wild-type enzyme
wild-type, 25°C, pH 7.0


enzyme_display_name: creatinase

substrate_name: Creatine

enzyme: creatinase

substrate: Creatine


enzyme_display_name: complement factor I

proof_lines: mutant D207N/Q219A/M220A/K221Q, Vmax: 0.032 pmol/min/microgram protein

enzyme: complement factor I


proof_lines: mutant F29A/Q31A, Vmax: 0.854 pmol/min/microgram protein


proof_lines: mutant F94A/K182Q/R184Q, Vmax: 0.672 pmol/min/microgram protein


proof_lines: wild-type, Vmax: 0.09 pmol/min/microgram protein


enzyme_display_name: cephaeline 6'-O-methyltransferase

organism: Carapichea ipecacuanha

substrate_name: N-deacetylisoipecoside

source_db: uniprot

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

proof_lines: kcat is 11.3x10(-3) sec(-1) with N-deacetylisoipecoside as substrate (PubMed:20061395). kcat is 20.4x10(-3) sec(-1) with 7-O-methyl-N-deacetylisoipecoside as substrate (PubMed:20061395). kcat is 0.28x10(-3) sec(-1) with cephaeline as substr

enzyme: cephaeline 6'-O-methyltransferase

substrate: N-deacetylisoipecoside

source: uniprot


organism: Aquifex aeolicus


proof_lines: pH 7.5, 55°C, recombinant mutants L60A, F140A, and E113A


proof_lines: pH 7.5, 55°C, recombinant wild-type enzyme


proof_lines:
pH 7.0, 25°C, mutant enzyme D99L
25°C, pH 7.0, mutant enzyme D99L


proof_lines: mutant V252A/I267A, Vmax: 1.284 pmol/min/microgram protein


substrate_name: L-ribulose 5-phosphate

substrate: L-ribulose 5-phosphate


proof_lines: 25°C, pH 7.0, mutant enzyme Y14F/Y30F/Y55F/D99L


proof_lines: 25°C, pH 7.0, mutant enzyme Y14F/Y30F/Y55F


proof_lines: 25°C, pH 7.0, mutant enzyme Y14F/Y30F/Y55F/D99N


organism: Salmonella enterica

proof_lines: pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate, R340K mutant enzyme


proof_lines: pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate, R340A mutant enzyme


proof_lines: pH 8.5, 25°C, synthesis of 2-phosphonoacetaldehyde, R340K and R340A mutant enzymes


substrate_name: D-alanine

proof_lines: pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate

proof_lines: pH 8.5, 25°C, synthesis of 2-phosphonoacetaldehyde

substrate: D-alanine


proof_lines:
wild-type, 27°C, pH 7.4
27°C, pH 7.4, wild-type enzyme
wild-type, isoform 1, 27°C, pH 7.4


organism: Lactococcus lactis

substrate_name: alpha-D-xylose

substrate: alpha-D-xylose


substrate_name: alpha-D-glucose

substrate: alpha-D-glucose


enzyme_display_name: deoxyribodipyrimidine endonucleosidase

organism: Tequatrovirus T4

substrate_name: DNA

enzyme: deoxyribodipyrimidine endonucleosidase

substrate: DNA


enzyme_display_name: flavone apiosyltransferase

organism: Apium graveolens

substrate_name: apigenin 7-O-beta-D-glucoside

enzyme: flavone apiosyltransferase

substrate: apigenin 7-O-beta-D-glucoside


substrate_name: UDP-alpha-D-apiose

substrate: UDP-alpha-D-apiose


enzyme_display_name: (-)-borneol dehydrogenase

substrate_name: (-)-borneol

enzyme: (-)-borneol dehydrogenase

substrate: (-)-borneol


assay_conditions_summary: pH 7.5; 303.15 K; cofactor NAD+


organism: Bordetella pertussis

substrate_name: ATP

assay_conditions_summary: pH 8; 303.15 K; cofactor ATP

proof_lines: mutant enzyme R338A/D360A, at pH 8.0 and 30°C

substrate: ATP


assay_conditions_summary: 303.15 K; cofactor ATP


proof_lines:
wild type isoform VI, 30°C
wild type isoform V, 30°C


proof_lines: 37°C, pH 7.6, Zn2+-form after dialysis + 0.1 mM Zn2+, mutant enzyme H97N or D76N


proof_lines: 37°C, pH 7.6, Zn2+-form after dialysis + 0.1 mM Zn2+, mutant enzyme H95N

proof_lines: 37°C, pH 7.6, Zn2+-form after dialysis, mutant enzyme H95N

proof_lines: 22°C, pH 7.5, mutant enzyme H95N, activated by 0.1 mM ZnCl2


proof_lines: 37°C, pH 7.6, Zn2+-form after dialysis, mutant enzyme D76N

proof_lines: 37°C, pH 7.6, Zn2+-form after dialysis + 0.1 mM Zn2+, mutant enzyme D76N


organism: Mammalia

assay_conditions_summary: pH 7.4; 303.15 K; cofactor ATP

proof_lines: mutant VC1-A409P+IIC2, 30°C, pH 7.4, 10 mM MnCl, 0.1 mM forskolin


proof_lines: mutant VC1+IIC2-I1006V, 30°C, pH 7.4, 10 mM MnCl, 0.1 mM forskolin


proof_lines: mutant VC1-A406P+IIC2-I1006V, 30°C, pH 7.4, 10 mM MnCl, 0.1 mM forskolin


proof_lines: 22°C, pH 7.5, mutant enzyme Y229F, activated by 0.1 mM ZnCl2


proof_lines: 22°C, pH 7.5, wild-type enzyme, activated by 0.1 mM MgCl2

proof_lines: 37°C, pH 7.6, Zn2+-form after dialysis, wild-type


proof_lines: 22°C, pH 7.5, mutant enzyme H97N, activated by 0.1 mM ZnCl2

proof_lines: 37°C, pH 7.6, Zn2+-form after dialysis + 0.1 mM Zn2+, mutant enzyme H97N

proof_lines: 37°C, pH 7.6, Zn2+-form after dialysis, mutant enzyme H97N


substrate_name: dehydroepiandrosterone

substrate: dehydroepiandrosterone


proof_lines:
mutant Q105M, isoform 1, 27°C, pH 7.4
mutant Q105M, isoform 2, 27°C, pH 7.4


proof_lines: mutant T127M, same Km value as the wild-type

proof_lines: wild-type, Km of the mutants 31 - 1100 nM


organism: Aeromonas hydrophila

substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


proof_lines: mutants with recombinant plasmids expression of C- or N-domains


organism: Synechocystis sp.

mutation_signature: truncated Cya1

mutation_type: truncation

assay_conditions_summary: pH 7.5; 300.15 K; cofactor ATP


mutation_signature: truncated Cya1 / 50 mM NaHCO3 truncated Cya1

proof_lines: truncated Cya1, pH 7.5, 27°C, 50 mM NaHCO3


organism: Oryza sativa

substrate_name: L-cysteine

substrate: L-cysteine


substrate_name: HCN

proof_lines: recombinant enzyme from Saccharomyces cerevisiae

proof_lines: recombinant enzyme from Arabidopsis thaliana

substrate: HCN


enzyme_display_name: streptogrisin B

organism: Streptomyces griseus

substrate_name: Suc-AAPF-p-nitroanilide

enzyme: streptogrisin B

substrate: Suc-AAPF-p-nitroanilide


enzyme_display_name: methionine gamma-lyase

organism: Trichomonas vaginalis

substrate_name: cysteine

enzyme: methionine gamma-lyase

substrate: cysteine


proof_lines:
recombinant, MGL2 C116G
recombinant MGL2, C116G


organism: Homo sapiens


organism: Escherichia coli


enzyme_display_name: clavaminate synthase

organism: Streptomyces antibioticus

enzyme: clavaminate synthase


enzyme_display_name: imidazoleacetate 4-monooxygenase

organism: Pseudomonas sp.

substrate_name: imidazoleacetate

proof_lines: substrate: sodium diethyldithiocarbamate, polarographic method

enzyme: imidazoleacetate 4-monooxygenase

substrate: imidazoleacetate


enzyme_display_name: leucoanthocyanidin reductase

organism: Desmodium uncinatum

assay_conditions_summary: pH 7; 303.15 K; cofactor NADPH

enzyme: leucoanthocyanidin reductase


organism: Onobrychis viciifolia

substrate_name: NADPH

assay_conditions_summary: pH 6.8; 303.15 K; cofactor NADPH

substrate: NADPH


organism: Pseudotsuga menziesii

substrate_name: dihydroquercetin

substrate: dihydroquercetin


organism: Lavandula x intermedia


organism: Pseudomonas monteilii


enzyme_display_name: (R,R)-butanediol dehydrogenase

organism: Corynebacterium glutamicum

proof_lines:
at pH 10.5 and 30°C
at pH 7.0 and 30°C

enzyme: (R,R)-butanediol dehydrogenase


substrate_name: acetoin

assay_conditions_summary: pH 7; cofactor NADPH

proof_lines: cosubstrate NADPH, pH 7.0, temperature not specified in the publication

proof_lines: cosubstrate NADH, pH 7.0, temperature not specified in the publication

substrate: acetoin


organism: Klebsiella aerogenes

substrate_name: NAD+

substrate: NAD+


organism: Klebsiella pneumoniae

proof_lines:
enzyme DhaD, at pH 9.0 and 30°C
enzyme GldA, at pH 9.0 and 30°C


enzyme_display_name: demethylmacrocin O-methyltransferase

organism: Streptomyces fradiae

substrate_name: S-adenosylmethionine

proof_lines:
fixed substrate demethylmacrocin
fixed substrate demethyllactenocin
fixed substrate S-adenosylmethionine

enzyme: demethylmacrocin O-methyltransferase

substrate: S-adenosylmethionine


organism: Neurospora crassa

substrate_name: [cytochrome c]-L-lysine

proof_lines: residue 1-65 or residue 66-104 of horse heart cytochrome c

substrate: [cytochrome c]-L-lysine


organism: Saccharomyces cerevisiae

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


organism: Triticum aestivum

substrate_name: [apocytochrome c]-L-lysine

substrate: [apocytochrome c]-L-lysine


organism: Paenibacillus polymyxa

substrate_name: NADH

proof_lines:
pH 7.0, 30°C
(S)-acetoin, pH 7.0, 30°C

substrate: NADH


organism: Pyrococcus furiosus


enzyme_display_name: protein-glutamate O-methyltransferase

organism: Bacillus subtilis

proof_lines:
MCP methyltransferase I
MCP methyltransferase II

enzyme: protein-glutamate O-methyltransferase


organism: Bos taurus

substrate_name: [histone]-L-glutamate

substrate: [histone]-L-glutamate


organism: Homo sapiens

substrate_name: Unknown

substrate: Unknown


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: protein L-glutamate

substrate: protein L-glutamate


enzyme_display_name: fluorothreonine transaldolase

organism: Streptomyces sp. MA37

substrate_name: phenylacetaldehyde

enzyme: fluorothreonine transaldolase

substrate: phenylacetaldehyde


enzyme_display_name: D-alanine 2-hydroxymethyltransferase

organism: Pseudomonas sp.

substrate_name: L-tetrahydrofolate

proof_lines: pH 7.5, calculated on the basis of one isomer

enzyme: D-alanine 2-hydroxymethyltransferase

substrate: L-tetrahydrofolate


enzyme_display_name: methylmalonyl-CoA carboxytransferase

organism: Propionibacterium freudenreichii subsp. shermanii

substrate_name: pyruvate

assay_conditions_summary: cofactor CoA

enzyme: methylmalonyl-CoA carboxytransferase

substrate: pyruvate


organism: Propionibacterium sp.

substrate_name: oxaloacetate

substrate: oxaloacetate


enzyme_display_name: 2-hydroxy-3-oxoadipate synthase

organism: Mycobacterium tuberculosis

substrate_name: glyoxylate

enzyme: 2-hydroxy-3-oxoadipate synthase

substrate: glyoxylate


organism: Mycolicibacterium phlei


organism: Rattus norvegicus


organism: Sus scrofa


enzyme_display_name: DNA beta-glucosyltransferase

organism: Tequatrovirus T4

substrate_name: DNA containing 2 5-hydroxymethyl cytosine residues

proof_lines: substrate 5-hydroxymethyl cytosine containing DNA, pH 7.9, 25°C

enzyme: DNA beta-glucosyltransferase


organism: Candida albicans


substrate_name: synthetic peptide based on a mucin-like domain in alpha-dystroglycan


organism: Saccharomyces cerevisiae

substrate_name: dolichyl phosphate D-mannose

substrate: dolichyl phosphate D-mannose


enzyme_display_name: CO-methylating acetyl-CoA synthase

organism: Carboxydothermus hydrogenoformans

substrate_name: acetyl-CoA

assay_conditions_summary: pH 7.2; 298.15 K; cofactor CoA

proof_lines: pH 7.2, 25°C, recombinant ACSCh732, acetyl-CoA/CO exchange activity

proof_lines: pH 7.2, 25°C, recombinant ACSChDELTAN, acetyl-CoA/CO exchange activity

proof_lines: exchange activity: C14 from the carboxyl group of acetyl-CoA with C12 from CO

enzyme: CO-methylating acetyl-CoA synthase

substrate: acetyl-CoA


organism: Clostridioides difficile

substrate_name: HS-CoA

proof_lines: at 1 atm CO, pH not specified in the publication, temperature not specified in the publication

substrate: HS-CoA


organism: Methanosarcina thermophila

proof_lines: pH 7.2, 25°C, recombinant ACSD beta, acetyl-CoA/CO exchange activity


organism: Neomoorella thermoacetica

substrate_name: CoA

assay_conditions_summary: pH 7.3; 295.15 K; cofactor CoA

proof_lines: pH 7.3, 22°C, acetyl-CoA synthesis from methyl iodide

proof_lines: pH 7.0, 25°C, under anaerobic conditions, noncompetitive with respect to acetyl-CoA

substrate: CoA


enzyme_display_name: NDP-glucose--starch glucosyltransferase

organism: Gracilaria tenuistipitata

substrate_name: glycogen

assay_conditions_summary: cofactor ADP

proof_lines: UDP-glucose utilizing enzyme in presence of 0.5 M citrate

proof_lines: UDP-glucose utilizing enzyme in absence of citrate

enzyme: NDP-glucose--starch glucosyltransferase

substrate: glycogen


organism: Guillardia theta

substrate_name: ADP-glucose

assay_conditions_summary: pH 6.5; 328.15 K; cofactor ADP

substrate: ADP-glucose


organism: Oryza sativa

proof_lines: enzyme monomer, pH not specified in the publication, temperature not specified in the publication

proof_lines: enzyme oligomer, pH not specified in the publication, temperature not specified in the publication


organism: Pyrococcus furiosus

substrate_name: dTDP-glucose

substrate: dTDP-glucose


enzyme_display_name: flavone apiosyltransferase

organism: Petroselinum crispum

enzyme: flavone apiosyltransferase


enzyme_display_name: ganglioside galactosyltransferase

organism: Gallus gallus

substrate_name: UDP-galactose

enzyme: ganglioside galactosyltransferase

substrate: UDP-galactose


organism: Lithobates pipiens


organism: Neisseria meningitidis

substrate_name: UDP-N-acetyl-D-glucosamine

proof_lines:
UDP-N-acetyl-D-glucosamine as donor
UDP-N-acetyl-D-galactosamine as donor

substrate: UDP-N-acetyl-D-glucosamine


enzyme_display_name: cephaeline 6'-O-methyltransferase

organism: Carapichea ipecacuanha

substrate_name: S-adenosyl-L-methionine

enzyme: cephaeline 6'-O-methyltransferase

substrate: S-adenosyl-L-methionine


enzyme_display_name: monacolin J acid methylbutanoate transferase

organism: Aspergillus terreus

assay_conditions_summary: pH 7.9; 298.15 K; cofactor CoA

proof_lines: pH and temperature not specified in the publication


substrate_name: monacolin J acid

proof_lines: mutant I4N/A9V/K26E/R28S/I35L/N43R/D96R/S109C/A123P/M157V/S164G/S172N/L174F/A178L/N191G/L192I/Q241M/A247S/R250K/S256T/A261H/G275S/Q297G/L335M/L361M/V370I/A383V/N391S/H404K, pH not specified in the publication, temperature not specified in t

enzyme: monacolin J acid methylbutanoate transferase

substrate: monacolin J acid


proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


enzyme_display_name: very-long-chain ceramide synthase

organism: Arabidopsis thaliana

substrate_name: phytosphingosine

enzyme: very-long-chain ceramide synthase

substrate: phytosphingosine


organism: Mus musculus

substrate_name: D-erythro-sphinganine

proof_lines: pH 7.5, 37°C, recombinant enzyme, in presence of inhibitor sphingosine 1-phosphate

substrate: D-erythro-sphinganine


enzyme_display_name: glycogenin glucosyltransferase

organism: Neurospora crassa

substrate_name: UDP-glucose

enzyme: glycogenin glucosyltransferase

substrate: UDP-glucose


organism: Methanocaldococcus jannaschii

substrate_name: 5-phospho-alpha-D-ribose 1-diphosphate

proof_lines: pH 4.8, 70°C, uncompetitive inhibition under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate

proof_lines: pH 4.8, 70°C, uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate

substrate: 5-phospho-alpha-D-ribose 1-diphosphate


organism: Escherichia coli

proof_lines: 37°C, 50 mM HEPES, 50 mM KCl, 10 mM MgCl2 and pH 7.5, 0.1% Triton X-100


organism: Bacteroides thetaiotaomicron

substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


organism: Staphylococcus aureus

substrate_name: L-glutamate

substrate: L-glutamate


enzyme_display_name: L-ascorbate peroxidase

organism: Citrus x limon

enzyme: L-ascorbate peroxidase


organism: Gossypium hirsutum

substrate_name: L-ascorbate

substrate: L-ascorbate


organism: Nicotiana tabacum


organism: Oryza sativa

substrate_name: H2O2; L-Ascorbate

substrate: H2O2; L-Ascorbate


organism: Populus tomentosa


assay_conditions_summary: pH 7

proof_lines: pH 7.0, temperature not specified in the publication


enzyme_display_name: carotenoid isomerooxygenase

organism: Galleria mellonella

substrate_name: alpha-carotene

enzyme: carotenoid isomerooxygenase

substrate: alpha-carotene


organism: Mycobacterium tuberculosis


enzyme_display_name: flavonoid 3',5'-hydroxylase

organism: Billardiera heterophylla

substrate_name: apigenin

enzyme: flavonoid 3',5'-hydroxylase

substrate: apigenin


organism: Adineta vaga

substrate_name: DNA cytosine

proof_lines: pH 7.5, 22°C, enzyme mutant N4CMT79-324, in presence of 0.04 mM SAM

proof_lines: pH 7.5, 22°C, enzyme mutant N4CMT79-324, in presence of 0.2 mM SAM

substrate: DNA cytosine


organism: Aquifex aeolicus

substrate_name: guanine27 in tRNATyr

proof_lines: pH 7.5, 55°C, tRNA Tyr with wild-type sequence adenine26/guanine27 and mutation C43U

proof_lines: pH 7.5, 55°C, wild-type tRNATyr contains the sequence adenine26/guanine27

proof_lines: pH 7.5, 55°C, mutant tRNATyr in which the wilde-type adenine26/guanine27 sequence is substituted with guanine26/adenine27

substrate: guanine27 in tRNATyr


organism: Thalassiosira pseudonana


organism: Carapichea ipecacuanha

substrate_name: N-deacetylisoipecoside

proof_lines: pH 7.5, 30°C, cosubstrate: N-deacetylisoipecoside

substrate: N-deacetylisoipecoside


substrate_name: O-phospho-L-serine

substrate: O-phospho-L-serine


enzyme_display_name: [Src-family] C-terminal protein kinase

organism: Homo sapiens

substrate_name: KKKKEEIYFFF

proof_lines: in the presence of Mn2+, at pH 7.4 and 30°C

proof_lines: GST-tagged recombinant catalytic domain of the enzyme, at pH 7.4 and 30°C

enzyme: [Src-family] C-terminal protein kinase

substrate: KKKKEEIYFFF


enzyme_display_name: [RNA-polymerase]-subunit kinase

organism: Mus musculus

substrate_name: ATP

assay_conditions_summary: pH 6; cofactor ATP

proof_lines: recombinant enzyme, pH 6.0-7.5, temperature not specified in the publication

enzyme: [RNA-polymerase]-subunit kinase

substrate: ATP


assay_conditions_summary: cofactor ATP


substrate_name: CTP

substrate: CTP


substrate_name: N-acetylneuraminic acid

substrate: N-acetylneuraminic acid


organism: Oncorhynchus mykiss

proof_lines: cosubstrate N-glycolylneuraminic acid, pH 9, 25°C

proof_lines: cosubstrate N-acetylneuraminic acid, pH 9, 25°C


enzyme_display_name: chlorophyllase

organism: Brassica oleracea

substrate_name: chlorophyll a

proof_lines: wild type isoform CLH1, in 100 mM sodium phosphate, pH 7.4, at 40°C

enzyme: chlorophyllase

substrate: chlorophyll a


substrate_name: chlorophyll b

proof_lines: wild type isoform CLH2, in 100 mM sodium phosphate, pH 7.4, at 40°C

substrate: chlorophyll b


substrate_name: pheophorbide a methyl ester

substrate: pheophorbide a methyl ester


organism: Chlamydomonas reinhardtii


substrate_name: bacteriochlorophyll a

substrate: bacteriochlorophyll a


organism: Citrus sinensis

substrate_name: H2O; Chlorophyll a

substrate: H2O; Chlorophyll a


organism: Crocosphaera subtropica ATCC 51142


organism: Triticum aestivum


enzyme_display_name: Serratia marcescens nuclease

organism: Serratia marcescens

substrate_name: Unknown

enzyme: Serratia marcescens nuclease

substrate: Unknown


organism: Ginkgo biloba


organism: Oxynema acuminatum


organism: Solanum lycopersicum

substrate_name: pheophytin a

substrate: pheophytin a


proof_lines:
wild-type, pH 8, 25°C
wild-type, presence of Ca2+, pH 8, 25°C
wild-type, presence of EDTA, pH 8, 25°C


enzyme_display_name: carboxymethylcellulase

organism: Alicyclobacillus acidocaldarius

substrate_name: carboxymethyl cellulose

proof_lines: mutant D469V, pH 5.5, 60°C, calculated as amounts of reducing equivalents

enzyme: carboxymethylcellulase

substrate: carboxymethyl cellulose


proof_lines: wild-type, pH 5.5, 60°C, calculated as amounts of reducing equivalents


proof_lines: mutant D471E, pH 5.5, 60°C, calculated as amounts of reducing equivalents


proof_lines: mutant D468E, pH 5.5, 60°C, calculated as amounts of reducing equivalents


proof_lines: mutant D469A, pH 5.5, 60°C, calculated as amounts of reducing equivalents


proof_lines: mutant D468A, pH 5.5, 60°C, calculated as amounts of reducing equivalents


proof_lines: mutant D468V, pH 5.5, 60°C, calculated as amounts of reducing equivalents


proof_lines: mutant D469E, pH 5.5, 60°C, calculated as amounts of reducing equivalents


proof_lines: mutant D471A, pH 5.5, 60°C, calculated as amounts of reducing equivalents


organism: Thermoascus aurantiacus


organism: Ignisphaera aggregans

substrate_name: lupin galactan

substrate: lupin galactan


enzyme_display_name: cathepsin F

enzyme: cathepsin F


enzyme_display_name: phosphoribosyl-AMP cyclohydrolase

organism: Methanococcus vannielii

proof_lines: mutant enzyme H110A, pH and temperature not specified in the publication

enzyme: phosphoribosyl-AMP cyclohydrolase


proof_lines: mutant enzyme D92E, pH and temperature not specified in the publication


proof_lines: mutant enzyme D94E, pH and temperature not specified in the publication


proof_lines: wild-type enzyme, pH and temperature not specified in the publication


enzyme_display_name: mRNA 5'-phosphatase

organism: Saccharomyces cerevisiae

substrate_name: pppA-terminated poly(A)

enzyme: mRNA 5'-phosphatase

substrate: pppA-terminated poly(A)


organism: Semliki forest virus

substrate_name: a 5'-triphospho-[mRNA]

proof_lines: Nsp2-N (N-terminal fragment), pH 7.0, 25°C

substrate: a 5'-triphospho-[mRNA]


enzyme_display_name: prenyl-diphosphate phosphatase

organism: Methanosarcina mazei

substrate_name: prenyl diphosphate

enzyme: prenyl-diphosphate phosphatase

substrate: prenyl diphosphate


enzyme_display_name: phosphohistidine phosphatase

organism: Homo sapiens

assay_conditions_summary: pH 8; 310.15 K; buffer 50 mM

proof_lines: pH 8.0, 37°C, HEPES buffer (50 mM at pH 8.0) with 10 mM NaCl, 0.01% w/v Brij 35 and 0.5 mM DTT as additives

proof_lines: pH and temperature not specified in the publication

enzyme: phosphohistidine phosphatase


enzyme_display_name: adenylate cyclase

organism: Yersinia pestis

assay_conditions_summary: pH 8.5; 310.15 K; cofactor ATP

proof_lines: mutant enzyme C83A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme C83A, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C

enzyme: adenylate cyclase


proof_lines: mutant enzyme E10Q, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme E10Q, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C


proof_lines: mutant enzyme R63A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme R63A, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C


proof_lines: mutant enzyme F5A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme F5A, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C


proof_lines: mutant enzyme K76A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme K76A, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C


assay_conditions_summary: pH 7; 310.15 K; buffer Tris; cofactor ATP

proof_lines: wild type enzyme, at pH 7.0, in Tris buffer at 37°C

proof_lines: wild type enzyme, at pH 7.5, in Tris buffer at 37°C

proof_lines: wild type enzyme, at pH 8.0, in Tris buffer at 37°C


enzyme_display_name: guanylate cyclase

organism: Bos taurus

substrate_name: GTP

proof_lines: purified recombinant sGC in presence of 3-ethyl-3-(ethylaminoethyl)-1-hydroxy-2-oxo-1-triazene

enzyme: guanylate cyclase

substrate: GTP


enzyme_display_name: D-sedoheptulose-7-phosphate isomerase

organism: Burkholderia pseudomallei

substrate_name: D-Sedoheptulose 7-phosphate

enzyme: D-sedoheptulose-7-phosphate isomerase

substrate: D-Sedoheptulose 7-phosphate


substrate_name: D-sedoheptulose 7-phosphate

proof_lines: mutant enzyme D61A, in 20 mM HEPES, pH 8.0, 10 mM MgCl2, 10 mM KCl, at 37°C

substrate: D-sedoheptulose 7-phosphate


proof_lines: mutant enzyme H64Q, in 20 mM HEPES, pH 8.0, 10 mM MgCl2, 10 mM KCl, at 37°C


proof_lines: wild type enzyme, in 20 mM HEPES, pH 8.0, 10 mM MgCl2, 10 mM KCl, at 37°C


organism: Escherichia coli

assay_conditions_summary: pH 8


organism: Helicobacter pylori

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Mycobacterium tuberculosis


proof_lines: pH 8.0, 22°C, recombinant wild-type enzyme


enzyme_display_name: clavaminate synthase

organism: Streptomyces clavuligerus

substrate_name: N-alpha-acetyl-L-arginine

proof_lines:
pH 7.0, 22°C
pH 7.0, 40°C
isozyme CS1, pH 7.0, 22°C

enzyme: clavaminate synthase

substrate: N-alpha-acetyl-L-arginine


enzyme_display_name: imidazoleacetate 4-monooxygenase

organism: Unknown

substrate_name: imidazoleacetate

enzyme: imidazoleacetate 4-monooxygenase

substrate: imidazoleacetate


enzyme_display_name: cortisol sulfotransferase

enzyme: cortisol sulfotransferase


enzyme_display_name: peptidyl-dipeptidase B

enzyme: peptidyl-dipeptidase B


organism: Clonorchis sinensis

proof_lines: isozyme CF-4, in 100 mM sodium phosphate (pH 6.5), at 37°C

proof_lines: isozyme CF-11, in 100 mM sodium phosphate (pH 5.5), at 37°C


enzyme_display_name: atrolysin C

organism: Crotalus atrox

substrate_name: acetyl-Val-Ala-Leu-Gly-Gly

proof_lines: isozyme c, acetyl-Val-Ala-Leu-Gly-Ala, 2-aminobenzoyl-Ala-Gly-Leu-Ala 4-nitrobenzyl amide

enzyme: atrolysin C

substrate: acetyl-Val-Ala-Leu-Gly-Gly


enzyme_display_name: complement factor D

substrate_name: Benzyloxycarbonyl-Lys-thiobenzyl ester

enzyme: complement factor D

substrate: Benzyloxycarbonyl-Lys-thiobenzyl ester


enzyme_display_name: monacolin J acid methylbutanoate transferase

organism: Aspergillus terreus

substrate_name: monacolin J

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: kcat is 0.62 min(-1) for simvastatin synthesis.

substrate: monacolin J


organism: Aspergillus terreus (strain NIH 2624 / FGSC A1156)

enzyme: monacolin J acid methylbutanoate transferase


organism: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

substrate_name: PRPP

proof_lines: kcat is 0.23 sec(-1) with pABA as substrate.

substrate: PRPP


organism: Escherichia coli (strain K12)

substrate_name: phospho-Hep2-Kdo2-lipid A

proof_lines: kcat is 0.41 sec(-1) with phospho-Hep2-Kdo2-lipid A as substrate. kcat is 0.028 sec(-1) with O-deacylated phospho-Hep2-Kdo2-lipid A. kcat is 0.07 sec(-1) with fully deacylated phospho-Hep2-Kdo2-lipid A.

substrate: phospho-Hep2-Kdo2-lipid A


substrate_name: UDP-N-acetylglucosamine

proof_lines: kcat is 0.21 min(-1) with UDP-N-acetylglucosamine. kcat is 0.20 min(-1) with dolichol phosphate.

substrate: UDP-N-acetylglucosamine


organism: Methanococcus vannielii

substrate_name: phosphoribosyl-AMP

substrate: phosphoribosyl-AMP


organism: Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)


organism: Homo sapiens

proof_lines: kcat is 0.78 sec(-1) for (2E)-geranyl diphosphate as substrate (at pH 8.0 and 22 degrees Celsius) (PubMed:38944687). kcat is 0.55 sec(-1) with (R)-5-diphosphomevalonate as substrate (at pH 8.0 and 22 degrees Celsius) (PubMed:38944687). kcat


substrate_name: dGTP (at pH 7.5)

proof_lines: Has a 10-fold higher catalytic efficiency toward dGTP compared to 8-oxo-dGTP. kcat is 0.58 sec(-1) with dGTP as substrate (at pH 8.0 and 22 degrees Celsius) (PubMed:38944687). kcat is 0.72 sec(-1) with (2E)-geranyl diphosphate as substrate

substrate: dGTP (at pH 7.5)


organism: Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)

substrate_name: dimethylallyl diphosphate (DMAPP) (at pH 8.5 and 30 degrees Celsius)

proof_lines: kcat is 0.139 sec(-1) with DMAPP as substrate. kcat is 0.681 sec(-1) with as IPP substrate. kcat is 0.395 sec(-1) with GPP as substrate. kcat is 0.298 sec(-1) with FPP as substrate.


enzyme_display_name: imidazoleglycerol-phosphate dehydratase

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: imidazoleglycerol-phosphate (at pH 7.7 and 37 degrees Celsius)

proof_lines: kcat is 1000 sec(-1) with imidazoleglycerol-phosphate as substrate (at pH 7.7 and 37 degrees Celsius).

enzyme: imidazoleglycerol-phosphate dehydratase


enzyme_display_name: L-ascorbate peroxidase

organism: Galdieria partita

substrate_name: L-ascorbate

enzyme: L-ascorbate peroxidase

substrate: L-ascorbate


organism: Methanothermobacter thermautotrophicus


proof_lines:
wild type enzyme, at pH 6.8 and 70°C
wild type enzyme, at pH 6.8 and 50°C


enzyme_display_name: mycoredoxin

organism: Corynebacterium glutamicum

substrate_name: 2-hydroxyethyl disulfide-mycothiol

proof_lines: Ncgl2478mutant enzyme C21S, at pH 7.6 and 30°C

enzyme: mycoredoxin

substrate: 2-hydroxyethyl disulfide-mycothiol


proof_lines: wild type enzyme Ncgl2478, at pH 7.6 and 30°C

proof_lines: wild type enzyme NCgl0018, at pH 7.5 and 37°C

proof_lines: wild type enzyme Mrx1, at pH 7.6 and 30°C


proof_lines: Ncgl2478mutant enzyme C24S, at pH 7.6 and 30°C


organism: Lathyrus oleraceus

substrate_name: guaiacol

proof_lines:
will-type enzyme
enzyme form C
enzyme form B

substrate: guaiacol


organism: Leishmania major

substrate_name: ascorbate

substrate: ascorbate


substrate_name: cytochrome c

substrate: cytochrome c


proof_lines: wild-type, Cys32Ser mutant and DTNB-modified enzyme


proof_lines: NCgl0018 mutant enzyme C107S, at pH 7.5 and 37°C


proof_lines: NCgl0018 mutant enzyme C104S, at pH 7.5 and 37°C


enzyme_display_name: 3-methylquercetin 7-O-methyltransferase

organism: Populus sp.

substrate_name: kaempferol

assay_conditions_summary: pH 7.5; 310.15 K; buffer 10 mM Tris/HCl

proof_lines: wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C

enzyme: 3-methylquercetin 7-O-methyltransferase

substrate: kaempferol


proof_lines: mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C


substrate_name: D-mannose 6-phosphate

substrate: D-mannose 6-phosphate


enzyme_display_name: [Src-family] C-terminal protein kinase

substrate_name: [Src]-(L-tyrosine)

proof_lines: catalytic domain of the enzyme with all grafted Src elements (DELTAI255/DELTAL262/M129R/C-loop/alpha-helix C region/beta4.5 turn), at pH 8.0 and 30°C

enzyme: [Src-family] C-terminal protein kinase

substrate: [Src]-(L-tyrosine)


proof_lines: catalytic domain of the enzyme, at pH 8.0 and 30°C


organism: Brassica rapa


organism: Chlorella vulgaris


organism: Cucumis metuliferus


enzyme_display_name: cathepsin F

proof_lines: pH 6.5, room temperature, wild-type enzyme

enzyme: cathepsin F


proof_lines: pH 6.5, room temperature, mutant enzyme N97Q/N108Q/N170Q


organism: Euglena gracilis

assay_conditions_summary: pH 7; 295.15 K; buffer 50 mM potassium phosphate

proof_lines: recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C


organism: Glycine max

substrate_name: pyrogallol

proof_lines:
recombinant enzyme
recombinant enzyme 1
recombinant enzyme 2

substrate: pyrogallol


organism: Helicoverpa zea


organism: Musa x paradisiaca


organism: Nicotiana tabacum

proof_lines:
pH 6.4, untreated cells
pH 6.4, heat-shock cells


organism: Oryza sativa

proof_lines:
pH 7.0, APX 2
pH 6.5, APX 1


organism: Pallavicinia lyellii

proof_lines: in 50 mM potassium phosphate (pH 7.0), at 22°C


organism: Solanum tuberosum


organism: Zea mays


organism: Panicum virgatum

proof_lines: pH 7.0, temperature not specified in the publication. Hill coefficient n is 1.0

proof_lines: pH 7.0, temperature not specified in the publication. Non-Michaelis-Menten kinetics, Hill ccoefficient n is 1.6


organism: Raphanus sativus


organism: Ricinus communis


organism: Saccharomyces cerevisiae


enzyme_display_name: carotenoid isomerooxygenase

organism: Plesiocystis pacifica SIR-1

enzyme: carotenoid isomerooxygenase


enzyme_display_name: 2,4-dichlorophenol 6-monooxygenase

organism: Soil bacterium

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

enzyme: 2,4-dichlorophenol 6-monooxygenase


organism: Acinetobacter sp.

assay_conditions_summary: cofactor NADPH


organism: Burkholderia cepacia

substrate_name: NADPH

substrate: NADPH


organism: Cupriavidus necator

substrate_name: NADH

proof_lines:
+ 2,4-dichlorophenol
cosubstrate NADPH
cosubstrate NADH

substrate: NADH


organism: Pseudomonadota


organism: Bacillus amyloliquefaciens


organism: Chroomonas mesostigmatica


organism: Gynuella sunshinyii

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


organism: Nisaea denitrificans


organism: Ostreococcus virus


enzyme_display_name: N-terminal methionine N(alpha)-acetyltransferase NatB

organism: Homo sapiens

substrate_name: MLALISRRWGRPVGRRRRRPVRVYP

substrate: MLALISRRWGRPVGRRRRRPVRVYP


organism: Chrysosplenium americanum


organism: Prymnesium parvum

proof_lines: 22°C, pH not specified in the publication

proof_lines: 22°, pH not specified in the publication


organism: Ulva intestinalis


enzyme_display_name: very-long-chain ceramide synthase

substrate_name: behenoyl-CoA

assay_conditions_summary: pH 7.4; 310.15 K; cofactor CoA

enzyme: very-long-chain ceramide synthase

substrate: behenoyl-CoA


organism: Methanosarcina thermophila

proof_lines:
pH 4.8, 50°C
pH 4.8, 50°C, Ki(slope)


enzyme_display_name: glycogenin glucosyltransferase

organism: Oryctolagus cuniculus

substrate_name: UDP-glucose

proof_lines: non-glucosylated protein, mass sprectrometry assay

proof_lines: glucosylated protein, 14C-glucose incorporation assay

proof_lines: glucosylated protein, 4-10 interval, mass sprectrometry assay

enzyme: glycogenin glucosyltransferase

substrate: UDP-glucose


organism: Rattus norvegicus

substrate_name: n-dodecyl-beta-D-maltoside

substrate: n-dodecyl-beta-D-maltoside


organism: Gallus gallus

substrate_name: lactosylceramide

proof_lines:
at 0.2 mM
at 0.1 mM

substrate: lactosylceramide


substrate_name: CMP-N-acetylneuraminate

substrate: CMP-N-acetylneuraminate


organism: Mesocricetus auratus


organism: Oncorhynchus mykiss


enzyme_display_name: zeatin 9-aminocarboxyethyltransferase

organism: Lupinus luteus

substrate_name: O-glycosylzeatin

enzyme: zeatin 9-aminocarboxyethyltransferase

substrate: O-glycosylzeatin


enzyme_display_name: homospermidine synthase

organism: Acinetobacter tartarogenes

substrate_name: putrescine

enzyme: homospermidine synthase

substrate: putrescine


organism: Blastochloris viridis

proof_lines:
Vmax: 0.00132 mmol/min/mg
in presence of 1 mM spermidine
in presence of 1 mM putrescine


organism: Lathyrus sativus


enzyme_display_name: protein geranylgeranyltransferase type II

substrate_name: Unknown

proof_lines: 9 microM (3,7,11-trimethyl-12-(7-nitrobenzo[1,2,5]oxadiazo-4-ylamino)-dodeca-2,6,19-trien-1) diphosphate, 3 microM Rab7, 3 microM Rab escort protein, 400 nM enzyme

proof_lines: mixed-type inhibition with geranylgeranyl diphosphate 1 as variable substrate, 50 mM sodium HEPES, pH 7.2, 5 mM MgCl2, 1 mM Nonidet P-40, 1 mM dithioerythritol, 4 microM Rab1a, 50 nM enzyme, 20 min, 37°C

substrate: Unknown


substrate_name: Rab protein

enzyme: protein geranylgeranyltransferase type II

substrate: Rab protein


organism: Saccharomyces cerevisiae


enzyme_display_name: asparagine--oxo-acid transaminase

organism: Mus musculus

substrate_name: L-methionine

proof_lines:
cosubstrate phenylpyruvate
cosubstrate 2-oxosuccinamate

enzyme: asparagine--oxo-acid transaminase

substrate: L-methionine


enzyme_display_name: [RNA-polymerase]-subunit kinase

substrate_name: ATP

assay_conditions_summary: pH 7.5; 300.15 K; cofactor ATP

proof_lines:
27°C, pH 7.5
30°C, pH 7.9, kinase CTDK1
30°C, pH 7.9, kinase CTDK2

enzyme: [RNA-polymerase]-subunit kinase

substrate: ATP


substrate_name: GTP

assay_conditions_summary: pH 7.9; 303.15 K; cofactor ATP

substrate: GTP


assay_conditions_summary: pH 7.8; cofactor ATP


enzyme_display_name: thyroid-hormone transaminase

proof_lines: cosubstrate triiodothyronine, pH 7.8, 37°C

enzyme: thyroid-hormone transaminase


organism: Streptomyces griseus

substrate_name: beta-Alanine

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: beta-Alanine


organism: Ureibacillus massiliensis

proof_lines: cosubstrate beta-alanine, pH 8, temperature not specified in the publication

proof_lines: cosubstrate 2-oxoglutarate, pH 8, temperature not specified in the publication


enzyme_display_name: nucleotide diphosphokinase

organism: Streptomyces aspergilloides

substrate_name: ADP

assay_conditions_summary: pH 10; 310.15 K; cofactor ADP

enzyme: nucleotide diphosphokinase

substrate: ADP


organism: Streptomyces morookaense

substrate_name: GDP

proof_lines:
pH 10.0, 37°C, reaction with pppApp
pH 10.0, 37°C, reaction with dATP
pH 10.0, 37°C, reaction with ATP

substrate: GDP


organism: Escherichia coli

assay_conditions_summary: 295.15 K; cofactor ATP

proof_lines: pH not specified in the publication, 22°C


organism: Sus scrofa

substrate_name: UDP-GlcNAc

substrate: UDP-GlcNAc


organism: Acanthamoeba castellanii


organism: Bos taurus


organism: Glycine max


substrate_name: dolichyl phosphate

substrate: dolichyl phosphate


enzyme_display_name: GDP polyribonucleotidyltransferase

organism: vesicular stomatitis virus

enzyme: GDP polyribonucleotidyltransferase


enzyme_display_name: scymnol sulfotransferase

organism: Heterodontus portusjacksoni

proof_lines:
room temperature, pH 6.5
pH 6.5
25°C

enzyme: scymnol sulfotransferase


organism: Trygonoptera sp.


organism: Trygonorrhina fasciata


enzyme_display_name: galactosylceramide sulfotransferase

organism: Homo sapiens

substrate_name: galactosylceramide

proof_lines:
pH 6.5, 37°C
at pH 7.1 and 37°C
pH 7.0, 4°C

enzyme: galactosylceramide sulfotransferase

substrate: galactosylceramide


organism: Rattus norvegicus

proof_lines:
pH 6.2, 37°C
pH 6.2, 37°C, para isomer
pH 6.2, 37°C, meta isomer


enzyme_display_name: glycochenodeoxycholate sulfotransferase

substrate_name: lithocholate

enzyme: glycochenodeoxycholate sulfotransferase

substrate: lithocholate


organism: Mesocricetus auratus

substrate_name: glycochenodeoxycholate

proof_lines: female hamster, pH 7.0, 37°C, saturating substrate concentration

proof_lines: female hamster, pH 7.0, 37°C, physiological substrate concentration

substrate: glycochenodeoxycholate


substrate_name: 3beta-hydroxy-5beta-cholanoic acid

substrate: 3beta-hydroxy-5beta-cholanoic acid


enzyme_display_name: cortisol sulfotransferase

organism: Cavia porcellus

enzyme: cortisol sulfotransferase


organism: Gallus gallus

substrate_name: cortisol

substrate: cortisol


proof_lines:
pH 6.8, sulfotransferase I
pH 6.8, sulfotransferase III
pH 6.8, sulfotransferase I and III


enzyme_display_name: chlorophyllase

organism: Auxenochlorella protothecoides

substrate_name: chlorophyll a

enzyme: chlorophyllase

substrate: chlorophyll a


organism: Capsicum annuum

substrate_name: chlorophyll b

substrate: chlorophyll b


organism: Chenopodium album

proof_lines:
type 1 chlorophyllase
type 2 chlorophyllase


organism: Citrus unshiu


organism: Citrus x limon

substrate_name: Chlorophyll

substrate: Chlorophyll


organism: Phaeodactylum tricornutum

substrate_name: pheophytin

assay_conditions_summary: pH 8; buffer pH 8

proof_lines: immobilized enzyme in 20 mM sodium phosphate buffer pH 8

substrate: pheophytin


organism: Prunus cerasus

proof_lines:
isoform Chlase F2, at pH 7.0 and 30°C
isoform Chlase F1, at pH 7.0 and 30°C


organism: Prunus domestica

proof_lines:
isoform Chlase F1, at pH 7.0 and 30°C
isoform Chlase F2, at pH 7.0 and 30°C


organism: Prunus padus


organism: Secale cereale

substrate_name: bacteriochlorophyll a

substrate: bacteriochlorophyll a


enzyme_display_name: choline sulfotransferase

organism: Aspergillus nidulans

substrate_name: N,N-dimethylethylaminoethanol

enzyme: choline sulfotransferase

substrate: N,N-dimethylethylaminoethanol


organism: Limonium perezii

substrate_name: choline

substrate: choline


organism: Penicillium chrysogenum

proof_lines: pH 7.8, 37°C, N,N-dimethylaminoethanol as substrate

proof_lines: pH 7.8, 37°C depending on the choline concentration


enzyme_display_name: N-acetylgalactosaminoglycan deacetylase

organism: Aspergillus parasiticus

substrate_name: N-acetylated polygalactosamine

enzyme: N-acetylgalactosaminoglycan deacetylase

substrate: N-acetylated polygalactosamine


organism: Escherichia coli

substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication

substrate: Unknown


organism: Neurospora crassa

substrate_name: N-acetylgalactosamine

proof_lines:
35°C, pH 9
35°C, pH 5

substrate: N-acetylgalactosamine


organism: Staphylococcus epidermidis

substrate_name: 3-carboxyumbelliferyl acetate

substrate: 3-carboxyumbelliferyl acetate


enzyme_display_name: Serratia marcescens nuclease

organism: Serratia marcescens

substrate_name: B-Z-hybrid form DNA

proof_lines: pH 8.0, 30°C, in presence of Co(NH3)6 3+

enzyme: Serratia marcescens nuclease

substrate: B-Z-hybrid form DNA


enzyme_display_name: protein-glutamate methylesterase

organism: Bacillus subtilis

substrate_name: methylated methyl-accepting chemotaxis protein

enzyme: protein-glutamate methylesterase


organism: Rattus norvegicus

substrate_name: several methylated protein substrates

substrate: several methylated protein substrates


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: methyl-accepting chemotaxis protein

proof_lines: pH 7.0, 25°C, in absence of phosphoramidate

proof_lines: pH 7.0, 25°C, in presence of phosphoramidate

substrate: methyl-accepting chemotaxis protein


enzyme_display_name: [myosin-light-chain] phosphatase

organism: Badhamia polycephala

substrate_name: [myosin light-chain] phosphate

proof_lines: pH 7.6, temperature not specified in the publication

enzyme: [myosin-light-chain] phosphatase

substrate: [myosin light-chain] phosphate


organism: Gallus gallus

substrate_name: Phosphorylated myosin

proof_lines: at a ionic strength of 0.15 M, in the presence of 0.1 mM MgATP

substrate: Phosphorylated myosin


organism: Mammalia

substrate_name: myosin light-chain phosphate

proof_lines: smooth muscle, type 1 protein phosphatase delta and MYPT1

proof_lines: smooth muscle, type 1 protein phosphatase delta and MYPT2

proof_lines: cardiac muscle, type 1 protein phosphatase delta and MYPT2

substrate: myosin light-chain phosphate


organism: Meleagris gallopavo

substrate_name: myosin light-chain

proof_lines:
pH 7, 30°C, catalytic subunit of SMP-I
pH 7, 30°C, enzyme SMP-IV
pH 7, 30°C, SMP-I holoenzyme

substrate: myosin light-chain


organism: Acremonium sp.

substrate_name: hesperidin

proof_lines: immobilized enzyme, pH 5.0, 60°C, the change in the affinity of the enzyme to its substrate compared to the free enzyme is probably caused by structural changes introduced by the immobilization process and by the lower accessibility to the

substrate: hesperidin


organism: Acremonium sp. DSM 24697

proof_lines: isoform alphaRbetaG I, at pH 5.0 and 55°C

proof_lines: isoform alphaRbetaG II, at pH 5.0 and 40°C


enzyme_display_name: ginsenosidase type I

organism: Aspergillus niger

substrate_name: ginsenoside Rb3

proof_lines: pH 5.0, 45°C, formation of compound Mx-1

proof_lines: pH 5.0, 45°C, formation of ginsenoside Rd

enzyme: ginsenosidase type I

substrate: ginsenoside Rb3


organism: Paenibacillus mucilaginosus

substrate_name: ginsenoside Rg3

substrate: ginsenoside Rg3


enzyme_display_name: glucan 1,4-alpha-maltotetraohydrolase

organism: Roseateles saccharophilus

substrate_name: soluble starch

proof_lines: isoform maltotetraohydolase I, pH 7.5, 55°C

proof_lines: isoform maltotetraohydolase II, pH 7.5, 55°C

enzyme: glucan 1,4-alpha-maltotetraohydrolase

substrate: soluble starch


organism: Stutzerimonas stutzeri

substrate_name: oyster glycogen

proof_lines:
F1form
F1 and F2 form
F2 form

substrate: oyster glycogen


organism: Bacillus licheniformis

substrate_name: potato galactan

substrate: potato galactan


organism: Bacillus subtilis subsp. subtilis

proof_lines: pH and temperature not specified in the publication


enzyme_display_name: peptidyl-dipeptidase B

organism: Bos taurus

enzyme: peptidyl-dipeptidase B


enzyme_display_name: N-formylmethionyl-peptidase

substrate_name: N-Formyl-Met-Leu

enzyme: N-formylmethionyl-peptidase

substrate: N-Formyl-Met-Leu


enzyme_display_name: carboxypeptidase E

substrate_name: dansyl-Phe-Ala-Arg

proof_lines: pituitary brain adrenal

enzyme: carboxypeptidase E

substrate: dansyl-Phe-Ala-Arg


organism: Manduca sexta

substrate_name: dansyl-Phe-Leu-Arg

substrate: dansyl-Phe-Leu-Arg


organism: Sus scrofa

substrate_name: iodo-acetyl-Tyr-Ala-Arg

substrate: iodo-acetyl-Tyr-Ala-Arg


enzyme_display_name: deoxyribodipyrimidine endonucleosidase

organism: Micrococcus luteus

substrate_name: DNA

proof_lines:
cytosine labeled DNA
thymine labeled DNA

enzyme: deoxyribodipyrimidine endonucleosidase

substrate: DNA


organism: Paramecium bursaria Chlorella virus 1

proof_lines:
cis-syn 49-bp duplex
trans-syn 49-bp duplex
cis-syn 12-bp duplex


organism: Tequatrovirus T4

proof_lines:
thymine dimersl
arylamidated DNA


enzyme_display_name: complement factor I

organism: Homo sapiens

enzyme: complement factor I


enzyme_display_name: streptogrisin A

organism: Streptomyces griseus

enzyme: streptogrisin A


enzyme_display_name: streptogrisin B

proof_lines:
pH 8.0, 25°C, native SGPB
pH 8.0, 22°C
pH 4.0, 22°C

enzyme: streptogrisin B


enzyme_display_name: glutamyl endopeptidase II

enzyme: glutamyl endopeptidase II


enzyme_display_name: limulus clotting factor C

organism: Tachypleus tridentatus

enzyme: limulus clotting factor C


enzyme_display_name: adenain

organism: Human adenovirus sp.

proof_lines: pH 8.0, 25°C, activation by viral DNA and the 11-amino acid residue peptide from the C-terminus of adenovirus precursor protein and the viral DNA

proof_lines: pH 8.0, 25°C, in absence of any cofactor

enzyme: adenain


organism: unidentified adenovirus


enzyme_display_name: candidapepsin

organism: Candida albicans

substrate_name: albumin

enzyme: candidapepsin

substrate: albumin


organism: Candida parapsilosis


enzyme_display_name: memapsin 1

substrate_name: gamma-secretase cleavage site of notch

enzyme: memapsin 1

substrate: gamma-secretase cleavage site of notch


enzyme_display_name: peptidyl-glutaminase

organism: Niallia circulans

substrate_name: N-acetyl-L-Gln

enzyme: peptidyl-glutaminase

substrate: N-acetyl-L-Gln


enzyme_display_name: pitrilysin

organism: Acinetobacter calcoaceticus

substrate_name: Insulin

enzyme: pitrilysin

substrate: Insulin


organism: Escherichia coli

substrate_name: Substance P

substrate: Substance P


organism: Halalkalibacterium halodurans

substrate_name: dynorphin A fragment 1-13

substrate: dynorphin A fragment 1-13


enzyme_display_name: procollagen N-endopeptidase

organism: Bos taurus

substrate_name: procollagen I

enzyme: procollagen N-endopeptidase

substrate: procollagen I


organism: Gallus gallus

proof_lines:
procollagen I from human
procollagen I from chicken


substrate_name: Unknown

proof_lines:
in the presence of heparin
in the absence of heparin

substrate: Unknown


enzyme_display_name: creatinase

organism: Acinetobacter sp.

substrate_name: Creatine

enzyme: creatinase

substrate: Creatine


organism: Alcaligenes faecalis


organism: Alcaligenes sp.


organism: Paracoccus sp. WB1


organism: Pseudomonas putida


enzyme_display_name: adenosine-phosphate deaminase

organism: Meretrix lusoria

substrate_name: adenosine

enzyme: adenosine-phosphate deaminase

substrate: adenosine


organism: Porphyra crispata

assay_conditions_summary: cofactor NAD+


organism: Todarodes pacificus


organism: Turbo cornutus

substrate_name: 2',3'-Cyclic AMP

substrate: 2',3'-Cyclic AMP


enzyme_display_name: ATP deaminase

organism: Aspergillus glaucus

substrate_name: ATP

assay_conditions_summary: cofactor ATP

enzyme: ATP deaminase

substrate: ATP


organism: Desulfovibrio desulfuricans

substrate_name: ADP

substrate: ADP


organism: Microsporum audouinii


enzyme_display_name: aliphatic (R)-hydroxynitrile lyase

organism: Linum usitatissimum

enzyme: aliphatic (R)-hydroxynitrile lyase


enzyme_display_name: phosphoribosyl-AMP cyclohydrolase

organism: Methanococcus vannielii

enzyme: phosphoribosyl-AMP cyclohydrolase


organism: Methanothermobacter thermautotrophicus

proof_lines: pH 8.5, 65°C, recombinant enzyme with bound Cd2+

proof_lines: pH 8.0, 65°C, recombinant enzyme with bound Zn2+

proof_lines: pH 8.0, 65°C, recombinant enzyme with bound Cd2+


enzyme_display_name: imidazoleglycerol-phosphate dehydratase

organism: Saccharomyces cerevisiae

enzyme: imidazoleglycerol-phosphate dehydratase


organism: Triticum aestivum


organism: Arabidopsis thaliana


organism: Mycobacterium tuberculosis


organism: Neurospora crassa


enzyme_display_name: methionine gamma-lyase

substrate_name: L-methionine

enzyme: methionine gamma-lyase

substrate: L-methionine


organism: Aspergillus sp.


organism: Brevibacterium linens


organism: Citrobacter freundii

substrate_name: DL-homoserine

substrate: DL-homoserine


substrate_name: methiin

proof_lines: liposomes and polymeric vesicles-bound mutant enzyme C115H, at pH 8.0 and 30°C

proof_lines: PEGylated mutant enzyme C115H, at pH 8.0 and 30°C

proof_lines: free mutant enzyme C115H, at pH 8.0 and 30°C

substrate: methiin


organism: Citrobacter intermedius


organism: Cladosporium cladosporioides


organism: Clostridium tetani

substrate_name: L-methionine sulfoxide

substrate: L-methionine sulfoxide


organism: Pseudomonas sp.


organism: Treponema denticola


organism: Trichomonas vaginalis

substrate_name: DL-homocysteine

proof_lines:
recombinant MGL1
recombinant MGL2

substrate: DL-homocysteine


enzyme_display_name: L-3-cyanoalanine synthase

organism: Vicia sativa subsp. nigra

substrate_name: cyanide

enzyme: L-3-cyanoalanine synthase

substrate: cyanide


organism: Zea mays

substrate_name: L-cysteine

substrate: L-cysteine


organism: Zonocerus variegatus

substrate_name: NaCN

substrate: NaCN


organism: Lathyrus latifolius


organism: Lupinus sp.

substrate_name: KCN

substrate: KCN


organism: Solanum tuberosum

substrate_name: O-acetyl-L-serine

substrate: O-acetyl-L-serine


organism: Spinacia oleracea

proof_lines:
26°C, pH 9.8
vs. chloroalanine
vs. L-cysteine


organism: Entamoeba histolytica


organism: Lactococcus lactis


organism: Porphyromonas gingivalis

substrate_name: S-Benzyl-L-cysteine

substrate: S-Benzyl-L-cysteine


organism: Pseudomonas putida

substrate_name: L-ethionine

substrate: L-ethionine


enzyme_display_name: adenylate cyclase

organism: Nocardia erythropolis

enzyme: adenylate cyclase


organism: Oryctolagus cuniculus

assay_conditions_summary: 303.15 K; cofactor ATP

proof_lines: cortical adenylyl cyclase, in the presence of 7 mM Mg2+, at 30°C, pH not specified in the publication

proof_lines: medulla adenylyl cyclase, in the presence of 7 mM Mg2+, at 30°C, pH not specified in the publication

proof_lines: cortical adenylyl cyclase, in the presence of 7 mM Mn2+, at 30°C, pH not specified in the publication


organism: Paramecium tetraurelia


organism: Phycomyces blakesleeanus


organism: Mammalia

assay_conditions_summary: pH 7.4; 303.15 K; cofactor ATP

proof_lines: recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mg2+, 0.1 mM forskolin, stimulatory G protein alpha subunits guanosine 5-(gamma-thio)triphosphate

proof_lines: recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mg2+, stimulatory G protein alpha subunits guanosine 5-(gamma-thio)triphosphate


organism: Mus musculus

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

proof_lines: S49 cyc-, pH 7.4, 37°C, Mn2+, 100 microM forskolin

proof_lines: Sf9, Mn2+, pH 7.4, 37°C, 100 microM forskolin, 10 mM NaF, 10 microM AlCl3

proof_lines: Sf9, Mn2+, 100 microM forskolin, 10 mM NaF, 10 microM AlCl3


organism: Mytilus galloprovincialis

assay_conditions_summary: pH 7.4; 295.15 K; cofactor ATP


organism: Neurospora crassa


organism: Bordetella pertussis

assay_conditions_summary: pH 7.4; 298.15 K; cofactor ATP

proof_lines: pH and temperature not specified in the publication, in absence of calmodulin

proof_lines: pH and temperature not specified in the publication, in presence of calmodulin


organism: Bos taurus


organism: Canis lupus familiaris

proof_lines: recombinant cytosolic domain of type V adenylyl cyclase


organism: Cavia porcellus


organism: Escherichia coli


organism: Euglena gracilis

assay_conditions_summary: 298.15 K; cofactor ATP

proof_lines: 25°C, plasma membrane isolated from lactic acid grown cells

proof_lines: 25°C, plasma membrane isolated from glutamate/malate grown cells


organism: Gallus gallus


organism: Glutamicibacter nicotianae


organism: Synechocystis sp.

proof_lines:
in the presence of Cl-
in the presence of HCO3-
native Cya1, pH 7.5, 27°C, 50 mM NaHCO3


organism: Synechocystis sp. PCC 6803


organism: Pseudomonas aeruginosa

assay_conditions_summary: pH 8.5; 313.15 K; cofactor ATP


organism: Ruditapes philippinarum


organism: Saccharomyces cerevisiae


organism: Sus scrofa


enzyme_display_name: guanylate cyclase

organism: Arbacia punctulata

substrate_name: GTP

enzyme: guanylate cyclase

substrate: GTP


organism: Blastocladiella emersonii

proof_lines: pH 7.5, 22°C, wild-type enzyme, wild-type enzyme


proof_lines: octylglucoside solubilized enzyme, Mn2+-supported enzyme


organism: Dictyostelium discoideum

proof_lines:
soluble guanylyl cyclase sGC
22°C, pH 8.0
guanylyl cyclase GCA


organism: Dictyostelium sp.


organism: Drosophila melanogaster

proof_lines:
under anaerobic conditions
with 0.273 mM oxygen


organism: Rattus norvegicus

proof_lines: pH 8.0, 30°C, wild-type enzyme, in presence of NO-donor S-nitroso-N-acetylpenicillamine


assay_conditions_summary: pH 8; 303.15 K; cofactor ATP

proof_lines: 30°C, pH 8.0, in the presence of N-(beta-D-glucopyranosyl-N2-acetyl-S-nitroso-D,L-penicillaminamide) and YC-1


organism: Tetrahymena pyriformis


enzyme_display_name: amino-acid racemase

organism: Pseudomonas putida

substrate_name: D-ornithine

proof_lines: pH and temperature not specified in the publication, in 20% methanol

proof_lines: pH and temperature not specified in the publication, in 10% methanol

proof_lines: pH and temperature not specified in the publication, in 40% methanol

enzyme: amino-acid racemase

substrate: D-ornithine


organism: Aeromonas caviae

substrate_name: D-Ser

proof_lines: L-Lys L-Arg D-Lys

substrate: D-Ser


organism: Aplysia californica

substrate_name: L-aspartate

assay_conditions_summary: pH 8; 303.15 K; buffer 50 mM Tris-HCl

proof_lines: in 50 mM Tris-HCl buffer, pH 8.0, at 30°C

proof_lines: in 50 mM Tris-HCl buffer, pH 8.5, for Ser reactions, at 30°C

substrate: L-aspartate


organism: Geobacillus kaustophilus

substrate_name: N-succinyl-D-alanine

substrate: N-succinyl-D-alanine


organism: Mus musculus

substrate_name: L-serine

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP

proof_lines: pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction

substrate: L-serine


enzyme_display_name: ribulose-phosphate 3-epimerase

organism: Bos taurus

substrate_name: D-ribulose 5-phosphate

enzyme: ribulose-phosphate 3-epimerase

substrate: D-ribulose 5-phosphate


organism: Spinacia oleracea


enzyme_display_name: aldose 1-epimerase

organism: Aspergillus niger

substrate_name: alpha-D-glucose

enzyme: aldose 1-epimerase

substrate: alpha-D-glucose


proof_lines:
alpha-D-glucose, newborn calf
adult
embryo


organism: Capsicum frutescens


organism: Escherichia coli

substrate_name: D-xylose

substrate: D-xylose


organism: Gallus gallus


organism: Homo sapiens

proof_lines:
kidney
alpha-D-glucose, liver


organism: Lactococcus lactis

substrate_name: alpha-D-quinovose

substrate: alpha-D-quinovose


proof_lines: beta-L-arabinose, enzyme form I from kidney, enzyme form III from liver

proof_lines: alpha-D-galactose, enzyme form I from kidney

proof_lines: beta-L-arabinose, enzyme form II from liver


organism: Rhizopus stolonifer


organism: Sus scrofa

proof_lines: alpha-D-glucose, kidney, embryo and adult


enzyme_display_name: steroid Delta-isomerase

enzyme: steroid Delta-isomerase


organism: Comamonas testosteroni

proof_lines: 25°C, pH 6.5, solvent D2O, mutant enzyme D38A

proof_lines: 25°C, pH 7.5 or 8.5, solvent H2O, mutant enzyme D38A

proof_lines: 25°C, pH 8.5, solvent D2O, mutant enzyme D38A


proof_lines: 22°C, pH 7.4, truncated cytosolic enzyme DEALTA283-310


enzyme_display_name: L-ribulose-5-phosphate 4-epimerase

substrate_name: L-ribulose 5-phosphate

enzyme: L-ribulose-5-phosphate 4-epimerase

substrate: L-ribulose 5-phosphate


organism: Klebsiella aerogenes


enzyme_display_name: methyl-branched lipid omega-hydroxylase

organism: Mycobacterium tuberculosis

substrate_name: CYP124; 15-Methyl-palmitic acid

enzyme: methyl-branched lipid omega-hydroxylase

substrate: CYP124; 15-Methyl-palmitic acid


organism: Nocardia erythropolis


organism: Pseudomonas sp.


enzyme_display_name: chlorophyllase

organism: Triticum aestivum

substrate_name: H2O; Chlorophyll

proof_lines: pH 8.0, 25°C, recombinant enzyme purified by affinity chromatography

proof_lines: pH 8.0, 25°C, recombinant enzyme purified by microtiter plate method

proof_lines: final concentration of 10% v/v acetonitrile at 37°C

enzyme: chlorophyllase

substrate: H2O; Chlorophyll


organism: Clostridium sporogenes

substrate_name: H2O; L-Methionine

substrate: H2O; L-Methionine


substrate_name: ATP

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

proof_lines: isoform AC2, in 75 mM Tris/HCl, pH 7.4, at 37°C

proof_lines: isoform AC1, in 75 mM Tris/HCl, pH 7.4, at 37°C

substrate: ATP


organism: Rattus norvegicus

proof_lines: after activation of soluble AC with the stimulatory G protein alpha protein and GTP-gamma-S in the presence of Mn2+

proof_lines: after activation of soluble AC with the stimulatory G protein alpha protein and GTP-gamma-S in the presence of Mg2+


organism: Spodoptera frugiperda


enzyme_display_name: thioglucosidase

organism: Raphanus sativus

substrate_name: H2O; Sinigrin

proof_lines:
in absence of ascorbate
in presence of 0.5 mM ascorbate

enzyme: thioglucosidase

substrate: H2O; Sinigrin


enzyme_display_name: L-ascorbate peroxidase

organism: Lactuca sativa

substrate_name: H2O2; L-Ascorbate

enzyme: L-ascorbate peroxidase

substrate: H2O2; L-Ascorbate


organism: Spinacia oleracea


organism: Ovis aries


enzyme_display_name: catechol O-methyltransferase

substrate_name: Rv0187; S-Adenosyl-L-methionine

enzyme: catechol O-methyltransferase

substrate: Rv0187; S-Adenosyl-L-methionine


organism: Streptococcus pyogenes

substrate_name: D-Ribulose 5-phosphate

substrate: D-Ribulose 5-phosphate


organism: Lactococcus lactis subsp. lactis

substrate_name: alpha-D-Glucose

substrate: alpha-D-Glucose


enzyme_display_name: D-sedoheptulose-7-phosphate isomerase

substrate_name: HddA; GmhA

enzyme: D-sedoheptulose-7-phosphate isomerase

substrate: HddA; GmhA


organism: Oryza sativa subsp. japonica

substrate_name: ascorbate

data_origin: experimental

substrate: ascorbate


enzyme_display_name: demethylmacrocin O-methyltransferase

organism: Streptomyces fradiae

substrate_name: demethylmacrocin

enzyme: demethylmacrocin O-methyltransferase

substrate: demethylmacrocin


organism: Aquifex aeolicus (strain VF5)

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


enzyme_display_name: cephaeline 6'-O-methyltransferase

organism: Carapichea ipecacuanha

substrate_name: cephaeline

enzyme: cephaeline 6'-O-methyltransferase

substrate: cephaeline


enzyme_display_name: D-alanine 2-hydroxymethyltransferase

organism: Aminobacter sp

substrate_name: alpha-methyl-L-serine

enzyme: D-alanine 2-hydroxymethyltransferase

substrate: alpha-methyl-L-serine


organism: Ensifer sp


organism: Paracoccus sp


enzyme_display_name: 2-hydroxy-3-oxoadipate synthase

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

enzyme: 2-hydroxy-3-oxoadipate synthase


organism: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

substrate_name: alpha-ketoglutarate

substrate: alpha-ketoglutarate


enzyme_display_name: very-long-chain ceramide synthase

organism: Homo sapiens

substrate_name: sphinganine

assay_conditions_summary: cofactor CoA

enzyme: very-long-chain ceramide synthase

substrate: sphinganine


organism: Cenchrus americanus


organism: Chlamydomonas sp.


enzyme_display_name: aliphatic (R)-hydroxynitrile lyase

organism: Linum usitatissimum

substrate_name: acetone cyanohydrin

enzyme: aliphatic (R)-hydroxynitrile lyase

substrate: acetone cyanohydrin


enzyme_display_name: glutamate racemase

organism: Bacillus anthracis

substrate_name: L-glutamate

assay_conditions_summary: pH 8.2; 298.15 K; cofactor NAD+

proof_lines: mutant R214A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol

proof_lines: mutant R214A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0

enzyme: glutamate racemase

substrate: L-glutamate


substrate_name: D-glutamate

assay_conditions_summary: pH 9.2; 298.15 K; cofactor NAD+

proof_lines: mutant Q86A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.

proof_lines: mutant Q86A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol

substrate: D-glutamate


proof_lines: mutant P99A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.

proof_lines: mutant P99A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol


proof_lines: mutant K106A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol

proof_lines: mutant K106A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0

proof_lines: RacE2 mutant enzyme K106A, pH and temperature not specified in the publication


organism: Burkholderia cenocepacia


enzyme_display_name: adenylate cyclase

organism: Alternaria solani

substrate_name: ATP

enzyme: adenylate cyclase

substrate: ATP


organism: Anabaena sp.

proof_lines: recombinant CyaB1-maltose binding protein fusion


assay_conditions_summary: pH 7.2; 303.15 K; cofactor ATP

proof_lines: forward reaction, 0.1 microM free Ca2+, pH 7.2, 30°C


organism: Bombyx mori


enzyme_display_name: 4-chlorobenzoyl-CoA dehalogenase

organism: Pseudomonas sp.

enzyme: 4-chlorobenzoyl-CoA dehalogenase


enzyme_display_name: imidazoleglycerol-phosphate dehydratase

organism: Arabidopsis thaliana

substrate_name: Unknown

proof_lines: 30°C, pH not specified in the publication

enzyme: imidazoleglycerol-phosphate dehydratase

substrate: Unknown


substrate_name: S-Adenosyl-L-methionine

substrate: S-Adenosyl-L-methionine


organism: Mycobacterium tuberculosis

substrate_name: Glucose 6-phosphate; GDPglucose

substrate: Glucose 6-phosphate; GDPglucose


organism: Streptomyces venezuelae

substrate_name: glucose 6-phosphate

assay_conditions_summary: cofactor ADP

proof_lines: with 2.5 mM ADP-glucose as cosubstrate, pH and temperature not specified in the publication

proof_lines: with 0.5 mM GDP-glucose as cosubstrate, pH and temperature not specified in the publication

substrate: glucose 6-phosphate


organism: Pseudomonas reinekei

assay_conditions_summary: pH 7.5; 298.15 K; cofactor CoA


substrate_name: 4-Chlorobenzoyl-CoA; H2O

substrate: 4-Chlorobenzoyl-CoA; H2O


proof_lines: single turnover, mutant H90Q, pH 7.5, 25°C

proof_lines: multiple turnovers, mutant H90Q, pH 7.5, 25°C


proof_lines: multiple turnovers, wild-type enzyme, pH 7.5, 25°C

proof_lines: single turnover, wild-type enzyme, pH 7.5, 25°C


substrate_name: H2O; 4-Chlorobenzoyl-CoA

substrate: H2O; 4-Chlorobenzoyl-CoA


enzyme_display_name: 5-formyltetrahydrofolate cyclo-ligase

substrate_name: ATP; 5-Formyltetrahydrofolyl-glutamate

enzyme: 5-formyltetrahydrofolate cyclo-ligase

substrate: ATP; 5-Formyltetrahydrofolyl-glutamate


organism: Mus musculus

proof_lines:
pH 6.0, 28°C, recombinant enzyme
recombinant enzyme


organism: Mycoplasma pneumoniae

substrate_name: ATP; 5-Formyltetrahydrofolate

substrate: ATP; 5-Formyltetrahydrofolate


assay_conditions_summary: pH 6; 310.15 K; cofactor ATP


source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0


organism: Mycoplasmoides pneumoniae

proof_lines: mutant enzyme K120A, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C


proof_lines: wild type enzyme, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C


organism: Saccharomyces cerevisiae


organism: Unknown


substrate_name: L-Vinylglycine

substrate: L-Vinylglycine


assay_conditions_summary: cofactor ATP


proof_lines:
below, mutant F64P, pH 7.5, 25°C
mutant F64P, pH 7.5, 25°C


proof_lines:
wild-type enzyme, pH 7.5, 25°C
pH 7.5, 25°C, wild-type enzyme
about, wild-type enzyme, pH 7.5, 25°C


proof_lines: mutant enzyme D81A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: mutant enzyme K120A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: mutant enzyme D151A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: mutant enzyme W153A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C

proof_lines: mutant enzyme W153A, Km above 5 mM, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: mutant enzyme R125A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C

proof_lines: mutant enzyme R125A, Km above 5 mM, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: mutant enzyme K3D/D151K, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: mutant enzyme K3A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C

proof_lines: mutant enzyme K3A, Km above 5 mM, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: mutant enzyme R7A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C

proof_lines: mutant enzyme R7A, Km above 5 mM, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: wild type enzyme, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: mutant enzyme Y152A, in 100 mM MES, pH 6.0, at 37°C


substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


proof_lines: mutant enzyme Y152F, in 100 mM MES, pH 6.0, at 37°C


proof_lines: wild type enzyme, in 100 mM MES, pH 6.0, at 37°C


proof_lines: mutant enzyme Y151A, in 100 mM MES, pH 6.0, at 37°C


organism: Solanum lycopersicum

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


enzyme_display_name: flavone synthase I

organism: Oryza sativa

substrate_name: S-naringenin

assay_conditions_summary: pH 8; 310.15 K; buffer 10 mM Tris/HCl

proof_lines: 37°C, 50 microM FeSO4, 100 mg/ml catalase, 160 microM oxoglutarate, 1 mM ascorbate, 60 microM substrate in 10 mM Tris/HCl buffer, pH 8.0

enzyme: flavone synthase I

substrate: S-naringenin


organism: Petroselinum crispum


enzyme_display_name: tagaturonate reductase

organism: Escherichia coli

substrate_name: NADH

enzyme: tagaturonate reductase

substrate: NADH


organism: Arthrobacter sp.

assay_conditions_summary: pH 7.5; 303.15 K; cofactor CoA


proof_lines:
single turnover
multiple turnovers


organism: Cucurbita maxima


organism: Cucurbita pepo


organism: Dianthus caryophyllus


organism: Diospyros kaki


organism: Vigna radiata


organism: Vigna radiata var. radiata

proof_lines: 8 h after chilling in the presence of 1,4-diazabicyclo(2,2,2) octane


organism: Malus domestica

substrate_name: (S,S)-S-adenosylmethionine

substrate: (S,S)-S-adenosylmethionine


organism: Malus sylvestris


organism: Penicillium citrinum


proof_lines:
at pH 8.5 and 30°C
Km increases from pH 7.5 to 9.5


organism: Homo sapiens

proof_lines:
cytosolic enzyme
mitochondrial enzyme
at 37°C in 100 mM MES, pH 6.0


organism: Lacticaseibacillus casei


organism: Mycoplasmoides pneumoniae


organism: Oryctolagus cuniculus

proof_lines: Km-value is constant over the pH-range 5.0-7.5


organism: Ovis aries


organism: Rattus norvegicus


organism: Saccharomyces cerevisiae

assay_conditions_summary: pH 7; 303.15 K; cofactor ATP


organism: Pichia pastoris

substrate_name: S-Methyl-L-methionine

substrate: S-Methyl-L-methionine


enzyme_display_name: prolyl oligopeptidase

substrate_name: Ala-Pro (Dipeptidyl peptidase activity)

enzyme: prolyl oligopeptidase

substrate: Ala-Pro (Dipeptidyl peptidase activity)


enzyme_display_name: dCTP deaminase

enzyme: dCTP deaminase


enzyme_display_name: prenyl-diphosphate phosphatase

organism: Mus musculus

enzyme: prenyl-diphosphate phosphatase


organism: Escherichia coli O157:H7

substrate_name: histidinol phosphate (in the presence of Mg(2+))


enzyme_display_name: aspartate racemase

organism: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)

substrate_name: L-aspartate

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

enzyme: aspartate racemase

substrate: L-aspartate


organism: Plagiochasma appendiculatum

substrate_name: naringenin

substrate: naringenin


enzyme_display_name: thiosulfate dehydrogenase (quinone)

organism: Acidianus ambivalens

substrate_name: thiosulfate

enzyme: thiosulfate dehydrogenase (quinone)

substrate: thiosulfate


enzyme_display_name: type II protein arginine methyltransferase

proof_lines: isoform PRMT5 in complex with MEP50, presence of 0.001 mM histone H4 peptide, 22°C, pH not specified in the publication

proof_lines: isoform PRMT5, presence of 0.001 mM histone H4 peptide, 22°C, pH not specified in the publication


enzyme_display_name: diaminobutyrate acetyltransferase

organism: Methylophaga alcalica

enzyme: diaminobutyrate acetyltransferase


organism: Methylophaga thalassica


organism: Paenibacillus lautus

assay_conditions_summary: pH 7.4; 303.15 K; cofactor CoA


organism: Trifolium pratense

substrate_name: L-tyrosine

assay_conditions_summary: pH 8; 298.15 K; cofactor CoA

substrate: L-tyrosine


enzyme_display_name: cullin-RING-type E3 NEDD8 transferase

organism: Homo sapiens

substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication

enzyme: cullin-RING-type E3 NEDD8 transferase

substrate: Unknown


organism: Streptomyces venezuelae


proof_lines: pH 8.0, 29°C, addition of the DesIII protein, cosubstrate: 1.0 mM dTDP-3-amino-4,6-dideoxy-alpha-D-glucopyranose

proof_lines: pH 8.0, 29°C, addition of the DesIII protein, cosubstrate: 9.1 mM 10-deoxymethynolide

proof_lines: pH 8.0, 29°C, cosubstrate: 9.1 mM 10-deoxymethynolide


enzyme_display_name: ceramide glucosyltransferase

enzyme: ceramide glucosyltransferase


enzyme_display_name: fructan beta-(2,1)-fructosidase

organism: Lolium perenne

enzyme: fructan beta-(2,1)-fructosidase


enzyme_display_name: 6-aminohexanoate-oligomer endohydrolase

organism: Agromyces sp.

substrate_name: 6-aminohexanoate cyclic oligomer

proof_lines: pH 7.3, 60°C, mutant enzyme D122G/H130Y/D36A/E236Q

enzyme: 6-aminohexanoate-oligomer endohydrolase

substrate: 6-aminohexanoate cyclic oligomer


organism: Escherichia coli

substrate_name: dCTP

proof_lines: pH 6.8, 37°C, recombinant wild-type enzyme

substrate: dCTP


organism: Paramecium bursaria Chlorella virus 1

proof_lines: pH 9.5, 42°C, in presence of 0.1 mM dCTP


enzyme_display_name: o-pyrocatechuate decarboxylase

organism: Fusarium odoratissimum NRRL 54006

substrate_name: catechol

enzyme: o-pyrocatechuate decarboxylase

substrate: catechol


organism: Arabidopsis thaliana


substrate_name: L-cystine

substrate: L-cystine


organism: Tequatrovirus T4

substrate_name: deoxycytidylate

substrate: deoxycytidylate


enzyme_display_name: formate dehydrogenase (cytochrome c-553)

organism: Nitratidesulfovibrio vulgaris

substrate_name: formate

substrate: formate


enzyme: formate dehydrogenase (cytochrome c-553)


enzyme_display_name: sulfiredoxin

organism: Saccharomyces cerevisiae

substrate_name: gamma-S-ATP

enzyme: sulfiredoxin

substrate: gamma-S-ATP


enzyme_display_name: retroviral ribonuclease H

organism: Human immunodeficiency virus 1

substrate_name: RNA-DNA hybrid

proof_lines:
wild type enzyme, at pH 7.8 and 37°C
37°C, wild-type p66/p51

enzyme: retroviral ribonuclease H

substrate: RNA-DNA hybrid


enzyme_display_name: plant seed peroxygenase

organism: Lathyrus oleraceus

substrate_name: linoleic acid hydroperoxide

proof_lines: partially purified enzyme, at pH 7.2 and 25°C

proof_lines: partially purified enzyme, using linoleic acid hydroperoxide as cosubstrate, at pH 7.2 and 25°C

enzyme: plant seed peroxygenase

substrate: linoleic acid hydroperoxide


organism: Solanum lycopersicum

substrate_name: linoleic acid

substrate: linoleic acid


organism: Zea mays


enzyme_display_name: nicotinate dehydrogenase

organism: Clostridium sp.

substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

enzyme: nicotinate dehydrogenase

substrate: NADP+


organism: Neobacillus niacini

substrate_name: nicotinate

substrate: nicotinate


organism: Pseudomonas fluorescens


organism: Nitratidesulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / CCUG 34227 / NCIMB 8303 / VKM B-1760 / Hildenborough)

substrate_name: formate (at pH 9.5 and 30 degrees Celsius)

proof_lines: Measurements have been done with the heterotrimer complex. kcat is 262 sec(-1) with formate as substrate (at pH 7.6).


organism: Acidianus ambivalens

substrate_name: thiosulfate (with ferrocyanide as electron acceptor)

proof_lines: KM values measured using the TQO doxDA complex.


substrate: thiosulfate (with ferrocyanide as electron acceptor)


substrate_name: peroxiredoxin-(S-hydroxy-S-oxocysteine) (in the presence of 1 mM of ATP)

proof_lines: kcat is 0.66 min(-1) with peroxiredoxin-(S-hydroxy-S-oxocysteine) as substrate (in the presence of 1 mM of ATP) and 0.025 min(-1) with ATP as substrate (in the presence of 40 uM of peroxiredoxin-(S-hydroxy-S-oxocysteine)).


organism: Streptomyces niveus

substrate_name: decarbamoylnovobiocin

proof_lines: kcat is 4.1 min(-1) with decarbamoylnovobiocin as substrate.

substrate: decarbamoylnovobiocin


organism: Streptomyces anulatus

proof_lines: kcat is 0.19 min(-1) with 4-MHA as substrate. kcat is 0.44 min(-1) with 4-MHB as substrate. kcat is 0.32 min(-1) with 3-HA as substrate.


organism: Agromyces sp. (strain KY5R)

substrate_name: Ahx cyclic-oligomer

proof_lines: kcat is 11.1 sec(-1) with Ahx cyclic-oligomer as substrate.

substrate: Ahx cyclic-oligomer


organism: Kocuria sp. (strain KY2)

proof_lines: kcat is 11.7 sec(-1) with Ahx cyclic-oligomer as substrate.


organism: Paenarthrobacter ureafaciens

proof_lines: kcat is 6.5 sec(-1) with Ahx cyclic-oligomer as substrate.


organism: Escherichia coli (strain K12)

substrate_name: Unknown


organism: Pusillimonas sp. (ex Stolz et al. 2005)

proof_lines: recombinant enzyme, with phenazine methosulfate, pH 7.0, 30°C


organism: Acidithiobacillus ferrooxidans

substrate_name: thiosulfate

proof_lines:
recombinant enzyme, at pH 2.5 and 50°C
native enzyme, at pH 2.5 and 70°C

substrate: thiosulfate


organism: Desulfovibrio desulfuricans

substrate_name: ferri-Ssc

substrate: ferri-Ssc


organism: Arabidopsis thaliana

substrate_name: peroxiredoxin-(S-hydroxy-S-oxocysteine)

proof_lines: pH 7.5, 30°C, 1 mM ATP, 0.03 mM Srx, saturating substrate concentration

proof_lines: pH 7.5, 30°C, saturating ATP, 0.03 mM Srx, 0.04 mM substrate

substrate: peroxiredoxin-(S-hydroxy-S-oxocysteine)


organism: Homo sapiens

assay_conditions_summary: 303.15 K; cofactor ATP


organism: Tequatrovirus T4

substrate_name: tetrahydrofolate

substrate: tetrahydrofolate


organism: Avena sativa

substrate_name: p-Coumaroyl-CoA

assay_conditions_summary: pH 7

proof_lines: pH 7, temperature not specified in the publication

substrate: p-Coumaroyl-CoA


organism: Methylophaga alcalica

assay_conditions_summary: pH 9.5; 298.15 K; cofactor CoA


organism: Methylophaga thalassica

substrate_name: acetyl-CoA

substrate: acetyl-CoA


organism: Methylotuvimicrobium alcaliphilum


organism: Sus scrofa

substrate_name: UDP-glucose

substrate: UDP-glucose


organism: Gallus gallus


proof_lines:
in absence of c-Fos
in presence of c-Fos
substrate is fluorescent


organism: Raphanus sativus


organism: Rattus norvegicus


substrate_name: oligosaccharide substrate derived from chondroitin 6-sulfate

proof_lines:
liver enzyme
recombinant enzyme


organism: Cichorium intybus


organism: Helianthus tuberosus


organism: Triticum aestivum

proof_lines:
isoenzyme1-FeH w1
isoenzyme1-FeH w2


organism: Streptomyces anulatus


organism: Streptomyces parvulus

substrate_name: 3-hydroxyanthranilic acid

proof_lines: at 30°C, pH not specified in the publication

substrate: 3-hydroxyanthranilic acid


enzyme_display_name: ginsenosidase type IV

organism: Aspergillus sp.

substrate_name: ginsenoside Re

enzyme: ginsenosidase type IV

substrate: ginsenoside Re


organism: Gordonia terrae


enzyme_display_name: scytalidopepsin A

organism: Scytalidium lignicola

substrate_name: Benzyloxycarbonyl-Ala-Phe-Leu-Ala

enzyme: scytalidopepsin A

substrate: Benzyloxycarbonyl-Ala-Phe-Leu-Ala