sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature E161D/S180P/S351G to reconstruct the assayed variant sequence.

proof_lines: recombinant mutant E161D/S180P/S351G, pH 7.5, 80°C

proof_lines: pH 7.5, 80°C, mutant enzyme E161D/S180P/S351G


enzyme_display_name: cellobiose epimerase

organism: Caldicellulosiruptor saccharolyticus

substrate_name: lactose

source_db: brenda

ki_display: Not reported

protein_accession_database: UniProt

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature E161D/N365P to reconstruct the assayed variant sequence.

proof_lines: recombinant mutant E161D/N365P, pH 7.5, 80°C

data_origin: experimental

enzyme: cellobiose epimerase

substrate: lactose

source: brenda


substrate_name: epilactose

mutation_signature: Unknown

kcat_over_km_origin: calculated

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines:
pH 7.5, 50°C, recombinant enzyme
pH 7.5, 50°C

substrate: epilactose


proof_lines:
pH 7.5, 50°C, recombinant enzyme
pH 7.5, 50°C
pH 7.5, 80°C


organism: Pedobacter heparinus

verification_status: corrected

column_label: Table 1 (Biochemical properties of CEs from various bacterial origins) carries one Bacterial strain column, a pH span with Optimum and Stability columns, a Temperature span with Optimum (°C) and Stability (°C) columns, then three substrate spans headed Cellobiose, Lactose and β-(1→4)-Mannobiose, each printing the column heads k_cat (s^-1) | K_m (mM) | k_cat/K_m (s^-1 mM^-1), and a Reference column. The cited cell set is Table 1, row Pedobacter heparinus, Lactose column group: k_cat (s^-1) = 5.43; K_m (mM) = 24.5; k_cat/K_m (s^-1 mM^-1) = 0.222; each of the three cells carries the footnote letter f (30°C).

normalized_value_unit_evidence: kcat: printed Table 1 cell 5.43 under the column head k_cat (s^-1) in the Lactose column group of row Pedobacter heparinus; the column is already per second, so no conversion applies and the paper value is 5.43 s^(-1). Km: printed Table 1 cell 24.5 under the column head K_m (mM) of the same column group; the column is already in mM, so no conversion applies and the paper value is 24.5 mM. kcat/Km: printed Table 1 cell 0.222 under the column head k_cat/K_m (s^-1 mM^-1) of the same column group; the column is already in the canonical unit, so 0.222 x 1 = 0.222 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 1, row Pedobacter heparinus, Lactose column group: kcat = 5.43 s^-1; Km = 24.5 mM; printed kcat/Km = 0.222 s^-1 mM^-1; footnote f states that the kinetic parameters were determined at 30°C.

table_label: Table 1: Biochemical properties of CEs from various bacterial origins (Bioscience, Biotechnology, and Biochemistry 2016, 80(7):1294-1305)

status: corrected


organism: Solanum lycopersicum

substrate_name: S-adenosyl-L-methionine

kcat_over_km_display: Not reported

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

substrate: S-adenosyl-L-methionine


assay_conditions_summary: pH 8.5; buffer containing EPPS pH 8

column_label: PMID 15983009 DOI 10.1093/jxb/eri220 Table 1; row Wild-type; columns Km (uM), Kcat (s^-1), and Kcat/Km (M^-1 s^-1).

row_label: Wild-type LeACS2 / S-adenosyl-L-methionine

table_label: Table 1. Kinetic parameters of wild-type and mutant LeACS2 activity


enzyme_display_name: UDP-glucuronate 4-epimerase

organism: Arabidopsis thaliana

substrate_name: UDP-glucuronate

source_db: uniprot

kcat_display: Not reported

proof_lines: No activity with UDP-Galactose, UDP- Glucose or UDP-Xylose.

enzyme: UDP-glucuronate 4-epimerase

substrate: UDP-glucuronate

source: uniprot


organism: Thermoanaerobacterium saccharolyticum

proof_lines: pH and temperature not specified in the publication


enzyme_display_name: N-acylglucosamine 2-epimerase

organism: Trichormus variabilis

substrate_name: N-acetyl-D-glucosamine

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature K157L to reconstruct the assayed variant sequence.

mutation_type: substitution

proof_lines: pH 7.5, 30°C, recombinant His6-tagged mutant K157L

enzyme: N-acylglucosamine 2-epimerase

substrate: N-acetyl-D-glucosamine


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature K155A to reconstruct the assayed variant sequence.

proof_lines: pH 7.5, 30°C, recombinant His6-tagged mutant K155A


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature K151V to reconstruct the assayed variant sequence.

proof_lines: pH 7.5, 30°C, recombinant His6-tagged mutant K151V


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature K160P to reconstruct the assayed variant sequence.

proof_lines: pH 7.5, 30°C, recombinant His6-tagged mutant K160P


assay_conditions_summary: pH 7; cofactor ATP

proof_lines: pH 7.5, 30°C, recombinant His6-tagged wild-type enzyme

proof_lines: wild type, at pH 7, in the presence of 1 mM ATP

proof_lines: wild type, at pH 8, in the presence of 1 mM ATP


organism: Homo sapiens

substrate_name: N-acyl-D-glucosamine

substrate: N-acyl-D-glucosamine


proof_lines: pH 7.5, 80°C, recombinant wild-type enzyme

proof_lines: recombinant wild-type enzyme, pH 7.5, 80°C


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature A12S to reconstruct the assayed variant sequence.

proof_lines:
pH 7.5, 80°C, recombinant mutant A12S
pH 7.5, 80°C, mutant enzyme A12S


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature A12S/K328I to reconstruct the assayed variant sequence.

proof_lines: pH 7.5, 80°C, recombinant mutant A12S/K328I


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature R5M/A12S/F231L/K328I to reconstruct the assayed variant sequence.

proof_lines: pH 7.5, 80°C, recombinant mutant R5M/A12S/F231L/K328I

proof_lines: pH 7.5, 80°C, mutant enzyme R5M/A12S/F231L/K328I


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature R5M/A12S/I52V/F231L/K328I to reconstruct the assayed variant sequence.

proof_lines: pH 7.5, 80°C, recombinant mutant R5M/A12S/I52V/F231L/K328I

proof_lines: pH 7.5, 80°C, mutant enzyme R5M/A12S/I52V/F231L/K328I


organism: Cellulosilyticum lentocellum

substrate_name: cellobiose

substrate: cellobiose


enzyme_display_name: UDP-N-acetylglucosamine 4-epimerase

organism: Thermotoga maritima

substrate_name: UDP-N-acetyl-alpha-D-galactosamine

source_db: oed

enzyme: UDP-N-acetylglucosamine 4-epimerase

substrate: UDP-N-acetyl-alpha-D-galactosamine

source: oed


substrate_name: UDP-N-acetyl-alpha-D-glucosamine

substrate: UDP-N-acetyl-alpha-D-glucosamine


organism: Nostoc sp.

substrate_name: Unknown

km_display: Not reported

ki_unit: mM

substrate: Unknown


organism: Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019 / LMG 10263 / NCTC 9343 / Onslow / VPI 2553 / EN-2)

proof_lines: kcat is 67.6 sec(-1) with cellobiose as substrate (PubMed:19202279). kcat is 79.5 sec(-1) with lactose as substrate (PubMed:19202279). kcat is 104 sec(-1) with mannobiose as substrate (PubMed:21539815).


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature R5M/I52V/A12S/K328I/F231L/E327D to reconstruct the assayed variant sequence.

proof_lines: pH 7.0, 70°C, mutant enzyme R5M/I52V/A12S/K328I/F231L/E327D

proof_lines: pH 7.0, 70°C, epimerization, mutant enzyme R5M/I52V/A12S/K328I/F231L/E327D


sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.

proof_lines: pH 7.0, 70°C, mutant enzyme R5M/I52V/A12S/K328I/F231L

proof_lines: pH 7.0, 70°C, epimerization, mutant enzyme R5M/I52V/A12S/K328I/F231L


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature A12S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature A12S/K328I to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature R5M/A12S/F231L/K328I to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature R5M/A12S/I52V/F231L/K328I to reconstruct the assayed variant sequence.


organism: Spirochaeta thermophila

verification_status: unverified

status: unverified


organism: Teredinibacter turnerae


organism: uncultured bacterium


substrate_name: mannobiose

substrate: mannobiose


enzyme_display_name: UDP-arabinose 4-epimerase

organism: Hordeum vulgare

substrate_name: UDP-beta-L-arabinopyranose

enzyme: UDP-arabinose 4-epimerase

substrate: UDP-beta-L-arabinopyranose


substrate_name: UDP-alpha-D-xylopyranose

substrate: UDP-alpha-D-xylopyranose


organism: Eubacterium cellulosolvens

proof_lines: kcat is 28.5 sec(-1) for cellobiose. kcat is 32.5 sec(-1) for lactose.


organism: Ruminococcus albus

proof_lines: kcat is 63.8 sec(-1) with cellobiose as substrate. kcat is 52.1 sec(-1) with lactose as substrate.


sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.


organism: Pseudomonas aeruginosa

substrate_name: UDP-N-acetyl-D-glucosamine

sequence_source: multiple

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: kcat is 271 min(-1) with UDP-N-acetylgalactosamine as substrate. kcat is 120 min(-1) with UDP-N-acetylglucosamine as substrate. kcat is 0.188 min(-1) with UDP-galactose as substrate. kcat is 0.124 min(-1) with UDP-glucose as substrate.

source_databases_merged: brenda uniprot

substrate: UDP-N-acetyl-D-glucosamine


substrate_name: UDP-alpha-D-glucose

substrate: UDP-alpha-D-glucose


organism: Herpetosiphon aurantiacus

column_label: Table 1 (Biochemical properties of CEs from various bacterial origins) carries one Bacterial strain column, a pH span with Optimum and Stability columns, a Temperature span with Optimum (°C) and Stability (°C) columns, then three substrate spans headed Cellobiose, Lactose and β-(1→4)-Mannobiose, each printing the column heads k_cat (s^-1) | K_m (mM) | k_cat/K_m (s^-1 mM^-1), and a Reference column. The cited cell set is Table 1, row Herpetosiphon aurantiacus, Lactose column group: k_cat (s^-1) = 14.0; K_m (mM) = 51.9; k_cat/K_m (s^-1 mM^-1) = 0.270; each of the three cells carries the footnote letter f (30°C).

normalized_value_unit_evidence: kcat: printed Table 1 cell 14.0 under the column head k_cat (s^-1) in the Lactose column group of row Herpetosiphon aurantiacus; the column is already per second, so no conversion applies and the paper value is 14.0 s^(-1). Km: printed Table 1 cell 51.9 under the column head K_m (mM) of the same column group; the column is already in mM, so no conversion applies and the paper value is 51.9 mM. kcat/Km: printed Table 1 cell 0.270 under the column head k_cat/K_m (s^-1 mM^-1) of the same column group; the column is already in the canonical unit, so 0.270 x 1 = 0.27 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 1, row Herpetosiphon aurantiacus, Lactose column group: kcat = 14.0 s^-1; Km = 51.9 mM; printed kcat/Km = 0.270 s^-1 mM^-1; footnote f states that the kinetic parameters were determined at 30°C.


organism: Spirosoma linguale

column_label: Table 1 (Biochemical properties of CEs from various bacterial origins) carries one Bacterial strain column, a pH span with Optimum and Stability columns, a Temperature span with Optimum (°C) and Stability (°C) columns, then three substrate spans headed Cellobiose, Lactose and β-(1→4)-Mannobiose, each printing the column heads k_cat (s^-1) | K_m (mM) | k_cat/K_m (s^-1 mM^-1), and a Reference column. The cited cell set is Table 1, row Spirosoma linguale, Lactose column group: k_cat (s^-1) = 92.1; K_m (mM) = 206; k_cat/K_m (s^-1 mM^-1) = 0.447; each of the three cells carries the footnote letter f (30°C).

normalized_value_unit_evidence: kcat: printed Table 1 cell 92.1 under the column head k_cat (s^-1) in the Lactose column group of row Spirosoma linguale; the column is already per second, so no conversion applies and the paper value is 92.1 s^(-1). Km: printed Table 1 cell 206 under the column head K_m (mM) of the same column group; the column is already in mM, so no conversion applies and the paper value is 206.0 mM. kcat/Km: printed Table 1 cell 0.447 under the column head k_cat/K_m (s^-1 mM^-1) of the same column group; the column is already in the canonical unit, so 0.447 x 1 = 0.447 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 1, row Spirosoma linguale, Lactose column group: kcat = 92.1 s^-1; Km = 206 mM; printed kcat/Km = 0.447 s^-1 mM^-1; footnote f states that the kinetic parameters were determined at 30°C.


organism: Arabidopsis thaliana

proof_lines: Equilibrium between UDP-glucuronate and UDP-D-galacturonate established at 1:1.9. No activity with UDP-Galactose, UDP-Glucose, UDP-arabinose, UDP-Xylose, CDP-glucose or GDP-mannose.


organism: Bacillus subtilis

substrate_name: UDP-N-acetylglucosamine

proof_lines: pH and temperature not specified in the publication

substrate: UDP-N-acetylglucosamine


organism: Plesiomonas shigelloides

substrate_name: UDP-GalNAc

proof_lines: kcat is 461 sec(-1) with UDP-GlcNAc. kcat is 1038 sec(-1) with UDP-GalNAc.

substrate: UDP-GalNAc


substrate_name: UDP-D-glucose

assay_conditions_summary: pH 8

substrate: UDP-D-glucose


substrate_name: UDP-glucose

proof_lines: kcat is 19 sec(-1) with UDP-glucose as substrate (PubMed:16644739). kcat is 42 sec(-1) with UDP-galactose as substrate (PubMed:16644739). kcat is 57 sec(-1) with UDP-galactose as substrate (PubMed:16644739). kcat is 66 sec(-1) with UDP-gala

substrate: UDP-glucose


organism: Lathyrus oleraceus

substrate_name: UDP-D-xylose

assay_conditions_summary: pH 8.6; cofactor NAD+

proof_lines: 50 mM Tris-HCl buffer (pH 8.6), 0.1 mM NAD+, 1 mM UDP-sugar and enzyme

substrate: UDP-D-xylose


organism: Trichormus variabilis

assay_conditions_summary: pH 9; cofactor ATP


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature K151V to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature K155A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature K157L to reconstruct the assayed variant sequence.


sequence_variant_note: Mutation signature K160P could not be applied safely because the resolved sequence does not match the expected reference residue(s).


organism: Rhodothermus marinus

proof_lines: kcat is 80.8 sec(-1) with cellobiose as substrate. kcat is 111 sec(-1) with lactose as substrate.


substrate_name: UDP-glucuronate

proof_lines: Equilibrium between UDP-glucuronate and UDP-D-galacturonate established at 1:1.3.

substrate: UDP-glucuronate


organism: Dyadobacter fermentans

column_label: Table 1 row Dyadobacter fermentans; Cellobiose kcat = 240f, Km = 179f, kcat/Km = 1.34f.

raw_value_unit_evidence: Cellobiose columns report kcat 240 s^-1, Km 179 mM, kcat/Km 1.34 s^-1*mM^-1.

row_label: Dyadobacter fermentans; Cellobiose columns

table_label: Table 1. Biochemical properties of CEs from various bacterial origins.


substrate_name: lactose

column_label: Table 1 row Dyadobacter fermentans; Lactose kcat = 44.9f, Km = 95.7f, kcat/Km = 0.469f.

raw_value_unit_evidence: Lactose columns report kcat 44.9 s^-1, Km 95.7 mM, kcat/Km 0.469 s^-1*mM^-1.

row_label: Dyadobacter fermentans; Lactose columns

substrate: lactose


organism: [Eubacterium] cellulosolvens

verification_status: verified

column_label: Table 1; row Eubacterium cellulosolvens; column group Cellobiose; cell kcat (s-1) = 28.5; cell Km (mM) = 11.3; cell kcat/Km (s-1mM-1) = 2.52.

normalized_value_unit_evidence: kcat 28.5 s^-1 = 28.5 s^-1 (already the canonical kcat unit, no conversion). Km 11.3 mM = 11.3 mM (already the canonical Km unit, no conversion). The Cellobiose kcat/Km cell is printed rounded as 2.52 s^-1 mM^-1; the normalized kcat/Km recorded here is this same row's own kcat divided by its own Km, 28.5 / 11.3 = 2.5221238938053094 s^-1 mM^-1, which reproduces the printed cell 2.52 at the precision the table prints.

raw_value_unit_evidence: Table 1. Biochemical properties of CEs from various bacterial origins. Row Eubacterium cellulosolvens, Cellobiose column group: kcat 28.5 s^-1; Km 11.3 mM; kcat/Km 2.52 s^-1 mM^-1.

row_label: Eubacterium cellulosolvens; Cellobiose columns

status: verified


column_label: Table 1; row Eubacterium cellulosolvens; column group Lactose; cell kcat (s-1) = 32.5; cell Km (mM) = 72.0; cell kcat/Km (s-1mM-1) = 0.451.

normalized_value_unit_evidence: kcat 32.5 s^-1 = 32.5 s^-1 (already the canonical kcat unit, no conversion). Km 72.0 mM = 72.0 mM (already the canonical Km unit, no conversion). The Lactose kcat/Km cell is printed rounded as 0.451 s^-1 mM^-1; the normalized kcat/Km recorded here is this same row's own kcat divided by its own Km, 32.5 / 72.0 = 0.4513888888888889 s^-1 mM^-1, which reproduces the printed cell 0.451 at the precision the table prints.

raw_value_unit_evidence: Table 1. Biochemical properties of CEs from various bacterial origins. Row Eubacterium cellulosolvens, Lactose column group: kcat 32.5 s^-1; Km 72.0 mM; kcat/Km 0.451 s^-1 mM^-1.

row_label: Eubacterium cellulosolvens; Lactose columns


organism: Flavobacterium johnsoniae

column_label: The literal Flavobacterium johnsoniae Cellobiose columns bind both current primary components and footnote f binds the assay to 30 C. The current stored efficiency is their deterministic quotient; the independently printed rounded efficiency is retained separately.

normalized_value_unit_evidence: kcat=39.9 s^-1; Km=53.2 mM; current same-row quotient=0.7499999999999999 s^-1 mM^-1; printed rounded efficiency=0.750 s^-1 mM^-1 and is preserved separately

raw_value_unit_evidence: Flavobacterium johnsoniae Cellobiose cells: kcat 39.9f s^-1; Km 53.2f mM; printed kcat/Km 0.750f s^-1 mM^-1

row_label: Flavobacterium johnsoniae / Cellobiose

table_label: Table 1. Biochemical properties of CEs from various bacterial origins


column_label: The literal Flavobacterium johnsoniae Lactose columns bind both current primary components and footnote f binds the assay to 30 C. The current stored efficiency is their deterministic quotient; the independently printed rounded efficiency is retained separately.

normalized_value_unit_evidence: kcat=17.5 s^-1; Km=34.9 mM; current same-row quotient=0.501432664756447 s^-1 mM^-1; printed rounded efficiency=0.501 s^-1 mM^-1 and is preserved separately

raw_value_unit_evidence: Flavobacterium johnsoniae Lactose cells: kcat 17.5f s^-1; Km 34.9f mM; printed kcat/Km 0.501f s^-1 mM^-1

row_label: Flavobacterium johnsoniae / Lactose


organism: Dictyoglomus turgidum

column_label: PMID 27031293 / DOI 10.1080/09168451.2016.1166934, Table 1, row Dictyoglomus turgidum / cellobiose, kcat, Km, and kcat/Km columns for the expected substrate group.

raw_value_unit_evidence: Table 1 Dictyoglomus turgidum / cellobiose: kcat 169 s^-1; Km 75.8 mM; kcat/Km 2.23 s^-1 mM^-1.

row_label: Dictyoglomus turgidum / cellobiose

table_label: Table 1: Biochemical properties of CEs from various bacterial origins


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P0DKY4 and applied mutation signature R377A to reconstruct the assayed variant sequence.

column_label: Table 1 Kinetic values for cellobiose of RaCE and its mutant proteins; row R377A; columns Spec act (U mg-1), kcat (s-1), Km (mM), kcat/Km (s-1 mM-1): R377A 12.1 (43) 26.6 36.8 0.72

normalized_value_unit_evidence: kcat normalized_value=26.6 normalized_unit=s^(-1); Km normalized_value=37.0 normalized_unit=mM; kcat/Km normalized_value=0.7189189189189189 normalized_unit=s^(-1)*mM^(-1)

raw_value_unit_evidence: kcat raw_value=26.6 raw_unit=s^(-1); Km raw_value=36.8 raw_unit=mM; kcat/Km raw_value=0.72 raw_unit=s^(-1)*mM^(-1)


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature W303F to reconstruct the assayed variant sequence.

column_label: Table 1 Kinetic values for cellobiose of RaCE and its mutant proteins; row W303F; columns Spec act (U mg-1), kcat (s-1), Km (mM), kcat/Km (s-1 mM-1): W303F 5.99 (11) 46.4 108 0.43

normalized_value_unit_evidence: kcat normalized_value=46.4 normalized_unit=s^(-1); Km normalized_value=108.0 normalized_unit=mM; kcat/Km normalized_value=0.42962962962962964 normalized_unit=s^(-1)*mM^(-1)

raw_value_unit_evidence: kcat raw_value=46.4 raw_unit=s^(-1); Km raw_value=108.0 raw_unit=mM; kcat/Km raw_value=0.43 raw_unit=s^(-1)*mM^(-1)


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature W55F to reconstruct the assayed variant sequence.

column_label: Table 1 Kinetic values for cellobiose of RaCE and its mutant proteins; row W55F; columns Spec act (U mg-1), kcat (s-1), Km (mM), kcat/Km (s-1 mM-1): W55F 42.0 (84) 215 127 1.69

normalized_value_unit_evidence: kcat normalized_value=215.0 normalized_unit=s^(-1); Km normalized_value=127.0 normalized_unit=mM; kcat/Km normalized_value=1.6929133858267718 normalized_unit=s^(-1)*mM^(-1)

raw_value_unit_evidence: kcat raw_value=215.0 raw_unit=s^(-1); Km raw_value=127.0 raw_unit=mM; kcat/Km raw_value=1.69 raw_unit=s^(-1)*mM^(-1)


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature F114A to reconstruct the assayed variant sequence.

column_label: Table 2 Kinetic values for lactose of RaCE and its mutant proteins; row F114A; columns Spec act (U mg-1), kcat (s-1), Km (mM), kcat/Km (s-1 mM-1): F114A 0.44 (1.6) 1.79 95.2 0.019

normalized_value_unit_evidence: kcat normalized_value=1.79 normalized_unit=s^(-1); kcat/Km normalized_value=0.018842105263157896 normalized_unit=s^(-1)*mM^(-1)

raw_value_unit_evidence: kcat raw_value=1.79 raw_unit=s^(-1); Km raw_value=95.2 raw_unit=mM; kcat/Km raw_value=0.019 raw_unit=s^(-1)*mM^(-1)


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P0DKY4 and applied mutation signature R377A with inferred residue-numbering offset +8 to reconstruct the assayed variant sequence.

column_label: Table 2 Kinetic values for lactose of RaCE and its mutant proteins; row R377A; columns Spec act (U mg-1), kcat (s-1), Km (mM), kcat/Km (s-1 mM-1): R377A 6.60 (23) 20.9 73.3 0.285

normalized_value_unit_evidence: kcat normalized_value=20.9 normalized_unit=s^(-1); kcat/Km normalized_value=0.2863013698630137 normalized_unit=s^(-1)*mM^(-1)

raw_value_unit_evidence: kcat raw_value=20.9 raw_unit=s^(-1); Km raw_value=73.3 raw_unit=mM; kcat/Km raw_value=0.285 raw_unit=s^(-1)*mM^(-1)


column_label: Table 2 Kinetic values for lactose of RaCE and its mutant proteins; row W303F; columns Spec act (U mg-1), kcat (s-1), Km (mM), kcat/Km (s-1 mM-1): W303F 0.53 (1.9) 4.04 145 0.028

normalized_value_unit_evidence: kcat normalized_value=4.04 normalized_unit=s^(-1); Km normalized_value=145.0 normalized_unit=mM; kcat/Km normalized_value=0.027862068965517243 normalized_unit=s^(-1)*mM^(-1)

raw_value_unit_evidence: kcat raw_value=4.04 raw_unit=s^(-1); Km raw_value=145.0 raw_unit=mM; kcat/Km raw_value=0.028 raw_unit=s^(-1)*mM^(-1)


column_label: Table 2 Kinetic values for lactose of RaCE and its mutant proteins; row W55F; columns Spec act (U mg-1), kcat (s-1), Km (mM), kcat/Km (s-1 mM-1): W55F 22.7 (80) 78.3 72.3 1.08

normalized_value_unit_evidence: kcat normalized_value=78.3 normalized_unit=s^(-1); kcat/Km normalized_value=1.0875 normalized_unit=s^(-1)*mM^(-1)

raw_value_unit_evidence: kcat raw_value=78.3 raw_unit=s^(-1); Km raw_value=72.3 raw_unit=mM; kcat/Km raw_value=1.08 raw_unit=s^(-1)*mM^(-1)


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P0DKY4 and applied mutation signature F114A to reconstruct the assayed variant sequence.

column_label: Table 1: Kinetic values for cellobiose of RaCE and its mutant proteins (Biotechnology Letters 2009, 31(7): 1065-1071), printed column heads Mutation | Spec act (U mg-1) | kcat (s-1) | Km (mM) | kcat/Km (s-1 mM-1). Manuscript page 16 (PDF page 17). Table 1, mutation row F114A; columns kcat (s-1), Km (mM) and kcat/Km (s-1 mM-1); kcat cell = 4.64; Km cell = 55.6; kcat/Km cell = 0.08. captured primary segment: cellobiose | F114A RaCE, the site-directed F114A single-substitution mutant of recombinant Ruminococcus albus cellobiose 2-epimerase expressed in Escherichia coli BL21 (DE3) | Table 1 kcat 4.64 s-1 | Table 1 Km 55.6 mM | Table 1 kcat/Km printed cell 0.08 under the column head kcat/Km (s-1 mM-1)

normalized_value_unit_evidence: kcat: printed Table 1 kcat cell 4.64 under the column head kcat (s-1); the printed turnover column is already per second, so no unit conversion applies and the value is 4.64 s^(-1); the export stores 4.64 s^(-1). Km: printed Table 1 Km cell 55.6 under the column head Km (mM); the printed Michaelis column is already in millimolar, so no unit conversion applies and the value is 55.6 mM; the export stores 56.0 mM. kcat/Km: printed Table 1 kcat/Km cell 0.08 under the column head kcat/Km (s-1 mM-1); the printed efficiency column is already per second per millimolar, so no unit conversion applies and the value is 0.08 s^(-1)*mM^(-1); the export stores 0.08285714285714285 s^(-1)*mM^(-1).

raw_value_unit_evidence: Manuscript page 16 (PDF page 17), Table 1, mutation row F114A: kcat = 4.64 s-1; Km = 55.6 mM; kcat/Km = 0.08 under the printed column head kcat/Km (s-1 mM-1).

row_label: Table 1, mutation row F114A


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P0DKY4 and applied mutation signature M106T to reconstruct the assayed variant sequence.

column_label: Table 1: Kinetic values for cellobiose of RaCE and its mutant proteins (Biotechnology Letters 2009, 31(7): 1065-1071), printed column heads Mutation | Spec act (U mg-1) | kcat (s-1) | Km (mM) | kcat/Km (s-1 mM-1). Manuscript page 16 (PDF page 17). Table 1, mutation row M106T; columns kcat (s-1), Km (mM) and kcat/Km (s-1 mM-1); kcat cell = 29.7; Km cell = 128; kcat/Km cell = 0.23. captured primary segment: cellobiose | M106T RaCE, the site-directed M106T single-substitution mutant of recombinant Ruminococcus albus cellobiose 2-epimerase expressed in Escherichia coli BL21 (DE3) | Table 1 kcat 29.7 s-1 | Table 1 Km 128 mM | Table 1 kcat/Km printed cell 0.23 under the column head kcat/Km (s-1 mM-1)

normalized_value_unit_evidence: kcat: printed Table 1 kcat cell 29.7 under the column head kcat (s-1); the printed turnover column is already per second, so no unit conversion applies and the value is 29.7 s^(-1); the export stores 29.7 s^(-1). Km: printed Table 1 Km cell 128 under the column head Km (mM); the printed Michaelis column is already in millimolar, so no unit conversion applies and the value is 128.0 mM; the export stores 128.0 mM. kcat/Km: printed Table 1 kcat/Km cell 0.23 under the column head kcat/Km (s-1 mM-1); the printed efficiency column is already per second per millimolar, so no unit conversion applies and the value is 0.23 s^(-1)*mM^(-1); the export stores 0.23203125 s^(-1)*mM^(-1).

raw_value_unit_evidence: Manuscript page 16 (PDF page 17), Table 1, mutation row M106T: kcat = 29.7 s-1; Km = 128 mM; kcat/Km = 0.23 under the printed column head kcat/Km (s-1 mM-1).

row_label: Table 1, mutation row M106T


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P0DKY4 and applied mutation signature M179T to reconstruct the assayed variant sequence.

column_label: Table 1: Kinetic values for cellobiose of RaCE and its mutant proteins (Biotechnology Letters 2009, 31(7): 1065-1071), printed column heads Mutation | Spec act (U mg-1) | kcat (s-1) | Km (mM) | kcat/Km (s-1 mM-1). Manuscript page 16 (PDF page 17). Table 1, mutation row M179T; columns kcat (s-1), Km (mM) and kcat/Km (s-1 mM-1); kcat cell = 36.9; Km cell = 146; kcat/Km cell = 0.25. captured primary segment: cellobiose | M179T RaCE, the site-directed M179T single-substitution mutant of recombinant Ruminococcus albus cellobiose 2-epimerase expressed in Escherichia coli BL21 (DE3) | Table 1 kcat 36.9 s-1 | Table 1 Km 146 mM | Table 1 kcat/Km printed cell 0.25 under the column head kcat/Km (s-1 mM-1)

normalized_value_unit_evidence: kcat: printed Table 1 kcat cell 36.9 under the column head kcat (s-1); the printed turnover column is already per second, so no unit conversion applies and the value is 36.9 s^(-1); the export stores 36.9 s^(-1). Km: printed Table 1 Km cell 146 under the column head Km (mM); the printed Michaelis column is already in millimolar, so no unit conversion applies and the value is 146.0 mM; the export stores 146.0 mM. kcat/Km: printed Table 1 kcat/Km cell 0.25 under the column head kcat/Km (s-1 mM-1); the printed efficiency column is already per second per millimolar, so no unit conversion applies and the value is 0.25 s^(-1)*mM^(-1); the export stores 0.25273972602739725 s^(-1)*mM^(-1).

raw_value_unit_evidence: Manuscript page 16 (PDF page 17), Table 1, mutation row M179T: kcat = 36.9 s-1; Km = 146 mM; kcat/Km = 0.25 under the printed column head kcat/Km (s-1 mM-1).

row_label: Table 1, mutation row M179T


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P0DKY4 and applied mutation signature M212T to reconstruct the assayed variant sequence.

column_label: Table 1: Kinetic values for cellobiose of RaCE and its mutant proteins (Biotechnology Letters 2009, 31(7): 1065-1071), printed column heads Mutation | Spec act (U mg-1) | kcat (s-1) | Km (mM) | kcat/Km (s-1 mM-1). Manuscript page 16 (PDF page 17). Table 1, mutation row M212T; columns kcat (s-1), Km (mM) and kcat/Km (s-1 mM-1); kcat cell = 31.5; Km cell = 20.1; kcat/Km cell = 1.57. captured primary segment: cellobiose | M212T RaCE, the site-directed M212T single-substitution mutant of recombinant Ruminococcus albus cellobiose 2-epimerase expressed in Escherichia coli BL21 (DE3) | Table 1 kcat 31.5 s-1 | Table 1 Km 20.1 mM | Table 1 kcat/Km printed cell 1.57 under the column head kcat/Km (s-1 mM-1)

normalized_value_unit_evidence: kcat: printed Table 1 kcat cell 31.5 under the column head kcat (s-1); the printed turnover column is already per second, so no unit conversion applies and the value is 31.5 s^(-1); the export stores 31.5 s^(-1). Km: printed Table 1 Km cell 20.1 under the column head Km (mM); the printed Michaelis column is already in millimolar, so no unit conversion applies and the value is 20.1 mM; the export stores 20.0 mM. kcat/Km: printed Table 1 kcat/Km cell 1.57 under the column head kcat/Km (s-1 mM-1); the printed efficiency column is already per second per millimolar, so no unit conversion applies and the value is 1.57 s^(-1)*mM^(-1); the export stores 1.575 s^(-1)*mM^(-1).

raw_value_unit_evidence: Manuscript page 16 (PDF page 17), Table 1, mutation row M212T: kcat = 31.5 s-1; Km = 20.1 mM; kcat/Km = 1.57 under the printed column head kcat/Km (s-1 mM-1).

row_label: Table 1, mutation row M212T


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature M270T to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P0DKY4 and applied mutation signature M378T to reconstruct the assayed variant sequence.

column_label: Table 1: Kinetic values for cellobiose of RaCE and its mutant proteins (Biotechnology Letters 2009, 31(7): 1065-1071), printed column heads Mutation | Spec act (U mg-1) | kcat (s-1) | Km (mM) | kcat/Km (s-1 mM-1). Manuscript page 16 (PDF page 17). Table 1, mutation row M378T; columns kcat (s-1), Km (mM) and kcat/Km (s-1 mM-1); kcat cell = 5.16; Km cell = 83.7; kcat/Km cell = 0.06. captured primary segment: cellobiose | M378T RaCE, the site-directed M378T single-substitution mutant of recombinant Ruminococcus albus cellobiose 2-epimerase expressed in Escherichia coli BL21 (DE3) | Table 1 kcat 5.16 s-1 | Table 1 Km 83.7 mM | Table 1 kcat/Km printed cell 0.06 under the column head kcat/Km (s-1 mM-1)

normalized_value_unit_evidence: kcat: printed Table 1 kcat cell 5.16 under the column head kcat (s-1); the printed turnover column is already per second, so no unit conversion applies and the value is 5.16 s^(-1); the export stores 5.16 s^(-1). Km: printed Table 1 Km cell 83.7 under the column head Km (mM); the printed Michaelis column is already in millimolar, so no unit conversion applies and the value is 83.7 mM; the export stores 84.0 mM. kcat/Km: printed Table 1 kcat/Km cell 0.06 under the column head kcat/Km (s-1 mM-1); the printed efficiency column is already per second per millimolar, so no unit conversion applies and the value is 0.06 s^(-1)*mM^(-1); the export stores 0.06142857142857143 s^(-1)*mM^(-1).

raw_value_unit_evidence: Manuscript page 16 (PDF page 17), Table 1, mutation row M378T: kcat = 5.16 s-1; Km = 83.7 mM; kcat/Km = 0.06 under the printed column head kcat/Km (s-1 mM-1).

row_label: Table 1, mutation row M378T


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P0DKY4 and applied mutation signature M380T to reconstruct the assayed variant sequence.

column_label: Table 1: Kinetic values for cellobiose of RaCE and its mutant proteins (Biotechnology Letters 2009, 31(7): 1065-1071), printed column heads Mutation | Spec act (U mg-1) | kcat (s-1) | Km (mM) | kcat/Km (s-1 mM-1). Manuscript page 16 (PDF page 17). Table 1, mutation row M380T; columns kcat (s-1), Km (mM) and kcat/Km (s-1 mM-1); kcat cell = 40.0; Km cell = 34.3; kcat/Km cell = 1.17. captured primary segment: cellobiose | M380T RaCE, the site-directed M380T single-substitution mutant of recombinant Ruminococcus albus cellobiose 2-epimerase expressed in Escherichia coli BL21 (DE3) | Table 1 kcat 40.0 s-1 | Table 1 Km 34.3 mM | Table 1 kcat/Km printed cell 1.17 under the column head kcat/Km (s-1 mM-1)

normalized_value_unit_evidence: kcat: printed Table 1 kcat cell 40.0 under the column head kcat (s-1); the printed turnover column is already per second, so no unit conversion applies and the value is 40.0 s^(-1); the export stores 40.0 s^(-1). Km: printed Table 1 Km cell 34.3 under the column head Km (mM); the printed Michaelis column is already in millimolar, so no unit conversion applies and the value is 34.3 mM; the export stores 34.0 mM. kcat/Km: printed Table 1 kcat/Km cell 1.17 under the column head kcat/Km (s-1 mM-1); the printed efficiency column is already per second per millimolar, so no unit conversion applies and the value is 1.17 s^(-1)*mM^(-1); the export stores 1.1764705882352942 s^(-1)*mM^(-1).

raw_value_unit_evidence: Manuscript page 16 (PDF page 17), Table 1, mutation row M380T: kcat = 40.0 s-1; Km = 34.3 mM; kcat/Km = 1.17 under the printed column head kcat/Km (s-1 mM-1).

row_label: Table 1, mutation row M380T


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P0DKY4 and applied mutation signature M62T to reconstruct the assayed variant sequence.

column_label: Table 1: Kinetic values for cellobiose of RaCE and its mutant proteins (Biotechnology Letters 2009, 31(7): 1065-1071), printed column heads Mutation | Spec act (U mg-1) | kcat (s-1) | Km (mM) | kcat/Km (s-1 mM-1). Manuscript page 16 (PDF page 17). Table 1, mutation row M62T; columns kcat (s-1), Km (mM) and kcat/Km (s-1 mM-1); kcat cell = 30.3; Km cell = 53.8; kcat/Km cell = 0.58. captured primary segment: cellobiose | M62T RaCE, the site-directed M62T single-substitution mutant of recombinant Ruminococcus albus cellobiose 2-epimerase expressed in Escherichia coli BL21 (DE3) | Table 1 kcat 30.3 s-1 | Table 1 Km 53.8 mM | Table 1 kcat/Km printed cell 0.58 under the column head kcat/Km (s-1 mM-1)

normalized_value_unit_evidence: kcat: printed Table 1 kcat cell 30.3 under the column head kcat (s-1); the printed turnover column is already per second, so no unit conversion applies and the value is 30.3 s^(-1); the export stores 30.3 s^(-1). Km: printed Table 1 Km cell 53.8 under the column head Km (mM); the printed Michaelis column is already in millimolar, so no unit conversion applies and the value is 53.8 mM; the export stores 54.0 mM. kcat/Km: printed Table 1 kcat/Km cell 0.58 under the column head kcat/Km (s-1 mM-1); the printed efficiency column is already per second per millimolar, so no unit conversion applies and the value is 0.58 s^(-1)*mM^(-1); the export stores 0.5611111111111111 s^(-1)*mM^(-1).

raw_value_unit_evidence: Manuscript page 16 (PDF page 17), Table 1, mutation row M62T: kcat = 30.3 s-1; Km = 53.8 mM; kcat/Km = 0.58 under the printed column head kcat/Km (s-1 mM-1).

row_label: Table 1, mutation row M62T


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P0DKY4 and applied mutation signature M95T to reconstruct the assayed variant sequence.

column_label: Table 1: Kinetic values for cellobiose of RaCE and its mutant proteins (Biotechnology Letters 2009, 31(7): 1065-1071), printed column heads Mutation | Spec act (U mg-1) | kcat (s-1) | Km (mM) | kcat/Km (s-1 mM-1). Manuscript page 16 (PDF page 17). Table 1, mutation row M95T; columns kcat (s-1), Km (mM) and kcat/Km (s-1 mM-1); kcat cell = 33.4; Km cell = 56.2; kcat/Km cell = 0.61. captured primary segment: cellobiose | M95T RaCE, the site-directed M95T single-substitution mutant of recombinant Ruminococcus albus cellobiose 2-epimerase expressed in Escherichia coli BL21 (DE3) | Table 1 kcat 33.4 s-1 | Table 1 Km 56.2 mM | Table 1 kcat/Km printed cell 0.61 under the column head kcat/Km (s-1 mM-1)

normalized_value_unit_evidence: kcat: printed Table 1 kcat cell 33.4 under the column head kcat (s-1); the printed turnover column is already per second, so no unit conversion applies and the value is 33.4 s^(-1); the export stores 33.3 s^(-1). Km: printed Table 1 Km cell 56.2 under the column head Km (mM); the printed Michaelis column is already in millimolar, so no unit conversion applies and the value is 56.2 mM; the export stores 56.0 mM. kcat/Km: printed Table 1 kcat/Km cell 0.61 under the column head kcat/Km (s-1 mM-1); the printed efficiency column is already per second per millimolar, so no unit conversion applies and the value is 0.61 s^(-1)*mM^(-1); the export stores 0.5946428571428571 s^(-1)*mM^(-1).

raw_value_unit_evidence: Manuscript page 16 (PDF page 17), Table 1, mutation row M95T: kcat = 33.4 s-1; Km = 56.2 mM; kcat/Km = 0.61 under the printed column head kcat/Km (s-1 mM-1).

row_label: Table 1, mutation row M95T


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature M96T to reconstruct the assayed variant sequence.

column_label: Table 1 Kinetic values for cellobiose of RaCE and its mutant proteins; row M96T; columns Spec act (U mg-1), kcat (s-1), Km (mM), kcat/Km (s-1 mM-1): M96T 8.73 (17) 43.8 121 0.37

raw_value_unit_evidence: kcat raw_value=43.8 raw_unit=s^(-1); Km raw_value=121.0 raw_unit=mM; kcat/Km raw_value=0.37 raw_unit=s^(-1)*mM^(-1)


organism: Salmonella typhi

substrate_name: UDP-GlcNAcA

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: kcat is 56.8 min(-1) with UDP-GlcNAcA as substrate. kcat is 39.1 min(-1) with UDP-GalNAcA as substrate. kcat is 14.2 min(-1) with UDP-GlcNAc as substrate. kcat is 52.6 min(-1) with UDP-GalNAc as substrate.

substrate: UDP-GlcNAcA


substrate_name: UDP-GalNAcA

substrate: UDP-GalNAcA


substrate_name: UDP-GlcNAc

substrate: UDP-GlcNAc


organism: Bacteroides fragilis

substrate_name: cellobiose

column_label: Table 3 rBfCE Cellobiose column: specific activity 61.8 U/mg; Km 3.75 mM; kcat 67.6 s^-1; kcat/Km 18.0 s^-1 mM^-1.

normalized_value_unit_evidence: Normalized values: kcat=67.6 s^-1; Km=3.75 mM; independently printed kcat/Km=18.0 s^-1 mM^-1; current values are kcat=67.6 s^-1, Km=4.35 mM, and quotient-derived kcat/Km=15.540229885057471 s^-1 mM^-1.

row_label: rBfCE

table_label: Table 3. Kinetic Parameters of rBfCE and rRaCE toward Cellobiose and Lactose

substrate: cellobiose


column_label: J-STAGE PDF Table 3 rBfCE lactose column gives Km=6.56 mM, kcat=79.5 s^-1, and kcat/Km=12.1 s^-1*mM^-1; exported kcat/Km is 12.11890243902439.

normalized_value_unit_evidence: Km normalized_value=6.56 mM; kcat normalized_value=79.5 s^-1; kcat/Km normalized_value=12.1 s^-1*mM^-1

raw_value_unit_evidence: Km raw_value=6.56 raw_unit=mM; kcat raw_value=79.5 raw_unit=s^-1; kcat/Km raw_value=12.1 raw_unit=s^-1*mM^-1

row_label: rBfCE lactose column; Km, kcat, and kcat/Km rows

table_label: Table 3 Kinetic Parameters of rBfCE and rRaCE toward Cellobiose and Lactose


organism: Pedobacter heparinus

column_label: PMID 27031293 / DOI 10.1080/09168451.2016.1166934, Table 1, row Pedobacter heparinus / cellobiose, kcat, Km, and kcat/Km columns for the expected substrate group.

raw_value_unit_evidence: Table 1 Pedobacter heparinus / cellobiose: kcat 7.02 s^-1; Km 29.6 mM; kcat/Km 0.237 s^-1 mM^-1.

row_label: Pedobacter heparinus / cellobiose


column_label: PMID 27031293 / DOI 10.1080/09168451.2016.1166934, Table 1, row Pedobacter heparinus / lactose, kcat, Km, and kcat/Km columns for the expected substrate group.

raw_value_unit_evidence: Table 1 Pedobacter heparinus / lactose: kcat 5.43 s^-1; Km 24.5 mM; kcat/Km 0.222 s^-1 mM^-1.

row_label: Pedobacter heparinus / lactose


organism: Saccharophagus degradans

column_label: Table 1; row Saccharophagus degradans; column group Cellobiose; cell kcat (s-1) = 26.1f; cell Km (mM) = 22.6f; cell kcat/Km (s-1mM-1) = 1.15f.

normalized_value_unit_evidence: kcat 26.1f s^-1 = 26.1 s^-1 (already the canonical kcat unit, no conversion). Km 22.6f mM = 22.6 mM (already the canonical Km unit, no conversion). The Cellobiose kcat/Km cell is printed rounded as 1.15f s^-1 mM^-1; the normalized kcat/Km recorded here is this same row's own kcat divided by its own Km, 26.1 / 22.6 = 1.154867256637168 s^-1 mM^-1, which reproduces the printed cell 1.15 at the precision the table prints.

raw_value_unit_evidence: Table 1. Biochemical properties of CEs from various bacterial origins. Row Saccharophagus degradans, Cellobiose column group: kcat 26.1f s^-1; Km 22.6f mM; kcat/Km 1.15f s^-1 mM^-1.

row_label: Saccharophagus degradans; Cellobiose columns


column_label: Table 1; row Saccharophagus degradans; column group Lactose; cell kcat (s-1) = 7.82f; cell Km (mM) = 29.2f; cell kcat/Km (s-1mM-1) = 0.268f.

normalized_value_unit_evidence: kcat 7.82f s^-1 = 7.82 s^-1 (already the canonical kcat unit, no conversion). Km 29.2f mM = 29.2 mM (already the canonical Km unit, no conversion). The Lactose kcat/Km cell is printed rounded as 0.268f s^-1 mM^-1; the normalized kcat/Km recorded here is this same row's own kcat divided by its own Km, 7.82 / 29.2 = 0.26780821917808223 s^-1 mM^-1, which reproduces the printed cell 0.268 at the precision the table prints.

raw_value_unit_evidence: Table 1. Biochemical properties of CEs from various bacterial origins. Row Saccharophagus degradans, Lactose column group: kcat 7.82f s^-1; Km 29.2f mM; kcat/Km 0.268f s^-1 mM^-1.

row_label: Saccharophagus degradans; Lactose columns


column_label: PMID 27031293 / DOI 10.1080/09168451.2016.1166934, Table 1, row Spirosoma linguale / cellobiose, kcat, Km, and kcat/Km columns for the expected substrate group.

raw_value_unit_evidence: Table 1 Spirosoma linguale / cellobiose: kcat 222 s^-1; Km 104 mM; kcat/Km 2.13 s^-1 mM^-1.

row_label: Spirosoma linguale / cellobiose


column_label: The literal Spirosoma linguale Lactose columns bind both current primary components and the preceding superscript-only line binds their assay to footnote f/30 C. The current stored efficiency is their deterministic quotient; the independently printed rounded efficiency is retained separately.

normalized_value_unit_evidence: kcat=92.1 s^-1; Km=206 mM; current same-row quotient=0.4470873786407767 s^-1 mM^-1; printed rounded efficiency=0.447 s^-1 mM^-1 and is preserved separately

raw_value_unit_evidence: Spirosoma linguale Lactose cells: kcat 92.1 with footnote f in s^-1; Km 206 with footnote f in mM; printed kcat/Km 0.447 with footnote f in s^-1 mM^-1

row_label: Spirosoma linguale / Lactose


organism: Caldicellulosiruptor obsidiansis


organism: Caldicellulosiruptor morganii

assay_conditions_summary: pH 7


enzyme_display_name: CDP-paratose 2-epimerase

organism: Salmonella enterica subsp. enterica serovar Enteritidis

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

enzyme: CDP-paratose 2-epimerase


organism: Spirochaeta thermophila


organism: Caldicellulosiruptor saccharolyticus

substrate_name: D-glucose

assay_conditions_summary: pH 7.5; buffer 50 mM PIPES

substrate: D-glucose


organism: Ruminococcus albus

column_label: J-STAGE PDF Table 3 rRaCE cellobiose column gives Km=13.8 mM, kcat=63.8 s^-1, and kcat/Km=4.62 s^-1*mM^-1; exported kcat/Km is 4.623188405797101.

normalized_value_unit_evidence: Km normalized_value=13.8 mM; kcat normalized_value=63.8 s^-1; kcat/Km normalized_value=4.62 s^-1*mM^-1

raw_value_unit_evidence: Km raw_value=13.8 raw_unit=mM; kcat raw_value=63.8 raw_unit=s^-1; kcat/Km raw_value=4.62 raw_unit=s^-1*mM^-1

row_label: rRaCE cellobiose column; Km, kcat, and kcat/Km rows


column_label: J-STAGE PDF Table 3 rRaCE lactose column gives Km=33.0 mM, kcat=52.1 s^-1, and kcat/Km=1.58 s^-1*mM^-1; exported kcat/Km is 1.5787878787878789.

normalized_value_unit_evidence: Km normalized_value=33.0 mM; kcat normalized_value=52.1 s^-1; kcat/Km normalized_value=1.58 s^-1*mM^-1

raw_value_unit_evidence: Km raw_value=33.0 raw_unit=mM; kcat raw_value=52.1 raw_unit=s^-1; kcat/Km raw_value=1.58 raw_unit=s^-1*mM^-1

row_label: rRaCE lactose column; Km, kcat, and kcat/Km rows


column_label: Table 1 R. marinus cellobiose row reports kcat 80.8, Km 27.2, and kcat/Km 2.97.

normalized_value_unit_evidence: kcat normalized_value=80.8 normalized_unit=s^-1; Km normalized_value=27.2 normalized_unit=mM; kcat/Km normalized_value=2.97 normalized_unit=s^-1 mM^-1

raw_value_unit_evidence: kcat raw_value=80.8 raw_unit=s^-1; Km raw_value=27.2 raw_unit=mM; kcat/Km raw_value=2.97 raw_unit=s^-1 mM^-1

row_label: R. marinus / Cellobiose

table_label: J-STAGE PDF Table 1. Kinetic Parameters of CE for Cellobiose and Lactose


assay_conditions_summary: pH 6.3; 333.15 K; buffer 1 mL reactions containing 30 mM sodium phosphate

column_label: Table 1 R. marinus Lactose column: kcat 111 s^-1; Km 28.8 mM; kcat/Km 3.85 s^-1 mM^-1.

normalized_value_unit_evidence: Normalized values: kcat=111 s^-1; Km=28.8 mM; independently printed kcat/Km=3.85 s^-1 mM^-1.

row_label: R. marinus

table_label: Table 1. Kinetic Parameters of CE for Cellobiose and Lactose


organism: Herpetosiphon aurantiacus

column_label: Table 1 row Herpetosiphon aurantiacus; Cellobiose kcat = 18.7, Km = 28.2, kcat/Km = 0.663.

raw_value_unit_evidence: Cellobiose columns report kcat 18.7 s^-1, Km 28.2 mM, kcat/Km 0.663 s^-1*mM^-1.

row_label: Herpetosiphon aurantiacus; Cellobiose columns


column_label: Table 1 row Bacteroides fragilis; beta-(1->4)-Mannobiose kcat = 104f, Km = 5.51f, kcat/Km = 18.9f.

raw_value_unit_evidence: beta-(1->4)-Mannobiose columns report kcat 104 s^-1, Km 5.51 mM, kcat/Km 18.9 s^-1*mM^-1.

row_label: Bacteroides fragilis; beta-(1->4)-Mannobiose columns


organism: Arabidopsis thaliana


organism: Homo sapiens

substrate_name: UDP-galactose (at 37 degrees Celsius and pH 8.8)

sequence_variant_note: Resolved canonical sequence from source_record and applied mutation signature V94M to reconstruct the assayed variant sequence.


organism: Thermotoga maritima

substrate_name: UDP-alpha-D-galactose

substrate: UDP-alpha-D-galactose


organism: Streptococcus pneumoniae

substrate_name: UDP-D-glucuronate

column_label: Figure 4D and Results prose; UDP-GlcA concentration dependence; metric apparent Km

normalized_value_unit_evidence: normalized: Km=0.24 mM

raw_value_unit_evidence: paper text: K m of 0.24 mM from Lineweaver-Burk plot of UDPGLE activity on UDP-GlcA

row_label: Cap1J UDPGLE / UDP-GlcA

table_label: Figure 4D plus Results prose

substrate: UDP-D-glucuronate


organism: Pisum sativum

substrate_name: UDP-galactose

column_label: Abstract reports apparent Km values of 0.31, 0.29, 0.16 and 0.15 mM for UDP-Glc, UDP-Gal, UDP-Ara and UDP-Xyl respectively.

raw_value_unit_evidence: Km raw_value=0.29 mM raw_unit=mM

row_label: recombinant Pisum sativum PsUGE1 / UDP-Gal

table_label: PubMed XML abstract PMID 19754426

substrate: UDP-galactose


organism: Nostoc punctiforme

substrate_name: N-acetyl-D-glucosamine

substrate: N-acetyl-D-glucosamine


enzyme_display_name: UDP-glucosamine 4-epimerase

organism: Bos taurus

substrate_name: UDPgalactose

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

enzyme: UDP-glucosamine 4-epimerase

substrate: UDPgalactose


organism: Rattus norvegicus

substrate_name: Unknown

substrate: Unknown


organism: Escherichia coli

substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


organism: Rhodothermus marinus


enzyme_display_name: chondroitin-glucuronate 5-epimerase

substrate_name: D-glucuronate

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature N648S to reconstruct the assayed variant sequence.

enzyme: chondroitin-glucuronate 5-epimerase

substrate: D-glucuronate


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature N336S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature N183S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature N642S to reconstruct the assayed variant sequence.


proof_lines: ph 5.5, 37°C, recombinant wild-type enzyme


enzyme_display_name: GDP-mannose 3,5-epimerase

organism: Auxenochlorella pyrenoidosa

substrate_name: GDP-alpha-D-mannose

enzyme: GDP-mannose 3,5-epimerase

substrate: GDP-alpha-D-mannose


column_label: PMID 27031293 / DOI 10.1080/09168451.2016.1166934, Table 1, row Ruminococcus albus / beta-(1->4)-mannobiose, kcat, Km, and kcat/Km columns for the expected substrate group.

raw_value_unit_evidence: Table 1 Ruminococcus albus / beta-(1->4)-mannobiose: kcat 201 s^-1; Km 8.74 mM; kcat/Km 23.0 s^-1 mM^-1.


proof_lines: UDP-N-acetyl-D-glucosamine, liver enzyme of tumor-bearing animals


organism: Sinorhizobium meliloti

substrate_name: UDP-D-xylopyranose

substrate: UDP-D-xylopyranose


organism: Neisseria meningitidis serogroup A / serotype 4A

substrate_name: UDP-ManNGc

proof_lines: kcat is 31 sec(-1) with UDP-GlcNAc as substrate. kcat is 52 sec(-1) with UDP-ManNAc as substrate. kcat is 4.2 sec(-1) with UDP-ManNGc as substrate. kcat is 7.8 sec(-1) with UDP-ManNPr as substrate. kcat is 6.7 sec(-1) with rUDP-ManNAc as su

substrate: UDP-ManNGc


substrate_name: D-glucuronosyl residues

substrate: D-glucuronosyl residues


enzyme_display_name: ADP-glyceromanno-heptose 6-epimerase

organism: Escherichia coli (strain K12)

substrate_name: ADP-heptose

assay_conditions_summary: cofactor ADP

enzyme: ADP-glyceromanno-heptose 6-epimerase

substrate: ADP-heptose


enzyme_display_name: dTDP-4-dehydrorhamnose 3,5-epimerase

organism: Streptococcus suis

enzyme: dTDP-4-dehydrorhamnose 3,5-epimerase


substrate_name: UDP-N-acetyl-alpha-D-glucosamine

column_label: Table 1 reports NmSacA-His6 UDP-GlcNAc kcat 31 +/- 2 s^-1, KM 3.6 +/- 0.5 mM, and kcat/KM 8.6 s^-1 mM^-1; kcat and KM match, but kcat/KM differs from the current export

normalized_value_unit_evidence: kcat normalized_value=31 normalized_unit=s^-1; KM normalized_value=3.6 normalized_unit=mM; kcat_over_km normalized_value=8.6 normalized_unit=s^-1*mM^-1; current_export_kcat_over_km=8.61111111111111 s^-1*mM^-1

raw_value_unit_evidence: kcat raw_value=31 raw_unit=s^-1; KM raw_value=3.6 raw_unit=mM; kcat_over_km raw_value=8.6 raw_unit=s^-1*mM^-1 for UDP-GlcNAc

row_label: NmSacA-His6 UDP-GlcNAc row; kcat, KM, and kcat/KM columns

table_label: PMID 26598987 PMC HTML Table 1

substrate: UDP-N-acetyl-alpha-D-glucosamine


substrate_name: UDP-N-acetyl-alpha-D-mannosamine

column_label: Table 1 reports NmSacA-His6 UDP-ManNAc kcat 52 +/- 5 s^-1, KM 5.2 +/- 1.1 mM, and kcat/KM 10 s^-1 mM^-1; the full current metric tuple matches these explicit paper values

normalized_value_unit_evidence: kcat normalized_value=52 normalized_unit=s^-1; KM normalized_value=5.2 normalized_unit=mM; kcat_over_km normalized_value=10 normalized_unit=s^-1*mM^-1; current export matches all three values

raw_value_unit_evidence: kcat raw_value=52 raw_unit=s^-1; KM raw_value=5.2 raw_unit=mM; kcat_over_km raw_value=10 raw_unit=s^-1*mM^-1 for UDP-ManNAc

row_label: NmSacA-His6 UDP-ManNAc row; kcat, KM, and kcat/KM columns

substrate: UDP-N-acetyl-alpha-D-mannosamine


substrate_name: GDP-D-mannose

substrate: GDP-D-mannose


organism: Sus scrofa


organism: Aneurinibacillus thermoaerophilus

column_label: TABLE 4. Apparent kinetic constants for dTDP-l-rhamnose biosynthetic enzymes; block 'DSM 10155 RmlC'; substrate row 'dTDP-6-deoxy-d-xylo-4- hexulose'; column 'Km (mM)' = 0.062 +/- 0.0063; column 'kcat (s-1)' = 2.0 +/- 0.67; the table has no kcat/Km column.

normalized_value_unit_evidence: Km: paper cell 0.062 mM; the column is already mM, so normalized Km = 0.062 mM. kcat: paper cell 2.0 s-1; the column is already s^(-1), so normalized kcat = 2.0 s^(-1). kcat/Km: not printed anywhere in the paper; the full-precision quotient of the row's own cells is 2.0 / 0.062 = 32.25806451612903 s^(-1)*mM^(-1).

raw_value_unit_evidence: TABLE 4. Apparent kinetic constants for dTDP-l-rhamnose biosynthetic enzymes, block 'DSM 10155 RmlC', substrate row 'dTDP-6-deoxy-d-xylo-4- hexulose': Km = 0.062 +/- 0.0063 mM; kcat = 2.0 +/- 0.67 s-1; no kcat/Km cell is printed.

row_label: Table 4, block 'DSM 10155 RmlC', substrate row 'dTDP-6-deoxy-d-xylo-4- hexulose'


organism: Arabidopsis thaliana

assay_conditions_summary: cofactor NADPH


substrate_name: NADPH

substrate: NADPH


organism: Ruminococcus albus

substrate_name: lactose

proof_lines: F114A mutant, 100 mM sodium phosphate, pH 7.5, 20 mM lactose, 30°C

proof_lines: F114A mutant, 100 mM sodium phosphate, pH 7.5, 20 mM cellobiose, 30°C

substrate: lactose


proof_lines: W303F mutant, 100 mM sodium phosphate, pH 7.5, 20 mM lactose, 30°C

proof_lines: W303F mutant, 100 mM sodium phosphate, pH 7.5, 20 mM cellobiose, 30°C


substrate_name: cellobiose

proof_lines: M378T mutant, 100 mM sodium phosphate, pH 7.5, 20 mM cellobiose, 30°C

substrate: cellobiose


proof_lines: M270T mutant, 100 mM sodium phosphate, pH 7.5, 20 mM cellobiose, 30°C


proof_lines: R377A mutant, 100 mM sodium phosphate, pH 7.5, 20 mM lactose, 30°C

proof_lines: R377A mutant, 100 mM sodium phosphate, pH 7.5, 20 mM cellobiose, 30°C


proof_lines: M106T mutant, 100 mM sodium phosphate, pH 7.5, 20 mM cellobiose, 30°C


proof_lines: M62T mutant, 100 mM sodium phosphate, pH 7.5, 20 mM cellobiose, 30°C


proof_lines: M212T mutant, 100 mM sodium phosphate, pH 7.5, 20 mM cellobiose, 30°C


proof_lines: M95T mutant, 100 mM sodium phosphate, pH 7.5, 20 mM cellobiose, 30°C


proof_lines: M179T mutant, 100 mM sodium phosphate, pH 7.5, 20 mM cellobiose, 30°C


proof_lines: M380T mutant, 100 mM sodium phosphate, pH 7.5, 20 mM cellobiose, 30°C


proof_lines: M96T mutant, 100 mM sodium phosphate, pH 7.5, 20 mM cellobiose, 30°C


proof_lines: wild type, 100 mM sodium phosphate, pH 7.5, 20 mM lactose, 30°C

proof_lines: wild type enzyme, 100 mM sodium phosphate, pH 7.5, 20 mM cellobiose, 30°C


proof_lines: W55F mutant, 100 mM sodium phosphate, pH 7.5, 20 mM lactose, 30°C

proof_lines: W55F mutant, 100 mM sodium phosphate, pH 7.5, 20 mM cellobiose, 30°C


organism: Methanococcus maripaludis (strain DSM 14266 / JCM 13030 / NBRC 101832 / S2 / LL)

substrate_name: UDP-GlcNAc

substrate: UDP-GlcNAc


organism: Salmonella typhimurium


organism: Dictyoglomus turgidum

substrate_name: lactulose

proof_lines:
pH 7.5, 50°C, recombinant enzyme
pH 7.5, 50°C

substrate: lactulose


substrate_name: epilactose

substrate: epilactose


enzyme_display_name: UDP-glucose 4-epimerase

substrate_name: UDP-alpha-D-glucose

assay_conditions_summary: pH 7; 310.15 K; buffer McIlvaine

proof_lines:
pH 7.0, 37°C
24°C, pH 7.0
in McIlvaine buffer (pH 7.5), at 35°C

enzyme: UDP-glucose 4-epimerase

substrate: UDP-alpha-D-glucose


substrate_name: UDP-glucose

substrate: UDP-glucose


substrate_name: UDP-glucuronate

proof_lines: recombinant AtUGlcAE2, lacking the amino acid position 1-68, in 0.1 M sodium phosphate, pH 7.6

proof_lines: recombinant AtUGlcAE3, lacking the amino acid position 1-53, in 0.1 M sodium phosphate, pH 7.6

substrate: UDP-glucuronate


substrate_name: Unknown

proof_lines: recombinant AtUGlcAE3, position 1-53 is lacking

proof_lines: recombinant AtUGlcAE2, position 1-68 is lacking

proof_lines: recombinant AtUGlcAE1, position 1-64 is lacking

substrate: Unknown


organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

proof_lines: kcat is 39 sec(-1) with dTDP-6-deoxy-D-xylo-4-hexulose as substrate.


proof_lines: recombinant enzyme with N-terminal His-tag

proof_lines: recombinant enzyme with N-terminal glutathione-S-transferase tag


organism: Oryza sativa


substrate_name: D-mannose

proof_lines:
pH 7.5, 70°C, wild-type enzyme
pH 6.3, 70°C, wild-type enzyme

substrate: D-mannose


substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


source_license: CC BY 4.0; CC BY-NC-ND 4.0

proof_lines: kcat is 31 sec(-1) with UDP-GlcNAc as substrate. kcat is 52 sec(-1) with UDP-ManNAc as substrate. kcat is 4.2 sec(-1) with UDP-ManNGc as substrate. kcat is 7.8 sec(-1) with UDP-ManNPr as substrate. kcat is 6.7 sec(-1) with rUDP-ManNAc as su

source_databases_merged: brenda skid uniprot


substrate_name: D-galactose

assay_conditions_summary: pH 7.5; 308.15 K; buffer McIlvaine

substrate: D-galactose


substrate_name: D-tagatose

substrate: D-tagatose


organism: Bacillus anthracis


substrate_name: GDP-alpha-D-mannose

proof_lines: pH and temperature not specified in the publication

substrate: GDP-alpha-D-mannose


proof_lines: recombinant OsUGlcAE3, lacking the amino acid position 1-53, in 0.1 M sodium phosphate, pH 7.6


proof_lines: recombinant OsUGlcAE3, position 1-53 is lacking


organism: Oryza sativa subsp. japonica

substrate_name: GDP-mannose

proof_lines: The catalytic efficiency increases by 2-fold in the presence of NAD(+).

substrate: GDP-mannose


organism: Campylobacter jejuni


enzyme_display_name: allantoin racemase

organism: Pseudomonas fluorescens

substrate_name: (R)-allantoin

proof_lines: mutant E78D, pH 7.6, temperature not specified in the publication

enzyme: allantoin racemase

substrate: (R)-allantoin


proof_lines: wild-type, pH 7.6, temperature not specified in the publication


organism: Salmonella enterica


enzyme_display_name: D-cysteine desulfhydrase

organism: Solanum lycopersicum

substrate_name: D-cysteine

smiles: C(C(C(=O)O)N)S

enzyme: D-cysteine desulfhydrase

substrate: D-cysteine


substrate_name: UDPglucuronate

substrate: UDPglucuronate


enzyme_display_name: 16-hydroxysteroid epimerase

organism: Homo sapiens

enzyme: 16-hydroxysteroid epimerase


organism: Salmonella enterica subsp. enterica serovar Typhi


enzyme_display_name: methylmalonyl-CoA epimerase

organism: Rattus norvegicus

substrate_name: DL-methylmalonyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: methylmalonyl-CoA epimerase

substrate: DL-methylmalonyl-CoA


enzyme_display_name: NAD(P)H-hydrate epimerase

organism: Escherichia coli (strain K12)

substrate_name: (S)-NADHX

enzyme: NAD(P)H-hydrate epimerase

substrate: (S)-NADHX


organism: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)

substrate_name: (S)-methylmalonyl-CoA

proof_lines: kcat is 240 sec(-1) for (S)-methylmalonyl-CoA epimerization.

substrate: (S)-methylmalonyl-CoA


organism: Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.)


enzyme_display_name: alpha-methylacyl-CoA racemase

enzyme: alpha-methylacyl-CoA racemase


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

proof_lines: kcat is 3.7 sec(-1) with (25R)-3-oxo-cholest-4-en-26-oyl-CoA as substrate (PubMed:26348625). kcat is 450 sec(-1) with (2S)-ibuprofenoyl-CoA as substrate (PubMed:19854148). kcat is 291 sec(-1) with (2R)-ibuprofenoyl-CoA as substrate (PubMed:


enzyme_display_name: hydantoin racemase

organism: Pseudomonas sp. (strain NS671)

enzyme: hydantoin racemase


organism: Rhizobium meliloti

substrate_name: L-5-ethylhydantoin (L-EH)

proof_lines: kcat is 6.42 sec(-1) with L-5-ethylhydantoin (L-EH) as substrate. kcat is 3.24 sec(-1) with D-5-isobutylhydantoin (D-IBH) as substrate. kcat is 2.29 sec(-1) with D-5-benzylhydantoin (D-BH) as substrate. kcat is 2.12 sec(-1) with L-5-isobuty

substrate: L-5-ethylhydantoin (L-EH)


organism: Mus musculus

substrate_name: (R)-NADH-hydrate

assay_conditions_summary: pH 8; 303.15 K; cofactor ATP

proof_lines: in the presence of 5 mM KCl, at 30°C in 25 mM Tris-HCl, pH 8.0, 2 mM MgCl2, 0.2 mM ATP

substrate: (R)-NADH-hydrate


enzyme_display_name: cellobiose epimerase

organism: Treponema brennaborense

substrate_name: cellobiose

enzyme: cellobiose epimerase

substrate: cellobiose


substrate_name: (R)-pristanoyl-CoA

substrate: (R)-pristanoyl-CoA


organism: Klebsiella pneumoniae subsp. pneumoniae

substrate_name: (S)-allantoin

proof_lines: in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25°C

substrate: (S)-allantoin


substrate_name: pristanoyl-CoA

substrate: pristanoyl-CoA


substrate_name: trihydroxycoprostanoyl-CoA

substrate: trihydroxycoprostanoyl-CoA


substrate_name: 7,8-dihydroneopterin triphosphate

substrate: 7,8-dihydroneopterin triphosphate


organism: Agrobacterium tumefaciens

substrate_name: L-benzylhydantoin

substrate: L-benzylhydantoin


organism: Cereibacter sphaeroides


substrate_name: (S)-NADPHX

column_label: Supplemental Table S3, dehydratase substrate (S)-NADPHX, YjeF row, Km column cell 0.86 +/- 0.32 uM.

normalized_value_unit_evidence: 0.86 uM = 0.00086 mM; matches package/export Km 0.00086 mM.

raw_value_unit_evidence: Supplemental Table S3 reports Km units as uM; (S)-NADPHX row, YjeF enzyme: Km 0.86 +/- 0.32 uM.

row_label: YjeF / (S)-NADPHX

table_label: Supplemental Table S3

substrate: (S)-NADPHX


substrate_name: ADP

assay_conditions_summary: pH 7.1; cofactor ADP

column_label: Supplemental Table S3, ADP row, YjeF enzyme, Km column cell 3.6 +/- 1.1 uM.

raw_value_unit_evidence: Supplemental Table S3 reports Km units as uM; ADP row, YjeF enzyme: Km 3.6 +/- 1.1 uM.

row_label: YjeF / ADP

substrate: ADP


organism: Escherichia coli

substrate_name: 7,8-dihydroneopterin 3'-triphosphate

substrate: 7,8-dihydroneopterin 3'-triphosphate


organism: Salmonella typhi

substrate_name: UDP-GlcNAcA

proof_lines: kcat is 56.8 min(-1) with UDP-GlcNAcA as substrate. kcat is 39.1 min(-1) with UDP-GalNAcA as substrate. kcat is 14.2 min(-1) with UDP-GlcNAc as substrate. kcat is 52.6 min(-1) with UDP-GalNAc as substrate.

substrate: UDP-GlcNAcA


substrate_name: UDP-GalNAcA

substrate: UDP-GalNAcA


substrate_name: UDP-GlcNAc

substrate: UDP-GlcNAc


enzyme_display_name: maleate isomerase

organism: Pseudomonas sp.

substrate_name: Maleate

enzyme: maleate isomerase

substrate: Maleate


organism: Solanum lycopersicum

substrate_name: S-adenosyl-L-ethionine

substrate: S-adenosyl-L-ethionine


substrate_name: Unknown

substrate: Unknown


organism: Sphingobium chungbukense

substrate_name: alpha-D-mannose 1-phosphate

proof_lines: SP-1, in 50 mM Tris-HCl (pH 7.5), at 37°C

substrate: alpha-D-mannose 1-phosphate


enzyme_display_name: phosphomannomutase

substrate_name: alpha-D-glucose 1-phosphate

enzyme: phosphomannomutase

substrate: alpha-D-glucose 1-phosphate


organism: Homo sapiens

substrate_name: (S)-naproxenoyl-CoA

assay_conditions_summary: pH 7.4; 310.15 K; cofactor CoA

proof_lines: anti-enantiomer, pH not specified in the publication, temperature not specified in the publication

proof_lines: pH not specified in the publication, 37°C

substrate: (S)-naproxenoyl-CoA


assay_conditions_summary: pH 7.2; cofactor CoA

proof_lines: pH not specified in the publication, temperature not specified in the publication

proof_lines: recombinant enzyme, 50 mM NaH2PO4-NaOH, pH 7.2


enzyme_display_name: alanine racemase

organism: Staphylococcus aureus

substrate_name: D-alanine

smiles: CC(C(=O)O)N

proof_lines: pH and temperature not specified in the publication

proof_lines: pH not specified in the publication, 30°C, recombinant enzyme

enzyme: alanine racemase

substrate: D-alanine


enzyme_display_name: L-3-cyanoalanine synthase

organism: Spinacia oleracea

substrate_name: KCN

proof_lines:
vs. O-acetyl-L-serine
vs. chloroalanine

enzyme: L-3-cyanoalanine synthase

substrate: KCN


organism: Streptomyces lavendulae

substrate_name: D-serine

smiles: C(C(C(=O)O)N)O

substrate: D-serine


substrate_name: L-alanine

substrate: L-alanine


enzyme_display_name: maleylpyruvate isomerase

organism: Corynebacterium glutamicum

enzyme: maleylpyruvate isomerase


enzyme_display_name: linoleate isomerase

organism: Lactobacillus delbrueckii subsp. bulgaricus

substrate_name: 9-cis,12-cis-linoleic acid

smiles: CCCCCC=CCC=CCCCCCCCC(=O)O

enzyme: linoleate isomerase

substrate: 9-cis,12-cis-linoleic acid


organism: Butyrivibrio fibrisolvens

smiles: CCCCCC=CCC=CCCCCCCCC(=O)[O-]


organism: Mus musculus

substrate_name: (R)-NADPHX

substrate: (R)-NADPHX


organism: Rhizobium meliloti

substrate_name: D-5-isobutylhydantoin (D-IBH)

substrate: D-5-isobutylhydantoin (D-IBH)


substrate_name: L-5-benzylhydantoin (L-BH)

substrate: L-5-benzylhydantoin (L-BH)


organism: Rattus norvegicus

substrate_name: pristanoyl-CoA

substrate: pristanoyl-CoA


substrate_name: trihydroxycoprostanoyl-CoA

substrate: trihydroxycoprostanoyl-CoA


enzyme_display_name: maleylacetoacetate isomerase

organism: Arabidopsis thaliana

enzyme: maleylacetoacetate isomerase


enzyme_display_name: 7,8-dihydroneopterin epimerase

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: 7,8-dihydroneopterin in the aldolase reaction

proof_lines: kcat is 0.0054 sec(-1) for the aldolase reaction with 7,8-dihydroneopterin as substrate. kcat is 0.006 sec(-1) for the aldolase reaction with 7,8-dihydromonapterin as substrate. kcat is 0.0015 sec(-1) for the epimerization of 7,8-dihydroneo

enzyme: 7,8-dihydroneopterin epimerase


organism: Alicyclobacillus acidoterrestris

substrate_name: Maleate


substrate_name: dichloroacetic acid

smiles: C(C(=O)O)(Cl)Cl

substrate: dichloroacetic acid


substrate_name: glutathione

smiles: C(CC(=O)NC(CS)C(=O)NCC(=O)O)C(C(=O)O)N

column_label: Abstract states the Km for glutathione was 59 microM.

raw_value_unit_evidence: Km raw_value=59 raw_unit=microM

row_label: rat liver glutathione transferase zeta with glutathione

table_label: PubMed abstract PMID 9531472

substrate: glutathione


organism: Clostridium sporogenes


smiles: CCC=CCC=CCC=CCCCCCCCC(=O)O


smiles: CCCCCC=CCC=CCC=CCCCCC(=O)O


organism: Pseudomonas aeruginosa

substrate_name: UDP-N-acetylgalactosamine

proof_lines: kcat is 271 min(-1) with UDP-N-acetylgalactosamine as substrate. kcat is 120 min(-1) with UDP-N-acetylglucosamine as substrate. kcat is 0.188 min(-1) with UDP-galactose as substrate. kcat is 0.124 min(-1) with UDP-glucose as substrate.

substrate: UDP-N-acetylgalactosamine


substrate_name: UDP-galactose

substrate: UDP-galactose


organism: Escherichia coli (strain K12)

substrate_name: 7,8-dihydroneopterin in aldolase reaction


organism: Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

substrate: 7,8-dihydroneopterin in aldolase reaction


organism: Homo sapiens


proof_lines:
pH 7.6, 25°C, enzyme hGSTZ1d-1d
pH 7.6, 25°C, enzyme hGSTZ1c-1c
pH 7.6, 25°C, enzyme hGSTZ1b-1b


organism: Nocardia farcinica

proof_lines: mutant enzyme C194S, at pH 7.5, temperature not specified in the publication

substrate: Maleate


proof_lines: mutant enzyme C76S, at pH 7.5, temperature not specified in the publication


proof_lines: mutant enzyme Y133F, at pH 7.5, temperature not specified in the publication


proof_lines: wild type enzyme, at pH 7.5, temperature not specified in the publication


substrate_name: Unknown

proof_lines: pH 7.5, temperature not specified in the publication

substrate: Unknown


organism: Spinacia oleracea

smiles: C(C(C(=O)O)N)Cl


substrate_name: O-acetyl-L-serine

smiles: CC(=O)OCC(C(=O)O)N

proof_lines:
26°C, pH 9.8
vs. chloroalanine

substrate: O-acetyl-L-serine


organism: Escherichia coli

substrate_name: 7,8-dihydroneopterin triphosphate

substrate: 7,8-dihydroneopterin triphosphate


organism: Synechococcus sp.


enzyme_display_name: chorismate mutase

organism: Thermus thermophilus

substrate_name: chorismate

enzyme: chorismate mutase

substrate: chorismate


enzyme_display_name: methionine gamma-lyase

organism: Trichomonas vaginalis

substrate_name: DL-homocysteine

smiles: C(CS)C(C(=O)O)N

enzyme: methionine gamma-lyase

substrate: DL-homocysteine


substrate_name: L-cysteine

substrate: L-cysteine


organism: Serratia marcescens


substrate_name: L-ethionine

substrate: L-ethionine


substrate_name: L-methionine

smiles: CSCCC(C(=O)O)N

substrate: L-methionine


enzyme_display_name: phosphoacetylglucosamine mutase

organism: Trypanosoma brucei

substrate_name: N-acetyl-D-glucosamine 6-phosphate

enzyme: phosphoacetylglucosamine mutase

substrate: N-acetyl-D-glucosamine 6-phosphate


substrate_name: alpha-D-mannose 1-phosphate

substrate: alpha-D-mannose 1-phosphate


organism: Alcaligenes faecalis


substrate_name: maleate

substrate: maleate


organism: Trypanosoma cruzi


substrate_name: D-mannose 6-phosphate

substrate: D-mannose 6-phosphate


enzyme_display_name: protoporphyrin ferrochelatase

organism: Xenopus laevis

substrate_name: protoporphyrin

enzyme: protoporphyrin ferrochelatase

substrate: protoporphyrin


organism: Ralstonia sp.

substrate_name: maleylpyruvate

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature R109A to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 8; buffer 50 mM phosphate

proof_lines: mutant enzyme R109A, in 50 mM phosphate buffer, pH 8.0, at 22°C

substrate: maleylpyruvate


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature R8A/R176A to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme R8A/R176A, in 50 mM phosphate buffer, pH 8.0, at 22°C


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature H104A to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme H104A, in 50 mM phosphate buffer, pH 8.0, at 22°C


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature Q49A to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme Q49A, in 50 mM phosphate buffer, pH 8.0, at 22°C


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature R8A to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme R8A, in 50 mM phosphate buffer, pH 8.0, at 22°C


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature N108A with inferred residue-numbering offset -1 to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme N108A, in 50 mM phosphate buffer, pH 8.0, at 22°C


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature R176A to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme R176A, in 50 mM phosphate buffer, pH 8.0, at 22°C


proof_lines: wild type enzyme, in 50 mM phosphate buffer, pH 8.0, at 22°C


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature T11A to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme T11A, in 50 mM phosphate buffer, pH 8.0, at 22°C


enzyme_display_name: S-ribosylhomocysteine lyase

organism: Vibrio cholerae

proof_lines: pH and temperature not specified in the publication

enzyme: S-ribosylhomocysteine lyase


enzyme_display_name: lactoylglutathione lyase

organism: Sus scrofa

substrate_name: Phenylglyoxal

enzyme: lactoylglutathione lyase

substrate: Phenylglyoxal


substrate_name: methylglyoxal

substrate: methylglyoxal


organism: Yersinia pestis

smiles: CC(=O)C=O

proof_lines:
presence of Co2+, pH 7.0
presence of Ni2+, pH 7.0


substrate_name: (R)-S-lactoylglutathione

proof_lines:
pH 7.0, 0.5 mM CoCl2
pH 7.0, 0.5 mM NiCl2

substrate: (R)-S-lactoylglutathione


substrate_name: Maleate

source_db: skid

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines:
Raw SKiD Env: Temp=-----, pH=7.5
kcat is 2.8 sec(-1).

source_databases_merged: skid uniprot

substrate: Maleate

source: skid


enzyme_display_name: proline racemase

substrate_name: L-proline

enzyme: proline racemase

substrate: L-proline


substrate_name: Unknown

proof_lines: recombinant His-tagged enzyme, pH 6.0, 37°C

substrate: Unknown


enzyme_display_name: alpha-methylacyl-CoA racemase

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

proof_lines: kcat is 3.7 sec(-1) with (25R)-3-oxo-cholest-4-en-26-oyl-CoA as substrate (PubMed:26348625). kcat is 450 sec(-1) with (2S)-ibuprofenoyl-CoA as substrate (PubMed:19854148). kcat is 291 sec(-1) with (2R)-ibuprofenoyl-CoA as substrate (PubMed:

enzyme: alpha-methylacyl-CoA racemase


enzyme_display_name: D-ornithine 4,5-aminomutase

organism: Acetoanaerobium sticklandii

substrate_name: D-ornithine

smiles: C(CC(C(=O)O)N)CN

enzyme: D-ornithine 4,5-aminomutase

substrate: D-ornithine


proof_lines: pH 8.5, 25°C, recombinant wild-type enzyme


enzyme_display_name: lysine 5,6-aminomutase

substrate_name: D-Lysine

smiles: C(CCN)CC(C(=O)O)N

proof_lines: with pyridoxal 5'-phosphate N-oxide as cofactor, pH 8.5, 37°C

proof_lines: with pyridoxal 5'-phosphate as cofactor, pH 8.5, 37°C

enzyme: lysine 5,6-aminomutase

substrate: D-Lysine


enzyme_display_name: triose-phosphate isomerase

organism: Oryctolagus cuniculus

substrate_name: D-glyceraldehyde 3-phosphate

smiles: C(C(C=O)O)OP(=O)(O)O

enzyme: triose-phosphate isomerase

substrate: D-glyceraldehyde 3-phosphate


enzyme_display_name: UniProt P00940

organism: Gallus gallus

substrate_name: dihydroxyacetone 3-phosphate

source_license: CC BY-NC-ND 4.0

smiles: C(C(=O)COP(=O)(O)O)O

enzyme: UniProt P00940

substrate: dihydroxyacetone 3-phosphate


proof_lines:
Raw SKiD Env: Temp=30°C, pH=7.6
Raw SKiD Env: Temp=25°C, pH=7.5


enzyme_display_name: UniProt P36186

organism: Giardia intestinalis

proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=25°C, pH=7.4

enzyme: UniProt P36186


enzyme_display_name: UniProt P04789

enzyme: UniProt P04789


enzyme_display_name: UniProt A2FT29

enzyme: UniProt A2FT29


enzyme_display_name: UDP-glucuronate 4-epimerase

organism: Zea mays

substrate_name: UDP-glucuronate

proof_lines: recombinant ZmUGlcAE, lacking the putative transmembrane domain at amino acid position 1-47, in 0.1 M sodium phosphate, pH 7.6

enzyme: UDP-glucuronate 4-epimerase

substrate: UDP-glucuronate


proof_lines: recombinant ZmUGlcAE, position 1-47 is lacking


smiles: C(CC(CC(=O)O)N)CN


organism: Vibrio vulnificus

proof_lines: in complex with protoporphyrinogen dehydrogenase PgdH1, pH 8.0, 30°C


enzyme_display_name: alanine racemase

organism: Acinetobacter baumannii

substrate_name: D-alanine

smiles: CC(C(=O)O)N

proof_lines: pH not specified in the publication, 30°C

enzyme: alanine racemase

substrate: D-alanine


enzyme_display_name: chorismate mutase

organism: Acinetobacter calcoaceticus

substrate_name: chorismate

enzyme: chorismate mutase

substrate: chorismate


organism: Lathyrus oleraceus


substrate_name: glycerone phosphate

substrate: glycerone phosphate


organism: Pteris vittata


organism: Homo sapiens

proof_lines:
isoenzyme B
isoenzyme A
enzyme form III


substrate_name: L-Pro

substrate: L-Pro


organism: Saccharomyces cerevisiae

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_consensus_sequence and applied mutation signature Y208F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_consensus_sequence and applied mutation signature Y208T/S211G to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_consensus_sequence and applied mutation signature Y208A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_consensus_sequence and applied mutation signature Y208S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_consensus_sequence and applied mutation signature Y208T to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_consensus_sequence and applied mutation signature S211G to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_consensus_sequence and applied mutation signature S211A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_consensus_sequence and applied mutation signature K17L/Y46F/D48F/Q82F/D85L to reconstruct the assayed variant sequence.


sequence_variant_note: Mutation signature K17L/Y46F/D48Y/Q82A/D85A could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature K17P/Y46A/D48L/Q82T/D85A could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature K17A/Y46A/D48F/Q82A/D85S could not be applied safely because the resolved sequence does not match the expected reference residue(s).


substrate_name: dihydroxyacetone phosphate

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

assay_conditions_summary: pH 7.5; 298.15 K; buffer 30 mM triethanolamine

proof_lines: mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C

substrate: dihydroxyacetone phosphate


proof_lines: wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C


organism: Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM)

substrate_name: D-glyceraldehyde-3-phosphate (at pH 7.4)

proof_lines: kcat is 4016 sec(-1) with D-glyceraldehyde-3-phosphate as substrate (at pH 7.4).


organism: Chlamydomonas reinhardtii

proof_lines: in 100 mM triethanolamine (pH 7.4), at 25°C


organism: Moritella marina

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature A238S to reconstruct the assayed variant sequence.

proof_lines:
pH 7.6, mutant enzyme A238S
mutant A238S


sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

proof_lines: kcat is 420000 min(-1) for isomerase activity with D-glyceraldehyde 3-phosphate as substrate (at pH 7.6 and 10 degrees Celsius).


organism: Giardia duodenalis

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature W162F with inferred residue-numbering offset -9 to reconstruct the assayed variant sequence.


organism: Helicobacter pylori

proof_lines: kcat is 88000 min(-1) for isomerase activity with D-glyceraldehyde 3-phosphate as substrate (at pH 7.5 and 25 degrees Celsius).


organism: Escherichia coli (strain K12)

substrate_name: D-glyceraldehyde 3-phosphate (at 25 degrees Celsius)

proof_lines: kcat is 54000 min(-1) for isomerase activity with D-glyceraldehyde 3-phosphate as substrate (at 25 degrees Celsius).


sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines:
active form P2 of TIM, at 25°C, pH 7.4
isoenzyme A
active form P1 of TIM, at 25°C, pH 7.4


organism: Trichomonas vaginalis

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature V45A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_consensus_sequence and applied mutation signature K12G to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme K12G, in 30 mM TEA at pH 7.5, at 25°C


organism: Trypanosoma

substrate_name: D-Glyceraldehyde 3-phosphate

substrate: D-Glyceraldehyde 3-phosphate


organism: Escherichia coli

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


organism: Mycobacterium tuberculosis

proof_lines: kcat is 4.3 min(-1) for isomerase activity.


organism: Gallus gallus

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature V167G/W168G/K174G/T175G/A176G to reconstruct the assayed variant sequence.

proof_lines: 30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature K174G/T175G/A176G to reconstruct the assayed variant sequence.

proof_lines: 30°C, pH 7.6, mutant enzyme K174G/T175G/A176G


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature V167G/W168G to reconstruct the assayed variant sequence.


enzyme_display_name: peptidylprolyl isomerase

substrate_name: RNase T1

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature V72P to reconstruct the assayed variant sequence.

proof_lines: mutant V72P, pH not specified in the publication, temperature not specified in the publication

enzyme: peptidylprolyl isomerase

substrate: RNase T1


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature I65P to reconstruct the assayed variant sequence.

proof_lines: mutant I65P, pH not specified in the publication, temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature C113S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature K63A with inferred residue-numbering offset +14 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature C113D to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature R68A/R69A to reconstruct the assayed variant sequence.


organism: Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

proof_lines: kcat is 5668 sec(-1) for isomerase activity.


organism: Tenebrio molitor


organism: Thermococcus onnurineus


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)


organism: Oryctolagus cuniculus


organism: Pyrococcus furiosus

sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset -15, so it is treated as a source-provided variant sequence.

assay_conditions_summary: pH 8


organism: Saccharolobus solfataricus


organism: Mycoplasmoides genitalium


sequence_variant_note: Mutation signature S54G/P55N could not be applied safely because the resolved sequence does not match the expected reference residue(s).

proof_lines: disulfide-reduced and S-carboxymethylated form of a variant of Rnase T1 with Ser54Gly and Pro55Asn


organism: Saccharomyces cerevisiae

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature D205G with inferred residue-numbering offset -3 to reconstruct the assayed variant sequence.

proof_lines: 20°C, pH 7.4, mutant D205G of Ypa1, presence of 1 mM MgATP


organism: Clostridium perfringens (strain 13 / Type A)

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

proof_lines: kcat is 6348 sec(-1) for isomerase activity.


organism: Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1)

proof_lines: kcat is 5678 sec(-1) for isomerase activity.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_consensus_sequence and applied mutation signature Y208F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_consensus_sequence and applied mutation signature Y208S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_consensus_sequence and applied mutation signature S211A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_consensus_sequence and applied mutation signature Y208T/S211G to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_consensus_sequence and applied mutation signature Y208A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_consensus_sequence and applied mutation signature Y208T to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature K12G to reconstruct the assayed variant sequence.


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: wild type enzyme, in 30 mM TEA at pH 7.5, at 25°C


proof_lines:
at pH 7.5 and 37°C
Raw SKiD Env: Temp=25°C, pH=7.4
Raw SKiD Env: Temp=25°C, pH=7.5

source_databases_merged: brenda skid


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S211G to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S211A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature V167G/W168G/K174G/T175G/A176G to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature V167G/W168G to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature K174G/T175G/A176G to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature I45Y to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature I45F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature I45G to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature I45A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature I45V to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature I45L to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


proof_lines:
25°C, pH 7.4, wild-type enzyme
wild-type, pH 7.5, 25°C


sequence_variant_note: Mutation signature C126A could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature 173del to reconstruct the assayed variant sequence.

mutation_type: deletion


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature V167P/W168E to reconstruct the assayed variant sequence.


organism: Arabidopsis thaliana

sequence_variant_note: Resolved canonical sequence from official_uniprot_fasta_exact_accession and applied mutation signature C127S to reconstruct the assayed variant sequence.

table_label: Table 1


sequence_variant_note: Resolved canonical sequence from official_uniprot_fasta_exact_accession and applied mutation signature C127S/C218S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from official_uniprot_fasta_exact_accession and applied mutation signature C218S to reconstruct the assayed variant sequence.


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

row_label: WT


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature E165D to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature E165D/E97D with inferred residue-numbering offset -20 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature E165D/G10S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature E165D/G233R to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature E165D/S96P to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature E165D/S96T to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature E165D/V167D with inferred residue-numbering offset -25 to reconstruct the assayed variant sequence.


smiles: O=C(CO)COP(=O)(O)O


proof_lines: The kcat is 900 sec(-1) for the isomerization of dihydroxyacetone phosphate (PubMed:2021650). The kcat is 0.011 sec(-1) for the production of methylglyoxal via the elimination reaction (PubMed:2021650). Produces methylglyoxal with a calcula


organism: Plasmodium falciparum

proof_lines: kcat is 4300 sec(-1) with D-glyceraldehyde-3-phosphate as substrate (at 25 degrees Celsius) (PubMed:26762569). kcat is 4233 sec(-1) with D-glyceraldehyde-3-phosphate as substrate (at pH 7.6) (PubMed:19622869).


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature C126A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature C126M to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature C126S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature C126T to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature C126V to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature S211A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature S211G to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature Y208A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature Y208F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature Y208S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature Y208T to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature Y208T/S211G to reconstruct the assayed variant sequence.


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_consensus_sequence and applied mutation signature D225Q to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature T75S with inferred residue-numbering offset -14 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature T75V with inferred residue-numbering offset -14 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature T75C with inferred residue-numbering offset -14 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature T75N with inferred residue-numbering offset -14 to reconstruct the assayed variant sequence.


organism: Trypanosoma cruzi

sequence_variant_note: Mutation signature S20Q/L47V/I49L/P50A/Y87F/I89V could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature E165A with inferred residue-numbering offset +14 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature E97Q to reconstruct the assayed variant sequence.


organism: Clostridium perfringens


column_label: Table 2 reports KM 8.4 x 10^-4 M, kcat 4300 s^-1, and catalytic efficiency 5.1 x 10^6 s^-1 M^-1 for Tenebrio molitor TIM.

normalized_value_unit_evidence: Km normalized_value=0.84 normalized_unit=mM; kcat normalized_value=4300 normalized_unit=s^-1; kcat/Km normalized_value=5100 normalized_unit=s^-1 mM^-1

raw_value_unit_evidence: Km raw_value=8.4 x 10^-4 raw_unit=M; kcat raw_value=4300 raw_unit=s^-1; kcat/Km raw_value=5.1 x 10^6 raw_unit=s^-1 M^-1

row_label: Tenebrio molitor / glyceraldehyde 3-phosphate

table_label: Wiley Table 2, kinetic parameters for Tenebrio molitor TIM


enzyme_display_name: beta-carotene isomerase

organism: Oryza sativa Japonica Group

column_label: Chrome-visible Wiley full text states: The KM for 9-cis-beta-carotene in the reverse reaction was determined to be 0.26 uM, with a kcat value of 34 s^-1.

raw_value_unit_evidence: KM raw_value=0.26 uM raw_unit=uM

row_label: OsD27 reverse reaction / 9-cis-beta-carotene

table_label: Wiley/FEBS Journal full text prose / Biochemical characterization of OsD27

enzyme: beta-carotene isomerase


organism: Homo sapiens

substrate_name: Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide

substrate: Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide


column_label: Results text reports kcat 960 s^-1 and Km 230 microM for the tetrapeptide substrate; 230 microM converts to 0.23 mM.

raw_value_unit_evidence: Km raw_value=230 microM raw_unit=microM

row_label: Human PPIL1 recombinant enzyme / N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide

table_label: JBC full text PMID 16595688 Results / PPIase activity assay


organism: Nostoc punctiforme


organism: Rattus norvegicus


organism: Deinococcus radiodurans


organism: Trichomonas vaginalis

sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature I45A to reconstruct the assayed variant sequence.


sequence_variant_note: Mutation signature I45F could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset -11, so it is treated as a source-provided variant sequence.


sequence_variant_note: Mutation signature I45L could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature I45V could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature I45Y could not be applied safely because the resolved sequence does not match the expected reference residue(s).