organism: Galerina marginata

substrate_name: IWGIGCNPWTAEHVDQTLASGNDIC

proof_lines:
at pH 8.0 and 37°C
at pH 8.0 and 20°C

substrate: IWGIGCNPWTAEHVDQTLASGNDIC


enzyme_display_name: prolyl oligopeptidase

organism: Homo sapiens

source_db: brenda

ki_display: Not reported

kcat_over_km_display: Not reported

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: mutant enzyme T202C/T590C, at pH 8.0 and 25°C

enzyme: prolyl oligopeptidase

source: brenda


mutation_signature: Unknown

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


kcat_display: Not reported

proof_lines:
pH 8.0, 37°C
human brain
intracellular enzyme


organism: Lyophyllum cinerascens

substrate_name: Benzyloxycarbonyl-Gly-Pro-Ala

substrate: Benzyloxycarbonyl-Gly-Pro-Ala


organism: Mus musculus

proof_lines: pH 7.4, 37°C, enzyme from thioglycollate-elicited macrophage

proof_lines: pH and temperature not specified in the publication


enzyme_display_name: flavin-containing monooxygenase

substrate_name: Ethionamide

protein_accession_database: UniProt

enzyme: flavin-containing monooxygenase

substrate: Ethionamide


substrate_name: thiacetazone

assay_conditions_summary: pH 9.5; 310.15 K; buffer 0.1 M potassium phosphate

substrate: thiacetazone


substrate_name: methyl p-tolyl sulfide

mutation_type: substitution

assay_conditions_summary: pH 9; 310.15 K; cofactor NADPH

substrate: methyl p-tolyl sulfide


substrate_name: NADPH

substrate: NADPH


substrate_name: calcitonin gene-related peptide

proof_lines: mutant D35A/K196A, diserine mutant of calcitonin gene-related peptide

proof_lines: mutant D35A/K196A, shorter peptide of calcitonin gene-related peptide

proof_lines: mutant D35A/K196A, full length calcitonin gene-related peptide

substrate: calcitonin gene-related peptide


proof_lines: wild type enzyme, full length calcitonin gene-related peptide

proof_lines: wild type enzyme, diserine mutant of calcitonin gene-related peptide

proof_lines: wild type enzyme, shorter peptide of calcitonin gene-related peptide


organism: Myxococcus xanthus


organism: Oryctolagus cuniculus

substrate_name: Unknown

km_display: Not reported

ki_unit: mM

substrate: Unknown


organism: Ovis aries

substrate_name: benzyloxycarbonyl-Gly-Pro-D-Leu

substrate: benzyloxycarbonyl-Gly-Pro-D-Leu


organism: Pleurotus eryngii

proof_lines: mutant enzyme M203Q/M570Q, at pH 8.0 and 25°C


proof_lines: oxidized wild type enzyme, at pH 8.0 and 25°C


proof_lines: mutant enzyme M142Q/M203Q/M570Q/M620Q, at pH 8.0 and 25°C


assay_conditions_summary: pH 8.4; 310.15 K; buffer 0.1 M potassium phosphate

proof_lines: isozyme FMO5, in 0.1 M potassium phosphate buffer (pH 8.4) at 37°C


substrate_name: clomiphene

proof_lines: recombinant His-tagged full-length mutant E158K, pH 7.4, 37°C

proof_lines: recombinant His-tagged truncated mutant E158K, pH 7.4, 37°C

substrate: clomiphene


proof_lines: recombinant His-tagged truncated mutant V257M, pH 7.4, 37°C

proof_lines: recombinant His-tagged full-length mutant V257M, pH 7.4, 37°C


substrate_name: tamoxifen

proof_lines: recombinant His-tagged truncated mutant E308G, pH 7.4, 37°C

proof_lines: recombinant His-tagged full-length mutant E308G, pH 7.4, 37°C

substrate: tamoxifen


substrate_name: Benzydamine

proof_lines: recombinant His-tagged full-length wild-type enzyme, pH 7.4, 37°C

proof_lines: recombinant wild-type enzyme, pH 7.4, 37°C

proof_lines: recombinant His-tagged truncated wild-type enzyme, pH 7.4, 37°C

substrate: Benzydamine


organism: Methylophaga aminisulfidivorans

substrate_name: indole

assay_conditions_summary: pH 8

proof_lines: wild-type, pH 8.0, temperature not specified in the publication

substrate: indole


proof_lines: mutant Y207S, pH 8.0, temperature not specified in the publication


organism: Rattus norvegicus

substrate_name: seleno-L-methionine

proof_lines:
pH 7.6, 37°C, purified FMO3 from liver
pH 7.6, 37°C, purified FMO3 from kidney

substrate: seleno-L-methionine


proof_lines: pH 8.5, 37°C, recombinant mutant isozyme FMO3 E158K

proof_lines: pH 8.5, 37°C, recombinant isozyme FMO1 and recombinant mutant isozyme FMO3 E158K


substrate_name: ethylenethiourea

proof_lines: pH 8.5, 37°C, recombinant wild-type isozyme FMO3

proof_lines: pH 8.4, 37°C, recombinant wild-type His6-tagged MBT-fusion-FMO1

substrate: ethylenethiourea


proof_lines: pH 8.5, 37°C, detergent is a minimal detergent, commercial recombinant FMO3

proof_lines: pH 8.5, 37°C, detergent is CHAPS, recombinant MBP-FMO3


proof_lines:
pH 8.5, FMO1
37°C, isozyme FMO3
pH 8.5, FMO2


organism: Callithrix jacchus

proof_lines: isoform FMO3, pH and temperature not specified in the publication


organism: Canis lupus familiaris

substrate_name: imipramine

substrate: imipramine


substrate_name: fenthion

substrate: fenthion


substrate_name: mercaptoimidazole

proof_lines: pH 8.4, 37°C, recombinant mutant H360P His6-tagged MBT-fusion-FMO1

substrate: mercaptoimidazole


proof_lines: pH 8.4, 37°C, recombinant mutant L360P His6-tagged MBT-fusion-FMO3


substrate_name: chlorpromazine

proof_lines: pH 8.4, 37°C, recombinant mutant L360A His6-tagged MBT-fusion-FMO3

substrate: chlorpromazine


proof_lines: pH 8.4, 37°C, recombinant mutant L360H His6-tagged MBT-fusion-FMO3


proof_lines: pH 8.4, 37°C, recombinant mutant L360Q His6-tagged MBT-fusion-FMO3


substrate_name: Methimazole

substrate: Methimazole


assay_conditions_summary: pH 8.5; 298.15 K; cofactor NADPH


organism: Methylophaga sp.

substrate_name: trimethylamine

proof_lines: pH 8.0, 25°C, recombinant wild-type enzyme

substrate: trimethylamine


proof_lines: pH 8.0, 25°C, recombinant C78I/Y207W/W319A


organism: Mycobacterium tuberculosis

substrate_name: thiacetazone

proof_lines: recombinant EtaA, pH 9.0, 37°C, thiacetazone carbodiimide formation

proof_lines: recombinant EtaA, pH 9.0, 37°C, thiacetazone sulfinic acid formation

substrate: thiacetazone


substrate_name: L-methionine

proof_lines:
recombinant FMO2, pH 7.4, 37°C
recombinant FMO1, pH 7.4, 37°C

substrate: L-methionine


organism: Rhodococcus jostii

substrate_name: Cyclobutanone

assay_conditions_summary: pH 8; 293.15 K; cofactor NADPH

proof_lines: with bicyclo[3.2.0]hept-2-en-6-one as cosubstrate, at pH 8.0 and 20°C

substrate: Cyclobutanone


organism: Squalus acanthias

substrate_name: N,N-Dimethylaniline

substrate: N,N-Dimethylaniline


organism: Sus scrofa

substrate_name: N-Aminopiperidine

assay_conditions_summary: pH 8; 298.15 K; cofactor NADPH

substrate: N-Aminopiperidine


organism: Pyrococcus furiosus


substrate_name: Unknown

proof_lines:
rat brain
rat cortex

substrate: Unknown


organism: Setaria cervi


proof_lines: pH 7.5, 37°C, recombinant wild-type enzyme and mutant C255T


organism: Bacillus subtilis

substrate_name: lichenan

proof_lines: pH 6.4, 40°C, substrate from Hordeum vulgare

proof_lines: pH 6.4, 40°C, purified recombinant His-tagged enzyme, substrate from Hordeum vulgare

substrate: lichenan


organism: Laceyella putida

substrate_name: laminaritetraose

substrate: laminaritetraose


organism: Acetivibrio thermocellus

substrate_name: beta-Glucan

substrate: beta-Glucan


organism: Aplysia kurodai


organism: Botrytis cinerea

substrate_name: D-glucose

substrate: D-glucose


organism: Cyberlindnera jadinii

substrate_name: Laminarin

substrate: Laminarin


organism: Paenibacillus macerans


organism: Periplaneta americana


organism: Rasamsonia emersonii

substrate_name: Barley beta-glucan

proof_lines: pH 5, 50°C, soluble, from barley, EG VII

substrate: Barley beta-glucan


organism: Rhizoctonia solani

substrate_name: short-chain pachyman

substrate: short-chain pachyman


organism: Rhizomucor miehei


organism: Thermothelomyces heterothallicus


enzyme_display_name: tryptophan synthase

organism: Arabidopsis thaliana

substrate_name: L-serine

proof_lines: recombinant isozyme AtTSBtype2, pH 8.2, 30°C

enzyme: tryptophan synthase

substrate: L-serine


organism: Archaeoglobus fulgidus

substrate_name: indole

substrate: indole


organism: Chlamydia trachomatis

proof_lines: TrpAB complex, pH 8.5, temperature not specified in the publication

proof_lines: isolated TrbB subunit, pH 8.5, temperature not specified in the publication


organism: Escherichia coli

proof_lines: alpha-subunit, mutant T183V/A158G, pH 7.6, 25°C


proof_lines: alpha-subunit, mutant T183V/A180G, pH 7.6, 25°C


proof_lines: alpha-subunit, mutant T183V/A71G, pH 7.6, 25°C


proof_lines: alpha-subunit, mutant T183V/A59G, pH 7.6, 25°C


proof_lines: alpha-subunit, mutant T183V, pH 7.6, 25°C


proof_lines: alpha-subunit, mutant T183V/A185G, pH 7.6, 25°C


proof_lines: alpha-subunit, mutant A71G, pH 7.6, 25°C


proof_lines: alpha-subunit, mutant T183V/A67G, pH 7.6, 25°C


proof_lines: reaction of TrpA/TrpB complex, wild-type, pH 7.5, temperature not specified in the publication


proof_lines: cosubstrate 5-hydroxyindole, wild-type, pH 8.5, 35Â°C


proof_lines: cosubstrate 5-hydroxyindole, mutant A67M, pH 8.5, 35Â°C


proof_lines: cosubstrate 5-hydroxyindole, mutant L80H, pH 8.5, 35Â°C


proof_lines: cosubstrate 5-hydroxyindole, mutant D381G, pH 8.5, 35Â°C


proof_lines: cosubstrate 5-hydroxyindole, mutant N64G/S352P, pH 8.5, 35Â°C


proof_lines: cosubstrate 5-hydroxyindole, mutant P257R, pH 8.5, 35Â°C


proof_lines: cosubstrate 5-hydroxyindole, mutant V231S/K382G, pH 8.5, 35Â°C


proof_lines: cosubstrate 5-hydroxyindole, mutant H313F, pH 8.5, 35Â°C


proof_lines: cosubstrate 5-hydroxyindole, mutant T319S, pH 8.5, 35Â°C


proof_lines: reaction of TrpA/TrpB complex, mutant A198W, pH 7.5, temperature not specified in the publication

proof_lines: reaction of subunit TrpB, mutant A198W, pH 7.5, temperature not specified in the publication

proof_lines: reaction of subunit TrpA, mutant A198W, pH 7.5, temperature not specified in the publication


organism: Psilocybe cubensis


organism: Pyrococcus furiosus

proof_lines: subunit TrpB1, mutant E17G/I68V/F274S/T292S/T321A, pH 8.0, 75°C


proof_lines: subunit TrpB1, mutant T292S, pH 8.0, 75°C


proof_lines:
subunit TrpB1, wild-type, pH 8.0, 75°C
wild-type, pH 8.0, 75°C
wild-type holoenzyme, pH 8.0, 75°C


proof_lines: subunit TrpB1, mutant P12L/E17G/I68V/F274S/T292S/T321A, pH 8.0, 75°C


proof_lines: cosubstrate L-serine, pH 8.0, temperature not specified in the publication

proof_lines: cosubstrate L-threonine, pH 8.0, temperature not specified in the publication


organism: Saccharolobus solfataricus

substrate_name: O-phospho-L-serine

proof_lines:
isoform TrpB2a, pH 7.5, 60°C
isoform TrpB2i, pH 7.5, 60°C

substrate: O-phospho-L-serine


organism: Salmonella enterica subsp. enterica serovar Typhimurium

proof_lines: mutant Q114A, pH not specified in the publication, temperature not specified in the publication


proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


organism: Thermococcus kodakarensis

proof_lines: pH 7.5, 85°C, the reaction is catalyzed by the complex of TrpB1 and TrpA

proof_lines: pH 7.5, 85°C, he reaction is catalyzed by TrpB1


organism: Thermotoga maritima


organism: Zea mays

proof_lines: recombinant isozyme TSB type 2, pH 8.2, 30°C


proof_lines: competitive in the catalysis of indoleglycerol phosphate cleavage


organism: Lathyrus oleraceus

proof_lines:
cosubstrate L-Ser
cosubstrate indole


organism: Mycobacterium tuberculosis


organism: Bacillus subtilis


organism: Bos taurus

proof_lines: pH not specified in the publication, temperature not specified in the publication, recombinant wild-type enzyme and mutant D396A


proof_lines: pH not specified in the publication, temperature not specified in the publication, recombinant mutant Y115A


proof_lines: pH not specified in the publication, temperature not specified in the publication, recombinant mutant G319D


proof_lines: pH not specified in the publication, temperature not specified in the publication, recombinant mutant K311A


substrate_name: (Polyphosphate)n

substrate: (Polyphosphate)n


substrate_name: p-nitrophenyl phosphate

substrate: p-nitrophenyl phosphate


proof_lines:
wild-type-type, pH 7.5, 25°C
wild-type, pH 7.5, 25°C


organism: Helicobacter pylori


organism: Homo sapiens

substrate_name: dCMP

assay_conditions_summary: pH 7; 310.15 K; cofactor ADP

proof_lines:
pH 7.0, 37°C, in presence of 1 mM ADP
pH 7.0, 37°C, without ADP

substrate: dCMP


substrate_name: Inosine

assay_conditions_summary: pH 7.4; 310.15 K; buffer mapping; cofactor ATP

proof_lines: presence of 2 mM ATP, Tris-buffer, pH 7.4

proof_lines: presence of 0.4 mM 2,3-bisphosphoglycerate, Tris-buffer, pH 7.4

proof_lines: presence of 2 mM ATP, phosphate-buffered saline, pH 7.4

substrate: Inosine


proof_lines: 37°C, pH not specified in the publication


proof_lines: pH and temperature not specified in the publication


organism: Legionella pneumophila

proof_lines: biphasic kinetic profile, pH 8.0, temperature not specified in the publication

proof_lines: sigmoidal kinetic profile, pH 8.0, temperature not specified in the publication


organism: Mus musculus


organism: Ophiocordyceps sinensis

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Phaseolus vulgaris

substrate_name: TMP

substrate: TMP


organism: Pseudomonas aeruginosa


organism: Rattus norvegicus


organism: Streptococcus pyogenes

proof_lines: Km above 0.8 mM, mutant enzyme R392A, at pH 7.4 and 37°C


organism: Trichinella spiralis


organism: Xylella fastidiosa

substrate_name: 4-nitrophenyl phosphate

substrate: 4-nitrophenyl phosphate


enzyme_display_name: penicillin amidase

organism: Achromobacter xylosoxidans

substrate_name: penicillin G

enzyme: penicillin amidase

substrate: penicillin G


substrate_name: cefamandole

proof_lines: mutant alphaR141A/alphaF142I/betaF24G, pH 6.5, 25°C

substrate: cefamandole


substrate_name: (+)-methyl mandelate

substrate: (+)-methyl mandelate


organism: Escherichia coli

proof_lines:
alphaF146Y mutant protein, pH 7.5, 25°C
alphaF146A mutant protein, pH 7.5, 25°C
betaF24A mutant protein, pH 7.5, 25°C


substrate_name: D-4-hydroxyphenylglycine amide

proof_lines: pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma

proof_lines: enzyme immobilized on amino functionalized magnetic Ni(0.3)Mg(0.4)Zn(0.3)Fe2O4 nanoparticles, pH and temperature not specified in the publication

substrate: D-4-hydroxyphenylglycine amide


organism: Kluyvera cryocrescens


organism: Lysinibacillus sphaericus

substrate_name: Penicillin V

proof_lines: pH 6.0, 37°C, mutant enzyme T63S/S110C/N198Y

substrate: Penicillin V


organism: Priestia megaterium


organism: Providencia rettgeri

substrate_name: amoxicillin

proof_lines: pH 7.0, 30°C, cross-linked enzyme aggregates

proof_lines: pH 7.0, 30°C, poly-lysine supported cross-linked enzyme aggregates

substrate: amoxicillin


organism: Rhizobium viscosum


organism: Streptomyces lavendulae subsp. lavendulae

substrate_name: penicillin F

substrate: penicillin F


organism: Streptomyces mobaraensis


organism: Thermus thermophilus

proof_lines: pH 5.5, 65°C, recombinant mutant L188F/S189F

proof_lines: pH 5.5, 65°C, recombinant mutants L188F and S189F


substrate_name: penicillin K

proof_lines: pH 5.5, 65°C, recombinant wild-type enzyme

substrate: penicillin K


proof_lines: pH 5.5, 65°C, recombinant mutant L188F/L24F/I57F


proof_lines: pH 5.5, 65°C, recombinant mutant L24F/I57F


organism: uncultured bacterium

substrate_name: ampicillin

substrate: ampicillin


organism: Bos taurus

proof_lines:
isoenzyme 5-NT-3
isoenzyme 5-NT-1
isoenzyme 5-NT-2 and 5-NT-4


organism: Unknown


enzyme_display_name: aryl sulfotransferase

substrate_name: Unknown

proof_lines: pH 7.4, 25°C, recombinant SULT1A1, method B

proof_lines: pH 7.4, 25°C, recombinant SULT1A1, method A

enzyme: aryl sulfotransferase

substrate: Unknown


substrate_name: 3'-phosphoadenylyl sulfate

proof_lines: mutant W53L, pH 7.4, temperature not specified in the publication

substrate: 3'-phosphoadenylyl sulfate


proof_lines: mutant W53Y, pH 7.4, temperature not specified in the publication


proof_lines: wild-type, pH 7.4, temperature not specified in the publication


proof_lines: mutant W53A, pH 7.4, temperature not specified in the publication


organism: Canis lupus familiaris

substrate_name: dopamine

substrate: dopamine


organism: Homo sapiens

proof_lines: wild-type SULT1A3: native enzyme, and recombinant from COS-7 cells, Escherichia coli, Saccharomyces cerevisiae, pH 7.4, 37°C

proof_lines: wild-type SULT1A3: purified recombinant enzyme from Escherichia coli, pH 7.4, 37°C


assay_conditions_summary: pH 7; 310.15 K; buffer 50 mM Tris/HCl

proof_lines:
SULT1A1 allozyme 2, pH 7.0, 37°C
pH 6.5, 37°C, allozyme SULT1A1*2
pH 6.5, 37°C, allozyme SULT1A1*3


substrate_name: serotonin

proof_lines: dopamine 3-O-sulfate formation, recombinant SULT1A3

substrate: serotonin


proof_lines: mutant enzyme Q56E/L67V/A101S/T117S/H213R/F222L/V243I/D249G, in 50 mM HEPES, pH 7.5, 7 mM MgCl2, and 1.5 mM dithiothreitol, at 22°C


proof_lines: mutant enzyme D249G, in 50 mM HEPES, pH 7.5, 7 mM MgCl2, and 1.5 mM dithiothreitol, at 22°C


proof_lines: mutant enzyme Q56E/A101S/T117S/Q177K/M223I/V243L, in 50 mM HEPES, pH 7.5, 7 mM MgCl2, and 1.5 mM dithiothreitol, at 22°C


substrate_name: rotigotine

substrate: rotigotine


proof_lines: heterodimer of mutants K266E and E275K, pH 7.4, 37°C


proof_lines: wild-type, initial rate value, pH 7.5, 25°C

proof_lines: wild type enzyme, in 50 mM HEPES, pH 7.5, 7 mM MgCl2, and 1.5 mM dithiothreitol, at 22°C


substrate_name: tapentadol

substrate: tapentadol


substrate_name: O-desmethyl tramadol

substrate: O-desmethyl tramadol


substrate_name: acetaminophen

substrate: acetaminophen


proof_lines: recombinant wild-type M-PST, pH 7.0, 37°C

proof_lines: recombinant wild-type P-PST, pH 7.0, 37°C


organism: Mus musculus

substrate_name: xanthurenic acid

assay_conditions_summary: pH 7.4; 310.15 K; buffer 50 mM Tris/HCl

proof_lines: isoform SULT1B1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C

substrate: xanthurenic acid


organism: Oryctolagus cuniculus


organism: Rattus norvegicus


organism: Squalius cephalus

substrate_name: genistein

substrate: genistein


proof_lines: with dopamine, isoenzyme A, pH 6.0, 37°C

proof_lines: with dopamine, isoenzyme B, pH 9.5, 37°C


substrate_name: daidzein

proof_lines: enzyme form SULT1A3, 4-sulfate formation

proof_lines: enzyme form SULT1A1, 7-sulfate formation

proof_lines: enzyme form SULT1E1, 7-sulfate formation

substrate: daidzein


proof_lines:
isoenzyme I
isoenzyme II
ST1A1


proof_lines: substrate inhibition, wild-type enzyme, enzyme form SULT1D1


proof_lines: substrate inhibition, mutant enzyme D247L, enzyme form SULT1D1


enzyme_display_name: chitinase

organism: Bacillus licheniformis

proof_lines:
wild-type, strain DSM13, pH 3.0, 37°C
wild-type, strain DSM8785, pH 3.0, 37°C
wild-type, strain DSM13, pH 6.0, 37°C

enzyme: chitinase


organism: Bacillus thuringiensis

substrate_name: colloidal chitin

proof_lines: mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C

substrate: colloidal chitin


organism: Candida albicans


organism: Chromobacterium sp.


organism: Clostridium paraputrificum


organism: Corallococcus sp. EGB


mutation_type: truncation

proof_lines: truncated protein lacking the CBD domain, pH 6, 50°C


proof_lines: truncated protein lacking both the CBD and FN3-2 domains, pH 6, 50°C


proof_lines: truncated protein lacking the FN3-2 domain, pH 6, 50°C


organism: Dioscorea polystachya

substrate_name: glycol chitin

substrate: glycol chitin


organism: Drosera rotundifolia

substrate_name: FITC-chitin

proof_lines: pH 5.2, recombinant protein lacking the 5 signal peptide, temperature not specified in the publication

proof_lines: recombinant protein lacking the thioredoxin tag, pH 5.2, temperature not specified in the publication

proof_lines: recombinant protein lacking the 5 signal peptide, pH 5.2, temperature not specified in the publication

substrate: FITC-chitin


organism: Ephemerocybe congregata


organism: Euphorbia characias

substrate_name: 4-nitrophenyl N-acetyl-D-glucosaminide

substrate: 4-nitrophenyl N-acetyl-D-glucosaminide


proof_lines:
wild-type, pH 2.2, 37°C
wild-type, pH 7.0, 37°C
wild-type, pH 4.6, 37°C


organism: Listeria innocua


organism: Macaca fascicularis

substrate_name: 4-methylumbelliferyl chitobioside

substrate: 4-methylumbelliferyl chitobioside


organism: Moritella marina

assay_conditions_summary: pH 5

proof_lines: pH 5.0, 0°C, purified recombinant enzyme

proof_lines: pH 5.0, 5°C, purified recombinant enzyme

proof_lines: pH 5.0, 15°C, purified recombinant enzyme


organism: Ostrinia furnacalis

proof_lines:
domain ChtII-C1, at pH 6.0 and 30°C
domain ChtII-C2, at pH 6.0 and 30°C
domain ChtII-C1C2, at pH 6.0 and 30°C


proof_lines: 30°C, pH not specified in the publication


organism: Paenibacillus barengoltzii


organism: Paenibacillus sp.

substrate_name: 4-nitrophenyl N,N'-diacetylchitobiose

substrate: 4-nitrophenyl N,N'-diacetylchitobiose


organism: Paenibacillus xylanexedens


organism: Penaeus vannamei

proof_lines:
pH 5.5, 25°C, presence of 0.6 M NaCl
pH 6.0, 25°C


organism: Pochonia chlamydosporia


organism: Pyrococcus furiosus


organism: Rhizomucor miehei


proof_lines:
mutant ChiADC-C, pH 4.5, 50Â°C
mutant ChiAD2L, pH 4.5, 50Â°C


organism: Saccharomyces cerevisiae


organism: Serratia marcescens

substrate_name: Unknown

substrate: Unknown


proof_lines:
pH 6.5, isozyme ChiC
pH 6.5, isozyme ChiB
pH 6.5, isozyme ChiA


substrate_name: beta-chitin

proof_lines: presence of lytic polysaccharide monooxygenase Cbp21, pH 8.0, 37°C

substrate: beta-chitin


proof_lines: presence of lytic polysaccharide monooxygenase Cbp21, pH 6.1, 37°C


organism: Streptomyces sp. DA11

substrate_name: powdered chitin

substrate: powdered chitin


organism: Trichoderma harzianum

proof_lines: pH 6.5, wild-type enzyme from Trichoderma harzianum

proof_lines: pH 6.5, wilde-type recombinant enzyme expressed in Escherichia coli


organism: Trichoderma virens


enzyme_display_name: orotidine-5'-phosphate decarboxylase

organism: Homo sapiens

substrate_name: orotidine 5'-phosphate

enzyme: orotidine-5'-phosphate decarboxylase

substrate: orotidine 5'-phosphate


organism: Methanobacterium thermoautotrophicus


organism: Methanothermobacter thermautotrophicus


organism: Plasmodium falciparum

proof_lines: monofunctional enzyme expressed in Escherichia coli

proof_lines: pH and temperature not specified in the publication, recombinant monofunctional enzyme, OMPDC activity

proof_lines: multienzyme complex of orotate phosphoribosyltransferase and orotidine 5-monophosphate decarboxylase


proof_lines:
pH 7.2, 25°C, D37A mutant
mutant D37A, pH 7.1, 25°C
pH 7.4, 25°C, D37A mutant


substrate_name: orotidine 5'-monophosphate

proof_lines:
wild type enzyme
pH 7.2, 25°C, wild-type enzyme
wild-type, pH 7.1, 25°C

substrate: orotidine 5'-monophosphate


proof_lines:
Q215A mutant
pH 7.1, 25°C, mutant Q215A
mutant Q215A, pH 7.1, 25°C


proof_lines:
pH 7.1, 25°C, mutant Y217F
mutant Y217F, pH 7.1, 25°C


substrate_name: 5-Fluoroorotidine 5'-phosphate

substrate: 5-Fluoroorotidine 5'-phosphate


proof_lines:
pH 7.1, 25°C, mutant R235A
mutant R235A, pH 7.1, 25°C


proof_lines:
mutant T100A, pH 7.1, 25°C
pH 7.2, 25°C, T100A mutant
pH 7.4, 25°C, T100A mutant


organism: Bombyx mori

proof_lines:
isoenzyme 1
isoenzyme 2


organism: Hexagrammos otakii

substrate_name: glycolchitin

proof_lines:
isoenzyme HoChiA
isoenzyme HoChiB
isoenzyme HoChiC

substrate: glycolchitin


organism: Scomber japonicus


organism: Unknown


organism: Danio rerio

substrate_name: L-thyroxine

substrate: L-thyroxine


enzyme_display_name: firefly luciferase

organism: Amydetes vivianii

substrate_name: D-firefly luciferin

assay_conditions_summary: pH 8; 295.15 K; cofactor ATP

enzyme: firefly luciferase

substrate: D-firefly luciferin


substrate_name: ATP

substrate: ATP


organism: Lamprigera yunnana

assay_conditions_summary: pH 7.5; 298.15 K; cofactor ATP


organism: Lampyris turkestanicus

substrate_name: D-luciferin

assay_conditions_summary: cofactor ATP

substrate: D-luciferin


assay_conditions_summary: pH 7.8; buffer 50 mM Tricine-NaOH; cofactor ATP

proof_lines: at pH 7.8, in 50 mM Tricine-NaOH buffer with 10 mM MgSO4

proof_lines: in 50 mM Tricine-NaOH buffer with 10 mM MgSO4, at pH 7.8


proof_lines:
mutant E354K/ins356K
mutant E354K


proof_lines:
mutant E354R
mutant E354R/ins356K


proof_lines: pH and temperature not specified in the publication, mutant enzyme L300E


proof_lines: pH and temperature not specified in the publication, mutant enzyme L300K


proof_lines: pH and temperature not specified in the publication, mutant enzyme L300R


organism: Luciola parvula

assay_conditions_summary: pH 8; 298.15 K; cofactor ATP


organism: Nipponoluciola cruciata

substrate_name: lauric acid

substrate: lauric acid


organism: Photinus pyralis

substrate_name: D-luciferyl-O-adenosine monophosphate

substrate: D-luciferyl-O-adenosine monophosphate


proof_lines:
pH 7.5, 25°C
native luciferase
recombinant luciferase


assay_conditions_summary: pH 7.4; cofactor ATP

proof_lines: mutant enzyme I432L, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2


assay_conditions_summary: pH 7.8; 298.15 K; buffer 25 mM glycylglycine; cofactor ATP

proof_lines: recombinant mutant enzyme V241I/F250T, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C


proof_lines: recombinant mutant enzyme V241I/F250G, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C


proof_lines: recombinant mutant enzyme G246A, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C


proof_lines: recombinant mutant enzyme V241I/F250S, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C


proof_lines: recombinant mutant enzyme F250S, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C


proof_lines: recombinant mutant enzyme G246A/F250G, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C


proof_lines: recombinant mutant enzyme V241I, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C


proof_lines: recombinant mutant enzyme V241I/G246A/F250T, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C


proof_lines: recombinant mutant enzyme V241I/G246A/F250S, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C


proof_lines: recombinant mutant enzyme G246A/F250S, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C


proof_lines: recombinant mutant enzyme F250T, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C


proof_lines: recombinant mutant enzyme G246A/F250T, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C


proof_lines: recombinant mutant enzyme F250G, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C


proof_lines: mutant enzyme D436G, in 50 mM Tris/HCl, at pH 7.4 and in the presence of 10 mM MgCl2

proof_lines: mutant enzyme D436G, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2


proof_lines: mutant enzyme I423L/D436G/L530R, in 50 mM Tris/HCl, at pH 7.4 and in the presence of 10 mM MgCl2


proof_lines: mutant enzyme I423L, in 50 mM Tris/HCl, at pH 7.4 and in the presence of 10 mM MgCl2


proof_lines: mutant enzyme L530R, in 50 mM Tris/HCl, at pH 7.4 and in the presence of 10 mM MgCl2

proof_lines: mutant enzyme L530R, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2


assay_conditions_summary: pH 7.4; 298.15 K; buffer 25 mM glycylglycine; cofactor ATP

proof_lines: wild type enzyme, in 50 mM Tris/HCl, at pH 7.4 and in the presence of 10 mM MgCl2

proof_lines: recombinant wild type enzyme, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C

proof_lines: wild type enzyme, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2


substrate_name: Luciferin

assay_conditions_summary: pH 7.8; buffer 0.02 M Tris-acetate; cofactor ATP

proof_lines: free enzyme, in 0.02 M Tris-acetate buffer, pH 7.8, containing 12 mM magnesium acetate, 0.2 mM EDTA, and 0.3 mM dithiothreitol

proof_lines: at pH 7.8, in 50 mM Tricine-NaOH buffer with 10 mM MgSO4

substrate: Luciferin


proof_lines:
mutant ins356R
mutant ins356Q
mutant ins356K


assay_conditions_summary: pH 7.8; 295.15 K; cofactor ATP

proof_lines: pH 7.8, 22°C, recombinant mutant R213K/T214N


assay_conditions_summary: pH 7.8; cofactor ATP

proof_lines: pH 7.8, temperature not specified in the publication, mutant enzyme H461D


proof_lines: pH 7.8, temperature not specified in the publication, mutant enzyme H489D


proof_lines: pH 7.8, temperature not specified in the publication, mutant enzyme H489M


proof_lines: pH 7.8, temperature not specified in the publication, mutant enzyme H489K


proof_lines: pH 7.8, 25°C, mutant enzyme I423L/D436G/L530R


proof_lines: 25°C, pH 7.8, mutant enzyme T214A/A215L/I232A/F295L/E345K

proof_lines: pH 7.8, 25°C, mutant enzyme T214A/A215L/I232A/F295L/E345K


assay_conditions_summary: pH 7.8; 298.15 K; cofactor ATP


organism: Photinus scintillans


proof_lines: pH 7.8, 22°C, recombinant wild-type enzyme


organism: Pyrocoelia sp. RF-2016

assay_conditions_summary: pH 7.5; cofactor ATP

proof_lines: pH 7.5, temperature not specified in the publication


enzyme_display_name: alcohol dehydrogenase (NADP+)

organism: Escherichia coli

substrate_name: NADP+; 1-Propanol

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

assay_conditions_summary: cofactor NADP+

enzyme: alcohol dehydrogenase (NADP+)

substrate: NADP+; 1-Propanol


organism: Homo sapiens

substrate_name: H+; NADPH; 4-Nitrobenzaldehyde

assay_conditions_summary: cofactor NADPH

substrate: H+; NADPH; 4-Nitrobenzaldehyde


substrate_name: H+; Succinate semialdehyde; NADPH

substrate: H+; Succinate semialdehyde; NADPH


organism: Lactobacillus brevis

substrate_name: 2-Butanone; NADPH; H+

substrate: 2-Butanone; NADPH; H+


organism: Mus musculus

substrate_name: NADPH; Benzaldehyde

substrate: NADPH; Benzaldehyde


organism: Mycobacterium bovis

substrate_name: H+; NADPH; Benzaldehyde

substrate: H+; NADPH; Benzaldehyde


organism: Mycobacterium smegmatis

substrate_name: H+; NADPH; Octanal

substrate: H+; NADPH; Octanal


organism: Rana perezi

substrate_name: NADPH; all-trans-Retinal; H+

substrate: NADPH; all-trans-Retinal; H+


organism: Rattus norvegicus


substrate_name: Methylglyoxal; NADPH; H+

substrate: Methylglyoxal; NADPH; H+


organism: Saccharomyces cerevisiae

substrate_name: H+; NADPH; Cinnamaldehyde

substrate: H+; NADPH; Cinnamaldehyde


organism: Sus scrofa

substrate_name: 4-Cyanobenzaldehyde; NADPH; H+

substrate: 4-Cyanobenzaldehyde; NADPH; H+


enzyme_display_name: cysteine synthase

organism: Bacillus anthracis

substrate_name: O-acetyl-L-serine

enzyme: cysteine synthase

substrate: O-acetyl-L-serine


proof_lines: production of H2S, mutant A72S, pH 8.2, 37°C


proof_lines: production of H2S, wild-type, pH 8.2, 37°C

proof_lines: wild-type, production of H2S, pH 8.2, 37°C


organism: Geobacillus stearothermophilus

substrate_name: L-cysteine

substrate: L-cysteine


organism: Glycine max

substrate_name: Sulfide

substrate: Sulfide


enzyme_display_name: 4-hydroxyphenylpyruvate dioxygenase

organism: Arabidopsis thaliana

proof_lines:
wild-type, pH 7,20°C
wild-type, pH 7, 25°C

enzyme: 4-hydroxyphenylpyruvate dioxygenase


proof_lines: recombinant His-tagged enzyme, pH 7.0, 30°C

proof_lines: 30°C, pH not specified in the publication


organism: Coptis japonica var. dissecta


mutation_type: deletion


proof_lines: pH 6.5, 37°C, mutant DELTAG388 and DELTAL385


proof_lines: pH 6.5, 37°C, wild-type enzyme and mutant DELTAG388


proof_lines: mutant lacking 12 N-terminal residues, pH 6.5, 37°C

proof_lines: mutant lacking 17 N-terminal residues, pH 6.5, 37°C


substrate_name: Unknown

proof_lines: pH 7.0, 30°C, recombinant His-tagged enzyme

substrate: Unknown


organism: Picrophilus oshimae


organism: Sphingobium sp.


organism: Haemophilus influenzae

proof_lines: 100 mM HEPES, pH 7.0, 1 microM enzyme, 20°C, steady state fluorescence titration

proof_lines: 100 mM HEPES, pH 8.0, 1 microM enzyme, 20°C, steady state fluorescence titration


proof_lines: wild type serine acetyltransferase motif, 100 mM HEPES, pH 7.0, 1 microM enzyme, 20°C, steady state fluorescence titration


organism: Lacticaseibacillus casei

substrate_name: O-acetyl-L-Ser

substrate: O-acetyl-L-Ser


organism: Leishmania donovani


organism: Leucaena leucocephala

substrate_name: hydrogen sulfide

proof_lines: pH 7.5, 22°C, recombinant enzymem, Michaelis-Menten model

proof_lines: pH 7.5, 22°C, recombinant enzyme, Lineweaver-Burke model

substrate: hydrogen sulfide


organism: Microcystis aeruginosa

proof_lines: recombinant enzyme, pH 7.0, 25°C, with DTT

proof_lines: recombinant enzyme, pH 7.0, 25°C, with glutathione, GSSG


organism: Mimosa pudica


organism: Oryza sativa Japonica Group


organism: Streptomyces lavendulae

proof_lines: 30°C, pH 8.0, cosubstrate: hydrogen sulfide


enzyme_display_name: non-specific serine/threonine protein kinase

substrate_name: ATP; L-Threonyl-AtSERK1

substrate: ATP; L-Threonyl-AtSERK1


substrate_name: Histone; ATP

enzyme: non-specific serine/threonine protein kinase

substrate: Histone; ATP


substrate_name: ATP; CRCK1

substrate: ATP; CRCK1


substrate_name: Protein; ATP

substrate: Protein; ATP


enzyme_display_name: corrinoid adenosyltransferase

organism: Homo sapiens

substrate_name: ATP

proof_lines: mutant S68F, in presence of 0.05 mM cob(I)alamin

enzyme: corrinoid adenosyltransferase

substrate: ATP


proof_lines: mutant C119Y, in presence of 0.05 mM cob(I)alamin


proof_lines: mutant G97E, in presence of 0.05 mM cob(I)alamin


proof_lines: mutant D218N, in presence of 0.05 mM cob(I)alamin


proof_lines:
native wild-type
N-terminally octa-His tagged wild-type
C-terminally octa-His tagged wild-type


proof_lines: mutant S126L, in presence of 0.05 mM cob(I)alamin


proof_lines: mutant H183Y, in presence of 0.05 mM cob(I)alamin


proof_lines: mutant D64G, in presence of 0.05 mM cob(I)alamin


proof_lines: mutant R76G, in presence of 0.05 mM cob(I)alamin


proof_lines: mutant F83S, in presence of 0.05 mM cob(I)alamin


proof_lines: mutant G63E, in presence of 0.05 mM cob(I)alamin


proof_lines: mutant K78R, in presence of 0.05 mM cob(I)alamin


proof_lines: mutant T161I, in presence of 0.05 mM cob(I)alamin


proof_lines: mutant G97R, in presence of 0.05 mM cob(I)alamin


organism: Listeria monocytogenes

proof_lines: mutant enzyme K98Q, pH and temperature not specified in the publication


substrate_name: cob(II)alamin

proof_lines: mutant enzyme D110N, pH and temperature not specified in the publication

substrate: cob(II)alamin


proof_lines: mutant enzyme T88V, pH and temperature not specified in the publication


proof_lines: mutant enzyme R200K, pH and temperature not specified in the publication


proof_lines: mutant enzyme E207Q, pH and temperature not specified in the publication


proof_lines: wild-type enzyme, pH and temperature not specified in the publication


organism: Salmonella enterica

substrate_name: cob(II)inamide

proof_lines: wild type enzyme, pH and temperature not specified in the publication

substrate: cob(II)inamide


organism: Thermoplasma acidophilum

substrate_name: hydroxocobalamin

assay_conditions_summary: pH 8; 343.15 K; cofactor ATP

proof_lines: pH 8.0, 70°C, mutant R124A, in presence of Mg2+

substrate: hydroxocobalamin


substrate_name: Ser/Thr peptide; ATP

substrate: Ser/Thr peptide; ATP


substrate_name: Peptide; ATP

substrate: Peptide; ATP


organism: Bacillus subtilis

substrate_name: ATP; SpoIIAA

substrate: ATP; SpoIIAA


organism: Caenorhabditis elegans

substrate_name: ATP; Peptide

substrate: ATP; Peptide


substrate_name: ATP; Kemptide

substrate: ATP; Kemptide


substrate_name: ATP; p65

substrate: ATP; p65


substrate_name: Myelin basic protein; ATP

substrate: Myelin basic protein; ATP


substrate_name: Myosin; ATP

substrate: Myosin; ATP


substrate_name: ATP; NFAT3

substrate: ATP; NFAT3


substrate_name: ATP; CASK CaM-kinase domain

substrate: ATP; CASK CaM-kinase domain


substrate_name: ATP; Sox-AKT-tide peptide

substrate: ATP; Sox-AKT-tide peptide


substrate_name: S6K1; Pi

substrate: S6K1; Pi


substrate_name: ATP; Protein

substrate: ATP; Protein


substrate_name: ATP; alpha-Casein

substrate: ATP; alpha-Casein


organism: Mus musculus


organism: Mycobacterium tuberculosis

substrate_name: EmbR; ATP

substrate: EmbR; ATP


substrate_name: ATP; GarA

substrate: ATP; GarA


organism: Oryza sativa

substrate_name: ATP; XA21K intracellular domain

substrate: ATP; XA21K intracellular domain


organism: Physarum polycephalum

substrate_name: ATP; Fragmin-actin complex

substrate: ATP; Fragmin-actin complex


organism: Rattus norvegicus

substrate_name: ATP; Protein tyrosine

substrate: ATP; Protein tyrosine


substrate_name: eIF-2alpha; ATP

substrate: eIF-2alpha; ATP


organism: Arabidopsis thaliana

substrate_name: diphosphate

substrate: diphosphate


substrate_name: UDP-glucose

substrate: UDP-glucose


substrate_name: alpha-D-glucose 1-phosphate

substrate: alpha-D-glucose 1-phosphate


substrate_name: dTTP

proof_lines:
pH 7.5, 25°C
cosubstrate: UTP, pH 7.5, 25°C
cosubstrate: dTTP, pH 7.5, 25°C

substrate: dTTP


substrate_name: UTP

proof_lines:
pH 7.5, 25°C
cosubstrate: UTP, pH 7.5, 25°C

substrate: UTP


organism: Cucumis melo

substrate_name: UDP-galactose

substrate: UDP-galactose


organism: Danio rerio


organism: Drosophila melanogaster


organism: Giardia duodenalis

proof_lines: cosubstrate alpha-D-galactose 1-phosphate, S0.5 value, hyperbolic behaviour, pH 8.0, 37°C

proof_lines: cosubstrate TTP, S0.5 value, hyperbolic behaviour, pH 8.0, 37°C


organism: Gracilaria chouae

proof_lines: pH 7.5, temperature not specified in the publication


organism: Homo sapiens

proof_lines: pH 7.5, 37°C, recombinant mutant S309N/S311R


proof_lines: pH 7.5, 37°C, recombinant mutant P414G/T415P


proof_lines: pH 7.5, 37°C, recombinant mutant T406K/M407L


proof_lines: pH 7.5, 37°C, recombinant mutant N491P/L492E


organism: Hordeum vulgare


proof_lines:
recombinant wild-type, pH 7.6, 25°C
wild-type protein


proof_lines:
Ccut-8 mutant protein
Ccut-32 mutant protein
Ncut-21 mutant protein


mutation_signature: Δ1

proof_lines:
Delta 1-8 mutant protein
Delta 1-4 mutant protein


organism: Leishmania major


organism: Nostoc sp.


organism: Saccharina japonica


organism: Saccharomyces cerevisiae


organism: Streptococcus equi


organism: Sulfurisphaera tokodaii


organism: Synechocystis sp.


organism: Thermocrispum agreste


organism: Trypanosoma brucei

proof_lines: pH 7.2, temperature not specified in the publication, recombinant enzyme


organism: Xanthomonas campestris pv. campestris


organism: Xanthomonas citri

substrate_name: TTP

substrate: TTP


enzyme_display_name: subtilisin

organism: Bacillus licheniformis

proof_lines: pH 8.0, 25°C, recombinant wild-type enzyme

proof_lines: recombinant wild-type enzyme, pH 7.5, 30°C

enzyme: subtilisin


enzyme_display_name: aralkylamine N-acetyltransferase

organism: Antheraea pernyi

substrate_name: acetyl-CoA

assay_conditions_summary: pH 7.4; 310.15 K; cofactor CoA

enzyme: aralkylamine N-acetyltransferase

substrate: acetyl-CoA


organism: Bombyx mori

substrate_name: tryptamine

assay_conditions_summary: cofactor CoA

substrate: tryptamine


organism: Bos taurus


organism: Chlamydomonas reinhardtii

substrate_name: serotonin

substrate: serotonin


organism: Danio rerio


organism: Drosophila melanogaster

assay_conditions_summary: pH 8; cofactor CoA

proof_lines: pH 8.0, temperature not specified in the publication


organism: Homo sapiens


organism: Mycobacterium tuberculosis

substrate_name: histamine

assay_conditions_summary: pH 7; 298.15 K; cofactor CoA

proof_lines:
cosubstrate acetyl-CoA, pH 7.0, 25°C
cosubstrate histamine, pH 7.0, 25°C

substrate: histamine


organism: Mycolicibacterium smegmatis

substrate_name: propionyl-CoA

proof_lines:
cosubstrate tyramine, pH 7.0, 25°C
cosubstrate acetyl-CoA, pH 7.0, 25°C

substrate: propionyl-CoA


organism: Oryza sativa


organism: Ovis aries

proof_lines: pH 6.8, 37°C, recombinant mutant I57A/V59A


substrate_name: Unknown

substrate: Unknown


proof_lines: above, pH 6.8, 37°C, recombinant mutants P64A, P64G, P64W, and recombinant truncation mutants


proof_lines: pH 6.8, 37°C, recombinant wild-type enzyme


organism: Rattus norvegicus

assay_conditions_summary: pH 6.8; 310.15 K; cofactor CoA


organism: Sparus aurata

assay_conditions_summary: pH 6.8; 300.15 K; cofactor CoA


organism: Sus scrofa


organism: Tribolium castaneum

substrate_name: decanoyl-CoA

assay_conditions_summary: pH 8; 310.15 K; cofactor CoA

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: decanoyl-CoA


proof_lines: pH 8.0, 25°C, recombinant mutant P210G/T211G/N212G


proof_lines: pH 8.0, 25°C, recombinant mutant P210G/T211G


proof_lines: recombinant mutant T58A/G165Y/L216W, pH 7.5, 30°C


proof_lines: recombinant mutant T58A/L216W, pH 7.5, 30°C


proof_lines: recombinant mutant T58A/G165L/L216W, pH 7.5, 30°C


proof_lines: recombinant mutant T58A/G165I/L216W, pH 7.5, 30°C


organism: Bacillus subtilis

proof_lines: pH 8.5, 37°C, recombinant mutant S62I/A153V/G166S/I205V, in presence of 2 M GdmCl

proof_lines: pH 8.5, 37°C, recombinant mutant S62I/A153V/G166S/I205V, in absence of GdmCl or SDS

proof_lines: pH 8.5, 37°C, recombinant mutant S62I/A153V/G166S/I205V, in presence of SDS


proof_lines: pH 8.5, 37°C, recombinant mutant S62I, in presence of SDS

proof_lines: pH 8.5, 37°C, recombinant mutant S62I, in absence of GdmCl or SDS


proof_lines: pH 8.5, 37°C, recombinant mutant S62I/G166M, in presence of SDS

proof_lines: pH 8.5, 37°C, recombinant mutant S62I/G166M, in presence of 2 M GdmCl

proof_lines: pH 8.5, 37°C, recombinant mutant S62I/G166M, in absence of GdmCl or SDS


proof_lines: pH 8.5, 37°C, recombinant mutant G166M, in presence of SDS

proof_lines: pH 8.5, 37°C, recombinant mutant G166M, in presence of 2 M GdmCl

proof_lines: pH 8.5, 37°C, recombinant mutant G166M, in absence of GdmCl or SDS


proof_lines: pH 8.5, 37°C, recombinant wild-type enzyme, in presence of 2 M GdmCl

proof_lines: pH 8.5, 37°C, recombinant wild-type enzyme, in absence of GdmCl or SDS

proof_lines: pH 8.5, 37°C, recombinant wild-type enzyme, in presence of SDS


proof_lines:
recombinant enzyme, 5°C, pH 8.6
recombinant enzyme, 45°C, pH 8.6
recombinant enzyme, 25°C, pH 8.6


substrate_name: casein

proof_lines: pH 10.0, 80°C, recombinant enzyme, calculated by BRENDA

substrate: casein


organism: Shewanella frigidimarina


organism: Shouchella clausii

proof_lines: synthetic peptide corresponding to the N-terminal extension behaves as a mixed noncompetitive inhibitor of active ISP, pH and temperature not specified in the publication


organism: Aspisoma lineatum

substrate_name: ATP

assay_conditions_summary: pH 8; cofactor ATP

proof_lines: pH 8.0, temperature not specified in the publication, isoenzyme AL1

proof_lines: pH 8.0, temperature not specified in the publication, isoenzyme AL2

substrate: ATP


organism: Unknown

substrate_name: D-luciferin

substrate: D-luciferin


substrate_name: cob(I)alamin

assay_conditions_summary: cofactor ATP

substrate: cob(I)alamin


organism: Allium tuberosum

substrate_name: O-acetyl-L-Ser

substrate: O-acetyl-L-Ser


organism: Allochromatium vinosum

substrate_name: O-acetyl-L-serine

proof_lines:
pH 7.4, 37°C, isoenzyme 2
pH 7.4, 37°C, isoenzyme 1
pH 7.4, 37°C, enzyme 1

substrate: O-acetyl-L-serine


organism: Arabidopsis sp.

proof_lines:
pH 7.5, 37°C, isoenzyme A
pH 7.5, 37°C, isoenzyme C
pH 7.5, 37°C, isoenzyme B


organism: Chlamydomonas reinhardtii

proof_lines:
pH 7.5, 50°C
pH 7.5, 50°C, isoenzyme 1 and 2
pH 7.5, 50 C


organism: Citrullus lanatus

proof_lines:
pH 8.0, 30°C, isoenzyme 1
pH 8.0, 30°C, isoenzyme 2


organism: Lathyrus oleraceus

proof_lines:
pH 7.8, 35°C, isoenzyme 2
pH 7.8, 35°C
pH 8.0, 30°C, isoenzyme 2


organism: Rhodocyclus tenuis

proof_lines:
isoenzyme 1
isoenzyme 1 and 2
isoenzyme 2


organism: Spinacia oleracea

proof_lines:
pH 7.5, 25°C, chloroplast enzyme
pH 8.0, 30°C
pH 8.0, 25°C, isoenzyme 3


organism: Synechococcus sp.


organism: Xanthium pennsylvanicum

proof_lines:
,isoenzyme 3
isoenzymes 1 and 2
,isoenzyme 2


organism: Sphingomonas elodea

proof_lines:
isoenzyme UgpG, 30°C, pH 7.8
isoenzyme RmlA, 30°C, pH 7.8


organism: Arabidopsis thaliana

substrate_name: Sulfide

substrate: Sulfide


organism: Photinus pyralis


organism: Salmonella enterica

assay_conditions_summary: pH 8; 298.15 K; cofactor ATP

proof_lines: pH 8.0, 25°C, recombinant CobA mutant F91W/W93F


proof_lines: pH 8.0, 25°C, recombinant CobA mutant W93A


proof_lines: pH 8.0, 25°C, recombinant CobA mutant W93F


proof_lines: pH 8.0, 25°C, recombinant CobA mutant F91A


enzyme_display_name: thymidylate synthase

organism: Cryptococcus neoformans

enzyme: thymidylate synthase


organism: Enterococcus faecalis

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Escherichia coli


proof_lines: 10fold higher than wild-type thymidylate synthase

proof_lines: 30fold higher than wild-type thymidylate synthase


substrate_name: methylenetetrahydropteroylgluamate

substrate: methylenetetrahydropteroylgluamate


substrate_name: methylenetetrahydrofolate

substrate: methylenetetrahydrofolate


substrate_name: dUMP

substrate: dUMP


organism: Homo sapiens


proof_lines:
wild type enzyme, at 37°C
wild type


proof_lines:
presence of Mg2+, pH 7.5, 22°C
pH 7.5, 22°C
pH 7.2, 22°C


organism: Mycobacterium tuberculosis


organism: Penaeus vannamei


organism: Plasmodium falciparum


proof_lines: Ala-FACE mutant enzyme with residues 284, 285, 288, 289, and 292 mutated to Ala


organism: Pneumocystis carinii


organism: Shrimp white spot syndrome virus


organism: Trypanosoma brucei


enzyme_display_name: kynurenine--oxoglutarate transaminase

organism: Aedes aegypti

substrate_name: L-asparagine

enzyme: kynurenine--oxoglutarate transaminase

substrate: L-asparagine


organism: Haemaphysalis longicornis

assay_conditions_summary: pH 7.5; 310.15 K; buffer 200 mM phosphate

proof_lines: in 200 mM phosphate buffer, pH 7.5, at 37°C

proof_lines: in 200 mM phosphate buffer, pH7.5, at 37°C


assay_conditions_summary: pH 7.4; 310.15 K; buffer 100 mM potassium phosphate

proof_lines: in 100 mM potassium phosphate buffer (pH 7.4), at 37°C

proof_lines: mass spectrometry assay, pH 7.5, temperature not specified in the publication

proof_lines: pH and temperature not specified in the publication


substrate_name: L-kynurenine

substrate: L-kynurenine


organism: Mus musculus

substrate_name: L-cysteine

assay_conditions_summary: pH 9; 318.15 K; buffer 100 mM boric acid

proof_lines: in 100 mM boric acid buffer, pH 9.0, at 45°C

substrate: L-cysteine


substrate_name: hydroxyphenylpyruvate

substrate: hydroxyphenylpyruvate


organism: Rattus norvegicus

substrate_name: pyruvate

substrate: pyruvate


proof_lines: pH 8.0, 37°C, recombinant mitochondrial enzyme


proof_lines: pH 8.0, 37°C, recombinant enzyme, cosubstrate L-kyrunenine

proof_lines: pH 8.0, 37°C, alpha-aminoadipate aminotransferase activity


organism: Unknown


organism: Arabidopsis thaliana


organism: Photinus pyralis

proof_lines:
mutant enzyme G246A
pH 8.6, mutant enzyme G246A


organism: Bos taurus

substrate_name: Inosine

substrate: Inosine


organism: Avena sativa


organism: Limosilactobacillus reuteri

substrate_name: cob(II)alamin

proof_lines:
mutant F112Y, Co2+ assay
mutant F112Y, Co+ assay

substrate: cob(II)alamin


proof_lines:
wild-type, Co+ assay
wild-type, Co2+ assay


proof_lines:
mutant F187A, Co2+ assay
mutant F187A, Co+ assay


organism: Salmonella enterica

proof_lines: pH 8.0, 25°C, recombinant CobA mutant F91Y


proof_lines: pH 8.0, 25°C, recombinant CobA mutant W93Y


proof_lines: pH 8.0, 25°C, recombinant CobA mutant F91W


proof_lines: pH 8.0, 25°C, recombinant CobA mutant F91H


organism: Escherichia coli

substrate_name: orotidine 5'-phosphate

substrate: orotidine 5'-phosphate


proof_lines:
mutant enzyme F250S
pH 8.6, mutant enzyme F250S


organism: Drosophila melanogaster

assay_conditions_summary: pH 8; 295.15 K; cofactor CoA

proof_lines: cosubstrate hexanoyl-CoA, wild-type, pH 8.0, 22°C

proof_lines: cosubstrate butanoyl-CoA, wild-type, pH 8.0, 22°C


substrate_name: acetyl-CoA

substrate: acetyl-CoA


substrate_name: histamine

substrate: histamine


substrate_name: cob(I)alamin

substrate: cob(I)alamin


substrate_name: ATP

substrate: ATP


proof_lines: recombinant mutant Q358E, pH and temperature not specified in the publication


proof_lines: recombinant mutant N261D, pH and temperature not specified in the publication


proof_lines: recombinant mutant Q286E, pH and temperature not specified in the publication


proof_lines: recombinant mutant S246A, pH and temperature not specified in the publication


substrate_name: tryptamine

substrate: tryptamine


organism: Vibrio harveyi

substrate_name: chitin

proof_lines: pH 5.5, 37°C, colloidal chitin, mutant enzym W70A

substrate: chitin


proof_lines: pH 5.5, 37°C, colloidal chitin, mutant enzym W231F


substrate_name: chitohexaose

proof_lines: pH 5.5, 37°C, colloidal chitin, mutant enzym W231A

substrate: chitohexaose


proof_lines: pH 5.5, 37°C, colloidal chitin, mutant enzym S33W


organism: Bacillus amyloliquefaciens

substrate_name: Unknown

substrate: Unknown


organism: Mycobacterium tuberculosis

substrate_name: indole

substrate: indole


organism: Streptomyces avermitilis


proof_lines: pH 7.0, 25°C, wild-type enzyme and mutant N245I


organism: Homo sapiens

substrate_name: dUMP

proof_lines: apparent value, mutant enzyme V3F, pH not specified in the publication, at 37°C

substrate: dUMP


proof_lines: apparent value, mutant enzyme V3L, pH not specified in the publication, at 37°C


proof_lines: apparent value, mutant enzyme V3Y, pH not specified in the publication, at 37°C


proof_lines: apparent value, mutant enzyme V3T, pH not specified in the publication, at 37°C


organism: Niallia circulans

substrate_name: carboxymethyl chitin

substrate: carboxymethyl chitin


organism: Dichelobacter nodosus


organism: Saccharomyces cerevisiae


substrate_name: 1-(beta-D-erythrofuranosyl)orotic acid

substrate: 1-(beta-D-erythrofuranosyl)orotic acid


substrate_name: D-luciferin

proof_lines: mutant I423L, in the presence of 0.01 mM ATP

proof_lines: mutant I423L, in the presence of 0.001 mM D-luciferin

substrate: D-luciferin


proof_lines: mutant I423L/D436G/L530R, in the presence of 0.001 mM D-luciferin

proof_lines: mutant I423L/D436G/L530R, in the presence of 0.01 mM ATP


proof_lines: mutant D436G, in the presence of 0.01 mM ATP

proof_lines: mutant D436G, in the presence of 0.001 mM D-luciferin


proof_lines: mutant L530R, in the presence of 0.01 mM ATP

proof_lines: mutant L530R, in the presence of 0.001 mM D-luciferin


substrate_name: Luciferin

substrate: Luciferin


proof_lines:
mutant F112W, Co+ assay
mutant F112W, Co2+ assay


proof_lines:
mutant DELTAS183, Co2+ assay
mutant DELTAS183, Co+ assay


proof_lines:
mutant F163A, Co2+ assay
mutant F163A, Co+ assay


proof_lines:
mutant F112H, Co2+ assay
mutant F112H, Co+ assay


proof_lines: pH 8.0, 25°C, recombinant CobA mutant W93H


proof_lines: pH 8.0, 25°C, recombinant CobA mutant W93D


proof_lines: pH 8.0, 25°C, recombinant CobA mutant F91A/W93D


proof_lines: pH 8.0, 25°C, recombinant wild-type CobA

proof_lines: pH 8.0, 37°C, recombinant full length wild-type enzyme


proof_lines: SULT1A3 double mutant H143Y/E146A, pH 7.4, 37°C


proof_lines:
wild-type SULT1A3, pH 7.4, 37°C
wild-type SULT1A1, pH 7.4, 37°C
wild-type SULT1A1


organism: Lederbergia lenta

substrate_name: succinyl-AAPF-p-nitroanilide

proof_lines: pH 7.5, 25°C mutant S166C-S-d and M222C-S-d

substrate: succinyl-AAPF-p-nitroanilide


proof_lines:
pH 7.5, 25°C mutant M222C-S-f
pH 7.5, 25°C mutant M222C-S-e
pH 7.5, 25°C mutant M222C-S-a


proof_lines:
pH 7.5, 25°C mutant S156C-S-c
pH 7.5, 25°C mutant S156C-S-e
pH 7.5, 25°C mutant S156C-S-f


proof_lines: pH 7.5, 25°C mutant S156C-S-c and S166C-S-f


organism: Alcaligenes faecalis

substrate_name: benzylpenicillin

proof_lines: pH 7.5, 25°C, mutant enzyme T206G and wild-type enzyme

substrate: benzylpenicillin


proof_lines: pH 7.5, 25°C, mutant enzyme T206G//S213G


proof_lines: pH 7.5, 25°C, mutant enzyme T206G/S213G/T219G


substrate_name: orotidine 5'-phosphate

substrate: orotidine 5'-phosphate


organism: Photinus pyralis


assay_conditions_summary: pH 8.5; 308.15 K; cofactor ATP


assay_conditions_summary: pH 8; 308.15 K; cofactor ATP


assay_conditions_summary: pH 8.5; 298.15 K; cofactor ATP


organism: Pyrophorus plagiophthalamus

substrate_name: luciferyl-O-adenosine monophosphate

substrate: luciferyl-O-adenosine monophosphate


organism: Lacticaseibacillus casei


proof_lines:
mutant TS N229D
mutant TS N229D, dCMP as substrate


substrate_name: histamine

substrate: histamine


organism: Limosilactobacillus reuteri

proof_lines: mutant D35R/R128D, at subsaturating concentrations of 30 mM ATP and 0.02 mM cob(I)alamin

proof_lines: mutant D35R/R128D, at subsaturating concentrations of ATP 30 mM ATP and 0.02 mM cob(I)alamin


substrate_name: O-acetyl-L-serine

substrate: O-acetyl-L-serine


organism: Bos taurus

substrate_name: Inosine

substrate: Inosine


organism: Hevea brasiliensis


proof_lines:
30°C, pH 4.2, purified wild-type
30°C, pH 4.2, recombinant wild-type


organism: Oryza sativa

substrate_name: reduced N-acetylchitopentaose

substrate: reduced N-acetylchitopentaose


organism: Vibrio harveyi

substrate_name: p-nitrophenyl-N,N'-diacetylchitobiose

substrate: p-nitrophenyl-N,N'-diacetylchitobiose


enzyme_display_name: (S)-hydroxynitrile lyase

organism: Baliospermum montanum

substrate_name: 1-naphthalene carboxaldehyde

enzyme: (S)-hydroxynitrile lyase

substrate: 1-naphthalene carboxaldehyde


substrate_name: (R)-mandelonitrile

substrate: (R)-mandelonitrile


proof_lines: pH 4.23, 25°C, mutant enzyme H103C/N156G


proof_lines: pH 4.23, 25°C, mutant enzyme H103C/N156D


assay_conditions_summary: pH 5

proof_lines: mutant enzyme F125T/Y133F, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme F125T/Y133F


proof_lines: mutant enzyme L121Y/F125T/Y133F, pH and temperature not specified in the publication


substrate_name: mandelonitrile

proof_lines: mutant enzyme F125T, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme F125T

substrate: mandelonitrile


proof_lines: mutant enzyme L121Y, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121Y


proof_lines: mutant enzyme V106F/L121Y/F125T, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y/F125T


proof_lines: mutant enzyme L146M, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L146M


proof_lines: mutant enzyme I209A, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme I209A


substrate_name: rac-mandelonitrile

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121A

substrate: rac-mandelonitrile


enzyme_display_name: carnitine O-palmitoyltransferase

substrate_name: L-carnitine

assay_conditions_summary: cofactor CoA

enzyme: carnitine O-palmitoyltransferase

substrate: L-carnitine


substrate_name: palmitoyl-CoA

substrate: palmitoyl-CoA


proof_lines: P504L/V605L mutant protein, COS-7 cell lysate


proof_lines: F352C/V368I mutant protein, COS-7 cell lysate


proof_lines: F352C/V368I/V605L mutant protein, COS-7 cell lysate


assay_conditions_summary: pH 7.4; 310.15 K; cofactor CoA


assay_conditions_summary: pH 7.4; 298.15 K; cofactor CoA

proof_lines: mutant enzyme Y479F, in presence of 0.25 mM cardiolipin, at pH 7.4 and 25°C

proof_lines: mutant enzyme Y479F, in absence of cardiolipin, at pH 7.4 and 25°C


proof_lines: mutant enzyme S113L, in absence of cardiolipin, at pH 7.4 and 25°C

proof_lines: mutant enzyme S113L, in presence of 0.25 mM cardiolipin, at pH 7.4 and 25°C


proof_lines: mutant enzyme P50H, in presence of 0.25 mM cardiolipin, at pH 7.4 and 25°C

proof_lines: mutant enzyme P50H, in absence of cardiolipin, at pH 7.4 and 25°C


proof_lines: wild type enzyme, in absence of cardiolipin, at pH 7.4 and 25°C

proof_lines: wild type enzyme, in presence of 0.25 mM cardiolipin, at pH 7.4 and 25°C

proof_lines: wild-type protein and P504L/V605L mutant protein, COS-7 cell lysate (doubly transfected)


organism: Larimichthys crocea

proof_lines: 25°C, pH not specified in the publication


organism: Ovis aries

proof_lines: chimaera in which the distinctive N-terminal segment of ovine m-BPT 1 is replaced with that from rat M-CPT 1


proof_lines:
wild-type L-CPT 1
wild-type L-CPT1


proof_lines:
wild-type M-CPT 1
wild-type M-CPT1


organism: Rattus norvegicus


proof_lines:
wild-type
wild-type enzyme


organism: Sus scrofa

assay_conditions_summary: pH 7; cofactor CoA


organism: Tachysurus fulvidraco

proof_lines:
enzyme from muscle, at pH 8.0 and 37°C
enzyme from heart, at pH 8.0 and 37°C
enzyme from spleen, at pH 8.0 and 37°C


proof_lines: mutant enzyme L121Y/L146M, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/L146M


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121F


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121W


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121S


proof_lines: mutant enzyme V106F/F125T, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme V106F/F125T


proof_lines: mutant enzyme V106F/L146M, pH and temperature not specified in the publication


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121C


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121Q


proof_lines: mutant enzyme I209G, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme I209G


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121T


proof_lines: wild type enzyme, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, wild-type enzyme


proof_lines: mutant enzyme L121Y/F125T, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T


proof_lines: mutant enzyme Y133F, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme Y133F


proof_lines: mutant enzyme F125T/L146M, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme F125T/L146M


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121V


proof_lines: mutant enzyme L152F, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L152F


proof_lines: mutant enzyme L148F, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L148F


enzyme_display_name: phosphoenolpyruvate carboxykinase (GTP)

organism: Homo sapiens

substrate_name: GDP

proof_lines: Mg2+ concentration 5 mM, Y235S mutant. Vmax = 10 micromol/min/mg

proof_lines: Mg2+ concentration 7.5 mM, Y235S mutant. Vmax = 11 micromol/min/mg

enzyme: phosphoenolpyruvate carboxykinase (GTP)

substrate: GDP


proof_lines: Mg2+ concentration 2 mM, wild-type. Vmax = 33 micromol/min/mg

proof_lines: Mn2+ concentration 1.5 mM, Mg2+ concentration 2 mM, wild-type. Vmax = 31 micromol/min/mg


proof_lines: Mg2+ concentration 5 mM, Y235F mutant. Vmax = 3 micromol/min/mg

proof_lines: Mg2+ concentration 7.5 mM, Y235F mutant. Vmax = 3 micromol/min/mg


proof_lines: Mg2+ concentration 5 mM, Y235A mutant. Vmax = 15 micromol/min/mg

proof_lines: Mg2+ concentration 2 mM, Y235A mutant. Vmax = 15 micromol/min/mg

proof_lines: Mg2+ concentration 7.5 mM, Y235A mutant. Vmax = 15 micromol/min/mg


organism: Mycolicibacterium smegmatis

substrate_name: phosphoenolpyruvate

proof_lines:
pH 7.2, 37°C
pH 7.2, 37°C, bicarbonate

substrate: phosphoenolpyruvate


substrate_name: oxaloacetate

substrate: oxaloacetate


enzyme_display_name: phosphoenolpyruvate carboxylase

organism: Alternanthera ficoidea

proof_lines:
+ 5 mM D-glucose 6-phosphate
without 5 mM D-glucose 6-phosphate

enzyme: phosphoenolpyruvate carboxylase


organism: Alternanthera pungens


organism: Alternanthera sessilis

proof_lines:
+ 5 mM glucose 6-phosphate
without 5 mM glucose 6-phosphate


organism: Clostridium perfringens

assay_conditions_summary: pH 7.2; 310.15 K; buffer 50 mM HEPES-NaOH

proof_lines: in the presence of 2 mM Mg2+, in 50 mM HEPES-NaOH buffer (pH 7.2), at 37°C


organism: Corynebacterium glutamicum


organism: Escherichia coli


proof_lines: wild type enzyme, and mutant enzyme Arg703Gly/Arg704Gly


organism: Flaveria pringlei


organism: Flaveria trinervia


organism: Nostoc sp. PCC 7120 = FACHB-418


organism: Oryza sativa Japonica Group

proof_lines: K119EA mutant, presence of glucose 6-phosphate, S0.5 value, Hill coefficient 2.00, pH 7.3, 30°C

proof_lines: N-terminal replacement mutant, S0.5 value, Hill coefficient 1.77, pH 7.3, 30°C


proof_lines: wild-type, S0.5 value, Hill coefficient 1.52, pH 7.3, 30°C

proof_lines: wild-type, S0.5 value, Hill coefficient 1.63, pH 8.0, 30°C


organism: Pyrococcus furiosus

proof_lines: 50°C, pH not specified in the publication


organism: Ricinus communis

assay_conditions_summary: pH 8

proof_lines:
at pH 8.0
at pH 7.3


organism: Saccharolobus solfataricus


organism: Selenicereus undatus


organism: Sorghum bicolor

proof_lines: DELTAC4, deletion of the last 4 c-terminal amino acids


proof_lines: DELTAC1, deletion of the last c-terminal amino acid


organism: Synechocystis sp. PCC 6803


proof_lines:
presence of K+, pH 8.0, 28°C
presence of Na+, pH 8.0, 28°C


organism: Thermostichus vulcanus

substrate_name: Unknown

substrate: Unknown


organism: Zea mays


organism: Rattus norvegicus

proof_lines: mutant enzyme A467G, in 50 mM HEPES pH 7.5, 10 mM dithiothreitol, 4 mM MgCl2, at 25°C


substrate_name: GTP

proof_lines: wild type enzyme, in 50 mM HEPES pH 7.5, 10 mM dithiothreitol, 4 mM MgCl2, at 25°C

substrate: GTP


organism: Thermococcus kodakarensis


organism: Hevea brasiliensis

proof_lines: mutant enzyme F210I, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme F210I


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121M


proof_lines: mutant enzyme V106F, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme V106F


proof_lines: mutant enzyme L121Y/F125T/L146M, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T/L146M


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121H


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121I


proof_lines: mutant enzyme C81L, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme C81L


proof_lines: mutant enzyme V106F/L121Y, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y


substrate_name: acetone cyanohydrin

proof_lines:
pH 5.4
isoform HNL, at pH 7.2 and 25°C

substrate: acetone cyanohydrin


organism: Manihot esculenta

substrate_name: (S)-mandelonitrile

substrate: (S)-mandelonitrile


assay_conditions_summary: pH 5; 296.15 K; buffer pH above 6

proof_lines: wild type enzyme, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication

proof_lines: wild type enzyme, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication


substrate_name: benzaldehyde

assay_conditions_summary: pH 5; buffer pH 5

proof_lines: mutant enzyme K176P/K199P/K224P, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication

proof_lines: mutant enzyme K176P/K199P/K224P, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication

substrate: benzaldehyde


proof_lines: mutant enzyme H103Y, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication


assay_conditions_summary: pH 5; buffer pH above 6

proof_lines: mutant enzyme H103L, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication

proof_lines: mutant enzyme H103L, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication


proof_lines: mutant enzyme H103M, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication

proof_lines: mutant enzyme H103M, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication


proof_lines: mutant enzyme K176P, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication

proof_lines: mutant enzyme K176P, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication


substrate_name: 4-biphenyl carboxaldehyde

proof_lines:
pH 4.2, 22°C
pH 7.5
recombinant enzyme

substrate: 4-biphenyl carboxaldehyde


organism: Corynebacterium glutamicum


organism: Homo sapiens

assay_conditions_summary: pH 8; 303.15 K; cofactor CoA

proof_lines: 30°C, recombinant wildtype isozyme CPT1A

proof_lines: pH 8.0, 37°C, recombinant wild-type enzyme


organism: Unknown


enzyme_display_name: arylamine N-acetyltransferase

proof_lines: using control cholangiocarcinoma KKU-100 cells after treatment with the mixture of inflammatory cytokines for 48 h, Vmax: 135.7 pmol/min/mg. A significant decrease in Vmax is shown but without affecting Km, implying a change in the amount o

enzyme: arylamine N-acetyltransferase


organism: Arabidopsis thaliana

proof_lines: dephosphorylated isozyme PPC1, at pH 7.3


proof_lines:
pH 8.0
isoenzyme PC-I
isoenzyme PC-II


enzyme_display_name: glucose-6-phosphate isomerase

organism: Thermococcus litoralis

substrate_name: D-Glucose 6-phosphate

enzyme: glucose-6-phosphate isomerase

substrate: D-Glucose 6-phosphate


organism: Thermoplasma acidophilum

substrate_name: D-Fructose 6-phosphate

substrate: D-Fructose 6-phosphate


organism: Trypanosoma brucei


organism: Mycobacterium tuberculosis


organism: Oryctolagus cuniculus

substrate_name: D-Mannose 6-phosphate

substrate: D-Mannose 6-phosphate


organism: Plasmodium falciparum


organism: Pyrobaculum aerophilum


organism: Pyrococcus furiosus


organism: Rhizobium meliloti


organism: Saccharomyces cerevisiae


organism: Spinacia oleracea


organism: Aeropyrum pernix


organism: Aspergillus niger


organism: Bos taurus


organism: Entamoeba histolytica


organism: Escherichia coli


organism: Geobacillus stearothermophilus


organism: Homo sapiens


organism: Lactococcus lactis subsp. lactis


organism: Methanocaldococcus jannaschii


organism: Mus musculus


enzyme_display_name: glutathione-disulfide reductase

organism: Chlamydomonas reinhardtii

substrate_name: GSSG

assay_conditions_summary: cofactor NADPH

proof_lines:
isozmes GR-1 and GR-2
isozyme GR-2
isozyme GR-1

enzyme: glutathione-disulfide reductase

substrate: GSSG


organism: Picea rubens

proof_lines:
isozyme GR-1NH
isozyme GR-1H
isozyme GR-2NH


organism: Unknown

substrate_name: NADPH

substrate: NADPH


organism: Badhamia polycephala

substrate_name: glutathione disulfide

substrate: glutathione disulfide


organism: Cenchrus americanus

assay_conditions_summary: pH 8; cofactor NADPH

proof_lines: pH 8.0, temperature not specified in the publication


proof_lines: recombinant glutathione-trypanothione reductase-like enzyme


organism: Populus trichocarpa

assay_conditions_summary: pH 7.6; 295.15 K; cofactor NADPH


organism: Saccharomyces cerevisiae

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NADPH

proof_lines:
native Glr1
recombinant Glr1
pH 7.5, 37°C


organism: Xanthomonas campestris

substrate_name: NADH

substrate: NADH


substrate_name: trypanothione

proof_lines: pH 7.5, mutant enzyme A18E/N21W/R22N/N101D/K105D/R319A

substrate: trypanothione


enzyme_display_name: carbonyl reductase (NADPH)

organism: Caenorhabditis elegans

substrate_name: H+; NADPH; Isatin

enzyme: carbonyl reductase (NADPH)

substrate: H+; NADPH; Isatin


substrate_name: 4-Oxonon-2-enal; NADPH

substrate: 4-Oxonon-2-enal; NADPH


substrate_name: Glutathione-4-oxonon-2-enal; NADPH

substrate: Glutathione-4-oxonon-2-enal; NADPH


organism: Anaerobiospirillum succiniciproducens

substrate_name: oxaloacetate

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

substrate: oxaloacetate


proof_lines: pH 7.5, 30°C, above, D263N and H225Q mutants


organism: Ricinus communis

substrate_name: phosphoenolpyruvate

proof_lines:
mutant enzyme S425A, at pH 8.0 and 25°C
mutant enzyme S425A, at pH 7.3 and 25°C

substrate: phosphoenolpyruvate


proof_lines:
mutant enzyme S451D, at pH 7.3 and 25°C
mutant enzyme S451D, at pH 8.0 and 25°C


proof_lines: mutant enzyme S425A/S451D, at pH 7.3 and 25°C

proof_lines: mutant enzyme S425A/S451D, at pH 8.0 and 25°C


proof_lines:
wild type enzyme, at pH 7.3 and 25°C
wild type enzyme, at pH 8.0 and 25°C


enzyme_display_name: chitinase

organism: Vibrio harveyi

proof_lines:
mutant W275G, 37°C
pH 5.0, 30°C, mutant enzyme W275G

enzyme: chitinase


substrate_name: colloidal chitin

proof_lines: pH 5.5, 37°C, colloidal chitin, wild-type enzyme

substrate: colloidal chitin


proof_lines:
mutant W397F, 37°C
pH 5.0, 30°C, mutant enzyme W397F


organism: Ascaris suum

substrate_name: GDP

substrate: GDP


substrate_name: GTP

substrate: GTP


substrate_name: 2'-deoxyguanosine 5'-diphosphate

substrate: 2'-deoxyguanosine 5'-diphosphate


substrate_name: 2'-deoxyguanosine 5'-triphosphate

substrate: 2'-deoxyguanosine 5'-triphosphate


organism: Vibrio parahaemolyticus


mutation_signature: C-terminal truncated G589 mutant enzyme VpChiAG589


proof_lines: pH 7, 37°C, C-terminal truncated G589 mutant enzyme VpChiAG589


enzyme_display_name: penicillin amidase

organism: Escherichia coli

substrate_name: Unknown

enzyme: penicillin amidase

substrate: Unknown


organism: Corynebacterium glutamicum

proof_lines:
wild-type, pH 7.5, 30Â°C
wild type enzyme, at pH 8.5 and 25°C


enzyme_display_name: 4-hydroxyphenylpyruvate dioxygenase

organism: Arabidopsis thaliana

verification_status: unverified

proof_lines: recombinant wild-type enzyme, pH and temperature not specified in the publication

enzyme: 4-hydroxyphenylpyruvate dioxygenase

status: unverified


proof_lines: recombinant mutant Q272E, pH and temperature not specified in the publication


enzyme_display_name: firefly luciferase

organism: Photinus pyralis

substrate_name: ATP

assay_conditions_summary: pH 8.5; 298.15 K; cofactor ATP

enzyme: firefly luciferase

substrate: ATP


substrate_name: D-luciferin

assay_conditions_summary: pH 7; 298.15 K; cofactor ATP

substrate: D-luciferin


proof_lines: pH 7.5, mutant enzyme A18E/N21W/R22N/N101D/K105D


organism: Schistosoma mansoni

substrate_name: GSSG

proof_lines: truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 150 mM potassium phosphate pH 7.0, at 37°C

proof_lines: truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 100 mM potassium phosphate pH 7.0, at 25°C

substrate: GSSG


substrate_name: 5,5'-dithiobis(2-nitrobenzoic acid)

proof_lines: full length enzyme, in 100 mM potassium phosphate pH 7.0, at 25°C

proof_lines: full length enzyme, in 150 mM potassium phosphate pH 7.0, at 37°C

substrate: 5,5'-dithiobis(2-nitrobenzoic acid)


substrate_name: methylenetetrahydrofolate

substrate: methylenetetrahydrofolate


organism: Homo sapiens

assay_conditions_summary: pH 7.4; 298.15 K; buffer 50 mM TES

proof_lines: mutant enzyme E30W, in 50 mM TES buffer, 50 mM, MgCl2, 13 mM formalin, 2 mM EDTA, 0.15 mM 2-mercaptoethanol, pH 7.4, at 25°C


proof_lines: wild type enzyme, in 50 mM TES buffer, 50 mM, MgCl2, 13 mM formalin, 2 mM EDTA, 0.15 mM 2-mercaptoethanol, pH 7.4, at 25°C

proof_lines: apparent value, wild type enzyme, pH not specified in the publication, at 37°C

proof_lines: wild type enzyme, in 50 mM Tris-HCl, pH 7.4, 1 mM EDTA, 0.2% 2-mercaptoethanol, at 22°C


enzyme_display_name: fructose-bisphosphate aldolase

organism: Anoxybacillus gonensis

substrate_name: D-Fructose 1,6-bisphosphate

enzyme: fructose-bisphosphate aldolase

substrate: D-Fructose 1,6-bisphosphate


organism: Cyanophora paradoxa


organism: Drosophila melanogaster


organism: Edwardsiella ictaluri


organism: Euglena gracilis


organism: Giardia lamblia


organism: Lactococcus lactis subsp. cremoris


organism: Lampetra japonica


organism: Leishmania mexicana


organism: Magnaporthe grisea 70-15


organism: Mus musculus


organism: Mycobacterium tuberculosis


organism: Oryctolagus cuniculus


organism: Pisum sativum


organism: Pyrococcus furiosus


organism: Rattus norvegicus

substrate_name: D-Fructose 1-phosphate

substrate: D-Fructose 1-phosphate


organism: Saccharomyces cerevisiae


organism: Arabidopsis thaliana


organism: Bacillus methanolicus


organism: Bacillus subtilis


organism: Entamoeba histolytica

substrate_name: D-Glyceraldehyde 3-phosphate; Glycerone phosphate


organism: Escherichia coli


organism: Halothece sp. PCC 7418


organism: Methanocaldococcus jannaschii


organism: Plasmodium falciparum


organism: Spinacia oleracea


substrate_name: nicotinamide ribonucleotide

substrate: nicotinamide ribonucleotide


organism: Francisella tularensis

substrate_name: ADP-ribose

assay_conditions_summary: pH 8.2; 310.15 K; cofactor ADP

substrate: ADP-ribose


organism: Giardia duodenalis

assay_conditions_summary: pH 7.5; 299.15 K; cofactor ATP

proof_lines: pH 7.5, 26°C, recombinant isozyme GlNMNAT-A


organism: Homo sapiens

substrate_name: NMNH

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

proof_lines:
pH 7.5, 37°C, isozyme NMNAT2
pH 7.5, 37°C, isozyme NMNAT3
pH 7.5, 37°C, isozyme NMNAT1

substrate: NMNH


assay_conditions_summary: pH 7.5; 315.15 K; cofactor ATP


organism: Leishmania braziliensis

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

proof_lines: pH 7.4, 37°C, recombinant enzyme, kinetics following Eadie-Hofstee method

proof_lines: pH 7.4, 37°C, recombinant enzyme, kinetics following Hanes-Woolf method

proof_lines: pH 7.4, 37°C, recombinant enzyme, kinetics following Lineweaver-Burk method


organism: Mus musculus

proof_lines: isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C


proof_lines: isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C


proof_lines: isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C


assay_conditions_summary: cofactor ATP

proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant C41A/C43A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant C43A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant C41A, pH not specified in the publication, temperature not specified in the publication


organism: Sulfolobus solfataricus

substrate_name: Dihydroxyacetone phosphate; D-Glyceraldehyde 3-phosphate


organism: Synechocystis sp.


organism: Thermococcus kodakaraensis


organism: Thermoproteus tenax


organism: Thermus aquaticus


organism: Triticum aestivum


organism: Trypanosoma brucei


organism: Zea mays


enzyme_display_name: thymidine kinase

organism: Dictyostelium discoideum

substrate_name: ATP; Thymidine

enzyme: thymidine kinase

substrate: ATP; Thymidine


organism: Epstein-Barr virus


organism: Human herpesvirus 1


substrate_name: ATP; 1-beta-D-Arabinofuranosylthymine

substrate: ATP; 1-beta-D-Arabinofuranosylthymine


organism: Rattus norvegicus


organism: Staphylococcus aureus


organism: Escherichia coli


organism: Homo sapiens

substrate_name: nicotinic acid mononucleotide

assay_conditions_summary: cofactor NAD+

substrate: nicotinic acid mononucleotide


proof_lines: product inhibition of NMNAT3, substrate NMN


organism: Saccharomyces cerevisiae

substrate_name: diphosphate

substrate: diphosphate


organism: Thermus thermophilus

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP


organism: Unknown


organism: Methanothermobacter thermautotrophicus

assay_conditions_summary: pH 7.5; 338.15 K; cofactor ATP


substrate_name: NMN

substrate: NMN


enzyme_display_name: aralkylamine N-acetyltransferase

organism: Ovis aries

substrate_name: tryptamine

proof_lines: pH 6.8, 30°C, mutant enzyme S205A, phosphorylated T31

proof_lines: pH 6.8, 30°C, mutant enzyme S205A, unphosphorylated

enzyme: aralkylamine N-acetyltransferase

substrate: tryptamine


proof_lines: pH 6.8, 30°C, mutant enzyme T31A, phosphorylated S205

proof_lines: pH 6.8, 30°C, mutant enzyme T31A, unphosphorylated


enzyme_display_name: corrinoid adenosyltransferase

substrate_name: cob(I)alamin

proof_lines: pH 8.0, 37°C, recombinant GST-tagged mutant R191W

enzyme: corrinoid adenosyltransferase

substrate: cob(I)alamin


proof_lines: pH 8.0, 37°C, recombinant wild-type GST-tagged enzyme


enzyme_display_name: cysteine synthase

substrate_name: O-acetyl-L-serine

enzyme: cysteine synthase

substrate: O-acetyl-L-serine


proof_lines:
wild-type, 37°C
wild-type


organism: Thermotoga maritima

substrate_name: ATP; Deoxythymidine

substrate: ATP; Deoxythymidine


organism: Vaccinia virus

substrate_name: Deoxythymidine; ATP

substrate: Deoxythymidine; ATP


enzyme_display_name: pyroglutamyl-peptidase II

substrate_name: Thyroliberin

enzyme: pyroglutamyl-peptidase II

substrate: Thyroliberin


enzyme_display_name: coagulation factor XIIa

proof_lines: recombinant His-tagged betaFXIIa, pH and temperature not specified in the publication

proof_lines: recombinant MBP-tagged betaFXIIa, pH and temperature not specified in the publication

proof_lines: alphaFXIIa, pH and temperature not specified in the publication

enzyme: coagulation factor XIIa


organism: Salmonella enterica subsp. enterica serovar Typhimurium

proof_lines: turnover for mutant K120Q is decreased 56fold compared to wild type, 25°C, 100 mM HEPES, pH 7.0

proof_lines: the turnover for H152A mutant is decreased 9fold compared to the wild type, 25°C, 100 mM MES, pH 6.5


organism: Mus musculus

assay_conditions_summary: pH 7.5; cofactor ATP

proof_lines: wild type enzyme, in 50 mM HEPES-NaOH, pH 7.5, temperature not specified in the publication


proof_lines: mutant enzyme C164S/C165S, in 50 mM HEPES-NaOH, pH 7.5, temperature not specified in the publication


substrate_name: UTP

proof_lines: recombinant wild-type enzyme, pH 8.0, 25°C

substrate: UTP


substrate_name: Unknown

substrate: Unknown


substrate_name: 4-nitrophenyl phosphate

assay_conditions_summary: pH 7; 295.15 K; cofactor ATP

substrate: 4-nitrophenyl phosphate


substrate_name: ADP

substrate: ADP


organism: Aeromonas caviae

proof_lines: pH 6.0, 50°C, recombinant His-tagged wild-type enzyme


proof_lines: pH 6.0, 50°C, recombinant His-tagged truncation mutant enzyme


organism: Manduca sexta


proof_lines:
37°C, pH 6, Chi386(MCBD)2
37°C, pH 6, Chi386
37°C, pH 6, Chi386(MCBD)


organism: Trichoderma viride


enzyme_display_name: scytalidoglutamic peptidase

organism: Scytalidium lignicola

proof_lines: pH and temperature not specified in the publication

enzyme: scytalidoglutamic peptidase


enzyme_display_name: pyrimidine-nucleoside phosphorylase

organism: Streptococcus pyogenes

substrate_name: uridine

proof_lines: mutant H169A, pH 7.5, temperature not specified in the publication

enzyme: pyrimidine-nucleoside phosphorylase

substrate: uridine


proof_lines: wild-type, pH 7.5, temperature not specified in the publication


source_db: skid

source_license: CC BY-NC-ND 4.0

source: skid


substrate_name: thymidine

substrate: thymidine


enzyme_display_name: polo kinase

organism: Danio rerio

substrate_name: ATP

proof_lines:
zPlk1 1-312 T196D
apparent Km, zPlk1 1-312 T196D

enzyme: polo kinase

substrate: ATP


proof_lines:
zPlk1 1-312 wild type
apparent Km, zPlk1 1-312 wild type


source_license: CC BY 4.0; CC BY-NC-ND 4.0

proof_lines:
time-dependent irreversible inhibition
Raw SKiD Env: Temp=-----, pH=-----

source_databases_merged: brenda skid


proof_lines:
time-dependent inhibition
Raw SKiD Env: Temp=-----, pH=-----


proof_lines: wild-type enzyme, varied substrate Cdc25c, constant substrate ATP

proof_lines: apparent Km, wild-type enzyme, varied substrate ATP, constant substrate Cdc25c


proof_lines: T210V mutant, varied substrate Cdc25c, constant substrate ATP

proof_lines: apparent Km, T210V mutant, varied substrate ATP, constant substrate Cdc25c


proof_lines: T210D mutant, varied substrate Cdc25c, constant substrate ATP

proof_lines: T210D mutant, varied substrate ATP, constant substrate Cdc25c


organism: Vibrio harveyi

substrate_name: chitohexaose

proof_lines: pH 5.5, 37°C, colloidal chitin, mutant enzym S33A

substrate: chitohexaose


proof_lines: pH 5.5, 37°C, colloidal chitin, mutant enzym Y245W


organism: Homo sapiens

proof_lines: mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C


proof_lines: wild type enzyme alphaFXIIa, at pH 7.4 and 37°C


enzyme_display_name: subtilisin

organism: Bacillus amyloliquefaciens

enzyme: subtilisin


proof_lines:
pH 7.4, 37°C, wild-type
wild-type


substrate_name: succinyl-Ala-Ala-Pro-Phe 4-nitroanilide

substrate: succinyl-Ala-Ala-Pro-Phe 4-nitroanilide


organism: Lederbergia lenta

substrate_name: succinyl-AAPR-p-nitroanilide

proof_lines:
pH 8.6, 25°C mutant S166C-S-d
pH 8.6, 25°C mutant S166C-S-j
pH 8.6, 25°C mutant S166C-S-g

substrate: succinyl-AAPR-p-nitroanilide


substrate_name: succinyl-AAPA-p-nitroanilide

proof_lines: pH 8.6, 25°C mutant S166C-S-e and S166S-f

substrate: succinyl-AAPA-p-nitroanilide


enzyme_display_name: limit dextrinase

organism: Hordeum vulgare

substrate_name: pullulan

proof_lines: fitting the uncompetitive substrate inhibition Michaelis-Menten equation, pH 5.5, 40°C

proof_lines: classical Michaelis-Menten kinetics, pH 5.5, 40°C

enzyme: limit dextrinase

substrate: pullulan


proof_lines: wild-type, 37°C, pH not specified in the publication


substrate_name: amylopectin

proof_lines: mutant M440G, 37°C, pH not specified in the publication

substrate: amylopectin


organism: Hordeum vulgare subsp. vulgare

substrate_name: beta-limit dextrin

substrate: beta-limit dextrin


substrate_name: L-Tyr-L-Val-L-Ala-L-Asp

proof_lines:
wild-type, pH 9.0, 24°C
pH 8.6, 25°C wild-type
pH 7.5, 25°C, wild-type

substrate: L-Tyr-L-Val-L-Ala-L-Asp


substrate_name: L-Ala-L-Ala-L-Val-L-Ala

substrate: L-Ala-L-Ala-L-Val-L-Ala


enzyme_display_name: N-acyl-D-amino-acid deacylase

organism: Achromobacter xylosoxidans

substrate_name: N-Acetyl-D-Trp

proof_lines: mutant enzyme L298A, in 100 mM HEPES (pH 7.0), at 30°C

enzyme: N-acyl-D-amino-acid deacylase

substrate: N-Acetyl-D-Trp


substrate_name: N-Acetyl-D-Ala

proof_lines: wild type enzyme, in 100 mM HEPES (pH 7.0), at 30°C

substrate: N-Acetyl-D-Ala


proof_lines: mutant enzyme F191W, in 100 mM HEPES (pH 7.0), at 30°C


organism: Alcaligenes faecalis

substrate_name: N-Acetyl-D-Met

substrate: N-Acetyl-D-Met


organism: Microbacterium natoriense

substrate_name: N-acetyl-D-phenylalanine

substrate: N-acetyl-D-phenylalanine


organism: Variovorax paradoxus

substrate_name: N-acetyl-D-methionine

proof_lines: recombinant mutant M254L, pH and temperature not specified in the publication

substrate: N-acetyl-D-methionine


proof_lines: recombinant mutant M39L, pH and temperature not specified in the publication


proof_lines: recombinant mutant M56L, pH and temperature not specified in the publication


proof_lines: recombinant mutant M221L, pH and temperature not specified in the publication


proof_lines: recombinant mutant M352L, pH and temperature not specified in the publication


proof_lines: recombinant wild-type enzyme, pH and temperature not specified in the publication


substrate_name: N-acetyl-D-leucine

substrate: N-acetyl-D-leucine


enzyme_display_name: camphor 5-monooxygenase

organism: Pseudomonas putida

substrate_name: (+)-Camphor

enzyme: camphor 5-monooxygenase

substrate: (+)-Camphor


substrate_name: NADH

assay_conditions_summary: pH 7.4; 295.15 K; cofactor NADH

proof_lines: pH 7.4, 22°C, recombinant mutant H352A enzyme with mutant S42A putidaredoxin

substrate: NADH


proof_lines: pH 7.4, 22°C, recombinant mutant D125A enzyme with mutant Y33A putidaredoxin


proof_lines: mutant Y179H, pH 7.4, temperature not specified in the publication


proof_lines: mutant G60S/Y75H, pH 7.4, temperature not specified in the publication


proof_lines: mutant E156G/V247F/V253G/F256S, pH 7.4, temperature not specified in the publication


proof_lines: mutant G93C/K314R/L319M, pH 7.4, temperature not specified in the publication


proof_lines: mutant D97F/P122L/Q183L/L244Q, pH 7.4, temperature not specified in the publication


proof_lines: mutant G120A/Y179H/G248S/D297H, pH 7.4, temperature not specified in the publication


proof_lines: mutant T56A/N116H/D297N, pH 7.4, temperature not specified in the publication


substrate_name: reduced putidaredoxin

assay_conditions_summary: pH 7.4; 303.15 K; cofactor NADH

proof_lines: putidaredoxin reductase-camphor monooxygenase fusion enzyme, pH 7.4, 30°C

proof_lines: pH and temperature not specified in the publication, with P450tcu putidaredoxin

proof_lines: pH and temperature not specified in the publication, with P450 putidaredoxin

substrate: reduced putidaredoxin


proof_lines: pH 7.4, 22°C, recombinant mutant D125A enzyme with wild-type putidaredoxin

proof_lines: pH 7.4, 22°C, recombinant wild-type enzyme with mutant S44Aputidaredoxin


enzyme_display_name: tyramine N-feruloyltransferase

organism: Capsicum annuum

substrate_name: tyramine

enzyme: tyramine N-feruloyltransferase

substrate: tyramine


assay_conditions_summary: pH 8.5; 303.15 K; cofactor CoA


organism: Lavandula angustifolia

assay_conditions_summary: pH 7.5; 303.15 K; cofactor CoA

proof_lines: with tyramine, pH 7.5, 30°C, recombinant AAT1

proof_lines: with phenethylamine, pH 7.5, 30°C, recombinant AAT1

proof_lines: with caffeoyl-CoA, pH 7.5, 30°C, recombinant AAT1


enzyme_display_name: N-acetylgalactosamine kinase

substrate_name: N-acetyl-alpha-D-galactosamine

proof_lines:
pH 8.0, 37°C
Raw SKiD Env: Temp=37°C, pH=7.5
Raw SKiD Env: Temp=37°C, pH=8

enzyme: N-acetylgalactosamine kinase

substrate: N-acetyl-alpha-D-galactosamine


proof_lines: mutant F444C, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine

proof_lines: mutant F444C, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine


proof_lines: mutant F444W, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine


substrate_name: N-acetyl-alpha-D-mannosamine

proof_lines: mutant F444R, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine

proof_lines: mutant F444R, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine

substrate: N-acetyl-alpha-D-mannosamine


proof_lines: wild-type, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine

proof_lines: wild-type, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine


proof_lines: mutant F444H, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine

proof_lines: mutant F444H, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine


proof_lines: mutant F444K, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine

proof_lines: mutant F444K, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine


proof_lines: mutant F444S, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine

proof_lines: mutant F444S, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine


substrate_name: N-acetyl-alpha-D-glucosamine

proof_lines: mutant F444A, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine

proof_lines: mutant F444A, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine

substrate: N-acetyl-alpha-D-glucosamine


organism: uncultured bacterium

proof_lines: pH and temperature not specified in the publication


organism: Elizabethkingia meningoseptica

substrate_name: 4-nitrophenyl alpha-L-fucoside

substrate: 4-nitrophenyl alpha-L-fucoside


substrate_name: Lewis X trisaccharide

substrate: Lewis X trisaccharide


proof_lines: mutant enzyme 249DELTA265, at pH 4.6 and 37°C


enzyme_display_name: penicillin amidase

organism: Achromobacter sp.

substrate_name: methyl D-(-)-4-hydroxy-phenyl glycinate

enzyme: penicillin amidase

substrate: methyl D-(-)-4-hydroxy-phenyl glycinate


organism: Escherichia coli

substrate_name: D-phenylglycine amide

proof_lines:
wild-type enzyme
pH 7.0, 25°C, wild-type enzyme
wild-type, pH 7.0, 30°C

substrate: D-phenylglycine amide


proof_lines:
mutant alphaR145G
mutant alphaR145S
mutant alphaR145L


organism: Mesocricetus auratus

proof_lines: pH 7.4, 25°C, with substrate S-camphorquinone


organism: Rattus norvegicus


enzyme_display_name: alanine racemase

organism: Bacillus subtilis

substrate_name: L-alanine

enzyme: alanine racemase

substrate: L-alanine


organism: Geobacillus stearothermophilus

substrate_name: D-alanine

proof_lines: pH 8.0, 30°C, recombinant wild-type enzyme

substrate: D-alanine


substrate_name: D-Lysine

proof_lines: pH 8.0, 30°C, recombinant mutant I222T/Y354W

substrate: D-Lysine


organism: Helicobacter pylori

proof_lines:
Vmax 909 micromol/min/mg
Vmax 37.9 micromol/min/mg


organism: Streptomyces lavendulae


proof_lines: +/-0.250, Alr D164K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.033, Alr D164K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.070, Alr D164K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


proof_lines: +/-0.200, Alr D164A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-2.600, Alr D164A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.114, Alr D164A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


proof_lines: +/-0.333, Alr E221K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-7.550, Alr E221K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.055, Alr E221K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


proof_lines: +/-0.100, Alr E165K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.850, Alr E165K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.079, Alr E165K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


proof_lines: +/-0.417, Alr E165A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-2.667, Alr E165A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.085, Alr E165A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


proof_lines: +/-0.483, Alr wildtype, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-3.217, Alr wildtype, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.008, Alr wildtype, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


organism: Caldanaerobacter subterraneus subsp. tengcongensis


proof_lines: pH 10.0, 65°C, recombinant wild-type enzyme


proof_lines: +/-0.333, Alr P219A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-6.067, Alr P219A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.034, Alr P219A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


proof_lines: +/-0.750, Alr E221P, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-7.633, Alr E221P, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.084, Alr E221P, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


proof_lines: +/-0.650, Alr E221A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-2.333, Alr E221A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.080, Alr E221A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


proof_lines: pH 10.0, 65°C, recombinant mutant S173D/Q360Y


organism: Clostridioides difficile

proof_lines: pH 8.5, temperature not specified in the publication, recombinant wild-type enzyme

proof_lines: pH 7.5, 37°C, recombinant wild-type enzyme


proof_lines: pH 8.5, temperature not specified in the publication, recombinant mutant K271T


organism: Candidatus Pelagibacter communis

proof_lines: recombinant MBP-tagged enzyme, pH 8.0, 37°C


organism: Mycobacterium tuberculosis


organism: Synechocystis sp. PCC 6803


organism: Thermotoga maritima

assay_conditions_summary: pH 8; 310.15 K; buffer recombinant His-tagged enzyme

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C, Tris buffer

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C, bicine buffer

proof_lines: recombinant His-tagged enzyme, pH 8.0, 70°C, bicine buffer


organism: Wolbachia pipientis

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C


organism: Clostridium perfringens


enzyme_display_name: hepsin

organism: Homo sapiens

substrate_name: Unknown

assay_conditions_summary: pH 8

proof_lines: at pH 8.0, temperature not specified in the publication

proof_lines: pH not specified in the publication, temperature not specified in the publication

enzyme: hepsin

substrate: Unknown


substrate_name: D-Ala

substrate: D-Ala


enzyme_display_name: HIV-2 retropepsin

organism: Human immunodeficiency virus 2

enzyme: HIV-2 retropepsin


enzyme_display_name: orotidine-5'-phosphate decarboxylase

organism: Saccharomyces cerevisiae

substrate_name: orotidine 5'-phosphate

proof_lines:
Q215A mutant
mutant Q215A, pH 7.1, 25°C

enzyme: orotidine-5'-phosphate decarboxylase

substrate: orotidine 5'-phosphate


proof_lines: wild-type, pH 7.1, 25°C, presence of 0.1 M NaCl


enzyme_display_name: phosphoenolpyruvate carboxylase

organism: Echinochloa esculenta

proof_lines:
wild-type, pH 7.6, 25°C
wild-type, Hill value 1.1, pH 7.6, 25°C

enzyme: phosphoenolpyruvate carboxylase


proof_lines:
hybrid mutant V674-880, pH 7.6, 25°C
hybrid mutant V528-880, pH 7.6, 25°C
hybrid mutant V528-673, pH 7.6, 25°C


organism: Oryza sativa Japonica Group

substrate_name: phosphoenolpyruvate

proof_lines: mutant S12D, presence of glucose 6-phosphate, S0.5 value, Hill coeffcient 1.64, pH 7.3, 30°C

proof_lines: mutant S12D, S0.5 value, Hill coeffcient 1.10, pH 7.3, 30°C

substrate: phosphoenolpyruvate


proof_lines: wild-type, S0.5 value, Hill coeffcient 2.06, pH 7.3, 30°C

proof_lines: wild-type, presence of glucose 6-phosphate, S0.5 value, Hill coeffcient 1.84, pH 7.3, 30°C


enzyme_display_name: phosphoenolpyruvate carboxykinase (GTP)

organism: Sus scrofa

proof_lines: mutant M139L, substrate oxaloacetate, pH 7.4, temperature not specified in the publication

proof_lines: mutant M139L, substrate phosphoenolpyruvate, pH 7.4, temperature not specified in the publication

proof_lines: mutant M139L, substrate GDP, pH 7.4, temperature not specified in the publication

enzyme: phosphoenolpyruvate carboxykinase (GTP)


proof_lines: wild-type, substrate oxaloacetate, pH 7.4, temperature not specified in the publication

proof_lines: wild-type, substrate GDP, pH 7.4, temperature not specified in the publication

proof_lines: wild-type, substrate phosphoenolpyruvate, pH 7.4, temperature not specified in the publication


enzyme_display_name: (S)-hydroxynitrile lyase

organism: Manihot esculenta

substrate_name: acetone cyanohydrin

enzyme: (S)-hydroxynitrile lyase

substrate: acetone cyanohydrin


proof_lines:
wild-type enzyme
pH 5.4, 25°C, wild-type enzyme


enzyme_display_name: 2-dehydro-3-deoxy-L-fuconate aldolase

organism: Veillonella ratti

enzyme: 2-dehydro-3-deoxy-L-fuconate aldolase


enzyme_display_name: tryptophan synthase

substrate_name: indole

enzyme: tryptophan synthase

substrate: indole


substrate_name: L-serine

substrate: L-serine


organism: Salmonella enterica subsp. enterica serovar Typhimurium

proof_lines: overall-reaction, pH 7.8, 25°C, wild-type enzyme

proof_lines: beta-reaction, pH 7.8, 25°C, wild-type enzyme

proof_lines: alpha-reaction, wild-type enzyme, pH 7.8, 25°C


proof_lines: beta-reaction, pH 7.8, 25°C, mutant T183V enzyme

proof_lines: overall-reaction, pH 7.8, 25°C, mutant T183V enzyme

proof_lines: alpha-reaction, pH 7.8, 25°C, mutant T183V enzyme


substrate_name: L-threonine

proof_lines: mutant L166V, pH not specified in the publication, temperature not specified in the publication

substrate: L-threonine


proof_lines: mutant L166A, pH not specified in the publication, temperature not specified in the publication


enzyme_display_name: 4-hydroxyphenylpyruvate dioxygenase

organism: Echinochloa crus-galli

enzyme: 4-hydroxyphenylpyruvate dioxygenase


organism: Avena sativa


organism: Arabidopsis thaliana

proof_lines: activity is measured polarographically, assays include 0.5 microM of enzyme, 500 microM betaME, Fe2+ stoichiometric with enzyme, and 400 microM 4-hydroxyophenylpyruvate in 20 mM HEPES at pH 7.0 at 4°C


proof_lines:
enzyme form 1
enzyme form 3
enzyme form 2


organism: Streptomyces avermitilis

proof_lines: Solvent is H2O. Steady-state analyses of 4-hydroxyphenylpyruvate dioxygenase using proteo-HPP, 2,3,5,6-tetradeutero-HPP, and proteo-HPP in deuterium oxide are carried out in 1 ml assays on a Hasetech oxygen electrode. The assay mixture incl

proof_lines: Solvent is D2O. Steady-state analyses of 4-hydroxyphenylpyruvate dioxygenase using proteo-HPP, 2,3,5,6-tetradeutero-HPP, and proteo-HPP in deuterium oxide are carried out in 1 ml assays on a Hasetech oxygen electrode. The assay mixture incl


proof_lines: pH and temperature not specified in the publication