organism: Trypanosoma brucei

assay_conditions_summary: pH 7.1; 303.15 K; cofactor ATP

proof_lines: pH 7.1, 30°C, protein kinase A treated enzyme


substrate_name: ATP

source_db: brenda

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

protein_accession_database: UniProt

source_license: CC BY 4.0

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

substrate: ATP

source: brenda


enzyme_display_name: acetate kinase

organism: Enterococcus faecalis

substrate_name: ATP; Acetate

km_display: Not reported

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

assay_conditions_summary: cofactor ATP

enzyme: acetate kinase

substrate: ATP; Acetate


organism: Escherichia coli

source_db: oed

assay_conditions_summary: pH 7.5; 283.15 K; cofactor ATP

source: oed


substrate_name: acetyl phosphate

assay_conditions_summary: pH 7.5; 288.15 K; cofactor ATP

proof_lines:
pH 7.5, 15°C, recombinant enzyme
pH 7.5, 10°C, recombinant enzyme
pH 7.5, 25°C, recombinant enzyme

substrate: acetyl phosphate


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution

assay_conditions_summary: pH 6.5; 303.15 K; cofactor ATP


proof_lines: pH 6.5, 30°C, recombinant mutant N213T/G332D/E336L/T385N


proof_lines: pH 6.5, 30°C, recombinant wild-type enzyme


organism: Homo sapiens

substrate_name: beta-D-fructose 6-phosphate

proof_lines: pH 7.5, 30°C, recombinant HBP1 mutant S460D/T470D

substrate: beta-D-fructose 6-phosphate


proof_lines: pH 7.5, 30°C, recombinant HBP1 mutant S477D

proof_lines: pH 7.5, 30°C, recombinant non-phosphorylated, HBP1 mutant S477D


proof_lines: pH 7.5, 30°C, recombinant HBP1 mutant H253A/S460D

proof_lines: pH 7.5, 30°C, recombinant non-phosphorylated, HBP1 mutant H253A/S460D


proof_lines: pH 7.5, 30°C, recombinant non-phosphorylated, HBP1 mutants T470D and S460D/T470D


proof_lines: pH 7.5, 30°C, recombinant His-tagged wild-type HBP1

proof_lines: pH 7.5, 30°C, recombinant His-tagged wild-type HBP2

proof_lines: pH 7.5, 30°C, recombinant wild-type HBP1


proof_lines: pH 7.5, 30°C, recombinant HBP1 mutant T470

proof_lines: pH 7.5, 30°C, recombinant non-phosphorylated, His-tagged chimeric mutant enzyme

proof_lines: pH 7.5, 30°C, recombinant phosphorylated, His-tagged chimeric mutant enzyme


substrate_name: D-Fructose 6-phosphate

assay_conditions_summary: pH 8.5; 298.15 K; cofactor ATP

substrate: D-Fructose 6-phosphate


substrate_name: D-Fructose 6-phosphate; ATP

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

proof_lines: inhibition of recombinant PFKFB3 protein, no inhibition of purified PFK-1 activity

source_databases_merged: sabio_rk brenda skid

substrate: D-Fructose 6-phosphate; ATP


substrate_name: ATP; beta-D-Fructose 6-phosphate

substrate: ATP; beta-D-Fructose 6-phosphate


organism: Rattus norvegicus


enzyme_display_name: creatine kinase

substrate_name: Phosphocreatine

smiles: CN(CC(=O)O)C(=N)NP(=O)(O)O

enzyme: creatine kinase

substrate: Phosphocreatine


substrate_name: Creatine

smiles: CN(CC(=O)O)C(=N)N

substrate: Creatine


substrate_name: ADP

assay_conditions_summary: pH 7; 303.15 K; cofactor ADP

substrate: ADP


assay_conditions_summary: pH 9; 303.15 K; cofactor ATP


assay_conditions_summary: pH 8; 298.15 K; cofactor ATP

proof_lines: recombinant mutant enzyme H66P/D326A, at 25Â°C, pH not specified in the publication

proof_lines: mutant enzyme H66P/D326A, at pH 8.0 and 25°C


proof_lines: recombinant wild type enzyme, at 25Â°C, pH not specified in the publication


proof_lines: recombinant mutant enzyme H66P, at 25Â°C, pH not specified in the publication


proof_lines: recombinant mutant enzyme H66R, at 25Â°C, pH not specified in the publication


organism: Methanosarcina thermophila

assay_conditions_summary: pH 7; 310.15 K; cofactor ATP


substrate_name: ITP

substrate: ITP


substrate_name: TTP

substrate: TTP


substrate_name: UTP

substrate: UTP


substrate_name: GTP

substrate: GTP


substrate_name: CTP

substrate: CTP


proof_lines:
37°C, pH 7.0-7.5, wild-type enzyme
wild-type, pH 7.4
wild-type


assay_conditions_summary: pH 7; cofactor ATP

proof_lines:
mutant R241L, pH 7.0
mutant R241L, pH 7.4


assay_conditions_summary: pH 7.4; cofactor ATP

proof_lines: mutant R241A, pH 7.0, presence of 200 mM guanidine/HCl


proof_lines:
mutant R91L, pH 7.4
mutant R91L, pH 7.0


proof_lines:
mutant R241K, pH 7.0
mutant R241K, pH 7.4


proof_lines: recombinant mutant enzyme D326E, at 25Â°C, pH not specified in the publication


proof_lines: recombinant mutant enzyme D326A, at 25Â°C, pH not specified in the publication


substrate_name: ADP; Phosphocreatine

substrate: ADP; Phosphocreatine


substrate_name: Phosphocreatine; dCDP

substrate: Phosphocreatine; dCDP


substrate_name: ATP

source_db: skid

source_license: CC BY-NC-ND 4.0

proof_lines:
Raw SKiD Env: Temp=25°C, pH=8
Raw SKiD Env: Temp=25Â°C, pH=-----

substrate: ATP

source: skid


organism: Oryctolagus cuniculus


substrate_name: ATP; Creatine

substrate: ATP; Creatine


assay_conditions_summary: 298.15 K; cofactor ATP


assay_conditions_summary: pH 9; 298.15 K; cofactor ATP

proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=25°C, pH=-----
Raw SKiD Env: Temp=30°C, pH=9


enzyme_display_name: adenylate kinase

organism: Arabidopsis thaliana

substrate_name: ATP; AMP

enzyme: adenylate kinase

substrate: ATP; AMP


organism: Bacillus subtilis

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

assay_conditions_summary: pH 7.2; 328.15 K; cofactor ATP

proof_lines:
Raw SKiD Env: Temp=55°C, pH=7.2
Raw SKiD Env: Temp=45°C, pH=7.2
Raw SKiD Env: Temp=35°C, pH=7.2


substrate_name: AMP

proof_lines:
wild-type, pH 7.2, 55°C
wild-type, pH 7.2, 45°C
wild-type, pH 7.2, 27°C

substrate: AMP


assay_conditions_summary: pH 5.5; 310.15 K; cofactor ATP

proof_lines: 37°C, pH is not specified in the publication

proof_lines: pH 5.5, temperature not specified in the publication

proof_lines: pH and temperature not specified in the publication


assay_conditions_summary: pH 7; 355.15 K; cofactor ATP


substrate_name: acetate

smiles: CC(=O)O

assay_conditions_summary: pH 5.5; 355.15 K; cofactor ATP

substrate: acetate


substrate_name: acetyl phosphate

smiles: CC(=O)OP(=O)(O)O

substrate: acetyl phosphate


proof_lines: mutant enzyme G331Q/I332M, at pH 7.0 and 37Â°C

proof_lines: mutant enzyme G331Q/I332M, at pH 5.5 and 45Â°C


organism: Methylotuvimicrobium alcaliphilum

assay_conditions_summary: pH 8; 303.15 K; cofactor ATP


organism: Porphyromonas gingivalis

assay_conditions_summary: pH 8; cofactor ATP


organism: Salmonella enterica subsp. enterica serovar Typhimurium

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

proof_lines:
pH 7.5, 37°C
co-substrate: acetate, pH 7.5, 37°C
co-substrate: propionate, pH 7.5, 37°C


organism: Shewanella sp.

assay_conditions_summary: pH 7.5; 283.15 K; cofactor ATP

proof_lines:
pH 7.5, 10°C, recombinant enzyme
pH 7.5, 15°C, recombinant enzyme
pH 7.5, 25°C, recombinant enzyme


organism: Cryptosporidium muris

substrate_name: glycocyamine

smiles: N=C(N)NCC(=O)O

assay_conditions_summary: pH 8.5; 298.15 K; cofactor ATP

substrate: glycocyamine


organism: Gallus gallus

assay_conditions_summary: cofactor ATP


source_license: CC BY 4.0; CC BY-NC-ND 4.0

assay_conditions_summary: pH 9; 303.15 K; cofactor ATP

proof_lines:
ATP in form of MgATP2-
Raw SKiD Env: Temp=30°C, pH=9
Raw SKiD Env: Temp=-----, pH=-----

source_databases_merged: brenda skid


substrate_name: N-ethylglycocyamine

proof_lines: pH 9.0, 30°C, recombinant wild-type enzyme, with substrate N-ethylglycocyamine

proof_lines: pH 9.0, 30°C, recombinant wild-type enzyme, with substrate creatine

substrate: N-ethylglycocyamine


substrate_name: Phosphocreatine

substrate: Phosphocreatine


proof_lines:
mutant Q199R, pH 7.2, 60°C
mutant Q199R, pH 7.2, 45°C
mutant Q199R, pH 7.2, 55°C


organism: Bordetella pertussis

substrate_name: ADP

substrate: ADP


organism: Bos taurus


assay_conditions_summary: cofactor ADP


organism: Clonorchis sinensis

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ADP

proof_lines: in 100 mM Tris–HCl (pH 7.5), 20 mM glucose, 5 mM MgCl2, 10 mM KCl, 2 mM dithiothreitol, at 37°C


organism: Cyprinus carpio


organism: Escherichia coli

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

proof_lines: closed conformation, pH and temperature not specified in the publication

proof_lines: open conformation, pH and temperature not specified in the publication


organism: Geobacillus stearothermophilus


organism: Homo sapiens

proof_lines: nucleotide-binding domain 1 of wild type

proof_lines: nucleotide-binding domain 2 of wild type


mutation_type: deletion

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP

proof_lines:
mutant D140del, 37°C, pH 8.0
mutant D140del, 37°C


organism: Plasmodium falciparum

assay_conditions_summary: pH 6; cofactor ATP


assay_conditions_summary: pH 7.6; 298.15 K; buffer TEA-HCl; cofactor ATP

proof_lines: recombinant adenylate kinase 2, in 110 mM TEA-HCl, pH 7.6, at 25°C


organism: Saccharomyces cerevisiae


organism: Streptococcus pneumoniae

substrate_name: Unknown

ki_unit: mM

substrate: Unknown


organism: Strongylocentrotus purpuratus

assay_conditions_summary: pH 7.6; 289.15 K; cofactor ADP


organism: Sulfolobus acidocaldarius

assay_conditions_summary: 343.15 K; cofactor ATP

proof_lines: 70°C, pH is not specified in the publication

proof_lines: 90°C, pH is not specified in the publication


organism: Thermotoga neapolitana

assay_conditions_summary: pH 7.4; 303.15 K; cofactor ATP


substrate_name: ATP; Nicotinamide nucleotide

assay_conditions_summary: cofactor NAD+

substrate: ATP; Nicotinamide nucleotide


substrate_name: nicotinamide ribonucleotide

substrate: nicotinamide ribonucleotide


proof_lines:
mutant G64R, 37°C, pH 8.0
mutant G64R, 37°C


proof_lines:
mutant Y164C, 37°C, pH 8.0
mutant Y164C, 37°C


proof_lines:
mutant G40R, 37°C, pH 8.0
mutant G40R, 37°C


assay_conditions_summary: 310.15 K; cofactor ATP


proof_lines:
wild-type, 37°C, pH 8.0
wild-type, 37°C


proof_lines: Km above 2.0 mM, adenylate kinase 5 domain AK5p1, with ATP as phosphate donor


proof_lines: recombinant adenylate kinase 4, using GTP as cosubstrate

proof_lines: recombinant adenylate kinase 4, using ATP as cosubstrate


assay_conditions_summary: pH 8; 310.15 K; cofactor ADP

proof_lines:
Raw SKiD Env: Temp=37°C, pH=-----
Raw SKiD Env: Temp=37°C, pH=8


assay_conditions_summary: pH 7.5; cofactor ATP

proof_lines: mutant enzyme CINAP H79G, in 100 mM Tris-HCl, pH 7.5, 60 mM KCl, temperature not specified in the publication


substrate_name: Nicotinamide nucleotide

substrate: Nicotinamide nucleotide


substrate_name: Tiazofurin monophosphate

substrate: Tiazofurin monophosphate


substrate_name: ITP

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

substrate: ITP


substrate_name: nicotinic acid mononucleotide

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NAD+

proof_lines: product inhibition of NMNAT3, substrate NMN

substrate: nicotinic acid mononucleotide


substrate_name: ATP

substrate: ATP


substrate_name: Nicotinate D-ribonucleotide

substrate: Nicotinate D-ribonucleotide


assay_conditions_summary: pH 7.5; 315.15 K; cofactor ATP


organism: Leishmania braziliensis


assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP


organism: Methanothermobacter thermautotrophicus

assay_conditions_summary: pH 7.5; 338.15 K; cofactor ATP


assay_conditions_summary: pH 7.5; 338.15 K; cofactor NAD+


organism: Giardia duodenalis

assay_conditions_summary: pH 7.5; 299.15 K; cofactor ATP

proof_lines: pH 7.5, 26°C, recombinant isozyme GlNMNAT-A


organism: Haemophilus influenzae

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP


substrate_name: Nicotinamide nucleotide; ATP

substrate: Nicotinamide nucleotide; ATP


substrate_name: GTP

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NAD+

substrate: GTP


substrate_name: ATP; Tiazofurin monophosphate

substrate: ATP; Tiazofurin monophosphate


proof_lines: wild type enzyme CINAP, in 100 mM Tris-HCl, pH 7.5, 60 mM KCl, temperature not specified in the publication


substrate_name: AMP

assay_conditions_summary: cofactor ATP

substrate: AMP


organism: Jeotgalibacillus marinus

assay_conditions_summary: pH 7.2; 303.15 K; buffer the same phosphate; cofactor ATP

proof_lines: at 30°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 65 mM KCl

proof_lines: at 22°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 65 mM KCl

proof_lines: at 40°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 65 mM KCl


organism: Leishmania donovani

assay_conditions_summary: pH 8; cofactor ATP


organism: Megalodesulfovibrio gigas

assay_conditions_summary: pH 7.6; buffer the same Tris-HCl; cofactor ATP

proof_lines: Co2+-containing recombinant adenylate kinase, in 50 mM Tris-HCl, pH 7.6, 100 mM KCl, 0.25 mM MgCl2

proof_lines: Zn2+-containing recombinant adenylate kinase, in 50 mM Tris-HCl, pH 7.6, 100 mM KCl, 0.25 mM MgCl2

proof_lines: Fe2+-containing recombinant adenylate kinase, in 50 mM Tris-HCl, pH 7.6, 100 mM KCl, 0.25 mM MgCl2


organism: Methanococcus maripaludis

assay_conditions_summary: pH 7.2; 313.15 K; buffer the same phosphate; cofactor ATP

proof_lines: at 40°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2

proof_lines: at 30°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2

proof_lines: at 50°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2


organism: Mus musculus


organism: Oryctolagus cuniculus

substrate_name: ATP; AMP

substrate: ATP; AMP


organism: Penicillium chrysogenum


assay_conditions_summary: cofactor NAD+


organism: Plasmodium falciparum


organism: Salmonella enterica


organism: Salmonella typhimurium


enzyme_display_name: acetylcholinesterase

organism: Bungarus fasciatus

substrate_name: H2O; Acetylcholine

enzyme: acetylcholinesterase

substrate: H2O; Acetylcholine


organism: Dictyocaulus viviparus

substrate_name: Acetylthiocholine; H2O

substrate: Acetylthiocholine; H2O


enzyme_display_name: alkaline phosphatase

organism: Escherichia coli

substrate_name: 4-nitrophenyl phosphate

proof_lines:
pH 8.0, 25°C, mutant R166S
mutant enzyme R166S, at pH 8.0 and 25°C

enzyme: alkaline phosphatase

substrate: 4-nitrophenyl phosphate


proof_lines: mutant enzyme R166S/D153A/E322Y, at pH 8.0 and 25°C


proof_lines: mutant enzyme R166S/D153A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/D153A/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/R166S/D153A/E322Y/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme R166S/E322Y, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/R166S/E322Y, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/E322Y/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D153A/E322Y/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme R166S/D153A/E322Y/K328A, at pH 8.0 and 25°C


organism: Electrophorus electricus

substrate_name: H2O; Acetylthiocholine

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: H2O; Acetylthiocholine


organism: Homo sapiens

proof_lines: recombinant enzyme, pH and temperature not specified in the publication


substrate_name: Acetylthiocholine

smiles: CC(=O)SCC[N+](C)(C)C

substrate: Acetylthiocholine


substrate_name: acetylthiocholine

substrate: acetylthiocholine


organism: Stomoxys calcitrans

assay_conditions_summary: pH 7.5; 298.15 K; buffer potassium phosphate

proof_lines: in potassium phosphate buffer (100 mM, pH 7.5), at 25°C


proof_lines: mutant enzyme E322Y/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/E322Y, at pH 8.0 and 25°C


proof_lines: mutant enzyme D153A/E322Y, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/D153A/E322Y, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/R166S, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/R166S/D153A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D153A/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/R166S/D153A/E322Y, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/D153A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/R166S/D153A/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme R166S/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme R166S/D153A/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/D153A/E322Y/K328A, at pH 8.0 and 25°C


organism: Antarctic bacterium TAB5


substrate_name: 4-Nitrophenyl phosphate

substrate: 4-Nitrophenyl phosphate


proof_lines: pH 8.0, 25°C, recombinant wild-type enzyme


substrate_name: H2O; 4-Nitrophenyl phosphate

assay_conditions_summary: buffer contexts

substrate: H2O; 4-Nitrophenyl phosphate


proof_lines: mutant enzyme D101A/R166S/E322Y/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme R166S/E322Y/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/R166S/K328A, at pH 8.0 and 25°C


substrate_name: methyl phosphate

substrate: methyl phosphate


organism: Gallus gallus


organism: Geobacillus thermodenitrificans


organism: Halobacterium salinarum

proof_lines:
pH 10.5, 20°C, 4 M NaCl
Raw SKiD Env: Temp=20°C, pH=10.5


organism: Helicoverpa armigera


substrate_name: H2O; Phenolic phosphate

substrate: H2O; Phenolic phosphate


organism: Bacillus licheniformis


organism: Bos taurus

substrate_name: 3-(4-methoxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]-decan]-4-yl)phenyl phosphate

proof_lines: direct physical adsorption, immobilized ALP


substrate_name: Phosphoenolpyruvate; H2O

substrate: Phosphoenolpyruvate; H2O


organism: Burkholderia cenocepacia

substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


organism: Cobetia marina


proof_lines:
25°C, pH 18.0, Tris-HCl buffer
25°C, pH 10.3, diethanolamine buffer
25°C, pH 18.0, diethanolamine buffer


substrate_name: 4-Nitrophenyl phosphate; H2O

proof_lines:
single-chain Fv antibody fusion protein
Raw SKiD Env: Temp=25°C, pH=8
Raw SKiD Env: Temp=-----, pH=-----

substrate: 4-Nitrophenyl phosphate; H2O


organism: Mus musculus

substrate_name: Unknown

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: Unknown


organism: Rattus norvegicus


organism: Saccharococcus caldoxylosilyticus

proof_lines: pH 9.5, 50°C, Vmax: 0.049 micromol/min/mg


organism: Shewanella sp. T3-3


organism: Sphingobium sp. TCM1


organism: Thermus sp.


organism: Thermus sp. NTU-237


organism: Escherichia coli

substrate_name: GMP

substrate: GMP


substrate_name: AMP

substrate: AMP


substrate_name: dGMP

substrate: dGMP


substrate_name: dTMP

substrate: dTMP


substrate_name: dUMP

substrate: dUMP


substrate_name: UMP

substrate: UMP


substrate_name: dAMP

substrate: dAMP


substrate_name: dCMP

substrate: dCMP


substrate_name: dIMP

substrate: dIMP


substrate_name: 4-Nitrophenyl phosphate

substrate: 4-Nitrophenyl phosphate


substrate_name: H2O; 4-Nitrophenyl phosphate

substrate: H2O; 4-Nitrophenyl phosphate


organism: Thermus thermophilus

substrate_name: 4-nitrophenyl phosphate

substrate: 4-nitrophenyl phosphate


organism: Vibrio sp. G15-21

proof_lines: mutant Y423F, presence of 0.5 M NaCl, pH 8, 10°C


proof_lines: mutant A221D, presence of 0.5 M NaCl, pH 8, 10°C


proof_lines: mutant K422E, presence of 0.5 M NaCl, pH 8, 10°C


proof_lines: mutant K422L/Y423F, presence of 0.5 M NaCl, pH 8, 10°C


proof_lines: mutant K422L, presence of 0.5 M NaCl, pH 8, 10°C


proof_lines: mutant Y222P, presence of 0.5 M NaCl, pH 8, 10°C


proof_lines: wild-type, presence of 0.5 M NaCl, pH 8, 10°C


organism: Bacillus subtilis


substrate_name: D-Ribose 5-phosphate

substrate: D-Ribose 5-phosphate


organism: Homo sapiens

substrate_name: H2O; AMP

substrate: H2O; AMP


substrate_name: ATP; D-2'-Deoxycarbocyclic guanosine

substrate: ATP; D-2'-Deoxycarbocyclic guanosine


substrate_name: H2O; IMP

substrate: H2O; IMP


substrate_name: H2O; dUMP

substrate: H2O; dUMP


organism: Boophilus microplus


organism: Drosophila melanogaster


substrate_name: H2O; GMP

substrate: H2O; GMP


substrate_name: GMP

substrate: GMP


substrate_name: dGMP

substrate: dGMP


substrate_name: AMP; H2O

substrate: AMP; H2O


organism: Helicobacter pylori


substrate_name: Inosine

proof_lines: presence of 2 mM ATP, Tris-buffer, pH 7.4

proof_lines: presence of 0.4 mM 2,3-bisphosphoglycerate, Tris-buffer, pH 7.4

proof_lines: presence of 2 mM ATP, phosphate-buffered saline, pH 7.4

substrate: Inosine


substrate_name: CMP

substrate: CMP


substrate_name: H2O; CMP

substrate: H2O; CMP


substrate_name: AMP

substrate: AMP


organism: Mycoplasma pulmonis

substrate_name: H2O; 5'-AMP

substrate: H2O; 5'-AMP


enzyme_display_name: alpha-amylase

organism: Alteromonas haloplanktis

enzyme: alpha-amylase


organism: Anabaena sp.

substrate_name: oyster glycogen

assay_conditions_summary: pH 7; 303.15 K; buffer 10 mM MOPS

proof_lines: at 30°C in 10 mM MOPS buffer (pH 7.0) with 5 mM CaCl2

substrate: oyster glycogen


organism: Bacillus amyloliquefaciens

substrate_name: starch

substrate: starch


substrate_name: soluble starch

proof_lines:
wild-type, pH 6, 60°C
wild type enzyme

substrate: soluble starch


organism: Bacillus licheniformis

proof_lines: determined with Lineweaver-Burk plot, competitive inhibition, pH 7.4, 37°C, docking simulations show that Tris binds deep inside the active site of the enzyme


organism: Hordeum vulgare

proof_lines:
below, pH 5.5, 37°C, mutant Y105A
above, pH 5.5, 37°C, mutant Y105A


substrate_name: amylose DP17

substrate: amylose DP17


substrate_name: amylose DP440

substrate: amylose DP440


proof_lines: Y380M mutant, pH 5.5, 30°C, kcat/Km: 39 1/s/mg/ml

proof_lines: Y380M mutant, pH 5.5, 30°C, kcat/Km: 425 1/s/mg/ml


proof_lines: Y380A mutant, pH 5.5, 30°C, kcat/Km: 28 1/s/mg/ml

proof_lines: Y380A mutant, pH 5.5, 30°C, kcat/Km: 264 1/s/mg/ml


proof_lines: Y105A mutant, kcat below 10 1/s, pH 5.5, 30°C

proof_lines: Y105A mutant, pH 5.5, 30°C, kcat/Km: 132 1/s/mg/ml, kcat/Km decreases 10-25fold for all Y105 mutants

proof_lines: Y105A mutant, Km above 10 mM, kcat below 10 1/s, pH 5.5, 30°C


proof_lines: Y105A/Y380M mutant, kcat below 10 1/s, pH 5.5, 30°C

proof_lines: Y105A/Y380M mutant, pH 5.5, 30°C, kcat/Km: 56 1/s/mg/ml, kcat/Km decreases 10-25fold for all Y105 mutants

proof_lines: Y105A/Y380M mutant, Km above 10 mM, kcat below 10 1/s, pH 5.5, 30°C


proof_lines: wild-type enzyme, pH 5.5, 30°C, kcat/Km: 48 1/s/mg/ml

proof_lines: wild-type enzyme, pH 5.5, 30°C, kcat/Km: 1300 1/s/mg/ml

proof_lines: wild-type enzyme, pH 5.5, 30°C, kcat/Km: 38 1/s/mg/ml


proof_lines: Y105A/Y380A mutant, kcat below 10 1/s, pH 5.5, 30°C

proof_lines: Y105A/Y380A mutant, pH 5.5, 30°C, kcat/Km: 51 1/s/mg/ml, kcat/Km decreases 10-25fold for all Y105 mutants

proof_lines: Y105A/Y380A mutant, Km above 10 mM, pH 5.5, 30°C, kcat/Km: 1300 1/s/mg/ml


enzyme_display_name: alpha-glucosidase

organism: Apis mellifera

substrate_name: Phenyl alpha-D-glucoside

enzyme: alpha-glucosidase

substrate: Phenyl alpha-D-glucoside


substrate_name: maltopentaose

substrate: maltopentaose


substrate_name: Nigerose

substrate: Nigerose


substrate_name: turanose

substrate: turanose


substrate_name: maltotetraose

substrate: maltotetraose


organism: Aspergillus nidulans

substrate_name: H2O; Maltose

substrate: H2O; Maltose


substrate_name: alpha,alpha-Trehalose

substrate: alpha,alpha-Trehalose


substrate_name: Maltose

substrate: Maltose


substrate_name: Isomaltose

substrate: Isomaltose


substrate_name: Maltotriose

substrate: Maltotriose


organism: Apis cerana indica


substrate_name: isomaltose

substrate: isomaltose


substrate_name: 4-nitrophenyl alpha-D-glucoside

substrate: 4-nitrophenyl alpha-D-glucoside


substrate_name: sucrose

substrate: sucrose


substrate_name: p-Nitrophenyl-alpha-D-glucoside

substrate: p-Nitrophenyl-alpha-D-glucoside


substrate_name: Sucrose

substrate: Sucrose


substrate_name: Kojibiose

substrate: Kojibiose


substrate_name: Maltohexaose

substrate: Maltohexaose


organism: Sus scrofa

proof_lines: pH not specified in the publication, 25°C

proof_lines: presence of 4.8 mM neohesperidin dihydrochalcone, pH 7.2, 42°C


organism: Tepidimonas fonticaldi

substrate_name: soluble potato starch

substrate: soluble potato starch


organism: Thermobifida fusca

proof_lines: pH not specified in the publication, 55°C


organism: Thermotoga neapolitana

substrate_name: maltopentaose

substrate: maltopentaose


substrate_name: 4-nitrophenyl alpha-D-maltohexaoside

substrate: 4-nitrophenyl alpha-D-maltohexaoside


assay_conditions_summary: pH 6.5; 348.15 K; buffer 50 mM sodium phosphate

proof_lines: in 50 mM sodium phosphate buffer (pH 6.5) at 75°C


substrate_name: maltotriose

substrate: maltotriose


substrate_name: maltoheptaose

substrate: maltoheptaose


organism: Tribolium castaneum

substrate_name: amylopectin

substrate: amylopectin


organism: uncultured organism


organism: Vigna angularis

substrate_name: Amylose; H2O

substrate: Amylose; H2O


organism: Helicoverpa armigera

substrate_name: starch

substrate: starch


organism: Homo sapiens

substrate_name: Maltotriose; H2O

proof_lines:
in presence of 1 mM NO2-
in presence of 0.2 mM ClO3-
in presence of 0.01 mM I-

substrate: Maltotriose; H2O


substrate_name: Maltotriose

substrate: Maltotriose


substrate_name: amylose DP17

substrate: amylose DP17


substrate_name: Nigerose

substrate: Nigerose


organism: Bacillus sp.

substrate_name: H2O; Trehalose

substrate: H2O; Trehalose


organism: Caldanaerobacter subterraneus subsp. tengcongensis

substrate_name: 4-nitrophenyl alpha-D-glucopyranoside

substrate: 4-nitrophenyl alpha-D-glucopyranoside


organism: Culex quinquefasciatus


organism: Erwinia rhapontici

substrate_name: Maltulose

substrate: Maltulose


substrate_name: Trehalulose

substrate: Trehalulose


substrate_name: Leucrose

substrate: Leucrose


substrate_name: Sucrose

substrate: Sucrose


organism: Bacillus licheniformis

proof_lines: wild-type, kcat/Km: 140000 1/s/M, 37°C, pH 4.5

proof_lines: wild-type, kcat/Km: 770000 1/s/M, 25°C, pH 6.0

proof_lines: wild-type, kcat/Km: 870000 1/s/M, 37°C, pH 5.5


organism: Bacillus sp. dsh19-1

substrate_name: soluble starch

substrate: soluble starch


organism: Bacillus subtilis

substrate_name: H2O; o-Nitrophenyl-alpha-maltoside

substrate: H2O; o-Nitrophenyl-alpha-maltoside


organism: Bifidobacterium longum

proof_lines: recombinant His6-tagged enzyme, pH 5.0, 20°C


organism: Callosobruchus chinensis


organism: Drosophila melanogaster

proof_lines: pH not specified in the publication, 20°C


organism: Euplotes focardii


organism: Geobacillus thermoleovorans

substrate_name: raw starch

proof_lines: pH 4.0, 60°C, recombinant chimeric acidic alpha-amylase Ba-Gt-amy

substrate: raw starch


organism: Glycine max

substrate_name: H2O; Starch

substrate: H2O; Starch


organism: Hordeum vulgare

proof_lines: H395A mutant, pH 5.5, 30°C, kcat/Km: 23 1/s/mg/ml

proof_lines: H395A mutant, pH 5.5, 30°C, kcat/Km: 394 1/s/mg/ml

proof_lines: H395A mutant, kcat below 10 1/s, pH 5.5, 30°C


proof_lines: Y380A/H395A mutant, pH 5.5, 30°C, kcat/Km: 25 1/s/mg/ml

proof_lines: Y380A/H395A mutant, pH 5.5, 30°C, kcat/Km: 543 1/s/mg/ml


organism: Lactobacillus amylovorus


organism: Lactobacillus manihotivorans

substrate_name: H2O; Amylose

substrate: H2O; Amylose


organism: Lactobacillus plantarum

substrate_name: Amylose

substrate: Amylose


organism: Moneuplotes crassus

substrate_name: starch

substrate: starch


organism: Paenibacillus sp. SSG-1

substrate_name: maltopentaose

substrate: maltopentaose


substrate_name: amylose

substrate: amylose


substrate_name: maltose

substrate: maltose


substrate_name: maltoheptaose

substrate: maltoheptaose


substrate_name: maltotriose

substrate: maltotriose


organism: Priestia megaterium

assay_conditions_summary: pH 7.2; 303.15 K; buffer the assay

proof_lines: mutant enzyme A53S, in the presence of 5 mM Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C

proof_lines: mutant enzyme A53S, in the absence of Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C


proof_lines: mutant enzyme H58I, in the presence of 5 mM Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C

proof_lines: mutant enzyme H58I, in the absence of Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C


proof_lines: wild type enzyme, in the presence of 5 mM Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C

proof_lines: wild type enzyme, in the absence of Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C


organism: Pseudoalteromonas haloplanktis

proof_lines: pH not specified in the publication, 5°C

proof_lines: pH not specified in the publication, 25°C


organism: Pyrococcus sp.

substrate_name: glycogen

substrate: glycogen


organism: Ruminococcus bromii

proof_lines: recombinant His-tagged enzyme, pH 5.0, 45°C


organism: Solanum tuberosum

substrate_name: maltotetraose

substrate: maltotetraose


substrate_name: Maltotriose

substrate: Maltotriose


substrate_name: Maltohexaose

substrate: Maltohexaose


organism: Sulfolobus acidocaldarius


substrate_name: maltohexaose

substrate: maltohexaose


substrate_name: 4-nitrophenyl alpha-D-glucopyranoside

substrate: 4-nitrophenyl alpha-D-glucopyranoside


organism: Sulfurisphaera tokodaii


organism: Erwinia rhapontici

substrate_name: Maltitol

substrate: Maltitol


substrate_name: palatinose

substrate: palatinose


substrate_name: Maltose

substrate: Maltose


substrate_name: turanose

substrate: turanose


substrate_name: alpha,alpha-Trehalose

substrate: alpha,alpha-Trehalose


organism: Ferroplasma acidiphilum


substrate_name: sucrose

substrate: sucrose


organism: Mortierella alliacea


substrate_name: maltopentaose

substrate: maltopentaose


substrate_name: H2O; Maltose

substrate: H2O; Maltose


organism: Rattus norvegicus

substrate_name: H2O; p-Nitrophenyl-alpha-D-glucoside

substrate: H2O; p-Nitrophenyl-alpha-D-glucoside


substrate_name: Maltose; H2O

substrate: Maltose; H2O


substrate_name: p-Nitrophenyl-alpha-D-glucoside

substrate: p-Nitrophenyl-alpha-D-glucoside


substrate_name: maltoheptaose

substrate: maltoheptaose


substrate_name: panose

substrate: panose


substrate_name: isomaltose

substrate: isomaltose


substrate_name: maltose

substrate: maltose


substrate_name: maltotetraose

substrate: maltotetraose


organism: Thermoplasma acidophilum


substrate_name: 4-nitrophenyl alpha-D-glucopyranoside

substrate: 4-nitrophenyl alpha-D-glucopyranoside


enzyme_display_name: alpha-galactosidase

organism: Geobacillus stearothermophilus

substrate_name: 4-nitrophenyl alpha-D-galactopyranoside

enzyme: alpha-galactosidase

substrate: 4-nitrophenyl alpha-D-galactopyranoside


assay_conditions_summary: pH 6.5; 298.15 K; buffer 100 mM potassium phosphate

proof_lines: mutant enzyme W336A of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C


substrate_name: raffinose

substrate: raffinose


proof_lines: mutant enzyme W336N of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C


proof_lines: mutant enzyme W336S of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C


proof_lines: wild type enzyme isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C

proof_lines: wild type enzyme isoform AgaB, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C


substrate_name: kojibiose

substrate: kojibiose


substrate_name: maltotriose

substrate: maltotriose


organism: Thermotoga maritima

substrate_name: p-Nitrophenyl-alpha-D-glucoside; H2O

substrate: p-Nitrophenyl-alpha-D-glucoside; H2O


organism: Thermus thermophilus

substrate_name: Isomaltose

substrate: Isomaltose


substrate_name: H2O; alpha,alpha-Trehalose

substrate: H2O; alpha,alpha-Trehalose


substrate_name: alpha,alpha-Trehalose

substrate: alpha,alpha-Trehalose


substrate_name: Maltose

substrate: Maltose


organism: Bacteroides fragilis

substrate_name: p-Nitrophenyl-alpha-D-galactoside

substrate: p-Nitrophenyl-alpha-D-galactoside


substrate_name: H2O; Gal-alpha1->3(Fuc-alpha1->2)Gal

substrate: H2O; Gal-alpha1->3(Fuc-alpha1->2)Gal


organism: Bacteroides thetaiotaomicron

substrate_name: Melibiose

substrate: Melibiose


substrate_name: H2O; p-Nitrophenyl-alpha-D-galactoside

substrate: H2O; p-Nitrophenyl-alpha-D-galactoside


substrate_name: 4-nitrophenyl alpha-D-galactoside

substrate: 4-nitrophenyl alpha-D-galactoside


assay_conditions_summary: pH 6; 308.15 K; buffer 40 mM sodium phosphate

proof_lines: mutant enzyme E470Q, in 40 mM sodium phosphate buffer, pH 6.0, at 35°C


proof_lines: mutant enzyme D415N, in 40 mM sodium phosphate buffer, pH 6.0, at 35°C


proof_lines: wild type enzyme, in 40 mM sodium phosphate buffer, pH 6.0, at 35°C


organism: Bifidobacterium breve


organism: Fusarium sp.


proof_lines:
37°C, pH 5.0, wild-type enzyme
pH 5.0, 65°C, wild-type enzyme


organism: Rhizomucor miehei

substrate_name: stachyose

substrate: stachyose


substrate_name: melibiose

substrate: melibiose


organism: Saccharolobus solfataricus

substrate_name: p-nitrophenyl alpha-D-galactopyranoside

assay_conditions_summary: pH 5; 353.15 K; buffer 50 mM citric acid-Na2HPO4

proof_lines: in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C

substrate: p-nitrophenyl alpha-D-galactopyranoside


organism: Saccharomyces cerevisiae


assay_conditions_summary: pH 7.4; 303.15 K; buffer condition


organism: Saccharopolyspora erythraea


organism: Sphingomonas sp.


organism: Sulfolobus solfataricus


organism: Thermothielavioides terrestris

substrate_name: 4-nitrophenyl alpha-D-galactopyranoside

substrate: 4-nitrophenyl alpha-D-galactopyranoside


substrate_name: 4-O-methylphenyl alpha-D-galactoside

assay_conditions_summary: pH 5; 310.15 K; buffer condition

proof_lines: wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C

substrate: 4-O-methylphenyl alpha-D-galactoside


substrate_name: 4-bromophenyl alpha-D-galactoside

proof_lines: mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C

substrate: 4-bromophenyl alpha-D-galactoside


proof_lines: pH 5.0, 65°C, mutant enzyme D327G, assay performed in 500 mM NaN3


organism: Kocuria sp. Mn22


organism: Luteimicrobium xylanilyticum

substrate_name: beechwood xylan

substrate: beechwood xylan


organism: Malbranchea cinnamomea

substrate_name: oat spelt xylan

substrate: oat spelt xylan


organism: Massilia sp. RBM26


organism: Neocallimastix patriciarum

assay_conditions_summary: pH 6; 323.15 K; buffer notes) and the accession is annotated bifunctional

proof_lines: in 12 mM sodium citrate, 50 mM sodium phosphate buffer (pH 6.5)


organism: Nesterenkonia xinjiangensis

proof_lines: pH 7.0, 55°C, recombinant enzyme, birchwood xylan

proof_lines: pH 7.0, 55°C, recombinant enzyme, oat spelt xylan


organism: Niallia circulans

proof_lines: recombinant wild-type enzyme, pH 7.2, 22°C


organism: Penicillium citrinum

substrate_name: birchwood xylan

substrate: birchwood xylan


organism: Penicillium sp. CGMCC 1669


organism: Pseudoalteromonas haloplanktis

substrate_name: xylan

substrate: xylan


organism: Pseudopedobacter saltans

proof_lines: His-tagged enzyme, pH 6.0, 40°C, substrate xylan MW 20000-30000


organism: Teredinibacter turnerae


organism: Thermoanaerobacterium saccharolyticum


organism: Thermochaetoides thermophila

proof_lines: recombinant His-tagged enzyme, pH 5.0, 55°C


organism: Thermomyces lanuginosus


organism: Thermothelomyces thermophilus

substrate_name: Remazol brilliant blue R 4-O-methyl-D-glucuronoxylan; H2O


substrate: Remazol brilliant blue R 4-O-methyl-D-glucuronoxylan; H2O


organism: Thermotoga maritima


proof_lines: mutant enzyme N110D/Q111Y, at pH 5.0 and 80°C


organism: Trichoderma reesei

proof_lines:
pH 5.0, 50°C, birchwood xylan
pH 5.0, 50°C, beechwood xylan
pH 5.0, 50°C, oat spelt xylan


organism: uncultured bacterium

substrate_name: oat-spelt xylan

substrate: oat-spelt xylan


proof_lines:
Raw SKiD Env: Temp=65°C, pH=5
Raw SKiD Env: Temp=37°C, pH=5


organism: Thermus sp. T2


organism: Alicyclobacillus sp.

substrate_name: xylotetraose

substrate: xylotetraose


organism: Aspergillus sulphureus

proof_lines: recombinant wild-type enzyme, pH 5.0, 50°C, oat spelt xylan


organism: Bacillus circulans

substrate_name: H2O; 1,4-beta-D-Xylan

unit: l/(min*g)

substrate: H2O; 1,4-beta-D-Xylan


organism: Bacillus subtilis


proof_lines: chimera carrying a carbohydrate-binding module, pH 6.0, 50°C


proof_lines:
wild-type, pH 6.0, 50°C
wild-type


organism: Bacillus thermodenitrificans

substrate_name: 1,4-beta-D-Xylan; H2O

substrate: 1,4-beta-D-Xylan; H2O


organism: Cellvibrio japonicus

substrate_name: H2O; 4-Nitrophenyl-beta-D-xylobioside

substrate: H2O; 4-Nitrophenyl-beta-D-xylobioside


organism: Fusarium oxysporum f. sp. vasinfectum

proof_lines:
pH 8.0, 40°C
Raw SKiD Env: Temp=40°C, pH=8


organism: Geobacillus stearothermophilus


organism: Hordeum vulgare

assay_conditions_summary: pH 5; 313.15 K; buffer citric acid and disodium hydrogen phosphate

proof_lines: in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C


organism: Pseudothermotoga thermarum


organism: Rhizophlyctis rosea

proof_lines: pH 7, 50°C, large enzyme form RrXyn11A L

proof_lines: pH 7, 40°C, small enzyme form RrXyn11A S


organism: Rhodothermaceae bacterium RA

assay_conditions_summary: pH 8


organism: Scheffersomyces stipitis


organism: Sphingobacterium sp. TN19


organism: Streptomyces fradiae


organism: Streptomyces sp. TN119


organism: Talaromyces funiculosus


proof_lines: apparent value for the recombinant enzyme


organism: Talaromyces pinophilus

substrate_name: wheat arabinoxylan

substrate: wheat arabinoxylan


organism: Teredinibacter turnerae

substrate_name: marine beta-1,3-beta-1,4-xylan

proof_lines: pH 6.0, 37°C, recombinant enzyme mutant D281N

substrate: marine beta-1,3-beta-1,4-xylan


substrate_name: arabinoxylan

proof_lines: pH 6.0, 37°C, recombinant wild-type enzyme

substrate: arabinoxylan


proof_lines: mutant enzyme W336F of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C


proof_lines: mutant enzyme W336D of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C


substrate_name: raffinose

substrate: raffinose


substrate_name: 4-nitrophenyl alpha-D-galactopyranoside

substrate: 4-nitrophenyl alpha-D-galactopyranoside


organism: Homo sapiens


organism: Mus musculus


organism: Phanerodontia chrysosporium

substrate_name: 1-naphthyl alpha-D-galactoside

assay_conditions_summary: pH 5

proof_lines: temperature not specified in the publication, pH 5

substrate: 1-naphthyl alpha-D-galactoside


organism: Pseudoalteromonas sp. KMM 701

substrate_name: Unknown

substrate: Unknown


organism: Rhizomucor miehei


substrate_name: melibiose

substrate: melibiose


enzyme_display_name: subtilisin

organism: Bacillus lentus

substrate_name: Peptidyl-p-nitroanilide

enzyme: subtilisin

substrate: Peptidyl-p-nitroanilide


organism: Bacillus amyloliquefaciens

substrate_name: N-Acetyl-L-tyrosine methyl ester

substrate: N-Acetyl-L-tyrosine methyl ester


substrate_name: N-Acetyl-L-tryptophan methyl ester

substrate: N-Acetyl-L-tryptophan methyl ester


substrate_name: Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide

substrate: Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide


enzyme_display_name: peptidyl-dipeptidase A

organism: Oryctolagus cuniculus

substrate_name: Hip-His-Leu

assay_conditions_summary: pH 8.3; 310.15 K; buffer sodium borate

enzyme: peptidyl-dipeptidase A

substrate: Hip-His-Leu


substrate_name: Angiotensin I

substrate: Angiotensin I


substrate_name: Angiotensin I; H2O

proof_lines: pH 7.5, temperature not specified in the publication

proof_lines: pH and temperature not specified in the publication

substrate: Angiotensin I; H2O


organism: Struthio camelus australis


substrate_name: N-Acetyl-L-phenylalanine methyl ester

substrate: N-Acetyl-L-phenylalanine methyl ester


organism: uncultured bacterium

substrate_name: oat-spelt xylan

substrate: oat-spelt xylan


substrate_name: beechwood xylan

proof_lines:
mutant XylA, pH 6.0, 50°C
mutant XylB, pH 6.0, 50°C

substrate: beechwood xylan


organism: Drosophila melanogaster


substrate_name: Hip-His-Leu; H2O

proof_lines: C-catalytic domain of ACE, pH 7.5, temperature not specified in the publication

substrate: Hip-His-Leu; H2O


substrate_name: N-Acetyl-Ser-Asp-Lys-Pro

substrate: N-Acetyl-Ser-Asp-Lys-Pro


substrate_name: Leu-enkephalin

substrate: Leu-enkephalin


substrate_name: Leu-enkephalinamide

substrate: Leu-enkephalinamide


substrate_name: H2O; Hip-His-Leu

substrate: H2O; Hip-His-Leu


substrate_name: Peptidyl-p-nitroanilide; H2O

substrate: Peptidyl-p-nitroanilide; H2O


organism: Bacillus licheniformis


substrate_name: N-Acetyl-L-tryptophan methyl ester; H2O

substrate: N-Acetyl-L-tryptophan methyl ester; H2O


organism: Bacillus subtilis


substrate_name: furanacryloyl-L-Phe-Gly-Gly

substrate: furanacryloyl-L-Phe-Gly-Gly


proof_lines: 37°C, pH 8.3, chimeric enzyme C417-579Ndom-ACE


substrate_name: Succinyl-Ala-Ala-Pro-Ala-p-nitroanilide

substrate: Succinyl-Ala-Ala-Pro-Ala-p-nitroanilide


substrate_name: Succinyl-Ala-Ala-Pro-Met-p-nitroanilide

substrate: Succinyl-Ala-Ala-Pro-Met-p-nitroanilide


substrate_name: Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide

substrate: Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide


organism: Plasmodium falciparum

substrate_name: Ac-LVSADNIDIS; H2O

substrate: Ac-LVSADNIDIS; H2O


enzyme_display_name: arginase

substrate_name: L-arginine

enzyme: arginase

substrate: L-arginine


substrate_name: L-Arginine; H2O

substrate: L-Arginine; H2O


proof_lines: mutant S230G, activated by Co2+, pH 7.4, 37°C

proof_lines: mutant S230G, activated by Mn2+, pH 7.4, 37°C

proof_lines: product inhibition constant, Mn2+-activated mutant S230G, pH 7.4, 37°C


proof_lines: mutant S230C, activated by Mn2+, pH 7.4, 37°C

proof_lines: mutant S230C, activated by Co2+, pH 7.4, 37°C


proof_lines: mutant D181N/S230G, activated by Co2+, pH 7.4, 37°C

proof_lines: mutant D181N/S230G, activated by Mn2+, pH 7.4, 37°C

proof_lines: product inhibition constant, Co2+-activated mutant D181N/S230G, pH 7.4, 37°C


proof_lines: mutant D181S, activated by Co2+, pH 7.4, 37°C


proof_lines: mutant D181S/S230G, activated by Co2+, pH 7.4, 37°C


proof_lines: mutant S230T, activated by Co2+, pH 7.4, 37°C


proof_lines: mutant S230D, activated by Co2+, pH 7.4, 37°C


proof_lines: mutant D181E/S230A, activated by Co2+, pH 7.4, 37°C


proof_lines: mutant D181E, activated by Co2+, pH 7.4, 37°C


proof_lines: mutant D181N, activated by Mn2+, pH 7.4, 37°C

proof_lines: mutant D181N, activated by Co2+, pH 7.4, 37°C

proof_lines: product inhibition constant, Co2+-activated mutant D181N, pH 7.4, 37°C


assay_conditions_summary: pH 8; 298.15 K; buffer phosphate


proof_lines:
recombinant enzyme, 5°C, pH 8.6
recombinant enzyme, 45°C, pH 8.6
recombinant enzyme, 25°C, pH 8.6


proof_lines: arginase 2, pH and temperature not specified in the publication


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme N130D/S137CN139D

proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme N130D/S137C/N139D


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme D181T


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme N130D/N139D


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme N130D


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme I129T/N130Y/T131A


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme V182E


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme S137C


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme D181T/V182E


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme S137C/N139D


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme N139D


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme N130D/S137C


organism: Bacillus brevis

substrate_name: H2O; L-Arginine

substrate: H2O; L-Arginine


organism: Bacillus caldovelox


organism: [Bacillus] caldovelox


proof_lines:
at pH 8.4 and 45°C
Raw SKiD Env: Temp=45°C, pH=8.4


organism: Bos taurus


organism: Rattus norvegicus


proof_lines: T135S mutant, kcat is unchanged relatively to values measured for the wild-type enzyme

proof_lines: T135A mutant, is reduced 26% relative to values measured for the wild-type enzyme


substrate_name: Unknown

substrate: Unknown


organism: Saccharomyces cerevisiae


organism: Schistosoma mansoni


organism: Solanum lycopersicum


organism: Sulfobacillus acidophilus


organism: Sus scrofa


organism: Geobacillus thermodenitrificans


organism: Glaciozyma antarctica


organism: Helicobacter pylori


organism: Heteropneustes fossilis


organism: Homo sapiens


proof_lines: pH 9.5, temperature not specified in the publication, wild-type enzyme


organism: Plasmodium falciparum 3D7

proof_lines: mutant E295A/R404A, pH 8.0, temperature not specified in the publication


proof_lines: mutant E295R, pH 8.0, temperature not specified in the publication


proof_lines: mutant R404A, pH 8.0, temperature not specified in the publication


proof_lines: wild-type, pH 8.0, temperature not specified in the publication


proof_lines:
mutant dN-PFA, pH 7.4, 37°C
mutant dN-PFA, pH 8.0, 37°C
mutant dN-PFA, pH 8.5, 37°C


proof_lines:
Raw SKiD Env: Temp=37°C, pH=7.4
Raw SKiD Env: Temp=37°C, pH=8
Raw SKiD Env: Temp=37°C, pH=8.5


assay_conditions_summary: pH 6


organism: Leishmania amazonensis


organism: Leishmania donovani


organism: Leishmania infantum


organism: Leishmania mexicana


organism: Mus musculus


organism: Neurospora crassa


organism: Oryza sativa


organism: Plasmodium falciparum


proof_lines: mutant E295A, pH 8.0, temperature not specified in the publication


enzyme_display_name: orotidine-5'-phosphate decarboxylase

organism: Methanothermobacter thermautotrophicus

substrate_name: orotidine 5'-phosphate

proof_lines:
above 10 mM
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=25°C, pH=7.1

enzyme: orotidine-5'-phosphate decarboxylase

substrate: orotidine 5'-phosphate


organism: Xenopus laevis


organism: Escherichia coli

substrate_name: Orotidine 5'-phosphate

substrate: Orotidine 5'-phosphate


organism: Methanobacterium thermoautotrophicus


enzyme_display_name: dihydrolipoyl dehydrogenase

organism: Unknown

substrate_name: H2O; 2-Oxoglutarate; Ferricyanide

assay_conditions_summary: cofactor NAD+

enzyme: dihydrolipoyl dehydrogenase

substrate: H2O; 2-Oxoglutarate; Ferricyanide


substrate_name: NAD+; Dihydrolipoamide

assay_conditions_summary: cofactor NADP+

substrate: NAD+; Dihydrolipoamide


enzyme_display_name: aspartate transaminase

organism: Lupinus angustifolius

proof_lines:
isozyme AAT-P1, pH 7.6, 25°C
isozyme AAT-P2, pH 7.6, 25°C

enzyme: aspartate transaminase


organism: Medicago sativa

substrate_name: oxaloacetate

proof_lines: isozyme AAT-2, including forms 2a, 2b and 2c, pH 8.0

substrate: oxaloacetate


organism: Oryctolagus cuniculus

proof_lines:
cytosolic isozyme, pH 8.0, 37°C
mitochondrial isozyme, pH 8.0, 37°C


substrate_name: L-aspartate

proof_lines:
isoenzyme AAT-1, pH 7.8, 37°C
isoenzyme AAT-2
isoenzyme AAT-2, pH 7.8, 37°C

substrate: L-aspartate


organism: Ovis aries

proof_lines:
pH 7.4, 25°C
mitochondrial isozyme, pH 7.4, 25°C
cytosolic isozyme, pH 7.4, 25°C


organism: Panicum miliaceum

proof_lines: isozyme cAspAT, alpha-form, pH 8.0, 25°C

proof_lines: isozyme cAspAT, gamma-form, pH 8.0, 25°C

proof_lines: isozyme pAsp-AT, beta-form, pH 8.0, 25°C


organism: Rattus norvegicus

proof_lines: enzyme from nonsynaptic mitochondrion, pH 7.8, 30°C


organism: Sus scrofa


enzyme_display_name: branched-chain-amino-acid transaminase

organism: Canis lupus familiaris

proof_lines: pH 8.0, 37°C, isoenzyme III, leucine as amino group donor

proof_lines: pH 8.0, 37°C, isoenzyme I, isoleucine as amino group donor

proof_lines: pH 8.0, 37°C, isoenzyme III, 2-oxoglutarate as amino group acceptor

enzyme: branched-chain-amino-acid transaminase


organism: Homo sapiens

substrate_name: L-Glutamate; 4-Methyl-2-oxopentanoate

proof_lines: TAT-peptide fusion protein, pH 7.4, 25°C

proof_lines: pH 8.4, 25°C, substrate L-alloisoleucine

proof_lines: pH 8.0, 37°C, isoenzyme III, valine as amino group donor

substrate: L-Glutamate; 4-Methyl-2-oxopentanoate


organism: Saccharomyces cerevisiae

substrate_name: beta-D-fructose 6-phosphate

assay_conditions_summary: pH 7.1; 303.15 K; cofactor ATP

proof_lines: pH 7.1, 30°C, kinetic data of various enzyme forms

substrate: beta-D-fructose 6-phosphate


assay_conditions_summary: cofactor ATP


enzyme_display_name: (S)-hydroxynitrile lyase

organism: Hevea brasiliensis

substrate_name: acetone cyanohydrin

proof_lines:
pH 5.4
isoform HNL, at pH 7.2 and 25°C
Raw SKiD Env: Temp=-----, pH=-----

enzyme: (S)-hydroxynitrile lyase

substrate: acetone cyanohydrin


organism: Manihot esculenta

substrate_name: Acetone cyanohydrin

substrate: Acetone cyanohydrin


substrate_name: Mandelonitrile

substrate: Mandelonitrile


substrate_name: (S)-mandelonitrile

assay_conditions_summary: pH 5; 296.15 K; buffer pH settings and a separate pH

proof_lines: wild type enzyme, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication

proof_lines: wild type enzyme, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication

substrate: (S)-mandelonitrile


assay_conditions_summary: pH 5; buffer pH above 6

proof_lines: mutant enzyme H103L, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication

proof_lines: mutant enzyme H103L, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication


substrate_name: 2-thiophene carboxaldehyde

substrate: 2-thiophene carboxaldehyde


substrate_name: 4-biphenyl carboxaldehyde

substrate: 4-biphenyl carboxaldehyde


substrate_name: 1-naphthalene carboxaldehyde

substrate: 1-naphthalene carboxaldehyde


organism: Plasmodium falciparum

substrate_name: NAD+

assay_conditions_summary: pH 8; 298.15 K; cofactor NAD+

proof_lines: pH 8.0, 25°C, recombinant His-tagged enzyme

proof_lines: pH 7.0, 25°C, recombinant His-tagged enzyme

proof_lines: pH 8.0, 25°C, mitochondrial isozyme, forward reaction, varying conditions

substrate: NAD+


enzyme_display_name: sulfite oxidase

substrate_name: sulfite

enzyme: sulfite oxidase

substrate: sulfite


substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


organism: Zea mays

substrate_name: shikimate 3-phosphate

proof_lines:
isoenzyme II
isoenzyme I
isoenzyme II, vs. shikimate 3-phosphate

substrate: shikimate 3-phosphate


organism: Arabidopsis thaliana

proof_lines:
isozyme AAT2, pH 8.0, 25°C
isozyme AAT5, pH 8.0, 25°C
isozyme AAT1, pH 8.0, 25°C


organism: Atriplex spongiosa

proof_lines:
isozyme Asp-DEAE-1, pH 8.0
isozyme Asp-DEAE-2, pH 8.0


proof_lines:
cytosolic isozyme
mitochondrial isozyme


organism: Eleusine coracana

proof_lines:
isozyme AspAT-3, 25°C, pH 8.0
isozyme AspAT-1, 25°C, pH 8.0


organism: Gallus gallus

proof_lines: recombinant premature isozyme pmAspAT, pH 7.5, 25°C

proof_lines: recombinant isozyme mAspAT, pH 7.5, 25°C


organism: Hyalomma dromedarii


organism: Lupinus albus

substrate_name: L-Aspartate; 2-Oxoglutarate

proof_lines: isozyme AAT2, competitive versus 2-oxoglutarate

substrate: L-Aspartate; 2-Oxoglutarate


organism: Baliospermum montanum


substrate_name: (R)-mandelonitrile

substrate: (R)-mandelonitrile


substrate_name: (S)-Mandelonitrile

substrate: (S)-Mandelonitrile


substrate_name: Cyanide

smiles: C#N

substrate: Cyanide


smiles: CC(C)(O)C#N


organism: Unknown

substrate_name: Glp-Ala-Ala-Leu-p-Nitroanilide; H2O

substrate: Glp-Ala-Ala-Leu-p-Nitroanilide; H2O


substrate_name: L-arginine

substrate: L-arginine


substrate_name: fructose 6-phosphate

source_db: uniprot

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

substrate: fructose 6-phosphate

source: uniprot


organism: Rattus norvegicus

substrate_name: beta-D-fructose 6-phosphate

substrate: beta-D-fructose 6-phosphate


enzyme_display_name: acetate kinase

organism: Methanosarcina thermophila

substrate_name: ATP

enzyme: acetate kinase

substrate: ATP


organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)


organism: Bordetella pertussis (strain ATCC 9797 / DSM 5571 / CCUG 30873 / LMG 14455 / NCTC 10739 / 18323)


organism: Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM)

substrate_name: AMP (at pH 7.5)

assay_conditions_summary: pH 7.5; cofactor ATP

substrate: AMP (at pH 7.5)


organism: Escherichia coli (strain K12)

substrate_name: ATP (at pH 7.4 and 30 degrees Celsius)

assay_conditions_summary: pH 7.4; cofactor ATP

substrate: ATP (at pH 7.4 and 30 degrees Celsius)


organism: Homo sapiens

substrate_name: AMP (with ATP as phosphate donor)

substrate: AMP (with ATP as phosphate donor)


substrate_name: AMP

substrate: AMP


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)


organism: Spirochaeta sp.

substrate_name: acetate

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

proof_lines:
pH 7.5, 30°C, isoenzyme II
pH 7.5, 30°C, isoenzyme I

substrate: acetate


organism: Mus musculus

proof_lines: isoenzyme AK1b, membrane protein fraction

proof_lines: isoenzyme AK1, membrane protein fraction

proof_lines: isoenzyme AK1, cytosolic protein fraction


organism: Aspergillus oryzae

substrate_name: p-nitrophenyl phosphate

proof_lines:
isoenzyme III
isoenzyme I
isoenzyme II

substrate: p-nitrophenyl phosphate


substrate_name: H2O; 4-Nitrophenyl phosphate

proof_lines: allozyme D, pH 7.5, 37°C, in presence of Mg2+ and Zn2+

proof_lines: allozyme D, pH 9.8, in presence of Mg2+ and Zn2+

substrate: H2O; 4-Nitrophenyl phosphate


organism: Bos taurus

substrate_name: H2O; AMP

proof_lines:
isoenzyme 5-NT-3
isoenzyme 5-NT-1
isoenzyme 5-NT-2 and 5-NT-4

substrate: H2O; AMP


organism: Sus scrofa

proof_lines:
25°C, pH 7.2
isozyme PPA-II, at pH 6.9 and 30°C
isozyme PPA-I, at pH 6.9 and 30°C


organism: Aspergillus niger

substrate_name: H2O; Raffinose

proof_lines:
pH 4.5, 50°C, isoenzyme alpha-Gal I
pH 4.5, 50°C, isoenzyme alpha-Gal II
pH 4.5, 50°C, isoenzyme alpha-Gal III

substrate: H2O; Raffinose


organism: Phlebia radiata

substrate_name: p-nitrophenyl alpha-D-galactopyranoside

proof_lines:
isoenzyme AgaS-m2
isoenzyme AgaS-b2
isoenzyme AgaS-b3

substrate: p-nitrophenyl alpha-D-galactopyranoside


substrate_name: raffinose

substrate: raffinose


organism: Melanocarpus albomyces

substrate_name: birchwood xylan

proof_lines:
isozyme IIa, pH 6.0, 70°C
isozyme Ia, pH 6.0, 70°C
isozyme IId, pH 6.0, 70°C

substrate: birchwood xylan


organism: Trichoderma sp.

proof_lines: pH 5.6, 50°C, isozyme EX1, oat spelt xylan

proof_lines: pH 5.6, 50°C, isozyme EX1, beechwood xylan

proof_lines: pH 5.6, 50°C, isozyme EX2, birchwood xylan


enzyme_display_name: cysteine synthase

organism: Arabidopsis thaliana

substrate_name: O-acetylserine (at pH 7.0 and 25 degrees Celsius) for the cysteine synthase activity

enzyme: cysteine synthase


substrate_name: L-cysteine for the cystathionine gamma-lyase activity


organism: Caenorhabditis elegans

substrate_name: O-acetylserine

substrate: O-acetylserine


substrate_name: O-acetylserine (at 25 degrees Celsius and pH 7.5)


substrate: O-acetylserine (at 25 degrees Celsius and pH 7.5)


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: O-acetyl-L-homoserine (OAH) (at 30 degrees Celsius in potassium phosphate buffer)

assay_conditions_summary: buffer potassium phosphate


substrate_name: O-acetyl-L-homoserine

substrate: O-acetyl-L-homoserine


organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

substrate_name: O-acetyl-L-serine as isolated subunit and in complex with CysK


organism: Solanum tuberosum

substrate_name: L-cysteine for the L-3-cyanoalanine synthase activity (in the presence of 5 mM KCN)


substrate_name: O(3)-acetyl-L-serine for the cysteine synthase activity (in the presence of 2.5 mM Na(2)S)


organism: Spinacia oleracea

substrate_name: O(3)-acetyl-L-serine for the cysteine synthase activity with the recombinant enzyme


organism: Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534)

substrate_name: lipoate

assay_conditions_summary: cofactor NADH

substrate: lipoate


organism: Hymenolepis diminuta

substrate_name: NADH for the NADH -> NAD reaction

substrate: NADH for the NADH -> NAD reaction


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: NAD(+)

substrate: NAD(+)


organism: Thermus scotoductus (strain ATCC 700910 / SA-01)

substrate_name: Cr(VI)

substrate: Cr(VI)


enzyme_display_name: thymidylate synthase

substrate_name: dUMP

enzyme: thymidylate synthase

substrate: dUMP


enzyme_display_name: glycine hydroxymethyltransferase

substrate_name: L-serine (at pH 6.5 and 30 degrees Celsius)

enzyme: glycine hydroxymethyltransferase


substrate: L-serine (at pH 6.5 and 30 degrees Celsius)


organism: Homo sapiens

substrate_name: L-serine (with HEPES)

substrate: L-serine (with HEPES)


substrate_name: L-serine

substrate: L-serine


organism: Oryctolagus cuniculus

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


organism: Ovis aries


enzyme_display_name: glycogen phosphorylase

organism: Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C)

substrate_name: maltoheptaose (at 60 degrees Celsius)

enzyme: glycogen phosphorylase

substrate: maltoheptaose (at 60 degrees Celsius)


organism: Rhizobium meliloti

substrate_name: aspartate

substrate: aspartate


substrate_name: L-aspartate

substrate: L-aspartate


substrate_name: 4-methyl-2-oxopentanoate (4MOP)

substrate: 4-methyl-2-oxopentanoate (4MOP)


substrate_name: L-leucine

km_unit: mM

data_origin: experimental

substrate: L-leucine


organism: Bacillus subtilis (strain 168)

substrate_name: isoleucine (with 10 mM ketomethiobutyrate)


substrate: isoleucine (with 10 mM ketomethiobutyrate)


organism: Humulus lupulus

substrate_name: glutamate

assay_conditions_summary: cofactor CoA

substrate: glutamate


organism: Rattus norvegicus


organism: Arabidopsis thaliana

substrate_name: fructose-6-phosphate for the 6-phosphofructo-2-kinase activity (at pH 6.0 and 25 degrees Celsius)


substrate_name: beta-D-fructose 6-phosphate

substrate: beta-D-fructose 6-phosphate


enzyme_display_name: alkaline phosphatase

organism: Elizabethkingia meningoseptica

substrate_name: 4-nitrophenyl phosphate

enzyme: alkaline phosphatase

substrate: 4-nitrophenyl phosphate


organism: Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805)


substrate_name: myo-inositol phosphate

substrate: myo-inositol phosphate


organism: Bos taurus

substrate_name: IMP (in absence of allosteric activator)


organism: Escherichia coli (strain K12)


substrate_name: UMP (at pH 7 and at 25 degrees Celsius)

assay_conditions_summary: pH 7

substrate: UMP (at pH 7 and at 25 degrees Celsius)


substrate_name: IMP (with manganese ions as cofactor and at pH 9)

assay_conditions_summary: pH 9


organism: Gallus gallus

substrate_name: IMP

substrate: IMP


substrate_name: AMP

substrate: AMP


substrate_name: IMP (in presence of the allosteric activator ATP)


substrate_name: m(7)GMP (at 37 degrees Celsius)

substrate: m(7)GMP (at 37 degrees Celsius)


enzyme_display_name: adenylate kinase

organism: Sus scrofa

substrate_name: ThDP

assay_conditions_summary: cofactor ADP

enzyme: adenylate kinase

substrate: ThDP


substrate_name: NMN

substrate: NMN


organism: Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)


substrate_name: beta-nicotinamide D-ribonucleotide

substrate: beta-nicotinamide D-ribonucleotide


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: ATP

substrate: ATP


substrate_name: nicotinamide mononucleotide

substrate: nicotinamide mononucleotide


organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

substrate_name: ribosylnicotinamide

substrate: ribosylnicotinamide


enzyme_display_name: acetylcholinesterase

organism: Bungarus fasciatus

substrate_name: acetylcholine (at pH 7.5)

enzyme: acetylcholinesterase

substrate: acetylcholine (at pH 7.5)


organism: Caenorhabditis elegans

substrate_name: acetylthiocholine

substrate: acetylthiocholine


organism: Danio rerio

substrate_name: acetylcholine

substrate: acetylcholine


organism: Spinacia oleracea

substrate_name: O-acetylserine

substrate: O-acetylserine


organism: Streptomyces lavendulae

substrate_name: OAS

substrate: OAS


enzyme_display_name: protein farnesyltransferase

substrate_name: CVIM substrate and for farnesyl transferase activity

enzyme: protein farnesyltransferase


substrate_name: L-aspartate

substrate: L-aspartate


substrate_name: aspartate for the asparte aminotransferase activity


organism: Comamonas testosteroni


organism: Klebsiella pneumoniae

substrate_name: tryptophan (at pH 7.4 and 10 mM KMTB)

substrate: tryptophan (at pH 7.4 and 10 mM KMTB)


organism: Mus musculus


organism: Petunia hybrida

substrate_name: prephenate (with 20 mM aspartate as cosubstrate)


organism: Pinus pinaster

substrate_name: aspartate

substrate: aspartate


organism: Pseudomonas sp


organism: Rattus norvegicus

substrate_name: L-cysteine

substrate: L-cysteine


organism: Saccharomyces cerevisiae

substrate_name: orotidine 5'-phosphate

substrate: orotidine 5'-phosphate


proof_lines:
pH 7.1, 25°C, mutant Y217F
mutant Y217F, pH 7.1, 25°C


substrate_name: 5-Fluoroorotidine 5'-phosphate

substrate: 5-Fluoroorotidine 5'-phosphate


enzyme_display_name: hypoxanthine phosphoribosyltransferase

organism: Homo sapiens

substrate_name: IMP

enzyme: hypoxanthine phosphoribosyltransferase

substrate: IMP


substrate_name: hypoxanthine

substrate: hypoxanthine


organism: Agrobacterium sp. (strain CP4)

substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


organism: Escherichia coli (strain K12)

substrate_name: S3P (at pH 7 and 25 degrees Celsius)

substrate: S3P (at pH 7 and 25 degrees Celsius)


organism: Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)

substrate_name: PEP (with 100 mM ammonium at pH 7 and 25 degrees Celsius)

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


substrate_name: FPP (at pH 7.5 and at 25 degrees Celsius)


organism: Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)

substrate_name: FPP

substrate: FPP


organism: Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)

substrate_name: O(3)-acetyl-L-serine (in the presence of 1 mM sodium sulfide at pH 6.7 and 60 degrees Celsius)


organism: Arabidopsis thaliana

substrate_name: O(3)-acetyl-L-serine for the cysteine synthase activity


substrate: O(3)-acetyl-L-serine for the cysteine synthase activity


organism: Methanothermobacter thermautotrophicus


substrate_name: Orotidine 5'-phosphate

substrate: Orotidine 5'-phosphate


organism: Baliospermum montanum


substrate_name: Undecanal

smiles: CCCCCCCCCCC=O

substrate: Undecanal


substrate_name: piperonal

substrate: piperonal


substrate_name: Benzaldehyde; Cyanide

substrate: Benzaldehyde; Cyanide


substrate_name: benzaldehyde

substrate: benzaldehyde


substrate_name: Isophthaldehyde

substrate: Isophthaldehyde


smiles: CC(=O)CCC(C)C


substrate_name: Heptanal

smiles: CCCCCCC=O

substrate: Heptanal


substrate_name: Terephthalaldehyde

substrate: Terephthalaldehyde


substrate_name: Mandelonitrile

substrate: Mandelonitrile


smiles: CCC/C=C/C=O


substrate_name: 1-naphthalene carboxaldehyde

substrate: 1-naphthalene carboxaldehyde


substrate_name: 2-thiophene carboxaldehyde

substrate: 2-thiophene carboxaldehyde


proof_lines:
pH 4.2, 22°C
pH 6.0, 25°C
Raw SKiD Env: Temp=22°C, pH=4.2


substrate_name: (R)-mandelonitrile

substrate: (R)-mandelonitrile


substrate_name: 5-Fluoroorotidine 5'-phosphate

substrate: 5-Fluoroorotidine 5'-phosphate


proof_lines:
pH 7.4, 25°C
Raw SKiD Env: Temp=25°C, pH=7.2
Raw SKiD Env: Temp=25°C, pH=7.1


enzyme_display_name: phosphoenolpyruvate carboxykinase (GTP)

organism: Ascaris suum

substrate_name: Phosphoenolpyruvate; IDP; CO2

enzyme: phosphoenolpyruvate carboxykinase (GTP)

substrate: Phosphoenolpyruvate; IDP; CO2


organism: Gallus gallus

substrate_name: Phosphoenolpyruvate; CO2; IDP

substrate: Phosphoenolpyruvate; CO2; IDP


substrate_name: CO2; Phosphoenolpyruvate; GDP

proof_lines:
CO2 in form of HCO3-
Raw SKiD Env: Temp=-----, pH=-----

substrate: CO2; Phosphoenolpyruvate; GDP


organism: Mus musculus

substrate_name: ITP; Oxaloacetate

substrate: ITP; Oxaloacetate


organism: Mycobacterium smegmatis


substrate_name: GTP; Oxaloacetate

substrate: GTP; Oxaloacetate


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)


substrate_name: IMP

substrate: IMP


substrate_name: p-nitrophenyl phosphate (at pH 5.4 and 37 degrees Celsius)


organism: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

substrate_name: pNPP (at 76 degrees Celsius)

substrate: pNPP (at 76 degrees Celsius)


enzyme_display_name: alpha-amylase

organism: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

substrate_name: maltose (at pH 6.8 and 37 degrees Celsius)

enzyme: alpha-amylase


enzyme_display_name: alpha-glucosidase

organism: Erwinia rhapontici

substrate_name: maltose

enzyme: alpha-glucosidase

substrate: maltose


organism: Homo sapiens

substrate_name: maltoheptaose (with ctMGAM)

substrate: maltoheptaose (with ctMGAM)


substrate_name: p-nitrophenyl-alpha-D-glucopyranoside

substrate: p-nitrophenyl-alpha-D-glucopyranoside


enzyme_display_name: alpha-galactosidase

organism: Bacillus subtilis (strain 168)

substrate_name: melibiose

enzyme: alpha-galactosidase

substrate: melibiose


organism: Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019 / LMG 10263 / NCTC 9343 / Onslow / VPI 2553 / EN-2)

substrate_name: Galalpha-pNP

substrate: Galalpha-pNP


organism: Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)


organism: Aspergillus oryzae (strain ATCC 42149 / RIB 40)

substrate_name: birch wood xylan

substrate: birch wood xylan


assay_conditions_summary: pH 4.6; 310.15 K; buffer 0.1 M sodium citrate

proof_lines: mutant enzyme A20P, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


proof_lines: mutant enzyme E66Q, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


proof_lines: mutant enzyme N215S, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


proof_lines: mutant enzyme A97V, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


enzyme_display_name: versatile peroxidase

organism: Amycolatopsis sp.

substrate_name: H2O2; Reactive black 5

enzyme: versatile peroxidase

substrate: H2O2; Reactive black 5


organism: Phanerochaete chrysosporium

substrate_name: H2O2; 2,6-Dimethoxyphenol

substrate: H2O2; 2,6-Dimethoxyphenol


organism: Pleurotus eryngii


substrate_name: Reactive black 5

substrate: Reactive black 5


proof_lines:
pH 3.5, 25°C
native recombinant enzyme
pH 5.0, 25°C


organism: Rhodococcus jostii

smiles: OO


enzyme_display_name: dye decolorizing peroxidase

substrate_name: H2O2; Reactive blue 5

proof_lines: pH and temperature not specified in the publication

enzyme: dye decolorizing peroxidase

substrate: H2O2; Reactive blue 5


organism: Auricularia auricula-judae


organism: Methanosarcina thermophila

substrate_name: ATP

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

substrate: ATP


substrate_name: veratrylglycerol-beta-guaiacyl ether

proof_lines:
at pH 3.0 and 25°C
Raw SKiD Env: Temp=25°C, pH=3
Raw SKiD Env: Temp=25°C, pH=3.5

substrate: veratrylglycerol-beta-guaiacyl ether


substrate_name: Reactive Blue 19

substrate: Reactive Blue 19


organism: Dictyostelium discoideum

substrate_name: pyrogallol

substrate: pyrogallol


substrate_name: Reactive Blue 4

substrate: Reactive Blue 4


organism: Irpex lacteus


assay_conditions_summary: pH 7; 310.15 K; cofactor ATP

proof_lines: pH 7.0, 37°C, mutant H180D, produced in E. coli


substrate_name: acetate

proof_lines: pH 7.0, 37°C, mutant H90A, produced in E. coli

substrate: acetate


proof_lines: pH 7.0, 37°C, mutant H180K, produced in E. coli


proof_lines: pH 7.0, 37°C, mutant H180E, produced in E. coli


substrate_name: manganese

substrate: manganese


substrate_name: 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) (Dye-decolorizing peroxidase AauDyP1, at pH 4.5)

assay_conditions_summary: pH 3


organism: Exidia glandulosa


organism: Mycena epipterygia


organism: Mycetinis scorodonius


enzyme_display_name: catalase peroxidase

organism: Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)

substrate_name: H(2)O(2) for the catalase reaction (at pH 5.5-6.0)

enzyme: catalase peroxidase


organism: Burkholderia pseudomallei (strain K96243)

substrate: H(2)O(2) for the catalase reaction (at pH 5.5-6.0)


organism: Escherichia coli (strain K12)


organism: Geobacillus stearothermophilus


organism: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

substrate_name: H(2)O(2) for the catalase reaction

assay_conditions_summary: cofactor NADPH

substrate: H(2)O(2) for the catalase reaction


organism: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)


organism: Homo sapiens

proof_lines: mutant enzyme M296V, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


proof_lines: mutant enzyme A156V, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


proof_lines: mutant enzyme M72V, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


proof_lines: mutant enzyme L166V, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C