organism: Homo sapiens

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

assay_conditions_summary: pH 8.98; buffer for carbonic anhydrase I mutant entries

proof_lines: pH 8.98, TAPS buffer, carbonic anhydrase I mutant V62N and carbonic anhydrase I mutant H67N

proof_lines: pH 8.88, TAPS buffer, carbonic anhydrase I mutant V62N,H67N

proof_lines: pH 8.72, 1,2-dimethyimidazole buffer, carbonic anhydrase I mutant V62N,H67N


enzyme_display_name: beta-lactamase

organism: Psychrobacter immobilis A5

substrate_name: benzylpenicillin

source_db: brenda

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

protein_accession_database: UniProt

source_license: CC BY 4.0

km_unit: mM

sequence_source: UniProtKB/Swiss-Prot O05465 and NCBI Protein CAA58569.1, linked to PMID 9063463 and EMBL X83586.1

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

enzyme: beta-lactamase

substrate: benzylpenicillin

source: brenda


enzyme_display_name: beta-glucosidase

organism: Rhizomucor miehei

substrate_name: laminaribiose

sequence_source: UniProtKB direct REST check for A0A0B4RUW2 on 2026-06-25

enzyme: beta-glucosidase

substrate: laminaribiose


organism: Saccharolobus solfataricus

sequence_source: UniProtKB direct REST check for Q97UH8 on 2026-06-25


organism: Spodoptera frugiperda

substrate_name: 4-nitrophenyl beta-D-fucoside

sequence_variant_note: Resolved canonical sequence from UniProtKB direct REST check for O61594 on 2026-06-25 and applied mutation signature E194A to reconstruct the assayed variant sequence.

mutation_type: substitution

substrate: 4-nitrophenyl beta-D-fucoside


substrate_name: 4-nitrophenyl beta-D-glucoside

sequence_variant_note: Resolved canonical sequence from UniProtKB direct REST check for O61594 on 2026-06-25 and applied mutation signature E190Q to reconstruct the assayed variant sequence.

substrate: 4-nitrophenyl beta-D-glucoside


sequence_variant_note: Resolved canonical sequence from UniProtKB direct REST check for O61594 on 2026-06-25 and applied mutation signature K201A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB direct REST check for O61594 on 2026-06-25 and applied mutation signature E190A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB direct REST check for O61594 on 2026-06-25 and applied mutation signature K201F to reconstruct the assayed variant sequence.


sequence_source: UniProtKB direct REST check for O61594 on 2026-06-25


sequence_variant_note: Resolved canonical sequence from UniProtKB direct REST check for O61594 on 2026-06-25 and applied mutation signature M453A to reconstruct the assayed variant sequence.


organism: Talaromyces purpureogenus

kcat_display: Not reported

sequence_source: UniProtKB direct REST check for C9E9M9 on 2026-06-25

assay_conditions_summary: pH 5; 338.15 K; buffer 100 mM sodium acetate

proof_lines: in 100 mM sodium acetate buffer, pH 5.0, at 65°C


organism: Thermoascus aurantiacus

sequence_source: UniProtKB direct REST check for Q4U4W7 on 2026-06-25


substrate_name: 4-nitrophenyl beta-D-glucopyranoside

sequence_source: UniProtKB direct REST check for Q0ZUL0 on 2026-06-25

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Thermococcus kodakarensis

substrate_name: 4-nitrophenyl beta-D-mannopyranoside

proof_lines: 80°C, pH is not specified in the publication, mutant enzyme D206N

substrate: 4-nitrophenyl beta-D-mannopyranoside


sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: 80°C, pH is not specified in the publication, wild-type


organism: Thermofilum pendens

sequence_source: UniProtKB direct REST check for A1S0B1 on 2026-06-25


organism: Thermoplasma acidophilum

sequence_source: UniProtKB direct REST check for Q9HIL6 on 2026-06-25

assay_conditions_summary: pH 6; 363.15 K; buffer 100 mM citrate/phosphate

proof_lines: in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C


organism: Thermosphaera aggregans

substrate_name: 2-nitrophenyl beta-D-glucopyranoside

sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9YGA8 direct FASTA lookup, 2026-06-25 and applied mutation signature N62Y/Q63K/L231F/V303A/E386G/A412V to reconstruct the assayed variant sequence.

substrate: 2-nitrophenyl beta-D-glucopyranoside


sequence_source: UniProtKB:Q9YGA8 direct FASTA lookup, 2026-06-25

proof_lines: wild-type, pH 6.5, 65°C, presence of 2 M formate


sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

proof_lines: mutant E387G, pH 6.5, 65°C, presence of 2 M formate


organism: Thermothelomyces thermophilus

sequence_source: UniProtKB:G2QDN2 direct FASTA lookup, 2026-06-25


organism: Thermotoga neapolitana

sequence_variant_note: Resolved canonical sequence from UniProtKB:Q0GC07 direct FASTA lookup, 2026-06-25 and applied mutation signature W243F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q0GC07 direct FASTA lookup, 2026-06-25 and applied mutation signature D58A to reconstruct the assayed variant sequence.

proof_lines:
mutant D58A, pH 5.6, 90°C, 0.1 M NaN3
mutant D58A, pH 5.6, 90°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q0GC07 direct FASTA lookup, 2026-06-25 and applied mutation signature D242G to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:Q0GC07 direct FASTA lookup, 2026-06-25

proof_lines:
wild-type, pH 5.6, 90°C, 0.1 M NaN3
wild-type, pH 5.6, 90°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q0GC07 direct FASTA lookup, 2026-06-25 and applied mutation signature E458G to reconstruct the assayed variant sequence.

proof_lines:
mutant E458G, pH 5.6, 90°C
mutant E458G, pH 5.6, 90°C, 0.1 M NaN3


substrate_name: quercetin 3-O-beta-D-glucopyranoside

sequence_variant_note: Resolved canonical sequence from UniProtKB:B9K7M5 direct FASTA lookup, 2026-06-25 and applied mutation signature N220S to reconstruct the assayed variant sequence.

substrate: quercetin 3-O-beta-D-glucopyranoside


sequence_variant_note: Resolved canonical sequence from UniProtKB:B9K7M5 direct FASTA lookup, 2026-06-25 and applied mutation signature F310L to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B9K7M5 direct FASTA lookup, 2026-06-25 and applied mutation signature F224I to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B9K7M5 direct FASTA lookup, 2026-06-25 and applied mutation signature N221F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B9K7M5 direct FASTA lookup, 2026-06-25 and applied mutation signature F310E to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:B9K7M5 direct FASTA lookup, 2026-06-25

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B9K7M5 direct FASTA lookup, 2026-06-25 and applied mutation signature W322A to reconstruct the assayed variant sequence.


organism: Phanerodontia chrysosporium

substrate_name: cellobiose

sequence_source: UniProtKB:Q8TGC6 direct lookup 2026-06-25

substrate: cellobiose


substrate_name: p-nitrophenyl-beta-D-glucose

substrate: p-nitrophenyl-beta-D-glucose


sequence_source: UniProtKB:Q25BW5 direct lookup 2026-06-25

proof_lines: pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A


sequence_source: UniProtKB:Q25BW4 direct lookup 2026-06-25


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB:Q25BW5 direct lookup 2026-06-25 and applied mutation signature M177L to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB:Q25BW5 direct lookup 2026-06-25 and applied mutation signature K253A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB:Q25BW5 direct lookup 2026-06-25 and applied mutation signature D229N to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB:Q25BW5 direct lookup 2026-06-25 and applied mutation signature V173C to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB:Q25BW5 direct lookup 2026-06-25 and applied mutation signature H231D to reconstruct the assayed variant sequence.


organism: Phytophthora infestans

sequence_source: UniProtKB:Q9AT27 direct lookup 2026-06-25


organism: Piromyces sp.

substrate_name: p-nitrophenyl-beta-D-glucopyranoside

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

substrate: p-nitrophenyl-beta-D-glucopyranoside


organism: Putranjiva roxburghii

sequence_source: UniProtKB:A0A023MIF8 direct lookup 2026-06-25

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Pyrococcus furiosus

sequence_source: UniProtKB:Q51723 direct lookup 2026-06-25


sequence_source: UniProtKB:E7FHY4 direct lookup 2026-06-25


organism: Rasamsonia emersonii

sequence_source: UniProtKB:Q8TGI8 direct lookup 2026-06-25

proof_lines:
pH 5.0, 37°C
pH 5.0, 72°C, recombinant enzyme
pH 5.0, 72°C


organism: Halothermothrix orenii

sequence_variant_note: Resolved canonical sequence from UniProtKB:B8CYA8 canonical sequence with row mutation Y296F reconstructed on 2026-06-25 and applied mutation signature Y296F to reconstruct the assayed variant sequence.


verification_status: corrected

sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:B8CYA8 and applied mutation signature F417S to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 7; 343.15 K; buffer the coupled glucose oxidase/peroxidase method in 20 mM HEPES

column_label: Table 2 (Kinetic parameters for beta-glucosidase and beta-galactosidase activities). Column 1 is the enzyme form; columns 2-4 are the D-cellobiose block with heads 'k_cat,app (s^-1)', 'K_m (mM)', 'k_cat,app/K_m (mM^-1 s^-1)'; columns 5-7 are the D-lactose block with heads 'k_cat,app (s^-1)', 'K_m (mM)', 'k_cat,app/K_m (s^-1 mM^-1)'; column 8 is the selectivity ratio. Body row 'F417S', D-cellobiose block: k_cat,app = 0.38 ± 0.01; K_m = 11.9 ± 0.9; k_cat,app/K_m = 0.032.

normalized_value_unit_evidence: k_cat: printed Table 2 cell 0.38 ± 0.01 in column 'k_cat,app (s^-1)' of the D-cellobiose block; the value is already s^-1, so no conversion is applied and the paper value is 0.38 s^(-1). K_m: printed Table 2 cell 11.9 ± 0.9 in column 'K_m (mM)' of the D-cellobiose block; already mM, no conversion, paper value 11.9 mM. k_cat/K_m: printed Table 2 cell 0.032 in column 'k_cat,app/K_m (mM^-1 s^-1)'; mM^-1 s^-1 and s^-1 mM^-1 are the same unit, already the export unit, so no conversion and the paper value is 0.032 s^(-1)*mM^(-1). Closure check: 0.38 / 11.9 = 0.0319328 s^-1 mM^-1, which rounds to the printed efficiency cell 0.032 at the precision the table prints, so the printed ratio and the quotient of the printed components agree.

raw_value_unit_evidence: Table 2: Kinetic parameters for β-glucosidase and β-galactosidase activities (Applied microbiology and biotechnology 2016, 100(8):3533-3543), body row 'F417S', D-cellobiose block: column 'k_cat,app (s^-1)' prints 0.38 ± 0.01; column 'K_m (mM)' prints 11.9 ± 0.9; column 'k_cat,app/K_m (mM^-1 s^-1)' prints 0.032.

row_label: Table 2, body row 'F417S' (variant F417S), D-cellobiose block

status: corrected


substrate_name: lactose

sequence_variant_note: Resolved canonical sequence from UniProtKB:B8CYA8 canonical sequence with row mutation N294T reconstructed on 2026-06-25 and applied mutation signature N294T to reconstruct the assayed variant sequence.

substrate: lactose


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:B8CYA8 and applied mutation signature N222F to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic parameters for beta-glucosidase and beta-galactosidase activities). Column 1 is the enzyme form; columns 2-4 are the D-cellobiose block with heads 'k_cat,app (s^-1)', 'K_m (mM)', 'k_cat,app/K_m (mM^-1 s^-1)'; columns 5-7 are the D-lactose block with heads 'k_cat,app (s^-1)', 'K_m (mM)', 'k_cat,app/K_m (s^-1 mM^-1)'; column 8 is the selectivity ratio. Body row 'N222F', D-cellobiose block: k_cat,app = 16.9 ± 1.3; K_m = 93.2 ± 12.6; k_cat,app/K_m = 0.18.

normalized_value_unit_evidence: k_cat: printed Table 2 cell 16.9 ± 1.3 in column 'k_cat,app (s^-1)' of the D-cellobiose block; the value is already s^-1, so no conversion is applied and the paper value is 16.9 s^(-1). K_m: printed Table 2 cell 93.2 ± 12.6 in column 'K_m (mM)' of the D-cellobiose block; already mM, no conversion, paper value 93.2 mM. k_cat/K_m: printed Table 2 cell 0.18 in column 'k_cat,app/K_m (mM^-1 s^-1)'; mM^-1 s^-1 and s^-1 mM^-1 are the same unit, already the export unit, so no conversion and the paper value is 0.18 s^(-1)*mM^(-1). Closure check: 16.9 / 93.2 = 0.18133 s^-1 mM^-1, which rounds to the printed efficiency cell 0.18 at the precision the table prints, so the printed ratio and the quotient of the printed components agree.

raw_value_unit_evidence: Table 2: Kinetic parameters for β-glucosidase and β-galactosidase activities (Applied microbiology and biotechnology 2016, 100(8):3533-3543), body row 'N222F', D-cellobiose block: column 'k_cat,app (s^-1)' prints 16.9 ± 1.3; column 'K_m (mM)' prints 93.2 ± 12.6; column 'k_cat,app/K_m (mM^-1 s^-1)' prints 0.18.

row_label: Table 2, body row 'N222F' (variant N222F), D-cellobiose block


sequence_source: UniProtKB:B8CYA8 direct REST check on 2026-06-25


organism: Homo sapiens

sequence_source: UniProtKB:Q9H227 direct REST check on 2026-06-25


organism: Lamium galeobdolon

substrate_name: oleuropein

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

substrate: oleuropein


organism: Listeria innocua Clip11262

substrate_name: sophorotetraose

sequence_source: UniProtKB:Q92AS9 direct REST check on 2026-06-25

substrate: sophorotetraose


substrate_name: sophorose

sequence_variant_note: Resolved canonical sequence from UniProtKB:Q92AS9 canonical sequence with row mutation R572K reconstructed on 2026-06-25 and applied mutation signature R572K to reconstruct the assayed variant sequence.

substrate: sophorose


organism: Mycothermus thermophilus

sequence_source: UniProtKB:A0A191KCR1 direct REST check on 2026-06-25

proof_lines: pH 5.5, 60°C, with 4-nitrophenyl beta-D-glucopyranoside as substrate, wild-type enzyme


sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.


proof_lines: pH 6.0, 50°C, mutant enzyme I48W/I278F/T484Y


sequence_source: UniProtKB:A0A191KCR2 direct REST check on 2026-06-25

proof_lines: pH 6.0, 50°C, with 4-nitrophenyl beta-D-glucopyranoside as substrat, wild-type enzyme


sequence_source: UniProtKB A0A191KCR0 canonical sequence retrieved by direct accession lookup on 2026-06-25.

proof_lines: pH 5.5, 55°C, with 4-nitrophenyl beta-D-glucopyranoside as substrate


sequence_source: UniProtKB O93784 canonical sequence; source accession A0A076JRL8 is inactive/merged and redirects to O93784 in UniProtKB release 2026_02.


organism: Neotermes koshunensis

sequence_source: UniProtKB Q8T0W7 canonical sequence retrieved by direct accession lookup on 2026-06-25.

proof_lines: pH 5.5, 37°C, recombinant His-tagged enzyme


organism: Neurospora crassa

sequence_source: UniProtKB Q7S3R5 canonical sequence retrieved by direct accession lookup on 2026-06-25.


organism: Oryza sativa

substrate_name: cellotriose

sequence_source: UniProtKB Q75I93 canonical sequence retrieved by direct accession lookup on 2026-06-25.

substrate: cellotriose


sequence_source: UniProtKB Q75I93 canonical sequence; row-specific mutant sequence not accepted without complete mutation-coordinate and kinetic record evidence.


substrate_name: gentiobiose

sequence_source: UniProtKB Q8L7J2 canonical sequence retrieved by direct accession lookup on 2026-06-25.

proof_lines: beta-(1-6)-linked gentiobiose, in 100 mM sodium acetate, pH 5.0, at 30°C

proof_lines: beta-(1-2)-linked sophorose, in 100 mM sodium acetate, pH 5.0, at 30°C

substrate: gentiobiose


sequence_source: UniProtKB Q10CU9 canonical sequence retrieved by direct accession lookup on 2026-06-25.

proof_lines: pH and temperature not specified in the publication


organism: Paenibacillus polymyxa

sequence_source: UniProtKB P22073 canonical sequence retrieved by direct accession lookup on 2026-06-25.


organism: Paenibacillus sp.

sequence_source: UniProtKB Q2WGB4 canonical sequence retrieved by direct accession lookup on 2026-06-25.


organism: Pectobacterium carotovorum

sequence_source: UniProtKB Q6QGY5 canonical sequence retrieved by direct accession lookup on 2026-06-25.


organism: Penicillium brasilianum

sequence_source: UniProtKB A5A4M8 canonical sequence retrieved by direct accession lookup on 2026-06-25.

proof_lines: recombinant enzyme, in 50 mM sodium citrate buffer pH 4.8, at 22°C

proof_lines: recombinant enzyme, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C

proof_lines: recombinant enzyme, using cellobiose as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C


organism: Periconia sp. BCC 2871

sequence_source: UniProtKB A9UIG0 canonical sequence retrieved by direct accession lookup on 2026-06-25.


organism: Gelatoporia subvermispora


organism: Unknown


organism: Aspergillus niger

substrate_name: Unknown

km_display: Not reported

ki_unit: mM

substrate: Unknown


proof_lines: pH 4.5, 25°C, recombinant mutants W49N and W49G


proof_lines: pH 4.5, 25°C, recombinant wild-type enzyme

proof_lines: wild type enzyme, in 0.1 M sodium acetate, pH 4.0, at 40°C

proof_lines: wild type enzyme, in 0.1 M sodium acetate, pH 6.0, at 40°C


proof_lines: mutant enzyme D496A, in 0.1 M sodium acetate, pH 4.0, at 40°C


proof_lines: mutant enzyme D487A, in 0.1 M sodium acetate, pH 4.0, at 40°C


proof_lines: mutant enzyme D501A, in 0.1 M sodium acetate, pH 4.0, at 40°C


substrate_name: 2,4-dinitrophenyl beta-D-glucoside

proof_lines: mutant enzyme E490A, in 0.1 M sodium acetate, pH 6.0, at 40°C

proof_lines: mutant enzyme E490A, in 0.1 M sodium acetate, pH 4.0, at 40°C

substrate: 2,4-dinitrophenyl beta-D-glucoside


proof_lines: mutant enzyme D463A, in 0.1 M sodium acetate, pH 4.0, at 40°C


assay_conditions_summary: pH 4.8; 323.15 K; buffer 50 mM citrate

proof_lines: in 50 mM citrate buffer, pH 4.8, at 50°C


organism: Aspergillus oryzae


organism: Thermotoga neapolitana

sequence_variant_note: Resolved canonical sequence from UniProtKB B9K7M5 canonical sequence with selected mutation N221S applied and applied mutation signature N221S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB B9K7M5 canonical sequence with selected mutation N220F applied and applied mutation signature N220F to reconstruct the assayed variant sequence.


organism: Thermotoga petrophila

sequence_source: UniProtKB A5IL97 canonical sequence from direct REST lookup

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


sequence_source: UniProtKB D2C716 canonical sequence from direct REST lookup


sequence_source: UniProtKB A5IL43 canonical sequence from direct REST lookup

proof_lines:
pH 5.0, 90°C, without Zn2+
pH 5.0, 90°C, 2 mM Zn2+


organism: Thermus thermophilus


organism: Trichoderma reesei

sequence_source: UniProtKB Q7Z9M5 canonical sequence from direct REST lookup


organism: Trichoderma viride

sequence_source: UniProtKB C6GGC9 canonical sequence only; variant sequence not reconstructed

proof_lines: mutant enzyme Q627C/R543H/R646V, at pH 5.0 and 50°C


sequence_source: UniProtKB C6GGC9 canonical sequence from direct REST lookup

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


organism: uncultured bacterium


organism: uncultured microorganism

substrate_name: 4-nitrophenyl beta-D-fucopyranoside

sequence_source: UniProtKB A0A1E1FFN6 canonical sequence only; variant sequence not reconstructed

proof_lines: mutant N223Q, presence of 500 mM D-glucose, pH 5.5, 75°C

substrate: 4-nitrophenyl beta-D-fucopyranoside


sequence_source: UniProtKB A0A1E1FFN6 canonical sequence from direct REST lookup

proof_lines: wild-type, presence of 500 mM D-glucose, pH 5.5, 75°C


proof_lines: mutant N223Y, presence of 500 mM D-glucose, pH 5.5, 75°C


proof_lines: pH 6.0, 60°C, additive: 25 mM cellobiose

proof_lines: pH 6.0, 60°C, additive: 250 mM D-galactose


organism: uncultured rumen bacterium

sequence_source: UniProtKB D3VYD8 canonical sequence from direct REST lookup

proof_lines: pH 6.0, 50°C, compared to KM-value: 0.03 mM for 4-nitrophenyl-beta-D-xylopyranoside of the beta-xylosidase activity, suggesting that RuBGX1 prefers 4-nitrophenyl-beta-D-xylopyranoside substrate


organism: Volvariella volvacea

substrate_name: 4-nitrophenyl beta-D-glucoside

sequence_source: UniProtKB:Q9C3Z9 REST lookup, sequenceVersion 1

substrate: 4-nitrophenyl beta-D-glucoside


organism: Weissella cibaria

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

sequence_source: UniProtKB:I6TNE2 REST lookup, sequenceVersion 1

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Zea mays

sequence_source: UniProtKB:P49235 REST lookup, sequenceVersion 1

sequence_variant_note: Mutation signature F198V could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature D261N matches the mutant residue at offset +15 and the reference residue at offset +8; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


substrate_name: Dhurrin

sequence_source: UniProtKB:P49235 canonical sequence checked; row-specific variant sequence unresolved

proof_lines: pH 5.8, 37°C, chimeric mutant (Chim16) bearing a C-terminal octapeptide (466-477) exchanged with the homologous octapeptide of Sorghum Dhr1 (with an extra 22 amino acids substitution)

proof_lines: pH 5.8, 37°C, chimeric mutant (Chim21) bearing a C-terminal octapeptide (466-477) exchanged with the homologous octapeptide of Sorghum Dhr1

substrate: Dhurrin


sequence_variant_note: Mutation signature Y473F could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature F205L could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature M263F could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature P377A matches the mutant residue at offset -1 and the reference residue at offset -12; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_variant_note: Mutation signature F466S/A467S could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature F466S could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature F198V/F205L/P377A could not be applied safely because the resolved sequence does not match the expected reference residue(s).


proof_lines:
wild-type
pH 5.8, 37°C, wild-type


sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset +12, so it is treated as a source-provided variant sequence.


sequence_variant_note: Mutation signature W373K could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature F193A matches the mutant residue at offset +9 and the reference residue at offset +2; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


organism: Aspergillus saccharolyticus

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: pH and temperature not specified in the publication


organism: Aspergillus terreus


organism: Aspergillus tubingensis


organism: Aspergillus versicolor

proof_lines: free enzyme, at 37°C, pH not specified in the publication

proof_lines: immobilized enzyme, at 37°C, pH not specified in the publication


organism: Aspergillus wentii


organism: Avena sativa


proof_lines:
pH 6.8, isoform AsGlu2
pH 6.8, isoform AsGlu1
pH 6.8, 30°C, hexameric enzyme form


organism: Bacillus sp. ZJ1308


organism: Bacillus subtilis

assay_conditions_summary: pH 5

proof_lines: pH 5.0, temperature not specified in the publication


organism: Badhamia polycephala


organism: Bifidobacterium breve


organism: Bos taurus


organism: Acetivibrio thermocellus

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


organism: Agroathelia rolfsii

proof_lines:
beta-glucosidase 3
beta-glucosidase 1
beta-glucosidase 2


organism: Agrobacterium tumefaciens

proof_lines:
no change for chimeric enzymes
marked increase for chimeric enzyme


organism: Alternaria alternata

substrate_name: cellobiose; 4-nitrophenyl-alpha-D-glucoside; Salicin; Laminarin; 4-nitrophenyl-beta-D-galactoside


organism: Apis mellifera

proof_lines: 35°C, pH 5.0, beta-glucosidase purified from ventriculus

proof_lines: 35°C, pH 5.0, beta-glucosidase purified from honey sac


organism: Aspergillus aculeatus


organism: Aspergillus fischeri


organism: Aspergillus foetidus


organism: Aspergillus fumigatus

substrate_name: 4-nitrophenyl-beta-D-glucopyranoside; cellobiose; gentiobiose; 4-nitrophenyl beta-D-glucopyranoside


organism: Aspergillus japonicus

substrate_name: cellobiose; 4-nitrophenyl-beta-D-glucopyranoside; p-nitrophenyl-beta-D-glucopyranoside; amygdalin; Salicin; 2-Nitrophenyl-beta-D-glucopyranoside; methyl-beta-D-glucoside

proof_lines:
at pH 5.0 and 40°C
30°C, 5.0


organism: Aspergillus niger


organism: Zea mays

sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235; reconstructed F200K variant using PMID 19016858 active-site numbering mapped to the UniProt precursor with offset +59 and applied mutation signature F200K with inferred residue-numbering offset +59 to reconstruct the assayed variant sequence.


organism: Caldicellulosiruptor saccharolyticus

substrate_name: 2-nitrophenyl beta-D-glucopyranoside

substrate: 2-nitrophenyl beta-D-glucopyranoside


organism: [Candida] sake

substrate_name: p-nitrophenyl-beta-D-glucopyranoside


organism: Carica papaya


organism: Cellulomonas biazotea

substrate_name: cellobiose


organism: Cellulomonas flavigena


organism: Cellulomonas gilvus


assay_conditions_summary: pH 6.5; 303.15 K; buffer 50 mM MOPS

proof_lines: wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C


organism: Cerrena unicolor


organism: Chilo suppressalis

assay_conditions_summary: pH 5; 310.15 K; buffer 100 mM universal

proof_lines: enzyme from salivary gland, in 100 mM universal buffer (pH 5.0) at 37°C

proof_lines: enzyme from midgut, in 100 mM universal buffer (pH 5.0) at 37°C


organism: Citrus sinensis

substrate_name: p-nitrophenyl-beta-D-glucopyranose

substrate: p-nitrophenyl-beta-D-glucopyranose


organism: Dalbergia cochinchinensis

proof_lines:
pH 5.0, 30°C, native enzyme
pH 5.0, 30°C, recombinant enzyme


organism: Halalkalibacterium halodurans


organism: Hanseniaspora uvarum


organism: Hanseniaspora vineae

substrate_name: Salicin

substrate: Salicin


organism: Homo sapiens

proof_lines:
37°C, pH 6.5
50°C, pH 6.5
pH 7.0, recombinant enzyme


substrate_name: 4-methylumbelliferyl beta-D-glucoside

proof_lines: pH 5.5, 37°C, mutant N370S/N370S, Gauchers disease patient

proof_lines: pH 5.5, 37°C, mutant N370S and deletion mutation, Gauchers disease patient

proof_lines: pH 5.5, 37°C, mutant N370S, heterozygote

substrate: 4-methylumbelliferyl beta-D-glucoside


proof_lines: pH 5.5, 37°C, mutant L444P, heterozygote


proof_lines: pH 5.5, 37°C, mutant N370S/L444P, Gauchers disease patient


proof_lines: pH 5.5, 37°C, mutant D409H, heterozygote


organism: Hordeum vulgare

substrate_name: sophorose


organism: Humicola sp.

proof_lines:
extracellular enzyme
intracellular enzyme


organism: Jeotgalibacillus malaysiensis


organism: Agrobacterium tumefaciens

proof_lines: mutant H229S, pH not specified in the publication, temperature not specified in the publication

substrate: cellobiose


proof_lines: mutant V176A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant L178A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant C174V, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant L178E, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant W127F, pH not specified in the publication, temperature not specified in the publication


proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication

proof_lines: wild-type, presence of 0.25 M glucose, pH not specified in the publication, temperature not specified in the publication

proof_lines: wild-type, presence of 0.5 M glucose, pH not specified in the publication, temperature not specified in the publication


organism: Anoxybacillus flavithermus subsp. yunnanensis


enzyme_display_name: Beta-glucosidase 14 (AtBGLU14) (EC 3.2.1.21)

organism: Arabidopsis thaliana

sequence_source: UniProtKB direct lookup accession Q9SLA0.

proof_lines: with ortho-nitrophenyl-beta-D-glucopyranoside as substrate

proof_lines: with 4-methylumbelliferyl-beta-D-glucopyranoside as substrate

proof_lines: with para-nitrophenyl-beta-D-glucopyranoside as substrate


enzyme_display_name: Beta-glucosidase 21 (AtBGLU21) (EC 3.2.1.21) (Protein PHOSPHATE STARVATION-RESPONSE 3.2)

substrate_name: scopolin

sequence_source: UniProtKB direct lookup accession Q9C525.

proof_lines:
recombinant enzyme, pH 6, 37°C
native enzyme, pH 6, 37°C

substrate: scopolin


enzyme_display_name: beta-glucosidase (EC 3.2.1.21)

organism: Aspergillus niger

sequence_source: UniProtKB direct lookup accession Q9P8F4.

enzyme: beta-glucosidase (EC 3.2.1.21)


organism: Caldicellulosiruptor saccharolyticus

sequence_source: UniProtKB direct lookup accession A4XIG7.

assay_conditions_summary: pH 5.5; 343.15 K; buffer 50 mM citrate

proof_lines: in 50 mM citrate buffer, at pH 5.5 and 70°C


enzyme_display_name: Ipecoside beta-D-glucosidase IpeGLU1 (EC 3.2.1.220) (Ipecac alkaloid beta-glucosidase 1) (IpeGLU1) (Strictosidine beta-glucosidase IpeGLU1) (EC 3.2.1.105)

organism: Carapichea ipecacuanha

substrate_name: strictosidine

substrate: strictosidine


organism: Coprinopsis cinerea

sequence_source: UniProtKB direct lookup accession A8NIX3.

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Glycine max

substrate_name: daidzein 7'-O-beta-D-glucoside

sequence_source: UniProtKB direct lookup accession Q08IT7.

substrate: daidzein 7'-O-beta-D-glucoside


enzyme_display_name: Beta-glucosidase (EC 3.2.1.21)

organism: Halothermothrix orenii

substrate_name: lactose

sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:B8CYA8 and applied mutation signature F417Y to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic parameters for beta-glucosidase and beta-galactosidase activities). Column 1 is the enzyme form; columns 2-4 are the D-cellobiose block with heads 'k_cat,app (s^-1)', 'K_m (mM)', 'k_cat,app/K_m (mM^-1 s^-1)'; columns 5-7 are the D-lactose block with heads 'k_cat,app (s^-1)', 'K_m (mM)', 'k_cat,app/K_m (s^-1 mM^-1)'; column 8 is the selectivity ratio. Body row 'F417Y', D-lactose block: k_cat,app = 41.5 ± 1.2; K_m = 10.3 ± 0.9; k_cat,app/K_m = 4.0.

normalized_value_unit_evidence: k_cat: printed Table 2 cell 41.5 ± 1.2 in column 'k_cat,app (s^-1)' of the D-lactose block; the value is already s^-1, so no conversion is applied and the paper value is 41.5 s^(-1). K_m: printed Table 2 cell 10.3 ± 0.9 in column 'K_m (mM)' of the D-lactose block; already mM, no conversion, paper value 10.3 mM. k_cat/K_m: printed Table 2 cell 4.0 in column 'k_cat,app/K_m (s^-1 mM^-1)'; mM^-1 s^-1 and s^-1 mM^-1 are the same unit, already the export unit, so no conversion and the paper value is 4.0 s^(-1)*mM^(-1). Closure check: 41.5 / 10.3 = 4.02913 s^-1 mM^-1, which rounds to the printed efficiency cell 4.0 at the precision the table prints, so the printed ratio and the quotient of the printed components agree.

raw_value_unit_evidence: Table 2: Kinetic parameters for β-glucosidase and β-galactosidase activities (Applied microbiology and biotechnology 2016, 100(8):3533-3543), body row 'F417Y', D-lactose block: column 'k_cat,app (s^-1)' prints 41.5 ± 1.2; column 'K_m (mM)' prints 10.3 ± 0.9; column 'k_cat,app/K_m (s^-1 mM^-1)' prints 4.0.

row_label: Table 2, body row 'F417Y' (variant F417Y), D-lactose block

enzyme: Beta-glucosidase (EC 3.2.1.21)

substrate: lactose


organism: Daldinia eschscholtzii

proof_lines: pH and temperature not specified in the publication


organism: Erinnyis ello


organism: Eubacterium sp.

substrate_name: esculin

substrate: esculin


organism: Evernia prunastri


organism: Flavobacterium sp.

substrate_name: Gentiotriose

substrate: Gentiotriose


organism: Fomitopsis palustris

proof_lines: isoform BGL1, pH not specified in the publication, at 55°C


organism: Fusarium oxysporum


organism: Fusarium proliferatum

substrate_name: ginsenoside Rg3

assay_conditions_summary: pH 5; 323.15 K; buffer note in NaAc/HAc

proof_lines: in NaAc/HAc buffer (0.2 M, pH 5.0), at 50°C

substrate: ginsenoside Rg3


organism: Fusarium solani

substrate_name: 4-nitrophenyl beta-D-glucopyranoside


organism: Fusobacterium sp.

substrate: p-nitrophenyl-beta-D-glucopyranoside


organism: Galleria mellonella

proof_lines: in 50 mM sodium acetate, pH 5.5, at 37°C


substrate_name: p-nitrophenyl-alpha-D-mannopyranoside

substrate: p-nitrophenyl-alpha-D-mannopyranoside


organism: Mucor circinelloides


organism: Mycothermus thermophilus

assay_conditions_summary: pH 5; 323.15 K; buffer 50 mM sodium phosphate

proof_lines: at 50°C, in 50 mM sodium phosphate buffer, pH 6.0


organism: Nakazawaea peltata


organism: Nakazawaea wickerhamii


organism: Olea europaea


organism: Orobanche minor


organism: Orpinomyces sp.


organism: Oryza sativa

substrate_name: tuberonic acid beta-D-glucoside

substrate: tuberonic acid beta-D-glucoside


organism: Paecilomyces sp.


organism: Paecilomyces sp. 'thermophila'

substrate: sophorose


organism: Paenibacillus polymyxa


organism: Penicillium aurantiogriseum

substrate_name: amygdalin

proof_lines:
for PGI
for PGII

substrate: amygdalin


organism: Sorghum bicolor

substrate_name: Dhurrin

substrate: Dhurrin


substrate_name: 2-nitrophenyl beta-D-glucopyranoside

substrate: 2-nitrophenyl beta-D-glucopyranoside


organism: Spodoptera frugiperda

substrate_name: p-nitrophenyl-beta-D-galactopyranoside

sequence_source: UniProt O61594 / NCBI AAC06038.1, linked from PMID 11342030.

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: vs. p-nitrophenyl-beta-D-glucopyranoside,30°C, pH 6.0

substrate: p-nitrophenyl-beta-D-galactopyranoside


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt O61594 / NCBI AAC06038.1 canonical Sfbetagly sequence using the selected PMID 20027479 mutation signature. and applied mutation signature Q39N/E451S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt O61594 / NCBI AAC06038.1 canonical Sfbetagly sequence using the selected PMID 20027479 mutation signature. and applied mutation signature Q39N to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt O61594 / NCBI AAC06038.1 canonical Sfbetagly sequence using the selected PMID 20027479 mutation signature. and applied mutation signature Q39E to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt O61594 / NCBI AAC06038.1 canonical Sfbetagly sequence using the selected PMID 20027479 mutation signature. and applied mutation signature E451A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt O61594 / NCBI AAC06038.1 canonical Sfbetagly sequence using the selected PMID 20027479 mutation signature. and applied mutation signature Q39E/E451S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt O61594 / NCBI AAC06038.1 canonical Sfbetagly sequence using the selected PMID 20027479 mutation signature. and applied mutation signature E451S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt O61594 / NCBI AAC06038.1 canonical Sfbetagly sequence using the selected PMID 20027479 mutation signature. and applied mutation signature Q39E/E451A to reconstruct the assayed variant sequence.


organism: Rhizobium leguminosarum


organism: Rhizomucor miehei


organism: Rhynchophorus palmarum

assay_conditions_summary: pH 5; 310.15 K; buffer 100 mM acetate

proof_lines: in 100 mM acetate buffer, pH 5.0, at 37°C


organism: Rhynchosciara americana


organism: Ruminococcus albus


organism: Saccharolobus solfataricus

substrate_name: daidzin

substrate: daidzin


organism: Saccharomyces cerevisiae


organism: Schwanniomyces etchellsii

substrate_name: p-nitrophenyl-beta-D-glucopyranoside


organism: Schwanniomyces vanrijiae

substrate_name: p-nitro-phenyl-beta-D-glucopyranoside

substrate: p-nitro-phenyl-beta-D-glucopyranoside


organism: Sclerocleista ornata


organism: Sclerotinia sclerotiorum


organism: Scytalidium lignicola


organism: Penicillium citrinum

substrate_name: cellobiose

substrate: cellobiose


organism: Penicillium decumbens


organism: Penicillium occitanis (nom. inval.)


organism: Pholiota adiposa


organism: Phoma sp.


organism: Plumeria obtusa

substrate_name: plumieride coumarate beta-D-glucoside

substrate: plumieride coumarate beta-D-glucoside


organism: Prunus armeniaca

proof_lines:
pH 6.0, 35°C
isoform A, pH 5.0, 50°C
isoform B, pH 5.0, 50°C


organism: Prunus dulcis

proof_lines: enzyme immobilized on polysulphone capillary membranes, at pH 6.5 and 25°C

proof_lines: increased Km if enzyme immobilized on cellulose


organism: Pseudomonas sp.

proof_lines: 40°C, pH 6.0, vs. genistein-7-O-beta-D-glucopyranoside


organism: Pyricularia oryzae

substrate_name: cellotetraose

substrate: cellotetraose


organism: Rasamsonia emersonii

substrate_name: Unknown

substrate: Unknown


organism: Rattus sp.


organism: Thermobifida fusca

substrate_name: D-cellobiose

sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature N439S to reconstruct the assayed variant sequence.

data_origin: experimental

substrate: D-cellobiose


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature L444F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature N317Y/L444F to reconstruct the assayed variant sequence.

proof_lines: pH 7.2, 25°C, recombinant mutant N317Y/L444F


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature N317Y/L444F/A433V to reconstruct the assayed variant sequence.

proof_lines: pH 7.2, 25°C, recombinant mutant N317Y/L444F/A433V


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature N178I to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature A433V to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature N317Y to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature N317Y/N439S to reconstruct the assayed variant sequence.

proof_lines: pH 7.2, 25°C, recombinant mutant N317Y/N439S


sequence_source: UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context

proof_lines: pH 7.2, 25°C, recombinant wild-type enzyme


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature S319C to reconstruct the assayed variant sequence.


organism: Thermochaetoides thermophila

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


organism: Thermoclostridium stercorarium


organism: Kluyveromyces marxianus

substrate_name: Salicin

substrate: Salicin


organism: Komagataella pastoris

substrate_name: 4-methylumbelliferyl beta-D-glucoside

proof_lines: pH 7.3, 40°C, versus substrate 4-nitrophenyl beta-D-glucopyranoside

substrate: 4-methylumbelliferyl beta-D-glucoside


organism: Lentinula edodes

substrate: p-nitrophenyl-beta-D-glucopyranoside


organism: Lenzites trabea


organism: Levilactobacillus brevis

assay_conditions_summary: pH 5.5; 310.15 K; buffer 0.2 mM citrate-phosphate

proof_lines: in 0.2 mM citrate-phosphate buffer, pH 5.5, at 37°C


organism: Linum usitatissimum

substrate_name: linustatin

substrate: linustatin


organism: Lissachatina fulica


organism: Lotus japonicus

substrate_name: rhodiocyanoside A

substrate: rhodiocyanoside A


organism: Manihot esculenta


organism: Melanocarpus sp.


organism: Metschnikowia pulcherrima

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Millerozyma farinosa


organism: Spodoptera frugiperda


organism: Sporotrichum pulverulentum

proof_lines:
for enzyme A
cell wall bound enzyme
for enzyme B


organism: Stachybotrys sp.

proof_lines:
pH 6.0, 50°C
pH 4.8, 50°C
at 50°C and pH 5.0


organism: Stereum hirsutum


organism: Streptomyces sp.


organism: Sus scrofa


organism: Talaromyces funiculosus

proof_lines: pH and temperature not specified in the publication


organism: Talaromyces verruculosus


organism: Termitomyces clypeatus

assay_conditions_summary: pH 5; 318.15 K; buffer 0.1 M NaOAc

proof_lines: purified enzyme, in 0.1 M NaOAc buffer, pH 5.0, at 45°C


organism: Termitomyces sp.


organism: Terrabacter ginsenosidimutans


organism: Thermoascus aurantiacus

proof_lines:
pH 5, 50°C
free enzyme, at pH 5.0 and 37°C
immobilized enzyme, at pH 5.0 and 37°C


organism: Zea mays


organism: Thermomyces dupontii

proof_lines: versus 2-nitrophenyl-beta-D-galactopyranoside, pH 6.5, 40°C


organism: Thermomyces lanuginosus


organism: thermophilic anaerobic bacterium


organism: Thermothelomyces heterothallicus

proof_lines: immobilized enzyme to polyacrylamide gel


organism: Thermothelomyces thermophilus

substrate_name: 4-nitrophenyl beta-D-glucose

proof_lines:
pH 5.4, 50°C
at pH 5.0 and 50°C
at pH 5.0 and 60°C

substrate: 4-nitrophenyl beta-D-glucose


organism: Thermotoga maritima

proof_lines: wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C


proof_lines: chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C

proof_lines: chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C


organism: Thermotoga petrophila


organism: Thermus sp.


organism: Thermus thermophilus


organism: Thielaviopsis paradoxa


organism: Tolypocladium cylindrosporum


organism: Trametes versicolor


organism: Trichocladium griseum


organism: Trichoderma koningii


organism: Trichoderma reesei

assay_conditions_summary: pH 5; 313.15 K; buffer 50 mM citrate

proof_lines:
at pH 5.0 and 40°C
beta-glucosidase II
beta-glucosidase I


organism: Triglochin maritima

substrate_name: triglochinin

proof_lines:
beta-glucosidase B, pH 5, 30°C
beta-glucosidase C, pH 5, 30°C

substrate: triglochinin


organism: Triticum aestivum


organism: unidentified microorganism

substrate_name: polydatin

substrate: polydatin


organism: Vanilla planifolia

substrate_name: glucovanillin

substrate: glucovanillin


organism: Verruciconidia persicina


organism: Wickerhamomyces anomalus


organism: Zea mays

substrate_name: 2-nitrophenyl beta-D-glucopyranoside

substrate: 2-nitrophenyl beta-D-glucopyranoside


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: pH 5.8, 25°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1)

proof_lines: pH 5.8, 37°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1)

proof_lines: pH 5.8, 25°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1)


enzyme_display_name: carbonic anhydrase

organism: Homo sapiens

assay_conditions_summary: pH 8.94; buffer row

proof_lines: pH 8.94, 1,2-dimethylimidazole buffer, carbonic anhydrase II mutant N62V

enzyme: carbonic anhydrase


proof_lines: pH 8.94, 1,2-dimethylimidazole buffer, carbonic anhydrase II mutant T200H


assay_conditions_summary: pH 6.28; buffer contexts and no measurement_key or exact kinetic lock

proof_lines: pH 6.28, MES buffer, carbonic anhydrase I mutant V62N,H67N,H200T

proof_lines: pH 8.98, TAPS buffer, carbonic anhydrase I mutant V62N,H67N,H200T

proof_lines: pH 7.23, 1-methylimidazolebuffer, carbonic anhydrase I mutant V62N,H67N,H200T


organism: Methanosarcina thermophila

assay_conditions_summary: pH 7.5; 298.15 K; buffer condition

proof_lines: pH 7.5, 25°C, variant E84A, 50 mM MOPS-Ac buffer

proof_lines: pH 7.5, 25°C, variant E84A, 50 mM MOPS buffer

proof_lines: pH 7.5, 25°C, variant E84A, 50 mM IMID buffer


organism: Populus tremula

assay_conditions_summary: pH 6.6; buffer and pH 8

proof_lines:
pH 6.6, MES buffer
pH 8.8, TAPS buffer


enzyme_display_name: alpha-amino-acid esterase

organism: Acetobacter pasteurianus

substrate_name: amoxicillin

source_license: CC BY 4.0; CC BY-NC-ND 4.0

sequence_source: UniProtKB REST FASTA Q8VRK8; fetched 2026-06-25

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines:
30°C, pH 6.2
Raw SKiD Env: Temp=30°C, pH=6.2
Raw SKiD Env: Temp=30°C, pH=7

source_databases_merged: brenda skid

enzyme: alpha-amino-acid esterase

substrate: amoxicillin


substrate_name: valacyclovir

source_db: oed

verification_status: unverified

sequence_source: UniProtKB REST FASTA Q86WA6; fetched 2026-06-25

substrate: valacyclovir

status: unverified

source: oed


organism: Xanthomonas campestris pv. campestris

sequence_variant_note: Resolved canonical sequence from UniProtKB REST FASTA B0RS62; fetched 2026-06-25 and applied mutation signature N186D/A275P/V622I/E143G to reconstruct the assayed variant sequence.


substrate_name: cefadroxil

sequence_source: UniProtKB REST FASTA B0RS62; fetched 2026-06-25

proof_lines: pH 7.0, 25°C, recombinant wild-type enzyme and mutants N186D/A275P/V622I/E143G and N186D/A275P/V622I/E143H

substrate: cefadroxil


sequence_variant_note: Resolved canonical sequence from UniProtKB REST FASTA B0RS62; fetched 2026-06-25 and applied mutation signature N186D/A275P/V622I/E143H to reconstruct the assayed variant sequence.


substrate_name: cefaloglycin

proof_lines:
pH 6.0, 30°C, recombinant enzyme
30°C, pH 7.0
hydrolysis, pH 6.2, 27-30°C

substrate: cefaloglycin


organism: Bacillus mycoides

substrate_name: phenylalanine methylester

substrate: phenylalanine methylester


proof_lines:
pH 7.4, 37°C, recombinant enzyme
pH 7.4, 37°C


organism: Paracidovorax avenae

substrate_name: D-4-hydroxyphenylglycine methyl ester

substrate: D-4-hydroxyphenylglycine methyl ester


organism: Pseudomonas melanogenum

substrate_name: cefradine

substrate: cefradine


organism: Rattus norvegicus


organism: Xanthomonas campestris

substrate_name: D-phenylglycine methyl ester

substrate: D-phenylglycine methyl ester


organism: Xanthomonas citri

substrate_name: D-phenylglycine amide

proof_lines: hydrolysis, pH 6.4, 25°C, recombinant enzyme

proof_lines: acyl transfer from D-alpha-phenylglycine methyl ester to 7-amino-3-deacetoxycephalosporanic acid

substrate: D-phenylglycine amide


organism: Xanthomonas sp.


enzyme_display_name: alpha-mannosidase

organism: Canavalia ensiformis

substrate_name: p-nitrophenyl-alpha-D-mannoside

source_db: uniprot

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

enzyme: alpha-mannosidase

substrate: p-nitrophenyl-alpha-D-mannoside

source: uniprot


substrate_name: alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-D-GlcNAc (at pH 4.0 and 37 degrees Celsius in the presence of 1mM CoCl2)


substrate_name: p-nitrophenyl-alpha-D-mannopyranoside (pNP-Man) (at 37 degrees Celsius and at pH 4.5)


organism: Lablab purpureus

substrate_name: p-nitrophenyl-alpha-D-mannopyranoside

substrate: p-nitrophenyl-alpha-D-mannopyranoside


organism: Trichoderma reesei


organism: Trypanosoma cruzi


organism: Yarrowia lipolytica


organism: Drosophila melanogaster

substrate_name: Unknown

sequence_source: UniProtKB:O97043 direct REST lookup 2026-06-25

assay_conditions_summary: pH 6

substrate: Unknown


organism: Mus musculus

sequence_source: UniProtKB:O09159 direct REST lookup 2026-06-25


organism: Picrophilus oshimae


organism: Saccharolobus solfataricus

substrate_name: 4-nitrophenyl alpha-D-mannoside

kcat_over_km_origin: calculated

sequence_source: UniProtKB:Q97UK5 direct REST lookup 2026-06-25

substrate: 4-nitrophenyl alpha-D-mannoside


proof_lines: pH 5.0, 70°C, enzyme in complex with Co2+

proof_lines: pH 5.0, 70°C, enzyme in complex with Mn2+


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q97UK5 direct REST lookup 2026-06-25 and applied mutation signature H533Q to reconstruct the assayed variant sequence.


proof_lines: pH 5.0, 70°C, 1 mM Cd2+, mutant enzyme H533Q

proof_lines: pH 5.0, 70°C, 0.1 mM Zn2+, mutant enzyme H533Q

proof_lines: pH 5.0, 70°C, 1 mM Zn2+, mutant enzyme H533Q


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q97UK5 direct REST lookup 2026-06-25 and applied mutation signature H533E to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q97UK5 direct REST lookup 2026-06-25 and applied mutation signature H228E to reconstruct the assayed variant sequence.

proof_lines: pH 5.0, 70°C, 1 mM Co2+, mutant enzyme H228E

proof_lines: pH 5.0, 70°C, 1 mM Cd2+, mutant enzyme H228E

proof_lines: pH 5.0, 70°C, 1 mM Zn2+, mutant enzyme H228E


proof_lines: pH 5.0, 70°C, 1 mM Co2+, mutant enzyme H533E

proof_lines: pH 5.0, 70°C, 0.1 mM Zn2+, mutant enzyme H533E. KM is too high (above 10 mM for 4-nitrophenyl alpha-D-mannoside or.0.1 mM for Mn2+) to allow for determination of kinetic parameters other than kcat/KM

proof_lines: pH 5.0, 70°C, 1 mM Mn2+, mutant enzyme H533E. KM is too high (above 10 mM for 4-nitrophenyl alpha-D-mannoside or.0.1 mM for Mn2+) to allow for determination of kinetic parameters other than kcat/KM


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q97UK5 direct REST lookup 2026-06-25 and applied mutation signature H228Q to reconstruct the assayed variant sequence.


proof_lines: pH 5.0, 70°C, 1 mM Co2+, mutant enzyme H228Q

proof_lines: pH 5.0, 70°C, 1 mM Zn2+, mutant enzyme H228Q

proof_lines: pH 5.0, 70°C, 1 mM Mn2+, mutant enzyme H228Q. KM is too high (above 10 mM for 4-nitrophenyl alpha-D-mannoside or.0.1 mM for Mn2+) to allow for determination of kinetic parameters other than kcat/KM


sequence_source: UniProtKB Q97UK5

proof_lines: pH 5.0, 70°C, 1 mM Co2+, wild-type enzyme

proof_lines: pH 5.0, 70°C, 1 mM Cd2+, wild-type enzyme

proof_lines: pH 5.0, 70°C, 1 mM Mn2+, wild-type enzyme


organism: Streptococcus pyogenes M1 GAS

sequence_source: UniProtKB Q99YP5


proof_lines:
pH 6.8, 37°C, recombinant enzyme
pH 6.8, 37°C


organism: Mycobacterium tuberculosis

proof_lines: pH not specified in the publication, at 37°C


organism: Oryctolagus cuniculus


organism: Oryza sativa


organism: Phaseolus vulgaris

proof_lines:
alpha-mannosidase I
alpha-mannosidase II


organism: Prunus dulcis


organism: Prunus serotina


organism: Rattus sp.

proof_lines: unbound isoform from cobalt-chelating Sepharose

proof_lines: Tris-eluted isoform from cobalt-chelating Sepharose


organism: Saccharomyces cerevisiae


organism: Solanum lycopersicum


organism: Streptococcus pneumoniae


organism: Sus scrofa


organism: Thermotoga maritima


organism: Aspergillus fischeri

mutation_signature: reaction stopped with Na2CO3

mutation_type: truncation

proof_lines: pH 6.5, 50°C, 15 min, reaction stopped with Na2CO3


proof_lines: low binding up to 40°C, increased binding at 50°C


organism: Aspergillus flavus


organism: Aspergillus niger


organism: Aspergillus oryzae


organism: Aspergillus phoenicis

substrate_name: Mannan

substrate: Mannan


organism: Aspergillus sp.


organism: Canavalia ensiformis

proof_lines:
fairly constant between pH 4.5-6.0
at pH 7.0
at pH 3.5


organism: Canavalia sp.


organism: Candida albicans

proof_lines:
enzyme II
enzyme I


organism: Capsicum annuum


organism: Clostridium perfringens


organism: Drosophila melanogaster

substrate_name: 2,4-dinitrophenyl alpha-D-mannoside

substrate: 2,4-dinitrophenyl alpha-D-mannoside


organism: Equus caballus


organism: Gallus gallus

proof_lines:
pH 7.0, 37°C, Co2+-treated enzyme
pH 7.0, 37°C, not treated with Co(II)


organism: Helianthus annuus

proof_lines:
isoforms I
isoforms II


organism: Homo sapiens

proof_lines:
pH 5.0, 37°C, HCEC cell layer
pH 5.0, 37°C, LNZ-308 cell layer
pH 5.0, 37°C, LN-18 cell layer


organism: Lablab purpureus

substrate_name: 4-nitrophenyl alpha-D-mannopyranoside

sequence_source: UniProtKB reviewed accession C0HJW7; evidence at protein level; cites PMID 23422784.

substrate: 4-nitrophenyl alpha-D-mannopyranoside


organism: Lupinus angustifolius

proof_lines:
alpha-mannosidase B
alpha-mannosidase A


organism: Macaca radiata


organism: Medicago sativa


enzyme_display_name: methyl-branched lipid omega-hydroxylase

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

proof_lines: kcat is 11.7 min(-1) for cholesterol as substrate. kcat is 1.5 min(-1) for cholest-4-en-3-one as substrate.

enzyme: methyl-branched lipid omega-hydroxylase


enzyme_display_name: o-aminophenol oxidase

organism: Streptomyces antibioticus

proof_lines:
free enzyme, at pH 4.5 and 25°C
enzyme on nanogel, at pH 4.5 and 25°C
Raw SKiD Env: Temp=25°C, pH=4.5

enzyme: o-aminophenol oxidase


organism: Bauhinia monandra

substrate_name: o-aminophenol

substrate: o-aminophenol


organism: Streptomyces griseus

sequence_source: UniProtKB:B1VTI5; reviewed; GriF/grixazone synthase/o-aminophenol oxidase; PubMed 16282322 evidence


organism: Trametes coccinea

substrate_name: FMN

assay_conditions_summary: cofactor FMN

substrate: FMN


organism: Streptomyces griseus subsp. griseus (strain JCM 4626 / CBS 651.72 / NBRC 13350 / KCC S-0626 / ISP 5235)

substrate_name: alpha-aminophenol

proof_lines: kcat is 20 sec(-1) with alpha-aminophenol as substrate. kcat is 14 sec(-1) with 3,4-AHBAL as substrate. kcat is 18 sec(-1) with 2-amino-4-methylphenol as substrate. kcat is 0.8 sec(-1) with 3,4-dihydroxybenzaldehyde as substrate. kcat is 12

substrate: alpha-aminophenol


organism: Mycobacterium tuberculosis

substrate_name: geranylgeraniol

sequence_source: UniProtKB:P9WPP3 direct REST lookup on 2026-06-25

proof_lines:
at pH 7.4 and 25°C
Raw SKiD Env: Temp=25°C, pH=7.4

substrate: geranylgeraniol


substrate_name: farnesol

substrate: farnesol


organism: Bacillus cereus

substrate_name: benzylpenicillin

source_db: skid

source_license: CC BY-NC-ND 4.0

sequence_source: UniProtKB reviewed accession P14488 (BLAB_BACCE) direct REST lookup during worker run

substrate: benzylpenicillin

source: skid


organism: Bacillus licheniformis

substrate_name: penicillin G

sequence_source: UniProtKB reviewed accession P00808 (BLAC_BACLI) direct REST lookup during worker run

proof_lines:
Raw SKiD Env: Temp=22°C, pH=7
Raw SKiD Env: Temp=-----, pH=-----

substrate: penicillin G


organism: Aeromonas hydrophila

substrate_name: Imipenem

sequence_source: UniProt REST direct check, sp|P26918|BLAB_AERHY, reviewed Swiss-Prot entry

proof_lines:
Raw SKiD Env: Temp=-----, pH=8
Raw SKiD Env: Temp=-----, pH=9
Raw SKiD Env: Temp=30°C, pH=6.5

substrate: Imipenem


organism: Escherichia coli

substrate_name: ceftazidime

sequence_source: UniProtKB:P00811 direct accession lookup, release 2026_02

proof_lines:
pH 6.5, 22°C
Raw SKiD Env: Temp=-----, pH=-----

substrate: ceftazidime


substrate_name: nitrocefin

sequence_source: UniProtKB:P13661 direct accession lookup, release 2026_02

proof_lines: pH 7.2, temperature not specified in the publication, recombinant enzyme

substrate: nitrocefin


organism: Pseudomonas aeruginosa

sequence_source: UniProtKB:P16897 direct REST check (https://rest.uniprot.org/uniprotkb/P16897.json)


organism: Serratia marcescens

sequence_source: UniProtKB:P52699 direct REST check (https://rest.uniprot.org/uniprotkb/P52699.json)

proof_lines:
pH 7.5, 25°C
at 30°C
Raw SKiD Env: Temp=25°C, pH=7.5


organism: Psychrobacter immobilis

substrate_name: ampicillin

assay_conditions_summary: pH 7; 303.15 K; buffer 50 mM sodium phosphate

proof_lines: at 30°C in 50mM sodium phosphate buffer (pH 7.0)

substrate: ampicillin


enzyme_display_name: caffeine synthase

organism: Coffea arabica

substrate_name: Theobromine

enzyme: caffeine synthase

substrate: Theobromine


organism: Theobroma cacao


sequence_source: UniProtKB direct accession lookup Q8H0D3; BRENDA source row supplied the accession


substrate_name: S-adenosyl-L-methionine

sequence_source: UniProtKB direct accession lookup Q8H0D2; BRENDA source rows supplied the accession

substrate: S-adenosyl-L-methionine


organism: Paullinia cupana

sequence_source: UniProtKB direct accession lookup A0A061DEC0; BRENDA source row supplied the accession


organism: Camellia sinensis var. assamica

substrate_name: 1,3,7-trimethyluric acid

proof_lines: kcat is 0.00012 sec(-1) with 1,3,7-trimethyluric acid as substrate.

substrate: 1,3,7-trimethyluric acid


organism: Candida albicans

assay_conditions_summary: pH 8.3; 293.15 K; buffer 20 mM Tris

proof_lines: at 20°C, pH 8.3 in 20 mM Tris buffer and 20 mM NaClO4

proof_lines: CaNce103, at 25°C, pH 8.3 in 20 mM Tris buffer and 20 mM NaClO4, in the presence of 0.01 mM histamine


organism: Homo sapiens

assay_conditions_summary: pH 6.28; buffer conditions

proof_lines: pH 6.28, MES buffer, carbonic anhydrase I mutant V62N

proof_lines: pH 8.95, 1,2-dimethylimidazole buffer, carbonic anhydrase I mutant V62N

proof_lines: pH 8.98, TAPS buffer, carbonic anhydrase I mutant V62N


assay_conditions_summary: pH 8.94; buffer for carbonic anhydrase II mutant N67H

proof_lines: pH 8.94, 1,2-dimethylimidazole buffer, carbonic anhydrase II mutant N67H


organism: Methanosarcina thermophila

assay_conditions_summary: pH 7.5; 298.15 K; buffer 50 mM MOPS

proof_lines: pH 7.5, 25°C, variant E62D, 50 mM MOPS buffer


assay_conditions_summary: pH 7.5; 298.15 K; buffer conditions

proof_lines:
pH 7.5, 25°C, wild-type, D2O
pH 5.7-9.5, 25°C, wild-type
pH 6.5, 25°C, wild-type, D2O


proof_lines: pH 7.5, 25°C, variant E84C, 50 mM MOPS buffer


proof_lines: pH 7.5, 25°C, variant E62A/E84A, 50 mM MOPS buffer


proof_lines: pH 7.5, 25°C, variant E84C and E62A, 50 mM MOPS buffer


proof_lines: pH 7.5, 25°C, variant E62Y, 50 mM MOPS buffer


proof_lines: pH 7.5, 25°C, variant E84H, 50 mM MOPS buffer


organism: Oncorhynchus mykiss

substrate_name: 4-nitrophenyl acetate

assay_conditions_summary: pH 9; 298.15 K; buffer note

proof_lines: in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C

substrate: 4-nitrophenyl acetate


enzyme_display_name: 5-aminolevulinate synthase

organism: Unknown

substrate_name: succinyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: 5-aminolevulinate synthase

substrate: succinyl-CoA


substrate_name: glycine

assay_conditions_summary: pH 7.5; 293.15 K; cofactor CoA

proof_lines: co-substrate: succinyl-CoA, mutant Y428I/R433Q /G434N/E435T/L437N, pH 7.5, 20°C

substrate: glycine


proof_lines: co-substrate: succinyl-CoA, mutant A425G/Y428H/R433H/G434N/E435K/L437K, pH 7.5, 20°C


proof_lines: co-substrate: succinyl-CoA, mutant L437Q, pH 7.5, 20°C


proof_lines: co-substrate: succinyl-CoA, mutant R433K/G434K/E435Q/L437Q, pH 7.5, 20°C


organism: Gallus gallus


organism: Mus musculus

proof_lines:
pH 7.5, 20°C, wild-type
wild-type, pH 7.5, 30°C
pH 7.5, 30°C, wild-type


proof_lines:
pH 7.5, 20°C, mutant 2XALAS
pH 7.5, 30°C, mutant 2XALAS


assay_conditions_summary: pH 7.5; 303.15 K; cofactor CoA

proof_lines: mutant R85L, cosubstrate octanoyl-CoA, pH 7.5, 30°C

proof_lines: mutant R85L, cosubstrate succinyl-CoA, pH 7.5, 30°C


substrate_name: butanoyl-CoA

proof_lines: mutant R85K, cosubstrate succinyl-CoA, pH 7.5, 30°C

proof_lines: mutant R85K, cosubstrate 2-hydroxybutanoyl-CoA, pH 7.5, 30°C

substrate: butanoyl-CoA


proof_lines: mutant cosubstrate 2-hydroxybutanoyl-CoA, R85L/T430V, pH 7.5, 30°C

proof_lines: mutant cosubstrate succinyl-CoA, R85L/T430V, pH 7.5, 30°C


organism: Caulobacter vibrioides

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

assay_conditions_summary: pH 7.5; 298.15 K; cofactor CoA


proof_lines: mutant enzyme H340A/C398A, at pH 7.5 and 25°C


sequence_source: UniProtKB:Q9A8J8 direct accession lookup


organism: Cereibacter sphaeroides

sequence_source: UniProtKB:Q04512 direct accession lookup

proof_lines: isoform HemA, pH and temperature not specified in the publication


assay_conditions_summary: pH 7.4; 310.15 K; cofactor CoA

proof_lines: pH 7.4, 37°C, recombinant mutant DELTAAT


substrate_name: Unknown

sequence_source: UniProtKB:P22557 direct accession lookup

proof_lines: apparent value, pH and temperature not specified in the publication

substrate: Unknown


proof_lines: His-tagged mutant enzyme R511E, pH 7.4, 37°C


proof_lines: His-tagged wild-type enzyme, pH 7.4, 37°C

proof_lines: pH 7.4, 37°C, recombinant wild-type enzyme


assay_conditions_summary: pH 7.5; 291.15 K; cofactor CoA


sequence_source: UniProtKB:P08680 direct accession lookup

proof_lines:
wild-type enzyme, pH 7.5, 18°C
wild type enzyme, at pH 7.5 and 37°C
pH 7.5, 37°C, wild-type enzyme


assay_conditions_summary: pH 7.5; 310.15 K; cofactor CoA


assay_conditions_summary: pH 7.2; 310.15 K; cofactor CoA

proof_lines: mutant enzyme T83S/T363S, at pH 7.2 and 37°C


proof_lines: mutant C281P of isoform HemT, pH and temperature not specified in the publication


proof_lines: isoform HemT, pH and temperature not specified in the publication


organism: Escherichia coli

proof_lines: co-substrate: succinyl-CoA, mutant Y428N/P432N/R433I/G434E/E435K/L437K, pH 7.5, 20°C


proof_lines: co-substrate: succinyl-CoA, mutant N427H, pH 7.5, 20°C


proof_lines: co-substrate: succinyl-CoA, mutant V423L/Y428R/P432E/R433I/G434N/E435Q/L437K, pH 7.5, 20°C


proof_lines: co-substrate: succinyl-CoA, mutant A425T, pH 7.5, 20°C


proof_lines: co-substrate: succinyl-CoA, mutant V423I/A425P/Y428C/P432R/R433K/E435N, pH 7.5, 20°C


proof_lines: wild-type, co-substrate: succinyl-CoA, pH 7.5, 20°C


proof_lines:
pH 7.5, 30°C, ALAS(K313A mutant)/ALAS
pH 7.5, 30°C, ALAS/ALAS (K313A mutant)


proof_lines: recombinant erythroid isoform mutant G142C


proof_lines: recombinant erythroid isoform mutant G144S

proof_lines: recombinant erythroid isoform and mutant G144S


proof_lines: recombinant erythroid isoform mutant G144T


proof_lines: recombinant erythroid isoform mutant G144A


proof_lines: recombinant erythroid isoform mutant R433K


proof_lines: recombinant erythroid isoform mutant R439K


proof_lines: recombinant erythroid isoform mutant R433L


proof_lines:
recombinant erythroid isoform
pH 7.5, 30°C, ALAS/ALAS
pH 7.5, 30°C, ALAS


proof_lines: recombinant erythroid isoform mutant D279E


proof_lines: recombinant erythroid isoform mutant Y121F


organism: Rhodopseudomonas palustris

substrate_name: butyryl-CoA

substrate: butyryl-CoA


proof_lines: pH 7.5, 25°C, mutant enzyme R21P/T82S/N84L


proof_lines: pH 7.5, 25°C, mutant enzyme R21N/N84L/T362V


organism: Saccharomyces cerevisiae


organism: Serratia plymuthica


proof_lines: recombinant erythroid isoform mutant Y121H


organism: Paracoccus denitrificans


organism: Plasmodium falciparum


organism: Rattus norvegicus

proof_lines:
erythroid-specific isoform
papain untreated enzyme
papain treated enzyme


proof_lines: pH 7.5, 25°C, mutant enzyme R21N/T82A/T362V


proof_lines: pH 7.5, 25°C, mutant enzyme R21P/N84L/T362X


proof_lines: pH 7.5, 25°C, mutant enzyme R21P/T82S/N84L/T362V


proof_lines: mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C


sequence_source: UniProtKB:Q8I4X1 direct accession lookup

proof_lines: predicted, pH and temperature not specified in the publication. Important potential lead compounds which can serve as inhibitors of Plasmodium falciparum 5-aminolevulinate synthase are identified using pharmacophore modeling, virtual screen


organism: Rhodobacter capsulatus

sequence_source: UniProtKB:P18079 direct accession lookup


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProtKB:P18079 canonical sequence plus selected mutation signature I325M/V390Y/H391I and applied mutation signature I325M/V390Y/H391I to reconstruct the assayed variant sequence.

proof_lines: pH 7.5, 37°C, mutant enzyme I325M/V390Y/H391I


sequence_source: UniProtKB:Q6RVB3 direct accession lookup

assay_conditions_summary: pH 7.5; cofactor CoA


enzyme_display_name: protein farnesyltransferase

substrate_name: farnesyl diphosphate

proof_lines: in 50 mM Tris-HCl (pH 7.0) containing 10 mM MgCl2, 0.01 mM ZnCl2, and 20 nM enzyme, at 30°C

proof_lines: in 50 mM Tris-HCl (pH 7.0) containing 10 mM MgCl2, 0.01 mM ZnCl2, 20 nM enzyme, and 5 mM DTT, at 30°C

enzyme: protein farnesyltransferase

substrate: farnesyl diphosphate


organism: Aspergillus fumigatus

substrate_name: (2E,6E)-farnesyl diphosphate

substrate: (2E,6E)-farnesyl diphosphate


substrate_name: Thr-Lys-Cys-Val-Ile-Met

substrate: Thr-Lys-Cys-Val-Ile-Met


organism: Homo sapiens

substrate_name: HOM-GPP

substrate: HOM-GPP


substrate_name: dansyl-GCVKS

substrate: dansyl-GCVKS


substrate_name: dansyl-GCVDS

substrate: dansyl-GCVDS


substrate_name: YRASNRSCAIM

substrate: YRASNRSCAIM


proof_lines:
apparent turnover number
cosubstrate: dansyl-Gly-Cys-Val-Ile-Met
cosubstrate: farnesyl diphosphate


substrate_name: dansyl-GCQL

sequence_source: UniProtKB reviewed Swiss-Prot Q04631/FNTA_RAT direct accession lookup

proof_lines: with (2E,6E,10E)-3,7,11-trimethyl-12-(prop-2-yn-1-yloxy)dodeca-2,6,10-trien-1-yl diphosphate, pH and temperature not specified in the publication

proof_lines: with farnesyl diphosphate, pH and temperature not specified in the publication

substrate: dansyl-GCQL


enzyme_display_name: glycogen phosphorylase

organism: Dictyostelium discoideum

substrate_name: phosphate

proof_lines: isoenzyme b, in the presence of 3 mM 5-AMP

enzyme: glycogen phosphorylase

substrate: phosphate


organism: Locusta migratoria

substrate_name: glycogen

proof_lines:
phosphorylase a and ab
phosphorylase a
phosphorylase ab

substrate: glycogen


organism: Solanum tuberosum

substrate_name: alpha-D-Glucan

proof_lines:
fast isoenzyme, glycogen phosphorolysis
fast isoenzyme, starch phosphorolysis
fast isoenzyme, starch synthesis

substrate: alpha-D-Glucan


organism: Typha latifolia

substrate_name: maltoheptaose

proof_lines:
synthesis, isoenzyme P-2
synthesis, isoenzyme P-1

substrate: maltoheptaose


proof_lines: phosphorolysis synthesis


organism: Ipomoea batatas


organism: Lathyrus oleraceus

proof_lines:
phosphorolysis at pH 7.2
cytoplasmic phosphorylase
chloroplast phosphorylase


organism: Lethenteron camtschaticum

substrate_name: AMP

proof_lines:
phosphorylase b
phosphorylase a
phosphorylase b, pH 6.3

substrate: AMP


organism: Manduca sexta

proof_lines:
phosphorylase ab
phosphorylase a
phosphorylase b


organism: Mizuhopecten yessoensis

substrate_name: alpha-D-glucose 1-phosphate

substrate: alpha-D-glucose 1-phosphate


organism: Musa x paradisiaca

substrate_name: glucose 1-phosphate

proof_lines:
maltose synthesis, phosphorylase C
maltose synthesis, phosphorylase A
starch synthesis, phosphorylase A

substrate: glucose 1-phosphate


organism: Oryctolagus cuniculus

proof_lines:
phosphorylase a
phosphorolysis
phosphorylase ab


organism: Oryza sativa

substrate_name: amylopectin

substrate: amylopectin


organism: Papiliotrema laurentii


organism: Pyrococcus furiosus

substrate_name: maltotriose

proof_lines: 80°C, pH 6.5, direction of glucose 1-phosphate synthesis

substrate: maltotriose


organism: Rattus norvegicus

substrate_name: Unknown

substrate: Unknown


enzyme_display_name: Glycogen phosphorylase

organism: Aquifex aeolicus

substrate_name: maltotetraose

proof_lines: pH 6.5, 37°C, recombinant enzyme, synthesis reaction

proof_lines: pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction

enzyme: Glycogen phosphorylase

substrate: maltotetraose


enzyme_display_name: Alpha-1,4 glucan phosphorylase

organism: Chlamydomonas reinhardtii

proof_lines: presence of 1 mM orthophosphate, pH 7.0, 30°C

enzyme: Alpha-1,4 glucan phosphorylase


organism: Corynebacterium callunae

substrate_name: maltopentaose

assay_conditions_summary: pH 7

substrate: maltopentaose


enzyme_display_name: Glycogen phosphorylase, liver form

organism: Homo sapiens

proof_lines: pH 6.8, temperature not specified in the publication

enzyme: Glycogen phosphorylase, liver form


enzyme_display_name: Glycogen phosphorylase, muscle form

proof_lines: pH and temperature not specified in the publication

enzyme: Glycogen phosphorylase, muscle form


proof_lines: Pho1 with amylopectin as primer in the reaction, pH 6.5, 22°C

proof_lines: Pho1 with short alpha-glucan chain as primer in the reaction, pH 6.5, 22°C


enzyme_display_name: Alpha-glucan phosphorylase, H isozyme

organism: Triticum aestivum

proof_lines: pH 7.5, 30°C, degradation reaction, cytosolic isozyme

proof_lines: pH 7.5, 37°C, synthesis reaction, cytosolic isozyme

enzyme: Alpha-glucan phosphorylase, H isozyme


organism: Sulfurisphaera tokodaii str. 7

proof_lines: pH 6.2, 37°C, synthetic direction, mutant enzyme k308A


proof_lines: pH 6.2, 37°C, synthetic direction, mutant enzyme H185A


proof_lines: pH 6.2, 37°C, synthetic direction, mutant enzyme R303A


substrate_name: starch

proof_lines: pH 6.2, 37°C, phosphorolytic direction, wild-type enzyme

proof_lines: pH 6.2, 37°C, synthetic direction, wild-type enzyme

substrate: starch


organism: Sus scrofa

proof_lines: kidney dephosphophosphorylase, in the presence of 0.2 mg glycogen


organism: Thermus thermophilus

proof_lines:
phosphorolysis, at 30°C
phosphorolysis, at 70°C
synthesis


organism: Voandzeia subterranea

proof_lines: in the presence of 2 mM glucose 1-phosphate

proof_lines: in the presence of 10 mM glucose 1-phosphate


organism: Zea mays

proof_lines:
phosphorylase II
stromal phosphorylase, phosphorolysis
stromal phosphorylase, synthesis


organism: Saccharolobus solfataricus


organism: Saccharomyces cerevisiae

proof_lines:
synthesis
phosphorolysis
unphosphorylated glycogen phosphorylase


organism: Solanum tuberosum


organism: Spinacia oleracea

proof_lines:
cytoplasmic phosphorylase, pH 7.0
chloroplastphosphorylase, pH 8.0
cytoplasmic phosphorylase, pH 8.0


organism: Streptococcus salivarius

substrate_name: KF

proof_lines: glycogen synthesis, in the presence of NaF

substrate: KF


organism: Acetivibrio thermocellus

substrate_name: dextrin DP19

proof_lines:
pH 7.2, 50°C, degradation reaction
pH 6.8, 50°C, synthesis reaction

substrate: dextrin DP19


organism: Agrobacterium tumefaciens


organism: Badhamia polycephala


organism: Chlorella vulgaris

substrate_name: maltodecaheptaose

proof_lines:
phosphorolysis
synthesis
glucan synthesis

substrate: maltodecaheptaose


organism: Clostridium pasteurianum

proof_lines:
glucan synthesis
phosphorolysis of glucan


organism: Corynebacterium callunae

proof_lines: pH 7.0, 30°C, recombinant wild-type enzyme, phosphorolysis

proof_lines: pH 7.0, 30°C, recombinant wild-type enzyme, synthesis

proof_lines: pH 7.0, 30°C, recombinant wild-type enzyme and mutant H334A, phosphorolysis


proof_lines: pH 7.0, 30°C, recombinant mutant H334Q, phosphorolysis

proof_lines: pH 7.0, 30°C, recombinant mutant H334Q, synthesis


proof_lines: pH 7.0, 30°C, recombinant mutants H334A and H334Q, phosphorolysis


proof_lines: pH 7.0, 30°C, recombinant mutant H334N, phosphorolysis

proof_lines: pH 7.0, 30°C, recombinant mutant H334N, synthesis


proof_lines: pH 7.0, 30°C, recombinant mutant H334A, synthesis

proof_lines: pH 7.0, 30°C, recombinant mutant H334A, phosphorolysis


substrate_name: dextrin DP11

proof_lines:
phosphorolysis
synthesis
maltohexaose arsenolysis

substrate: dextrin DP11


organism: Cuscuta reflexa

proof_lines:
at 18.6 mM starch
at 1.0 mM glucose 1-phosphate
at 0.5 mM glucose 1-phosphate


enzyme_display_name: ornithine carbamoyltransferase

substrate_name: L-ornithine

enzyme: ornithine carbamoyltransferase

substrate: L-ornithine


organism: Squalus acanthias


organism: Sus scrofa


organism: Enterococcus faecalis

sequence_source: UniProtKB reviewed FASTA Q837U7 (PTC_ENTFA), Enterococcus faecalis strain V583; UniProt recommended EC 2.1.3.6.

proof_lines: recombinant wild-type enzyme, pH 8.5, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed FASTA Q837U7 (PTC_ENTFA), Enterococcus faecalis strain V583; UniProt recommended EC 2.1.3.6. and applied mutation signature Y230V/G231S/L232M/Y233G to reconstruct the assayed variant sequence.

proof_lines: recombinant enzyme mutant Y230V/G231S/L232M/Y233G, pH 8.5, 37°C


organism: Escherichia coli

substrate_name: L-ornithine, Carbamoyl phosphate

sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed FASTA P04391 (OTC1_ECOLI), Escherichia coli K-12 ornithine carbamoyltransferase subunit I; UniProt recommended EC 2.1.3.3. and applied mutation signature Y229S with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.

substrate: L-ornithine, Carbamoyl phosphate


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed FASTA P04391 (OTC1_ECOLI), Escherichia coli K-12 ornithine carbamoyltransferase subunit I; UniProt recommended EC 2.1.3.3. and applied mutation signature Y229F with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


substrate_name: Carbamoyl phosphate, L-ornithine

sequence_source: UniProtKB reviewed FASTA P04391 (OTC1_ECOLI), Escherichia coli K-12 ornithine carbamoyltransferase subunit I; UniProt recommended EC 2.1.3.3.

sequence_variant_note: Mutation signature E299D could not be applied safely because the resolved sequence does not match the expected reference residue(s).

substrate: Carbamoyl phosphate, L-ornithine


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed FASTA P04391 (OTC1_ECOLI), Escherichia coli K-12 ornithine carbamoyltransferase subunit I; UniProt recommended EC 2.1.3.3. and applied mutation signature Y160S with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Mutation signature D231A could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed FASTA P04391 (OTC1_ECOLI), Escherichia coli K-12 ornithine carbamoyltransferase subunit I; UniProt recommended EC 2.1.3.3. and applied mutation signature R57A with inferred residue-numbering offset +3 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed FASTA P04391 (OTC1_ECOLI), Escherichia coli K-12 ornithine carbamoyltransferase subunit I; UniProt recommended EC 2.1.3.3. and applied mutation signature S61A with inferred residue-numbering offset -5 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed FASTA P04391 (OTC1_ECOLI), Escherichia coli K-12 ornithine carbamoyltransferase subunit I; UniProt recommended EC 2.1.3.3. and applied mutation signature H272N with inferred residue-numbering offset +7 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed FASTA P04391 (OTC1_ECOLI), Escherichia coli K-12 ornithine carbamoyltransferase subunit I; UniProt recommended EC 2.1.3.3. and applied mutation signature Y160F with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed FASTA P04391 (OTC1_ECOLI), Escherichia coli K-12 ornithine carbamoyltransferase subunit I; UniProt recommended EC 2.1.3.3. and applied mutation signature D140N with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed FASTA P04391 (OTC1_ECOLI), Escherichia coli K-12 ornithine carbamoyltransferase subunit I; UniProt recommended EC 2.1.3.3. and applied mutation signature C273A with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed FASTA P04391 (OTC1_ECOLI), Escherichia coli K-12 ornithine carbamoyltransferase subunit I; UniProt recommended EC 2.1.3.3. and applied mutation signature E299Q with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed FASTA P04391 (OTC1_ECOLI), Escherichia coli K-12 ornithine carbamoyltransferase subunit I; UniProt recommended EC 2.1.3.3. and applied mutation signature Q104L with inferred residue-numbering offset +7 to reconstruct the assayed variant sequence.


organism: Lathyrus oleraceus

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


organism: Moritella abyssi

sequence_source: NCBI Protein/UniProt Q9K4Y9 linked from PMID:12644485

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


organism: Mycobacterium tuberculosis variant bovis

substrate_name: Carbamoyl phosphate

sequence_source: NCBI Protein CAA45530.1 linked from PMID:1406593

proof_lines: determined in the presence of 0.5 mM ornithine and 0.5 mM carbamoylphosphate

substrate: Carbamoyl phosphate


organism: Ovis aries

sequence_source: NCBI Protein/UniProt P84010 linked from PMID:14527149; PDB 1FB5 is also linked as the structural chain


organism: Piaractus mesopotamicus

proof_lines: liver enzyme, presence of putrescine, pH 7.0


organism: Pseudomonas aeruginosa

substrate_name: ornithine

sequence_source: NCBI Protein/UniProt P08308 linked from PMID:2118516

substrate: ornithine


proof_lines:
mutant arcB6254, pH 7.25
mutant arcB6240, pH 7.25


organism: Pseudomonas savastanoi

substrate_name: Diaminobutane

substrate: Diaminobutane


organism: Pyrococcus furiosus

sequence_source: Reconstructed from UniProt Q51742 linked from PMID:11208811

sequence_variant_note: Mutation signature A240D/E277G matches the mutant residue at offset +1 and the reference residue at offset -35; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines:
A240D/E277G double mutant, at 55°C
A240D/E277G double mutant, at 30°C


sequence_source: NCBI Protein/UniProt Q51742 linked from PMID:11208811

proof_lines:
at 55°C
at 30°C


sequence_variant_note: Mutation signature Y227C/E277G matches the mutant residue at offset +1 and the reference residue at offset -37; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines:
Y227C/E277G double mutant, at 55°C
Y227C/E277G double mutant, at 30°C


organism: Rattus norvegicus

proof_lines: at 30°C and pH 7.4, competitive vs. carbamoyl phosphate, noncompetitive vs. ornithine


enzyme_display_name: Ornithine carbamoyltransferase

enzyme_name_source: UniProtKB:P05150 targeted protein-name/EC/organism lookup

organism: Saccharomyces cerevisiae

sequence_variant_note: Resolved canonical sequence from UniProtKB:P05150 targeted OTCase EC 2.1.3.3 lookup; variant reconstructed by coordinate substitution and applied mutation signature K265A to reconstruct the assayed variant sequence.

enzyme: Ornithine carbamoyltransferase


sequence_variant_note: Resolved canonical sequence from UniProtKB:P05150 targeted OTCase EC 2.1.3.3 lookup; variant reconstructed by coordinate substitution and applied mutation signature D182N to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:P05150 targeted OTCase EC 2.1.3.3 lookup


sequence_variant_note: Resolved canonical sequence from UniProtKB:P05150 targeted OTCase EC 2.1.3.3 lookup; variant reconstructed by coordinate substitution and applied mutation signature K260R to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P05150 targeted OTCase EC 2.1.3.3 lookup; variant reconstructed by coordinate substitution and applied mutation signature E256Q to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P05150 targeted OTCase EC 2.1.3.3 lookup; variant reconstructed by coordinate substitution and applied mutation signature K263A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P05150 targeted OTCase EC 2.1.3.3 lookup; variant reconstructed by coordinate substitution and applied mutation signature E256A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P05150 targeted OTCase EC 2.1.3.3 lookup; variant reconstructed by coordinate substitution and applied mutation signature C191M/C194N/F197V to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P05150 targeted OTCase EC 2.1.3.3 lookup; variant reconstructed by coordinate substitution and applied mutation signature E123S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P05150 targeted OTCase EC 2.1.3.3 lookup; variant reconstructed by coordinate substitution and applied mutation signature K260A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P05150 targeted OTCase EC 2.1.3.3 lookup; variant reconstructed by coordinate substitution and applied mutation signature K268R to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P05150 targeted OTCase EC 2.1.3.3 lookup; variant reconstructed by coordinate substitution and applied mutation signature L290Q to reconstruct the assayed variant sequence.


sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.


organism: Homo sapiens

sequence_variant_note: Resolved canonical sequence from UniProtKB P00480 FASTA direct lookup; reconstructed by applying K307A to the canonical sequence and applied mutation signature K307A to reconstruct the assayed variant sequence.

proof_lines: mutant K307A, pH and temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB P00480 FASTA direct lookup; reconstructed by applying E98Q to the canonical sequence and applied mutation signature E98Q to reconstruct the assayed variant sequence.

proof_lines: mutant E98Q, pH and temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB P00480 FASTA direct lookup; reconstructed by applying A152V to the canonical sequence and applied mutation signature A152V to reconstruct the assayed variant sequence.

proof_lines: mutant A152V, pH and temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB P00480 FASTA direct lookup; reconstructed by applying H107N to the canonical sequence and applied mutation signature H107N to reconstruct the assayed variant sequence.

proof_lines: mutant H107N, pH and temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB P00480 FASTA direct lookup; reconstructed by applying K307E to the canonical sequence and applied mutation signature K307E to reconstruct the assayed variant sequence.

proof_lines: mutant K307E, pH and temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB P00480 FASTA direct lookup; reconstructed by applying E310Q to the canonical sequence and applied mutation signature E310Q to reconstruct the assayed variant sequence.

proof_lines: mutant E310Q, pH and temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB P00480 FASTA direct lookup; reconstructed by applying C303A to the canonical sequence and applied mutation signature C303A to reconstruct the assayed variant sequence.

proof_lines: mutant C303A, pH and temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB P00480 FASTA direct lookup; reconstructed by applying Q171A to the canonical sequence and applied mutation signature Q171A to reconstruct the assayed variant sequence.

proof_lines: mutant Q171A, pH and temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB P00480 FASTA direct lookup; reconstructed by applying D263N to the canonical sequence and applied mutation signature D263N to reconstruct the assayed variant sequence.

proof_lines: mutant D263N, pH and temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB P00480 FASTA direct lookup; reconstructed by applying H302L to the canonical sequence and applied mutation signature H302L to reconstruct the assayed variant sequence.

proof_lines: mutant H302L, pH and temperature not specified in the publication


sequence_source: UniProtKB P00480 FASTA direct lookup

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: wild-type enzyme, pH and temperature not specified in the publication


organism: Pyrococcus abyssi

sequence_source: UniProtKB O93656 FASTA direct lookup


organism: Thermus thermophilus

sequence_source: UniProtKB/Swiss-Prot:P96134; NCBI Protein:3183103; GenBank:Y10266


organism: Alnus glutinosa

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines:
at 2 mM carbamoyl phosphate
at 10 mM carbamoyl phosphate


organism: Bacillus subtilis


organism: Chlorella vulgaris var. autotrophica


organism: Escherichia coli

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


proof_lines: mixed or competitive vs. carbamoyl phosphate


organism: Giardia duodenalis

substrate_name: L-citrulline

sequence_source: UniProt O76458 via RCSB 3GRF entity 1 and PMID 19507245

substrate: L-citrulline


assay_conditions_summary: pH 8.98; buffer conditions

proof_lines: pH 8.98, TAPS buffer, carbonic anhydrase I mutant V62N and carbonic anhydrase I mutant H67N

proof_lines: pH 8.88, TAPS buffer, carbonic anhydrase I mutant V62N,H67N

proof_lines: pH 8.72, 1,2-dimethyimidazole buffer, carbonic anhydrase I mutant V62N,H67N


organism: Methanosarcina thermophila

proof_lines: pH 7.5, 25°C, variant E84A, 50 mM MOPS-Ac buffer

proof_lines: pH 7.5, 25°C, variant E84A, 50 mM MOPS buffer

proof_lines: pH 7.5, 25°C, variant E84A, 50 mM IMID buffer


sequence_source: UniProtKB:P40881 identity checked; row-specific variant sequence not accepted without exact kinetic evidence

smiles: O=C=O

assay_conditions_summary: pH 7.5; 298.15 K; buffer condition for CO2

proof_lines: pH 7.5, 25°C, variant E84Q, 50 mM MOPS buffer


assay_conditions_summary: pH 7.5; 298.15 K; buffer pH 7.5 MOPS

proof_lines: pH 7.5, 25°C, variant E84D, 50 mM MOPS buffer


enzyme_display_name: cholinesterase

substrate_name: Benzoylcholine

enzyme: cholinesterase

substrate: Benzoylcholine


substrate_name: butyrylthiocholine

substrate: butyrylthiocholine


substrate_name: succinylthiocholine

substrate: succinylthiocholine


proof_lines:
W82A mutant, pH 7, 25°C
pH 8, 25°C, W82A mutant


proof_lines:
A328Y mutant, pH 7, 25°C
pH 8, 25°C, A328Y mutant


enzyme_display_name: GDP-fucose protein O-fucosyltransferase 1

enzyme_name_source: UniProtKB P83337

organism: Cricetulus griseus

substrate_name: factor VII EGF

sequence_source: UniProtKB P83337 via NCBI pubmed_protein link from PMID 9525914

substrate: factor VII EGF


organism: Amblyomma americanum

substrate_name: butanoylthiocholine

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: butanoylthiocholine


organism: Berryteuthis magister

substrate_name: propionylthiocholine

substrate: propionylthiocholine


organism: Chelidonichthys lucerna

proof_lines: enzyme isolated from muscle, 25°C, pH not specified in the publication

proof_lines: enzyme isolated from brain, 25°C, pH not specified in the publication


organism: Diopatra neapolitana


organism: Eisenia andrei

substrate_name: propionylthiocholine iodide

substrate: propionylthiocholine iodide


organism: Equus caballus


organism: Gallotia galloti

substrate_name: butyrylthiocholine iodide

substrate: butyrylthiocholine iodide


organism: Gambusia yucatana

proof_lines: enzyme from muscle, pH 7.4, temperature not specified in the publication

proof_lines: enzyme from head, pH 7.4, temperature not specified in the publication


organism: Homo sapiens


proof_lines: A539T mutant, E497V/A539T mutant and E497V mutant, pH 7, 25°C


organism: Bos taurus

substrate_name: Unknown

sequence_source: UniProtKB accession P32749 direct lookup on 2026-06-26

substrate: Unknown


sequence_source: UniProtKB accession Q9N1N9 direct lookup on 2026-06-26


sequence_source: UniProtKB accession P81908 direct lookup on 2026-06-26


substrate_name: Butyrylthiocholine

sequence_source: UniProtKB accession P06276 direct lookup on 2026-06-26

smiles: CCCC(=O)SCC[N+](C)(C)C

substrate: Butyrylthiocholine


substrate_name: (-)-cocaine

sequence_variant_note: Mutation signature A328W could not be applied safely because the resolved sequence does not match the expected reference residue(s).

substrate: (-)-cocaine


proof_lines: wild-type, adapted method using probe IV, pH not specified in the publication, temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB accession P06276 direct lookup on 2026-06-26 and applied mutation signature A328W/Y332A with inferred residue-numbering offset +28 to reconstruct the assayed variant sequence.


sequence_variant_note: Mutation signature Y332A could not be applied safely because the resolved sequence does not match the expected reference residue(s).


substrate_name: benzoylthiocholine

proof_lines: reconmbinant enzyme produced in rice, pH not specified in the publication, temperature not specified in the publication

proof_lines: native enzyme, pH not specified in the publication, temperature not specified in the publication

substrate: benzoylthiocholine


proof_lines:
recombinant enzyme, pH 8.0, 22°C
recombinant enzyme, pH 7.4, 37°C
pH 7.4, 25°C


substrate_name: acetylthiocholine

smiles: CC(=O)SCC[N+](C)(C)C

substrate: acetylthiocholine


sequence_variant_note: Resolved canonical sequence from UniProt:P06276 reviewed CHLE_HUMAN canonical precursor; selected BChE mutation numbering matches mature chain with +28 signal-peptide offset and applied mutation signature A277W with inferred residue-numbering offset +28 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt:P06276 reviewed CHLE_HUMAN canonical precursor; selected BChE mutation numbering matches mature chain with +28 signal-peptide offset and applied mutation signature V288F with inferred residue-numbering offset +28 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt:P06276 reviewed CHLE_HUMAN canonical precursor; selected BChE mutation numbering matches mature chain with +28 signal-peptide offset and applied mutation signature Q119Y with inferred residue-numbering offset +28 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt:P06276 reviewed CHLE_HUMAN canonical precursor; selected BChE mutation numbering matches mature chain with +28 signal-peptide offset and applied mutation signature Q119Y/V288F/A328Y with inferred residue-numbering offset +28 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt:P06276 reviewed CHLE_HUMAN canonical precursor; selected BChE mutation numbering matches mature chain with +28 signal-peptide offset and applied mutation signature D70G with inferred residue-numbering offset +28 to reconstruct the assayed variant sequence.

proof_lines:
mutant D70G, pH 7.4, 25°C
pH 7.4, 25°C, D70G mutant
pH 7, 25°C, D70G mutant


sequence_variant_note: Resolved canonical sequence from UniProt:P06276 reviewed CHLE_HUMAN canonical precursor; selected BChE mutation numbering matches mature chain with +28 signal-peptide offset and applied mutation signature G333C with inferred residue-numbering offset +28 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt:P06276 reviewed CHLE_HUMAN canonical precursor; selected BChE mutation numbering matches mature chain with +28 signal-peptide offset and applied mutation signature R470W with inferred residue-numbering offset +28 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt:P06276 reviewed CHLE_HUMAN canonical precursor; selected BChE mutation numbering matches mature chain with +28 signal-peptide offset and applied mutation signature K12R with inferred residue-numbering offset +28 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt:P06276 reviewed CHLE_HUMAN canonical precursor; selected BChE mutation numbering matches mature chain with +28 signal-peptide offset and applied mutation signature V294M with inferred residue-numbering offset +28 to reconstruct the assayed variant sequence.


substrate_name: cocaine

sequence_variant_note: Resolved canonical sequence from UniProt:P06276 reviewed CHLE_HUMAN canonical precursor; selected BChE mutation numbering matches mature chain with +28 signal-peptide offset and applied mutation signature A328W/Y332A with inferred residue-numbering offset +28 to reconstruct the assayed variant sequence.

substrate: cocaine


substrate_name: Acetylcholine

sequence_variant_note: Resolved canonical sequence from UniProt:P06276 reviewed CHLE_HUMAN canonical precursor; selected BChE mutation numbering matches mature chain with +28 signal-peptide offset and applied mutation signature A199S/F227A/S287G/A328W/Y332G/E441D with inferred residue-numbering offset +28 to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme A199S/F227A/S287G/A328W/Y332G/E441D, pH not specified in the publication, at 25°C

substrate: Acetylcholine


sequence_variant_note: Resolved canonical sequence from UniProt:P06276 reviewed CHLE_HUMAN canonical precursor; selected BChE mutation numbering matches mature chain with +28 signal-peptide offset and applied mutation signature A199S/F227A/S287G/A328W/E441D with inferred residue-numbering offset +28 to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme A199S/F227A/S287G/A328W/E441D, pH not specified in the publication, at 25°C


substrate_name: butyryl thiocholine iodide

proof_lines: pH 7, mutant with only 5 N-glycosylation sites

substrate: butyryl thiocholine iodide


substrate_name: N-methylindoxyl acetate

substrate: N-methylindoxyl acetate


assay_conditions_summary: pH 5

proof_lines: pH 5, A328W and E197Q mutant, absence of excess substrate


sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: pH 5, wild-type enzyme, absence of excess substrate


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: pH 5, A328G mutant, absence of excess substrate


proof_lines: pH 5, L286W mutant, absence of excess substrate

proof_lines: pH 5, L286W mutant, presence of excess substrate


proof_lines: pH 5, A328F mutant, absence of excess substrate

proof_lines: pH 5, A328F mutant, presence of excess substrate


proof_lines: pH 5, Y332A mutant, presence of excess substrate

proof_lines: pH 5, Y332A mutant, absence of excess substrate