proof_lines: wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C


organism: Pseudomonas sp.

substrate_name: glutaryl-7-aminocephalosporanic acid

source_db: brenda

ki_display: Not reported

kcat_over_km_display: Not reported

source_license: CC BY 4.0

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution

substrate: glutaryl-7-aminocephalosporanic acid

source: brenda


enzyme_display_name: cathepsin L

organism: Homo sapiens

mutation_signature: Unknown

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

enzyme: cathepsin L


enzyme_display_name: glucose-1-phosphate adenylyltransferase

organism: Escherichia coli

substrate_name: ATP

protein_accession_database: UniProt

enzyme: glucose-1-phosphate adenylyltransferase

substrate: ATP


enzyme_display_name: alpha-glucosidase

organism: Ferroplasma acidiphilum

substrate_name: sucrose

kcat_display: Not reported

km_display: Not reported

enzyme: alpha-glucosidase

substrate: sucrose


substrate_name: maltose

proof_lines: 60°C, pH not specified in the publication

substrate: maltose


substrate_name: maltotriose

substrate: maltotriose


substrate_name: cephalosporin C

mutation_signature: deletion mutant D4(212-ADLA-215)

mutation_type: deletion

proof_lines: deletion mutant D4(212-ADLA-215), pH 8.5, 37°C

substrate: cephalosporin C


mutation_signature: deletion mutant D2(227-AM-228)

proof_lines: deletion mutant D2(227-AM-228), pH 8.5, 37°C


assay_conditions_summary: pH 8; 310.15 K; cofactor ATP


organism: Zea mays

substrate_name: alpha-D-glucose 1-phosphate

assay_conditions_summary: cofactor ATP

proof_lines: wild type BT2/SH2, 15 mM 3-phosphoglycerate

substrate: alpha-D-glucose 1-phosphate


proof_lines: mutant BT2/C114A, 15 mM 3-phosphoglycerate


enzyme_display_name: cellobiose dehydrogenase (acceptor)

organism: Phanerodontia chrysosporium

substrate_name: cellobiose

proof_lines: pH 4.5, 23°C, mutant enzyme H689N or mutant H689Q

enzyme: cellobiose dehydrogenase (acceptor)

substrate: cellobiose


substrate_name: lactose

substrate: lactose


assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

proof_lines: pH 7.4, 37°C, wild-type enzyme, in absence of 3-phosphoglycerate


proof_lines: mutant BT2/M172T, 15 mM 3-phosphoglycerate


proof_lines: wild-type, pH 7.4, 37°C, presence of phosphate


proof_lines: large subunit mutant D161G, 37°C, pH 7.4

proof_lines: large subunit mutant D161G, presence of 3-phosphoglycerate, 37°C, pH 7.4


proof_lines: large subunit mutant Q96G/D161G/A443R, 37°C, pH 7.4

proof_lines: large subunit mutant Q96G/D161G/A443R, presence of 3-phosphoglycerate, 37°C, pH 7.4


proof_lines: mutant BT2/S163F, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/T361C, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/C424V, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/H149S, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/D368S, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/P372A, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/V227R, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/Q213H, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/A508S, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/E438Q, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/V502T, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/C382F, 15 mM 3-phosphoglycerate


organism: Solanum tuberosum

assay_conditions_summary: 310.15 K; cofactor ATP


assay_conditions_summary: pH 7; 310.15 K; cofactor ATP

proof_lines: mutation P52L, large subunit, 37°C, pH 7.0


proof_lines: mutation K41R/T51K, large subunit, plus D143N, small subunit


proof_lines: mutation T51K, large subunit, plus D143N, small subunit


enzyme_display_name: feruloyl esterase

organism: Aspergillus niger

substrate_name: methyl sinapinate

enzyme: feruloyl esterase

substrate: methyl sinapinate


substrate_name: methyl ferulate

proof_lines:
pH 6.0, 37°C, native enzyme
pH 3.2
pH 6.1

substrate: methyl ferulate


substrate_name: methyl p-coumarate

substrate: methyl p-coumarate


proof_lines: mutation K41R, large subunit, plus D143N, small subunit


assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

proof_lines: large subunit mutant E38K/G101N, pH 7.5, 37°C


proof_lines: mutation D157L, large subunit, plus D143N, small subunit


organism: Unknown

substrate_name: ADP-glucose

assay_conditions_summary: cofactor ADP

substrate: ADP-glucose


proof_lines: pH 4.5, 23°C, mutant enzyme H689Q or mutant H689A

proof_lines: pH 4.5, 23°C, mutant enzyme H689Q or mutant enzyme H689A


assay_conditions_summary: pH 7; 310.15 K; cofactor ADP

proof_lines: mutation P52L, large subunit, plus mutation R350K, small subunit, 37°C, pH 7.0


proof_lines: mutation P52L, large subunit, plus mutation P112L, small subunit, 37°C, pH 7.0


proof_lines: mutation P52L, large subunit, plus mutation L46F, small subunit, 37°C, pH 7.0


organism: Arabidopsis thaliana

assay_conditions_summary: pH 7.9; cofactor ATP

proof_lines: pH 7.9, homotetramer APS1 in presence of 20 mM 3-phosphoglycerate

proof_lines: pH 7.9, APS1/APL4 in presence of 1 mM 3-phosphoglycerate

proof_lines: pH 7.9, APS1/APL2 in presence of 4 mM 3-phosphoglycerate


organism: Cereibacter sphaeroides

proof_lines: pH 8, 37°C, in absence of fructose 6-phosphate

proof_lines: pH 8, 37°C, in presence of fructose 6-phosphate

proof_lines: pH 8, 37°C, enzyme modified for 30 min with 5 mM 2,3-butanedione


organism: Nitrosomonas europaea

proof_lines:
pH 8.0, 37°C, presence of Mg2+
pH 8.0, 37°C, presence of Co2+
pH 8.0, 37°C, presence of Mn2+


organism: Rhodococcus jostii

proof_lines: hyperbolic behavior, Hill coefficient 1.3, presence of mannose 6-phosphate, pH 8.0, 37°C

proof_lines: hyperbolic behavior, Hill coefficient 0.9, pH 8.0, 37°C

proof_lines: hyperbolic behavior, Hill coefficient 1.1, presence of glucose 6-phosphate, pH 8.0, 37°C


organism: Solanum lycopersicum

substrate_name: diphosphate

assay_conditions_summary: pH 7.8; 310.15 K; cofactor ATP

proof_lines: pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L1 and small subunit S

proof_lines: pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L3 and small subunit S

substrate: diphosphate


proof_lines: large subunit mutant G101N, pH 7.5, 37°C


proof_lines: large subunit E370G/small subunit wild-type, pH 7.4, 37°C


assay_conditions_summary: pH 7.4; 298.15 K; cofactor ATP

proof_lines: heterodimer of small/large subunit, pH 7.4, 25°C

proof_lines: heterodimer of small/large subunit, presence of ATPgammaS, pH 7.4, 25°C


organism: Streptococcus mutans

proof_lines: S0.5 value, Hill coefficient 1.2, GlgC/GlgD complex, pH 8.0, 37°C

proof_lines: S0.5 value, Hill coefficient 1.5, subunit GlgC in presence of fructose 1,6-bisphosphate, pH 8.0, 37°C

proof_lines: S0.5 value, Hill coefficient 3.7, GlgC/GlgD complex, pH 8.0, 37°C


organism: Streptomyces coelicolor


proof_lines: chimeric enzyme, pH 7.4, 37°C, presence of 3-phosphoglycerate


proof_lines: mutant Q106A, S0.5 value, Hill coefficient 1.7, pH 8.0, 37°C

proof_lines: mutant Q106A, S0.5 value, Hill coefficient 1.2, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C

proof_lines: mutant Q106A, S0.5 value, Hill coefficient 1.3, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C


proof_lines: mutant Y114A, S0.5 value, Hill coefficient 0.3, pH 8.0, 37°C

proof_lines: mutant Y114A, S0.5 value, Hill coefficient 2.0, pH 8.0, 37°C

proof_lines: mutant Y114A, S0.5 value, Hill coefficient 0.2, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C


proof_lines: mutant W113A, S0.5 value, Hill coefficient 1.1, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C

proof_lines: mutant W113A, presence of 2 mM fructose 1,6-bisphosphate, pH 8.0, 37°C


proof_lines: mutant P103A, S0.5 value, Hill coefficient 1.1, pH 8.0, 37°C

proof_lines: mutant P103A, S0.5 value, Hill coefficient 1.5, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C

proof_lines: mutant P103A, S0.5 value, Hill coefficient 1.9, pH 8.0, 37°C


proof_lines: pH 7.9, APS1/APL4 in presence of 1 mM 3-phosphoglycerate


proof_lines: pH 7.5, 37°C, mutant TG-15, kinetic study


proof_lines: pH 7.6, 37°C, wild-type enzyme, comparison of Km of wild-type and mutant enzymes


proof_lines: mutant Q74A, presence of 2 mM fructose 1,6-bisphosphate, pH 8.0, 37°C


proof_lines: mutant R107A, S0.5 value, Hill coefficient 0.9, pH 8.0, 37°C

proof_lines: mutant R107A, S0.5 value, Hill coefficient 2.1, pH 8.0, 37°C

proof_lines: mutant R107A, S0.5 value, Hill coefficient 2.3, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C


proof_lines: mutant R115A, S0.5 value, Hill coefficient 1.7, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C

proof_lines: mutant R115A, S0.5 value, Hill coefficient 1.7, pH 8.0, 37°C

proof_lines: mutant R115A, S0.5 value, Hill coefficient 2.1, pH 8.0, 37°C


organism: Triticum aestivum

substrate_name: Unknown

ki_unit: mM

assay_conditions_summary: pH 8

proof_lines: endosperm enzyme in presence of 1 mM 3-phosphoglycerate

proof_lines: pH 8.0, in absence of 0.25 mM 3-phosphoglycerate

proof_lines: endosperm enzyme in presence of 0.25 mM 3-phosphoglycerate

substrate: Unknown


proof_lines: chimeric mutant, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C

proof_lines: chimeric mutant, substrate ATP, pH 7.4, 37°C

proof_lines: chimeric mutant, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C


assay_conditions_summary: pH 7.5; cofactor ATP


proof_lines: pH 7.0, 37°C, mutant enzyme P295G, in the presence of a saturating concentration of fructose 1,6-bisphosphate,


proof_lines: pH 7.0, 37°C, mutant enzyme G336D, in the presence of a saturating concentration of fructose 1,6-bisphosphate


proof_lines: pH 7.0, 37°C, mutant enzyme P295N, in the presence of a saturating concentration of fructose 1,6-bisphosphate


enzyme_display_name: laccase

organism: Rhizoctonia solani

enzyme: laccase


organism: Toxicodendron vernicifluum


organism: Escherichia coli

proof_lines: pH 7.0, 37°C, double mutant enzyme P295D/G336D


proof_lines: pH 7.0, 37°C, mutant enzyme P295Q, in the presence of a saturating concentration of fructose 1,6-bisphosphate


proof_lines: pH 7.0, 37°C, mutant enzyme P295D, in the presence of a saturating concentration of fructose 1,6-bisphosphate


proof_lines: pH 7.0, 37°C, mutant enzyme P295E, in the presence of a saturating concentration of fructose 1,6-bisphosphate


enzyme_display_name: peptidyl-dipeptidase A

organism: Homo sapiens

substrate_name: benzyloxycarbonyl-Phe-His-Leu

proof_lines: 37°C, pH 8.3, chimeric enzyme C417-579Ndom-ACE

enzyme: peptidyl-dipeptidase A

substrate: benzyloxycarbonyl-Phe-His-Leu


substrate_name: angiotensin I

substrate: angiotensin I


enzyme_display_name: procollagen-proline 4-dioxygenase

organism: Cavia porcellus

substrate_name: peptidyl L-proline

enzyme: procollagen-proline 4-dioxygenase

substrate: peptidyl L-proline


organism: Chlamydomonas reinhardtii

proof_lines: Mr 30000-40000 da, complex of recombinant subunits PHY-4.1 and PDI2

proof_lines: Mr 5000-10000 Da, complex of recombinant subunits PHY-4.1 and PDI2


organism: Gallus gallus

substrate_name: tert-butyloxycarbonyl-Pro-Pro-Gly-Pro

proof_lines: complete reaction with (Pro-Pro-Gly)5 as a peptide substrate

substrate: tert-butyloxycarbonyl-Pro-Pro-Gly-Pro


proof_lines: pH 7.8, 22°C, recombinant enzyme, HPLC-based assay

proof_lines: pH 7.8, 22°C, recombinant enzyme, fluoride-based assay


substrate_name: poly L-proline

proof_lines:
molecular weight 5000-10000 Da
molecular weight 30000-40000 Da
sequence present in extensin

substrate: poly L-proline


organism: Caenorhabditis elegans

substrate_name: 510-amino acid alpha1(I) collagen fragment


proof_lines: complex of recombinant subunits PHY-4.1 and PDI2


organism: Catharanthus roseus


organism: Struthio camelus australis


substrate_name: [Phe9,Arg10]angiotensin I

substrate: [Phe9,Arg10]angiotensin I


substrate_name: [Arg10]angiotensin I

substrate: [Arg10]angiotensin I


substrate_name: [Phe9]angiotensin I

substrate: [Phe9]angiotensin I


assay_conditions_summary: pH 8; 310.15 K; buffer Tris-NaCl buffer

proof_lines:
pH 8.0, 37°C, C-domain
pH 8.0, 37°C, N-domain
pH 7.4, 37°C


proof_lines: free acid, recombinant enzyme, and wild-type enzyme


organism: Drosophila melanogaster

substrate_name: hippuryl-His-Leu

proof_lines: C-catalytic domain of ACE, pH 7.5, temperature not specified in the publication

substrate: hippuryl-His-Leu


organism: Leishmania donovani


proof_lines:
pH 7.5, angiotensin-converting enzyme 2
pH, angiotensin-converting enzyme 2


organism: Paramecium bursaria Chlorella virus-1


organism: Phaseolus vulgaris

substrate_name: poly(L-Pro)

substrate: poly(L-Pro)


organism: Streptomyces griseoviridis


organism: Volvox carteri

substrate_name: poly(L-proline)

substrate: poly(L-proline)


enzyme_display_name: tyrosinase

organism: Aspergillus nidulans

substrate_name: Hydroquinone monomethylether

proof_lines: range of estimated values for crude or purified extracts

enzyme: tyrosinase

substrate: Hydroquinone monomethylether


organism: Mus musculus


organism: Mytilus edulis


organism: Panagrellus silusiae

substrate_name: Protocollagen

substrate: Protocollagen


organism: Paramecium bursaria Chlorella virus 1

proof_lines:
recombinant enzyme
MW: 13000, recombinant enzyme
MW: 40000, recombinant enzyme


organism: Homo sapiens

assay_conditions_summary: pH 6.8; 298.15 K; buffer 50 mM HEPES

proof_lines: mutant enzyme D543E, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: mutant enzyme V379S, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: mutant enzyme E304R, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: mutant enzyme V518T, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: mutant enzyme E376D, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


enzyme_display_name: formate dehydrogenase

organism: [Candida] boidinii

assay_conditions_summary: pH 5.5; 303.15 K; buffer 100 mM potassium phosphate; cofactor NAD+

proof_lines: pH 5.5, temperature not specified in the publication

proof_lines: with HCO3- as cosubstrate, pH 7.0, temperature not specified in the publication

proof_lines: in 100 mM potassium phosphate buffer at pH 7.5, at 30°C

enzyme: formate dehydrogenase


organism: Glycine max

substrate_name: NAD+

assay_conditions_summary: pH 7; buffer 0.1 M sodium phosphate; cofactor NAD+

proof_lines: mutant enzyme F290A, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication

substrate: NAD+


organism: Ogataea angusta

substrate_name: S-formylglutathione

assay_conditions_summary: cofactor NAD+

substrate: S-formylglutathione


organism: Thermothelomyces thermophilus

assay_conditions_summary: pH 6; 298.15 K; cofactor NAD+

proof_lines: pH 6.0, temperature not specified in the publication

proof_lines: in the absence of metal ion, at pH 6.5 and 25°C

proof_lines: in the presence of 0.01 mM Mo6+, at pH 6.5 and 25°C


substrate_name: Unknown

proof_lines: mutant enzyme V379S/V380T, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: mutant enzyme V380T, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: wild type enzyme, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: mutant enzyme T282S, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: mutant enzyme S516N, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: mutant enzyme F391Y, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


organism: Oryctolagus cuniculus

proof_lines: pH 7.5, temperature not specified in the publication

proof_lines: pH and temperature not specified in the publication


enzyme_display_name: alcohol dehydrogenase (NADP+)

organism: Moorella sp. HUC22-1

substrate_name: acetaldehyde

proof_lines:
isoform AdhA, pH 8.0, 60°C
isoform AdhB, pH 8.0, 60°C

enzyme: alcohol dehydrogenase (NADP+)

substrate: acetaldehyde


organism: Ovis aries

substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

proof_lines:
p-nitrobenzaldehyde as substrate
D-glucuronate as substrate
pyridine 3-aldehyde as substrate

substrate: NADPH


organism: Euglena gracilis

substrate_name: NADP+

assay_conditions_summary: pH 8.8; 303.15 K; cofactor NADP+

substrate: NADP+


organism: Gallus sp.

assay_conditions_summary: pH 6; 298.15 K; cofactor NADPH


substrate_name: phenylethanediol

proof_lines:
aldehyde reductase I
aldehyde reductase II
AR I

substrate: phenylethanediol


organism: Bos taurus

substrate_name: menadione

substrate: menadione


organism: Canis lupus familiaris

substrate_name: D-glucuronate

assay_conditions_summary: pH 6.2; 298.15 K; cofactor NADPH

proof_lines:
outer medulla enzyme
inner medulla enzyme
cortex enzyme

substrate: D-glucuronate


organism: Cricetinae

substrate_name: Succinic semialdehyde

substrate: Succinic semialdehyde


organism: Escherichia coli

substrate_name: butanaldehyde

assay_conditions_summary: pH 7; 310.15 K; cofactor NADPH

substrate: butanaldehyde


enzyme_display_name: catechol oxidase

organism: Streptomyces lavendulae

substrate_name: Catechol; O2

kcat_over_km_origin: calculated

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

km_unit: mM

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

data_origin: experimental

enzyme: catechol oxidase

substrate: Catechol; O2


organism: Pelophylax perezi

substrate_name: Butanal

assay_conditions_summary: pH 10

proof_lines:
ethanol as substrate
m-nitrobenzaldehyde as substrate
at pH 10

substrate: Butanal


organism: Phycomyces blakesleeanus

substrate_name: n-butanol

assay_conditions_summary: cofactor NADP+

substrate: n-butanol


organism: Platyrrhini


organism: Pseudothermotoga hypogea


organism: Agroathelia rolfsii

substrate_name: cellobiose

proof_lines: electron acceptors methylene blue or methylene green

proof_lines: electron acceptor cytochrome c from horseheart

substrate: cellobiose


organism: Arabidopsis thaliana

substrate_name: cellotetraose

substrate: cellotetraose


organism: Thermococcus paralvinellae

assay_conditions_summary: pH 8; 353.15 K; cofactor NADPH


organism: Thermococcus sp.

substrate_name: butanol

substrate: butanol


organism: Trypanosoma cruzi

substrate_name: n-Propanol

proof_lines:
benzaldehyde as substrate
benzyl alcohol as substrate
n-propanol as substrate

substrate: n-Propanol


organism: Vigna radiata

substrate_name: coniferylaldehyde

substrate: coniferylaldehyde


organism: Pyrococcus furiosus

substrate_name: methylglyoxal

proof_lines: 80°C, pH not specified in the publication

substrate: methylglyoxal


organism: Rattus norvegicus

substrate_name: hexadecanal

proof_lines:
enzyme from liver and kidney
enzyme from kidney, brain or liver
enzyme from brain or liver

substrate: hexadecanal


organism: Saccharomyces cerevisiae

substrate_name: cinnamyl alcohol

assay_conditions_summary: pH 10; 298.15 K; cofactor NADPH

proof_lines:
pH 10.0, 25°C
pH 7.0, 25°C
coenzyme NADPH

substrate: cinnamyl alcohol


organism: Solanum tuberosum


organism: Coniophora puteana (Schum ex Fr) Karsten

substrate_name: Unknown

substrate: Unknown


organism: Coprinopsis cinerea


organism: Fungi

substrate_name: cytochrome c

substrate: cytochrome c


organism: Hypoxylon haematostroma

substrate_name: D-glucose

substrate: D-glucose


organism: Monilia sitophila


organism: Mycothermus thermophilus

substrate_name: xylobiose

substrate: xylobiose


organism: Neurospora crassa

proof_lines: recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C


organism: Sporobolomyces salmonicolor

substrate_name: octyl 4-chloro-3-oxobutanoate

substrate: octyl 4-chloro-3-oxobutanoate


organism: Sus scrofa

proof_lines:
reductase I
low-Km aldehyde reductase
high-Km aldehyde reductase


organism: Synechocystis sp.

assay_conditions_summary: pH 8


organism: Thermoanaerobacter brockii


organism: Thermoanaerobacter ethanolicus

substrate_name: ethanol

proof_lines:
2° Adh
1°Adh, acetaldehyde as substrate
2° Adh, butan-2-ol as substrate

substrate: ethanol


organism: Thermococcus hydrothermalis

substrate_name: benzyl alcohol

proof_lines:
co-substrate NADP
benzyl aclohol as substrate
benzaldehyde as substrate

substrate: benzyl alcohol


organism: Thermococcus litoralis

substrate_name: NADPH

substrate: NADPH


organism: Phanerochaete sordida

substrate_name: lactose

substrate: lactose


organism: Phanerodontia chrysosporium

substrate_name: cellobiose

proof_lines: pH 4.5, 23°C, recombinant wild-type enzyme

substrate: cellobiose


substrate_name: cellulose

proof_lines:
ferricyanide as electron acceptor
pH 4.5, 22°C
pH 4.0, 30°C, recombinant enzyme

substrate: cellulose


organism: Podospora anserina

substrate_name: cellotriose

substrate: cellotriose


enzyme_display_name: monoamine oxidase

organism: Avena sativa

enzyme: monoamine oxidase


organism: Bos taurus

proof_lines:
pH 7.2, 30°C
MAO-B


organism: Coregonus lavaretus ludoga

substrate_name: serotonin

assay_conditions_summary: pH 7.4; 296.15 K; buffer 0.01 M phosphate

proof_lines: in 0.01 M phosphate buffer, pH 7.4, at 23°C

substrate: serotonin


organism: Danio rerio

proof_lines: at 25°C in 50 mM potassium phosphate, pH 7.4


organism: Thermothelomyces fergusii

proof_lines: recombinant enzyme from Trichoderma reesei, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C

proof_lines: recombinant enzyme from Escherichia coli, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C


organism: Thermothelomyces heterothallicus

proof_lines:
at 45°C
at 25°C
at pH 4.5 and 45°C


organism: Thermothelomyces myriococcoides

substrate_name: ferricytochrome c

assay_conditions_summary: pH 5.5; 303.15 K; buffer 100 mM sodium acetate

proof_lines: in the presence of 30 mM NaCl, at pH 5.5 and 30°C

proof_lines: in the presence of 30 mM BaCl2, at pH 5.5 and 30°C

proof_lines: in the presence of 30 mM CaCl2, at pH 7.5 and 30°C

substrate: ferricytochrome c


organism: Trametes cinnabarina


organism: Equus caballus


organism: Homo sapiens

substrate_name: benzylamine

proof_lines:
recombinant MAO-B
MAO-A
11°C

substrate: benzylamine


organism: Huso huso


organism: Katsuwonus pelamis


organism: Acipenser gueldenstaedtii

substrate_name: N-methylhistamine

substrate: N-methylhistamine


organism: Acipenser persicus


organism: Acipenser stellatus


organism: Trametes hirsuta

proof_lines:
at pH 4.5
electron donor
electron acceptor


organism: Trametes pubescens

substrate_name: cellotetraose

proof_lines: pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol

substrate: cellotetraose


organism: Trametes versicolor


organism: Trametes villosa


enzyme_display_name: sucrose synthase

organism: Acidithiobacillus caldus

substrate_name: UDP

assay_conditions_summary: pH 7; 333.15 K; cofactor ADP

enzyme: sucrose synthase

substrate: UDP


organism: Anabaena sp.

substrate_name: UDP-alpha-D-glucose

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ADP

proof_lines: with ADP-alpha-D-glucose as cosubstrate, at pH 7.5 and 30°C

substrate: UDP-alpha-D-glucose


organism: Arabidopsis thaliana

assay_conditions_summary: pH 6; 298.15 K; cofactor ADP

proof_lines: isoform SUS1, with UDP as cosubstrate, at pH 6.0 and 25°C


organism: Beta vulgaris

proof_lines: isoform SUS1, with UDP as cosubstrate, at pH 6.5 and 35°C


organism: Ipomoea batatas

substrate_name: ADP

assay_conditions_summary: pH 6; 310.15 K; cofactor ADP

proof_lines: with UDP-alpha-D-glucose as cosubstrate, at pH 7.5 and 37°C

substrate: ADP


organism: Lathyrus oleraceus

proof_lines: isoform SUS3, with UDP-alpha-D-glucose as cosubstrate, at pH 9.0 and 30°C


organism: Manihot esculenta

substrate_name: D-fructose

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ADP

proof_lines: with ADP as cosubstrate, at pH 7.5 and 37°C

substrate: D-fructose


organism: Nicotiana tabacum

substrate_name: sucrose

proof_lines:
SuSy2, 25°C
SuSy1, 25°C
25°C, isoform SuSy2

substrate: sucrose


organism: Schizophyllum commune


organism: Stachybotrys bisbyi

substrate_name: D-glucose

substrate: D-glucose


organism: Daucus carota


organism: Glycine max

substrate_name: UDPglucose

assay_conditions_summary: cofactor ADP

proof_lines:
cosubstrate CDP
cosubstrate ADPglucose

substrate: UDPglucose


organism: Helianthus tuberosus

proof_lines: with UDP-alpha-D-glucose as cosubstrate, at pH 8.0 and 30°C

proof_lines: with UDP as cosubstrate, at pH 8.3 and 30°C


organism: Hordeum vulgare

assay_conditions_summary: pH 7; 310.15 K; cofactor ADP

proof_lines: with ADP as cosubstrate, at pH 7.0 and 37°C

proof_lines: with UDP as cosubstrate, at pH 7.0 and 37°C


organism: Macaca fuscata

substrate_name: Unknown

substrate: Unknown


organism: Mesocricetus auratus

substrate_name: Phenylethylamine

substrate: Phenylethylamine


organism: Mus musculus


organism: Narcissus tazetta


organism: Oncorhynchus keta


organism: Papio anubis

proof_lines: pH not specified in the publication, temperature not specified in the publication, MAO-B


organism: Papio hamadryas

proof_lines: with 1-methyl-4-(1-methylpyrrol-2-yl)1,2,3,6-tetrahydropyridine as substrate


organism: Papio sp.


organism: Rattus norvegicus

assay_conditions_summary: pH 9; 298.15 K; buffer 50 mM potassium phosphate assay

proof_lines: isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C


organism: Sus scrofa


organism: Todarodes pacificus

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Zea mays

assay_conditions_summary: pH 6.5; 303.15 K; cofactor ADP

proof_lines:
cosubstrate UDPglucose, ADP
at pH 6.5 and 30°C
at pH 8.0 and 30°C


organism: Nitrosomonas europaea

substrate_name: ADP-alpha-D-glucose

assay_conditions_summary: pH 8; 310.15 K; cofactor ADP

proof_lines: with ADP as cosubstrate, at pH 7.0 and 60°C

proof_lines: with ADP-alpha-D-glucose as cosubstrate, at pH 8.0 and 37°C

substrate: ADP-alpha-D-glucose


organism: Oryza sativa

proof_lines: with UDP-alpha-D-glucose as cosubstrate, at pH 8.0 and 37°C


organism: Populus tremuloides


organism: Prunus persica

proof_lines: with UDP-alpha-D-glucose as cosubstrate, at pH 8.5 and 25°C


organism: Pyrus pyrifolia

assay_conditions_summary: pH 7; 303.15 K; cofactor ADP

proof_lines: isoform SUS2, with UDP as cosubstrate, at pH 7.0 and 30°C

proof_lines: isoform SUS2, with UDP-alpha-D-glucose as cosubstrate, at pH 8.0 and 30°C


organism: Ricinus communis

proof_lines: in the presence of 0.2 mM trehalose 6-phosphate, at pH 7.0 and 25°C

proof_lines: in the absence of trehalose 6-phosphate, at pH 7.0 and 25°C


organism: Saccharum officinarum

assay_conditions_summary: pH 7

proof_lines:
pH 7.0, isoform SuSyC
pH 7.5, isoform SuSyC
pH 7.5, isoform SuSyB


organism: Saccharum sp.

proof_lines: at pH 7.0, temperature not specified in the publication

proof_lines: at pH 7.3, temperature not specified in the publication


enzyme_display_name: anthranilate phosphoribosyltransferase

organism: Fructobacillus fructosus

substrate_name: 5-phospho-alpha-D-ribose 1-diphosphate

enzyme: anthranilate phosphoribosyltransferase

substrate: 5-phospho-alpha-D-ribose 1-diphosphate


organism: Methanocella conradii


organism: Methanococcus voltae


organism: Ogataea henricii

substrate_name: anthranilate

substrate: anthranilate


organism: Solanum chmielewskii

proof_lines: with UDP as cosubstrate, at pH 6.5 and 25°C


organism: Solanum tuberosum

proof_lines: isoform SUS1, with UDP-alpha-D-glucose as cosubstrate, at pH 9.0 and 30°C


organism: Tetradesmus obliquus


organism: Thermosynechococcus vestitus

proof_lines: with ADP-alpha-D-glucose as cosubstrate, at pH 7.0 and 37°C

proof_lines: pH 7.0, 37°C, purified recombinant enzyme, with ADP in sucrose cleavage


organism: Acetomicrobium mobile


organism: Citrus sinensis


organism: Coprococcus eutactus


organism: Escherichia coli

proof_lines:
complex
component II


organism: Triticum aestivum

proof_lines:
at pH 6.5 and 37 °C
cosubstrate UDP


organism: Vicia faba

assay_conditions_summary: pH 7; 298.15 K; buffer cosubstrate UDP; cofactor ADP

proof_lines: with UDP as cosubstrate, at pH 7.0 and 25°C


organism: Vigna radiata

assay_conditions_summary: pH 7.5; 299.15 K; cofactor ADP

proof_lines: with UDP-alpha-D-glucose as cosubstrate, at pH 7.5, temperature not specified in the publication


organism: Vigna radiata var. radiata

substrate_name: GDP

proof_lines:
cosubstrate ADP
cosubstrate TDP

substrate: GDP


organism: Staphylococcus aureus


organism: Staphylococcus haemolyticus


enzyme_display_name: hypoxanthine phosphoribosyltransferase

organism: Artemia sp.

substrate_name: guanine

enzyme: hypoxanthine phosphoribosyltransferase

substrate: guanine


organism: Bos taurus


organism: Caldanaerobacter subterraneus subsp. tengcongensis

proof_lines: pH 7.4, 12 mM MgCl2, in presence of 0.05 mM IMP

proof_lines: pH 7.4, 12 mM MgCl2, versus 5-phospho-alpha-D-ribose 1-diphosphate


organism: Cricetulus griseus

substrate_name: hypoxanthine

substrate: hypoxanthine


organism: Pelodictyon luteolum


organism: Petrotoga mobilis


organism: Physcomitrium patens

proof_lines: Ki above 5 mM, wild type enzyme, at pH 7.4 and 30°C


organism: Pistacia vera


proof_lines:
at pH 7.4 and 25°C
Ki above 0.01 mM, at pH 7.4 and 25°C


organism: Gallus gallus


organism: Giardia duodenalis

proof_lines:
wild-type enzyme
wild-type enzyme, reverse reaction
wild-type, forward reaction


organism: Helicobacter pylori

proof_lines: pH and temperature not specified in the publication


organism: Homo sapiens


substrate_name: inosine monophosphate

proof_lines: purified recombinant chimeric enzyme DS1

substrate: inosine monophosphate


organism: Methanococcus voltae


organism: Saccharolobus solfataricus

proof_lines:
at 87°C
at 60°C
at 25°C


organism: Saccharomyces cerevisiae


organism: Salmonella enterica subsp. enterica serovar Typhimurium


organism: Selaginella moellendorffii


organism: Plasmodium vivax


organism: Pyrococcus horikoshii

substrate_name: xanthine

substrate: xanthine


organism: Schistosoma mansoni

proof_lines:
recombinant enzyme
native enzyme


organism: Mycobacterium tuberculosis

proof_lines: pH 7.4, 25°C, recombinant enzyme, with hypoxanthine

proof_lines: pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate

proof_lines: pH 7.4, 25°C, recombinant enzyme, with guanine


organism: Plasmodium chabaudi


organism: Plasmodium falciparum

proof_lines: at pH 7.4, temperature not specified in the publication

proof_lines: recombinant enzyme, with 5-phosphoribosyl 1-diphosphate, pH 8.5


organism: Plasmodium lophurae


organism: Schizosaccharomyces pombe


organism: Streptomyces cyanogenus


organism: Tritrichomonas suis


proof_lines: mutant K134S, competitive versus 5-phospho-alpha-D-ribose 1-diphosphate


organism: Trypanosoma cruzi

mutation_signature: loop II-deletion mutant


substrate_name: diphosphate

proof_lines: pH 7.5, 37°C, recombinant enzyme, with GMP

proof_lines: pH 7.5, 37°C, recombinant enzyme, with IMP

substrate: diphosphate


enzyme_display_name: glutathione transferase

organism: Hevea brasiliensis

enzyme: glutathione transferase


organism: Schistosoma japonicum

proof_lines: 25°C, 20 mM sodium phosphate buffer, pH 7.0, 0.5 mM glutathione

proof_lines: 4°C, 20 mM sodium phosphate buffer, pH 6.5, 0.5 mM glutathione


enzyme_display_name: creatine kinase

organism: Homo sapiens

substrate_name: ATP

assay_conditions_summary: cofactor ATP

proof_lines:
mutant D54G
mutant enzyme D54G

enzyme: creatine kinase

substrate: ATP


substrate_name: Creatine

assay_conditions_summary: pH 8; 298.15 K; cofactor ATP

proof_lines: co-substrate: ATP, wild-type hMMCK, pH and temperature not specified in the publication

proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, wild-type

substrate: Creatine


assay_conditions_summary: 298.15 K; cofactor ATP

proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant K304T


organism: Lethenteron camtschaticum

proof_lines:
cytoplasmic isoform M2, 25°C
cytoplasmic isoform M1, 25°C


organism: Mus musculus

assay_conditions_summary: pH 6.5; 298.15 K; cofactor ATP

proof_lines:
cytoplasmic isoform M, 25°C
cytoplasmic isoform B, 25°C
mitochondrial isoform, 25°C


organism: Pagrus major

substrate_name: ADP

substrate: ADP


organism: Pelodiscus sinensis

assay_conditions_summary: pH 9; 293.15 K; cofactor ATP

proof_lines:
pH 9.0, 20°C
versus ATP, pH 9.0, 20°C
versus creatine, pH 9.0, 20°C


organism: Physeter macrocephalus

proof_lines:
muscle-type isoform MCK
mitochondrial isoform sMiCK


enzyme_display_name: glucose-1-phosphate adenylyltransferase

organism: Aeromonas caviae

proof_lines: in presence of 0.5 mM fructose 6-phosphate

enzyme: glucose-1-phosphate adenylyltransferase


organism: Aeromonas hydrophila

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP

proof_lines: pH 8.0, 37°C, in presence of fructose 6-phosphate

proof_lines: pH 8.0, 37°C, in presence of fructose 1,6-bisphosphate


organism: Agrobacterium tumefaciens

substrate_name: alpha-D-glucose 1-phosphate

proof_lines: pH 8.0, 37°C, wild-type enzyme in presence of 0.3 mM fructose-1,6-bisphosphate

substrate: alpha-D-glucose 1-phosphate


organism: Anabaena sp.

assay_conditions_summary: pH 7.5; 278.15 K; cofactor ATP

proof_lines:
pH 7.5, 5°C, kinetic study
pH 7.0, 37°C, kinetic study
pH 7.5, 30°C, kinetic study


organism: Rattus norvegicus

substrate_name: creatine phosphate

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

proof_lines: 0.005 mM organotellurium, preincubation time 5 min, mitochondrial fraction, pH 7.5, 37°C

substrate: creatine phosphate


organism: Tetronarce californica

assay_conditions_summary: 303.15 K; cofactor ATP


organism: Torpedo marmorata

assay_conditions_summary: cofactor ADP

proof_lines: acetylcholine receptor membrane-asscociated enzyme


organism: Urocitellus richardsonii

proof_lines:
25°C, myosin bound enzyme
25°C, hibernating squirrel
25°C, euthermic squirrel


organism: Dendronephthya gigantea

proof_lines:
mitochondrial isoform, 25°C
mitochondrial isoform M2, 25°C


organism: Gallus gallus


organism: Ginglymostoma cirratum


organism: Hediste diversicolor

proof_lines:
mitochondrial isoform, 25°C
cytoplasmic isoform, 25°C
cytoplasmic isoform M1, 25°C


organism: Bos taurus

assay_conditions_summary: pH 7.4; 303.15 K; cofactor ATP

proof_lines:
30°C
ADP in form of MgADP-
pH 7.4, dimeric form


organism: Chaenocephalus aceratus

assay_conditions_summary: pH 7.6; 273.65 K; cofactor ADP


organism: Cryptosporidium muris

proof_lines: pH and temperature not specified in the publication


organism: Danio rerio

proof_lines:
cytoplasmic isoform M2, 25°C
cytoplasmic isoform M1, 25°C
cytoplasmic isoform B, 25°C


organism: Auxenochlorella pyrenoidosa

assay_conditions_summary: pH 8.5; cofactor ADP

proof_lines: pH 8.5, in presence of 2 mM 3-phosphoglycerate

proof_lines: pH 8.5, in absence of 3-phosphoglycerate


organism: Cereibacter sphaeroides

proof_lines: in absence of 0.1 mM fructose 6-phosphate

proof_lines: in absence of 0.01 mM fructose 6-phosphate


organism: Chlamydomonas reinhardtii

proof_lines: pH 8.0, 37°C, in presence of 2.5 mM 3-phosphoglycerate

proof_lines: pH 8.0, 37°C, in absence of 3-phosphoglycerate


organism: Citrobacter freundii

assay_conditions_summary: pH 8.4; 310.15 K; cofactor ATP

proof_lines: pH 8.4, 37°C, influence of activators on Km


organism: Escherichia coli

assay_conditions_summary: pH 7.6; 310.15 K; cofactor ADP


assay_conditions_summary: pH 7; 310.15 K; cofactor ATP

proof_lines: pH 7.0, 37°C, strain AC70R1, kinetic study

proof_lines: S0.5 value, Hill coefficient 1.0, presence of 25 mM pyruvate and 0.01 mM fructose 1,6-bisphosphate, pH 8.0, 37°C


organism: Hafnia alvei

proof_lines: pH 8.0, 37°C, in absence of phosphoenolpyruvate

proof_lines: pH 8.0, 37°C, in presence of phosphoenolpyruvate


organism: Hordeum vulgare

assay_conditions_summary: pH 8; cofactor ATP

proof_lines: pH 8.0, in presence of 2.5 mM 3-phosphoglycerate

proof_lines: pH 8.0, in absence of 3-phosphoglycerate


enzyme_display_name: flavivirin

organism: West Nile virus

assay_conditions_summary: pH 8; 295.15 K; buffer 20 mM Tris/HCl

proof_lines: mutant enzyme R76L, in 20 mM Tris/HCl buffer, pH 8.0, at 22°C

enzyme: flavivirin


proof_lines: mutant enzyme T52V, in 20 mM Tris/HCl buffer, pH 8.0, at 22°C


organism: Solanum tuberosum

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

proof_lines: the phosphate inhibition pattern of Mos(1-198) is complex and biphasic. Inhibition at relatively high phopshate concentrations is less than predicted at low phosphate concentrations, the calculated Ki for phosphate is about 1 mM before 50%

proof_lines: chimeric mutant, substrate ATP, pH 7.4, 37°C

proof_lines: chimeric mutant, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C


proof_lines: chimeric enzyme, pH 7.4, 37°C, presence of 3-phosphoglycerate

proof_lines: chimeric enzyme, pH 7.4, 37°C, presence of phosphate


organism: Spinacia oleracea

assay_conditions_summary: pH 7.3; 310.15 K; cofactor ATP

proof_lines: pH 7.3, 37°C, in presence of 1 mM 3-phosphoglycerate

proof_lines: pH 7.3, 37°C, in absence of 3-phosphoglycerate

proof_lines: pH 7.5, in presence of 1 mM 3-phosphoglycerate


organism: Synechococcus sp.


enzyme_display_name: carboxylesterase

organism: uncultured bacterium

substrate_name: 4-nitrophenyl butyrate

proof_lines: recombinant wild-type enzyme, pH 8.5, 30°C

enzyme: carboxylesterase

substrate: 4-nitrophenyl butyrate


enzyme_display_name: acetylcholinesterase

organism: Anopheles gambiae

substrate_name: Acetylcholine

enzyme: acetylcholinesterase

substrate: Acetylcholine


organism: Apis mellifera

substrate_name: acetylthiocholine

proof_lines: isoform AChE2, at 30°C, pH not specified in the publication

proof_lines: isoform AChE1, at 30°C, pH not specified in the publication

substrate: acetylthiocholine


organism: Arapaima gigas

assay_conditions_summary: pH 8; buffer 0.5 M Tris-HCl

proof_lines: in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication


organism: Bactrocera dorsalis

substrate_name: propionylthiocholine

proof_lines:
25°C
enzyme from larva, at pH 7.4 and 37°C
enzyme from pupa, at pH 7.4 and 37°C

substrate: propionylthiocholine


organism: Lens culinaris

proof_lines:
pH 7.4, 37°C, long day photoperiod
pH 7.4, 37°C, short day photoperiod


organism: Mycolicibacterium smegmatis


organism: Oryza sativa

proof_lines: S0.5 value, large subunit mutant T139I with wild-type small subunit, 37°C, pH 7.0

proof_lines: S0.5 value, large subunit mutant A171V with wild-type small subunit, 37°C, pH 7.0

proof_lines: S0.5 value, wild-type small subunit alone, 37°C, pH 7.0


proof_lines: pH 8.0, in presence of 5 mM 3-phosphoglycerate

proof_lines: pH 8.0, in presence of 1 mM 3-phosphoglycerate

proof_lines: pH 8.0, in presence of 0.5 mM 3-phosphoglycerate


organism: Rhodobacter capsulatus

substrate_name: Unknown

substrate: Unknown


organism: Rhodocyclus tenuis

proof_lines:
in presence of 0.05 mM pyruvate
in absence of pyruvate
in presence of 1 mM pyruvate


organism: Salmonella enterica subsp. enterica serovar Typhimurium

proof_lines: pH 8.0, 37°C, strain JP102, kinetic study

proof_lines: pH 8.4, 37°C, influence of activators on Km

proof_lines: pH 8.0, 37°C, strain LT-2, kinetic study


organism: Serratia liquefaciens


organism: Eledone moschata

proof_lines:
form II
form I


organism: Gallotia galloti

substrate_name: acetylcholine iodide

substrate: acetylcholine iodide


organism: Gambusia yucatana

proof_lines: enzyme from head, pH 7.4, temperature not specified in the publication

proof_lines: enzyme from muscle, pH 7.4, temperature not specified in the publication


organism: Gammarus pulex


organism: Synechocystis sp.

proof_lines: pH 7.0, 37°C, in presence of 2.5 mM 3-phosphoglycerate

proof_lines: pH 7.0, 37°C, in absence of 3-phosphoglycerate


organism: Thermus caldophilus

assay_conditions_summary: pH 7.6; 348.15 K; cofactor ATP


organism: Zea mays

proof_lines: pH 7.4, 37°C, enzyme from Zea mays endosperm


proof_lines:
pH 7.4, 37°C, mutant BT2-TI
pH 7.4, 37°C, mutant MP
pH 7.4, 37°C, mutant MP-TI


organism: Blattella germanica

substrate_name: acetylthiocholine iodide

proof_lines: recombinant enzyme AChE1, pH not specified in the publication, 30°C

proof_lines: recombinant enzyme AChE2, pH not specified in the publication, 30°C

substrate: acetylthiocholine iodide


organism: Bos taurus


organism: Carassius auratus auratus


organism: Cavia porcellus


organism: Colossoma macropomum

assay_conditions_summary: pH 8; 295.15 K; buffer 0.5 M Tris-HCl

proof_lines: in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication

proof_lines: in 0.5 mM Tris-HCl buffer, pH 7.4, at 22°C


organism: Dendrobaena veneta


organism: Diapterus auratus

proof_lines: pH 7.4, temperature not specified in the publication


organism: Diapterus rhombeus


organism: Diopatra neapolitana


organism: Drosophila melanogaster

proof_lines:
from eel
from human
from Drosophila


organism: Eisenia andrei

substrate_name: propionylthiocholine iodide

substrate: propionylthiocholine iodide


organism: Electrophorus electricus

proof_lines:
pH 7.4, 30°C, soluble free enzyme
pH 7.2
25°C, pH 8.0, untreated preparation


organism: Serratia marcescens

proof_lines:
pH 8.0, 37°C, strain ATCC 15365
pH 8.0, 37°C, strain ATCC 274


organism: Solanum lycopersicum

proof_lines: pH 7.4, 37°C, fruit enzyme, in presence of 3-phosphoglycerate

proof_lines: pH 7.4, 37°C, leaf enzyme, in presence of 3-phosphoglycerate


organism: Solanum tuberosum

proof_lines: mutation P52L, large subunit, plus mutation P308L, small subunit, 37°C, pH 7.0


proof_lines: reduced wild-type, pH 7.4, 37°C, presence of phosphate

proof_lines: oxidized wild-type, pH 7.4, 37°C, presence of phosphate


organism: Centropomus undecimalis


organism: Chelidonichthys lucerna

proof_lines: enzyme isolated from muscle, 25°C, pH not specified in the publication


organism: Cichla ocellaris


organism: Cimex lectularius


organism: Mytilus galloprovincialis

substrate_name: butyrylthiocholine

substrate: butyrylthiocholine


organism: Nephotettix cincticeps Uhler

proof_lines: pH 7.5, 25°C, N-methyl carbamate-susceptible strain

proof_lines: pH 7.5, 25°C, N-methyl carbamate-resistant strain

proof_lines: pH 7.5, 25°C, N-methyl carbamate-resisitant strain


organism: Nippostrongylus brasiliensis


organism: Oncorhynchus mykiss


organism: Magallana hongkongensis

proof_lines: in 80 mM sodium phosphate (pH 7.4), at 37°C


organism: Mus musculus


proof_lines: pH 7.2, 25°C, presence of aflatoxin B1 at 0.001 mM

proof_lines: pH 7.2, 25°C, presence of aflatoxin B1 at 0.1 mM


organism: Mytella charruana

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Homo sapiens

substrate_name: acetyl thiocholine

assay_conditions_summary: pH 8; 300.15 K; buffer 0.1 M Tris

proof_lines: pH 8, 27°C, attachment of polyethylene glycol side chains to lysine residues does not alter the Kcat value

proof_lines: pH 8, 27°C, attachment of polyethylene glycol side chains to lysine residues does not alter the Km value

substrate: acetyl thiocholine


organism: Hoplosternum littorale


organism: Lathyrus oleraceus


organism: Liposcelis entomophila

assay_conditions_summary: pH 7; 310.15 K; buffer 0.1 M phosphate

proof_lines: enzyme from Sichuan Province population, in 0.1 M phosphate buffer (pH 7.0), at 37°C

proof_lines: enzyme from Hubei Province population, in 0.1 M phosphate buffer (pH 7.0), at 37°C

proof_lines: enzyme from Chongqing Municipality population, in 0.1 M phosphate buffer (pH 7.0), at 37°C


organism: Locusta migratoria manilensis

assay_conditions_summary: pH 7.4; 297.15 K; buffer 100 mM phosphate

proof_lines: in 100 mM phosphate buffer, pH 7.4, at 30°C


organism: Lytechinus variegatus


organism: Macroptilium atropurpureum

substrate_name: propionylthiocholine chloride

substrate: propionylthiocholine chloride


organism: Oreochromis mossambicus

proof_lines:
tetrameric enzyme, pH 8, 30°C
tetrameric enzyme, pH 8, 10°C
tetrameric enzyme, pH 8, 20°C


organism: Oreochromis niloticus

proof_lines: in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication


organism: Oryctolagus cuniculus


organism: Ovis aries

proof_lines:
pH 7.4
pH 7.4, presence of cardiotoxin III
pH 7.4, presence of cardiotoxin IV


organism: Parachromis managuensis


organism: Perforatus perforatus


organism: Pollicipes pollicipes


organism: Pseudorasbora parva


organism: Sparus aurata

proof_lines:
pH 7.4, 25°C, enzyme from muscle
pH 7.4, 25°C, enzyme from brain


organism: Tetronarce californica


organism: Zea mays

proof_lines: pH 7.0, 30°C, substrate acetylthiocholine

proof_lines: pH 7.0, 30°C, substrate propionylthiocholine


organism: Sardina pilchardus

proof_lines: enzyme isolated from muscle, 25°C, pH not specified in the publication

proof_lines: enzyme isolated from brain, 25°C, pH not specified in the publication


organism: Schizaphis graminum

proof_lines: pH and temperature not specified in the publication


organism: Scomber scombrus


organism: Sitobion avenae

substrate_name: propylthiocholine

substrate: propylthiocholine


enzyme_display_name: feruloyl esterase

organism: Aspergillus terreus

substrate_name: methyl 4-coumarate

enzyme: feruloyl esterase

substrate: methyl 4-coumarate


organism: Aspergillus usamii

substrate_name: methyl ferulate

substrate: methyl ferulate


organism: Aureobasidium pullulans

substrate_name: methyl sinapate

substrate: methyl sinapate


organism: Auricularia auricula-judae


organism: Acetivibrio thermocellus

substrate_name: ethyl ferulate

substrate: ethyl ferulate


organism: Actinomyces sp.

substrate_name: methyl caffeate

substrate: methyl caffeate


organism: Alternaria tenuissima


organism: Aspergillus awamori

proof_lines: in 20% N,N-dimethyl formamide, 50 mM acetate buffer, pH 5, with 0.05 ml enzyme solution, at 37°C for 10 min


organism: Aspergillus flavus

substrate_name: methyl sinapinate

proof_lines:
pH 5.5, 40°C, recombinant enzyme
pH 6.0, 58°C

substrate: methyl sinapinate


organism: Aspergillus nidulans


organism: Aspergillus ochraceus


organism: Aspergillus oryzae


organism: Bacillus amyloliquefaciens

substrate_name: methyl p-coumarate

substrate: methyl p-coumarate


organism: Bacillus pumilus


organism: Butyrivibrio proteoclasticus

substrate_name: p-nitrophenyl acetate

assay_conditions_summary: pH 7.2; 310.15 K; buffer 100 mM phosphate

proof_lines: in 100 mM phosphate buffer, pH 7.2, at 37°C

substrate: p-nitrophenyl acetate


organism: Caldanaerobacter subterraneus subsp. tengcongensis

assay_conditions_summary: pH 8; 338.15 K; buffer 50 mM sodium phosphate

proof_lines: at 65°C, in 50 mM sodium phosphate buffer, pH 8.0


organism: Rachycentron canadum

substrate_name: acetylthiocholine

substrate: acetylthiocholine


organism: Rattus norvegicus

proof_lines: pH 8, 37°C, brain, membrane-bound form, component I

proof_lines: pH 8, 37°C, erythrocyte, membrane-bound form, component I

proof_lines: pH 8, 37°C, brain, soluble form, component II


organism: Rhopalosiphum padi

substrate_name: butylthiocholine

substrate: butylthiocholine


organism: Rhynchophorus ferrugineus

assay_conditions_summary: pH 8

proof_lines:
isoform AChEIIb, pH 8, 37Â°C
isoform AChEIIIb, pH 8, 37Â°C


organism: Lactarius hatsudake


organism: Lactobacillus acidophilus


organism: Neurospora crassa


organism: Penicillium expansum

substrate_name: methyl coumarate

proof_lines:
p-coumaroyl esterase
37°C, pH 6.0
pH 5.6, 37°C

substrate: methyl coumarate


organism: rumen bacterium

substrate_name: n-decyl ferulate

substrate: n-decyl ferulate


organism: rumen microorganism

proof_lines: transesterification, immobilized enzyme, pH not specified in the publication, 40°C

proof_lines: transesterification, free enzyme, pH not specified in the publication, 40°C

proof_lines: hydrolysis, free enzyme, pH not specified in the publication, 40°C


organism: Russula virescens


organism: Schizophyllum commune


organism: Thermoclostridium stercorarium


organism: Thermothelomyces heterothallicus

proof_lines: transesterification in a n-hexane/t-butanol/water solvent system, 35°C


organism: Thermothelomyces thermophilus


organism: uncultured bacterium


organism: Cellulosilyticum ruminicola


organism: Eleusine coracana

substrate_name: p-nitrophenylferulate

substrate: p-nitrophenylferulate


organism: Escherichia coli

substrate_name: p-coumaric acid methyl ester

substrate: p-coumaric acid methyl ester


organism: Fusarium proliferatum


organism: Sorangium cellulosum

assay_conditions_summary: pH 7

proof_lines: pH 7.0, temperature not specified in the publication


organism: Streptomyces olivochromogenes


organism: Streptomyces sp.


organism: Streptomyces werraensis


organism: Pleurotus eryngii


organism: Pleurotus giganteus


organism: Pleurotus sapidus


organism: Pseudomonas fluorescens


organism: Talaromyces funiculosus


organism: Talaromyces pinophilus

substrate_name: ferulic acid methyl ester

substrate: ferulic acid methyl ester


organism: Talaromyces stipitatus

substrate_name: methyl cinnamate

proof_lines:
hydrolysis
pH 6.0-7.0, 60°C

substrate: methyl cinnamate


organism: Thermochaetoides dissita

substrate_name: 4-nitrophenyl butanoate

substrate: 4-nitrophenyl butanoate


enzyme_display_name: alpha-glucosidase

organism: Entamoeba histolytica

substrate_name: Unknown

proof_lines: versus 4-methylumbelliferyl alpha-D-glucoside, pH 5.5, 45°C

enzyme: alpha-glucosidase

substrate: Unknown


organism: Equus caballus

substrate_name: maltotetraose

substrate: maltotetraose


organism: Erwinia rhapontici


organism: Escherichia coli

substrate_name: maltose

substrate: maltose


organism: Brassica oleracea

proof_lines: and a second alpha-glucosidase with Km of 0.0086 mM

proof_lines: and a second alpha-glucosidase with Km of 0.3 mM


organism: Chilo suppressalis

substrate_name: p-nitrophenyl-alpha-D-glucopyranoside

proof_lines:
salivary gland
midgut

substrate: p-nitrophenyl-alpha-D-glucopyranoside


organism: Drosophila melanogaster

substrate_name: sucrose

proof_lines:
alpha-glucosidase III
alpha-glucosidase II
alpha-glucosidase I

substrate: sucrose


organism: Dysdercus peruvianus


organism: uncultured microorganism

substrate_name: methyl sinapate

proof_lines:
recombinant enzyme, pH 8.0, 37°C
pH 5.0, 30°C

substrate: methyl sinapate


organism: Zea mays

substrate_name: methyl ferulate

substrate: methyl ferulate


organism: Geobacillus stearothermophilus


organism: Geobacillus thermodenitrificans


organism: Glycine max

proof_lines:
alpha-glucoamylase II
glucoamylase I


organism: Homo sapiens

substrate_name: resorufin alpha-D-glucopyranoside

proof_lines: catalytic subunit ntMGAM, pH and temperature not specified in the publication

proof_lines: catalytic subunit ctMGAMN2, pH and temperature not specified in the publication

substrate: resorufin alpha-D-glucopyranoside


organism: Bacillus subtilis

substrate_name: p-nitrophenyl-alpha-D-glucoside

substrate: p-nitrophenyl-alpha-D-glucoside


organism: Bacillus thuringiensis serovar kurstaki

substrate_name: 4-nitrophenyl alpha-D-glucopyranoside

substrate: 4-nitrophenyl alpha-D-glucopyranoside


organism: Beta vulgaris


organism: Bifidobacterium longum subsp. Longum


organism: Aureobasidium pullulans


organism: Bacillus cereus


organism: Bacillus licheniformis

proof_lines: free enzyme, pH not specified in the publication, temperature not specified in the publication

proof_lines: immobilized enzyme entrapped in agar, pH not specified in the publication, temperature not specified in the publication


organism: Bacillus sp. (in: firmicutes)


organism: Apis mellifera

substrate_name: kojibiose

substrate: kojibiose


organism: Aplysia fasciata


organism: Aspergillus niger

substrate_name: maltohexaose

proof_lines:
soluble
DP = 17
intracellular enzyme

substrate: maltohexaose


organism: Aspergillus niveus

substrate_name: p-nitrophenyl alpha-D-glucopyranoside

substrate: p-nitrophenyl alpha-D-glucopyranoside


organism: Oryza sativa

proof_lines:
alpha-glucosidase I
alpha-glucosidase II
G17, alpha-glucosidase I


organism: Paecilomyces variotii


organism: Panicum miliaceum


organism: Parageobacillus thermoglucosidasius


enzyme_display_name: aryldialkylphosphatase

organism: Oryctolagus cuniculus

substrate_name: diethyl paraoxon

assay_conditions_summary: pH 7.4; buffer 20 mM Tris-HCl

proof_lines: in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication

enzyme: aryldialkylphosphatase

substrate: diethyl paraoxon


organism: Mortierella alliacea


organism: Mucor javanicus


organism: Mucor racemosus

substrate_name: maltotriose

substrate: maltotriose


substrate_name: maltopentaose

substrate: maltopentaose


organism: Xanthomonas campestris

substrate_name: Vanillin

substrate: Vanillin


organism: Zea mays

substrate_name: turanose

substrate: turanose


organism: Thermococcus sp.

proof_lines: enzyme immobilized on oxirane acrylic beads


organism: Thermomonospora curvata


organism: Trichoderma viride


organism: Vigna radiata

substrate_name: amylose

proof_lines:
alpha-glucosidase I and II-2
alpha-glucosidase I
alpha-glucosidase II-1

substrate: amylose


organism: Talaromyces purpureogenus

substrate_name: maltose

substrate: maltose


organism: Tetrahymena pyriformis


organism: Thermochaetoides thermophila


organism: Phaffia rhodozyma

substrate_name: soluble starch

substrate: soluble starch


organism: Purpureocillium lilacinum

proof_lines:
pH 5.0, 50°C
soluble substrate, pH 5.0, 50°C


organism: Pyrococcus furiosus

substrate_name: p-nitrophenyl-alpha-D-glucopyranoside

substrate: p-nitrophenyl-alpha-D-glucopyranoside


organism: Quesada gigas

substrate_name: maltoheptaose

substrate: maltoheptaose


organism: Rattus norvegicus


organism: Rhizobium sp.

substrate_name: 4-nitrophenyl alpha-D-glucoside

substrate: 4-nitrophenyl alpha-D-glucoside


organism: Saccharolobus solfataricus


organism: Saccharomyces cerevisiae

proof_lines: pH 7.0, 30°C, recombinant wild-type enzyme


proof_lines:
alpha-glucosidase III
alpha-glucosidase I
alpha-glucosidase II


organism: Saccharomyces pastorianus


organism: Solanum tuberosum


organism: Sus scrofa

proof_lines:
alpha-glucosidase IIa
alpha-glucosidase IIb


enzyme_display_name: beta-glucosidase

organism: Daldinia eschscholtzii

proof_lines: pH and temperature not specified in the publication

enzyme: beta-glucosidase


organism: Hanseniaspora uvarum

substrate_name: 4-nitrophenyl beta-D-glucoside

substrate: 4-nitrophenyl beta-D-glucoside


enzyme_display_name: funoran endo-beta-hydrolase

organism: Wenyingzhuangia aestuarii

substrate_name: funoran

enzyme: funoran endo-beta-hydrolase

substrate: funoran


organism: Hordeum vulgare


organism: Lactobacillus acidophilus

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Lathyrus oleraceus

proof_lines:
alpha-glucosidase 1
alpha-glucosidase 2
alpha-glucosidase 3


organism: Levilactobacillus brevis


enzyme_display_name: peptidyl-dipeptidase A

organism: Alitta virens

substrate_name: benzoyl-Gly-His-Leu

enzyme: peptidyl-dipeptidase A

substrate: benzoyl-Gly-His-Leu


organism: Bacillus pumilus


organism: Bos taurus

proof_lines:
pH 7.5, 25°C, N-domain from lung enzyme
pH 7.5, 25°C, enzyme from lung
pH 7.5, 25°C, enzyme from testis


organism: Cavia porcellus

substrate_name: angiotensin I

proof_lines:
lung enzyme
serum enzyme

substrate: angiotensin I


organism: Oryctolagus cuniculus

assay_conditions_summary: pH 7.5; 310.15 K; buffer 50 mM Tris-HCl

proof_lines:
enzyme from lung
enzyme from testis
enzyme from testis and lung


organism: Pseudomonas sp.

substrate_name: Unknown

substrate: Unknown


organism: Rattus norvegicus

proof_lines: pH 7.5, 37°C, enzyme from brain, 200 mM NaCl

proof_lines: pH 7.5, 37°C, enzyme from brain, 10 mM NaCl

proof_lines: pH 7.5, 37°C, enzyme from testis, 10 mM NaCl


organism: Struthio camelus


organism: Drosophila sp. (in: flies)

proof_lines:
35°C, pH 7.0, 0 mM NaCl
35°C, pH 8.0, 0 mM NaCl
35°C, pH 8.0, 100 mM NaCl


organism: Gallus gallus

substrate_name: hippuryl-His-Leu

proof_lines:
detergent-solubilized enzyme
trypsin-extracted enzyme

substrate: hippuryl-His-Leu


organism: Homo sapiens

proof_lines: 37°C, pH 7.0, full-length mutant containing only a functional N-domain catalytic site

proof_lines: 37°C, pH 7.0, full-length mutant containing only a functional C-domain catalytic site


organism: Locusta migratoria

substrate_name: NKLKPSQWISL

substrate: NKLKPSQWISL


organism: Sus scrofa

proof_lines:
pH 7.5, 25°C
pH 7.5, 25°C, cobalt-ACE
pH 6, 0.1 M NaCl


organism: Ucides cordatus

substrate_name: Abz-LFK(Dnp)-OH

substrate: Abz-LFK(Dnp)-OH


enzyme_display_name: chymotrypsin

substrate_name: benzoyl-Gly-4-guanidinophenyl ester

assay_conditions_summary: pH 7.6; 298.15 K; buffer saline, pH 7

proof_lines:
25°C, pH 7.6
transesterfication in 10% ethanol
21°C, pH 8.3, 20 mM CaCl2

enzyme: chymotrypsin

substrate: benzoyl-Gly-4-guanidinophenyl ester


organism: Camelus bactrianus

substrate_name: acetyl-Trp ethyl ester

substrate: acetyl-Trp ethyl ester


organism: Camelus dromedarius

substrate_name: N-acetyl-L-leucine methyl ester

substrate: N-acetyl-L-leucine methyl ester


organism: Carassius auratus

proof_lines:
chymotrypsin B, at pH 7.5 and 37°C
chymotrypsin A, at pH 7.5 and 37°C


organism: Diatraea saccharalis


organism: Gadus morhua

substrate_name: N-benzoyl-Tyr p-nitroanilide

proof_lines: enzyme form A and enzyme form B, at pH 10°C

substrate: N-benzoyl-Tyr p-nitroanilide


organism: Musca domestica

proof_lines: pH 8.5, temperature not specified in the publication


organism: Panulirus interruptus


organism: Pterygoplichthys disjunctivus


organism: Sardinops caeruleus

substrate_name: succinyl-Ala-Ala-Pro-Phe-p-nitroanilide

proof_lines:
at 30°C, chymotrypsin II
at 30°C, chymotrypsin I

substrate: succinyl-Ala-Ala-Pro-Phe-p-nitroanilide


organism: Sardinops sagax

substrate_name: N-succinyl-L-Ala-Ala-Pro-p-nitroanilide

proof_lines: in 50 mM Tris-HCl pH 8.0 with 10 mM CaCl2, at 30 °C

substrate: N-succinyl-L-Ala-Ala-Pro-p-nitroanilide


organism: Sepia officinalis


organism: Struthio camelus


organism: synthetic construct

assay_conditions_summary: 310.15 K; buffer 50 mM Tris-HCl

proof_lines: at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl


organism: Tenebrio molitor


enzyme_display_name: tissue kallikrein

organism: Homo sapiens

substrate_name: Abz-KLFSSEQ-EDDnp

assay_conditions_summary: pH 7.5; 310.15 K; buffer 20 mM Tris/HCl

proof_lines:
at pH 7.5 and 37°C
pH 9.0, 30°C
pH 9.0, 37°C

enzyme: tissue kallikrein

substrate: Abz-KLFSSEQ-EDDnp


organism: Mus musculus


organism: Rattus norvegicus


organism: Sus scrofa

substrate_name: N-alpha-tosyl-L-arginine methyl ester

proof_lines:
alpha-kallikrein, pH 8.0, 25°C
pH 9.0, 25°C
pH 9.0, 30°C

substrate: N-alpha-tosyl-L-arginine methyl ester


enzyme_display_name: furin

organism: Saccharomyces cerevisiae

enzyme: furin


enzyme_display_name: trypsin

organism: Bos taurus

substrate_name: M-diarginyl insulin

assay_conditions_summary: pH 8; 310.15 K; buffer 67 mM sodium phosphate

proof_lines:
pH 8.0, 37°C
at 5°C
gamma-trypsin

enzyme: trypsin

substrate: M-diarginyl insulin


organism: Diatraea saccharalis

proof_lines: at 30°C in 100 mM Tris-HCl buffer pH 8.5


proof_lines:
pH 8.0, 37°C, wild-type enzyme
wild-type enzyme, pH 9
wild-type enzyme, pH 8


substrate_name: FITC-casein

substrate: FITC-casein


enzyme_display_name: cathepsin L

organism: Fasciola gigantica

proof_lines: wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C

enzyme: cathepsin L


organism: Gallus gallus


assay_conditions_summary: pH 5.5; 310.15 K; buffer 50 mM Mes

proof_lines: 50 mM Na-acetate, 100 mM NaCl, 2.5 mM EDTA, pH 5.5, 37°C

proof_lines: in 50 mM Mes buffer (pH 6.0) containing 5 mM dithiothreitol, at 37°C

proof_lines: recombinant enzyme expressed Escherichia coli


organism: Hypophthalmichthys molitrix


enzyme_display_name: ribulose-bisphosphate carboxylase

organism: Agave victoriae-reginae

substrate_name: D-ribulose 1,5-bisphosphate

proof_lines: in 100 mM Bicine-NaOH pH 8.0, 20 mM MgCl2, at 25°C

enzyme: ribulose-bisphosphate carboxylase

substrate: D-ribulose 1,5-bisphosphate


organism: Helianthus annuus


organism: Nicotiana tabacum

proof_lines: enzyme assembled with small subunit isoform RbcS-S1, pH 8.0, 25°C

proof_lines: enzyme assembled with small subunit isoform RbcS-S5, pH 8.0, 25°C

proof_lines: enzyme assembled with small subunit isoform RbcS-S2, pH 8.0, 25°C


organism: Pseudomonas sp.

proof_lines: recombinant mutant H57betaS/H70betaS/M165alphaS/I176betaS, pH 8.0, 37°C

proof_lines: recombinant mutant H57betaS/H70betaS/L176betaK, pH 8.0, 37°C

proof_lines: recombinant mutant H57betaS/H70betaS/F58betaD, pH 8.0, 37°C


organism: Pseudomonas sp. 130

substrate_name: glutaryl-7-aminocephalosporanic acid

substrate: glutaryl-7-aminocephalosporanic acid


organism: Spodoptera frugiperda

proof_lines: 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C


organism: Takifugu rubripes


organism: Toxoplasma gondii

substrate_name: Benzyloxycarbonyl-Lys-Arg

substrate: Benzyloxycarbonyl-Lys-Arg


organism: Trypanosoma brucei

proof_lines: in the presence of 0.001 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C

proof_lines: in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 37°C


organism: Brevibacillus laterosporus


organism: Brevundimonas diminuta


organism: Pseudomonas nitroreducens

substrate_name: L-gamma-glutamyl p-nitroanilide

substrate: L-gamma-glutamyl p-nitroanilide


substrate_name: glutaryl-GGGGGK

proof_lines: at 37°C, pH not specified in the publication

substrate: glutaryl-GGGGGK


organism: Agama stellio stellio


organism: Apostichopus japonicus


organism: Cavia porcellus


organism: Cyprinus carpio


organism: Mus musculus

substrate_name: Unknown

substrate: Unknown


organism: Oncorhynchus keta


organism: Oryctolagus cuniculus


organism: Rattus norvegicus


organism: Cryptosporidium parvum


organism: Homo sapiens

substrate_name: pro-MT-MMP 3 protein

proof_lines:
pH 7.0, 37°C
pH 7.0, 37°C, presence of Mg2+
pH 7.0, 37°C, absence of Mg2+

substrate: pro-MT-MMP 3 protein


organism: Mammalia


enzyme_display_name: catechol oxidase

organism: Solanum tuberosum

substrate_name: catechol

enzyme: catechol oxidase

substrate: catechol


enzyme_display_name: ubiquinol oxidase (non-electrogenic)

organism: Trypanosoma brucei brucei

verification_status: unverified

source_license: CC BY 4.0; CC BY-NC-ND 4.0

assay_conditions_summary: pH 7.5; 298.15 K; buffer conditions

proof_lines:
pH 7.5, 25°C
pH 7.4, 25°C
Raw SKiD Env: Temp=25°C, pH=7.5

source_databases_merged: brenda skid

enzyme: ubiquinol oxidase (non-electrogenic)

status: unverified


organism: Marinomonas mediterranea

substrate_name: Syringaldazine; O2

sequence_source: UniProtKB direct accession lookup Q9KJB8 via rest.uniprot.org/uniprotkb/Q9KJB8.json on 2026-06-05

substrate: Syringaldazine; O2


organism: Prunus armeniaca

substrate_name: O2; Catechol

sequence_source: UniProtKB direct accession lookup O81103 via rest.uniprot.org/uniprotkb/O81103.json on 2026-06-05

substrate: O2; Catechol


enzyme_display_name: laccase

organism: Bacillus amyloliquefaciens

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

enzyme: laccase


organism: Bacillus altitudinis


enzyme_display_name: grixazone synthase

organism: Streptomyces griseus

enzyme: grixazone synthase


organism: Acinetobacter baumannii

substrate_name: O2; Guaiacol

substrate: O2; Guaiacol


organism: Bacillus licheniformis


source_db: oed

source: oed


organism: Bacillus atrophaeus


substrate_name: Syringaldazine

substrate: Syringaldazine


organism: Bacillus pumilus


source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)


substrate_name: O2; Syringaldazine

substrate: O2; Syringaldazine


organism: Bacillus sp. HR03


organism: Bacillus subtilis


sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


substrate_name: Guaiacol; O2

substrate: Guaiacol; O2


organism: Bacteroides thetaiotaomicron


organism: Bacillus tequilensis


organism: Burkholderia cepacia


organism: Cerrena unicolor

source_db: uniprot

source: uniprot


substrate_name: syringaldazine

verification_status: verified

sequence: AIGPVADLHI

wild_type_sequence: AIGPVADLHI

substrate: syringaldazine

status: verified


substrate_name: ABTS (at 70 degrees Celsius)

substrate: ABTS (at 70 degrees Celsius)


organism: Campylobacter jejuni

sequence_source: Selected-batch preserved UniProtKB REST accession A0A0H3PBA4 evidence, with RCSB 3ZX1 and PMID 22127520 cross-check retained in the shared family context.


organism: Cyathus bulleri

substrate_name: guaiacol

substrate: guaiacol


organism: Escherichia coli

substrate_name: 2,6-Dimethoxyphenol; O2

substrate: 2,6-Dimethoxyphenol; O2


organism: Fomitopsis pinicola


sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant P444I


sequence_source: Selected-batch preserved canonical context: UniProtKB:S8FIE4 direct FASTA; reviewed Swiss-Prot laccase EC 1.10.3.2; PMID:34116754 reference.


organism: Galerina sp

substrate_name: ABTS

sequence: GPSTDLVIGNKGFDGGIDSAILR

wild_type_sequence: GPSTDLVIGNKGFDGGIDSAILR

substrate: ABTS


organism: Ganoderma lucidum


organism: Galerina sp. DSM 22662

substrate_name: O2; Syringic acid

substrate: O2; Syringic acid


organism: Klebsiella pneumoniae


organism: Lepiota magnispora

substrate_name: ABTS (at pH 4)

sequence: VTIGKEGTLT

wild_type_sequence: VTIGKEGTLT

assay_conditions_summary: pH 4

substrate: ABTS (at pH 4)


organism: Panus rudis


organism: Ochrobactrum sp.

sequence_source: UniProtKB exact accession A6X5N0 REST lookup, retrieved 2026-07-01


organism: Podospora anserina


organism: Pseudomonas putida


organism: Pleurotus ostreatus


organism: Pseudomonas sp.


sequence_source: UniProtKB exact accession Q12739 REST lookup, retrieved 2026-07-01


organism: Rhizobium meliloti


organism: Rhodobacter capsulatus

substrate_name: O2; 2,6-Dimethoxyphenol

substrate: O2; 2,6-Dimethoxyphenol


organism: Salmonella typhimurium


sequence_source: UniProtKB exact accession Q8ZRS2 REST lookup, retrieved 2026-07-01


organism: Stenotrophomonas maltophilia


organism: Streptomyces coelicolor


organism: Staphylococcus haemolyticus

sequence_source: UniProtKB REST direct lookup L7SV51 on 2026-05-30


organism: Streptomyces ipomoeae

sequence_source: UniProtKB REST canonical sequence for A3QVH2


organism: Streptomyces sp. C1

substrate_name: L-Dopa; O2

substrate: L-Dopa; O2


organism: Thermothelomyces thermophilus


organism: Streptomyces viridochromogenes

sequence_source: UniProtKB REST canonical sequence for J9S5G3


organism: Toxicodendron vernicifluum

substrate_name: ferrocyanide

substrate: ferrocyanide


organism: Thermus thermophilus

sequence_source: UniProtKB:F6DF14 FASTA; direct REST lookup on 2026-05-30


organism: Trametes cinnabarina

sequence_source: UniProtKB Q9UVQ2 REST canonical sequence


organism: Trametes sp. C30


sequence_source: UniProtKB:Q8NID5 direct lookup


organism: unknown prokaryotic organism


organism: Homo sapiens

substrate_name: NRH

assay_conditions_summary: cofactor NADH

substrate: NRH


enzyme_display_name: L-ascorbate peroxidase

organism: Citrus limon

smiles: OO

enzyme: L-ascorbate peroxidase


substrate_name: Syringaldazine; O2

substrate: Syringaldazine; O2


substrate_name: L-ascorbate

substrate: L-ascorbate


enzyme_display_name: o-aminophenol oxidase

organism: Streptomyces antibioticus

substrate_name: dioxygen

enzyme: o-aminophenol oxidase

substrate: dioxygen


organism: Crocus sativus


organism: Trametes versicolor

source_db: skid

source_license: CC BY-NC-ND 4.0

proof_lines:
Raw SKiD Env: Temp=30°C, pH=3
Raw SKiD Env: Temp=30°C, pH=3.4

source: skid


organism: Pisum sativum

substrate_name: L-Ascorbate

substrate: L-Ascorbate


substrate_name: H2O2; L-Ascorbate

substrate: H2O2; L-Ascorbate


organism: Solanum lycopersicum

substrate_name: cumene hydroperoxide

substrate: cumene hydroperoxide


organism: Mus musculus

substrate_name: H(2)O(2) (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0)

assay_conditions_summary: pH 7; buffer 0.1 M phosphate


enzyme_display_name: manganese peroxidase

organism: Moniliophthora roreri

substrate_name: veratryl alcohol

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

enzyme: manganese peroxidase

substrate: veratryl alcohol


organism: Cerrena unicolor

sequence_source: UniProt REST A0A7D5FUQ6 FASTA


substrate_name: Reactive Black 5

substrate: Reactive Black 5


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature A172W/A273T with inferred residue-numbering offset +19 to reconstruct the assayed variant sequence.


organism: Trypanosoma cruzi


sequence_variant_note: Resolved canonical sequence from UniProt V2XS39 FASTA, reconstructed with paper-to-UniProt residue offset +19 and applied mutation signature A172W/K168V with inferred residue-numbering offset +19 to reconstruct the assayed variant sequence.


organism: Phanerochaete chrysosporium

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R177E with inferred residue-numbering offset +21 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: manganese peroxidase(Enzyme) mutant R177E


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R177N with inferred residue-numbering offset +21 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: manganese peroxidase(Enzyme) mutant R177N


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R177D with inferred residue-numbering offset +21 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: manganese peroxidase(Enzyme) mutant R177D


enzyme_display_name: lignin peroxidase

organism: Phanerodontia chrysosporium

enzyme: lignin peroxidase


sequence: AVXHPGTXVSXAAXXXFIPL

wild_type_sequence: AVXHPGTXVSXAAXXXFIPL


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R177Q with inferred residue-numbering offset +21 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: manganese peroxidase(Enzyme) mutant R177Q


smiles: [Ho]


enzyme_display_name: versatile peroxidase

organism: Pleurotus eryngii

enzyme: versatile peroxidase


sequence_source: UniProtKB:P11542 REST FASTA, fetched 2026-06-07


organism: Fasciola hepatica

substrate_name: t-Butylhydroperoxide

smiles: CC(C)(C)OO

substrate: t-Butylhydroperoxide


substrate_name: guaiacol

substrate: guaiacol


enzyme_display_name: dye decolorizing peroxidase

organism: Bacillus subtilis

enzyme: dye decolorizing peroxidase


organism: Irpex lacteus

sequence_source: UniProtKB:A0A2P1C6N4 plus RCSB 7D8M identity check


organism: Mycetinis scorodonius


enzyme_display_name: bromide peroxidase

organism: Corallina officinalis

substrate_name: bromide

enzyme: bromide peroxidase

substrate: bromide


organism: Pleurotus sapidus

proof_lines:
at pH 3.5 and 30°C
at pH 4.0 and 30°C


organism: Vibrio cholerae


enzyme_display_name: thioredoxin-dependent peroxiredoxin

organism: Aeropyrum pernix

sequence_source: UniProtKB:Q9Y9L0 REST lookup, release 2026_02

enzyme: thioredoxin-dependent peroxiredoxin


organism: Escherichia coli (strain K12)

substrate_name: H(2)O(2) (using glutathione Grx1 as electron donor)


organism: Escherichia coli

substrate_name: linoleic acid hydroperoxide

sequence_source: UniProtKB:P0AE52 REST lookup, release 2026_02

smiles: CCCCCC=CCC=CCCCCCCCC(=O)OO

substrate: linoleic acid hydroperoxide


enzyme_display_name: hydroperoxy fatty acid reductase

organism: Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

enzyme: hydroperoxy fatty acid reductase

substrate: NADPH


organism: Rhizobium etli


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)


substrate_name: H(2)O(2) (using glutathione as electron donor)


substrate: H(2)O(2) (using glutathione as electron donor)


enzyme_display_name: glutathione-dependent peroxiredoxin

organism: Bos taurus

enzyme: glutathione-dependent peroxiredoxin


enzyme_display_name: NADH-dependent peroxiredoxin

organism: Amphibacillus xylanus (strain ATCC 51415 / DSM 6626 / JCM 7361 / LMG 17667 / NBRC 15112 / Ep01)

enzyme: NADH-dependent peroxiredoxin


organism: Treponema pallidum

assay_conditions_summary: pH 7; 298.15 K; cofactor NADH


organism: Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)


organism: Haemophilus influenzae

proof_lines:
pH 7.8, 25°C
Raw SKiD Env: Temp=25°C, pH=7.8


organism: Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)


enzyme_display_name: lipoyl-dependent peroxiredoxin

organism: Xylella fastidiosa

enzyme: lipoyl-dependent peroxiredoxin


organism: Rattus norvegicus

substrate_name: dipalmitoyl phosphatidylcholine

substrate: dipalmitoyl phosphatidylcholine


enzyme_display_name: cytochrome-c peroxidase

organism: Nitrosomonas europaea

verification_status: corrected

enzyme: cytochrome-c peroxidase

status: corrected


enzyme_display_name: catalase

organism: Bacillus altitudinis

sequence_source: Selected-batch citation: UniProt REST uniprotkb/W6AYH3.json, accessed 2026-06-05; current accession check found the entry deleted.

enzyme: catalase


organism: Candida tropicalis


organism: Bacteroides fragilis


organism: Listeria seeligeri


organism: Homo sapiens


organism: Bordetella pertussis


organism: Geobacillus sp. B1


organism: Micrococcus luteus


organism: Proteus mirabilis


organism: Ruegeria pomeroyi DSS-3


organism: Pteris vittata

assay_conditions_summary: pH 7; buffer 50 mM potassium phosphate

proof_lines: in 50 mM potassium phosphate buffer (pH 7.0)


organism: Serratia marcescens

assay_conditions_summary: pH 7; 293.15 K; buffer 50 mM NaH2PO4-Na2HPO4

proof_lines: in 50 mM NaH2PO4-Na2HPO4 buffer (pH 7.0) at 20°C


enzyme_display_name: peroxidase

organism: Allium sativum

enzyme: peroxidase


organism: Synechocystis sp.


organism: Amycolatopsis sp.

substrate_name: H2O2; 2,4-Dichlorophenol

substrate: H2O2; 2,4-Dichlorophenol


organism: Armoracia rusticana

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

proof_lines:
at pH 6.0
Raw SKiD Env: Temp=-----, pH=6
Raw SKiD Env: Temp=30°C, pH=6.5

source_databases_merged: sabio_rk brenda skid


organism: Equus caballus


organism: Bos taurus

substrate_name: ethynylestradiol

proof_lines:
at 25°C, pH 6.0
at pH 6.0 and 22°C

substrate: ethynylestradiol


organism: Escherichia coli


organism: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)

substrate_name: lauric acid

substrate: lauric acid


enzyme_display_name: glutathione peroxidase

enzyme: glutathione peroxidase


organism: Saccharomyces cerevisiae

substrate_name: H2O2; Reduced glutathione

substrate: H2O2; Reduced glutathione


organism: Homo sapiens

substrate_name: glutathione

substrate: glutathione


organism: Mus musculus

assay_conditions_summary: pH 7; 298.15 K; buffer phosphate

substrate: H(2)O(2) (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0)


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)


enzyme_display_name: tryptophan 2,3-dioxygenase

substrate_name: L-1-Methyltryptophan; O2

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature H76S to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: tryptophan 2,3-dioxygenase(Enzyme) mutant TDO H76S, C-terminal His6-tag

enzyme: tryptophan 2,3-dioxygenase

substrate: L-1-Methyltryptophan; O2


substrate_name: O2; L-Tryptophan

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature Y45A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: tryptophan 2,3-dioxygenase(Enzyme) mutant Y45A (aa 19-388)

substrate: O2; L-Tryptophan


substrate_name: L-Tryptophan; O2

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R144A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: tryptophan 2,3-dioxygenase(Enzyme) mutant R144A (aa 19-388)

substrate: L-Tryptophan; O2


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S151A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: tryptophan 2,3-dioxygenase(Enzyme) mutant S151A (aa 19-388)


organism: Cupriavidus metallidurans


substrate_name: L-Tryptophan

substrate: L-Tryptophan


enzyme_display_name: glutaredoxin-dependent peroxiredoxin

enzyme: glutaredoxin-dependent peroxiredoxin


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature F140A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: tryptophan 2,3-dioxygenase(Enzyme) mutant F140A (aa 19-388)


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

substrate_name: H(2)O(2) (in strain FRD1)

substrate: H(2)O(2) (in strain FRD1)


organism: Trypanosoma cruzi

substrate_name: linolenic acid hydroperoxide

substrate: linolenic acid hydroperoxide


organism: Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

substrate_name: L-tryptophan

substrate: L-tryptophan


enzyme_display_name: linoleate 13S-lipoxygenase

organism: Triticum turgidum subsp. durum

substrate_name: arachidonic acid

sequence_source: UniProtKB:A0A7M3TWE5 direct REST lookup on 2026-06-07

enzyme: linoleate 13S-lipoxygenase

substrate: arachidonic acid


organism: Olea europaea

substrate_name: linolenic acid

sequence_source: UniProtKB:B6D1W5 direct REST lookup on 2026-06-07

substrate: linolenic acid


organism: Myxococcus xanthus


organism: Sus scrofa

smiles: CCCCCC=CCC=CCC=CCC=CCCCC(=O)[O-]


enzyme_display_name: 4-hydroxyphenylpyruvate dioxygenase

organism: Arabidopsis thaliana

substrate_name: O2; 4-Hydroxyphenylpyruvate

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

enzyme: 4-hydroxyphenylpyruvate dioxygenase

substrate: O2; 4-Hydroxyphenylpyruvate


enzyme_display_name: quercetin 2,3-dioxygenase

organism: Bacillus subtilis

substrate_name: Quercetin; O2

sequence_source: UniProtKB:P42106 direct REST lookup on 2026-06-15

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

enzyme: quercetin 2,3-dioxygenase

substrate: Quercetin; O2


organism: Frog


organism: Daucus carota

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


organism: Mycobacterium tuberculosis


organism: Pseudomonas sp.

substrate_name: phenylpyruvate

substrate: phenylpyruvate


organism: Rattus norvegicus


organism: Homo sapiens


enzyme_display_name: 1-hydroxy-2-naphthoate 1,2-dioxygenase

organism: Nocardioides sp.

enzyme: 1-hydroxy-2-naphthoate 1,2-dioxygenase


organism: Streptomyces avermitilis


enzyme_display_name: biphenyl-2,3-diol 1,2-dioxygenase

organism: Achromobacter sp.

enzyme: biphenyl-2,3-diol 1,2-dioxygenase


sequence_source: UniProtKB:B3VTL5 direct lookup; live TrEMBL entry B3VTL5_9BURK; sequence version 1; entry version 33; accessed 2026-06-06


organism: Bacillus sp. (in: firmicutes)

substrate_name: catechol

sequence_source: UniProtKB:Q8GR45 direct lookup; reviewed Swiss-Prot entry BPHC_GEOG3; sequence version 1; entry version 68; accessed 2026-06-06

substrate: catechol


organism: Geobacillus genomosp. 3

substrate_name: 2,3-dihydroxybiphenyl (at 60 degrees Celsius and pH 7.5)


enzyme_display_name: gentisate 1,2-dioxygenase

organism: Klebsiella pneumoniae

substrate_name: gentisate

enzyme: gentisate 1,2-dioxygenase

substrate: gentisate


organism: Martelella sp. AD-3


organism: Rhizorhabdus dicambivorans


organism: Ralstonia sp.

substrate_name: O2; Gentisate

substrate: O2; Gentisate


enzyme_display_name: arachidonate 8-lipoxygenase

organism: Plexaura homomalla

substrate_name: arachidonate

smiles: CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(=O)O

enzyme: arachidonate 8-lipoxygenase

substrate: arachidonate


organism: Sphingomonas sp.


enzyme_display_name: linoleate 11-lipoxygenase

organism: Gaeumannomyces graminis var. avenae

substrate_name: O2; alpha-Linolenate

sequence_source: UniProtKB reviewed accession Q8X151

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

enzyme: linoleate 11-lipoxygenase

substrate: O2; alpha-Linolenate


enzyme_display_name: 1,2-dihydroxynaphthalene dioxygenase

organism: Pseudomonas putida

substrate_name: 3-methylcatechol (at pH 5.5)

enzyme: 1,2-dihydroxynaphthalene dioxygenase

substrate: 3-methylcatechol (at pH 5.5)


enzyme_display_name: gallate dioxygenase

organism: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)

substrate_name: gallate

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

enzyme: gallate dioxygenase

substrate: gallate


enzyme_display_name: linoleate 9S-lipoxygenase

organism: Solanum lycopersicum

substrate_name: linoleate

sequence_source: UniProtKB P38415 direct accession lookup

smiles: CCCCCC=CCC=CCCCCCCCC(=O)[O-]

enzyme: linoleate 9S-lipoxygenase

substrate: linoleate


enzyme_display_name: 3-hydroxyanthranilate 3,4-dioxygenase

organism: Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

enzyme: 3-hydroxyanthranilate 3,4-dioxygenase


organism: Rattus norvegicus


organism: Sphingobium xenophagum

sequence_source: UniProtKB:P74836 reviewed Swiss-Prot entry NSAC_SPHXE, cross-checked against PMID 2050635, BRENDA EC 1.13.11.56 cross-reference, and BN6 strain evidence.


organism: Capsicum annuum

sequence_source: UniProtKB D3TTH9 direct accession lookup (canonical sequence already preserved in the selected batch)


enzyme_display_name: linolenate 9R-lipoxygenase

organism: Nostoc sp. PCC 7120 = FACHB-418

substrate_name: alpha-linolenic acid

enzyme: linolenate 9R-lipoxygenase

substrate: alpha-linolenic acid


enzyme_display_name: beta-carotene 15,15'-dioxygenase

organism: Mus musculus

substrate_name: beta-Carotene; O2

sequence_source: UniProtKB reviewed accession Q9JJS6 fetched by direct UniProt REST check on 2026-06-11

enzyme: beta-carotene 15,15'-dioxygenase

substrate: beta-Carotene; O2


organism: Candidatus Nitrosotalea devanaterra


enzyme_display_name: 2-aminophenol 1,6-dioxygenase

organism: Comamonas testosteroni

assay_conditions_summary: pH 8; 295.15 K; buffer 10 mM sodium phosphate

proof_lines: in 10 mM sodium phosphate buffer, at pH 8.0 and 22°C

enzyme: 2-aminophenol 1,6-dioxygenase


enzyme_display_name: linoleate 8R-lipoxygenase

organism: Aspergillus nidulans

substrate_name: palmitoleate

sequence_source: EBI Proteins API UniProt accession Q6RET3

smiles: CCCCCCC=CCCCCCCCC(=O)[O-]

enzyme: linoleate 8R-lipoxygenase

substrate: palmitoleate


organism: Nitrosotalea devaniterrae


organism: Pseudomonas sp.

substrate_name: 2-Aminophenol; O2

substrate: 2-Aminophenol; O2


enzyme_display_name: 2-hydroxyethylphosphonate dioxygenase

organism: Streptomyces viridochromogenes

sequence_source: UniProt REST direct accession lookup, Q5IW40, 2026-06-15

smiles: C(CP(=O)(O)O)O

enzyme: 2-hydroxyethylphosphonate dioxygenase


organism: Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)


organism: uncultured bacterium HF130_AEPn_1

sequence_source: UniProtKB D0E8I5 canonical sequence, already preserved in the selected batch.


enzyme_display_name: protocatechuate 4,5-dioxygenase

organism: Sphingobium sp.

substrate_name: protocatechuate

enzyme: protocatechuate 4,5-dioxygenase

substrate: protocatechuate


enzyme_display_name: oleate 10S-lipoxygenase

substrate_name: oleate

assay_conditions_summary: pH 9; 303.15 K; buffer paper note 'in 0.2 M sodium borate

proof_lines: in 0.2 M sodium borate buffer, pH 9.0, at 30°C

enzyme: oleate 10S-lipoxygenase

substrate: oleate


organism: Novosphingobium aromaticivorans


enzyme_display_name: nitronate monooxygenase

organism: Metarhizium brunneum

sequence_source: UniProtKB direct accession check: A0A6C0WVK2 unreviewed TrEMBL entry A0A6C0WVK2_9HYPO

enzyme: nitronate monooxygenase


enzyme_display_name: Oplophorus-luciferin 2-monooxygenase

organism: Oplophorus gracilirostris

substrate_name: coelenterazine

enzyme: Oplophorus-luciferin 2-monooxygenase

substrate: coelenterazine


sequence_source: UniProtKB direct accession check: A0A6C0WVH0 unreviewed TrEMBL entry A0A6C0WVH0_9HYPO


organism: Pseudomonas syringae

proof_lines:
pH 8.0, 25°C
Raw SKiD Env: Temp=25°C, pH=8


enzyme_display_name: catalase

organism: Brucella abortus

enzyme: catalase


smiles: O=C(O)CCC(=O)C(=O)O


enzyme_display_name: firefly luciferase

organism: Amydetes vivianii

substrate_name: D-firefly luciferin

enzyme: firefly luciferase

substrate: D-firefly luciferin


organism: Luciola cruciata

substrate_name: lauric acid

substrate: lauric acid


enzyme_display_name: inositol oxygenase

organism: Sporopachydermia lactativora

smiles: O=O

enzyme: inositol oxygenase


organism: Rattus norvegicus

substrate_name: Inositol

substrate: Inositol


enzyme_display_name: phenylalanine 2-monooxygenase

substrate_name: L-phenylalanine

sequence_source: UniProtKB Q5W9R9 direct REST lookup; reviewed Swiss-Prot entry PAO_PSESP

assay_conditions_summary: pH 7; 303.15 K; buffer 50 mM potassium phosphate

enzyme: phenylalanine 2-monooxygenase

substrate: L-phenylalanine


enzyme_display_name: gamma-butyrobetaine dioxygenase

enzyme: gamma-butyrobetaine dioxygenase


organism: Pseudomonas sp

substrate_name: L-Phe (for the oxygenation reaction)

substrate: L-Phe (for the oxygenation reaction)


enzyme_display_name: deacetoxyvindoline 4-hydroxylase

organism: Catharanthus roseus

enzyme: deacetoxyvindoline 4-hydroxylase


enzyme_display_name: deacetoxycephalosporin-C hydroxylase

organism: Streptomyces clavuligerus

substrate_name: cephalosporin G

enzyme: deacetoxycephalosporin-C hydroxylase

substrate: cephalosporin G


organism: Hapsidospora chrysogena


enzyme_display_name: procollagen-lysine 5-dioxygenase

organism: Gallus gallus

enzyme: procollagen-lysine 5-dioxygenase


enzyme_display_name: L-asparagine oxygenase

organism: Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

substrate_name: L-asparagine

enzyme: L-asparagine oxygenase

substrate: L-asparagine


enzyme_display_name: thymine dioxygenase

organism: Neurospora crassa

enzyme: thymine dioxygenase


enzyme_display_name: feruloyl-CoA 6-hydroxylase

organism: Arabidopsis thaliana

substrate_name: trans-feruloyl-CoA

assay_conditions_summary: pH 6.5; 303.15 K; cofactor CoA

enzyme: feruloyl-CoA 6-hydroxylase

substrate: trans-feruloyl-CoA


organism: Ruta graveolens

assay_conditions_summary: pH 7.2; 303.15 K; cofactor CoA


organism: Zea mays

assay_conditions_summary: pH 7; 303.15 K; buffer 100 mM potassium phosphate

proof_lines: in 100 mM potassium phosphate buffer (pH 7.0), at 30°C


enzyme_display_name: trans-4-coumaroyl-CoA 2'-hydroxylase

enzyme: trans-4-coumaroyl-CoA 2'-hydroxylase


enzyme_display_name: trimethyllysine dioxygenase

enzyme: trimethyllysine dioxygenase


substrate_name: trimethyllysine

smiles: C[N+](C)(C)CCCCC(C(=O)O)N

substrate: trimethyllysine


organism: Bos taurus

substrate_name: alpha-ketoglutarate

substrate: alpha-ketoglutarate


enzyme_display_name: alkyl sulfatase

organism: Pseudomonas putida

substrate_name: nonyl sulfate

proof_lines:
pH 7, 30°C
Raw SKiD Env: Temp=30°C, pH=7

enzyme: alkyl sulfatase

substrate: nonyl sulfate


enzyme_display_name: flavanone 3-dioxygenase

organism: x Ascocenda

substrate_name: naringenin

enzyme: flavanone 3-dioxygenase

substrate: naringenin


organism: Albuca bracteata


enzyme_display_name: methylphosphonate hydroxylase

organism: Gimesia maris

substrate_name: methylphosphonate

enzyme: methylphosphonate hydroxylase

substrate: methylphosphonate


enzyme_display_name: terephthalate 1,2-dioxygenase

organism: Comamonas sp

substrate_name: NADP (at 30 degrees Celsius and at ph 7)

assay_conditions_summary: pH 7; cofactor NADPH

enzyme: terephthalate 1,2-dioxygenase


enzyme_display_name: pyrrole-2-carboxylate monooxygenase

organism: Rhodococcus sp. (in: high G+C Gram-positive bacteria)

substrate_name: NADH

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADH

enzyme: pyrrole-2-carboxylate monooxygenase

substrate: NADH


enzyme_display_name: 2-aminobenzenesulfonate 2,3-dioxygenase

organism: Alcaligenes sp

enzyme: 2-aminobenzenesulfonate 2,3-dioxygenase


organism: Hyphomicrobium sulfonivorans

substrate_name: Dimethyl sulfide

smiles: CSC

substrate: Dimethyl sulfide


enzyme_display_name: erythromycin 12 hydroxylase

organism: Saccharopolyspora erythraea

substrate_name: erythromycin D

enzyme: erythromycin 12 hydroxylase

substrate: erythromycin D


enzyme_display_name: L-ornithine N(5)-monooxygenase (NADPH)

organism: Aspergillus fumigatus

substrate_name: L-Ornithine

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

enzyme: L-ornithine N(5)-monooxygenase (NADPH)

substrate: L-Ornithine


substrate_name: NADPH

assay_conditions_summary: pH 7.6; 298.15 K; cofactor NADPH

substrate: NADPH


substrate_name: L-ornithine

substrate: L-ornithine


enzyme_display_name: 4-hydroxybenzoate 3-monooxygenase

organism: Pseudomonas aeruginosa

assay_conditions_summary: pH 6.8; 277.15 K; cofactor NADPH

enzyme: 4-hydroxybenzoate 3-monooxygenase


organism: Moraxella sp.

assay_conditions_summary: cofactor NADH


enzyme_display_name: cyclohexanone monooxygenase

organism: Acinetobacter sp.

assay_conditions_summary: pH 7.7; 298.15 K; cofactor NADPH

enzyme: cyclohexanone monooxygenase


substrate_name: cyclohexanone

substrate: cyclohexanone


organism: Ilyonectria destructans

substrate_name: Cyclopentanone

substrate: Cyclopentanone


organism: Rhodococcus sp. HI-31

proof_lines:
Raw SKiD Env: Temp=-----, pH=7.5
Raw SKiD Env: Temp=25°C, pH=9


enzyme_display_name: F-actin monooxygenase

organism: Mus musculus

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

assay_conditions_summary: pH 7; 298.15 K; cofactor NADPH

enzyme: F-actin monooxygenase


enzyme_display_name: toluene 4-monooxygenase

organism: Pseudomonas mendocina

substrate_name: Benzene

proof_lines:
Raw SKiD Env: Temp=4°C, pH=7.5
Raw SKiD Env: Temp=25°C, pH=7.5
Raw SKiD Env: Temp=25°C, pH=-----

enzyme: toluene 4-monooxygenase

substrate: Benzene


substrate_name: Toluene

substrate: Toluene


enzyme_display_name: 3-hydroxybenzoate 6-monooxygenase

organism: Pseudomonas alcaligenes

substrate_name: H+; 3-Hydroxybenzoate; NADH; O2

enzyme: 3-hydroxybenzoate 6-monooxygenase

substrate: H+; 3-Hydroxybenzoate; NADH; O2


organism: Polaromonas naphthalenivorans

substrate_name: H+; O2; 3-Hydroxybenzoate; NADH

assay_conditions_summary: cofactor NADPH

substrate: H+; O2; 3-Hydroxybenzoate; NADH


enzyme_display_name: 4-aminobenzoate 1-monooxygenase

organism: Agaricus bisporus

proof_lines:
at pH 7
at pH 8

enzyme: 4-aminobenzoate 1-monooxygenase


organism: Klebsiella oxytoca


enzyme_display_name: L-lysine N(6)-monooxygenase (NADPH)

organism: Escherichia coli

enzyme: L-lysine N(6)-monooxygenase (NADPH)


enzyme_display_name: albendazole monooxygenase

organism: Rattus norvegicus

substrate_name: albendazole

enzyme: albendazole monooxygenase

substrate: albendazole


organism: Nocardia farcinica


enzyme_display_name: flavin-containing monooxygenase

organism: Bos taurus

substrate_name: Methimazole

enzyme: flavin-containing monooxygenase

substrate: Methimazole


enzyme_display_name: 4-hydroxybenzoate 1-hydroxylase

organism: Candida parapsilosis

assay_conditions_summary: pH 7.5; 303.15 K; cofactor FAD

enzyme: 4-hydroxybenzoate 1-hydroxylase


enzyme_display_name: phenol 2-monooxygenase (NADPH)

organism: Corynebacterium glutamicum

substrate_name: phenol

assay_conditions_summary: pH 8; 318.15 K; cofactor NADPH

enzyme: phenol 2-monooxygenase (NADPH)

substrate: phenol


substrate_name: trimethylamine

substrate: trimethylamine


organism: Homo sapiens

substrate_name: sulindac sulfide

substrate: sulindac sulfide


sequence_source: UniProt:P31513 full-length canonical sequence checked, but not accepted for this kinetic tuple because Table 4 contains full-length and C-terminal-truncated FMO3 rows.


enzyme_display_name: unspecific monooxygenase

organism: Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711)

substrate_name: palmitic acid

enzyme: unspecific monooxygenase

substrate: palmitic acid


organism: Bacillus subtilis

substrate_name: NADH

assay_conditions_summary: pH 7.4; 303.15 K; cofactor NADPH

substrate: NADH


organism: Fusarium oxysporum


substrate_name: NADPH; O2; H+; Dodecanoate

substrate: NADPH; O2; H+; Dodecanoate


substrate_name: all-trans-retinoate

substrate: all-trans-retinoate


substrate_name: all-trans retinol

substrate: all-trans retinol


substrate_name: Arachidonate; O2; NADPH; H+

substrate: Arachidonate; O2; NADPH; H+


substrate_name: 17beta-estradiol (2-hydroxylation)

substrate: 17beta-estradiol (2-hydroxylation)


substrate_name: Phenacetin

substrate: Phenacetin


substrate_name: Indole

substrate: Indole


substrate_name: Melatonin

substrate: Melatonin


substrate_name: Coumarin

substrate: Coumarin


substrate_name: O2; H+; Deltamethrin; NADPH

substrate: O2; H+; Deltamethrin; NADPH


enzyme_display_name: 4-hydroxyacetophenone monooxygenase

organism: Pseudomonas fluorescens

substrate_name: NADPH (at pH 7.5)

enzyme: 4-hydroxyacetophenone monooxygenase

substrate: NADPH (at pH 7.5)


substrate_name: O2; NADPH; Fenthion; H+

substrate: O2; NADPH; Fenthion; H+


substrate_name: O2; H+; NADPH; Fenthion

substrate: O2; H+; NADPH; Fenthion


substrate_name: H+; NADPH; Fenthion; O2

substrate: H+; NADPH; Fenthion; O2


substrate_name: Cyclophosphamide

substrate: Cyclophosphamide


substrate_name: Fimasartan

substrate: Fimasartan


organism: Mus musculus

substrate_name: O2; Retinoate; H+; NADPH

substrate: O2; Retinoate; H+; NADPH


substrate_name: dodecanoic acid

smiles: CCCCCCCCCCCC(=O)O

substrate: dodecanoic acid


organism: Phanerodontia chrysosporium

substrate_name: nonanol

smiles: CCCCCCCCCO

substrate: nonanol


substrate_name: (5Z,8Z,11Z,14Z)-eicosatetraenoate (total epoxygenase activity)


substrate_name: Deltamethrin

substrate: Deltamethrin


enzyme_display_name: N-methylcoclaurine 3'-monooxygenase

organism: Eschscholzia californica

substrate_name: H+; NADPH; O2; (S)-N-Methylcoclaurine

enzyme: N-methylcoclaurine 3'-monooxygenase

substrate: H+; NADPH; O2; (S)-N-Methylcoclaurine


enzyme_display_name: vinorine hydroxylase

organism: Rauvolfia serpentina

substrate_name: vinorine

enzyme: vinorine hydroxylase

substrate: vinorine


enzyme_display_name: tabersonine 16-hydroxylase

organism: Catharanthus roseus

substrate_name: H+; NADPH; O2; Tabersonine

enzyme: tabersonine 16-hydroxylase

substrate: H+; NADPH; O2; Tabersonine


enzyme_display_name: taxane 13alpha-hydroxylase

organism: Taxus cuspidata

enzyme: taxane 13alpha-hydroxylase


enzyme_display_name: oryzalexin E synthase

organism: Oryza sativa

substrate_name: H+; NADPH; O2; ent-Sandaracopimaradiene

enzyme: oryzalexin E synthase

substrate: H+; NADPH; O2; ent-Sandaracopimaradiene


enzyme_display_name: aromatase

substrate_name: NADPH; H+; Testosterone; O2

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S312C to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aromatase(Enzyme) mutant S312C

enzyme: aromatase

substrate: NADPH; H+; Testosterone; O2


substrate_name: H+; Androst-4-ene-3,17-dione; NADPH; O2

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature D309A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aromatase(Enzyme) mutant D309A

substrate: H+; Androst-4-ene-3,17-dione; NADPH; O2


substrate_name: Testosterone; NADPH; H+; O2

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature H128Q to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aromatase(Enzyme) mutant H128Q

substrate: Testosterone; NADPH; H+; O2


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature D309N to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aromatase(Enzyme) mutant D309N


substrate_name: O2; Testosterone; H+; NADPH

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature H128A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aromatase(Enzyme) mutant H128A

substrate: O2; Testosterone; H+; NADPH