proof_lines: pH 10.0, 35°C, recombinant His-tagged enzyme


enzyme_display_name: cysteine-S-conjugate beta-lyase

organism: Mimosa pudica

substrate_name: cystathionine

source_db: brenda

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

protein_accession_database: UniProt

source_license: CC BY 4.0

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

enzyme: cysteine-S-conjugate beta-lyase

substrate: cystathionine

source: brenda


source_db: oed

source: oed


organism: Pelagibacter ubique

substrate_name: H2O; L-Cystathionine

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

substrate: H2O; L-Cystathionine


organism: Rattus norvegicus

substrate_name: S-(1,2-Dichlorovinyl)-L-cysteine; H2O

kcat_display: Not reported

km_display: Not reported

substrate: S-(1,2-Dichlorovinyl)-L-cysteine; H2O


organism: Saccharomyces cerevisiae

substrate_name: L-djenkolate

assay_conditions_summary: pH 8.5; 310.15 K; buffer 50 mM phosphate

proof_lines: in 50 mM phosphate buffer, pH 8.5, 0.02 mM pyridoxal 5-phosphate, 1 mM EDTA, at 37°C

substrate: L-djenkolate


organism: Thermotoga maritima

assay_conditions_summary: pH 8; 310.15 K; buffer recombinant His-tagged enzyme

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C, bicine buffer

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C, Tris buffer

proof_lines: recombinant His-tagged enzyme, pH 8.0, 70°C, bicine buffer


organism: Wolbachia pipientis


organism: Arabidopsis thaliana

substrate_name: L-cystine

substrate: L-cystine


organism: Bacillus sp. (in: firmicutes)

substrate_name: L-selenodjenkolate

substrate: L-selenodjenkolate


organism: Bacillus subtilis


organism: Bordetella avium

substrate_name: L-cystathionine

substrate: L-cystathionine


organism: Nicotiana tabacum

substrate_name: UDPglucose

substrate: UDPglucose


organism: Paracoccus denitrificans


organism: Pseudomonas sp. 'cruciviae'

substrate_name: pyridoxal phosphate

substrate: pyridoxal phosphate


proof_lines: mitochondria cytoplasm


organism: Salmonella enterica


organism: Brassica oleracea


organism: Cavia porcellus


organism: Escherichia coli


organism: Homo sapiens

substrate_name: beta-chloro-L-alanine

substrate: beta-chloro-L-alanine


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: L-cysteine

substrate: L-cysteine


organism: Staphylococcus haemolyticus

proof_lines: pH 8.5, 37°C, purified recombinant enzyme

proof_lines: pH 8.0, 37°C, purified recombinant enzyme


organism: Synechocystis sp. PCC 6714

substrate_name: cystine

proof_lines: estimated from steady-state measurements

substrate: cystine


organism: Vachellia farnesiana


enzyme_display_name: cellulose synthase (UDP-forming)

organism: Oryza sativa

enzyme: cellulose synthase (UDP-forming)


organism: Populus tremula x Populus tremuloides

substrate_name: UDP-alpha-D-glucose

substrate: UDP-alpha-D-glucose


organism: Gossypium hirsutum


organism: Hordeum vulgare


proof_lines: pH 7.5, 35°C, depending on individual membrane preparation


organism: Novacetimonas hansenii


organism: Eubacterium limosum

substrate_name: L-carnitine

source_db: uniprot

sequence_source: uniprot

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

substrate: L-carnitine

source: uniprot


enzyme_display_name: acetyl-CoA C-myristoyltransferase

organism: Rattus norvegicus

substrate_name: acetoacetyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: acetyl-CoA C-myristoyltransferase

substrate: acetoacetyl-CoA


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W230F/W276F/W340F


proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W188F/W230F/W276F/W300F


substrate_name: L-Cystathionine

substrate: L-Cystathionine


mutation_type: substitution


proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W230F/W276F/W300F/W340F


proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W188F/W230F/W276F/W340F


proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W188F/W276F/W300F/W340F


source_db: skid

source_license: CC BY-NC-ND 4.0

source: skid


enzyme_display_name: tricin synthase

substrate_name: S-Adenosyl-L-methionine; Tricetin

enzyme: tricin synthase

substrate: S-Adenosyl-L-methionine; Tricetin


enzyme_display_name: alpha-amino-acid esterase

substrate_name: 5'-D-Valyl-floxuridine; H2O

enzyme: alpha-amino-acid esterase

substrate: 5'-D-Valyl-floxuridine; H2O


enzyme_display_name: beta-amylase

organism: Bacillus cereus

substrate_name: H2O; Maltopentaose

enzyme: beta-amylase

substrate: H2O; Maltopentaose


proof_lines: pH 8.5, 25°C, recombinant mutants W340F and W131F/W230F/W276F/W340F


substrate_name: tricetin

substrate: tricetin


enzyme_display_name: sphingomyelin synthase

organism: Mus musculus

enzyme: sphingomyelin synthase


substrate_name: sphingomyelin

substrate: sphingomyelin


enzyme_display_name: terephthalate 1,2-dioxygenase

organism: Comamonas sp

substrate_name: TPA (at 30 degrees Celsius and at ph 7)

assay_conditions_summary: pH 7

enzyme: terephthalate 1,2-dioxygenase

substrate: TPA (at 30 degrees Celsius and at ph 7)


enzyme_display_name: sterigmatocystin 8-O-methyltransferase

organism: Aspergillus parasiticus (strain ATCC 56775 / NRRL 5862 / SRRC 143 / SU-1)

substrate_name: sterigmatocystin

enzyme: sterigmatocystin 8-O-methyltransferase

substrate: sterigmatocystin


organism: Papaver somniferum


organism: Oryza sativa subsp. japonica


enzyme_display_name: docosahexaenoic acid omega-hydroxylase

organism: Homo sapiens

substrate_name: docosanoate

proof_lines: kcat is 0.067 min(-1) with menaquinone-4 (MK-4) as substrate (PubMed:24138531). The omega-hydroxylation of VLCFAs follows dual-enzyme Michaelis-Menten kinetics, suggesting simultaneous binding of two substrate molecules. The high affinity M

enzyme: docosahexaenoic acid omega-hydroxylase

substrate: docosanoate


substrate_name: myristic acid

proof_lines: Vmax is nearly the same for myristic acid and for lauric acid and reduced about 30% for palmitic acid.

substrate: myristic acid


substrate_name: hexadecanoic acid

proof_lines: kcat is 0.088 min(-1) with menaquinone-4 (MK-4) as substrate (PubMed:24138531). kcat is 1.8 min(-1) with hexadecanoic acid as substrate. kcat is 1.0 min(-1) with (9Z)-octadecenoic acid as substrate. kcat is 0.54 min(-1) with (5Z,8Z,11Z,14Z)

substrate: hexadecanoic acid


substrate_name: leukotriene B4

proof_lines: The omega-hydroxylation of VLCFAs follows dual-enzyme Michaelis-Menten kinetics, suggesting simultaneous binding of two substrate molecules. The high affinity Michaelis-Menten constants are shown.

substrate: leukotriene B4


enzyme_display_name: pantetheine hydrolase

organism: Equus caballus

substrate_name: pantetheine

enzyme: pantetheine hydrolase

substrate: pantetheine


proof_lines: apparent Km value, soluble recombinant vanin-1, pH 7.7, 25°C

proof_lines: apparent Km value, HPLC assay, pH 7.7, 25°C


organism: Sus scrofa

substrate_name: (R)-pantetheine

substrate: (R)-pantetheine


enzyme_display_name: tyrosine isonitrile desaturase

organism: Erwinia amylovora (strain CFBP1430)

substrate_name: tyrosine isonitrile

enzyme: tyrosine isonitrile desaturase

substrate: tyrosine isonitrile


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)


substrate_name: Unknown

ki_unit: mM

substrate: Unknown


organism: Erwinia amylovora


proof_lines: isoform SMS2, in 50 mM Tris–HCl (pH 7.4), at 22°C


proof_lines: isoform SMS1, in 50 mM Tris–HCl (pH 7.4), at 22°C


substrate_name: 3-Chloro-L-alanine; H2O

substrate: 3-Chloro-L-alanine; H2O


organism: Lacticaseibacillus casei

substrate_name: L-cysteine

assay_conditions_summary: pH 5.5; 310.15 K; buffer sodium phosphate

proof_lines: Ctl1, in sodium phosphate buffer, at pH 6.8 and 37°C

proof_lines: Ctl1, in sodium phosphate buffer, at pH 5.5 and 37°C

substrate: L-cysteine


enzyme_display_name: D-proline dehydrogenase

organism: Unknown

substrate_name: D-proline

enzyme: D-proline dehydrogenase

substrate: D-proline


organism: Pyrobaculum islandicum

proof_lines: Ketjen Black/D-Pro dehydrogenase electrode

proof_lines: carbon nanotube gel/D-Pro dehydrogenase electrode


enzyme_display_name: bacillolysin

organism: Bacillus subtilis

substrate_name: Z-Phe-Leu-Ala-OH; H2O

enzyme: bacillolysin

substrate: Z-Phe-Leu-Ala-OH; H2O


organism: Hordeum vulgare


enzyme_display_name: GDP-mannose 3,5-epimerase

organism: Arabidopsis thaliana

substrate_name: GDP-D-mannose

proof_lines: recombinant enzyme with N-terminal glutathione-S-transferase tag

proof_lines: recombinant enzyme with N-terminal His-tag

enzyme: GDP-mannose 3,5-epimerase

substrate: GDP-D-mannose


organism: Auxenochlorella pyrenoidosa

proof_lines: pH and temperature not specified in the publication


organism: Oryza sativa

substrate_name: GDP-alpha-D-mannose

substrate: GDP-alpha-D-mannose


assay_conditions_summary: cofactor NAD+

proof_lines:
in presence of 0.02 mM NAD+
without NAD+


enzyme_display_name: prenylcysteine oxidase

substrate_name: free farnesylcysteine

proof_lines: kcat is 8000 sec(-1) for free farnesylcysteine.

enzyme: prenylcysteine oxidase

substrate: free farnesylcysteine


organism: Bos taurus


substrate_name: geranylgeranylcysteine

substrate: geranylgeranylcysteine


substrate_name: farnesyl-L-cysteine

substrate: farnesyl-L-cysteine


substrate_name: beta-carotene

proof_lines: pH 7.0, temperature not specified in the publication

proof_lines: recombinant enzyme, pH not specified in the publication, 28°C

substrate: beta-carotene


km_unit: mM

proof_lines: addition of methanol to in vitro assays has a strong stimulatory effect.

data_origin: experimental


organism: Lathyrus oleraceus


enzyme_display_name: corticosterone 18-monooxygenase

substrate_name: corticosterone

proof_lines: pH 7.4, 37°C. The production of 18-OH corticosterone from corticosterone cannot be isolated from the production of aldosterone; therefore, the value is not true a steady-state parameter because the data reports on both reactions simultaneou

enzyme: corticosterone 18-monooxygenase

substrate: corticosterone


enzyme_display_name: cystine reductase

substrate_name: L-cystine

enzyme: cystine reductase

substrate: L-cystine


organism: Histoplasma capsulatum

assay_conditions_summary: cofactor NADH


organism: Saccharomyces cerevisiae


organism: Homo sapiens

substrate_name: lactosylceramide

substrate: lactosylceramide


organism: Oryctolagus cuniculus


organism: Rattus norvegicus

substrate_name: UDP-galactose

substrate: UDP-galactose


substrate_name: CMP-N-acetylneuraminate

substrate: CMP-N-acetylneuraminate


substrate_name: sialyl lactoetraosylceramide

proof_lines:
isoform ST6GalNAc V, pH 6.0, 37°C
isoform ST6GalNAc VI, pH 6.0, 37°C

substrate: sialyl lactoetraosylceramide


organism: Lancefieldella rimae

proof_lines: pH 7.8, temperature not specified in the publication


organism: Treponema denticola


organism: Mesorhizobium loti


organism: Pseudomonas sp.


enzyme_display_name: homogentisate solanesyltransferase

substrate_name: farnesyl diphosphate

proof_lines: with respect to farnesyl diphosphate, at pH 8.0 and 32°C

proof_lines: with respect to homogentisate, at pH 8.0 and 32°C

enzyme: homogentisate solanesyltransferase

substrate: farnesyl diphosphate


organism: Chlamydomonas reinhardtii

substrate_name: homogentisate

substrate: homogentisate


enzyme_display_name: galacturonokinase

substrate_name: D-galacturonate

assay_conditions_summary: cofactor ATP

enzyme: galacturonokinase

substrate: D-galacturonate


substrate_name: ATP

substrate: ATP


enzyme_display_name: 4-sulfomuconolactone hydrolase

organism: Agrobacterium tumefaciens

sequence_source: UniProtKB:A6XIG7 direct accession anchor; sequence is present in the live entry but was not imported into this row.

assay_conditions_summary: pH 8

proof_lines: pH 8.0, temperature not specified in the publication

enzyme: 4-sulfomuconolactone hydrolase


organism: Hydrogenophaga intermedia

sequence_source: UniProtKB:A6XIG6 direct accession anchor; sequence is present in the live entry but was not imported into this row.


organism: Lancefieldella rimae (strain ATCC 49626 / DSM 7090 / CCUG 31168 / NBRC 15546 / VPI D140H-11A)

sequence_source: Trusted canonical sequence is preserved in the selected source record for UniProt B9CM12; not emitted in this review.

proof_lines: kcat is 3.7 sec(-1) with 2-aminoethylphosphonate as substrate. kcat is 0.7 sec(-1) with phosphocholine as substrate.


organism: Treponema denticola (strain ATCC 35405 / DSM 14222 / CIP 103919 / JCM 8153 / KCTC 15104)

substrate_name: 2-aminoethylphosphonate (for cytidylyltransferase activity)

sequence_source: Trusted canonical sequence is preserved in the selected source record for UniProt Q73MU2; not emitted in this review.

proof_lines: kcat is 1.05 sec(-1) with 2-aminoethylphosphonate as substrate for cytidylyltransferase activity.


organism: Agrobacterium radiobacter

substrate_name: 4-sulfomuconolactone (at pH 8)

sequence_source: Trusted canonical sequence is preserved in the selected source record for UniProt A6XIG7; not emitted in this review.

proof_lines: kcat is 400 min(-1) with 4-sulfomuconolactone as substrate (at pH 8).

substrate: 4-sulfomuconolactone (at pH 8)


sequence_source: Trusted canonical sequence is preserved in the selected source record for UniProt A6XIG6; not emitted in this review.

proof_lines: kcat is 2700 min(-1) with 4-sulfomuconolactone (at pH 8).


enzyme_display_name: sulfite dehydrogenase (quinone)

organism: Aquifex aeolicus

substrate_name: sulfite

proof_lines: pH 8.3, 55°C, with Nitro-blue tetrazolium

enzyme: sulfite dehydrogenase (quinone)

substrate: sulfite


enzyme_display_name: carboxypeptidase Taq

organism: Trypanosoma cruzi

proof_lines:
in the presence of Co2+
in the absence of Co2+

enzyme: carboxypeptidase Taq


organism: Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

verification_status: corrected

proof_lines: kcat is 307.3 min(-1) for 2-(acetamidomethylene)succinate.

status: corrected


enzyme_display_name: streptothricin hydrolase

organism: Streptomyces noursei

substrate_name: streptothricin-F

enzyme: streptothricin hydrolase

substrate: streptothricin-F


enzyme_display_name: NAD(+) 3'-kinase

organism: Xanthomonas oryzae pv. oryzicola

substrate_name: NaAD+

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NAD+

proof_lines: with NAD+ as cosubstrate, at pH 7.5 and 25°C

proof_lines: with NAAD+ as cosubstrate, at pH 7.5 and 25°C

enzyme: NAD(+) 3'-kinase

substrate: NaAD+


organism: Bos taurus


organism: Homo sapiens


substrate_name: ganglioside GM1b

substrate: ganglioside GM1b


substrate_name: homogentisate (in the presence of 200 uM farnesyl diphosphate)


organism: Arabidopsis thaliana


enzyme_display_name: protein S-acyltransferase

organism: Rattus norvegicus

substrate_name: L-glutamate (with 2 mM of Mn(2+))

enzyme: protein S-acyltransferase

substrate: L-glutamate (with 2 mM of Mn(2+))


organism: Mus musculus

substrate_name: palmitoyl-CoA

assay_conditions_summary: pH 7.4; 298.15 K; cofactor CoA

substrate: palmitoyl-CoA


organism: Neurospora crassa

assay_conditions_summary: pH 7.2; buffer 50 mM phosphate


enzyme_display_name: salicylate 5-hydroxylase

organism: Ralstonia sp.

substrate_name: salicylate

assay_conditions_summary: pH 6.4; 295.15 K; buffer 50 mM MES; cofactor NADPH

enzyme: salicylate 5-hydroxylase

substrate: salicylate


proof_lines: 4°C, pH not specified in the publication

proof_lines: 23°C, pH not specified in the publication


enzyme_display_name: 3-hydroxyphenylacetate 6-hydroxylase

organism: Flavobacterium sp.

assay_conditions_summary: cofactor NADPH

enzyme: 3-hydroxyphenylacetate 6-hydroxylase


organism: Hedera helix

substrate_name: stearoyl-[acyl-carrier protein]

proof_lines: pH and temperature not specified in the publication

substrate: stearoyl-[acyl-carrier protein]


enzyme_display_name: ecdysone 20-monooxygenase

organism: Locusta migratoria migratorioides

substrate_name: ecdysone

enzyme: ecdysone 20-monooxygenase

substrate: ecdysone


organism: Manduca sexta

proof_lines:
mitochondrial enzyme system
microsomal enzyme
mitochondrial enzyme


organism: Schistocerca gregaria

substrate_name: alpha-ecdysone

substrate: alpha-ecdysone


organism: Spinacia oleracea


substrate_name: UDP-glucuronic acid

substrate: UDP-glucuronic acid


substrate_name: chondroitin sulfate undecasaccharide

substrate: chondroitin sulfate undecasaccharide


substrate_name: C11-oligosaccharide of chondroitin sulfate A


enzyme_display_name: isovaleryl-homoserine lactone synthase

organism: Bradyrhizobium diazoefficiens

substrate_name: isovaleryl-ACP

assay_conditions_summary: pH 7.3; cofactor CoA

proof_lines: pH 7.3, temperature not specified in the publication

enzyme: isovaleryl-homoserine lactone synthase

substrate: isovaleryl-ACP


organism: Acinetobacter calcoaceticus

proof_lines: with 1,2-dipalmitoyl-rac-glycerol as cosubstrate, pH and temperature not specified in the publication

proof_lines: with 1-hexadecanol as cosubstrate, pH and temperature not specified in the publication


organism: Marinobacter nauticus

substrate_name: n-Hexanol

sequence_source: UniProtKB:A1TX06 direct accession lookup (canonical entry only)

proof_lines: mutant enzyme M405F, pH and temperature not specified in the publication

substrate: n-Hexanol


proof_lines: mutant enzyme L356F, pH and temperature not specified in the publication


proof_lines: wild type enzyme, pH and temperature not specified in the publication


enzyme_display_name: 3-oxoglutarate synthase

organism: Atropa belladonna

substrate_name: malonyl-CoA

enzyme: 3-oxoglutarate synthase

substrate: malonyl-CoA


enzyme_display_name: galactan endo-beta-1,3-galactanase

organism: Flammulina velutipes

sequence_source: UniProtKB:F7J1C8 (reviewed entry; sequence present)

enzyme: galactan endo-beta-1,3-galactanase


sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

proof_lines: kcat is 7.56 sec(-1) with beta-1,3-galactan. kcat is 5.47 sec(-1) with beta-1,3-galactotriose. kcat is 6.50 sec(-1) with beta-1,3-galactotetraose.


enzyme_display_name: limonoid glucosyltransferase

organism: Citrus maxima

substrate_name: UDP-glucose

proof_lines: pH 7.8, 37°C, enzyme immobilized covalently on cellulose carbonate

proof_lines: pH 7.8, 37°C, enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde

enzyme: limonoid glucosyltransferase

substrate: UDP-glucose


organism: Citrus maxima x Citrus paradisi

substrate_name: limonin

substrate: limonin


enzyme_display_name: beta-diketone hydrolase

organism: Sphingopyxis sp. (strain 113P3)

substrate_name: polyvinyl alcohol

enzyme: beta-diketone hydrolase

substrate: polyvinyl alcohol


organism: Sphingomonas sp.

substrate_name: p-nitrophenyl acetate

substrate: p-nitrophenyl acetate


enzyme_display_name: delphinidin 3',5'-O-glucosyltransferase

organism: Clitoria ternatea

proof_lines: delphinidin 3-O-(6-O-malonyl)-beta-D-glucoside-3-O-beta-glucoside as substrate

proof_lines: delphinidin 3-O-(6-O-malonyl)-beta-D-glucoside as substrate

enzyme: delphinidin 3',5'-O-glucosyltransferase


organism: Nostoc sp. PCC 7120 = FACHB-418


organism: Canis lupus familiaris


organism: Cavia porcellus


organism: Gallus gallus


organism: Mesocricetus auratus

substrate_name: UDP-N-acetyl-D-galactosamine

substrate: UDP-N-acetyl-D-galactosamine


organism: Lactococcus lactis


organism: Escherichia coli


substrate_name: MKATKLVLGAVILGSTLLAGCSSN

substrate: MKATKLVLGAVILGSTLLAGCSSN


enzyme_display_name: photosystem I

organism: Spinacia oleracea

substrate_name: Unknown

enzyme: photosystem I

substrate: Unknown


organism: Synechocystis sp.

substrate_name: reduced plastocyanin

substrate: reduced plastocyanin


enzyme_display_name: opheline kinase

organism: Ophelia neglecta

substrate_name: phosphotaurocyamine

assay_conditions_summary: 303.15 K; cofactor ATP

enzyme: opheline kinase

substrate: phosphotaurocyamine


enzyme_display_name: beta-glucogallin O-galloyltransferase

organism: Quercus robur

enzyme: beta-glucogallin O-galloyltransferase


organism: Arabidopsis thaliana


organism: Flaveria bidentis

substrate_name: quercetin 3,3'-disulfate

proof_lines: cosubstrate quercetin 3,3-disulfate, isoenzyme II

substrate: quercetin 3,3'-disulfate


organism: Dictyostelium discoideum

substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


organism: Mus musculus

substrate_name: [D-glucuronic acid-N-acetyl-glucosamine]4 -D-glucuronic acid-2,5-anhydro-D-mannose


enzyme_display_name: N-sulfoglucosamine sulfohydrolase

organism: Homo sapiens

substrate_name: heparan sulfate

proof_lines: substrates deriving from heparan sulfate, unamplified recombinant enzyme

proof_lines: substrates deriving from heparan sulfate, gene-amplified recombinant enzyme

enzyme: N-sulfoglucosamine sulfohydrolase

substrate: heparan sulfate


organism: Pedobacter heparinus

substrate_name: Glucosamine 2,6-disulfate

substrate: Glucosamine 2,6-disulfate


organism: Rattus norvegicus

substrate_name: heparin

substrate: heparin


enzyme_display_name: L-arabinokinase

substrate_name: L-arabinose

enzyme: L-arabinokinase

substrate: L-arabinose


substrate_name: ATP

assay_conditions_summary: pH 8; 303.15 K; cofactor ATP

substrate: ATP


assay_conditions_summary: pH 6.5; 308.15 K; cofactor ATP


enzyme_display_name: scutelarin

organism: Bothrops cotiara

substrate_name: prothrombin

proof_lines:
22°C, pH 7.4, presence of S2238
22°C, pH 7.4

enzyme: scutelarin

substrate: prothrombin


organism: Oxyuranus scutellatus


enzyme_display_name: thiocyanate hydrolase

organism: Thiobacillus thioparus

substrate_name: thiocyanate

enzyme: thiocyanate hydrolase

substrate: thiocyanate


organism: Thiohalophilus thiocyanatoxydans


substrate_name: Methacrylonitrile

substrate: Methacrylonitrile


enzyme_display_name: citryl-CoA lyase

organism: Hydrogenobacter thermophilus

substrate_name: citryl-CoA

assay_conditions_summary: pH 8; 343.15 K; cofactor CoA

enzyme: citryl-CoA lyase

substrate: citryl-CoA


organism: Escherichia coli

substrate_name: acetyl-CoA

assay_conditions_summary: cofactor CoA

substrate: acetyl-CoA


substrate_name: oxaloacetate

substrate: oxaloacetate


enzyme_display_name: glutamyl aminopeptidase

organism: Bitis gabonica rhinoceros

substrate_name: L-Glutamic acid gamma-(7-amido-4-methylcoumarin); H2O

enzyme: glutamyl aminopeptidase


organism: Sus scrofa

substrate_name: Glu-p-nitroanilide; H2O

substrate: Glu-p-nitroanilide; H2O


enzyme_display_name: jasmonoyl--L-amino acid ligase

substrate_name: L-Isoleucine; Jasmonic acid; ATP

enzyme: jasmonoyl--L-amino acid ligase

substrate: L-Isoleucine; Jasmonic acid; ATP


substrate_name: Jasmonic acid; Glutamine; ATP

substrate: Jasmonic acid; Glutamine; ATP


enzyme_display_name: adenosylhomocysteinase

organism: Synechocystis sp. PCC 6803

substrate_name: S-adenosyl-L-homocysteine

sequence_source: UniProtKB direct accession lookup

enzyme: adenosylhomocysteinase

substrate: S-adenosyl-L-homocysteine


organism: Thermotoga maritima

substrate_name: adenosine

source_license: CC BY 4.0; CC BY-NC-ND 4.0

proof_lines:
at pH 6.5 and 75°C
Raw SKiD Env: Temp=75°C, pH=6.5

source_databases_merged: brenda skid

substrate: adenosine


organism: Trypanosoma cruzi


organism: Branchiostoma belcheri tsingtauense

substrate_name: L-homocysteine

substrate: L-homocysteine


organism: Corynebacterium efficiens

substrate_name: DL-homocysteine

substrate: DL-homocysteine


organism: Cryptosporidium parvum

substrate_name: S-adenosyl-DL-homocysteine

substrate: S-adenosyl-DL-homocysteine


organism: Cytophaga hutchinsonii

proof_lines:
pH 7.5, 22°C
at pH 7.5 and 22°C


organism: Homo sapiens


proof_lines: recombinant wild type enzyme, at pH 7.2 and 37°C


proof_lines:
pH 6.5, 41°C
pH 7.5, 37°C
Raw SKiD Env: Temp=37°C, pH=7.2


organism: Plasmodium falciparum

substrate_name: Unknown

substrate: Unknown


organism: Pseudomonas aeruginosa


proof_lines: pH 7.5, 20°C, recombinant wild-type enzyme, in presence of Rb+

proof_lines: pH 7.5, 20°C, recombinant wild-type enzyme, in presence of Li+


proof_lines: pH 7.5, 20°C, recombinant mutant Q65A, in presence of K+


proof_lines: pH and temperature not specified in the publication


organism: Pyrococcus horikoshii

assay_conditions_summary: pH 6.5; 333.15 K; cofactor NAD+

proof_lines: in the presence of 0.5 mM NAD+, at pH 6.5 and 60°C

proof_lines: in the absence of NAD+, at pH 6.5 and 60°C


organism: Rattus norvegicus

assay_conditions_summary: pH 7.2; 295.15 K; buffer 50 mM potassium phosphate

proof_lines: at 22°C in 50 mM potassium phosphate buffer, at pH 7.2


proof_lines: pH 7.5, 20°C, wild-type enzyme, 100 mM Na+

proof_lines: pH 7.5, 20°C, wild-type enzyme, without monovalent metal ion

proof_lines: pH 7.5, 20°C, wild-type enzyme, 100 mM Rb+


proof_lines: pH 7.5, 20°C, mutant enzyme R24E, 100 mM K+


proof_lines:
liver enzyme
column-retained isozyme
flow-through isozyme


proof_lines:
30°C, pH 7.6, wild-type enzyme
wild-type


organism: Pyrococcus furiosus


organism: Mus musculus

proof_lines:
mutant Sahn 12
mutant Sahn 12 enzyme


organism: Saccharolobus solfataricus


mutation_signature: truncated recombinant enzyme truncated recombinant enzyme

mutation_type: truncation


organism: Lupinus luteus

substrate_name: cysteine

substrate: cysteine


organism: Mesocricetus auratus


organism: Acinetobacter calcoaceticus


organism: Alcaligenes faecalis


organism: Beta vulgaris


organism: Bos taurus

proof_lines:
no phosphate added
phosphate added


organism: Bradyrhizobium elkanii

proof_lines:
23°C, pH 7.0
at pH 8.0 and 25°C


organism: Corynebacterium glutamicum


organism: Cryphonectria parasitica


organism: Dictyostelium discoideum


organism: Mycobacterium tuberculosis


organism: Plasmodium falciparum


organism: Trichomonas vaginalis

proof_lines:
native enzyme
recombinant enzyme


organism: Trypanosoma cruzi


enzyme_display_name: alpha-L-rhamnosidase

organism: Aspergillus aculeatus

enzyme: alpha-L-rhamnosidase


substrate_name: p-nitrophenyl-alpha-L-rhamnopyranoside

substrate: p-nitrophenyl-alpha-L-rhamnopyranoside


organism: Aspergillus niger

substrate_name: 4-nitrophenyl alpha-L-rhamnopyranoside

substrate: 4-nitrophenyl alpha-L-rhamnopyranoside


organism: Aspergillus terreus


source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

proof_lines: pH 6.5, 65°C, in presence of 0.6 M sorbitol


substrate_name: p-Nitrophenyl-alpha-L-rhamnopyranoside

substrate: p-Nitrophenyl-alpha-L-rhamnopyranoside


organism: Aspergillus tubingensis

substrate_name: naringin

substrate: naringin


organism: Bacillus sp. (in: firmicutes)

assay_conditions_summary: pH 7


proof_lines: crystallized complex with interacting site analyzed


organism: Bacteroides thetaiotaomicron

proof_lines:
50°C, pH 6.5
Raw SKiD Env: Temp=50°C, pH=6.5


organism: Caldicellulosiruptor bescii

proof_lines: recombinant wild-type enzyme, pH 7.0, 80°C


organism: Dictyoglomus thermophilum


proof_lines:
pH 5.0, 37°C
Raw SKiD Env: Temp=37°C, pH=5


organism: Lactiplantibacillus plantarum


substrate_name: hesperidin

substrate: hesperidin


proof_lines: pH 7.0, 30°C, recombinant isozyme Ram1, calculated by BRENDA


organism: Novosphingobium sp. PP1Y


substrate_name: quercitrin

substrate: quercitrin


proof_lines: pH 6.9, temperature not specified in the publication


substrate_name: rutin

substrate: rutin


organism: Thermoclostridium stercorarium subsp. stercorarium


organism: Thermoclostridium stercorarium subsp. thermolacticum


organism: Thermomicrobia bacterium

proof_lines: pH 7.0, 50°C, recombinant RhmB, versus 4-nitrophenyl alpha-L-rhamnopyranoside


proof_lines: pH 7.5, 50°C, recombinant RhmA, versus 4-nitrophenyl alpha-L-rhamnopyranoside


organism: Thermotoga maritima


organism: Thermotoga neapolitana


organism: Alternaria sp. L1


organism: Acrostalagmus luteoalbus


organism: Alternaria alternata


organism: Aspergillus niger


organism: Aspergillus oryzae


substrate_name: neohesperidin

proof_lines: recombinant wild-type enzyme, pH 5.0, 50°C, calculated by BRENDA

substrate: neohesperidin


organism: Aspergillus terreus


organism: Bacteroides JY-6


organism: Fagopyrum esculentum


organism: Fusarium verticillioides

proof_lines: pH 10.5 (0.2M sodium phosphate buffer), 50°C


organism: Fusobacterium sp.


proof_lines: recombinant enzyme, pH 7.5, temperature not specified in the publication


organism: Ogataea angusta


organism: Sphingomonas paucimobilis


organism: Turbo cornutus

substrate_name: p-nitrophenyl alpha-L-rhamnoside

substrate: p-nitrophenyl alpha-L-rhamnoside


organism: Papiliotrema laurentii


organism: Penicillium decumbens

proof_lines: pH 6.7, 30°C, versus 4-nitrophenyl alpha-L-rhamnopyranoside


organism: Penicillium sp.

proof_lines:
soluble, narginase activity
immobilized, narginase activity


organism: Penicillium tardum

proof_lines: pH 5.0, 37Â°C, versus 4-nitrophenyl alpha-L-rhamnopyranoside


enzyme_display_name: methanethiol oxidase

organism: Thiobacillus thioparus

substrate_name: methanethiol

enzyme: methanethiol oxidase

substrate: methanethiol


enzyme_display_name: sucrose-phosphate synthase

organism: Anabaena sp.

substrate_name: UDP-glucose

proof_lines: SPS-II SPS-I

enzyme: sucrose-phosphate synthase

substrate: UDP-glucose


organism: Brassica napus


organism: Hordeum vulgare


organism: Ipomoea batatas

substrate_name: D-fructose 6-phosphate

substrate: D-fructose 6-phosphate


organism: Nicotiana tabacum

substrate_name: glutathione

substrate: glutathione


organism: Silene vulgaris

substrate_name: bimane-glutathione

substrate: bimane-glutathione


organism: Solanum lycopersicum


organism: Spinacia oleracea

proof_lines: in presence of 2.5 mM glucose 6-phosphate

proof_lines: in presence and absence of glucose 6-phosphate


organism: Synechocystis sp.

assay_conditions_summary: pH 8; cofactor ADP

proof_lines: pH 8.0, cosubstrate: UDP-glucose, enzyme expressed in Nicotiana tabacum

proof_lines: pH 8.0, cosubstrate: UDP-glucose, enzyme expressed in Escherichia coli

proof_lines: pH 8.0, enzyme expressed in Nicotiana tabacum


organism: Trifolium repens


organism: Triticum aestivum


organism: Lathyrus oleraceus

proof_lines: in presence of 0.5 mM glucose 6-phosphate


organism: Neltuma juliflora

proof_lines: Km is not effected by addition of 20 mM glucose 6-phosphate or 15 mM phosphate

proof_lines: in presence of 20 mM glucose 6-phosphate


organism: Oryza sativa


organism: Solanum tuberosum


organism: Silene cucubalus

substrate_name: Glutathione; [Glu(-Cys)]n-Gly

substrate: Glutathione; [Glu(-Cys)]n-Gly


organism: Saccharum officinarum

substrate_name: D-Fructose 6-phosphate; UDP-D-glucose

substrate: D-Fructose 6-phosphate; UDP-D-glucose


enzyme_display_name: rubredoxin--NAD(+) reductase

organism: Megalodesulfovibrio gigas

substrate_name: Desulfovibrio gigas rubredoxin

assay_conditions_summary: cofactor NADH

enzyme: rubredoxin--NAD(+) reductase

substrate: Desulfovibrio gigas rubredoxin


enzyme_display_name: phosphosulfolactate synthase

organism: Methanocaldococcus jannaschii

substrate_name: phosphoenolpyruvate

enzyme: phosphosulfolactate synthase

substrate: phosphoenolpyruvate


substrate_name: Phosphoenolpyruvate; Sulfite

substrate: Phosphoenolpyruvate; Sulfite


enzyme_display_name: isopiperitenol dehydrogenase

organism: Mentha x piperita

substrate_name: (+)-trans-isoperitenol

enzyme: isopiperitenol dehydrogenase

substrate: (+)-trans-isoperitenol


enzyme_display_name: lignostilbene alphabeta-dioxygenase

organism: Sphingomonas paucimobilis

proof_lines:
isozyme IV
isozyme I
pH 8.5, 30°C

enzyme: lignostilbene alphabeta-dioxygenase


organism: Clostridium acetobutylicum

substrate_name: NADH; H+; Oxidized rubredoxin

substrate: NADH; H+; Oxidized rubredoxin


organism: Homo sapiens

substrate_name: H2O; O2; Methanethiol

proof_lines: pH and temperature not specified in the publication

substrate: H2O; O2; Methanethiol


organism: Hyphomicrobium sp.


organism: Arabidopsis thaliana


organism: Euglena gracilis

assay_conditions_summary: pH 8

proof_lines: in the presence of 0.02 mM GSH, at pH 8.0, temperature not specified in the publication

proof_lines: in the presence of 0.04 mM GSH, at pH 8.0, temperature not specified in the publication


organism: Methylobacillus flagellatus

proof_lines: pH 6.5, 45°C, recombinant His6-tagged enzyme, in presence of fructose 1,6-bisphosphate


organism: Pisum sativum

substrate_name: UDP; Sucrose

substrate: UDP; Sucrose


enzyme_display_name: cysteate synthase

organism: Methanosarcina acetivorans

substrate_name: Sulfite; O-Phospho-L-serine

enzyme: cysteate synthase

substrate: Sulfite; O-Phospho-L-serine


enzyme_display_name: lupanine 17-hydroxylase (cytochrome c)

organism: Pseudomonas sp.

substrate_name: sparteine

enzyme: lupanine 17-hydroxylase (cytochrome c)

substrate: sparteine


enzyme_display_name: trans-anol O-methyltransferase

organism: Pimpinella anisum

substrate_name: isoeugenol

proof_lines: pH 7.5, temperature not specified in the publication

enzyme: trans-anol O-methyltransferase

substrate: isoeugenol


organism: Anethum foeniculum

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


enzyme_display_name: inositol 1-methyltransferase

organism: Lathyrus oleraceus

enzyme: inositol 1-methyltransferase


substrate_name: S-Adenosyl-L-methionine; myo-Inositol

substrate: S-Adenosyl-L-methionine; myo-Inositol


organism: Unknown

substrate_name: GTP

substrate: GTP


enzyme_display_name: phloroisovalerophenone synthase

organism: Humulus lupulus

enzyme: phloroisovalerophenone synthase


substrate_name: isovaleryl-CoA

substrate: isovaleryl-CoA


organism: Artemia salina


organism: Artemia sp.


enzyme_display_name: deoxyribonuclease (pyrimidine dimer)

organism: Tequatrovirus T4

substrate_name: DNA 14mer containing a TT-dimer

enzyme: deoxyribonuclease (pyrimidine dimer)

substrate: DNA 14mer containing a TT-dimer


proof_lines: pH 7.0, temperature not specified in the publication


organism: Mus musculus


organism: Bos taurus

substrate_name: UDP-galactose

substrate: UDP-galactose


organism: Homo sapiens

substrate_name: GlcNAc-B-S-pNP

substrate: GlcNAc-B-S-pNP


substrate_name: UDP-Gal

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

substrate: UDP-Gal


enzyme_display_name: nerylneryl diphosphate synthase

organism: Solanum lycopersicum

substrate_name: isopentenyl diphosphate

enzyme: nerylneryl diphosphate synthase

substrate: isopentenyl diphosphate


organism: Rhodocyclus tenuis

substrate_name: Sulfide

proof_lines: with sulfide as cosubstrate, pH and temperature not specified in the publication

proof_lines: with thiosulfate as cosubstrate, pH and temperature not specified in the publication

substrate: Sulfide


organism: Arabidopsis thaliana

substrate_name: O-acetyl-L-serine

substrate: O-acetyl-L-serine


organism: Glycine max

substrate_name: (-)-glycinol

substrate: (-)-glycinol


enzyme_display_name: naringenin 8-dimethylallyltransferase

organism: Sophora flavescens

substrate_name: dimethylallyl diphosphate

enzyme: naringenin 8-dimethylallyltransferase

substrate: dimethylallyl diphosphate


organism: Salmonella enterica subsp. enterica serovar Typhimurium

proof_lines: at pH 7.6, temperature not specified in the publication


proof_lines: using microsomes of cultured soybean cells


substrate_name: naringenin

substrate: naringenin


substrate_name: 3,3-dimethylallyl diphosphate

substrate: 3,3-dimethylallyl diphosphate


enzyme_display_name: dihydroxyphenylalanine transaminase

organism: Cavia porcellus

enzyme: dihydroxyphenylalanine transaminase


substrate_name: 2-Oxoglutarate; L-Dopa

substrate: 2-Oxoglutarate; L-Dopa


organism: Rattus norvegicus


enzyme_display_name: succinyl-CoA--L-malate CoA-transferase

organism: Chloroflexus aurantiacus

substrate_name: (S)-malate

assay_conditions_summary: 328.15 K; cofactor CoA

proof_lines: 55°C, pH not specified in the publication, recombinant enzyme

enzyme: succinyl-CoA--L-malate CoA-transferase

substrate: (S)-malate


substrate_name: succinyl-CoA

substrate: succinyl-CoA


substrate_name: phosphatidylinositol 3,5-biphosphate

substrate: phosphatidylinositol 3,5-biphosphate


organism: Bifidobacterium longum subsp. Longum

proof_lines:
pH 5.5 and 45Â°C
at pH 5.5 and 45Â°C


organism: Mycolicibacterium hassiacum

proof_lines:
at pH 6.5 and 45°C
at pH 6.5 and 37°C


enzyme_display_name: UDP-sulfoquinovose synthase

organism: Arabidopsis sp.

substrate_name: UDP-glucose

enzyme: UDP-sulfoquinovose synthase

substrate: UDP-glucose


enzyme_display_name: aminodeoxychorismate lyase

substrate_name: D-aspartate

enzyme: aminodeoxychorismate lyase

substrate: D-aspartate


organism: Mycobacterium tuberculosis

sequence_source: UniProt Q79FW0 supports canonical identity only; no row-specific variant sequence was established.


sequence_source: UniProt Q79FW0 supports canonical identity only; selected row sequence remains unresolved.


enzyme_display_name: phosphogluconate dehydrogenase [NAD(P)(+)-dependent, decarboxylating]

organism: Pseudomonas sp.

substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

proof_lines: using NADP+ as cosubstrate, pH and temperature not specified in the publication

substrate: NADP+


organism: Thermotoga maritima

assay_conditions_summary: pH 7.5; 353.15 K; buffer 50 mM HEPES; cofactor NADP+

proof_lines: with NADP+ as cosubstrate, in 50 mM HEPES buffer (pH 7.5), at 80°C

enzyme: phosphogluconate dehydrogenase [NAD(P)(+)-dependent, decarboxylating]


organism: Ovis aries


organism: Sus scrofa

substrate_name: N-acetyl-D-galactosaminyl-alpha-benzyl

substrate: N-acetyl-D-galactosaminyl-alpha-benzyl


enzyme_display_name: apigenin 4'-O-methyltransferase

organism: Robinia pseudoacacia

substrate_name: apigenin

enzyme: apigenin 4'-O-methyltransferase

substrate: apigenin


enzyme_display_name: glucosamine N-acetyltransferase

organism: Avena sativa

substrate_name: D-glucosamine

proof_lines:
CHAPS-solubilized enzyme
membrane-bound enzyme

enzyme: glucosamine N-acetyltransferase

substrate: D-glucosamine


organism: Clostridium acetobutylicum

substrate_name: acetyl-CoA

assay_conditions_summary: pH 7.3; 298.15 K; cofactor CoA

substrate: acetyl-CoA


substrate_name: ganglioside GD3

substrate: ganglioside GD3


organism: Equus caballus


organism: Mus musculus

substrate_name: UDP-N-acetyl-D-glucosamine

proof_lines: mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C

substrate: UDP-N-acetyl-D-glucosamine


organism: Homo sapiens

substrate_name: UDP-N-acetyl-D-galactosamine

substrate: UDP-N-acetyl-D-galactosamine


substrate_name: UDP-N-acetylgalactosamine

substrate: UDP-N-acetylgalactosamine


organism: Dictyostelium discoideum

proof_lines:
cosubstrate Man9GlcNAc
i.e. Man9GlcNAc, intersected product
intersected product


enzyme_display_name: Transferred entry: 2.4.3.1

substrate_name: Lactosamine; CMP-N-acetylneuraminate

enzyme: Transferred entry: 2.4.3.1

substrate: Lactosamine; CMP-N-acetylneuraminate


organism: Rattus norvegicus

substrate_name: Fetuin; CMP-N-acetylneuraminate

substrate: Fetuin; CMP-N-acetylneuraminate


enzyme_display_name: Transferred entry: 2.4.3.7

substrate_name: CMP-N-acetylneuraminate; Fetuin

enzyme: Transferred entry: 2.4.3.7

substrate: CMP-N-acetylneuraminate; Fetuin


organism: Bacillus subtilis

substrate_name: L-glutamate

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: L-glutamate


organism: Stenotrophomonas maltophilia

substrate_name: chorismate

proof_lines: second reaction step, cosubstrate NH3, pH 8, 25°C

substrate: chorismate


enzyme_display_name: hygromycin B 4-O-kinase

organism: Escherichia coli

substrate_name: ATP

assay_conditions_summary: cofactor ATP

proof_lines:
HPH
HPH and HPH5
HPH5

enzyme: hygromycin B 4-O-kinase

substrate: ATP


enzyme_display_name: receptor protein serine/threonine kinase

substrate_name: KKVLTQMGSPSIRCS(P)SVS

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

proof_lines: pH 7.5, 30°C, recombinant wild-type ALK5

substrate: KKVLTQMGSPSIRCS(P)SVS


proof_lines: pH 7.5, 30°C, recombinant mutant T204D ALK5, inhibition of autophosphorylation

enzyme: receptor protein serine/threonine kinase


enzyme_display_name: protein-histidine pros-kinase

proof_lines: in presence of activators: GTP or recombinant RAS protein

proof_lines: in absence of activators: GTP or recombinant RAS protein

enzyme: protein-histidine pros-kinase


enzyme_display_name: branched-chain-fatty-acid kinase

organism: Spirochaeta sp.

substrate_name: valerate

enzyme: branched-chain-fatty-acid kinase

substrate: valerate


organism: Unknown

substrate_name: carboxyphosphoenolpyruvate

substrate: carboxyphosphoenolpyruvate


organism: Streptomyces hygroscopicus


enzyme_display_name: propionate CoA-transferase

organism: Anaerotignum propionicum

substrate_name: propionate

enzyme: propionate CoA-transferase

substrate: propionate


assay_conditions_summary: pH 7; 298.15 K; cofactor CoA


organism: Cupriavidus necator

substrate_name: propionyl-CoA

assay_conditions_summary: pH 7.4; 303.15 K; cofactor CoA

substrate: propionyl-CoA


enzyme_display_name: pApA phosphodiesterase

organism: Streptococcus mitis

enzyme: pApA phosphodiesterase


organism: Streptococcus pneumoniae D39


enzyme_display_name: [protein ADP-ribosylarginine] hydrolase

substrate_name: ADP-ribosylarginine

assay_conditions_summary: cofactor ADP

enzyme: [protein ADP-ribosylarginine] hydrolase

substrate: ADP-ribosylarginine


organism: Meleagris gallopavo


substrate_name: ADP-ribosylarginine methyl ester

proof_lines: in presence of 15 mM magnesium and 5 mM DTT

substrate: ADP-ribosylarginine methyl ester


organism: Lysinibacillus sphaericus

proof_lines:
enzyme from spores
enzyme from sporulation medium
immobilized enzyme


proof_lines:
pH 7.3, 37°C, recombinant enzyme
pH 7.3, 37°C


enzyme_display_name: arginyltransferase

organism: Oryctolagus cuniculus

substrate_name: Unknown

enzyme: arginyltransferase

substrate: Unknown


organism: Saccharomyces cerevisiae

substrate_name: L-Arginyl-tRNA

substrate: L-Arginyl-tRNA


organism: Sus scrofa


enzyme_display_name: isopullulanase

organism: Aspergillus niger

substrate_name: pullulan

enzyme: isopullulanase

substrate: pullulan


substrate_name: panose

proof_lines:
mutant enzyme W402F
pH 3.5, 40°C, mutant enzyme W402F

substrate: panose


proof_lines:
wild-type enzyme
pH 3.5, 40°C, wild-type enzyme


enzyme_display_name: L-peptidase

organism: Foot-and-mouth disease virus

enzyme: L-peptidase


proof_lines: isoform Lbpro, pH and temperature not specified in the publication


enzyme_display_name: scytalidopepsin B

organism: Scytalidium lignicola

proof_lines: pH and temperature not specified in the publication

enzyme: scytalidopepsin B


enzyme_display_name: N-methyl-2-oxoglutaramate hydrolase

organism: Pseudomonas sp.

substrate_name: alpha-ketoglutarate

proof_lines: turnover number based on the molecular weight of 90000

enzyme: N-methyl-2-oxoglutaramate hydrolase

substrate: alpha-ketoglutarate


enzyme_display_name: arylalkyl acylamidase

organism: Pseudomonas putida

enzyme: arylalkyl acylamidase


enzyme_display_name: L-threonine aldolase

organism: Clostridium pasteurianum

substrate_name: L-threonine

enzyme: L-threonine aldolase

substrate: L-threonine


organism: Escherichia coli

substrate_name: L-allo-threonine

substrate: L-allo-threonine


enzyme_display_name: envelysin

organism: Paracentrotus lividus

substrate_name: dimethylated casein

enzyme: envelysin

substrate: dimethylated casein


organism: Strongylocentrotus purpuratus


enzyme_display_name: cyclohexanone monooxygenase

organism: Acidovorax sp. CHX100

substrate_name: cyclohexanone

assay_conditions_summary: cofactor NADPH

proof_lines: suspended cells, pH not specified in the publication, temperature not specified in the publication

proof_lines: biofilm, pH not specified in the publication, temperature not specified in the publication

proof_lines: purified enzyme, pH not specified in the publication, temperature not specified in the publication

enzyme: cyclohexanone monooxygenase

substrate: cyclohexanone


organism: Acinetobacter johnsonii

assay_conditions_summary: pH 8.5; 293.15 K; cofactor NADPH

proof_lines:
pH 8.5, 20°C, wild-type enzyme
25°C, pH 8.0, wild-type enzyme


organism: Acinetobacter sp.


substrate_name: NADPH

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

proof_lines:
wild-type, pH 7.7, 25°C
wild-type, pH 7.5, 25°C

substrate: NADPH


assay_conditions_summary: pH 8; cofactor NADPH

proof_lines: recombinant engineered enzyme expressed in Escherichia coli, pH 8.0, temperature not specified in the publication

proof_lines: recombinant engineered enzyme expressed in Pichia pastoris, pH 8.0, temperature not specified in the publication


enzyme_display_name: D-2-hydroxyacid dehydrogenase (NAD(+))

organism: Lactobacillus delbrueckii subsp. bulgaricus

substrate_name: phenylpyruvate

proof_lines: recombinant His-tagged enzyme, pH and temperature not specified in the publication

enzyme: D-2-hydroxyacid dehydrogenase (NAD(+))

substrate: phenylpyruvate


enzyme_display_name: ferredoxin--nitrite reductase

organism: Cyanidioschyzon merolae

substrate_name: nitrite

proof_lines: mutant G212S/L213T/Y214L/S217C/C220I/S221N, pH not specified in the publication, temperature not specified in the publication

enzyme: ferredoxin--nitrite reductase

substrate: nitrite


mutation_signature: truncated protein

proof_lines: truncated protein, residues 19-731, pH not specified in the publication, temperature not specified in the publication


proof_lines: truncated protein, residues 76-679, pH not specified in the publication, temperature not specified in the publication


organism: Nicotiana tabacum


organism: Rhodococcus sp. HI-31

proof_lines: mutant W492A, 0.1 mM cyclohexanone, 50 mM Tris-HCl buffer (pH 9.0), 25°C


proof_lines: wild-type, pH 7.5, temperature not specified in the publication

proof_lines: wild type enzyme, 0.1 mM cyclohexanone, 50 mM Tris-HCl buffer (pH 9.0), 25°C


proof_lines: mutant E91Q/D95H/A115V/R280Y/N431Y/Q439P/A455V/S534R, pH 7.5, temperature not specified in the publication


organism: Thermocrispum municipale

substrate_name: phenylacetone

substrate: phenylacetone


substrate_name: acetophenone

proof_lines:
pH 7.0, 25°C
Raw SKiD Env: Temp=25°C, pH=7

substrate: acetophenone


substrate_name: Cyclopentanone

substrate: Cyclopentanone


smiles: CCC(C)=O


organism: Lacticaseibacillus paracasei


assay_conditions_summary: pH 7.7; 298.15 K; cofactor NADPH


organism: Ilyonectria destructans

substrate_name: Cyclobutanone

substrate: Cyclobutanone


substrate_name: Cyclooctanone

substrate: Cyclooctanone


substrate_name: Cycloheptanone

substrate: Cycloheptanone


organism: Synechocystis sp. PCC 6803

proof_lines: cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication

proof_lines: cosubstrate Fdx2, pH 7.5, temperature not specified in the publication


enzyme_display_name: glutathionyl-hydroquinone reductase

substrate_name: glutathione

enzyme: glutathionyl-hydroquinone reductase

substrate: glutathione


enzyme_display_name: 1,3-beta-D-glucan phosphorylase

organism: Euglena gracilis var. saccharophila

substrate_name: laminaritetraose

enzyme: 1,3-beta-D-glucan phosphorylase

substrate: laminaritetraose


substrate_name: alpha-D-glucose

substrate: alpha-D-glucose


substrate_name: Laminarihexaose

substrate: Laminarihexaose


substrate_name: laminaribiose

substrate: laminaribiose


substrate_name: laminaritriose

substrate: laminaritriose


substrate_name: Laminaripentaose

substrate: Laminaripentaose


organism: Paenibacillus polymyxa

proof_lines: pH and temperature not specified in the publication


organism: Pyrococcus horikoshii

substrate_name: S-adenosyl-L-methionine

proof_lines:
at pH 8.5 and 37°C
Raw SKiD Env: Temp=37°C, pH=8.5

substrate: S-adenosyl-L-methionine


organism: Salinispora tropica

substrate_name: (R)-S-adenosyl-L-methionine

substrate: (R)-S-adenosyl-L-methionine


enzyme_display_name: selenocysteine lyase

organism: Arabidopsis thaliana

substrate_name: L-Selenocysteine; Reduced acceptor

enzyme: selenocysteine lyase

substrate: L-Selenocysteine; Reduced acceptor


organism: Escherichia coli


enzyme_display_name: assimilatory sulfite reductase (ferredoxin)

substrate_name: sulfite

substrate: sulfite


enzyme: assimilatory sulfite reductase (ferredoxin)


organism: Zea mays

substrate_name: Reduced ferredoxin; Sulfite

substrate: Reduced ferredoxin; Sulfite


organism: Eschscholzia californica

substrate_name: (R,S)-Stylopine

substrate: (R,S)-Stylopine


organism: Papaver somniferum

substrate_name: (R,S)-stylopine

proof_lines: varying concentration from 0.5 to 32 microM at a saturating concentration of 20 microM of S-adenosyl-L-methionine

proof_lines: varying concentration from 0.625 to 40 microM, at a saturating concentration of 50 microM of (R,S)-stylopine

substrate: (R,S)-stylopine


organism: Haloferax volcanii

substrate_name: L-selenocysteine

substrate: L-selenocysteine


organism: Leuconostoc mesenteroides


organism: Mus musculus


organism: Rattus norvegicus

substrate_name: Unknown

substrate: Unknown


organism: Nocardia globerula

substrate_name: H+; NADPH; O2; Cyclohexanone

proof_lines:
isozyme type 1
isozyme type 2

substrate: H+; NADPH; O2; Cyclohexanone


organism: Unknown

substrate_name: Thiane

substrate: Thiane


organism: Acinetobacter johnsonii

substrate_name: cyclohexanone

substrate: cyclohexanone


substrate_name: ferredoxin

substrate: ferredoxin


organism: Homo sapiens

substrate_name: GalNAc-alpha-Bn

substrate: GalNAc-alpha-Bn


organism: Acinetobacter calcoaceticus


proof_lines: pH 7.0, 25°C, mutant enzyme T56S/L244R/I491


proof_lines: pH 7.0, 25°C, mutant enzyme T56S/L435N/I491


organism: Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4)

substrate_name: nitrite

substrate: nitrite


organism: Escherichia coli (strain K12)

substrate_name: glutathione

proof_lines: kcat is 15, 14, 7.2 and 4.9 sec(-1) for the reduction of GS-MHQ, GS-HQ, GS-HHQ and GS-menadiol, respectively.

substrate: glutathione


substrate_name: cryptopine

proof_lines: kcat is 0.034 sec(-1) with cryptopine as substrate.

substrate: cryptopine


organism: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


organism: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)


organism: Salinispora arenicola (strain CNS-205)


organism: Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / JCM 13857 / NBRC 105044 / CNB-440)

substrate_name: (R)-S-adenosyl-L-methionine

substrate: (R)-S-adenosyl-L-methionine


organism: Streptomyces sp

substrate_name: S-adenosyl-L-methionine (for fluorinase activity)

proof_lines: kcat is 0.262 min(-1) for fluorinase activity (PubMed:24449539). kcat is 0.22 min(-1) for fluorinase activity (PubMed:27739177). kcat is 0.017 min(-1) for SAM hydrolase activity (PubMed:36996195). kcat is 0.008 min(-1) for chlorinase activi


organism: Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)


proof_lines: kcat is 30.6 sec(-1) with 1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) as substrate. kcat is 85.3 sec(-1) with 1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) as substrate with Mg(2+). kca


substrate_name: 1D-myo-inositol 1,4,5-trisphosphate

proof_lines: kcat is 6.5 sec(-1) with D-myo-inositol 1,4,5-trisphosphate.

substrate: 1D-myo-inositol 1,4,5-trisphosphate


organism: Bifidobacterium longum subsp. longum

proof_lines: kcat is 199 sec(-1) with alpha-D-Galp-(1->3)-alpha-L-Araf-OMe as substrate (PubMed:33674431). kcat is 12.1 sec(-1) with beta-L-Arap-(1->3)-alpha-L-Araf-OMe as substrate (PubMed:33674431).


organism: Enterococcus faecalis


organism: Synechocystis sp.

substrate_name: reduced ferredoxin

proof_lines: substrate Leptolyngbya boryana ferredoxin mutant E94Q/E95Q, pH 7.5, 25°C

substrate: reduced ferredoxin


proof_lines: substrate Leptolyngbya boryana ferredoxin mutant E31Q/E32Q/D36N, pH 7.5, 25°C


proof_lines: substrate Leptolyngbya boryana ferredoxin mutant D67N/D68N/D69N, pH 7.5, 25°C


proof_lines: substrate Leptolyngbya boryana ferredoxin, pH 7.5, 25°C

proof_lines: substrate maize ferredoxin, pH 7.5, 25°C


organism: Zea mays


organism: Salinispora arenicola


assay_conditions_summary: pH 7; buffer 30 mM potassium phosphate; cofactor NADP+

proof_lines: using E92Q/E30Q/D34N mutant ferredoxin, the reaction mixture contains regulator of fumarate and nitrate reduction (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM

proof_lines: using E92Q/E30Q/D34N mutant ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl


proof_lines: using E92Q/E93Q/E29Q/D34N mutant ferredoxin, the reaction mixture contains regulator of fumarate and nitrate reduction (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 2

proof_lines: using E92Q/E93Q/E29Q/D34N mutant ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl


proof_lines: using wild type ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl


assay_conditions_summary: pH 7; buffer 30-mM potassium phosphate; cofactor NADP+

proof_lines: using E92Q/E93Q mutant ferredoxin, the reaction mixture contains regulator of fumarate and nitrate reduction (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30-mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl

proof_lines: using E92Q/E93Q mutant ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl


proof_lines: pH 8.6, 25°C, recombinant and native enzyme


organism: Acinetobacter sp.

substrate_name: delta-thiovalerolactone

proof_lines: substrate inactivates enzyme after a few turnovers

substrate: delta-thiovalerolactone


organism: Exophiala jeanselmei

substrate_name: NADPH

substrate: NADPH


organism: Xanthobacter sp.


organism: Chlamydomonas reinhardtii

proof_lines: cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication

proof_lines: cosubstrate Fdx2, pH 7.5, temperature not specified in the publication


organism: Chlorolobion braunii

substrate_name: oxidized ferredoxin

substrate: oxidized ferredoxin


organism: Cyberlindnera jadinii

substrate_name: methyl viologen

substrate: methyl viologen


organism: Lactococcus cremoris

substrate_name: benzoylformate

substrate: benzoylformate


organism: Lactococcus lactis

assay_conditions_summary: pH 7; 310.15 K; cofactor NADH


proof_lines: pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2


organism: Haloferax mediterranei

substrate_name: NADH

assay_conditions_summary: pH 9; 325.15 K; buffer 0.1 M glycine-NaOH; cofactor NADH

proof_lines: with 2-oxobutyrate as cosubstrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C

proof_lines: in 0.1 M acetate buffer, pH 5.0, 4 M NaCl, at 52°C

proof_lines: with 2-oxoisocaproate as cosubstrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C

substrate: NADH


organism: Lacticaseibacillus casei

assay_conditions_summary: pH 7

proof_lines: pH 7.0, temperature not specified in the publication


organism: Lactiplantibacillus plantarum

substrate_name: phenylpyruvate

assay_conditions_summary: pH 8; 303.15 K; buffer 100 mM Tris-HC1

proof_lines: in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C

substrate: phenylpyruvate


organism: Limnospira platensis


organism: Scenedesmus fuscus


organism: Spinacia oleracea


organism: Cupriavidus necator


organism: Populus trichocarpa


organism: Saccharomyces cerevisiae


substrate_name: sulfite

proof_lines: 69K enzyme, reduced methyl viologen as electron donor

proof_lines: 63K enzyme, ferredoxin als electeron donor

substrate: sulfite


organism: Corydalis vaginans

substrate_name: (S)-Stylopine

substrate: (S)-Stylopine


organism: Papaver somniferum


organism: Sanguinaria canadensis


organism: Brassica rapa

proof_lines:
root enzyme
leaf enzyme


organism: Cyanidioschyzon merolae


organism: Methanocaldococcus jannaschii

substrate_name: reduced coenzyme F420

proof_lines: at a fixed sulfite concentration of 0.29 mM and within a reduced coenzyme F420 concentration range of 0.004-0.06 mM

proof_lines: within a sulfite concentration range of 0.0014-0.3 mM and with a fixed reduced coenzyme F420 concentration of 0.04 mM

substrate: reduced coenzyme F420


organism: Acacia verek

substrate_name: Laminarin

proof_lines:
reaction with glucose 1-phosphate
phosphorolytic reaction

substrate: Laminarin


organism: Ochromonas danica

substrate_name: laminaritriose

proof_lines:
with phosphate, pH 7.5, 30°C
with laminaritriose, pH 7.5, 30°C

substrate: laminaritriose


organism: Poterioochromonas malhamensis


organism: Thermosipho africanus

substrate_name: xylose

substrate: xylose


organism: Salinispora arenicola

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


organism: Arabidopsis thaliana

substrate_name: L-selenocysteine

substrate: L-selenocysteine


organism: Citrobacter freundii

substrate_name: beta-chloro-L-alanine

substrate: beta-chloro-L-alanine


organism: Escherichia coli


organism: Homo sapiens


enzyme_display_name: L(+)-tartrate dehydratase

substrate_name: (R,R)-Tartrate

enzyme: L(+)-tartrate dehydratase

substrate: (R,R)-Tartrate


organism: Pseudomonas putida

proof_lines:
elimination of air
in the presence of air
relatively anaerobic conditions


enzyme_display_name: dTDP-4-dehydrorhamnose 3,5-epimerase

organism: Salmonella enterica

enzyme: dTDP-4-dehydrorhamnose 3,5-epimerase


enzyme_display_name: methyl farnesoate epoxidase

organism: Locusta migratoria

substrate_name: methyl (2E,6E)-farnesoate

assay_conditions_summary: pH 8; 303.15 K; cofactor NADPH

enzyme: methyl farnesoate epoxidase

substrate: methyl (2E,6E)-farnesoate


organism: Oryctolagus cuniculus


organism: Rattus norvegicus


organism: Sus scrofa


organism: Aneurinibacillus thermoaerophilus


organism: Salmonella typhimurium


organism: Streptococcus suis


proof_lines:
pH 7.0, 25°C
native


organism: Pseudomonas fluorescens

substrate_name: H+; NADPH; O2; Cyclohexanone

substrate: H+; NADPH; O2; Cyclohexanone


organism: Amycolatopsis orientalis


organism: Streptococcus entericus


organism: Diploptera punctata

substrate_name: Methyl (2E,6E)-farnesoate; O2; [reduced NADPH-hemoprotein reductase]


substrate_name: S-Adenosyl-L-methionine; Uridine(2552) in 23S rRNA


organism: Klebsiella pneumoniae

proof_lines: mutant enzyme Q88E, pH and temperature not specified in the publication


proof_lines: wild-type enzyme, pH and temperature not specified in the publication


enzyme_display_name: arsenate reductase (cytochrome c)

organism: Hydrogenophaga sp.

substrate_name: arsenite

proof_lines: pH 5.5, temperature not specified in the publication

enzyme: arsenate reductase (cytochrome c)

substrate: arsenite


organism: Pseudorhizobium banfieldiae


enzyme_display_name: hydroperoxy fatty acid reductase

organism: Synechocystis sp.

substrate_name: NADPH

assay_conditions_summary: pH 8.2; 310.15 K; cofactor NADPH

proof_lines:
isoform Gpx-1, at pH 8.2 and 37°C
isoform Gpx-2, at pH 8.2 and 37°C

enzyme: hydroperoxy fatty acid reductase

substrate: NADPH


enzyme_display_name: sulfur oxygenase/reductase

organism: Acidianus ambivalens

substrate_name: sulfur

proof_lines: pH 7.2, 65°C, reductase reaction (formation of sulfite plus thiosulfate)

proof_lines: pH 7.2, 65°C, oxygenase reaction (formation of hydrogen sulfide)

proof_lines: pH 7.2, 65°C, reductase reaction (formation of sulphite plus thiosulfate)

enzyme: sulfur oxygenase/reductase

substrate: sulfur


enzyme_display_name: glycine betaine monooxygenase

organism: Chromohalobacter israelensis

substrate_name: NADH

assay_conditions_summary: pH 7; 298.15 K; cofactor NADPH

enzyme: glycine betaine monooxygenase

substrate: NADH


enzyme_display_name: (R)-limonene 6-monooxygenase

organism: Carum carvi

substrate_name: (+)-limonene

proof_lines:
annual caraway
biannual caraway

enzyme: (R)-limonene 6-monooxygenase

substrate: (+)-limonene


enzyme_display_name: chlordecone reductase

organism: Meriones unguiculatus

enzyme: chlordecone reductase


organism: Halothiobacillus neapolitanus

substrate_name: Unknown

substrate: Unknown


substrate_name: sulfur (at 65 degrees Celsius, with the oxygenase reaction)


enzyme_display_name: trehalose O-mycolyltransferase

organism: Mycobacterium leprae

substrate_name: alpha,alpha-trehalose 6-monomycolate

proof_lines: wild-type, short-chain trehalose monomycolate is used as a substrate, Vmax: 3.83 nmol/min/nmol

enzyme: trehalose O-mycolyltransferase

substrate: alpha,alpha-trehalose 6-monomycolate


proof_lines: mutant L130S, short-chain trehalose monomycolate is used as a substrate, Vmax: 4.27 nmol/min/nmol


enzyme_display_name: ferric-chelate reductase [NAD(P)H]

organism: Archaeoglobus fulgidus

assay_conditions_summary: pH 7; 358.15 K; cofactor NADPH

proof_lines:
pH 7.0, 85°C
pH 7.0, 85°C, cosubstrate: NADPH

enzyme: ferric-chelate reductase [NAD(P)H]


enzyme_display_name: NAD(P)H sulfur oxidoreductase (CoA-dependent)

organism: Pyrococcus furiosus


organism: Thermococcus litoralis

substrate_name: polysulfide(n)

assay_conditions_summary: pH 8.9; 338.15 K; cofactor NADPH

enzyme: NAD(P)H sulfur oxidoreductase (CoA-dependent)

substrate: polysulfide(n)


organism: Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)

substrate_name: NADH (with Fe(+3) as acceptor)

substrate: NADH (with Fe(+3) as acceptor)


organism: Vibrio vulnificus


enzyme_display_name: methylarsonate reductase

organism: Caenorhabditis elegans

enzyme: methylarsonate reductase


enzyme_display_name: lipoyl(octanoyl) transferase

organism: Escherichia coli (strain K12)

substrate_name: octanoyl-ACP

enzyme: lipoyl(octanoyl) transferase

substrate: octanoyl-ACP


organism: Oryctolagus cuniculus

substrate_name: monomethylarsonate

substrate: monomethylarsonate


organism: Mycolicibacterium smegmatis


organism: Escherichia coli

substrate_name: octanoyl-[acyl-carrier protein]

substrate: octanoyl-[acyl-carrier protein]


enzyme_display_name: sphingoid base N-stearoyltransferase

organism: Homo sapiens

substrate_name: dihydrosphingosine

proof_lines: pH and temperature not specified in the publication

enzyme: sphingoid base N-stearoyltransferase

substrate: dihydrosphingosine


organism: Mus musculus

substrate_name: stearoyl-CoA

assay_conditions_summary: pH 7.4; 298.15 K; cofactor CoA

substrate: stearoyl-CoA


enzyme_display_name: zeatin O-beta-D-xylosyltransferase

organism: Phaseolus vulgaris

substrate_name: UDPxylose; Zeatin

enzyme: zeatin O-beta-D-xylosyltransferase

substrate: UDPxylose; Zeatin


organism: Sus scrofa

substrate_name: pyridylaminated lacto-N-neotetraose

substrate: pyridylaminated lacto-N-neotetraose


organism: Niallia circulans

substrate_name: dextran T-40

substrate: dextran T-40


organism: Thermoanaerobacter thermocopriae

substrate_name: Dextran

proof_lines: wild-type, K0.5 value, Hill coefficient 0.76, pH 6.0, 55°C

substrate: Dextran


proof_lines: variant lacks the long isomaltooligosaccharide-forming domain, K0.5 value, Hill coefficient 0.73 pH 6.0, 55°C

proof_lines: variant lacks both the long isomaltooligosaccharide-forming domain and the carbohydrate-binding module, K0.5 value, Hill coefficient 0.26, pH 6.0, 55°C


substrate_name: UDP-xylose

substrate: UDP-xylose


enzyme_display_name: 3-alpha-mycarosylerythronolide B desosaminyl transferase

organism: Saccharopolyspora erythraea

substrate_name: 3-alpha-mycarosylerythronolide B

proof_lines: pH and temperature not specified in the publication, 0.05 mM TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose

proof_lines: pH 8.0, 25°C, in the presence og 0.001 mM EryC and 1 mM of each substrate, more than 300 turnovers are observed in 2.5 min, indicating that the kcat must be greater than 100/min. This is one of the highest turnover numbers reported for an a

proof_lines: pH and temperature not specified in the publication, 0.05 mM dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose

substrate: 3-alpha-mycarosylerythronolide B


substrate_name: UDP-alpha-D-glucose

sequence_source: UniProtKB B5L3F2 identity anchor

proof_lines: pH 7.0, 37°C, assayed with bacterial membranes

substrate: UDP-alpha-D-glucose


sequence_source: UniProtKB Q077R2 identity anchor


organism: Saccharomyces cerevisiae

substrate_name: 2-deoxy-D-glucose 6-phosphate

proof_lines:
isoenzyme DogR1p
isoenzyme DogR2p

substrate: 2-deoxy-D-glucose 6-phosphate


enzyme_display_name: signal peptidase II

organism: Unknown

substrate_name: prolipoprotein

enzyme: signal peptidase II

substrate: prolipoprotein


enzyme_display_name: 2,4'-dihydroxyacetophenone dioxygenase

organism: Burkholderia sp.

enzyme: 2,4'-dihydroxyacetophenone dioxygenase


organism: Glycine max


enzyme_display_name: formyltetrahydrofolate deformylase

enzyme: formyltetrahydrofolate deformylase


enzyme_display_name: phenylacetaldehyde synthase

organism: Petunia x hybrida

substrate_name: L-phenylalanine

enzyme: phenylacetaldehyde synthase

substrate: L-phenylalanine


enzyme_display_name: inositol-tetrakisphosphate 5-kinase

organism: Arabidopsis thaliana

enzyme: inositol-tetrakisphosphate 5-kinase


assay_conditions_summary: cofactor ATP


organism: Rattus norvegicus


enzyme_display_name: deoxyribokinase

organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

enzyme: deoxyribokinase


enzyme_display_name: succinyl-CoA hydrolase

substrate_name: succinyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: succinyl-CoA hydrolase

substrate: succinyl-CoA


substrate_name: decanoyl-CoA

substrate: decanoyl-CoA


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

substrate_name: inhibitor of cysteine peptidase

substrate: inhibitor of cysteine peptidase


organism: Staphylococcus aureus (strain USA300)

substrate_name: P.aeruginosa inhibitor of cysteine peptidase


enzyme_display_name: N(alpha)-acyl-L-glutamine aminoacylase

organism: Corynebacterium striatum

enzyme: N(alpha)-acyl-L-glutamine aminoacylase


enzyme_display_name: glycine betaine monooxygenase

organism: Chromohalobacter israelensis (strain ATCC BAA-138 / DSM 3043 / CIP 106854 / NCIMB 13768 / 1H11)

substrate_name: NADH (for flavin reductase activity, with FAD as cosubstrate)

assay_conditions_summary: cofactor NADH

proof_lines: kcat is 0.90 sec(-1) with NADH as substrate (for flavin reductase activity, with FAD as cosubstrate). kcat is 0.35 sec(-1) with NADPH as substrate (for flavin reductase activity, with FAD as cosubstrate). kcat is 4.25 sec(-1) with FAD as su

enzyme: glycine betaine monooxygenase


substrate_name: glycine betaine

proof_lines: kcat is 0.65 sec(-1) with glycine betaine as substrate.

substrate: glycine betaine


enzyme_display_name: (R)-limonene 6-monooxygenase

organism: Thymus vulgaris

substrate_name: gamma-terpinene

proof_lines: kcat is 1.24 sec(-1) with gamma-terpinene as substrate (Ref.1). kcat is 0.08 sec(-1) with (4R)-limonene as substrate (Ref.1). kcat is 0.15 sec(-1) with (4S)-limonene as substrate (Ref.1).

enzyme: (R)-limonene 6-monooxygenase

substrate: gamma-terpinene


enzyme_display_name: chlordecone reductase

organism: Homo sapiens

proof_lines: kcat is 1.99 min(-1) for 17beta-hydroxy-5alpha-androstan-3-one reduction. kcat is 1.79 min(-1) for 5alpha-androstan-3,17-dione reduction. kcat is 2.1 min(-1) for 5alpha-androstane-3alpha,17beta-diol oxydation. kcat is 1.39.1 min(-1) for 3al

enzyme: chlordecone reductase


organism: Macaca fuscata fuscata

proof_lines: kcat is 4.9 min(-1) for 5-beta-pregnan-20-alpha-ol-3-one as substrate. kcat is 4.9 min(-1) for 3alpha-hydroxy-5alpha-androstan-17-one as substrate. kcat is 23 min(-1) for 5alpha-pregnane-3alpha,21-diol-20-one.


enzyme_display_name: NAD(P)H sulfur oxidoreductase (CoA-dependent)

organism: Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)

substrate_name: NADH

assay_conditions_summary: cofactor NADPH

proof_lines: kcat is 68 sec(-1) for NADH oxidase activity. kcat is 50 sec(-1) for NADPH oxidase activity.

substrate: NADH


enzyme_display_name: trehalose O-mycolyltransferase

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: C26:0-CoA (with 1,2-dipalmitin as the acyl acceptor and at 37 degrees Celsius)

enzyme: trehalose O-mycolyltransferase


organism: Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)

enzyme: 3-alpha-mycarosylerythronolide B desosaminyl transferase


organism: Mus musculus

substrate_name: acetyl-CoA

proof_lines: In summary, KM for medium- to long-chain acyl CoAs is in the order 1.4-6.7 uM, and with short-chain acyl CoAs range from 8 to 30 uM. KM for bile acid-CoA esters is in the range 6-15 uM.

substrate: acetyl-CoA


enzyme_display_name: thiamine phosphate phosphatase

substrate_name: thiamine monophosphate

proof_lines: kcat is 29.9 sec(-1) for thiamine monophosphate.

enzyme: thiamine phosphate phosphatase

substrate: thiamine monophosphate


proof_lines: kcat is 13.5 sec(-1) for thiamine monophosphate.


organism: Escherichia coli (strain K12)

substrate_name: 2-deoxyglucose-6-P (with manganese ions as cofactor and at pH 9)

assay_conditions_summary: pH 9

proof_lines: kcat is 33 sec(-1) with 2-deoxyglucose-6-P as substrate. kcat is 11 sec(-1) with mannose-6-P as substrate. kcat is 9.4 sec(-1) with 2-deoxyribose-5-P as substrate. kcat is 2.8 sec(-1) with ribose-5-P as substrate. kcat is 25 sec(-1) with gl


enzyme_display_name: dolichyldiphosphatase

substrate_name: dolichyl diphosphate

enzyme: dolichyldiphosphatase

substrate: dolichyl diphosphate


organism: Burkholderia sp. AZ11


enzyme_display_name: 7-deoxycylindrospermopsin hydroxylase

organism: Oscillatoria sp. PCC 7926

proof_lines: pH and temperature not specified in the publication

enzyme: 7-deoxycylindrospermopsin hydroxylase


enzyme_display_name: (S)-3-hydroxyacid-ester dehydrogenase

organism: Klebsiella pneumoniae

substrate_name: benzyl 2-methyl-3-oxobutanoate

enzyme: (S)-3-hydroxyacid-ester dehydrogenase

substrate: benzyl 2-methyl-3-oxobutanoate


organism: Saccharomyces cerevisiae

substrate_name: ethyl 3-oxobutyrate

substrate: ethyl 3-oxobutyrate


enzyme_display_name: arsenate reductase (azurin)

organism: Alcaligenes faecalis

substrate_name: azurin

enzyme: arsenate reductase (azurin)

substrate: azurin


organism: Arthrobacter sp.

substrate_name: arsenite

assay_conditions_summary: pH 6

substrate: arsenite


organism: Chrysiogenes arsenatis

substrate_name: arsenate

substrate: arsenate


enzyme_display_name: bis-gamma-glutamylcystine reductase

organism: Halobacterium salinarum

substrate_name: FAD

enzyme: bis-gamma-glutamylcystine reductase

substrate: FAD


enzyme_display_name: isoorientin 3'-O-methyltransferase

organism: Silene latifolia subsp. alba

substrate_name: caffeic acid

proof_lines: second substrate isoorientin 2-O-rhamnoside

enzyme: isoorientin 3'-O-methyltransferase

substrate: caffeic acid


organism: Malonomonas rubra

substrate_name: malonate

assay_conditions_summary: pH 6; cofactor CoA

substrate: malonate


enzyme_display_name: formaldehyde transketolase

organism: Acinetobacter sp.

substrate_name: formaldehyde

enzyme: formaldehyde transketolase

substrate: formaldehyde


organism: [Candida] boidinii

substrate_name: D-xylulose 5-phosphate

substrate: D-xylulose 5-phosphate


enzyme_display_name: noranthrone synthase

organism: Aspergillus parasiticus

substrate_name: hexanoyl-[acyl-carrier protein]

enzyme: noranthrone synthase

substrate: hexanoyl-[acyl-carrier protein]


enzyme_display_name: monoterpenol O-acetyltransferase

organism: Mentha x piperita

substrate_name: (-)-menthol

proof_lines:
leaf enzyme
flower head enzyme

enzyme: monoterpenol O-acetyltransferase

substrate: (-)-menthol


enzyme_display_name: heme oxygenase (biliverdin-IX-beta and delta-forming)

organism: Pseudomonas aeruginosa

substrate_name: heme

substrate: heme


organism: Pseudomonas fluorescens

proof_lines: PigA, pH and temperature not specified in the publication

enzyme: heme oxygenase (biliverdin-IX-beta and delta-forming)


substrate_name: bis-gamma-glutamylcystine

proof_lines: pH 6.7, temperature not specified in the publication

substrate: bis-gamma-glutamylcystine


enzyme_display_name: farnesylcysteine lyase

organism: Arabidopsis thaliana

enzyme: farnesylcysteine lyase


organism: Staphylococcus aureus

substrate_name: 2-methyladenine2503 in 23S rRNA fragment (2447-2625)


enzyme_display_name: U6 snRNA m(6)A methyltransferase

organism: Homo sapiens

substrate_name: S-adenosyl-L-methionine

proof_lines: with U6 snRNA as cosubstrate, in 10 mM HEPES, 150 mM KCl, 10 mM MgCl2, and 5 mM tris(2-carboxyethyl)phosphine, at pH 7.5 and 37°C

proof_lines: N-terminal methyltransferase domain, at pH 8.0 and 37°C

proof_lines: Km above 0.4 mM, with methionine adenosine transferase 2A mRNA hairpin 1 as cosubstrate, in 10 mM HEPES, 150 mM KCl, 10 mM MgCl2, and 5 mM tris(2-carboxyethyl)phosphine, at pH 7.5 and 37°C

enzyme: U6 snRNA m(6)A methyltransferase

substrate: S-adenosyl-L-methionine


substrate_name: recombinant nucleosome

proof_lines: cosubstrate chicken nucleosome, pH 9.0, 23°C

proof_lines: cosubstrate recombinant nucleosome, pH 9.0, 23°C

substrate: recombinant nucleosome


substrate_name: L-malate

substrate: L-malate


enzyme_display_name: chlorophyll synthase

organism: Synechocystis sp. PCC 6803

substrate_name: chlorophyllide a

proof_lines: pH 7.6, 30°C, recombinant wild-type enzyme

enzyme: chlorophyll synthase

substrate: chlorophyllide a


proof_lines: pH 7.6, 30°C, recombinant enzyme mutant I44F


organism: Pyrococcus horikoshii

substrate_name: GTP

substrate: GTP


enzyme_display_name: ceramide phosphoethanolamine synthase

organism: Mus musculus

enzyme: ceramide phosphoethanolamine synthase


enzyme_display_name: Transferred entry: 3.6.1.71

proof_lines: nicked DNA substrate, pH 7.5, 25-37°C, recombinant His-tagged wild-type enzyme

proof_lines: blunt DNA substrate, pH 7.5, 25-37°C, recombinant His-tagged wild-type enzyme

enzyme: Transferred entry: 3.6.1.71


enzyme_display_name: alpha-mannosidase

organism: Rattus norvegicus

substrate_name: H2O; p-Nitrophenyl-alpha-D-mannoside

enzyme: alpha-mannosidase

substrate: H2O; p-Nitrophenyl-alpha-D-mannoside


substrate_name: Unknown

substrate: Unknown


substrate_name: Succinyl-YVAD-p-nitroanilide; H2O

substrate: Succinyl-YVAD-p-nitroanilide; H2O


enzyme_display_name: neprilysin

proof_lines:
pH 6.5, 37°C, mutant NEPF563I
pH 6.5, 37°C, mutant NEPS546A
pH 6.5, 37°C, mutant NEPS546T

enzyme: neprilysin


substrate_name: amyloid beta peptide1-40

proof_lines: mutant G399V, pH and temperature not specified in the publication

substrate: amyloid beta peptide1-40


proof_lines: wild-type enzyme, pH and temperature not specified in the publication

proof_lines: pH 6.5, 37°C, wild-type enzyme and mutant NEPF563V


proof_lines: mutant G714K, pH and temperature not specified in the publication


proof_lines: mutant G399V/G714K, pH and temperature not specified in the publication


proof_lines: pH 7.5, 37°C, in presence of activating K49-P1-20

proof_lines: pH and temperature not specified in the publication


enzyme_display_name: leucine transaminase

organism: Glycine max

substrate_name: L-leucine

proof_lines:
isozyme LAT I, pH 9, 37°C
isozyme LAT II, pH 9, 37°C

enzyme: leucine transaminase

substrate: L-leucine


enzyme_display_name: cysteine-conjugate transaminase

proof_lines:
isoenzyme CAT-IIA, pH 7.0, 35°C
isoenzyme CAT-IIB, pH 7.0, 35°C
isoenzyme CAT-I, pH 7.0, 35°C

enzyme: cysteine-conjugate transaminase


enzyme_display_name: thermolysin

organism: Bacillus thermoproteolyticus

substrate_name: Z-Phe-Leu-Ala-OH; H2O

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

proof_lines: pH 8.0, 70°C, recombinant enzyme, autoactivation, without radiofrequency

proof_lines: pH 8.0, 70°C, recombinant enzyme, autoactivation, with radiofrequency

source_databases_merged: sabio_rk brenda skid

enzyme: thermolysin

substrate: Z-Phe-Leu-Ala-OH; H2O


proof_lines: purified recombinant mutant N116A, pH 7.5, 25°C


proof_lines: purified recombinant mutant N116D, pH 7.5, 25°C


proof_lines: purified recombinant mutant N116Q, pH 7.5, 25°C


proof_lines: purified recombinant wild-type enzyme, pH 7.5, 25°C


proof_lines: purified recombinant mutant N116T, pH 7.5, 25°C


enzyme_display_name: arabinose-5-phosphate isomerase

organism: Bacteroides fragilis

substrate_name: D-ribulose 5-phosphate

proof_lines:
pH 8.5, 37°C
Raw SKiD Env: Temp=37°C, pH=8.5

enzyme: arabinose-5-phosphate isomerase

substrate: D-ribulose 5-phosphate


organism: Clostridium tetani

substrate_name: D-arabinose 5-phosphate

substrate: D-arabinose 5-phosphate


organism: Escherichia coli


organism: Francisella tularensis

substrate_name: D-Arabinose 5-phosphate

substrate: D-Arabinose 5-phosphate


substrate_name: N-carbobenzoxy-L-Asp-L-Phe methyl ester

assay_conditions_summary: pH 7.5; 298.15 K; buffer 40 mM HEPES-NaOH

proof_lines: mutant enzyme S53D/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme S53D/G8C/N60C/S65P, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

substrate: N-carbobenzoxy-L-Asp-L-Phe methyl ester


proof_lines: mutant enzyme L155A/G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme L155A/G8C/N60C/S65P, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme L155A/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


proof_lines: mutant enzyme S53D/L155A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme S53D/L155A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme S53D/L155A, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


substrate_name: L-phenylalanine methyl ester

proof_lines: mutant enzyme D150E, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme D150E, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

substrate: L-phenylalanine methyl ester


substrate_name: N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide

proof_lines: mutant enzyme S53D, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme S53D, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme S53D, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

substrate: N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide


proof_lines: mutant enzyme S53D/L155A/G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme S53D/L155A/G8C/N60C/S65P, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme S53D/L155A/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


proof_lines: mutant enzyme L144S, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme L144S, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


proof_lines: mutant enzyme L155A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme L155A, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


proof_lines: pH 7.5, 25°C, recombinant mutants Q128K and Q225R


proof_lines: mutant enzyme D150E/I168A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


proof_lines: mutant enzyme L144S/D150E, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme L144S/D150E, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme L144S/D150E, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


proof_lines: mutant enzyme L144S/D150E/S53D, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme L144S/D150E/S53D, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme L144S/D150E/S53D, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


proof_lines: mutant enzyme I168A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme I168A, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


substrate_name: p-nitrophenyl-alpha-D-mannopyranoside (pNP-Man) (at 37 degrees Celsius and at pH 4.5)


enzyme_display_name: cyanide hydratase

organism: Aspergillus niger

substrate_name: cyanide

enzyme: cyanide hydratase

substrate: cyanide


organism: Fusarium solani


proof_lines: pH 7.5, 25°C, recombinant mutants Q128E and Q225E


organism: Bacillus sp. (in: firmicutes)

substrate_name: N-benzyloxycarbonyl-Gly-L-Leu amide

proof_lines: recombinant mutant L155S/I156V, pH 7.0, 37°C

substrate: N-benzyloxycarbonyl-Gly-L-Leu amide


proof_lines: recombinant mutant L155A/I156N, pH 7.0, 37°C


proof_lines: recombinant mutant L155S/I156N, pH 7.0, 37°C


proof_lines: recombinant mutant L155A/I156V, pH 7.0, 37°C


proof_lines: recombinant wild-type enzyme, pH 7.0, 37°C


organism: Oryctolagus cuniculus


substrate_name: leucine enkephalin

substrate: leucine enkephalin


proof_lines: pH 7.0, 25°C, recombinant mutant G8C/N60C/S65P/L144S


assay_conditions_summary: pH 7; 298.15 K; buffer 40 mM HEPES-NaOH

proof_lines: pH 7.0, 25°C, recombinant mutant G8C/N60C/S65P

proof_lines: mutant enzyme G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme G8C/N60C/S65P, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


enzyme_display_name: dipeptidase E

organism: Salmonella enterica subsp. enterica serovar Typhimurium

enzyme: dipeptidase E


enzyme_display_name: 2-aminobenzenesulfonate 2,3-dioxygenase

organism: Alcaligenes sp.

substrate_name: benzenesulfonate

enzyme: 2-aminobenzenesulfonate 2,3-dioxygenase

substrate: benzenesulfonate


enzyme_display_name: aklavinone 12-hydroxylase

organism: Streptomyces purpurascens

substrate_name: NADPH

proof_lines: pH and temperature not specified in the publication

enzyme: aklavinone 12-hydroxylase

substrate: NADPH


enzyme_display_name: ferruginol synthase

organism: Salvia rosmarinus

enzyme: ferruginol synthase


enzyme_display_name: dimethylglycine N-methyltransferase

organism: Aphanothece halophytica

substrate_name: S-adenosyl-L-methionine

enzyme: dimethylglycine N-methyltransferase

substrate: S-adenosyl-L-methionine


organism: Methanosarcina acetivorans

substrate_name: methylcob(III)alamin

proof_lines: at pH 7.2, temperature not specified in the publication

substrate: methylcob(III)alamin


enzyme_display_name: anthranilate N-benzoyltransferase

organism: Dianthus caryophyllus

substrate_name: benzoyl-CoA

enzyme: anthranilate N-benzoyltransferase

substrate: benzoyl-CoA


substrate_name: NAD (at pH 7.5)

substrate: NAD (at pH 7.5)


organism: Aspergillus parasiticus (strain ATCC 56775 / NRRL 5862 / SRRC 143 / SU-1)

substrate_name: demethylsterigmatocystin

substrate: demethylsterigmatocystin


enzyme_display_name: anthranilate N-methyltransferase

organism: Ruta graveolens

substrate_name: anthranilate

enzyme: anthranilate N-methyltransferase

substrate: anthranilate


organism: Parasynechococcus marenigrum (strain WH8102)

substrate_name: dimethylglycine (at pH 8.5 and at 37 degrees Celsius)


enzyme_display_name: acetyl-S-ACP:malonate ACP transferase

organism: Malonomonas rubra

substrate_name: malonate

enzyme: acetyl-S-ACP:malonate ACP transferase

substrate: malonate


organism: Petrotoga mobilis (strain DSM 10674 / SJ95)

substrate_name: glucosyl-3-phosphoglycerate (at 60 degrees Celsius)


enzyme_display_name: crocetin glucosyltransferase

organism: Gardenia jasminoides

substrate_name: crocetin

enzyme: crocetin glucosyltransferase

substrate: crocetin


organism: Acinetobacter calcoaceticus

substrate_name: malonyl-CoA

substrate: malonyl-CoA


organism: Klebsiella pneumoniae

assay_conditions_summary: pH 7; cofactor CoA


organism: Pseudomonas aeruginosa

proof_lines: in 50 mM HEPES (pH 8.0), 2.5 mM dithiothreitol, and 2 mM MgCl2, at 30°C


organism: Pseudomonas aeruginosa PAO1


organism: Candida maltosa

substrate_name: L-lysine

assay_conditions_summary: pH 8.1; 310.15 K; cofactor CoA

substrate: L-lysine


organism: Saccharomyces cerevisiae

substrate_name: L-thialysine

assay_conditions_summary: pH 8.5; 303.15 K; cofactor CoA

substrate: L-thialysine


enzyme_display_name: phenylpropanoylacetyl-CoA synthase

organism: Curcuma longa

substrate_name: feruloyl-CoA

assay_conditions_summary: pH 7.5; 310.15 K; cofactor CoA

enzyme: phenylpropanoylacetyl-CoA synthase

substrate: feruloyl-CoA


organism: Raphanus sativus


enzyme_display_name: isovitexin beta-glucosyltransferase

organism: Silene latifolia subsp. alba

substrate_name: UDPglucose

proof_lines:
reaction with isovitexin
reaction with isoorientin

enzyme: isovitexin beta-glucosyltransferase

substrate: UDPglucose


organism: Homo sapiens

substrate_name: UDP-galactose

substrate: UDP-galactose


organism: Notamacropus eugenii

substrate_name: lactose

substrate: lactose


enzyme_display_name: lipid-A-disaccharide synthase

organism: Escherichia coli

enzyme: lipid-A-disaccharide synthase


organism: Aspergillus niger

substrate_name: maltotriose

substrate: maltotriose


organism: Mycolicibacterium smegmatis

substrate_name: methyl-alpha-D-mannoside

substrate: methyl-alpha-D-mannoside


organism: Crocus sativus


enzyme_display_name: alpha-(1->3) branching sucrase

organism: Apilactobacillus kunkeei

substrate_name: sucrose

enzyme: alpha-(1->3) branching sucrase

substrate: sucrose


organism: Leuconostoc citreum

proof_lines:
no cosubstrate, pH 5.8, 30°C
cosubstrate dextran, pH 5.8, 30°C
pH 5.8, 30°C


enzyme_display_name: glucomannan 4-beta-mannosyltransferase

organism: Lathyrus oleraceus

substrate_name: GDP-D-mannose

enzyme: glucomannan 4-beta-mannosyltransferase

substrate: GDP-D-mannose


organism: Pinus sylvestris


organism: Vigna radiata var. radiata


organism: Aspergillus fumigatus

substrate_name: dimethylallyl diphosphate

substrate: dimethylallyl diphosphate


enzyme_display_name: leucine transaminase

organism: Rattus norvegicus

enzyme: leucine transaminase


enzyme_display_name: chlorophyll synthase

organism: Synechocystis sp. PCC 6803

substrate_name: geranylgeranyl diphosphate

assay_conditions_summary: pH 7.6; 303.15 K; buffer 120 mM potassium acetate; cofactor ATP

proof_lines: lysate mixed into reaction buffer (120 mM potassium acetate, 10 mM magnesium acetate, 50 mM HEPES-KOH, pH 7.6, 14 mM 2-mercaptoethanol, 10% glycerol, and 0.5 mM ATP), 30°C

proof_lines: in the presence of sufficient geranylgeranyl diphosphate as substrate, lysate mixed into reaction buffer (120 mM potassium acetate, 10 mM magnesium acetate, 50 mM HEPES-KOH, pH 7.6, 14 mM 2-mercaptoethanol, 10% glycerol, and 0.5 mM ATP), 30

enzyme: chlorophyll synthase

substrate: geranylgeranyl diphosphate


enzyme_display_name: AMP--thymidine kinase

organism: Asplenium nidus

substrate_name: AMP

enzyme: AMP--thymidine kinase

substrate: AMP


organism: Herpes simplex virus

substrate_name: thymidine

substrate: thymidine


enzyme_display_name: deoxyribokinase

organism: Salmonella enterica subsp. enterica serovar Typhi

enzyme: deoxyribokinase


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: ATP

assay_conditions_summary: pH 7.5; cofactor ATP

proof_lines: pH 7.5, temperature not specified in the publication

substrate: ATP


organism: Saccharomyces cerevisiae

substrate_name: phosphate

substrate: phosphate


enzyme_display_name: erythritol kinase (D-erythritol 4-phosphate-forming)

organism: Acidipropionibacterium acidipropionici

assay_conditions_summary: pH 8.6; 301.15 K; cofactor ATP


organism: Brucella abortus

assay_conditions_summary: pH 8; 298.15 K; cofactor ATP

proof_lines: pH 8.0, 25°C, recombinant erythritol kinase

enzyme: erythritol kinase (D-erythritol 4-phosphate-forming)


proof_lines:
pH 7.8, 37°C, submandibular gland
pH 7.8, 37°C, parotid gland
pH 7.8, 37°C


organism: Gallus gallus

substrate_name: UDP-N-acetylgalactosamine 4-sulfate

substrate: UDP-N-acetylgalactosamine 4-sulfate


organism: Homo sapiens

substrate_name: dermatan

proof_lines: pH 6, 37°C, Km value depends on number of disaccharides of the receptor

substrate: dermatan


enzyme_display_name: tropinesterase

organism: Oryctolagus cuniculus

substrate_name: atropine

proof_lines:
enzyme form Ef IX
enzyme form Ef VII

enzyme: tropinesterase

substrate: atropine


enzyme_display_name: succinyl-CoA hydrolase

organism: Mus musculus

substrate_name: succinyl-CoA

enzyme: succinyl-CoA hydrolase

substrate: succinyl-CoA


enzyme_display_name: thiamine phosphate phosphatase

substrate_name: thiamine phosphate

proof_lines: membrane-bound enzyme form, at pH 9.0, temperature not specified in the publication

proof_lines: soluble enzyme form, at pH 6.0, temperature not specified in the publication

enzyme: thiamine phosphate phosphatase

substrate: thiamine phosphate


organism: Zea mays

assay_conditions_summary: pH 5

proof_lines: at pH 5.0, temperature not specified in the publication


organism: Cajanus indicus

substrate_name: p-nitrophenyl beta-L-arabinoside

substrate: p-nitrophenyl beta-L-arabinoside


organism: Caldanaerobius polysaccharolyticus

substrate_name: 4-nitrophenyl beta-L-arabinopyranoside

substrate: 4-nitrophenyl beta-L-arabinopyranoside


enzyme_display_name: 2-phosphosulfolactate phosphatase

organism: Methanocaldococcus jannaschii

substrate_name: rac-phosphosulfolactate

enzyme: 2-phosphosulfolactate phosphatase

substrate: rac-phosphosulfolactate


enzyme_display_name: alanine carboxypeptidase

organism: Soil bacterium

substrate_name: N-benzoyl-L-aminobutyric acid

enzyme: alanine carboxypeptidase

substrate: N-benzoyl-L-aminobutyric acid


enzyme_display_name: dipeptidase E

enzyme: dipeptidase E


substrate_name: succinyl-casein

substrate: succinyl-casein


organism: Rattus norvegicus

substrate_name: Unknown

substrate: Unknown


enzyme_display_name: signal peptidase II

organism: Escherichia coli

substrate_name: prolipoprotein

enzyme: signal peptidase II

substrate: prolipoprotein


organism: ancestral Carnivora reconstruction


organism: Canis lupus familiaris

substrate_name: LSDGIDE

proof_lines: pH 7.2, 37°C, recombinant enzyme, calculated by BRENDA

substrate: LSDGIDE


proof_lines:
pH 7.5
pH 7.5, 37°C
at pH 7.0 and pH 7.5


enzyme_display_name: neprilysin

organism: Bos taurus

enzyme: neprilysin


proof_lines:
enzyme from glomeruli
enzyme from tubules


substrate_name: angiotensin II

proof_lines:
37°C, pH 7.4
unsulfated
sulfated

substrate: angiotensin II


organism: Lactococcus lactis

substrate_name: Met-enkephalin amide

substrate: Met-enkephalin amide


enzyme_display_name: thermolysin

organism: Bacillus sp. (in: firmicutes)

proof_lines: pH 7.0, 37°C, Co2+-bound enzyme, in presence of 1 M NaSCN

enzyme: thermolysin


organism: Bacillus thermoproteolyticus

substrate_name: N-carbobenzoxy-L-Asp-L-Phe methyl ester

proof_lines: pH 7.5, 25°C, recombinant wild-type enzyme

proof_lines: pH 7.0, 25°C, recombinant wild-type enzyme

substrate: N-carbobenzoxy-L-Asp-L-Phe methyl ester


substrate_name: GFSA

proof_lines:
pH 8.0, 37°C, 5 M glycerol, product GF
pH 7.5, 25°C, recombinant enzyme
pH 8.0, 37°C, product GF

substrate: GFSA


substrate_name: bradykinin

substrate: bradykinin


substrate_name: dansyl-Gly-Trp-Gly

substrate: dansyl-Gly-Trp-Gly


organism: Sus scrofa

substrate_name: Substance P

substrate: Substance P


enzyme_display_name: N(alpha)-acyl-L-glutamine aminoacylase

organism: Corynebacterium striatum

substrate_name: benzyloxycarbonyl-L-glutamine

enzyme: N(alpha)-acyl-L-glutamine aminoacylase

substrate: benzyloxycarbonyl-L-glutamine


enzyme_display_name: acylagmatine amidase

organism: Fusarium anguioides

substrate_name: propionyl agmatine

enzyme: acylagmatine amidase

substrate: propionyl agmatine


enzyme_display_name: amidinoaspartase

organism: Pseudomonas chlororaphis

substrate_name: N-amidino-L-aspartate

assay_conditions_summary: pH 8; buffer HEPES buffer

proof_lines:
HEPES buffer, pH 8, assay method B
phosphate buffer
HEPES buffer, pH 8, assay method A

enzyme: amidinoaspartase

substrate: N-amidino-L-aspartate


organism: Pseudomonas putida

proof_lines:
C-32 , activation with Co2+
C-32, activation with Mn2+
C-32


enzyme_display_name: guanidinoacetase

organism: Corynebacterium sp.

substrate_name: Guanidinoacetate

enzyme: guanidinoacetase

substrate: Guanidinoacetate


organism: Flavobacterium sp.


organism: Pseudomonas sp.


enzyme_display_name: salsolinol synthase

organism: Homo sapiens

substrate_name: acetaldehyde

proof_lines: with formaldehyde as cosubstrate, at pH 7.4 and 37°C

proof_lines: with acetaldehyde as cosubstrate, at pH 7.4 and 37°C

enzyme: salsolinol synthase

substrate: acetaldehyde


enzyme_display_name: dolichyldiphosphatase

substrate_name: dolichyl diphosphate

enzyme: dolichyldiphosphatase

substrate: dolichyl diphosphate


organism: Rattus norvegicus

proof_lines:
day 22 of embryonic development
day 4 of embryonic development


organism: Saccharomyces cerevisiae

proof_lines: pH 7.2, 30°C, using microsomal fractions from Sf-9 cells expressing the enzyme


enzyme_display_name: phenylacetaldehyde synthase

organism: Petunia x hybrida

substrate_name: L-phenylalanine

proof_lines: pH and temperature not specified in the publication

enzyme: phenylacetaldehyde synthase

substrate: L-phenylalanine


enzyme_display_name: trimethylamine-oxide aldolase

organism: Aminobacter aminovorans

substrate_name: Benzyldimethylamine N-oxide

enzyme: trimethylamine-oxide aldolase

substrate: Benzyldimethylamine N-oxide


substrate_name: Trimethylamine N-oxide

substrate: Trimethylamine N-oxide


organism: Dosidicus gigas

proof_lines: in 100 mM Tris-acetate (pH 7.0), 2 mM cysteine, 2 mM ascorbate, and 0.2 mM FeCl2, at 50°C


organism: Urophycis chuss

assay_conditions_summary: cofactor NADH

proof_lines: catalyzed aerobically in presence of Fe2+ (0.2 mM), ascorbate (2 mM), and cysteine (2 mM)

proof_lines: catalyzed anaerobically in presence of FMN (0.1 mM) and NADH (0.4 mM)


enzyme_display_name: 3-hydroxybutyryl-CoA dehydratase

substrate_name: Unknown

enzyme: 3-hydroxybutyryl-CoA dehydratase

substrate: Unknown


organism: Rhodospirillum rubrum

substrate_name: crotonyl-[acyl-carrier protein]

assay_conditions_summary: cofactor CoA

substrate: crotonyl-[acyl-carrier protein]


enzyme_display_name: arabinose-5-phosphate isomerase

organism: Acidipropionibacterium acidipropionici

substrate_name: D-arabinose 5-phosphate

enzyme: arabinose-5-phosphate isomerase

substrate: D-arabinose 5-phosphate


organism: Arabidopsis thaliana


organism: Escherichia coli


organism: Francisella tularensis

proof_lines: at 37°C, pH not specified in the publication


enzyme_display_name: cyanide hydratase

organism: Aspergillus niger

enzyme: cyanide hydratase


organism: Fusarium lateritium

substrate_name: CN-

substrate: CN-


organism: Stemphylium loti


enzyme_display_name: inositol 3-methyltransferase

organism: Vinca minor

substrate_name: S-Adenosyl-L-methionine; myo-Inositol

enzyme: inositol 3-methyltransferase

substrate: S-Adenosyl-L-methionine; myo-Inositol


organism: Hordeum vulgare

substrate_name: UTP; D-Glucuronate 1-phosphate

substrate: UTP; D-Glucuronate 1-phosphate


organism: Homo sapiens

substrate_name: H2O; Ac-Leu-Leu-Tyr-AFC

proof_lines: IC50 above 0.01 mM, pH and temperature not specified in the publication

substrate: H2O; Ac-Leu-Leu-Tyr-AFC


organism: Lactococcus lactis subsp. lactis


enzyme_display_name: formyltetrahydrofolate deformylase

organism: Sus scrofa

substrate_name: H2O; 10-Formyltetrahydrofolate

enzyme: formyltetrahydrofolate deformylase

substrate: H2O; 10-Formyltetrahydrofolate


enzyme_display_name: verruculogen prenyltransferase

organism: Aspergillus fischeri

substrate_name: fumitremorgin B

enzyme: verruculogen prenyltransferase

substrate: fumitremorgin B


organism: Petroselinum crispum

substrate_name: dimethylallyl diphosphate

proof_lines:
product osthenol, pH 8.0, 25°C
product demethylsuberosin, pH 8.0, 25°C

substrate: dimethylallyl diphosphate


organism: Methanothermobacter marburgensis

substrate_name: sn-Glyceryl phosphate; Geranylgeranyl diphosphate


organism: Methanothermobacter thermautotrophicus

substrate: sn-Glyceryl phosphate; Geranylgeranyl diphosphate


organism: Thermoplasma acidophilum


enzyme_display_name: macrolide 2'-kinase

substrate_name: ATP

enzyme: macrolide 2'-kinase

substrate: ATP


proof_lines:
pH 7.5, 37°C
Raw SKiD Env: Temp=37°C, pH=7.5


enzyme_display_name: keratan sulfotransferase

substrate_name: 3'-Phosphoadenylyl sulfate; Keratan sulfate

enzyme: keratan sulfotransferase