sequence_variant_note: Resolved canonical sequence from UniProtKB:P11980 direct fetch and applied mutation signature A398R with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant M1 A398R


enzyme_display_name: pyruvate kinase

organism: Saccharomyces cerevisiae

substrate_name: Phosphoenolpyruvate; ADP

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

protein_accession_database: UniProt

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

km_unit: mM

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant R336K

enzyme: pyruvate kinase

substrate: Phosphoenolpyruvate; ADP


substrate_name: ADP; Phosphoenolpyruvate

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant R336Q

substrate: ADP; Phosphoenolpyruvate


enzyme_display_name: beta-lactamase

organism: Chryseobacterium indologenes

substrate_name: Sulbactam

source_db: brenda

mutation_signature: Unknown

source_license: CC BY 4.0

sequence_source: UniProtKB REST direct lookup: Q6JE29

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

assay_conditions_summary: pH 6.5; buffer note

proof_lines: in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4

enzyme: beta-lactamase

substrate: Sulbactam

source: brenda


organism: Escherichia coli

substrate_name: cefotaxime; cefalotin

sequence_variant_note: Mutation signature R276G matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

substrate: cefotaxime; cefalotin


sequence_variant_note: Mutation signature R276N matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_variant_note: Mutation signature R276S matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


substrate_name: cefalotin; cefotaxime

sequence_variant_note: Mutation signature R276H matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

substrate: cefalotin; cefotaxime


substrate_name: cephalothin; penicillin G; cefotaxime; cefoxitin; ceftazidime

proof_lines: 30°C, pH 7.0. The affinity of MIR-4 beta-lactamase for ceftazidime is quite low


organism: Synechococcus elongatus

verification_status: unverified

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

status: unverified


sequence_source: UniProtKB P0AD61 canonical sequence from direct accession lookup.

assay_conditions_summary: cofactor ATP


organism: Homo sapiens

sequence_source: UniProtKB P30613 canonical sequence; reconstructed R510Q after direct residue check confirmed R at position 510.

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant R510Q


organism: Leishmania mexicana

substrate_name: ADP

sequence_source: UniProtKB direct accession lookup Q27686, retrieved 2026-05-29

assay_conditions_summary: pH 7.5; buffer is pH 7; cofactor ADP

substrate: ADP


organism: Trypanosoma brucei


organism: Aeromonas hydrophila

substrate_name: H2O; Imipenem

source_db: merged

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

sequence_source: UniProtKB:P26918 canonical sequence checked on 2026-05-29; variant sequence unresolved

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

assay_conditions_summary: pH 6.5; 303.15 K; buffer 20 mM sodium cacodylate

proof_lines: SABIO-RK modifier species: beta-lactamase(Enzyme) mutant N116H/N220G

proof_lines: mutant enzyme N116H/N220G, in 20 mM sodium cacodylate buffer, pH 6.5

substrate: H2O; Imipenem

source: merged


proof_lines: SABIO-RK modifier species: beta-lactamase(Enzyme) mutant N220G


organism: Bacillus cereus

substrate_name: Nitrocefin; H2O / Imipenem; H2O / related beta-lactam source rows

ki_unit: mM

proof_lines: SABIO-RK modifier species: beta-lactamase(Enzyme) mutant BcII delta IMP


proof_lines: SABIO-RK modifier species: beta-lactamase(Enzyme) mutant BcII delta IMP W64A

substrate: Nitrocefin; H2O / Imipenem; H2O / related beta-lactam source rows


substrate_name: Cefotaxime / Cephapirin / Imipenem / Nitrocefin source rows


substrate_name: Nitrocefin / Cefotaxime / Imipenem / Penicillin G source rows

proof_lines: SABIO-RK modifier species: beta-lactamase(Enzyme) mutant D120E; fusion protein to glutathione-S-transferase


proof_lines: SABIO-RK modifier species: beta-lactamase(Enzyme) mutant D120Q; fusion protein to glutathione-S-transferase

substrate: Nitrocefin / Cefotaxime / Imipenem / Penicillin G source rows


proof_lines: SABIO-RK modifier species: beta-lactamase(Enzyme) mutant D120N; fusion protein to glutathione-S-transferase


proof_lines: SABIO-RK modifier species: beta-lactamase(Enzyme) mutant D120S; fusion protein to glutathione-S-transferase


substrate_name: Nitrocefin / Cephalosporin C / 6-Aminopenicillanate / related source rows

proof_lines: SABIO-RK modifier species: beta-lactamase(Enzyme) mutant K73R


sequence_source: UniProtKB Q6JE29 canonical sequence checked 2026-05-29.

assay_conditions_summary: pH 6.5; buffer pH 6


organism: Spinacia oleracea

proof_lines:
isozyme PKII, pH 7.1, 25°C
isozyme PKI, pH 7.1, 25°C
pH 7.1, 25°C


organism: Brassica napus

assay_conditions_summary: pH 6.8; 297.15 K; cofactor ATP


organism: Entamoeba histolytica

substrate_name: phosphoenolpyruvate

assay_conditions_summary: 299.15 K; cofactor ADP

substrate: phosphoenolpyruvate


organism: Mucor rouxii

km_display: Not reported


organism: Plasmodium falciparum


organism: Synechococcus sp.

sequence_source: UniProtKB:A0A0H3K2W0 direct FASTA; organism_id 269084 Synechococcus sp. PCC 6301

proof_lines:
allosteric enzyme, pH 7.5, 24°C
allosteric enzyme, pH 7.0, 24°C


organism: Toxoplasma gondii

assay_conditions_summary: pH 8.5; 310.15 K; cofactor ATP

proof_lines: PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C


organism: Urocitellus richardsonii

assay_conditions_summary: pH 7; 298.15 K; cofactor ADP

proof_lines: purified enzyme, pH 7.0, 25°C/room temperature


organism: Bos taurus


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0AD61 REST lookup 2026-05-29; reconstructed variant K382Q from canonical sequence and applied mutation signature K382Q to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant K382Q


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0AD61 REST lookup 2026-05-29; reconstructed variant R431E from canonical sequence and applied mutation signature R431E to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant R431E


organism: Rattus norvegicus

sequence_source: UniProtKB direct accession lookup for B1WBN9; TrEMBL entry B1WBN9_RAT is Rattus norvegicus pyruvate kinase with EC 2.7.1.40.


substrate_name: Pyruvate; ATP

substrate: Pyruvate; ATP


organism: Ricinus communis

sequence_source: UniProtKB direct accession lookup for Q43117; reviewed Swiss-Prot entry KPYA_RICCO is Ricinus communis pyruvate kinase isozyme A, chloroplastic, EC 2.7.1.40.


organism: Vibrio cholerae serotype O1

sequence_source: UniProtKB:Q9KUN0 direct REST lookup, retrieved 2026-05-29

assay_conditions_summary: pH 7; 298.15 K; cofactor ATP

proof_lines: pH 7.0, 25°C, recombinant non-His-tagged enzyme

proof_lines: pH 7.0, 25°C, recombinant His-tagged enzyme, in presence of fructose 1,6-bisphosphate


sequence_source: UniProtKB:Q9KQJ0 direct REST lookup, retrieved 2026-05-29

proof_lines: pH 7.0, 25°C, recombinant His-tagged enzyme, in presence of glucose 6-phosphate


organism: Homo sapiens

sequence_source: UniProt P30613 reviewed canonical sequence fetched directly; R510Q applied after confirming residue 510 is R.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant R510Q


organism: Corynebacterium glutamicum

sequence_source: UniProtKB REST accession Q46078 (UniProtKB reviewed Swiss-Prot), checked 2026-05-29

assay_conditions_summary: pH 7; 308.15 K; cofactor ATP


organism: Oncorhynchus mykiss

substrate_name: AMP; Phosphate; Phosphoenolpyruvate

substrate: AMP; Phosphate; Phosphoenolpyruvate


organism: Oryctolagus cuniculus

sequence_source: uniprot:P11974; direct REST lookup 2026-05-29


sequence_source: UniProtKB:P11980 checked directly; row-specific A398R/E432D sequence not reconstructed because residue coordinates did not validate

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant M1 A398R/E432D


organism: Saccharomyces cerevisiae

sequence_source: UniProt P00549 FASTA checked 2026-05-29; reconstructed R409Q variant after canonical residue validation

sequence_variant_note: Mutation signature R409Q matches the mutant residue at offset +0 and the reference residue at offset +6; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant R409Q


organism: Trachemys scripta elegans

proof_lines:
anoxic muscle enzyme, pH 6.6, 22°C
muscle enzyme, pH 7.2, 22°C
muscle enzyme, pH 6.6, 22°C


proof_lines:
liver enzyme, pH 7.2, 22°C
anoxic liver enzyme, pH 7.2, 22°C
liver enzyme, pH 6.6, 22°C


organism: Trichomonas vaginalis


organism: Morganella morganii subsp. morganii

substrate_name: cephalothin

sequence_source: UniProtKB direct lookup Q704Y9; EMBL AJ620363 / protein CAF04714.1; strain PP37

assay_conditions_summary: pH 7; 303.15 K; buffer context

proof_lines: in 20 mM phosphate buffer, (pH 7.0) at 30°C

substrate: cephalothin


organism: Streptococcus sanguis


organism: Vigna radiata

assay_conditions_summary: pH 6.5; 303.15 K; cofactor ADP


organism: Trypanosoma brucei

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant L76R


proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant R22G


enzyme_display_name: Beta-enolase

source_db: oed

sequence_variant_note: Resolved canonical sequence from UniProtKB:P25704 REST FASTA retrieved 2026-05-30 and applied mutation signature G37A with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.

data_origin: experimental

enzyme: Beta-enolase

source: oed


kcat_over_km_origin: calculated

sequence_source: UniProtKB:P25704 REST FASTA retrieved 2026-05-30


sequence_variant_note: Resolved canonical sequence from UniProtKB:P25704 REST FASTA retrieved 2026-05-30 and applied mutation signature G41A with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: phosphopyruvate hydratase(Enzyme) mutant Enolase G41A


proof_lines: SABIO-RK modifier species: phosphopyruvate hydratase(Enzyme) mutant Enolase G37A


enzyme_display_name: phosphopyruvate hydratase

organism: Canis familiaris

enzyme: phosphopyruvate hydratase


organism: Chloroflexus aurantiacus

source_license: CC BY 4.0; CC BY-NC-ND 4.0

sequence_source: UniProt REST accession A9WCM4, direct lookup 2026-05-30

proof_lines:
pH 6.5, 80°C
pH 6.5, 25°C
Raw SKiD Env: Temp=80°C, pH=6.5

source_databases_merged: brenda skid


organism: Entamoeba histolytica

substrate_name: H2O; Phosphoenolpyruvate

sequence_source: UniProt REST accession P51555, direct lookup 2026-05-30

substrate: H2O; Phosphoenolpyruvate


organism: Escherichia coli

sequence_source: UniProt REST accession P0A6P9, direct lookup 2026-05-30


organism: Glycine max

sequence_source: UniProt REST accession Q6RIB7, direct lookup 2026-05-30


sequence_source: UniProt REST accession P13929, direct lookup 2026-05-30


sequence_source: UniProt REST accession P06733, direct lookup 2026-05-30


sequence_source: UniProt REST accession P09104, direct lookup 2026-05-30


organism: Leishmania mexicana

sequence_source: UniProtKB Q3HL75 exact accession lookup, fetched 2026-05-30

proof_lines: recombinant protein, substrate 2-phospho-D-glycerate


organism: Leuconostoc mesenteroides

sequence_source: UniProtKB Q1ET73 exact accession lookup, fetched 2026-05-30

proof_lines:
at pH 6.8
in absence of phosphate
in presence of 1 mM phosphate


organism: Mus musculus

sequence_source: UniProtKB P17182 exact accession lookup, fetched 2026-05-30


sequence_source: UniProtKB P17183 exact accession lookup, fetched 2026-05-30


sequence_source: UniProtKB P25704 exact accession lookup plus direct E414L reconstruction, fetched/computed 2026-05-30

proof_lines: SABIO-RK modifier species: phosphopyruvate hydratase(Enzyme) mutant E414L


substrate_name: 3-Fluoro 2-phospholactate

sequence_source: UniProtKB:P25704 direct REST lookup (https://rest.uniprot.org/uniprotkb/P25704.json)

substrate: 3-Fluoro 2-phospholactate


organism: Plasmodium falciparum

sequence_source: UniProtKB:Q8IJN7 direct REST lookup (https://rest.uniprot.org/uniprotkb/Q8IJN7.json)


sequence_source: UniProtKB:Q27727 direct REST lookup (https://rest.uniprot.org/uniprotkb/Q27727.json)

proof_lines:
acitivity for monomers
activity for dimeric form


organism: Pyrococcus furiosus


sequence_source: UniProtKB:Q8U477 direct REST lookup (https://rest.uniprot.org/uniprotkb/Q8U477.json)


organism: Rattus norvegicus

sequence_source: UniProtKB:P04764 direct REST lookup (https://rest.uniprot.org/uniprotkb/P04764.json)


organism: Ricinus communis

sequence_source: UniProtKB:P42896 direct REST lookup (https://rest.uniprot.org/uniprotkb/P42896.json)


organism: Anaplasma phagocytophilum

sequence_source: UniProtKB Q2GK24 direct REST lookup on 2026-05-30


organism: Aphidius ervi

sequence_source: UniProtKB C9YSH7 direct REST lookup on 2026-05-30

proof_lines: in 81 mM triethanolamine/HCl, pH 7.4, at 25°C


organism: Bacillus subtilis

sequence_source: UniProtKB P37869 direct REST lookup on 2026-05-30


organism: Bacteroides fragilis

sequence_source: UniProtKB Q8KNX9 direct REST lookup on 2026-05-30

assay_conditions_summary: 310.15 K; buffer reaction


organism: Bos taurus

sequence_source: UniProtKB Q3ZC09 direct REST lookup on 2026-05-30


sequence_source: UniProtKB Q9XSJ4 direct REST lookup on 2026-05-30


organism: Brucella abortus

sequence_source: UniProtKB Q2YPV0 direct REST lookup on 2026-05-30

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


organism: Oryctolagus cuniculus

sequence_variant_note: Resolved canonical sequence from UniProtKB:P25704 canonical sequence; G37A reconstructed using PMID 15752688 mature-protein numbering with UniProt offset +1 and applied mutation signature G37A with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:P25704 canonical sequence; G37A reconstructed using PMID 15752688 mature-protein numbering with UniProt offset +1


sequence_variant_note: Resolved canonical sequence from UniProtKB:P25704 canonical sequence; G41A reconstructed using PMID 15752688 mature-protein numbering with UniProt offset +1 and applied mutation signature G41A with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:P25704 canonical sequence


sequence_source: UniProtKB:P25704 canonical sequence; G41A reconstructed using PMID 15752688 mature-protein numbering with UniProt offset +1


organism: Saccharolobus solfataricus

sequence_source: UniProtKB:Q97ZJ3 FASTA fetched directly on 2026-05-30.

proof_lines: 65°C, pH not specified in the publication

proof_lines: 80°C, pH not specified in the publication

proof_lines: 70°C, pH not specified in the publication


organism: Saccharomyces cerevisiae

sequence_source: UniProtKB:P00924 FASTA fetched directly on 2026-05-30; S39A reconstructed by applying the paper residue numbering to UniProt residue 40.

proof_lines: SABIO-RK modifier species: phosphopyruvate hydratase(Enzyme) mutant S39A


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


sequence_source: UniProtKB:P00924 FASTA fetched directly on 2026-05-30.

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


sequence_source: UniProtKB:P00924 FASTA fetched directly on 2026-05-30; N80D/N126D reconstructed by applying the paper residue numbering to UniProt residues 81 and 127.

sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset +1, so it is treated as a source-provided variant sequence.


sequence_source: UniProtKB:P0CX11 FASTA fetched directly on 2026-05-30.


verification_status: verified

assay_conditions_summary: pH 6.5; 303.15 K; buffer a standardised reaction

column_label: Section 3.4, decoded plain-ASCII restatement, Eno1p with 3-phosphoglycerate: kcat (s^-1) = 5.07 (SEM 2.9%); Km (mM) = 0.617 (SEM 10.2%). The article prints no kcat/Km value for this measurement.

normalized_value_unit_evidence: kcat: printed value 5.07 s^-1 -> 5.07 s^(-1); the catalog holds 5.07 s^(-1); no unit conversion applied (the paper prints the reciprocal second directly, written s^-1 in the decoded block and s−1 in the article). Km: printed value 0.617 mM -> 0.617 mM; the catalog holds 0.617 mM; no unit conversion applied (the paper prints mM). kcat/Km: The catalog kcat_over_km 8.217179902755268 s^(-1)*mM^(-1) is NOT a printed cell: this paper prints no kcat/Km, catalytic-efficiency or specificity-constant value anywhere, in Table 2 or in its text. The catalog value is the exact full-precision quotient of this measurement's own printed kcat and Km, 5.07 s^-1 / 0.617 mM, so it is typed derived_same_measurement rather than quoted.

row_label: Section 3.4, the sentence reporting 3-phosphoglycerate as a substrate for Eno1p (decoded plain-ASCII restatement)

table_label: Table 2 'Enzyme kinetics parameters determined in this project and contrasted with those of [23]'

status: verified


sequence_source: UniProtKB:P00925 FASTA fetched directly on 2026-05-30.

assay_conditions_summary: pH 6.5; 303.15 K; buffer a standardised reaction; cofactor NADPH

column_label: Table label: Table 2, "Enzyme kinetics parameters determined in this project and contrasted with those of Ref. [23]". Reaction: ENO. Isoenzyme: Eno2p. Rows read: Parameter kcat, Value cell 19.9, Unit cell s−1; Parameter Kp2g, Value cell 0.104, Unit cell mM. metric: kcat; raw value: 19.9; raw unit: s^-1; normalized value: 19.9 s^(-1); metric: Km; raw value: 0.104; raw unit: mM; normalized value: 0.104 mM; metric: kcat/Km; raw unit: s^-1 mM^-1; normalized value: 191.34615384615384 s^-1 mM^-1; assay condition: Spectrophotometric assays of the glycolytic isoenzymes measuring consumption or production of NADH or NADPH; all assays were performed in a standardised reaction buffer at 30 °C in 384-well format plates with a 60 μl reaction volume, reagents in 45 μl, enzyme in 5 μl and substrate in 10 μl, with duplicate initial-rate measurements fitted to Michaelis-Menten kinetics. Export row conditions: pH 6.5; 303.15 K.

normalized_value_unit_evidence: Table 2 rows for ENO | Eno2p: kcat 19.9 s−1 = 19.9 s^(-1) (the paper's unit s−1 is already the export unit s^(-1), no conversion); Kp2g 0.104 mM = 0.104 mM (the paper's Km-equivalent cell is already in the export unit mM, no conversion); kcat/Km 19.9 s^-1 mM^-1 = 191.34615384615384 s^-1 mM^-1 divided as 19.9 / 0.104. In the on-disk transcription the superscript minus one renders as the flattened `s−1` (U+2212 minus sign) and empty SEM/Ref cells render as empty ` | ` fields, so the kcat row prints as `ENO | Eno2p | kcat | 19.9 |  |  | s−1`.

raw_value_unit_evidence: Table 2, "Enzyme kinetics parameters determined in this project and contrasted with those of Ref. [23]" | header row: Reaction | Isoenzyme | Parameter | Value | SEM (%) | Ref. [23] | Unit | kcat row: ENO | Eno2p | kcat | 19.9 |  |  | s−1 | Kp2g row: ENO | Eno2p | Kp2g | 0.104 |  | 0.04 | mM | cells read: kcat = 19.9 s−1 (Value column, Unit column s−1); Kp2g = 0.104 mM (Value column, Unit column mM)

row_label: ENO | Eno2p


proof_lines:
cytosolic isoenzyme
plastidic isoenzyme


sequence_source: UniProtKB:P00924 FASTA


sequence_source: UniProtKB:P00925 FASTA

assay_conditions_summary: 303.15 K; buffer a standardised reaction; cofactor NADPH

column_label: Table label: Table 2, "Enzyme kinetics parameters determined in this project and contrasted with those of Ref. [23]". Reaction: ENO. Isoenzyme: Eno2p. Rows read: ENO | Eno2p | kcat -> Value cell 19.9, Unit cell s−1; ENO | Eno2p | Kp2g -> Value cell 0.104, Unit cell mM. kcat/Km: no printed cell (Table 2 has no kcat/Km column).

normalized_value_unit_evidence: kcat 19.9 s−1 = 19.9 s^(-1) (printed unit s−1 is the export unit s^(-1), no conversion); decimal-equal to the catalog kcat 19.9; Km (Kp2g) 0.104 mM = 0.104 mM (already the export unit, no conversion); decimal-equal to the catalog km 0.104. In the on-disk transcription the printed superscript minus renders as the flattened `s−1` (U+2212) and empty SEM/Ref cells render as empty ` | ` fields.

raw_value_unit_evidence: Table 2, "Enzyme kinetics parameters determined in this project and contrasted with those of Ref. [23]" | header row: Reaction | Isoenzyme | Parameter | Value | SEM (%) | Ref. [23] | Unit | kcat evidence: ENO | Eno2p | kcat | 19.9 |  |  | s−1 | Km evidence: ENO | Eno2p | Kp2g | 0.104 |  | 0.04 | mM | cells read: kcat = 19.9 s−1 (Value column); Kp2g = 0.104 mM


sequence_source: UniProtKB:P42222 FASTA


source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0


organism: Spinacia oleracea

sequence_source: UniProtKB Q9LEE0 direct lookup on 2026-05-30; entry Q9LEE0_SPIOL, unreviewed, phosphopyruvate hydratase, organism Spinacia oleracea, EC 4.2.1.11.


organism: Streptococcus iniae

sequence_source: UniProtKB T1TFA0 direct lookup on 2026-05-30; entry T1TFA0_STRIN, unreviewed, enolase, organism Streptococcus iniae, EC 4.2.1.11.

assay_conditions_summary: pH 7

proof_lines: pH 7.0, temperature not specified in the publication


organism: Streptococcus mutans

sequence_source: UniProtKB Q8DTS9 direct lookup on 2026-05-30; entry ENO_STRMU, reviewed, enolase, organism Streptococcus mutans serotype c strain ATCC 700610 / UA159, EC 4.2.1.11.


organism: Streptococcus pneumoniae

sequence_source: UniProtKB Q97QS2 direct lookup on 2026-05-30; entry ENO_STRPN, reviewed, enolase, organism Streptococcus pneumoniae serotype 4 strain ATCC BAA-334 / TIGR4, EC 4.2.1.11.


organism: Streptococcus pyogenes

sequence_source: UniProtKB Q5XD01 direct lookup on 2026-05-30; entry ENO_STRP6, reviewed, enolase, organism Streptococcus pyogenes serotype M6 strain ATCC BAA-946 / MGAS10394, EC 4.2.1.11.


organism: Streptococcus sanguis

sequence_source: UniProtKB A0A024F8D9 direct lookup on 2026-05-30; entry A0A024F8D9_STRSA, unreviewed, enolase, organism Streptococcus sanguinis, EC 4.2.1.11.


organism: Streptococcus suis

sequence_source: UniProtKB C6GGT7 direct lookup on 2026-05-30; entry C6GGT7_STRSU, unreviewed, enolase, organism Streptococcus suis, EC 4.2.1.11.

proof_lines: in 100 mM HEPES, 10 mM MgCl2, and 7.7 mM KCl, pH 7.0, at 37°C


organism: Sus scrofa

sequence_source: UniProtKB Q1KYT0 direct lookup on 2026-05-30; entry ENOB_PIG, reviewed, beta-enolase, organism Sus scrofa, EC 4.2.1.11.


organism: Trypanosoma brucei

sequence_source: UniProtKB REST accession lookup for Q9NDH8 on 2026-05-30

proof_lines: at pH 7.2, binds in the same way as phosphoenolpyruvate and phosphonoacetohydroxamate


sequence_source: UniProtKB REST accession lookup for Q38BV6 on 2026-05-30


organism: Ustilago maydis

sequence_source: UniProtKB REST accession lookup for A0A0D1E2E4 on 2026-05-30


organism: Vibrio parahaemolyticus

sequence_source: UniProtKB REST accession lookup for W6ABG9 on 2026-05-30

assay_conditions_summary: pH 8.5; 298.15 K; buffer conditions

proof_lines: in 100 mM HEPES buffer, pH 8.5, 7.7 mM KCl, 10 mM MgSO4, at 25°C


organism: Xenopus laevis

sequence_source: UniProtKB REST accession lookup for P08734 on 2026-05-30


organism: Zymomonas mobilis

sequence_source: UniProtKB REST accession lookup for P33675 on 2026-05-30


organism: Arabidopsis thaliana

substrate_name: 2-PGA (at pH 8)

source_db: uniprot

assay_conditions_summary: pH 8

substrate: 2-PGA (at pH 8)

source: uniprot


organism: Bacillus subtilis (strain 168)


organism: Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)

substrate_name: 2-phosphoglycerate (2-PG) at 80 degrees Celsius


organism: Escherichia coli (strain K12)


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: phosphoenolpyruvate (expression in E.coli)


organism: Nitratidesulfovibrio vulgaris (strain DSM 19637 / Miyazaki F)

substrate_name: 2-phosphoglycerate (at 25 degrees Celsius and pH 8)


organism: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

substrate_name: 2-phospho-D-glycerate (at 75 degrees Celsius)


organism: Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)


organism: Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)


organism: Busycon canaliculatum


organism: Candida albicans

proof_lines: pH 6.8, 30ºC, in the absence of phosphate


organism: Cyprinus carpio


organism: Gallus gallus

proof_lines: brain enolase I, brain enolase II, brain enolase III


organism: Homo sapiens

assay_conditions_summary: pH 6.8; buffer 50 mM imidazole-HCl

proof_lines: in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl

proof_lines: native enzyme, in the absence of methyglyoxal, in 50 mM imidazole-HCl buffer, pH 6.8, with 0.4 M KCl and 3 mM MgSO4


organism: Ilyanassa obsoleta


organism: Actinomyces naeslundii


proof_lines:
at pH 8.0
at pH 7.0


organism: Archaeoglobus fulgidus

proof_lines: 50°C, pH not specified in the publication


organism: Bacillus anthracis


organism: Bacillus megaterium


organism: Bacteroides fragilis

proof_lines: pH and temperature not specified in the publication


organism: Klebsiella pneumoniae

assay_conditions_summary: pH 7.8; buffer 50 mM imidazole-HCl

proof_lines: at low phosphate concentrations (4-6 mM), in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl


organism: Lactobacillus rhamnosus


organism: Littorina littorea


organism: Macaca mulatta


organism: Mercenaria mercenaria


organism: Mus musculus


organism: Mytilus edulis


organism: Nucella lapillus


organism: Oncorhynchus keta


organism: Oncorhynchus kisutch


organism: Oryctolagus cuniculus


organism: Pagrus major


organism: Planorbis corneus


organism: Plasmodium falciparum

proof_lines: recombinant, His-tagged, hexameric enzyme


organism: Priestia megaterium


organism: Rattus norvegicus

substrate_name: Phosphoenolpyruvate; H2O

proof_lines:
liver enzyme
muscle enzyme
enolase III

substrate: Phosphoenolpyruvate; H2O


organism: Saccharomyces cerevisiae

proof_lines: isoform Eno1p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication

proof_lines: isoform Err2p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication


proof_lines:
wild-type enzyme
wild type enzyme


proof_lines: in presence of 1 mM Mg2+, mutant enzyme S39A


organism: Scomber japonicus


organism: Spinacia oleracea


organism: Streptococcus mutans


organism: Streptococcus ratti


organism: Streptococcus salivarius


organism: Streptococcus sanguis


organism: Sus scrofa

proof_lines:
pH 6.8, liver enolase
pH 7.4, muscle enolase
pH 6.8, muscle enolase


organism: Teladorsagia circumcincta


organism: Thermotoga maritima

sequence_source: UniProtKB:P42848 reviewed Swiss-Prot ENO_THEMA; EC 4.2.1.11 evidence includes PMID:7757011


organism: Taenia pisiformis

sequence_source: UniProtKB REST exact accession lookup: T1WGD6

proof_lines: 22°C, pH not specified in the publication


organism: Taenia solium

sequence_source: UniProtKB REST exact accession lookup: V5T827

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Theileria annulata

sequence_source: UniProtKB REST exact accession lookup: E9NJD0


proof_lines: 1.5 mM MgCl2 in 50 mM Tris/HCl (pH 7.4), at 25°C


organism: Toxoplasma gondii

sequence_source: UniProtKB REST exact accession lookup: Q9BPL7


sequence_source: UniProtKB REST exact accession lookup: Q9UAE6


proof_lines: SABIO-RK modifier species: phosphopyruvate hydratase(Enzyme) mutant ENO1deltaEWGYS

proof_lines: SABIO-RK modifier species: phosphopyruvate hydratase(Enzyme) mutant ENO1deltaEKdeltaEWGYS


organism: Trachemys scripta

sequence_source: UniProtKB REST exact accession lookup: Q9W7L1


organism: Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM)

substrate_name: 2-phosphoglycerate (at pH 7 and 37 degrees Celsius)

proof_lines: kcat is 170 sec(-1) with 2-phosphoglycerate as substrate (at pH 7 and 37 degrees Celsius) (PubMed:15794763). kcat is 147 sec(-1) with 2-phosphoglycerate as substrate (at pH 6 and 37 degrees Celsius) (PubMed:15794763). kcat is 43 sec(-1) wit


organism: Plasmodium falciparum (isolate 3D7)

substrate_name: 2-phosphoglyceric acid (at 21 degrees Celsius and pH 7.5)

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

proof_lines: kcat is 20.6 sec(-1) with 2-phosphoglyceric acid as substrate (at 21 degrees Celsius and pH 7.5).


organism: Staphylococcus aureus

substrate_name: 2-phosphoglycerate by octamer

substrate: 2-phosphoglycerate by octamer


organism: Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)

proof_lines: Also catalytically active when expressed on the bacterial cell surface.


organism: Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)

proof_lines: Catalytically active also when expressed on the bacterial cell surface.


organism: Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805)


organism: Amycolatopsis methanolica

substrate_name: Phosphoenolpyruvate; ADP

sequence_source: UniProtKB A0A076MW80 REST lookup, checked 2026-05-30

assay_conditions_summary: cofactor ATP

substrate: Phosphoenolpyruvate; ADP


organism: Bos taurus


organism: Chlamydia trachomatis


organism: Corynebacterium glutamicum

substrate_name: ADP; Phosphoenolpyruvate

substrate: ADP; Phosphoenolpyruvate


organism: Escherichia coli

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant K382Q


proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant R431E


sequence_source: UniProtKB:P0AD61 canonical sequence checked on 2026-05-30; selected payload has no mutation signature.


organism: Homo sapiens

sequence_source: UniProt P30613 direct accession lookup


organism: Lactococcus lactis subsp. cremoris

sequence_source: UniProt A2RKA3 direct accession lookup


organism: Lactococcus lactis subsp. lactis

sequence_source: UniProt Q07637 direct accession lookup


organism: Saccharomyces cerevisiae

sequence_source: UniProt P00549 canonical sequence retrieved 2026-05-30; Y436F applied after confirming residue 436 is Y.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant Y436F


sequence_source: UniProt P00549 canonical sequence retrieved 2026-05-30; Q299N applied after confirming residue 299 is Q.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant Q299N


sequence_source: UniProt P00549 canonical sequence retrieved 2026-05-30; A458K applied after confirming residue 458 is A.

sequence_variant_note: Mutation signature A458K matches the mutant residue at offset +0 and the reference residue at offset +9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant A458K


sequence_source: UniProt P00549 canonical sequence retrieved 2026-05-30; K236Q applied after confirming residue 236 is K.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant K236Q


sequence_source: UniProt P00549 canonical sequence retrieved 2026-05-30; Y436S applied after confirming residue 436 is Y.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant Y436S


sequence_source: UniProt P00549 canonical sequence retrieved 2026-05-30; T406R applied after confirming residue 406 is T.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant T406R


sequence_source: UniProt P00549 canonical sequence retrieved 2026-05-30; R409Q applied after confirming residue 409 is R.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant R409Q


sequence_source: UniProtKB P00549 canonical sequence checked directly; reconstructed by applying R369A after residue validation.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant R369A


sequence_source: UniProtKB P00549 canonical sequence checked directly; reconstructed by applying T403K after residue validation.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant T403K


sequence_source: UniProtKB P00549 canonical sequence checked directly; reconstructed by applying R459Q after residue validation.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant R459Q


sequence_source: UniProtKB P00549 canonical sequence checked directly; reconstructed by applying K292Q after residue validation.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant K292Q


sequence_source: UniProtKB P00549 canonical sequence checked directly; reconstructed by applying T311M after residue validation.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant T311M


sequence_source: UniProtKB P00549 canonical sequence checked directly; reconstructed by applying E392A after residue validation.

sequence_variant_note: Mutation signature E392A matches the mutant residue at offset +0 and the reference residue at offset -12; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant E392A


sequence_source: UniProtKB P00549 canonical sequence checked directly; reconstructed by applying R77Q after residue validation.

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant R77Q


sequence_source: UniProtKB P00549 canonical sequence checked directly; reconstructed by applying T298S after residue validation.

assay_conditions_summary: pH 6.8; cofactor ATP

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant isoenzyme Pyk1 T298S


organism: Selenomonas ruminantium

substrate_name: phosphoenolpyruvate

sequence_source: UniProtKB Q9LBS6 direct lookup


organism: Thermofilum pendens

substrate_name: ADP

sequence_source: UniProtKB A1RX09 direct lookup

proof_lines: pH 6.0, 45°C, recombinant enzyme with His6 tag


sequence_source: UniProtKB REST accession check for P00549 on 2026-05-30 with T403E substitution applied after confirming canonical residue T at position 403

proof_lines: SABIO-RK modifier species: pyruvate kinase(Enzyme) mutant T403E


organism: Trichomonas vaginalis

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


organism: Ustilago maydis

sequence_source: UniProtKB A0A0D1E735 REST accession lookup on 2026-05-30


organism: Zymomonas mobilis

sequence_source: UniProtKB Q5NR78 REST accession lookup on 2026-05-30


organism: Chryseobacterium indologenes

substrate_name: Sulbactam

sequence_source: UniProtKB Q6JE29 direct REST check on 2026-05-30

assay_conditions_summary: pH 6.5; buffer the selected row: 30 mM sodium cacodylate

proof_lines: in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4

substrate: Sulbactam


organism: Rattus norvegicus

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ADP

proof_lines:
apparent value
pH 7.1, 37°C
R-type isozyme, pH 7.4, 37°C


organism: Psychrobacter immobilis

substrate_name: ampicillin

sequence_source: UniProtKB:O05465 reviewed Psychrobacter immobilis ampC, PMID 9063463

assay_conditions_summary: pH 7; 303.15 K; buffer condition note 30 C in sodium phosphate

proof_lines: at 30°C in 50mM sodium phosphate buffer (pH 7.0)

substrate: ampicillin


enzyme_display_name: laccase

organism: Aeromonas hydrophila

substrate_name: 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid); O2 / 2,6-Dimethoxyphenol; O2

sequence_source: UniProtKB:D5FL31 REST lookup on 2026-05-30

enzyme: laccase


organism: alpha proteobacterium Q-1

substrate_name: 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid); O2 / I-; H+; O2


organism: Acidobacteriaceae


organism: Bacillus amyloliquefaciens

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant Lac3A/S, chimeric LacA (domain1 and 2)/LacS (domain 3) from Bacillus subtilis LS02


organism: Amycolatopsis sp.

sequence_source: UniProtKB:J9PBQ8 direct accession lookup on 2026-05-30


organism: Aquifex aeolicus

mutation_type: deletion

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant deltaP321-V363


organism: Bacillus licheniformis

sequence_source: UniProtKB:A0A0A6ZHX8 TrEMBL sequenceVersion 1, retrieved 2026-05-30


sequence_source: Reconstructed from UniProtKB:H8WGE4 TrEMBL sequenceVersion 1 by applying D500G; residue 500 was D in direct lookup, retrieved 2026-05-30

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant D500G


sequence_source: UniProtKB Q65MU7 REST lookup checked 2026-05-30; active TrEMBL laccase entry for Bacillus licheniformis strain ATCC 14580 / DSM 13 with EC 1.10.3.2 and 513-aa sequence. Variant sequence was reconstructed from Q65MU7 after residue checks.

assay_conditions_summary: pH 4; buffer 0.1 M citrate/phosphate


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant K316N/D500G


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant K316N


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant D501G


organism: Bacillus pumilus


sequence_source: UniProtKB A0A0G2QNK1 canonical sequence fetched by direct REST lookup on 2026-05-30; row-specific substitutions G417L applied after old-residue validation.

sequence_variant_note: Mutation signature G417L matches the mutant residue at offset +0 and the reference residue at offset -13; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant G417L N-terminal His6-tag


sequence_source: UniProtKB A0A0G2QNK1 canonical sequence fetched by direct REST lookup on 2026-05-30; row-specific substitutions L386W/G417L/D152W applied after old-residue validation.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant L386W/G417L/D152W N-terminal His6-tag


sequence_source: UniProtKB A0A0G2QNK1 canonical sequence fetched by direct REST lookup on 2026-05-30; row-specific substitutions L386W/G417L/G57L applied after old-residue validation.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant L386W/G417L/G57L N-terminal His6-tag


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant L386W/G417L/D152Y N-terminal His6-tag


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant L386W N-terminal His6-tag


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant L386W/G417L/D195W N-terminal His6-tag


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant L386W/G417L/D195F N-terminal His6-tag


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant L386W/G417L/D152F N-terminal His6-tag


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant L386W/G417L N-terminal His6-tag


sequence_source: UniProtKB:A0A0G2QNK1 canonical sequence checked 2026-05-30; L386W/G417L/G57W substitutions applied after confirming L386, G417, and G57 in the canonical sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant L386W/G417L/G57W N-terminal His6-tag


sequence_source: UniProtKB A0A0G2QNK1 canonical sequence with row mutation signature reconstructed during review

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant L386W/G417L/G57F N-terminal His6-tag


sequence_variant_note: Mutation signature F227A matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant F227A N-terminal His6-tag


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant S208G N-terminal His6-tag


sequence_variant_note: Mutation signature S208G/F227A matches the mutant residue at offset +0 and the reference residue at offset -29; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant S208G/F227A N-terminal His6-tag


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant T415D N-terminal His6-tag


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant T415D/Q442A N-terminal His6-tag


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant T418K/Q442A N-terminal His6-tag


sequence_source: UniProtKB:A0A0G2QNK1 canonical sequence with K317N/D501G/L386W/G417L/G57F reconstructed after residue-position checks

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant K317N/D501G/L386W/G417L/G57F


sequence_source: UniProtKB:A0A0G2QNK1 canonical sequence with D501G/L386W/G417L/G57F reconstructed after residue-position checks

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant D501G/L386W/G417L/G57F


sequence_source: UniProtKB:A0A0G2QNK1 canonical sequence with K317N/L386W/G417L/G57F reconstructed after residue-position checks

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant K317N/L386W/G417L/G57F


sequence_source: UniProtKB:A0A0G2QNK1 canonical sequence from direct accession lookup


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0G2QNK1; variant reconstruction applied from selected mutation_signature when present and applied mutation signature Q442A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant Q442A N-terminal His6-tag


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0G2QNK1; variant reconstruction applied from selected mutation_signature when present and applied mutation signature T418K to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant T418K N-terminal His6-tag


organism: Bacillus sp.

substrate_name: 2,6-Dimethoxyphenol; O2

sequence_source: UniProtKB exact accession lookup for B9W2C5 on 2026-05-30; active TrEMBL entry for Bacillus sp. HR03 laccase, EC 1.10.3.2, sequenceVersion 1.

substrate: 2,6-Dimethoxyphenol; O2


sequence_source: Unresolved: direct UniProtKB lookup on 2026-05-30 returned A0A222C745 as inactive/deleted with only UniParc UPI0003B0D0D2 exposed.


organism: Bacillus sp. HR03

substrate_name: O2; Syringaldazine

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant D500S

substrate: O2; Syringaldazine


substrate_name: Syringaldazine; O2

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant D500E

substrate: Syringaldazine; O2


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant inserted glycine at position 500


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant D500G


sequence_source: reconstructed from UniProtKB B9W2C5 canonical sequence by applying T415I

sequence_variant_note: Mutation signature T415I matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant T415I


sequence_source: reconstructed from UniProtKB B9W2C5 canonical sequence by applying T418I

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant T418I


sequence_source: reconstructed from UniProtKB B9W2C5 canonical sequence by applying E188V

sequence_variant_note: Mutation signature E188V matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E188V


sequence_source: reconstructed from UniProtKB B9W2C5 canonical sequence by applying E188I

sequence_variant_note: Mutation signature E188I matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E188I


sequence_source: reconstructed from UniProtKB B9W2C5 canonical sequence by applying E188L

sequence_variant_note: Mutation signature E188L matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E188L


sequence_source: UniProtKB B9W2C5 sequence version 1 fetched via UniProt REST on 2026-05-30; E188Y applied after confirming residue 188 is Glu.

sequence_variant_note: Mutation signature E188Y matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E188Y


sequence_source: UniProtKB B9W2C5 sequence version 1 fetched via UniProt REST on 2026-05-30; E188F applied after confirming residue 188 is Glu.

sequence_variant_note: Mutation signature E188F matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E188F


sequence_source: UniProtKB B9W2C5 sequence version 1 fetched via UniProt REST on 2026-05-30; selected record has no mutation signature, so canonical sequence is preserved as identity context.

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

unit: lgwedgeoneswedgeone


sequence_source: Reconstructed from UniProtKB B9W2C5 canonical sequence retrieved via UniProt REST on 2026-05-30 plus PMID 29425870 mutation signature.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant T415G/T418I


sequence_variant_note: Mutation signature T415G matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant T415G


organism: Bacillus subtilis

sequence_source: Reconstructed from UniProtKB P07788 canonical FASTA after confirming residue 502 is M and applying M502F; PMID 16680453 supports Met502 replacement by phenylalanine.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M502F


sequence_source: UniProtKB P07788 FASTA retrieved 2026-05-30; M502L reconstructed by substituting residue 502 M->L after confirming the canonical residue.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M502L


sequence_source: UniProtKB P07788 FASTA retrieved 2026-05-30; L386A reconstructed by substituting residue 386 L->A after confirming the canonical residue.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant L386A


sequence_variant_note: Resolved canonical sequence from UniProtKB P07788 canonical FASTA/JSON fetched 2026-05-30; Residue check: P07788 has Ile at position 494; I494A was reconstructed by substituting Ala at that position. and applied mutation signature I494A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant I494A


sequence_source: UniProtKB P07788 canonical sequence with G417L reconstructed during review after confirming residue 417 is G.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant G417L CotA-ABTS-1


sequence_source: UniProtKB P07788 canonical sequence with E498D reconstructed during review after confirming residue 498 is E.

sequence_variant_note: Mutation signature E498D matches the mutant residue at offset +0 and the reference residue at offset -2; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E498D


sequence_source: UniProtKB P07788 canonical sequence with E498T reconstructed during review after confirming residue 498 is E.

sequence_variant_note: Mutation signature E498T matches the mutant residue at offset +0 and the reference residue at offset -2; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E498T


sequence_source: UniProtKB P07788 canonical sequence with E498L reconstructed during review after confirming residue 498 is E.

sequence_variant_note: Mutation signature E498L matches the mutant residue at offset +0 and the reference residue at offset -2; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E498L


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant CotA-ABTS-SD1


sequence_source: UniProtKB:P07788 canonical CotA-laccase sequence, residue check C322, reconstructed by applying C322A.

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant C322A


sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B9W2C5 REST sequence checked 2026-05-30; reconstructed E188R from PMID 22112616 and SABIO-RK modifier note and applied mutation signature E188R to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E188R


sequence_variant_note: Resolved canonical sequence from UniProtKB:B9W2C5 REST sequence checked 2026-05-30; reconstructed E188K from PMID 22112616 and SABIO-RK modifier note and applied mutation signature E188K to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E188K


sequence_variant_note: Resolved canonical sequence from UniProtKB:B9W2C5 REST sequence checked 2026-05-30; reconstructed E188A from PMID 22112616 and SABIO-RK modifier note and applied mutation signature E188A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E188A


sequence_source: Reconstructed from UniProtKB P07788 canonical sequence by applying source mutation T415A after confirming residue 415 is T on 2026-05-30

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant T415A


sequence_source: UniProtKB:P07788 canonical sequence; reconstructed by applying E498G after direct residue checks on 2026-05-30.

sequence_variant_note: Mutation signature E498G matches the mutant residue at offset +0 and the reference residue at offset -2; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E498G


sequence_source: UniProtKB:P07788 canonical sequence; reconstructed by applying E29V after direct residue checks on 2026-05-30.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E29V


sequence_source: UniProtKB:P07788 canonical sequence; reconstructed by applying T480A/E498G after direct residue checks on 2026-05-30.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant T480A/E498G


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant F2071Y/V403T/P455S C-terminal His6-tagged


sequence_source: UniProtKB:P07788 canonical sequence; reconstructed by applying V403T/P455S after direct residue checks on 2026-05-30.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant V403T/P455S C-terminal His6-tagged


sequence_source: UniProtKB:P07788 canonical sequence; reconstructed by applying P455S after direct residue checks on 2026-05-30.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant P455S C-terminal His6-tagged


substrate_name: Syringaldazine

verification_status: corrected

sequence_variant_note: Resolved canonical sequence from UniProtKB P07788 canonical CotA sequence with L431F reconstructed after residue-coordinate check; PMID 27050268 Table 4 contains the L431F row. and applied mutation signature L431F to reconstruct the assayed variant sequence.

substrate: Syringaldazine

status: corrected


sequence_variant_note: Resolved canonical sequence from UniProtKB P07788 canonical CotA sequence with R146K reconstructed after residue-coordinate check; PMID 27050268 Table 4 contains the R146K row. and applied mutation signature R146K to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB P07788 canonical CotA sequence with R476K reconstructed after residue-coordinate check; PMID 27050268 Table 4 contains the R476K row. and applied mutation signature R476K to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB P07788 canonical CotA sequence with R429K reconstructed after residue-coordinate check; PMID 27050268 Table 4 contains R429K rows for both ABTS and SGZ. and applied mutation signature R429K to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant R429K


sequence_variant_note: Resolved canonical sequence from UniProtKB P07788 canonical CotA sequence with T480A reconstructed after residue-coordinate check; PMID 27050268 Table 4 contains the T480A row. and applied mutation signature T480A to reconstruct the assayed variant sequence.


sequence_source: UniProtKB P07788 canonical CotA sequence with L431F reconstructed after residue-coordinate check; PMID 27050268 Table 4 contains the L431F row.


sequence_variant_note: Resolved canonical sequence from UniProtKB P07788 canonical CotA sequence with R429K reconstructed after residue-coordinate check; PMID 27050268 Table 4 contains the R429K row. and applied mutation signature R429K to reconstruct the assayed variant sequence.


organism: Bacillus sp. LS03

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


sequence_variant_note: Resolved canonical sequence from UniProtKB:H8WGE3 reconstructed T232P/Q367R variant and applied mutation signature T232P/Q367R to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant 5E29 (T232P/Q367R), His-tag


sequence_source: UniProtKB:H8WGE3 reconstructed T232P/Q367R variant


organism: Bacillus sp. SL-1


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant R155W/M157H (CAWH)


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant R155W (CAW)


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant R155H (CAH2)


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A386JCD3 and applied mutation signature R155W/M157H/A158E to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant R155W/M157H/A158E (CAWH1)


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A1W6S310 and applied mutation signature T158A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant T158A (CAHH)


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A386JCD3 and applied mutation signature R155H/A158E to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant R155H/A158E (CAHE2)


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P07788 and applied mutation signature R429K to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 4; 313.15 K; buffer 100 mM citrate-phosphate

column_label: Table 4. Kinetic constants of CotA and its mutants for ABTS and SGZ, printed column heads Enzyme | ABTS: Km (uM), kcat (s^-1), kcat/Km (uM^-1 s^-1) | SGZ: Km (uM), kcat (s^-1), kcat/Km (uM^-1 s^-1) | (kcat/Km)ABTS/(kcat/Km)SGZ. Journal page 333 (PDF page 6). Enzyme row R429K; ABTS column group; Km cell = 90.61 uM; kcat cell = 1.71 s^-1; kcat/Km cell = 0.02 uM^-1 s^-1. captured primary segment: Table 4, ABTS column group, enzyme row R429K: Km 90.61 uM; kcat 1.71 s^-1; kcat/Km 0.02 uM^-1 s^-1.

normalized_value_unit_evidence: Km: printed Table 4 cell 90.61 uM; 1 uM = 1/1000 mM, so 90.61 / 1000 = 0.09061 mM; the export stores 0.09061 mM, the same value. kcat: printed Table 4 cell 1.71 s^-1; the printed turnover column is already per second, so the cell is carried across unchanged as 1.71 s^-1; the export stores 1.71 s^-1, the same value. kcat/Km: printed Table 4 cell 0.02 under the column head kcat/Km (uM^-1 s^-1); 1 uM^-1 = 1000 mM^-1, so 1 uM^-1 s^-1 = 1000 s^-1 mM^-1 and 0.02 x 1000 = 20.0 s^-1 mM^-1; the export stores 0.02 s^-1 mM^-1, which is the printed per-micromolar cell carrying the per-millimolar label without being multiplied by 1000.

raw_value_unit_evidence: Journal page 333, Table 4, enzyme row R429K, ABTS column group: Km = 90.61 uM; kcat = 1.71 s^-1; kcat/Km = 0.02 under the printed column head kcat/Km (uM^-1 s^-1).

row_label: Table 4, enzyme row R429K


sequence_variant_note: Resolved canonical sequence from UniProtKB:P07788 canonical CotA sequence, row-specific mutation reconstructed from selected mutation_signature and applied mutation signature L431F to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant L431F


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P07788 and applied mutation signature R476K to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 4; 313.15 K; buffer ethanol in 100 mM citrate-phosphate

column_label: Table 4. Kinetic constants of CotA and its mutants for ABTS and SGZ, printed column heads Enzyme | ABTS: Km (uM), kcat (s^-1), kcat/Km (uM^-1 s^-1) | SGZ: Km (uM), kcat (s^-1), kcat/Km (uM^-1 s^-1) | (kcat/Km)ABTS/(kcat/Km)SGZ. Journal page 333 (PDF page 6). Enzyme row R476K; SGZ column group; Km cell = 29.32 uM; kcat cell = 4.27 s^-1; kcat/Km cell = 0.15 uM^-1 s^-1. captured primary segment: Table 4, SGZ column group, enzyme row R476K: Km 29.32 uM; kcat 4.27 s^-1; kcat/Km 0.15 uM^-1 s^-1.

normalized_value_unit_evidence: Km: printed Table 4 cell 29.32 uM; 1 uM = 1/1000 mM, so 29.32 / 1000 = 0.02932 mM; the export stores 0.02932 mM, the same value. kcat: printed Table 4 cell 4.27 s^-1; the printed turnover column is already per second, so the cell is carried across unchanged as 4.27 s^-1; the export stores 4.27 s^-1, the same value. kcat/Km: printed Table 4 cell 0.15 under the column head kcat/Km (uM^-1 s^-1); 1 uM^-1 = 1000 mM^-1, so 1 uM^-1 s^-1 = 1000 s^-1 mM^-1 and 0.15 x 1000 = 150.0 s^-1 mM^-1; the export stores 0.15 s^-1 mM^-1, which is the printed per-micromolar cell carrying the per-millimolar label without being multiplied by 1000.

raw_value_unit_evidence: Journal page 333, Table 4, enzyme row R476K, SGZ column group: Km = 29.32 uM; kcat = 4.27 s^-1; kcat/Km = 0.15 under the printed column head kcat/Km (uM^-1 s^-1).

row_label: Table 4, enzyme row R476K


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P07788 and applied mutation signature R146K to reconstruct the assayed variant sequence.

column_label: Table 4. Kinetic constants of CotA and its mutants for ABTS and SGZ, printed column heads Enzyme | ABTS: Km (uM), kcat (s^-1), kcat/Km (uM^-1 s^-1) | SGZ: Km (uM), kcat (s^-1), kcat/Km (uM^-1 s^-1) | (kcat/Km)ABTS/(kcat/Km)SGZ. Journal page 333 (PDF page 6). Enzyme row R146K; SGZ column group; Km cell = 13.45 uM; kcat cell = 0.05 s^-1; kcat/Km cell = 0.004 uM^-1 s^-1. captured primary segment: Table 4, SGZ column group, enzyme row R146K: Km 13.45 uM; kcat 0.05 s^-1; kcat/Km 0.004 uM^-1 s^-1.

normalized_value_unit_evidence: Km: printed Table 4 cell 13.45 uM; 1 uM = 1/1000 mM, so 13.45 / 1000 = 0.01345 mM; the export stores 0.01345 mM, the same value. kcat: printed Table 4 cell 0.05 s^-1; the printed turnover column is already per second, so the cell is carried across unchanged as 0.05 s^-1; the export stores 0.05 s^-1, the same value. kcat/Km: printed Table 4 cell 0.004 under the column head kcat/Km (uM^-1 s^-1); 1 uM^-1 = 1000 mM^-1, so 1 uM^-1 s^-1 = 1000 s^-1 mM^-1 and 0.004 x 1000 = 4.0 s^-1 mM^-1; the export stores 0.004 s^-1 mM^-1, which is the printed per-micromolar cell carrying the per-millimolar label without being multiplied by 1000.

raw_value_unit_evidence: Journal page 333, Table 4, enzyme row R146K, SGZ column group: Km = 13.45 uM; kcat = 0.05 s^-1; kcat/Km = 0.004 under the printed column head kcat/Km (uM^-1 s^-1).

row_label: Table 4, enzyme row R146K


sequence_variant_note: Resolved canonical sequence from UniProtKB:P07788 canonical CotA sequence, row-specific mutation reconstructed from selected mutation_signature and applied mutation signature A478F to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant A478F


sequence_variant_note: Resolved canonical sequence from UniProtKB:P07788 canonical CotA sequence, row-specific mutation reconstructed from selected mutation_signature and applied mutation signature R146K to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant R146K


sequence_variant_note: Resolved canonical sequence from UniProtKB:P07788 canonical CotA sequence, row-specific mutation reconstructed from selected mutation_signature and applied mutation signature L343S to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 4

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant L343S


sequence_variant_note: Resolved canonical sequence from UniProtKB:P07788 canonical CotA sequence, row-specific mutation reconstructed from selected mutation_signature and applied mutation signature E29V/L343S/E498G to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E29V/L343S/E498G


sequence_variant_note: Resolved canonical sequence from UniProtKB REST P07788 canonical sequence fetched 2026-05-30; F207Y applied after residue validation. and applied mutation signature F207Y to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant F207Y C-terminal His6-tagged


sequence_variant_note: Resolved canonical sequence from UniProtKB REST P07788 canonical sequence fetched 2026-05-30; V403T applied after residue validation. and applied mutation signature V403T to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant V403T C-terminal His6-tagged


sequence_variant_note: Mutation signature R416A matches the mutant residue at offset +0 and the reference residue at offset +13; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant R416A


sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.


organism: Bacteroides thetaiotaomicron

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


organism: basidiomycete PM1

assay_conditions_summary: pH 5

proof_lines: pH 5.0, temperature not specified in the publication, engineered enzyme 7D5

proof_lines: pH 3.0, temperature not specified in the publication, engineered enzyme 7D5

proof_lines: pH 4.0, temperature not specified in the publication, engineered enzyme 7D5


organism: Corynebacterium glutamicum

mutation_signature: Δ1

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) wildtype N-delta 1-111


organism: Escherichia coli

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines:
pH 5, 30°C
presence of 0.25 mM Cu2+, pH 5, 30°C
presence of 0.1 mM Cu2+, pH 5, 30°C


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant deletion aa367-416, P444G

proof_lines: region Pro357-His406 deletion plus P444G mutant, pH 5.5, 25°C


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant deletion aa367-416


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant P444A

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant deletion aa367-416, P444A


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant D439A, P444A

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant deletion aa367-416, D439A, P444A

proof_lines: region Pro357-His406 deletion plus D439A/P444A mutant, pH 5.5, 25°C


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant D439A

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant deletion aa367-416, D439A


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant P444L

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant deletion aa367-416, P444L


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant C-terminal addition of 21 methionine rich tail, N-terminal His6-tag

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant delta alpha5-7 (amino acids 357-406 deleted), C-terminal His6-tag


mutation_signature: laccase(Enzyme) mutant C-terminal addition of 21 methionine rich tail / deletion residues 355-398

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant C-terminal addition of 21 methionine rich tail, deletion residues 355-398, N-terminal His6-tag


substrate_name: O2; 2,6-Dimethoxyphenol

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant deletion residues 355-398, N-terminal His6-tag

substrate: O2; 2,6-Dimethoxyphenol


sequence_source: UniProtKB P36649 canonical sequence fetched 2026-05-30; source mutation G276K interpreted against the UniProt mature chain 29-516, so mature residue 276 maps to canonical residue 304

sequence_variant_note: Mutation signature G276K could not be applied safely because the resolved sequence does not match the expected reference residue(s).

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant G276K


sequence_source: UniProtKB P36649 canonical sequence fetched 2026-05-30; source mutation G276R interpreted against the UniProt mature chain 29-516, so mature residue 276 maps to canonical residue 304

sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset +4, so it is treated as a source-provided variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant G276R


sequence_source: UniProtKB P36649 canonical sequence fetched 2026-05-30; source mutation G276Y interpreted against the UniProt mature chain 29-516, so mature residue 276 maps to canonical residue 304

sequence_variant_note: Mutation signature G276Y could not be applied safely because the resolved sequence does not match the expected reference residue(s).

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant G276Y


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant G276N


organism: Geobacillus sp. WSUCF1


organism: Marinomonas mediterranea

mutation_signature: truncated MmPPOA-637

mutation_type: truncation

proof_lines: SABIO-RK modifier species: laccase(Enzyme) wildtype C-terminal His6-tag, truncated MmPPOA-637


organism: Klebsiella sp. 601

sequence_source: Reconstructed from direct UniProt B0FMT2 canonical sequence plus PMID 21624144 alpha351-380M substitutions; not accepted because the selected batch row aggregates multiple substrate source rows.

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant alpha351-380M


sequence_source: Reconstructed from direct UniProt B0FMT2 canonical sequence with E106F substitution confirmed by PMID 21624144; accepted status still blocked by exact kinetic tuple verification.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E106F


organism: Haloferax volcanii

substrate_name: bilirubin

substrate: bilirubin


substrate_name: syringaldazine

substrate: syringaldazine


proof_lines: recombinant His-tagged enzyme MmPPOA-695, pH 5.0, 25°C


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed entry P07788 (COTA_BACSU) plus direct single-substitution reconstruction from the selected mutation signature. and applied mutation signature R476K to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant R476K


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed entry P07788 (COTA_BACSU) plus direct single-substitution reconstruction from the selected mutation signature. and applied mutation signature T480A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant T480A


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed entry P07788 (COTA_BACSU) plus direct single-substitution reconstruction from the selected mutation signature. and applied mutation signature T480F to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant T480F


organism: Paenibacillus glucanolyticus

sequence_source: UniProtKB:A0A165SC98 direct REST lookup on 2026-05-30


organism: Ochrobactrum sp. 531


organism: Pleurotus ostreatus

substrate_name: 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid); O2; 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid); O2; H2O; ...

sequence_source: UniProtKB:Q12739 FASTA retrieved 2026-05-30


substrate_name: 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid); syringaldazine; 2,6-dimethoxyphenol

mutation_signature: Δ4

proof_lines: pH 3.0, 22°C, recombinant truncated mutant POXA1bDELTA4

proof_lines: pH 6.0, 22°C, recombinant truncated mutant POXA1bDELTA4

proof_lines: pH 5.3, 22°C, recombinant truncated mutant POXA1bDELTA4


organism: Pseudomonas fluorescens


organism: Geobacillus sp. JS12


organism: Pyrobaculum aerophilum

sequence_variant_note: Resolved canonical sequence from UniProtKB:Q8ZWA8 direct REST lookup 2026-05-30; mutation reconstructed from selected SABIO note and applied mutation signature M470F to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M470F N-terminal His-tagged


organism: Rheinheimera sp.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant deltaRhLacc (367 amino acids)


organism: Streptomyces californicus

substrate_name: Potassium ferrocyanide; O2

substrate: Potassium ferrocyanide; O2


organism: Streptomyces coelicolor

sequence_source: UniProt Q9XAL8 canonical sequence with Y108F applied during 2026-05-30 worker review

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant Y108F


sequence_source: UniProt Q9XAL8 canonical sequence with Y108A applied during 2026-05-30 worker review

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant Y108A


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M168G


organism: Rhodococcus erythropolis

mutation_signature: laccase( ) deletion -terminal residues

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant deletion 21 C-terminal residues, N-terminal His6-tag


sequence_source: UniProtKB:Q8ZWA8 canonical context only; source row is M470L N-terminal His-tagged and exact construct sequence is unresolved.

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M470L N-terminal His-tagged


sequence_variant_note: Resolved canonical sequence from UniProtKB Q9XAL8 REST FASTA checked 2026-05-30; M266W reconstructed with shared +30 residue-numbering offset inferred from selected batch signatures. and applied mutation signature M266W with inferred residue-numbering offset +30 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M266W


sequence_variant_note: Resolved canonical sequence from UniProtKB Q9XAL8 REST FASTA checked 2026-05-30; M266A reconstructed with shared +30 residue-numbering offset inferred from selected batch signatures. and applied mutation signature M266A with inferred residue-numbering offset +30 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M266A


sequence_variant_note: Resolved canonical sequence from UniProtKB Q9XAL8 REST FASTA checked 2026-05-30; M168G/M266A reconstructed with shared +30 residue-numbering offset inferred from selected batch signatures. and applied mutation signature M168G/M266A with inferred residue-numbering offset +30 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M168G/M266A


sequence_variant_note: Resolved canonical sequence from UniProtKB Q9XAL8 REST FASTA checked 2026-05-30; M168A reconstructed with shared +30 residue-numbering offset inferred from selected batch signatures. and applied mutation signature M168A with inferred residue-numbering offset +30 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M168A


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant Y199W


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M168G/M266W


sequence_source: reconstructed_from_uniprot_accession Q9XAL8, checked 2026-05-30

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant SLAC V290A


organism: Streptomyces cyaneus

sequence_source: UniProtKB REST direct lookup F6L7B5, checked 2026-05-30

proof_lines: pH and temperature not specified in the publication


sequence_source: UniProtKB Q9XAL8 FASTA direct check; reconstructed S292A after confirming canonical residue S at position 292.

sequence_variant_note: Mutation signature S292A matches the mutant residue at offset +0 and the reference residue at offset +2; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant S292A


sequence_source: UniProtKB Q9XAL8 FASTA direct check; reconstructed M298F after confirming canonical residue M at position 298.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant SLAC M298F


sequence_source: UniProtKB Q9XAL8 FASTA direct check; reconstructed V290N after confirming canonical residue V at position 290.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant SLAC V290N


organism: Streptomyces lividans


organism: Streptomyces sviceus

sequence_variant_note: Mutation signature M220L matches the mutant residue at offset +0 and the reference residue at offset +12; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M220L, N-terminal His6-tag


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M295F Ssl1


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M293G Ssl1


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M295A Ssl1


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M293G/M295L Ssl1


sequence_source: UniProtKB B5HSR1 canonical sequence checked 2026-05-30; variant reconstructed from M295Y after matching the canonical residue(s).

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M295Y Ssl1


sequence_source: UniProtKB B5HSR1 canonical sequence checked 2026-05-30; variant reconstructed from M195A after matching the canonical residue(s).

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M195A Ssl1


sequence_source: UniProtKB B5HSR1 canonical sequence checked 2026-05-30; variant reconstructed from M295I after matching the canonical residue(s).

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M295I Ssl1


sequence_variant_note: Resolved canonical sequence from UniProtKB B5HSR1 canonical sequence reconstructed with selected mutation_signature and applied mutation signature M295T to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M295T Ssl1


sequence_variant_note: Resolved canonical sequence from UniProtKB B5HSR1 canonical sequence reconstructed with selected mutation_signature and applied mutation signature V287N to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant V287N Ssl1


sequence_variant_note: Resolved canonical sequence from UniProtKB B5HSR1 canonical sequence reconstructed with selected mutation_signature and applied mutation signature M295V to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M295V Ssl1


sequence_variant_note: Resolved canonical sequence from UniProtKB B5HSR1 canonical sequence reconstructed with selected mutation_signature and applied mutation signature M195A/V287N/M293G/M295L to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M195A/V287N/M293G/M295L Ssl1


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


sequence_source: UniProtKB:B5HSR1 canonical sequence only

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M293L, N-terminal His6-tag


sequence_source: UniProtKB B5HSR1 direct lookup resolved the canonical protein, but the selected SABIO-RK note includes an N-terminal His6-tagged M220L/T222L construct and the exact tagged construct sequence was not provided.

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M220L/T222L, N-terminal His6-tag


sequence_source: UniProtKB B5HSR1 direct lookup resolved the canonical protein, but the selected SABIO-RK note includes an N-terminal His6-tagged M195L construct and the exact tagged construct sequence was not provided.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M195L, N-terminal His6-tag


sequence_source: UniProtKB B5HSR1 direct lookup resolved the canonical protein and residue 295=M, but the selected SABIO-RK context includes an N-terminal His6-tagged M295L note and a separate M295L Ssl1 note across two PMID/substrate contexts.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M295L , N-terminal His6-tag

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M295L Ssl1


sequence_source: UniProtKB B5HSR1 direct lookup resolved a 325-aa canonical sequence, but the selected SABIO-RK note says deletion of C-terminal residues 312-328 with an N-terminal His6 tag, leaving the coordinate system and exact construct sequence unresolved.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant del C-terminal residues 312-328, N-terminal His6-tag


sequence_source: UniProtKB B5HSR1 FASTA fetched 2026-05-30; M195A/M293G/M295L reconstructed from canonical residues M195, M293, and M295.

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M195A/M293G/M295L Ssl1


organism: Streptomyces viridochromogenes

sequence_source: UniProtKB J9S5G3 FASTA fetched 2026-05-30.

proof_lines: pH 4.0, temperature not specified in the publication

proof_lines: pH 8.5, temperature not specified in the publication


organism: Streptomyces viridosporus

sequence_source: UniProtKB J9PBR2 FASTA fetched 2026-05-30.

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.


organism: Thermus thermophilus

substrate_name: O2; Guaiacol

sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:F6DF14 and applied mutation signature D106L to reconstruct the assayed variant sequence.

column_label: Table 1: Apparent steady-state kinetic constants measured at the saturating concentrations of O2 for guaiacol by the lacTT proteins (Appl Microbiol Biotechnol 2018, 102:4049-4061); printed column heads LacTT | Km (uM) | kcat (s^-1) | kcat/Km (s^-1 mM^-1); enzyme row D106L; column 'Km (uM)' = 488; column 'kcat (s^-1)' = 2.1; column 'kcat/Km (s^-1 mM^-1)' = 4.3. captured primary segment: Table 1 row D106L | construct lacTT D106L | substrate guaiacol | condition pH 6.0 | Km 488 uM | kcat 2.1 s^-1 | kcat/Km 4.3 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 1 cell 488 uM; 488 / 1000 = 0.488 mM. kcat: printed Table 1 cell 2.1 s^-1; the printed turnover column is per second, so no unit conversion applies and the value is 2.1 s^(-1). kcat/Km: printed Table 1 cell 4.3 s^-1 mM^-1; the printed efficiency column is already per second per millimolar, so no unit conversion applies and the value is 4.3 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 1, enzyme row D106L: Km = 488 uM; kcat = 2.1 s^-1; kcat/Km = 4.3 s^-1 mM^-1.

row_label: Table 1, enzyme row D106L

substrate: O2; Guaiacol


substrate_name: Guaiacol; O2

sequence_variant_note: Resolved canonical sequence from UniProtKB:F6DF14 FASTA; direct REST lookup on 2026-05-30 and applied mutation signature E456L to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E456L

substrate: Guaiacol; O2


sequence_variant_note: Resolved canonical sequence from UniProtKB:F6DF14 FASTA; direct REST lookup on 2026-05-30 and applied mutation signature D423A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant D423A


sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


sequence_source: UniProtKB:Q72HW2 direct lookup 2026-05-30

proof_lines: pH and temperature not specified in the publication


organism: Streptomyces griseoflavus

sequence_source: UniProt A0A0M4FJ81 canonical sequence fetched via UniProt REST on 2026-05-30; row mutation reconstructed during review.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant H165F


organism: Thermobaculum terrenum


organism: Thermobifida fusca

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant R49K, N-terminal His-tag


organism: Thermus sp.


sequence_source: UniProtKB REST accession F6DF14, checked 2026-05-30; row-specific variant reconstructed from canonical sequence

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E356A


sequence_variant_note: Mutation signature D106R matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant D106R


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:F6DF14 and applied mutation signature E456D to reconstruct the assayed variant sequence.

column_label: Table 1: Apparent steady-state kinetic constants measured at the saturating concentrations of O2 for guaiacol by the lacTT proteins (Appl Microbiol Biotechnol 2018, 102:4049-4061); printed column heads LacTT | Km (uM) | kcat (s^-1) | kcat/Km (s^-1 mM^-1); enzyme row E456D; column 'Km (uM)' = 380; column 'kcat (s^-1)' = 0.78; column 'kcat/Km (s^-1 mM^-1)' = 2.1. captured primary segment: Table 1 row E456D | construct lacTT E456D | substrate guaiacol | condition pH 6.0 | Km 380 uM | kcat 0.78 s^-1 | kcat/Km 2.1 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 1 cell 380 uM; 380 / 1000 = 0.38 mM. kcat: printed Table 1 cell 0.78 s^-1; the printed turnover column is per second, so no unit conversion applies and the value is 0.78 s^(-1). kcat/Km: printed Table 1 cell 2.1 s^-1 mM^-1; the printed efficiency column is already per second per millimolar, so no unit conversion applies and the value is 2.1 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 1, enzyme row E456D: Km = 380 uM; kcat = 0.78 s^-1; kcat/Km = 2.1 s^-1 mM^-1.

row_label: Table 1, enzyme row E456D


sequence_variant_note: Mutation signature D106E matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant D106E


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:F6DF14 and applied mutation signature E456R to reconstruct the assayed variant sequence.

column_label: Table 1: Apparent steady-state kinetic constants measured at the saturating concentrations of O2 for guaiacol by the lacTT proteins (Appl Microbiol Biotechnol 2018, 102:4049-4061); printed column heads LacTT | Km (uM) | kcat (s^-1) | kcat/Km (s^-1 mM^-1); enzyme row E456R; column 'Km (uM)' = 512; column 'kcat (s^-1)' = 0.32; column 'kcat/Km (s^-1 mM^-1)' = 0.62. captured primary segment: Table 1 row E456R | construct lacTT E456R | substrate guaiacol | condition pH 6.0 | Km 512 uM | kcat 0.32 s^-1 | kcat/Km 0.62 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 1 cell 512 uM; 512 / 1000 = 0.512 mM. kcat: printed Table 1 cell 0.32 s^-1; the printed turnover column is per second, so no unit conversion applies and the value is 0.32 s^(-1). kcat/Km: printed Table 1 cell 0.62 s^-1 mM^-1; the printed efficiency column is already per second per millimolar, so no unit conversion applies and the value is 0.62 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 1, enzyme row E456R: Km = 512 uM; kcat = 0.32 s^-1; kcat/Km = 0.62 s^-1 mM^-1.

row_label: Table 1, enzyme row E456R


sequence_source: UniProtKB:F6DF14 canonical sequence with M460Q reconstructed during exact accession lookup

sequence_variant_note: Mutation signature M460Q matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant LacTT M460Q , His-tagged


sequence_source: UniProtKB:F6DF14 canonical sequence with M460H reconstructed during exact accession lookup

sequence_variant_note: Mutation signature M460H matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant LacTT M460H, His-tagged


sequence_source: UniProt REST accession F6L7B5, checked 2026-05-30


substrate_name: Guaiacol

substrate: Guaiacol


sequence_variant_note: Resolved canonical sequence from UniProt REST accession A0A0M4FJ81 plus H165A substitution reconstructed against the canonical sequence, checked 2026-05-30 and applied mutation signature H165A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant H165A


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:A0A0M4FJ81 and applied mutation signature I170A to reconstruct the assayed variant sequence.

column_label: Table 3 (Kinetic parameters of the SgfSL variants), ABTS block, protein row 'I170A'; column 'Km (mM)' = 0.63 ± 0.11; column 'kcat (s-1)' = 0.70 ± 0.20; column 'kcat/Km (mM-1 s-1)' = 1.11; column 'Activity nM O2 min-1 ug-1' = 0.11 ± 0.01.

normalized_value_unit_evidence: kcat: printed Table 3 cell 0.70 under the column head kcat (s-1); the cell is already in s^-1, so no unit conversion applies and the canonical value is 0.7 s^(-1). Km: printed Table 3 cell 0.63 under the column head Km (mM); the cell is already in mM, so no unit conversion applies and the canonical value is 0.63 mM. kcat/Km: printed Table 3 cell 1.11 under the column head kcat/Km (mM-1 s-1); the cell is already in mM^-1 s^-1, so no unit conversion applies and the canonical value is 1.11 s^-1 mM^-1.

raw_value_unit_evidence: Table 3, ABTS block, protein row 'I170A': Km = 0.63 mM; kcat = 0.70 s-1; printed kcat/Km = 1.11 mM-1 s-1.

table_label: Table 3: Kinetic parameters of the SgfSL variants (Int. J. Mol. Sci. 2019, volume 20, article 3184, page 6); printed column heads Substrate | Protein | Km (mM) | kcat (s-1) | kcat/Km (mM-1 s-1) | Activity nM O2 min-1 ug-1


sequence_source: UniProt REST accession A0A0M4FJ81 canonical sequence, checked 2026-05-30


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:A0A0M4FJ81 and applied mutation signature I170F to reconstruct the assayed variant sequence.

column_label: Table 3 (Kinetic parameters of the SgfSL variants), ABTS block, protein row 'I170F'; column 'Km (mM)' = 0.20 ± 0.04; column 'kcat (s-1)' = 0.08 ± 0.01; column 'kcat/Km (mM-1 s-1)' = 0.40; column 'Activity nM O2 min-1 ug-1' = 0.08 ± 0.04.

normalized_value_unit_evidence: kcat: printed Table 3 cell 0.08 under the column head kcat (s-1); the cell is already in s^-1, so no unit conversion applies and the canonical value is 0.08 s^(-1). Km: printed Table 3 cell 0.20 under the column head Km (mM); the cell is already in mM, so no unit conversion applies and the canonical value is 0.2 mM. kcat/Km: printed Table 3 cell 0.40 under the column head kcat/Km (mM-1 s-1); the cell is already in mM^-1 s^-1, so no unit conversion applies and the canonical value is 0.4 s^-1 mM^-1.

raw_value_unit_evidence: Table 3, ABTS block, protein row 'I170F': Km = 0.20 mM; kcat = 0.08 s-1; printed kcat/Km = 0.40 mM-1 s-1.


organism: Toxicodendron vernicifluum

substrate_name: ferrocyanide

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

substrate: ferrocyanide


organism: Trametes cinnabarina

proof_lines: recombinant wild-type enzyme, pH 5.0, temperature not specified in the publication

proof_lines: recombinant wild-type enzyme, pH 3.0, temperature not specified in the publication


sequence_source: UniProtKB Q72HW2 REST FASTA fetched 2026-05-30 as canonical context only; source note says R453T, C-terminal His-tag, but the tag sequence is not specified.

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant R453T, C-terminal His-tag


sequence_source: UniProtKB REST accession Q4H436, checked 2026-05-30


sequence_source: UniProtKB REST accession F6DF14, checked 2026-05-30


organism: Trametes hirsuta

substrate_name: O2; Potassium ferrocyanide

substrate: O2; Potassium ferrocyanide


organism: Trametes sp. C30

sequence_source: UniProtKB:Q6TH77 canonical context only

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant C-terminal 13 residues deleted, His6-tag

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant C-terminal 13 residues deleted, hyperglycosated, His6-tag


organism: Trametes versicolor

substrate_name: Methyl syringate; O2

sequence_source: UniProtKB:Q12718 direct REST lookup on 2026-05-30 (https://rest.uniprot.org/uniprotkb/Q12718.json)

substrate: Methyl syringate; O2


organism: unknown prokaryotic organism

sequence_source: Canonical parent from GenBank:GQ468313.1 / protein_id ACV83921.1; Lac3T93 row-specific variant sequence unresolved.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant Lac3T93 (N40S, V55A, F62L, E316V) His-tagged


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant Lac15D (del323-332)


organism: Pleurotus ostreatus

proof_lines:
pH 7.0, 25°C
pH 6.0, 25°C
25°C, isozyme POXA3a


sequence_variant_note: Resolved canonical sequence from UniProtKB F6DF14 canonical sequence fetched directly; M460L reconstructed after checking residue 460 is methionine. and applied mutation signature M460L to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 6

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant LacTT M460L, His-tagged


sequence_variant_note: Resolved canonical sequence from UniProtKB F6DF14 canonical sequence fetched directly; M460A reconstructed after checking residue 460 is methionine. and applied mutation signature M460A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant LacTT M460A, His-tagged


sequence_variant_note: Resolved canonical sequence from UniProtKB F6DF14 canonical sequence fetched directly; M460F reconstructed after checking residue 460 is methionine. and applied mutation signature M460F to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant LacTT M460F, His-tagged


organism: Achromobacter xylosoxidans


organism: Acremonium sp.


organism: Agaricus bisporus


organism: Alcaligenes faecalis


organism: Alkalihalobacillus ligniniphilus


organism: Aquifex aeolicus

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant V19A


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant M449T


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant 1D11 (K245R-I441L-M449T-R465G-P58S-Y172C-I199T) -F55S


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant 2B3 (F55S-F17S-V19A-P58S-Y172C-I199T-R465G-K245R-I441L-M449T)


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant 2G6 (K245R-I441L-M449T-R465G) -I199T-Y172C


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant 2G6 (K245R-I441L-M449T-R465G) -Y172C


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant 1D11 (K245R-I441L-M449T-R465G-P58S-Y172C-I199T) -V19A


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant 2G6 (K245R-I441L-M449T-R465G) -P58S


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant 2G6 (K245R-I441L-M449T-R465G)


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant R471G


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant 1D11 (K245R-I441L-M449T-R465G-P58S-Y172C-I199T)


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant P58S


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant I441L


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant K245R


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant Y172C


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant 2G6 (K245R-I441L-M449T-R465G) -I199T


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant F55S


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant 2G6 (K245R-I441L-M449T-R465G) -I199T-P58S


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant 1D11 (K245R-I441L-M449T-R465G-P58S-Y172C-I199T) -F17S


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant 1B7 (K245R-I441L-M449T)


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant I199T


organism: Bacillus amyloliquefaciens

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant T317N-L386Y-S427E


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant T317N-L386Y-A110E


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant T317N-L386Y


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant T317N-L386Y-S427E-A110E


organism: Bacillus atrophaeus


organism: Bacillus coagulans


organism: Bacillus pumilus

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant L2 (L386Q, G417I, +SP)


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant L9 (L386Q, G417R, V482G)


proof_lines: pH 7.0, 22°C, recombinant enzyme, with 25% v/v DMSO

proof_lines: pH 6.5, 22°C, recombinant enzyme, with 0.8% v/v DMSO


organism: Bacillus sp.


organism: Bacillus subtilis

sequence_source: UniProtKB P07788 canonical CotA sequence used as identity context for the unmutated holoCotA record.

proof_lines:
pH 7, holoCotA
pH 3, holoCotA
pH 7.5, 37°C


organism: Bacillus tequilensis


organism: Carica papaya


organism: Corynebacterium efficiens


organism: Desulfovibrio sp. A2


organism: Enterobacter ludwigii


organism: Citrobacter freundii


organism: Mycothermus thermophilus


organism: Myrioconium sp.


organism: Myrothecium verrucaria


organism: Obba rivulosa

substrate_name: guaiacol; 2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate); syringaldazine; 2,6-dimethoxyphenol

proof_lines:
pH 3.0, 25 C, laccase Lac-4.8
pH 3.0, 25 C, laccase Lac-3.5
pH 3.0, 25 C,laccase Lac-4.8


organism: Perenniporia fraxinea


organism: Lysobacter sp. T-15


organism: Lactobacillus plantarum


organism: Lepista nuda


organism: Pseudomonas sp. LBC1

substrate_name: O2; 3,3'-Dimethylbenzidine

substrate: O2; 3,3'-Dimethylbenzidine


organism: Shewanella putrefaciens


organism: Shigella dysenteriae


proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant E106F, deletion L351-G378


organism: Pleurotus nebrodensis


organism: Sporothrix carnis


organism: Streptomyces anulatus


organism: Streptomyces griseorubens


organism: Thielavia sp.


organism: Thioalkalivibrio sp.


organism: Trametes hirsuta

proof_lines: pH 4.5, 50°C, enzyme conjugated with 1.1 kDa PEG


organism: Trametes polyzona

proof_lines: pH 5.0, temperature not specified in the publication

proof_lines: pH and temperature not specified in the publication