organism: Salmonella enterica

assay_conditions_summary: pH 8; 298.15 K; cofactor ATP

proof_lines: pH 8.0, 25°C, recombinant CobA mutant F91W/W93F


enzyme_display_name: corrinoid adenosyltransferase

substrate_name: ATP

source_db: brenda

ki_display: Not reported

kcat_over_km_display: Not reported

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution

proof_lines: pH 8.0, 25°C, recombinant CobA mutant W93A

enzyme: corrinoid adenosyltransferase

substrate: ATP

source: brenda


proof_lines: pH 8.0, 25°C, recombinant CobA mutant W93F


proof_lines: pH 8.0, 25°C, recombinant CobA mutant F91A


enzyme_display_name: thymidylate synthase

organism: Cryptococcus neoformans

kcat_display: Not reported

mutation_signature: Unknown

protein_accession_database: UniProt

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

enzyme: thymidylate synthase


organism: Enterococcus faecalis

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Escherichia coli


proof_lines: 10fold higher than wild-type thymidylate synthase

proof_lines: 30fold higher than wild-type thymidylate synthase


substrate_name: methylenetetrahydropteroylgluamate

substrate: methylenetetrahydropteroylgluamate


substrate_name: methylenetetrahydrofolate

substrate: methylenetetrahydrofolate


substrate_name: Unknown

km_display: Not reported

ki_unit: mM

substrate: Unknown


substrate_name: dUMP

substrate: dUMP


organism: Homo sapiens


proof_lines:
wild type enzyme, at 37°C
wild type


proof_lines:
presence of Mg2+, pH 7.5, 22°C
pH 7.5, 22°C
pH 7.2, 22°C


organism: Mycobacterium tuberculosis


organism: Penaeus vannamei


organism: Plasmodium falciparum


proof_lines: Ala-FACE mutant enzyme with residues 284, 285, 288, 289, and 292 mutated to Ala


organism: Pneumocystis carinii


organism: Shrimp white spot syndrome virus


organism: Trypanosoma brucei


enzyme_display_name: kynurenine--oxoglutarate transaminase

organism: Aedes aegypti

substrate_name: L-asparagine

enzyme: kynurenine--oxoglutarate transaminase

substrate: L-asparagine


organism: Haemaphysalis longicornis

assay_conditions_summary: pH 7.5; 310.15 K; buffer 200 mM phosphate

proof_lines: in 200 mM phosphate buffer, pH 7.5, at 37°C

proof_lines: in 200 mM phosphate buffer, pH7.5, at 37°C


assay_conditions_summary: pH 7.4; 310.15 K; buffer 100 mM potassium phosphate

proof_lines: in 100 mM potassium phosphate buffer (pH 7.4), at 37°C

proof_lines: mass spectrometry assay, pH 7.5, temperature not specified in the publication

proof_lines: pH and temperature not specified in the publication


substrate_name: L-kynurenine

substrate: L-kynurenine


organism: Mus musculus

substrate_name: L-cysteine

assay_conditions_summary: pH 9; 318.15 K; buffer 100 mM boric acid

proof_lines: in 100 mM boric acid buffer, pH 9.0, at 45°C

substrate: L-cysteine


substrate_name: hydroxyphenylpyruvate

substrate: hydroxyphenylpyruvate


organism: Rattus norvegicus

substrate_name: pyruvate

substrate: pyruvate


proof_lines: pH 8.0, 37°C, recombinant mitochondrial enzyme


proof_lines: pH 8.0, 37°C, recombinant enzyme, cosubstrate L-kyrunenine

proof_lines: pH 8.0, 37°C, alpha-aminoadipate aminotransferase activity


organism: Unknown


enzyme_display_name: cysteine synthase

organism: Arabidopsis thaliana

substrate_name: Sulfide

enzyme: cysteine synthase

substrate: Sulfide


enzyme_display_name: firefly luciferase

organism: Photinus pyralis

substrate_name: D-luciferin

proof_lines:
mutant enzyme G246A
pH 8.6, mutant enzyme G246A

enzyme: firefly luciferase

substrate: D-luciferin


organism: Bos taurus

substrate_name: Inosine

substrate: Inosine


enzyme_display_name: 4-hydroxyphenylpyruvate dioxygenase

organism: Avena sativa

enzyme: 4-hydroxyphenylpyruvate dioxygenase


organism: Limosilactobacillus reuteri

substrate_name: cob(II)alamin

assay_conditions_summary: cofactor ATP

proof_lines:
mutant F112Y, Co2+ assay
mutant F112Y, Co+ assay

substrate: cob(II)alamin


substrate_name: cob(I)alamin

proof_lines:
wild-type, Co+ assay
wild-type, Co2+ assay

substrate: cob(I)alamin


proof_lines:
mutant F187A, Co2+ assay
mutant F187A, Co+ assay


organism: Salmonella enterica

proof_lines: pH 8.0, 25°C, recombinant CobA mutant F91Y


proof_lines: pH 8.0, 25°C, recombinant CobA mutant W93Y


proof_lines: pH 8.0, 25°C, recombinant CobA mutant F91W


proof_lines: pH 8.0, 25°C, recombinant CobA mutant F91H


enzyme_display_name: orotidine-5'-phosphate decarboxylase

organism: Escherichia coli

substrate_name: orotidine 5'-phosphate

enzyme: orotidine-5'-phosphate decarboxylase

substrate: orotidine 5'-phosphate


proof_lines:
mutant enzyme F250S
pH 8.6, mutant enzyme F250S


enzyme_display_name: aralkylamine N-acetyltransferase

organism: Drosophila melanogaster

assay_conditions_summary: pH 8; 295.15 K; cofactor CoA

proof_lines: cosubstrate hexanoyl-CoA, wild-type, pH 8.0, 22°C

proof_lines: cosubstrate butanoyl-CoA, wild-type, pH 8.0, 22°C

enzyme: aralkylamine N-acetyltransferase


substrate_name: acetyl-CoA

substrate: acetyl-CoA


substrate_name: histamine

substrate: histamine


substrate_name: ATP

substrate: ATP


proof_lines: recombinant mutant Q358E, pH and temperature not specified in the publication


proof_lines: recombinant mutant N261D, pH and temperature not specified in the publication


proof_lines: recombinant mutant Q286E, pH and temperature not specified in the publication


proof_lines: recombinant mutant S246A, pH and temperature not specified in the publication


substrate_name: methylenetetrahydrofolate

substrate: methylenetetrahydrofolate


substrate_name: tryptamine

substrate: tryptamine


enzyme_display_name: chitinase

organism: Vibrio harveyi

substrate_name: chitin

proof_lines: pH 5.5, 37°C, colloidal chitin, mutant enzym W70A

enzyme: chitinase

substrate: chitin


proof_lines: pH 5.5, 37°C, colloidal chitin, mutant enzym W231F


substrate_name: chitohexaose

proof_lines: pH 5.5, 37°C, colloidal chitin, mutant enzym W231A

substrate: chitohexaose


proof_lines: pH 5.5, 37°C, colloidal chitin, mutant enzym S33W


enzyme_display_name: subtilisin

organism: Bacillus amyloliquefaciens

substrate_name: Unknown

enzyme: subtilisin

substrate: Unknown


enzyme_display_name: tryptophan synthase

organism: Mycobacterium tuberculosis

substrate_name: indole

enzyme: tryptophan synthase

substrate: indole


organism: Streptomyces avermitilis


proof_lines: pH 7.0, 25°C, wild-type enzyme and mutant N245I


organism: Homo sapiens

substrate_name: dUMP

proof_lines: apparent value, mutant enzyme V3F, pH not specified in the publication, at 37°C

substrate: dUMP


proof_lines: apparent value, mutant enzyme V3L, pH not specified in the publication, at 37°C


proof_lines: apparent value, mutant enzyme V3Y, pH not specified in the publication, at 37°C


proof_lines: apparent value, mutant enzyme V3T, pH not specified in the publication, at 37°C


organism: Niallia circulans

substrate_name: carboxymethyl chitin

substrate: carboxymethyl chitin


organism: Dichelobacter nodosus


organism: Saccharomyces cerevisiae


substrate_name: 1-(beta-D-erythrofuranosyl)orotic acid

substrate: 1-(beta-D-erythrofuranosyl)orotic acid


substrate_name: D-luciferin

proof_lines: mutant I423L, in the presence of 0.01 mM ATP

proof_lines: mutant I423L, in the presence of 0.001 mM D-luciferin

substrate: D-luciferin


proof_lines: mutant I423L/D436G/L530R, in the presence of 0.001 mM D-luciferin

proof_lines: mutant I423L/D436G/L530R, in the presence of 0.01 mM ATP


proof_lines: mutant D436G, in the presence of 0.01 mM ATP

proof_lines: mutant D436G, in the presence of 0.001 mM D-luciferin


proof_lines: mutant L530R, in the presence of 0.01 mM ATP

proof_lines: mutant L530R, in the presence of 0.001 mM D-luciferin


substrate_name: Luciferin

substrate: Luciferin


proof_lines:
mutant F112W, Co+ assay
mutant F112W, Co2+ assay


mutation_type: deletion

proof_lines:
mutant DELTAS183, Co2+ assay
mutant DELTAS183, Co+ assay


substrate_name: cob(II)alamin

proof_lines:
mutant F163A, Co2+ assay
mutant F163A, Co+ assay

substrate: cob(II)alamin


proof_lines:
mutant F112H, Co2+ assay
mutant F112H, Co+ assay


substrate_name: cob(I)alamin

proof_lines: pH 8.0, 25°C, recombinant CobA mutant W93H

substrate: cob(I)alamin


proof_lines: pH 8.0, 25°C, recombinant CobA mutant W93D


proof_lines: pH 8.0, 25°C, recombinant CobA mutant F91A/W93D


proof_lines: pH 8.0, 25°C, recombinant wild-type CobA

proof_lines: pH 8.0, 37°C, recombinant full length wild-type enzyme


enzyme_display_name: aryl sulfotransferase

proof_lines: SULT1A3 double mutant H143Y/E146A, pH 7.4, 37°C

enzyme: aryl sulfotransferase


proof_lines:
wild-type SULT1A3, pH 7.4, 37°C
wild-type SULT1A1, pH 7.4, 37°C
wild-type SULT1A1


organism: Lederbergia lenta

substrate_name: succinyl-AAPF-p-nitroanilide

proof_lines: pH 7.5, 25°C mutant S166C-S-d and M222C-S-d

substrate: succinyl-AAPF-p-nitroanilide


proof_lines:
pH 7.5, 25°C mutant M222C-S-f
pH 7.5, 25°C mutant M222C-S-e
pH 7.5, 25°C mutant M222C-S-a


proof_lines:
pH 7.5, 25°C mutant S156C-S-c
pH 7.5, 25°C mutant S156C-S-e
pH 7.5, 25°C mutant S156C-S-f


proof_lines: pH 7.5, 25°C mutant S156C-S-c and S166C-S-f


enzyme_display_name: penicillin amidase

organism: Alcaligenes faecalis

substrate_name: benzylpenicillin

proof_lines: pH 7.5, 25°C, mutant enzyme T206G and wild-type enzyme

enzyme: penicillin amidase

substrate: benzylpenicillin


proof_lines: pH 7.5, 25°C, mutant enzyme T206G//S213G


proof_lines: pH 7.5, 25°C, mutant enzyme T206G/S213G/T219G


substrate_name: orotidine 5'-phosphate

substrate: orotidine 5'-phosphate


organism: Photinus pyralis


assay_conditions_summary: pH 8.5; 308.15 K; cofactor ATP


substrate_name: ATP

assay_conditions_summary: pH 8; 308.15 K; cofactor ATP

substrate: ATP


assay_conditions_summary: pH 8.5; 298.15 K; cofactor ATP


organism: Pyrophorus plagiophthalamus

substrate_name: luciferyl-O-adenosine monophosphate

substrate: luciferyl-O-adenosine monophosphate


organism: Lacticaseibacillus casei


proof_lines:
mutant TS N229D
mutant TS N229D, dCMP as substrate


substrate_name: histamine

substrate: histamine


organism: Limosilactobacillus reuteri

proof_lines: mutant D35R/R128D, at subsaturating concentrations of 30 mM ATP and 0.02 mM cob(I)alamin

proof_lines: mutant D35R/R128D, at subsaturating concentrations of ATP 30 mM ATP and 0.02 mM cob(I)alamin


substrate_name: O-acetyl-L-serine

substrate: O-acetyl-L-serine


organism: Bos taurus

substrate_name: Inosine

substrate: Inosine


organism: Hevea brasiliensis


proof_lines:
30°C, pH 4.2, purified wild-type
30°C, pH 4.2, recombinant wild-type


organism: Oryza sativa

substrate_name: reduced N-acetylchitopentaose

substrate: reduced N-acetylchitopentaose


organism: Vibrio harveyi

substrate_name: p-nitrophenyl-N,N'-diacetylchitobiose

substrate: p-nitrophenyl-N,N'-diacetylchitobiose


enzyme_display_name: (S)-hydroxynitrile lyase

organism: Baliospermum montanum

substrate_name: 1-naphthalene carboxaldehyde

enzyme: (S)-hydroxynitrile lyase

substrate: 1-naphthalene carboxaldehyde


substrate_name: (R)-mandelonitrile

substrate: (R)-mandelonitrile


proof_lines: pH 4.23, 25°C, mutant enzyme H103C/N156G


proof_lines: pH 4.23, 25°C, mutant enzyme H103C/N156D


assay_conditions_summary: pH 5

proof_lines: mutant enzyme F125T/Y133F, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme F125T/Y133F


proof_lines: mutant enzyme L121Y/F125T/Y133F, pH and temperature not specified in the publication


substrate_name: mandelonitrile

proof_lines: mutant enzyme F125T, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme F125T

substrate: mandelonitrile


proof_lines: mutant enzyme L121Y, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121Y


proof_lines: mutant enzyme V106F/L121Y/F125T, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y/F125T


proof_lines: mutant enzyme L146M, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L146M


proof_lines: mutant enzyme I209A, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme I209A


substrate_name: rac-mandelonitrile

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121A

substrate: rac-mandelonitrile


enzyme_display_name: carnitine O-palmitoyltransferase

substrate_name: L-carnitine

assay_conditions_summary: cofactor CoA

enzyme: carnitine O-palmitoyltransferase

substrate: L-carnitine


substrate_name: palmitoyl-CoA

substrate: palmitoyl-CoA


proof_lines: P504L/V605L mutant protein, COS-7 cell lysate


proof_lines: F352C/V368I mutant protein, COS-7 cell lysate


proof_lines: F352C/V368I/V605L mutant protein, COS-7 cell lysate


assay_conditions_summary: pH 7.4; 310.15 K; cofactor CoA


assay_conditions_summary: pH 7.4; 298.15 K; cofactor CoA

proof_lines: mutant enzyme Y479F, in presence of 0.25 mM cardiolipin, at pH 7.4 and 25°C

proof_lines: mutant enzyme Y479F, in absence of cardiolipin, at pH 7.4 and 25°C


proof_lines: mutant enzyme S113L, in absence of cardiolipin, at pH 7.4 and 25°C

proof_lines: mutant enzyme S113L, in presence of 0.25 mM cardiolipin, at pH 7.4 and 25°C


proof_lines: mutant enzyme P50H, in presence of 0.25 mM cardiolipin, at pH 7.4 and 25°C

proof_lines: mutant enzyme P50H, in absence of cardiolipin, at pH 7.4 and 25°C


proof_lines: wild type enzyme, in absence of cardiolipin, at pH 7.4 and 25°C

proof_lines: wild type enzyme, in presence of 0.25 mM cardiolipin, at pH 7.4 and 25°C

proof_lines: wild-type protein and P504L/V605L mutant protein, COS-7 cell lysate (doubly transfected)


organism: Larimichthys crocea

proof_lines: 25°C, pH not specified in the publication


organism: Ovis aries

proof_lines: chimaera in which the distinctive N-terminal segment of ovine m-BPT 1 is replaced with that from rat M-CPT 1


proof_lines:
wild-type L-CPT 1
wild-type L-CPT1


proof_lines:
wild-type M-CPT 1
wild-type M-CPT1


organism: Rattus norvegicus


proof_lines:
wild-type
wild-type enzyme


organism: Sus scrofa

assay_conditions_summary: pH 7; cofactor CoA


organism: Tachysurus fulvidraco

assay_conditions_summary: pH 8; 310.15 K; cofactor CoA

proof_lines:
enzyme from muscle, at pH 8.0 and 37°C
enzyme from heart, at pH 8.0 and 37°C
enzyme from spleen, at pH 8.0 and 37°C


proof_lines: mutant enzyme L121Y/L146M, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/L146M


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121F


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121W


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121S


proof_lines: mutant enzyme V106F/F125T, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme V106F/F125T


proof_lines: mutant enzyme V106F/L146M, pH and temperature not specified in the publication


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121C


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121Q


proof_lines: mutant enzyme I209G, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme I209G


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121T


proof_lines: wild type enzyme, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, wild-type enzyme


proof_lines: mutant enzyme L121Y/F125T, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T


proof_lines: mutant enzyme Y133F, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme Y133F


proof_lines: mutant enzyme F125T/L146M, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme F125T/L146M


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121V


proof_lines: mutant enzyme L152F, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L152F


proof_lines: mutant enzyme L148F, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L148F


enzyme_display_name: phosphoenolpyruvate carboxykinase (GTP)

organism: Homo sapiens

substrate_name: GDP

proof_lines: Mg2+ concentration 5 mM, Y235S mutant. Vmax = 10 micromol/min/mg

proof_lines: Mg2+ concentration 7.5 mM, Y235S mutant. Vmax = 11 micromol/min/mg

enzyme: phosphoenolpyruvate carboxykinase (GTP)

substrate: GDP


proof_lines: Mg2+ concentration 2 mM, wild-type. Vmax = 33 micromol/min/mg

proof_lines: Mn2+ concentration 1.5 mM, Mg2+ concentration 2 mM, wild-type. Vmax = 31 micromol/min/mg


proof_lines: Mg2+ concentration 5 mM, Y235F mutant. Vmax = 3 micromol/min/mg

proof_lines: Mg2+ concentration 7.5 mM, Y235F mutant. Vmax = 3 micromol/min/mg


proof_lines: Mg2+ concentration 5 mM, Y235A mutant. Vmax = 15 micromol/min/mg

proof_lines: Mg2+ concentration 2 mM, Y235A mutant. Vmax = 15 micromol/min/mg

proof_lines: Mg2+ concentration 7.5 mM, Y235A mutant. Vmax = 15 micromol/min/mg


organism: Mycolicibacterium smegmatis

substrate_name: phosphoenolpyruvate

proof_lines:
pH 7.2, 37°C
pH 7.2, 37°C, bicarbonate

substrate: phosphoenolpyruvate


substrate_name: oxaloacetate

substrate: oxaloacetate


enzyme_display_name: phosphoenolpyruvate carboxylase

organism: Alternanthera ficoidea

proof_lines:
+ 5 mM D-glucose 6-phosphate
without 5 mM D-glucose 6-phosphate

enzyme: phosphoenolpyruvate carboxylase


organism: Alternanthera pungens


organism: Alternanthera sessilis

proof_lines:
+ 5 mM glucose 6-phosphate
without 5 mM glucose 6-phosphate


organism: Clostridium perfringens

assay_conditions_summary: pH 7.2; 310.15 K; buffer 50 mM HEPES-NaOH

proof_lines: in the presence of 2 mM Mg2+, in 50 mM HEPES-NaOH buffer (pH 7.2), at 37°C


organism: Corynebacterium glutamicum


organism: Escherichia coli


proof_lines: wild type enzyme, and mutant enzyme Arg703Gly/Arg704Gly


organism: Flaveria pringlei


organism: Flaveria trinervia


organism: Nostoc sp. PCC 7120 = FACHB-418


organism: Oryza sativa Japonica Group

proof_lines: K119EA mutant, presence of glucose 6-phosphate, S0.5 value, Hill coefficient 2.00, pH 7.3, 30°C

proof_lines: N-terminal replacement mutant, S0.5 value, Hill coefficient 1.77, pH 7.3, 30°C


proof_lines: wild-type, S0.5 value, Hill coefficient 1.52, pH 7.3, 30°C

proof_lines: wild-type, S0.5 value, Hill coefficient 1.63, pH 8.0, 30°C


organism: Pyrococcus furiosus

proof_lines: 50°C, pH not specified in the publication


organism: Ricinus communis

assay_conditions_summary: pH 8

proof_lines:
at pH 8.0
at pH 7.3


organism: Saccharolobus solfataricus


organism: Selenicereus undatus


organism: Sorghum bicolor

proof_lines: DELTAC4, deletion of the last 4 c-terminal amino acids


proof_lines: DELTAC1, deletion of the last c-terminal amino acid


organism: Synechocystis sp. PCC 6803


proof_lines:
presence of K+, pH 8.0, 28°C
presence of Na+, pH 8.0, 28°C


organism: Thermostichus vulcanus

substrate_name: Unknown

substrate: Unknown


organism: Zea mays


organism: Rattus norvegicus

proof_lines: mutant enzyme A467G, in 50 mM HEPES pH 7.5, 10 mM dithiothreitol, 4 mM MgCl2, at 25°C


substrate_name: GTP

proof_lines: wild type enzyme, in 50 mM HEPES pH 7.5, 10 mM dithiothreitol, 4 mM MgCl2, at 25°C

substrate: GTP


organism: Thermococcus kodakarensis


organism: Hevea brasiliensis

proof_lines: mutant enzyme F210I, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme F210I


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121M


proof_lines: mutant enzyme V106F, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme V106F


proof_lines: mutant enzyme L121Y/F125T/L146M, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T/L146M


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121H


proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme L121I


proof_lines: mutant enzyme C81L, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme C81L


proof_lines: mutant enzyme V106F/L121Y, pH and temperature not specified in the publication

proof_lines: pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y


substrate_name: acetone cyanohydrin

proof_lines:
pH 5.4
isoform HNL, at pH 7.2 and 25°C

substrate: acetone cyanohydrin


organism: Manihot esculenta

substrate_name: (S)-mandelonitrile

substrate: (S)-mandelonitrile


assay_conditions_summary: pH 5; 296.15 K; buffer pH above 6

proof_lines: wild type enzyme, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication

proof_lines: wild type enzyme, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication


substrate_name: benzaldehyde

assay_conditions_summary: pH 5; buffer pH 5

proof_lines: mutant enzyme K176P/K199P/K224P, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication

proof_lines: mutant enzyme K176P/K199P/K224P, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication

substrate: benzaldehyde


proof_lines: mutant enzyme H103Y, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication


assay_conditions_summary: pH 5; buffer pH above 6

proof_lines: mutant enzyme H103L, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication

proof_lines: mutant enzyme H103L, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication


proof_lines: mutant enzyme H103M, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication

proof_lines: mutant enzyme H103M, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication


proof_lines: mutant enzyme K176P, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication

proof_lines: mutant enzyme K176P, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication


substrate_name: 4-biphenyl carboxaldehyde

proof_lines:
pH 4.2, 22°C
pH 7.5
recombinant enzyme

substrate: 4-biphenyl carboxaldehyde


organism: Corynebacterium glutamicum


organism: Homo sapiens

assay_conditions_summary: pH 8; 303.15 K; cofactor CoA

proof_lines: 30°C, recombinant wildtype isozyme CPT1A

proof_lines: pH 8.0, 37°C, recombinant wild-type enzyme


organism: Unknown


enzyme_display_name: arylamine N-acetyltransferase

proof_lines: using control cholangiocarcinoma KKU-100 cells after treatment with the mixture of inflammatory cytokines for 48 h, Vmax: 135.7 pmol/min/mg. A significant decrease in Vmax is shown but without affecting Km, implying a change in the amount o

enzyme: arylamine N-acetyltransferase


organism: Arabidopsis thaliana

proof_lines: dephosphorylated isozyme PPC1, at pH 7.3


proof_lines:
pH 8.0
isoenzyme PC-I
isoenzyme PC-II


enzyme_display_name: glucose-6-phosphate isomerase

organism: Thermococcus litoralis

substrate_name: D-Glucose 6-phosphate

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

enzyme: glucose-6-phosphate isomerase

substrate: D-Glucose 6-phosphate


organism: Thermoplasma acidophilum

substrate_name: D-Fructose 6-phosphate

substrate: D-Fructose 6-phosphate


organism: Trypanosoma brucei


organism: Mycobacterium tuberculosis


organism: Oryctolagus cuniculus

substrate_name: D-Mannose 6-phosphate

substrate: D-Mannose 6-phosphate


organism: Plasmodium falciparum


organism: Pyrobaculum aerophilum


organism: Pyrococcus furiosus


organism: Rhizobium meliloti


organism: Saccharomyces cerevisiae


organism: Spinacia oleracea


organism: Aeropyrum pernix


organism: Aspergillus niger


organism: Bos taurus


organism: Entamoeba histolytica


organism: Escherichia coli


organism: Geobacillus stearothermophilus


organism: Homo sapiens


organism: Lactococcus lactis subsp. lactis


organism: Methanocaldococcus jannaschii


organism: Mus musculus


enzyme_display_name: glutathione-disulfide reductase

organism: Chlamydomonas reinhardtii

substrate_name: GSSG

assay_conditions_summary: cofactor NADPH

proof_lines:
isozmes GR-1 and GR-2
isozyme GR-2
isozyme GR-1

enzyme: glutathione-disulfide reductase

substrate: GSSG


organism: Picea rubens

proof_lines:
isozyme GR-1NH
isozyme GR-1H
isozyme GR-2NH


organism: Unknown

substrate_name: NADPH

substrate: NADPH


organism: Badhamia polycephala

substrate_name: glutathione disulfide

substrate: glutathione disulfide


organism: Cenchrus americanus

assay_conditions_summary: pH 8; cofactor NADPH

proof_lines: pH 8.0, temperature not specified in the publication


proof_lines: recombinant glutathione-trypanothione reductase-like enzyme


organism: Populus trichocarpa

assay_conditions_summary: pH 7.6; 295.15 K; cofactor NADPH


organism: Saccharomyces cerevisiae

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NADPH

proof_lines:
native Glr1
recombinant Glr1
pH 7.5, 37°C


organism: Xanthomonas campestris

substrate_name: NADH

substrate: NADH


substrate_name: trypanothione

proof_lines: pH 7.5, mutant enzyme A18E/N21W/R22N/N101D/K105D/R319A

substrate: trypanothione


enzyme_display_name: carbonyl reductase (NADPH)

organism: Caenorhabditis elegans

substrate_name: H+; NADPH; Isatin

enzyme: carbonyl reductase (NADPH)

substrate: H+; NADPH; Isatin


substrate_name: 4-Oxonon-2-enal; NADPH

substrate: 4-Oxonon-2-enal; NADPH


substrate_name: Glutathione-4-oxonon-2-enal; NADPH

substrate: Glutathione-4-oxonon-2-enal; NADPH


organism: Anaerobiospirillum succiniciproducens

substrate_name: oxaloacetate

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

substrate: oxaloacetate


proof_lines: pH 7.5, 30°C, above, D263N and H225Q mutants


organism: Ricinus communis

substrate_name: phosphoenolpyruvate

proof_lines:
mutant enzyme S425A, at pH 8.0 and 25°C
mutant enzyme S425A, at pH 7.3 and 25°C

substrate: phosphoenolpyruvate


proof_lines:
mutant enzyme S451D, at pH 7.3 and 25°C
mutant enzyme S451D, at pH 8.0 and 25°C


proof_lines: mutant enzyme S425A/S451D, at pH 7.3 and 25°C

proof_lines: mutant enzyme S425A/S451D, at pH 8.0 and 25°C


proof_lines:
wild type enzyme, at pH 7.3 and 25°C
wild type enzyme, at pH 8.0 and 25°C


organism: Vibrio harveyi

proof_lines:
mutant W275G, 37°C
pH 5.0, 30°C, mutant enzyme W275G


substrate_name: colloidal chitin

proof_lines: pH 5.5, 37°C, colloidal chitin, wild-type enzyme

substrate: colloidal chitin


proof_lines:
mutant W397F, 37°C
pH 5.0, 30°C, mutant enzyme W397F


organism: Ascaris suum

substrate_name: GDP

substrate: GDP


substrate_name: GTP

substrate: GTP


substrate_name: 2'-deoxyguanosine 5'-diphosphate

substrate: 2'-deoxyguanosine 5'-diphosphate


substrate_name: 2'-deoxyguanosine 5'-triphosphate

substrate: 2'-deoxyguanosine 5'-triphosphate


organism: Vibrio parahaemolyticus


mutation_signature: C-terminal truncated G589 mutant enzyme VpChiAG589

mutation_type: truncation


proof_lines: pH 7, 37°C, C-terminal truncated G589 mutant enzyme VpChiAG589


organism: Escherichia coli

substrate_name: Unknown

substrate: Unknown


organism: Corynebacterium glutamicum

proof_lines:
wild-type, pH 7.5, 30Â°C
wild type enzyme, at pH 8.5 and 25°C


organism: Arabidopsis thaliana

verification_status: unverified

proof_lines: recombinant wild-type enzyme, pH and temperature not specified in the publication

status: unverified


proof_lines: recombinant mutant Q272E, pH and temperature not specified in the publication


enzyme_display_name: firefly luciferase

organism: Photinus pyralis

substrate_name: ATP

assay_conditions_summary: pH 8.5; 298.15 K; cofactor ATP

enzyme: firefly luciferase

substrate: ATP


substrate_name: D-luciferin

assay_conditions_summary: pH 7; 298.15 K; cofactor ATP

substrate: D-luciferin


proof_lines: pH 7.5, mutant enzyme A18E/N21W/R22N/N101D/K105D


organism: Schistosoma mansoni

substrate_name: GSSG

proof_lines: truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 150 mM potassium phosphate pH 7.0, at 37°C

proof_lines: truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 100 mM potassium phosphate pH 7.0, at 25°C

substrate: GSSG


substrate_name: 5,5'-dithiobis(2-nitrobenzoic acid)

proof_lines: full length enzyme, in 100 mM potassium phosphate pH 7.0, at 25°C

proof_lines: full length enzyme, in 150 mM potassium phosphate pH 7.0, at 37°C

substrate: 5,5'-dithiobis(2-nitrobenzoic acid)


enzyme_display_name: thymidylate synthase

enzyme: thymidylate synthase


substrate_name: methylenetetrahydrofolate

substrate: methylenetetrahydrofolate


organism: Homo sapiens

assay_conditions_summary: pH 7.4; 298.15 K; buffer 50 mM TES

proof_lines: mutant enzyme E30W, in 50 mM TES buffer, 50 mM, MgCl2, 13 mM formalin, 2 mM EDTA, 0.15 mM 2-mercaptoethanol, pH 7.4, at 25°C


proof_lines: wild type enzyme, in 50 mM TES buffer, 50 mM, MgCl2, 13 mM formalin, 2 mM EDTA, 0.15 mM 2-mercaptoethanol, pH 7.4, at 25°C

proof_lines: apparent value, wild type enzyme, pH not specified in the publication, at 37°C

proof_lines: wild type enzyme, in 50 mM Tris-HCl, pH 7.4, 1 mM EDTA, 0.2% 2-mercaptoethanol, at 22°C


enzyme_display_name: fructose-bisphosphate aldolase

organism: Anoxybacillus gonensis

substrate_name: D-Fructose 1,6-bisphosphate

enzyme: fructose-bisphosphate aldolase

substrate: D-Fructose 1,6-bisphosphate


organism: Cyanophora paradoxa


organism: Drosophila melanogaster


organism: Edwardsiella ictaluri


organism: Euglena gracilis


organism: Giardia lamblia


organism: Lactococcus lactis subsp. cremoris


organism: Lampetra japonica


organism: Leishmania mexicana


organism: Magnaporthe grisea 70-15


organism: Mus musculus


organism: Mycobacterium tuberculosis


organism: Oryctolagus cuniculus


organism: Pisum sativum


organism: Pyrococcus furiosus


organism: Rattus norvegicus

substrate_name: D-Fructose 1-phosphate

substrate: D-Fructose 1-phosphate


organism: Saccharomyces cerevisiae


organism: Arabidopsis thaliana


organism: Bacillus methanolicus


organism: Bacillus subtilis


organism: Entamoeba histolytica

substrate_name: D-Glyceraldehyde 3-phosphate; Glycerone phosphate


organism: Escherichia coli


organism: Halothece sp. PCC 7418


organism: Methanocaldococcus jannaschii


organism: Plasmodium falciparum


organism: Spinacia oleracea


substrate_name: nicotinamide ribonucleotide

substrate: nicotinamide ribonucleotide


organism: Francisella tularensis

substrate_name: ADP-ribose

assay_conditions_summary: pH 8.2; 310.15 K; cofactor ADP

substrate: ADP-ribose


organism: Giardia duodenalis

assay_conditions_summary: pH 7.5; 299.15 K; cofactor ATP

proof_lines: pH 7.5, 26°C, recombinant isozyme GlNMNAT-A


organism: Homo sapiens

substrate_name: NMNH

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

proof_lines:
pH 7.5, 37°C, isozyme NMNAT2
pH 7.5, 37°C, isozyme NMNAT3
pH 7.5, 37°C, isozyme NMNAT1

substrate: NMNH


assay_conditions_summary: pH 7.5; 315.15 K; cofactor ATP


organism: Leishmania braziliensis

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

proof_lines: pH 7.4, 37°C, recombinant enzyme, kinetics following Eadie-Hofstee method

proof_lines: pH 7.4, 37°C, recombinant enzyme, kinetics following Hanes-Woolf method

proof_lines: pH 7.4, 37°C, recombinant enzyme, kinetics following Lineweaver-Burk method


organism: Mus musculus

proof_lines: isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C


proof_lines: isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C


proof_lines: isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C


assay_conditions_summary: cofactor ATP

proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant C41A/C43A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant C43A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant C41A, pH not specified in the publication, temperature not specified in the publication


organism: Sulfolobus solfataricus

substrate_name: Dihydroxyacetone phosphate; D-Glyceraldehyde 3-phosphate


organism: Synechocystis sp.


organism: Thermococcus kodakaraensis


organism: Thermoproteus tenax


organism: Thermus aquaticus


organism: Triticum aestivum


organism: Trypanosoma brucei


organism: Zea mays


enzyme_display_name: thymidine kinase

organism: Dictyostelium discoideum

substrate_name: ATP; Thymidine

enzyme: thymidine kinase

substrate: ATP; Thymidine


organism: Epstein-Barr virus


organism: Human herpesvirus 1


substrate_name: ATP; 1-beta-D-Arabinofuranosylthymine

substrate: ATP; 1-beta-D-Arabinofuranosylthymine


organism: Rattus norvegicus


organism: Staphylococcus aureus


organism: Escherichia coli


organism: Homo sapiens

substrate_name: nicotinic acid mononucleotide

assay_conditions_summary: cofactor NAD+

substrate: nicotinic acid mononucleotide


proof_lines: product inhibition of NMNAT3, substrate NMN


organism: Saccharomyces cerevisiae

substrate_name: diphosphate

substrate: diphosphate


organism: Thermus thermophilus

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP


organism: Unknown


organism: Methanothermobacter thermautotrophicus

assay_conditions_summary: pH 7.5; 338.15 K; cofactor ATP


substrate_name: NMN

substrate: NMN


enzyme_display_name: aralkylamine N-acetyltransferase

organism: Ovis aries

substrate_name: tryptamine

proof_lines: pH 6.8, 30°C, mutant enzyme S205A, phosphorylated T31

proof_lines: pH 6.8, 30°C, mutant enzyme S205A, unphosphorylated

enzyme: aralkylamine N-acetyltransferase

substrate: tryptamine


proof_lines: pH 6.8, 30°C, mutant enzyme T31A, phosphorylated S205

proof_lines: pH 6.8, 30°C, mutant enzyme T31A, unphosphorylated


enzyme_display_name: corrinoid adenosyltransferase

substrate_name: cob(I)alamin

proof_lines: pH 8.0, 37°C, recombinant GST-tagged mutant R191W

enzyme: corrinoid adenosyltransferase

substrate: cob(I)alamin


proof_lines: pH 8.0, 37°C, recombinant wild-type GST-tagged enzyme


enzyme_display_name: cysteine synthase

substrate_name: O-acetyl-L-serine

enzyme: cysteine synthase

substrate: O-acetyl-L-serine


proof_lines:
wild-type, 37°C
wild-type


organism: Thermotoga maritima

substrate_name: ATP; Deoxythymidine

substrate: ATP; Deoxythymidine


organism: Vaccinia virus

substrate_name: Deoxythymidine; ATP

substrate: Deoxythymidine; ATP


enzyme_display_name: pyroglutamyl-peptidase II

substrate_name: Thyroliberin

enzyme: pyroglutamyl-peptidase II

substrate: Thyroliberin


enzyme_display_name: coagulation factor XIIa

proof_lines: recombinant His-tagged betaFXIIa, pH and temperature not specified in the publication

proof_lines: recombinant MBP-tagged betaFXIIa, pH and temperature not specified in the publication

proof_lines: alphaFXIIa, pH and temperature not specified in the publication

enzyme: coagulation factor XIIa


organism: Salmonella enterica subsp. enterica serovar Typhimurium

proof_lines: turnover for mutant K120Q is decreased 56fold compared to wild type, 25°C, 100 mM HEPES, pH 7.0

proof_lines: the turnover for H152A mutant is decreased 9fold compared to the wild type, 25°C, 100 mM MES, pH 6.5


organism: Mus musculus

assay_conditions_summary: pH 7.5; cofactor ATP

proof_lines: wild type enzyme, in 50 mM HEPES-NaOH, pH 7.5, temperature not specified in the publication


proof_lines: mutant enzyme C164S/C165S, in 50 mM HEPES-NaOH, pH 7.5, temperature not specified in the publication


substrate_name: UTP

proof_lines: recombinant wild-type enzyme, pH 8.0, 25°C

substrate: UTP


substrate_name: Unknown

substrate: Unknown


substrate_name: 4-nitrophenyl phosphate

assay_conditions_summary: pH 7; 295.15 K; cofactor ATP

substrate: 4-nitrophenyl phosphate


enzyme_display_name: chitinase

organism: Aeromonas caviae

proof_lines: pH 6.0, 50°C, recombinant His-tagged wild-type enzyme

enzyme: chitinase


proof_lines: pH 6.0, 50°C, recombinant His-tagged truncation mutant enzyme


organism: Manduca sexta


proof_lines:
37°C, pH 6, Chi386(MCBD)2
37°C, pH 6, Chi386
37°C, pH 6, Chi386(MCBD)


organism: Trichoderma viride


enzyme_display_name: scytalidoglutamic peptidase

organism: Scytalidium lignicola

proof_lines: pH and temperature not specified in the publication

enzyme: scytalidoglutamic peptidase


enzyme_display_name: pyrimidine-nucleoside phosphorylase

organism: Streptococcus pyogenes

substrate_name: uridine

proof_lines: mutant H169A, pH 7.5, temperature not specified in the publication

enzyme: pyrimidine-nucleoside phosphorylase

substrate: uridine


proof_lines: wild-type, pH 7.5, temperature not specified in the publication


source_db: skid

source_license: CC BY-NC-ND 4.0

source: skid


substrate_name: thymidine

substrate: thymidine


enzyme_display_name: polo kinase

organism: Danio rerio

substrate_name: ATP

proof_lines:
zPlk1 1-312 T196D
apparent Km, zPlk1 1-312 T196D

enzyme: polo kinase

substrate: ATP


proof_lines:
zPlk1 1-312 wild type
apparent Km, zPlk1 1-312 wild type


source_license: CC BY 4.0; CC BY-NC-ND 4.0

proof_lines:
time-dependent irreversible inhibition
Raw SKiD Env: Temp=-----, pH=-----

source_databases_merged: brenda skid


proof_lines:
time-dependent inhibition
Raw SKiD Env: Temp=-----, pH=-----


proof_lines: wild-type enzyme, varied substrate Cdc25c, constant substrate ATP

proof_lines: apparent Km, wild-type enzyme, varied substrate ATP, constant substrate Cdc25c


proof_lines: T210V mutant, varied substrate Cdc25c, constant substrate ATP

proof_lines: apparent Km, T210V mutant, varied substrate ATP, constant substrate Cdc25c


proof_lines: T210D mutant, varied substrate Cdc25c, constant substrate ATP

proof_lines: T210D mutant, varied substrate ATP, constant substrate Cdc25c


organism: Vibrio harveyi

substrate_name: chitohexaose

proof_lines: pH 5.5, 37°C, colloidal chitin, mutant enzym S33A

substrate: chitohexaose


proof_lines: pH 5.5, 37°C, colloidal chitin, mutant enzym Y245W


organism: Homo sapiens

proof_lines: mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C


proof_lines: wild type enzyme alphaFXIIa, at pH 7.4 and 37°C


enzyme_display_name: subtilisin

organism: Bacillus amyloliquefaciens

enzyme: subtilisin


proof_lines:
pH 7.4, 37°C, wild-type
wild-type


substrate_name: succinyl-Ala-Ala-Pro-Phe 4-nitroanilide

substrate: succinyl-Ala-Ala-Pro-Phe 4-nitroanilide


organism: Lederbergia lenta

substrate_name: succinyl-AAPR-p-nitroanilide

proof_lines:
pH 8.6, 25°C mutant S166C-S-d
pH 8.6, 25°C mutant S166C-S-j
pH 8.6, 25°C mutant S166C-S-g

substrate: succinyl-AAPR-p-nitroanilide


substrate_name: succinyl-AAPA-p-nitroanilide

proof_lines: pH 8.6, 25°C mutant S166C-S-e and S166S-f

substrate: succinyl-AAPA-p-nitroanilide


enzyme_display_name: limit dextrinase

organism: Hordeum vulgare

substrate_name: pullulan

proof_lines: fitting the uncompetitive substrate inhibition Michaelis-Menten equation, pH 5.5, 40°C

proof_lines: classical Michaelis-Menten kinetics, pH 5.5, 40°C

enzyme: limit dextrinase

substrate: pullulan


proof_lines: wild-type, 37°C, pH not specified in the publication


substrate_name: amylopectin

proof_lines: mutant M440G, 37°C, pH not specified in the publication

substrate: amylopectin


organism: Hordeum vulgare subsp. vulgare

substrate_name: beta-limit dextrin

substrate: beta-limit dextrin


substrate_name: L-Tyr-L-Val-L-Ala-L-Asp

proof_lines:
wild-type, pH 9.0, 24°C
pH 8.6, 25°C wild-type
pH 7.5, 25°C, wild-type

substrate: L-Tyr-L-Val-L-Ala-L-Asp


substrate_name: L-Ala-L-Ala-L-Val-L-Ala

substrate: L-Ala-L-Ala-L-Val-L-Ala


enzyme_display_name: N-acyl-D-amino-acid deacylase

organism: Achromobacter xylosoxidans

substrate_name: N-Acetyl-D-Trp

proof_lines: mutant enzyme L298A, in 100 mM HEPES (pH 7.0), at 30°C

enzyme: N-acyl-D-amino-acid deacylase

substrate: N-Acetyl-D-Trp


substrate_name: N-Acetyl-D-Ala

proof_lines: wild type enzyme, in 100 mM HEPES (pH 7.0), at 30°C

substrate: N-Acetyl-D-Ala


proof_lines: mutant enzyme F191W, in 100 mM HEPES (pH 7.0), at 30°C


organism: Alcaligenes faecalis

substrate_name: N-Acetyl-D-Met

substrate: N-Acetyl-D-Met


organism: Microbacterium natoriense

substrate_name: N-acetyl-D-phenylalanine

substrate: N-acetyl-D-phenylalanine


organism: Variovorax paradoxus

substrate_name: N-acetyl-D-methionine

proof_lines: recombinant mutant M254L, pH and temperature not specified in the publication

substrate: N-acetyl-D-methionine


proof_lines: recombinant mutant M39L, pH and temperature not specified in the publication


proof_lines: recombinant mutant M56L, pH and temperature not specified in the publication


proof_lines: recombinant mutant M221L, pH and temperature not specified in the publication


proof_lines: recombinant mutant M352L, pH and temperature not specified in the publication


proof_lines: recombinant wild-type enzyme, pH and temperature not specified in the publication


substrate_name: N-acetyl-D-leucine

substrate: N-acetyl-D-leucine


enzyme_display_name: camphor 5-monooxygenase

organism: Pseudomonas putida

substrate_name: (+)-Camphor

enzyme: camphor 5-monooxygenase

substrate: (+)-Camphor


substrate_name: NADH

assay_conditions_summary: pH 7.4; 295.15 K; cofactor NADH

proof_lines: pH 7.4, 22°C, recombinant mutant H352A enzyme with mutant S42A putidaredoxin

substrate: NADH


proof_lines: pH 7.4, 22°C, recombinant mutant D125A enzyme with mutant Y33A putidaredoxin


proof_lines: mutant Y179H, pH 7.4, temperature not specified in the publication


proof_lines: mutant G60S/Y75H, pH 7.4, temperature not specified in the publication


proof_lines: mutant E156G/V247F/V253G/F256S, pH 7.4, temperature not specified in the publication


proof_lines: mutant G93C/K314R/L319M, pH 7.4, temperature not specified in the publication


proof_lines: mutant D97F/P122L/Q183L/L244Q, pH 7.4, temperature not specified in the publication


proof_lines: mutant G120A/Y179H/G248S/D297H, pH 7.4, temperature not specified in the publication


proof_lines: mutant T56A/N116H/D297N, pH 7.4, temperature not specified in the publication


substrate_name: reduced putidaredoxin

assay_conditions_summary: pH 7.4; 303.15 K; cofactor NADH

proof_lines: putidaredoxin reductase-camphor monooxygenase fusion enzyme, pH 7.4, 30°C

proof_lines: pH and temperature not specified in the publication, with P450tcu putidaredoxin

proof_lines: pH and temperature not specified in the publication, with P450 putidaredoxin

substrate: reduced putidaredoxin


proof_lines: pH 7.4, 22°C, recombinant mutant D125A enzyme with wild-type putidaredoxin

proof_lines: pH 7.4, 22°C, recombinant wild-type enzyme with mutant S44Aputidaredoxin


enzyme_display_name: tyramine N-feruloyltransferase

organism: Capsicum annuum

substrate_name: tyramine

enzyme: tyramine N-feruloyltransferase

substrate: tyramine


assay_conditions_summary: pH 8.5; 303.15 K; cofactor CoA


organism: Lavandula angustifolia

assay_conditions_summary: pH 7.5; 303.15 K; cofactor CoA

proof_lines: with tyramine, pH 7.5, 30°C, recombinant AAT1

proof_lines: with phenethylamine, pH 7.5, 30°C, recombinant AAT1

proof_lines: with caffeoyl-CoA, pH 7.5, 30°C, recombinant AAT1


enzyme_display_name: N-acetylgalactosamine kinase

substrate_name: N-acetyl-alpha-D-galactosamine

proof_lines:
pH 8.0, 37°C
Raw SKiD Env: Temp=37°C, pH=7.5
Raw SKiD Env: Temp=37°C, pH=8

enzyme: N-acetylgalactosamine kinase

substrate: N-acetyl-alpha-D-galactosamine


proof_lines: mutant F444C, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine

proof_lines: mutant F444C, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine


proof_lines: mutant F444W, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine


substrate_name: N-acetyl-alpha-D-mannosamine

proof_lines: mutant F444R, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine

proof_lines: mutant F444R, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine

substrate: N-acetyl-alpha-D-mannosamine


proof_lines: wild-type, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine

proof_lines: wild-type, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine


proof_lines: mutant F444H, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine

proof_lines: mutant F444H, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine


proof_lines: mutant F444K, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine

proof_lines: mutant F444K, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine


proof_lines: mutant F444S, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine

proof_lines: mutant F444S, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine


substrate_name: N-acetyl-alpha-D-glucosamine

proof_lines: mutant F444A, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine

proof_lines: mutant F444A, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine

substrate: N-acetyl-alpha-D-glucosamine


organism: uncultured bacterium

proof_lines: pH and temperature not specified in the publication


organism: Elizabethkingia meningoseptica

substrate_name: 4-nitrophenyl alpha-L-fucoside

substrate: 4-nitrophenyl alpha-L-fucoside


substrate_name: Lewis X trisaccharide

substrate: Lewis X trisaccharide


proof_lines: mutant enzyme 249DELTA265, at pH 4.6 and 37°C


enzyme_display_name: penicillin amidase

organism: Achromobacter sp.

substrate_name: methyl D-(-)-4-hydroxy-phenyl glycinate

enzyme: penicillin amidase

substrate: methyl D-(-)-4-hydroxy-phenyl glycinate


organism: Escherichia coli

substrate_name: D-phenylglycine amide

proof_lines:
wild-type enzyme
pH 7.0, 25°C, wild-type enzyme
wild-type, pH 7.0, 30°C

substrate: D-phenylglycine amide


proof_lines:
mutant alphaR145G
mutant alphaR145S
mutant alphaR145L


organism: Mesocricetus auratus

proof_lines: pH 7.4, 25°C, with substrate S-camphorquinone


organism: Rattus norvegicus


enzyme_display_name: alanine racemase

organism: Bacillus subtilis

substrate_name: L-alanine

enzyme: alanine racemase

substrate: L-alanine


organism: Geobacillus stearothermophilus

substrate_name: D-alanine

proof_lines: pH 8.0, 30°C, recombinant wild-type enzyme

substrate: D-alanine


substrate_name: D-Lysine

proof_lines: pH 8.0, 30°C, recombinant mutant I222T/Y354W

substrate: D-Lysine


organism: Helicobacter pylori

proof_lines:
Vmax 909 micromol/min/mg
Vmax 37.9 micromol/min/mg


organism: Streptomyces lavendulae


proof_lines: +/-0.250, Alr D164K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.033, Alr D164K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.070, Alr D164K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


proof_lines: +/-0.200, Alr D164A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-2.600, Alr D164A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.114, Alr D164A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


proof_lines: +/-0.333, Alr E221K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-7.550, Alr E221K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.055, Alr E221K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


proof_lines: +/-0.100, Alr E165K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.850, Alr E165K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.079, Alr E165K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


proof_lines: +/-0.417, Alr E165A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-2.667, Alr E165A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.085, Alr E165A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


proof_lines: +/-0.483, Alr wildtype, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-3.217, Alr wildtype, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.008, Alr wildtype, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


organism: Caldanaerobacter subterraneus subsp. tengcongensis


proof_lines: pH 10.0, 65°C, recombinant wild-type enzyme


proof_lines: +/-0.333, Alr P219A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-6.067, Alr P219A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.034, Alr P219A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


proof_lines: +/-0.750, Alr E221P, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-7.633, Alr E221P, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.084, Alr E221P, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


proof_lines: +/-0.650, Alr E221A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-2.333, Alr E221A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm

proof_lines: +/-0.080, Alr E221A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm


proof_lines: pH 10.0, 65°C, recombinant mutant S173D/Q360Y


organism: Clostridioides difficile

proof_lines: pH 8.5, temperature not specified in the publication, recombinant wild-type enzyme

proof_lines: pH 7.5, 37°C, recombinant wild-type enzyme


proof_lines: pH 8.5, temperature not specified in the publication, recombinant mutant K271T


organism: Candidatus Pelagibacter communis

proof_lines: recombinant MBP-tagged enzyme, pH 8.0, 37°C


organism: Mycobacterium tuberculosis


organism: Synechocystis sp. PCC 6803


organism: Thermotoga maritima

assay_conditions_summary: pH 8; 310.15 K; buffer recombinant His-tagged enzyme

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C, Tris buffer

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C, bicine buffer

proof_lines: recombinant His-tagged enzyme, pH 8.0, 70°C, bicine buffer


organism: Wolbachia pipientis

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C


organism: Clostridium perfringens


enzyme_display_name: hepsin

organism: Homo sapiens

substrate_name: Unknown

assay_conditions_summary: pH 8

proof_lines: at pH 8.0, temperature not specified in the publication

proof_lines: pH not specified in the publication, temperature not specified in the publication

enzyme: hepsin

substrate: Unknown


substrate_name: D-Ala

substrate: D-Ala


enzyme_display_name: HIV-2 retropepsin

organism: Human immunodeficiency virus 2

enzyme: HIV-2 retropepsin


enzyme_display_name: orotidine-5'-phosphate decarboxylase

organism: Saccharomyces cerevisiae

substrate_name: orotidine 5'-phosphate

proof_lines:
Q215A mutant
mutant Q215A, pH 7.1, 25°C

enzyme: orotidine-5'-phosphate decarboxylase

substrate: orotidine 5'-phosphate


proof_lines: wild-type, pH 7.1, 25°C, presence of 0.1 M NaCl


enzyme_display_name: phosphoenolpyruvate carboxylase

organism: Echinochloa esculenta

proof_lines:
wild-type, pH 7.6, 25°C
wild-type, Hill value 1.1, pH 7.6, 25°C

enzyme: phosphoenolpyruvate carboxylase


proof_lines:
hybrid mutant V674-880, pH 7.6, 25°C
hybrid mutant V528-880, pH 7.6, 25°C
hybrid mutant V528-673, pH 7.6, 25°C


organism: Oryza sativa Japonica Group

substrate_name: phosphoenolpyruvate

proof_lines: mutant S12D, presence of glucose 6-phosphate, S0.5 value, Hill coeffcient 1.64, pH 7.3, 30°C

proof_lines: mutant S12D, S0.5 value, Hill coeffcient 1.10, pH 7.3, 30°C

substrate: phosphoenolpyruvate


proof_lines: wild-type, S0.5 value, Hill coeffcient 2.06, pH 7.3, 30°C

proof_lines: wild-type, presence of glucose 6-phosphate, S0.5 value, Hill coeffcient 1.84, pH 7.3, 30°C


enzyme_display_name: phosphoenolpyruvate carboxykinase (GTP)

organism: Sus scrofa

proof_lines: mutant M139L, substrate oxaloacetate, pH 7.4, temperature not specified in the publication

proof_lines: mutant M139L, substrate phosphoenolpyruvate, pH 7.4, temperature not specified in the publication

proof_lines: mutant M139L, substrate GDP, pH 7.4, temperature not specified in the publication

enzyme: phosphoenolpyruvate carboxykinase (GTP)


proof_lines: wild-type, substrate oxaloacetate, pH 7.4, temperature not specified in the publication

proof_lines: wild-type, substrate GDP, pH 7.4, temperature not specified in the publication

proof_lines: wild-type, substrate phosphoenolpyruvate, pH 7.4, temperature not specified in the publication


enzyme_display_name: (S)-hydroxynitrile lyase

organism: Manihot esculenta

substrate_name: acetone cyanohydrin

enzyme: (S)-hydroxynitrile lyase

substrate: acetone cyanohydrin


proof_lines:
wild-type enzyme
pH 5.4, 25°C, wild-type enzyme


enzyme_display_name: 2-dehydro-3-deoxy-L-fuconate aldolase

organism: Veillonella ratti

enzyme: 2-dehydro-3-deoxy-L-fuconate aldolase


enzyme_display_name: tryptophan synthase

substrate_name: indole

enzyme: tryptophan synthase

substrate: indole


substrate_name: L-serine

substrate: L-serine


organism: Salmonella enterica subsp. enterica serovar Typhimurium

proof_lines: overall-reaction, pH 7.8, 25°C, wild-type enzyme

proof_lines: beta-reaction, pH 7.8, 25°C, wild-type enzyme

proof_lines: alpha-reaction, wild-type enzyme, pH 7.8, 25°C


proof_lines: beta-reaction, pH 7.8, 25°C, mutant T183V enzyme

proof_lines: overall-reaction, pH 7.8, 25°C, mutant T183V enzyme

proof_lines: alpha-reaction, pH 7.8, 25°C, mutant T183V enzyme


substrate_name: L-threonine

proof_lines: mutant L166V, pH not specified in the publication, temperature not specified in the publication

substrate: L-threonine


proof_lines: mutant L166A, pH not specified in the publication, temperature not specified in the publication


enzyme_display_name: 4-hydroxyphenylpyruvate dioxygenase

organism: Echinochloa crus-galli

enzyme: 4-hydroxyphenylpyruvate dioxygenase


organism: Avena sativa


organism: Arabidopsis thaliana

proof_lines: activity is measured polarographically, assays include 0.5 microM of enzyme, 500 microM betaME, Fe2+ stoichiometric with enzyme, and 400 microM 4-hydroxyophenylpyruvate in 20 mM HEPES at pH 7.0 at 4°C


proof_lines:
enzyme form 1
enzyme form 3
enzyme form 2


organism: Streptomyces avermitilis

proof_lines: Solvent is H2O. Steady-state analyses of 4-hydroxyphenylpyruvate dioxygenase using proteo-HPP, 2,3,5,6-tetradeutero-HPP, and proteo-HPP in deuterium oxide are carried out in 1 ml assays on a Hasetech oxygen electrode. The assay mixture incl

proof_lines: Solvent is D2O. Steady-state analyses of 4-hydroxyphenylpyruvate dioxygenase using proteo-HPP, 2,3,5,6-tetradeutero-HPP, and proteo-HPP in deuterium oxide are carried out in 1 ml assays on a Hasetech oxygen electrode. The assay mixture incl


proof_lines: pH and temperature not specified in the publication


enzyme_display_name: firefly luciferase

organism: Photinus pyralis

substrate_name: D-luciferin

assay_conditions_summary: pH 8.2; buffer phosphate buffer; cofactor ATP

proof_lines: pH 8.2, temperature not specified in the publication

proof_lines: pH 8.5, temperature not specified in the publication

proof_lines: pH 9.1, temperature not specified in the publication

enzyme: firefly luciferase

substrate: D-luciferin


organism: Zea mays


organism: Luciola mingrelica

substrate_name: Luciferin

proof_lines: 150 microM luciferin, presence of 1 microM of N-quinolin-2-ylbenzamide, two-site binding model

substrate: Luciferin


organism: Pseudomonas putida


proof_lines: P-450cam, constituent part of the multi-component enzyme

proof_lines: constituent protein of the multi-component oxygenase


organism: Pseudomonas sp. TCU-HL1

substrate_name: reduced putidaredoxin

proof_lines: redox partner is Pdx, i.e native redox partner for isoform P450cam, pH 7.4, 25°C

proof_lines: redox partner is Pdxtcu, i.e native redox partner for isoform P450tcu, pH 7.4, 25°C

substrate: reduced putidaredoxin


organism: Homo sapiens

substrate_name: testosterone

assay_conditions_summary: pH 7.4; 310.15 K; cofactor NAD+

proof_lines:
pH 7.4, with NAD+ as cofactor
pH 7.4, reaction with estradiol
pH 7.4, reaction with testosterone

substrate: testosterone


proof_lines:
microsomal enzyme
cytosolic enzyme
mitochondrial enzyme


enzyme_display_name: glutathione-disulfide reductase

organism: Allochromatium vinosum

substrate_name: NADH

assay_conditions_summary: cofactor NADPH

enzyme: glutathione-disulfide reductase

substrate: NADH


organism: Anabaena sp.

substrate_name: NADPH

substrate: NADPH


assay_conditions_summary: pH 7.4; buffer 100 mM K2HPO4/KH2PO4; cofactor NADPH

proof_lines: in 100 mM K2HPO4/KH2PO4 buffer, at pH 7.4


organism: Bos taurus

assay_conditions_summary: pH 7; 303.15 K; cofactor NADPH

proof_lines:
calf liver enzyme, pH 7.0, 30°C
pH 7.4, 37°C
erythrocyte enzyme, pH 7.0, 30°C


organism: Camelus dromedarius

substrate_name: glutathione disulfide

assay_conditions_summary: pH 7.5; 323.15 K; cofactor NADPH

substrate: glutathione disulfide


organism: Colwellia psychrerythraea

assay_conditions_summary: 298.15 K; cofactor NADPH

proof_lines: 25°C, pH not specified in the publication

proof_lines: 4°C, pH not specified in the publication


organism: Cyberlindnera mrakii


organism: Acinetobacter baumannii

substrate_name: D-alanine

proof_lines: pH not specified in the publication, 30°C

substrate: D-alanine


organism: Aeromonas hydrophila subsp. hydrophila

proof_lines: pH 11.0, 35°C, recombinant His-tagged enzyme


substrate_name: L-alanine

proof_lines: recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication

substrate: L-alanine


organism: Alkalihalophilus pseudofirmus


proof_lines: pH 10.5, 40°C, recombinant wild-type enzyme


organism: Bacillus anthracis


organism: Caldanaerobacter subterraneus subsp. tengcongensis


organism: Clostridioides difficile


organism: Geobacillus stearothermophilus

substrate_name: Unknown

substrate: Unknown


organism: Peribacillus psychrosaccharolyticus

substrate_name: D-Ala

substrate: D-Ala


organism: Pseudomonas aeruginosa

proof_lines: pH and temperature not specified in the publication


enzyme_display_name: arylacetonitrilase

organism: Alcaligenes sp.

substrate_name: mandelonitrile

proof_lines:
at pH 6.5 and 50°C
at pH 6.5 and 30°C

enzyme: arylacetonitrilase

substrate: mandelonitrile


organism: Aspergillus niger

substrate_name: phenylpropionitrile

substrate: phenylpropionitrile


organism: uncultured bacterium

substrate_name: phenylacetonitrile

substrate: phenylacetonitrile


enzyme_display_name: inorganic diphosphatase

organism: Anabaena sp.

substrate_name: Diphosphate; H2O

enzyme: inorganic diphosphatase

substrate: Diphosphate; H2O


organism: Arabidopsis thaliana

substrate_name: H2O; Diphosphate

substrate: H2O; Diphosphate


organism: Synechocystis sp.


organism: Vigna radiata

substrate_name: Bis-Tris-propane diphosphate; H2O

substrate: Bis-Tris-propane diphosphate; H2O


organism: Pyrococcus horikoshii


organism: Salmonella enteritidis PT4 P125109


organism: Schizosaccharomyces pombe


organism: Shigella dysenteriae

substrate_name: L-Ala

substrate: L-Ala


organism: Staphylococcus aureus

proof_lines: pH not specified in the publication, 30°C, recombinant enzyme


organism: Streptococcus iniae


organism: Streptococcus pneumoniae


organism: Alcaligenes faecalis


organism: Hemicentrotus pulcherrimus


organism: Homo sapiens

substrate_name: (Z)-ajoene

assay_conditions_summary: pH 7.5; 298.15 K; buffer 100 mM Tris-HCl; cofactor NADPH

proof_lines:
25°C
pH 7.5
reoxidation

substrate: (Z)-ajoene


organism: Ipomoea batatas

assay_conditions_summary: pH 8; 298.15 K; buffer 100 mM potassium phosphate; cofactor NADPH

proof_lines: in 100 mM potassium phosphate buffer (pH 8.0), at 25°C


organism: Lathyrus oleraceus

substrate_name: GSSG

assay_conditions_summary: cofactor NADP+

proof_lines:
root enzyme
root and chloroplast enzyme
chloroplast enzyme

substrate: GSSG


organism: Limnospira maxima

substrate_name: mixed disulfide of CoA and glutathione

assay_conditions_summary: cofactor CoA

substrate: mixed disulfide of CoA and glutathione


organism: Lithobates sylvaticus

proof_lines: enzyme from unfrozen state, presence of glucose, pH 7.2, 25°C

proof_lines: enzyme from unfrozen state, presence of urea, pH 7.2, 25°C


organism: Litomosoides carinii


organism: Moniezia expansa

substrate_name: NADH

assay_conditions_summary: cofactor NADH

substrate: NADH


organism: Oncorhynchus mykiss

assay_conditions_summary: pH 8; 298.15 K; cofactor NADPH


organism: Ovis aries

substrate_name: NADPH

substrate: NADPH


organism: Phaeodactylum tricornutum

substrate_name: glutathione disulfide

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADPH

proof_lines: non-competitive product inhibition regarding NADPH, at 30°C and pH 7.5

proof_lines: competitive product inhibition regarding glutathione disulfide, at 30°C and pH 7.5

substrate: glutathione disulfide


organism: Plasmodium falciparum


proof_lines: kcat_Km above 0.8 1/sec*mM, pH 7.0, 0.1 M potassium phosphate, 1 mM EDTA, 25°Ckcat above 0.1 1/sec, pH 7.0, 0.1 M potassium phosphate, 1 mM EDTA, 25°C


organism: Psychrobacter sp. ANT206

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH


organism: Pteris ensiformis

assay_conditions_summary: pH 7; buffer 50 mM potassium phosphate; cofactor NADPH

proof_lines: in 50 mM potassium phosphate buffer (pH 7.0)


organism: Pteris vittata

assay_conditions_summary: pH 7; buffer note; cofactor NADPH


organism: Rattus norvegicus

assay_conditions_summary: pH 7.4; 298.15 K; buffer 100 mM potassium phosphate; cofactor NADPH

proof_lines: 25°C, pH not specified in the publication

proof_lines: in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C


organism: Saccharomyces cerevisiae

substrate_name: S-allylmercaptoglutathione disulfide

proof_lines: at 25°C, pH not specified in the publication

substrate: S-allylmercaptoglutathione disulfide


organism: Scombridae


organism: Spinacia oleracea


organism: Sus scrofa


organism: Taenia crassiceps

assay_conditions_summary: pH 7.8; 298.15 K; cofactor NADPH


organism: Trachemys scripta

assay_conditions_summary: pH 7.4; cofactor NADPH

proof_lines: anoxic enzyme in 50 mM potassium phosphate, pH 7.4, 1 mM EDTA, 0.1 mM GSSG and 0.2 mM NADPH


organism: Triticum aestivum

assay_conditions_summary: cofactor NADPH


organism: Triticum turgidum subsp. durum


enzyme_display_name: thymidylate synthase

organism: Angiostrongylus cantonensis

proof_lines: 0.25 mM, noncompetitive inhibition, 5,10-mehylenetetrahydrofolate as variable substrate

proof_lines: 1.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate

enzyme: thymidylate synthase


organism: Bacillus subtilis


organism: Bos taurus


organism: Enterobacteria phage T6


organism: Escherichia coli


organism: Escherichia phage T2


organism: Gallus gallus


organism: Homo sapiens

substrate_name: dUMP

proof_lines: apparent value, mutant enzyme V3A, pH not specified in the publication, at 37°C

substrate: dUMP


substrate_name: Unknown

proof_lines: mutant enzyme A191K, in 50 mM Tris-HCl, pH 7.4, 1 mM EDTA, 0.2% 2-mercaptoethanol, at 22°C

substrate: Unknown


proof_lines: uncompetitive with methylene tetrahydrofolate


organism: Lacticaseibacillus casei


organism: Kitasatospora aureofaciens


organism: Plasmodium berghei


organism: Mycobacterium tuberculosis


organism: Mus musculus

substrate_name: methylenetetrahydrofolate

proof_lines:
Ehrlich ascites carcinoma cells
L1210 recombinant enzyme
L1210 parental enzyme

substrate: methylenetetrahydrofolate


organism: Saccharomyces cerevisiae


organism: Streptococcus pneumoniae


organism: Rattus norvegicus


organism: Plasmodium falciparum

proof_lines:
recombinant enzyme
native enzyme


enzyme_display_name: tyramine N-feruloyltransferase

organism: Nicotiana tabacum

proof_lines:
+ tyramine
+ feruloyl-CoA
+ feruloyl-CoA, dopamine + feruloyl-CoA

enzyme: tyramine N-feruloyltransferase


organism: Capsicum annuum

substrate_name: feruloyl-CoA

assay_conditions_summary: pH 8.5; 303.15 K; cofactor CoA

substrate: feruloyl-CoA


organism: Zea mays

substrate_name: tyramine

proof_lines:
with feruloyl-CoA as acceptor
with tyramine as acceptor

substrate: tyramine


organism: Papaver somniferum


enzyme_display_name: carnitine O-palmitoyltransferase

organism: Bos taurus

substrate_name: L-carnitine

proof_lines:
peroxisomal soluble enzyme
with C8-CoA
inner mitochondrial membrane

enzyme: carnitine O-palmitoyltransferase

substrate: L-carnitine


organism: Solanum tuberosum


organism: Ctenopharyngodon idella

proof_lines: enzyme from 6 month old animal, at 25°C, pH not specified in the publication

proof_lines: enzyme from 1 month old animal, at 25°C, pH not specified in the publication

proof_lines: enzyme at hatching, at 25°C, pH not specified in the publication


organism: Felis catus

proof_lines:
pH 7.2, 30°C, liver CPT 1
pH 7.2, 30°C, skeletal muscle CPT 1


organism: Gallus gallus

substrate_name: butyryl-CoA

substrate: butyryl-CoA


organism: Drosophila melanogaster

substrate_name: palmitoyl-CoA

proof_lines: KI-values: 0.00064 mM and 0.0007 mM malony-CoA (from secondary Dixon plots)

substrate: palmitoyl-CoA


organism: Homo sapiens

assay_conditions_summary: 303.15 K; cofactor CoA


assay_conditions_summary: pH 8; 310.15 K; cofactor CoA

proof_lines: pH 8.0, 37°C, recombinant revertant A305C


proof_lines: D17E mutant protein, mitochondrial preparation


organism: Macrobrachium borellii


substrate_name: stearoyl-CoA

substrate: stearoyl-CoA


proof_lines:
recombinant wild-type L-CPT I
recombinant wild-type CPT I


assay_conditions_summary: pH 7.4; 303.15 K; cofactor CoA

proof_lines: isoform L-CPT I, with 2[6-(4-chlorophenoxy)hexyl]oxirane-2-carboxylic acid

proof_lines: purified recombinant L-CPT I, reconstituted in liposomes


organism: Sus scrofa

assay_conditions_summary: pH 7.2; 303.15 K; cofactor CoA

proof_lines:
3 wk old pig, liver
5 wk old pig, liver
adult pig, skeletal muscle


proof_lines: wild-type protein, mitochondrial preparation


proof_lines: E17D mutant protein, mitochondrial preparation


enzyme_display_name: arylamine N-acetyltransferase

substrate_name: Phenylethylamine

proof_lines: liver brain

enzyme: arylamine N-acetyltransferase

substrate: Phenylethylamine


organism: Bacillus cereus

proof_lines:
Vmax: 2.1 micromol/min/mg
Vmax: 0.17 micromol/min/mg


enzyme_display_name: aralkylamine N-acetyltransferase

substrate_name: acetyl-CoA

enzyme: aralkylamine N-acetyltransferase

substrate: acetyl-CoA


organism: Arabidopsis thaliana


organism: Aedes aegypti

substrate_name: dopamine

proof_lines: isoform aaNAT2, pH not specified in the publication, temperature not specified in the publication

substrate: dopamine


organism: Ovis aries

assay_conditions_summary: pH 6.8; 303.15 K; cofactor CoA

proof_lines: pH 6.8, 30°C, phosphorylated T31, unphosphorylated S205


organism: Onchocerca volvulus


substrate_name: serotonin

proof_lines:
wild-type enzyme
wild-type enzyme with 14-3-3 protein
wild-type enzyme with protein kinase A

substrate: serotonin


organism: Pyropia yezoensis


organism: Rattus norvegicus

assay_conditions_summary: cofactor CoA

proof_lines:
cosubstrate tryptamine, pineal gland
cosubstrate tryptamine, liver
pineal gland


organism: Saccharopolyspora erythraea

assay_conditions_summary: pH 7; 298.15 K; cofactor CoA

proof_lines:
cosubstrate acetyl-CoA, pH 7.0, 25°C
cosubstrate octopamine, pH 7.0, 25°C


organism: Pelophylax perezi


enzyme_display_name: pyrimidine-nucleoside phosphorylase

organism: Escherichia coli

proof_lines: pH 7.5, 25°C, thymidine phosphorylase activity

proof_lines: pH 7.5, 25°C, uridine phosphorylase activity

enzyme: pyrimidine-nucleoside phosphorylase


organism: Bacillus subtilis

proof_lines: pH and temperature not specified in the publication


organism: Geobacillus stearothermophilus

substrate_name: uridine

substrate: uridine


organism: Haemophilus influenzae


organism: Parageobacillus thermoglucosidasius

substrate_name: thymidine

proof_lines:
pH 7.0, 60°C
Km about 1 mM, at pH 7.0 and 40°C
at pH 7.0 and 40°C

substrate: thymidine


organism: Mesomycoplasma hyorhinis

substrate_name: phosphate

proof_lines:
cosubstrate uridine, pH 7.6, 37°C
at pH 7.6 and 37°C
pH 7.6, 37°C

substrate: phosphate


enzyme_display_name: corrinoid adenosyltransferase

organism: Homo sapiens

substrate_name: cob(I)alamin

assay_conditions_summary: 239.00 K; cofactor ATP

proof_lines:
recombinant enzyme variant 239K
recombinant enzyme variant 239M

enzyme: corrinoid adenosyltransferase

substrate: cob(I)alamin


organism: Limosilactobacillus reuteri

substrate_name: ATP

assay_conditions_summary: cofactor ATP

substrate: ATP


organism: Lactobacillus leichmannii

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP


organism: Clostridium tetanomorphum

substrate_name: cyanocob(I)alamin

proof_lines: pH 8.0, 37°C, i.e. vitamin B12, reduced form

substrate: cyanocob(I)alamin


organism: Methanosarcina mazei


organism: Serratia plymuthica


organism: Salmonella enterica subsp. enterica serovar Typhimurium


organism: Salmonella enterica

assay_conditions_summary: pH 7; 310.15 K; cofactor ATP

proof_lines:
pH 7.0, 37°C, recombinant enzyme
at pH 7.0 and 37°C


enzyme_display_name: cysteine synthase

organism: Brassica juncea

substrate_name: O-acetyl-L-Ser

enzyme: cysteine synthase

substrate: O-acetyl-L-Ser


organism: Aeropyrum pernix

substrate_name: thiosulfate

proof_lines:
synthesis of S-sulfo-L-cysteine
sulfhydrylation of O-acetyl-L-serine

substrate: thiosulfate


organism: Arabidopsis thaliana

proof_lines: pH 7.5, 25°C, recombinant complex-bound enzyme

proof_lines: isoform B, pH 7.5, 25°C, Michaelis-Menten kinetics


organism: Enterobacter sp.

substrate_name: NaCN

proof_lines:
pH 7.4, 30°C
pH 7.4, 30°C, cosubstrate-dependent

substrate: NaCN


organism: Capsicum annuum


organism: Escherichia coli

proof_lines: pH 7.5, 25°C, enzyme bound to serine acetyltransferase


organism: Corynebacterium glutamicum


organism: Hordeum vulgare


organism: Fusobacterium nucleatum subsp. nucleatum

substrate_name: O-acetyl-L-serine

substrate: O-acetyl-L-serine


organism: Geobacillus stearothermophilus


organism: Cavia porcellus

substrate_name: testosterone

assay_conditions_summary: cofactor NADP+

substrate: testosterone


organism: [Clostridium] innocuum


organism: Comamonas testosteroni

substrate_name: 3-pyridinealdehyde adenine dinucleotide

assay_conditions_summary: cofactor NAD+

substrate: 3-pyridinealdehyde adenine dinucleotide


organism: Rattus norvegicus

proof_lines: 3beta-hydroxy-5alpha-androstan-17-one as substrate, 3beta-hydroxysteroid dehydrogenase activity

proof_lines: 5alpha-dihydrotestosterone as substrate, 3-beta-hydroxysteroid dehydrogenase activity

proof_lines: estrone as substrate, 17beta-hydroxysteroid dehydrogenase activity


organism: Paracoccus denitrificans


organism: Penicillium chrysogenum


organism: Raphanus sativus


organism: Saccharomyces cerevisiae

substrate_name: O-acetylhomoserine

substrate: O-acetylhomoserine


organism: Lathyrus sativus


organism: Leishmania major

substrate_name: Unknown

substrate: Unknown


organism: Leucaena leucocephala

substrate_name: hydrogen sulfide

substrate: hydrogen sulfide


organism: Methanosarcina thermophila


organism: Salmonella enterica subsp. enterica serovar Typhimurium

proof_lines:
25°C, 100 mM HEPES, pH 7.0
less than
complex-bound enzyme


organism: Solanum tuberosum

substrate_name: CN-

proof_lines:
pH 8.5, enzyme form PCS-2
pH 7.5, enzyme form PCS-1
pH 8.5, enzyme form PCS-1

substrate: CN-


organism: Thermus thermophilus


organism: Trichomonas vaginalis

proof_lines: 37°C, pH 7.8, cosubstrate: O-phosphoserine

proof_lines: 37°C, pH 7.8, cosubstrate: O-acetyl-L-Ser


organism: Trifolium repens


organism: Triticum aestivum


enzyme_display_name: polo kinase

organism: Homo sapiens

proof_lines: Ki, apparent dissociation constant, less than 0.0000005

enzyme: polo kinase


substrate_name: casein

substrate: casein


enzyme_display_name: kynurenine--oxoglutarate transaminase

organism: Penaeus monodon

substrate_name: L-kynurenine

enzyme: kynurenine--oxoglutarate transaminase

substrate: L-kynurenine


organism: Rattus norvegicus

proof_lines: pH 8.0, 37°C, recombinant enzyme, cosubstrate 2-oxoglutarate


organism: Hansenula schneggii

proof_lines:
pH 8.0, 37°C, cosubstrate L-kynurenine
pH 8.0, 37°C
pH 8.0. 25°C


proof_lines:
discontinuous assay, pH 7.5, 45°C
pH 7.5, 45°C
continuous assay, pH 7.5, 45°C


organism: Mus musculus

substrate_name: phenylpyruvate

substrate: phenylpyruvate


organism: Neurospora crassa

substrate_name: pyruvate

proof_lines:
pH 7.5, 37°C, cosubstrate L-kynurenine
pH 7.5, 37°C

substrate: pyruvate


organism: Bos taurus

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP


organism: Escherichia coli

substrate_name: deamido-NAD+

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NAD+

substrate: deamido-NAD+


organism: Katsuwonus pelamis

substrate_name: NAD+

substrate: NAD+


enzyme_display_name: N-acetylgalactosamine kinase

substrate_name: ATP

assay_conditions_summary: pH 7; 298.15 K; cofactor ATP

proof_lines:
recombinant enzyme
pH 7.0, 25°C

enzyme: N-acetylgalactosamine kinase

substrate: ATP


organism: Sus scrofa


organism: Pyrococcus horikoshii

substrate_name: nicotinamide ribonucleotide

assay_conditions_summary: pH 7; 343.15 K; cofactor ATP

substrate: nicotinamide ribonucleotide


organism: Saccharolobus solfataricus

substrate_name: NMN

assay_conditions_summary: pH 7.4; 343.15 K; cofactor ATP

substrate: NMN


organism: Saccharomyces cerevisiae


assay_conditions_summary: pH 10; 298.15 K; cofactor ATP

proof_lines:
25°C, pH 10
25°C, pH 5.15
immobilized enzyme


organism: Acanthamoeba castellanii

substrate_name: alpha-D-glucose 1-phosphate

substrate: alpha-D-glucose 1-phosphate


substrate_name: diphosphate

substrate: diphosphate


organism: Cucumis sativus

substrate_name: UTP

proof_lines:
pH 7.2, 30°C
diphosphate in form of Mg-diphosphate

substrate: UTP


substrate_name: TTP

substrate: TTP


organism: Ganoderma lucidum

substrate_name: UDP-glucose

proof_lines: pH 7.5, temperature not specified in the publication

substrate: UDP-glucose


proof_lines: non-galactosemic phenotype, pH 7.8, 37°C


organism: Hordeum vulgare


organism: Hordeum vulgare subsp. vulgare


organism: Lilium longiflorum


organism: Oryctolagus cuniculus


organism: Pseudomonas aeruginosa


assay_conditions_summary: pH 7.6; 310.15 K; cofactor ATP

proof_lines:
pH 7.6, 37°C
pH 7.6, 70°C
cosubstrate UTP, pH 7.6, 37°C


substrate_name: methylenebisphosphonate

substrate: methylenebisphosphonate


organism: Solanum tuberosum

proof_lines: at physiological substrate concentration, pH 8.5, 37°C

proof_lines: at physiological substrate concentrations, pH 8.5, 37°C


organism: Sorghum bicolor


organism: Streptococcus pneumoniae


organism: Triticum aestivum

assay_conditions_summary: pH 9

proof_lines: pH 9.0, temperature not specified in the publication


enzyme_display_name: aryl sulfotransferase

organism: Drosophila melanogaster

substrate_name: 3'-phosphoadenylyl sulfate

proof_lines: isoform ST4, in 50 mM sodium phosphate, pH 6.8, at 37°C

proof_lines: isoform ST1, in 50 mM sodium phosphate, pH 6.8, at 37°C

proof_lines: isoform ST3, in 50 mM sodium phosphate, pH 6.8, at 37°C

enzyme: aryl sulfotransferase

substrate: 3'-phosphoadenylyl sulfate


organism: Euglena gracilis


organism: Haliangium ochraceum


organism: Ictalurus punctatus

proof_lines:
intestinal enzyme, pH 7.0, 35°C
liver enzyme, pH 7.0, 35°C


organism: Mus musculus

substrate_name: p-nitrophenol

assay_conditions_summary: pH 7.4; 310.15 K; buffer 50 mM Tris/HCl

proof_lines: isoform Sult1a1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37°C

substrate: p-nitrophenol


organism: Arachis hypogaea

proof_lines:
enzyme from plasma membrane
enzyme from Golgi apparatus


organism: Cajanus cajan

proof_lines:
enzyme N-I
enzyme N-I, enzyme N-II
enzyme N-II


organism: Cavia porcellus


organism: Columba livia

assay_conditions_summary: pH 7; 310.15 K; cofactor ADP

proof_lines:
37°C, pH 7.0, presence of 1 mM ADP
37°C, pH 7.0


organism: Crocodylus siamensis

substrate_name: 5'-inosinic acid

substrate: 5'-inosinic acid


organism: Danio rerio

substrate_name: MgAMP

substrate: MgAMP


organism: Dosidicus gigas

substrate_name: AMP

assay_conditions_summary: 323.15 K; buffer note

proof_lines: in 40 mM sodium citrate buffer, 0.1 mM dithiothreitol, 110 mM NaCl, 20 mM MgCl2, and 20 mM CaCl2, at 50°C

substrate: AMP


organism: Gadus macrocephalus


organism: Salvator rufescens


organism: Sebastes inermis

proof_lines:
membrane-bound enzyme
cytosolic enzyme


organism: Streptomyces sp. NE5-10


organism: Streptomyces sp. Y2F8-2


organism: Gallus gallus


organism: Homo sapiens

assay_conditions_summary: pH 8.5; 298.15 K; cofactor ATP

proof_lines:
presence of 3 mM ATP
at pH 8.5
pH 8.3, 25°C


organism: Metamycoplasma arthritidis


proof_lines: pH 7.4, 37°C, enzyme from dystrophic muscle


organism: Mycoplasmopsis fermentans

proof_lines: pH 8.5, 37°C, strain KL8, strain BRO and strain M106


organism: Mycoplasmopsis pulmonis


organism: Neurospora crassa


organism: Oncorhynchus masou


organism: Oryctolagus cuniculus


organism: Rattus norvegicus

proof_lines:
synaptic membrane-bound
brain cytosol
enzyme from glioblastoma cells


organism: Saccharomyces cerevisiae

proof_lines: 5-nucleotidase Sdt1, pH not specified in the publication, temperature not specified in the publication


organism: Tritrichomonas suis


organism: Varanus gouldii


organism: Zea mays


organism: Sus scrofa


organism: Torpedo marmorata


organism: Trichomonas gallinae

assay_conditions_summary: pH 7.2; 310.15 K; buffer 50 mM Tris

proof_lines: in 50 mM Tris buffer (pH 7.2) and 3.0 mM MgCl2, at 37°C


organism: Trichomonas vaginalis


organism: Bifidobacterium bifidum


organism: Elizabethkingia meningoseptica

substrate_name: 4-nitrophenyl alpha-L-fucoside

substrate: 4-nitrophenyl alpha-L-fucoside


organism: Prunus dulcis

substrate_name: lacto-N-fucopentaitol III

substrate: lacto-N-fucopentaitol III


enzyme_display_name: chitinase

organism: Aeromonas media

substrate_name: chitopentaose

enzyme: chitinase

substrate: chitopentaose


organism: Annona cherimola

substrate_name: 4-methylumbelliferyl chitobioside

substrate: 4-methylumbelliferyl chitobioside


organism: Autographa californica


organism: Bacillus cereus

proof_lines: 37°C, C-terminal truncated enzyme ChiCWDELTAFC


organism: Bacillus licheniformis

substrate_name: colloidal chitin

substrate: colloidal chitin


organism: Bacillus sp. (in: firmicutes)

substrate_name: chitin

substrate: chitin


organism: Caldicellulosiruptor acetigenus


organism: Coccidioides immitis

substrate_name: chitohexaose

substrate: chitohexaose


organism: Coprinopsis cinerea


organism: Ficus pumila var. awkeotsang


organism: Homo sapiens


organism: Hydrogenophilus hirschii


organism: Leishmania major


organism: Metarhizium anisopliae


organism: Nepenthes alata

substrate_name: ethlene glycol chitin

substrate: ethlene glycol chitin


organism: Niallia circulans


organism: Oryza sativa


organism: Paenibacillus sp.


organism: Paenibacillus thermoaerophilus


organism: Paenibacillus timonensis


organism: Penicillium ochrochloron


organism: Penicillium oxalicum


organism: Pennahia argentata

substrate_name: glycol chitin

substrate: glycol chitin


organism: Saccharomyces cerevisiae

proof_lines: expressed in N-acetylglucosamine equivalents


organism: Scorpaena scrofa


organism: Serratia marcescens


organism: Setaria cervi


organism: Stenotrophomonas maltophilia


organism: Stomoxys calcitrans


organism: Streptomyces chilikensis

proof_lines: 37°C, pH not specified in the publication


organism: Streptomyces sp.

proof_lines: Chi-A Chi-B


organism: Tenebrio molitor


organism: Anabaena sp.

substrate_name: diphosphate

source_db: oed

substrate: diphosphate

source: oed


organism: Arabidopsis thaliana


substrate_name: H2O; Diphosphate

substrate: H2O; Diphosphate


proof_lines: pH 7.5, 22°C, recombinant truncated mutant AtPPA1-DELTA(1-29)


proof_lines: pH 7.5, 22°C, recombinant wild-type enzyme


proof_lines: pH 7.5, 25°C, isozyme PPa4 in a complex with Mg2+


organism: Bacillus subtilis

substrate_name: imidodiphosphoric acid

proof_lines: pH 7.2, 25°C, wild-type enzyme, kinetics

substrate: imidodiphosphoric acid


organism: Bacteroides thetaiotaomicron


proof_lines: pH 7.5, 25°C, recombinant wild-type BT2127


organism: Chlamydomonas reinhardtii

substrate_name: Diphosphate; H2O

substrate: Diphosphate; H2O


organism: Clostridium perfringens


organism: Cyanidium caldarium


organism: Cyanophora paradoxa


organism: Desulfitobacterium hafniense


assay_conditions_summary: pH 7.2; 310.15 K; cofactor ATP

proof_lines: pH 7.2, 37°C, mutant R276E, in presence of 0.5 mM AMP

proof_lines: pH 7.2, 37°C, mutant R276E, in presence of 0.5 mM ATP

proof_lines: pH 7.2, 37°C, mutant R276E, in presence of 0.5 mM ADP


proof_lines: pH 7.2, 37°C, mutant R276K, in presence of 0.5 mM ATP

proof_lines: pH 7.2, 37°C, mutant R276K, in presence of 0.5 mM Ap4A


proof_lines: pH 7.2, 37°C, mutant R276A, in presence of 0.5 mM ATP

proof_lines: pH 7.2, 37°C, mutant R276A, in presence of 0.5 mM AMP

proof_lines: pH 7.2, 37°C, mutant R276A, in presence of 0.5 mM Ap4A


proof_lines: pH 7.2, 37°C, wild-type enzyme, in presence of 0.5 mM AMP

proof_lines: pH 7.2, 37°C, wild-type enzyme, in presence of 0.5 mM ADP

proof_lines: pH 7.2, 37°C, wild-type enzyme, in presence of 0.5 mM Ap4A


organism: Escherichia coli

substrate_name: Unknown

proof_lines:
pH 7.4, 25°C, with 0.05 mM Mn2+
pH 7.4, 25°C, with 5 mM Mn2+
recombinant enzyme, pH 7.5, 37°C

substrate: Unknown


organism: Ethanoligenens harbinense


organism: Euglena gracilis


organism: Haloferax volcanii

proof_lines:
recombinant enzyme, pH 8.5, 42°C
recombinant enzyme, pH 8.5, 25°C


organism: Helicobacter pylori

proof_lines:
recombinant enzyme
Raw SKiD Env: Temp=37°C, pH=8
Raw SKiD Env: Temp=-----, pH=-----


proof_lines: pH 8.0, 37°C, recombinant wild-type enzyme


organism: Homo sapiens

substrate_name: cAMP; H2O

substrate: cAMP; H2O


organism: Legionella pneumophila subsp. pneumophila

substrate_name: 4-nitrophenyl phosphate

proof_lines: pH 7.2, temperature not specified in the publication

substrate: 4-nitrophenyl phosphate


organism: Leishmania major

proof_lines:
pH 7.5, in the presence of Ca2+
pH 7.5, in the presence of Mg2+


organism: Medicago truncatula


proof_lines: pH 7.5, 22°C, recombinant wild-type enzyme


organism: Methanothrix thermoacetophila


proof_lines: pH 8.0, 37°C, with Mn2+ as metal cofactor


organism: Mycobacterium tuberculosis


proof_lines: pH 7.5, 20°C, mutant enzyme Ec-Mt-PPase with the missing part of the polypeptide chain (N-terminal region) compensated with a fragment of PPase from Escherichia coli


organism: Ochromonas danica


organism: Plasmodium falciparum


substrate_name: ATP

substrate: ATP


proof_lines: pH 7.2, 37°C, recombinant wild-type enzyme


organism: Pseudanabaena sp.


organism: Pyrococcus furiosus

substrate_name: UDP-glucose

substrate: UDP-glucose


substrate_name: ADP

assay_conditions_summary: pH 9.5; 368.15 K; cofactor ADP

substrate: ADP


substrate_name: UDP

substrate: UDP


substrate_name: IDP

substrate: IDP


organism: Rhodopseudomonas viridis


organism: Rhodospirillum rubrum


organism: Saccharolobus islandicus


organism: Streptococcus gordonii


proof_lines: pH 9.0, 25°C, 5 mM Mg2+, wild-type enzyme

proof_lines: recombinant wild-type enzyme, pH 9.0, 25°C


substrate_name: ITP

substrate: ITP


substrate_name: Triphosphate

substrate: Triphosphate


assay_conditions_summary: pH 9.5; 368.15 K; cofactor ATP


substrate_name: CTP

substrate: CTP


substrate_name: GDP

substrate: GDP


substrate_name: CDP

substrate: CDP


substrate_name: GTP

substrate: GTP


substrate_name: UTP

substrate: UTP


substrate_name: diphosphate

substrate: diphosphate


organism: Pyrococcus horikoshii

proof_lines:
pH 7.5, 60°C, recombinat enzyme
pH 8.0, 90°C


organism: Rhipicephalus microplus


organism: Synechocystis sp.

proof_lines:
recombinant enzyme
native enzyme


organism: Thermococcus onnurineus


proof_lines: wild type enzyme, in 50 mM MOPS (pH 6.5), in the presence of Co2+, at 80°C

proof_lines: wild type enzyme, in 50 mM MOPS (pH 6.5), in the presence of Ni2+, at 80°C

proof_lines: wild type enzyme, in 50 mM MOPS (pH 6.5), in the presence of Mg2+, at 80°C


proof_lines: pH and temperature not specified in the publication


organism: Thermoplasma acidophilum


proof_lines: recombinant mutants I260V and I260E, pH 9.0, 25°C


organism: Sulfolobus acidocaldarius

assay_conditions_summary: pH 3; 328.15 K; cofactor ATP

proof_lines:
pH 3.0, 55°C
Raw SKiD Env: Temp=55°C, pH=3


organism: Arabidopsis thaliana

substrate_name: PPi

source_db: uniprot

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

data_origin: experimental

substrate: PPi

source: uniprot


substrate_name: pyrophosphate PPi (at 37 degrees Celsius)


substrate_name: pyrophosphate

substrate: pyrophosphate


organism: Bacillus subtilis (strain 168)


organism: Blastochloris viridis

sequence: MRIDAIDXA


organism: Chlamydomonas reinhardtii


organism: Clostridium perfringens (strain 13 / Type A)


organism: Cyanophora paradoxa

sequence: XAITAEPVGTPETLEYRVFIQKDGK


organism: Euglena gracilis

sequence: MDTKLGLVEEEAAN


organism: Methanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT)

substrate_name: diphosphate (with manganese as cofactor)


organism: Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


organism: Ochromonas danica

sequence: XYAFGINGTXRI


organism: Pseudanabaena sp. (strain PCC 6903)


organism: Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)


organism: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

substrate_name: CTP (at pH 8 and at 70 degrees Celsius)

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

assay_conditions_summary: pH 8; cofactor ATP

substrate: CTP (at pH 8 and at 70 degrees Celsius)


organism: Vigna radiata


substrate_name: Unknown

substrate: Unknown


organism: Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)

substrate_name: diphosphate (at 42 degrees Celsius)

proof_lines: Displays non-Michaelis-Menten kinetics for PPi hydrolysis. Hill coefficients indicate cooperative binding to PPi and Mg(2+).

substrate: diphosphate (at 42 degrees Celsius)


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: diphosphate (at pH 7.2)

substrate: diphosphate (at pH 7.2)


organism: Escherichia coli

proof_lines:
pH 7.5, 25°C, mutant K112Q
pH 7.5, 25°C, trimeric mutant K112Q


proof_lines: pH 6.5, wild type, resynthesis of diphosphate

proof_lines: pH 8, wild type, hydrolysis of diphosphate


assay_conditions_summary: pH 8

proof_lines: pH 7.2, D97E, resynthesis of diphosphate


substrate_name: Mg-diphosphate

proof_lines:
pH 7.2, H140Q enzyme, trimeric
pH 7.2, H140Q enzyme, hexameric
pH 8, H140Q enzyme, trimeric

substrate: Mg-diphosphate


proof_lines:
pH 8, hexameric, H136Q enzyme
pH 7.2, hexameric, H136Q enzyme
pH 7.2, H136Q enzyme, trimeric


organism: Thermococcus chitonophagus

substrate_name: p-nitrophenol-beta-D-galactopyranoside

substrate: p-nitrophenol-beta-D-galactopyranoside


organism: Trichoderma atroviride

substrate_name: colloidal chitin

substrate: colloidal chitin


organism: Trichoderma harzianum


organism: Triticum aestivum

substrate_name: chitin

proof_lines: chitin concentration calculated as N-acetylglucosamine

substrate: chitin


enzyme_display_name: limit dextrinase

organism: Sorghum bicolor

substrate_name: pullulan

enzyme: limit dextrinase

substrate: pullulan


organism: Vicia faba


enzyme_display_name: pyroglutamyl-peptidase II

organism: Bos taurus

enzyme: pyroglutamyl-peptidase II


organism: Cavia porcellus

substrate_name: Thyroliberin

substrate: Thyroliberin


organism: Rattus norvegicus


organism: Avena sativa


organism: Hordeum vulgare


organism: Lathyrus oleraceus

substrate_name: amylopectin beta-limit dextrin

substrate: amylopectin beta-limit dextrin


organism: Oryza sativa


organism: Vibrio alginolyticus


enzyme_display_name: coagulation factor XIIa

organism: Homo sapiens

proof_lines:
0.05 M Tris-HCl, pH 7.8, 0.117 M NaCl
0.05 M Tris-HCl, pH 8.0

enzyme: coagulation factor XIIa


organism: Sus scrofa

substrate_name: 5-oxoprolyl 2-naphthylamide

proof_lines: pH 7.5, 37°C, hydrolysis of pyroglutamyl-His-Pro-7-amido-4-methylcoumarin

substrate: 5-oxoprolyl 2-naphthylamide


enzyme_display_name: hepsin

proof_lines:
peptide sequence from PAR-2
peptide sequence from matriptase
peptide sequence from filaggrin

enzyme: hepsin


organism: Mus musculus


enzyme_display_name: subtilisin

organism: Bacillus amyloliquefaciens

substrate_name: MeO-succinyl-Ala-Ala-Phe 4-nitroanilide

proof_lines: succinyl-Ala-Ala-Pro-Phe 4-nitroanilide, subtilisin BPN

enzyme: subtilisin

substrate: MeO-succinyl-Ala-Ala-Phe 4-nitroanilide


organism: Bacillus intermedius


organism: Bacillus licheniformis

substrate_name: Z-Ala-Ala-Leu-p-nitroanilide

proof_lines:
in 20% DMF
in 2% DMF
in 20% DMSO

substrate: Z-Ala-Ala-Leu-p-nitroanilide


organism: Thermococcus kodakarensis

assay_conditions_summary: pH 7.5; 293.15 K; buffer 50 mM Tris-HCl

proof_lines: subtilisin-like serine protease, at 20°C, in 50 mM Tris-HCl buffer, pH 7.5

proof_lines: subtilisin-like serine protease, at 80°C, in 50 mM Tris-HCl buffer, pH 7.5

proof_lines: subtilisin, at 20°C, in 50 mM Tris-HCl buffer, pH 8.0


organism: Bacillus sp. (in: firmicutes)

substrate_name: Succinyl-Ala-Ala-Ala 4-nitroanilide

proof_lines:
subtilisin Carlsberg
subtilisin Sendai
subtilisin DY

substrate: Succinyl-Ala-Ala-Ala 4-nitroanilide


organism: Bacillus subtilis

substrate_name: acetyl-L-Tyr

proof_lines:
30°C, pH 8.0
in 20% DMSO
in 2% DMSO

substrate: acetyl-L-Tyr


organism: Dichelobacter nodosus


organism: Fusarium equiseti


enzyme_display_name: HIV-2 retropepsin

organism: Human immunodeficiency virus 2

substrate_name: IPFAAAQQRK

enzyme: HIV-2 retropepsin

substrate: IPFAAAQQRK


enzyme_display_name: scytalidoglutamic peptidase

organism: Scytalidium lignicola

proof_lines: pH and temperature not specified in the publication

enzyme: scytalidoglutamic peptidase


organism: Melghiribacillus thermohalophilus

substrate_name: azocasein

proof_lines:
pH 10.0, 50°C
pH 10.0, 50°C, calculated by BRENDA

substrate: azocasein


organism: Shouchella clausii


organism: Solanum lycopersicum

substrate_name: Unknown

substrate: Unknown


organism: synthetic construct

assay_conditions_summary: pH 7.8; 298.15 K; buffer 10 mM potassium phosphate

proof_lines: 3.5 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C

proof_lines: subtilisin without bound lactose, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C

proof_lines: 2.2 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C


enzyme_display_name: penicillin amidase

substrate_name: Penicillin V

enzyme: penicillin amidase

substrate: Penicillin V


organism: Escherichia coli

substrate_name: (S)-N-acetylphenylglycine

substrate: (S)-N-acetylphenylglycine


organism: Fusarium oxysporum


organism: Fusarium sp.


organism: Achromobacter sp.

substrate_name: penicillin G

substrate: penicillin G


organism: Achromobacter xylosoxidans


organism: Actinoplanes utahensis


organism: Alcaligenes faecalis

substrate_name: 6-nitro-3-phenylacetamidobenzoic acid

proof_lines:
pH 7.5, 25°C, enzyme form PA5..3
pH 7.5, 25°C, enzyme form PA5..5
pH 7.0, 25°C

substrate: 6-nitro-3-phenylacetamidobenzoic acid


organism: Kluyvera cryocrescens

substrate_name: benzylpenicillin

proof_lines: pH 7.5, 40°C, presence of 10 mM phenylglyoxal

proof_lines: pH 7.5, 40°C, presence of 50 mM 2,3-butanedione

proof_lines: unmodified enzyme, enzyme modified with N-bromosuccinamide or enzyme modified with 2-hydroxy 5-nitrobenzylbromide

substrate: benzylpenicillin


organism: Lysinibacillus sphaericus


organism: Mucor griseocyanus


organism: Priestia megaterium


organism: Providencia rettgeri


organism: Pseudobacillus badius

substrate_name: ampicillin

substrate: ampicillin


organism: Pseudomonas melanogenum

substrate_name: cephadrine

substrate: cephadrine


organism: Streptomyces sp.


enzyme_display_name: N-acyl-D-amino-acid deacylase

organism: Streptomyces olivaceus

substrate_name: N-Acetyl-D-Trp

enzyme: N-acyl-D-amino-acid deacylase

substrate: N-Acetyl-D-Trp


organism: Trichoderma sp.

substrate_name: N-acetyl-D-methionine

substrate: N-acetyl-D-methionine


organism: Achromobacter denitrificans

substrate_name: N-Chloroacetyl-D-Val

substrate: N-Chloroacetyl-D-Val


substrate_name: N-Acetyl-D-Leu

substrate: N-Acetyl-D-Leu


substrate_name: N-Acetyl-D-Met

proof_lines:
soluble enzyme
immobilized enzyme

substrate: N-Acetyl-D-Met


organism: Rhodococcus armeniensis

substrate_name: N-acetyl-D-leucine

substrate: N-acetyl-D-leucine


enzyme_display_name: arylacetonitrilase

enzyme: arylacetonitrilase


organism: Alcaligenes sp. MTCC 10675

substrate_name: mandelonitrile

substrate: mandelonitrile


organism: Arabidopsis thaliana


organism: Aspergillus niger

substrate_name: phenyl acetonitrile

substrate: phenyl acetonitrile


organism: Rhizorhabdus wittichii

substrate_name: phenylglycinonitrile

substrate: phenylglycinonitrile


organism: Trichophyton benhamiae


organism: Bradyrhizobium japonicum

substrate_name: phenylacetonitrile

substrate: phenylacetonitrile


organism: Brassica napus

substrate_name: phenylpropionitrile

substrate: phenylpropionitrile


organism: Burkholderia cenocepacia


organism: Fusarium haematococcum


organism: Luminiphilus syltensis


organism: Neurospora crassa


organism: Pseudomonas fluorescens


organism: Pseudomonas putida


enzyme_display_name: inorganic diphosphatase

organism: Aspergillus oryzae

substrate_name: diphosphate

proof_lines:
pH 6.9
pH 3

enzyme: inorganic diphosphatase

substrate: diphosphate


organism: Blastochloris viridis


organism: Bos taurus

substrate_name: Imidodiphosphate

substrate: Imidodiphosphate


organism: Capsicum annuum


organism: Cricetinae

proof_lines: diphosphate at 1 mM, enzyme from astroblasts

proof_lines: diphosphate at 1 mM, enzyme from neuroblasts


organism: Desulfovibrio desulfuricans

assay_conditions_summary: pH 6


organism: Dunaliella salina


organism: Entamoeba histolytica


organism: Escherichia coli

proof_lines: pH 7.5, 25°C, hexameric mutant E145Q, 5 mM Mg2+


organism: Gossypium hirsutum

substrate_name: Unknown

proof_lines: pH 7.2, 37°C, fluoride inhibition kinetics

substrate: Unknown


organism: Helicobacter pylori

substrate_name: Mg-diphosphate

substrate: Mg-diphosphate


organism: Homo sapiens


organism: Hypophthalmichthys nobilis


organism: Methanothermobacter thermautotrophicus


organism: Streptococcus salivarius


organism: Sulfolobus acidocaldarius


organism: Sus scrofa


organism: Thermococcus onnurineus


organism: Mus sp.


organism: Neomoorella thermoacetica


organism: Saccharomyces cerevisiae

substrate_name: ATP

assay_conditions_summary: pH 7.2; 298.15 K; cofactor ATP

proof_lines: less than 0.005/sec, pH 7.2, 25°C, in the presence of Mg2+

substrate: ATP


organism: Saccharum officinarum

proof_lines:
isoform 1
isoform 2


enzyme_display_name: orotidine-5'-phosphate decarboxylase

organism: Crithidia luciliae

substrate_name: orotidine 5'-phosphate

proof_lines:
membrane-bound enzyme
solubilized enzyme

enzyme: orotidine-5'-phosphate decarboxylase

substrate: orotidine 5'-phosphate


assay_conditions_summary: pH 7

proof_lines: pH 7, constant above pH 7, declines below pH 7


proof_lines: in 50 mM Tris (pH 7.5), 20 mM dithiothreitol, and 40 mM NaCl, at 22°C


proof_lines: orotidine-5-phosphate decarboxylase domain of the bifunctional enzyme UMP synthase


substrate_name: 4-Thioorotidine 5'-phosphate

proof_lines: 22-25°C, pH 7.2, recombinant ODCase expressed in Escherichia coli

proof_lines: pH 7.2, 25°C, not affected by NaCl concentration

substrate: 4-Thioorotidine 5'-phosphate


substrate_name: Orotidine

proof_lines: value is higher than 30 mM, pH 7.2, 23°C, mutant enzyme C155S

substrate: Orotidine


substrate_name: 5-Fluoroorotidine 5'-phosphate

proof_lines: mutant R235A, pH 7.1, 25°C, presence of 0.1 M NaCl

proof_lines: mutant R235A, pH 7.1, 25°C, presence of 0.1 M NCl

substrate: 5-Fluoroorotidine 5'-phosphate


organism: Staphylococcus aureus


proof_lines: in 50 mM Tris, pH 7.5, 20 mM dithiothreitol, and 40 mM NaCl, at 55°C


organism: Mus musculus

proof_lines: orotidine-5-phosphate decarboxylase domain of the bifunctional enzyme UMP synthase, 20°C

proof_lines: orotidine-5-phosphate decarboxylase domain of the bifunctional enzyme UMP synthase, 37°C


organism: Plasmodium falciparum


organism: Pseudomonas aeruginosa


enzyme_display_name: phosphoenolpyruvate carboxylase

organism: Cicer arietinum

enzyme: phosphoenolpyruvate carboxylase


organism: Coccochloris peniocystis


organism: Coleataenia prionitis


organism: Annona cherimola

substrate_name: phosphoenolpyruvate

proof_lines: pH 7.8, enzyme from mesocarp, stored in air

proof_lines: pH 7.8, enzyme from mesocarp, stored in 20% CO2

substrate: phosphoenolpyruvate


organism: Bienertia sinuspersici

proof_lines: leaves are collected after 5 h in the dark, pH: 8.0

proof_lines: leaves are collected after 5 h in the light, pH: 8.0