enzyme_display_name: atrolysin C

organism: Crotalus atrox

substrate_name: acetyl-Val-Ala-Leu-Gly-Gly

proof_lines: isozyme c, acetyl-Val-Ala-Leu-Gly-Ala, 2-aminobenzoyl-Ala-Gly-Leu-Ala 4-nitrobenzyl amide

enzyme: atrolysin C

substrate: acetyl-Val-Ala-Leu-Gly-Gly


enzyme_display_name: D-sedoheptulose-7-phosphate isomerase

organism: Mycobacterium tuberculosis

substrate_name: D-sedoheptulose 7-phosphate

source_db: brenda

ki_display: Not reported

kcat_over_km_display: Not reported

protein_accession_database: UniProt

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution

enzyme: D-sedoheptulose-7-phosphate isomerase

substrate: D-sedoheptulose 7-phosphate

source: brenda


enzyme_display_name: complement factor D

organism: Homo sapiens

substrate_name: Benzyloxycarbonyl-Lys-thiobenzyl ester

enzyme: complement factor D

substrate: Benzyloxycarbonyl-Lys-thiobenzyl ester


mutation_signature: Unknown

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


enzyme_display_name: monacolin J acid methylbutanoate transferase

organism: Aspergillus terreus

substrate_name: monacolin J

source_db: uniprot

kcat_display: Not reported

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: kcat is 0.62 min(-1) for simvastatin synthesis.

substrate: monacolin J

source: uniprot


organism: Aspergillus terreus (strain NIH 2624 / FGSC A1156)

enzyme: monacolin J acid methylbutanoate transferase


organism: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

substrate_name: PRPP

proof_lines: kcat is 0.23 sec(-1) with pABA as substrate.

substrate: PRPP


organism: Escherichia coli (strain K12)

substrate_name: phospho-Hep2-Kdo2-lipid A

proof_lines: kcat is 0.41 sec(-1) with phospho-Hep2-Kdo2-lipid A as substrate. kcat is 0.028 sec(-1) with O-deacylated phospho-Hep2-Kdo2-lipid A. kcat is 0.07 sec(-1) with fully deacylated phospho-Hep2-Kdo2-lipid A.

substrate: phospho-Hep2-Kdo2-lipid A


substrate_name: UDP-N-acetylglucosamine

proof_lines: kcat is 0.21 min(-1) with UDP-N-acetylglucosamine. kcat is 0.20 min(-1) with dolichol phosphate.

substrate: UDP-N-acetylglucosamine


enzyme_display_name: phosphoribosyl-AMP cyclohydrolase

organism: Methanococcus vannielii

substrate_name: phosphoribosyl-AMP

enzyme: phosphoribosyl-AMP cyclohydrolase

substrate: phosphoribosyl-AMP


organism: Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)


enzyme_display_name: prenyl-diphosphate phosphatase

assay_conditions_summary: pH 8

proof_lines: kcat is 0.78 sec(-1) for (2E)-geranyl diphosphate as substrate (at pH 8.0 and 22 degrees Celsius) (PubMed:38944687). kcat is 0.55 sec(-1) with (R)-5-diphosphomevalonate as substrate (at pH 8.0 and 22 degrees Celsius) (PubMed:38944687). kcat

enzyme: prenyl-diphosphate phosphatase


substrate_name: dGTP (at pH 7.5)

proof_lines: Has a 10-fold higher catalytic efficiency toward dGTP compared to 8-oxo-dGTP. kcat is 0.58 sec(-1) with dGTP as substrate (at pH 8.0 and 22 degrees Celsius) (PubMed:38944687). kcat is 0.72 sec(-1) with (2E)-geranyl diphosphate as substrate

substrate: dGTP (at pH 7.5)


organism: Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)

substrate_name: dimethylallyl diphosphate (DMAPP) (at pH 8.5 and 30 degrees Celsius)

proof_lines: kcat is 0.139 sec(-1) with DMAPP as substrate. kcat is 0.681 sec(-1) with as IPP substrate. kcat is 0.395 sec(-1) with GPP as substrate. kcat is 0.298 sec(-1) with FPP as substrate.


enzyme_display_name: imidazoleglycerol-phosphate dehydratase

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: imidazoleglycerol-phosphate (at pH 7.7 and 37 degrees Celsius)

proof_lines: kcat is 1000 sec(-1) with imidazoleglycerol-phosphate as substrate (at pH 7.7 and 37 degrees Celsius).

enzyme: imidazoleglycerol-phosphate dehydratase


enzyme_display_name: L-ascorbate peroxidase

organism: Galdieria partita

substrate_name: L-ascorbate

enzyme: L-ascorbate peroxidase

substrate: L-ascorbate


organism: Methanothermobacter thermautotrophicus


proof_lines:
wild type enzyme, at pH 6.8 and 70°C
wild type enzyme, at pH 6.8 and 50°C


enzyme_display_name: mycoredoxin

organism: Corynebacterium glutamicum

substrate_name: 2-hydroxyethyl disulfide-mycothiol

proof_lines: Ncgl2478mutant enzyme C21S, at pH 7.6 and 30°C

enzyme: mycoredoxin

substrate: 2-hydroxyethyl disulfide-mycothiol


proof_lines: wild type enzyme Ncgl2478, at pH 7.6 and 30°C

proof_lines: wild type enzyme NCgl0018, at pH 7.5 and 37°C

proof_lines: wild type enzyme Mrx1, at pH 7.6 and 30°C


proof_lines: Ncgl2478mutant enzyme C24S, at pH 7.6 and 30°C


organism: Lathyrus oleraceus

substrate_name: guaiacol

proof_lines:
will-type enzyme
enzyme form C
enzyme form B

substrate: guaiacol


organism: Leishmania major

substrate_name: ascorbate

substrate: ascorbate


substrate_name: cytochrome c

substrate: cytochrome c


proof_lines: wild-type, Cys32Ser mutant and DTNB-modified enzyme


proof_lines: NCgl0018 mutant enzyme C107S, at pH 7.5 and 37°C


proof_lines: NCgl0018 mutant enzyme C104S, at pH 7.5 and 37°C


enzyme_display_name: 3-methylquercetin 7-O-methyltransferase

organism: Populus sp.

substrate_name: kaempferol

assay_conditions_summary: pH 7.5; 310.15 K; buffer 10 mM Tris/HCl

proof_lines: wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C

enzyme: 3-methylquercetin 7-O-methyltransferase

substrate: kaempferol


proof_lines: mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C


substrate_name: D-mannose 6-phosphate

substrate: D-mannose 6-phosphate


enzyme_display_name: [Src-family] C-terminal protein kinase

substrate_name: [Src]-(L-tyrosine)

proof_lines: catalytic domain of the enzyme with all grafted Src elements (DELTAI255/DELTAL262/M129R/C-loop/alpha-helix C region/beta4.5 turn), at pH 8.0 and 30°C

enzyme: [Src-family] C-terminal protein kinase

substrate: [Src]-(L-tyrosine)


proof_lines: catalytic domain of the enzyme, at pH 8.0 and 30°C


organism: Brassica rapa


organism: Chlorella vulgaris


organism: Cucumis metuliferus


enzyme_display_name: cathepsin F

organism: Homo sapiens

proof_lines: pH 6.5, room temperature, wild-type enzyme

enzyme: cathepsin F


proof_lines: pH 6.5, room temperature, mutant enzyme N97Q/N108Q/N170Q


organism: Euglena gracilis

assay_conditions_summary: pH 7; 295.15 K; buffer 50 mM potassium phosphate

proof_lines: recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C


organism: Glycine max

substrate_name: pyrogallol

proof_lines:
recombinant enzyme
recombinant enzyme 1
recombinant enzyme 2

substrate: pyrogallol


organism: Helicoverpa zea


organism: Musa x paradisiaca


organism: Nicotiana tabacum

proof_lines:
pH 6.4, untreated cells
pH 6.4, heat-shock cells


organism: Oryza sativa

proof_lines:
pH 7.0, APX 2
pH 6.5, APX 1


organism: Pallavicinia lyellii

proof_lines: in 50 mM potassium phosphate (pH 7.0), at 22°C


organism: Solanum tuberosum


organism: Zea mays


organism: Panicum virgatum

assay_conditions_summary: pH 7

proof_lines: pH 7.0, temperature not specified in the publication. Hill coefficient n is 1.0

proof_lines: pH 7.0, temperature not specified in the publication. Non-Michaelis-Menten kinetics, Hill ccoefficient n is 1.6


organism: Raphanus sativus


organism: Ricinus communis


organism: Saccharomyces cerevisiae

km_display: Not reported


enzyme_display_name: carotenoid isomerooxygenase

organism: Plesiocystis pacifica SIR-1

enzyme: carotenoid isomerooxygenase


enzyme_display_name: 2,4-dichlorophenol 6-monooxygenase

organism: Soil bacterium

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

enzyme: 2,4-dichlorophenol 6-monooxygenase


organism: Acinetobacter sp.

assay_conditions_summary: cofactor NADPH


organism: Burkholderia cepacia

substrate_name: NADPH

substrate: NADPH


organism: Cupriavidus necator

substrate_name: NADH

proof_lines:
+ 2,4-dichlorophenol
cosubstrate NADPH
cosubstrate NADH

substrate: NADH


organism: Pseudomonadota


organism: Bacillus amyloliquefaciens


organism: Chroomonas mesostigmatica


organism: Gynuella sunshinyii

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


organism: Nisaea denitrificans


organism: Ostreococcus virus


enzyme_display_name: N-terminal methionine N(alpha)-acetyltransferase NatB

organism: Homo sapiens

substrate_name: MLALISRRWGRPVGRRRRRPVRVYP

substrate: MLALISRRWGRPVGRRRRRPVRVYP


organism: Chrysosplenium americanum


organism: Prymnesium parvum

proof_lines: 22°C, pH not specified in the publication

proof_lines: 22°, pH not specified in the publication


organism: Ulva intestinalis


enzyme_display_name: very-long-chain ceramide synthase

substrate_name: behenoyl-CoA

assay_conditions_summary: pH 7.4; 310.15 K; cofactor CoA

enzyme: very-long-chain ceramide synthase

substrate: behenoyl-CoA


organism: Methanosarcina thermophila

proof_lines:
pH 4.8, 50°C
pH 4.8, 50°C, Ki(slope)


enzyme_display_name: glycogenin glucosyltransferase

organism: Oryctolagus cuniculus

substrate_name: UDP-glucose

proof_lines: non-glucosylated protein, mass sprectrometry assay

proof_lines: glucosylated protein, 14C-glucose incorporation assay

proof_lines: glucosylated protein, 4-10 interval, mass sprectrometry assay

enzyme: glycogenin glucosyltransferase

substrate: UDP-glucose


organism: Rattus norvegicus

substrate_name: n-dodecyl-beta-D-maltoside

substrate: n-dodecyl-beta-D-maltoside


organism: Gallus gallus

substrate_name: lactosylceramide

proof_lines:
at 0.2 mM
at 0.1 mM

substrate: lactosylceramide


substrate_name: CMP-N-acetylneuraminate

substrate: CMP-N-acetylneuraminate


organism: Mesocricetus auratus


organism: Oncorhynchus mykiss


enzyme_display_name: zeatin 9-aminocarboxyethyltransferase

organism: Lupinus luteus

substrate_name: O-glycosylzeatin

enzyme: zeatin 9-aminocarboxyethyltransferase

substrate: O-glycosylzeatin


enzyme_display_name: homospermidine synthase

organism: Acinetobacter tartarogenes

substrate_name: putrescine

enzyme: homospermidine synthase

substrate: putrescine


organism: Blastochloris viridis

proof_lines:
Vmax: 0.00132 mmol/min/mg
in presence of 1 mM spermidine
in presence of 1 mM putrescine


organism: Lathyrus sativus


enzyme_display_name: protein geranylgeranyltransferase type II

substrate_name: Unknown

ki_unit: mM

proof_lines: 9 microM (3,7,11-trimethyl-12-(7-nitrobenzo[1,2,5]oxadiazo-4-ylamino)-dodeca-2,6,19-trien-1) diphosphate, 3 microM Rab7, 3 microM Rab escort protein, 400 nM enzyme

proof_lines: mixed-type inhibition with geranylgeranyl diphosphate 1 as variable substrate, 50 mM sodium HEPES, pH 7.2, 5 mM MgCl2, 1 mM Nonidet P-40, 1 mM dithioerythritol, 4 microM Rab1a, 50 nM enzyme, 20 min, 37°C

substrate: Unknown


substrate_name: Rab protein

enzyme: protein geranylgeranyltransferase type II

substrate: Rab protein


organism: Saccharomyces cerevisiae


enzyme_display_name: asparagine--oxo-acid transaminase

organism: Mus musculus

substrate_name: L-methionine

proof_lines:
cosubstrate phenylpyruvate
cosubstrate 2-oxosuccinamate

enzyme: asparagine--oxo-acid transaminase

substrate: L-methionine


enzyme_display_name: [RNA-polymerase]-subunit kinase

substrate_name: ATP

assay_conditions_summary: pH 7.5; 300.15 K; cofactor ATP

proof_lines:
27°C, pH 7.5
30°C, pH 7.9, kinase CTDK1
30°C, pH 7.9, kinase CTDK2

enzyme: [RNA-polymerase]-subunit kinase

substrate: ATP


substrate_name: GTP

assay_conditions_summary: pH 7.9; 303.15 K; cofactor ATP

substrate: GTP


assay_conditions_summary: pH 7.8; cofactor ATP


enzyme_display_name: thyroid-hormone transaminase

proof_lines: cosubstrate triiodothyronine, pH 7.8, 37°C

enzyme: thyroid-hormone transaminase


organism: Streptomyces griseus

substrate_name: beta-Alanine

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: beta-Alanine


organism: Ureibacillus massiliensis

proof_lines: cosubstrate beta-alanine, pH 8, temperature not specified in the publication

proof_lines: cosubstrate 2-oxoglutarate, pH 8, temperature not specified in the publication


enzyme_display_name: nucleotide diphosphokinase

organism: Streptomyces aspergilloides

substrate_name: ADP

assay_conditions_summary: pH 10; 310.15 K; cofactor ADP

enzyme: nucleotide diphosphokinase

substrate: ADP


organism: Streptomyces morookaense

substrate_name: GDP

proof_lines:
pH 10.0, 37°C, reaction with pppApp
pH 10.0, 37°C, reaction with dATP
pH 10.0, 37°C, reaction with ATP

substrate: GDP


organism: Escherichia coli

assay_conditions_summary: 295.15 K; cofactor ATP

proof_lines: pH not specified in the publication, 22°C


organism: Sus scrofa

substrate_name: UDP-GlcNAc

substrate: UDP-GlcNAc


organism: Acanthamoeba castellanii


organism: Bos taurus


organism: Glycine max


substrate_name: dolichyl phosphate

substrate: dolichyl phosphate


enzyme_display_name: GDP polyribonucleotidyltransferase

organism: vesicular stomatitis virus

enzyme: GDP polyribonucleotidyltransferase


enzyme_display_name: scymnol sulfotransferase

organism: Heterodontus portusjacksoni

proof_lines:
room temperature, pH 6.5
pH 6.5
25°C

enzyme: scymnol sulfotransferase


organism: Trygonoptera sp.


organism: Trygonorrhina fasciata


enzyme_display_name: galactosylceramide sulfotransferase

organism: Homo sapiens

substrate_name: galactosylceramide

proof_lines:
pH 6.5, 37°C
at pH 7.1 and 37°C
pH 7.0, 4°C

enzyme: galactosylceramide sulfotransferase

substrate: galactosylceramide


organism: Rattus norvegicus

proof_lines:
pH 6.2, 37°C
pH 6.2, 37°C, para isomer
pH 6.2, 37°C, meta isomer


enzyme_display_name: glycochenodeoxycholate sulfotransferase

substrate_name: lithocholate

enzyme: glycochenodeoxycholate sulfotransferase

substrate: lithocholate


organism: Mesocricetus auratus

substrate_name: glycochenodeoxycholate

proof_lines: female hamster, pH 7.0, 37°C, saturating substrate concentration

proof_lines: female hamster, pH 7.0, 37°C, physiological substrate concentration

substrate: glycochenodeoxycholate


substrate_name: 3beta-hydroxy-5beta-cholanoic acid

substrate: 3beta-hydroxy-5beta-cholanoic acid


enzyme_display_name: cortisol sulfotransferase

organism: Cavia porcellus

enzyme: cortisol sulfotransferase


organism: Gallus gallus

substrate_name: cortisol

substrate: cortisol


proof_lines:
pH 6.8, sulfotransferase I
pH 6.8, sulfotransferase III
pH 6.8, sulfotransferase I and III


enzyme_display_name: chlorophyllase

organism: Auxenochlorella protothecoides

substrate_name: chlorophyll a

enzyme: chlorophyllase

substrate: chlorophyll a


organism: Capsicum annuum

substrate_name: chlorophyll b

substrate: chlorophyll b


organism: Chenopodium album

proof_lines:
type 1 chlorophyllase
type 2 chlorophyllase


organism: Citrus unshiu


organism: Citrus x limon

substrate_name: Chlorophyll

substrate: Chlorophyll


organism: Phaeodactylum tricornutum

substrate_name: pheophytin

assay_conditions_summary: pH 8; buffer pH 8

proof_lines: immobilized enzyme in 20 mM sodium phosphate buffer pH 8

substrate: pheophytin


organism: Prunus cerasus

proof_lines:
isoform Chlase F2, at pH 7.0 and 30°C
isoform Chlase F1, at pH 7.0 and 30°C


organism: Prunus domestica

proof_lines:
isoform Chlase F1, at pH 7.0 and 30°C
isoform Chlase F2, at pH 7.0 and 30°C


organism: Prunus padus


organism: Secale cereale

substrate_name: bacteriochlorophyll a

substrate: bacteriochlorophyll a


enzyme_display_name: choline sulfotransferase

organism: Aspergillus nidulans

substrate_name: N,N-dimethylethylaminoethanol

enzyme: choline sulfotransferase

substrate: N,N-dimethylethylaminoethanol


organism: Limonium perezii

substrate_name: choline

substrate: choline


organism: Penicillium chrysogenum

proof_lines: pH 7.8, 37°C, N,N-dimethylaminoethanol as substrate

proof_lines: pH 7.8, 37°C depending on the choline concentration


enzyme_display_name: N-acetylgalactosaminoglycan deacetylase

organism: Aspergillus parasiticus

substrate_name: N-acetylated polygalactosamine

enzyme: N-acetylgalactosaminoglycan deacetylase

substrate: N-acetylated polygalactosamine


organism: Escherichia coli

substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication

substrate: Unknown


organism: Neurospora crassa

substrate_name: N-acetylgalactosamine

proof_lines:
35°C, pH 9
35°C, pH 5

substrate: N-acetylgalactosamine


organism: Staphylococcus epidermidis

substrate_name: 3-carboxyumbelliferyl acetate

substrate: 3-carboxyumbelliferyl acetate


enzyme_display_name: Serratia marcescens nuclease

organism: Serratia marcescens

substrate_name: B-Z-hybrid form DNA

proof_lines: pH 8.0, 30°C, in presence of Co(NH3)6 3+

enzyme: Serratia marcescens nuclease

substrate: B-Z-hybrid form DNA


enzyme_display_name: protein-glutamate methylesterase

organism: Bacillus subtilis

substrate_name: methylated methyl-accepting chemotaxis protein

enzyme: protein-glutamate methylesterase


organism: Rattus norvegicus

substrate_name: several methylated protein substrates

substrate: several methylated protein substrates


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: methyl-accepting chemotaxis protein

proof_lines: pH 7.0, 25°C, in absence of phosphoramidate

proof_lines: pH 7.0, 25°C, in presence of phosphoramidate

substrate: methyl-accepting chemotaxis protein


enzyme_display_name: [myosin-light-chain] phosphatase

organism: Badhamia polycephala

substrate_name: [myosin light-chain] phosphate

proof_lines: pH 7.6, temperature not specified in the publication

enzyme: [myosin-light-chain] phosphatase

substrate: [myosin light-chain] phosphate


organism: Gallus gallus

substrate_name: Phosphorylated myosin

proof_lines: at a ionic strength of 0.15 M, in the presence of 0.1 mM MgATP

substrate: Phosphorylated myosin


organism: Mammalia

substrate_name: myosin light-chain phosphate

proof_lines: smooth muscle, type 1 protein phosphatase delta and MYPT1

proof_lines: smooth muscle, type 1 protein phosphatase delta and MYPT2

proof_lines: cardiac muscle, type 1 protein phosphatase delta and MYPT2

substrate: myosin light-chain phosphate


organism: Meleagris gallopavo

substrate_name: myosin light-chain

proof_lines:
pH 7, 30°C, catalytic subunit of SMP-I
pH 7, 30°C, enzyme SMP-IV
pH 7, 30°C, SMP-I holoenzyme

substrate: myosin light-chain


organism: Acremonium sp.

substrate_name: hesperidin

proof_lines: immobilized enzyme, pH 5.0, 60°C, the change in the affinity of the enzyme to its substrate compared to the free enzyme is probably caused by structural changes introduced by the immobilization process and by the lower accessibility to the

substrate: hesperidin


organism: Acremonium sp. DSM 24697

proof_lines: isoform alphaRbetaG I, at pH 5.0 and 55°C

proof_lines: isoform alphaRbetaG II, at pH 5.0 and 40°C


enzyme_display_name: ginsenosidase type I

organism: Aspergillus niger

substrate_name: ginsenoside Rb3

proof_lines: pH 5.0, 45°C, formation of compound Mx-1

proof_lines: pH 5.0, 45°C, formation of ginsenoside Rd

enzyme: ginsenosidase type I

substrate: ginsenoside Rb3


organism: Paenibacillus mucilaginosus

substrate_name: ginsenoside Rg3

substrate: ginsenoside Rg3


enzyme_display_name: glucan 1,4-alpha-maltotetraohydrolase

organism: Roseateles saccharophilus

substrate_name: soluble starch

proof_lines: isoform maltotetraohydolase I, pH 7.5, 55°C

proof_lines: isoform maltotetraohydolase II, pH 7.5, 55°C

enzyme: glucan 1,4-alpha-maltotetraohydrolase

substrate: soluble starch


organism: Stutzerimonas stutzeri

substrate_name: oyster glycogen

proof_lines:
F1form
F1 and F2 form
F2 form

substrate: oyster glycogen


organism: Bacillus licheniformis

substrate_name: potato galactan

substrate: potato galactan


organism: Bacillus subtilis subsp. subtilis

proof_lines: pH and temperature not specified in the publication


enzyme_display_name: peptidyl-dipeptidase B

organism: Bos taurus

enzyme: peptidyl-dipeptidase B


enzyme_display_name: N-formylmethionyl-peptidase

substrate_name: N-Formyl-Met-Leu

enzyme: N-formylmethionyl-peptidase

substrate: N-Formyl-Met-Leu


enzyme_display_name: carboxypeptidase E

substrate_name: dansyl-Phe-Ala-Arg

proof_lines: pituitary brain adrenal

enzyme: carboxypeptidase E

substrate: dansyl-Phe-Ala-Arg


organism: Manduca sexta

substrate_name: dansyl-Phe-Leu-Arg

substrate: dansyl-Phe-Leu-Arg


organism: Sus scrofa

substrate_name: iodo-acetyl-Tyr-Ala-Arg

substrate: iodo-acetyl-Tyr-Ala-Arg


enzyme_display_name: deoxyribodipyrimidine endonucleosidase

organism: Micrococcus luteus

substrate_name: DNA

proof_lines:
cytosine labeled DNA
thymine labeled DNA

enzyme: deoxyribodipyrimidine endonucleosidase

substrate: DNA


organism: Paramecium bursaria Chlorella virus 1

proof_lines:
cis-syn 49-bp duplex
trans-syn 49-bp duplex
cis-syn 12-bp duplex


organism: Tequatrovirus T4

proof_lines:
thymine dimersl
arylamidated DNA


enzyme_display_name: complement factor I

organism: Homo sapiens

enzyme: complement factor I


enzyme_display_name: streptogrisin A

organism: Streptomyces griseus

enzyme: streptogrisin A


enzyme_display_name: streptogrisin B

proof_lines:
pH 8.0, 25°C, native SGPB
pH 8.0, 22°C
pH 4.0, 22°C

enzyme: streptogrisin B


enzyme_display_name: glutamyl endopeptidase II

enzyme: glutamyl endopeptidase II


enzyme_display_name: limulus clotting factor C

organism: Tachypleus tridentatus

enzyme: limulus clotting factor C


enzyme_display_name: adenain

organism: Human adenovirus sp.

proof_lines: pH 8.0, 25°C, activation by viral DNA and the 11-amino acid residue peptide from the C-terminus of adenovirus precursor protein and the viral DNA

proof_lines: pH 8.0, 25°C, in absence of any cofactor

enzyme: adenain


organism: unidentified adenovirus


enzyme_display_name: candidapepsin

organism: Candida albicans

substrate_name: albumin

enzyme: candidapepsin

substrate: albumin


organism: Candida parapsilosis


enzyme_display_name: memapsin 1

substrate_name: gamma-secretase cleavage site of notch

enzyme: memapsin 1

substrate: gamma-secretase cleavage site of notch


enzyme_display_name: peptidyl-glutaminase

organism: Niallia circulans

substrate_name: N-acetyl-L-Gln

enzyme: peptidyl-glutaminase

substrate: N-acetyl-L-Gln


enzyme_display_name: pitrilysin

organism: Acinetobacter calcoaceticus

substrate_name: Insulin

enzyme: pitrilysin

substrate: Insulin


organism: Escherichia coli

substrate_name: Substance P

substrate: Substance P


organism: Halalkalibacterium halodurans

substrate_name: dynorphin A fragment 1-13

substrate: dynorphin A fragment 1-13


enzyme_display_name: procollagen N-endopeptidase

organism: Bos taurus

substrate_name: procollagen I

enzyme: procollagen N-endopeptidase

substrate: procollagen I


organism: Gallus gallus

proof_lines:
procollagen I from human
procollagen I from chicken


substrate_name: Unknown

proof_lines:
in the presence of heparin
in the absence of heparin

substrate: Unknown


enzyme_display_name: creatinase

organism: Acinetobacter sp.

substrate_name: Creatine

enzyme: creatinase

substrate: Creatine


organism: Alcaligenes faecalis


organism: Alcaligenes sp.


organism: Paracoccus sp. WB1


organism: Pseudomonas putida


enzyme_display_name: adenosine-phosphate deaminase

organism: Meretrix lusoria

substrate_name: adenosine

enzyme: adenosine-phosphate deaminase

substrate: adenosine


organism: Porphyra crispata

assay_conditions_summary: cofactor NAD+


organism: Todarodes pacificus


organism: Turbo cornutus

substrate_name: 2',3'-Cyclic AMP

substrate: 2',3'-Cyclic AMP


enzyme_display_name: ATP deaminase

organism: Aspergillus glaucus

substrate_name: ATP

assay_conditions_summary: cofactor ATP

enzyme: ATP deaminase

substrate: ATP


organism: Desulfovibrio desulfuricans

substrate_name: ADP

substrate: ADP


organism: Microsporum audouinii


enzyme_display_name: aliphatic (R)-hydroxynitrile lyase

organism: Linum usitatissimum

enzyme: aliphatic (R)-hydroxynitrile lyase


enzyme_display_name: phosphoribosyl-AMP cyclohydrolase

organism: Methanococcus vannielii

enzyme: phosphoribosyl-AMP cyclohydrolase


organism: Methanothermobacter thermautotrophicus

proof_lines: pH 8.5, 65°C, recombinant enzyme with bound Cd2+

proof_lines: pH 8.0, 65°C, recombinant enzyme with bound Zn2+

proof_lines: pH 8.0, 65°C, recombinant enzyme with bound Cd2+


enzyme_display_name: imidazoleglycerol-phosphate dehydratase

organism: Saccharomyces cerevisiae

enzyme: imidazoleglycerol-phosphate dehydratase


organism: Triticum aestivum


organism: Arabidopsis thaliana


organism: Mycobacterium tuberculosis


organism: Neurospora crassa


enzyme_display_name: methionine gamma-lyase

substrate_name: L-methionine

enzyme: methionine gamma-lyase

substrate: L-methionine


organism: Aspergillus sp.


organism: Brevibacterium linens


organism: Citrobacter freundii

substrate_name: DL-homoserine

substrate: DL-homoserine


substrate_name: methiin

proof_lines: liposomes and polymeric vesicles-bound mutant enzyme C115H, at pH 8.0 and 30°C

proof_lines: PEGylated mutant enzyme C115H, at pH 8.0 and 30°C

proof_lines: free mutant enzyme C115H, at pH 8.0 and 30°C

substrate: methiin


organism: Citrobacter intermedius


organism: Cladosporium cladosporioides


organism: Clostridium tetani

substrate_name: L-methionine sulfoxide

substrate: L-methionine sulfoxide


organism: Pseudomonas sp.


organism: Treponema denticola


organism: Trichomonas vaginalis

substrate_name: DL-homocysteine

proof_lines:
recombinant MGL1
recombinant MGL2

substrate: DL-homocysteine


enzyme_display_name: L-3-cyanoalanine synthase

organism: Vicia sativa subsp. nigra

substrate_name: cyanide

enzyme: L-3-cyanoalanine synthase

substrate: cyanide


organism: Zea mays

substrate_name: L-cysteine

substrate: L-cysteine


organism: Zonocerus variegatus

substrate_name: NaCN

substrate: NaCN


organism: Lathyrus latifolius


organism: Lupinus sp.

substrate_name: KCN

substrate: KCN


organism: Solanum tuberosum

substrate_name: O-acetyl-L-serine

substrate: O-acetyl-L-serine


organism: Spinacia oleracea

proof_lines:
26°C, pH 9.8
vs. chloroalanine
vs. L-cysteine


organism: Entamoeba histolytica


organism: Lactococcus lactis


organism: Porphyromonas gingivalis

substrate_name: S-Benzyl-L-cysteine

substrate: S-Benzyl-L-cysteine


organism: Pseudomonas putida

substrate_name: L-ethionine

substrate: L-ethionine


enzyme_display_name: adenylate cyclase

organism: Nocardia erythropolis

enzyme: adenylate cyclase


organism: Oryctolagus cuniculus

assay_conditions_summary: 303.15 K; cofactor ATP

proof_lines: cortical adenylyl cyclase, in the presence of 7 mM Mg2+, at 30°C, pH not specified in the publication

proof_lines: medulla adenylyl cyclase, in the presence of 7 mM Mg2+, at 30°C, pH not specified in the publication

proof_lines: cortical adenylyl cyclase, in the presence of 7 mM Mn2+, at 30°C, pH not specified in the publication


organism: Paramecium tetraurelia


organism: Phycomyces blakesleeanus


organism: Mammalia

assay_conditions_summary: pH 7.4; 303.15 K; cofactor ATP

proof_lines: recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mg2+, 0.1 mM forskolin, stimulatory G protein alpha subunits guanosine 5-(gamma-thio)triphosphate

proof_lines: recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mg2+, stimulatory G protein alpha subunits guanosine 5-(gamma-thio)triphosphate


organism: Mus musculus

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

proof_lines: S49 cyc-, pH 7.4, 37°C, Mn2+, 100 microM forskolin

proof_lines: Sf9, Mn2+, pH 7.4, 37°C, 100 microM forskolin, 10 mM NaF, 10 microM AlCl3

proof_lines: Sf9, Mn2+, 100 microM forskolin, 10 mM NaF, 10 microM AlCl3


organism: Mytilus galloprovincialis

assay_conditions_summary: pH 7.4; 295.15 K; cofactor ATP


organism: Neurospora crassa


organism: Bordetella pertussis

assay_conditions_summary: pH 7.4; 298.15 K; cofactor ATP

proof_lines: pH and temperature not specified in the publication, in absence of calmodulin

proof_lines: pH and temperature not specified in the publication, in presence of calmodulin


organism: Bos taurus


organism: Canis lupus familiaris

proof_lines: recombinant cytosolic domain of type V adenylyl cyclase


organism: Cavia porcellus


organism: Escherichia coli


organism: Euglena gracilis

assay_conditions_summary: 298.15 K; cofactor ATP

proof_lines: 25°C, plasma membrane isolated from lactic acid grown cells

proof_lines: 25°C, plasma membrane isolated from glutamate/malate grown cells


organism: Gallus gallus


organism: Glutamicibacter nicotianae


organism: Synechocystis sp.

proof_lines:
in the presence of Cl-
in the presence of HCO3-
native Cya1, pH 7.5, 27°C, 50 mM NaHCO3


organism: Synechocystis sp. PCC 6803


organism: Pseudomonas aeruginosa

assay_conditions_summary: pH 8.5; 313.15 K; cofactor ATP


organism: Ruditapes philippinarum


organism: Saccharomyces cerevisiae


organism: Sus scrofa


enzyme_display_name: guanylate cyclase

organism: Arbacia punctulata

substrate_name: GTP

enzyme: guanylate cyclase

substrate: GTP


organism: Blastocladiella emersonii

proof_lines: pH 7.5, 22°C, wild-type enzyme, wild-type enzyme


proof_lines: octylglucoside solubilized enzyme, Mn2+-supported enzyme


organism: Dictyostelium discoideum

proof_lines:
soluble guanylyl cyclase sGC
22°C, pH 8.0
guanylyl cyclase GCA


organism: Dictyostelium sp.


organism: Drosophila melanogaster

proof_lines:
under anaerobic conditions
with 0.273 mM oxygen


organism: Rattus norvegicus

proof_lines: pH 8.0, 30°C, wild-type enzyme, in presence of NO-donor S-nitroso-N-acetylpenicillamine


assay_conditions_summary: pH 8; 303.15 K; cofactor ATP

proof_lines: 30°C, pH 8.0, in the presence of N-(beta-D-glucopyranosyl-N2-acetyl-S-nitroso-D,L-penicillaminamide) and YC-1


organism: Tetrahymena pyriformis


enzyme_display_name: amino-acid racemase

organism: Pseudomonas putida

substrate_name: D-ornithine

proof_lines: pH and temperature not specified in the publication, in 20% methanol

proof_lines: pH and temperature not specified in the publication, in 10% methanol

proof_lines: pH and temperature not specified in the publication, in 40% methanol

enzyme: amino-acid racemase

substrate: D-ornithine


organism: Aeromonas caviae

substrate_name: D-Ser

proof_lines: L-Lys L-Arg D-Lys

substrate: D-Ser


organism: Aplysia californica

substrate_name: L-aspartate

assay_conditions_summary: pH 8; 303.15 K; buffer 50 mM Tris-HCl

proof_lines: in 50 mM Tris-HCl buffer, pH 8.0, at 30°C

proof_lines: in 50 mM Tris-HCl buffer, pH 8.5, for Ser reactions, at 30°C

substrate: L-aspartate


organism: Geobacillus kaustophilus

substrate_name: N-succinyl-D-alanine

substrate: N-succinyl-D-alanine


organism: Mus musculus

substrate_name: L-serine

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP

proof_lines: pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction

substrate: L-serine


enzyme_display_name: ribulose-phosphate 3-epimerase

organism: Bos taurus

substrate_name: D-ribulose 5-phosphate

enzyme: ribulose-phosphate 3-epimerase

substrate: D-ribulose 5-phosphate


organism: Spinacia oleracea


enzyme_display_name: aldose 1-epimerase

organism: Aspergillus niger

substrate_name: alpha-D-glucose

enzyme: aldose 1-epimerase

substrate: alpha-D-glucose


proof_lines:
alpha-D-glucose, newborn calf
adult
embryo


organism: Capsicum frutescens


organism: Escherichia coli

substrate_name: D-xylose

substrate: D-xylose


organism: Gallus gallus


organism: Homo sapiens

proof_lines:
kidney
alpha-D-glucose, liver


organism: Lactococcus lactis

substrate_name: alpha-D-quinovose

substrate: alpha-D-quinovose


proof_lines: beta-L-arabinose, enzyme form I from kidney, enzyme form III from liver

proof_lines: alpha-D-galactose, enzyme form I from kidney

proof_lines: beta-L-arabinose, enzyme form II from liver


organism: Rhizopus stolonifer


organism: Sus scrofa

proof_lines: alpha-D-glucose, kidney, embryo and adult


enzyme_display_name: steroid Delta-isomerase

enzyme: steroid Delta-isomerase


organism: Comamonas testosteroni

proof_lines: 25°C, pH 6.5, solvent D2O, mutant enzyme D38A

proof_lines: 25°C, pH 7.5 or 8.5, solvent H2O, mutant enzyme D38A

proof_lines: 25°C, pH 8.5, solvent D2O, mutant enzyme D38A


mutation_type: truncation

proof_lines: 22°C, pH 7.4, truncated cytosolic enzyme DEALTA283-310


enzyme_display_name: L-ribulose-5-phosphate 4-epimerase

substrate_name: L-ribulose 5-phosphate

enzyme: L-ribulose-5-phosphate 4-epimerase

substrate: L-ribulose 5-phosphate


organism: Klebsiella aerogenes


enzyme_display_name: methyl-branched lipid omega-hydroxylase

organism: Mycobacterium tuberculosis

substrate_name: CYP124; 15-Methyl-palmitic acid

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

enzyme: methyl-branched lipid omega-hydroxylase

substrate: CYP124; 15-Methyl-palmitic acid


organism: Nocardia erythropolis


organism: Pseudomonas sp.


enzyme_display_name: chlorophyllase

organism: Triticum aestivum

substrate_name: H2O; Chlorophyll

proof_lines: pH 8.0, 25°C, recombinant enzyme purified by affinity chromatography

proof_lines: pH 8.0, 25°C, recombinant enzyme purified by microtiter plate method

proof_lines: final concentration of 10% v/v acetonitrile at 37°C

enzyme: chlorophyllase

substrate: H2O; Chlorophyll


organism: Clostridium sporogenes

substrate_name: H2O; L-Methionine

substrate: H2O; L-Methionine


substrate_name: ATP

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

proof_lines: isoform AC2, in 75 mM Tris/HCl, pH 7.4, at 37°C

proof_lines: isoform AC1, in 75 mM Tris/HCl, pH 7.4, at 37°C

substrate: ATP


organism: Rattus norvegicus

proof_lines: after activation of soluble AC with the stimulatory G protein alpha protein and GTP-gamma-S in the presence of Mn2+

proof_lines: after activation of soluble AC with the stimulatory G protein alpha protein and GTP-gamma-S in the presence of Mg2+


organism: Spodoptera frugiperda


enzyme_display_name: thioglucosidase

organism: Raphanus sativus

substrate_name: H2O; Sinigrin

proof_lines:
in absence of ascorbate
in presence of 0.5 mM ascorbate

enzyme: thioglucosidase

substrate: H2O; Sinigrin


enzyme_display_name: L-ascorbate peroxidase

organism: Lactuca sativa

substrate_name: H2O2; L-Ascorbate

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

enzyme: L-ascorbate peroxidase

substrate: H2O2; L-Ascorbate


organism: Spinacia oleracea


organism: Ovis aries


enzyme_display_name: catechol O-methyltransferase

substrate_name: Rv0187; S-Adenosyl-L-methionine

enzyme: catechol O-methyltransferase

substrate: Rv0187; S-Adenosyl-L-methionine


organism: Streptococcus pyogenes

substrate_name: D-Ribulose 5-phosphate

substrate: D-Ribulose 5-phosphate


organism: Lactococcus lactis subsp. lactis

substrate_name: alpha-D-Glucose

substrate: alpha-D-Glucose


enzyme_display_name: D-sedoheptulose-7-phosphate isomerase

substrate_name: HddA; GmhA

enzyme: D-sedoheptulose-7-phosphate isomerase

substrate: HddA; GmhA


organism: Oryza sativa subsp. japonica

substrate_name: ascorbate

data_origin: experimental

substrate: ascorbate


enzyme_display_name: demethylmacrocin O-methyltransferase

organism: Streptomyces fradiae

substrate_name: demethylmacrocin

enzyme: demethylmacrocin O-methyltransferase

substrate: demethylmacrocin


organism: Aquifex aeolicus (strain VF5)

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


enzyme_display_name: cephaeline 6'-O-methyltransferase

organism: Carapichea ipecacuanha

substrate_name: cephaeline

enzyme: cephaeline 6'-O-methyltransferase

substrate: cephaeline


enzyme_display_name: D-alanine 2-hydroxymethyltransferase

organism: Aminobacter sp

substrate_name: alpha-methyl-L-serine

enzyme: D-alanine 2-hydroxymethyltransferase

substrate: alpha-methyl-L-serine


organism: Ensifer sp


organism: Paracoccus sp


enzyme_display_name: 2-hydroxy-3-oxoadipate synthase

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

enzyme: 2-hydroxy-3-oxoadipate synthase


organism: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

substrate_name: alpha-ketoglutarate

substrate: alpha-ketoglutarate


enzyme_display_name: very-long-chain ceramide synthase

organism: Homo sapiens

substrate_name: sphinganine

assay_conditions_summary: cofactor CoA

enzyme: very-long-chain ceramide synthase

substrate: sphinganine


organism: Cenchrus americanus


organism: Chlamydomonas sp.


enzyme_display_name: aliphatic (R)-hydroxynitrile lyase

organism: Linum usitatissimum

substrate_name: acetone cyanohydrin

enzyme: aliphatic (R)-hydroxynitrile lyase

substrate: acetone cyanohydrin


enzyme_display_name: glutamate racemase

organism: Bacillus anthracis

substrate_name: L-glutamate

assay_conditions_summary: pH 8.2; 298.15 K; cofactor NAD+

proof_lines: mutant R214A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol

proof_lines: mutant R214A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0

enzyme: glutamate racemase

substrate: L-glutamate


substrate_name: D-glutamate

assay_conditions_summary: pH 9.2; 298.15 K; cofactor NAD+

proof_lines: mutant Q86A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.

proof_lines: mutant Q86A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol

substrate: D-glutamate


proof_lines: mutant P99A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.

proof_lines: mutant P99A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol


proof_lines: mutant K106A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol

proof_lines: mutant K106A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0

proof_lines: RacE2 mutant enzyme K106A, pH and temperature not specified in the publication


organism: Burkholderia cenocepacia


enzyme_display_name: adenylate cyclase

organism: Alternaria solani

substrate_name: ATP

enzyme: adenylate cyclase

substrate: ATP


organism: Anabaena sp.

proof_lines: recombinant CyaB1-maltose binding protein fusion


assay_conditions_summary: pH 7.2; 303.15 K; cofactor ATP

proof_lines: forward reaction, 0.1 microM free Ca2+, pH 7.2, 30°C


organism: Bombyx mori


enzyme_display_name: 4-chlorobenzoyl-CoA dehalogenase

organism: Pseudomonas sp.

source_db: oed

enzyme: 4-chlorobenzoyl-CoA dehalogenase

source: oed


enzyme_display_name: imidazoleglycerol-phosphate dehydratase

organism: Arabidopsis thaliana

substrate_name: Unknown

proof_lines: 30°C, pH not specified in the publication

enzyme: imidazoleglycerol-phosphate dehydratase

substrate: Unknown


substrate_name: S-Adenosyl-L-methionine

substrate: S-Adenosyl-L-methionine


organism: Mycobacterium tuberculosis

substrate_name: Glucose 6-phosphate; GDPglucose

substrate: Glucose 6-phosphate; GDPglucose


organism: Streptomyces venezuelae

substrate_name: glucose 6-phosphate

source_license: CC BY 4.0; CC BY-NC-ND 4.0

assay_conditions_summary: cofactor ADP

proof_lines: with 2.5 mM ADP-glucose as cosubstrate, pH and temperature not specified in the publication

proof_lines: with 0.5 mM GDP-glucose as cosubstrate, pH and temperature not specified in the publication

source_databases_merged: brenda skid

substrate: glucose 6-phosphate


organism: Pseudomonas reinekei

assay_conditions_summary: pH 7.5; 298.15 K; cofactor CoA


substrate_name: 4-Chlorobenzoyl-CoA; H2O

substrate: 4-Chlorobenzoyl-CoA; H2O


proof_lines: single turnover, mutant H90Q, pH 7.5, 25°C

proof_lines: multiple turnovers, mutant H90Q, pH 7.5, 25°C


proof_lines: multiple turnovers, wild-type enzyme, pH 7.5, 25°C

proof_lines: single turnover, wild-type enzyme, pH 7.5, 25°C


substrate_name: H2O; 4-Chlorobenzoyl-CoA

substrate: H2O; 4-Chlorobenzoyl-CoA


enzyme_display_name: 5-formyltetrahydrofolate cyclo-ligase

substrate_name: ATP; 5-Formyltetrahydrofolyl-glutamate

enzyme: 5-formyltetrahydrofolate cyclo-ligase

substrate: ATP; 5-Formyltetrahydrofolyl-glutamate


organism: Mus musculus

proof_lines:
pH 6.0, 28°C, recombinant enzyme
recombinant enzyme


organism: Mycoplasma pneumoniae

substrate_name: ATP; 5-Formyltetrahydrofolate

substrate: ATP; 5-Formyltetrahydrofolate


assay_conditions_summary: pH 6; 310.15 K; cofactor ATP


source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0


organism: Mycoplasmoides pneumoniae

proof_lines: mutant enzyme K120A, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C


proof_lines: wild type enzyme, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C


organism: Saccharomyces cerevisiae


organism: Unknown


substrate_name: L-Vinylglycine

substrate: L-Vinylglycine


assay_conditions_summary: cofactor ATP


proof_lines:
below, mutant F64P, pH 7.5, 25°C
mutant F64P, pH 7.5, 25°C


proof_lines:
wild-type enzyme, pH 7.5, 25°C
pH 7.5, 25°C, wild-type enzyme
about, wild-type enzyme, pH 7.5, 25°C


proof_lines: mutant enzyme D81A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: mutant enzyme K120A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: mutant enzyme D151A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: mutant enzyme W153A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C

proof_lines: mutant enzyme W153A, Km above 5 mM, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: mutant enzyme R125A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C

proof_lines: mutant enzyme R125A, Km above 5 mM, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: mutant enzyme K3D/D151K, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: mutant enzyme K3A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C

proof_lines: mutant enzyme K3A, Km above 5 mM, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: mutant enzyme R7A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C

proof_lines: mutant enzyme R7A, Km above 5 mM, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: wild type enzyme, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C


proof_lines: mutant enzyme Y152A, in 100 mM MES, pH 6.0, at 37°C


substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


proof_lines: mutant enzyme Y152F, in 100 mM MES, pH 6.0, at 37°C


proof_lines: wild type enzyme, in 100 mM MES, pH 6.0, at 37°C


proof_lines: mutant enzyme Y151A, in 100 mM MES, pH 6.0, at 37°C


organism: Solanum lycopersicum

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


enzyme_display_name: flavone synthase I

organism: Oryza sativa

substrate_name: S-naringenin

assay_conditions_summary: pH 8; 310.15 K; buffer 10 mM Tris/HCl

proof_lines: 37°C, 50 microM FeSO4, 100 mg/ml catalase, 160 microM oxoglutarate, 1 mM ascorbate, 60 microM substrate in 10 mM Tris/HCl buffer, pH 8.0

enzyme: flavone synthase I

substrate: S-naringenin


organism: Petroselinum crispum


enzyme_display_name: tagaturonate reductase

organism: Escherichia coli

substrate_name: NADH

enzyme: tagaturonate reductase

substrate: NADH


organism: Arthrobacter sp.

assay_conditions_summary: pH 7.5; 303.15 K; cofactor CoA


proof_lines:
single turnover
multiple turnovers


organism: Cucurbita maxima


organism: Cucurbita pepo


organism: Dianthus caryophyllus


organism: Diospyros kaki


organism: Vigna radiata


organism: Vigna radiata var. radiata

proof_lines: 8 h after chilling in the presence of 1,4-diazabicyclo(2,2,2) octane


organism: Malus domestica

substrate_name: (S,S)-S-adenosylmethionine

substrate: (S,S)-S-adenosylmethionine


organism: Malus sylvestris


organism: Penicillium citrinum


proof_lines:
at pH 8.5 and 30°C
Km increases from pH 7.5 to 9.5


organism: Homo sapiens

proof_lines:
cytosolic enzyme
mitochondrial enzyme
at 37°C in 100 mM MES, pH 6.0


organism: Lacticaseibacillus casei


organism: Mycoplasmoides pneumoniae


organism: Oryctolagus cuniculus

proof_lines: Km-value is constant over the pH-range 5.0-7.5


organism: Ovis aries


organism: Rattus norvegicus


organism: Saccharomyces cerevisiae

assay_conditions_summary: pH 7; 303.15 K; cofactor ATP


organism: Pichia pastoris

substrate_name: S-Methyl-L-methionine

substrate: S-Methyl-L-methionine


enzyme_display_name: prolyl oligopeptidase

substrate_name: Ala-Pro (Dipeptidyl peptidase activity)

enzyme: prolyl oligopeptidase

substrate: Ala-Pro (Dipeptidyl peptidase activity)


enzyme_display_name: dCTP deaminase

enzyme: dCTP deaminase


enzyme_display_name: prenyl-diphosphate phosphatase

organism: Mus musculus

enzyme: prenyl-diphosphate phosphatase


organism: Escherichia coli O157:H7

substrate_name: histidinol phosphate (in the presence of Mg(2+))


enzyme_display_name: aspartate racemase

organism: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)

substrate_name: L-aspartate

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

enzyme: aspartate racemase

substrate: L-aspartate


organism: Plagiochasma appendiculatum

substrate_name: naringenin

substrate: naringenin


enzyme_display_name: thiosulfate dehydrogenase (quinone)

organism: Acidianus ambivalens

substrate_name: thiosulfate

enzyme: thiosulfate dehydrogenase (quinone)

substrate: thiosulfate


enzyme_display_name: type II protein arginine methyltransferase

proof_lines: isoform PRMT5 in complex with MEP50, presence of 0.001 mM histone H4 peptide, 22°C, pH not specified in the publication

proof_lines: isoform PRMT5, presence of 0.001 mM histone H4 peptide, 22°C, pH not specified in the publication


enzyme_display_name: diaminobutyrate acetyltransferase

organism: Methylophaga alcalica

enzyme: diaminobutyrate acetyltransferase


organism: Methylophaga thalassica


organism: Paenibacillus lautus

assay_conditions_summary: pH 7.4; 303.15 K; cofactor CoA


organism: Trifolium pratense

substrate_name: L-tyrosine

assay_conditions_summary: pH 8; 298.15 K; cofactor CoA

substrate: L-tyrosine


enzyme_display_name: cullin-RING-type E3 NEDD8 transferase

organism: Homo sapiens

substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication

enzyme: cullin-RING-type E3 NEDD8 transferase

substrate: Unknown


organism: Streptomyces venezuelae


proof_lines: pH 8.0, 29°C, addition of the DesIII protein, cosubstrate: 1.0 mM dTDP-3-amino-4,6-dideoxy-alpha-D-glucopyranose

proof_lines: pH 8.0, 29°C, addition of the DesIII protein, cosubstrate: 9.1 mM 10-deoxymethynolide

proof_lines: pH 8.0, 29°C, cosubstrate: 9.1 mM 10-deoxymethynolide


enzyme_display_name: ceramide glucosyltransferase

enzyme: ceramide glucosyltransferase


enzyme_display_name: fructan beta-(2,1)-fructosidase

organism: Lolium perenne

enzyme: fructan beta-(2,1)-fructosidase


enzyme_display_name: 6-aminohexanoate-oligomer endohydrolase

organism: Agromyces sp.

substrate_name: 6-aminohexanoate cyclic oligomer

proof_lines: pH 7.3, 60°C, mutant enzyme D122G/H130Y/D36A/E236Q

enzyme: 6-aminohexanoate-oligomer endohydrolase

substrate: 6-aminohexanoate cyclic oligomer


organism: Escherichia coli

substrate_name: dCTP

proof_lines: pH 6.8, 37°C, recombinant wild-type enzyme

substrate: dCTP


organism: Paramecium bursaria Chlorella virus 1

proof_lines: pH 9.5, 42°C, in presence of 0.1 mM dCTP


enzyme_display_name: o-pyrocatechuate decarboxylase

organism: Fusarium odoratissimum NRRL 54006

substrate_name: catechol

enzyme: o-pyrocatechuate decarboxylase

substrate: catechol


organism: Arabidopsis thaliana


substrate_name: L-cystine

substrate: L-cystine


organism: Tequatrovirus T4

substrate_name: deoxycytidylate

substrate: deoxycytidylate


enzyme_display_name: formate dehydrogenase (cytochrome c-553)

organism: Nitratidesulfovibrio vulgaris

substrate_name: formate

substrate: formate


enzyme: formate dehydrogenase (cytochrome c-553)


enzyme_display_name: sulfiredoxin

organism: Saccharomyces cerevisiae

substrate_name: gamma-S-ATP

enzyme: sulfiredoxin

substrate: gamma-S-ATP


enzyme_display_name: retroviral ribonuclease H

organism: Human immunodeficiency virus 1

substrate_name: RNA-DNA hybrid

proof_lines:
wild type enzyme, at pH 7.8 and 37°C
37°C, wild-type p66/p51

enzyme: retroviral ribonuclease H

substrate: RNA-DNA hybrid


enzyme_display_name: plant seed peroxygenase

organism: Lathyrus oleraceus

substrate_name: linoleic acid hydroperoxide

proof_lines: partially purified enzyme, at pH 7.2 and 25°C

proof_lines: partially purified enzyme, using linoleic acid hydroperoxide as cosubstrate, at pH 7.2 and 25°C

enzyme: plant seed peroxygenase

substrate: linoleic acid hydroperoxide


organism: Solanum lycopersicum

substrate_name: linoleic acid

substrate: linoleic acid


organism: Zea mays


enzyme_display_name: nicotinate dehydrogenase

organism: Clostridium sp.

substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

enzyme: nicotinate dehydrogenase

substrate: NADP+


organism: Neobacillus niacini

substrate_name: nicotinate

substrate: nicotinate


organism: Pseudomonas fluorescens


organism: Nitratidesulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / CCUG 34227 / NCIMB 8303 / VKM B-1760 / Hildenborough)

substrate_name: formate (at pH 9.5 and 30 degrees Celsius)

proof_lines: Measurements have been done with the heterotrimer complex. kcat is 262 sec(-1) with formate as substrate (at pH 7.6).


organism: Acidianus ambivalens

substrate_name: thiosulfate (with ferrocyanide as electron acceptor)

proof_lines: KM values measured using the TQO doxDA complex.


substrate: thiosulfate (with ferrocyanide as electron acceptor)


substrate_name: peroxiredoxin-(S-hydroxy-S-oxocysteine) (in the presence of 1 mM of ATP)

proof_lines: kcat is 0.66 min(-1) with peroxiredoxin-(S-hydroxy-S-oxocysteine) as substrate (in the presence of 1 mM of ATP) and 0.025 min(-1) with ATP as substrate (in the presence of 40 uM of peroxiredoxin-(S-hydroxy-S-oxocysteine)).


organism: Streptomyces niveus

substrate_name: decarbamoylnovobiocin

proof_lines: kcat is 4.1 min(-1) with decarbamoylnovobiocin as substrate.

substrate: decarbamoylnovobiocin


organism: Streptomyces anulatus

proof_lines: kcat is 0.19 min(-1) with 4-MHA as substrate. kcat is 0.44 min(-1) with 4-MHB as substrate. kcat is 0.32 min(-1) with 3-HA as substrate.


organism: Agromyces sp. (strain KY5R)

substrate_name: Ahx cyclic-oligomer

proof_lines: kcat is 11.1 sec(-1) with Ahx cyclic-oligomer as substrate.

substrate: Ahx cyclic-oligomer


organism: Kocuria sp. (strain KY2)

proof_lines: kcat is 11.7 sec(-1) with Ahx cyclic-oligomer as substrate.


organism: Paenarthrobacter ureafaciens

proof_lines: kcat is 6.5 sec(-1) with Ahx cyclic-oligomer as substrate.


organism: Escherichia coli (strain K12)

substrate_name: Unknown


organism: Pusillimonas sp. (ex Stolz et al. 2005)

proof_lines: recombinant enzyme, with phenazine methosulfate, pH 7.0, 30°C


organism: Acidithiobacillus ferrooxidans

substrate_name: thiosulfate

proof_lines:
recombinant enzyme, at pH 2.5 and 50°C
native enzyme, at pH 2.5 and 70°C

substrate: thiosulfate


organism: Desulfovibrio desulfuricans

substrate_name: ferri-Ssc

substrate: ferri-Ssc


organism: Arabidopsis thaliana

substrate_name: peroxiredoxin-(S-hydroxy-S-oxocysteine)

proof_lines: pH 7.5, 30°C, 1 mM ATP, 0.03 mM Srx, saturating substrate concentration

proof_lines: pH 7.5, 30°C, saturating ATP, 0.03 mM Srx, 0.04 mM substrate

substrate: peroxiredoxin-(S-hydroxy-S-oxocysteine)


organism: Homo sapiens

assay_conditions_summary: 303.15 K; cofactor ATP


organism: Tequatrovirus T4

substrate_name: tetrahydrofolate

substrate: tetrahydrofolate


organism: Avena sativa

substrate_name: p-Coumaroyl-CoA

assay_conditions_summary: pH 7

proof_lines: pH 7, temperature not specified in the publication

substrate: p-Coumaroyl-CoA


organism: Methylophaga alcalica

assay_conditions_summary: pH 9.5; 298.15 K; cofactor CoA


organism: Methylophaga thalassica

substrate_name: acetyl-CoA

substrate: acetyl-CoA


organism: Methylotuvimicrobium alcaliphilum


organism: Sus scrofa

substrate_name: UDP-glucose

substrate: UDP-glucose


organism: Gallus gallus


proof_lines:
in absence of c-Fos
in presence of c-Fos
substrate is fluorescent


organism: Raphanus sativus


organism: Rattus norvegicus


substrate_name: oligosaccharide substrate derived from chondroitin 6-sulfate

proof_lines:
liver enzyme
recombinant enzyme


organism: Cichorium intybus


organism: Helianthus tuberosus


organism: Triticum aestivum

proof_lines:
isoenzyme1-FeH w1
isoenzyme1-FeH w2


organism: Streptomyces anulatus


organism: Streptomyces parvulus

substrate_name: 3-hydroxyanthranilic acid

proof_lines: at 30°C, pH not specified in the publication

substrate: 3-hydroxyanthranilic acid


enzyme_display_name: ginsenosidase type IV

organism: Aspergillus sp.

substrate_name: ginsenoside Re

enzyme: ginsenosidase type IV

substrate: ginsenoside Re


organism: Gordonia terrae


enzyme_display_name: scytalidopepsin A

organism: Scytalidium lignicola

substrate_name: Benzyloxycarbonyl-Ala-Phe-Leu-Ala

enzyme: scytalidopepsin A

substrate: Benzyloxycarbonyl-Ala-Phe-Leu-Ala


enzyme_display_name: dCTP deaminase

organism: Bacillus subtilis

substrate_name: dCTP

enzyme: dCTP deaminase

substrate: dCTP


organism: Methanocaldococcus jannaschii

substrate: Unknown


organism: Arthrobacter sp.

proof_lines: pH and temperature not specified in the publication


organism: Pseudomonas sp.


organism: Aspergillus niger


organism: Aspergillus oryzae


organism: Cutaneotrichosporon cutaneum


organism: Klebsiella pneumoniae


organism: Avena sativa


enzyme_display_name: 20-O-multi-glycoside ginsenosidase

substrate_name: ginsenoside Rb1

proof_lines:
free enzyme, at pH 5.5 and 40°C
immobilized enzyme, at pH 5.5 and 40°C

enzyme: 20-O-multi-glycoside ginsenosidase

substrate: ginsenoside Rb1


organism: Bifidobacterium breve

substrate_name: ginsenoside Rd

substrate: ginsenoside Rd


organism: Bifidobacterium longum

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

proof_lines:
at pH 7.2 and 37°C
at pH 6.0 and 37°C

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Lissachatina fulica


organism: Paenibacillus polymyxa


organism: Pseudarthrobacter chlorophenolicus


organism: Thermotoga petrophila


organism: Crithidia fasciculata

substrate_name: R'C(R)SH; t-Butylhydroperoxide

substrate: R'C(R)SH; t-Butylhydroperoxide


organism: Fasciola hepatica

smiles: OO


substrate_name: cumene hydroperoxide

substrate: cumene hydroperoxide


substrate_name: H2O2; Reduced thioredoxin

substrate: H2O2; Reduced thioredoxin


organism: Leishmania donovani


organism: Leishmania infantum


organism: Trypanosoma brucei brucei

substrate_name: t-Butylhydroperoxide

smiles: CC(C)(C)OO

substrate: t-Butylhydroperoxide


organism: Trypanosoma cruzi


enzyme_display_name: flavonoid 3'-monooxygenase

organism: Arabidopsis thaliana

substrate_name: dihydrokaempferol

enzyme: flavonoid 3'-monooxygenase

substrate: dihydrokaempferol


substrate_name: kaempferol

substrate: kaempferol


organism: Oryza sativa Japonica Group

proof_lines:
pH 7.5, 30°C, enzyme from white rice
pH 7.5, 30°C, enzyme from black rice


organism: Tagetes erecta

substrate_name: naringenin

substrate: naringenin


substrate_name: apigenin

substrate: apigenin


organism: Mycobacterium tuberculosis

proof_lines:
pH 7.5, 25°C
Raw SKiD Env: Temp=25°C, pH=7.5


organism: Mycolicibacterium smegmatis

proof_lines: mutant Y87W, pH 7.4, temperature not specified in the publication


proof_lines: mutant W87Y, pH 7.4, temperature not specified in the publication


proof_lines: wild-type, pH 7.4, temperature not specified in the publication


organism: Camellia sinensis


organism: Dahlia pinnata

proof_lines:
allele 2, pH 7.5, 30°C
allele 1, pH 7.5, 30°C


organism: Fragaria x ananassa


enzyme_display_name: phosphooxymethylpyrimidine kinase

organism: Escherichia coli

proof_lines: pH 7.5, temperature not specified in the publication, recombinant enzyme ThiD

enzyme: phosphooxymethylpyrimidine kinase


organism: Sulfurimonas denitrificans

proof_lines: pH 7.5, temperature not specified in the publication, recombinant enzyme ThiD2


enzyme_display_name: phosphatidylglycerophosphatase

enzyme: phosphatidylglycerophosphatase


organism: Bacteroides ovatus

substrate_name: Candida mannan

substrate: Candida mannan


organism: Bacteroides thetaiotaomicron


organism: Dyadobacter fermentans


enzyme_display_name: DNA-deoxyinosine glycosylase

organism: Pedobacter heparinus

substrate_name: uracil-containing double-stranded DNA

proof_lines:
G/U pairing, pH 7.5, 37°C
T/hypoxanthine pairing, pH 7.5, 37°C
A/U pairing, pH 7.5, 37°C

enzyme: DNA-deoxyinosine glycosylase

substrate: uracil-containing double-stranded DNA


enzyme_display_name: peptidase Do

organism: Homo sapiens

substrate_name: beta-casein

enzyme: peptidase Do

substrate: beta-casein


proof_lines: pH not specified in the publication, at 37°C


organism: Plasmodium falciparum

proof_lines: pH 7.0, temperature not specified in the publication


organism: Entamoeba histolytica

proof_lines: pH 5.5, 37°C, recombinant mutant D46A cytoplasmic catalytic domain, no metal added

proof_lines: pH 5.5, 37°C, recombinant mutant D46A cytoplasmic catalytic domain, metal added


enzyme_display_name: nitrate reductase (quinone)

substrate_name: nitrate

enzyme: nitrate reductase (quinone)

substrate: nitrate


enzyme_display_name: adenosine tuberculosinyltransferase

substrate_name: tuberculosinyl diphosphate

enzyme: adenosine tuberculosinyltransferase

substrate: tuberculosinyl diphosphate


organism: Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4)

proof_lines: Characterized with purified enzyme corresponding to a dimer of NarG and NarH.


enzyme_display_name: (E3-independent) E2 ubiquitin-conjugating enzyme

substrate_name: beta-catenin-monoubiquitin

proof_lines: Measured for E2-E3 complex composed of neddylated UBE2R1 and the CRL2(FEM1C) complex, using an 'Arg-48' donor ubiquitin.

substrate: beta-catenin-monoubiquitin


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: tuberculosinyl diphosphate (at 45 degrees Celsius and pH 7)

proof_lines: kcat is 0.031 min(-1) with tuberculosinyl diphosphate as substrate.


enzyme_display_name: GTP 3',8-cyclase

proof_lines: kcat is 0.092 min(-1) for (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.

enzyme: GTP 3',8-cyclase


organism: Staphylococcus aureus (strain NCTC 8325 / PS 47)

substrate_name: GTP

substrate: GTP


enzyme_display_name: orcinol 2-monooxygenase

organism: Pseudomonas putida

substrate_name: orcinol

assay_conditions_summary: cofactor NADPH

proof_lines:
crystalline enzyme
cofactor: NADH
cofactor: 3-acetylpyridine NADH

enzyme: orcinol 2-monooxygenase

substrate: orcinol


organism: Citrus sinensis


organism: Columnea hybrida


organism: Torenia sp.


enzyme_display_name: asparagusate reductase

organism: Asparagus officinalis

substrate_name: potassium ferricyanide

proof_lines:
enzyme I
enzyme II
enzyme I and II

enzyme: asparagusate reductase

substrate: potassium ferricyanide


organism: Escherichia coli

substrate_name: ATP

assay_conditions_summary: pH 8; 298.15 K; cofactor ATP

substrate: ATP


organism: Oryctolagus cuniculus

substrate_name: [histone H2A]-L-lysine

proof_lines:
E2 enzyme of 20 kDa, pH 7.5, 37°C
pH 7.0, 37°C, Vmax, 0.06 microM
pH 7.0, 37°C, Vmax, 0.12 microM

enzyme: (E3-independent) E2 ubiquitin-conjugating enzyme

substrate: [histone H2A]-L-lysine


proof_lines:
pH 7
pH 7.0
pH 7.0, competitive inhibition constant


organism: Escherichia coli K-12


organism: Mycobacterium tuberculosis

substrate_name: cholesterol

proof_lines: pH and temperature not specified in the publication

proof_lines: apparent value, Km above 1.2 mM, in 0.1 M potassium phosphate at pH 7.0, temperature not specified in the publication

proof_lines: apparent value, in 0.1 M potassium phosphate at pH 7.0, temperature not specified in the publication

substrate: cholesterol


organism: Bos taurus

substrate_name: poly(dAdT)

substrate: poly(dAdT)


organism: Bacillus licheniformis

substrate_name: phosphatidylglycerophosphate

substrate: phosphatidylglycerophosphate


organism: Rattus sp.


organism: Saccharomyces cerevisiae


enzyme_display_name: venombin AB

organism: Bitis gabonica

substrate_name: Tosyl-Gly-Pro-Arg 4-nitroanilide

enzyme: venombin AB

substrate: Tosyl-Gly-Pro-Arg 4-nitroanilide


organism: Vigna radiata

substrate_name: dipalmitoyl sn-glycero 3-phosphate

substrate: dipalmitoyl sn-glycero 3-phosphate


organism: Homo sapiens


organism: Streptococcus pneumoniae

substrate_name: CSP-1 FRET peptide

substrate: CSP-1 FRET peptide


enzyme_display_name: pantothenase

organism: Pseudomonas fluorescens

substrate_name: pantothenic acid

proof_lines: 10 mM Tris/10 mM citric acid(KOH), pH 7.5

enzyme: pantothenase

substrate: pantothenic acid


enzyme_display_name: Ste24 endopeptidase

organism: Saccharomyces mikatae

substrate_name: Abz-KSKTKC(farnesyl)VIKD

proof_lines: in the absence of insulin B chain, at pH 7.5 and 30°C

proof_lines: in the presence of 0.02 mM insulin B chain, at pH 7.5 and 30°C

enzyme: Ste24 endopeptidase

substrate: Abz-KSKTKC(farnesyl)VIKD


organism: Escherichia coli (strain K12)

substrate_name: RseA-YQF

substrate: RseA-YQF


enzyme_display_name: prolyl oligopeptidase

substrate_name: Gly-Pro (Prolyl endopeptidase activity)

enzyme: prolyl oligopeptidase

substrate: Gly-Pro (Prolyl endopeptidase activity)


organism: Dictyostelium discoideum

substrate_name: carbobenzoxy-Gly-Pro-p-nitroanilide (at 37 degrees Celsius)

verification_status: verified

column_label: Table I, page 2738, row Z-Gly-Pro-pNA / Dictyostelium DpoA, Km column: 115 +/- 13 uM. Methods page 2742 gives the assay conditions. Figure 3, pages 2736-2737, prints the full dpoA cDNA and predicted 760-aa product from wild-type Ax2 cells.

normalized_value_unit_evidence: Km normalized_value: 0.115 mM from the printed 115 uM value; it exactly matches the current source-aligned preimage.

raw_value_unit_evidence: Table I prints Km = 115 +/- 13 uM for Z-Gly-Pro-pNA with Dictyostelium DpoA.

row_label: Z-Gly-Pro-pNA / Dictyostelium DpoA

table_label: Table I

status: verified


organism: Galerina marginata (strain CBS 339.88)

substrate_name: the full alpha-amanitin 35mer proprotein

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


substrate_name: Tosyl-L-arginine methyl ester (TAME)

sequence: VVGGAECKIDGHRCLALLY

wild_type_sequence: VVGGAECKIDGHRCLALLY

substrate: Tosyl-L-arginine methyl ester (TAME)


enzyme_display_name: N-methylhydantoinase (ATP-hydrolyzing)

organism: Pseudomonas putida

substrate_name: dihydrouracil

enzyme: N-methylhydantoinase (ATP-hydrolyzing)

substrate: dihydrouracil


organism: Entamoeba histolytica

assay_conditions_summary: pH 5.5; 310.15 K; buffer 50 mM acetate

proof_lines: in the absence of metal ions, in 50 mM acetate buffer at pH 5.5 and 37°C

proof_lines: in the presence of 5 mM Mn2+, in 50 mM acetate buffer at pH 5.5 and 37°C


proof_lines:
presence of 5 mM Mg2+, pH 6.5, 30°C
pH 6.5, 30°C


organism: Trypanosoma sp.


organism: Staphylococcus aureus


organism: Unknown

substrate_name: GDPmannose

substrate: GDPmannose


enzyme_display_name: phosphoribosylanthranilate isomerase

enzyme: phosphoribosylanthranilate isomerase


organism: Thermotoga maritima

proof_lines: pH 7.5, 25°C, D130P, mutant form of imidazole glycerol phosphate synthase


proof_lines: pH 7.5, 25°C, D130T, mutant form of imidazole glycerol phosphate synthase


proof_lines: pH 7.5, 25°C, D127K, mutant form of EC 5.3.1.6


proof_lines: pH 7.5, 25°C, D127G, mutant form of EC 5.3.1.6


proof_lines: mutant enzyme D130V, in 50 mM HEPES, pH 7.5, at 25°C

proof_lines: pH 7.5, 25°C, D130V, mutant form of imidazole glycerol phosphate synthase


proof_lines: mutant enzyme D127V, in 50 mM HEPES, pH 7.5, at 25°C

proof_lines: pH 7.5, 25°C, D127V, mutant form of EC 5.3.1.6


proof_lines: mutant enzyme D127V/D173V, in 50 mM HEPES, pH 7.5, at 25°C


proof_lines: mutant enzyme D173V, in 50 mM HEPES, pH 7.5, at 25°C


proof_lines: mutant enzyme D127V/D169V, in 50 mM HEPES, pH 7.5, at 25°C


proof_lines: wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C


proof_lines: mutant enzyme D130V/D176V, in 50 mM HEPES, pH 7.5, at 25°C


proof_lines: mutant enzyme D169V, in 50 mM HEPES, pH 7.5, at 25°C


proof_lines: pH 7.5, 25°C, D127V/T164H, mutant form of EC 5.3.1.6


proof_lines: pH 7.5, 25°C, D127T, mutant form of EC 5.3.1.6


proof_lines: pH 7.5, 25°C, D127F, mutant form of EC 5.3.1.6


organism: Phaseolus lunatus

substrate_name: trans-zeatin

substrate: trans-zeatin


enzyme_display_name: streptomycin 6-kinase

organism: Escherichia coli

substrate_name: ATP

assay_conditions_summary: pH 7.5; 298.15 K; cofactor ATP

enzyme: streptomycin 6-kinase


organism: Streptomyces griseus


organism: Cucumis sativus

substrate_name: diphosphate

proof_lines:
pH 7.2, 30°C
phosphate in form of Mg-diphosphate

substrate: diphosphate


substrate_name: alpha-D-glucose 1-phosphate

substrate: alpha-D-glucose 1-phosphate


organism: Homo sapiens

substrate_name: UDP-galactose

proof_lines:
healthy persons, pH 7.8, 37°C
galactosemic individuals, pH 7.8, 37°C
healthy individuals, pH 7.8, 37°C

substrate: UDP-galactose


organism: Bos taurus


enzyme_display_name: itaconyl-CoA hydratase

organism: Pseudomonas aeruginosa

assay_conditions_summary: pH 7.5; 318.15 K; cofactor CoA

enzyme: itaconyl-CoA hydratase


organism: Yersinia pestis

substrate_name: (S)-citramalyl-CoA

substrate: (S)-citramalyl-CoA


organism: Pseudomonas fluorescens

substrate_name: Salicylaldehyde

substrate: Salicylaldehyde


organism: Rhodococcus opacus

substrate_name: trans-o-hydroxybenzylidenepyruvate

proof_lines: pH and temperature not specified in the publication

substrate: trans-o-hydroxybenzylidenepyruvate


organism: Sphingobium xenophagum


organism: Sphingomonas paucimobilis

proof_lines:
30°C, pH 8.1, enzyme form fHBP HA A
30°C, pH 8.1, enzyme form fHBP HA B


organism: Ogataea henricii


organism: Saccharomyces cerevisiae

proof_lines: pH 7.5, 25°C, recombinant xPRAI, expressed in E. coli

proof_lines: pH 7.5, 25°C, recombinant xPRAI, expressed in Escherichia coli


proof_lines:
pH 7.5, 45°C, dimer
pH 7.5, 80°C, dimer
pH 7.5, 60°C, dimer


enzyme_display_name: phosphatidate cytidylyltransferase

substrate_name: phosphatidate

enzyme: phosphatidate cytidylyltransferase

substrate: phosphatidate


organism: Bacillus subtilis

substrate_name: CTP; Phosphatidate

substrate: CTP; Phosphatidate


proof_lines:
pH 8.0, 22°C
pH 8.0, 22°C, recombinant CDS2


proof_lines:
pH 8.0, 22°C
pH 8.0, 22°C, recombinant CDS1


organism: Rattus norvegicus


proof_lines: with NBD-phosphatidate, pH and temperature not specified in the publication


organism: Schizosaccharomyces pombe

substrate_name: CTP

substrate: CTP


substrate_name: Unknown

proof_lines:
at pH 7.5 and 25°C
Ki above 0.05 mM, at pH 7.5 and 25°C

substrate: Unknown


organism: Entamoeba histolytica

proof_lines: pH 5.5, 37°C, recombinant mutant H140A cytoplasmic catalytic domain, no metal added

proof_lines: pH 5.5, 37°C, recombinant mutant H140A cytoplasmic catalytic domain, metal added


proof_lines: pH 5.5, 37°C, recombinant mutant H43A cytoplasmic catalytic domain, no metal added

proof_lines: pH 5.5, 37°C, recombinant mutant H43A cytoplasmic catalytic domain, metal added


proof_lines: pH 5.5, 37°C, recombinant wild-type cytoplasmic catalytic domain, no metal added

proof_lines: pH 5.5, 37°C, recombinant wild-type cytoplasmic catalytic domain, metal added


enzyme_display_name: 5'-deoxyadenosine deaminase

organism: Methanocaldococcus jannaschii

proof_lines: pH and temperature not specified in the publication, mutant enzyme Y136R/E150R

enzyme: 5'-deoxyadenosine deaminase


proof_lines: pH and temperature not specified in the publication, wild-type enzyme


enzyme_display_name: 22alpha-hydroxysteroid 23-monooxygenase

organism: Arabidopsis thaliana

substrate_name: NADPH; O2; 22alpha-Hydroxy-campesterol; H+

enzyme: 22alpha-hydroxysteroid 23-monooxygenase


substrate: NADPH; O2; 22alpha-Hydroxy-campesterol; H+


organism: Mycobacterium tuberculosis

substrate_name: UDP-glucose; Glucose 6-phosphate

substrate: UDP-glucose; Glucose 6-phosphate


substrate_name: D-Glucose 6-phosphate; UDPglucose

substrate: D-Glucose 6-phosphate; UDPglucose


organism: Sulfolobus acidocaldarius

substrate_name: UDP-alpha-D-glucose

substrate: UDP-alpha-D-glucose


organism: Thermoplasma acidophilum

substrate_name: D-glucose 6-phosphate

substrate: D-glucose 6-phosphate


mutation_signature: N-loop truncation mutant enzyme

proof_lines: pH 6.0, 60°C, N-loop truncation mutant enzyme


organism: Thermus thermophilus

substrate_name: UDP-glucose

proof_lines: recombinant enzyme, pH 7.0, 80°C, in presence of Mg2+

substrate: UDP-glucose


organism: Phaseolus lunatus

substrate_name: UDP-D-glucose; Zeatin

substrate: UDP-D-glucose; Zeatin


enzyme_display_name: succinylornithine transaminase

organism: Escherichia coli

substrate_name: L-ornithine

assay_conditions_summary: pH 8

proof_lines: pH 8.0, temperature not specified in the publication

enzyme: succinylornithine transaminase

substrate: L-ornithine


enzyme_display_name: mitogen-activated protein kinase kinase

organism: Homo sapiens

proof_lines:
pH 7.0, 25°C
Raw SKiD Env: Temp=25°C, pH=7

enzyme: mitogen-activated protein kinase kinase


proof_lines: kcat is 0.12 min(-1) for (22S)-22-hydroxycampesterol (PubMed:17138693). kcat is 1.27 min(-1) for (22S,24R)-22-hydroxy-5-alpha-ergostan-3-one (PubMed:17138693). kcat is 1.01 min(-1) for (22S,24R)-22-hydroxyergost-4-en-3-one (PubMed:17138693)


proof_lines: kcat is 0.11 min(-1) for (22S)-22-hydroxycampesterol (PubMed:17138693). kcat is 2.75 min(-1) for (22S,24R)-22-hydroxy-5-alpha-ergostan-3-one (PubMed:17138693). kcat is 1.40 min(-1) for (22S,24R)-22-hydroxyergost-4-en-3-one (PubMed:17138693)


organism: Escherichia coli (strain K12)

substrate_name: UDP-Glc

proof_lines: kcat is 34 sec(-1) for glucosyltransferase activity.

substrate: UDP-Glc


organism: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

substrate_name: 5'-deoxyadenosine (at pH 9 and 60 degrees Celsius)

assay_conditions_summary: pH 9

proof_lines: kcat is 120000 sec(-1) for 5'-deoxyadenosine deamination. kcat is 160 sec(-1) for S-methyl-5'-thioadenosine deamination. kcat is 1300 sec(-1) for S-adenosyl-L-homocysteine deamination. kcat is 110 sec(-1) for adenosine deamination (at pH 9


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)


organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843)


enzyme_display_name: GDP polyribonucleotidyltransferase

organism: Vesicular stomatitis Indiana virus (strain San Juan)

enzyme: GDP polyribonucleotidyltransferase


organism: Bos taurus

substrate_name: UDP-N-acetyl-alpha-D-glucosamine

substrate: UDP-N-acetyl-alpha-D-glucosamine


enzyme_display_name: phosphatidylglycerophosphatase

substrate_name: phosphatidylglycerophosphate

enzyme: phosphatidylglycerophosphatase

substrate: phosphatidylglycerophosphate


substrate_name: undecaprenyl pyrophosphate

substrate: undecaprenyl pyrophosphate


enzyme_display_name: [myosin-light-chain] phosphatase

organism: Oryctolagus cuniculus

substrate_name: phosphorylase

enzyme: [myosin-light-chain] phosphatase

substrate: phosphorylase


enzyme_display_name: fructan beta-(2,1)-fructosidase

organism: Triticum aestivum

enzyme: fructan beta-(2,1)-fructosidase


organism: Sphingobium xenophagum

substrate_name: 2,6-DHBP (at pH 7)

substrate: 2,6-DHBP (at pH 7)


assay_conditions_summary: cofactor CoA


substrate_name: glucose 6-phosphate

proof_lines: bifunctional fusion enzyme of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase

substrate: glucose 6-phosphate


organism: Halorhodospira halochloris

substrate_name: UDPglucose

substrate: UDPglucose


organism: Hyalophora cecropia


organism: Metarhizium anisopliae


organism: Pleurotus ostreatus


organism: Propionibacterium freudenreichii

assay_conditions_summary: pH 5.5; 310.15 K; cofactor ADP

proof_lines:
37°C, pH 5.5
37°C, pH 5.5, cosubstrate: TDP-glucose
37°C, pH 5.5, cosubstrate: GDP-glucose


organism: Burkholderia cepacia


organism: Pseudomonas aeruginosa


organism: Bacillus subtilis

substrate_name: phosphatidic acid

proof_lines:
pH 7.5, 25°C, with 0.45 mM CTP
pH 7.5, 25°C

substrate: phosphatidic acid


organism: Catharanthus roseus

substrate_name: CTP

substrate: CTP


organism: Cavia porcellus

substrate_name: phosphatidate

substrate: phosphatidate


organism: Clostridium perfringens


organism: Saccharomyces cerevisiae

substrate_name: D-Glucose 6-phosphate; UDPglucose

substrate: D-Glucose 6-phosphate; UDPglucose


organism: Escherichia coli


organism: Sus scrofa


substrate_name: dehydroepiandrosterone

proof_lines: inhibition of hydroxylation of dehydroepiandrosterone

substrate: dehydroepiandrosterone


organism: Homo sapiens


organism: Rattus norvegicus


enzyme_display_name: 1,8-cineole 2-exo-monooxygenase

proof_lines: pH 7.4, 37°C, formation of 2-hydroxy-1,8-cineole by isoform CYP3A4

proof_lines: pH 7.4, 37°C, formation of 3-hydroxy-1,8-cineole by isoform CYP3A4

enzyme: 1,8-cineole 2-exo-monooxygenase


proof_lines: pH 7.4, 37°C, after treatment of animals with pregenolone-16alpha-carbonitrile

proof_lines: pH 7.4, 37°C, animals treated with pregnenolone 16alpha-carbonitrile


enzyme_display_name: choloyl-CoA hydrolase

organism: Mus musculus

substrate_name: acetyl-CoA

proof_lines: In summary, KM for medium- to long-chain acyl CoAs is in the order 1.4-6.7 uM, and with short-chain acyl CoAs range from 8 to 30 uM. KM for bile acid-CoA esters is in the range 6-15 uM.

enzyme: choloyl-CoA hydrolase

substrate: acetyl-CoA


organism: Eubacterium sp.

substrate_name: cholyl-CoA

assay_conditions_summary: pH 7; 310.15 K; cofactor CoA

substrate: cholyl-CoA


substrate_name: dithiothreitol

assay_conditions_summary: pH 8; 310.15 K; cofactor CoA

proof_lines: pH 8.0, 37°C, with chenodeoxycholoyl-CoA

proof_lines: pH 8.0, 37°C, postnuclear fraction of liver homogenate

substrate: dithiothreitol


substrate_name: taurine toward choloyl-CoA

substrate: taurine toward choloyl-CoA


substrate_name: decanoyl-CoA

substrate: decanoyl-CoA


organism: Lithospermum erythrorhizon


organism: Pinus densiflora


organism: Punica granatum

substrate_name: gallic acid (at pH 5.0 and 30 degrees Celsius)

assay_conditions_summary: pH 5

proof_lines: kcat is 0.55 sec(-1) with gallic acid as substrate. kcat is 0.6 sec(-1) with 4-hydroxybenzoic acid as substrate. kcat is 1.35 sec(-1) with 3,4-dihydroxybenzoic acid as substrate. kcat is 0.89 sec(-1) with caffeic acid as substrate. kcat is


proof_lines: kcat is 0.52 sec(-1) with gallic acid as substrate. kcat is 0.61 sec(-1) with 4-hydroxybenzoic acid as substrate. kcat is 0.76 sec(-1) with 3,4-dihydroxybenzoic acid as substrate. kcat is 0.64 sec(-1) with caffeic acid as substrate. kcat is

substrate: gallic acid (at pH 5.0 and 30 degrees Celsius)


organism: Paenibacillus borealis

proof_lines: kcat is 102 sec(-1) with O-beta-D-glucosyl-D-glucuronate as substrate (PubMed:35947916). kcat is 156 sec(-1) with alpha-D-glucose 1-phosphate as substrate (PubMed:35947916). kcat is 351 sec(-1) with D-glucuronic acid as substrate (PubMed:35


enzyme_display_name: guanosine deaminase

organism: Arabidopsis thaliana

enzyme: guanosine deaminase


organism: Camellia sinensis

substrate_name: guanosine

substrate: guanosine


organism: Pseudomonas putida

substrate_name: deoxyguanosine

substrate: deoxyguanosine


enzyme_display_name: leukotriene-C4 hydrolase

substrate_name: glutathione

enzyme: leukotriene-C4 hydrolase

substrate: glutathione


substrate_name: glycylglycine

substrate: glycylglycine


substrate_name: leukotriene C(4)

substrate: leukotriene C(4)


substrate_name: reduced glutathione

proof_lines: in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37°C

substrate: reduced glutathione


substrate_name: S-decyl-GSH

assay_conditions_summary: pH 8; 310.15 K; buffer 0.1 M Tris-HCl

proof_lines: in 0.1 M Tris-HCl buffer, pH 8.0, at 37°C

proof_lines: pH and temperature not specified in the publication

substrate: S-decyl-GSH


enzyme_display_name: creatinine deaminase

organism: Clostridium paraputrificum

substrate_name: creatinine

enzyme: creatinine deaminase

substrate: creatinine


organism: Corynebacterium glutamicum

proof_lines:
pH 7.5, 37°C
soluble enzyme
immobilized enzyme


organism: Cryptococcus neoformans


organism: Flavobacterium filamentosum

substrate_name: cytosine

substrate: cytosine


organism: uncultured bacterium


organism: Unknown

substrate_name: UDPglucose

substrate: UDPglucose


enzyme_display_name: NAD-dependent lipoamidase

substrate_name: [histone H3]-N6-acetyl-L-lysine

enzyme: NAD-dependent lipoamidase

substrate: [histone H3]-N6-acetyl-L-lysine


enzyme_display_name: caffeine synthase

organism: Coffea arabica

enzyme: caffeine synthase


substrate_name: S-Adenosyl-L-methionine; Theobromine

substrate: S-Adenosyl-L-methionine; Theobromine


enzyme_display_name: [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M

organism: Methanosarcina barkeri

proof_lines:
isozyme MT2-M, pH 7.2, 23°C
isozyme MT2-A, pH 7.2, 23°C
isozymes MT2-A and MT-2-M, pH 7.2, 23°C


organism: Ricinus communis

substrate_name: [histone H3.1]-L-lysine27

substrate: [histone H3.1]-L-lysine27


mutation_signature: PHD domain deletion mutant

mutation_type: deletion

proof_lines: PHD domain deletion mutant, pH 8.0, 30°C


enzyme_display_name: cocaine synthase

organism: Erythroxylum coca

substrate_name: ecgonine methyl ester

assay_conditions_summary: pH 9.4; 293.15 K; cofactor CoA

proof_lines: with benzoyl-CoA as sosubstrate, at pH 9.4 and 20°C

proof_lines: with cinnamoyl-CoA as sosubstrate, at pH 9.4 and 20°C

enzyme: cocaine synthase

substrate: ecgonine methyl ester


organism: Mesocricetus auratus


organism: Rattus norvegicus


enzyme_display_name: phosphoribosylanthranilate isomerase

organism: Streptomyces coelicolor

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

proof_lines:
PriA, PRA isomerase activity
Raw SKiD Env: Temp=-----, pH=-----

source_databases_merged: sabio_rk brenda skid

enzyme: phosphoribosylanthranilate isomerase


proof_lines: PriA mutant R19A, PRA isomerase activity


organism: Thermotoga maritima


enzyme_display_name: 5-formyltetrahydrofolate cyclo-ligase

organism: Arabidopsis thaliana

mutation_signature: truncated recombinant enzyme

proof_lines: truncated recombinant enzyme, pH 6.0, 23°C

enzyme: 5-formyltetrahydrofolate cyclo-ligase


organism: Salmonella enterica subsp. enterica serovar Typhimurium


organism: Paenibacillus borealis

substrate_name: 4-nitrophenyl beta-D-glucuronide

substrate: 4-nitrophenyl beta-D-glucuronide


enzyme_display_name: guanidinoacetate kinase

organism: Namalycastis sp.

substrate_name: glycocyamine

proof_lines:
recombinant GK beta
native GK

enzyme: guanidinoacetate kinase

substrate: glycocyamine


enzyme_display_name: alpha-mannan endo-1,2-alpha-mannanase

organism: Bacteroides thetaiotaomicron

enzyme: alpha-mannan endo-1,2-alpha-mannanase


organism: Bacteroides xylanisolvens


enzyme_display_name: 5'-deoxyadenosine deaminase

organism: Methanocaldococcus jannaschii

proof_lines: pH and temperature not specified in the publication, mutant enzyme Y136R

enzyme: 5'-deoxyadenosine deaminase


proof_lines: pH and temperature not specified in the publication, mutant enzyme E150R


enzyme_display_name: GTP 3',8-cyclase

organism: Staphylococcus aureus

substrate_name: GTP

enzyme: GTP 3',8-cyclase

substrate: GTP


substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


enzyme_display_name: D-glutamate cyclase

organism: Mus musculus

substrate_name: D-Glutamate

enzyme: D-glutamate cyclase

substrate: D-Glutamate


substrate_name: D-glutamate

proof_lines:
colorimetric assay, pH 8.0, 37°C
pH 8.0, 37°C

substrate: D-glutamate


enzyme_display_name: Transferred entry: 3.5.99.14

organism: Thalictrum flavum

source_db: skid

source_license: CC BY-NC-ND 4.0

enzyme: Transferred entry: 3.5.99.14

source: skid


organism: Thalictrum flavum subsp. glaucum


organism: Cucurbita pepo


organism: Malus domestica

substrate_name: S-Adenosyl-L-methionine

substrate: S-Adenosyl-L-methionine


organism: Vigna radiata


enzyme_display_name: adenylate cyclase

organism: Yersinia pestis

substrate_name: ATP

assay_conditions_summary: pH 8.5; 310.15 K; cofactor ATP

proof_lines: mutant enzyme L72A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme L72A, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C

enzyme: adenylate cyclase

substrate: ATP


proof_lines: mutant enzyme R113A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C


proof_lines: mutant enzyme M140A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme M140A, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C


proof_lines: mutant enzyme E12Q, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme E12Q, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C


proof_lines: mutant enzyme K14A, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme K14A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C


organism: Escherichia coli


organism: Geobacillus thermopakistaniensis


organism: Saccharomyces cerevisiae


organism: Silene dioica

substrate_name: UDP-glucose

proof_lines: reaction with cyanidin 3-rhamnosylglucoside at pH 7.4

substrate: UDP-glucose


organism: Nephtys caeca

substrate_name: ADP

assay_conditions_summary: cofactor ATP

substrate: ADP


organism: Perinereis sp.

assay_conditions_summary: pH 8.1; 298.15 K; cofactor ATP


enzyme_display_name: T2-induced deoxynucleotide kinase

substrate_name: dGMP

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

proof_lines:
pH 7.4, 37°C
pH 7.4, 37°C, with dTMP
pH 7.4, 37°C, with 5-hydroxymethyl-dCMP

enzyme: T2-induced deoxynucleotide kinase

substrate: dGMP


organism: Microbacterium sediminis

substrate_name: D-glucose 6-phosphate

substrate: D-glucose 6-phosphate


organism: Mycobacterium tuberculosis

proof_lines:
with UDPglucose as glucosyl donor
with GDPglucose as glucosyl donor


organism: Mycolicibacterium smegmatis

substrate_name: UDPglucose

proof_lines: in presence of optimal concentration of a polyanion

substrate: UDPglucose


organism: Neurospora crassa


organism: Homo sapiens

substrate_name: complement component C5

proof_lines: high affinity C5 convertase (EAC1,C3bC4b,C2a)

proof_lines: surface-bound C5 convertase (EAC1,C4b,C2a)

proof_lines: 37°C, soluble monomeric C3/C5 convertase C4b,C2a

substrate: complement component C5


organism: Mus musculus


enzyme_display_name: 4-acetamidobutyryl-CoA deacetylase

organism: Pseudomonas sp.

proof_lines: estimated from rates at lower and higher substrate concentration

enzyme: 4-acetamidobutyryl-CoA deacetylase


organism: Brevundimonas diminuta

substrate_name: N-myristoyl-L-glutamate

substrate: N-myristoyl-L-glutamate


organism: Penicillium sp.

substrate_name: N-oleoyl-L-glutamate

substrate: N-oleoyl-L-glutamate


enzyme_display_name: diguanidinobutanase

organism: Pseudomonas putida

enzyme: diguanidinobutanase


enzyme_display_name: gentisate 1,2-dioxygenase

organism: Corynebacterium glutamicum

enzyme: gentisate 1,2-dioxygenase


organism: Escherichia coli


organism: Pseudaminobacter salicylatoxidans

proof_lines: pH 8.0, temperature not specified in the publication


organism: Pseudomonas alcaligenes

substrate_name: O2; Gentisate

substrate: O2; Gentisate


organism: Rhodococcus opacus


organism: Ruegeria pomeroyi


organism: Xanthobacter polyaromaticivorans

substrate_name: 1-hydroxy 2 naphthoate

substrate: 1-hydroxy 2 naphthoate


organism: Aquipseudomonas alcaligenes

assay_conditions_summary: pH 7.4; 296.15 K; buffer 0.1 M phosphate

proof_lines: in 0.1 M phosphate buffer, pH 7.4, at 23°C


organism: Comamonas testosteroni

substrate_name: gentisate

substrate: gentisate


organism: Delftia acidovorans


organism: Faucicola osloensis


proof_lines: mutant enzyme Y181F, in 0.1 M KH2PO4 buffer (pH 7.4) at 23°C


proof_lines: wild type enzyme, in 0.1 M KH2PO4 buffer (pH 7.4) at 23°C


enzyme_display_name: UDP-glucuronate 4-epimerase

organism: Arabidopsis thaliana

substrate_name: UDPglucuronate

enzyme: UDP-glucuronate 4-epimerase

substrate: UDPglucuronate


substrate_name: UDP-glucuronate

proof_lines: recombinant AtUGlcAE3, lacking the amino acid position 1-53, in 0.1 M sodium phosphate, pH 7.6

proof_lines: recombinant AtUGlcAE3, position 1-53 is lacking

substrate: UDP-glucuronate


proof_lines: recombinant AtUGlcAE2, lacking the amino acid position 1-68, in 0.1 M sodium phosphate, pH 7.6

proof_lines: recombinant AtUGlcAE2, position 1-68 is lacking


organism: Oryza sativa

proof_lines: recombinant OsUGlcAE3, lacking the amino acid position 1-53, in 0.1 M sodium phosphate, pH 7.6

proof_lines: recombinant OsUGlcAE3, position 1-53 is lacking


organism: Zea mays

proof_lines: recombinant ZmUGlcAE, lacking the putative transmembrane domain at amino acid position 1-47, in 0.1 M sodium phosphate, pH 7.6

proof_lines: recombinant ZmUGlcAE, position 1-47 is lacking


enzyme_display_name: alpha-methylacyl-CoA racemase

organism: Homo sapiens

substrate_name: (S)-Pristanoyl-CoA

assay_conditions_summary: pH 7.2; 303.15 K; cofactor CoA

proof_lines: recombinant enzyme, 50 mM NaH2PO4-NaOH, pH 7.2

proof_lines: pH 7.4, 30°C, recombinant human AMACR 1A

proof_lines: pH not specified in the publication, 37°C

enzyme: alpha-methylacyl-CoA racemase

substrate: (S)-Pristanoyl-CoA


organism: Rattus norvegicus


substrate_name: salicylate

substrate: salicylate


proof_lines: wild-type enzyme, pH and temperature not specified in the publication


organism: Pseudomonas putida

assay_conditions_summary: pH 7.4; 296.15 K; buffer assay context


organism: Ralstonia solanacearum


organism: Anabaena flos-aquae


organism: Bacillus cereus

substrate_name: UDP-D-galacturonate

substrate: UDP-D-galacturonate


organism: Dolichospermum flos-aquae


organism: Streptococcus pneumoniae

substrate_name: UDP-D-glucuronate

substrate: UDP-D-glucuronate


proof_lines: pH and temperature not specified in the publication

proof_lines: anti-enantiomer, pH not specified in the publication, temperature not specified in the publication


organism: Mycobacterium tuberculosis

substrate_name: (R)-pristanoyl-CoA

substrate: (R)-pristanoyl-CoA


proof_lines: recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C

proof_lines: recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C


substrate_name: trihydroxycoprostanoyl-CoA

substrate: trihydroxycoprostanoyl-CoA


enzyme_display_name: D-ribulokinase

substrate_name: D-ribulose

enzyme: D-ribulokinase

substrate: D-ribulose


organism: Saccharomyces cerevisiae


proof_lines: recombinant AtUGlcAE1, position 1-64 is lacking


organism: Klebsiella aerogenes

substrate_name: D-arabitol

assay_conditions_summary: pH 7.8; 301.15 K; cofactor ATP

substrate: D-arabitol


enzyme_display_name: fatty-acid peroxygenase

organism: Bacillus subtilis

substrate_name: cumene hydroperoxide

assay_conditions_summary: pH 6.7; 298.15 K; buffer 0.1 M potassium phosphate

proof_lines: mutant enzyme R242A, using myristic acid as cosubstrate, in 0.1 M potassium phosphate buffer (pH 6.7), at 25°C

enzyme: fatty-acid peroxygenase

substrate: cumene hydroperoxide


proof_lines: mutant enzyme R242K, using myristic acid as cosubstrate, in 0.1 M potassium phosphate buffer (pH 6.7), at 25°C


proof_lines: wild type enzyme, using myristic acid as cosubstrate, in 0.1 M potassium phosphate buffer (pH 6.7), at 25°C


organism: Sphingomonas paucimobilis

substrate_name: myristic acid

substrate: myristic acid


organism: Hyphomicrobium sulfonivorans

substrate_name: Dimethyl sulfide

substrate: Dimethyl sulfide


organism: Arthrobacter globiformis

substrate_name: FMN

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADH

substrate: FMN


enzyme_display_name: sphingolipid 4-desaturase

substrate_name: Unknown

enzyme: sphingolipid 4-desaturase

substrate: Unknown


substrate_name: NADH

assay_conditions_summary: pH 7.4; 310.15 K; cofactor NADH

proof_lines: with N-octanoyl-D-erythro-C18-sphinganine as cosubstrate, at pH 7.4 and 37°C

substrate: NADH


enzyme_display_name: formate dehydrogenase (acceptor)

organism: Escherichia coli

substrate_name: formate

enzyme: formate dehydrogenase (acceptor)

substrate: formate


proof_lines: pH 7.5, 24°C, mutant form of the enzyme in which cysteine replaces selenocysteine


organism: Prototheca zopfii

substrate_name: Malonate semialdehyde; CoA; NAD+

assay_conditions_summary: cofactor NAD+

substrate: Malonate semialdehyde; CoA; NAD+


substrate_name: Coenzyme A; NAD+; 3-Oxopropanoate

substrate: Coenzyme A; NAD+; 3-Oxopropanoate


organism: Pseudomonas sp.


organism: Rattus norvegicus

substrate_name: CoA

substrate: CoA


enzyme_display_name: glutamate racemase

organism: Bacillus anthracis

proof_lines: potent competitive inhibitor for RacE2 mutant V149A

enzyme: glutamate racemase


substrate_name: D-glutamate

assay_conditions_summary: pH 9.2; 298.15 K; cofactor NAD+

proof_lines: recombinant wild type enzyme, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml d

proof_lines: recombinant wild type enzyme, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol

proof_lines: recombinant wild type enzyme, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/mL d

substrate: D-glutamate


proof_lines: RacE2 mutant enzyme S207A, pH and temperature not specified in the publication


substrate_name: L-glutamate

substrate: L-glutamate


organism: Streptomyces davaonensis

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


enzyme_display_name: salutaridinol 7-O-acetyltransferase

organism: Papaver somniferum

substrate_name: nudaurine

assay_conditions_summary: cofactor CoA

proof_lines:
with salutaridinol as substrate
with nudaurine as substrate

enzyme: salutaridinol 7-O-acetyltransferase

substrate: nudaurine


enzyme_display_name: plant seed peroxygenase

organism: Avena sativa

enzyme: plant seed peroxygenase


organism: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)


enzyme_display_name: type II protein arginine methyltransferase

organism: Caenorhabditis elegans

substrate_name: histone H4 (at 37 degrees Celsius)

substrate: histone H4 (at 37 degrees Celsius)


substrate_name: salutaridinol

substrate: salutaridinol


enzyme_display_name: diaminobutyrate acetyltransferase

organism: Methylotuvimicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z)

enzyme: diaminobutyrate acetyltransferase


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: UDP-glucose

substrate: UDP-glucose


substrate_name: UDP-Glc (in the presence of polyanion)

substrate: UDP-Glc (in the presence of polyanion)


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: D-glucose 6-phosphate

substrate: D-glucose 6-phosphate


organism: Streptomyces davaonensis (strain DSM 101723 / JCM 4913 / KCC S-0913 / 768)

substrate_name: AF

proof_lines: kcat is 22.0 min(-1) with AF as substrate. kcat is 14.8 min(-1) with S-adenosyl-L-methionine as substrate.

substrate: AF


enzyme_display_name: 4-hydroxybenzoate polyprenyltransferase

substrate_name: farnesyl diphosphate

enzyme: 4-hydroxybenzoate polyprenyltransferase

substrate: farnesyl diphosphate


organism: Oryza sativa

substrate_name: geranyl diphosphate

substrate: geranyl diphosphate


organism: Pseudomonas putida

substrate_name: nonaprenyl diphosphate

substrate: nonaprenyl diphosphate


enzyme_display_name: galactan 1,3-beta-galactosidase

organism: Irpex lacteus

enzyme: galactan 1,3-beta-galactosidase


enzyme_display_name: ([Pyruvate, phosphate dikinase] phosphate) phosphotransferase

organism: Zea mays

substrate_name: phosphate

proof_lines: phosphorylated at a Thr residue, pH 8.3, temperature not specified in the publication

substrate: phosphate


organism: Bifidobacterium longum

substrate_name: H2O; Arabinogalactan

substrate: H2O; Arabinogalactan


organism: Raphanus sativus


enzyme_display_name: gingipain K

organism: Porphyromonas gingivalis

substrate_name: hemoglobin

proof_lines:
pH 7.6, 37°C, human serum hemoglobin
pH 8.5, 37°C

enzyme: gingipain K

substrate: hemoglobin


organism: Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)

substrate_name: pABA (at 70 degrees Celsius)

substrate: pABA (at 70 degrees Celsius)


organism: Escherichia coli (strain K12)

substrate_name: GPP

substrate: GPP


organism: Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)


enzyme_display_name: succinylornithine transaminase

organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

substrate_name: N(2)-succinyl-L-ornithine (at 15 mM 2-ketoglutarate and pH 9.0)

assay_conditions_summary: pH 9

enzyme: succinylornithine transaminase


organism: Homo sapiens

substrate_name: D-ribulose

substrate: D-ribulose


organism: Arabidopsis thaliana

substrate_name: ADP (at pH 6.8)

assay_conditions_summary: pH 6.8; cofactor ADP

enzyme: ([Pyruvate, phosphate dikinase] phosphate) phosphotransferase

substrate: ADP (at pH 6.8)


substrate_name: ADP

assay_conditions_summary: cofactor ADP

substrate: ADP


enzyme_display_name: phosphatidate cytidylyltransferase

enzyme: phosphatidate cytidylyltransferase


organism: Shigella sp. CD2

substrate_name: myo-inositol hexakisphosphate

substrate: myo-inositol hexakisphosphate


proof_lines: pH 5.5, 37°C, recombinant glycosylated enzyme expressed from Pichia pastoris

proof_lines: pH 5.5, 37°C, recombinant non-glycosylated enzyme expressed from Escherichia coli


enzyme_display_name: adenylate cyclase

organism: Bacillus anthracis

substrate_name: ATP

assay_conditions_summary: pH 8; 298.15 K; cofactor ATP

proof_lines:
mutant H351F, pH 8.0, 25°C
mutant H351F

enzyme: adenylate cyclase

substrate: ATP


proof_lines:
wild type, pH 8.0, 25°C
wild type


proof_lines:
mutant H351N, pH 8.0, 25°C
mutant H351N


enzyme_display_name: glucan endo-1,3-alpha-glucosidase

organism: Niallia circulans

proof_lines: recombinant wild-type enzyme, pH 6.5, 30°C

enzyme: glucan endo-1,3-alpha-glucosidase


proof_lines: recombinant mutant AglDELTADS1, pH 6.5, 30°C

proof_lines: recombinant mutant AglDELTADS1CB6DS2, pH 6.5, 30°C

proof_lines: recombinant mutant AglDELTADS1CB6, pH 6.5, 30°C


organism: Lilium longiflorum

proof_lines:
in presence of Ca2+
37°C, pH 8.0
37°C, pH 7.0


organism: Sporolactobacillus laevolacticus

substrate_name: Na-phytate

substrate: Na-phytate


organism: Pseudomonas sp.

substrate_name: limit glucan

substrate: limit glucan


organism: Streptomyces chartreusis

substrate_name: Glucan

substrate: Glucan


organism: Trichoderma viride

substrate_name: pseudonigeran

substrate: pseudonigeran


organism: Pseudomonas aeruginosa

substrate_name: isochorismate

proof_lines: i.e. 2-amino-2-deoxy-isochorismate, pH 6.5

substrate: isochorismate


proof_lines: kcat is 7.3 sec(-1) with 2-amino-2-deoxyisochorismate as substrate. kcat is 1.2 sec(-1) with chorismate as substrate. kcat is 0.2 sec(-1) with isochorismate as substrate. kcat is 0.02 sec(-1) with 4-amino-4-deoxychorismate as substrate.


organism: Thermotoga maritima


proof_lines:
25°C, pH 7.5, wild-type enzyme
37°C, pH 7.5, wild-type enzyme
80°C, pH 7.5, wild-type enzyme


organism: Escherichia coli


organism: Streptomyces coelicolor


enzyme_display_name: V-cath endopeptidase

substrate_name: Z-Phe-Arg 7-amido-4-methylcoumarin

enzyme: V-cath endopeptidase

substrate: Z-Phe-Arg 7-amido-4-methylcoumarin


organism: Helicobacter pylori


substrate_name: Polyphosphate

substrate: Polyphosphate


enzyme_display_name: formylmethionine deformylase

organism: Rattus norvegicus

substrate_name: H2O; N-Formyl-L-methionine

proof_lines:
enzyme from liver
enzyme from spleen
enzyme from brain, heart or muscle

enzyme: formylmethionine deformylase

substrate: H2O; N-Formyl-L-methionine


organism: Euglena gracilis

substrate_name: N-formyl-methionine

substrate: N-formyl-methionine