organism: Brassica napus

proof_lines: pH 8.4, 24°C, pH 7.3, 24°C, enzyme from cells grown in presence of phosphate or in absence of phosphate, assay in presence of glycerol

proof_lines: pH 7.3, 24°C, enzyme from cells grown in absence of phosphate, assay in absence of glycerol

proof_lines: pH 7.3, 24°C, enzyme from cells grown in presence of phosphate, assay in absence of glycerol


enzyme_display_name: phosphoenolpyruvate carboxylase

organism: Brassica rapa subsp. oleifera

substrate_name: phosphoenolpyruvate

source_db: brenda

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

enzyme: phosphoenolpyruvate carboxylase

substrate: phosphoenolpyruvate

source: brenda


organism: Acetobacter aceti


organism: Amaranthus hypochondriacus

proof_lines: pH 7.3, addition of 30 mM phosphate, enzyme from dark-adapted leaves

proof_lines: pH 7.3, no addition of phosphate, enzyme from dark-adapted leaves


organism: Amaranthus viridis


organism: Ancylobacter novellus


organism: Cucumis sativus

proof_lines: pH 8.0, 25°C, enzyme from roots grown in absence of Fe2+

proof_lines: pH 8.0, 25°C, enzyme from roots grown in presence of Fe2+


organism: Escherichia coli


organism: Fagus sylvatica

proof_lines: stem leaf


organism: Haloxylon persicum

proof_lines: leaves are collected after 5 h into the dark, pH: 8.0

proof_lines: leaves are collected after 5 h into the light, pH: 8.0


organism: Hydrilla verticillata

proof_lines: C3 extract, dark-harvested PEPC in the absence of D-glucose 6-phosphate

proof_lines: C3 extract, light-harvested PEPC in the absence of D-glucose 6-phosphate

proof_lines: C3 extract, light-harvested PEPC in the presence of 5 mM D-glucose 6-phosphate


organism: Kalanchoe fedtschenkoi

proof_lines:
at 40°C
in absence of effectors, at 25°C
at 14°C


organism: Lupinus luteus

proof_lines:
enzyme form PEPC II
enzyme form PEPC III
enzyme form PEPC I


organism: Malus sp.

proof_lines: golden delicious, vascular bundle or seed


organism: Methanothermobacter thermautotrophicus


organism: Methanothermus sociabilis


organism: Molinema dessetae


organism: Oceanimonas smirnovii

proof_lines: 23°C, pH not specified in the publication


organism: Oryza sativa

proof_lines:
isoform Osppc2a, at pH 7.3 and 30°C
isoform Osppc2a, at pH 8.0 and 30°C
isoform Osppc4, at pH 8.0 and 30°C


organism: Paspalum vaginatum

proof_lines:
wild-type, pH 7.6, 25°C
wild-type, Hill value 1.1, pH 7.6, 25°C


organism: Phaeodactylum tricornutum


organism: Picosynechococcus sp. PCC 7002


organism: Plasmodium berghei


organism: Rhodopseudomonas sp.

assay_conditions_summary: cofactor CoA


organism: Ricinus communis

assay_conditions_summary: pH 8

proof_lines:
pH 8, PEPC1
pH 8, PEPC2
pH 7.3, PEPC1


organism: Sorghum bicolor

proof_lines:
10 days post-imbibition, pH 7.3, 30°C
extract from germinated seed
35 days post-anthesis, pH 7.3, 30°C


organism: Spinacia oleracea

proof_lines:
with Mg2+ as activator
with Mn2+ as activator
without activator


organism: Steinchisma hians


organism: Suaeda aralocaspica


organism: Suaeda linifolia


organism: Thermosynechococcus vestitus


organism: Thiobacillus thioparus


organism: Vicia faba

assay_conditions_summary: pH 7

proof_lines:
pH 7
pH 8.3


organism: Zea mays subsp. mays


enzyme_display_name: phosphoenolpyruvate carboxykinase (GTP)

organism: Aquarana catesbeiana

substrate_name: ITP

proof_lines:
exchange reaction
pH 6.3
CO2 in form of HCO3-

enzyme: phosphoenolpyruvate carboxykinase (GTP)

substrate: ITP


organism: Ascaris suum

substrate_name: GDP

proof_lines: with GDP as cosubstrate, Mn2+ does not affect the Km

proof_lines: with IDP as cosubstrate, Mn2+ does not affect the Km

substrate: GDP


organism: Corynebacterium glutamicum

proof_lines:
pH 6.8
CO2 in form of HCO3-


organism: Euglena gracilis

proof_lines: enzyme from heterotrophically grown cells


organism: Gallus gallus

proof_lines: enzyme form M1 from embryo and enzyme form S2 from young chicken


organism: Homo sapiens

proof_lines: in 100 mM HEPES-NaOH, pH 8, 3.6 mM L-malate, 10 mM dithiothreitol, 2 mM MgCl2, 0.2 mM MnCl2, at 37°C

proof_lines: in 100 mM HEPES-NaOH, pH 7.2, 100 mM KHCO3, 10 mM dithiothreitol, 2 mM MgCl2, 0.2 mM MnCl2, at 37°C


organism: Modiolus demissus

substrate_name: GTP

proof_lines: in presence of 1 mM MnCl2 and 1 mM MgCl2

substrate: GTP


organism: Perumytilus purpuratus

substrate_name: IDP

proof_lines:
pH 7, in presence of 2.3 mM Mn2+
CO2 in form of HCO3-

substrate: IDP


organism: Raillietina echinobothrida

proof_lines: with GDP as cosubstrate, in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication

proof_lines: with IDP as cosubstrate, in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication


organism: Rattus norvegicus


organism: Squalus acanthias

proof_lines:
in presence of 0.0015 mM Mn2+
in presence of 0.0013 mM Mn2+
in presence of 0.002 mM Mn2+


enzyme_display_name: 2-dehydro-3-deoxy-L-fuconate aldolase

organism: Campylobacter jejuni subsp. jejuni

enzyme: 2-dehydro-3-deoxy-L-fuconate aldolase


enzyme_display_name: (S)-hydroxynitrile lyase

organism: Hevea brasiliensis

substrate_name: (S)-mandelonitrile

assay_conditions_summary: pH 5; 298.15 K; buffer pH 6

proof_lines: in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication

proof_lines: in 50 mM phosphate buffer pH 6.0, at 25°C

proof_lines: in 50 mM phosphate buffer pH 6.0, at 22°C

enzyme: (S)-hydroxynitrile lyase

substrate: (S)-mandelonitrile


organism: Manihot esculenta

substrate_name: mandelonitrile

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution

substrate: mandelonitrile


assay_conditions_summary: pH 5; 313.15 K; buffer 50 mM citrate


enzyme_display_name: tryptophan synthase

organism: Thermotoga maritima

substrate_name: L-serine

proof_lines: pH 7.5, 80°C, recombinant (TrpA-TrpB1)2 tetrameric complex, beta-reaction

proof_lines: pH 7.5, 80°C, recombinant TrpB1, beta-reaction

enzyme: tryptophan synthase

substrate: L-serine


organism: Saccharomyces cerevisiae

substrate_name: indole

substrate: indole


organism: Salmonella enterica subsp. enterica serovar Typhimurium

km_display: Not reported

proof_lines: beta-reaction and overall-reaction, mutant D305A enzyme, pH 7.8, 25°C

proof_lines: beta-reaction, mutant D305A, pH 7.8, 25°C

proof_lines: overall-reaction, mutant D305A enzyme, pH 7.8, 25°C, in presence of K+


substrate_name: 1-(indol-3-yl)glycerol 3-phosphate

proof_lines: overall-reaction, mutant R141A, pH 7.8, 25°C

proof_lines: beta-reaction, mutant R141A, pH 7.8, 25°C

proof_lines: alpha-reaction, mutant R141A, pH 7.8, 25°C

substrate: 1-(indol-3-yl)glycerol 3-phosphate


proof_lines: 37°C, beta-elimination, enzyme in solution

proof_lines: in the presence of 100 mM CsCl, at pH 6.5 and 25°C


enzyme_display_name: alanine racemase

substrate_name: D-alanine

enzyme: alanine racemase

substrate: D-alanine


organism: Enterococcus faecalis

substrate_name: D-Ala

proof_lines: pH and temperature not specified in the publication

substrate: D-Ala


organism: Escherichia coli

proof_lines: in 50 mM potassium phosphate buffer pH 7.4, at 30°C


organism: Geobacillus stearothermophilus

substrate_name: L-Ala

substrate: L-Ala


organism: Procambarus clarkii


organism: Pseudomonas aeruginosa


organism: Pseudomonas fluorescens


organism: Pseudomonas putida

substrate_name: L-alanine

assay_conditions_summary: pH 9; 310.15 K; buffer pH 7

substrate: L-alanine


organism: Medicago sativa

proof_lines:
Vmax = 0.44 mol/s/kg
Vmax = 1.02 mol/s/kg


organism: Mycobacterium avium


organism: Mycobacterium tuberculosis

substrate_name: Unknown

ki_unit: mM

proof_lines: in 100 mM Tris-Tricine, pH 8.5, temperature not specified in the publication

substrate: Unknown


organism: Penaeus monodon


enzyme_display_name: aralkylamine N-acetyltransferase

substrate_name: histamine

source_db: oed

protein_accession_database: UniProt

assay_conditions_summary: pH 7; 298.15 K; cofactor CoA

enzyme: aralkylamine N-acetyltransferase

substrate: histamine

source: oed


substrate_name: acetyl-CoA

substrate: acetyl-CoA


substrate_name: octopamine

substrate: octopamine


substrate_name: propionyl-CoA

substrate: propionyl-CoA


organism: Mycolicibacterium smegmatis


organism: Oryza sativa

substrate_name: Acetyl-CoA; Serotonin

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

substrate: Acetyl-CoA; Serotonin


source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

assay_conditions_summary: pH 8.8; 318.15 K; cofactor CoA


organism: Ovis aries

substrate_name: Coenzyme A

assay_conditions_summary: pH 6.8; 303.15 K; cofactor CoA

substrate: Coenzyme A


substrate_name: N-Bromoacetyltryptamine

substrate: N-Bromoacetyltryptamine


enzyme_display_name: carnitine O-palmitoyltransferase

organism: Homo sapiens

substrate_name: Palmitoyl-CoA; L-Carnitine

enzyme: carnitine O-palmitoyltransferase

substrate: Palmitoyl-CoA; L-Carnitine


proof_lines: chimaera in which the distinctive N-terminal segment of ovine m-BPT 1 is replaced with that from rat M-CPT 1


organism: Rattus norvegicus


organism: Sus scrofa


substrate_name: Acetyl-CoA

substrate: Acetyl-CoA


substrate_name: Tryptamine

substrate: Tryptamine


substrate_name: alpha-Methyltryptamine

substrate: alpha-Methyltryptamine


substrate_name: serotonin

substrate: serotonin


substrate_name: Acetyl-CoA; Tryptamine

substrate: Acetyl-CoA; Tryptamine


enzyme_display_name: corrinoid adenosyltransferase

substrate_name: ATP

source_db: skid

source_license: CC BY-NC-ND 4.0

assay_conditions_summary: cofactor ATP

enzyme: corrinoid adenosyltransferase

substrate: ATP

source: skid


organism: Lactobacillus reuteri

substrate_name: Cob(I)alamin

substrate: Cob(I)alamin


assay_conditions_summary: pH 8; 310.15 K; cofactor ATP


substrate_name: CTP

substrate: CTP


substrate_name: dATP

substrate: dATP


substrate_name: UTP

substrate: UTP


substrate_name: ITP

substrate: ITP


source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

source_databases_merged: sabio_rk brenda skid


substrate_name: ATP; Cob(I)alamin

substrate: ATP; Cob(I)alamin


substrate_name: GTP

substrate: GTP


substrate_name: Cob(II)alamin

substrate: Cob(II)alamin


substrate_name: Cob(II)alamin; ATP

substrate: Cob(II)alamin; ATP


enzyme_display_name: kynurenine--oxoglutarate transaminase

smiles: CC(C)CC(=O)C(=O)O

enzyme: kynurenine--oxoglutarate transaminase


substrate_name: oxaloacetate

smiles: O=C(O)CC(=O)C(=O)O

substrate: oxaloacetate


substrate_name: Aminobutyrate

substrate: Aminobutyrate


substrate_name: L-Glutamate

substrate: L-Glutamate


substrate_name: L-Tryptophan

substrate: L-Tryptophan


substrate_name: L-Phenylalanine

substrate: L-Phenylalanine


substrate_name: L-Tyrosine

substrate: L-Tyrosine


substrate_name: Glyoxylate; L-Kynurenine

substrate: Glyoxylate; L-Kynurenine


substrate_name: L-kynurenine

source_license: CC BY 4.0; CC BY-NC-ND 4.0

source_databases_merged: brenda skid

substrate: L-kynurenine


organism: Mus musculus

substrate_name: L-tryptophan

substrate: L-tryptophan


smiles: O=C(O)CCC(=O)C(=O)O


substrate_name: ATP

substrate: ATP


substrate_name: Cob(I)alamin

substrate: Cob(I)alamin


organism: Salmonella enterica

substrate_name: cob(II)inamide

substrate: cob(II)inamide


organism: Thermoplasma acidophilum

substrate_name: dATP

assay_conditions_summary: pH 8; 343.15 K; cofactor ATP

proof_lines: pH 8.0, 70°C, wild-type enzyme, in presence of Mg2+

substrate: dATP


substrate_name: Cobalamin

proof_lines:
Raw SKiD Env: Temp=70°C, pH=8
Raw SKiD Env: Temp=-----, pH=-----

substrate: Cobalamin


organism: Aedes aegypti

substrate_name: 2-Oxoglutarate; L-Kynurenine

substrate: 2-Oxoglutarate; L-Kynurenine


substrate_name: Asparagine

substrate: Asparagine


substrate_name: Serine

substrate: Serine


substrate_name: Alanine

substrate: Alanine


substrate_name: methionine

substrate: methionine


substrate_name: Aminobutyrate

substrate: Aminobutyrate


smiles: CCC(C)C(=O)C(=O)O


smiles: CCCCC(=O)C(=O)[O-]


smiles: CCCCC(=O)C(=O)O


smiles: O=C(O)C(=O)CS


substrate_name: Glyoxylate

smiles: O=CC(=O)O

substrate: Glyoxylate


substrate_name: L-Leucine

substrate: L-Leucine


substrate_name: L-Alanine

substrate: L-Alanine


substrate_name: hydroxyphenylpyruvate

substrate: hydroxyphenylpyruvate


substrate_name: glyoxylate

substrate: glyoxylate


substrate_name: Mercaptopyruvate

substrate: Mercaptopyruvate


substrate_name: 2-oxocaproic acid

substrate: 2-oxocaproic acid


substrate_name: phenylpyruvate

substrate: phenylpyruvate


substrate_name: pyruvate

smiles: CC(=O)C(=O)O

substrate: pyruvate


substrate_name: alpha-oxo-gamma-methiol-butyric acid

smiles: CSCCC(=O)C(=O)O

substrate: alpha-oxo-gamma-methiol-butyric acid


substrate_name: 2-oxoisocaproic acid

substrate: 2-oxoisocaproic acid


substrate_name: L-kynurenine

substrate: L-kynurenine


substrate_name: L-Methionine

substrate: L-Methionine


substrate_name: L-Phenylalanine

substrate: L-Phenylalanine


substrate_name: oxaloacetate

substrate: oxaloacetate


substrate_name: L-serine

substrate: L-serine


substrate_name: L-alanine

substrate: L-alanine


substrate_name: L-tyrosine

substrate: L-tyrosine


smiles: CCC(=O)C(=O)O


substrate_name: L-Cysteine

substrate: L-Cysteine


substrate_name: L-glutamine

substrate: L-glutamine


substrate_name: L-Asparagine

substrate: L-Asparagine


substrate_name: L-histidine

substrate: L-histidine


substrate_name: glyoxylate

substrate: glyoxylate


substrate_name: Mercaptopyruvate

substrate: Mercaptopyruvate


substrate_name: phenylpyruvate

substrate: phenylpyruvate


substrate_name: 2-oxocaproic acid

substrate: 2-oxocaproic acid


substrate_name: pyruvate

substrate: pyruvate


substrate_name: hydroxyphenylpyruvate

substrate: hydroxyphenylpyruvate


substrate_name: Pyruvate; L-Kynurenine

substrate: Pyruvate; L-Kynurenine


substrate_name: 2-Oxoglutarate; L-Kynurenine

substrate: 2-Oxoglutarate; L-Kynurenine


organism: Arabidopsis thaliana

substrate_name: UTP; D-Glucose 1-phosphate

proof_lines:
pyrophosphorolysis
UDP-glucose synthesis
Raw SKiD Env: Temp=-----, pH=-----

substrate: UTP; D-Glucose 1-phosphate


substrate_name: UTP; alpha-D-Glucose 1-phosphate

substrate: UTP; alpha-D-Glucose 1-phosphate


organism: Bos taurus


organism: Caenorhabditis elegans

substrate_name: alpha-D-glucose 1-phosphate

substrate: alpha-D-glucose 1-phosphate


substrate_name: dTTP

substrate: dTTP


substrate_name: UTP

substrate: UTP


organism: Cucumis melo

substrate_name: Diphosphate; UDPglucose

substrate: Diphosphate; UDPglucose


organism: Escherichia coli


substrate_name: D-Glucose 1-phosphate; UTP

substrate: D-Glucose 1-phosphate; UTP


organism: Synechocystis sp.


organism: Thermocrispum agreste


proof_lines:
pH 7.5, 37°C, recombinant enzyme
Raw SKiD Env: Temp=37°C, pH=7.5
Raw SKiD Env: Temp=37°C, pH=7.8


organism: Hordeum vulgare


organism: Nostoc sp.

substrate_name: diphosphate

substrate: diphosphate


organism: Solanum tuberosum


organism: Sulfurisphaera tokodaii


organism: Drosophila melanogaster

substrate_name: tryptamine (for the enzyme without substrate bound to the regulatory site and with acetyl CoA as cosubstrate)

source_db: uniprot

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

assay_conditions_summary: pH 8; cofactor CoA

proof_lines: kcat is 1.3 sec(-1) for acetyl-CoA (at pH 8 and with serotonin as cosubstrate) (PubMed:24444601). kcat is 0.33 sec(-1) for butanoyl-CoA (at pH 8 and with serotonin as cosubstrate) (PubMed:24444601). kcat is 0.16 sec(-1) for palmitoyl-CoA (a

source: uniprot


substrate_name: tryptamine (in transfected COS-7 cell extracts)

assay_conditions_summary: pH 7.2; cofactor CoA

proof_lines: kcat is 21.9 sec(-1) with tryptamine and acetyl-CoA as substrate (at pH 7.2).

proof_lines: kcat is 19 sec(-1) for tyramine (at pH 8 and with acetyl-CoA as cosubstrate) (PubMed:25406072). kcat is 13 sec(-1) for octapamine (at pH 8 and with acetyl-CoA as cosubstrate) (PubMed:25406072). kcat is 17.6 sec(-1) for dopamine (at pH 8 and


proof_lines: kcat is 206 min(-1) for cysteine (at 45 degrees Celsius, at pH 8.5 and with ketobutyrate as cosubstrate) (PubMed:15556614). kcat is 139 min(-1) for tyrosine (at 45 degrees Celsius, at pH 8.5 and with ketobutyrate as cosubstrate) (PubMed:155


organism: Bacillus subtilis (strain 168)

proof_lines: kcat is 1.06 sec(-1) with glucose-1-phosphate as substrate. kcat is 1.04 sec(-1) with UTP as substrate.


proof_lines: kcat is 0.264 sec(-1) with glucose-1-phosphate as substrate. kcat is 0.293 sec(-1) with UTP as substrate.


organism: Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)

proof_lines: kcat is 69.7 sec(-1) for GalN-1-P acetyltransferase activity. kcat is 123.2 sec(-1) for GlcN-1-P acetyltransferase activity.


organism: Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

substrate_name: UTP (at pH 7.5 and 37 degrees Celsius)

proof_lines: kcat is 28 sec(-1) (at pH 7.5 and 37 degrees Celsius).

substrate: UTP (at pH 7.5 and 37 degrees Celsius)


organism: Sus scrofa

substrate_name: L-carnitine

proof_lines: E17D mutant protein, mitochondrial preparation

substrate: L-carnitine


organism: Pyrococcus horikoshii

proof_lines:
0.1 mM L-kynurenine, pH 7.5, 25°C
pH 7.5, 25°C


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: D-Ala

proof_lines:
alr encoded enzyme
dadB encoded enzyme
alr gene encoded

substrate: D-Ala


organism: Staphylococcus aureus

substrate_name: L-alanine

proof_lines: pH and temperature not specified in the publication

substrate: L-alanine


organism: Streptomyces lavendulae

substrate_name: D-serine

substrate: D-serine


organism: Thalassiosira sp.

substrate_name: D-alanine

substrate: D-alanine


enzyme_display_name: chitinase

organism: Serratia marcescens

enzyme: chitinase


substrate_name: H2O; Chitobiose

substrate: H2O; Chitobiose


organism: Trichoderma harzianum

substrate_name: H2O; Chitin

substrate: H2O; Chitin


source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0


organism: Zea mays

substrate_name: H2O; Tetra-N-acetylchitotetraose

substrate: H2O; Tetra-N-acetylchitotetraose


organism: Saccharolobus solfataricus

substrate_name: amylopectin

proof_lines: pH 6.5, 75°C, calculated as microMol maltose released from the substrate, in absence of DMSO

proof_lines: pH 5.5, 75°C, calculated as microMol maltose released from the substrate, in presence of DMSO

proof_lines: pH 5.5, 75°C, calculated as microMol maltose released from the substrate, in absence of DMSO

substrate: amylopectin


organism: Aspergillus fumigatus


organism: Bacillus licheniformis


organism: Bacillus thuringiensis


organism: Gallus gallus


organism: Homo sapiens

proof_lines:
mutant N45D/D47N/R61M, pH 7.0, 37°C
mutant N45D/D47N/R61M, pH 4.6, 37°C
mutant N45D/D47N/R61M, pH 2.2, 37°C


organism: Listeria innocua


organism: Macaca fascicularis

substrate_name: 4-methylumbelliferyl chitobioside

substrate: 4-methylumbelliferyl chitobioside


organism: Ostrinia furnacalis


proof_lines:
domain ChtII-C1, at pH 6.0 and 30°C
domain ChtII-C2, at pH 6.0 and 30°C
domain ChtII-C1C2, at pH 6.0 and 30°C


organism: Saccharomyces cerevisiae


organism: Schistocerca gregaria


organism: Arabidopsis thaliana

substrate_name: Phosphoenolpyruvate; H2O; CO2

substrate: Phosphoenolpyruvate; H2O; CO2


organism: Brassica napus


organism: Corynebacterium glutamicum

substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


organism: Escherichia coli


organism: Flaveria pringlei


organism: Flaveria trinervia


organism: Methanothermobacter thermautotrophicus


organism: Sorghum bicolor


substrate_name: Phosphate; Oxaloacetate

substrate: Phosphate; Oxaloacetate


organism: Zea mays


substrate_name: CO2; Phosphoenolpyruvate; H2O

substrate: CO2; Phosphoenolpyruvate; H2O


enzyme_display_name: chloramphenicol O-acetyltransferase

organism: Unknown

substrate_name: chloramphenicol

enzyme: chloramphenicol O-acetyltransferase

substrate: chloramphenicol


organism: Bombyx mori

substrate_name: Glycolchitin; H2O

proof_lines:
isoenzyme 1
isoenzyme 2

substrate: Glycolchitin; H2O


substrate_name: glycogen phosphorylase limit dextrin

substrate: glycogen phosphorylase limit dextrin


proof_lines: dephosphorylated isozyme PPC1, at pH 7.3


proof_lines:
pH 8.0
isoenzyme PC-I
isoenzyme PC-II


organism: Homo sapiens

substrate_name: aminoadipate

substrate: aminoadipate


organism: Mus musculus

substrate_name: glutamine

substrate: glutamine


organism: Rattus norvegicus

substrate_name: L-kynurenine (with 2-oxoglutarate as cosubstrate)


substrate_name: L-kynurenine

substrate: L-kynurenine


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: tryptophan

substrate: tryptophan


substrate_name: glucose 1-phosphate

substrate: glucose 1-phosphate


substrate_name: MgUTP

substrate: MgUTP


organism: Aspergillus fumigatus


organism: Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)

substrate_name: SR-linked chitopentaose

substrate: SR-linked chitopentaose


organism: Glutamicibacter uratoxydans

substrate_name: Colloidal Chitin

sequence: ADGTITFNGKVTDK

wild_type_sequence: ADGTITFNGKVTDK

substrate: Colloidal Chitin


substrate_name: carnitine

substrate: carnitine


substrate_name: hexadecanoyl-CoA

substrate: hexadecanoyl-CoA


organism: Arabidopsis thaliana

substrate_name: serotonin

substrate: serotonin


substrate_name: tryptamine

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

substrate: tryptamine


organism: Oryza sativa subsp. japonica

substrate_name: serotonin (at 55 degrees Celsius)

substrate: serotonin (at 55 degrees Celsius)


data_origin: experimental


organism: Ovis aries

substrate_name: acetyl-CoA

substrate: acetyl-CoA


substrate_name: Unknown

substrate: Unknown


organism: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)

substrate_name: phosphoenolpyruvate

verification_status: corrected

substrate: phosphoenolpyruvate

status: corrected


organism: Paenibacillus xylanexedens

substrate_name: colloidal chitin

proof_lines: kcat is 0.08 sec(-1) with colloidal chitin as substrate.

substrate: colloidal chitin


organism: Punica granatum

proof_lines: kcat/KM is 0.119 M(-1)sec(-1) for native protein, 0.130 M(-1)sec(-1) for native protein with 10 mM Ca(2+) and 0.116 M(-1)sec(-1) for EDTA-treated protein, using 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside (4-MU(GlcNAc)(3)) a


organism: Yersinia entomophaga

proof_lines: In the intact toxin complex the combined chitinase activity of Chi1 and Chi2 is slightly reduced.


organism: Zea mays

substrate_name: tetra-N-acetylchitotetraose (at 37 degrees Celsius and pH 6)

assay_conditions_summary: pH 6

proof_lines: kcat is 56.27 sec(-1) with tetra-N-acetylchitotetraose as substrate (at 37 degrees Celsius and pH 6). kcat is 55.67 sec(-1) with tetra-N-acetylchitotetraose as substrate (at 37 degrees Celsius and pH 4). kcat is 69.01 sec(-1) with tetra-N-a


organism: Pseudomonas aeruginosa


substrate_name: chloramphenicol

substrate: chloramphenicol


organism: Medicago truncatula


organism: Metarhizium anisopliae

substrate_name: p-nitrophenyl beta-N-diacetylchitobiose

substrate: p-nitrophenyl beta-N-diacetylchitobiose


organism: Streptomyces violaceusniger

sequence: GDGTGPGPGP

wild_type_sequence: GDGTGPGPGP


organism: Trichoderma harzianum


substrate_name: PEP (Phospho-PPC1 at pH 7.3 and 25 degrees Celsius)


organism: Flaveria trinervia


organism: Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)


organism: Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)


organism: Proteus mirabilis


proof_lines:
enzyme variant CATA1
enzyme variant CATB1
enzyme variant CATB5


organism: Staphylococcus aureus


proof_lines:
enzyme variant D
enzyme variant C


organism: Saccharomyces cerevisiae

substrate_name: 6-O-alpha-D-glucosyl cyclomaltoheptaose

substrate: 6-O-alpha-D-glucosyl cyclomaltoheptaose


organism: Agrobacterium tumefaciens


organism: Bacillus pumilus


organism: Clostridium perfringens


organism: Escherichia coli


substrate_name: isobutanol

substrate: isobutanol


proof_lines: pH 7.0, 60°C, recombinant wild-type enzyme


proof_lines:
enzyme variant CAT III
enzyme variant CAT I
enzyme variant CATIII


organism: Flavobacterium sp.


organism: Haemophilus parainfluenzae


organism: Morganella morganii


substrate_name: amylopectin beta-dextrin

proof_lines:
phi-dextrin from shellfish glycogen
beta-dextrin from shellfish glycogen

substrate: amylopectin beta-dextrin


proof_lines:
enzyme from plasmid pSCS6
enzyme from plasmid pSCS7


organism: Staphylococcus epidermidis


organism: Staphylococcus sp.


organism: Streptococcus agalactiae


organism: Nostoc punctiforme

substrate_name: maltodecaose

assay_conditions_summary: pH 7.5; 303.15 K; buffer 50 mM sodium phosphate

proof_lines: in 50 mM sodium phosphate buffer (pH 7.5) at 30°C

substrate: maltodecaose


organism: Oryctolagus cuniculus

substrate_name: glycogen phosphorylase limit dextrin

proof_lines:
phi-dextrin
phi-dextrin from shellfish glycogen
branched pentasaccharide B5, pH 6.6

substrate: glycogen phosphorylase limit dextrin


organism: Saccharolobus shibatae

assay_conditions_summary: pH 5.5; 343.15 K; buffer 50 mM sodium acetate

proof_lines: at 70°C in 50 mM sodium acetate buffer (pH 5.5)


organism: Saccharolobus solfataricus

substrate_name: maize amylopectin

proof_lines: pH 5.0, 70°C, recombinant wild-type enzyme

substrate: maize amylopectin


organism: Streptococcus pneumoniae


organism: Streptomyces acrimycini


organism: Vibrio anguillarum

assay_conditions_summary: cofactor CoA


organism: Tolypocladium inflatum

substrate_name: L-alanine

substrate: L-alanine


organism: Salmonella enterica

substrate_name: ATP; Cob(I)alamin

assay_conditions_summary: pH 7; 310.15 K; cofactor ATP

proof_lines:
pH 7.0, 37°C, recombinant enzyme
at pH 7.0 and 37°C

substrate: ATP; Cob(I)alamin


organism: Suaeda eltonica

assay_conditions_summary: pH 8

proof_lines: leaves are collected after 5 h into the light, pH: 8.0

proof_lines: leaves are collected after 5 h into the dark, pH: 8.0


organism: Umbilicus rupestris

proof_lines: pH 8.2, enzyme from water stressed plants

proof_lines: pH 7.2, enzyme from water stressed plants


organism: Xylosalsola richteri


organism: Bienertia sinuspersici

proof_lines: leaves are collected after 5 h in the dark, pH: 8.0

proof_lines: leaves are collected after 5 h in the light, pH: 8.0


organism: Corynebacterium glutamicum

proof_lines: protein with N-terminal His-tag, pH 7.5, 30Â°C

proof_lines: protein with C-terminal His-tag, pH 7.5, 30Â°C


organism: Ricinus communis

proof_lines:
mutant enzyme S425A, at pH 8.0 and 25°C
mutant enzyme S425A, at pH 7.3 and 25°C


organism: Suaeda aralocaspica


enzyme_display_name: inorganic diphosphatase

organism: Trichinella spiralis

substrate_name: diphosphate

assay_conditions_summary: pH 7.5; 328.15 K; cofactor ATP

enzyme: inorganic diphosphatase

substrate: diphosphate


organism: Trypanosoma cruzi

substrate_name: Triphosphate

proof_lines:
with 3 mM Co2+, at pH 6.5 and 30°C
with 3 mM Zn2+, at pH 6.5 and 30°C
with 3 mM Mg2+, at pH 8.5 and 30°C

substrate: Triphosphate


organism: Vibrio cholerae serotype O1

proof_lines: in the presence of 10 mM Mg2+, in 50 mM Mes-NaOH, at pH 6.5 and 25°C

proof_lines: in the presence of 0.005 mM Mn2+, in 50 mM HEPES-NaOH, at pH 7.5 and 25°C

proof_lines: in the presence of 5 mM Mg2+, in 50 mM Tris-HCl, at pH 9.0 and 25°C


enzyme_display_name: myeloperoxidase

organism: Homo sapiens

enzyme: myeloperoxidase


organism: Rattus norvegicus

substrate_name: H2O; Diphosphate

substrate: H2O; Diphosphate


organism: Rhodopseudomonas palustris

substrate_name: Diphosphate; H2O

substrate: Diphosphate; H2O


organism: Sorghum bicolor


organism: Bos taurus

substrate_name: SCN-

substrate: SCN-


substrate_name: benzyl methyl sulfide

assay_conditions_summary: pH 5; 293.15 K; buffer 50 mM acetate

proof_lines: in the absence of NaCl, in 50 mM acetate buffer, pH 5.0, at 20°C

proof_lines: in the presence of 100 mM NaCl, in 50 mM acetate buffer, pH 5.0, at 20°C

proof_lines: at 21°C in 50 mM sodium phosphate buffer, pH 7.4

substrate: benzyl methyl sulfide


organism: Oryctolagus cuniculus

substrate_name: UTP; D-Glucose 1-phosphate

substrate: UTP; D-Glucose 1-phosphate


organism: Saccharomyces cerevisiae


substrate_name: D-Glucose 1-phosphate; UTP

substrate: D-Glucose 1-phosphate; UTP


enzyme_display_name: protein N-terminal methyltransferase

substrate_name: N-terminal-SPKRIAKRRSPP

proof_lines: recombinant His6-tagged enzyme, pH 7.5, 37°C

proof_lines: recombinant detagged enzyme, pH 7.5, 37°C

proof_lines: pH and temperature not specified in the publication

enzyme: protein N-terminal methyltransferase

substrate: N-terminal-SPKRIAKRRSPP


proof_lines:
about, mutant W136F, pH 7.5, 37°C
above, mutant W136F, pH 7.5, 37°C


substrate_name: N-terminal-WPKRIA

substrate: N-terminal-WPKRIA


organism: Cucumis sativus

substrate_name: Phosphoenolpyruvate; H2O; CO2

proof_lines: pH 8.0, 25°C, enzyme from roots grown in absence of Fe2+

proof_lines: pH 8.0, 25°C, enzyme from roots grown in presence of Fe2+

substrate: Phosphoenolpyruvate; H2O; CO2


organism: Methanothermus sociabilis


proof_lines:
pH 8, PEPC1
pH 8, PEPC2
pH 7.3, PEPC1


enzyme_display_name: carbonyl reductase (NADPH)

organism: Cavia porcellus

substrate_name: 4-Nitroacetophenone; NADPH

assay_conditions_summary: cofactor NADPH

enzyme: carbonyl reductase (NADPH)

substrate: 4-Nitroacetophenone; NADPH


organism: Gallus gallus

substrate_name: H+; NADPH; Pyridine-4-aldehyde

substrate: H+; NADPH; Pyridine-4-aldehyde


substrate_name: H+; NADPH; Isatin

substrate: H+; NADPH; Isatin


substrate_name: NADPH; Pyridine-4-aldehyde; H+

substrate: NADPH; Pyridine-4-aldehyde; H+


organism: Canis familiaris

substrate_name: Palmitoyl-CoA; L-Carnitine

substrate: Palmitoyl-CoA; L-Carnitine


enzyme_display_name: non-specific serine/threonine protein kinase


enzyme_display_name: alcohol dehydrogenase (NADP+)

organism: Clostridium acetobutylicum

substrate_name: NADP+; 1-Butanol

assay_conditions_summary: cofactor NADP+

enzyme: alcohol dehydrogenase (NADP+)

substrate: NADP+; 1-Butanol


substrate_name: H+; D-Glucuronate; NADPH

substrate: H+; D-Glucuronate; NADPH


organism: Lactococcus lactis

substrate_name: H+; NADH; 2-Methylpropanal

assay_conditions_summary: cofactor NADH

substrate: H+; NADH; 2-Methylpropanal


substrate_name: NADPH; p-Nitrobenzaldehyde; H+

substrate: NADPH; p-Nitrobenzaldehyde; H+


substrate_name: NADPH; Acetaldehyde; H+

substrate: NADPH; Acetaldehyde; H+


enzyme_display_name: thymidine kinase

organism: Human herpesvirus 1

substrate_name: ATP; Thymidine

enzyme: thymidine kinase

substrate: ATP; Thymidine


organism: Mus musculus


substrate_name: ATP; 1-beta-D-Arabinofuranosylthymine

substrate: ATP; 1-beta-D-Arabinofuranosylthymine


organism: Vaccinia virus

substrate_name: VVTK; Deoxythymidine

substrate: VVTK; Deoxythymidine


organism: Arabidopsis thaliana

substrate_name: CRCK1; Calmodulin

enzyme: non-specific serine/threonine protein kinase

substrate: CRCK1; Calmodulin


organism: Danio rerio

substrate_name: ATP; Protein

substrate: ATP; Protein


organism: Opisthokonta

substrate_name: cdc2k; ATP

verification_status: unverified

substrate: cdc2k; ATP

status: unverified


organism: Acer pseudoplatanus

substrate_name: Diphosphate; UDPglucose

substrate: Diphosphate; UDPglucose


organism: Felis silvestris catus

substrate_name: D-Glucose 1-phosphate; UTP

substrate: D-Glucose 1-phosphate; UTP


enzyme_display_name: granzyme B

organism: Homo sapiens

proof_lines:
pH 7.4, 37°C, wild-type
wild-type, 37°C

enzyme: granzyme B


organism: Dioscorea opposita

substrate_name: H2O; Glycolchitin

substrate: H2O; Glycolchitin


organism: Pagrus major

substrate_name: Glycolchitin; H2O

substrate: Glycolchitin; H2O


organism: Todarodes pacificus


substrate_name: Boc-Ala-Ala-Asp-SBzl

substrate: Boc-Ala-Ala-Asp-SBzl


proof_lines:
pH 7.5, 23°C
mouse


enzyme_display_name: ADAMTS13 endopeptidase

substrate_name: VWF73 peptide

proof_lines: pH 6.0, 37°C, recombinant G662A mutant ADAMTS13

enzyme: ADAMTS13 endopeptidase

substrate: VWF73 peptide


mutation_type: deletion

proof_lines: pH 6.0, 37°C, recombinant ADAMTS13 6-amino-acid-deletion mutant


proof_lines: pH 6.0, 37°C, recombinant Y661A mutant ADAMTS13


proof_lines: pH 6.0, 37°C, recombinant R659A mutant ADAMTS13


proof_lines: pH 6.0, 37°C, recombinant E664A mutant ADAMTS13


proof_lines: pH 6.0, 37°C, recombinant E663A mutant ADAMTS13


proof_lines: pH 6.0, 37°C, recombinant R660A mutant ADAMTS13


proof_lines: mutant R568K/F592Y/R660K/Y661F/Y665F, pH not specified in the publication, temperature not specified in the publication


substrate_name: von Willebrand factor 115

proof_lines:
recombinant enzyme, at 37°C
pH 7.5, 25°C, recombinant enzyme
pH 7.5, 37°C, recombinant ADAMTS13

substrate: von Willebrand factor 115


substrate_name: FRET-VWF115 peptide

substrate: FRET-VWF115 peptide


proof_lines: wild type enzyme, in 20 mM Tris-HCl (pH 7.8), 150 mM NaCl, 5 mM CaCl2, at 37°C

proof_lines: pH 7.8, 37°C, recombinant wild-type enzyme

proof_lines: pH 6.0, 37°C, recombinant wild-type ADAMTS13


enzyme_display_name: fructose-bisphosphate aldolase

organism: Bos taurus

substrate_name: D-Fructose 1,6-bisphosphate

enzyme: fructose-bisphosphate aldolase

substrate: D-Fructose 1,6-bisphosphate


organism: Bubalus bubalis


organism: Ceratitis capitata


organism: Chara foetida


organism: Spinacia oleracea

substrate_name: Diphosphate; H2O

substrate: Diphosphate; H2O


organism: Streptococcus gordonii


enzyme_display_name: phosphoenolpyruvate carboxylase

organism: Acholeplasma laidlawii

substrate_name: Phosphoenolpyruvate; H2O; CO2

enzyme: phosphoenolpyruvate carboxylase

substrate: Phosphoenolpyruvate; H2O; CO2


organism: Flaveria trinervia


organism: Triticum aestivum


organism: Helix pomatia


organism: Lactococcus lactis subsp. lactis


organism: Micrococcus aerogenes


organism: Cyprinus carpio


organism: Entamoeba histolytica


organism: Escherichia coli


organism: Galdieria sulphuraria


organism: Saccharomyces cerevisiae

substrate_name: beta-D-Fructose 1,6-bisphosphate

substrate: beta-D-Fructose 1,6-bisphosphate


organism: Scomber japonicus


organism: Spermophilus lateralis


organism: Staphylococcus aureus

substrate_name: D-Fructose 1-phosphate

substrate: D-Fructose 1-phosphate


organism: Sus scrofa


organism: Ustilago maydis


organism: Vigna radiata


enzyme_display_name: glucose-6-phosphate isomerase

organism: Archaeoglobus fulgidus

substrate_name: D-Glucose 6-phosphate

enzyme: glucose-6-phosphate isomerase

substrate: D-Glucose 6-phosphate


organism: Bacillus caldotenax


organism: Conger conger

substrate_name: D-Fructose 6-phosphate

substrate: D-Fructose 6-phosphate


organism: Daphnia magna


organism: Fundulus heteroclitus


organism: Ictalurus punctatus


organism: Lactobacillus casei


organism: Methanosarcina mazei


organism: Oryctolagus cuniculus


organism: Pagrus major


organism: Rattus norvegicus


organism: Ricinus communis


organism: Spinacia oleracea


organism: Trypanosoma cruzi


substrate_name: Glucose 6-phosphate

substrate: Glucose 6-phosphate


organism: Pisum sativum


organism: Saccharomyces cerevisiae

substrate_name: alpha-D-Glucose 6-phosphate

substrate: alpha-D-Glucose 6-phosphate


organism: Salmonella typhimurium


enzyme_display_name: pancreatic ribonuclease

organism: Bos taurus

substrate_name: Uridine monophosphate-uridine; H2O

proof_lines: binds to the active site of the enzyme by anchoring two molecules connected to each other by hydrogen bonds and van der Waals interactions

enzyme: pancreatic ribonuclease

substrate: Uridine monophosphate-uridine; H2O


organism: Equus caballus

substrate_name: 2',3'-Cyclic UMP; H2O

substrate: 2',3'-Cyclic UMP; H2O


organism: Gallus gallus

substrate_name: tRNA

assay_conditions_summary: pH 7

substrate: tRNA


organism: Homo sapiens

substrate_name: poly(A)poly(U)

substrate: poly(A)poly(U)


substrate_name: poly (C)

proof_lines: mutant R4A/K6A/Q9E/D16G/S17N, 25°C, pH 5.0

substrate: poly (C)


proof_lines:
wild-type, 25°C, pH 5.0
wild type enzyme


substrate_name: tRNA; H2O

substrate: tRNA; H2O


substrate_name: cyclic 2',3'-cytidine monophosphate

substrate: cyclic 2',3'-cytidine monophosphate


organism: Lithobates pipiens

substrate_name: Unknown

substrate: Unknown


organism: Macaca fascicularis


organism: Pongo pygmaeus


organism: Salmo salar


organism: Sus scrofa


substrate_name: cytidine-2',3'-cyclic monophosphate

substrate: cytidine-2',3'-cyclic monophosphate


proof_lines:
wild-type, pH 5.5, 25°C
wild-type, pH 7.0
wild-type, pH7.0


proof_lines:
mutant K7H/R10H, pH 5.5, 25°C
mutant K7H/R10H, pH 7.0


substrate_name: pentacytidylic acid

substrate: pentacytidylic acid


substrate_name: poly(A)-poly(U)

substrate: poly(A)-poly(U)


substrate_name: poly(C)

substrate: poly(C)


proof_lines:
wild type enzyme, pH 5.5, 25°C
wild type and mutant enzyme


proof_lines:
wild-type, pH 7.5, 37°C
pH 7.5, 37°C, wild-type enzyme


substrate_name: RNA

proof_lines:
pH 7.0, 37°C
pH 5.5, 25ºC, comercial enzyme
pancreas

substrate: RNA


proof_lines:
PE3I1 variant, pH 5.5, 25ºC
PE3 variant, pH 5.5, 25ºC
PE3I2 variant, pH 5.5, 25ºC


substrate_name: cyclic 2',3'-CMP

substrate: cyclic 2',3'-CMP


organism: Balaenoptera physalus


substrate_name: H2O; 2',3'-Cyclic CMP

substrate: H2O; 2',3'-Cyclic CMP


substrate_name: SSKRAKAKTTKKRP

proof_lines: recombinant NRMT1, in presence of NRMT2 catalytically inactive V224L mutant, pH and temperature not specified in the publication

substrate: SSKRAKAKTTKKRP


substrate_name: S-adenosyl-L-methionine

proof_lines: with RCC1, pH and temperature not specified in the publication

proof_lines: with (E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine, pH and temperature not specified in the publication

proof_lines: with S-adenosyl-L-methionine, pH and temperature not specified in the publication

substrate: S-adenosyl-L-methionine


substrate_name: Ac-IEPD-p-nitroanilide

substrate: Ac-IEPD-p-nitroanilide


substrate_name: beta-glycan

substrate: beta-glycan


organism: Rattus norvegicus


proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


organism: Aotus trivirgatus


assay_conditions_summary: cofactor ATP


organism: Aquifex aeolicus

substrate_name: ATP

substrate: ATP


organism: Archaeoglobus fulgidus

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP


substrate_name: Peptide

substrate: Peptide


assay_conditions_summary: pH 7.4; 295.15 K; cofactor ATP


substrate_name: ATP; alpha-Casein

substrate: ATP; alpha-Casein


proof_lines: pH and temperature not specified in the publication


substrate_name: Lys-Lys-Phe-Asn-Arg-Thr-Leu-Ser-Val-Ala

substrate: Lys-Lys-Phe-Asn-Arg-Thr-Leu-Ser-Val-Ala


organism: Mycobacterium tuberculosis

substrate_name: EmbR; ATP

substrate: EmbR; ATP


substrate_name: ATP; Protein tyrosine

substrate: ATP; Protein tyrosine


enzyme_display_name: 4-hydroxyphenylpyruvate dioxygenase

organism: Mus musculus

substrate_name: 4-Hydroxyphenylpyruvate; O2

enzyme: 4-hydroxyphenylpyruvate dioxygenase

substrate: 4-Hydroxyphenylpyruvate; O2


organism: Picrophilus torridus


substrate_name: O2; 4-Hydroxyphenylpyruvate

substrate: O2; 4-Hydroxyphenylpyruvate


organism: Sphingobium sp.


organism: Streptomyces avermitilis


smiles: O=O


enzyme_display_name: phenylglycine N-acetyltransferase

organism: Chryseobacterium sp.

substrate_name: L-2-phenylglycine methyl ester

enzyme: phenylglycine N-acetyltransferase

substrate: L-2-phenylglycine methyl ester


organism: Chryseobacterium sp. 5-3B


organism: Arabidopsis thaliana


proof_lines: recombinant His-tagged enzyme, pH 7.0, 30°C

proof_lines: 30°C, pH not specified in the publication


organism: Homo sapiens


proof_lines: pH 7.0, 30°C, recombinant His-tagged enzyme


assay_conditions_summary: pH 7.5; 303.15 K; cofactor CoA


enzyme_display_name: tetraacyldisaccharide 4'-kinase

assay_conditions_summary: pH 8.5; 303.15 K; cofactor ATP

enzyme: tetraacyldisaccharide 4'-kinase


substrate_name: acetyl-CoA

substrate: acetyl-CoA


substrate_name: synthetic substrate

substrate: synthetic substrate


organism: Badhamia polycephala

substrate_name: actin-fragmin complex

substrate: actin-fragmin complex


organism: Bos taurus


organism: Caenorhabditis elegans

substrate_name: manganese/ATP

substrate: manganese/ATP


substrate_name: ATP (in the presence of 10 mM magnesium chloride)


substrate_name: manganese ions

substrate: manganese ions


substrate_name: phosphoenolpyruvate (at 32 degrees Celsius, pH 8.0)

assay_conditions_summary: cofactor ADP


substrate_name: ATP (for purified and in vitro activated AKT2)


substrate_name: ATP (for purified and in vitro activated AKT3)


substrate_name: ATP (for purified and in vitro activated AKT1)


substrate_name: myosin (isoform 2)

substrate: myosin (isoform 2)


proof_lines: pH 8.5, 30°C, recombinant wild-type enzyme


enzyme_display_name: lysine carboxypeptidase

substrate_name: fibrinopeptide alphaArg96-104

enzyme: lysine carboxypeptidase

substrate: fibrinopeptide alphaArg96-104


proof_lines: kcat is 2.0 sec(-1) with 3-(4-hydroxyphenyl)pyruvate as substrate.


substrate_name: ATP

proof_lines: Kinetics of autophosphorylation assay were measured, rather than phosphorylation of an exogenous substrate.


substrate_name: alpha-casein

proof_lines: Maximal velocity nearly identical for the reactions with alpha-casein and PER2 peptide.

substrate: alpha-casein


substrate_name: casein

substrate: casein


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: pNPP

proof_lines: kcat is 14.2 min(-1) for pNPP hydrolysis.

substrate: pNPP


organism: Plasmodium falciparum (isolate 3D7)

substrate_name: ATP (at 30 degrees Celsius)

proof_lines: kcat is 0.002 sec(-1) with ATP as substrate (at 30 degrees Celsius).

substrate: ATP (at 30 degrees Celsius)


organism: Plasmodium falciparum (isolate Dd2)

substrate_name: ATP (at pH 7.5 and 37 degrees Celsius)

assay_conditions_summary: pH 7.5; cofactor ATP

proof_lines: kcat is 1550 sec(-1) with ATP as substrate (at pH 7.5 and 37 degrees Celsius).

substrate: ATP (at pH 7.5 and 37 degrees Celsius)


organism: Tribolium castaneum

substrate_name: Drp1 (at 30 degrees Celsius)

proof_lines: kcat is 4.6 sec(-1) for phosphorylation of Drp1 (at pH 7.5 and 30 degrees Celsius) (PubMed:32484300). kcat is 20.9 sec(-1) for phosphorylation of ubiquitin (at pH 7.5 and 30 degrees Celsius) (PubMed:32484300). kcat is 18 min(-1) for phospho

substrate: Drp1 (at 30 degrees Celsius)


enzyme_display_name: ribonuclease III

organism: Escherichia coli

substrate_name: R1.1 RNA

assay_conditions_summary: pH 7.5; 310.15 K; buffer containing 10 mM Mg2+, mutant enzyme E65A

proof_lines: pH 7.5, 37°C, buffer containing 10 mM Mg2+, mutant enzyme E65A

enzyme: ribonuclease III

substrate: R1.1 RNA


assay_conditions_summary: pH 7.5; 310.15 K; buffer containing 10 mM Mg2+, mutant enzyme E100A

proof_lines: pH 7.5, 37°C, buffer containing 10 mM Mg2+, mutant enzyme E100A


assay_conditions_summary: pH 7.5; 310.15 K; buffer containing 10 mM Mg2+, mutant enzyme E38A

proof_lines: pH 7.5, 37°C, buffer containing 10 mM Mg2+, mutant enzyme E38A


assay_conditions_summary: pH 7.5; 310.15 K; buffer containing 10 mM Mg2+, mutant enzyme D114A

proof_lines: pH 7.5, 37°C, buffer containing 10 mM Mg2+, mutant enzyme D114A


assay_conditions_summary: pH 7.5; 310.15 K; buffer containing 10 mM Mg2+, wild-type enzyme

proof_lines: pH 7.5, 37°C, buffer containing 10 mM Mg2+, wild-type enzyme

proof_lines: recombinant wild-type enzyme, 37°C, in presence of 5 mM Mg2+

proof_lines: wild-type enzyme, pH 7.5, 37°C, in presence of Mg2+


assay_conditions_summary: pH 7.5; 310.15 K; buffer containing 10 mM Mg2+, mutant enzyme E41A

proof_lines: pH 7.5, 37°C, buffer containing 10 mM Mg2+, mutant enzyme E41A


substrate: ATP


enzyme_display_name: acyl-homoserine-lactone synthase

organism: Acinetobacter baumannii

substrate_name: Unknown

proof_lines: value calculated in silico, pH not specified in the publication, temperature not specified in the publication

enzyme: acyl-homoserine-lactone synthase

substrate: Unknown


organism: Aliivibrio fischeri

substrate_name: octanoyl-[acyl-carrier protein]

assay_conditions_summary: pH 8.5; 298.15 K; cofactor CoA

proof_lines: pH 8.5, 25°C, recombinant MBP-AinS, with octanoyl-[acyl-carrier-protein]

substrate: octanoyl-[acyl-carrier protein]


organism: Pseudomonas aeruginosa

substrate_name: butyryl-CoA

assay_conditions_summary: pH 8.8; 298.15 K; cofactor CoA

proof_lines:
pH 8.8, 25°C, recombinant enzyme
pH 7.8, 37°C, recombinant RhlI

substrate: butyryl-CoA


organism: Serratia fonticola

substrate_name: hexanoyl-[acyl-carrier protein]

assay_conditions_summary: cofactor CoA

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: hexanoyl-[acyl-carrier protein]


organism: Saccharomyces cerevisiae

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

proof_lines:
at pH 7.5 and 30°C
pH 7.5, 30°C


substrate_name: UDP-GlcNAc

substrate: UDP-GlcNAc


organism: Ceratitis capitata

substrate_name: methyl-alpha-D-mannoside

substrate: methyl-alpha-D-mannoside


substrate_name: methyl alpha-D-mannopyranoside

proof_lines: pH 7.4, 37°C, alpha2beta2 GlcNAc-1-phosphotransferase

proof_lines: pH 7.4, 37°C, alpha2beta2gamma2 GlcNAc-1-phosphotransferase

substrate: methyl alpha-D-mannopyranoside


organism: Rattus norvegicus


assay_conditions_summary: cofactor ATP

proof_lines: in vivo PDK1-activated mutant DELTA399-502/T389E (His6-S6K1aII(DELTA AID)-T389E)

proof_lines: in vitro PDK1-activated mutant DELTA399-502/T389E (His6-S6K1aII(DELTA AID)-T389E)


organism: Mus musculus

substrate_name: myelin basic protein

substrate: myelin basic protein


organism: Saccharolobus solfataricus

substrate_name: KKRAARATSNVFA

assay_conditions_summary: pH 6.5; 298.15 K; cofactor ATP

proof_lines: pH 6.5, 25°C, presence of poly(Glu4:Tyr)

substrate: KKRAARATSNVFA


proof_lines: recombinant enzyme mutant RNase III[DELTAdsRBD], 37°C, in presence of 5 mM Mn2+

proof_lines: recombinant enzyme mutant RNase III[DELTAdsRBD], 37°C, in presence of 25 mM Mg2+


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: poly(IC)

substrate: poly(IC)


organism: Aquifex aeolicus


organism: Drosophila melanogaster

substrate_name: 515 bp dsRNA

proof_lines: wild-type Dicer-2 in presence of Loquacious-PB at equimolar level, pH 7.4, 25°C

proof_lines: wild-type Dicer-2 in presence of protein R2D2 at equimolar level, pH 7.4, 25°C

substrate: 515 bp dsRNA


proof_lines: mutant E117D, pH 7.5, 37°C, in presence of Mn2+


substrate_name: RNA

proof_lines: recombinant His-tagged enzyme, pH 8.0, 35°C

substrate: RNA


organism: Homo sapiens

substrate_name: fibrinopeptide gammaLys54-62

substrate: fibrinopeptide gammaLys54-62


substrate_name: hippuryl-L-Arg

substrate: hippuryl-L-Arg


organism: Sus scrofa

substrate_name: Hippuryl-L-argininic acid

substrate: Hippuryl-L-argininic acid


organism: Echinochloa crus-galli

substrate_name: O2; 4-Hydroxyphenylpyruvate

substrate: O2; 4-Hydroxyphenylpyruvate


substrate_name: ATP; Protein

proof_lines: values for inhibition at the PH-domain of PKBalpha are obtained using competition with biotinylated diC8-phosphatidylinositol 3,4-bisphosphate

substrate: ATP; Protein


enzyme_display_name: alanine racemase

organism: Acidiphilium organovorum

substrate_name: D-Ala

assay_conditions_summary: pH 9

enzyme: alanine racemase

substrate: D-Ala


organism: Aquifex pyrophilus


organism: Bacillus anthracis

substrate_name: L-alanine

substrate: L-alanine


organism: Cellana grata


organism: Danio rerio


organism: Oryctolagus cuniculus


proof_lines: kcat is 0.03 min(-1) for dsRNA consumption (PubMed:21419681). kcat is 0.03 min(-1) for dsRNA consumption (in the presence of r2d2) (PubMed:21419681). kcat is 0.03 min(-1) for dsRNA consumption (in the presence of loqs isoform PD) (PubMed:21


organism: Aeromonas hydrophila

substrate_name: butyryl-[acyl-carrier protein]

substrate: butyryl-[acyl-carrier protein]


organism: Mesorhizobium sp. ORS 3359

substrate_name: isobutanoyl-[ACP]

substrate: isobutanoyl-[ACP]


organism: Escherichia coli

substrate_name: R1.1 RNA

proof_lines: recombinant phosphorylated enzyme, pH 8.0, 37°C

proof_lines: recombinant non-phosphorylated enzyme, pH 8.0, 37°C

substrate: R1.1 RNA


organism: Saccharomyces cerevisiae


organism: Rattus norvegicus

substrate_name: eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) (in absence of DELE1 and in absence of hemin)


organism: Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

substrate_name: ATP during hexamerization

substrate: ATP during hexamerization


organism: Toxoplasma gondii

substrate_name: ATP (at pH 7.5 and 30 degrees Celsius)

substrate: ATP (at pH 7.5 and 30 degrees Celsius)


organism: Aquifex aeolicus (strain VF5)

substrate_name: 16S-u-hp RNA

substrate: 16S-u-hp RNA


organism: Homo sapiens

substrate_name: ATP (at pH 7.5)

substrate: ATP (at pH 7.5)


substrate_name: IDP

substrate: IDP


substrate_name: ATP

substrate: ATP


substrate_name: RRKDLHDDEEDEAMSIYpA peptide

substrate: RRKDLHDDEEDEAMSIYpA peptide


substrate_name: manganese/ATP

substrate: manganese/ATP


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: EmbR

substrate: EmbR


substrate_name: GarA

substrate: GarA


organism: Oryza sativa subsp. indica


organism: Plasmodium falciparum (isolate 3D7)

substrate_name: MTIP (at 30 degrees Celsius)

substrate: MTIP (at 30 degrees Celsius)


enzyme_display_name: glutathione-disulfide reductase

organism: Mus musculus

substrate_name: H+; NADPH; Oxidized glutathione

enzyme: glutathione-disulfide reductase

substrate: H+; NADPH; Oxidized glutathione


organism: Populus trichocarpa

substrate_name: NADPH

assay_conditions_summary: pH 7.6; 295.15 K; cofactor NADPH

substrate: NADPH


substrate_name: glutathione disulfide

substrate: glutathione disulfide


assay_conditions_summary: pH 7.6; 303.15 K; cofactor NADPH


substrate_name: histone H3 N6,N6,N6-trimethyl-L-lysine9

proof_lines: KDM4C, pH and temperature not specified in the publication

substrate: histone H3 N6,N6,N6-trimethyl-L-lysine9


substrate_name: histone H3 N6,N6-dimethyl-L-lysine9

proof_lines: KDM4B, pH and temperature not specified in the publication

substrate: histone H3 N6,N6-dimethyl-L-lysine9


proof_lines: KDM4A, pH and temperature not specified in the publication


proof_lines: KDM4D, pH and temperature not specified in the publication


proof_lines: KDM4E, pH and temperature not specified in the publication


substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication

substrate: Unknown


enzyme_display_name: biphenyl 2,3-dioxygenase

organism: Paraburkholderia xenovorans

substrate_name: biphenyl

proof_lines: mutant enzyme S283M, at 37°C, pH not specified in the publication

enzyme: biphenyl 2,3-dioxygenase

substrate: biphenyl


proof_lines: wild type enzyme, at 37°C, pH not specified in the publication


enzyme_display_name: alkane 1-monooxygenase

organism: Pseudomonas putida

enzyme: alkane 1-monooxygenase


enzyme_display_name: putidaredoxin--NAD(+) reductase

enzyme: putidaredoxin--NAD(+) reductase


substrate_name: oxidized 2,6-dichlorophenolindolphenol

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADH

proof_lines: mutant R310E, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol

substrate: oxidized 2,6-dichlorophenolindolphenol


proof_lines: mutant R65A, pH 7.5, 25°C, cosubstrate K3Fe(CN)6


substrate_name: NADH

assay_conditions_summary: pH 7.5; 277.15 K; cofactor NADH

proof_lines: mutant K409A, pH 7.5, 25°C, cosubstrate K3Fe(CN)6

substrate: NADH


proof_lines: mutant R310A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol


proof_lines: mutant R339A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol


proof_lines: mutant K387A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol


proof_lines: wild-type, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol


proof_lines: mutant N384A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol


organism: Allochromatium vinosum

substrate_name: NADH; H+; Glutathione disulfide

substrate: NADH; H+; Glutathione disulfide


assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADPH


substrate_name: NADP+

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADP+

substrate: NADP+


substrate_name: Oxidized glutathione

substrate: Oxidized glutathione


substrate_name: H+; NADPH; Glutathione disulfide

substrate: H+; NADPH; Glutathione disulfide


organism: Sus scrofa


organism: Taxus cuspidata


enzyme_display_name: 1,3-beta-oligoglucan phosphorylase

organism: Anaerolinea thermophila

substrate_name: D-glucose

enzyme: 1,3-beta-oligoglucan phosphorylase

substrate: D-glucose


organism: Euglena gracilis


organism: Euglena gracilis var. saccharophila


enzyme_display_name: 4,6-alpha-glucanotransferase (linear substrates/linear products)

organism: Limosilactobacillus reuteri

substrate_name: maltose

substrate: maltose


substrate_name: amylose V

proof_lines:
hydrolysis reaction, pH 5.0, 55°C
transfer reaction, pH 5.0, 55°C

enzyme: 4,6-alpha-glucanotransferase (linear substrates/linear products)

substrate: amylose V


substrate_name: amylose

proof_lines: mutant enzyme I1020M, at pH 5.0 and 40°C

substrate: amylose


proof_lines: mutant enzyme Q1126I, at pH 5.0 and 40°C


proof_lines: mutant enzyme S1057P, at pH 5.0 and 40°C


proof_lines:
wild type enzyme, at pH 5.0 and 40°C
pH 5.0, 40°C, wild-type enzyme


proof_lines: mutant enzyme H1056G, at pH 5.0 and 40°C


enzyme_display_name: reuteransucrase

enzyme: reuteransucrase


enzyme_display_name: [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase

proof_lines: pH 7.5, 30°C, enzyme construct alpha1beta1gamma1

proof_lines: pH 7.5, 30°C, enzyme construct alpha1beta2gamma1

proof_lines: pH 7.5, 30°C, enzyme construct alpha1beta2gamma3


enzyme_display_name: ROCK-subfamily protein kinase

substrate_name: [S6 peptide]-L-serine

proof_lines: purified 66 kDa kinase domain of ROCK-II, at pH 7.4 and 30°C

enzyme: ROCK-subfamily protein kinase

substrate: [S6 peptide]-L-serine


proof_lines: Ki below 0.000005 mM, pH and temperature not specified in the publication

proof_lines: Ki below 0.000001 mM, pH and temperature not specified in the publication


enzyme_display_name: [myosin heavy-chain] kinase

organism: Dictyostelium discoideum

substrate_name: RKKFGESEKTKTKEFL-amide

enzyme: [myosin heavy-chain] kinase

substrate: RKKFGESEKTKTKEFL-amide


proof_lines:
pH 7.0, 25°C
recombinant MHCK B, pH 7.0, 22°C


substrate_name: ATP

assay_conditions_summary: pH 7; 295.15 K; cofactor ATP

substrate: ATP


substrate_name: GRSARVSTYA

assay_conditions_summary: pH 7

proof_lines: in presence of guanosine 5-3-O-(thio)triphosphate-Rac1, pH 7.0

proof_lines: in presence of phosphatidylserine, pH 7.0

substrate: GRSARVSTYA


enzyme_display_name: exoribonuclease H

organism: Human immunodeficiency virus 1

enzyme: exoribonuclease H


enzyme_display_name: deoxyribonuclease IV

organism: Mycobacterium tuberculosis

substrate_name: 5'-end-labeled 29 nucleotide double-stranded DNA

enzyme: deoxyribonuclease IV


organism: Enterococcus faecalis


mutation_type: truncation

proof_lines: truncated enzyme containing an FN3 domain but not C-terminal domains including a CBM32 domain, at pH 6.0 and 50°C


enzyme_display_name: double-stranded uracil-DNA glycosylase

organism: Deinococcus radiodurans

substrate_name: uracil-mismatched double-stranded DNA

proof_lines: T/U containing substrate, wild-type, pH 5.5, 37°C

proof_lines: C/U containing substrate, wild-type, pH 5.5, 37°C

proof_lines: G/hmU containing substrate, wild-type, pH 5.5, 37°C

enzyme: double-stranded uracil-DNA glycosylase

substrate: uracil-mismatched double-stranded DNA


proof_lines: G/U containing substrate, mutant A50E, pH 5.5, 37°C


organism: Mycolicibacterium smegmatis

substrate_name: uracil-containing single stranded DNA

proof_lines: substrate contains A/U base pairs, mutant Q53A/H109A, pH 7.5, 37°C

proof_lines: substrate contains G/U base pairs, mutant Q53A/H109A, pH 7.5, 37°C

substrate: uracil-containing single stranded DNA


proof_lines: substrate contains A/U base pairs, mutant H109A, pH 7.5, 37°C

proof_lines: substrate contains G/U base pairs, mutant H109A, pH 7.5, 37°C


proof_lines: substrate contains G/U base pairs, mutant Q53A, pH 7.5, 37°C

proof_lines: substrate contains A/U base pairs, mutant Q53A, pH 7.5, 37°C


proof_lines: substrate contains A/U base pairs, mutant D56A, pH 7.5, 37°C

proof_lines: substrate contains G/U base pairs, mutant D56A, pH 7.5, 37°C


proof_lines: substrate contains A/U base pairs, mutant E52A, pH 7.5, 37°C

proof_lines: substrate contains G/U base pairs, mutant E52A, pH 7.5, 37°C


proof_lines: substrate contains A/U base pairs, wild-type, pH 7.5, 37°C

proof_lines: substrate contains G/U base pairs, wild-type, pH 7.5, 37°C


enzyme_display_name: site-1 protease

organism: Homo sapiens

proof_lines: pH and temperature not specified in the publication, recombinant mutant Y285A

enzyme: site-1 protease


proof_lines: pH and temperature not specified in the publication, recombinant wild-type enzyme


proof_lines: wild type enzyme, at pH 7.2, temperature not specified in the publication


substrate_name: Abz-VDVADQA-Dap(Dnp)

proof_lines: T7AC-tagged circularly permuted human enzyme, at pH 7.2, temperature not specified in the publication

substrate: Abz-VDVADQA-Dap(Dnp)


enzyme_display_name: (S)-2-methylmalate dehydratase

organism: Escherichia coli

substrate_name: fumarate

enzyme: (S)-2-methylmalate dehydratase

substrate: fumarate


substrate_name: mesaconate

substrate: mesaconate


organism: Paraburkholderia xenovorans


organism: Arabidopsis thaliana


organism: Plasmodium falciparum


organism: Bacillus subtilis

substrate_name: L-Alanine

substrate: L-Alanine


substrate_name: D-Alanine

substrate: D-Alanine


organism: Caldanaerobacter subterraneus subsp. tengcongensis

substrate_name: L-alanine

substrate: L-alanine


substrate_name: D-alanine

substrate: D-alanine


organism: Candidatus Pelagibacter ubique


organism: Clostridioides difficile

proof_lines:
Raw SKiD Env: Temp=-----, pH=8.5
Raw SKiD Env: Temp=37°C, pH=7.5


organism: Geobacillus stearothermophilus


substrate_name: D-Lysine

substrate: D-Lysine


substrate_name: L-lysine

substrate: L-lysine


organism: Mycobacterium avium


organism: Pelagibacter ubique


organism: Pseudomonas aeruginosa

proof_lines: pH and temperature not specified in the publication


substrate_name: D-Ala

substrate: D-Ala


organism: Pseudomonas fluorescens


organism: Pyrococcus horikoshii


organism: Salmonella enteritidis


enzyme_display_name: glutamate racemase

organism: Lactiplantibacillus plantarum

substrate_name: D-glutamate

enzyme: glutamate racemase

substrate: D-glutamate


substrate_name: D-Glutamate

substrate: D-Glutamate


substrate_name: L-glutamate

substrate: L-glutamate


organism: Lactobacillus fermentum

substrate_name: L-Glutamate

substrate: L-Glutamate


organism: Mycobacterium tuberculosis


organism: Bacillus anthracis


assay_conditions_summary: pH 8; 298.15 K; cofactor NAD+

proof_lines: mutant T76A, at 25°C in 50 mM Tris-HCl (pH 8.0), 5 mM NAD+, 0.5 mM iodonitrotetrazolium chloride, 2.5 mM ADP, 20 units of L-glutamate dehydrogenase, 2 units of diaphorase and D-glutamate

proof_lines: mutant T76A, at 25 °C in 50 mM Tris-HCl, pH 8.0, 5 mM NAD+, 0.5 mM iodonitrotetrazolium chloride, 2.5 mM ADP, 20 units of L-glutamate dehydrogenase, 2 units of diaphorase and D-glutamate


substrate_name: Unknown

substrate: Unknown


proof_lines: wild type enzyme, at 25°C in 50 mM Tris-HCl, pH 8.0, 5 mM NAD+, 0.5 mM iodonitrotetrazolium chloride, 2.5 mM ADP, 20 units of L-glutamate dehydrogenase, 2 units of diaphorase and D-glutamate

proof_lines: wild type enzyme, 50 mM boric acid, 100 mM KCl, 0.2 mM dithiothreitol, pH 8.0, at 25°C in the presence of 0.22 microM glutamate racemase

proof_lines: wild type enzyme, 50 mM boric acid, 100 mM KCl, 0.2 mM dithiothreitol, pH 8.0 at 25°C in the presence of 0.22 microM glutamate racemase


assay_conditions_summary: pH 8; buffer potassium phosphate

proof_lines: His-tagged recombinant BsGR mutant V149A, in potassium phosphate buffer (10 mM, pH 8.0)


assay_conditions_summary: pH 8; 310.15 K; buffer potassium phosphate

proof_lines: His-tagged recombinant BsGR, in potassium phosphate buffer (10 mM, pH 8.0)


proof_lines: wild-type enzyme, pH and temperature not specified in the publication


organism: Enterococcus faecalis


organism: Escherichia coli


organism: Fusobacterium nucleatum subsp. nucleatum

proof_lines: His-tagged recombinant FnGR, in potassium phosphate buffer (10 mM, pH 8.0)


organism: Helicobacter pylori


organism: Streptococcus mutans


organism: Streptococcus pyogenes


organism: Thermotoga maritima

assay_conditions_summary: pH 8; 343.15 K; buffer recombinant His-tagged enzyme

proof_lines: recombinant His-tagged enzyme, pH 8.0, 70°C, bicine buffer

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C, bicine buffer


organism: Thermus thermophilus


organism: Wolbachia pipientis

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C


enzyme_display_name: UDP-glucose 4-epimerase

organism: Arabidopsis thaliana

substrate_name: UDPgalactose

enzyme: UDP-glucose 4-epimerase

substrate: UDPgalactose


substrate_name: UDP-D-galactose

assay_conditions_summary: pH 8.6; cofactor NAD+

proof_lines: recombinant enzyme UGE4, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-Glc dehydrogenase

proof_lines: recombinant enzyme UGE4, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase

substrate: UDP-D-galactose


proof_lines: recombinant enzyme UGE5, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase


proof_lines: recombinant Arabidopsis thaliana enzyme UGE1, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-Glc dehydrogenase

proof_lines: recombinant Arabidopsis thaliana enzyme UGE1, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase


proof_lines: recombinant enzyme UGE3, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-Glc dehydrogenase

proof_lines: recombinant enzyme UGE3, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase


proof_lines: recombinant Arabidopsis thaliana enzyme UGE2, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDPGlc dehydrogenase

proof_lines: recombinant Arabidopsis thaliana enzyme UGE2, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase


organism: Aspergillus nidulans

substrate_name: UDP-alpha-D-glucose

substrate: UDP-alpha-D-glucose


substrate_name: UDPglucose

substrate: UDPglucose


organism: Bos taurus


organism: Campylobacter jejuni subsp. jejuni


organism: Davallia divaricata

substrate_name: UDP-alpha-D-galactose

proof_lines: DdUGE1, pH and temperature not specified in the publication

substrate: UDP-alpha-D-galactose


proof_lines: DdUGE2, pH and temperature not specified in the publication


substrate_name: D-tagatose

substrate: D-tagatose


substrate_name: D-galactose

substrate: D-galactose


substrate_name: UDP-galactose

assay_conditions_summary: pH 7.5; 308.15 K; buffer McIlvaine

proof_lines:
in McIlvaine buffer (pH 7.5), at 35°C
Raw SKiD Env: Temp=35°C, pH=7.5

substrate: UDP-galactose


organism: Homo sapiens


organism: Hordeum vulgare

substrate_name: UDP-D-glucose

substrate: UDP-D-glucose


organism: Pyrococcus horikoshii


organism: Streptococcus thermophilus


substrate_name: UDP-N-acetyl-alpha-D-glucosamine

substrate: UDP-N-acetyl-alpha-D-glucosamine


substrate_name: UDP-N-acetyl-alpha-D-galactosamine

substrate: UDP-N-acetyl-alpha-D-galactosamine


proof_lines:
pH 7.0, 80°C
recombinant enzyme, pH 7.0, 80°C
Raw SKiD Env: Temp=80°C, pH=7


enzyme_display_name: actinidain

organism: Unknown

substrate_name: Nalpha-benzoyl-L-Arg p-nitroanilide

enzyme: actinidain

substrate: Nalpha-benzoyl-L-Arg p-nitroanilide


organism: Bacillus anthracis

proof_lines: isozyme RacE2, pH and temperature not specified in the publication

proof_lines: D-glutamate analog, good competitive inhibitor for RacE1

proof_lines: D-glutamate analog, only weak inhibitor of RacE2


organism: Lathyrus oleraceus

substrate_name: UDP-glucose

assay_conditions_summary: pH 8.6; 298.15 K; cofactor NAD+

proof_lines: 50 mM Tris-HCl buffer (pH 8.6), 0.1 mM NAD+, 1 mM UDP-sugar and enzyme

substrate: UDP-glucose


organism: Methanococcus maripaludis


organism: Pisum sativum


substrate_name: UDP-L-arabinose

substrate: UDP-L-arabinose


substrate_name: UDP-D-xylose

substrate: UDP-D-xylose


organism: Pseudomonas aeruginosa


organism: Pyrobaculum calidifontis

proof_lines:
in 100 mM MES-NaOH (pH 5.5), at 70°C
Raw SKiD Env: Temp=70°C, pH=5.5


enzyme_display_name: glutathione-disulfide reductase

organism: Plasmodium falciparum (isolate K1 / Thailand)

substrate_name: NADPH (at 25 degrees Celsius and pH 6.9)

assay_conditions_summary: pH 6.9; cofactor NADPH

proof_lines: kcat is 188.3 sec(-1) with GSSG as substrate (at 25 degrees Celsius and pH 6.9).

enzyme: glutathione-disulfide reductase


organism: Limosilactobacillus reuteri

substrate_name: sucrose

proof_lines: hydrolysis activity, mutant enzyme A1066N/N1134S/N1135E/S1136V, at pH 4.7 and 50°C

proof_lines: total activity, mutant enzyme A1066N/N1134S/N1135E/S1136V, at pH 4.7 and 50°C

proof_lines: transferase activity, mutant enzyme A1066N/N1134S/N1135E/S1136V, at pH 4.7 and 50°C

substrate: sucrose


proof_lines: hydrolysis activity, mutant enzyme P1026V/I1029V/A1066N, at pH 4.7 and 50°C

proof_lines: total activity, mutant enzyme P1026V/I1029V/A1066N, at pH 4.7 and 50°C

proof_lines: transferase activity, mutant enzyme P1026V/I1029V/A1066N, at pH 4.7 and 50°C


proof_lines: transferase activity, mutant enzyme A1066N, at pH 4.7 and 50°C

proof_lines: hydrolysis activity, mutant enzyme A1066N, at pH 4.7 and 50°C

proof_lines: total activity, mutant enzyme A1066N, at pH 4.7 and 50°C


proof_lines: hydrolysis activity, mutant enzyme P1026V/I1029V/A1066N/N1134S/N1135E/S1136V, at pH 4.7 and 50°C

proof_lines: total activity, mutant enzyme P1026V/I1029V/A1066N/N1134S/N1135E/S1136V, at pH 4.7 and 50°C

proof_lines: transferase activity, mutant enzyme P1026V/I1029V/A1066N/N1134S/N1135E/S1136V, at pH 4.7 and 50°C


enzyme_display_name: non-specific serine/threonine protein kinase

organism: Arabidopsis thaliana

substrate_name: Ser/Thr peptide 1 for the protein serine-threonine kinase activity


substrate_name: synthetic substrate

enzyme: non-specific serine/threonine protein kinase

substrate: synthetic substrate


enzyme_display_name: 4-hydroxyphenylpyruvate dioxygenase

substrate_name: 4-hydroxyphenylpyruvic acid

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

enzyme: 4-hydroxyphenylpyruvate dioxygenase

substrate: 4-hydroxyphenylpyruvic acid


organism: Rattus norvegicus


organism: Plasmodium falciparum (isolate 3D7)

substrate_name: glutathione disulfide (GSSG) (at 25 degrees Celsius and pH 6.9)


substrate_name: ATP

assay_conditions_summary: cofactor ATP

substrate: ATP


substrate_name: histone H3 (at pH 7.5 and 30 degrees Celsius)


substrate_name: ATP with or without CaM

substrate: ATP with or without CaM


substrate_name: GLN1-1 (at pH 7.5 and 30 degrees Celsius)


proof_lines: hydrolysis activity, mutant enzyme N1134S/N1135E/S1136V, at pH 4.7 and 50°C

proof_lines: total activity, mutant enzyme N1134S/N1135E/S1136V, at pH 4.7 and 50°C

proof_lines: transferase activity, mutant enzyme N1134S/N1135E/S1136V, at pH 4.7 and 50°C


proof_lines: hydrolysis activity, mutant enzyme P1026V/I1029V, at pH 4.7 and 50°C

proof_lines: total activity, mutant enzyme P1026V/I1029V, at pH 4.7 and 50°C

proof_lines: transferase activity, mutant enzyme P1026V/I1029V, at pH 4.7 and 50°C


proof_lines: total activity, mutant enzyme H1065S/A1066S, at pH 4.7 and 50°C

proof_lines: hydrolysis activity, mutant enzyme H1065S/A1066S, at pH 4.7 and 50°C


proof_lines: hydrolysis activity, mutant enzyme I1029V, at pH 4.7 and 50°C

proof_lines: total activity, mutant enzyme I1029V, at pH 4.7 and 50°C

proof_lines: transferase activity, mutant enzyme I1029V, at pH 4.7 and 50°C


organism: Enterococcus faecalis


proof_lines: hydrolysis activity, wild type enzyme, at pH 4.7 and 50°C

proof_lines: total activity, wild type enzyme, at pH 4.7 and 50°C

proof_lines: transferase activity, wild type enzyme, at pH 4.7 and 50°C


proof_lines: total activity, mutant enzyme P1026V, at pH 4.7 and 50°C

proof_lines: transferase activity, mutant enzyme P1026V, at pH 4.7 and 50°C

proof_lines: hydrolysis activity, mutant enzyme P1026V, at pH 4.7 and 50°C


proof_lines: hydrolysis activity, mutant enzyme P1026V/I1029V/N1134S/N1135E/S1136V, at pH 4.7 and 50°C

proof_lines: total activity, mutant enzyme P1026V/I1029V/N1134S/N1135E/S1136V, at pH 4.7 and 50°C

proof_lines: transferase activity, mutant enzyme P1026V/I1029V/N1134S/N1135E/S1136V, at pH 4.7 and 50°C


organism: Homo sapiens

substrate_name: UDP-galactose


proof_lines: wild type enzyme, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 37°C

proof_lines: wild type enzyme, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 24°C


substrate_name: D-glutamate

assay_conditions_summary: pH 9.2; 298.15 K; cofactor NAD+

proof_lines: mutant Y221A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0

proof_lines: mutant Y221A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol

substrate: D-glutamate


substrate_name: L-glutamate

assay_conditions_summary: pH 8.2; 298.15 K; cofactor NAD+

proof_lines: mutant R25A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol

proof_lines: mutant R25A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.

substrate: L-glutamate


proof_lines: mutant R214A/K106A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase,

proof_lines: mutant R214A/K106A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol


proof_lines: mutant K29A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol

proof_lines: mutant K29A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.

proof_lines: mutant K29A, in 10 mM potassium phosphate (pH 8.2)and 0.2 mM dithiothreitol


enzyme_display_name: alkane 1-monooxygenase

organism: Oryctolagus cuniculus

substrate_name: laurate

proof_lines:
CYP4A4wt, omega-hydroxylation
CYP4A7wt, omega-hydroxylation

enzyme: alkane 1-monooxygenase

substrate: laurate


proof_lines: CYP4A7, W93S mutant, omega-hydroxylation


substrate_name: prostaglandin E1

proof_lines: CYP4A7, S255F mutant, omega-hydroxylation

substrate: prostaglandin E1


substrate_name: arachidonic acid

proof_lines: CYP4A7, H206Y mutant, omega-hydroxylation

substrate: arachidonic acid


proof_lines: CYP4A7, R90W mutant, omega-hydroxylation


proof_lines: CYP4A7, R90W/W93S mutant, omega-hydroxylation


proof_lines: CYP4A7, H206Y/S255F mutant, omega-hydroxylation


organism: Limosilactobacillus fermentum


organism: Marinithermus hydrothermalis

substrate_name: UDP-N-acetyl-alpha-D-glucosamine


proof_lines: pH 8, 30°C, mutant enzyme D36N and H152Q


organism: Aquifex pyrophilus


substrate_name: L-Glutamate

substrate: L-Glutamate


proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=-----, pH=8.5


organism: Bacillus anthracis

substrate_name: D-Glutamate

substrate: D-Glutamate


organism: Phocaeicola plebeius

proof_lines: truncated mutant enzyme D465S, at pH 7.0 and 37°C


proof_lines: truncated mutant enzyme D465A, at pH 7.0 and 37°C


proof_lines: truncated mutant enzyme D465G, at pH 7.0 and 37°C


proof_lines: wild type full-length enzyme, at pH 7.0 and 37°C

proof_lines: wild type truncated enzyme, at pH 7.0 and 37°C


organism: Helicobacter pylori


substrate_name: Unknown

proof_lines: value above 100 for deletion mutant E151K

substrate: Unknown


proof_lines: demonstrates a high degree of asymmetry in substrate processing with the Michaelis constant for D-glutamate approximately tenfold lower than for L-glutamate


substrate_name: lauric acid

substrate: lauric acid


organism: Escherichia coli

substrate_name: 21-mer C Grec

substrate: 21-mer C Grec


proof_lines: mutant H69A, pH 7.6, 37°C, in presence of Zn2+


organism: Saccoglossus kowalevskii

substrate_name: D-Aspartate

assay_conditions_summary: pH 8

proof_lines: mutant enzyme A156S, pH 8.0, temperature not specified in the publication, Asp/GluR enzyme

proof_lines: pH 8.0, temperature not specified in the publication, mutant enzyme A156S, Asp/GluR enzyme

substrate: D-Aspartate


proof_lines: mutant enzyme S156A, pH 8.0, temperature not specified in the publication, AspR enzyme


proof_lines: pH 8.0, temperature not specified in the publication, wild-type enzyme, Asp/GluR enzyme

proof_lines: wild-type enzyme, pH 8.0, temperature not specified in the publication, Asp/GluR enzyme

proof_lines: wild-type enzyme, pH 8.0, temperature not specified in the publication, AspR enzyme


organism: Aeromonas hydrophila

assay_conditions_summary: pH 8; 298.15 K; buffer 20 mM sodium phosphate

proof_lines: mutant enzyme Y149G, in 20 mM sodium phosphate buffer, pH 8.0, at 25°C

substrate: UDP-galactose


proof_lines: wild type recombinant enzyme, in 20 mM sodium phosphate buffer, pH 8.0, at 25°C


proof_lines: mutant enzyme K153N, in 20 mM sodium phosphate buffer, pH 8.0, at 25°C


enzyme_display_name: biphenyl 2,3-dioxygenase

organism: Paraburkholderia xenovorans

substrate_name: biphenyl

proof_lines: wild type enzyme, at 37°C, pH not specified in the publication

enzyme: biphenyl 2,3-dioxygenase

substrate: biphenyl


proof_lines: mutant enzyme S283M, at 37°C, pH not specified in the publication


substrate_name: UDP-alpha-D-glucose

proof_lines: pH 6.2, temperature not specified in the publication, recombinant wild-type enzyme

substrate: UDP-alpha-D-glucose


proof_lines: pH 6.2, temperature not specified in the publication, recombinant mutant Y149F


proof_lines: pH 6.2, temperature not specified in the publication, recombinant mutant K153M


organism: Acanthamoeba castellanii

substrate_name: GRGRSSVYS

assay_conditions_summary: pH 7; 303.15 K; cofactor ATP

proof_lines: recombinant wild-type catalytic domain, pH 7.0, 30°C

substrate: GRGRSSVYS


proof_lines: recombinant T632A mutant catalytic domain, pH 7.0, 30°C


organism: Pseudomonas sp.

assay_conditions_summary: 298.15 K; cofactor NADH


substrate_name: UDPgalactose

proof_lines: pH 6.2, temperature not specified in the publication, recombinant S124T

substrate: UDPgalactose


proof_lines: pH 6.2, temperature not specified in the publication, recombinant mutant S124A


substrate_name: DNA-RNA hybrid

substrate: DNA-RNA hybrid


organism: Comamonas testosteroni

proof_lines:
parental protein
recombinant, purified protein
recombinant His-tagged protein


organism: Ectopseudomonas oleovorans

proof_lines: Pseudomonas pseudoalcaligenes strain LB400

proof_lines: Pseudomonas pseudoalcaligenes strain 1072

proof_lines: Pseudomonas pseudoalcaligenes strain KF707


organism: Pandoraea pnomenusa


organism: Pusillimonas sp. T7-7

substrate_name: pentadecane

proof_lines: pH 7.5, 30°C, recombinant alkane hydroxylase large subunit

substrate: pentadecane


organism: Fusarium oxysporum


organism: Homo sapiens

substrate_name: palmitate

proof_lines:
omega-hydroxylation
(omega-1)-hydroxylation
gene CYP4A11

substrate: palmitate


organism: Pseudomonas putida

proof_lines: inhibits oxidation of methyl tert-butyl ether


enzyme_display_name: assimilatory sulfite reductase (NADPH)

substrate_name: sulfite

assay_conditions_summary: cofactor NADPH

proof_lines: cosubstrate 3-acetylpyridineadenine dinucleotide phosphate

enzyme: assimilatory sulfite reductase (NADPH)

substrate: sulfite


organism: Methanocaldococcus jannaschii

substrate_name: reduced F420

proof_lines: at a fixed sulfite concentration of 0.29 mM and within a reduced F420 concentration range of 0.004-0.06 mM

proof_lines: within a sulfite concentration range of 0.0014-0.3 mM and with a fixed reduced F420 concentration of 0.04 mM

substrate: reduced F420


organism: Saccharomyces bayanus


organism: Saccharomyces cerevisiae

substrate_name: NADPH

proof_lines:
reduction of cytochrome c
reduction of sulfite

substrate: NADPH


organism: Oryctolagus cuniculus

substrate_name: GSSG

substrate: GSSG


organism: Pteris vittata

assay_conditions_summary: pH 7; buffer 50 mM potassium phosphate; cofactor NADPH

proof_lines: in 50 mM potassium phosphate buffer (pH 7.0)


organism: Setaria cervi

assay_conditions_summary: pH 7; 295.15 K; buffer pH 7

proof_lines: in 50 mM potassium phosphate buffer pH 7.0, at 37°C


organism: Allochromatium vinosum

substrate_name: NADH

assay_conditions_summary: cofactor NADH

substrate: NADH


organism: Equus caballus

substrate_name: ferricyanide

substrate: ferricyanide


organism: Escherichia coli

substrate_name: glutathione disulfide

assay_conditions_summary: pH 7.5; cofactor NADPH

proof_lines: pH 7.5, temperature not specified in the publication

substrate: glutathione disulfide


enzyme_display_name: 1,3-beta-oligoglucan phosphorylase

organism: Euglena gracilis

substrate_name: phosphate

proof_lines:
pH 7.2, with laminaribiose
+ laminaribiose
pH 7.2, with laminaritetraose

enzyme: 1,3-beta-oligoglucan phosphorylase

substrate: phosphate


substrate_name: alpha-D-glucosamine 1-phosphate

proof_lines: with cellobiose as acceptor, at pH 7.0 and 37°C

proof_lines: with laminatriose as acceptor, at pH 7.0 and 37°C

substrate: alpha-D-glucosamine 1-phosphate


enzyme_display_name: 4,6-alpha-glucanotransferase (linear substrates/linear products)

organism: Limosilactobacillus reuteri

substrate_name: maltose

substrate: maltose


enzyme_display_name: reuteransucrase

substrate_name: sucrose

proof_lines: His6-tagged enzyme, with maltose as cosubstrate, total activity, at pH 4.7 and 50°C

proof_lines: His6-tagged enzyme, tranferase activity, at pH 4.7 and 50°C

proof_lines: His6-tagged enzyme, total activity, at pH 4.7 and 50°C

enzyme: reuteransucrase

substrate: sucrose


organism: Streptococcus thermophilus

substrate_name: amylose

substrate: amylose


enzyme_display_name: [myosin heavy-chain] kinase

organism: Acanthamoeba castellanii

substrate_name: GRGRSSVYS

proof_lines: phospholipid-bound, unphosphorylated enzyme, pH 7.0, 30°C

enzyme: [myosin heavy-chain] kinase

substrate: GRGRSSVYS


organism: Dictyostelium discoideum

substrate_name: RKKFGESEKTKTKEFL-amide

substrate: RKKFGESEKTKTKEFL-amide


enzyme_display_name: [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase

organism: Brassica oleracea

substrate_name: HMKSAMSGLHLVKRR

assay_conditions_summary: pH 7; 303.15 K; cofactor CoA

proof_lines:
pH 7.0, 30°C, kinase B
pH 7.0, 30°C, kinase A

substrate: HMKSAMSGLHLVKRR


organism: Rattus norvegicus

substrate_name: ATP

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

proof_lines:
pH 7.5, 37°C
pH 6.5, 30°C, low-MW kinase
in absence of AMP

enzyme: [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase

substrate: ATP


enzyme_display_name: exoribonuclease H

organism: Human immunodeficiency virus 1

substrate_name: poly(rA)n-poly(dT)n

proof_lines:
mutant p66C280P/p51C280P
mutant p66C280W/p51
mutant p66/p51C280P

enzyme: exoribonuclease H

substrate: poly(rA)n-poly(dT)n


substrate_name: DNA-16-nucleotide-RNA hybrid

proof_lines: pH 7.8, 37°C, recombinant wild-type enzyme

substrate: DNA-16-nucleotide-RNA hybrid


substrate_name: 18mer DNA-RNA hybrid

substrate: 18mer DNA-RNA hybrid


enzyme_display_name: venom exonuclease

organism: Walterinnesia aegyptia

substrate_name: Unknown

enzyme: venom exonuclease

substrate: Unknown


organism: Bothrops alternatus

substrate_name: bis(p-nitrophenyl)-phosphate

substrate: bis(p-nitrophenyl)-phosphate


organism: Bothrops atrox


organism: Bothrops jararacussu

substrate_name: bis(4-nitrophenyl) phosphate

substrate: bis(4-nitrophenyl) phosphate


organism: Crotalus adamanteus

substrate_name: uridine oligonucleotides

proof_lines: with different degrees of polymerization

proof_lines: depending on nucleobase and presence of Mg2+

substrate: uridine oligonucleotides


organism: Crotalus durissus terrificus


organism: Daboia siamensis

substrate_name: bis-p-nitrophenyl phosphate

substrate: bis-p-nitrophenyl phosphate


organism: Trimeresurus stejnegeri

substrate_name: nicotinamide adenine dinucleotide

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

substrate: nicotinamide adenine dinucleotide


organism: Vipera aspis

substrate_name: thymidine 5'-p-nitrophenyl phosphate

substrate: thymidine 5'-p-nitrophenyl phosphate


enzyme_display_name: deoxyribonuclease IV

organism: Thermococcus eurythermalis

substrate_name: ssDNA containing a (polyethylene glycol)3 spacer

enzyme: deoxyribonuclease IV


enzyme_display_name: double-stranded uracil-DNA glycosylase

organism: Escherichia coli

enzyme: double-stranded uracil-DNA glycosylase


enzyme_display_name: site-1 protease

organism: Homo sapiens

substrate_name: Ac-Arg-Ser-Leu-Lys-p-nitroanilide

proof_lines: in 25 mM Tris, 25 mM MES, 2 mM CaCl2, pH 7.4, at 37°C

enzyme: site-1 protease

substrate: Ac-Arg-Ser-Leu-Lys-p-nitroanilide


organism: Mammalia


organism: Bacteroides caccae

proof_lines: wild type truncated enzyme, at pH 7.0 and 37°C

proof_lines: wild type full-length enzyme, at pH 7.0 and 37°C


organism: Bombyx mori


enzyme_display_name: human endogenous retrovirus K endopeptidase

proof_lines: pH 5.0, 25°C, 13000 Da C-terminally truncated enzyme form


proof_lines:
pH 4.5, 18200 Da enzyme form
pH 4.5, 11600 Da core domain
pH 5.0, 25°C, 18000 Da enzyme form

enzyme: human endogenous retrovirus K endopeptidase


substrate_name: acetyl-VDVADGW-amide

substrate: acetyl-VDVADGW-amide


enzyme_display_name: actinidain

organism: Actinidia chinensis

substrate_name: benzyloxycarbonyl 4-nitrophenyl ester

proof_lines: above 0.25 mM, at 4.3% acetonitrile, 25°C, pH 5.3, parameter for pre-steady-state acylation phase

enzyme: actinidain

substrate: benzyloxycarbonyl 4-nitrophenyl ester


organism: Actinidia deliciosa

substrate_name: casein

proof_lines: 60°C, pH not specified in the publication

substrate: casein


enzyme_display_name: neutrophil collagenase

substrate_name: bovine collagen I

proof_lines: pH 7.0, 37°C, unprotonated and 1-protonated substrate

enzyme: neutrophil collagenase

substrate: bovine collagen I


enzyme_display_name: (S)-2-methylmalate dehydratase

organism: Aerobic bacterium

substrate_name: mesaconate

enzyme: (S)-2-methylmalate dehydratase

substrate: mesaconate


organism: Clostridium tetanomorphum

substrate_name: L-citramalate

substrate: L-citramalate


substrate_name: (S)-malate

substrate: (S)-malate


enzyme_display_name: glutamate racemase

organism: Enterococcus faecalis

substrate_name: D-glutamate

proof_lines: pH and temperature not specified in the publication

enzyme: glutamate racemase

substrate: D-glutamate


substrate_name: L-glutamate

proof_lines: in presence of 0.0065 mM of the activator UDP-N-acetylmuramyl-L-Ala

substrate: L-glutamate


organism: Fusobacterium nucleatum

substrate_name: D-homocysteic acid

substrate: D-homocysteic acid


organism: Lactiplantibacillus plantarum

substrate_name: D-Glu

substrate: D-Glu


enzyme_display_name: UDP-glucose 4-epimerase

substrate_name: UDP-glucose

proof_lines: fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker

enzyme: UDP-glucose 4-epimerase

substrate: UDP-glucose


organism: Galdieria sulphuraria

substrate_name: UDPgalactose

substrate: UDPgalactose


substrate_name: UDP-galactose

proof_lines: mutant enzyme M284K, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 24°C

proof_lines: mutant enzyme M284K, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 37°C

substrate: UDP-galactose


organism: Hordeum vulgare

substrate_name: UDP-Gal

substrate: UDP-Gal


organism: Levilactobacillus brevis


organism: Limosilactobacillus fermentum


organism: Mycobacterium tuberculosis

substrate_name: Unknown

substrate: Unknown


organism: Pediococcus pentosaceus

substrate_name: L-Glu

substrate: L-Glu


organism: Aeromonas hydrophila


organism: Arabidopsis thaliana

proof_lines: The approximate value is estimated from reaction velocities at saturating substrate concentrations (5–9 mM).

proof_lines: Different enzyme concentrations give slight variations in activity.

proof_lines: values are indirectly determined from the Haldane relationship


organism: Bifidobacterium bifidum

substrate_name: UDPglucose

substrate: UDPglucose


organism: Campylobacter jejuni

substrate_name: UDP-GlcNAc

substrate: UDP-GlcNAc


organism: Thermus thermophilus

assay_conditions_summary: pH 8.9; 323.15 K; cofactor NAD+

proof_lines: recombinant enzyme, 100 mM glycine-NaOH (pH 8.9), 4 mM UDP-galactose, 1 mM beta-NAD+, 8.3 mM MgCl2, and 5.4 U of UDP-glucose dehydrogenase


organism: Torulopsis candida


organism: Vicia faba


organism: Kluyveromyces marxianus

proof_lines:
native and renatured enzyme
25°C, pH 8.8


organism: Magallana gigas

substrate_name: UDP-alpha-D-galactose

substrate: UDP-alpha-D-galactose


organism: Poterioochromonas malhamensis


organism: Saccharomyces cerevisiae


enzyme_display_name: glutathione-disulfide reductase

organism: Bos taurus

substrate_name: H+; NADPH; Glutathione disulfide

assay_conditions_summary: pH 7; 303.15 K; cofactor NADPH

proof_lines:
calf liver enzyme, pH 7.0, 30°C
pH 7.4, 37°C
erythrocyte enzyme, pH 7.0, 30°C

enzyme: glutathione-disulfide reductase

substrate: H+; NADPH; Glutathione disulfide


organism: Euglena gracilis

substrate_name: H+; NADPH; Oxidized glutathione

substrate: H+; NADPH; Oxidized glutathione


organism: Enterococcus faecium

substrate_name: L-Glutamate

substrate: L-Glutamate


organism: Staphylococcus aureus


enzyme_display_name: alanine racemase

organism: Enterococcus faecalis

substrate_name: D-Alanine

enzyme: alanine racemase

substrate: D-Alanine


organism: Streptomyces lavendulae


proof_lines:
liver enzyme
mammary enzyme


organism: Pinus sylvestris

substrate_name: UDP-D-galactose

substrate: UDP-D-galactose


organism: Populus canadensis


enzyme_display_name: S-ribosylhomocysteine lyase

organism: Escherichia coli

substrate_name: S-Ribosylhomocysteine

enzyme: S-ribosylhomocysteine lyase

substrate: S-Ribosylhomocysteine


substrate_name: S-ribosylhomocysteine

proof_lines:
Co2+ substitued enzyme
Co2+ substituted enzyme

substrate: S-ribosylhomocysteine


substrate_name: S-Ribosyl-L-homocysteine

substrate: S-Ribosyl-L-homocysteine


organism: Streptococcus suis


enzyme_display_name: progesterone 11alpha-monooxygenase

organism: Priestia megaterium

substrate_name: progesterone

proof_lines: pH not specified in the publication, 30°C, mutant A243V/A395W/G397K

enzyme: progesterone 11alpha-monooxygenase

substrate: progesterone


proof_lines: pH not specified in the publication, 30°C, mutant A395W/G397K


proof_lines: pH not specified in the publication, 30°C, mutant T248V/A395I


proof_lines: pH not specified in the publication, 30°C, mutant A106T/A395W/G379K/R409L


proof_lines: pH not specified in the publication, 30°C, mutant M155I/F165L/A395I


organism: Pseudomonas savastanoi pv. phaseolicola

smiles: O=C(O)CCCC(=O)C(=O)O


substrate_name: L-arginine

proof_lines: with 2-oxoadipate as cosubstrate, at pH 7.5 and 25°C

substrate: L-arginine


substrate_name: L-Arginine

substrate: L-Arginine


organism: Pseudomonas syringae


organism: Homo sapiens

substrate_name: uracil in ASH1 E3 mRNA

substrate: uracil in ASH1 E3 mRNA


enzyme_display_name: retroviral ribonuclease H

organism: Human immunodeficiency virus 1

substrate_name: Unknown

proof_lines: presence of Mg2+ and DNA:RNA hybrid, pH 8.0

enzyme: retroviral ribonuclease H

substrate: Unknown


enzyme_display_name: chymase

substrate_name: Angiotensin I; H2O

enzyme: chymase

substrate: Angiotensin I; H2O


enzyme_display_name: gelatinase B

proof_lines:
pH 7.5, 37°C, monomeric form
pH 7.5, 37°C, dimeric form

enzyme: gelatinase B


organism: Bacillus subtilis


proof_lines:
Fe2+ containing BsLuxS
Zn2+ substituted BsLuxS
Co2+ substituted BsLuxS


proof_lines: pH not specified in the publication, 30°C, mutant A395I


proof_lines: pH not specified in the publication, 30°C, mutant T247W/A395W/G379K


proof_lines: pH not specified in the publication, 30°C, mutants A395II


proof_lines: pH not specified in the publication, 30°C, mutant T89N/A395I


proof_lines: pH not specified in the publication, 30°C, mutant T247V/A395I


proof_lines: pH not specified in the publication, 30°C, mutant A243V/A395I


proof_lines: pH not specified in the publication, 30°C, wild-type


proof_lines: pH not specified in the publication, 30°C, mutant A106T/A395I


proof_lines: pH not specified in the publication, 30°C, mutants A395I and M155I/F165L/A395I


proof_lines: pH not specified in the publication, 30°C, mutant D217V/A395I


proof_lines: pH not specified in the publication, 30°C, mutant A106T/A395I/R409L


proof_lines: pH not specified in the publication, 30°C, mutant T89N/A106T/A395I/R409L


enzyme_display_name: ribose-5-phosphate isomerase

organism: Acetivibrio thermocellus

substrate_name: D-Psicose

enzyme: ribose-5-phosphate isomerase

substrate: D-Psicose


substrate_name: D-ribose

proof_lines:
pH 7.5, 65°C
Raw SKiD Env: Temp=65°C, pH=7.5
Raw SKiD Env: Temp=80°C, pH=7.5

substrate: D-ribose


organism: Brucella anthropi

substrate_name: L-rhamnose

substrate: L-rhamnose


substrate_name: D-allose

substrate: D-allose


proof_lines: recombinant mutant S9T/S98H/S134H, pH 7.5, 50Â°C


proof_lines: recombinant mutant S98H/S134H, pH 7.5, 50Â°C


proof_lines: pH 7.0, 45°C, recombinant wild-type enzyme

proof_lines: recombinant wild-type enzyme, pH 7.5, 50Â°C


proof_lines: pH 7.5, 45°C, recombinant mutant A10T/T32S/G101N


proof_lines: pH 7.5, 45°C, recombinant wild-type enzyme


substrate_name: D-psicose

substrate: D-psicose


organism: Clostridioides difficile

substrate_name: L-talose

substrate: L-talose


substrate_name: D-Ribose 5-phosphate

substrate: D-Ribose 5-phosphate


substrate_name: D-ribose 5-phosphate

substrate: D-ribose 5-phosphate


organism: Mycobacterium tuberculosis


substrate_name: D-Allose 6-phosphate

substrate: D-Allose 6-phosphate


proof_lines: pH 7.5, 37°C, RpiB, substrate is D-ribose 5-phosphate


organism: Pyrococcus horikoshii


substrate_name: D-ribulose 5-phosphate

proof_lines:
50°C, pH 6.0, wild-type enzyme
93°C, pH 6, wild-type enzyme

substrate: D-ribulose 5-phosphate


proof_lines:
Raw SKiD Env: Temp=50°C, pH=6
Raw SKiD Env: Temp=93°C, pH=6


organism: Saccharomyces cerevisiae


organism: Klebsiella aerogenes


organism: Leishmania donovani


organism: Spinacia oleracea


organism: Thermotoga maritima


enzyme_display_name: dactylysin

organism: Unknown

substrate_name: Substance P

enzyme: dactylysin

substrate: Substance P


substrate_name: S-ribosylhomocysteine

substrate: S-ribosylhomocysteine


organism: Vibrio harveyi


proof_lines:
pH 8.0 and 70°C
pH 8.0, 70°C
Raw SKiD Env: Temp=70°C, pH=8


organism: Trypanosoma cruzi

substrate_name: D-Ribose 5-diphosphate

substrate: D-Ribose 5-diphosphate


proof_lines: in 50 mM Tris-HCl (pH 7.6), 150 mM NaCl and 5 mM MESNA, at 30°C


substrate_name: RNA-DNA hybrid

substrate: RNA-DNA hybrid


enzyme_display_name: [beta-adrenergic-receptor] kinase

organism: Bos taurus

substrate_name: ATP

assay_conditions_summary: pH 7.8; 296.15 K; cofactor ATP

proof_lines: pH 7.8, 23°C, recombinant wild-type GRK2, with substrate tubulin

enzyme: [beta-adrenergic-receptor] kinase

substrate: ATP


proof_lines: pH 7.8, 23°C, recombinant GRK2 C221V mutant, with substrate tubulin


proof_lines: pH 7.8, 23°C, recombinant GRK2 L271G mutant, with substrate tubulin


proof_lines:
native enzyme
pH 7.0, 22°C


enzyme_display_name: hippurate hydrolase

organism: Campylobacter jejuni

substrate_name: hippurate

enzyme: hippurate hydrolase

substrate: hippurate


substrate_name: N-acetylglycine

proof_lines: pH 7.5, 37°C, purified recombinant wild-type enzyme

substrate: N-acetylglycine


proof_lines: pH 7.5, 37°C, native enzyme in cell extract


organism: Escherichia coli

substrate_name: uracil54 in unmodified E. coli tRNA1-Val


enzyme_display_name: lysine/arginine leucyltransferase

substrate_name: KQC-acrydonylalanine

proof_lines: amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, 4-bromo phenylalanine, Kcat apparent

enzyme: lysine/arginine leucyltransferase

substrate: KQC-acrydonylalanine


substrate_name: beta-adrenergic receptor

proof_lines: pH 7.5, 30°C, recombinant beta-ARK 1, rhodopsin as substrate

substrate: beta-adrenergic receptor


organism: Homo sapiens

substrate_name: GEGMDEMEFTEAESNMN

proof_lines: Km above 0.1 mM, pH and temperature not specified in the publication

substrate: GEGMDEMEFTEAESNMN


enzyme_display_name: ADP ribose phosphorylase

organism: Euglena gracilis

substrate_name: ADP-ribose

assay_conditions_summary: pH 7.8; 308.15 K; cofactor ADP

proof_lines:
pH 7.8, 35°C
pH 7.8, 30°C
ADP/phosphate exchange

enzyme: ADP ribose phosphorylase

substrate: ADP-ribose


organism: Human immunodeficiency virus 1

substrate_name: poly(rA)n-poly(dT)n

proof_lines:
37°C, mutant p66C280P/p51
37°C, mutant p66/p51C280P
37°C, mutant p66/p51C280W

substrate: poly(rA)n-poly(dT)n


substrate_name: Unknown

substrate: Unknown


substrate_name: vitronectin of MW 65000

proof_lines:
75000 MW form
pH 7.6, 25°C, recombinant enzyme

substrate: vitronectin of MW 65000


organism: Mus musculus

substrate_name: SGQPHYLTTA

proof_lines:
pH 7.5, 37°C, peptide B1
pH 7.5, 37°C, peptide A11m2
pH 7.5, 37°C, peptide A10

substrate: SGQPHYLTTA


enzyme_display_name: mitochondrial intermediate peptidase

proof_lines: in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25°C

enzyme: mitochondrial intermediate peptidase


substrate_name: atrial natriuretic peptide

substrate: atrial natriuretic peptide


organism: Xenopus laevis

substrate_name: dynorphin A

proof_lines:
[Leu5,Arg6]enkephalin
(6-11)substance P hexapeptide
70% inhibition when tested on kermit

substrate: dynorphin A


organism: Fusarium incarnatum


organism: Pseudomonas putida

substrate_name: N-benzoyl-L-alanine

substrate: N-benzoyl-L-alanine


organism: Acetivibrio thermocellus

substrate_name: L-ribulose

assay_conditions_summary: pH 7.5; 338.15 K; buffer 50 mM Tris-HCl

proof_lines: in 50 mM Tris-HCl buffer (pH 7.5), at 65°C

substrate: L-ribulose


substrate_name: D-ribose 5-phosphate

proof_lines:
calf spleen
ox muscle
calf liver

substrate: D-ribose 5-phosphate


organism: Clostridioides difficile

substrate_name: D-ribose

substrate: D-ribose


organism: Cyberlindnera jadinii

proof_lines:
enzyme form III
enzyme form II
enzyme form I


organism: Echinococcus granulosus


proof_lines: pH 7.5, 37°C, RpiB, substrate is D-ribose 5-phosphate


organism: Klebsiella aerogenes


organism: Bacillus subtilis


organism: Lactiplantibacillus plantarum

substrate_name: S-ribosylhomocysteine

proof_lines:
isoform LuxS1, pH 8.0, 45°C
isoform LuxS2, pH 8.0, 37°C

substrate: S-ribosylhomocysteine


organism: Thermus thermophilus


organism: Lathyrus oleraceus

substrate_name: D-ribulose 5-phosphate

substrate: D-ribulose 5-phosphate


organism: Mycobacterium tuberculosis


organism: Pediococcus pentosaceus


organism: Pseudothermotoga lettingae

substrate_name: D-psicose

substrate: D-psicose


organism: Rhodospirillum rubrum


organism: Saccharomyces cerevisiae


organism: Spinacia oleracea


organism: Thermotoga maritima

proof_lines:
at 60°C and pH 7.0
at 80°C and pH 7.0


organism: Vibrio harveyi

substrate_name: S-Ribosylhomocysteine

substrate: S-Ribosylhomocysteine


organism: Salmonella typhimurium

substrate_name: L-Alanine

substrate: L-Alanine


substrate_name: L-alanine

substrate: L-alanine


organism: Treponema denticola


substrate_name: D-Alanine

substrate: D-Alanine


substrate_name: L-Alanine methyl ester

substrate: L-Alanine methyl ester


organism: Wolbachia pipientis

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C


organism: Aquifex pyrophilus

substrate_name: L-glutamate

substrate: L-glutamate


organism: Acetivibrio thermocellus

substrate_name: D-Psicose

substrate: D-Psicose


organism: Streptococcus suis


organism: Vibrio cholerae

substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication

substrate: Unknown


proof_lines:
Co2+ substituted VhLuxS
Co2+ substituted enzyme


organism: Arabidopsis thaliana


organism: Escherichia coli


organism: Mycobacterium tuberculosis


organism: Plasmodium falciparum

proof_lines:
pH 7.0, 27°C
Raw SKiD Env: Temp=27°C, pH=7


organism: Salmonella enteritidis


organism: Aquifex aeolicus

substrate_name: S-adenosyl-L-methionine

proof_lines: wild-type, 55°C, pH not specified in the publication

substrate: S-adenosyl-L-methionine


proof_lines: mutant Y167F, 55°C, pH not specified in the publication


proof_lines: mutant T132S, 55°C, pH not specified in the publication


proof_lines: mutant E168A, 55°C, pH not specified in the publication