column_label: Table 2, row Ceftriaxone: column "k cat (s^-1)" cell = 620; column "K m (uM)" cell = 28; column "k cat / K m (mM^-1 s^-1)" cell = 22 150. k cat and K m are read from the same single printed table row, so the tuple is one measurement and spans no other row, column or cofactor block.

normalized_value_unit_evidence: k cat 620 s^-1 normalizes to 620.0 s^-1. K m 28 uM normalizes to 28/1000 = 0.028 mM. k cat / K m = 620.0 s^-1 / 0.028 mM = 22142.85714285714 s^-1 mM^-1, the quotient of the two printed cells of this same row. Table 2 also prints a rounded k cat / K m cell for this row, 22 150 mM^-1 s^-1; the exported ratio is the full-precision quotient 620 / (28 uM as mM) of the same row's two printed cells, which rounds to that printed cell.

raw_value_unit_evidence: Table 2 (PDF page 5 / article page 62), row Ceftriaxone: k cat cell 620 s^-1; K m cell 28 uM; k cat / K m cell 22 150 mM^-1 s^-1. Verbatim line: Ceftriaxone 620 28 22 150

row_label: Ceftriaxone


enzyme_display_name: beta-lactamase

organism: Serratia fonticola

substrate_name: Cloxacillin

source_db: oed

verification_status: verified

ki_display: Not reported

mutation_signature: Unknown

protein_accession_database: UniProt

kcat_over_km_origin: calculated

source_license: CC BY 4.0

km_unit: mM

sequence_source: UniProtKB reviewed accession P80545 (BLAC_SERFO), direct REST lookup on 2026-05-27; PMID 9300809 is the UniProt protein-sequence reference.

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

column_label: Table 2, row Cloxacillin: column "k cat (s^-1)" cell = 90; column "K m (uM)" cell = 43; column "k cat / K m (mM^-1 s^-1)" cell = 2 100. k cat and K m are read from the same single printed table row, so the tuple is one measurement and spans no other row, column or cofactor block.

normalized_value_unit_evidence: k cat 90 s^-1 normalizes to 90.0 s^-1. K m 43 uM normalizes to 43/1000 = 0.043 mM. k cat / K m = 90.0 s^-1 / 0.043 mM = 2093.0232558139537 s^-1 mM^-1, the quotient of the two printed cells of this same row. Table 2 also prints a rounded k cat / K m cell for this row, 2 100 mM^-1 s^-1; the exported ratio is the full-precision quotient 90 / (43 uM as mM) of the same row's two printed cells, which rounds to that printed cell.

raw_value_unit_evidence: Table 2 (PDF page 5 / article page 62), row Cloxacillin: k cat cell 90 s^-1; K m cell 43 uM; k cat / K m cell 2 100 mM^-1 s^-1. Verbatim line: Cloxacillin 90 43 2 100

row_label: Cloxacillin

table_label: Table 2. Kinetic parameters of b-lactamase of Serratia fonticola CUV

data_origin: experimental

enzyme: beta-lactamase

substrate: Cloxacillin

status: verified

source: oed


substrate_name: Cefoperazone

column_label: Table 2, row Cefoperazone: column "k cat (s^-1)" cell = 70; column "K m (uM)" cell = 10; column "k cat / K m (mM^-1 s^-1)" cell = 7 000. k cat and K m are read from the same single printed table row, so the tuple is one measurement and spans no other row, column or cofactor block.

normalized_value_unit_evidence: k cat 70 s^-1 normalizes to 70.0 s^-1. K m 10 uM normalizes to 10/1000 = 0.01 mM. k cat / K m = 70.0 s^-1 / 0.01 mM = 7000.0 s^-1 mM^-1, the quotient of the two printed cells of this same row. Table 2 also prints a rounded k cat / K m cell for this row, 7 000 mM^-1 s^-1; the exported ratio is the full-precision quotient 70 / (10 uM as mM) of the same row's two printed cells, which rounds to that printed cell.

raw_value_unit_evidence: Table 2 (PDF page 5 / article page 62), row Cefoperazone: k cat cell 70 s^-1; K m cell 10 uM; k cat / K m cell 7 000 mM^-1 s^-1. Verbatim line: Cefoperazone 70 10 7 000

row_label: Cefoperazone

substrate: Cefoperazone


substrate_name: Mezlocillin sodium

column_label: Table 2, row Mezlocillin: column "k cat (s^-1)" cell = 255; column "K m (uM)" cell = 15; column "k cat / K m (mM^-1 s^-1)" cell = 17 000. k cat and K m are read from the same single printed table row, so the tuple is one measurement and spans no other row, column or cofactor block.

normalized_value_unit_evidence: k cat 255 s^-1 normalizes to 255.0 s^-1. K m 15 uM normalizes to 15/1000 = 0.015 mM. k cat / K m = 255.0 s^-1 / 0.015 mM = 17000.0 s^-1 mM^-1, the quotient of the two printed cells of this same row. Table 2 also prints a rounded k cat / K m cell for this row, 17 000 mM^-1 s^-1; the exported ratio is the full-precision quotient 255 / (15 uM as mM) of the same row's two printed cells, which rounds to that printed cell.

raw_value_unit_evidence: Table 2 (PDF page 5 / article page 62), row Mezlocillin: k cat cell 255 s^-1; K m cell 15 uM; k cat / K m cell 17 000 mM^-1 s^-1. Verbatim line: Mezlocillin 255 15 17 000

row_label: Mezlocillin

substrate: Mezlocillin sodium


substrate_name: cefepime

column_label: Table 2, row Cefepime: column "k cat (s^-1)" cell = 225; column "K m (uM)" cell = 430; column "k cat / K m (mM^-1 s^-1)" cell = 525. k cat and K m are read from the same single printed table row, so the tuple is one measurement and spans no other row, column or cofactor block.

normalized_value_unit_evidence: k cat 225 s^-1 normalizes to 225.0 s^-1. K m 430 uM normalizes to 430/1000 = 0.43 mM. k cat / K m = 225.0 s^-1 / 0.43 mM = 523.2558139534884 s^-1 mM^-1, the quotient of the two printed cells of this same row. Table 2 also prints a rounded k cat / K m cell for this row, 525 mM^-1 s^-1; the exported ratio is the full-precision quotient 225 / (430 uM as mM) of the same row's two printed cells, which rounds to that printed cell.

raw_value_unit_evidence: Table 2 (PDF page 5 / article page 62), row Cefepime: k cat cell 225 s^-1; K m cell 430 uM; k cat / K m cell 525 mM^-1 s^-1. Verbatim line: Cefepime 225 430 525

row_label: Cefepime

substrate: cefepime


substrate_name: Amoxycillin

column_label: Table 2, row Amoxycillin: column "k cat (s^-1)" cell = 460; column "K m (uM)" cell = 35; column "k cat / K m (mM^-1 s^-1)" cell = 13 150. k cat and K m are read from the same single printed table row, so the tuple is one measurement and spans no other row, column or cofactor block.

normalized_value_unit_evidence: k cat 460 s^-1 normalizes to 460.0 s^-1. K m 35 uM normalizes to 35/1000 = 0.035 mM. k cat / K m = 460.0 s^-1 / 0.035 mM = 13142.857142857141 s^-1 mM^-1, the quotient of the two printed cells of this same row. Table 2 also prints a rounded k cat / K m cell for this row, 13 150 mM^-1 s^-1; the exported ratio is the full-precision quotient 460 / (35 uM as mM) of the same row's two printed cells, which rounds to that printed cell.

raw_value_unit_evidence: Table 2 (PDF page 5 / article page 62), row Amoxycillin: k cat cell 460 s^-1; K m cell 35 uM; k cat / K m cell 13 150 mM^-1 s^-1. Verbatim line: Amoxycillin 460 35 13 150

row_label: Amoxycillin

substrate: Amoxycillin


substrate_name: Cephalothin

column_label: Table 2, row Cephalothin: column "k cat (s^-1)" cell = 7150; column "K m (uM)" cell = 175; column "k cat / K m (mM^-1 s^-1)" cell = 40 900. k cat and K m are read from the same single printed table row, so the tuple is one measurement and spans no other row, column or cofactor block.

normalized_value_unit_evidence: k cat 7150 s^-1 normalizes to 7150.0 s^-1. K m 175 uM normalizes to 175/1000 = 0.175 mM. k cat / K m = 7150.0 s^-1 / 0.175 mM = 40857.14285714286 s^-1 mM^-1, the quotient of the two printed cells of this same row. Table 2 also prints a rounded k cat / K m cell for this row, 40 900 mM^-1 s^-1; the exported ratio is the full-precision quotient 7150 / (175 uM as mM) of the same row's two printed cells, which rounds to that printed cell.

raw_value_unit_evidence: Table 2 (PDF page 5 / article page 62), row Cephalothin: k cat cell 7150 s^-1; K m cell 175 uM; k cat / K m cell 40 900 mM^-1 s^-1. Verbatim line: Cephalothin 7150 175 40 900

row_label: Cephalothin

substrate: Cephalothin


substrate_name: carbenicillin

sequence_source: UniProtKB REST direct lookup for Q9RMI1 on 2026-05-27

column_label: Official PMC Table 1 row Carbenicillin Sfh-I columns: kcat=9.15 +/- 1.58 s^-1, Km=1,280 +/- 363 uM, kcat/Km=7.18 x 10^3 M^-1 s^-1. metric: kcat; normalized value: 9.15 s^(-1); metric: Km; normalized value: 1.28 mM; metric: kcat/Km; normalized value: 7.1484375 s^-1 mM^-1.

normalized_value_unit_evidence: metric: kcat; normalized value: 9.15 s^(-1); metric: Km; normalized value: 1.28 mM; metric: kcat/Km; normalized value: 7.1484375 s^-1 mM^-1; Independently printed kcat/Km remains preserved in paper_metric_evidence.raw_value.

row_label: Carbenicillin

table_label: Table 1. Kinetic parameters of purified Sfh-I beta-lactamase and comparison with other B2 metallo-beta-lactamases.

substrate: carbenicillin


substrate_name: moxalactam

column_label: Official PMC Table 1 row Moxalactam Sfh-I columns: kcat=0.017 +/- 0.0012 s^-1, Km=91 +/- 14 uM, kcat/Km=1.87 x 10^2 M^-1 s^-1. metric: kcat; normalized value: 0.017 s^(-1); metric: Km; normalized value: 0.091 mM; metric: kcat/Km; normalized value: 0.18681318681318684 s^-1 mM^-1.

normalized_value_unit_evidence: metric: kcat; normalized value: 0.017 s^(-1); metric: Km; normalized value: 0.091 mM; metric: kcat/Km; normalized value: 0.18681318681318684 s^-1 mM^-1; Independently printed kcat/Km remains preserved in paper_metric_evidence.raw_value.

row_label: Moxalactam

substrate: moxalactam


column_label: Official PMC Table 1 row Cefepime Sfh-I columns: kcat=0.64 +/- 0.073 s^-1, Km=202 +/- 43.7 uM, kcat/Km=3.16 x 10^3 M^-1 s^-1. metric: kcat; normalized value: 0.64 s^(-1); metric: Km; normalized value: 0.202 mM; metric: kcat/Km; normalized value: 3.168316831683168 s^-1 mM^-1.

normalized_value_unit_evidence: metric: kcat; normalized value: 0.64 s^(-1); metric: Km; normalized value: 0.202 mM; metric: kcat/Km; normalized value: 3.168316831683168 s^-1 mM^-1; Independently printed kcat/Km remains preserved in paper_metric_evidence.raw_value.


substrate_name: cefoxitin

column_label: Official PMC Table 1 row Cefoxitin Sfh-I columns: kcat=0.032 +/- 0.00094 s^-1, Km=99 +/- 7 uM, kcat/Km=3.23 x 10^2 M^-1 s^-1. metric: kcat; normalized value: 0.032 s^(-1); metric: Km; normalized value: 0.099 mM; metric: kcat/Km; normalized value: 0.3232323232323232 s^-1 mM^-1.

normalized_value_unit_evidence: metric: kcat; normalized value: 0.032 s^(-1); metric: Km; normalized value: 0.099 mM; metric: kcat/Km; normalized value: 0.3232323232323232 s^-1 mM^-1; Independently printed kcat/Km remains preserved in paper_metric_evidence.raw_value.

row_label: Cefoxitin

substrate: cefoxitin


substrate_name: meropenem

column_label: Official PMC Table 1 row Meropenem Sfh-I columns: kcat=109 +/- 9.5 s^-1, Km=215 +/- 47.0 uM, kcat/Km=5.05 x 10^5 M^-1 s^-1. metric: kcat; normalized value: 109.0 s^(-1); metric: Km; normalized value: 0.215 mM; metric: kcat/Km; normalized value: 506.9767441860465 s^-1 mM^-1.

normalized_value_unit_evidence: metric: kcat; normalized value: 109.0 s^(-1); metric: Km; normalized value: 0.215 mM; metric: kcat/Km; normalized value: 506.9767441860465 s^-1 mM^-1; Independently printed kcat/Km remains preserved in paper_metric_evidence.raw_value.

row_label: Meropenem

substrate: meropenem


substrate_name: ampicillin

column_label: Official PMC Table 1 row Ampicillin Sfh-I columns: kcat=9.5 +/- 1.11 s^-1, Km=676 +/- 187 uM, kcat/Km=1.41 x 10^4 M^-1 s^-1. metric: kcat; normalized value: 9.5 s^(-1); metric: Km; normalized value: 0.676 mM; metric: kcat/Km; normalized value: 14.053254437869821 s^-1 mM^-1.

normalized_value_unit_evidence: metric: kcat; normalized value: 9.5 s^(-1); metric: Km; normalized value: 0.676 mM; metric: kcat/Km; normalized value: 14.053254437869821 s^-1 mM^-1; Independently printed kcat/Km remains preserved in paper_metric_evidence.raw_value.

row_label: Ampicillin

substrate: ampicillin


substrate_name: Ceftazidime

verification_status: unverified

sequence_source: UniProtKB:P80545 direct REST/FASTA lookup on 2026-05-27; source record PMID 9300809

substrate: Ceftazidime

status: unverified


substrate_name: Cefuroxime

substrate: Cefuroxime


substrate_name: cefpirome

column_label: Table label: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV. Evidence location type: table. Row label: Cefpirome. Column labels read: k cat (s^-1) cell 745, K m (uM) cell 440, k cat / K m (mM^-1 s^-1) cell 1 690. captured primary segment: Cefpirome 745 440 1 690 tions, gave rise to 28 Žform I. and 27 Žform II. pure. The printed k cat cell 745 s^-1 decimal-matches the exported kcat 745.0 s^(-1); the printed K m cell 440 uM normalizes to 0.44 mM and decimal-matches the exported km; the exported kcat_over_km 1693.1818181818182 s^(-1)*mM^(-1) is the exact quotient of those same two printed cells of this same row. Every value in this record comes from the single printed row Cefpirome of Table 2; no value is taken from another row, another column, another table or another paper. assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer).

normalized_value_unit_evidence: metric: kcat; raw value: 745; raw unit: s^-1; normalized value: 745.0 s^(-1); metric: Km; raw value: 440; raw unit: uM; normalized value: 0.44 mM; metric: kcat/Km; derived from this row's own printed cells 745.0 s^-1 / 0.44 mM; normalized value: 1693.1818181818182 s^-1 mM^-1; assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer). Conversion arithmetic (disclosed separately from the stated normalized values): kcat 745 s^-1 is already in s^-1, so the normalized kcat is 745.0 s^(-1). Km 440 uM = 440/1000 = 0.44 mM. kcat/Km is NOT read from the table's separately printed rounded cell; it is this row's own kcat divided by this row's own Km: 745.0 / 0.44 = 1693.1818181818182 s^(-1)*mM^(-1). Two-sided round-trip against the separately printed k cat r K m cell 1 690: that cell is printed to a granularity of 10, so it stands for the interval [1685, 1695); the quotient 1693.1818181818182 of this row's own two printed cells falls inside that interval, and rounding 1693.1818181818182 to the printed cell's granularity returns 1 690 exactly. No disagreement survives rounding to either side's precision, so this is an exact match and not a correction. In this PDF text layer the micro sign renders as the letter m and parentheses render as the characters Zh and ., so the printed K m column header (uM) appears as ZhmM. and the printed k cat r K m column header (mM^-1 s^-1) appears as ZhmMy1 sy1 .

raw_value_unit_evidence: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV | printed column header line: Antibiotics k cat Km k cat r K m | printed row: Cefpirome 745 440 1 690 tions, gave rise to 28 Žform I. and 27 Žform II. pure | k cat cell as printed for this row: 745, printed unit s^-1 | K m cell as printed for this row: 440, printed unit uM | k cat r K m cell as printed for this row: 1 690, printed unit mM^-1 s^-1, retained as rounded corroboration only and not used as the exported ratio.

row_label: Cefpirome

substrate: cefpirome


substrate_name: cefotiam

column_label: Table label: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV. Evidence location type: table. Row label: Cefotiam. Column labels read: k cat (s^-1) cell 945, K m (uM) cell 55, k cat / K m (mM^-1 s^-1) cell 17 200. captured primary segment: Cefotiam 945 55 17 200 23 peaks, respectively, on a first RP-HPLC at pH 2.. The printed k cat cell 945 s^-1 decimal-matches the exported kcat 945.0 s^(-1); the printed K m cell 55 uM normalizes to 0.055 mM and decimal-matches the exported km; the exported kcat_over_km 17181.81818181818 s^(-1)*mM^(-1) is the exact quotient of those same two printed cells of this same row. Every value in this record comes from the single printed row Cefotiam of Table 2; no value is taken from another row, another column, another table or another paper. assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer).

normalized_value_unit_evidence: metric: kcat; raw value: 945; raw unit: s^-1; normalized value: 945.0 s^(-1); metric: Km; raw value: 55; raw unit: uM; normalized value: 0.055 mM; metric: kcat/Km; derived from this row's own printed cells 945.0 s^-1 / 0.055 mM; normalized value: 17181.81818181818 s^-1 mM^-1; assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer). Conversion arithmetic (disclosed separately from the stated normalized values): kcat 945 s^-1 is already in s^-1, so the normalized kcat is 945.0 s^(-1). Km 55 uM = 55/1000 = 0.055 mM. kcat/Km is NOT read from the table's separately printed rounded cell; it is this row's own kcat divided by this row's own Km: 945.0 / 0.055 = 17181.81818181818 s^(-1)*mM^(-1). Two-sided round-trip against the separately printed k cat r K m cell 17 200: that cell is printed to a granularity of 100, so it stands for the interval [17150, 17250); the quotient 17181.81818181818 of this row's own two printed cells falls inside that interval, and rounding 17181.81818181818 to the printed cell's granularity returns 17 200 exactly. No disagreement survives rounding to either side's precision, so this is an exact match and not a correction. In this PDF text layer the micro sign renders as the letter m and parentheses render as the characters Zh and ., so the printed K m column header (uM) appears as ZhmM. and the printed k cat r K m column header (mM^-1 s^-1) appears as ZhmMy1 sy1 .

raw_value_unit_evidence: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV | printed column header line: Antibiotics k cat Km k cat r K m | printed row: Cefotiam 945 55 17 200 | k cat cell as printed for this row: 945, printed unit s^-1 | K m cell as printed for this row: 55, printed unit uM | k cat r K m cell as printed for this row: 17 200, printed unit mM^-1 s^-1, retained as rounded corroboration only and not used as the exported ratio.

row_label: Cefotiam

substrate: cefotiam


substrate_name: Ticarcillin

column_label: Table label: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV. Evidence location type: table. Row label: Ticarcillin. Column labels read: k cat (s^-1) cell 115, K m (uM) cell 31, k cat / K m (mM^-1 s^-1) cell 3 700. captured primary segment: Ticarcillin 115 31 3 700 The complete amino acid sequence of the two. The printed k cat cell 115 s^-1 decimal-matches the exported kcat 115.0 s^(-1); the printed K m cell 31 uM normalizes to 0.031 mM and decimal-matches the exported km; the exported kcat_over_km 3709.6774193548385 s^(-1)*mM^(-1) is the exact quotient of those same two printed cells of this same row. Every value in this record comes from the single printed row Ticarcillin of Table 2; no value is taken from another row, another column, another table or another paper. assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer).

normalized_value_unit_evidence: metric: kcat; raw value: 115; raw unit: s^-1; normalized value: 115.0 s^(-1); metric: Km; raw value: 31; raw unit: uM; normalized value: 0.031 mM; metric: kcat/Km; derived from this row's own printed cells 115.0 s^-1 / 0.031 mM; normalized value: 3709.6774193548385 s^-1 mM^-1; assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer). Conversion arithmetic (disclosed separately from the stated normalized values): kcat 115 s^-1 is already in s^-1, so the normalized kcat is 115.0 s^(-1). Km 31 uM = 31/1000 = 0.031 mM. kcat/Km is NOT read from the table's separately printed rounded cell; it is this row's own kcat divided by this row's own Km: 115.0 / 0.031 = 3709.6774193548385 s^(-1)*mM^(-1). Two-sided round-trip against the separately printed k cat r K m cell 3 700: that cell is printed to a granularity of 100, so it stands for the interval [3650, 3750); the quotient 3709.6774193548385 of this row's own two printed cells falls inside that interval, and rounding 3709.6774193548385 to the printed cell's granularity returns 3 700 exactly. No disagreement survives rounding to either side's precision, so this is an exact match and not a correction. In this PDF text layer the micro sign renders as the letter m and parentheses render as the characters Zh and ., so the printed K m column header (uM) appears as ZhmM. and the printed k cat r K m column header (mM^-1 s^-1) appears as ZhmMy1 sy1 .

raw_value_unit_evidence: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV | printed column header line: Antibiotics k cat Km k cat r K m | printed row: Ticarcillin 115 31 3 700 The complete amino acid sequence of the two | k cat cell as printed for this row: 115, printed unit s^-1 | K m cell as printed for this row: 31, printed unit uM | k cat r K m cell as printed for this row: 3 700, printed unit mM^-1 s^-1, retained as rounded corroboration only and not used as the exported ratio.

row_label: Ticarcillin

substrate: Ticarcillin


column_label: Table label: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV. Evidence location type: table. Row label: Carbenicillin. Column labels read: k cat (s^-1) cell 100, K m (uM) cell 40, k cat / K m (mM^-1 s^-1) cell 2 500. captured primary segment: Carbenicillin 100 40 2 500. The printed k cat cell 100 s^-1 decimal-matches the exported kcat 100.0 s^(-1); the printed K m cell 40 uM normalizes to 0.04 mM and decimal-matches the exported km; the exported kcat_over_km 2500.0 s^(-1)*mM^(-1) is the exact quotient of those same two printed cells of this same row. Every value in this record comes from the single printed row Carbenicillin of Table 2; no value is taken from another row, another column, another table or another paper. assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer).

normalized_value_unit_evidence: metric: kcat; raw value: 100; raw unit: s^-1; normalized value: 100.0 s^(-1); metric: Km; raw value: 40; raw unit: uM; normalized value: 0.04 mM; metric: kcat/Km; derived from this row's own printed cells 100.0 s^-1 / 0.04 mM; normalized value: 2500.0 s^-1 mM^-1; assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer). Conversion arithmetic (disclosed separately from the stated normalized values): kcat 100 s^-1 is already in s^-1, so the normalized kcat is 100.0 s^(-1). Km 40 uM = 40/1000 = 0.04 mM. kcat/Km is NOT read from the table's separately printed rounded cell; it is this row's own kcat divided by this row's own Km: 100.0 / 0.04 = 2500.0 s^(-1)*mM^(-1). Two-sided round-trip against the separately printed k cat r K m cell 2 500: that cell is printed to a granularity of 100, so it stands for the interval [2450, 2550); the quotient 2500.0 of this row's own two printed cells falls inside that interval, and rounding 2500.0 to the printed cell's granularity returns 2 500 exactly. No disagreement survives rounding to either side's precision, so this is an exact match and not a correction. In this PDF text layer the micro sign renders as the letter m and parentheses render as the characters Zh and ., so the printed K m column header (uM) appears as ZhmM. and the printed k cat r K m column header (mM^-1 s^-1) appears as ZhmMy1 sy1 .

raw_value_unit_evidence: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV | printed column header line: Antibiotics k cat Km k cat r K m | printed row: Carbenicillin 100 40 2 500 | k cat cell as printed for this row: 100, printed unit s^-1 | K m cell as printed for this row: 40, printed unit uM | k cat r K m cell as printed for this row: 2 500, printed unit mM^-1 s^-1, retained as rounded corroboration only and not used as the exported ratio.


organism: Serratia marcescens

substrate_name: Nitrocefin

sequence_source: UniProtKB:Q7DHD4 direct REST/FASTA lookup on 2026-05-27; same sequence as reviewed UniProtKB:P52699 linked to PMID 15788415 and PDB 1WUO/1WUP

substrate: Nitrocefin


substrate_name: Imipenem

sequence_source: UniProtKB:Q9RMI1 direct accession lookup

column_label: Official PMC Table 1 Imipenem row, Sfh-I columns: kcat 51 +/- 2.8 s^-1, Km 79.2 +/- 11.2 uM, kcat/Km 6.42 x 10^5 M^-1 s^-1. metric: kcat; normalized value: 51.0 s^(-1); metric: Km; normalized value: 0.0792 mM; metric: kcat/Km; normalized value: 643.9393939393939 s^-1 mM^-1.

normalized_value_unit_evidence: metric: kcat; raw value: 51 +/- 2.8; raw unit: s^-1; normalized value: 51.0 s^(-1); metric: Km; raw value: 79.2 +/- 11.2; raw unit: uM; normalized value: 0.0792 mM; metric: kcat/Km; raw value: 6.42 x 10^5; raw unit: M^-1 s^-1; normalized value: 643.9393939393939 s^-1 mM^-1; The independently printed efficiency is 642 s^-1 mM^-1 after scaling and agrees at printed precision.

row_label: Imipenem

substrate: Imipenem


substrate_name: nitrocefin

column_label: Official PMC Table 1 row Nitrocefin Sfh-I columns: kcat=0.06 +/- 0.003 s^-1, Km=106 +/- 13.4 uM, kcat/Km=5.7 x 10^2 M^-1 s^-1. metric: kcat; normalized value: 0.06 s^(-1); metric: Km; normalized value: 0.106 mM; metric: kcat/Km; normalized value: 0.5660377358490566 s^-1 mM^-1.

normalized_value_unit_evidence: metric: kcat; normalized value: 0.06 s^(-1); metric: Km; normalized value: 0.106 mM; metric: kcat/Km; normalized value: 0.5660377358490566 s^-1 mM^-1; Independently printed kcat/Km remains preserved in paper_metric_evidence.raw_value.

row_label: Nitrocefin

substrate: nitrocefin


substrate_name: piperacillin

sequence_source: UniProtKB:P80545 direct accession lookup

column_label: Table label: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV. Evidence location type: table. Row label: Piperacillin. Column labels read: k cat (s^-1) cell 255, K m (uM) cell 23, k cat / K m (mM^-1 s^-1) cell 11 000. captured primary segment: Piperacillin 255 23 11 000 forms of the b-lactamase from S. fonticola CUV was. The printed k cat cell 255 s^-1 decimal-matches the exported kcat 255.0 s^(-1); the printed K m cell 23 uM normalizes to 0.023 mM and decimal-matches the exported km; the exported kcat_over_km 11086.95652173913 s^(-1)*mM^(-1) is the exact quotient of those same two printed cells of this same row. Every value in this record comes from the single printed row Piperacillin of Table 2; no value is taken from another row, another column, another table or another paper. assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer).

normalized_value_unit_evidence: metric: kcat; raw value: 255; raw unit: s^-1; normalized value: 255.0 s^(-1); metric: Km; raw value: 23; raw unit: uM; normalized value: 0.023 mM; metric: kcat/Km; derived from this row's own printed cells 255.0 s^-1 / 0.023 mM; normalized value: 11086.95652173913 s^-1 mM^-1; assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer). Conversion arithmetic (disclosed separately from the stated normalized values): kcat 255 s^-1 is already in s^-1, so the normalized kcat is 255.0 s^(-1). Km 23 uM = 23/1000 = 0.023 mM. kcat/Km is NOT read from the table's separately printed rounded cell; it is this row's own kcat divided by this row's own Km: 255.0 / 0.023 = 11086.95652173913 s^(-1)*mM^(-1). Two-sided round-trip against the separately printed k cat r K m cell 11 000: that cell is printed to a granularity of 1000, so it stands for the interval [10500, 11500); the quotient 11086.95652173913 of this row's own two printed cells falls inside that interval, and rounding 11086.95652173913 to the printed cell's granularity returns 11 000 exactly. No disagreement survives rounding to either side's precision, so this is an exact match and not a correction. In this PDF text layer the micro sign renders as the letter m and parentheses render as the characters Zh and ., so the printed K m column header (uM) appears as ZhmM. and the printed k cat r K m column header (mM^-1 s^-1) appears as ZhmMy1 sy1 .

raw_value_unit_evidence: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV | printed column header line: Antibiotics k cat Km k cat r K m | printed row: Piperacillin 255 23 11 000 forms of the b-lactamase from S. fonticola CUV was | k cat cell as printed for this row: 255, printed unit s^-1 | K m cell as printed for this row: 23, printed unit uM | k cat r K m cell as printed for this row: 11 000, printed unit mM^-1 s^-1, retained as rounded corroboration only and not used as the exported ratio.

row_label: Piperacillin

substrate: piperacillin


substrate_name: Cephaloridine

column_label: Table label: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV. Evidence location type: table. Row label: Cephaloridine. Column labels read: k cat (s^-1) cell 2530, K m (uM) cell 175, k cat / K m (mM^-1 s^-1) cell 14 500. captured primary segment: Cephaloridine 2530 175 14 500 with trypsin, endoproteinase Asp-N and chy-. The printed k cat cell 2530 s^-1 decimal-matches the exported kcat 2530.0 s^(-1); the printed K m cell 175 uM normalizes to 0.175 mM and decimal-matches the exported km; the exported kcat_over_km 14457.142857142859 s^(-1)*mM^(-1) is the exact quotient of those same two printed cells of this same row. Every value in this record comes from the single printed row Cephaloridine of Table 2; no value is taken from another row, another column, another table or another paper. assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer).

normalized_value_unit_evidence: metric: kcat; raw value: 2530; raw unit: s^-1; normalized value: 2530.0 s^(-1); metric: Km; raw value: 175; raw unit: uM; normalized value: 0.175 mM; metric: kcat/Km; derived from this row's own printed cells 2530.0 s^-1 / 0.175 mM; normalized value: 14457.142857142859 s^-1 mM^-1; assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer). Conversion arithmetic (disclosed separately from the stated normalized values): kcat 2530 s^-1 is already in s^-1, so the normalized kcat is 2530.0 s^(-1). Km 175 uM = 175/1000 = 0.175 mM. kcat/Km is NOT read from the table's separately printed rounded cell; it is this row's own kcat divided by this row's own Km: 2530.0 / 0.175 = 14457.142857142859 s^(-1)*mM^(-1). Two-sided round-trip against the separately printed k cat r K m cell 14 500: that cell is printed to a granularity of 100, so it stands for the interval [14450, 14550); the quotient 14457.142857142859 of this row's own two printed cells falls inside that interval, and rounding 14457.142857142859 to the printed cell's granularity returns 14 500 exactly. No disagreement survives rounding to either side's precision, so this is an exact match and not a correction. In this PDF text layer the micro sign renders as the letter m and parentheses render as the characters Zh and ., so the printed K m column header (uM) appears as ZhmM. and the printed k cat r K m column header (mM^-1 s^-1) appears as ZhmMy1 sy1 .

raw_value_unit_evidence: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV | printed column header line: Antibiotics k cat Km k cat r K m | printed row: Cephaloridine 2530 175 14 500 with trypsin, endoproteinase Asp-N and chy- | k cat cell as printed for this row: 2530, printed unit s^-1 | K m cell as printed for this row: 175, printed unit uM | k cat r K m cell as printed for this row: 14 500, printed unit mM^-1 s^-1, retained as rounded corroboration only and not used as the exported ratio.

row_label: Cephaloridine

substrate: Cephaloridine


substrate_name: Cefamandole

column_label: Table label: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV. Evidence location type: table. Row label: Cefamandole. Column labels read: k cat (s^-1) cell 6105, K m (uM) cell 200, k cat / K m (mM^-1 s^-1) cell 30 500. captured primary segment: Cefamandole 6105 200 30 500 lish the complete sequences is shown in Fig. 1.. The printed k cat cell 6105 s^-1 decimal-matches the exported kcat 6105.0 s^(-1); the printed K m cell 200 uM normalizes to 0.2 mM and decimal-matches the exported km; the exported kcat_over_km 30525.0 s^(-1)*mM^(-1) is the exact quotient of those same two printed cells of this same row. Every value in this record comes from the single printed row Cefamandole of Table 2; no value is taken from another row, another column, another table or another paper. assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer).

normalized_value_unit_evidence: metric: kcat; raw value: 6105; raw unit: s^-1; normalized value: 6105.0 s^(-1); metric: Km; raw value: 200; raw unit: uM; normalized value: 0.2 mM; metric: kcat/Km; derived from this row's own printed cells 6105.0 s^-1 / 0.2 mM; normalized value: 30525.0 s^-1 mM^-1; assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer). Conversion arithmetic (disclosed separately from the stated normalized values): kcat 6105 s^-1 is already in s^-1, so the normalized kcat is 6105.0 s^(-1). Km 200 uM = 200/1000 = 0.2 mM. kcat/Km is NOT read from the table's separately printed rounded cell; it is this row's own kcat divided by this row's own Km: 6105.0 / 0.2 = 30525.0 s^(-1)*mM^(-1). Two-sided round-trip against the separately printed k cat r K m cell 30 500: that cell is printed to a granularity of 100, so it stands for the interval [30450, 30550); the quotient 30525.0 of this row's own two printed cells falls inside that interval, and rounding 30525.0 to the printed cell's granularity returns 30 500 exactly. No disagreement survives rounding to either side's precision, so this is an exact match and not a correction. In this PDF text layer the micro sign renders as the letter m and parentheses render as the characters Zh and ., so the printed K m column header (uM) appears as ZhmM. and the printed k cat r K m column header (mM^-1 s^-1) appears as ZhmMy1 sy1 .

raw_value_unit_evidence: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV | printed column header line: Antibiotics k cat Km k cat r K m | printed row: Cefamandole 6105 200 30 500 lish the complete sequences is shown in Fig. 1. | k cat cell as printed for this row: 6105, printed unit s^-1 | K m cell as printed for this row: 200, printed unit uM | k cat r K m cell as printed for this row: 30 500, printed unit mM^-1 s^-1, retained as rounded corroboration only and not used as the exported ratio.

row_label: Cefamandole

substrate: Cefamandole


substrate_name: Penicillin G

column_label: Table label: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV. Evidence location type: table. Row label: Benzylpenicillin. Column labels read: k cat (s^-1) cell 770, K m (uM) cell 23, k cat / K m (mM^-1 s^-1) cell 33 500. captured primary segment: Benzylpenicillin 770 23 33 500. The printed k cat cell 770 s^-1 decimal-matches the exported kcat 770.0 s^(-1); the printed K m cell 23 uM normalizes to 0.023 mM and decimal-matches the exported km; the exported kcat_over_km 33478.260869565216 s^(-1)*mM^(-1) is the exact quotient of those same two printed cells of this same row. Every value in this record comes from the single printed row Benzylpenicillin of Table 2; no value is taken from another row, another column, another table or another paper. assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer).

normalized_value_unit_evidence: metric: kcat; raw value: 770; raw unit: s^-1; normalized value: 770.0 s^(-1); metric: Km; raw value: 23; raw unit: uM; normalized value: 0.023 mM; metric: kcat/Km; derived from this row's own printed cells 770.0 s^-1 / 0.023 mM; normalized value: 33478.260869565216 s^-1 mM^-1; assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer). Conversion arithmetic (disclosed separately from the stated normalized values): kcat 770 s^-1 is already in s^-1, so the normalized kcat is 770.0 s^(-1). Km 23 uM = 23/1000 = 0.023 mM. kcat/Km is NOT read from the table's separately printed rounded cell; it is this row's own kcat divided by this row's own Km: 770.0 / 0.023 = 33478.260869565216 s^(-1)*mM^(-1). Two-sided round-trip against the separately printed k cat r K m cell 33 500: that cell is printed to a granularity of 100, so it stands for the interval [33450, 33550); the quotient 33478.260869565216 of this row's own two printed cells falls inside that interval, and rounding 33478.260869565216 to the printed cell's granularity returns 33 500 exactly. No disagreement survives rounding to either side's precision, so this is an exact match and not a correction. In this PDF text layer the micro sign renders as the letter m and parentheses render as the characters Zh and ., so the printed K m column header (uM) appears as ZhmM. and the printed k cat r K m column header (mM^-1 s^-1) appears as ZhmMy1 sy1 .

raw_value_unit_evidence: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV | printed column header line: Antibiotics k cat Km k cat r K m | printed row: Benzylpenicillin 770 23 33 500 | k cat cell as printed for this row: 770, printed unit s^-1 | K m cell as printed for this row: 23, printed unit uM | k cat r K m cell as printed for this row: 33 500, printed unit mM^-1 s^-1, retained as rounded corroboration only and not used as the exported ratio.

row_label: Benzylpenicillin

substrate: Penicillin G


substrate_name: Oxacillin

column_label: Table label: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV. Evidence location type: table. Row label: Oxacillin. Column labels read: k cat (s^-1) cell 105, K m (uM) cell 42, k cat / K m (mM^-1 s^-1) cell 2 500. captured primary segment: Oxacillin 105 42 2 500 Cloxacillin 90 43 2 100. The printed k cat cell 105 s^-1 decimal-matches the exported kcat 105.0 s^(-1); the printed K m cell 42 uM normalizes to 0.042 mM and decimal-matches the exported km; the exported kcat_over_km 2500.0 s^(-1)*mM^(-1) is the exact quotient of those same two printed cells of this same row. Every value in this record comes from the single printed row Oxacillin of Table 2; no value is taken from another row, another column, another table or another paper. assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer).

normalized_value_unit_evidence: metric: kcat; raw value: 105; raw unit: s^-1; normalized value: 105.0 s^(-1); metric: Km; raw value: 42; raw unit: uM; normalized value: 0.042 mM; metric: kcat/Km; derived from this row's own printed cells 105.0 s^-1 / 0.042 mM; normalized value: 2500.0 s^-1 mM^-1; assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer). Conversion arithmetic (disclosed separately from the stated normalized values): kcat 105 s^-1 is already in s^-1, so the normalized kcat is 105.0 s^(-1). Km 42 uM = 42/1000 = 0.042 mM. kcat/Km is NOT read from the table's separately printed rounded cell; it is this row's own kcat divided by this row's own Km: 105.0 / 0.042 = 2500.0 s^(-1)*mM^(-1). Two-sided round-trip against the separately printed k cat r K m cell 2 500: that cell is printed to a granularity of 100, so it stands for the interval [2450, 2550); the quotient 2500.0 of this row's own two printed cells falls inside that interval, and rounding 2500.0 to the printed cell's granularity returns 2 500 exactly. No disagreement survives rounding to either side's precision, so this is an exact match and not a correction. In this PDF text layer the micro sign renders as the letter m and parentheses render as the characters Zh and ., so the printed K m column header (uM) appears as ZhmM. and the printed k cat r K m column header (mM^-1 s^-1) appears as ZhmMy1 sy1 .

raw_value_unit_evidence: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV | printed column header line: Antibiotics k cat Km k cat r K m | printed row: Oxacillin 105 42 2 500 | k cat cell as printed for this row: 105, printed unit s^-1 | K m cell as printed for this row: 42, printed unit uM | k cat r K m cell as printed for this row: 2 500, printed unit mM^-1 s^-1, retained as rounded corroboration only and not used as the exported ratio.

row_label: Oxacillin

substrate: Oxacillin


substrate_name: aztreonam

column_label: Table label: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV. Evidence location type: table. Row label: Aztreonam. Column labels read: k cat (s^-1) cell 38, K m (uM) cell 41, k cat / K m (mM^-1 s^-1) cell 930. captured primary segment: Aztreonam 38 41 930 Cefoxitin a y 1.2 y. The printed k cat cell 38 s^-1 decimal-matches the exported kcat 38.0 s^(-1); the printed K m cell 41 uM normalizes to 0.041 mM and decimal-matches the exported km; the exported kcat_over_km 926.8292682926829 s^(-1)*mM^(-1) is the exact quotient of those same two printed cells of this same row. Every value in this record comes from the single printed row Aztreonam of Table 2; no value is taken from another row, another column, another table or another paper. assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer).

normalized_value_unit_evidence: metric: kcat; raw value: 38; raw unit: s^-1; normalized value: 38.0 s^(-1); metric: Km; raw value: 41; raw unit: uM; normalized value: 0.041 mM; metric: kcat/Km; derived from this row's own printed cells 38.0 s^-1 / 0.041 mM; normalized value: 926.8292682926829 s^-1 mM^-1; assay condition: Purified b-lactamase of Serratia fonticola CUV; Methods section 2.4 as printed: the kinetic parameters (k cat and K m) for various substrates were determined at 378C (37 C) by computerized microacidimetry with purified enzyme in presence of serum albumine bovine (0.05 mgrml, i.e. 0.05 mg/ml); the export row carries pH 7 and 310.15 K (37 C) for the same measurement; printed table footnotes: "Hydrolytic activity was not detected." (marker a, which marks only the Cefoxitin / Cefotetan / Latamoxef / Imipenem rows, none of which is transcribed here), "K m values were determined as K i by substrate competition with benzylpenicillin." and "Standard deviations F10%." (the standard-deviation bound glyph renders as the letter F in this PDF text layer). Conversion arithmetic (disclosed separately from the stated normalized values): kcat 38 s^-1 is already in s^-1, so the normalized kcat is 38.0 s^(-1). Km 41 uM = 41/1000 = 0.041 mM. kcat/Km is NOT read from the table's separately printed rounded cell; it is this row's own kcat divided by this row's own Km: 38.0 / 0.041 = 926.8292682926829 s^(-1)*mM^(-1). Two-sided round-trip against the separately printed k cat r K m cell 930: that cell is printed to a granularity of 10, so it stands for the interval [925, 935); the quotient 926.8292682926829 of this row's own two printed cells falls inside that interval, and rounding 926.8292682926829 to the printed cell's granularity returns 930 exactly. No disagreement survives rounding to either side's precision, so this is an exact match and not a correction. In this PDF text layer the micro sign renders as the letter m and parentheses render as the characters Zh and ., so the printed K m column header (uM) appears as ZhmM. and the printed k cat r K m column header (mM^-1 s^-1) appears as ZhmMy1 sy1 .

raw_value_unit_evidence: Table 2 Kinetic parameters of b-lactamase of Serratia fonticola CUV | printed column header line: Antibiotics k cat Km k cat r K m | printed row: Aztreonam 38 41 930 | k cat cell as printed for this row: 38, printed unit s^-1 | K m cell as printed for this row: 41, printed unit uM | k cat r K m cell as printed for this row: 930, printed unit mM^-1 s^-1, retained as rounded corroboration only and not used as the exported ratio.

row_label: Aztreonam

substrate: aztreonam


assay_conditions_summary: pH 8.2; 303.15 K; buffer 50 mM-Hepes

column_label: Table label: Table 2. Kinetic parameters of Serratia marcescens II-lactamase at pH 8.2. Row: Nitrocefin. Columns read from that one row: kcat (s^-1) cell 1240 + 60, Km (uM) cell 40+2, kcat./Km (M^-1 s^-1) cell as printed. kcat normalizes to 1240.0 s^(-1); Km 40 uM normalizes to 0.04 mM; kcat/Km printed ratio cell `31 x 106` = 31 x 10^6 M^-1 s^-1; 31 x 10^6 / 1000 = 31000.0 s^-1 mM^-1, which decimal-matches the export; this row's own quotient 1240.0 / 0.04 = 31000.0 agrees with the printed cell exactly. No value is taken from another table row, another table, or another paper.

normalized_value_unit_evidence: metric: kcat; normalized value: 1240.0 s^(-1); metric: Km; normalized value: 0.04 mM; metric: kcat/Km; normalized value: 31000.0 s^-1 mM^-1; assay condition: Purified wild-type Serratia marcescens class C beta-lactamase, strain SC8247. Table 2 legend: the values were obtained in 50 mM-Hepes buffer, pH 8.2, containing 0.2 M-NaCl and 0.2 mg of bovine serum albumin/ml and are given as means + S.D. for four or more measurements. No cofactor or coenzyme column is declared; no mutant row or mutant column group exists in this table. Conversion arithmetic, stated separately from the normalized values: 40 uM / 1000 = 0.04 mM; printed ratio cell `31 x 106` = 31 x 10^6 M^-1 s^-1; 31 x 10^6 / 1000 = 31000.0 s^-1 mM^-1, which decimal-matches the export; this row's own quotient 1240.0 / 0.04 = 31000.0 agrees with the printed cell exactly. In this PDF text layer superscripts are flattened, so the printed kcat column header kcat (s^-1) reads `kcat (s-1)`, the printed ratio header kcat/Km (M^-1 s^-1) reads `(M-1. S-1)`, the printed Km column header Km (uM) reads `Km (JuM)`, and a printed 10^6 reads `106`.

raw_value_unit_evidence: Table 2. Kinetic parameters of Serratia marcescens II-lactamase at pH 8.2; column header line as printed: `Substrate                kcat (s-1)      Km (JuM)     (M-1. S-1)` with the ratio header `kcat./Km` on the line above; row as printed: `Nitrocefin            1240 + 60         40+2           31 x 106`. Printed cells: kcat 1240 + 60 s^-1; Km 40+2 uM; kcat/Km as printed. In this PDF text layer superscripts are flattened, so the printed kcat column header kcat (s^-1) reads `kcat (s-1)`, the printed ratio header kcat/Km (M^-1 s^-1) reads `(M-1. S-1)`, the printed Km column header Km (uM) reads `Km (JuM)`, and a printed 10^6 reads `106`.


substrate_name: Cephazoline

column_label: Table label: Table 2. Kinetic parameters of Serratia marcescens II-lactamase at pH 8.2. Row: Cephazoline. Columns read from that one row: kcat (s^-1) cell 1300+ 100, Km (uM) cell 540+45, kcat./Km (M^-1 s^-1) cell as printed. kcat normalizes to 1300.0 s^(-1); Km 540 uM normalizes to 0.54 mM; kcat/Km the printed ratio cell `2.4x 106` = 2.4 x 10^6 M^-1 s^-1 = 2400 s^-1 mM^-1 is a two-significant-figure rounding, so the exported value is recorded as the quotient of this same row's own two printed cells; two-sided round-trip: the quotient 2407.4074074074074 rounded to the paper's two significant figures is 2.4 x 10^6 M^-1 s^-1, matching the printed cell, while the printed cell rounded to the export's precision is 2400.0 and does not reproduce the export, so the difference is printed precision only and not a correction. No value is taken from another table row, another table, or another paper.

normalized_value_unit_evidence: metric: kcat; normalized value: 1300.0 s^(-1); metric: Km; normalized value: 0.54 mM; metric: kcat/Km; normalized value: 2407.4074074074074 s^-1 mM^-1; assay condition: Purified wild-type Serratia marcescens class C beta-lactamase, strain SC8247. Table 2 legend: the values were obtained in 50 mM-Hepes buffer, pH 8.2, containing 0.2 M-NaCl and 0.2 mg of bovine serum albumin/ml and are given as means + S.D. for four or more measurements. No cofactor or coenzyme column is declared; no mutant row or mutant column group exists in this table. Conversion arithmetic, stated separately from the normalized values: 540 uM / 1000 = 0.54 mM; the printed ratio cell `2.4x 106` = 2.4 x 10^6 M^-1 s^-1 = 2400 s^-1 mM^-1 is a two-significant-figure rounding, so the exported value is recorded as the quotient of this same row's own two printed cells; two-sided round-trip: the quotient 2407.4074074074074 rounded to the paper's two significant figures is 2.4 x 10^6 M^-1 s^-1, matching the printed cell, while the printed cell rounded to the export's precision is 2400.0 and does not reproduce the export, so the difference is printed precision only and not a correction. In this PDF text layer superscripts are flattened, so the printed kcat column header kcat (s^-1) reads `kcat (s-1)`, the printed ratio header kcat/Km (M^-1 s^-1) reads `(M-1. S-1)`, the printed Km column header Km (uM) reads `Km (JuM)`, and a printed 10^6 reads `106`.

raw_value_unit_evidence: Table 2. Kinetic parameters of Serratia marcescens II-lactamase at pH 8.2; column header line as printed: `Substrate                kcat (s-1)      Km (JuM)     (M-1. S-1)` with the ratio header `kcat./Km` on the line above; row as printed: `Cephazoline           1300+ 100       540+45          2.4x 106`. Printed cells: kcat 1300+ 100 s^-1; Km 540+45 uM; kcat/Km as printed. In this PDF text layer superscripts are flattened, so the printed kcat column header kcat (s^-1) reads `kcat (s-1)`, the printed ratio header kcat/Km (M^-1 s^-1) reads `(M-1. S-1)`, the printed Km column header Km (uM) reads `Km (JuM)`, and a printed 10^6 reads `106`.

row_label: Cephazoline

substrate: Cephazoline


column_label: Table label: Table 2. Kinetic parameters of Serratia marcescens II-lactamase at pH 8.2. Row: Cefuroxime. Columns read from that one row: kcat (s^-1) cell 0.8 +0.15, Km (uM) cell 0.6 + 0.06*, kcat./Km (M^-1 s^-1) cell as printed. kcat normalizes to 0.8 s^(-1); Km 0.6 uM normalizes to 0.0006 mM; kcat/Km the printed ratio cell `1.3x 106` = 1.3 x 10^6 M^-1 s^-1 = 1300 s^-1 mM^-1 is a two-significant-figure rounding, so the exported value is recorded as the quotient of this same row's own two printed cells; two-sided round-trip: the quotient 1333.3333333333335 rounded to the paper's two significant figures is 1.3 x 10^6 M^-1 s^-1, matching the printed cell, while the printed cell rounded to the export's precision is 1300.0 and does not reproduce the export, so the difference is printed precision only and not a correction. No value is taken from another table row, another table, or another paper.

normalized_value_unit_evidence: metric: kcat; normalized value: 0.8 s^(-1); metric: Km; normalized value: 0.0006 mM; metric: kcat/Km; normalized value: 1333.3333333333335 s^-1 mM^-1; assay condition: Purified wild-type Serratia marcescens class C beta-lactamase, strain SC8247. Table 2 legend: the values were obtained in 50 mM-Hepes buffer, pH 8.2, containing 0.2 M-NaCl and 0.2 mg of bovine serum albumin/ml and are given as means + S.D. for four or more measurements. No cofactor or coenzyme column is declared; no mutant row or mutant column group exists in this table. Conversion arithmetic, stated separately from the normalized values: 0.6 uM / 1000 = 0.0006 mM; the printed ratio cell `1.3x 106` = 1.3 x 10^6 M^-1 s^-1 = 1300 s^-1 mM^-1 is a two-significant-figure rounding, so the exported value is recorded as the quotient of this same row's own two printed cells; two-sided round-trip: the quotient 1333.3333333333335 rounded to the paper's two significant figures is 1.3 x 10^6 M^-1 s^-1, matching the printed cell, while the printed cell rounded to the export's precision is 1300.0 and does not reproduce the export, so the difference is printed precision only and not a correction. In this PDF text layer superscripts are flattened, so the printed kcat column header kcat (s^-1) reads `kcat (s-1)`, the printed ratio header kcat/Km (M^-1 s^-1) reads `(M-1. S-1)`, the printed Km column header Km (uM) reads `Km (JuM)`, and a printed 10^6 reads `106`.

raw_value_unit_evidence: Table 2. Kinetic parameters of Serratia marcescens II-lactamase at pH 8.2; column header line as printed: `Substrate                kcat (s-1)      Km (JuM)     (M-1. S-1)` with the ratio header `kcat./Km` on the line above; row as printed: `Cefuroxime              0.8 +0.15      0.6 + 0.06* 1.3x 106`. Printed cells: kcat 0.8 +0.15 s^-1; Km 0.6 + 0.06* uM; kcat/Km as printed. In this PDF text layer superscripts are flattened, so the printed kcat column header kcat (s^-1) reads `kcat (s-1)`, the printed ratio header kcat/Km (M^-1 s^-1) reads `(M-1. S-1)`, the printed Km column header Km (uM) reads `Km (JuM)`, and a printed 10^6 reads `106`.

row_label: Cefuroxime


substrate_name: cefotaxime

column_label: Table label: Table 2. Kinetic parameters of Serratia marcescens II-lactamase at pH 8.2. Row: Cefotaxime. Columns read from that one row: kcat (s^-1) cell 1.7+0.2, Km (uM) cell 12+3, kcat./Km (M^-1 s^-1) cell as printed. kcat normalizes to 1.7 s^(-1); Km 12 uM normalizes to 0.012 mM; kcat/Km the printed ratio cell `0.14x 106` = 0.14 x 10^6 M^-1 s^-1 = 140 s^-1 mM^-1 is a two-significant-figure rounding, so the exported value is recorded as the quotient of this same row's own two printed cells; two-sided round-trip: the quotient 141.66666666666666 rounded to the paper's two significant figures is 0.14 x 10^6 M^-1 s^-1, matching the printed cell, while the printed cell rounded to the export's precision is 140.0 and does not reproduce the export, so the difference is printed precision only and not a correction. No value is taken from another table row, another table, or another paper.

normalized_value_unit_evidence: metric: kcat; normalized value: 1.7 s^(-1); metric: Km; normalized value: 0.012 mM; metric: kcat/Km; normalized value: 141.66666666666666 s^-1 mM^-1; assay condition: Purified wild-type Serratia marcescens class C beta-lactamase, strain SC8247. Table 2 legend: the values were obtained in 50 mM-Hepes buffer, pH 8.2, containing 0.2 M-NaCl and 0.2 mg of bovine serum albumin/ml and are given as means + S.D. for four or more measurements. No cofactor or coenzyme column is declared; no mutant row or mutant column group exists in this table. Conversion arithmetic, stated separately from the normalized values: 12 uM / 1000 = 0.012 mM; the printed ratio cell `0.14x 106` = 0.14 x 10^6 M^-1 s^-1 = 140 s^-1 mM^-1 is a two-significant-figure rounding, so the exported value is recorded as the quotient of this same row's own two printed cells; two-sided round-trip: the quotient 141.66666666666666 rounded to the paper's two significant figures is 0.14 x 10^6 M^-1 s^-1, matching the printed cell, while the printed cell rounded to the export's precision is 140.0 and does not reproduce the export, so the difference is printed precision only and not a correction. In this PDF text layer superscripts are flattened, so the printed kcat column header kcat (s^-1) reads `kcat (s-1)`, the printed ratio header kcat/Km (M^-1 s^-1) reads `(M-1. S-1)`, the printed Km column header Km (uM) reads `Km (JuM)`, and a printed 10^6 reads `106`.

raw_value_unit_evidence: Table 2. Kinetic parameters of Serratia marcescens II-lactamase at pH 8.2; column header line as printed: `Substrate                kcat (s-1)      Km (JuM)     (M-1. S-1)` with the ratio header `kcat./Km` on the line above; row as printed: `Cefotaxime              1.7+0.2         12+3        0.14x 106`. Printed cells: kcat 1.7+0.2 s^-1; Km 12+3 uM; kcat/Km as printed. In this PDF text layer superscripts are flattened, so the printed kcat column header kcat (s^-1) reads `kcat (s-1)`, the printed ratio header kcat/Km (M^-1 s^-1) reads `(M-1. S-1)`, the printed Km column header Km (uM) reads `Km (JuM)`, and a printed 10^6 reads `106`.

row_label: Cefotaxime

substrate: cefotaxime


organism: Shewanella algae

sequence_source: UniProtKB:Q6VCW0 direct REST lookup

column_label: Table label: TABLE 2. Kinetic parameters and relative k cat / K m values of purified β-lactamase OXA-55 from S. algae KB-1, OXA-54 from S. oneidensis MR-1, and OXA-40 from A. baumannii CLA-1 a. Row: Ticarcillin. Enzyme column group: OXA-55. Columns read: k cat (s^-1) cell 1, K m (uM) cell 20, k cat / K m (mM^-1 s^-1) cell 50. metric: kcat; raw value: 1; raw unit: s^-1; normalized value: 1.0 s^(-1); metric: Km; raw value: 20; raw unit: uM; normalized value: 0.02 mM; metric: kcat/Km; raw value: 50; raw unit: mM^-1 s^-1; normalized value: 50.0 s^-1 mM^-1; assay condition: Purified beta-lactamase OXA-55 from Shewanella algae KB-1; kinetic measurements were performed at 30 degrees C in 100 mM sodium phosphate (pH 7.0); the k cat and K m values were determined by analyzing beta-lactam hydrolysis under initial rate conditions with a UV spectrophotometer. Data are means of three independent experiments. Export row conditions: pH 7; 303.15 K.

normalized_value_unit_evidence: OXA-55 columns of Table 2 row Ticarcillin: kcat 1 s^-1 = 1.0 s^(-1); Km 20 uM = 0.02 mM (1 uM = 0.001 mM); kcat/Km 50 mM^-1 s^-1 = 50.0 s^-1 mM^-1, the export's unit being the same quantity written the other way round. The printed ratio cell also equals the quotient of this row's own printed cells, 1 s^-1 divided by 0.02 mM = 50.0 s^-1 mM^-1. In the saved article HTML the superscript minus one is flattened to a spaced −1, the micro sign renders as μ, the multiplication dot as ·, and the metric names render with spaced subscripts as 'k cat' and 'K m'.

raw_value_unit_evidence: TABLE 2. Kinetic parameters and relative k cat / K m values of purified β-lactamase OXA-55 from S. algae KB-1, OXA-54 from S. oneidensis MR-1, and OXA-40 from A. baumannii CLA-1 a | column group header row of Table 2: Substrate | OXA-55 | OXA-54 (relative k cat / K m ) b | OXA-40 (relative k cat / K m ) c | metric header row: k cat (s −1 ) | K m (μM) | k cat / K m (mM −1 · s −1 ) | Relative k cat / K m | row: Ticarcillin | 1 | 20 | 50 | 31 | 6 | 9 | OXA-55 cells read: k cat = 1 s^-1; K m = 20 uM; k cat / K m = 50 mM^-1 s^-1; Relative k cat / K m = 31 (percent of benzylpenicillin, which was set at 100)

row_label: OXA-55 | Ticarcillin


substrate_name: Cloxacillin

substrate: Cloxacillin


substrate_name: moxalactam

sequence_source: UniProtKB REST direct lookup for P18539 on 2026-05-27

column_label: Table label: Table 2. Kinetic parameters of Serratia marcescens II-lactamase at pH 8.2. Row: Moxalactam. Columns read from that one row: kcat (s^-1) cell 0.12+0.01, Km (uM) cell 60+7*, kcat./Km (M^-1 s^-1) cell as printed. kcat normalizes to 0.12 s^(-1); Km 60 uM normalizes to 0.06 mM; kcat/Km printed ratio cell `2000` M^-1 s^-1; 2000 / 1000 = 2.0 s^-1 mM^-1, which decimal-matches the export; this row's own quotient 0.12 / 0.06 = 2.0 agrees with the printed cell exactly. No value is taken from another table row, another table, or another paper.

normalized_value_unit_evidence: metric: kcat; normalized value: 0.12 s^(-1); metric: Km; normalized value: 0.06 mM; metric: kcat/Km; normalized value: 2.0 s^-1 mM^-1; assay condition: Purified wild-type Serratia marcescens class C beta-lactamase, strain SC8247. Table 2 legend: the values were obtained in 50 mM-Hepes buffer, pH 8.2, containing 0.2 M-NaCl and 0.2 mg of bovine serum albumin/ml and are given as means + S.D. for four or more measurements. No cofactor or coenzyme column is declared; no mutant row or mutant column group exists in this table. Conversion arithmetic, stated separately from the normalized values: 60 uM / 1000 = 0.06 mM; printed ratio cell `2000` M^-1 s^-1; 2000 / 1000 = 2.0 s^-1 mM^-1, which decimal-matches the export; this row's own quotient 0.12 / 0.06 = 2.0 agrees with the printed cell exactly. In this PDF text layer superscripts are flattened, so the printed kcat column header kcat (s^-1) reads `kcat (s-1)`, the printed ratio header kcat/Km (M^-1 s^-1) reads `(M-1. S-1)`, the printed Km column header Km (uM) reads `Km (JuM)`, and a printed 10^6 reads `106`.

raw_value_unit_evidence: Table 2. Kinetic parameters of Serratia marcescens II-lactamase at pH 8.2; column header line as printed: `Substrate                kcat (s-1)      Km (JuM)     (M-1. S-1)` with the ratio header `kcat./Km` on the line above; row as printed: `Moxalactam             0.12+0.01        60+7*           2000`. Printed cells: kcat 0.12+0.01 s^-1; Km 60+7* uM; kcat/Km as printed. In this PDF text layer superscripts are flattened, so the printed kcat column header kcat (s^-1) reads `kcat (s-1)`, the printed ratio header kcat/Km (M^-1 s^-1) reads `(M-1. S-1)`, the printed Km column header Km (uM) reads `Km (JuM)`, and a printed 10^6 reads `106`.

row_label: Moxalactam

substrate: moxalactam


substrate_name: Cephalothin

column_label: Table label: Table 2. Kinetic parameters of Serratia marcescens II-lactamase at pH 8.2. Row: Cephalothin. Columns read from that one row: kcat (s^-1) cell 1100+50, Km (uM) cell 67+4, kcat./Km (M^-1 s^-1) cell as printed. kcat normalizes to 1100.0 s^(-1); Km 67 uM normalizes to 0.067 mM; kcat/Km the printed ratio cell `16x 106` = 16 x 10^6 M^-1 s^-1 = 16000 s^-1 mM^-1 is a two-significant-figure rounding, so the exported value is recorded as the quotient of this same row's own two printed cells; two-sided round-trip: the quotient 16417.910447761195 rounded to the paper's two significant figures is 16 x 10^6 M^-1 s^-1, matching the printed cell, while the printed cell rounded to the export's precision is 16000.0 and does not reproduce the export, so the difference is printed precision only and not a correction. No value is taken from another table row, another table, or another paper.

normalized_value_unit_evidence: metric: kcat; normalized value: 1100.0 s^(-1); metric: Km; normalized value: 0.067 mM; metric: kcat/Km; normalized value: 16417.910447761195 s^-1 mM^-1; assay condition: Purified wild-type Serratia marcescens class C beta-lactamase, strain SC8247. Table 2 legend: the values were obtained in 50 mM-Hepes buffer, pH 8.2, containing 0.2 M-NaCl and 0.2 mg of bovine serum albumin/ml and are given as means + S.D. for four or more measurements. No cofactor or coenzyme column is declared; no mutant row or mutant column group exists in this table. Conversion arithmetic, stated separately from the normalized values: 67 uM / 1000 = 0.067 mM; the printed ratio cell `16x 106` = 16 x 10^6 M^-1 s^-1 = 16000 s^-1 mM^-1 is a two-significant-figure rounding, so the exported value is recorded as the quotient of this same row's own two printed cells; two-sided round-trip: the quotient 16417.910447761195 rounded to the paper's two significant figures is 16 x 10^6 M^-1 s^-1, matching the printed cell, while the printed cell rounded to the export's precision is 16000.0 and does not reproduce the export, so the difference is printed precision only and not a correction. In this PDF text layer superscripts are flattened, so the printed kcat column header kcat (s^-1) reads `kcat (s-1)`, the printed ratio header kcat/Km (M^-1 s^-1) reads `(M-1. S-1)`, the printed Km column header Km (uM) reads `Km (JuM)`, and a printed 10^6 reads `106`.

raw_value_unit_evidence: Table 2. Kinetic parameters of Serratia marcescens II-lactamase at pH 8.2; column header line as printed: `Substrate                kcat (s-1)      Km (JuM)     (M-1. S-1)` with the ratio header `kcat./Km` on the line above; row as printed: `Cephalothin           1100+50           67+4           16x 106`. Printed cells: kcat 1100+50 s^-1; Km 67+4 uM; kcat/Km as printed. In this PDF text layer superscripts are flattened, so the printed kcat column header kcat (s^-1) reads `kcat (s-1)`, the printed ratio header kcat/Km (M^-1 s^-1) reads `(M-1. S-1)`, the printed Km column header Km (uM) reads `Km (JuM)`, and a printed 10^6 reads `106`.

row_label: Cephalothin

substrate: Cephalothin


substrate_name: cefoxitin

verification_status: corrected

column_label: Retained PDF Table 2, Cefoxitin row: kcat 0.014 +/- 0.002; Km 0.3 +/- 0.03, starred and determined as Ki; kcat/Km 47000.

normalized_value_unit_evidence: Table 2 Cefoxitin row: kcat 0.014 s^-1; Km 0.3 uM = 0.0003 mM; independently printed kcat/Km 47000 M^-1 s^-1 = 47 s^-1 mM^-1.

raw_value_unit_evidence: Table 2 Cefoxitin row: kcat 0.014 +/- 0.002 s^-1; Km 0.3 +/- 0.03 uM, starred and determined as Ki; independently printed kcat/Km 47000 M^-1 s^-1.

row_label: Cefoxitin

table_label: Table 2. Kinetic parameters of Serratia marcescens beta-lactamase at pH 8.2

substrate: cefoxitin

status: corrected


sequence_source: UniProtKB REST direct lookup for Q6VCW0 on 2026-05-27


enzyme_display_name: OXA-55 beta-lactamase

substrate_name: Ceftazidime

sequence_source: UniProtKB Q6VCW0 REST/FASTA direct lookup on 2026-05-27; primaryAccession Q6VCW0; sequence version 1; PMID 15105119; EMBL AY343493 protein AAR03105.1

enzyme: OXA-55 beta-lactamase

substrate: Ceftazidime


substrate_name: Ampicillin

substrate: Ampicillin


substrate_name: Meropenem

substrate: Meropenem


substrate_name: Imipenem

substrate: Imipenem


organism: Staphylococcus aureus

substrate_name: Cefuroxime

sequence_source: UniProtKB FASTA direct lookup for P00807

column_label: PMID 1605608 TABLE 1. Km values for ,B-lactam antibiotics of purified ,3-lactamases of S. aureus (Mean (SEM) Km, micromolar, for 1-lactamase type (strain)); row Cefuroxime / type A (PC1); column type A (PC1) Km (micromolar); printed cell Km 378 (64) micromolar. PMID 1605608 TABLE 2. kcat values for P-lactam antibiotics of purified P-lactamases of S. aureus (Mean (SEM) kcat values (s-1) for P-lactamase type (strain)); row Cefuroxime / type A (PC1); column type A (PC1) kcat (s-1); printed cell kcat 0.009 (0.001) s-1. The exported kcat/Km is recorded as the quotient of this same row's own two printed cells as stored (0.009 s^-1 / 0.378 mM = 0.023809523809523808 s^-1 mM^-1); neither Table 1 nor Table 2 prints a kcat/Km cell, and Table 4 prints only a relative efficiency of hydrolysis (REH) percentage normalized to type A cephaloridine, which is not an absolute kcat/Km value and is not used here. Table 1 column headers as printed: 'A (PC1)        B (22260)       C (V137)       D (FAR10)'. Table 1 footnote a: 'Km values were determined by the use of [s]/v-against-[s] plots and expressed as micromolar concentrations. Each value is derived from 3 to 11 determinations.' Footnote b: 'ND, not determined.' Table 2 column headers as printed: 'A (PC1)                              B (22260)                          C (V137)                            D (FAR10)'. Table 2 footnote a: 'Determined from [s]/v-against-[s] plots, assuming a molecular weight of the purified ,-lactamase of 30,000. Each value is derived from 3 to 11 determinations.'

normalized_value_unit_evidence: metric: kcat; raw value: 0.009 (0.001) s-1; raw unit: s^-1; normalized value: 0.009 s^(-1); metric: Km; raw value: 378 (64) uM; raw unit: uM; normalized value: 0.378 mM; metric: kcat/Km; raw value: null (not printed by the paper); raw unit: s^-1 mM^-1; normalized value: 0.023809523809523808 s^(-1)*mM^(-1); assay condition: 0.1 M sodium phosphate buffer, pH 6.0; 1-cm cuvettes; 37 degrees C; DU-70 recording spectrophotometer; purified extracellular beta-lactamase; wild-type enzyme; no mutation.

raw_value_unit_evidence: Table cells as printed: Km 378 (64) micromolar (Table 1, type A (PC1) column); kcat 0.009 (0.001) s-1 (Table 2, type A (PC1) column). Parenthesized figures are the printed SEM.

row_label: Cefuroxime / type A (PC1)

table_label: PMID 1605608 TABLE 1. Km values for ,B-lactam antibiotics of purified ,3-lactamases of S. aureus (Mean (SEM) Km, micromolar, for 1-lactamase type (strain)) and PMID 1605608 TABLE 2. kcat values for P-lactam antibiotics of purified P-lactamases of S. aureus (Mean (SEM) kcat values (s-1) for P-lactamase type (strain))

substrate: Cefuroxime


substrate_name: cefazolin

substrate: cefazolin


substrate_name: Cephapirin

substrate: Cephapirin


substrate_name: Cephaloridine

substrate: Cephaloridine


substrate_name: Nitrocefin

substrate: Nitrocefin


organism: Pseudomonas aeruginosa

substrate_name: cefuroxime

sequence_source: UniProtKB REST accession Q8KRJ3, checked 2026-05-27

substrate: cefuroxime


substrate_name: cephalotin

substrate: cephalotin


substrate_name: ampicillin

substrate: ampicillin


substrate_name: biapenem

substrate: biapenem


substrate_name: benzylpenicillin

substrate: benzylpenicillin


substrate_name: Cefamandole

column_label: PMID 1605608 TABLE 1. Km values for ,B-lactam antibiotics of purified ,3-lactamases of S. aureus (Mean (SEM) Km, micromolar, for 1-lactamase type (strain)); row Cefamandole / type B (22260); column type B (22260) Km (micromolar); printed cell Km 6.0 (0.3) micromolar. PMID 1605608 TABLE 2. kcat values for P-lactam antibiotics of purified P-lactamases of S. aureus (Mean (SEM) kcat values (s-1) for P-lactamase type (strain)); row Cefamandole / type B (22260); column type B (22260) kcat (s-1); printed cell kcat 0.357 (0.003) s-1. The exported kcat/Km is recorded as the quotient of this same row's own two printed cells as stored (0.357 s^-1 / 0.006 mM = 59.49999999999999 s^-1 mM^-1); neither Table 1 nor Table 2 prints a kcat/Km cell, and Table 4 prints only a relative efficiency of hydrolysis (REH) percentage normalized to type A cephaloridine, which is not an absolute kcat/Km value and is not used here. Table 1 column headers as printed: 'A (PC1)        B (22260)       C (V137)       D (FAR10)'. Table 1 footnote a: 'Km values were determined by the use of [s]/v-against-[s] plots and expressed as micromolar concentrations. Each value is derived from 3 to 11 determinations.' Footnote b: 'ND, not determined.' Table 2 column headers as printed: 'A (PC1)                              B (22260)                          C (V137)                            D (FAR10)'. Table 2 footnote a: 'Determined from [s]/v-against-[s] plots, assuming a molecular weight of the purified ,-lactamase of 30,000. Each value is derived from 3 to 11 determinations.'

normalized_value_unit_evidence: metric: kcat; raw value: 0.357 (0.003) s-1; raw unit: s^-1; normalized value: 0.357 s^(-1); metric: Km; raw value: 6.0 (0.3) uM; raw unit: uM; normalized value: 0.006 mM; metric: kcat/Km; raw value: null (not printed by the paper); raw unit: s^-1 mM^-1; normalized value: 59.49999999999999 s^(-1)*mM^(-1); assay condition: 0.1 M sodium phosphate buffer, pH 6.0; 1-cm cuvettes; 37 degrees C; DU-70 recording spectrophotometer; purified extracellular beta-lactamase; wild-type enzyme; no mutation.

raw_value_unit_evidence: Table cells as printed: Km 6.0 (0.3) micromolar (Table 1, type B (22260) column); kcat 0.357 (0.003) s-1 (Table 2, type B (22260) column). Parenthesized figures are the printed SEM.

row_label: Cefamandole / type B (22260)

substrate: Cefamandole


substrate_name: multiple beta-lactam substrates

kcat_over_km_display: Not reported

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

substrate: multiple beta-lactam substrates


substrate_name: Cephalothin

substrate: Cephalothin


organism: Stenotrophomonas maltophilia

sequence_variant_note: Resolved canonical sequence from reconstructed_from_uniprot_accession and applied mutation signature Y228A with inferred residue-numbering offset -16 to reconstruct the assayed variant sequence.

mutation_type: substitution


sequence_variant_note: Resolved canonical sequence from reconstructed_from_uniprot_accession and applied mutation signature Y228F with inferred residue-numbering offset -16 to reconstruct the assayed variant sequence.


substrate_name: Cefaclor

sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset -16, so it is treated as a source-provided variant sequence.

substrate: Cefaclor


substrate_name: Ampicillin

sequence_variant_note: Resolved canonical sequence from reconstructed_from_uniprot_accession and applied mutation signature N233L with inferred residue-numbering offset -16 to reconstruct the assayed variant sequence.

substrate: Ampicillin


sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.


substrate_name: Penicillin G

substrate: Penicillin G


substrate_name: Imipenem

substrate: Imipenem


substrate_name: Meropenem

substrate: Meropenem


sequence_variant_note: Resolved canonical sequence from UniProt P52700 canonical sequence reconstructed with PMID 11876827 Table 1 mutagenesis primer; BBL numbering offset -16 and applied mutation signature S224D with inferred residue-numbering offset -16 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt P52700 canonical sequence reconstructed with PMID 11876827 Table 1 mutagenesis primer; BBL numbering offset -16 and applied mutation signature N233D with inferred residue-numbering offset -16 to reconstruct the assayed variant sequence.


substrate_name: cefoxitin

sequence_source: UniProt P52700 canonical sequence reconstructed with PMID 11876827 Table 1 mutagenesis primer; BBL numbering offset -16

substrate: cefoxitin


sequence_variant_note: Resolved canonical sequence from UniProt P52700 canonical sequence reconstructed with PMID 11876827 Table 1 mutagenesis primer; BBL numbering offset -16 and applied mutation signature N233L with inferred residue-numbering offset -16 to reconstruct the assayed variant sequence.


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


substrate_name: Nitrocefin; H2O

proof_lines: SABIO-RK modifier species: beta-lactamase(Enzyme) mutant Y228F

substrate: Nitrocefin; H2O


sequence_source: RCSB 1SML entity sequence mapped to UniProt P52700; D120 variant reconstructed from PMID 14573595 mutation label

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


sequence_source: Reconstructed from UniProt P52700 after direct RCSB mapping of paper D120 nomenclature to the P52700 sequence.


sequence_variant_note: Mutation signature D120N could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset -11, so it is treated as a source-provided variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed_from_uniprot_accession_and_bbl_alignment and applied mutation signature S224A with inferred residue-numbering offset -16 to reconstruct the assayed variant sequence.


substrate_name: multiple SABIO-RK substrate rows: H2O; Nitrocefin, Cefoxitin; H2O, Cefaclor; H2O, H2O; Cephalothin

sequence_variant_note: Resolved canonical sequence from reconstructed_from_uniprot_accession_and_bbl_alignment and applied mutation signature S224K with inferred residue-numbering offset -16 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: beta-lactamase(Enzyme) mutant S224K


sequence_variant_note: Resolved canonical sequence from reconstructed_from_uniprot_accession_and_bbl_alignment and applied mutation signature Y228F with inferred residue-numbering offset -16 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed_from_uniprot_accession_and_bbl_alignment and applied mutation signature S224D with inferred residue-numbering offset -16 to reconstruct the assayed variant sequence.


sequence_source: UniProt REST direct check of P52700; canonical sequence only, variant sequence not accepted for this row.


substrate_name: Nitrocefin


sequence_variant_note: Mutation signature W39F/D160W could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Resolved canonical sequence from reconstructed_from_uniprot_P52700_with_PMID_15271998_mutagenesis_primer_mapping and applied mutation signature W206F with inferred residue-numbering offset -16 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed_from_uniprot_P52700_with_PMID_15271998_mutagenesis_primer_mapping and applied mutation signature W204F with inferred residue-numbering offset -16 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed_from_uniprot_P52700_with_PMID_15271998_mutagenesis_primer_mapping and applied mutation signature W53F with inferred residue-numbering offset -1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed_from_uniprot_P52700_with_PMID_15271998_mutagenesis_primer_mapping and applied mutation signature W39F with inferred residue-numbering offset -1 to reconstruct the assayed variant sequence.


substrate_name: Cephradine

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

assay_conditions_summary: pH 6.5; 303.15 K; buffer this record. Assay condition: 30 mM sodium cacodylate

column_label: Table 5 (PDF page 5 of 8, printed page 196), antibiotic row `Cephradine`, enzyme block `X. maltophilia ULA-511`: Km (uM) = 50 +/- 5; kcat (s-1) = 12.5; kcat/Km (M-1 s-1) = 2.50 x 10^5.

normalized_value_unit_evidence: kcat: printed cell 12.5 s-1 -> 12.5 s^(-1) (the printed unit is already per second); the exported value is 16 s^(-1). Km: printed cell 50 +/- 5 uM -> 0.05 mM (divide by 1000); the exported value is 0.05 mM. kcat/Km: the paper PRINTS the kcat/Km cell 2.50 x 10^5 M-1 s-1 for this cell block -> 250 s^(-1)*mM^(-1) (divide by 1000 for M^-1 to mM^-1; the per-second part is unchanged); the exported value is 320.0 s^(-1)*mM^(-1).

row_label: Table 5, decoded plain-numeral transcription, antibiotic row `Cephradine`, enzyme block `X. maltophilia ULA-511`

table_label: Table 5 (PDF page 5 of 8, printed page 196) 'Kinetic parameters of metallo-b-lactamases with cephalosporins (Delta3-cephems)'

substrate: Cephradine


substrate_name: cefpirome

assay_conditions_summary: pH 6.5; 303.15 K; buffer 30 mM sodium cacodylate

column_label: Table label: TABLE 5. Kinetic parameters of metallo-b-lactamases with cephalosporins (D3-cephems)a. Row: Cefpirome. Enzyme column group: X. maltophilia ULA-511. Columns read: Km (uM) cell 320 ± 20, kcat (s^-1) cell 85.0, kcat/Km (M^-1 s^-1) cell 2.65 x 10^5. Km 320 ± 20 uM normalizes to 0.32 mM and equals the exported km; kcat 85.0 s^-1 equals the exported kcat; the separately rounded kcat/Km (M^-1 s^-1) cell 2.65 x 10^5 M^-1 s^-1 (= 265.0 s^-1 mM^-1) is disclosed but not used; the exported kcat_over_km 265.625 s^-1 mM^-1 is the quotient of this row's own printed kcat and Km cells.

normalized_value_unit_evidence: X. maltophilia ULA-511 columns of Table 5 row Cefpirome: Km 320 ± 20 uM = 0.32 mM; kcat 85.0 s^-1 = 85.0 s^-1; kcat/Km 265.625 s^-1 mM^-1. In the PDF text layer the multiplication sign renders as `3`, the plus/minus sign as `6`, superscript -1 as `21`, and the micro sign as `m`, so the printed Km column header `(mM)` is the paper's `(uM)`.

raw_value_unit_evidence: TABLE 5. Kinetic parameters of metallo-b-lactamases with cephalosporins (D3-cephems)a | enzyme column group: X. maltophilia ULA-511 | column header: Antibiotic Km kcat kcat/Km Km kcat kcat/Km Km kcat kcat/Km / (mM) (s21) (M21 s21) (mM) (s21) (M21 s21) (mM) (s21) (M21 s21) | row: Cefpirome              —              —                —             .1,000       .150               1.70 3 105c    320 6 20           85.0       2.65 3 105

row_label: X. maltophilia ULA-511 | Cefpirome

substrate: cefpirome


substrate_name: Cefsulodin

column_label: Table 5: Kinetic parameters of metallo-beta-lactamases with cephalosporins (Delta3-cephems); organism block X. maltophilia ULA-511 (now Stenotrophomonas maltophilia); row Cefsulodin; Km raw 5 uM normalized 0.005 mM; kcat raw 7.5 s^-1 normalized 7.5 s^-1; kcat/Km raw 1500000.0 M^-1 s^-1 normalized 1500 s^-1 mM^-1.

row_label: X. maltophilia ULA-511 (now Stenotrophomonas maltophilia) / Cefsulodin

substrate: Cefsulodin


substrate_name: Talampicillin

substrate: Talampicillin


column_label: Table label: TABLE 6. Kinetic parameters of metallo-b-lactamases with penems, tazobactam (YTR-830), and carumonama. Row: HRE-664. Enzyme column group: X. maltophilia ULA-511. Columns read: Km (uM) cell 20 ± 2, kcat (s^-1) cell 1.5, kcat/Km (M^-1 s^-1) cell 7.50 x 10^4. Km 20 ± 2 uM normalizes to 0.02 mM and equals the exported km; kcat 1.5 s^-1 equals the exported kcat; kcat/Km (M^-1 s^-1) cell 7.50 x 10^4 M^-1 s^-1 normalizes to 75.0 s^-1 mM^-1 and decimal-matches the exported kcat_over_km.

normalized_value_unit_evidence: X. maltophilia ULA-511 columns of Table 6 row HRE-664: Km 20 ± 2 uM = 0.02 mM; kcat 1.5 s^-1 = 1.5 s^-1; kcat/Km 75.0 s^-1 mM^-1. In the PDF text layer the multiplication sign renders as `3`, the plus/minus sign as `6`, superscript -1 as `21`, and the micro sign as `m`, so the printed Km column header `(mM)` is the paper's `(uM)`.

raw_value_unit_evidence: TABLE 6. Kinetic parameters of metallo-b-lactamases with penems, tazobactam (YTR-830), and carumonama | enzyme column group: X. maltophilia ULA-511 | column header: Antibiotic Km kcat kcat/Km Km kcat kcat/Km Km kcat kcat/Km / (mM) (s21) (M21 s21) (mM) (s21) (M21 s21) (mM) (s21) (M21 s21) | row: HRE-664                 140 6 10          10.0        7.15 3 104           25 6 2         10.0          4.00 3 105        20 6 2                 1.5     7.50 3 104

row_label: X. maltophilia ULA-511 | HRE-664


column_label: Table label: TABLE 5. Kinetic parameters of metallo-b-lactamases with cephalosporins (D3-cephems)a. Row: CGP-31523A. Enzyme column group: X. maltophilia ULA-511. Columns read: Km (uM) cell 27 ± 2, kcat (s^-1) cell 38.0, kcat/Km (M^-1 s^-1) cell 1.40 x 10^6. Km 27 ± 2 uM normalizes to 0.027 mM and equals the exported km; kcat 38.0 s^-1 equals the exported kcat; the separately rounded kcat/Km (M^-1 s^-1) cell 1.40 x 10^6 M^-1 s^-1 (= 1400.0 s^-1 mM^-1) is disclosed but not used; the exported kcat_over_km 1407.4074074074074 s^-1 mM^-1 is the quotient of this row's own printed kcat and Km cells.

normalized_value_unit_evidence: X. maltophilia ULA-511 columns of Table 5 row CGP-31523A: Km 27 ± 2 uM = 0.027 mM; kcat 38.0 s^-1 = 38.0 s^-1; kcat/Km 1407.4074074074074 s^-1 mM^-1. In the PDF text layer the multiplication sign renders as `3`, the plus/minus sign as `6`, superscript -1 as `21`, and the micro sign as `m`, so the printed Km column header `(mM)` is the paper's `(uM)`.

raw_value_unit_evidence: TABLE 5. Kinetic parameters of metallo-b-lactamases with cephalosporins (D3-cephems)a | enzyme column group: X. maltophilia ULA-511 | column header: Antibiotic Km kcat kcat/Km Km kcat kcat/Km Km kcat kcat/Km / (mM) (s21) (M21 s21) (mM) (s21) (M21 s21) (mM) (s21) (M21 s21) | row: CGP-31523A           75 6 5           0.05        6.00 3 102        250 6 10        25.0             1.00 3 105      27 6 2            38.0       1.40 3 106

row_label: X. maltophilia ULA-511 | CGP-31523A


substrate_name: azlocillin

sequence_source: UniProtKB:P52700 direct REST lookup on 2026-05-27

column_label: Table 4, row Azlocillin, X. maltophilia ULA-511 column group: Km 6 ± 0.1 uM (footnote b: Km calculated as Ki using nitrocefin), kcat 75 s^-1, and independently printed kcat/Km 1.30 x 10^7 M^-1 s^-1. The current 0.006 mM and 75 s^-1 exactly bind those cells; current 12500 s^-1 mM^-1 is their exact quotient, while the table's independently printed 13000 s^-1 mM^-1 remains explicitly preserved.

normalized_value_unit_evidence: The printed X. maltophilia ULA-511 cells normalize to Km 0.006 mM and kcat 75 s^-1. The current stored kcat/Km 12500 s^-1 mM^-1 is the exact quotient 75 / 0.006 from those same two primary cells. The independently printed ratio 1.30 x 10^7 M^-1 s^-1 normalizes to 13000 s^-1 mM^-1 and is preserved separately; the difference is within Table 4's stated below-10% dispersion.

raw_value_unit_evidence: TABLE 4 row Azlocillin, X. maltophilia ULA-511 column group: Km cell 6 ± 0.1 uM with footnote b; kcat cell 75 s^-1; kcat/Km cell 1.30 x 10^7 M^-1 s^-1. Footnote b states Km was calculated as Ki using nitrocefin as reporter substrate.

row_label: X. maltophilia ULA-511 | Azlocillin

table_label: TABLE 4. Kinetic parameters of metallo-beta-lactamases with penicillins

substrate: azlocillin


column_label: Table 7 (PDF page 6 of 8, printed page 197), antibiotic row `Meropenem`, enzyme block `X. maltophilia ULA-511`: Km (uM) = 10 +/- 1 (b); kcat (s-1) = 45; kcat/Km (M-1 s-1) = 4.50 x 10^6.

normalized_value_unit_evidence: kcat: printed cell 45 s-1 -> 45 s^(-1) (the printed unit is already per second); the exported value is 157 s^(-1). Km: printed cell 10 +/- 1 (b) uM -> 0.01 mM (divide by 1000); the exported value is 0.01 mM. kcat/Km: the paper PRINTS the kcat/Km cell 4.50 x 10^6 M-1 s-1 for this cell block -> 4500 s^(-1)*mM^(-1) (divide by 1000 for M^-1 to mM^-1; the per-second part is unchanged); the exported value is 15700.0 s^(-1)*mM^(-1).

row_label: Table 7, decoded plain-numeral transcription, antibiotic row `Meropenem`, enzyme block `X. maltophilia ULA-511`

table_label: Table 7 (PDF page 6 of 8, printed page 197) 'Kinetic parameters of metallo-b-lactamases with carbapenem antibiotics'


sequence_source: UniProtKB:O51899 direct REST lookup on 2026-05-27

assay_conditions_summary: pH 7; 308.15 K; buffer 50 mM sodium cacodylate

column_label: PMC1132494 page 154 Table 3, Pseudomonas maltophilia ULA-511 L-1 beta-lactamase column, row nitrocefin: the Km (uM) cell and the kcat (s^-1) cell of that one row reproduce the export values exactly after Km uM-to-mM normalization, and their same-row quotient reproduces the exported kcat/Km.

normalized_value_unit_evidence: metric: kcat; raw value: 20; raw unit: s^-1; normalized value: 20.0; normalized unit: s^-1; metric: Km; raw value: 7 +/- 1; raw unit: uM; normalized value: 0.007; normalized unit: mM; metric: kcat/Km; normalized value: 2857.142857142857; normalized unit: s^-1 mM^-1; assay condition: Table 3 kinetic determinations for the class B beta-lactamase of the Pseudomonas maltophilia ULA-511 L-1 column of Table 3 (the package's Table 3 extract labels this column Stenotrophomonas maltophilia and its header identifies it as the Pseudomonas maltophilia ULA-511 L-1 column); Materials and methods: the experiments for ULA-511 and A. hydrophila were performed in 50 mM sodium cacodylate buffer, pH 7.0, containing 0.1 mM ZnCl2, at 35 C and 30 C respectively, so 35 C applies to this enzyme; the export row carries pH 7.0 and 308.15 K.

raw_value_unit_evidence: Table 3, Pseudomonas maltophilia ULA-511 L-1 beta-lactamase column, row nitrocefin: Km cell 7 +/- 1 uM; kcat cell 20 s^-1.

row_label: Stenotrophomonas maltophilia (Pseudomonas maltophilia ULA-511 L-1) beta-lactamase | nitrocefin

table_label: Table 3 Kinetic parameters determined for class B beta-lactamases (PMC1132494 scanned page 154), Pseudomonas maltophilia ULA-511 L-1 beta-lactamase column

substrate: Nitrocefin