enzyme_display_name: D-2-hydroxyacid dehydrogenase (NAD(+))

organism: Lactobacillus delbrueckii subsp. bulgaricus

substrate_name: phenylpyruvate

proof_lines: recombinant His-tagged enzyme, pH and temperature not specified in the publication

enzyme: D-2-hydroxyacid dehydrogenase (NAD(+))

substrate: phenylpyruvate


enzyme_display_name: ferredoxin--nitrite reductase

organism: Cyanidioschyzon merolae

substrate_name: nitrite

source_db: brenda

ki_display: Not reported

kcat_over_km_display: Not reported

protein_accession_database: UniProt

source_license: CC BY 4.0

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: mutant G212S/L213T/Y214L/S217C/C220I/S221N, pH not specified in the publication, temperature not specified in the publication

enzyme: ferredoxin--nitrite reductase

substrate: nitrite

source: brenda


mutation_signature: truncated protein

mutation_type: truncation

proof_lines: truncated protein, residues 19-731, pH not specified in the publication, temperature not specified in the publication


proof_lines: truncated protein, residues 76-679, pH not specified in the publication, temperature not specified in the publication


organism: Nicotiana tabacum

mutation_type: substitution


source_db: oed

source: oed


mutation_signature: Unknown

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


enzyme_display_name: cyclohexanone monooxygenase

organism: Rhodococcus sp. HI-31

substrate_name: NADPH

kcat_display: Not reported

proof_lines: mutant W492A, 0.1 mM cyclohexanone, 50 mM Tris-HCl buffer (pH 9.0), 25°C

enzyme: cyclohexanone monooxygenase

substrate: NADPH


substrate_name: cyclohexanone

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

proof_lines: wild-type, pH 7.5, temperature not specified in the publication

proof_lines: wild type enzyme, 0.1 mM cyclohexanone, 50 mM Tris-HCl buffer (pH 9.0), 25°C

substrate: cyclohexanone


km_display: Not reported

proof_lines: mutant E91Q/D95H/A115V/R280Y/N431Y/Q439P/A455V/S534R, pH 7.5, temperature not specified in the publication


organism: Thermocrispum municipale

substrate_name: phenylacetone

substrate: phenylacetone


substrate_name: acetophenone

source_license: CC BY 4.0; CC BY-NC-ND 4.0

proof_lines:
pH 7.0, 25°C
Raw SKiD Env: Temp=25°C, pH=7

source_databases_merged: brenda skid

substrate: acetophenone


substrate_name: Cyclopentanone

substrate: Cyclopentanone


smiles: CCC(C)=O


organism: Lacticaseibacillus paracasei


organism: Acinetobacter sp.

assay_conditions_summary: pH 7.7; 298.15 K; cofactor NADPH


organism: Ilyonectria destructans

substrate_name: Cyclobutanone

substrate: Cyclobutanone


substrate_name: Cyclooctanone

substrate: Cyclooctanone


substrate_name: Cycloheptanone

substrate: Cycloheptanone


organism: Synechocystis sp. PCC 6803

proof_lines: cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication

proof_lines: cosubstrate Fdx2, pH 7.5, temperature not specified in the publication


enzyme_display_name: glutathionyl-hydroquinone reductase

organism: Escherichia coli

substrate_name: glutathione

enzyme: glutathionyl-hydroquinone reductase

substrate: glutathione


enzyme_display_name: 1,3-beta-D-glucan phosphorylase

organism: Euglena gracilis var. saccharophila

substrate_name: laminaritetraose

enzyme: 1,3-beta-D-glucan phosphorylase

substrate: laminaritetraose


substrate_name: alpha-D-glucose

substrate: alpha-D-glucose


substrate_name: Laminarihexaose

substrate: Laminarihexaose


substrate_name: laminaribiose

substrate: laminaribiose


substrate_name: laminaritriose

substrate: laminaritriose


substrate_name: Laminaripentaose

substrate: Laminaripentaose


organism: Paenibacillus polymyxa

proof_lines: pH and temperature not specified in the publication


organism: Pyrococcus horikoshii

substrate_name: S-adenosyl-L-methionine

proof_lines:
at pH 8.5 and 37°C
Raw SKiD Env: Temp=37°C, pH=8.5

substrate: S-adenosyl-L-methionine


organism: Salinispora tropica

substrate_name: (R)-S-adenosyl-L-methionine

substrate: (R)-S-adenosyl-L-methionine


enzyme_display_name: selenocysteine lyase

organism: Arabidopsis thaliana

substrate_name: L-Selenocysteine; Reduced acceptor

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

enzyme: selenocysteine lyase

substrate: L-Selenocysteine; Reduced acceptor


enzyme_display_name: assimilatory sulfite reductase (ferredoxin)

substrate_name: sulfite

substrate: sulfite


enzyme: assimilatory sulfite reductase (ferredoxin)


organism: Zea mays

substrate_name: Reduced ferredoxin; Sulfite

substrate: Reduced ferredoxin; Sulfite


organism: Eschscholzia californica

substrate_name: (R,S)-Stylopine

substrate: (R,S)-Stylopine


organism: Papaver somniferum

substrate_name: (R,S)-stylopine

proof_lines: varying concentration from 0.5 to 32 microM at a saturating concentration of 20 microM of S-adenosyl-L-methionine

proof_lines: varying concentration from 0.625 to 40 microM, at a saturating concentration of 50 microM of (R,S)-stylopine

substrate: (R,S)-stylopine


organism: Haloferax volcanii

substrate_name: L-selenocysteine

substrate: L-selenocysteine


organism: Leuconostoc mesenteroides


organism: Mus musculus


organism: Rattus norvegicus

substrate_name: Unknown

ki_unit: mM

substrate: Unknown


organism: Nocardia globerula

substrate_name: H+; NADPH; O2; Cyclohexanone

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

assay_conditions_summary: cofactor NADPH

proof_lines:
isozyme type 1
isozyme type 2

substrate: H+; NADPH; O2; Cyclohexanone


organism: Unknown

substrate_name: Thiane

substrate: Thiane


organism: Acinetobacter johnsonii

substrate_name: cyclohexanone

source_db: uniprot

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

substrate: cyclohexanone

source: uniprot


substrate_name: ferredoxin

substrate: ferredoxin


organism: Homo sapiens

substrate_name: GalNAc-alpha-Bn

substrate: GalNAc-alpha-Bn


organism: Acinetobacter calcoaceticus


proof_lines: pH 7.0, 25°C, mutant enzyme T56S/L244R/I491


proof_lines: pH 7.0, 25°C, mutant enzyme T56S/L435N/I491


organism: Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4)

substrate_name: nitrite

substrate: nitrite


organism: Escherichia coli (strain K12)

substrate_name: glutathione

proof_lines: kcat is 15, 14, 7.2 and 4.9 sec(-1) for the reduction of GS-MHQ, GS-HQ, GS-HHQ and GS-menadiol, respectively.

substrate: glutathione


substrate_name: cryptopine

proof_lines: kcat is 0.034 sec(-1) with cryptopine as substrate.

substrate: cryptopine


organism: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


organism: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)


organism: Salinispora arenicola (strain CNS-205)


organism: Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / JCM 13857 / NBRC 105044 / CNB-440)

substrate_name: (R)-S-adenosyl-L-methionine

substrate: (R)-S-adenosyl-L-methionine


organism: Streptomyces sp

substrate_name: S-adenosyl-L-methionine (for fluorinase activity)

proof_lines: kcat is 0.262 min(-1) for fluorinase activity (PubMed:24449539). kcat is 0.22 min(-1) for fluorinase activity (PubMed:27739177). kcat is 0.017 min(-1) for SAM hydrolase activity (PubMed:36996195). kcat is 0.008 min(-1) for chlorinase activi


organism: Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)


proof_lines: kcat is 30.6 sec(-1) with 1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) as substrate. kcat is 85.3 sec(-1) with 1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) as substrate with Mg(2+). kca


substrate_name: 1D-myo-inositol 1,4,5-trisphosphate

proof_lines: kcat is 6.5 sec(-1) with D-myo-inositol 1,4,5-trisphosphate.

substrate: 1D-myo-inositol 1,4,5-trisphosphate


organism: Bifidobacterium longum subsp. longum

proof_lines: kcat is 199 sec(-1) with alpha-D-Galp-(1->3)-alpha-L-Araf-OMe as substrate (PubMed:33674431). kcat is 12.1 sec(-1) with beta-L-Arap-(1->3)-alpha-L-Araf-OMe as substrate (PubMed:33674431).


organism: Enterococcus faecalis


organism: Synechocystis sp.

substrate_name: reduced ferredoxin

proof_lines: substrate Leptolyngbya boryana ferredoxin mutant E94Q/E95Q, pH 7.5, 25°C

substrate: reduced ferredoxin


proof_lines: substrate Leptolyngbya boryana ferredoxin mutant E31Q/E32Q/D36N, pH 7.5, 25°C


proof_lines: substrate Leptolyngbya boryana ferredoxin mutant D67N/D68N/D69N, pH 7.5, 25°C


proof_lines: substrate Leptolyngbya boryana ferredoxin, pH 7.5, 25°C

proof_lines: substrate maize ferredoxin, pH 7.5, 25°C


organism: Zea mays


organism: Salinispora arenicola


assay_conditions_summary: pH 7; buffer 30 mM potassium phosphate; cofactor NADP+

proof_lines: using E92Q/E30Q/D34N mutant ferredoxin, the reaction mixture contains regulator of fumarate and nitrate reduction (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM

proof_lines: using E92Q/E30Q/D34N mutant ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl


proof_lines: using E92Q/E93Q/E29Q/D34N mutant ferredoxin, the reaction mixture contains regulator of fumarate and nitrate reduction (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 2

proof_lines: using E92Q/E93Q/E29Q/D34N mutant ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl


proof_lines: using wild type ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl


assay_conditions_summary: pH 7; buffer 30-mM potassium phosphate; cofactor NADP+

proof_lines: using E92Q/E93Q mutant ferredoxin, the reaction mixture contains regulator of fumarate and nitrate reduction (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30-mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl

proof_lines: using E92Q/E93Q mutant ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl


proof_lines: pH 8.6, 25°C, recombinant and native enzyme


organism: Acinetobacter sp.

substrate_name: delta-thiovalerolactone

proof_lines: substrate inactivates enzyme after a few turnovers

substrate: delta-thiovalerolactone


organism: Exophiala jeanselmei

substrate_name: NADPH

substrate: NADPH


organism: Xanthobacter sp.


organism: Chlamydomonas reinhardtii

proof_lines: cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication

proof_lines: cosubstrate Fdx2, pH 7.5, temperature not specified in the publication


organism: Chlorolobion braunii

substrate_name: oxidized ferredoxin

substrate: oxidized ferredoxin


organism: Cyberlindnera jadinii

substrate_name: methyl viologen

substrate: methyl viologen


organism: Lactococcus cremoris

substrate_name: benzoylformate

substrate: benzoylformate


organism: Lactococcus lactis

assay_conditions_summary: pH 7; 310.15 K; cofactor NADH


proof_lines: pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2


organism: Haloferax mediterranei

substrate_name: NADH

assay_conditions_summary: pH 9; 325.15 K; buffer 0.1 M glycine-NaOH; cofactor NADH

proof_lines: with 2-oxobutyrate as cosubstrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C

proof_lines: in 0.1 M acetate buffer, pH 5.0, 4 M NaCl, at 52°C

proof_lines: with 2-oxoisocaproate as cosubstrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C

substrate: NADH


organism: Lacticaseibacillus casei

assay_conditions_summary: pH 7

proof_lines: pH 7.0, temperature not specified in the publication


organism: Lactiplantibacillus plantarum

substrate_name: phenylpyruvate

assay_conditions_summary: pH 8; 303.15 K; buffer 100 mM Tris-HC1

proof_lines: in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C

substrate: phenylpyruvate


organism: Limnospira platensis


organism: Scenedesmus fuscus


organism: Spinacia oleracea


organism: Cupriavidus necator


organism: Populus trichocarpa


organism: Saccharomyces cerevisiae


substrate_name: sulfite

proof_lines: 69K enzyme, reduced methyl viologen as electron donor

proof_lines: 63K enzyme, ferredoxin als electeron donor

substrate: sulfite


organism: Corydalis vaginans

substrate_name: (S)-Stylopine

substrate: (S)-Stylopine


organism: Papaver somniferum


organism: Sanguinaria canadensis


organism: Brassica rapa

proof_lines:
root enzyme
leaf enzyme


organism: Cyanidioschyzon merolae


organism: Methanocaldococcus jannaschii

substrate_name: reduced coenzyme F420

proof_lines: at a fixed sulfite concentration of 0.29 mM and within a reduced coenzyme F420 concentration range of 0.004-0.06 mM

proof_lines: within a sulfite concentration range of 0.0014-0.3 mM and with a fixed reduced coenzyme F420 concentration of 0.04 mM

substrate: reduced coenzyme F420


organism: Acacia verek

substrate_name: Laminarin

proof_lines:
reaction with glucose 1-phosphate
phosphorolytic reaction

substrate: Laminarin


organism: Ochromonas danica

substrate_name: laminaritriose

proof_lines:
with phosphate, pH 7.5, 30°C
with laminaritriose, pH 7.5, 30°C

substrate: laminaritriose


organism: Poterioochromonas malhamensis


organism: Thermosipho africanus

substrate_name: xylose

substrate: xylose


organism: Salinispora arenicola

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


organism: Arabidopsis thaliana

substrate_name: L-selenocysteine

substrate: L-selenocysteine


organism: Citrobacter freundii

substrate_name: beta-chloro-L-alanine

substrate: beta-chloro-L-alanine


organism: Escherichia coli


organism: Homo sapiens


enzyme_display_name: L(+)-tartrate dehydratase

substrate_name: (R,R)-Tartrate

enzyme: L(+)-tartrate dehydratase

substrate: (R,R)-Tartrate


organism: Pseudomonas putida

proof_lines:
elimination of air
in the presence of air
relatively anaerobic conditions


enzyme_display_name: dTDP-4-dehydrorhamnose 3,5-epimerase

organism: Salmonella enterica

enzyme: dTDP-4-dehydrorhamnose 3,5-epimerase


enzyme_display_name: methyl farnesoate epoxidase

organism: Locusta migratoria

substrate_name: methyl (2E,6E)-farnesoate

assay_conditions_summary: pH 8; 303.15 K; cofactor NADPH

enzyme: methyl farnesoate epoxidase

substrate: methyl (2E,6E)-farnesoate


organism: Oryctolagus cuniculus


organism: Rattus norvegicus


organism: Sus scrofa


organism: Aneurinibacillus thermoaerophilus


organism: Salmonella typhimurium


organism: Streptococcus suis


proof_lines:
pH 7.0, 25°C
native


organism: Pseudomonas fluorescens

substrate_name: H+; NADPH; O2; Cyclohexanone

substrate: H+; NADPH; O2; Cyclohexanone


organism: Amycolatopsis orientalis


organism: Streptococcus entericus


organism: Diploptera punctata

substrate_name: Methyl (2E,6E)-farnesoate; O2; [reduced NADPH-hemoprotein reductase]


substrate_name: S-Adenosyl-L-methionine; Uridine(2552) in 23S rRNA


organism: Klebsiella pneumoniae

proof_lines: mutant enzyme Q88E, pH and temperature not specified in the publication


proof_lines: wild-type enzyme, pH and temperature not specified in the publication


source_db: skid

source_license: CC BY-NC-ND 4.0

source: skid


enzyme_display_name: arsenate reductase (cytochrome c)

organism: Hydrogenophaga sp.

substrate_name: arsenite

proof_lines: pH 5.5, temperature not specified in the publication

enzyme: arsenate reductase (cytochrome c)

substrate: arsenite


organism: Pseudorhizobium banfieldiae


enzyme_display_name: hydroperoxy fatty acid reductase

organism: Synechocystis sp.

substrate_name: NADPH

assay_conditions_summary: pH 8.2; 310.15 K; cofactor NADPH

proof_lines:
isoform Gpx-1, at pH 8.2 and 37°C
isoform Gpx-2, at pH 8.2 and 37°C

enzyme: hydroperoxy fatty acid reductase

substrate: NADPH


enzyme_display_name: sulfur oxygenase/reductase

organism: Acidianus ambivalens

substrate_name: sulfur

proof_lines: pH 7.2, 65°C, reductase reaction (formation of sulfite plus thiosulfate)

proof_lines: pH 7.2, 65°C, oxygenase reaction (formation of hydrogen sulfide)

proof_lines: pH 7.2, 65°C, reductase reaction (formation of sulphite plus thiosulfate)

enzyme: sulfur oxygenase/reductase

substrate: sulfur


enzyme_display_name: glycine betaine monooxygenase

organism: Chromohalobacter israelensis

substrate_name: NADH

assay_conditions_summary: pH 7; 298.15 K; cofactor NADPH

enzyme: glycine betaine monooxygenase

substrate: NADH


enzyme_display_name: (R)-limonene 6-monooxygenase

organism: Carum carvi

substrate_name: (+)-limonene

proof_lines:
annual caraway
biannual caraway

enzyme: (R)-limonene 6-monooxygenase

substrate: (+)-limonene


enzyme_display_name: chlordecone reductase

organism: Meriones unguiculatus

enzyme: chlordecone reductase


organism: Halothiobacillus neapolitanus

substrate_name: Unknown

substrate: Unknown


substrate_name: sulfur (at 65 degrees Celsius, with the oxygenase reaction)


enzyme_display_name: trehalose O-mycolyltransferase

organism: Mycobacterium leprae

substrate_name: alpha,alpha-trehalose 6-monomycolate

proof_lines: wild-type, short-chain trehalose monomycolate is used as a substrate, Vmax: 3.83 nmol/min/nmol

enzyme: trehalose O-mycolyltransferase

substrate: alpha,alpha-trehalose 6-monomycolate


proof_lines: mutant L130S, short-chain trehalose monomycolate is used as a substrate, Vmax: 4.27 nmol/min/nmol


enzyme_display_name: ferric-chelate reductase [NAD(P)H]

organism: Archaeoglobus fulgidus

assay_conditions_summary: pH 7; 358.15 K; cofactor NADPH

proof_lines:
pH 7.0, 85°C
pH 7.0, 85°C, cosubstrate: NADPH

enzyme: ferric-chelate reductase [NAD(P)H]


enzyme_display_name: NAD(P)H sulfur oxidoreductase (CoA-dependent)

organism: Pyrococcus furiosus


organism: Thermococcus litoralis

substrate_name: polysulfide(n)

assay_conditions_summary: pH 8.9; 338.15 K; cofactor NADPH

enzyme: NAD(P)H sulfur oxidoreductase (CoA-dependent)

substrate: polysulfide(n)


organism: Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)

substrate_name: NADH (with Fe(+3) as acceptor)

substrate: NADH (with Fe(+3) as acceptor)


organism: Vibrio vulnificus


enzyme_display_name: methylarsonate reductase

organism: Caenorhabditis elegans

enzyme: methylarsonate reductase


enzyme_display_name: lipoyl(octanoyl) transferase

organism: Escherichia coli (strain K12)

substrate_name: octanoyl-ACP

enzyme: lipoyl(octanoyl) transferase

substrate: octanoyl-ACP


organism: Oryctolagus cuniculus

substrate_name: monomethylarsonate

substrate: monomethylarsonate


organism: Mycolicibacterium smegmatis


organism: Escherichia coli

substrate_name: octanoyl-[acyl-carrier protein]

substrate: octanoyl-[acyl-carrier protein]


enzyme_display_name: sphingoid base N-stearoyltransferase

organism: Homo sapiens

substrate_name: dihydrosphingosine

proof_lines: pH and temperature not specified in the publication

enzyme: sphingoid base N-stearoyltransferase

substrate: dihydrosphingosine


organism: Mus musculus

substrate_name: stearoyl-CoA

assay_conditions_summary: pH 7.4; 298.15 K; cofactor CoA

substrate: stearoyl-CoA


enzyme_display_name: zeatin O-beta-D-xylosyltransferase

organism: Phaseolus vulgaris

substrate_name: UDPxylose; Zeatin

enzyme: zeatin O-beta-D-xylosyltransferase

substrate: UDPxylose; Zeatin


organism: Sus scrofa

substrate_name: pyridylaminated lacto-N-neotetraose

substrate: pyridylaminated lacto-N-neotetraose


organism: Niallia circulans

substrate_name: dextran T-40

substrate: dextran T-40


organism: Thermoanaerobacter thermocopriae

substrate_name: Dextran

proof_lines: wild-type, K0.5 value, Hill coefficient 0.76, pH 6.0, 55°C

substrate: Dextran


proof_lines: variant lacks the long isomaltooligosaccharide-forming domain, K0.5 value, Hill coefficient 0.73 pH 6.0, 55°C

proof_lines: variant lacks both the long isomaltooligosaccharide-forming domain and the carbohydrate-binding module, K0.5 value, Hill coefficient 0.26, pH 6.0, 55°C


substrate_name: UDP-xylose

substrate: UDP-xylose


enzyme_display_name: 3-alpha-mycarosylerythronolide B desosaminyl transferase

organism: Saccharopolyspora erythraea

substrate_name: 3-alpha-mycarosylerythronolide B

proof_lines: pH and temperature not specified in the publication, 0.05 mM TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose

proof_lines: pH 8.0, 25°C, in the presence og 0.001 mM EryC and 1 mM of each substrate, more than 300 turnovers are observed in 2.5 min, indicating that the kcat must be greater than 100/min. This is one of the highest turnover numbers reported for an a

proof_lines: pH and temperature not specified in the publication, 0.05 mM dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose

substrate: 3-alpha-mycarosylerythronolide B


substrate_name: UDP-alpha-D-glucose

sequence_source: UniProtKB B5L3F2 identity anchor

proof_lines: pH 7.0, 37°C, assayed with bacterial membranes

substrate: UDP-alpha-D-glucose


sequence_source: UniProtKB Q077R2 identity anchor


organism: Saccharomyces cerevisiae

substrate_name: 2-deoxy-D-glucose 6-phosphate

proof_lines:
isoenzyme DogR1p
isoenzyme DogR2p

substrate: 2-deoxy-D-glucose 6-phosphate


enzyme_display_name: signal peptidase II

organism: Unknown

substrate_name: prolipoprotein

enzyme: signal peptidase II

substrate: prolipoprotein


enzyme_display_name: 2,4'-dihydroxyacetophenone dioxygenase

organism: Burkholderia sp.

enzyme: 2,4'-dihydroxyacetophenone dioxygenase


organism: Glycine max


enzyme_display_name: formyltetrahydrofolate deformylase

enzyme: formyltetrahydrofolate deformylase


enzyme_display_name: phenylacetaldehyde synthase

organism: Petunia x hybrida

substrate_name: L-phenylalanine

enzyme: phenylacetaldehyde synthase

substrate: L-phenylalanine


enzyme_display_name: inositol-tetrakisphosphate 5-kinase

organism: Arabidopsis thaliana

enzyme: inositol-tetrakisphosphate 5-kinase


assay_conditions_summary: cofactor ATP


organism: Rattus norvegicus


enzyme_display_name: deoxyribokinase

organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

enzyme: deoxyribokinase


enzyme_display_name: succinyl-CoA hydrolase

substrate_name: succinyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: succinyl-CoA hydrolase

substrate: succinyl-CoA


substrate_name: decanoyl-CoA

substrate: decanoyl-CoA


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

substrate_name: inhibitor of cysteine peptidase

substrate: inhibitor of cysteine peptidase


organism: Staphylococcus aureus (strain USA300)

substrate_name: P.aeruginosa inhibitor of cysteine peptidase


enzyme_display_name: N(alpha)-acyl-L-glutamine aminoacylase

organism: Corynebacterium striatum

enzyme: N(alpha)-acyl-L-glutamine aminoacylase


enzyme_display_name: glycine betaine monooxygenase

organism: Chromohalobacter israelensis (strain ATCC BAA-138 / DSM 3043 / CIP 106854 / NCIMB 13768 / 1H11)

substrate_name: NADH (for flavin reductase activity, with FAD as cosubstrate)

assay_conditions_summary: cofactor NADH

proof_lines: kcat is 0.90 sec(-1) with NADH as substrate (for flavin reductase activity, with FAD as cosubstrate). kcat is 0.35 sec(-1) with NADPH as substrate (for flavin reductase activity, with FAD as cosubstrate). kcat is 4.25 sec(-1) with FAD as su

enzyme: glycine betaine monooxygenase


substrate_name: glycine betaine

proof_lines: kcat is 0.65 sec(-1) with glycine betaine as substrate.

substrate: glycine betaine


enzyme_display_name: (R)-limonene 6-monooxygenase

organism: Thymus vulgaris

substrate_name: gamma-terpinene

proof_lines: kcat is 1.24 sec(-1) with gamma-terpinene as substrate (Ref.1). kcat is 0.08 sec(-1) with (4R)-limonene as substrate (Ref.1). kcat is 0.15 sec(-1) with (4S)-limonene as substrate (Ref.1).

enzyme: (R)-limonene 6-monooxygenase

substrate: gamma-terpinene


enzyme_display_name: chlordecone reductase

organism: Homo sapiens

proof_lines: kcat is 1.99 min(-1) for 17beta-hydroxy-5alpha-androstan-3-one reduction. kcat is 1.79 min(-1) for 5alpha-androstan-3,17-dione reduction. kcat is 2.1 min(-1) for 5alpha-androstane-3alpha,17beta-diol oxydation. kcat is 1.39.1 min(-1) for 3al

enzyme: chlordecone reductase


organism: Macaca fuscata fuscata

proof_lines: kcat is 4.9 min(-1) for 5-beta-pregnan-20-alpha-ol-3-one as substrate. kcat is 4.9 min(-1) for 3alpha-hydroxy-5alpha-androstan-17-one as substrate. kcat is 23 min(-1) for 5alpha-pregnane-3alpha,21-diol-20-one.


enzyme_display_name: NAD(P)H sulfur oxidoreductase (CoA-dependent)

organism: Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)

substrate_name: NADH

proof_lines: kcat is 68 sec(-1) for NADH oxidase activity. kcat is 50 sec(-1) for NADPH oxidase activity.

substrate: NADH


enzyme_display_name: trehalose O-mycolyltransferase

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: C26:0-CoA (with 1,2-dipalmitin as the acyl acceptor and at 37 degrees Celsius)

enzyme: trehalose O-mycolyltransferase


organism: Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)

enzyme: 3-alpha-mycarosylerythronolide B desosaminyl transferase


organism: Mus musculus

substrate_name: acetyl-CoA

proof_lines: In summary, KM for medium- to long-chain acyl CoAs is in the order 1.4-6.7 uM, and with short-chain acyl CoAs range from 8 to 30 uM. KM for bile acid-CoA esters is in the range 6-15 uM.

substrate: acetyl-CoA


enzyme_display_name: thiamine phosphate phosphatase

substrate_name: thiamine monophosphate

proof_lines: kcat is 29.9 sec(-1) for thiamine monophosphate.

enzyme: thiamine phosphate phosphatase

substrate: thiamine monophosphate


proof_lines: kcat is 13.5 sec(-1) for thiamine monophosphate.


organism: Escherichia coli (strain K12)

substrate_name: 2-deoxyglucose-6-P (with manganese ions as cofactor and at pH 9)

assay_conditions_summary: pH 9

proof_lines: kcat is 33 sec(-1) with 2-deoxyglucose-6-P as substrate. kcat is 11 sec(-1) with mannose-6-P as substrate. kcat is 9.4 sec(-1) with 2-deoxyribose-5-P as substrate. kcat is 2.8 sec(-1) with ribose-5-P as substrate. kcat is 25 sec(-1) with gl


enzyme_display_name: dolichyldiphosphatase

substrate_name: dolichyl diphosphate

enzyme: dolichyldiphosphatase

substrate: dolichyl diphosphate


organism: Burkholderia sp. AZ11


enzyme_display_name: 7-deoxycylindrospermopsin hydroxylase

organism: Oscillatoria sp. PCC 7926

proof_lines: pH and temperature not specified in the publication

enzyme: 7-deoxycylindrospermopsin hydroxylase


enzyme_display_name: (S)-3-hydroxyacid-ester dehydrogenase

organism: Klebsiella pneumoniae

substrate_name: benzyl 2-methyl-3-oxobutanoate

enzyme: (S)-3-hydroxyacid-ester dehydrogenase

substrate: benzyl 2-methyl-3-oxobutanoate


organism: Saccharomyces cerevisiae

substrate_name: ethyl 3-oxobutyrate

assay_conditions_summary: cofactor NADPH

substrate: ethyl 3-oxobutyrate


enzyme_display_name: arsenate reductase (azurin)

organism: Alcaligenes faecalis

substrate_name: azurin

enzyme: arsenate reductase (azurin)

substrate: azurin


organism: Arthrobacter sp.

substrate_name: arsenite

assay_conditions_summary: pH 6

substrate: arsenite


organism: Chrysiogenes arsenatis

substrate_name: arsenate

substrate: arsenate


enzyme_display_name: bis-gamma-glutamylcystine reductase

organism: Halobacterium salinarum

substrate_name: FAD

enzyme: bis-gamma-glutamylcystine reductase

substrate: FAD


enzyme_display_name: isoorientin 3'-O-methyltransferase

organism: Silene latifolia subsp. alba

substrate_name: caffeic acid

proof_lines: second substrate isoorientin 2-O-rhamnoside

enzyme: isoorientin 3'-O-methyltransferase

substrate: caffeic acid


organism: Malonomonas rubra

substrate_name: malonate

assay_conditions_summary: pH 6; cofactor CoA

substrate: malonate


enzyme_display_name: formaldehyde transketolase

organism: Acinetobacter sp.

substrate_name: formaldehyde

enzyme: formaldehyde transketolase

substrate: formaldehyde


organism: [Candida] boidinii

substrate_name: D-xylulose 5-phosphate

substrate: D-xylulose 5-phosphate


enzyme_display_name: noranthrone synthase

organism: Aspergillus parasiticus

substrate_name: hexanoyl-[acyl-carrier protein]

enzyme: noranthrone synthase

substrate: hexanoyl-[acyl-carrier protein]


enzyme_display_name: monoterpenol O-acetyltransferase

organism: Mentha x piperita

substrate_name: (-)-menthol

proof_lines:
leaf enzyme
flower head enzyme

enzyme: monoterpenol O-acetyltransferase

substrate: (-)-menthol


enzyme_display_name: heme oxygenase (biliverdin-IX-beta and delta-forming)

organism: Pseudomonas aeruginosa

substrate_name: heme

substrate: heme


organism: Pseudomonas fluorescens

proof_lines: PigA, pH and temperature not specified in the publication

enzyme: heme oxygenase (biliverdin-IX-beta and delta-forming)


substrate_name: bis-gamma-glutamylcystine

proof_lines: pH 6.7, temperature not specified in the publication

substrate: bis-gamma-glutamylcystine


enzyme_display_name: farnesylcysteine lyase

organism: Arabidopsis thaliana

enzyme: farnesylcysteine lyase


organism: Staphylococcus aureus

substrate_name: 2-methyladenine2503 in 23S rRNA fragment (2447-2625)


enzyme_display_name: U6 snRNA m(6)A methyltransferase

organism: Homo sapiens

substrate_name: S-adenosyl-L-methionine

proof_lines: with U6 snRNA as cosubstrate, in 10 mM HEPES, 150 mM KCl, 10 mM MgCl2, and 5 mM tris(2-carboxyethyl)phosphine, at pH 7.5 and 37°C

proof_lines: N-terminal methyltransferase domain, at pH 8.0 and 37°C

proof_lines: Km above 0.4 mM, with methionine adenosine transferase 2A mRNA hairpin 1 as cosubstrate, in 10 mM HEPES, 150 mM KCl, 10 mM MgCl2, and 5 mM tris(2-carboxyethyl)phosphine, at pH 7.5 and 37°C

enzyme: U6 snRNA m(6)A methyltransferase

substrate: S-adenosyl-L-methionine


substrate_name: recombinant nucleosome

proof_lines: cosubstrate chicken nucleosome, pH 9.0, 23°C

proof_lines: cosubstrate recombinant nucleosome, pH 9.0, 23°C

substrate: recombinant nucleosome


substrate_name: L-malate

substrate: L-malate


enzyme_display_name: chlorophyll synthase

organism: Synechocystis sp. PCC 6803

substrate_name: chlorophyllide a

proof_lines: pH 7.6, 30°C, recombinant wild-type enzyme

enzyme: chlorophyll synthase

substrate: chlorophyllide a


proof_lines: pH 7.6, 30°C, recombinant enzyme mutant I44F


organism: Pyrococcus horikoshii

substrate_name: GTP

substrate: GTP


enzyme_display_name: ceramide phosphoethanolamine synthase

organism: Mus musculus

enzyme: ceramide phosphoethanolamine synthase


enzyme_display_name: Transferred entry: 3.6.1.71

proof_lines: nicked DNA substrate, pH 7.5, 25-37°C, recombinant His-tagged wild-type enzyme

proof_lines: blunt DNA substrate, pH 7.5, 25-37°C, recombinant His-tagged wild-type enzyme

enzyme: Transferred entry: 3.6.1.71


enzyme_display_name: alpha-mannosidase

organism: Rattus norvegicus

substrate_name: H2O; p-Nitrophenyl-alpha-D-mannoside

enzyme: alpha-mannosidase

substrate: H2O; p-Nitrophenyl-alpha-D-mannoside


substrate_name: Unknown

substrate: Unknown


substrate_name: Succinyl-YVAD-p-nitroanilide; H2O

substrate: Succinyl-YVAD-p-nitroanilide; H2O


enzyme_display_name: neprilysin

proof_lines:
pH 6.5, 37°C, mutant NEPF563I
pH 6.5, 37°C, mutant NEPS546A
pH 6.5, 37°C, mutant NEPS546T

enzyme: neprilysin


substrate_name: amyloid beta peptide1-40

proof_lines: mutant G399V, pH and temperature not specified in the publication

substrate: amyloid beta peptide1-40


proof_lines: wild-type enzyme, pH and temperature not specified in the publication

proof_lines: pH 6.5, 37°C, wild-type enzyme and mutant NEPF563V


proof_lines: mutant G714K, pH and temperature not specified in the publication


proof_lines: mutant G399V/G714K, pH and temperature not specified in the publication


proof_lines: pH 7.5, 37°C, in presence of activating K49-P1-20

proof_lines: pH and temperature not specified in the publication


enzyme_display_name: leucine transaminase

organism: Glycine max

substrate_name: L-leucine

proof_lines:
isozyme LAT I, pH 9, 37°C
isozyme LAT II, pH 9, 37°C

enzyme: leucine transaminase

substrate: L-leucine


enzyme_display_name: cysteine-conjugate transaminase

proof_lines:
isoenzyme CAT-IIA, pH 7.0, 35°C
isoenzyme CAT-IIB, pH 7.0, 35°C
isoenzyme CAT-I, pH 7.0, 35°C

enzyme: cysteine-conjugate transaminase


enzyme_display_name: thermolysin

organism: Bacillus thermoproteolyticus

substrate_name: Z-Phe-Leu-Ala-OH; H2O

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

proof_lines: pH 8.0, 70°C, recombinant enzyme, autoactivation, without radiofrequency

proof_lines: pH 8.0, 70°C, recombinant enzyme, autoactivation, with radiofrequency

source_databases_merged: sabio_rk brenda skid

enzyme: thermolysin

substrate: Z-Phe-Leu-Ala-OH; H2O


proof_lines: purified recombinant mutant N116A, pH 7.5, 25°C


proof_lines: purified recombinant mutant N116D, pH 7.5, 25°C


proof_lines: purified recombinant mutant N116Q, pH 7.5, 25°C


proof_lines: purified recombinant wild-type enzyme, pH 7.5, 25°C


proof_lines: purified recombinant mutant N116T, pH 7.5, 25°C


enzyme_display_name: arabinose-5-phosphate isomerase

organism: Bacteroides fragilis

substrate_name: D-ribulose 5-phosphate

proof_lines:
pH 8.5, 37°C
Raw SKiD Env: Temp=37°C, pH=8.5

enzyme: arabinose-5-phosphate isomerase

substrate: D-ribulose 5-phosphate


organism: Clostridium tetani

substrate_name: D-arabinose 5-phosphate

substrate: D-arabinose 5-phosphate


organism: Escherichia coli


organism: Francisella tularensis

substrate_name: D-Arabinose 5-phosphate

substrate: D-Arabinose 5-phosphate


substrate_name: N-carbobenzoxy-L-Asp-L-Phe methyl ester

assay_conditions_summary: pH 7.5; 298.15 K; buffer 40 mM HEPES-NaOH

proof_lines: mutant enzyme S53D/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme S53D/G8C/N60C/S65P, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

substrate: N-carbobenzoxy-L-Asp-L-Phe methyl ester


proof_lines: mutant enzyme L155A/G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme L155A/G8C/N60C/S65P, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme L155A/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


proof_lines: mutant enzyme S53D/L155A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme S53D/L155A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme S53D/L155A, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


substrate_name: L-phenylalanine methyl ester

proof_lines: mutant enzyme D150E, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme D150E, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

substrate: L-phenylalanine methyl ester


substrate_name: N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide

proof_lines: mutant enzyme S53D, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme S53D, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme S53D, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

substrate: N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide


proof_lines: mutant enzyme S53D/L155A/G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme S53D/L155A/G8C/N60C/S65P, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme S53D/L155A/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


proof_lines: mutant enzyme L144S, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme L144S, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


proof_lines: mutant enzyme L155A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme L155A, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


proof_lines: pH 7.5, 25°C, recombinant mutants Q128K and Q225R


proof_lines: mutant enzyme D150E/I168A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


proof_lines: mutant enzyme L144S/D150E, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme L144S/D150E, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme L144S/D150E, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


proof_lines: mutant enzyme L144S/D150E/S53D, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme L144S/D150E/S53D, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme L144S/D150E/S53D, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


proof_lines: mutant enzyme I168A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme I168A, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


substrate_name: p-nitrophenyl-alpha-D-mannopyranoside (pNP-Man) (at 37 degrees Celsius and at pH 4.5)


sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


enzyme_display_name: cyanide hydratase

organism: Aspergillus niger

substrate_name: cyanide

enzyme: cyanide hydratase

substrate: cyanide


organism: Fusarium solani


proof_lines: pH 7.5, 25°C, recombinant mutants Q128E and Q225E


organism: Bacillus sp. (in: firmicutes)

substrate_name: N-benzyloxycarbonyl-Gly-L-Leu amide

proof_lines: recombinant mutant L155S/I156V, pH 7.0, 37°C

substrate: N-benzyloxycarbonyl-Gly-L-Leu amide


proof_lines: recombinant mutant L155A/I156N, pH 7.0, 37°C


proof_lines: recombinant mutant L155S/I156N, pH 7.0, 37°C


proof_lines: recombinant mutant L155A/I156V, pH 7.0, 37°C


proof_lines: recombinant wild-type enzyme, pH 7.0, 37°C


organism: Oryctolagus cuniculus


substrate_name: leucine enkephalin

substrate: leucine enkephalin


proof_lines: pH 7.0, 25°C, recombinant mutant G8C/N60C/S65P/L144S


assay_conditions_summary: pH 7; 298.15 K; buffer 40 mM HEPES-NaOH

proof_lines: pH 7.0, 25°C, recombinant mutant G8C/N60C/S65P

proof_lines: mutant enzyme G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C

proof_lines: mutant enzyme G8C/N60C/S65P, in the presence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C


enzyme_display_name: dipeptidase E

organism: Salmonella enterica subsp. enterica serovar Typhimurium

enzyme: dipeptidase E


enzyme_display_name: 2-aminobenzenesulfonate 2,3-dioxygenase

organism: Alcaligenes sp.

substrate_name: benzenesulfonate

enzyme: 2-aminobenzenesulfonate 2,3-dioxygenase

substrate: benzenesulfonate


enzyme_display_name: aklavinone 12-hydroxylase

organism: Streptomyces purpurascens

substrate_name: NADPH

proof_lines: pH and temperature not specified in the publication

enzyme: aklavinone 12-hydroxylase

substrate: NADPH


enzyme_display_name: ferruginol synthase

organism: Salvia rosmarinus

enzyme: ferruginol synthase


enzyme_display_name: dimethylglycine N-methyltransferase

organism: Aphanothece halophytica

substrate_name: S-adenosyl-L-methionine

enzyme: dimethylglycine N-methyltransferase

substrate: S-adenosyl-L-methionine


organism: Methanosarcina acetivorans

substrate_name: methylcob(III)alamin

proof_lines: at pH 7.2, temperature not specified in the publication

substrate: methylcob(III)alamin


enzyme_display_name: anthranilate N-benzoyltransferase

organism: Dianthus caryophyllus

substrate_name: benzoyl-CoA

enzyme: anthranilate N-benzoyltransferase

substrate: benzoyl-CoA


substrate_name: NAD (at pH 7.5)

substrate: NAD (at pH 7.5)


organism: Aspergillus parasiticus (strain ATCC 56775 / NRRL 5862 / SRRC 143 / SU-1)

substrate_name: demethylsterigmatocystin

substrate: demethylsterigmatocystin


enzyme_display_name: anthranilate N-methyltransferase

organism: Ruta graveolens

substrate_name: anthranilate

enzyme: anthranilate N-methyltransferase

substrate: anthranilate


organism: Parasynechococcus marenigrum (strain WH8102)

substrate_name: dimethylglycine (at pH 8.5 and at 37 degrees Celsius)


enzyme_display_name: acetyl-S-ACP:malonate ACP transferase

organism: Malonomonas rubra

substrate_name: malonate

enzyme: acetyl-S-ACP:malonate ACP transferase

substrate: malonate


organism: Petrotoga mobilis (strain DSM 10674 / SJ95)

substrate_name: glucosyl-3-phosphoglycerate (at 60 degrees Celsius)


enzyme_display_name: crocetin glucosyltransferase

organism: Gardenia jasminoides

substrate_name: crocetin

enzyme: crocetin glucosyltransferase

substrate: crocetin


organism: Acinetobacter calcoaceticus

substrate_name: malonyl-CoA

substrate: malonyl-CoA


organism: Klebsiella pneumoniae

assay_conditions_summary: pH 7; cofactor CoA


organism: Pseudomonas aeruginosa

proof_lines: in 50 mM HEPES (pH 8.0), 2.5 mM dithiothreitol, and 2 mM MgCl2, at 30°C


organism: Pseudomonas aeruginosa PAO1


organism: Candida maltosa

substrate_name: L-lysine

assay_conditions_summary: pH 8.1; 310.15 K; cofactor CoA

substrate: L-lysine


organism: Saccharomyces cerevisiae

substrate_name: L-thialysine

assay_conditions_summary: pH 8.5; 303.15 K; cofactor CoA

substrate: L-thialysine


enzyme_display_name: phenylpropanoylacetyl-CoA synthase

organism: Curcuma longa

substrate_name: feruloyl-CoA

assay_conditions_summary: pH 7.5; 310.15 K; cofactor CoA

enzyme: phenylpropanoylacetyl-CoA synthase

substrate: feruloyl-CoA


organism: Raphanus sativus


enzyme_display_name: isovitexin beta-glucosyltransferase

organism: Silene latifolia subsp. alba

substrate_name: UDPglucose

proof_lines:
reaction with isovitexin
reaction with isoorientin

enzyme: isovitexin beta-glucosyltransferase

substrate: UDPglucose


organism: Homo sapiens

substrate_name: UDP-galactose

substrate: UDP-galactose


organism: Notamacropus eugenii

substrate_name: lactose

substrate: lactose


enzyme_display_name: lipid-A-disaccharide synthase

organism: Escherichia coli

enzyme: lipid-A-disaccharide synthase


organism: Aspergillus niger

substrate_name: maltotriose

substrate: maltotriose


organism: Mycolicibacterium smegmatis

substrate_name: methyl-alpha-D-mannoside

substrate: methyl-alpha-D-mannoside


organism: Crocus sativus


enzyme_display_name: alpha-(1->3) branching sucrase

organism: Apilactobacillus kunkeei

substrate_name: sucrose

enzyme: alpha-(1->3) branching sucrase

substrate: sucrose


organism: Leuconostoc citreum

proof_lines:
no cosubstrate, pH 5.8, 30°C
cosubstrate dextran, pH 5.8, 30°C
pH 5.8, 30°C


enzyme_display_name: glucomannan 4-beta-mannosyltransferase

organism: Lathyrus oleraceus

substrate_name: GDP-D-mannose

enzyme: glucomannan 4-beta-mannosyltransferase

substrate: GDP-D-mannose


organism: Pinus sylvestris


organism: Vigna radiata var. radiata


organism: Aspergillus fumigatus

substrate_name: dimethylallyl diphosphate

substrate: dimethylallyl diphosphate


enzyme_display_name: leucine transaminase

organism: Rattus norvegicus

enzyme: leucine transaminase


enzyme_display_name: chlorophyll synthase

organism: Synechocystis sp. PCC 6803

substrate_name: geranylgeranyl diphosphate

assay_conditions_summary: pH 7.6; 303.15 K; buffer 120 mM potassium acetate; cofactor ATP

proof_lines: lysate mixed into reaction buffer (120 mM potassium acetate, 10 mM magnesium acetate, 50 mM HEPES-KOH, pH 7.6, 14 mM 2-mercaptoethanol, 10% glycerol, and 0.5 mM ATP), 30°C

proof_lines: in the presence of sufficient geranylgeranyl diphosphate as substrate, lysate mixed into reaction buffer (120 mM potassium acetate, 10 mM magnesium acetate, 50 mM HEPES-KOH, pH 7.6, 14 mM 2-mercaptoethanol, 10% glycerol, and 0.5 mM ATP), 30

enzyme: chlorophyll synthase

substrate: geranylgeranyl diphosphate


enzyme_display_name: AMP--thymidine kinase

organism: Asplenium nidus

substrate_name: AMP

enzyme: AMP--thymidine kinase

substrate: AMP


organism: Herpes simplex virus

substrate_name: thymidine

substrate: thymidine


enzyme_display_name: deoxyribokinase

organism: Salmonella enterica subsp. enterica serovar Typhi

enzyme: deoxyribokinase


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: ATP

assay_conditions_summary: pH 7.5; cofactor ATP

proof_lines: pH 7.5, temperature not specified in the publication

substrate: ATP


organism: Saccharomyces cerevisiae

substrate_name: phosphate

substrate: phosphate


enzyme_display_name: erythritol kinase (D-erythritol 4-phosphate-forming)

organism: Acidipropionibacterium acidipropionici

assay_conditions_summary: pH 8.6; 301.15 K; cofactor ATP


organism: Brucella abortus

assay_conditions_summary: pH 8; 298.15 K; cofactor ATP

proof_lines: pH 8.0, 25°C, recombinant erythritol kinase

enzyme: erythritol kinase (D-erythritol 4-phosphate-forming)


proof_lines:
pH 7.8, 37°C, submandibular gland
pH 7.8, 37°C, parotid gland
pH 7.8, 37°C


organism: Gallus gallus

substrate_name: UDP-N-acetylgalactosamine 4-sulfate

substrate: UDP-N-acetylgalactosamine 4-sulfate


organism: Homo sapiens

substrate_name: dermatan

proof_lines: pH 6, 37°C, Km value depends on number of disaccharides of the receptor

substrate: dermatan


enzyme_display_name: tropinesterase

organism: Oryctolagus cuniculus

substrate_name: atropine

proof_lines:
enzyme form Ef IX
enzyme form Ef VII

enzyme: tropinesterase

substrate: atropine


enzyme_display_name: succinyl-CoA hydrolase

organism: Mus musculus

substrate_name: succinyl-CoA

enzyme: succinyl-CoA hydrolase

substrate: succinyl-CoA


enzyme_display_name: thiamine phosphate phosphatase

substrate_name: thiamine phosphate

proof_lines: membrane-bound enzyme form, at pH 9.0, temperature not specified in the publication

proof_lines: soluble enzyme form, at pH 6.0, temperature not specified in the publication

enzyme: thiamine phosphate phosphatase

substrate: thiamine phosphate


organism: Zea mays

assay_conditions_summary: pH 5

proof_lines: at pH 5.0, temperature not specified in the publication


organism: Cajanus indicus

substrate_name: p-nitrophenyl beta-L-arabinoside

substrate: p-nitrophenyl beta-L-arabinoside


organism: Caldanaerobius polysaccharolyticus

substrate_name: 4-nitrophenyl beta-L-arabinopyranoside

substrate: 4-nitrophenyl beta-L-arabinopyranoside


enzyme_display_name: 2-phosphosulfolactate phosphatase

organism: Methanocaldococcus jannaschii

substrate_name: rac-phosphosulfolactate

enzyme: 2-phosphosulfolactate phosphatase

substrate: rac-phosphosulfolactate


enzyme_display_name: alanine carboxypeptidase

organism: Soil bacterium

substrate_name: N-benzoyl-L-aminobutyric acid

enzyme: alanine carboxypeptidase

substrate: N-benzoyl-L-aminobutyric acid


enzyme_display_name: dipeptidase E

enzyme: dipeptidase E


substrate_name: succinyl-casein

substrate: succinyl-casein


organism: Rattus norvegicus

substrate_name: Unknown

substrate: Unknown


enzyme_display_name: signal peptidase II

organism: Escherichia coli

substrate_name: prolipoprotein

enzyme: signal peptidase II

substrate: prolipoprotein


organism: ancestral Carnivora reconstruction


organism: Canis lupus familiaris

substrate_name: LSDGIDE

proof_lines: pH 7.2, 37°C, recombinant enzyme, calculated by BRENDA

substrate: LSDGIDE


proof_lines:
pH 7.5
pH 7.5, 37°C
at pH 7.0 and pH 7.5


enzyme_display_name: neprilysin

organism: Bos taurus

enzyme: neprilysin


proof_lines:
enzyme from glomeruli
enzyme from tubules


substrate_name: angiotensin II

proof_lines:
37°C, pH 7.4
unsulfated
sulfated

substrate: angiotensin II


organism: Lactococcus lactis

substrate_name: Met-enkephalin amide

substrate: Met-enkephalin amide


enzyme_display_name: thermolysin

organism: Bacillus sp. (in: firmicutes)

proof_lines: pH 7.0, 37°C, Co2+-bound enzyme, in presence of 1 M NaSCN

enzyme: thermolysin


organism: Bacillus thermoproteolyticus

substrate_name: N-carbobenzoxy-L-Asp-L-Phe methyl ester

proof_lines: pH 7.5, 25°C, recombinant wild-type enzyme

proof_lines: pH 7.0, 25°C, recombinant wild-type enzyme

substrate: N-carbobenzoxy-L-Asp-L-Phe methyl ester


substrate_name: GFSA

proof_lines:
pH 8.0, 37°C, 5 M glycerol, product GF
pH 7.5, 25°C, recombinant enzyme
pH 8.0, 37°C, product GF

substrate: GFSA


substrate_name: bradykinin

substrate: bradykinin


substrate_name: dansyl-Gly-Trp-Gly

substrate: dansyl-Gly-Trp-Gly


organism: Sus scrofa

substrate_name: Substance P

substrate: Substance P


enzyme_display_name: N(alpha)-acyl-L-glutamine aminoacylase

organism: Corynebacterium striatum

substrate_name: benzyloxycarbonyl-L-glutamine

enzyme: N(alpha)-acyl-L-glutamine aminoacylase

substrate: benzyloxycarbonyl-L-glutamine


enzyme_display_name: acylagmatine amidase

organism: Fusarium anguioides

substrate_name: propionyl agmatine

enzyme: acylagmatine amidase

substrate: propionyl agmatine


enzyme_display_name: amidinoaspartase

organism: Pseudomonas chlororaphis

substrate_name: N-amidino-L-aspartate

assay_conditions_summary: pH 8; buffer HEPES buffer

proof_lines:
HEPES buffer, pH 8, assay method B
phosphate buffer
HEPES buffer, pH 8, assay method A

enzyme: amidinoaspartase

substrate: N-amidino-L-aspartate


organism: Pseudomonas putida

proof_lines:
C-32 , activation with Co2+
C-32, activation with Mn2+
C-32


enzyme_display_name: guanidinoacetase

organism: Corynebacterium sp.

substrate_name: Guanidinoacetate

enzyme: guanidinoacetase

substrate: Guanidinoacetate


organism: Flavobacterium sp.


organism: Pseudomonas sp.


enzyme_display_name: salsolinol synthase

organism: Homo sapiens

substrate_name: acetaldehyde

proof_lines: with formaldehyde as cosubstrate, at pH 7.4 and 37°C

proof_lines: with acetaldehyde as cosubstrate, at pH 7.4 and 37°C

enzyme: salsolinol synthase

substrate: acetaldehyde


enzyme_display_name: dolichyldiphosphatase

substrate_name: dolichyl diphosphate

enzyme: dolichyldiphosphatase

substrate: dolichyl diphosphate


organism: Rattus norvegicus

proof_lines:
day 22 of embryonic development
day 4 of embryonic development


organism: Saccharomyces cerevisiae

proof_lines: pH 7.2, 30°C, using microsomal fractions from Sf-9 cells expressing the enzyme


enzyme_display_name: phenylacetaldehyde synthase

organism: Petunia x hybrida

substrate_name: L-phenylalanine

proof_lines: pH and temperature not specified in the publication

enzyme: phenylacetaldehyde synthase

substrate: L-phenylalanine


enzyme_display_name: trimethylamine-oxide aldolase

organism: Aminobacter aminovorans

substrate_name: Benzyldimethylamine N-oxide

enzyme: trimethylamine-oxide aldolase

substrate: Benzyldimethylamine N-oxide


substrate_name: Trimethylamine N-oxide

substrate: Trimethylamine N-oxide


organism: Dosidicus gigas

proof_lines: in 100 mM Tris-acetate (pH 7.0), 2 mM cysteine, 2 mM ascorbate, and 0.2 mM FeCl2, at 50°C


organism: Urophycis chuss

assay_conditions_summary: cofactor NADH

proof_lines: catalyzed aerobically in presence of Fe2+ (0.2 mM), ascorbate (2 mM), and cysteine (2 mM)

proof_lines: catalyzed anaerobically in presence of FMN (0.1 mM) and NADH (0.4 mM)


enzyme_display_name: 3-hydroxybutyryl-CoA dehydratase

substrate_name: Unknown

enzyme: 3-hydroxybutyryl-CoA dehydratase

substrate: Unknown


organism: Rhodospirillum rubrum

substrate_name: crotonyl-[acyl-carrier protein]

assay_conditions_summary: cofactor CoA

substrate: crotonyl-[acyl-carrier protein]


enzyme_display_name: arabinose-5-phosphate isomerase

organism: Acidipropionibacterium acidipropionici

substrate_name: D-arabinose 5-phosphate

enzyme: arabinose-5-phosphate isomerase

substrate: D-arabinose 5-phosphate


organism: Arabidopsis thaliana


organism: Escherichia coli


organism: Francisella tularensis

proof_lines: at 37°C, pH not specified in the publication


enzyme_display_name: cyanide hydratase

organism: Aspergillus niger

enzyme: cyanide hydratase


organism: Fusarium lateritium

substrate_name: CN-

substrate: CN-


organism: Stemphylium loti


enzyme_display_name: inositol 3-methyltransferase

organism: Vinca minor

substrate_name: S-Adenosyl-L-methionine; myo-Inositol

enzyme: inositol 3-methyltransferase

substrate: S-Adenosyl-L-methionine; myo-Inositol


organism: Hordeum vulgare

substrate_name: UTP; D-Glucuronate 1-phosphate

substrate: UTP; D-Glucuronate 1-phosphate


organism: Homo sapiens

substrate_name: H2O; Ac-Leu-Leu-Tyr-AFC

proof_lines: IC50 above 0.01 mM, pH and temperature not specified in the publication

substrate: H2O; Ac-Leu-Leu-Tyr-AFC


organism: Lactococcus lactis subsp. lactis


enzyme_display_name: formyltetrahydrofolate deformylase

organism: Sus scrofa

substrate_name: H2O; 10-Formyltetrahydrofolate

enzyme: formyltetrahydrofolate deformylase

substrate: H2O; 10-Formyltetrahydrofolate


enzyme_display_name: verruculogen prenyltransferase

organism: Aspergillus fischeri

substrate_name: fumitremorgin B

enzyme: verruculogen prenyltransferase

substrate: fumitremorgin B


organism: Petroselinum crispum

substrate_name: dimethylallyl diphosphate

proof_lines:
product osthenol, pH 8.0, 25°C
product demethylsuberosin, pH 8.0, 25°C

substrate: dimethylallyl diphosphate


organism: Methanothermobacter marburgensis

substrate_name: sn-Glyceryl phosphate; Geranylgeranyl diphosphate


organism: Methanothermobacter thermautotrophicus

substrate: sn-Glyceryl phosphate; Geranylgeranyl diphosphate


organism: Thermoplasma acidophilum


enzyme_display_name: macrolide 2'-kinase

substrate_name: ATP

enzyme: macrolide 2'-kinase

substrate: ATP


proof_lines:
pH 7.5, 37°C
Raw SKiD Env: Temp=37°C, pH=7.5


enzyme_display_name: keratan sulfotransferase

substrate_name: 3'-Phosphoadenylyl sulfate; Keratan sulfate

enzyme: keratan sulfotransferase


enzyme_display_name: L-threo-3-deoxy-hexylosonate aldolase

organism: Trichoderma reesei

enzyme: L-threo-3-deoxy-hexylosonate aldolase


enzyme_display_name: aklavinone 12-hydroxylase

organism: Streptomyces peucetius

substrate_name: flavokermesic acid

proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication

enzyme: aklavinone 12-hydroxylase

substrate: flavokermesic acid


proof_lines: mutant P217K, pH not specified in the publication, temperature not specified in the publication


enzyme_display_name: ferruginol synthase

organism: Salvia miltiorrhiza

proof_lines:
pH 7.5, 28°C
Raw SKiD Env: Temp=28°C, pH=7.5

enzyme: ferruginol synthase


enzyme_display_name: anthranilate N-methyltransferase

organism: Ruta graveolens

substrate_name: S-adenosyl-L-methionine

enzyme: anthranilate N-methyltransferase

substrate: S-adenosyl-L-methionine


enzyme_display_name: dimethylglycine N-methyltransferase

organism: Parasynechococcus marenigrum WH 8102

enzyme: dimethylglycine N-methyltransferase


organism: Saccharomyces cerevisiae

proof_lines: 37°C, pH not specified in the publication, cloned enzyme

proof_lines: 37°C, pH not specified in the publication, native enzyme

proof_lines: 37°C, pH not specified in the publication, native and cloned enzyme


organism: Methanosarcina barkeri

substrate_name: methanol

proof_lines:
beta subunit MtaB
Raw SKiD Env: Temp=-----, pH=-----

substrate: methanol


enzyme_display_name: lipid-A-disaccharide synthase

organism: Escherichia coli

enzyme: lipid-A-disaccharide synthase


organism: Petrotoga mobilis

substrate_name: GDP-mannose

substrate: GDP-mannose


organism: Rhodopirellula baltica


organism: Homo sapiens

substrate_name: nicotinate

assay_conditions_summary: pH 5; 295.15 K; cofactor ADP

substrate: nicotinate


organism: Oryctolagus cuniculus

substrate_name: NGDP+

assay_conditions_summary: pH 7.4; cofactor ADP

proof_lines: pH 7.4, temperature not specified in the publication

substrate: NGDP+


enzyme_display_name: erythromycin 12 hydroxylase

organism: Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)

substrate_name: erythromycin D

enzyme: erythromycin 12 hydroxylase

substrate: erythromycin D


organism: Sinomonas cyclohexanicum

proof_lines: kcat is 55 sec(-1) with trans-4-hydroxyCHCA as substrate. kcat is 0.026 sec(-1) with cis-4-hydroxyCHCA as substrate. kcat is 650 sec(-1) with 4-oxoCHCA as substrate.


organism: Amycolatopsis mediterranei (strain S699)

substrate_name: 27-O-demethylrifamycin SV

substrate: 27-O-demethylrifamycin SV


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)


enzyme_display_name: phenylpropanoylacetyl-CoA synthase

organism: Curcuma longa

substrate_name: malonyl-CoA

enzyme: phenylpropanoylacetyl-CoA synthase

substrate: malonyl-CoA


enzyme_display_name: crocetin glucosyltransferase

organism: Crocus sativus

substrate_name: crocetin

proof_lines: These parameters were determined on purified activity containing a mix of 2 enzymes, probably GLT2 and GLT3.

enzyme: crocetin glucosyltransferase

substrate: crocetin


enzyme_display_name: starch synthase (maltosyl-transferring)

organism: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

substrate_name: maltose 1-phosphate

proof_lines: The data are not very reliable because measures were done with a partially purified enzyme.

enzyme: starch synthase (maltosyl-transferring)

substrate: maltose 1-phosphate


organism: Aspergillus fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181)


enzyme_display_name: adenosylcobinamide kinase

organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

substrate_name: adenosylcobinamide

assay_conditions_summary: cofactor ATP

proof_lines: kcat is 15.1 min(-1) for adenosylcobinamide kinase activity. kcat is 5.6 min(-1) for guanylyltransferase activity.

enzyme: adenosylcobinamide kinase

substrate: adenosylcobinamide


organism: Mycolicibacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 / 3849)

substrate_name: MMP (at 37 degrees Celsius)

proof_lines: kcat is 0.011 sec(-1) (at 37 degrees Celsius). kcat is 0.021 sec(-1) (at 45 degrees Celsius).

substrate: MMP (at 37 degrees Celsius)


enzyme_display_name: funoran endo-beta-hydrolase

organism: Wenyingzhuangia aestuarii

substrate_name: funoran

proof_lines: kcat is 3.95 sec(-1) with funoran as substrate (PubMed:37479453). kcat is 176.74 sec(-1) with agarose as substrate (PubMed:32578425).

enzyme: funoran endo-beta-hydrolase

substrate: funoran


organism: Unknown

substrate_name: beta-Alanine

substrate: beta-Alanine


organism: Rattus norvegicus

substrate_name: pyruvate

substrate: pyruvate


enzyme_display_name: quercetin-3-sulfate 4'-sulfotransferase

enzyme: quercetin-3-sulfate 4'-sulfotransferase


enzyme_display_name: (deoxy)adenylate kinase

organism: Mus musculus

substrate_name: ATP

enzyme: (deoxy)adenylate kinase

substrate: ATP


enzyme_display_name: 2-phosphoglycerate kinase

organism: Methanothermus fervidus

assay_conditions_summary: pH 7.5; 348.15 K; cofactor ATP

enzyme: 2-phosphoglycerate kinase


organism: Aeropyrum pernix

substrate_name: CTP

proof_lines:
in presence of Mg2+, pH 7.5, 37°C
in absence of Mg2+, pH 7.5, 37°C

substrate: CTP


organism: Archaeoglobus fulgidus


organism: Candida albicans

substrate_name: CDP-diacylglycerol

substrate: CDP-diacylglycerol


organism: Homo sapiens

substrate_name: D-myo-Inositol 3,4-bisphosphate

substrate: D-myo-Inositol 3,4-bisphosphate


substrate_name: D-myo-Inositol 1,3,4-trisphosphate

substrate: D-myo-Inositol 1,3,4-trisphosphate


enzyme_display_name: kanosamine-6-phosphate phosphatase

organism: Bacillus cereus

substrate_name: kanosamine 6-phosphate

proof_lines: pH and temperature not specified in the publication

enzyme: kanosamine-6-phosphate phosphatase

substrate: kanosamine 6-phosphate


organism: Bacillus subtilis

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Corynebacterium striatum

assay_conditions_summary: pH 7

proof_lines: pH 7.0, temperature not specified in the publication


enzyme_display_name: cyanophycinase

organism: Synechocystis sp. PCC 6803

proof_lines: 30°C in 50 mM N-[2-hydroxy-1,1-bis(hydroxymethyl) ethyl]glycine, pH 8.0, maximum at pH 9.0

proof_lines: 30°C in 50 mM N-[2-hydroxy-1,1-bis(hydroxymethyl) ethyl]glycine, maximum at pH 8.0

enzyme: cyanophycinase


organism: Ectopseudomonas oleovorans


organism: Flaveria chlorifolia


organism: Escherichia coli

substrate_name: L-Ser

substrate: L-Ser


substrate_name: ATP; dAMP

substrate: ATP; dAMP


enzyme_display_name: acetoacetyl-CoA hydrolase

substrate_name: acetoacetyl-CoA

proof_lines:
liver cytosol, absence of glutathione
liver cytosol, presence of glutathione
brain mitochondria

enzyme: acetoacetyl-CoA hydrolase

substrate: acetoacetyl-CoA


organism: Comamonas testosteroni


enzyme_display_name: rifamycin-B oxidase

organism: Curvularia lunata

substrate_name: Rifamycin B

enzyme: rifamycin-B oxidase

substrate: Rifamycin B


organism: Humicola spp.

proof_lines:
free acetone-defatted cells
immobilized acetone-defatted cells


enzyme_display_name: benzylsuccinate synthase

organism: Thauera aromatica

substrate_name: Toluene

proof_lines:
cell extract, 4°C
cell extract, 30°C

enzyme: benzylsuccinate synthase

substrate: Toluene


organism: Geobacter metallireducens

assay_conditions_summary: pH 7; 303.15 K; buffer 50 mM potassium phosphate; cofactor CoA

proof_lines: in 50 mM potassium phosphate buffer (pH 7.0), at 30°C


organism: Syntrophus aciditrophicus


organism: Drosophila melanogaster

substrate_name: L-Dopa

proof_lines: wild type enzyme, pH and temperature not specified in the publication

substrate: L-Dopa


proof_lines: mutant enzyme N192H, pH and temperature not specified in the publication


organism: Streptomyces griseus

substrate_name: glycerone phosphate

substrate: glycerone phosphate


organism: Cereibacter sphaeroides


organism: Sus scrofa

proof_lines: Y332F DDC mutant, reaction in 50 mM Hepes, pH 7.5, at 25°C causes the production of ammonia and 3,4-dihydroxyphenylacetaldehyde along with the consumption of molecular oxygen in a 1:2 molar ratio


enzyme_display_name: 1,8-cineole 2-endo-monooxygenase

organism: Citrobacter braakii

substrate_name: [reduced NADPH-hemoprotein reductase]

enzyme: 1,8-cineole 2-endo-monooxygenase

substrate: [reduced NADPH-hemoprotein reductase]


organism: Pseudomonas sp. YGJ3


organism: Oryza sativa Japonica Group

substrate_name: myo-inositol

proof_lines: recombinant enzyme from Escherichia coli, at pH 7.4 and 30°C

proof_lines: recombinant enzyme from Saccharomyces cerevisiae, at pH 7.4 and 30°C

substrate: myo-inositol


enzyme_display_name: beta-carotene 3-hydroxylase

organism: Agrobacterium aurantiacum

substrate_name: echinenone

enzyme: beta-carotene 3-hydroxylase

substrate: echinenone


enzyme_display_name: acyl-lipid omega-6 desaturase (cytochrome b5)

organism: Carthamus tinctorius


organism: Yarrowia lipolytica

enzyme: acyl-lipid omega-6 desaturase (cytochrome b5)


enzyme_display_name: hydroxylamine oxidase (cytochrome)

organism: Paracoccus denitrificans

substrate_name: hydroxylamine

proof_lines: pH 8, temperature not specified in the publication

enzyme: hydroxylamine oxidase (cytochrome)

substrate: hydroxylamine


organism: Pseudomonas sp.

substrate_name: horse heart ferricytochrome c

substrate: horse heart ferricytochrome c


enzyme_display_name: CoA-glutathione reductase

organism: Rattus norvegicus

substrate_name: CoA-glutathione

assay_conditions_summary: pH 5.75; cofactor CoA

proof_lines: at pH 5.75 and a fixed value of 110 nmol NADPH

enzyme: CoA-glutathione reductase

substrate: CoA-glutathione


organism: Saccharomyces cerevisiae

assay_conditions_summary: pH 5.5; cofactor NADPH

proof_lines: at pH 5.5 and a fixed value of 110 nmol NADPH


organism: Staphylococcus aureus

substrate_name: CoA disulfide

assay_conditions_summary: cofactor NADPH

substrate: CoA disulfide


enzyme_display_name: thiocyanate desulfurase

organism: Thioalkalivibrio paradoxus

substrate_name: ferricytochrome c 550

enzyme: thiocyanate desulfurase

substrate: ferricytochrome c 550


organism: Thioalkalivibrio sp.

substrate_name: Unknown

substrate: Unknown


enzyme_display_name: starch synthase (maltosyl-transferring)

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: maltohexaose (in the presence of 5 mM M1P)

enzyme: starch synthase (maltosyl-transferring)


organism: Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

substrate: maltohexaose (in the presence of 5 mM M1P)


organism: Aplysia californica

substrate_name: cADPR (hydrolysis of cADPR)

substrate: cADPR (hydrolysis of cADPR)


organism: Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)

substrate_name: fumigaclavine A

substrate: fumigaclavine A


organism: Petroselinum crispum

substrate_name: umbelliferone in the reaction producing demethylsuberosin


substrate_name: D-beta-aminoisobutyrate

substrate: D-beta-aminoisobutyrate


organism: Methanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S)


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: CDP-diacylglycerol

substrate: CDP-diacylglycerol


organism: Flaveria chlorifolia

substrate_name: quercetin 3-sulfate

substrate: quercetin 3-sulfate


substrate_name: inositol 3,4-bisphosphate

substrate: inositol 3,4-bisphosphate


organism: Cellvibrio japonicus (strain Ueda107)

substrate_name: 2,4-dinitrophenyl-beta-galactobioside (at pH 7 and 37 degrees Celsius)


organism: Methanosarcina acetivorans

substrate_name: CoM-S-S-CoB

proof_lines: subunit HdrB2C2, at 21°C, pH not specified in the publication

proof_lines: subunit HdrB2, at 21°C, pH not specified in the publication

substrate: CoM-S-S-CoB


enzyme_display_name: tryptophan 2-C-methyltransferase

organism: Streptomyces laurentii

substrate_name: S-adenosyl-L-methionine

enzyme: tryptophan 2-C-methyltransferase

substrate: S-adenosyl-L-methionine


organism: Zea mays


organism: Cinchona succirubra

substrate_name: emodin

proof_lines:
isoform I
isoform III
isoform V

substrate: emodin


organism: Neomoorella thermoacetica

substrate_name: tetrahydrosarcinapterin

substrate: tetrahydrosarcinapterin


organism: Corynebacterium striatum


organism: Rattus norvegicus


organism: Pseudomonas anguilliseptica

substrate_name: cyanophycin granule polypeptide (CGP)

substrate: cyanophycin granule polypeptide (CGP)


organism: Comamonas testosteroni


organism: Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15)


organism: Syntrophus aciditrophicus (strain SB)


organism: Arabidopsis thaliana

substrate_name: L-phenylalanine

substrate: L-phenylalanine


organism: Petroselinum crispum

substrate_name: L-tyrosine

substrate: L-tyrosine


organism: Streptomyces griseus

substrate_name: L-aspartate-4-semialdehyde (at 30 degrees Celsius)


enzyme_display_name: N-acylsphingosine galactosyltransferase

organism: Gallus gallus

substrate_name: UDPgalactose

enzyme: N-acylsphingosine galactosyltransferase

substrate: UDPgalactose


proof_lines: Km value for ceramide is influenced by its dispersion state, in presence of 2 mM Triton S-100 biphasic kinetics are observed with increasing ceramide concentration, yielding two apparent Km values of 0.018 and 0.15 mM


enzyme_display_name: histidine transaminase

enzyme: histidine transaminase


organism: Streptomyces tendae

substrate_name: pyruvate

substrate: pyruvate


enzyme_display_name: guanosine phosphorylase

organism: Halorubrum lacusprofundi

substrate_name: phosphate

enzyme: guanosine phosphorylase

substrate: phosphate


organism: Oryctolagus cuniculus

substrate_name: deoxyguanosine

substrate: deoxyguanosine


enzyme_display_name: 2-amino-4-deoxychorismate synthase

organism: Burkholderia lata

substrate_name: chorismate

enzyme: 2-amino-4-deoxychorismate synthase

substrate: chorismate


enzyme_display_name: thiol sulfotransferase

organism: Cyanophora paradoxa

enzyme: thiol sulfotransferase


organism: Synechococcus sp.

assay_conditions_summary: pH 8


enzyme_display_name: 11-cis-retinyl-palmitate hydrolase

organism: Bos taurus

substrate_name: 11-cis-retinyl palmitate

enzyme: 11-cis-retinyl-palmitate hydrolase

substrate: 11-cis-retinyl palmitate


proof_lines:
enzyme from retinal pigment epithelium
enzyme from retina


organism: Homo sapiens


organism: Escherichia coli

substrate_name: acyl-carrier protein

substrate: acyl-carrier protein


organism: Pseudomonas aeruginosa

substrate_name: bis-4-nitrophenyl phosphate

substrate: bis-4-nitrophenyl phosphate


enzyme_display_name: CMP-N-acylneuraminate phosphodiesterase

substrate_name: CMP-N-acylneuraminate

enzyme: CMP-N-acylneuraminate phosphodiesterase

substrate: CMP-N-acylneuraminate


organism: Neisseria meningitidis


organism: Rattus norvegicus


enzyme_display_name: atrolysin B

organism: Crotalus atrox

substrate_name: oxidized insulin B-chain

proof_lines:
Gly23-Phe24 bond
His5-Leu6 bond
His10-Leu11 bond

enzyme: atrolysin B

substrate: oxidized insulin B-chain


enzyme_display_name: cerevisin

organism: Debaryomyces hansenii

enzyme: cerevisin


organism: Saccharomyces cerevisiae

substrate_name: benzoyl-Tyr ethyl ester

substrate: benzoyl-Tyr ethyl ester


enzyme_display_name: atrolysin E

substrate_name: dimethylcasein

proof_lines:
cobaltous toxin
Tyr14-Gln15 bond
Ala8-Ser9 bond

enzyme: atrolysin E

substrate: dimethylcasein


enzyme_display_name: trimerelysin I

organism: Protobothrops flavoviridis

enzyme: trimerelysin I


enzyme_display_name: N-acyl-D-aspartate deacylase

organism: Achromobacter xylosoxidans

substrate_name: N-Formyl-D-Asp

enzyme: N-acyl-D-aspartate deacylase

substrate: N-Formyl-D-Asp


enzyme_display_name: imidazolonepropionase

organism: Pseudomonas fluorescens

substrate_name: 4(5)-imidazolone-5(4)-propionic acid

enzyme: imidazolonepropionase

substrate: 4(5)-imidazolone-5(4)-propionic acid


organism: Salmonella enterica subsp. enterica serovar Typhimurium


enzyme_display_name: [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine

organism: Mycobacterium tuberculosis

substrate_name: O-phospho-L-serine

substrate: O-phospho-L-serine


enzyme: [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine


mutation_type: deletion

proof_lines: deletion mutant DELTA319-323, at pH 7.5 and 37°C


proof_lines: L-glutamine as ammonia source, pH 7.0, 25°C

proof_lines: ammonium sulfate as ammonia source, pH 7.0, 25°C

proof_lines: L-glutamine as ammonia source, in the presence of Mg2+, pH 7.0, 25°C


organism: Pseudomonas putida


enzyme_display_name: nucleoside phosphoacylhydrolase

substrate_name: guanosine 5'-p-nitrophenylphosphate

enzyme: nucleoside phosphoacylhydrolase

substrate: guanosine 5'-p-nitrophenylphosphate


enzyme_display_name: 6-methylsalicylate decarboxylase

organism: Penicillium griseofulvum

substrate_name: orsellinic acid

enzyme: 6-methylsalicylate decarboxylase

substrate: orsellinic acid


organism: Acinetobacter calcoaceticus

substrate_name: malonate

assay_conditions_summary: pH 6.8; 303.15 K; cofactor CoA

proof_lines: subunits beta/gamma in presence of acetyl-CoA

substrate: malonate


enzyme_display_name: 2-dehydro-3-deoxy-L-pentonate aldolase

organism: Pseudomonas sp.

enzyme: 2-dehydro-3-deoxy-L-pentonate aldolase


enzyme_display_name: prolycopene isomerase

organism: Solanum lycopersicum

substrate_name: Prolycopene

enzyme: prolycopene isomerase

substrate: Prolycopene


enzyme_display_name: lysine racemase

organism: Oenococcus oeni

substrate_name: D-Lysine

proof_lines: pH 8.0, 30°C, recombinant mutant T224I/W355Y

enzyme: lysine racemase

substrate: D-Lysine


enzyme_display_name: UniProt Q04HB7

substrate_name: L-lysine

enzyme: UniProt Q04HB7

substrate: L-lysine


organism: Proteus mirabilis

substrate_name: L-arginine

proof_lines: pH 9.0, 50°C, recombinant His-tagged mutant T391Y

substrate: L-arginine


proof_lines: pH 9.0, 50°C, recombinant His-tagged mutant T391Y/S394Y


proof_lines: pH 8.0, 50°C, recombinant wild-type enzyme

proof_lines: pH 9.0, 50°C, recombinant His-tagged wild-type enzyme


enzyme_display_name: L-Lysine = D-Lysine

organism: Thermotoga maritima

substrate_name: L-Lysine

enzyme: L-Lysine = D-Lysine

substrate: L-Lysine


organism: uncultured bacterium

proof_lines: in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C


organism: Lentinus sajor-caju

substrate_name: phosphate

proof_lines:
pH 7
pH 6.3

substrate: phosphate


organism: Pleurotus sajor-caju

substrate_name: Phosphate; alpha,alpha-Trehalose

substrate: Phosphate; alpha,alpha-Trehalose


organism: Schizophyllum commune

substrate_name: alpha-D-glucose 1-phosphate

proof_lines:
30°C, pH 6.6, recombinant enzyme
30°C, pH 6.6, natural enzyme

substrate: alpha-D-glucose 1-phosphate


enzyme_display_name: fucose-1-phosphate guanylyltransferase

organism: Sus scrofa

substrate_name: Diphosphate; GDP-L-fucose

enzyme: fucose-1-phosphate guanylyltransferase

substrate: Diphosphate; GDP-L-fucose


organism: Vitis vinifera

substrate_name: GTP

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: GTP


enzyme_display_name: bile-salt sulfotransferase

organism: Homo sapiens

enzyme: bile-salt sulfotransferase


enzyme_display_name: proline racemase

organism: Clostridioides difficile

proof_lines: substrate: D-proline, Vmax= 0.000017 M/sec

proof_lines: substrate: L-proline, Vmax = 0.000027 M/sec

enzyme: proline racemase


substrate_name: D-proline

substrate: D-proline


organism: Ferroplasma acidarmanus

substrate_name: L-proline

substrate: L-proline


organism: Thermococcus litoralis


organism: Trypanosoma cruzi

substrate_name: L-Proline

substrate: L-Proline


proof_lines:
pH 6.0, 37°C
pH 6.0, 37°C, TcPRACB
pH 6.0, 37°C, TcPRACA


organism: Trypanosoma vivax

proof_lines: 40 mM substrate in 0.2 M sodium acetate, for 30 min at 37°C

proof_lines: 0.005 mM pyrrole-2-carboxylic acid, serial concentrations of 20-160 mM L-proline


proof_lines: pH 8.0, 30°C, recombinant wild-type enzyme


organism: Unknown

substrate_name: GDP-L-fucose

substrate: GDP-L-fucose


substrate_name: trehalose (at pH 7.0)

substrate: trehalose (at pH 7.0)


organism: Arabidopsis thaliana

substrate_name: L-fucose for the L-fucokinase activity

substrate: L-fucose for the L-fucokinase activity


substrate_name: DHEA

substrate: DHEA


organism: Rattus norvegicus

substrate_name: 3'-phosphoadenylyl sulfate

substrate: 3'-phosphoadenylyl sulfate


substrate_name: alpha,alpha-trehalose

substrate: alpha,alpha-trehalose


proof_lines: wild-type, Kcat/Km (phosphate): 21000/Msec


proof_lines: mutant R507A, Kcat/Km (phosphate): 0.0004/Msec


substrate_name: Unknown

substrate: Unknown


organism: Agaricus bisporus

substrate_name: alpha-D-glucose

substrate: alpha-D-glucose


organism: Flammulina velutipes


organism: Lactobacillus acidophilus

proof_lines: loop 3 variant TP3, in 40 mM phosphate-citrate pH 6.2 at 45°C

proof_lines: loop 3 variant TP2, in 40 mM phosphate-citrate pH 6.2 at 45°C


organism: Pleurotus ostreatus


substrate_name: fucose 1-phosphate

substrate: fucose 1-phosphate


organism: Bacteroides fragilis

substrate_name: beta-L-fucose 1-phosphate

substrate: beta-L-fucose 1-phosphate


substrate_name: mannose 1-phosphate

substrate: mannose 1-phosphate


organism: Mortierella alpina


organism: Acetoanaerobium sticklandii

substrate_name: L-Pro

proof_lines: pH and temperature not specified in the publication

substrate: L-Pro


proof_lines: recombinant His-tagged enzyme, pH 6.0, 37°C


enzyme_display_name: phosphomethylpyrimidine synthase

organism: Salmonella enterica

proof_lines: pH 8.0, 37°C, recombinant His-tagged enzyme

enzyme: phosphomethylpyrimidine synthase


substrate_name: CoA

substrate: CoA


enzyme_display_name: 7,8-dihydroneopterin epimerase

organism: Escherichia coli

substrate_name: 7,8-dihydroneopterin triphosphate

enzyme: 7,8-dihydroneopterin epimerase

substrate: 7,8-dihydroneopterin triphosphate


organism: Haemophilus influenzae


enzyme_display_name: flavanone 3-dioxygenase

organism: Albuca bracteata

enzyme: flavanone 3-dioxygenase


organism: Oryza sativa

substrate_name: (S)-eriodictyol

substrate: (S)-eriodictyol


enzyme_display_name: guanidinopropionase

organism: Candida parapsilosis

proof_lines: 3-guanidinopropionase, pH and temperature not specified in the publication

enzyme: guanidinopropionase


organism: Pseudomonas aeruginosa

proof_lines: pH 7.9, 30°C, wild-type unmethylated GpuA


smiles: N=C(N)NCCCC(=O)[O-]


smiles: N=C(N)NCCC(=O)[O-]


proof_lines: pH 7.9, 30°C, mutant Y157M unmethylated GpuA


organism: Petunia x hybrida


organism: Petroselinum crispum


organism: Arabidopsis thaliana


organism: Artemisia annua

substrate_name: naringenin

proof_lines: pH 8.5, 35°C, recombinant His-tagged enzyme

substrate: naringenin


organism: Glycine max

substrate_name: eriodictyol

substrate: eriodictyol


substrate_name: dihydroquercetin

substrate: dihydroquercetin


organism: Escherichia coli (strain K12)

substrate_name: 7,8-dihydroneopterin in aldolase reaction


organism: Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

substrate: 7,8-dihydroneopterin in aldolase reaction


organism: Methanothermobacter thermautotrophicus

assay_conditions_summary: pH 8; 338.15 K; cofactor CoA

proof_lines: pH 8.0, 65°C, substrate phenylpyruvate, benzyl viologen as electron acceptor


organism: Pyrococcus furiosus

substrate_name: phenylpyruvate

assay_conditions_summary: pH 8.4; 353.15 K; cofactor CoA

proof_lines:
pH 8.4, 80°C
pH 8.4, 80°C, substrate phenylpyruvate
pH 8.4, 80°C, substrate indolepyruvate

substrate: phenylpyruvate


organism: Thermococcus profundus

substrate_name: oxidized ferredoxin

assay_conditions_summary: pH 9; 353.15 K; buffer 50 mM TAPS; cofactor CoA

proof_lines:
in 50 mM TAPS buffer (pH 9.0), at 80°C
pH 9.0, 80°C

substrate: oxidized ferredoxin


enzyme_display_name: stizolobate synthase

organism: Stizolobium hassjoo

assay_conditions_summary: cofactor NADP+

enzyme: stizolobate synthase


enzyme_display_name: scopoletin 8-hydroxylase

substrate_name: scopoletin

enzyme: scopoletin 8-hydroxylase

substrate: scopoletin


enzyme_display_name: [protein]-arginine 3-hydroxylase

organism: Homo sapiens

substrate_name: 2-Oxoglutarate; O2; RPS6-peptide

enzyme: [protein]-arginine 3-hydroxylase

substrate: 2-Oxoglutarate; O2; RPS6-peptide


organism: Streptomyces glaucescens

substrate_name: NADPH

assay_conditions_summary: pH 9; 298.15 K; cofactor NADPH

substrate: NADPH


organism: Sorghum bicolor

substrate_name: H+; NADPH; O2; 4-Hydroxyphenylacetaldoxime


enzyme_display_name: syn-pimaradiene 3-monooxygenase

organism: Oryza sativa

enzyme: syn-pimaradiene 3-monooxygenase


substrate_name: tetracenomycin A2

substrate: tetracenomycin A2


organism: Streptomyces globisporus

assay_conditions_summary: pH 6; 298.15 K; cofactor FAD


enzyme_display_name: vinorine hydroxylase

organism: Rauvolfia serpentina

substrate_name: vinorine

enzyme: vinorine hydroxylase

substrate: vinorine


enzyme_display_name: (+)-piperitol/(+)-sesamin synthase

organism: Sesamum indicum

substrate_name: (+)-pinoresinol

enzyme: (+)-piperitol/(+)-sesamin synthase

substrate: (+)-pinoresinol


enzyme_display_name: (+)-lariciresinol reductase

organism: Camellia sinensis var. sinensis

substrate_name: (+)-lariciresinol

enzyme: (+)-lariciresinol reductase

substrate: (+)-lariciresinol


enzyme_display_name: sulfide dehydrogenase

assay_conditions_summary: pH 8; 353.15 K; cofactor NADPH

enzyme: sulfide dehydrogenase


organism: Escherichia coli

substrate_name: cytosine967 in 16S rRNA

substrate: cytosine967 in 16S rRNA


enzyme_display_name: benzoylsuccinyl-CoA thiolase

organism: Geobacter metallireducens

substrate_name: succinyl-CoA

assay_conditions_summary: cofactor CoA

proof_lines: calculated, kinetic model: ternary complex mechanism, pH and temperature not specified in the publication

enzyme: benzoylsuccinyl-CoA thiolase

substrate: succinyl-CoA


enzyme_display_name: RING-type E3 ubiquitin transferase

proof_lines: 37°C, pH not specified in the publication

enzyme: RING-type E3 ubiquitin transferase


enzyme_display_name: sulfoacetaldehyde acetyltransferase

organism: Castellaniella defragrans

enzyme: sulfoacetaldehyde acetyltransferase


organism: Streptomyces niveus

substrate_name: demethyldecarbamoylnovobiocin

substrate: demethyldecarbamoylnovobiocin


enzyme_display_name: piperidine N-piperoyltransferase

organism: Piper nigrum

substrate_name: piperidine

enzyme: piperidine N-piperoyltransferase

substrate: piperidine


organism: uncultured bacterium


organism: Rattus norvegicus


substrate_name: sulfoacetaldehyde

substrate: sulfoacetaldehyde


organism: Allium cepa

substrate_name: 1-kestose (3a) (for 6G-FFT activity)

substrate: 1-kestose (3a) (for 6G-FFT activity)


organism: Saccharomyces cerevisiae


substrate_name: 1F(1-beta-D-fructofuranosyl)2 sucrose

substrate: 1F(1-beta-D-fructofuranosyl)2 sucrose


enzyme_display_name: ginsenoside 20-O-glucosyltransferase

organism: Panax ginseng

enzyme: ginsenoside 20-O-glucosyltransferase


enzyme_display_name: hydroxyjasmonate sulfotransferase

organism: Arabidopsis thaliana

enzyme: hydroxyjasmonate sulfotransferase


enzyme_display_name: caffeate CoA-transferase

organism: Acetobacterium woodii

assay_conditions_summary: pH 7.5; 313.15 K; cofactor CoA

enzyme: caffeate CoA-transferase


substrate_name: beta-D-glucopyranosyl abscisate

substrate: beta-D-glucopyranosyl abscisate


enzyme_display_name: pseudaminic acid synthase

organism: Campylobacter jejuni

enzyme: pseudaminic acid synthase


enzyme_display_name: tRNA nucleotidyltransferase

proof_lines: with tRNA-C-C-A-C2 or tRNA-C-C-A-C3, pH 8.0, 37°C

enzyme: tRNA nucleotidyltransferase


proof_lines: calculated as adenylyl groups removed from substrate


enzyme_display_name: all-trans-retinyl ester 13-cis isomerohydrolase

organism: Danio rerio

substrate_name: all-trans retinyl ester

substrate: all-trans retinyl ester


enzyme_display_name: hydroxymethylglutaryl-CoA hydrolase

proof_lines:
microsomal
soluble enzyme

enzyme: hydroxymethylglutaryl-CoA hydrolase


enzyme_display_name: fructose-2,6-bisphosphate 6-phosphatase

substrate_name: D-fructose 2,6-bisphosphate

assay_conditions_summary: pH 6

enzyme: fructose-2,6-bisphosphate 6-phosphatase

substrate: D-fructose 2,6-bisphosphate


organism: Hordeum vulgare

substrate_name: p-nitrophenyl-beta-D-glucopyranoside

substrate: p-nitrophenyl-beta-D-glucopyranoside


enzyme_display_name: 2,3-bisphosphoglycerate 3-phosphatase

organism: Homo sapiens

proof_lines: phosphatase activity of deglycosylated human HsMIPP1 protein

enzyme: 2,3-bisphosphoglycerate 3-phosphatase


organism: Bos taurus

substrate_name: UDP-D-xylose; Glc-beta-Ser(53)-EGF-like domain of bovine factor IX(45-87)


substrate_name: 11-cis retinol

enzyme: all-trans-retinyl ester 13-cis isomerohydrolase

substrate: 11-cis retinol


enzyme_display_name: galactan endo-1,6-beta-galactosidase

organism: Penicillium oxalicum

substrate_name: de-arabinosylated gum arabic

enzyme: galactan endo-1,6-beta-galactosidase

substrate: de-arabinosylated gum arabic


enzyme_display_name: ginsenoside 6-O-glucosyltransferase

enzyme: ginsenoside 6-O-glucosyltransferase


enzyme_display_name: lavandulyl diphosphate synthase

organism: Lavandula x intermedia

substrate_name: dimethylallyl diphosphate

enzyme: lavandulyl diphosphate synthase

substrate: dimethylallyl diphosphate


substrate_name: L-serine

substrate: L-serine


enzyme_display_name: funoran endo-alpha-hydrolase

organism: Aquimarina sp. AD1

substrate_name: funoran

enzyme: funoran endo-alpha-hydrolase

substrate: funoran


organism: Pseudomonas aeruginosa


organism: Artemisia spiciformis


organism: Escherichia coli (strain K12 / MC4100 / BW2952)

substrate_name: phosphate in the degradative reaction

substrate: phosphate in the degradative reaction


organism: Escherichia coli (strain K12)

substrate_name: adenylyl

assay_conditions_summary: cofactor ATP

substrate: adenylyl


substrate_name: gamma-glutamyl-gamma-aminobutyrate

substrate: gamma-glutamyl-gamma-aminobutyrate


substrate_name: Cys-Gly (at pH 6.9 and 25 degrees Celsius)

proof_lines: kcat is 3400 min(-1) for Cys-Gly hydrolysis activity (at pH 6.9 and 25 degrees Celsius). kcat is 11500 min(-1) for Leu-Gly hydrolysis activity (at pH 6.9 and 25 degrees Celsius). kcat is 8150 min(-1) for Met-Gly hydrolysis activity (at pH 6


enzyme_display_name: cyanophycinase

organism: Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

substrate_name: cyanophycin (at 30 degrees Celsius and pH 8.0)

enzyme: cyanophycinase


proof_lines: Values obtained using the partial C-terminal enzyme sequence of 105-377.


enzyme_display_name: guanidinopropionase

organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

proof_lines: kcat is 106 sec(-1) with 3-guanidinopropanoate as substrate. kcat is 57.6 sec(-1) for 4-guanidinobutanoate as substrate.

enzyme: guanidinopropionase


enzyme_display_name: aminodeoxychorismate lyase

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: aminodeoxychorismate

proof_lines: kcat is 1.9 sec(-1) with aminodeoxychorismate as substrate. kcat is 1.46 sec(-1) with D-alanine as substrate. kcat is 0.25 sec(-1) with D-glutamate as substrate.

enzyme: aminodeoxychorismate lyase

substrate: aminodeoxychorismate


enzyme_display_name: 3-hydroxybutyryl-CoA dehydratase

proof_lines: kcat is 110 sec(-1) with butenoyl-CoA as substrate.

enzyme: 3-hydroxybutyryl-CoA dehydratase


enzyme_display_name: 7,8-dihydroneopterin epimerase

substrate_name: 7,8-dihydroneopterin in the aldolase reaction

proof_lines: kcat is 0.0054 sec(-1) for the aldolase reaction with 7,8-dihydroneopterin as substrate. kcat is 0.006 sec(-1) for the aldolase reaction with 7,8-dihydromonapterin as substrate. kcat is 0.0015 sec(-1) for the epimerization of 7,8-dihydroneo

enzyme: 7,8-dihydroneopterin epimerase


enzyme_display_name: glucan 1,3-alpha-glucosidase

organism: Amorphotheca resinae

substrate_name: pseudonigeran

enzyme: glucan 1,3-alpha-glucosidase

substrate: pseudonigeran


organism: Trichoderma sp.


organism: Trichoderma asperellum

substrate_name: H2O; Mutan

substrate: H2O; Mutan


enzyme_display_name: chenodeoxycholoyltaurine hydrolase

organism: Bifidobacterium longum

substrate_name: glycocholate (at 37 degrees Celsius and pH 5.5)

proof_lines: kcat is 85 sec(-1) with glycocholate as substrate. kcat is 76 sec(-1) with taurocholate as substrate (at 40 degrees Celsius and pH 6.5).

enzyme: chenodeoxycholoyltaurine hydrolase


enzyme_display_name: 4-hydroxyphenylacetaldehyde synthase

organism: Rhodiola rosea

substrate_name: L-tyrosine

proof_lines: kcat is 4.91 sec(-1) with L-tyrosine as substrate.

enzyme: 4-hydroxyphenylacetaldehyde synthase

substrate: L-tyrosine


substrate_name: CAPA

proof_lines: kcat is 2.36 sec(-1) with CAPA as substrate.

substrate: CAPA


organism: Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

proof_lines: kcat is 0.6 sec(-1) for 3-hydroxy-2-methylpyridine-4,5-dicarboxylate as substrate.


organism: Homo sapiens

substrate_name: Gppp-BODIPY

substrate: Gppp-BODIPY


organism: Petroselinum crispum


enzyme_display_name: siroheme decarboxylase

organism: Desulfovibrio desulfuricans (strain ATCC 27774 / DSM 6949 / MB)

substrate_name: siroheme

enzyme: siroheme decarboxylase

substrate: siroheme


organism: Synechocystis sp. PCC 6803

proof_lines: at pH 7.4, temperature not specified in the publication


organism: Desulfovibrio desulfuricans

proof_lines: pH and temperature not specified in the publication


organism: Mesorhizobium loti

proof_lines: in 100 mM Tris-HCl at pH 8.0 containing 100 mM NaCl and 2 mM dithiothreitol


organism: Pseudomonas sp.


organism: Nocardioides sp.


enzyme_display_name: sulfofructosephosphate aldolase

organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: 6-deoxy-6-sulfo-D-fructose 1-phosphate

enzyme: sulfofructosephosphate aldolase

substrate: 6-deoxy-6-sulfo-D-fructose 1-phosphate


organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

proof_lines: kcat is 47.7 sec(-1) with 6-deoxy-6-sulfo-D-fructose 1-phosphate as substrate.


enzyme_display_name: 2-halobenzoate 1,2-dioxygenase

organism: Burkholderia cepacia

assay_conditions_summary: cofactor NADH

proof_lines:
component A
component B

enzyme: 2-halobenzoate 1,2-dioxygenase


proof_lines: cells grown on 2-chlorobenzoate or 2,5-dichlorobenzoate


enzyme_display_name: 1-hydroxy-2-naphthoate hydroxylase

organism: Alcaligenes sp.

substrate_name: NADH

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADPH

proof_lines:
pH 7.5, 30°C
pH 7.5, 30°C, with NADPH
pH 7.5, 30°C, with NADH

enzyme: 1-hydroxy-2-naphthoate hydroxylase

substrate: NADH


enzyme_display_name: 4-hydroxyphenylacetate 1-monooxygenase

organism: Delftia acidovorans

enzyme: 4-hydroxyphenylacetate 1-monooxygenase


enzyme_display_name: propanediol-phosphate dehydrogenase

organism: Oryctolagus cuniculus

enzyme: propanediol-phosphate dehydrogenase


organism: uncultured bacterium


enzyme_display_name: peptide-tryptophan 2,3-dioxygenase

organism: Triticum aestivum

substrate_name: N-acetyltryptophan

enzyme: peptide-tryptophan 2,3-dioxygenase

substrate: N-acetyltryptophan


organism: Zea mays

assay_conditions_summary: pH 8.5; 308.15 K; cofactor NADPH

proof_lines: recombinant enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C

proof_lines: native enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C


enzyme_display_name: 2-nitrophenol 2-monooxygenase

organism: Pseudomonas putida

assay_conditions_summary: cofactor NADPH

enzyme: 2-nitrophenol 2-monooxygenase


enzyme_display_name: marmesin synthase

organism: Ficus carica

substrate_name: demethylsuberosin

enzyme: marmesin synthase

substrate: demethylsuberosin


enzyme_display_name: indolin-2-one monooxygenase

enzyme: indolin-2-one monooxygenase


organism: Ammi majus


enzyme_display_name: adenosylcobinamide kinase

assay_conditions_summary: pH 8.8; 310.15 K; cofactor ATP

enzyme: adenosylcobinamide kinase


enzyme_display_name: ginsenoside 20-O-glucosyltransferase

organism: Panax ginseng

substrate_name: protopanaxadiol

proof_lines: kcat is 9.4 sec(-1) with protopanaxadiol as substrate (PubMed:24603359). kcat is 12.7 sec(-1) with dammarenediol II as substrate (PubMed:24603359).

enzyme: ginsenoside 20-O-glucosyltransferase

substrate: protopanaxadiol


enzyme_display_name: pseudaminic acid synthase

organism: Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)

substrate_name: phosphoenolpyruvate

verification_status: unverified

enzyme: pseudaminic acid synthase

substrate: phosphoenolpyruvate

status: unverified


enzyme_display_name: fucose-1-phosphate guanylyltransferase

organism: Bacteroides fragilis

substrate_name: L-fucose

proof_lines: kcat is 0.39 sec(-1) for the fucokinase activity. kcat is 10.07 sec(-1) for the fucose-1-phosphate guanylyltransferase activity.

enzyme: fucose-1-phosphate guanylyltransferase

substrate: L-fucose


organism: Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)

substrate_name: DGGGP

substrate: DGGGP


organism: Escherichia coli (strain K12)

substrate_name: CDP-1,2-diacyl-sn-glycerol (at pH 7.5 and 25 degrees Celsius)

proof_lines: kcat is 69.7 sec(-1) for the CDP-1,2-diacyl-sn-glycerol and 59.8 sec(-1) for L-serine (at pH 7.5 and 25 degrees Celsius).


enzyme_display_name: caffeate CoA-transferase

organism: Acetobacterium woodii

substrate_name: hydrocaffeyl-CoA

proof_lines: kcat is 120 sec(-1) for transferase activity with caffeate as substrate.

enzyme: caffeate CoA-transferase

substrate: hydrocaffeyl-CoA


enzyme_display_name: all-trans-retinyl ester 13-cis isomerohydrolase

organism: Danio rerio

substrate_name: all-trans-retinyl ester

proof_lines: kcat is 0.00044 sec(-1) with all-trans-retinyl ester as substrate.

substrate: all-trans-retinyl ester


enzyme_display_name: acetoacetyl-CoA hydrolase

organism: Mus musculus

substrate_name: acetyl-CoA

proof_lines: In summary, KM for medium- to long-chain acyl CoAs is in the order 1.4-6.7 uM, and with short-chain acyl CoAs range from 8 to 30 uM. KM for bile acid-CoA esters is in the range 6-15 uM.

enzyme: acetoacetyl-CoA hydrolase

substrate: acetyl-CoA


enzyme_display_name: hydroxymethylglutaryl-CoA hydrolase

enzyme: hydroxymethylglutaryl-CoA hydrolase


enzyme_display_name: kanosamine-6-phosphate phosphatase

organism: Bacillus subtilis (strain 168)

proof_lines: kcat is 32 sec(-1) for dephosphorylation with kanosamine 6-phosphate (at pH 7.5 and 25 degrees Celsius).

enzyme: kanosamine-6-phosphate phosphatase


enzyme_display_name: funoran endo-alpha-hydrolase

organism: Aquimarina sp. (strain AD1)

substrate_name: funoran

proof_lines: kcat is 43.40 sec(-1) with funoran as substrate. kcat is 101.39 sec(-1) with agarose as substrate.

enzyme: funoran endo-alpha-hydrolase

substrate: funoran


organism: Bacillus subtilis

substrate_name: ginsenoside Rc

substrate: ginsenoside Rc


substrate_name: 4-nitrophenyl alpha-L-arabinofuranoside

substrate: 4-nitrophenyl alpha-L-arabinofuranoside


organism: Bifidobacterium longum


assay_conditions_summary: pH 5.5; 310.15 K; buffer 50 mM sodium acetate

proof_lines: in 50 mM sodium acetate buffer (pH 5.5), at 37°C


organism: Bifidobacterium longum subsp. Longum

substrate_name: larch type II arabinogalactan

substrate: larch type II arabinogalactan


organism: Chaetomium sp.


organism: Corynebacterium alkanolyticum


organism: Evansstolkia leycettana


organism: Geobacillus stearothermophilus


organism: Mycosarcoma maydis


organism: Acetivibrio thermocellus

proof_lines: pH 5.7, 50°C, recombinant full-length enzyme

proof_lines: pH 5.7, 50°C, recombinant catalytic module CtGH43


organism: Alicyclobacillus sp.


organism: Anoxybacillus kestanbolensis

substrate_name: p-nitrophenyl-alpha-L-arabinofuranoside

substrate: p-nitrophenyl-alpha-L-arabinofuranoside


organism: Aspergillus nidulans

substrate_name: rye arabinoxylan

substrate: rye arabinoxylan


organism: Aspergillus niger

assay_conditions_summary: pH 5; 323.15 K; buffer 50 mM acetate

proof_lines: in 50 mM acetate buffer, pH 5.0, at 50°C


organism: Aureobasidium pullulans


organism: Bacillus pumilus

substrate_name: p-nitrophenyl alpha-L-arabinofuranoside

substrate: p-nitrophenyl alpha-L-arabinofuranoside


substrate_name: linear alpha-1,5-L-arabinan

substrate: linear alpha-1,5-L-arabinan


enzyme_display_name: catalase peroxidase

organism: Mycobacterium tuberculosis

substrate_name: o-Dianisidine

proof_lines:
wild type enzyme, at pH 4.5 and 30°C
wild type enzyme, at pH 7.0 and 30°C

enzyme: catalase peroxidase

substrate: o-Dianisidine


organism: Neurospora crassa

proof_lines: pH 5.0, 25°C, recombinant intein-tagged enzyme

proof_lines: pH 5.5, 25°C, recombinant intein-tagged enzyme

proof_lines: pH 5.0, 25°C, with o-dianisidine, recombinant intein-tagged enzyme


organism: Pyricularia grisea


proof_lines: mutant C26A/C74A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant C55A/C74A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant C55A, pH not specified in the publication, temperature not specified in the publication


proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant C74A, pH not specified in the publication, temperature not specified in the publication


organism: Synechococcus elongatus

substrate_name: isoniazid

substrate: isoniazid


organism: Synechocystis sp.

assay_conditions_summary: pH 7; 298.15 K; buffer 25 mM HEPES-NaOH

proof_lines: wild type enzyme, in 25 mM HEPES-NaOH buffer (pH 7.0) at 25°C


organism: Talaromyces funiculosus

substrate_name: wheat arabinoxylan

proof_lines:
pH 4.0, 40°C, isozyme PfABF62a
pH 4.0, 40°C, isozyme PfABF62b
pH 4.0, 40°C, isozyme PfABF62c

substrate: wheat arabinoxylan


proof_lines: pH 4.0, 40°C, isozyme mutant pfabf62cDELTACBM


organism: Aspergillus terreus

proof_lines:
isoenzyme ABF1
isoenzyme ABF2
isoenzyme ABF3


organism: Fusarium oxysporum

proof_lines:
pH 6.0, 45°C, isozyme ABF2
pH 6.0, 45°C, isozyme ABF1


organism: Streptomyces diastaticus

substrate_name: phenyl-alpha-L-arabinofuranoside

proof_lines:
isoenzyme C2
isoenzyme C1

substrate: phenyl-alpha-L-arabinofuranoside


organism: Trichoderma reesei

proof_lines:
isoenzyme AF53
isoenzyme AF35


organism: Unknown

substrate_name: arabinobiose

substrate: arabinobiose


organism: Paenibacillus sp.

proof_lines: recombinant His6-tagged enzyme, pH 6.0, 60°C


organism: Penicillium parvum


organism: Pleurotus ostreatus


organism: Podospora anserina


organism: Pseudothermotoga thermarum


organism: Streptomyces coelicolor

substrate_name: wheat L-arabinoxylan

proof_lines: ph 5.5, 37°C, recombinant wild-type enzyme

proof_lines: pH 5.5, 37°C, recombinant wild-type enzyme

substrate: wheat L-arabinoxylan


substrate_name: L-arabinan

substrate: L-arabinan


organism: Talaromyces purpureogenus


assay_conditions_summary: pH 4; 303.15 K; buffer 50 mM sodium citrate

proof_lines: in 50 mM sodium citrate buffer (pH 4.0), at 30°C


organism: Thermobacillus xylanilyticus


proof_lines:
wild-type, pH 7.0, 45°C
pH 5.8, 60°C, wild-type enzyme