organism: Panicum miliaceum

proof_lines: isozyme cAspAT, alpha-form, pH 8.0, 25°C

proof_lines: isozyme cAspAT, gamma-form, pH 8.0, 25°C

proof_lines: isozyme pAsp-AT, beta-form, pH 8.0, 25°C


enzyme_display_name: aspartate transaminase

organism: Rattus norvegicus

substrate_name: L-aspartate

source_db: brenda

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

source_license: CC BY 4.0

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: enzyme from nonsynaptic mitochondrion, pH 7.8, 30°C

enzyme: aspartate transaminase

substrate: L-aspartate

source: brenda


organism: Sus scrofa

substrate_name: Unknown

km_display: Not reported

ki_unit: mM

substrate: Unknown


enzyme_display_name: branched-chain-amino-acid transaminase

organism: Canis lupus familiaris

proof_lines: pH 8.0, 37°C, isoenzyme III, leucine as amino group donor

proof_lines: pH 8.0, 37°C, isoenzyme I, isoleucine as amino group donor

proof_lines: pH 8.0, 37°C, isoenzyme III, 2-oxoglutarate as amino group acceptor

enzyme: branched-chain-amino-acid transaminase


organism: Homo sapiens

substrate_name: L-Glutamate; 4-Methyl-2-oxopentanoate

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

proof_lines: TAT-peptide fusion protein, pH 7.4, 25°C

proof_lines: pH 8.4, 25°C, substrate L-alloisoleucine

proof_lines: pH 8.0, 37°C, isoenzyme III, valine as amino group donor

substrate: L-Glutamate; 4-Methyl-2-oxopentanoate


organism: Saccharomyces cerevisiae

substrate_name: beta-D-fructose 6-phosphate

assay_conditions_summary: pH 7.1; 303.15 K; cofactor ATP

proof_lines: pH 7.1, 30°C, kinetic data of various enzyme forms

substrate: beta-D-fructose 6-phosphate


organism: Unknown

assay_conditions_summary: cofactor ATP


enzyme_display_name: (S)-hydroxynitrile lyase

organism: Hevea brasiliensis

substrate_name: acetone cyanohydrin

protein_accession_database: UniProt

source_license: CC BY 4.0; CC BY-NC-ND 4.0

proof_lines:
pH 5.4
isoform HNL, at pH 7.2 and 25°C
Raw SKiD Env: Temp=-----, pH=-----

source_databases_merged: brenda skid

enzyme: (S)-hydroxynitrile lyase

substrate: acetone cyanohydrin


organism: Manihot esculenta

substrate_name: Acetone cyanohydrin

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

substrate: Acetone cyanohydrin


substrate_name: Mandelonitrile

substrate: Mandelonitrile


substrate_name: (S)-mandelonitrile

assay_conditions_summary: pH 5; 296.15 K; buffer pH settings and a separate pH

proof_lines: wild type enzyme, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication

proof_lines: wild type enzyme, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication

substrate: (S)-mandelonitrile


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution

assay_conditions_summary: pH 5; buffer pH above 6

proof_lines: mutant enzyme H103L, in 100 mM citrate buffer pH above 6.0, temperature not specified in the publication

proof_lines: mutant enzyme H103L, in 100 mM citrate buffer pH 5.0, temperature not specified in the publication


substrate_name: 2-thiophene carboxaldehyde

source_db: oed

substrate: 2-thiophene carboxaldehyde

source: oed


substrate_name: 4-biphenyl carboxaldehyde

substrate: 4-biphenyl carboxaldehyde


substrate_name: 1-naphthalene carboxaldehyde

substrate: 1-naphthalene carboxaldehyde


enzyme_display_name: dihydrolipoyl dehydrogenase

organism: Plasmodium falciparum

substrate_name: NAD+

assay_conditions_summary: pH 8; 298.15 K; cofactor NAD+

proof_lines: pH 8.0, 25°C, recombinant His-tagged enzyme

proof_lines: pH 7.0, 25°C, recombinant His-tagged enzyme

proof_lines: pH 8.0, 25°C, mitochondrial isozyme, forward reaction, varying conditions

enzyme: dihydrolipoyl dehydrogenase

substrate: NAD+


enzyme_display_name: sulfite oxidase

substrate_name: sulfite

enzyme: sulfite oxidase

substrate: sulfite


substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


organism: Zea mays

substrate_name: shikimate 3-phosphate

proof_lines:
isoenzyme II
isoenzyme I
isoenzyme II, vs. shikimate 3-phosphate

substrate: shikimate 3-phosphate


organism: Arabidopsis thaliana

proof_lines:
isozyme AAT2, pH 8.0, 25°C
isozyme AAT5, pH 8.0, 25°C
isozyme AAT1, pH 8.0, 25°C


organism: Atriplex spongiosa

assay_conditions_summary: pH 8

proof_lines:
isozyme Asp-DEAE-1, pH 8.0
isozyme Asp-DEAE-2, pH 8.0


proof_lines:
cytosolic isozyme
mitochondrial isozyme


organism: Eleusine coracana

proof_lines:
isozyme AspAT-3, 25°C, pH 8.0
isozyme AspAT-1, 25°C, pH 8.0


organism: Gallus gallus

proof_lines: recombinant premature isozyme pmAspAT, pH 7.5, 25°C

proof_lines: recombinant isozyme mAspAT, pH 7.5, 25°C


organism: Hyalomma dromedarii


organism: Lupinus albus

substrate_name: L-Aspartate; 2-Oxoglutarate

proof_lines: isozyme AAT2, competitive versus 2-oxoglutarate

substrate: L-Aspartate; 2-Oxoglutarate


organism: Baliospermum montanum


substrate_name: (R)-mandelonitrile

substrate: (R)-mandelonitrile


substrate_name: (S)-Mandelonitrile

substrate: (S)-Mandelonitrile


substrate_name: Cyanide

smiles: C#N

substrate: Cyanide


smiles: CC(C)(O)C#N


enzyme_display_name: subtilisin

substrate_name: Glp-Ala-Ala-Leu-p-Nitroanilide; H2O

enzyme: subtilisin

substrate: Glp-Ala-Ala-Leu-p-Nitroanilide; H2O


enzyme_display_name: arginase

substrate_name: L-arginine

enzyme: arginase

substrate: L-arginine


substrate_name: fructose 6-phosphate

source_db: uniprot

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

substrate: fructose 6-phosphate

source: uniprot


organism: Rattus norvegicus

substrate_name: beta-D-fructose 6-phosphate

substrate: beta-D-fructose 6-phosphate


enzyme_display_name: acetate kinase

organism: Methanosarcina thermophila

substrate_name: ATP

enzyme: acetate kinase

substrate: ATP


organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)


enzyme_display_name: adenylate kinase

organism: Bordetella pertussis (strain ATCC 9797 / DSM 5571 / CCUG 30873 / LMG 14455 / NCTC 10739 / 18323)

enzyme: adenylate kinase


organism: Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM)

substrate_name: AMP (at pH 7.5)

assay_conditions_summary: pH 7.5; cofactor ATP

substrate: AMP (at pH 7.5)


organism: Escherichia coli (strain K12)

substrate_name: ATP (at pH 7.4 and 30 degrees Celsius)

assay_conditions_summary: pH 7.4; cofactor ATP

substrate: ATP (at pH 7.4 and 30 degrees Celsius)


organism: Homo sapiens

substrate_name: AMP (with ATP as phosphate donor)

substrate: AMP (with ATP as phosphate donor)


substrate_name: AMP

substrate: AMP


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)


organism: Spirochaeta sp.

substrate_name: acetate

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

proof_lines:
pH 7.5, 30°C, isoenzyme II
pH 7.5, 30°C, isoenzyme I

substrate: acetate


organism: Unknown


organism: Mus musculus

proof_lines: isoenzyme AK1b, membrane protein fraction

proof_lines: isoenzyme AK1, membrane protein fraction

proof_lines: isoenzyme AK1, cytosolic protein fraction


enzyme_display_name: alkaline phosphatase

organism: Aspergillus oryzae

substrate_name: p-nitrophenyl phosphate

proof_lines:
isoenzyme III
isoenzyme I
isoenzyme II

enzyme: alkaline phosphatase

substrate: p-nitrophenyl phosphate


substrate_name: H2O; 4-Nitrophenyl phosphate

proof_lines: allozyme D, pH 7.5, 37°C, in presence of Mg2+ and Zn2+

proof_lines: allozyme D, pH 9.8, in presence of Mg2+ and Zn2+

substrate: H2O; 4-Nitrophenyl phosphate


organism: Bos taurus

substrate_name: H2O; AMP

proof_lines:
isoenzyme 5-NT-3
isoenzyme 5-NT-1
isoenzyme 5-NT-2 and 5-NT-4

substrate: H2O; AMP


enzyme_display_name: alpha-amylase

organism: Sus scrofa

proof_lines:
25°C, pH 7.2
isozyme PPA-II, at pH 6.9 and 30°C
isozyme PPA-I, at pH 6.9 and 30°C

enzyme: alpha-amylase


enzyme_display_name: alpha-galactosidase

organism: Aspergillus niger

substrate_name: H2O; Raffinose

proof_lines:
pH 4.5, 50°C, isoenzyme alpha-Gal I
pH 4.5, 50°C, isoenzyme alpha-Gal II
pH 4.5, 50°C, isoenzyme alpha-Gal III

enzyme: alpha-galactosidase

substrate: H2O; Raffinose


organism: Phlebia radiata

substrate_name: p-nitrophenyl alpha-D-galactopyranoside

proof_lines:
isoenzyme AgaS-m2
isoenzyme AgaS-b2
isoenzyme AgaS-b3

substrate: p-nitrophenyl alpha-D-galactopyranoside


substrate_name: raffinose

substrate: raffinose


organism: Melanocarpus albomyces

substrate_name: birchwood xylan

proof_lines:
isozyme IIa, pH 6.0, 70°C
isozyme Ia, pH 6.0, 70°C
isozyme IId, pH 6.0, 70°C

substrate: birchwood xylan


organism: Trichoderma sp.

proof_lines: pH 5.6, 50°C, isozyme EX1, oat spelt xylan

proof_lines: pH 5.6, 50°C, isozyme EX1, beechwood xylan

proof_lines: pH 5.6, 50°C, isozyme EX2, birchwood xylan


enzyme_display_name: cysteine synthase

organism: Arabidopsis thaliana

substrate_name: O-acetylserine (at pH 7.0 and 25 degrees Celsius) for the cysteine synthase activity

enzyme: cysteine synthase


substrate_name: L-cysteine for the cystathionine gamma-lyase activity


organism: Caenorhabditis elegans

substrate_name: O-acetylserine

substrate: O-acetylserine


substrate_name: O-acetylserine (at 25 degrees Celsius and pH 7.5)


substrate: O-acetylserine (at 25 degrees Celsius and pH 7.5)


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: O-acetyl-L-homoserine (OAH) (at 30 degrees Celsius in potassium phosphate buffer)

assay_conditions_summary: buffer potassium phosphate


substrate_name: O-acetyl-L-homoserine

substrate: O-acetyl-L-homoserine


organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

substrate_name: O-acetyl-L-serine as isolated subunit and in complex with CysK


organism: Solanum tuberosum

substrate_name: L-cysteine for the L-3-cyanoalanine synthase activity (in the presence of 5 mM KCN)


substrate_name: O(3)-acetyl-L-serine for the cysteine synthase activity (in the presence of 2.5 mM Na(2)S)


organism: Spinacia oleracea

substrate_name: O(3)-acetyl-L-serine for the cysteine synthase activity with the recombinant enzyme


organism: Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534)

substrate_name: lipoate

assay_conditions_summary: cofactor NADH

substrate: lipoate


organism: Hymenolepis diminuta

substrate_name: NADH for the NADH -> NAD reaction

substrate: NADH for the NADH -> NAD reaction


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: NAD(+)

substrate: NAD(+)


organism: Thermus scotoductus (strain ATCC 700910 / SA-01)

substrate_name: Cr(VI)

substrate: Cr(VI)


enzyme_display_name: thymidylate synthase

substrate_name: dUMP

enzyme: thymidylate synthase

substrate: dUMP


enzyme_display_name: glycine hydroxymethyltransferase

substrate_name: L-serine (at pH 6.5 and 30 degrees Celsius)

enzyme: glycine hydroxymethyltransferase


substrate: L-serine (at pH 6.5 and 30 degrees Celsius)


organism: Homo sapiens

substrate_name: L-serine (with HEPES)

substrate: L-serine (with HEPES)


substrate_name: L-serine

substrate: L-serine


organism: Oryctolagus cuniculus

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


organism: Ovis aries


enzyme_display_name: glycogen phosphorylase

organism: Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C)

substrate_name: maltoheptaose (at 60 degrees Celsius)

enzyme: glycogen phosphorylase

substrate: maltoheptaose (at 60 degrees Celsius)


organism: Rhizobium meliloti

substrate_name: aspartate

substrate: aspartate


substrate_name: L-aspartate

substrate: L-aspartate


substrate_name: 4-methyl-2-oxopentanoate (4MOP)

substrate: 4-methyl-2-oxopentanoate (4MOP)


substrate_name: L-leucine

km_unit: mM

data_origin: experimental

substrate: L-leucine


organism: Bacillus subtilis (strain 168)

substrate_name: isoleucine (with 10 mM ketomethiobutyrate)


substrate: isoleucine (with 10 mM ketomethiobutyrate)


organism: Humulus lupulus

substrate_name: glutamate

assay_conditions_summary: cofactor CoA

substrate: glutamate


organism: Rattus norvegicus


organism: Arabidopsis thaliana

substrate_name: fructose-6-phosphate for the 6-phosphofructo-2-kinase activity (at pH 6.0 and 25 degrees Celsius)


substrate_name: beta-D-fructose 6-phosphate

substrate: beta-D-fructose 6-phosphate


organism: Elizabethkingia meningoseptica

substrate_name: 4-nitrophenyl phosphate

substrate: 4-nitrophenyl phosphate


organism: Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805)


substrate_name: myo-inositol phosphate

substrate: myo-inositol phosphate


organism: Bos taurus

substrate_name: IMP (in absence of allosteric activator)


organism: Escherichia coli (strain K12)


substrate_name: UMP (at pH 7 and at 25 degrees Celsius)

assay_conditions_summary: pH 7

substrate: UMP (at pH 7 and at 25 degrees Celsius)


substrate_name: IMP (with manganese ions as cofactor and at pH 9)

assay_conditions_summary: pH 9


organism: Gallus gallus

substrate_name: IMP

substrate: IMP


substrate_name: AMP

substrate: AMP


substrate_name: IMP (in presence of the allosteric activator ATP)


substrate_name: m(7)GMP (at 37 degrees Celsius)

substrate: m(7)GMP (at 37 degrees Celsius)


organism: Sus scrofa

substrate_name: ThDP

assay_conditions_summary: cofactor ADP

substrate: ThDP


substrate_name: NMN

substrate: NMN


organism: Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)


substrate_name: beta-nicotinamide D-ribonucleotide

assay_conditions_summary: cofactor NAD+

substrate: beta-nicotinamide D-ribonucleotide


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: ATP

substrate: ATP


substrate_name: nicotinamide mononucleotide

substrate: nicotinamide mononucleotide


organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

substrate_name: ribosylnicotinamide

substrate: ribosylnicotinamide


enzyme_display_name: acetylcholinesterase

organism: Bungarus fasciatus

substrate_name: acetylcholine (at pH 7.5)

enzyme: acetylcholinesterase

substrate: acetylcholine (at pH 7.5)


organism: Caenorhabditis elegans

substrate_name: acetylthiocholine

substrate: acetylthiocholine


organism: Danio rerio

substrate_name: acetylcholine

substrate: acetylcholine


organism: Spinacia oleracea

substrate_name: O-acetylserine

substrate: O-acetylserine


organism: Streptomyces lavendulae

substrate_name: OAS

substrate: OAS


enzyme_display_name: protein farnesyltransferase

substrate_name: CVIM substrate and for farnesyl transferase activity

enzyme: protein farnesyltransferase


enzyme_display_name: aspartate transaminase

substrate_name: L-aspartate

enzyme: aspartate transaminase

substrate: L-aspartate


substrate_name: aspartate for the asparte aminotransferase activity


organism: Comamonas testosteroni


organism: Klebsiella pneumoniae

substrate_name: tryptophan (at pH 7.4 and 10 mM KMTB)

substrate: tryptophan (at pH 7.4 and 10 mM KMTB)


organism: Mus musculus


organism: Petunia hybrida

substrate_name: prephenate (with 20 mM aspartate as cosubstrate)


organism: Pinus pinaster

substrate_name: aspartate

substrate: aspartate


organism: Pseudomonas sp


organism: Rattus norvegicus

substrate_name: L-cysteine

substrate: L-cysteine


enzyme_display_name: orotidine-5'-phosphate decarboxylase

organism: Saccharomyces cerevisiae

substrate_name: orotidine 5'-phosphate

enzyme: orotidine-5'-phosphate decarboxylase

substrate: orotidine 5'-phosphate


proof_lines:
pH 7.1, 25°C, mutant Y217F
mutant Y217F, pH 7.1, 25°C


substrate_name: 5-Fluoroorotidine 5'-phosphate

substrate: 5-Fluoroorotidine 5'-phosphate


enzyme_display_name: hypoxanthine phosphoribosyltransferase

organism: Homo sapiens

substrate_name: IMP

enzyme: hypoxanthine phosphoribosyltransferase

substrate: IMP


substrate_name: hypoxanthine

substrate: hypoxanthine


organism: Agrobacterium sp. (strain CP4)

substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


organism: Escherichia coli (strain K12)

substrate_name: S3P (at pH 7 and 25 degrees Celsius)

substrate: S3P (at pH 7 and 25 degrees Celsius)


organism: Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)

substrate_name: PEP (with 100 mM ammonium at pH 7 and 25 degrees Celsius)

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


substrate_name: FPP (at pH 7.5 and at 25 degrees Celsius)


organism: Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)

substrate_name: FPP

substrate: FPP


organism: Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)

substrate_name: O(3)-acetyl-L-serine (in the presence of 1 mM sodium sulfide at pH 6.7 and 60 degrees Celsius)


organism: Arabidopsis thaliana

substrate_name: O(3)-acetyl-L-serine for the cysteine synthase activity


substrate: O(3)-acetyl-L-serine for the cysteine synthase activity


organism: Methanothermobacter thermautotrophicus


substrate_name: Orotidine 5'-phosphate

substrate: Orotidine 5'-phosphate


enzyme_display_name: (S)-hydroxynitrile lyase

organism: Baliospermum montanum

enzyme: (S)-hydroxynitrile lyase


substrate_name: Undecanal

smiles: CCCCCCCCCCC=O

substrate: Undecanal


substrate_name: piperonal

substrate: piperonal


substrate_name: Benzaldehyde; Cyanide

substrate: Benzaldehyde; Cyanide


substrate_name: benzaldehyde

substrate: benzaldehyde


substrate_name: Isophthaldehyde

substrate: Isophthaldehyde


smiles: CC(=O)CCC(C)C


substrate_name: Heptanal

smiles: CCCCCCC=O

substrate: Heptanal


substrate_name: Terephthalaldehyde

substrate: Terephthalaldehyde


substrate_name: Mandelonitrile

substrate: Mandelonitrile


smiles: CCC/C=C/C=O


substrate_name: 1-naphthalene carboxaldehyde

substrate: 1-naphthalene carboxaldehyde


substrate_name: 2-thiophene carboxaldehyde

substrate: 2-thiophene carboxaldehyde


proof_lines:
pH 4.2, 22°C
pH 6.0, 25°C
Raw SKiD Env: Temp=22°C, pH=4.2


substrate_name: (R)-mandelonitrile

substrate: (R)-mandelonitrile


substrate_name: 5-Fluoroorotidine 5'-phosphate

substrate: 5-Fluoroorotidine 5'-phosphate


source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

proof_lines:
pH 7.4, 25°C
Raw SKiD Env: Temp=25°C, pH=7.2
Raw SKiD Env: Temp=25°C, pH=7.1

source_databases_merged: sabio_rk brenda skid


enzyme_display_name: phosphoenolpyruvate carboxykinase (GTP)

organism: Ascaris suum

substrate_name: Phosphoenolpyruvate; IDP; CO2

enzyme: phosphoenolpyruvate carboxykinase (GTP)

substrate: Phosphoenolpyruvate; IDP; CO2


organism: Gallus gallus

substrate_name: Phosphoenolpyruvate; CO2; IDP

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

substrate: Phosphoenolpyruvate; CO2; IDP


substrate_name: CO2; Phosphoenolpyruvate; GDP

proof_lines:
CO2 in form of HCO3-
Raw SKiD Env: Temp=-----, pH=-----

substrate: CO2; Phosphoenolpyruvate; GDP


organism: Mus musculus

substrate_name: ITP; Oxaloacetate

substrate: ITP; Oxaloacetate


organism: Mycobacterium smegmatis


substrate_name: GTP; Oxaloacetate

substrate: GTP; Oxaloacetate


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)


substrate_name: IMP

substrate: IMP


substrate_name: p-nitrophenyl phosphate (at pH 5.4 and 37 degrees Celsius)


organism: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

substrate_name: pNPP (at 76 degrees Celsius)

substrate: pNPP (at 76 degrees Celsius)


organism: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

substrate_name: maltose (at pH 6.8 and 37 degrees Celsius)


enzyme_display_name: alpha-glucosidase

organism: Erwinia rhapontici

substrate_name: maltose

enzyme: alpha-glucosidase

substrate: maltose


organism: Homo sapiens

substrate_name: maltoheptaose (with ctMGAM)

substrate: maltoheptaose (with ctMGAM)


substrate_name: p-nitrophenyl-alpha-D-glucopyranoside

substrate: p-nitrophenyl-alpha-D-glucopyranoside


organism: Bacillus subtilis (strain 168)

substrate_name: melibiose

substrate: melibiose


organism: Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019 / LMG 10263 / NCTC 9343 / Onslow / VPI 2553 / EN-2)

substrate_name: Galalpha-pNP

substrate: Galalpha-pNP


organism: Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)


organism: Aspergillus oryzae (strain ATCC 42149 / RIB 40)

substrate_name: birch wood xylan

substrate: birch wood xylan


assay_conditions_summary: pH 4.6; 310.15 K; buffer 0.1 M sodium citrate

proof_lines: mutant enzyme A20P, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


proof_lines: mutant enzyme E66Q, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


proof_lines: mutant enzyme N215S, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


proof_lines: mutant enzyme A97V, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


enzyme_display_name: versatile peroxidase

organism: Amycolatopsis sp.

substrate_name: H2O2; Reactive black 5

enzyme: versatile peroxidase

substrate: H2O2; Reactive black 5


organism: Phanerochaete chrysosporium

substrate_name: H2O2; 2,6-Dimethoxyphenol

substrate: H2O2; 2,6-Dimethoxyphenol


organism: Pleurotus eryngii


substrate_name: Reactive black 5

substrate: Reactive black 5


proof_lines:
pH 3.5, 25°C
native recombinant enzyme
pH 5.0, 25°C


organism: Rhodococcus jostii

smiles: OO


enzyme_display_name: dye decolorizing peroxidase

substrate_name: H2O2; Reactive blue 5

proof_lines: pH and temperature not specified in the publication

enzyme: dye decolorizing peroxidase

substrate: H2O2; Reactive blue 5


organism: Auricularia auricula-judae


enzyme_display_name: acetate kinase

organism: Methanosarcina thermophila

substrate_name: ATP

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

enzyme: acetate kinase

substrate: ATP


substrate_name: veratrylglycerol-beta-guaiacyl ether

proof_lines:
at pH 3.0 and 25°C
Raw SKiD Env: Temp=25°C, pH=3
Raw SKiD Env: Temp=25°C, pH=3.5

substrate: veratrylglycerol-beta-guaiacyl ether


substrate_name: Reactive Blue 19

substrate: Reactive Blue 19


organism: Dictyostelium discoideum

substrate_name: pyrogallol

substrate: pyrogallol


substrate_name: Reactive Blue 4

substrate: Reactive Blue 4


organism: Irpex lacteus


assay_conditions_summary: pH 7; 310.15 K; cofactor ATP

proof_lines: pH 7.0, 37°C, mutant H180D, produced in E. coli


substrate_name: acetate

proof_lines: pH 7.0, 37°C, mutant H90A, produced in E. coli

substrate: acetate


proof_lines: pH 7.0, 37°C, mutant H180K, produced in E. coli


proof_lines: pH 7.0, 37°C, mutant H180E, produced in E. coli


substrate_name: manganese

substrate: manganese


substrate_name: 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) (Dye-decolorizing peroxidase AauDyP1, at pH 4.5)

assay_conditions_summary: pH 3


organism: Exidia glandulosa


organism: Mycena epipterygia


organism: Mycetinis scorodonius


enzyme_display_name: catalase peroxidase

organism: Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)

substrate_name: H(2)O(2) for the catalase reaction (at pH 5.5-6.0)

enzyme: catalase peroxidase


organism: Burkholderia pseudomallei (strain K96243)

substrate: H(2)O(2) for the catalase reaction (at pH 5.5-6.0)


organism: Escherichia coli (strain K12)


organism: Geobacillus stearothermophilus


organism: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

substrate_name: H(2)O(2) for the catalase reaction

assay_conditions_summary: cofactor NADPH

substrate: H(2)O(2) for the catalase reaction


organism: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)


enzyme_display_name: alpha-galactosidase

organism: Homo sapiens

proof_lines: mutant enzyme M296V, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C

enzyme: alpha-galactosidase


proof_lines: mutant enzyme A156V, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


proof_lines: mutant enzyme M72V, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


proof_lines: mutant enzyme L166V, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


organism: Medicago sativa subsp. varia

substrate_name: pNP-beta-D-xlyoside (at 37 degrees Celsius)


organism: Streptomyces lividans

substrate_name: p-nitrophenyl alpha-L-arabinofuranoside (pNP-Araf) (at 60 degrees Celsius and at pH 6)

assay_conditions_summary: pH 6


substrate_name: wheat arabinoxylan (at 55 degrees Celsius)


organism: Aspergillus oryzae (strain ATCC 42149 / RIB 40)

substrate_name: birch wood xylan


organism: Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936)

substrate_name: Unknown

substrate: Unknown


organism: Humicola insolens

substrate_name: birchwood xylan

substrate: birchwood xylan


substrate_name: beechwood xylan

substrate: beechwood xylan


organism: Paenibacillus sp. (strain JDR-2)


organism: Streptomyces sp


substrate_name: 2-methoxy-5-methyl-1,4-benzoquinone (reaction performed in 20 mM Tris (pH 7.0))

assay_conditions_summary: pH 7; buffer condition variant; cofactor NADH


enzyme_display_name: flavin-containing monooxygenase

substrate_name: trimethylamine (at pH 8.5)

enzyme: flavin-containing monooxygenase

substrate: trimethylamine (at pH 8.5)


organism: Macaca mulatta

substrate_name: N,N-dimethylaniline

substrate: N,N-dimethylaniline


organism: Oryctolagus cuniculus

substrate_name: thiourea

substrate: thiourea


organism: Rattus norvegicus


enzyme_display_name: formate dehydrogenase

organism: Arabidopsis thaliana

substrate_name: formate(at pH 7 and 30 degrees Celsius)

enzyme: formate dehydrogenase

substrate: formate(at pH 7 and 30 degrees Celsius)


organism: Candida boidinii

substrate_name: formate (at 30 degrees Celsius and at pH 7.5)

assay_conditions_summary: pH 7.5; buffer GF


enzyme_display_name: (3R)-2'-hydroxyisoflavanone reductase

organism: Medicago sativa

substrate_name: vestitone

enzyme: (3R)-2'-hydroxyisoflavanone reductase

substrate: vestitone


enzyme_display_name: citrate synthase (unknown stereospecificity)

organism: Aspergillus fumigatus (strain CBS 144.89 / FGSC A1163 / CEA10)

substrate_name: propionyl-CoA

substrate: propionyl-CoA


organism: Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)

substrate_name: propionyl-CoA (in the presence of 1 mM oxaloacetate)

enzyme: citrate synthase (unknown stereospecificity)


organism: Escherichia coli (strain K12)

substrate_name: oxaloacetate (at pH 8 and 35 degrees Celsius)

assay_conditions_summary: pH 8; cofactor CoA


organism: Fusarium solani


organism: Gibberella moniliformis


organism: Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)

substrate_name: acetyl-CoA

substrate: acetyl-CoA


organism: Yarrowia lipolytica (strain CLIB 122 / E 150)


enzyme_display_name: thymidine kinase

organism: Dictyostelium discoideum

substrate_name: thymidine

enzyme: thymidine kinase

substrate: thymidine


organism: Homo sapiens


organism: Mus musculus


organism: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

substrate_name: ATP

assay_conditions_summary: cofactor ATP

substrate: ATP


organism: Vaccinia virus (strain Western Reserve)


enzyme_display_name: aryl sulfotransferase

organism: Bos taurus

enzyme: aryl sulfotransferase


organism: Canis lupus familiaris


organism: Gallus gallus


organism: Amycolatopsis sp. (strain ATCC 39116 / 75iv2)

substrate_name: ABTS

proof_lines: kcat is 87 sec(-1) for peroxidase activity with ABTS as substrate. kcat is 34 sec(-1) for peroxidase activity with Reactive Blue 5 as substrate. kcat is 0.38 sec(-1) for peroxidase activity with Reactive Black 5 as substrate. kcat is 68 sec

substrate: ABTS


organism: Thermobifida fusca (strain YX)

substrate_name: Reactive Blue 19

proof_lines: kcat is 10 sec(-1) with Reactive Blue 19 as substrate.

substrate: Reactive Blue 19


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: H(2)O(2) in the catalase reaction (at pH 7.0)

proof_lines: kcat is 10100 sec(-1) for the catalase reaction (at pH 7.0 and 25 degrees Celsius).


organism: Pyricularia oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)

substrate_name: H(2)O(2) for the catalase reaction

proof_lines: kcat is 7010 sec(-1) with H(2)O(2) as substrate.


substrate_name: H(2)O(2) for the catalase reaction (at pH 5.25)

proof_lines: kcat is 6446 sec(-1) with H(2)O(2) as substrate at pH 5.25 and 3290 sec(-1) with H(2)O(2) as substrate at pH 7.0.


substrate_name: NADPH

proof_lines: kcat is 11.84 min(-1) for the NADPH oxidase activity releasing hydrogen peroxide from O2.

substrate: NADPH


proof_lines: kcat is 55 min(-1) for the NAPDPH-dependent oxygenation of hypotaurine (at pH 8.5 and 37 degrees Celsius).


organism: Pseudomonas sp. (strain 101)

substrate_name: formate

proof_lines: kcat is 10 sec(-1) with formate as substrate.

substrate: formate


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

proof_lines: kcat is 6.5 sec(-1) with NAD(+) as substrate.


organism: Thermochaetoides thermophila (strain DSM 1495 / CBS 144.50 / DAOM 24625 / IMI 039719)

substrate_name: formate (at pH 5.0 and 25 degrees Celsius)

assay_conditions_summary: pH 5

proof_lines: kcat is 0.8 sec(-1) with formate as substrate.


enzyme_display_name: thymidylate synthase

organism: Escherichia coli (strain K12)

substrate_name: dUMP

enzyme: thymidylate synthase

substrate: dUMP


enzyme_display_name: beta-fructofuranosidase

organism: Schwanniomyces occidentalis

substrate_name: sucrose

enzyme: beta-fructofuranosidase

substrate: sucrose


organism: Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372)

substrate_name: wheat arabinoxylan (at 37 degrees Celsius and at pH 7)


organism: Arabidopsis thaliana

substrate_name: p-nitrophenyl-beta-D-xylopyranoside

verification_status: corrected

substrate: p-nitrophenyl-beta-D-xylopyranoside

status: corrected


substrate_name: p-nitrophenyl-beta-D-xylopyranoside (at 37 degrees Celsius)


organism: Aspergillus kawachii (strain NBRC 4308)

substrate_name: L-arabinofuranose

substrate: L-arabinofuranose


organism: Bacillus subtilis (strain 168)

substrate_name: sugar beet arabinan (branched)

substrate: sugar beet arabinan (branched)


organism: Bifidobacterium longum

substrate_name: p-nitrophenyl alpha-L-arabinofuranoside (pNP-Araf)(at pH 6 and 45 degrees Celsius)

assay_conditions_summary: pH 6; 318.15 K; buffer 0.5 ml (total volume) of 50 mM potassium phosphate


organism: Humicola insolens

substrate_name: pNPX

substrate: pNPX


substrate_name: pNPAf

substrate: pNPAf


enzyme_display_name: aspartate transaminase

substrate_name: L-aspartate

proof_lines: kcat is 217 sec(-1) for the forward reaction. kcat is 574 sec(-1) for the reverse reaction.

enzyme: aspartate transaminase

substrate: L-aspartate


organism: Cereibacter sphaeroides (strain ATCC 17029 / ATH 2.4.9)

substrate_name: oxaloacetate

proof_lines: kcat is 150 sec(-1) with oxaloacetate as substrate. kcat is 56 sec(-1) with prephenate as substrate.

substrate: oxaloacetate


organism: Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)

proof_lines: kcat is 17.2 sec(-1) with 2-oxoglutarate as substrate. kcat is 12.3 sec(-1) with prephenate as substrate.


substrate_name: aspartate

proof_lines: kcat is 136 sec(-1) with aspartate as substrate. kcat is 198 sec(-1) with kynurenine as substrate.

substrate: aspartate


organism: Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)

proof_lines: kcat is 33 sec(-1) with oxaloacetate as substrate. kcat is 0.8 sec(-1) with prephenate as substrate.


organism: Rhizobium meliloti (strain 1021)

proof_lines: kcat is 205 sec(-1) with oxaloacetate as substrate. kcat is 43 sec(-1) with prephenate as substrate.


organism: Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

proof_lines: kcat is 54.2 sec(-1) with 2-oxoglutarate as substrate. kcat is 1.1 sec(-1) with prephenate as substrate.


organism: Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

substrate_name: L-aspartate (at 45 degrees Celsius)

proof_lines: kcat is 7.7 sec(-1) toward prephenate. kcat is 32.2 sec(-1) toward oxaloacetate.

substrate: L-aspartate (at 45 degrees Celsius)


substrate_name: deoxyuridine

proof_lines: kcat is 1.5 sec(-1) with deoxyuridine (dU) as substrate. kcat is 0.48 sec(-1) with deoxythymidine (dT) as substrate. kcat is 0.13 sec(-1) with 3'-azido-3'-deoxythymidine (AZT) as substrate.

substrate: deoxyuridine


proof_lines: kcat is 0.045 sec(-1) with deoxyuridine (dU) as substrate. kcat is 0.02 sec(-1) with deoxythymidine (dT) as substrate. kcat is 0.009 sec(-1) with 3'-azido-3'-deoxythymidine (AZT) as substrate.


organism: Homo sapiens

substrate_name: thymidine

proof_lines: Kcats are 9.5 sec(-1) and 3.5 sec(-1) with thymidine and AZT as substrates, respectively (PubMed:22385435). Kcat is 282 min(-1) with thymidine (PubMed:14697231).

substrate: thymidine


organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

substrate_name: acetate

substrate: acetate


organism: Rhodobacter capsulatus

substrate_name: H(2)O(2) for the catalase reaction (at pH 5.5-6.0)


organism: Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805)

substrate_name: H(2)O(2) for catalase activity

substrate: H(2)O(2) for catalase activity


substrate: H(2)O(2) for the catalase reaction (at pH 5.5-6.0)


enzyme_display_name: unspecific peroxygenase

organism: Cyclocybe aegerita

substrate_name: ABTS (at pH 4.5)

enzyme: unspecific peroxygenase

substrate: ABTS (at pH 4.5)


enzyme_display_name: tetracenomycin-F1 monooxygenase

organism: Streptomyces glaucescens

substrate_name: tetracenomycin F1

enzyme: tetracenomycin-F1 monooxygenase

substrate: tetracenomycin F1


enzyme_display_name: procollagen-proline 4-dioxygenase

organism: Arabidopsis thaliana

enzyme: procollagen-proline 4-dioxygenase


organism: Mus musculus


substrate_name: oxaloacetate (with propionyl-CoA)

proof_lines: kcat is 1.4 sec(-1) for citrate synthase activity with acetyl-CoA as substrate. kcat is 1.87 sec(-1) for citrate synthase activity with acetyl-CoA as substrate (at pH 8 and 30 degrees Celsius). kcat is 7.4 sec(-1) for 2-methylcitrate syntha

substrate: oxaloacetate (with propionyl-CoA)


organism: Thermosulfidibacter takaii (strain DSM 17441 / JCM 13301 / NBRC 103674 / ABI70S6)

substrate_name: citrate

assay_conditions_summary: cofactor CoA

proof_lines: kcat is 0.931 sec(-1) with citrate as substrate. kcat is 1.34 sec(-1) with CoA as substrate. kcat is 250 sec(-1) with acetyl-CoA as substrate.

substrate: citrate


enzyme_display_name: hypoxanthine phosphoribosyltransferase

organism: Dictyostelium discoideum

substrate_name: hypoxanthine

proof_lines: When guanine is used as the substrate, the rate of nucleotide formation is 50% that with hypoxanthine as the substrate.

enzyme: hypoxanthine phosphoribosyltransferase

substrate: hypoxanthine


organism: Escherichia coli (strain K12)

substrate_name: hypoxanthine (at pH 8.5 and 25 degrees Celsius)

proof_lines: kcat is 59.0 sec(-1) with hypoxanthine as substrate. kcat is 10.2 sec(-1) with guanine as substrate. kcat is 0.008 sec(-1) with xanthine as substrate (at pH 8.5 and 25 degrees Celsius).


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: guanine (at pH 7.4 and 25 degrees Celsius)

proof_lines: kcat is 0.14 sec(-1) with guanine as substrate. kcat is 0.9 sec(-1) with hypoxanthine as substrate (at pH 7.4 and 25 degrees Celsius) (PubMed:19362594). kcat is 0.62 sec(-1) with guanine as substrate. kcat is 0.55 sec(-1) with hypoxanthine


organism: Petunia hybrida

substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


enzyme_display_name: cysteine synthase

proof_lines: kcat is 120 min(-1) for O(3)-acetyl-L-serine for the cysteine synthase activity, kcat is 90 min(-1) for Na(2)S for the cysteine synthase activity, kcat is 160 min(-1) for cysteine for the L-3-cyanoalanine synthase activity, kcat is 130 min(

enzyme: cysteine synthase


organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843)

substrate_name: O-succinyl-L-serine

proof_lines: kcat is 2.8 sec(-1) with O-succinyl-L-serine as substrate. kcat is 1.5 sec(-1) with O-acetyl-L-serine as substrate.

substrate: O-succinyl-L-serine


enzyme_display_name: protein farnesyltransferase

substrate_name: CVIM substrate

proof_lines: kcat is 49.1 h(-1), 28.8 h(-1), 12.8 h(-1) and 3.7 h(-1) for CVIQ, CVIM, CVII and CVIL substrates, respectively.

enzyme: protein farnesyltransferase

substrate: CVIM substrate


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: farnesyl diphosphate

proof_lines: kcat is 4.5 sec(-1) with dansyl-GCVIA as substrate.

substrate: farnesyl diphosphate


substrate_name: L-aspartate

proof_lines: kcat is 176 sec(-1) for the forward reaction. kcat is 279 sec(-1) for the reverse reaction.

substrate: L-aspartate


proof_lines: kcat is 205 sec(-1) for the forward reaction. kcat is 319 sec(-1) for the reverse reaction.


enzyme_display_name: acetate kinase

enzyme: acetate kinase


enzyme_display_name: adenylate kinase

substrate_name: AMP

proof_lines: kcat is 0.0063 sec(-1) for adenylate kinase activity. kcat is 0.00048 sec(-1) for ATPase activity.

enzyme: adenylate kinase

substrate: AMP


organism: Plasmodium falciparum (isolate 3D7)

substrate_name: AMP (at 25 degrees Celsius and pH 6 or pH 7.4)

assay_conditions_summary: pH 6; cofactor ATP

proof_lines: kcat is 35 sec(-1) (at 25 degrees Celsius and pH 6).


substrate_name: AMP (at 25 degrees Celsius and pH 7.6)

assay_conditions_summary: pH 7.6; cofactor ATP

proof_lines: kcat is 5.4 sec(-1) (at 25 degrees Celsius and pH 7.6).

substrate: AMP (at 25 degrees Celsius and pH 7.6)


substrate_name: ATP (for adenylate kinase activity)

assay_conditions_summary: buffer or cofactor; cofactor ATP

proof_lines: kcat is 0.00612 sec(-1) for adenylate kinase activity.

substrate: ATP (for adenylate kinase activity)


organism: Schistosoma mansoni

substrate_name: AMP (at pH 7.4)

assay_conditions_summary: pH 7.4; cofactor ATP

proof_lines: kcat is 8.3 sec(-1) with AMP as substrate. kcat is 5.0 sec(-1) with ATP as substrate.

substrate: AMP (at pH 7.4)


proof_lines: kcat is 50 sec(-1) with AMP as substrate. kcat is 25 sec(-1) with ATP as substrate.


organism: Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

substrate_name: nicotinamide mononucleotide

proof_lines: kcat is 4.9 sec(-1) with nicotinamide mononucleotide as substrate. kcat is 3.0 sec(-1) with nicotinate mononucleotide as substrate.

substrate: nicotinamide mononucleotide


organism: Homo sapiens

substrate_name: NMN

proof_lines: KM is >100 uM for triazofurin monophosphate.

substrate: NMN


substrate_name: nicotinamide mononucleotide (NMN)

proof_lines: kcat is 4.5 sec(-1) with ATP as substrate (PubMed:19403820). kcat is 5.5 sec(-1) with nicotinamide mononucleotide (NMN) as substrate (PubMed:19403820).

substrate: nicotinamide mononucleotide (NMN)


organism: Caenorhabditis elegans

substrate_name: bisphenol A (at 25 degrees Celsius and pH 6.8)

proof_lines: kcat is 0.283 sec(-1) with bisphenol A as substrate (at 25 degrees Celsius and pH 6.8) (PubMed:16567400). kcat is 0.909 sec(-1) with 3'-phosphoadenylyl sulfate (PAPS) as substrate (at 25 degrees Celsius and pH 6.8, and with 4-hydroxyphenyla


organism: Mycoplasmopsis agalactiae (strain NCTC 10123 / CIP 59.7 / PG2)

proof_lines: kcat is 51 sec(-1) with D-xylono-1,4-lactone-5-phosphate as substrate. kcat is 8.7 sec(-1) with L-arabino-1,4-lactone-5-phosphate as substrate.


organism: Methanosarcina thermophila

substrate_name: acetate

substrate: acetate


substrate_name: ATP

substrate: ATP


enzyme_display_name: alpha-glucosidase

organism: Culex quinquefasciatus

substrate_name: sucrose

proof_lines: kcat is 45.0 min(-1) with sucrose as substrate (PubMed:38428508). kcat is 329 sec(-1) with sucrose as substrate (PubMed:25746591). kcat is 84 sec(-1) with maltose as substrate (PubMed:25746591). kcat is 320 sec(-1) with maltotriose as subst

enzyme: alpha-glucosidase

substrate: sucrose


enzyme_display_name: alpha-galactosidase

organism: Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)

substrate_name: p-nitrophenyl alpha-galactoside (at pH 6.0 and 37 degrees Celsius)

proof_lines: kcat is 244 sec(-1) with p-nitrophenyl alpha-galactoside as substrate (at pH 6.0 and 37 degrees Celsius).

enzyme: alpha-galactosidase


organism: Geobacillus stearothermophilus

proof_lines: kcat is 105 sec(-1) for p-nitrophenyl-alpha-galactopyranoside (at 25 degrees Celsius and pH 6.5). kcat is 180 sec(-1) for raffinose (at 25 degrees Celsius and pH 6.5).


proof_lines: kcat is 0.047 min-1 with the disaccharide Gal-alpha1,4-Glc as substrate.


organism: Mediterraneibacter gnavus

substrate_name: p-nitrophenyl-alpha-D-galactopyranoside (at pH 6.0 and 37 degrees Celsius)

proof_lines: kcat/KM is 1850 s(1) mM(1) for p-nitrophenyl-alpha-D-galactopyranoside.


organism: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

proof_lines: kcat is 176 s(1) for p-nitrophenol-alpha-galactoside (at pH 5.0 and 75 degrees Celsius). kcat is 109 s(1) for raffinose (at pH 5.0 and 75 degrees Celsius) (PubMed:9741105). kcat is 8 s(1) for p-nitrophenyl-alpha-D-galactopyranoside (at pH 5


organism: Bacillus subtilis (strain 168)

substrate_name: linear alpha-1,5-L-arabinan (at pH 6.6 and 37 degrees Celsius)

proof_lines: kcat is 12 sec(-1) with linear alpha-1,5-L-arabinan as substrate (PubMed:18757805). kcat is 306 sec(-1) with pNP-Araf as substrate (PubMed:18757805). kcat is 32 sec(-1) with arabinobiose as substrate (PubMed:18757805). kcat is 88 sec(-1) wi


organism: Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153)

substrate_name: L- Araf-alpha-PNP

proof_lines: kcat is 0.0005 sec(-1) for L- Araf-alpha-PNP.

substrate: L- Araf-alpha-PNP


substrate_name: L-Araf-alpha-PNP

proof_lines: kcat is 0.057 sec(-1) for L-Araf-alpha-PNP. kcat is 0.26 sec(-1) for Xyl-beta-PNP.

substrate: L-Araf-alpha-PNP


organism: Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)

substrate_name: larch arabinogalactan

proof_lines: kcat is 220 sec(-1) with larch arabinogalactan as substrate.

substrate: larch arabinogalactan


organism: Cellvibrio japonicus (strain Ueda107)

substrate_name: arabinan

proof_lines: kcat is 17.1 sec(-1) for arabinan. kcat is 212 sec(-1) for carboxymethylarabinan.

substrate: arabinan


proof_lines: Apparently, AbfA can accommodate xylopyranose in the active site, but without the necessary distortion required for the efficient catalysis of six-membered rings. This explains in part its lower specificity towards the xylopyranosidic subst


organism: Pyricularia oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)

proof_lines: kcat is 0.9148 sec(-1) with p-nitrophenyl-alpha-1-arabinofuranoside as substrate.


organism: Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)

substrate_name: p-nitrophenyl-alpha-L-arabinofuranoside

proof_lines: kcat is 6.5 min(-1) with p-nitrophenyl-alpha-L-arabinofuranoside as substrate.

substrate: p-nitrophenyl-alpha-L-arabinofuranoside


organism: Xylanibacter ruminicola (strain ATCC 19189 / DSM 19721 / CIP 105475 / JCM 8958 / 23)

substrate_name: pNP-beta-D-xylopyranoside (pNPX) (at 37 degrees Celsius and pH 5.0)

proof_lines: kcat is 9.7 sec(-1) for the beta-xylosidase activity with pNPX as substrate (at 37 degrees Celsius and pH 5.0).


organism: Botrytis cinerea (strain B05.10)

substrate_name: low viscosity xylan

substrate: low viscosity xylan


organism: Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)

substrate_name: wheat flour arabinoxylan

substrate: wheat flour arabinoxylan


substrate_name: xylan

substrate: xylan


organism: Hypocrea rufa

substrate_name: xylan at 20 degrees Celsius

substrate: xylan at 20 degrees Celsius


organism: Neocallimastix patriciarum

substrate_name: birchwood xylan for endo-1,4-beta-xylanase activity


substrate_name: oat spelt xylan

substrate: oat spelt xylan


organism: Paenibacillus barcinonensis

substrate_name: beechwood xylan

substrate: beechwood xylan


organism: Methanosarcina thermophila

substrate_name: ATP


substrate_name: acetate


enzyme_display_name: subtilisin

organism: Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)

proof_lines: kcat is 1.6 sec(-1) for N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide at 20 degrees Celsius and pH 7.5 (PubMed:20100702). kcat is 25 sec(-1) for N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide at 80 degrees Celsius and pH 7.5 (PubMed:20100702).

enzyme: subtilisin


enzyme_display_name: flavivirin

organism: Dengue virus type 2 (strain Thailand/NGS-C/1944)

substrate_name: triphosphorylated RNA

proof_lines: Vmax with 0.65 nmol/s/ug enzyme for RTPase activity with triphosphorylated RNA as substrate.

enzyme: flavivirin

substrate: triphosphorylated RNA


enzyme_display_name: penicillin amidase

organism: Lysinibacillus sphaericus

substrate_name: penicillin V

enzyme: penicillin amidase

substrate: penicillin V


enzyme_display_name: arginase

organism: Arabidopsis thaliana

substrate_name: agmatine

smiles: C(CCN=C(N)N)CN

proof_lines: kcat is 6.28 min(-1) for the hydrolysis of L-arginine in presence of dioleoyl phosphatidic acid (at 37 degrees Celsius and pH 9.5) (PubMed:35752016). kcat is 5.44 min(-1) for the hydrolysis of L-arginine in presence of phosphatidylcholine (

enzyme: arginase

substrate: agmatine


organism: Bacillus caldovelox

proof_lines: kcat is 700 sec(-1) for the hydrolysis of L-arginine (at pH 9.5 and at 55 degrees Celsius).


organism: Oryza sativa subsp. indica

substrate_name: L-arginine

proof_lines: kcat is 490 sec(-1) with L-arginine as substrate.

substrate: L-arginine


organism: Plasmodium falciparum (isolate 3D7)

substrate_name: L-arginine (at pH 8 and 37 degrees Celsius)

assay_conditions_summary: pH 8

proof_lines: kcat is 440 sec(-1) with L-arginine as substrate (at pH 8.5 and 37 degrees Celsius) (PubMed:20527960). kcat is 96 sec(-1) with L-arginine as substrate (at pH 8 and 37 degrees Celsius) (PubMed:15843155). kcat is 320 sec(-1) with L-arginine a


organism: Schistosoma mansoni

proof_lines: kcat is 537 sec(-1) for the hydrolysis of L-arginine (at pH 9.7 and at 37 degrees Celsius) (PubMed:18723022). kcat is 330 sec(-1) for the hydrolysis of L-arginine (at pH 8.5) (PubMed:25007099).


enzyme_display_name: orotidine-5'-phosphate decarboxylase

organism: Homo sapiens

substrate_name: orotidine 5'-phosphate

proof_lines: kcat is 0.75 sec(-1) with orotidine 5'-phosphate as substrate.

enzyme: orotidine-5'-phosphate decarboxylase

substrate: orotidine 5'-phosphate


enzyme_display_name: phosphoenolpyruvate carboxykinase (GTP)

substrate_name: phosphoenolpyruvate

proof_lines: kcat is 32 sec(-1) with oxaloacetate as substrate. kcat is 585 sec(-1) with phosphoenolpyruvate as substrate.

enzyme: phosphoenolpyruvate carboxykinase (GTP)

substrate: phosphoenolpyruvate


substrate_name: oxaloacetate (for the enzyme purified from cells exposed to 10 mM glucose)

proof_lines: kcat is 33 sec(-1) with oxaloacetate as substrate (for the enzyme purified from cells exposed to 10 mM glucose) (PubMed:30193097). kcat is 21 sec(-1) with oxaloacetate as substrate (for the enzyme purified from cells exposed to 15 mM glucos


organism: Rattus norvegicus

substrate_name: oxaloacetate

proof_lines: kcat is 76 sec(-1) with oxaloacetate as substrate (PubMed:30193097). kcat is 27 sec(-1) with phosphoenolpyruvate as substrate (PubMed:30193097). kcat is 65 sec(-1) with GTP as substrate (PubMed:30193097). kcat is 25 sec(-1) with GDP as subs

substrate: oxaloacetate


organism: Penicillium oxalicum

substrate_name: birchwood xylan

substrate: birchwood xylan


organism: Phanerodontia chrysosporium


organism: Rhizopus oryzae


organism: Talaromyces funiculosus


organism: Trichoderma reesei (strain ATCC 56765 / BCRC 32924 / NRRL 11460 / VTT-D-86271 / Rut C-30)

substrate_name: beechwood (unsubstituted) xylan

proof_lines: kcat is 68 sec(-1) with xylohexaose, 50.3 sec(-1) with xylopentaose, 0.162 sec(-1) with xylotetraose and 0.045 sec(-1) with xylotriose as substrate.

substrate: beechwood (unsubstituted) xylan


enzyme_display_name: peptidyl-dipeptidase A

substrate_name: Hip-His-Leu

proof_lines: kcat is 500 min(-1) for angiotensin I (PubMed:6270633). kcat is 500 min(-1) for bradykinin (PubMed:6270633). kcat is 3500 min(-1) for Met-enkephalin (PubMed:6270633). kcat is 700 min(-1) for Leu-enkephalin (PubMed:6270633). kcat is 12 sec(-

enzyme: peptidyl-dipeptidase A

substrate: Hip-His-Leu


organism: Bacillus licheniformis

proof_lines: kcat is 1.6 sec(-1) with Suc-Ala(2)-Ala-pNA as substrate. kcat is 57 sec(-1) with Suc-Ala(2)-Phe-pNA as substrate. kcat is 97 sec(-1) with MeO-Suc-Ala(2)-Phe-pNA as substrate (Ref.4). kcat is 0.990 sec(-1) with Suc-Gly(2)-Phe-pNA as substra


organism: Methanosarcina thermophila

assay_conditions_summary: pH 7; 293.15 K; cofactor ATP

proof_lines: pH 7.0, 20°C, mutant E97Q, produced in E. coli

substrate: acetate


proof_lines: pH 7.0, 20°C, mutant E385Q, produced in E. coli

substrate: ATP


proof_lines: pH 7.0, 20°C, mutant E385D, produced in E. coli


proof_lines: pH 7.0, 20°C, mutant E385A, produced in E. coli


enzyme_display_name: glycine hydroxymethyltransferase

organism: Escherichia coli

substrate_name: tetrahydrofolate

enzyme: glycine hydroxymethyltransferase

substrate: tetrahydrofolate


proof_lines: pH 7.0, 20°C, mutant E334A, produced in E. coli


proof_lines: pH 7.0, 20°C, mutants E97A, E97D, E97Q, produced in E. coli


proof_lines: pH 7.0, 20°C, mutant E385C, produced in E. coli


proof_lines: pH 7.0, 20°C, mutant E97D, produced in E. coli


proof_lines: wild-type enzyme, pH and temperature not specified in the publication


substrate_name: L-Ser

substrate: L-Ser


organism: Sus scrofa

proof_lines: kcat is 6.8 sec(-1) with phosphoenolpyruvate as substrate (PubMed:26792594). kcat is 7.6 sec(-1) with GDP as substrate (PubMed:26792594). kcat is 46 sec(-1) with oxaloacetate as substrate. kcat is 39 sec(-1) with GTP as substrate (PubMed:26


enzyme_display_name: (S)-hydroxynitrile lyase

organism: Hevea brasiliensis

smiles: CC(C)(C#N)O

proof_lines: The unusual high KM of HbHNL is consistent with the concentration of cyanoglucosides in young leaves of Hevea brasiliensis.

enzyme: (S)-hydroxynitrile lyase


organism: Manihot esculenta

proof_lines: The large KM for 2-hydroxy-2-methylpropanenitrile is not inconsistent with the concentration of cyanoglucosides in cassava leaves.


enzyme_display_name: tryptophan synthase

organism: Zea mays

substrate_name: indole-3-glycerol phosphate

proof_lines: kcat is 0.02 sec(-1) with indole-3-glycerol phosphate as substrate.

enzyme: tryptophan synthase

substrate: indole-3-glycerol phosphate


proof_lines: pH 7.0, 20°C, mutant E97A, produced in E. coli


substrate_name: ATP

proof_lines: pH 7.0, 20°C, mutant E32A, produced in E. coli


proof_lines: pH 7.0, 20°C, unaltered enzyme, produced in E. coli


enzyme_display_name: thymidine kinase

substrate_name: thymidine

assay_conditions_summary: pH 7.5; cofactor ATP

proof_lines: pH 7.5, recombinant mutant V106A, in absence of ATP

enzyme: thymidine kinase

substrate: thymidine


proof_lines: pH 7.5, recombinant mutant V106H, in absence of ATP


proof_lines: pH 7.5, recombinant mutants V106L and V106G, in absence of ATP


proof_lines: pH 7.5, recombinant mutant V106Q, in absence or presence of ATP


proof_lines: pH 7.5, recombinant mutants V106L, V106H and V106T, in presence of ATP


proof_lines: pH 7.5, recombinant mutant V106M, in absence or presence of ATP


proof_lines: pH 7.5, recombinant mutant V106I, in absence of ATP

proof_lines: pH 7.5, recombinant mutant V106I, in presence of ATP


proof_lines: pH 7.5, recombinant mutant V106K, in absence of ATP

proof_lines: pH 7.5, recombinant mutant V106K, in presence of ATP


proof_lines: pH 7.5, recombinant mutant V106T, in absence of ATP


proof_lines: pH 7.5, recombinant mutants V106A and V106G, in presence of ATP


substrate_name: acetate

assay_conditions_summary: pH 7.6; 310.15 K; cofactor ATP


assay_conditions_summary: pH 7; 310.15 K; cofactor ATP

proof_lines: pH 7.0, 37°C, mutant H94A, produced in E. coli


proof_lines: pH 7.0, 37°C, mutant H180N, produced in E. coli


proof_lines: pH 7.0, 37°C, mutant H60A, produced in E. coli


proof_lines: pH 7.0, 37°C, mutant H180E, H180N, produced in E. coli


organism: Penicillium griseofulvum

substrate_name: wheat arabinoxylan

proof_lines: mutant S44A, calculated as xylose units released from wheat arabinoxylan

substrate: wheat arabinoxylan


proof_lines: mutant S44D, calculated as xylose units released from wheat arabinoxylan


proof_lines: mutant S129G/S44N, calculated as xylose units released from wheat arabinoxylan


mutation_type: deletion

proof_lines: mutant DELTAD130, calculated as xylose units released from wheat arabinoxylan


proof_lines: mutant Q121R, calculated as xylose units released from wheat arabinoxylan


proof_lines: mutant S44N, calculated as xylose units released from wheat arabinoxylan


proof_lines: pH 7.0, 37°C, mutant H123A, produced in E. coli


proof_lines: pH 7.0, 37°C, mutant H184A, produced in E. coli


proof_lines: pH 7.0, 37°C, mutant H180Q, produced in E. coli


proof_lines: pH 7.0, 37°C, mutant H180R, produced in E. coli


proof_lines: pH 7.0, 37°C, mutant H180A, produced in E. coli


proof_lines: pH 7.0, 37°C, mutant H208A, produced in E. coli


enzyme_display_name: citrate synthase (unknown stereospecificity)

substrate_name: acetyl-CoA

assay_conditions_summary: cofactor NADH

proof_lines: mutant enzyme H110A, in presence of 0.1 M KCl

substrate: acetyl-CoA


proof_lines: mutant enzyme Y145A, in presence of 0.1 M KCl

enzyme: citrate synthase (unknown stereospecificity)


proof_lines: mutant enzyme R109L, in presence of 0.1 M KCl


proof_lines: mutant enzyme T204A, in presence of 0.1 M KCl


proof_lines: mutant enzyme N189A, in presence of 0.1 M KCl


proof_lines: mutant enzyme R163L, in presence of 0.1 M KCl


proof_lines: mutant enzyme Q182A, in presence of 0.1 M KCl


proof_lines: mutant enzyme T111A, in presence of 0.1 M KCl


proof_lines: mutant enzyme K167A, in presence of 0.1 M KCl


proof_lines: wild-type, calculated as xylose units released from wheat arabinoxylan


proof_lines: mutant F14Y, calculated as xylose units released from wheat arabinoxylan


proof_lines: mutant S129G, calculated as xylose units released from wheat arabinoxylan

proof_lines: mutant S129G/DELTAD130, calculated as xylose units released from wheat arabinoxylan


proof_lines: mutant R7T, calculated as xylose units released from wheat arabinoxylan


enzyme_display_name: beta-fructofuranosidase

organism: Schwanniomyces occidentalis

substrate_name: nystose

enzyme: beta-fructofuranosidase

substrate: nystose


substrate_name: inulin

substrate: inulin


enzyme_display_name: alkaline phosphatase

organism: Vibrio splendidus

substrate_name: 4-nitrophenyl phosphate

enzyme: alkaline phosphatase

substrate: 4-nitrophenyl phosphate


enzyme_display_name: sulfite oxidase

organism: Homo sapiens

substrate_name: sulfite

enzyme: sulfite oxidase

substrate: sulfite


enzyme_display_name: branched-chain-amino-acid transaminase

substrate_name: L-leucine

assay_conditions_summary: pH 7.8; 310.15 K; buffer 25 mM potassium phosphate

proof_lines: mutant enzyme C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C

enzyme: branched-chain-amino-acid transaminase

substrate: L-leucine


substrate_name: L-glutamate

proof_lines: mutant enzyme C293S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C

substrate: L-glutamate


proof_lines: mutant enzyme C221S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C


proof_lines: mutant enzyme C235S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C


enzyme_display_name: alpha-galactosidase

assay_conditions_summary: pH 4.6; 310.15 K; buffer 0.1 M sodium citrate

proof_lines: mutant enzyme R112H, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C

enzyme: alpha-galactosidase


proof_lines: mutant enzyme I91T, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


proof_lines: mutant enzyme M296I, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


proof_lines: mutant enzyme R301Q, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


proof_lines: 25°C, pH 8.25, 100 mM buffer, wild-type enzyme

proof_lines: 25°C, pH 8.0, 100 mM buffer, wild-type enzyme


proof_lines:
mutant V474M, 25°C, pH 7.0
mutant V474M, 25°C, pH 8.5
mutant V474M, 25°C, pH 10.0


proof_lines:
mutant R472Q, 25°C, pH 10.0
mutant R472Q, 25°C, pH 9.5
mutant R472Q, 25°C, pH 8.0


proof_lines:
mutant Y343N, 25°C, pH 8.5
mutant Y343N, 25°C, pH 9.5
mutant Y343N, 25°C, pH 9.0


enzyme_display_name: dihydrolipoyl dehydrogenase

organism: Leishmania donovani

substrate_name: dihydrolipoamide

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NAD+

enzyme: dihydrolipoyl dehydrogenase

substrate: dihydrolipoamide


substrate_name: NAD+

substrate: NAD+


proof_lines:
mutant Y343N/R472M, 25°C, pH 6.0
mutant Y343N/R472M, 25°C, pH 7.0
mutant Y343N/R472M, 25°C, pH 8.0


proof_lines:
mutant Y343F/R472Q, 25°C, pH 9.0
mutant Y343F/R472Q, 25°C, pH 7.0
mutant Y343F/R472Q, 25°C, pH 8.0


proof_lines:
mutant Y343N/R472Q, 25°C, pH 9.5
mutant Y343N/R472Q, 25°C, pH 6.0
mutant Y343N/R472Q, 25°C, pH 7.0


proof_lines:
mutant R472M, 25°C, pH 8.0
mutant R472M, 25°C, pH 8.5
mutant R472M, pH 7.6, 25°C


proof_lines: mutant enzyme C242S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C


substrate_name: glyoxylate

assay_conditions_summary: pH 7.4; 298.15 K; buffer 25 mM potassium phosphate

proof_lines: wild type enzyme, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C

substrate: glyoxylate


proof_lines: mutant enzyme C335S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C


proof_lines: mutant enzyme C335S/C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C


proof_lines: mutant enzyme E59K, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


proof_lines: mutant enzyme R356W, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


proof_lines: mutant enzyme Q279E, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


proof_lines: mutant enzyme F113L, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


substrate_name: beta-D-fructose 6-phosphate

mutation_signature: C-terminal deletion mutant L307

proof_lines: C-terminal deletion mutant L307, 1 mM co-substrate

substrate: beta-D-fructose 6-phosphate


proof_lines: Y306A, C-terminal point mutant, 1 mM co-substrate


mutation_signature: C-terminal deletion mutant A305

proof_lines: C-terminal deletion mutant A305, 1 mM co-substrate


mutation_signature: C-terminal deletion mutant Y306

proof_lines: C-terminal deletion mutant Y306, 1 mM co-substrate


proof_lines: mutant E322K, pH 8.0, 25°C, in presence of a phosphoryl group acceptor

proof_lines: mutant E322K, pH 8.0, 25°C, in absence of a phosphoryl group acceptor


proof_lines: mutant T155M, pH 8.0, 25°C, in absence of a phosphoryl group acceptor

proof_lines: mutant T155M, pH 8.0, 25°C, in presence of a phosphoryl group acceptor


proof_lines: mutant S105L, pH 8.0, 25°C, in absence of a phosphoryl group acceptor

proof_lines: mutant S105L, pH 8.0, 25°C, in presence of a phosphoryl group acceptor


proof_lines: mutant E341K, pH 8.0, 25°C, in presence of a phosphoryl group acceptor

proof_lines: mutant E341K, pH 8.0, 25°C, in absence of a phosphoryl group acceptor


enzyme_display_name: acetate kinase

assay_conditions_summary: pH 7; cofactor ATP

proof_lines:
mutant L122A, pH 7.0
mutant L122A, pH 7.4

enzyme: acetate kinase

substrate: ATP


substrate_name: propionate

proof_lines:
mutant P232A, pH 7.0
mutant P232A, pH 7.4

substrate: propionate


proof_lines:
mutant F179A, pH 7.0
mutant F179A, pH 7.4


proof_lines:
mutant V93G, pH 7.0
mutant V93G, pH 7.4


organism: Helicobacter pylori

substrate_name: L-arginine

proof_lines: mutant S88G/A92S, Mn2+-activated, pH 5.0, 37°C

proof_lines: mutant S88G/A92S, Co2+-activated, pH 5.0, 37°C, Hill coefficient 2.0

substrate: L-arginine


proof_lines: mutant H91A, Co2+-activated, pH 5.0, 37°C, Hill coefficient 2.3


mutation_signature: C-terminal deletion mutant D299

assay_conditions_summary: cofactor ATP

proof_lines: C-terminal deletion mutant D299, 1 mM co-substrate


proof_lines: L307A, C-terminal point mutant, 1 mM co-substrate


substrate_name: Butyrate

proof_lines:
wild-type, pH 7.0
wild-type, pH 7.5, 37°C
wild-type, pH 7.4

substrate: Butyrate


proof_lines:
mutant V93A, pH 7.0
mutant V93A, pH 7.4


proof_lines: mutant D434E, pH 8.0, 25°C, in absence of a phosphoryl group acceptor


proof_lines: mutant H412Y, pH 8.0, 25°C, in presence of a phosphoryl group acceptor

proof_lines: mutant H412Y, pH 8.0, 25°C, in absence of a phosphoryl group acceptor


proof_lines: mutant S89G, Co2+-activated, pH 5.0, 37°C

proof_lines: mutant S89G, Mn2+-activated, pH 5.0, 37°C, Hill coefficient 2.1


proof_lines: mutant E90A, Co2+-activated, pH 5.0, 37°C, Hill coefficient 1.7


proof_lines: mutant A92S, Mn2+-activated, pH 5.0, 37°C

proof_lines: mutant A92S, Co2+-activated, pH 5.0, 37°C, Hill coefficient 1.9


proof_lines: mutant S88G, Co2+-activated, pH 5.0, 37°C

proof_lines: mutant S88G, Mn2+-activated, pH 5.0, 37°C, Hill coefficient 2.4


enzyme_display_name: unspecific peroxygenase

organism: Thermothelomyces thermophilus

proof_lines: mutant enzyme F59Q/L60F/S159G, at pH 7.0 and 25°C

enzyme: unspecific peroxygenase


proof_lines: mutant enzyme F59Q/L60M/S159G/F154A, at pH 7.0 and 25°C


proof_lines: mutant enzyme L60F/S159G, at pH 7.0 and 25°C


assay_conditions_summary: cofactor NAD+


organism: Arthrobacter sp.

assay_conditions_summary: cofactor CoA


substrate_name: propionyl-CoA

substrate: propionyl-CoA


proof_lines:
mutant loop/K313L/A361R
mutant mutant loop/K313L/A361R


organism: Thermoplasma acidophilum

assay_conditions_summary: pH 8; 293.15 K; cofactor CoA

proof_lines:
wild-type, pH 8.0, 20°C
recombinant wild-type
recombinant wild-type, pH 8.0


substrate_name: citrate

substrate: citrate


substrate: acetyl-CoA


organism: Bacillus subtilis

substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


substrate_name: shikimate 3-phosphate

substrate: shikimate 3-phosphate


enzyme_display_name: arginase

organism: Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM)

substrate_name: L-arginine (at pH 7.5 and 37 degrees Celsius)

enzyme: arginase


organism: Glaciozyma antarctica (strain PI12)


organism: Rattus norvegicus

substrate_name: arginine

substrate: arginine


organism: Mycolicibacterium smegmatis

substrate_name: OAA (PEP forming activity at pH 7.2 and 37 degrees Celsius)


organism: Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)

substrate_name: oxaloacetate (at 60 degrees Celsius)

substrate: oxaloacetate (at 60 degrees Celsius)


organism: Escherichia coli


proof_lines:
recombinant wild-type enzyme
wild-type enzyme, 1 mM Mg2+
wild-type enzyme, 30 mM Mg2+


organism: Streptomyces sp

substrate_name: Unknown

substrate: Unknown


organism: Talaromyces funiculosus


organism: Thermomyces lanuginosus


organism: Drosophila melanogaster

substrate_name: angiotensin I

substrate: angiotensin I


substrate_name: Hip-His-Leu

substrate: Hip-His-Leu


organism: Oryctolagus cuniculus


enzyme_display_name: subtilisin

organism: Bacillus subtilis subsp. natto

substrate_name: Suc-Ala-Ala-Pro-Phe-pNA

enzyme: subtilisin

substrate: Suc-Ala-Ala-Pro-Phe-pNA


organism: Plasmodium falciparum

substrate_name: PEP1 (Ac-LVSADNIDIS) peptide

substrate: PEP1 (Ac-LVSADNIDIS) peptide


enzyme_display_name: flavivirin

organism: Dengue virus type 2 (strain Puerto Rico/PR159-S1/1969)

substrate_name: N-acetyl-Gly-Arg-Arg-AMC

enzyme: flavivirin

substrate: N-acetyl-Gly-Arg-Arg-AMC


organism: Kokobera virus

substrate_name: ATPase of serine protease NS3

substrate: ATPase of serine protease NS3


organism: Tick-borne encephalitis virus European subtype (strain Neudoerfl)

substrate_name: ATP

substrate: ATP


enzyme_display_name: penicillin amidase

organism: Bacillus subtilis (strain 168)

substrate_name: penicillin V

enzyme: penicillin amidase

substrate: penicillin V


organism: Caenorhabditis elegans

proof_lines: wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication


substrate_name: deoxycytidine

proof_lines: mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication

substrate: deoxycytidine


substrate_name: thymidine

proof_lines: mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication

substrate: thymidine


proof_lines: mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication


proof_lines: mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication


organism: Bambusa oldhamii

substrate_name: sucrose

proof_lines:
pH 5.0, 37°C, mutant enzyme C317A
pH 5.0, 37Â°C, mutant enzyme C317A

substrate: sucrose


proof_lines:
pH 5.0, 37°C, mutant enzyme W159L
pH 5.0, 37Â°C, mutant enzyme W159L


proof_lines:
pH 5.0, 37°C, mutant enzyme W159F
pH 5.0, 37Â°C, mutant enzyme W159F


proof_lines:
pH 5.0, 37°C, mutant enzyme E316Q
pH 5.0, 37Â°C, mutant enzyme E316Q


proof_lines:
pH 5.0, 37°C, wild-type enzyme
pH 5.0, 37Â°C, wild-type enzyme


enzyme_display_name: catalase peroxidase

organism: Mycobacterium tuberculosis

proof_lines: peroxidatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C

proof_lines: catalatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C

enzyme: catalase peroxidase


proof_lines: peroxidatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C

proof_lines: catalatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C


proof_lines: catalatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C

proof_lines: peroxidatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C


proof_lines: catalatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C

proof_lines: peroxidatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C


enzyme_display_name: thymidylate synthase

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: dUMP

enzyme: thymidylate synthase

substrate: dUMP


enzyme_display_name: glycine hydroxymethyltransferase

organism: Arabidopsis thaliana

substrate_name: H4PteGlu1 (at pH 8.5)

proof_lines: kcat is 15.8 sec(-1) with H4PteGlu1 as substrate (at pH 8.5) (PubMed:20518745). kcat is 7.6 sec(-1) with H4PteGlu2 as substrate (at pH 8.5) (PubMed:20518745). kcat is 8.7 sec(-1) with H4PteGlu3 as substrate (at pH 8.5) (PubMed:20518745). kc

enzyme: glycine hydroxymethyltransferase

substrate: H4PteGlu1 (at pH 8.5)


organism: Bombyx mori

substrate_name: L-serine (at pH 3.0 and 30 degrees Celsius)

assay_conditions_summary: pH 3

proof_lines: The kcat/Km for tetrahydrofolate is 0.081 mM(-1)sec(-1). The kcat/Km for L-serine is 0.0022 mM(-1)sec(-1).


organism: Escherichia coli (strain K12)

substrate_name: L-serine

proof_lines: kcat is 10.7 sec(-1) with L-serine as substrate. kcat is 0.5 sec(-1) with L-allothreonine as substrate.

substrate: L-serine


organism: Hydrogenobacter thermophilus (strain DSM 6534 / IAM 12695 / TK-6)

substrate_name: L-serine (at pH 7.5 and 70 degrees Celsius)

proof_lines: kcat is 18.7 sec(-1) for the L-serine hydroxymethyltransferase reaction, 2.3 sec(-1) for the L-threonine cleavage, and 18.0 sec(-1) for the L-allo-threonine cleavage, at 70 degrees Celsius. In the THF-independent aldolase reaction, the rate


organism: Methanosarcina barkeri (strain Fusaro / DSM 804)

substrate_name: tetrahydrofolate (at pH 7.2 and 37 degrees Celsius)

proof_lines: The apparent KM for tetrahydromethanopterin (H4MPT) is superior to 0.3 mM (at pH 7.2 and 37 degrees Celsius).


organism: Plasmodium falciparum (isolate 3D7)

proof_lines: kcat is 3.71 sec(-1) with L-serine and tetrahydrofolate as substrates (at 37 degrees Celsius) (PubMed:19591883). kcat is 9.4 min(-1) with L-serine and tetrahydrofolate as substrates (at 37 degrees Celsius) (PubMed:19591883).


enzyme_display_name: glutaminyl-peptide cyclotransferase

proof_lines: Requires an unprotonated substrate N-terminus and a protonated basic group of the enzyme for binding and conversion of the glutaminyl substrates.

enzyme: glutaminyl-peptide cyclotransferase


organism: Drosophila melanogaster

substrate_name: H-Gln-Gly-OH (at 30 degrees Celsius)

proof_lines: kcat is 9 sec(-1) with H-Gln-Gly-OH as substrate (at 30 degrees Celsius). kcat is 17 sec(-1) with H-Gln-Ala-OH as substrate (at 30 degrees Celsius). kcat is 7 sec(-1) with Gln-Gln-OH as substrate (at 30 degrees Celsius). kcat is 38 sec(-1)

substrate: H-Gln-Gly-OH (at 30 degrees Celsius)


proof_lines: kcat is 4.9 sec(-1) with H-Gln-Gly-OH as substrate (at 30 degrees Celsius). kcat is 16 sec(-1) with H-Gln-Ala-OH as substrate (at 30 degrees Celsius). kcat is 7.4 sec(-1) with Gln-Gln-OH as substrate (at 30 degrees Celsius). kcat is 14 sec(


organism: Ixodes scapularis

substrate_name: L-glutaminyl 2-naphthylamide (Gln-beta-NA) (in the presence of Zn(2+), at 25 degrees Celsius)

proof_lines: kcat is 8.8 sec(-1) for L-glutaminyl 2-naphthylamide (Gln-beta-NA) in the presence of Zn(2+). kcat is 6.9 sec(-1) for L-glutaminyl 2-naphthylamide (Gln-beta-NA) in the absence of Zn(2+).


enzyme_display_name: citrate synthase (unknown stereospecificity)

organism: Antarctic bacterium DS2-3R

substrate_name: oxaloacetate (with propionyl-CoA at pH 8 and 23 degrees Celsius)

assay_conditions_summary: pH 8; cofactor CoA

proof_lines: kcat is 8 sec(-1) for 2-methylcitrate synthase activity with propionyl-CoA as substrate (at pH 8 and 23 degrees Celsius). kcat is 21 sec(-1) for citrate synthase activity with acetyl-CoA as substrate (at pH 8 and 23 degrees Celsius).


enzyme_display_name: dye decolorizing peroxidase

organism: Thermomonospora curvata

enzyme: dye decolorizing peroxidase


substrate_name: Reactive Blue 19

substrate: Reactive Blue 19


substrate_name: Reactive Blue 4

substrate: Reactive Blue 4


substrate_name: guaiacol

substrate: guaiacol


substrate_name: hydroquinone

substrate: hydroquinone


organism: Bacillus pumilus


substrate_name: peroxyacetic acid

smiles: CC(=O)OO

substrate: peroxyacetic acid


organism: Burkholderia pseudomallei


enzyme_display_name: flavin-containing monooxygenase

organism: Homo sapiens

substrate_name: tamoxifen

enzyme: flavin-containing monooxygenase

substrate: tamoxifen


substrate_name: clomiphene

substrate: clomiphene


organism: Methylophaga aminisulfidivorans

substrate_name: indole

source_db: skid

source_license: CC BY-NC-ND 4.0

assay_conditions_summary: pH 8

substrate: indole

source: skid


organism: Rattus norvegicus

substrate_name: NADPH; L-Selenomethionine; O2; H+

assay_conditions_summary: pH 7.6; 310.15 K; cofactor NADPH

proof_lines:
pH 7.6, 37°C, purified FMO3 from liver
pH 7.6, 37°C, purified FMO3 from kidney

substrate: NADPH; L-Selenomethionine; O2; H+


assay_conditions_summary: cofactor NADPH


substrate_name: NADPH; L-Methionine; O2; H+

substrate: NADPH; L-Methionine; O2; H+


proof_lines:
Raw SKiD Env: Temp=25°C, pH=4.5
Raw SKiD Env: Temp=37°C, pH=7


organism: Escherichia coli


organism: Mycobacterium smegmatis


organism: Mycobacterium tuberculosis

substrate_name: o-Dianisidine

substrate: o-Dianisidine


organism: Synechococcus elongatus

substrate_name: isoniazid

substrate: isoniazid


organism: Agrocybe aegerita

substrate_name: H2O2; Veratryl alcohol

substrate: H2O2; Veratryl alcohol


organism: Chaetomium globosum

substrate_name: testosterone

substrate: testosterone


organism: Coprinus radians

substrate_name: H2O2; Benzyl alcohol

assay_conditions_summary: pH 7

substrate: H2O2; Benzyl alcohol


organism: Cyclocybe aegerita

substrate_name: veratryl alcohol

proof_lines: at pH 7.0, temperature not specified in the publication

substrate: veratryl alcohol


substrate_name: benzyl alcohol

substrate: benzyl alcohol


organism: Marasmius rotula

substrate_name: trans-stilbene

substrate: trans-stilbene


organism: Sulfurisphaera tokodaii

substrate_name: ethylbenzene

substrate: ethylbenzene


enzyme_display_name: procollagen-proline 4-dioxygenase

organism: Arabidopsis thaliana

substrate_name: L-Proline; O2; 2-Oxoglutarate

proof_lines:
isoform P4H1
MW: 10000-20000
isoform P4H7

enzyme: procollagen-proline 4-dioxygenase

substrate: L-Proline; O2; 2-Oxoglutarate


organism: Gallus gallus

substrate_name: O2; 2-Oxoglutarate; (Pro-Pro-Gly)10

substrate: O2; 2-Oxoglutarate; (Pro-Pro-Gly)10


substrate_name: O2; (Pro-Pro-Gly)5; 2-Oxoglutarate

substrate: O2; (Pro-Pro-Gly)5; 2-Oxoglutarate


substrate_name: NADH

proof_lines: pH and temperature not specified in the publication

substrate: NADH


substrate_name: NADPH; H+; O2; Trimethylamine

substrate: NADPH; H+; O2; Trimethylamine


substrate_name: Benzydamine

substrate: Benzydamine


substrate_name: clomiphene

substrate: clomiphene


proof_lines: mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication

proof_lines: mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 4.0, temperature not specified in the publication

proof_lines: mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication


assay_conditions_summary: pH 4

proof_lines: mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication

proof_lines: mutant enzyme G241D/R257K, at pH 4.0, temperature not specified in the publication

proof_lines: mutant enzyme G241D/R257K, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme T120V/S226G/T320N, at pH 4.4 and 30°C


proof_lines: mutant enzyme T120V/S226G/T320R, at pH 4.4 and 30°C


substrate_name: Cyclohexane

substrate: Cyclohexane


organism: Irpex lacteus


substrate_name: Reactive Blue 19

substrate: Reactive Blue 19


organism: Pleurotus sapidus


substrate_name: Reactive Blue 5

substrate: Reactive Blue 5


substrate_name: isophorone

substrate: isophorone


substrate_name: Cyclohexanol

substrate: Cyclohexanol


organism: Hypoxylon sp. EC38

proof_lines:
at pH 4.5 and 25°C
at pH 5.0 and 25°C
at pH 7.0 and 25°C


substrate_name: indole

substrate: indole


substrate_name: 3,4-dimethoxybenzyl alcohol

substrate: 3,4-dimethoxybenzyl alcohol


proof_lines:
at pH 7.0 and 24°C
at pH 5.5 and 23°C


enzyme_display_name: formate dehydrogenase

organism: [Candida] methylica

substrate_name: formate

smiles: O=CO

enzyme: formate dehydrogenase

substrate: formate


organism: Clostridium carboxidivorans

assay_conditions_summary: pH 6.8; 310.15 K; cofactor NADH


organism: Cupriavidus necator


organism: Lotus japonicus

assay_conditions_summary: pH 7.5; 303.15 K; buffer note rather than structured fields; cofactor NADP+

proof_lines: in 0.1 M potassium phosphate buffer, pH 7.5, at 30°C


organism: Arthrobacter sp.

substrate_name: oxaloacetate

smiles: O=C(O)CC(=O)C(=O)O

assay_conditions_summary: pH 8; 296.15 K; cofactor CoA

enzyme: citrate synthase (unknown stereospecificity)

substrate: oxaloacetate


substrate_name: Acetyl-CoA

substrate: Acetyl-CoA


organism: Aspergillus nidulans

substrate_name: acetyl-CoA

proof_lines: with propionyl-CoA, methylcitrate synthase exhibiting also citrate synthase activity

substrate: acetyl-CoA


organism: Aspergillus niger

assay_conditions_summary: pH 7.5; cofactor CoA


organism: Bacillus subtilis

substrate_name: H2O; Acetyl-CoA; Oxaloacetate

substrate: H2O; Acetyl-CoA; Oxaloacetate


organism: Escherichia coli

proof_lines: mutant enzyme G181E, pH and temperature not specified in the publication


proof_lines: wild type enzyme, pH and temperature not specified in the publication


organism: Xanthomonas campestris


organism: Acinetobacter calcoaceticus (subsp. anitratus)

assay_conditions_summary: cofactor NADH


assay_conditions_summary: pH 7.8; 303.15 K; cofactor CoA


proof_lines:
at pH 8.0 and 20°C
Raw SKiD Env: Temp=20°C, pH=8


organism: Methanobacterium formicicum

substrate_name: Coenzyme F420; Formate

substrate: Coenzyme F420; Formate


organism: Moraxella sp.

assay_conditions_summary: pH 5.5; 298.15 K; buffer condition; cofactor NAD+

proof_lines: in the presence of 0.005 mM Na+, at pH 5.5 and 25°C

proof_lines: in the absence of metal ion, at pH 5.5 and 25°C

proof_lines: in 0.1 M potassium phosphate buffer pH 7.0, at 30°C


organism: Ogataea angusta

assay_conditions_summary: pH 6.5; 303.15 K; cofactor NAD+


organism: Pseudomonas sp. 101

substrate_name: NAD+

assay_conditions_summary: cofactor NADP+

substrate: NAD+


organism: Thermochaetoides thermophila

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADH


assay_conditions_summary: pH 7.4; 298.15 K; cofactor NAD+


organism: Thermothelomyces thermophilus

substrate_name: NADH

assay_conditions_summary: pH 6; 308.15 K; cofactor NADH

substrate: NADH


smiles: O=C=O


assay_conditions_summary: pH 10.5; 308.15 K; cofactor NAD+


assay_conditions_summary: pH 10.5; 308.15 K; cofactor NADH


organism: Homo sapiens

substrate_name: Gln-Gln

proof_lines: pH and temperature not specified in the publication, secretory glutaminyl cyclase

substrate: Gln-Gln


organism: Sus scrofa

substrate_name: NADPH; H+; O2; Trimethylamine

assay_conditions_summary: cofactor NADPH

substrate: NADPH; H+; O2; Trimethylamine


organism: Arabidopsis thaliana

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NAD+


organism: Bacillus sp. (in: Bacteria)

assay_conditions_summary: pH 7; 303.15 K; cofactor NAD+


substrate_name: NADP+

assay_conditions_summary: pH 7; 303.15 K; cofactor NADP+

substrate: NADP+


organism: Candida boidinii

substrate_name: NAD+; Formate

assay_conditions_summary: cofactor NAD+

substrate: NAD+; Formate


organism: [Candida] boidinii


assay_conditions_summary: pH 7.5; 298.15 K; cofactor NAD+


assay_conditions_summary: pH 9.5; 303.15 K; cofactor NAD+


assay_conditions_summary: pH 9.5; 303.15 K; cofactor NADH


assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADH


organism: [Candida] methylica

assay_conditions_summary: pH 8; 298.15 K; cofactor NADH


organism: Microcystis aeruginosa

assay_conditions_summary: pH 8; 298.15 K; cofactor CoA


organism: Nostoc sp. PCC 7120 = FACHB-418


organism: Saccharolobus solfataricus

assay_conditions_summary: 298.15 K; cofactor CoA

proof_lines: 25°C, pH and temperature not specified in the publication, competitive inhibition versus acetyl-CoA

proof_lines: 25°C, pH and temperature not specified in the publication, non-competitive inhibition versus oxaloacetate


organism: Saccharomyces cerevisiae

assay_conditions_summary: pH 7.5; 310.15 K; cofactor CoA


organism: Streptomyces diastaticus

assay_conditions_summary: pH 8; 308.15 K; cofactor CoA


organism: Synechocystis sp. PCC 6803

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NADH

proof_lines: presence of 5 mM phosphoenolpyruvate, pH 7.5, 37°C


organism: Escherichia coli

assay_conditions_summary: cofactor CoA

proof_lines: mutant enzyme T204R, pH and temperature not specified in the publication


assay_conditions_summary: pH 8; 310.15 K; cofactor CoA


assay_conditions_summary: pH 7.8; 298.15 K; cofactor CoA

proof_lines:
Raw SKiD Env: Temp=-----, pH=and
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=25°C, pH=7.8


organism: Fasciola hepatica


organism: Geobacter sulfurreducens

assay_conditions_summary: pH 8; 303.15 K; cofactor CoA

proof_lines:
30°C, pH 8, recombinant enzyme
30°C, pH 8, native enzyme
30°C, pH 8, versus acetyl-CoA


organism: Halobacterium volcanii


organism: Haloferax volcanii

proof_lines:
30°C, pH 8.0, native enzyme
30°C, pH 8.0, recombinant enzyme


organism: Helicobacter pylori


organism: Metallosphaera sedula

proof_lines: pH not specified in the publication, temperature not specified in the publication


enzyme_display_name: thymidine kinase

organism: Homo sapiens

substrate_name: ATP; Thymidine

enzyme: thymidine kinase

substrate: ATP; Thymidine


proof_lines:
tetrameric enzyme, pH 7.5, 25°C
dimeric enzyme, pH 7.5, 25°C


assay_conditions_summary: pH 7.5; cofactor ATP

proof_lines: mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication


proof_lines: mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication


substrate_name: thymidine

proof_lines: mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication

substrate: thymidine


proof_lines: wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication


proof_lines: mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication


organism: Human alphaherpesvirus 1

proof_lines: mutant enzyme P12N/H13I, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C


proof_lines: wild type enzyme, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C


proof_lines: mutant enzyme P12N, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C


assay_conditions_summary: pH 8; 310.15 K; buffer Tris/HCl

proof_lines: mutant enzyme P12N/H13I/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C


proof_lines: mutant enzyme H13I/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C


proof_lines: mutant enzyme H13I, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C


proof_lines: mutant enzyme P12N/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C


organism: Human herpesvirus 1

substrate_name: Ganciclovir

substrate: Ganciclovir


assay_conditions_summary: cofactor ATP


substrate_name: acyclovir

substrate: acyclovir


organism: Staphylococcus aureus


organism: Thermotoga maritima

assay_conditions_summary: 355.15 K; cofactor ATP

proof_lines: 82°C, pH not specified in the publication

proof_lines: 37°C, pH not specified in the publication


substrate_name: ATP

assay_conditions_summary: pH 7.2; 310.15 K; cofactor ATP

substrate: ATP


substrate_name: ATP; Deoxythymidine

substrate: ATP; Deoxythymidine


organism: Epstein-Barr virus

substrate_name: CTP

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

substrate: CTP


substrate_name: dTTP

substrate: dTTP


organism: Gallus gallus

assay_conditions_summary: pH 8; cofactor ATP

proof_lines: in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication

proof_lines: in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication


assay_conditions_summary: pH 7.6; cofactor ATP

proof_lines: wild-type, thymidine as substrate, pH 7.6


proof_lines: I212 mutant, thymidine as substrate, pH 7.6

proof_lines: I212 mutant, deoxycytidine as substrate, pH 7.6


proof_lines: H121N mutant, thymidine as substrate, pH 7.6

proof_lines: H121N mutant, deoxycytidine as substrate, pH 7.6


substrate_name: oxaloacetate

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ADP

substrate: oxaloacetate


substrate_name: acetyl-CoA

substrate: acetyl-CoA


organism: Thermoplasma acidophilum


proof_lines: D317N mutant protein, value is very uncertain


organism: Thermosulfidibacter takaii

substrate_name: H2O; Acetyl-CoA; Oxaloacetate

substrate: H2O; Acetyl-CoA; Oxaloacetate


organism: Anolis carolinensis


organism: Arabidopsis thaliana

substrate_name: deoxyuridine

proof_lines: isoform TK1a, pH and temperature not specified in the publication

proof_lines: isoform TK1b, pH and temperature not specified in the publication

substrate: deoxyuridine


proof_lines: subunit alpha, in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication

proof_lines: subunit alpha, in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication

proof_lines: subunit beta, in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication


organism: Bacillus cereus


organism: Caenorhabditis elegans


organism: Danio rerio


organism: Dictyostelium discoideum


assay_conditions_summary: pH 8; buffer ATP present versus ATP absent in Tris; cofactor ATP


organism: Entamoeba histolytica

assay_conditions_summary: pH 8; 310.15 K; buffer an assay-condition note (potassium phosphate; cofactor ATP

proof_lines: at 37°C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2


substrate_name: GTP

substrate: GTP


organism: Vaccinia virus


substrate_name: Deoxythymidine; ATP

substrate: Deoxythymidine; ATP


organism: Xenopus tropicalis


enzyme_display_name: aryl sulfotransferase

organism: Bos taurus

substrate_name: Unknown

assay_conditions_summary: pH 7.4; 298.15 K; cofactor CoA

proof_lines: pH 7.4, 25°C, recombinant SULT1A1, method B

proof_lines: pH 7.4, 25°C, recombinant SULT1A1, method A

enzyme: aryl sulfotransferase

substrate: Unknown


organism: Homo sapiens


substrate_name: 3'-Phosphoadenylyl sulfate; Dopamine

substrate: 3'-Phosphoadenylyl sulfate; Dopamine


organism: Leishmania major


substrate_name: uridine

substrate: uridine


assay_conditions_summary: pH 7.4; 298.15 K; cofactor ATP

proof_lines: K0.5 value, Hill coefficient 1.6, pH 7.4, 25°C


organism: Mus musculus

substrate_name: deoxycytidine

substrate: deoxycytidine


substrate_name: ATP; 1-beta-D-Arabinofuranosylthymine

substrate: ATP; 1-beta-D-Arabinofuranosylthymine


substrate_name: ATP; Thymidine

substrate: ATP; Thymidine


organism: Rattus norvegicus

proof_lines: in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication


organism: Saccharolobus solfataricus

substrate_name: delta-undecanolactone

substrate: delta-undecanolactone


substrate_name: paraoxon

substrate: paraoxon


substrate_name: gamma-undecanolactone

substrate: gamma-undecanolactone


substrate_name: N-(3-oxodecanoyl)-L-homoserine lactone

substrate: N-(3-oxodecanoyl)-L-homoserine lactone


substrate_name: gamma-heptanolactone

substrate: gamma-heptanolactone


substrate_name: gamma-nonalactone

substrate: gamma-nonalactone


substrate_name: N-(3-oxohexanoyl)-L-homoserine lactone

substrate: N-(3-oxohexanoyl)-L-homoserine lactone


substrate_name: gamma-dodecanolactone

substrate: gamma-dodecanolactone


substrate_name: N-(3-oxooctanoyl)-L-homoserine lactone

substrate: N-(3-oxooctanoyl)-L-homoserine lactone


substrate_name: epsilon-caprolactone

substrate: epsilon-caprolactone


substrate_name: N-hexanoylhomoserine lactone

substrate: N-hexanoylhomoserine lactone


substrate_name: delta-nonalactone

substrate: delta-nonalactone


enzyme_display_name: beta-fructofuranosidase

substrate_name: Sucrose

enzyme: beta-fructofuranosidase

substrate: Sucrose


substrate_name: H2O; Sucrose

substrate: H2O; Sucrose


substrate_name: delta-valerolactone

substrate: delta-valerolactone


substrate_name: N-octanoylhomoserine lactone

substrate: N-octanoylhomoserine lactone


substrate_name: dihydrocoumarin

substrate: dihydrocoumarin


substrate_name: N-(3-Oxooctanoyl)homoserine lactone

substrate: N-(3-Oxooctanoyl)homoserine lactone


substrate_name: N-dodecanoylhomoserine lactone

substrate: N-dodecanoylhomoserine lactone


substrate_name: delta-undecanolactone

substrate: delta-undecanolactone


substrate_name: N-(3-oxohexanoyl)homoserine lactone

substrate: N-(3-oxohexanoyl)homoserine lactone


organism: Sulfolobus acidocaldarius


substrate_name: gamma-heptanolide

substrate: gamma-heptanolide


organism: Lactococcus lactis subsp. lactis

substrate_name: Sucrose 6-phosphate; H2O

substrate: Sucrose 6-phosphate; H2O


substrate_name: sucrose

substrate: sucrose


substrate_name: inulin

substrate: inulin


substrate_name: raffinose

substrate: raffinose


substrate_name: stachyose

substrate: stachyose


organism: Nostoc sp. PCC 7120 = FACHB-418

proof_lines: pH and temperature not specified in the publication


proof_lines: pH 7.7, 30°C, invertase InvB, wild-type enzyme


proof_lines: pH 7.9, 30°C, invertase InvA, mutant enzyme R429N


proof_lines: pH 7.9, 30°C, invertase InvA, wild-type enzyme


assay_conditions_summary: pH 7; 310.15 K; buffer 50 mM Tris/HCl

proof_lines:
SULT1A1 allozyme 2, pH 7.0, 37°C
pH 6.5, 37°C, allozyme SULT1A1*2
pH 6.5, 37°C, allozyme SULT1A1*3


substrate_name: 3'-Phosphoadenylyl sulfate; Dopamine

substrate: 3'-Phosphoadenylyl sulfate; Dopamine


proof_lines:
SULT1B1, pH 7.4, 37°C
Raw SKiD Env: Temp=37°C, pH=7
Raw SKiD Env: Temp=37°C, pH=7.4


substrate_name: serotonin

proof_lines: dopamine 3-O-sulfate formation, recombinant SULT1A3

substrate: serotonin


substrate_name: 3'-Phosphoadenylyl sulfate; 2-Naphthol

substrate: 3'-Phosphoadenylyl sulfate; 2-Naphthol


organism: Clonostachys rosea

substrate_name: zearalenone

substrate: zearalenone


substrate_name: delta-valerolactone

assay_conditions_summary: pH 8; buffer pH 8

proof_lines: pH 8.3, activity buffer with 0.1% tergitol

proof_lines: isoform PON1, pH and temperature not specified in the publication

substrate: delta-valerolactone


substrate_name: Gamma-caprolactone

assay_conditions_summary: pH 8

substrate: Gamma-caprolactone


substrate_name: H2O; delta-Valerolactone

substrate: H2O; delta-Valerolactone


organism: Arabidopsis thaliana

substrate_name: Sucrose

substrate: Sucrose


organism: Aspergillus niger

assay_conditions_summary: pH 5; 310.15 K; buffer 50 mM acetate

proof_lines: in 50 mM acetate buffer (pH 5.0) at 37°C


organism: Aureobasidium melanogenum


organism: Bombyx mori


organism: Daucus carota

substrate_name: H2O; Sucrose

substrate: H2O; Sucrose


organism: Elsholtzia haichowensis


organism: Fusobacterium mortiferum


organism: Glycine max


organism: Ipomoea batatas


assay_conditions_summary: pH 5

proof_lines: pH 5.0, recombinant isozyme lbbetafruct3


proof_lines: pH 5.0, recombinant isozyme lbbetafruct2


organism: Klebsiella pneumoniae


organism: Lactobacillus reuteri


organism: Sulfolobus islandicus


substrate_name: gamma-butyrolactone

substrate: gamma-butyrolactone


substrate_name: dodecanoic-gamma-lactone

substrate: dodecanoic-gamma-lactone


substrate_name: epsilon-caprolactone

substrate: epsilon-caprolactone


substrate_name: dodecanoic-delta-lactone

substrate: dodecanoic-delta-lactone


substrate_name: undecanoic-gamma-lactone

substrate: undecanoic-gamma-lactone


organism: Vulcanisaeta moutnovskia

substrate_name: gamma-nonalactone

substrate: gamma-nonalactone


substrate_name: delta-undecalactone

substrate: delta-undecalactone


substrate_name: delta-dodecalactone

substrate: delta-dodecalactone


substrate_name: gamma-dodecalactone

substrate: gamma-dodecalactone


substrate_name: 3-oxo-decanoyl-L-homoserine lactone

substrate: 3-oxo-decanoyl-L-homoserine lactone


substrate_name: delta-nonalactone

substrate: delta-nonalactone


substrate_name: gamma-undecalactone

substrate: gamma-undecalactone


substrate_name: octanoyl-L-homoserine lactone

substrate: octanoyl-L-homoserine lactone


substrate_name: gamma-heptalactone

substrate: gamma-heptalactone


substrate_name: 3-oxo-octanoyl-L-homoserine lactone

substrate: 3-oxo-octanoyl-L-homoserine lactone


enzyme_display_name: dGTPase

organism: Escherichia coli

substrate_name: H2O; dGTP

enzyme: dGTPase

substrate: H2O; dGTP


organism: Allium cepa

substrate_name: sucrose

substrate: sucrose


organism: Dengue virus type 2


organism: Zika virus


enzyme_display_name: cathepsin L

organism: Rattus norvegicus

enzyme: cathepsin L


proof_lines: pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma

proof_lines: enzyme immobilized on amino functionalized magnetic Ni(0.3)Mg(0.4)Zn(0.3)Fe2O4 nanoparticles, pH and temperature not specified in the publication


substrate_name: Benzylpenicillin; H2O

substrate: Benzylpenicillin; H2O


organism: Kluyvera citrophila

substrate_name: H2O; Penicillin G

substrate: H2O; Penicillin G


organism: Bacillus subtilis


organism: Bacteroides ovatus


substrate_name: H2O; Xyloglucan

substrate: H2O; Xyloglucan


substrate_name: p-Nitrophenyl-beta-D-xylopyranoside

substrate: p-Nitrophenyl-beta-D-xylopyranoside


organism: Bifidobacterium longum


organism: Geobacillus stearothermophilus


organism: Hordeum vulgare


organism: Medicago sativa


organism: Lysinibacillus sphaericus

substrate_name: Penicillin V

substrate: Penicillin V


organism: Thermus thermophilus

substrate_name: penicillin K

substrate: penicillin K


substrate_name: penicillin G

substrate: penicillin G


enzyme_display_name: tryptophan synthase

organism: Archaeoglobus fulgidus

substrate_name: indole

enzyme: tryptophan synthase

substrate: indole


substrate_name: (3-Indolyl)-glycerol phosphate

substrate: (3-Indolyl)-glycerol phosphate


substrate_name: Indole

substrate: Indole


organism: Paenibacillus sp.

substrate_name: 4-nitrophenyl alpha-L-arabinofuranoside

substrate: 4-nitrophenyl alpha-L-arabinofuranoside


substrate_name: 4-nitrophenyl beta-D-xylopyranoside

substrate: 4-nitrophenyl beta-D-xylopyranoside


substrate_name: 4-nitrophenyl beta-D-galactofuranoside

substrate: 4-nitrophenyl beta-D-galactofuranoside


organism: Podospora anserina


organism: Pseudothermotoga thermarum


organism: Streptomyces coelicolor


organism: Talaromyces purpureogenus


organism: Thermobacillus xylanilyticus

substrate_name: p-nitrophenyl-alpha-L-arabinofuranose

proof_lines:
Tx-Abf, 60°C
Raw SKiD Env: Temp=45°C, pH=7
Raw SKiD Env: Temp=60°C, pH=5.8

substrate: p-nitrophenyl-alpha-L-arabinofuranose


organism: uncultured rumen bacterium


organism: Ustilago maydis


organism: Weissella sp. 142


enzyme_display_name: flavivirin

organism: Dengue virus type 2

enzyme: flavivirin


organism: Nostoc sp. PCC 7120 = FACHB-418

proof_lines: pH 7.9, 30°C, invertase InvA, mutant enzyme R429A


proof_lines: pH 7.7, 30°C, invertase InvB, mutant enzyme R430N


organism: Phaffia rhodozyma

proof_lines: pH 5.5, 42°C, alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranosyl-(1,2)-beta-D-fructofuranose


organism: Phaseolus aureus

substrate_name: H2O; Sucrose

substrate: H2O; Sucrose


organism: Pyrus pyrifolia


organism: Schwanniomyces occidentalis

proof_lines: pH and temperature not specified in the publication


organism: Solanum lycopersicum


organism: Streptococcus mutans

substrate_name: Sucrose 6-phosphate; H2O

substrate: Sucrose 6-phosphate; H2O


organism: uncultured bacterium

substrate_name: sucrose

substrate: sucrose


organism: Uromyces viciae-fabae

assay_conditions_summary: pH 4.6; 308.15 K; buffer an unstructured assay-condition note (sodium acetate

proof_lines: in 100 mM Na-acetate, pH 4.6, and 100 mM sucrose, at 35°C


organism: Wickerhamomyces anomalus


organism: Alicyclobacillus sp.


organism: Arabidopsis thaliana


organism: Aspergillus kawachi


organism: Bacillus pumilus


organism: Bacillus subtilis

substrate_name: arabinoxylan

substrate: arabinoxylan


substrate_name: Arabinotriose

substrate: Arabinotriose


substrate_name: Arabinobiose

substrate: Arabinobiose


organism: Escherichia coli


organism: Thermotoga maritima

substrate_name: L-Serine

substrate: L-Serine


substrate_name: L-Serine; Indole

substrate: L-Serine; Indole


proof_lines: alpha-subunit, mutant T183V/A180G, pH 7.6, 25°C


substrate_name: L-serine

substrate: L-serine


proof_lines: cosubstrate 5-hydroxyindole, mutant A67M, pH 8.5, 35Â°C


proof_lines: cosubstrate 5-hydroxyindole, mutant P257R, pH 8.5, 35Â°C


organism: Pyrococcus furiosus


proof_lines: cosubstrate L-serine, pH 8.0, temperature not specified in the publication

proof_lines: cosubstrate L-threonine, pH 8.0, temperature not specified in the publication


organism: Saccharolobus solfataricus


substrate_name: O-phospho-L-serine

substrate: O-phospho-L-serine


organism: Thermococcus kodakarensis

substrate_name: indole

substrate: indole


substrate_name: (3-Indolyl)-glycerol phosphate

substrate: (3-Indolyl)-glycerol phosphate


substrate_name: Indole

substrate: Indole


enzyme_display_name: dihydrolipoyl dehydrogenase

organism: Homo sapiens

substrate_name: Lipoamide

enzyme: dihydrolipoyl dehydrogenase

substrate: Lipoamide


substrate_name: NAD+

assay_conditions_summary: cofactor NAD+

substrate: NAD+


substrate_name: dihydrolipoamide

assay_conditions_summary: pH 8; 310.15 K; cofactor NAD+

proof_lines: wild-type, pH 8.0, 37°C, presence of 1.5 mM EDTA

substrate: dihydrolipoamide