organism: Ovis aries

substrate_name: L-Ser

substrate: L-Ser


enzyme_display_name: glycine hydroxymethyltransferase

source_db: brenda

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

protein_accession_database: UniProt

source_license: CC BY 4.0

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

enzyme: glycine hydroxymethyltransferase

source: brenda


organism: Malus domestica

substrate_name: phosphoenolpyruvate

kcat_display: Not reported

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution

proof_lines: mutant enzyme A187T, at 28°C, pH not specified in the publication

substrate: phosphoenolpyruvate


proof_lines: wild type enzyme, at 28°C, pH not specified in the publication


proof_lines: mutant enzyme T101A/A187T, at 28°C, pH not specified in the publication


proof_lines: mutant enzyme T101A, at 28°C, pH not specified in the publication


organism: Idiomarina loihiensis

substrate_name: DL-phenylserine

substrate: DL-phenylserine


proof_lines: mutant enzyme H61G/G132P, at pH 7.0 and 37°C


organism: Bombyx mori

substrate_name: tetrahydrofolate

assay_conditions_summary: pH 3; 303.15 K; cofactor NADP+

proof_lines: wild type enzyme, in the presence of NADP+, at pH 3.0 and 30°C

substrate: tetrahydrofolate


proof_lines: mutant enzyme H119A, in the presence of NADP+, at pH 3.0 and 30°C


proof_lines: mutant enzyme H132A, in the presence of NADP+, at pH 3.0 and 30°C


proof_lines: mutant enzyme H135A, in the presence of NADP+, at pH 3.0 and 30°C


enzyme_display_name: sulfite oxidase

organism: Homo sapiens

substrate_name: sulfite

enzyme: sulfite oxidase

substrate: sulfite


proof_lines:
mutant R472D, pH 6.5, 25°C
mutant R472D, pH 7.6, 25°C


enzyme_display_name: unspecific peroxygenase

organism: Achaetomiella virescens

substrate_name: Naphthalene

assay_conditions_summary: 298.15 K; buffer acetate

proof_lines: mutant enzyme F88L, at 25°C, pH not specified in the publication

enzyme: unspecific peroxygenase

substrate: Naphthalene


proof_lines: wild type enzyme, at 25°C, pH not specified in the publication


proof_lines: mutant enzyme T158F, at 25°C, pH not specified in the publication


substrate_name: benzyl alcohol

km_display: Not reported

proof_lines: at 25°C, pH not specified in the publication

substrate: benzyl alcohol


enzyme_display_name: citrate synthase (unknown stereospecificity)

organism: Citrullus lanatus

substrate_name: Unknown

ki_unit: mM

proof_lines:
glyoxysomal isozyme
mitochondrial isozyme

substrate: Unknown


organism: Pseudomonas aeruginosa

substrate_name: acetyl-CoA

assay_conditions_summary: cofactor CoA

proof_lines:
CS I
isoenzyme CSI
CS II

enzyme: citrate synthase (unknown stereospecificity)

substrate: acetyl-CoA


organism: Saccharomyces cerevisiae

proof_lines: native and recombinant peroxisomal isozyme CS II


enzyme_display_name: aryl sulfotransferase

substrate_name: 3'-Phosphoadenylyl sulfate; Dopamine

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

proof_lines: enzyme form SULT1A3, 4-sulfate formation

proof_lines: enzyme form SULT1A1, 7-sulfate formation

proof_lines: enzyme form SULT1E1, 7-sulfate formation

enzyme: aryl sulfotransferase

substrate: 3'-Phosphoadenylyl sulfate; Dopamine


enzyme_display_name: beta-fructofuranosidase

organism: Phyllostachys edulis

substrate_name: sucrose

proof_lines:
pH 7.0, 37°C, isozyme IT I
pH 5.0, 37°C, isozyme IT II

enzyme: beta-fructofuranosidase

substrate: sucrose


organism: Pyrus pyrifolia

proof_lines:
pH 4.5, 30°C, isoenzyme AIV I
pH 4.5, 30°C, isoenzyme AIV II


organism: Unknown


enzyme_display_name: alkaline phosphatase

organism: Vibrio sp.

substrate_name: 4-nitrophenyl phosphate

enzyme: alkaline phosphatase

substrate: 4-nitrophenyl phosphate


enzyme_display_name: thymidine kinase

substrate_name: thymidine

proof_lines: thymidine kinase-2 mutant enzyme I59Y, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C

enzyme: thymidine kinase

substrate: thymidine


proof_lines: thymidine kinase-2 mutant enzyme I59H, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C


proof_lines: thymidine kinase-2 mutant enzyme I59L, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C


proof_lines: thymidine kinase-2 mutant enzyme I59V, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C


organism: Human gammaherpesvirus 4

substrate_name: ATP

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

proof_lines: about, recombinant mutant K297R, pH 7.5, 37°C

substrate: ATP


substrate_name: GTP

substrate: GTP


proof_lines: recombinant wild-type enzyme, pH 7.5, 37°C


substrate_name: TTP

proof_lines: about, recombinant mutant T298S, pH 7.5, 37°C

substrate: TTP


substrate_name: 5-enolpyruvylshikimate 3-phosphate

substrate: 5-enolpyruvylshikimate 3-phosphate


substrate_name: phosphate

proof_lines:
25°C, pH 7.0, mutant enzyme G99A
+/-0.03, mutant G99A
+/-0.04, mutant G99A

substrate: phosphate


proof_lines:
25°C, pH 7.0, mutant strain L104P
+/-0.1, mutant L104P
25°C, pH 7.0, mutant enzyme L104P


enzyme_display_name: arginase

organism: Rattus norvegicus

substrate_name: L-arginine

assay_conditions_summary: pH 9

enzyme: arginase

substrate: L-arginine


substrate_name: p-nitrophenyl phosphate

substrate: p-nitrophenyl phosphate


proof_lines:
25°C, pH 9.8, wild-type enzyme
25°C, pH 8.0, wild-type enzyme
25°C, pH 8.0, wild-.type enzyme


organism: Streptomyces olivaceoviridis

substrate_name: p-nitrophenyl-beta-D-xylobioside

substrate: p-nitrophenyl-beta-D-xylobioside


enzyme_display_name: phosphoenolpyruvate carboxykinase (GTP)

organism: Anaerobiospirillum succiniciproducens

substrate_name: oxaloacetate

enzyme: phosphoenolpyruvate carboxykinase (GTP)

substrate: oxaloacetate


proof_lines: pH 7.5, 30°C, above, D263N and H225Q mutants


enzyme_display_name: phenylalanine ammonia-lyase

organism: Petroselinum crispum

substrate_name: L-phenylalanine

enzyme: phenylalanine ammonia-lyase

substrate: L-phenylalanine


proof_lines:
pH 8.8, 30°C, mutant enzyme F137V
mutant F137V, pH 10, 30°C


substrate_name: L-styrylalanine

substrate: L-styrylalanine


proof_lines:
pH 8.8, 30°C, wild-type enzyme
wild-type, pH 10, 30°C


substrate_name: L-Phe

substrate: L-Phe


enzyme_display_name: glutamine synthetase

organism: Bacillus subtilis

assay_conditions_summary: 298.15 K; cofactor ATP

proof_lines: mutant Y303H, 25°C, pH not specified in the publication

enzyme: glutamine synthetase


proof_lines: mutant G302A, 25°C, pH not specified in the publication


proof_lines: mutant E304H, 25°C, pH not specified in the publication


substrate_name: hydroxylamine

proof_lines: mutant E304D, 25°C, pH not specified in the publication

substrate: hydroxylamine


enzyme_display_name: dihydrolipoyl dehydrogenase

organism: Escherichia coli K-12

substrate_name: NAD+

assay_conditions_summary: cofactor NAD+

proof_lines:
native LPD
native pyruvate dehydrogenase complex

enzyme: dihydrolipoyl dehydrogenase

substrate: NAD+


substrate_name: pyruvate

substrate: pyruvate


enzyme_display_name: phenylalanine dehydrogenase

organism: Thermoactinomyces intermedius

enzyme: phenylalanine dehydrogenase


substrate_name: phenylpyruvate

substrate: phenylpyruvate


organism: Homo sapiens

substrate_name: sulfite

assay_conditions_summary: pH 7

proof_lines:
mutant enzyme G473D, pH 7.0
mutant enzyme G473D, pH 6.5
mutant enzyme G473D, pH 6.0

substrate: sulfite


assay_conditions_summary: pH 6

proof_lines: mutant enzyme G473A in 20 mM Tris at pH 6.0


substrate_name: cytochrome c

proof_lines: mutant enzyme G473W in 20 mM Tris at pH 6.0

substrate: cytochrome c


organism: Ovis aries

substrate_name: L-serine

substrate: L-serine


substrate_name: tetrahydrofolate

proof_lines: mutant S394N, in the presence of pyridoxal phosphate

substrate: tetrahydrofolate


proof_lines: mutant L474F, in the presence of pyridoxal phosphate


proof_lines: wild-type, in the presence of pyridoxal phosphate


enzyme_display_name: aspartate transaminase

organism: Gallus gallus

proof_lines:
recombinant C166S mutant, pH 7.5, 25°C
recombinant mutant C166S, pH 7.5, 25°C

enzyme: aspartate transaminase


proof_lines:
recombinant C166A mutant, pH 7.5, 25°C
recombinant mutant C166A, pH 7.5, 25°C


mutation_signature: recombinant deletion mutant

mutation_type: deletion

proof_lines: recombinant deletion mutant, pH 7.5, 25°C


substrate_name: ATP

assay_conditions_summary: cofactor ATP

substrate: ATP


assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

proof_lines: pH 7.5, 30°C, liver, comparison of Km of wild-type, mutant and mutant phosphorylated enzyme


organism: Alcanivorax sp.

substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


organism: Eleusine indica

proof_lines:
glyphosate-resistant strain
glyphosate-sensitive strain


substrate_name: Unknown

substrate: Unknown


enzyme_display_name: alpha-galactosidase

enzyme: alpha-galactosidase


organism: Herpes simplex virus

substrate_name: thymidine

substrate: thymidine


substrate_name: ganciclovir

substrate: ganciclovir


organism: Helicobacter pylori

substrate_name: L-arginine

proof_lines:
wild-type, pH 7.2, 37°C
wild-type, Co2+-activated, pH 5.0, 37°C
wild-type, Mn2+-activated, pH 5.0, 37°C

substrate: L-arginine


substrate_name: agmatine

substrate: agmatine


organism: Trichormus variabilis

substrate_name: L-phenylalanine

substrate: L-phenylalanine


enzyme_display_name: catalase peroxidase

organism: Synechocystis sp.

assay_conditions_summary: pH 7; 303.15 K; buffer 50 mM phosphate

proof_lines: catalase activity, mutant enzyme N153A, in 50 mM phosphate buffer, pH 7.0, and 30°C

enzyme: catalase peroxidase


proof_lines: catalase activity, mutant enzyme N153D, in 50 mM phosphate buffer, pH 7.0, and 30°C


proof_lines: mutant lacking 21 N-terminal amino acids, pH 8.5, 37°C


organism: Candolleomyces aberdarensis


substrate_name: veratryl alcohol

proof_lines: mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C

substrate: veratryl alcohol


organism: Daldinia caldariorum

proof_lines: wild type enzyme, at 25°C, pH not specified in the publication


substrate_name: Naphthalene

proof_lines: at 25°C, pH not specified in the publication

substrate: Naphthalene


enzyme_display_name: ferredoxin--NADP(+) reductase

organism: Anabaena sp.

substrate_name: reduced flavodoxin

assay_conditions_summary: pH 8; 298.15 K; cofactor NADPH

proof_lines: recombinant mutant E139K, pH 8.0, 25°C, ionic strength of 200 mM

proof_lines: recombinant mutant E139K, pH 8.0, 25°C, ionic strength of 28 mM

proof_lines: recombinant mutant E139K, diaphorase activity with 2,6-dichlorophenolindophenol, pH 8.0, 25°C, ionic strength of 28 mM

enzyme: ferredoxin--NADP(+) reductase

substrate: reduced flavodoxin


substrate_name: reduced ferredoxin

proof_lines: recombinant mutant E139Q, pH 8.0, 25°C, ionic strength of 28 mM

proof_lines: recombinant mutant E139Q, pH 8.0, 25°C, ionic strength of 200 mM

proof_lines: recombinant mutant E139Q, pH 8.0, 25°C, ionic strength of 100 mM

substrate: reduced ferredoxin


substrate_name: L-ethionine

substrate: L-ethionine


enzyme_display_name: flavin-containing monooxygenase

organism: Methylophaga sp.

substrate_name: trimethylamine

assay_conditions_summary: pH 8.5; 298.15 K; cofactor NADPH

proof_lines: pH 8.0, 25°C, recombinant wild-type enzyme

enzyme: flavin-containing monooxygenase

substrate: trimethylamine


substrate_name: dihydrolipoamide

assay_conditions_summary: pH 8; 310.15 K; cofactor NAD+

proof_lines: mutant W366A, pH 8.0, 37°C, presence of 1.5 mM EDTA

substrate: dihydrolipoamide


proof_lines: mutant P325A, pH 8.0, 37°C, presence of 1.5 mM EDTA


enzyme_display_name: glycine hydroxymethyltransferase

organism: Trichomonas vaginalis

proof_lines: mutant K251R, without pyridoxal phosphate

proof_lines: mutant K251R, in the presence of 0.25 mM pyridoxal phosphate

enzyme: glycine hydroxymethyltransferase


proof_lines: wild-type, in the presence of 0.25 mM pyridoxal phosphate


organism: Escherichia coli

substrate_name: shikimate 3-phosphate

substrate: shikimate 3-phosphate


substrate_name: phosphate

substrate: phosphate


organism: Stutzerimonas stutzeri

proof_lines: +/-0.01 mM, in presence of 1 mM shikimate 3-phosphate and 0-500 mM inhibitor, bacterial extract


proof_lines: +/-0.0014 mM, mutant N130S in bacterial extact

proof_lines: +/-0.01 mM, mutant N130S, in presence of 1 mM shikimate 3-phosphate and 0-500 mM inhibitor, bacterial extract


proof_lines:
wild type enzyme, at pH 8.0 and 40°C
wild-type enzyme
wild-type, pH 8.4, 25°C


substrate_name: 2-oxo-4-phenyl-butanoic acid

substrate: 2-oxo-4-phenyl-butanoic acid


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


substrate_name: beta-D-fructose 6-phosphate

proof_lines: pH 7.5, 30°C, comparison of Km of wild-type, mutant and mutant phosphorylated enzyme

substrate: beta-D-fructose 6-phosphate


substrate_name: ATP

proof_lines:
pH 7.5, 30°C
pH 7.1, 30°C, native chicken enzyme
pH 7.5, 30°C, kinetic study

substrate: ATP


organism: Badhamia polycephala

substrate_name: thymidine

proof_lines: TK-A, above 0.004 mM deoxythymidine, 25°C

proof_lines: TK-C, below 0.004 mM deoxythymidine, 25°C

proof_lines: TK-C, above 0.004 mM deoxythymidine, 25°C

substrate: thymidine


substrate_name: Unknown

proof_lines:
variant TK-A, 25°C
variant TK-C, 25°C

substrate: Unknown


enzyme_display_name: arginine kinase

organism: Sabellastarte spectabilis

substrate_name: L-Arg

assay_conditions_summary: pH 8; 298.15 K; cofactor ATP

proof_lines: isoform AK2 mutant G54A, in 100 mM Tris/HCl (pH 8.0), at 25°C

proof_lines: isoform AK2 mutant G54A, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C

proof_lines: isoform AK2 mutant G54A, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C

enzyme: arginine kinase

substrate: L-Arg


proof_lines: isoform AK2 mutant L64V, in 100 mM Tris/HCl (pH 8.0), at 25°C

proof_lines: isoform AK2 mutant L64V, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C

proof_lines: isoform AK2 mutant L64V, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C


organism: Homo sapiens

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP


proof_lines: pH 8.0, 37°C, recombinant wild-type enzyme

proof_lines: wild type thymidine kinase-2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C

proof_lines: pH 7.5, recombinant wild-type enzyme, in absence of ATP


substrate_name: ganciclovir

assay_conditions_summary: pH 7.6; 310.15 K; cofactor ATP

proof_lines:
37°C, wild-type
pH 7.6, 37°C, wild-type
pH 7.2, wild-type

substrate: ganciclovir


proof_lines:
37°C, mutant SR39
37°C, mutant SR11
37°C, mutant SR26


organism: Cobetia marina

proof_lines: mutant G168K, pH 9.3, temperature not specified in the publication


proof_lines: mutant G168R, pH 9.3, temperature not specified in the publication


substrate_name: 4-nitrophenyl phosphate

proof_lines: wild-type enzyme, pH 8.0, 25°C, in absence of a phosphoryl group acceptor

substrate: 4-nitrophenyl phosphate


proof_lines:
25°C, pH 10.5, mutant enzyme T81A
25°C, pH 8, mutant enzyme T81A


substrate_name: p-nitrophenyl phosphate

proof_lines: mutant enzyme D369N, in absence of a phosphate acceptor

proof_lines: mutant enzyme D369N, in presence of a phosphate acceptor

proof_lines: mutant D369N, pH 8.0, 25°C, in presence of a phosphoryl group acceptor

substrate: p-nitrophenyl phosphate


proof_lines: mutant enzyme D369A, in absence of a phosphate acceptor


organism: Neurospora crassa

proof_lines: 37°C, pH 9.5, wild-type enzyme, 2 mM MgCl2

proof_lines: 37°C, pH 9.5, wild-type enzyme, without MgCl2


proof_lines: 37°C, pH 9.5, mutant os-1 enzyme, without MgCl2

proof_lines: 37°C, pH 9.5, mutant os-1 enzyme, 2 mM MgCl2


organism: Thermus yunnanensis


enzyme_display_name: alpha-amylase

organism: Bacillus sp. (in: firmicutes)

substrate_name: soluble starch

proof_lines:
mutant BACDELTANC, pH 8.0, 60°C
mutant BACDELTANC/DELTARS, pH 8.0, 60°C

enzyme: alpha-amylase

substrate: soluble starch


proof_lines: mutant BACDELTANC/DELTARS/M231L, pH 8.0, 60°C

proof_lines: mutant BACDELTANC/DELTARS/M231L/DELTAC31, pH 8.0, 60°C


organism: Thermoactinomyces vulgaris

substrate_name: starch

substrate: starch


assay_conditions_summary: pH 4.6; 310.15 K; buffer 0.1 M sodium citrate

proof_lines: wild type enzyme, in 0.1 M sodium citrate buffer (pH 4.6) at 37°C


organism: Bacillus subtilis

proof_lines: pH 6.5, 55°C, xylanase XynA, calculated as D-xylose liberated from 1,4-beta-D-xylan per second

proof_lines: pH 7.5, 70°C, chimeric enzyme CorA-XynAG3, calculated as D-xylose liberated from 1,4-beta-D-xylan per second

proof_lines: pH 6.5, 65°C, chimeric enzyme CorA-XynA, calculated as D-xylose liberated from 1,4-beta-D-xylan per second


proof_lines: pH 7.5, 70°C, thermostable xylanase variant XynAG3, calculated as D-xylose liberated from 1,4-beta-D-xylan per second


organism: Penicillium griseofulvum

substrate_name: arabinoxylan

proof_lines: pH 4.5, 30°C, mutant DELTAP130/K131S/K132S

substrate: arabinoxylan


organism: Thermomyces lanuginosus

substrate_name: oat spelt xylan

substrate: oat spelt xylan


organism: Thermobifida fusca

substrate_name: xylan

substrate: xylan


substrate_name: L-arginine

mutation_signature: truncated mutant enzyme

mutation_type: truncation

substrate: L-arginine


proof_lines:
wild-type enzyme, pH 9.5
37°C, pH 9.0, wild-type
wild type


substrate_name: L-glutamine

substrate: L-glutamine


substrate_name: hydroxylamine

proof_lines: wild-type, 25°C, pH not specified in the publication

substrate: hydroxylamine


substrate_name: L-Glu

proof_lines:
oxidized mutant enzyme E165C
reduced mutant enzyme E165C

substrate: L-Glu


organism: Phaseolus vulgaris

substrate_name: L-glutamate

substrate: L-glutamate


proof_lines: wild-type and mutant enzyme, pH 7.5, 37°C


enzyme_display_name: uronate dehydrogenase

organism: Agrobacterium tumefaciens

substrate_name: D-glucuronic acid

proof_lines:
wild type enzyme, at pH 8.0 and 30°C
wild type enzyme, at pH 8.0 and 25°C

enzyme: uronate dehydrogenase

substrate: D-glucuronic acid


substrate_name: beta-D-glucuronate

proof_lines: mutant enzyme A41P/H101N/H236K, at pH 8.0 and 25°C

substrate: beta-D-glucuronate


proof_lines: mutant enzyme A41P/H101Y/H236K, at pH 8.0 and 25°C


proof_lines: mutant enzyme A41P/H101Y/H236R, at pH 8.0 and 25°C


proof_lines: mutant enzyme A41P/H101N/H236R, at pH 8.0 and 25°C


proof_lines: mutant enzyme H101N/H236K, at pH 8.0 and 25°C


proof_lines: mutant enzyme A41P/H236K, at pH 8.0 and 25°C


proof_lines: mutant enzyme H101Y/H236K, at pH 8.0 and 25°C


enzyme_display_name: isocitrate dehydrogenase (NAD(+))

substrate_name: NAD+

assay_conditions_summary: pH 7.2; 298.15 K; cofactor NAD+

proof_lines: 25°C, pH 7.2, alpha-subunit mutant enzyme D230C

proof_lines: D230C, alpha-subunit mutant, in 20 mM isocitrate and 1 mM MnSO4

proof_lines: D230C, alpha-subunit mutant, in 20 mM isocitrate and 1mM MnSO4

enzyme: isocitrate dehydrogenase (NAD(+))

substrate: NAD+


proof_lines: 25°C, pH 7.2, alpha-subunit mutant enzyme D234C

proof_lines: D234C, alpha-subunit mutant, in 20 mM isocitrate and 1 mM MnSO4

proof_lines: D234C, alpha-subunit mutant, in 20 mM isocitrate and 1mM MnSO4


proof_lines: 25°C, pH 7.2, alpha-subunit mutant enzyme D206N

proof_lines: D206N, alpha-subunit mutant, in 20 mM isocitrate and 1 mM MnSO4

proof_lines: D206N, alpha-subunit mutant, in 20 mM isocitrate and 1mM MnSO4


proof_lines: 25°C, pH 7.2, beta-subunit mutant enzyme D217N

proof_lines: D217N, beta-subunit mutant, in 20 mM isocitrate and 1 mM MnSO4

proof_lines: D217N, beta-subunit mutant, in 20 mM isocitrate and 1mM MnSO4


substrate_name: isocitrate

proof_lines: 25°C, pH 7.2, gamma-subunit mutant enzyme D215N

proof_lines: D215N, gamma-subunit mutant, in 20 mM isocitrate and 1 mM MnSO4

proof_lines: D215N, gamma-subunit mutant, in 20 mM isocitrate and 1mM MnSO4

substrate: isocitrate


proof_lines: wild type enzyme in 20 mM isocitrate and 1 mM MnSO4

proof_lines: pH 7.2, 25°C, recombinant wild-type enzyme


organism: Streptococcus mutans

substrate_name: DL-isocitrate

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NAD+

proof_lines: pH 7.5, 37°C, recombinant enzyme mutant S102A

substrate: DL-isocitrate


proof_lines: pH 7.5, 37°C, recombinant enzyme mutant S102Y


proof_lines: pH 7.5, 37°C, recombinant enzyme mutant S102G


proof_lines: pH 7.5, 37°C, recombinant wild-type enzyme


proof_lines: pH 7.5, 37°C, recombinant enzyme mutant S102T


organism: Saccharomyces cerevisiae

substrate_name: D-isocitrate

proof_lines: octameric enzyme mutant IDH1/IDH2C56S/C150S/C242S with AMP, without DTT, pH 7.4, 24°C

proof_lines: octameric enzyme mutant IDH1/IDH2C56S/C150S/C242S with AMP and DTT, pH 7.4, 24°C

proof_lines: octameric enzyme mutant IDH1/IDH2C56S/C150S/C242S without AMP, with or without DTT, pH 7.4, 24°C

substrate: D-isocitrate


proof_lines: octameric enzyme mutant IDH1/IDH2C150S with AMP, with or without DTT, pH 7.4, 24°C

proof_lines: octameric enzyme mutant IDH1/IDH2C150S without AMP, with DTT, pH 7.4, 24°C

proof_lines: octameric enzyme mutant IDH1/IDH2C150S without AMP and DTT, pH 7.4, 24°C


proof_lines: octameric wild-type enzyme with AMP and DTT, pH 7.4, 24°C

proof_lines: octameric wild-type enzyme with AMP, without DTT, pH 7.4, 24°C

proof_lines: octameric wild-type enzyme without AMP and DTT, pH 7.4, 24°C


proof_lines: octameric enzyme mutant IDH1/IDH2C56S/C242S without AMP, with DTT, pH 7.4, 24°C

proof_lines: octameric enzyme mutant IDH1/IDH2C56S/C242S with AMP and DTT, pH 7.4, 24°C

proof_lines: octameric enzyme mutant IDH1/IDH2C56S/C242S with AMP, without DTT, pH 7.4, 24°C


organism: Mycobacterium tuberculosis

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NADH


substrate_name: NADH

substrate: NADH


organism: Cereibacter sphaeroides


assay_conditions_summary: pH 6.5; 303.15 K; cofactor NADH


organism: Streptomyces maritimus

substrate_name: Unknown

substrate: Unknown


substrate_name: L-Phe

substrate: L-Phe


organism: Escherichia coli

assay_conditions_summary: cofactor NADP+


substrate_name: NADP+

substrate: NADP+


organism: Homo sapiens

proof_lines: purified recombinant mutant beta-R99Q, pH 7.2, 25°C, in presence of 1 mM ADP


proof_lines: purified recombinant mutant gamma-R97Q, pH 7.2, 25°C, in presence of 1 mM ADP


enzyme_display_name: vanillyl-alcohol oxidase

organism: Penicillium simplicissimum

substrate_name: eugenol

enzyme: vanillyl-alcohol oxidase

substrate: eugenol


organism: Providencia vermicola

assay_conditions_summary: pH 8; 308.15 K; cofactor ADP

proof_lines: wild type enzyme, ADP bound to Na+, at pH 8.0 and 35°C


substrate_name: ADP

proof_lines: mutant enzyme S45A, ADP bound to Na+, at pH 8.0 and 35°C

substrate: ADP


organism: Synechocystis sp.

assay_conditions_summary: pH 7; 303.15 K; buffer 100 mM phosphate

proof_lines: catalase activity, wild type enzyme, in 100 mM phosphate buffer, pH 7.0, at 23°C

proof_lines: catalase activity, wild type enzyme, in 50 mM phosphate buffer, pH 7.0, and 30°C


enzyme_display_name: peroxidase

organism: Capsicum annuum

assay_conditions_summary: pH 5; 298.15 K; buffer 50 mM sodium citrate

proof_lines: in 50 mM sodium citrate buffer (pH 5.0), at 25°C

enzyme: peroxidase


enzyme_display_name: unspecific peroxygenase

organism: Chaetomium globosum

substrate_name: oleic acid

proof_lines: pH and temperature not specified in the publication

enzyme: unspecific peroxygenase

substrate: oleic acid


organism: Coprinellus radians

substrate_name: Naphthalene

assay_conditions_summary: pH 7; 298.15 K; buffer sodium phosphate-citrate

proof_lines: in sodium phosphate-citrate buffer at pH 4.5 and 25°C

proof_lines: in the presence of 5 mM benzyl alcohol, in sodium phosphate-citrate buffer at pH 7.0 and 25°C

substrate: Naphthalene


organism: Coprinopsis cinerea

substrate_name: Cyclohexanol

proof_lines: at pH 7.0, temperature not specified in the publication

substrate: Cyclohexanol


organism: Cyclocybe aegerita

substrate_name: Pyridine

assay_conditions_summary: pH 7; 295.15 K; buffer 10 mM phosphate

proof_lines: in 10 mM phosphate buffer (pH 7.0), temperature not specified in the publication

proof_lines: recombinant enzyme from Saccharomyces cerevisiae, at pH 7.0, temperature not specified in the publication

substrate: Pyridine


organism: Marasmius rotula


organism: Marasmius wettsteinii

substrate_name: cortisone

substrate: cortisone


organism: Mycothermus thermophilus

substrate_name: isophorone

substrate: isophorone


enzyme_display_name: tetracenomycin-F1 monooxygenase

organism: Streptomyces glaucescens

substrate_name: tetracenomycin F1

sequence_source: UniProtKB P39889 canonical TcmH entry (direct scalar metadata check)

enzyme: tetracenomycin-F1 monooxygenase

substrate: tetracenomycin F1


organism: Methylophaga aminisulfidivorans

substrate_name: Methimazole

substrate: Methimazole


organism: Rhodococcus jostii

substrate_name: Cyclobutanone

assay_conditions_summary: pH 8; 293.15 K; cofactor NADPH

proof_lines: with bicyclo[3.2.0]hept-2-en-6-one as cosubstrate, at pH 8.0 and 20°C

substrate: Cyclobutanone


enzyme_display_name: deacetoxycephalosporin-C synthase

organism: Amycolatopsis lactamdurans

substrate_name: oxoglutarate

enzyme: deacetoxycephalosporin-C synthase

substrate: oxoglutarate


organism: Hapsidospora chrysogena


organism: Streptomyces clavuligerus

proof_lines:
native enzyme
recombinant enzyme


enzyme_display_name: formate dehydrogenase

organism: [Candida] boidinii

assay_conditions_summary: pH 5.5; 303.15 K; buffer 100 mM potassium phosphate; cofactor NAD+

proof_lines: pH 5.5, temperature not specified in the publication

proof_lines: with HCO3- as cosubstrate, pH 7.0, temperature not specified in the publication

proof_lines: in 100 mM potassium phosphate buffer at pH 7.5, at 30°C

enzyme: formate dehydrogenase


organism: Ogataea angusta

substrate_name: S-formylglutathione

assay_conditions_summary: cofactor NAD+

substrate: S-formylglutathione


organism: Thermothelomyces thermophilus

assay_conditions_summary: pH 6; 298.15 K; cofactor NAD+

proof_lines: pH 6.0, temperature not specified in the publication

proof_lines: in the absence of metal ion, at pH 6.5 and 25°C

proof_lines: in the presence of 0.01 mM Mo6+, at pH 6.5 and 25°C


organism: Anabaena cylindrica

substrate_name: NADPH

proof_lines: NADPH-2,6-dichlorophenol indophenol diaphorase activity

proof_lines: ferredoxin-dependent cytochrome c reductase activity

substrate: NADPH


organism: Anabaena sp.

assay_conditions_summary: cofactor NADPH

proof_lines: electron transfer via the enzyme to oxidized ferredoxin and further to cytochrome c

proof_lines: electron transfer via the enzyme to K3Fe(CN)6


organism: Chlamydomonas reinhardtii

substrate_name: reduced ferredoxin

assay_conditions_summary: pH 8; 303.15 K; cofactor NADPH

proof_lines:
native enzyme, pH 8.0, 30°C
recombinant enzyme, pH 8.0, 30°C

substrate: reduced ferredoxin


organism: Chlorobaculum tepidum

assay_conditions_summary: pH 7; 298.15 K; buffer 2 mM ferricyanide in 20 mM HEPES-NaOH; cofactor NADPH

proof_lines: with 2 mM ferricyanide in 20 mM HEPES-NaOH buffer, at pH 7.0 and 25°C


organism: Euglena gracilis

substrate_name: iodonitrotetrazolium violet

proof_lines: 4 different fractions after ferredoxin-Sepharose chromatography

substrate: iodonitrotetrazolium violet


organism: Limnospira platensis

proof_lines: in a 2,6-dichlorophenolindophenol reduction assay


organism: Spinacia oleracea

substrate_name: dibromothymoquinone

assay_conditions_summary: pH 7; 288.15 K; cofactor NADPH

proof_lines: pH 7.0, 15°C, recombinant chimeric enzyme, diaphorase activity

substrate: dibromothymoquinone


organism: Sus scrofa


organism: Penicillium camemberti

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

proof_lines: with NADPH as cosubstrate, at pH 7.5 and 25°C

proof_lines: with NADP+ as cosubstrate, at pH 7.5 and 25°C


organism: Polaromonas naphthalenivorans

substrate_name: beta-D-galacturonate

assay_conditions_summary: pH 8; 298.15 K; cofactor NAD+

proof_lines:
pH 8.0, 25°C, method A
pH 8.0, 25°C, method B

substrate: beta-D-galacturonate


organism: Pseudomonas fluorescens


organism: Pseudomonas syringae

substrate_name: D-galacturonate

substrate: D-galacturonate


organism: Chromohalobacter israelensis


organism: Ectopseudomonas mendocina

proof_lines:
pH 8.0, 25°C, method B
pH 8.0, 25°C, method A


organism: Fulvimarina pelagi

substrate_name: beta-D-glucuronate

assay_conditions_summary: buffer conditions; cofactor NAD+

proof_lines: recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer

proof_lines: recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer

substrate: beta-D-glucuronate


organism: Oceanicola granulosus

substrate_name: D-Mannuronate

substrate: D-Mannuronate


enzyme_display_name: shikimate dehydrogenase (NADP+)

organism: Archaeoglobus fulgidus

substrate_name: NAD+

assay_conditions_summary: pH 7.3; 360.15 K; cofactor NADP+

enzyme: shikimate dehydrogenase (NADP+)

substrate: NAD+


organism: Camellia sinensis

substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

substrate: NADP+


organism: Capsicum annuum

substrate_name: shikimic acid

substrate: shikimic acid


organism: Chlamydomonas reinhardtii

substrate_name: shikimate

proof_lines: pH and temperature not specified in the publication

substrate: shikimate


organism: Cucumis sativus


organism: Escherichia coli

assay_conditions_summary: pH 9; 293.15 K; cofactor NADP+

proof_lines:
with shikimate, pH 9.0, 20°C
with NADP+, pH 9.0, 20°C


organism: Helicobacter pylori

assay_conditions_summary: pH 8; 298.15 K; cofactor NADP+

proof_lines:
with NADP+, pH 8.0, 25°C
with shikimate, pH 8.0, 25°C


organism: Lathyrus oleraceus


organism: Streptomyces viridochromogenes

assay_conditions_summary: buffer recombinant His-tagged enzyme; cofactor NAD+


organism: Populus trichocarpa

substrate_name: L-quinate

proof_lines: mutant S275G/T318G, pH and temperature not specified in the publication

substrate: L-quinate


organism: Rhodopirellula baltica


organism: Selaginella moellendorffii


organism: Solanum lycopersicum


organism: Staphylococcus aureus

proof_lines: pH not specified in the publication, 25°C, with NADP+

proof_lines: pH not specified in the publication, 25°C, with shikimate


organism: Toxoplasma gondii

assay_conditions_summary: pH 8.8; cofactor NADP+

proof_lines: pH 8.8, temperature not specified in the publication


organism: Vigna mungo


enzyme_display_name: (3R)-2'-hydroxyisoflavanone reductase

organism: Medicago sativa

assay_conditions_summary: pH 6; 303.15 K; buffer 0.2 M sodium phosphate

proof_lines: in 0.2 M sodium phosphate buffer, pH 6.0, at 30°C

enzyme: (3R)-2'-hydroxyisoflavanone reductase


organism: Homo sapiens

assay_conditions_summary: pH 7.2; 298.15 K; cofactor NAD+

proof_lines: pH 7.2, 25°C, recombinant mutant betaY137E, in presence of 1 mM ADP

proof_lines: pH 7.2, 25°C, recombinant mutant betaY137F


assay_conditions_summary: pH 7.5; cofactor NAD+

proof_lines:
pH 7.5
membrane-associated ICDH


organism: Leishmania mexicana

assay_conditions_summary: pH 7.4; 310.15 K; cofactor NAD+


organism: Methylobacillus flagellatus

assay_conditions_summary: pH 8.5; 315.15 K; cofactor NAD+


organism: Azotobacter vinelandii

substrate_name: D-isocitrate

substrate: D-isocitrate


organism: Bos taurus

substrate_name: D,L-isocitrate

assay_conditions_summary: cofactor NAD+

substrate: D,L-isocitrate


organism: Colwellia maris


organism: Cupriavidus necator


organism: Methylococcus capsulatus

substrate_name: isocitrate

substrate: isocitrate


organism: Phaeodactylum tricornutum

proof_lines: in the presence of Mn2+, at pH 8.0 and 25°C

proof_lines: in the presence of Mg2+, at pH 8.0 and 25°C


organism: Pseudomonas sp.


organism: Pyrococcus horikoshii

assay_conditions_summary: pH 7.8; 343.15 K; cofactor NAD+

proof_lines: 6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1 mM NAD+

proof_lines: 6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1mM NAD+


organism: Yarrowia lipolytica

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NAD+


organism: Zymomonas mobilis

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NADP+

proof_lines: pH 7.5, 37°C, with Mn2+, recombinant enzyme

proof_lines: pH 7.5, 37°C, with Mg2+, recombinant enzyme


enzyme_display_name: pyranose oxidase

organism: Lenzites styracinus

substrate_name: D-glucose

enzyme: pyranose oxidase

substrate: D-glucose


organism: Phanerodontia chrysosporium

assay_conditions_summary: pH 8

proof_lines:
oxygen concentration 1.07 mM
at pH 8
oxygen concentration 0.25 mM


organism: Phlebiopsis gigantea

substrate_name: oxygen

substrate: oxygen


organism: Pseudarthrobacter siccitolerans

substrate_name: L-arabinose

proof_lines: with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C

proof_lines: with D-glucose as cosubstrate, at pH 6.5 and 37°C

proof_lines: with O2 as electron acceptor, at pH 6.5 and 37°C

substrate: L-arabinose


organism: Thauera aromatica

assay_conditions_summary: pH 6.2; 303.15 K; cofactor CoA


organism: Aspergillus nidulans

proof_lines:
pH 6.5, 30°C, recombinant enzyme
pH 8.0, 30°C, recombinant enzyme


organism: Aspergillus oryzae

substrate_name: ferricenium hexafluorophosphate

proof_lines:
pH 8.0, 30°C, recombinant enzyme
pH 6.5, 30°C, recombinant enzyme

substrate: ferricenium hexafluorophosphate


organism: Gloeophyllum sepiarium


organism: Irpex lacteus

substrate_name: L-sorbose

substrate: L-sorbose


organism: Trametes hirsuta


organism: Trametes ochracea

proof_lines: soluble enzyme, 500 mM glucose used as electron acceptor, pH 5.0

proof_lines: pH 7.5, substrate D-glucose, measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C

proof_lines: pH 8.5, substrate D-glucose, measuring oxygen consumption (computer-interfaced Oxy-32 oxygen-monitoring system), 30°C


assay_conditions_summary: pH 7; 277.15 K; buffer conditions

proof_lines: mutant enzyme T169A, in 50 mM sodium phosphate (pH 7.0), at 4°C

proof_lines: mutant enzyme T169A, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C

proof_lines: mutant enzyme T169A, using D-galactose as cosubstrate,in 50 mM sodium phosphate (pH 7.0), at 25°C


proof_lines: mutant enzyme N593C, at pH 6.5, temperature not specified in the publication


organism: Trametes versicolor

substrate_name: alpha-D-glucose

substrate: alpha-D-glucose


organism: Tricholoma matsutake

substrate_name: D-galactose

substrate: D-galactose


organism: Penicillium simplicissimum


organism: Pseudomonas sp. 11/12A

substrate_name: Vanillyl alcohol

substrate: Vanillyl alcohol


organism: Arabidopsis thaliana

substrate_name: crotonyl-CoA

assay_conditions_summary: pH 7.2; 298.15 K; cofactor CoA

proof_lines: in 10 mM sodium phosphate, pH 7.2, at 25°C

substrate: crotonyl-CoA


organism: Brassica napus

substrate_name: crotonyl-[acyl-carrier protein]

assay_conditions_summary: cofactor NADH

substrate: crotonyl-[acyl-carrier protein]


organism: Carthamus tinctorius

proof_lines:
reductase I
reductase II


organism: Eimeria tenella

substrate_name: NADH

assay_conditions_summary: pH 7.5; cofactor NADH

substrate: NADH


organism: Haemophilus influenzae

substrate_name: Unknown

substrate: Unknown


organism: Persea americana

substrate_name: Crotonyl-N-acetyl-cysteamine

substrate: Crotonyl-N-acetyl-cysteamine


organism: Plasmodium falciparum

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADH

proof_lines:
pH 7.5, 25°C
luteolin
(-)-catechin gallate


organism: Staphylococcus aureus


enzyme_display_name: phenylalanine dehydrogenase

organism: Brevibacterium sp.

substrate_name: phenylpyruvate

enzyme: phenylalanine dehydrogenase

substrate: phenylpyruvate


organism: Dietzia maris

substrate_name: deamino-NAD+

substrate: deamino-NAD+


organism: Lysinibacillus sphaericus

substrate_name: L-leucine

substrate: L-leucine


assay_conditions_summary: pH 8.5; 298.15 K; buffer 50mM pH 8; cofactor NADH

proof_lines: mutant enzyme N145A, at 25°C inTris buffer 50mM pH 8.5, with NADH (0.1 mM), KCl (100 mM) and NH4Cl (400 mM)


organism: Streptococcus pneumoniae

assay_conditions_summary: pH 6.5; 303.15 K; cofactor NADH

proof_lines: 30°C, pH 6.5, in the absence of 100 mM NH4+

proof_lines: 30°C, pH 6.5, in the presence of 100 mM NH4+


organism: Vibrio cholerae serotype O1

assay_conditions_summary: pH 7; buffer 0.1 M sodium phosphate; cofactor NADPH

proof_lines: native enzyme, in 0.1 M sodium phosphate buffer (pH 7)


organism: Microbacterium sp.


organism: Nocardia sp.

substrate_name: NAD+

substrate: NAD+


organism: Pseudobacillus badius

substrate_name: L-tyrosine

assay_conditions_summary: pH 10.4; 298.15 K; buffer pH 10; cofactor NAD+

proof_lines: native enzyme, at 25°C, in 100 mM glycine/KCl/KOH buffer pH 10.4

substrate: L-tyrosine


organism: Rhodococcus sp. (in: high G+C Gram-positive bacteria)

substrate_name: L-Met

substrate: L-Met


organism: Sporosarcina ureae

substrate_name: L-phenylalanine

assay_conditions_summary: pH 10.4; 298.15 K; cofactor NAD+

proof_lines:
pH 10.4, 25°C, native enzyme
pH 10.4, 30°C, native enzyme
pH 10.4, 25°C, immobilized enzyme

substrate: L-phenylalanine


organism: Thermoactinomyces intermedius

substrate_name: L-Leu

substrate: L-Leu


enzyme_display_name: dihydrolipoyl dehydrogenase

organism: Acetivibrio thermocellus

enzyme: dihydrolipoyl dehydrogenase


organism: Ascaris suum

proof_lines: NADH:2,6-dichlorophenol indophenol reductase activity


organism: Bos taurus

assay_conditions_summary: pH 7.6; 310.15 K; buffer Tris-HCl

proof_lines: in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C


organism: Clostridium cylindrosporum

assay_conditions_summary: cofactor NADP+


organism: Clostridium kluyveri

substrate_name: nitrotetrazolium blue

assay_conditions_summary: cofactor NADPH

proof_lines:
recombinant DiaA
native DiaA

substrate: nitrotetrazolium blue


organism: Escherichia coli

substrate_name: dihydrolipoamide

assay_conditions_summary: cofactor NAD+

substrate: dihydrolipoamide


organism: Halobacterium salinarum

proof_lines:
reaction with lipoic acid
reaction with lipoamide


organism: Lathyrus oleraceus

substrate_name: (R,S)-lipoamide

proof_lines:
chloroplastic enzyme
mitochondrial enzyme

substrate: (R,S)-lipoamide


organism: Leishmania donovani

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NAD+

proof_lines:
enzyme variant 1, at pH 7.5 and 37°C
enzyme variant 2, at pH 7.5 and 37°C


organism: Moorella thermoacetica ATCC 39073


organism: Mycobacterium tuberculosis

proof_lines:
pH 7.5, 25°C
reaction with thio-NADH
reaction with NADH


organism: Mycolicibacterium smegmatis


organism: Natronobacterium gregoryi


organism: Peptostreptococcus glycinophilus


organism: Rattus norvegicus


organism: Saccharomyces cerevisiae


organism: Spinacia oleracea

substrate_name: lipoic acid

substrate: lipoic acid


organism: Starkeyomyces koorchalomoides

substrate_name: alpha-lipoamide

proof_lines: in 0.1 mM Tris-HCl (pH 7.0), 0.0005 mM EDTA, 0.01 mM beta2-mercaptoethanol, at 37°C

substrate: alpha-lipoamide


organism: Streptococcus pneumoniae

proof_lines: recombinant DLDH expressed without lipoic acid

proof_lines: recombinant DLDH expressed without without a lipoyl protein domain


organism: Streptomyces seoulensis


enzyme_display_name: sulfite oxidase

organism: Arabidopsis thaliana

substrate_name: sulfite

enzyme: sulfite oxidase

substrate: sulfite


organism: Capra hircus

substrate_name: ferricyanide

substrate: ferricyanide


organism: Deinococcus radiodurans


organism: Gallus gallus

substrate_name: cytochrome c

substrate: cytochrome c


organism: Sus scrofa

substrate_name: ubiquinone

assay_conditions_summary: pH 7.5; 310.15 K; buffer 50 mM Tris-HCl

proof_lines:
pH 7.5, 37°C, in absence of Zn2+
pH 7.5, 37°C, in presence of Zn2+
nitric oxide reduction, pH 7.5

substrate: ubiquinone


organism: Syntrophotalea carbinolica

substrate_name: NADH

substrate: NADH


organism: Thermoplasma acidophilum


organism: Trypanosoma cruzi


organism: Homo sapiens

assay_conditions_summary: pH 8.5; 298.15 K; buffer conditions

proof_lines: 25°C, pH 8.5, 100 mM buffer, mutant enzyme Y343F

proof_lines: 25°C, pH 9.5, 100 mM buffer, mutant enzyme Y343F

proof_lines: 25°C, pH 8.0, 100 mM buffer, mutant enzyme Y343F


assay_conditions_summary: pH 6

proof_lines: mutant enzyme A208D in 20 mM Tris at pH 6.0

proof_lines: mutant enzyme A208D in 20 mM Tris at pH 8.5


organism: Malva sylvestris


organism: Mus musculus


organism: Nicotiana tabacum


organism: Rattus norvegicus


organism: Thermus thermophilus


enzyme_display_name: thymidylate synthase

organism: Crithidia fasciculata

enzyme: thymidylate synthase


enzyme_display_name: glycine hydroxymethyltransferase

organism: Euglena gracilis

substrate_name: L-serine

enzyme: glycine hydroxymethyltransferase

substrate: L-serine


organism: Geobacillus stearothermophilus

proof_lines:
only tetrameric form
only dimeric form


organism: Hydrogenobacter thermophilus

substrate_name: glycine

substrate: glycine


organism: Hyphomicrobium methylovorum

substrate_name: tetrahydrofolate

substrate: tetrahydrofolate


organism: Ovis aries


organism: Spinacia oleracea

substrate_name: Unknown

substrate: Unknown


organism: Methanocaldococcus jannaschii

substrate_name: L-Ser

substrate: L-Ser


organism: Methanospirillum hungatei

proof_lines: pH and temperature not specified in the publication


organism: Oryctolagus cuniculus

proof_lines:
recombinant enzyme
cytosolic enzyme
mitochondrial enzyme


enzyme_display_name: citrate synthase (unknown stereospecificity)

organism: Acinetobacter calcoaceticus subsp. anitratus

substrate_name: acetyl-CoA

assay_conditions_summary: cofactor CoA

proof_lines:
wild-type, + 0.1 M KCl
wild-type

substrate: acetyl-CoA


substrate_name: citrate

enzyme: citrate synthase (unknown stereospecificity)

substrate: citrate


organism: Agave americana


organism: Cupriavidus necator


organism: Dictyostelium discoideum


organism: Drosophila melanogaster

substrate_name: oxaloacetate

proof_lines:
versus oxaloacetate, competitive
versus acetyl-CoA, competitive
versus acetyl-CoA

substrate: oxaloacetate


organism: Escherichia coli

assay_conditions_summary: pH 7.8; 298.15 K; cofactor CoA

proof_lines: wild-type enzyme, in presence of 0.1 M KCl


proof_lines:
K168 acetylated variant, pH 7.8, 25°C
K295 acetylated variant, pH 7.8, 25°C
K283 acetylated variant, pH 7.8, 25°C


assay_conditions_summary: pH 8; 310.15 K; cofactor CoA


organism: Halobacterium salinarum

proof_lines:
minimum at 1 M KCl
above


organism: Haloferax volcanii


organism: Heliocidaris crassispina


organism: Hyphomicrobium sp.


organism: Komagataeibacter europaeus


organism: Lathyrus oleraceus


organism: Leishmania donovani

assay_conditions_summary: pH 8; cofactor CoA

proof_lines: with respect to acetyl-CoA, at pH 8.0, temperature not specified in the publication

proof_lines: with respect to oxaloacetate, at pH 8.0, temperature not specified in the publication


organism: Mesorhizobium ciceri

proof_lines:
in absence of KCl
in presence of 100 mM KCl


organism: Methanosarcina barkeri


organism: Methylorubrum extorquens


organism: Natronobacterium gregoryi


organism: Natronomonas pharaonis


organism: Nitrosomonas sp.


organism: Priestia megaterium


organism: Pseudomonas sp.

proof_lines:
CS II
CS I


organism: Sulfolobus acidocaldarius


organism: Sus scrofa

assay_conditions_summary: pH 7.5; cofactor CoA


organism: Talaromyces trachyspermus

assay_conditions_summary: cofactor ATP

proof_lines:
in presence of 2.5 mM ATP
in presence of KCl
in presence of 5 mM ATP


organism: Tetrahymena pyriformis


organism: Pyrococcus furiosus


organism: Rhodothermus marinus

proof_lines:
hexameric enzyme form
dimeric enzyme form
dimeric enzyme form with propionyl-CoA


organism: Saccharolobus solfataricus

proof_lines: pH and temperature not specified in the publication, competitively inhibition versus acetyl-CoA

proof_lines: non-competitively inhibition versus oxaloacetate, pH and temperature not specified in the publication


organism: Acremonium sp.

substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


organism: Bacteria


organism: Escherichia coli


organism: Thermoplasma acidophilum


organism: Urocitellus richardsonii

proof_lines: purified enzyme from torpid skeletal muscle, at pH 8.0 and 8°C

proof_lines: purified enzyme from torpid skeletal muscle, at pH 8.0 and 37°C

proof_lines: purified enzyme from euthermic skeletal muscle, at pH 8.0 and 37°C


proof_lines: 5-enpolpyruvylshikimate 3-phosphate ketal formation by hydrolysis of radiolabelled 5-enpolpyruvylshikimate 3-phosphate, pH 7.0, 25°C

proof_lines: 5-enpolpyruvylshikimate 3-phosphate ketal formation in presence of shikimate 3-phosphate and 6.7 equivalents of phosphoenolpyruvate and 10 mM phosphate


proof_lines:
wild-type enzyme
wild-type and G96A mutant enzyme
pH 7.0, 30°C, wild-type EcaroA


organism: Isoptericola variabilis


organism: Klebsiella pneumoniae

proof_lines:
glyphosate-sensitive enzyme
glyphosate-resistant enzyme
in the presence of glyphosate


organism: Lathyrus oleraceus

substrate_name: phosphate

substrate: phosphate


organism: Neurospora crassa

proof_lines:
cosubstrate 3-phosphoshikimate
cosubstrate phosphoenolpyruvate


organism: Nicotiana sylvestris

proof_lines:
vs. phosphoenolpyruvate
vs. shikimate 3-phosphate


organism: Petunia x hybrida

proof_lines:
vs. phosphate
vs. 5-enolpyruvylshikimate-3-phosphate


organism: plant


organism: Salmonella enterica subsp. enterica serovar Typhimurium


organism: Sorghum bicolor


organism: Staphylococcus aureus

assay_conditions_summary: pH 7

proof_lines: pH 7.0, recombinant enzyme, in presence of 100 mM KCl


organism: Strobilanthes cusia


organism: Thermotoga maritima

proof_lines:
at pH 8.0 and 37°C
at pH 8.0 and 22°C


organism: Trichormus variabilis

substrate_name: 5-enolpyruvylshikimate 3-phosphate

substrate: 5-enolpyruvylshikimate 3-phosphate


enzyme_display_name: aspartate transaminase

organism: Bacillus sp. (in: firmicutes)

substrate_name: L-aspartate

enzyme: aspartate transaminase

substrate: L-aspartate


organism: Bos taurus


organism: Bubalus bubalis


organism: Chlamydomonas reinhardtii


organism: Spirodela polyrhiza

substrate_name: L-glutamate

assay_conditions_summary: pH 7.5; buffer 0.05 M potassium phosphate; cofactor NADH

proof_lines: cytoplasmic enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 0.9 mM L-aspartate, 2.7 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase

proof_lines: mitochondrial enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 1.6 mM L-aspartate, 4 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase

substrate: L-glutamate


organism: Trichomonas vaginalis

proof_lines:
30°C
30°C, substrate inhibition
30°C, competitive versus 2-oxoglutrate


organism: Escherichia coli

substrate_name: pyridoxyl 5'-phosphate

substrate: pyridoxyl 5'-phosphate


proof_lines: mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C


organism: Phormidium lapideum


organism: Saccharolobus solfataricus


enzyme_display_name: branched-chain-amino-acid transaminase

organism: Arabidopsis thaliana

enzyme: branched-chain-amino-acid transaminase


organism: Brevibacillus brevis

substrate_name: L-phenylalanine

proof_lines: pH 8.0, 37°C, leucine as amino group donor

substrate: L-phenylalanine


organism: [Brevibacterium] flavum


organism: Enterobacter sp.


organism: Entodinium sp.

substrate_name: L-isoleucine

proof_lines: no further commentaries on experimental conditions

substrate: L-isoleucine


substrate_name: L-tryptophan

proof_lines:
pH 8.0, 25°C
pH 8.0, 37°C
pH 8.0, 25°C, substrate L-phenylalanine

substrate: L-tryptophan


organism: Gluconobacter oxydans

substrate_name: L-norleucine

proof_lines: pH 8.3, 37°C, 4-methyl-2-oxopentanoate as amino group acceptor

proof_lines: pH 8.0, 37°C, isoleucine as amino group donor

substrate: L-norleucine


organism: Helicobacter pylori

assay_conditions_summary: pH 8; 310.15 K; buffer 50mM Tris-HCl

proof_lines: in 50mM Tris-HCl buffer, pH 8.0, at 37°C


organism: Hordeum vulgare

proof_lines: pH 8.4, 30°C, glutamate as amino group donor

proof_lines: pH 8.4, 30°C, L-valine as amino group donor


organism: Methanococcus aeolicus

proof_lines: pH and temperature not specified in the publication, cosubstrate: L-isoleucine

proof_lines: pH and temperature not specified in the publication, cosubstrate: L-valine

proof_lines: pH and temperature not specified in the publication, cosubstrate: L-phenylalanine


organism: Mycobacterium tuberculosis

substrate_name: L-leucine

proof_lines: cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C

proof_lines: cosubstrate 2-oxoglutarate, pH 7.4, 37°C

substrate: L-leucine


organism: Pseudomonas sp.

proof_lines: pH 8.0, 30°C, activated with beta-mercaptoethanol


organism: Rattus norvegicus

proof_lines: mitochondria of AS-30D cell, pH 7.8, 37°C


organism: Solanum lycopersicum

substrate_name: L-valine

proof_lines: SlBCAT2, pH not specified in the publication, 25°C

proof_lines: SlBCAT3, pH not specified in the publication, 25°C

substrate: L-valine


organism: Solanum pennellii

proof_lines: SpBCAT1, pH not specified in the publication, 25°C


organism: Sus scrofa

proof_lines:
pH 8.6, 25°C, heart enzyme
pH 8.6, 25°C
pH 8.2, 37°C, brain enzyme


organism: Sparus aurata

substrate_name: beta-D-fructose 2,6-bisphosphate

assay_conditions_summary: pH 7.4; 303.15 K; cofactor ATP

proof_lines:
pH 7.4, 30°C, in presence of phosphate
pH 7.5, 30°C, in presence of phosphate
pH 7.4, 30°C, presence of phosphate

substrate: beta-D-fructose 2,6-bisphosphate


organism: Spinacia oleracea

substrate_name: ATP

assay_conditions_summary: pH 8; 303.15 K; cofactor ATP

proof_lines: pH 8.0, forms 1-3, kinetic data of various enzyme forms

substrate: ATP


organism: Escherichia coli

substrate_name: Unknown

assay_conditions_summary: pH 8.2; 303.15 K; cofactor ATP

proof_lines:
pH 8.2, 30°C, mutant strain DF903
pH 8.2, 30°C, mutant strain DF905

substrate: Unknown


organism: Homo sapiens

substrate_name: beta-D-fructose 6-phosphate

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

proof_lines:
pH 7.8, absence of phosphate
pH 7.5, 30°C
pH 7.8

substrate: beta-D-fructose 6-phosphate


organism: Mus musculus


organism: Mytilus galloprovincialis


enzyme_display_name: thymidine kinase

organism: Bos taurus

assay_conditions_summary: pH 7; 310.15 K; cofactor ATP

enzyme: thymidine kinase


organism: Canid alphaherpesvirus 1


organism: Chlamydomonas reinhardtii

assay_conditions_summary: pH 8.5; 303.15 K; cofactor ATP


organism: Cryptosporidium parvum

assay_conditions_summary: pH 7.8; 298.15 K; cofactor ATP


organism: Felid alphaherpesvirus 1


organism: Herpes simplex virus

substrate_name: thymidine

substrate: thymidine


assay_conditions_summary: pH 7.6; 310.15 K; cofactor ATP


organism: Aspergillus niger


organism: Columba sp.

proof_lines:
pH 7.1, 30°C, phosphorylated enzyme
pH 7.1, 30°C, native pigeon enzyme
pH 7.1, 30°C, native enzyme


substrate_name: D-fructose 6-phosphate

proof_lines:
mutant enzyme E190Q, 1 mM Mg2+
mutant enzyme E190Q, 30 mM Mg2+

substrate: D-fructose 6-phosphate


substrate_name: deoxyuridine

proof_lines: determined by capillary electrophoresis method

substrate: deoxyuridine


assay_conditions_summary: pH 7.6; 310.15 K; buffer and temperature conditions; cofactor ATP

proof_lines: in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C


organism: Human alphaherpesvirus 3

assay_conditions_summary: 310.15 K; cofactor ATP

proof_lines:
two active sites for ATP
ATP in form of MgATP2-
37°C


organism: Hymenolepis diminuta


organism: Orthopoxvirus vaccinia


organism: Shrimp white spot syndrome virus

assay_conditions_summary: pH 8; 298.15 K; cofactor ATP


organism: Tequatrovirus T4


organism: Trypanosoma brucei

substrate_name: deoxyinosine

assay_conditions_summary: pH 7.8; 310.15 K; cofactor ATP

proof_lines: full-length enzyme, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C

proof_lines: thymidine kinase domain 2, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C

substrate: deoxyinosine


enzyme_display_name: pyruvate kinase

organism: Lactococcus lactis

substrate_name: GDP

enzyme: pyruvate kinase

substrate: GDP


organism: Pelophylax ridibundus

substrate_name: ADP

assay_conditions_summary: pH 7.35; cofactor ADP

substrate: ADP


organism: Pigeon

assay_conditions_summary: cofactor ADP


organism: Sus scrofa

assay_conditions_summary: 298.15 K; cofactor ADP

proof_lines:
at 25°C
solubilized enzyme
bound enzyme


organism: Tenebrio molitor

substrate_name: phosphoenolpyruvate

proof_lines: in the presence of fructose 1,6-diphosphate

substrate: phosphoenolpyruvate


organism: Zymomonas mobilis

assay_conditions_summary: pH 6.5; 298.15 K; cofactor ADP


enzyme_display_name: acetate kinase

organism: Acetivibrio thermocellus

substrate_name: acetate

enzyme: acetate kinase

substrate: acetate


organism: Acholeplasma laidlawii

assay_conditions_summary: pH 7.4; 298.15 K; cofactor ATP


organism: Cereibacter sphaeroides

assay_conditions_summary: pH 5.7; cofactor ATP

proof_lines: with acetate as cosubstrate, at pH 5.7 and 37Â°C


organism: Clostridium acetobutylicum

substrate_name: acetyl phosphate

assay_conditions_summary: pH 7; 309.15 K; cofactor ATP

substrate: acetyl phosphate


organism: Escherichia coli


organism: Neomoorella thermoacetica


organism: Nitratidesulfovibrio vulgaris

assay_conditions_summary: pH 7.5; 298.15 K; cofactor ADP


organism: Propionibacterium freudenreichii


organism: Busycotypus canaliculatum

assay_conditions_summary: pH 7; 293.15 K; cofactor ADP

proof_lines: in the absence of fructose 1,6-diphosphate


organism: Chlamydomonas reinhardtii


organism: Cicer arietinum


organism: Entodinium sp.


organism: Veillonella parvula

assay_conditions_summary: pH 7.3; 310.15 K; cofactor ATP

proof_lines:
pH 7.3, 37°C, 50 M succinate
pH 7.3, 37°C
pH 7.3, 37°C, 0.2 M succinate


enzyme_display_name: creatine kinase

organism: Tetronarce californica

assay_conditions_summary: 303.15 K; cofactor ATP

enzyme: creatine kinase


organism: Urocitellus richardsonii

substrate_name: Creatine

assay_conditions_summary: 298.15 K; cofactor ATP

proof_lines:
25°C, myosin bound enzyme
25°C, hibernating squirrel
25°C, euthermic squirrel

substrate: Creatine


organism: Rhodopseudomonas palustris

substrate_name: UTP

substrate: UTP


organism: Salmonella enterica subsp. enterica serovar Typhimurium

assay_conditions_summary: pH 7.9; 310.15 K; cofactor ATP

proof_lines: acetate kinase EutP, at 40Â°C, pH not specified in the publication

proof_lines: acetate kinase EutQ, at 40Â°C, pH not specified in the publication


organism: Streptococcus pyogenes


organism: Thermotoga maritima

assay_conditions_summary: pH 7; 323.15 K; cofactor ATP


enzyme_display_name: adenylate kinase

organism: Ancylobacter novellus

substrate_name: AMP

proof_lines:
pH 7.5, 25°C, ADP production
pH 8.7, 25°C

enzyme: adenylate kinase

substrate: AMP


organism: Bos taurus

assay_conditions_summary: cofactor ATP


organism: Chlamydia pneumoniae


organism: Cyprinus carpio


enzyme_display_name: arginine kinase

organism: Ctenocephalides felis

substrate_name: L-Arg

proof_lines:
isoform arginine kinase 2
isoform arginine kinase 1

enzyme: arginine kinase

substrate: L-Arg


organism: Locusta migratoria manilensis

assay_conditions_summary: pH 8; 303.15 K; cofactor ATP

proof_lines: mutant enzyme I121K, in 100 mM Tris, pH 8.0, at 30°C


organism: Steinernema carpocapsae

assay_conditions_summary: pH 7.5; 299.15 K; cofactor ATP


organism: Sthenoteuthis oualaniensis

assay_conditions_summary: pH 7.2; 298.15 K; cofactor ATP


organism: Doryteuthis pealeii


organism: Escherichia coli

assay_conditions_summary: pH 7.4; 300.15 K; cofactor ATP

proof_lines:
cosubstrate ATP, pH 7.4, 27°C
cosubstrate 2-dAMP, 27°C, pH 7.4
pH 7.4, 27°C


organism: Geobacillus stearothermophilus

assay_conditions_summary: 310.15 K; cofactor ATP


organism: Halothiobacillus neapolitanus

assay_conditions_summary: pH 8.5; cofactor ADP

proof_lines:
pH 8.5
cosubstrate ATP, pH 8.5


organism: Mycobacterium tuberculosis


organism: Nicotiana tabacum


organism: Oryctolagus cuniculus

assay_conditions_summary: pH 8; 298.15 K; cofactor ATP


organism: Pseudomonas denitrificans (nom. rej.)

assay_conditions_summary: pH 7.6; 298.15 K; cofactor ADP


organism: Tritrichomonas suis

assay_conditions_summary: cofactor ADP


organism: Trypanosoma brucei


organism: Vibrio natriegens

assay_conditions_summary: pH 7.4; 303.15 K; cofactor ATP


organism: Zea mays

assay_conditions_summary: pH 7.8; 298.15 K; cofactor ATP

proof_lines:
cosubstrate MgADP-, pH 7.8, 25°C
cosubstrate AMP, pH 7.8, 25°C
ATP in form of MgATP2-


organism: Rattus norvegicus

assay_conditions_summary: pH 7; 310.15 K; cofactor ATP


organism: Schistosoma mansoni

assay_conditions_summary: pH 7.4; cofactor ATP

proof_lines: isoform ADK1, in 20 mM Tris-HCl (pH 7.4), 150 mM NaCl and 5 mM 2-mercaptoethanol, temperature not specified in the publication

proof_lines: isoform ADK2, in 20 mM Tris-HCl (pH 7.4), 150 mM NaCl and 5 mM 2-mercaptoethanol, temperature not specified in the publication


organism: Strongylocentrotus purpuratus

assay_conditions_summary: pH 7.6; 289.15 K; cofactor ADP

proof_lines: pH 7.6, 16°C, enzyme from embryonic cilia


organism: Sulfolobus acidocaldarius

assay_conditions_summary: 343.15 K; cofactor ATP


organism: Homo sapiens

substrate_name: nicotinamide ribonucleotide

assay_conditions_summary: pH 7.6; 310.15 K; cofactor NAD+

substrate: nicotinamide ribonucleotide


organism: Leishmania braziliensis

substrate_name: ATP

assay_conditions_summary: pH 7.4; 293.15 K; cofactor NAD+

substrate: ATP


organism: Saccharomyces cerevisiae

substrate_name: NAD+

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NAD+

substrate: NAD+


enzyme_display_name: acetylcholinesterase

organism: Locusta migratoria manilensis

substrate_name: acetylthiocholine

assay_conditions_summary: pH 7.5; 297.15 K; buffer 100 mM phosphate

proof_lines: in 100 mM phosphate buffer, pH 7.4, at 30°C

enzyme: acetylcholinesterase

substrate: acetylthiocholine


enzyme_display_name: alkaline phosphatase

organism: Anabaena sp.

substrate_name: 4-nitrophenyl phosphate

assay_conditions_summary: pH 8; 298.15 K; buffer the presence of phosphate acceptor (1 M Tris-HCl

proof_lines: pH 8, 25°C, in the presence of phosphate acceptor (1 M Tris-HCl buffer)

enzyme: alkaline phosphatase

substrate: 4-nitrophenyl phosphate


organism: Anticarsia gemmatalis


organism: Aspergillus caespitosus

proof_lines:
in presence of Mg2+
without Mg2+
with Mg2+


organism: Bacillus amyloliquefaciens


organism: Bacillus licheniformis

substrate_name: p-nitrophenyl phosphate

substrate: p-nitrophenyl phosphate


organism: Bacillus sp. OK-1


organism: Bos taurus

assay_conditions_summary: pH 9.8; 293.15 K; buffer 37°C; cofactor ATP

proof_lines: 37°C, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3,4,4-benzophenone tetracarboxylic dianhydride

proof_lines: 57°C, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3,4,4-benzophenone tetracarboxylic dianhydride


organism: Camelus bactrianus


organism: Entrophospora etunicata

substrate_name: glucose 6-phosphate

substrate: glucose 6-phosphate


organism: Escherichia coli

assay_conditions_summary: pH 10.5; 310.15 K; buffer MOPS buffer

proof_lines: conjugate between enzyme and ZZ protein, pH not specified in the publication, temperature not specified in the publication

proof_lines: native enzyme, pH not specified in the publication, temperature not specified in the publication

proof_lines: fusion protein with ZZ protein, pH not specified in the publication, temperature not specified in the publication


organism: Gallus gallus


organism: Geobacillus stearothermophilus


organism: Candida tropicalis

substrate_name: diphosphate

substrate: diphosphate


organism: Chlorocebus sabaeus

substrate_name: Phenyl phosphate

substrate: Phenyl phosphate


organism: Cyberlindnera jadinii


organism: Dioszegia sp.

proof_lines:
at pH 9.0 and 47°C
pH 9.0, 47°C


organism: Macaca mulatta


organism: Meriones unguiculatus


organism: Mesocricetus auratus


organism: Mus musculus


organism: Papio cynocephalus


organism: Paramecium tetraurelia


organism: Penaeus merguiensis


organism: Penicillium chrysogenum


organism: Neurospora crassa


organism: Onchocerca ochengi


organism: Oryctolagus cuniculus


organism: Pandalus borealis

proof_lines: 10°C, enzyme with aliphatic-group modification by 3,3,4,4-tetracarboxylic acid in a photo-assisted reaction


organism: Pinctada fucata


organism: Porphyromonas gingivalis

proof_lines:
pH 10.2, in presence of 1 mM Mn2+
pH 9.2, absence of divalent metal ions


organism: Rattus norvegicus

substrate_name: beta-Glycerophosphate

assay_conditions_summary: cofactor ATP

proof_lines:
soluble enzyme form
membranous enzyme form
enzyme from 20 day term fetal placentae

substrate: beta-Glycerophosphate


organism: Rhizopus microsporus


organism: Rhizopus microsporus var. microsporus

proof_lines:
pH 8.5, 45°C
presence of FeCl3, pH 8.5, 45°C


organism: Saccharomyces cerevisiae


organism: Scrobicularia plana

proof_lines: pH 8.5, 30°C, digestive gland and siphon


organism: Serratia marcescens

proof_lines: CAPase IAPase


organism: Synechococcus elongatus PCC 7942 = FACHB-805


organism: Terfezia claveryi


organism: Thermotoga maritima


organism: Thermotoga neapolitana


organism: Dosidicus gigas

assay_conditions_summary: 323.15 K; buffer the stated

proof_lines: in 40 mM sodium citrate buffer, 0.1 mM dithiothreitol, 110 mM NaCl, 20 mM MgCl2, and 20 mM CaCl2, at 50°C


organism: Escherichia coli

substrate_name: ADP

assay_conditions_summary: pH 7; 295.15 K; cofactor ATP

substrate: ADP


organism: Metamycoplasma arthritidis


organism: Rhipicephalus microplus

substrate_name: p-Nitrophenyl phenylphosphonate

substrate: p-Nitrophenyl phenylphosphonate


organism: Ulva pertusa


organism: Vibrio sp.


organism: Vibrio splendidus

proof_lines:
at pH 9.0 and 25°C
with 0.5 M NaCl, at pH 8.0 and 25°C
at pH 10.5 and 25°C


organism: Walterinnesia aegyptia

substrate_name: Unknown

substrate: Unknown


enzyme_display_name: alpha-amylase

organism: Anoxybacillus flavithermus

substrate_name: starch

enzyme: alpha-amylase

substrate: starch


organism: Bacillus amyloliquefaciens

substrate_name: soluble starch

substrate: soluble starch


organism: Bacillus sp. (in: firmicutes)

proof_lines: calculated as low molecular weight products


organism: Bacillus subtilis

substrate_name: maltopentaose

substrate: maltopentaose


organism: Geobacillus thermoleovorans


organism: Gracilibacillus dipsosauri


organism: Heterorhabditis bacteriophora

proof_lines:
pH 7.0, 40°C, alpha-amylase I
pH 7.0, 40 °C, alpha-amylase I


organism: Homo sapiens

substrate_name: alpha-maltotriosyl fluoride

proof_lines:
30°C, pH 7.0
commercial substrate, pH 7.0
pH 7.0, uncompetitive inhibition

substrate: alpha-maltotriosyl fluoride


organism: Corallococcus sp. EGB


organism: Fusicoccum sp.


organism: Fusidium sp.

substrate_name: maltohexaose

substrate: maltohexaose


organism: Geobacillus thermodenitrificans


proof_lines: pH 6.0, 37°C, the mutants Y151M and W58L exhibit reduced inhibitory efficiency with 92 and 97% remaining enzyme activity at 0.00235 mM inhibitor concentration, respectively, pH 6.0, 37°C


organism: Hyphopichia burtonii

substrate_name: maltotriose

substrate: maltotriose


organism: Lipomyces starkeyi

proof_lines: pH 6.0, 40°C, recombinant His-tagged enzyme


organism: Malus pumila


organism: Mesocentrotus nudus

substrate_name: maltohexaitol

substrate: maltohexaitol


organism: Moesziomyces antarcticus

proof_lines: short chain amylose, calculated as low molecular weight products

proof_lines: from rat liver, calculated as low molecular weight products


organism: Pseudoalteromonas haloplanktis


organism: Pseudomonas sp.


enzyme_display_name: phosphatidylcholine 12-monooxygenase

organism: Ricinus communis

enzyme: phosphatidylcholine 12-monooxygenase


enzyme_display_name: tryptophan synthase

organism: Thermotoga maritima

substrate_name: L-Serine; Indole

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

enzyme: tryptophan synthase

substrate: L-Serine; Indole


substrate_name: (3-Indolyl)-glycerol phosphate

substrate: (3-Indolyl)-glycerol phosphate


organism: Halobacterium salinarum

substrate_name: 4-nitrophenyl phosphate

proof_lines: pH 8.5, 40°C, enzyme in reversed micelles, 384 mM NaCl, without Mn2+

proof_lines: pH 8.5, 40°C, enzyme in reversed micelles, 680 mM NaCl, without Mn2+

proof_lines: pH 8.5, 40°C, enzyme in reversed micelles, 192 mM NaCl, 2.5 mM Mn2+

substrate: 4-nitrophenyl phosphate


organism: Helicoverpa armigera

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP


organism: Lepus townsendii


organism: Sus scrofa


organism: Pyrococcus furiosus


organism: Ruminobacter amylophilus

substrate_name: amylose

proof_lines:
micromol of D-glucosidic bond per liter
micromol of D-glucosidic bond per L

substrate: amylose


organism: Ruminococcus bromii

substrate_name: amylopectin

substrate: amylopectin


organism: Saitozyma flava


organism: Streptomyces fragilis


organism: Streptomyces megasporus


organism: Struthio camelus


organism: Thermoactinomyces vulgaris

substrate_name: pullulan

proof_lines: short chain amylose, as anhydro-glucose units

substrate: pullulan


organism: Vibrio sp.


organism: Vigna angularis


organism: Vigna radiata


organism: Zunongwangia profunda

proof_lines: pH and temperature not specified in the publication


enzyme_display_name: alpha-galactosidase

organism: Acinetobacter sp.

substrate_name: raffinose

proof_lines:
isoform Ag-II, at pH 7.0 and 36°C
isoform Ag-I, at pH 7.0 and 36°C

enzyme: alpha-galactosidase

substrate: raffinose


organism: Agroathelia rolfsii

substrate_name: 4-nitrophenyl alpha-D-galactopyranoside

substrate: 4-nitrophenyl alpha-D-galactopyranoside


organism: Arabidopsis thaliana

proof_lines: pH 7.5, temperature not specified in the publication


organism: Arachis hypogaea

proof_lines: competitive with p-nitrophenyl alpha-D-galactopyranoside


organism: Aspergillus ficuum

substrate_name: 2-nitrophenyl alpha-D-galactopyranoside

substrate: 2-nitrophenyl alpha-D-galactopyranoside


organism: Aspergillus flavipes

substrate_name: p-nitrophenyl alpha-D-galactopyranoside

substrate: p-nitrophenyl alpha-D-galactopyranoside


organism: Aspergillus nidulans

substrate_name: melibiose

substrate: melibiose


organism: Aspergillus terreus

substrate_name: 4-nitrophenyl alpha-D-galactoside

substrate: 4-nitrophenyl alpha-D-galactoside


organism: Bacillus sp. (in: firmicutes)


organism: Bifidobacterium breve


organism: Bifidobacterium longum subsp. Longum


organism: Bispora sp.


organism: Caldanaerobius polysaccharolyticus


organism: Carica papaya

substrate_name: p-nitrophenyl-alpha-D-galactoside

proof_lines:
alpha-galactosidase 3
alpha-galactosidase 2
alpha-galactosidase 1

substrate: p-nitrophenyl-alpha-D-galactoside


organism: Citrobacter freundii


organism: Cocos nucifera


organism: Coffea sp.

substrate_name: alpha-phenylgalactoside

proof_lines:
alpha-galactosidase II
alpha-galactosidase I

substrate: alpha-phenylgalactoside


organism: Corynebacterium murisepticum


organism: Debaryomyces hansenii

proof_lines:
pH 5.0, 55°C, intracellular enzyme
pH 5.0, 55°C
immobilized enzyme, at pH 5.0 and 40°C


organism: Diehliomyces microsporus

substrate_name: 4-nitrophenyl alpha-D-glucopyranoside

substrate: 4-nitrophenyl alpha-D-glucopyranoside


organism: Homo sapiens


substrate_name: ceramide trihexoside

proof_lines:
alpha-galactosidase B
alpha-galactosidase A

substrate: ceramide trihexoside


organism: Lens culinaris

substrate_name: p-nitrophenyl alpha-D-galactoside

proof_lines:
cell wall alpha-galactosidase I
cell wall alpha-galactosidase II
protein bodies alpha-galactosidase I

substrate: p-nitrophenyl alpha-D-galactoside


organism: Lupinus angustifolius

substrate_name: p-nitrophenyl alpha-D-galactose

proof_lines:
alpha-galactosidase I
alpha-galactosidase II

substrate: p-nitrophenyl alpha-D-galactose


enzyme_display_name: unspecific peroxygenase

organism: Coprinus radians

substrate_name: H2O2; Benzyl alcohol

enzyme: unspecific peroxygenase

substrate: H2O2; Benzyl alcohol


organism: Cyclocybe aegerita

proof_lines: mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication

proof_lines: mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 4.0, temperature not specified in the publication

proof_lines: mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication


assay_conditions_summary: pH 7

proof_lines: mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication

proof_lines: mutant enzyme G241D/R257K, at pH 4.0, temperature not specified in the publication

proof_lines: mutant enzyme G241D/R257K, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication


organism: Sulfurisphaera tokodaii

proof_lines:
pH 10, 30°C, wild-type enzyme
pH 7, 30°C, wild-type enzyme
pH 5, 30°C, wild-type enzyme


proof_lines:
pH 7, 30°C, mutant enzyme F310A/F320Q
pH 5, 30°C, mutant enzyme F310A/F320Q
pH 10, 30°C, mutant enzyme F310A/F320Q


organism: Achaetomiella virescens

substrate_name: Naphthalene

proof_lines: mutant enzyme F88L, at 25°C, pH not specified in the publication

substrate: Naphthalene


proof_lines: wild type enzyme, at 25°C, pH not specified in the publication


proof_lines: mutant enzyme T158F, at 25°C, pH not specified in the publication


substrate_name: benzyl alcohol

proof_lines: at 25°C, pH not specified in the publication

substrate: benzyl alcohol


enzyme_display_name: shikimate dehydrogenase (NADP+)

organism: Mycobacterium tuberculosis

substrate_name: NADPH

assay_conditions_summary: pH 9; 298.15 K; cofactor NADP+

proof_lines:
pH 9.0, 25°C
pH 9.0, 25°C, with NADP+
pH 7.0, 25°C, with 3-dehydroshikimate

enzyme: shikimate dehydrogenase (NADP+)

substrate: NADPH


organism: Nicotiana tabacum

substrate_name: shikimate

assay_conditions_summary: pH 9; 295.15 K; cofactor NADP+

substrate: shikimate


organism: Physcomitrium patens

proof_lines: pH and temperature not specified in the publication


organism: Pinus taeda


enzyme_display_name: arylamine N-acetyltransferase

organism: Columba sp.

substrate_name: serotonin

assay_conditions_summary: cofactor CoA

enzyme: arylamine N-acetyltransferase

substrate: serotonin


organism: Mycobacterium marinum

substrate_name: isoniazid

assay_conditions_summary: pH 8; 301.15 K; cofactor CoA

substrate: isoniazid


organism: Oryctolagus cuniculus


organism: Lysobacter enzymogenes

substrate_name: p-nitrophenyl phosphate

proof_lines:
and a second Km value of 0.056 mM
and a second Km value of 0.034 mM

substrate: p-nitrophenyl phosphate


organism: Escherichia coli


organism: Flavobacterium sp.


organism: Geobacillus stearothermophilus

assay_conditions_summary: pH 6

proof_lines:
alpha-galactosidase II
alpha-galactosidase II, pH 6
alpha-galactosidase I, pH 6


organism: Glycine max

proof_lines:
tetramer
monomer
enzyme form P2


organism: Micrococcus sp.


organism: Monascus pilosus


organism: Oryza sativa

substrate_name: digalactosyl diacylglycerol

substrate: digalactosyl diacylglycerol


organism: Penicillium canescens

substrate_name: 4-nitrophenyl alpha-D-galactopyranoside

substrate: 4-nitrophenyl alpha-D-galactopyranoside


organism: Phaseolus vulgaris


organism: Pleurotus floridanus

substrate_name: p-nitrophenyl alpha-D-galactopyranoside

substrate: p-nitrophenyl alpha-D-galactopyranoside


organism: Poterioochromonas malhamensis


organism: Prunus dulcis

substrate_name: melibiose

substrate: melibiose


organism: Rasamsonia emersonii


organism: Rhizopus sp.

proof_lines: in 0.1 M McIlvaine buffer (pH 4.8) at 37°C


organism: Saccharomyces pastorianus


organism: Saccharum officinarum

substrate_name: p-nitrophenyl-alpha-D-galactopyranoside

substrate: p-nitrophenyl-alpha-D-galactopyranoside


organism: Solanum lycopersicum

substrate_name: stachyose

proof_lines:
alpha-galactosidase II
alpha-galactosidase I

substrate: stachyose


organism: Stachys affinis


organism: Tachigali multijuga

substrate_name: 4-nitrophenyl alpha-D-galactoside

substrate: 4-nitrophenyl alpha-D-galactoside


organism: Talaromyces flavus


organism: Thermomyces lanuginosus

assay_conditions_summary: pH 4.5; 323.15 K; buffer 50 mM citrate

proof_lines: at 55°C, in 50 mM citrate buffer (pH 4.2)


organism: Thermotoga maritima


organism: Thermotoga neapolitana


organism: Trametes cinnabarina

substrate_name: p-nitrophenyl alpha-D-galactoside

substrate: p-nitrophenyl alpha-D-galactoside


organism: Trichoderma reesei


organism: Vicia faba

substrate_name: p-aminophenyl alpha-D-galactoside

substrate: p-aminophenyl alpha-D-galactoside


organism: Vigna unguiculata

substrate_name: raffinose

proof_lines:
alpha-galactosidase II1
alpha-galactosidase II2
alpha-galactosidase I

substrate: raffinose


organism: Yersinia pestis


enzyme_display_name: beta-fructofuranosidase

organism: Arabidopsis thaliana

substrate_name: sucrose

proof_lines:
enzyme form INV2
enzyme forms INV1 and INV3

enzyme: beta-fructofuranosidase

substrate: sucrose


organism: Arthrobacter globiformis


organism: Aspergillus fumigatus

proof_lines:
enzyme form F-2
enzyme form F-1


organism: Aspergillus nidulans


organism: Aspergillus niger

proof_lines: 37°C, pH not specified in the publication


organism: Aspergillus ochraceus

assay_conditions_summary: pH 5; 313.15 K; buffer sodium acetate

proof_lines: in sodium acetate buffer, 100 mM, pH 4.5, at 60°C


organism: Aspergillus parasiticus


organism: Aspergillus phoenicis


organism: Aureobasidium sp.


organism: Avena sativa

proof_lines:
invertase I
invertase II


organism: Azotobacter chroococcum


organism: Bacillus cereus


organism: Aspergillus tamarii

proof_lines: transfructosylation reaction, pH 7.4, 50°C


organism: Aspergillus terreus

assay_conditions_summary: pH 4.6; 328.15 K; buffer conditions

proof_lines: in 100 mM sodium acetate buffer, pH 5.0, at 50°C


organism: Aspergillus wentii


organism: Aureobasidium pullulans

assay_conditions_summary: pH 5; 303.15 K; buffer 50 mM sodium acetate

proof_lines: in 50 mM sodium acetate buffer (pH 5.0), at 30°C


organism: Beta vulgaris

proof_lines:
saline-released acid invertase
extracellular invertase I
acid invertases


organism: Bifidobacterium adolescentis


organism: Bifidobacterium breve

proof_lines: pH and temperature not specified in the publication


organism: Carica papaya


organism: Cicer arietinum


organism: Citrus sinensis


organism: Clostridium perfringens


organism: Corynebacterium glutamicum


organism: Corynebacterium murisepticum


organism: Cyberlindnera jadinii

proof_lines: native invertase, pre-incubation at 80°C for 20 min, 55°C, pH 4.5


organism: Escherichia coli


organism: Fusarium oxysporum

proof_lines:
invertase P-1
invertase P-2


organism: Fusarium solani

assay_conditions_summary: pH 5; 313.15 K; buffer MES


organism: Glycine max


organism: Helianthus tuberosus


organism: Hordeum vulgare

proof_lines:
invertase I
invertase IIA
invertase IIB


organism: Kluyveromyces marxianus

assay_conditions_summary: pH 5


organism: Lacticaseibacillus paracasei


organism: Lathyrus oleraceus

proof_lines: pH 6.5, 37°C, hyperbolic saturation kinetics


organism: Lilium auratum

proof_lines:
invertase 2
invertase 1


organism: Lilium longiflorum

proof_lines:
invertase I
invertase III
invertase II


organism: Limosilactobacillus reuteri


organism: Mangifera indica


organism: Meyerozyma guilliermondii


organism: Nicotiana tabacum


organism: Paecilomyces variotii


organism: Penicillium aurantiogriseum


organism: Penicillium citreonigrum


organism: Penicillium janczewskii


organism: Phaffia rhodozyma


organism: Phytophthora megasperma


organism: Prunus persica


organism: Pseudomonas fluorescens


organism: Raphanus sativus


organism: Rhizopus stolonifer


organism: Rhodotorula glutinis


organism: Ricinus communis


organism: Saccharomyces cerevisiae

proof_lines:
extracellular and intracellular enzyme
exteracellular enzyme
40°C, pH 5.5


organism: Schwanniomyces occidentalis

assay_conditions_summary: pH 5.6; 323.15 K; buffer 0.2M sodium acetate

proof_lines: in 0.2M sodium acetate buffer (pH 5.6), at 50°C


organism: Solanum lycopersicum

proof_lines: enzyme embedded within a polyacrylamide gel


organism: Termitomyces clypeatus


organism: Thermotoga maritima


organism: Thermotoga neapolitana


organism: Trametes sanguinea


organism: Tropaeolum majus


organism: Woodfordia fruticosa


organism: Zymomonas mobilis

proof_lines: immobilized enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5

proof_lines: free enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5.5


organism: Thermobacillus xylanilyticus


organism: Albifimbria verrucaria

substrate_name: carboxymethylcellulose

proof_lines:
soluble xylan from wheat straw
soluble xylan from oat spelts

substrate: carboxymethylcellulose


organism: Aspergillus fumigatus

substrate_name: birchwood xylan

substrate: birchwood xylan


organism: Aspergillus giganteus

substrate_name: xylan

proof_lines: xylanase II, oatspelt xylan, pH 6.0, 50°C

proof_lines: xylanase I, oatspelt xylan, pH 6.0, 50°C

proof_lines: xylanase I, birchwood xylan, pH 6.0, 50°C

substrate: xylan


organism: Aspergillus niger


organism: Acetivibrio thermocellus

substrate_name: 4-nitrophenyl alpha-L-arabinofuranoside

proof_lines:
at pH 5.0 and 50°C
pH 6.0, 37°C

substrate: 4-nitrophenyl alpha-L-arabinofuranoside


organism: Acidomyces acidophilus


organism: Acidothermus cellulolyticus

substrate_name: oat spelt xylan

substrate: oat spelt xylan


organism: Acrophialophora nainiana

proof_lines:
insoluble oat spelt xylan, pH 6.5, 55°C
insoluble birchwood xylan, pH 6.5, 55°C
soluble birchwood xylan, pH 6.5, 55°C


organism: Halalkalibacterium halodurans


organism: Paenibacillus curdlanolyticus

substrate_name: soluble birchwood xylan

proof_lines: pH 6.0, temperature not specified in the publication

substrate: soluble birchwood xylan


organism: Penicillium griseofulvum

proof_lines: apparent value for the recombinant enzyme


organism: Pseudozyma hubeiensis

proof_lines:
pH 7.0, 60°C, PhX20, oat spelt xylan
pH 7.0, 60°C, PhX33, oat spelt xylan


substrate_name: beechwood xylan

proof_lines:
Km value is 3.0 mg/ml, pH 5.0, 50°C
Km value is 6.6 mg/ml, pH 5.0, 50°C
Km value is 2.95 mg/ml, pH 5.0, 50°C

substrate: beechwood xylan


organism: Aspergillus terreus

proof_lines: pH and temperature not specified in the publication


organism: Bacillus licheniformis


organism: Duganella sp. PAMC 27433

substrate_name: 4-nitrophenyl beta-D-cellobioside

substrate: 4-nitrophenyl beta-D-cellobioside


organism: Thermoascus aurantiacus


organism: Thermoascus aurantiacus var. levisporus


organism: Thermomonospora sp.


organism: Thermothelomyces heterothallicus


organism: Rasamsonia emersonii

substrate_name: 4-nitrophenyl xylopyranoside

proof_lines:
pH 5, 50°C, xylanase III
pH 5, 50°C, xylanase II

substrate: 4-nitrophenyl xylopyranoside


organism: Remersonia thermophila

substrate_name: soluble oat spelt xylan

substrate: soluble oat spelt xylan


organism: Streptomyces sp. S27


organism: Termitomyces clypeatus

substrate_name: Unknown

substrate: Unknown