column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'A440V + R448S', acceptor column group GLC; column 'Km (mM)' = 0.7; column 'kcat (s^-1)' = 25.0; column 'kcat/Km (mM^-1 s^-1)' = 36. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 25.0 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 25 s^(-1). Km: printed Table 2 cell 0.7 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 0.7 mM. kcat/Km: printed Table 2 cell 36 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 36 s^(-1)*mM^(-1). The exported values are kcat 25.0 s^(-1), Km 0.7 mM and kcat_over_km 35.714285714285715 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'A440V + R448S', acceptor column group GLC: Km = 0.7 mM; kcat = 25.0 s-1; printed kcat/Km = 36 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


enzyme_display_name: alpha,alpha-trehalose phosphorylase

organism: Thermoanaerobacter brockii

substrate_name: D-glucose

source_db: oed

verification_status: corrected

ki_display: Not reported

protein_accession_database: UniProt

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature N657D to reconstruct the assayed variant sequence.

mutation_type: substitution

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657D', acceptor column group GLC; column 'Km (mM)' = 1.8; column 'kcat (s^-1)' = 51.2; column 'kcat/Km (mM^-1 s^-1)' = 28. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 51.2 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 51.2 s^(-1). Km: printed Table 2 cell 1.8 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 1.8 mM. kcat/Km: printed Table 2 cell 28 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 28 s^(-1)*mM^(-1). The exported values are kcat 51.2 s^(-1), Km 1.8 mM and kcat_over_km 28.444444444444446 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657D', acceptor column group GLC: Km = 1.8 mM; kcat = 51.2 s-1; printed kcat/Km = 28 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.

table_label: Table 2: Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes (Biotechnol. Prog., 2012, Vol. 28, No. 5, pages 1257-1262). Printed column heads: Km (mM); kcat (s^-1); kcat/Km (mM^-1 s^-1); each metric head prints one value column under the acceptor group head GLC and one under GAL.

data_origin: experimental

enzyme: alpha,alpha-trehalose phosphorylase

substrate: D-glucose

status: corrected

source: oed


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature N657G to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657G', acceptor column group GLC; column 'Km (mM)' = 2.5; column 'kcat (s^-1)' = 42.7; column 'kcat/Km (mM^-1 s^-1)' = 17. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 42.7 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 42.7 s^(-1). Km: printed Table 2 cell 2.5 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 2.5 mM. kcat/Km: printed Table 2 cell 17 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 17 s^(-1)*mM^(-1). The exported values are kcat 42.7 s^(-1), Km 2.5 mM and kcat_over_km 17.080000000000002 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657G', acceptor column group GLC: Km = 2.5 mM; kcat = 42.7 s-1; printed kcat/Km = 17 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


verification_status: unverified

sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature R448S with inferred residue-numbering offset +24 to reconstruct the assayed variant sequence.

status: unverified


mutation_signature: Unknown

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'WT', acceptor column group GLC; column 'Km (mM)' = 3.2; column 'kcat (s^-1)' = 33.9; column 'kcat/Km (mM^-1 s^-1)' = 11. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 33.9 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 33.9 s^(-1). Km: printed Table 2 cell 3.2 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 3.2 mM. kcat/Km: printed Table 2 cell 11 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 11 s^(-1)*mM^(-1). The exported values are kcat 33.9 s^(-1), Km 3.2 mM and kcat_over_km 10.593749999999998 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'WT', acceptor column group GLC: Km = 3.2 mM; kcat = 33.9 s-1; printed kcat/Km = 11 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


enzyme_display_name: UniProt P14769

organism: Bos taurus

substrate_name: p-nitrophenyl-beta-D-galactoside

source_db: skid

source_license: CC BY-NC-ND 4.0

enzyme: UniProt P14769

substrate: p-nitrophenyl-beta-D-galactoside

source: skid


substrate_name: UDP-N-acetylgalactosamine

km_display: Not reported

kcat_over_km_display: Not reported

substrate: UDP-N-acetylgalactosamine


substrate_name: N-acetyllactosamine

proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=30°C, pH=6

substrate: N-acetyllactosamine


substrate_name: D-galactose

substrate: D-galactose


substrate_name: lactose

proof_lines:
Raw SKiD Env: Temp=37°C, pH=-----
Raw SKiD Env: Temp=30°C, pH=6
Raw SKiD Env: Temp=37°C, pH=7

substrate: lactose


substrate_name: UDP-galactose

kcat_display: Not reported

proof_lines:
Raw SKiD Env: Temp=37°C, pH=-----
Raw SKiD Env: Temp=-----, pH=-----

substrate: UDP-galactose


organism: Homo sapiens

source_db: uniprot

verification_status: verified

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

column_label: Table III columns GalNAc-T13-V1 Vmax and Km; row Muc5AC-3; Km (uM) column = 35+/-6.

raw_value_unit_evidence: Table III row Muc5AC-3, GalNAc-T13-V1 Km = 35+/-6 uM.

row_label: Muc5AC-3; GalNAc-T13-V1

table_label: Table III: Kinetic parameters for peptide glycosylation by GalNAc-T13 splice variants

status: verified

source: uniprot


column_label: Table III columns GalNAc-T13-V1 Vmax and Km; row Muc5AC-13; Km (uM) column = 130+/-15.

raw_value_unit_evidence: Table III row Muc5AC-13, GalNAc-T13-V1 Km = 130+/-15 uM.

row_label: Muc5AC-13; GalNAc-T13-V1


column_label: Table II columns GalNAc-T16 Vmax (pmol/h) and Km (uM); row Muc5AC-3.

normalized_value_unit_evidence: Actual primary Km is 0.299 mM; 310 is Vmax, not Km.

raw_value_unit_evidence: Table II row Muc5AC-3, GalNAc-T16 Vmax = 310+/-25 pmol/h and Km = 299+/-30 uM.

row_label: Muc5AC-3; GalNAc-T16

table_label: Table II: Kinetic parameters for peptide glycosylation by GalNAc-T1 and -T16


column_label: Table II columns GalNAc-T16 Vmax (pmol/h) and Km (uM); row Muc5AC-13.

normalized_value_unit_evidence: Actual primary Km is 0.115 mM; 390 is Vmax, not Km.

raw_value_unit_evidence: Table II row Muc5AC-13, GalNAc-T16 Vmax = 390+/-32 pmol/h and Km = 115+/-10 uM.

row_label: Muc5AC-13; GalNAc-T16


column_label: Table II columns GalNAc-T16 Vmax (pmol/h) and Km (uM); row EA2.

normalized_value_unit_evidence: Actual primary Km is 0.172 mM; 332 is Vmax, not Km.

raw_value_unit_evidence: Table II row EA2, GalNAc-T16 Vmax = 332+/-37 pmol/h and Km = 172+/-18 uM.

row_label: EA2; GalNAc-T16


organism: Mus musculus

substrate_name: ganglioside GA1

substrate: ganglioside GA1


substrate_name: ganglioside GM1a

substrate: ganglioside GM1a


proof_lines:
GA1 (Gg4Cer)
FGA1 (fucosyl GA1)
GM1 (II3NeuAc-Gg4Cer)


kcat_over_km_origin: calculated

sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature L310A with inferred residue-numbering offset +11 to reconstruct the assayed variant sequence.


substrate_name: UDP-N-acetyl-alpha-D-galactosamine

substrate: UDP-N-acetyl-alpha-D-galactosamine


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature N657L to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657L', acceptor column group GAL; column 'Km (mM)' = 61.1; column 'kcat (s^-1)' = 33.3; column 'kcat/Km (mM^-1 s^-1)' = 0.545. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 33.3 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 33.3 s^(-1). Km: printed Table 2 cell 61.1 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 61.1 mM. kcat/Km: printed Table 2 cell 0.545 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.545 s^(-1)*mM^(-1). The exported values are kcat 33.3 s^(-1), Km 61.1 mM and kcat_over_km 0.5450081833060556 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657L', acceptor column group GAL: Km = 61.1 mM; kcat = 33.3 s-1; printed kcat/Km = 0.545 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature N657R to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657R', acceptor column group GAL; column 'Km (mM)' = 62.5; column 'kcat (s^-1)' = 38.3; column 'kcat/Km (mM^-1 s^-1)' = 0.613. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 38.3 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 38.3 s^(-1). Km: printed Table 2 cell 62.5 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 62.5 mM. kcat/Km: printed Table 2 cell 0.613 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.613 s^(-1)*mM^(-1). The exported values are kcat 38.3 s^(-1), Km 62.5 mM and kcat_over_km 0.6127999999999999 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657R', acceptor column group GAL: Km = 62.5 mM; kcat = 38.3 s-1; printed kcat/Km = 0.613 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature N657Y to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657Y', acceptor column group GAL; column 'Km (mM)' = 45.8; column 'kcat (s^-1)' = 23.3; column 'kcat/Km (mM^-1 s^-1)' = 0.509. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 23.3 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 23.3 s^(-1). Km: printed Table 2 cell 45.8 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 45.8 mM. kcat/Km: printed Table 2 cell 0.509 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.509 s^(-1)*mM^(-1). The exported values are kcat 23.3 s^(-1), Km 45.8 mM and kcat_over_km 0.5087336244541485 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657Y', acceptor column group GAL: Km = 45.8 mM; kcat = 23.3 s-1; printed kcat/Km = 0.509 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature P588H to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'P588H', acceptor column group GAL; column 'Km (mM)' = 96.0; column 'kcat (s^-1)' = 54.3; column 'kcat/Km (mM^-1 s^-1)' = 0.566. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 54.3 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 54.3 s^(-1). Km: printed Table 2 cell 96.0 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 96 mM. kcat/Km: printed Table 2 cell 0.566 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.566 s^(-1)*mM^(-1). The exported values are kcat 54.3 s^(-1), Km 96.0 mM and kcat_over_km 0.5656249999999999 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'P588H', acceptor column group GAL: Km = 96.0 mM; kcat = 54.3 s-1; printed kcat/Km = 0.566 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature R448D to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448D', acceptor column group GAL; column 'Km (mM)' = 47.5; column 'kcat (s^-1)' = 21.1; column 'kcat/Km (mM^-1 s^-1)' = 0.444. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 21.1 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 21.1 s^(-1). Km: printed Table 2 cell 47.5 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 47.5 mM. kcat/Km: printed Table 2 cell 0.444 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.444 s^(-1)*mM^(-1). The exported values are kcat 21.1 s^(-1), Km 47.5 mM and kcat_over_km 0.4442105263157895 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448D', acceptor column group GAL: Km = 47.5 mM; kcat = 21.1 s-1; printed kcat/Km = 0.444 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature R448F with inferred residue-numbering offset +24 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature R448N to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448N', acceptor column group GAL; column 'Km (mM)' = 53.6; column 'kcat (s^-1)' = 32.4; column 'kcat/Km (mM^-1 s^-1)' = 0.604. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 32.4 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 32.4 s^(-1). Km: printed Table 2 cell 53.6 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 53.6 mM. kcat/Km: printed Table 2 cell 0.604 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.604 s^(-1)*mM^(-1). The exported values are kcat 32.4 s^(-1), Km 53.6 mM and kcat_over_km 0.6044776119402985 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448N', acceptor column group GAL: Km = 53.6 mM; kcat = 32.4 s-1; printed kcat/Km = 0.604 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature R448S to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448S', acceptor column group GAL; column 'Km (mM)' = 33.5; column 'kcat (s^-1)' = 31.9; column 'kcat/Km (mM^-1 s^-1)' = 0.952. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 31.9 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 31.9 s^(-1). Km: printed Table 2 cell 33.5 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 33.5 mM. kcat/Km: printed Table 2 cell 0.952 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.952 s^(-1)*mM^(-1). The exported values are kcat 31.9 s^(-1), Km 3.25 mM and kcat_over_km 9.815384615384614 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448S', acceptor column group GAL: Km = 33.5 mM; kcat = 31.9 s-1; printed kcat/Km = 0.952 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature R448S/N657Y to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448S + N657Y', acceptor column group GAL; column 'Km (mM)' = 38.0; column 'kcat (s^-1)' = 14.0; column 'kcat/Km (mM^-1 s^-1)' = 0.368. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 14.0 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 14 s^(-1). Km: printed Table 2 cell 38.0 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 38 mM. kcat/Km: printed Table 2 cell 0.368 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.368 s^(-1)*mM^(-1). The exported values are kcat 14.0 s^(-1), Km 38.0 mM and kcat_over_km 0.3684210526315789 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448S + N657Y', acceptor column group GAL: Km = 38.0 mM; kcat = 14.0 s-1; printed kcat/Km = 0.368 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature R448V to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448V', acceptor column group GAL; column 'Km (mM)' = 43.7; column 'kcat (s^-1)' = 26.2; column 'kcat/Km (mM^-1 s^-1)' = 0.600. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 26.2 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 26.2 s^(-1). Km: printed Table 2 cell 43.7 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 43.7 mM. kcat/Km: printed Table 2 cell 0.600 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 0.6 s^(-1)*mM^(-1). The exported values are kcat 26.2 s^(-1), Km 43.7 mM and kcat_over_km 0.5995423340961098 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448V', acceptor column group GAL: Km = 43.7 mM; kcat = 26.2 s-1; printed kcat/Km = 0.600 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature N657I to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657I', acceptor column group GLC; column 'Km (mM)' = 2.1; column 'kcat (s^-1)' = 46.9; column 'kcat/Km (mM^-1 s^-1)' = 22. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 46.9 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 46.9 s^(-1). Km: printed Table 2 cell 2.1 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 2.1 mM. kcat/Km: printed Table 2 cell 22 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 22 s^(-1)*mM^(-1). The exported values are kcat 46.9 s^(-1), Km 2.1 mM and kcat_over_km 22.333333333333332 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657I', acceptor column group GLC: Km = 2.1 mM; kcat = 46.9 s-1; printed kcat/Km = 22 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657L', acceptor column group GLC; column 'Km (mM)' = 2.2; column 'kcat (s^-1)' = 43.3; column 'kcat/Km (mM^-1 s^-1)' = 20. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 43.3 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 43.3 s^(-1). Km: printed Table 2 cell 2.2 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 2.2 mM. kcat/Km: printed Table 2 cell 20 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 20 s^(-1)*mM^(-1). The exported values are kcat 43.3 s^(-1), Km 2.2 mM and kcat_over_km 19.68181818181818 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657L', acceptor column group GLC: Km = 2.2 mM; kcat = 43.3 s-1; printed kcat/Km = 20 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657R', acceptor column group GLC; column 'Km (mM)' = 1.3; column 'kcat (s^-1)' = 31.4; column 'kcat/Km (mM^-1 s^-1)' = 24. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 31.4 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 31.4 s^(-1). Km: printed Table 2 cell 1.3 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 1.3 mM. kcat/Km: printed Table 2 cell 24 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 24 s^(-1)*mM^(-1). The exported values are kcat 31.4 s^(-1), Km 1.3 mM and kcat_over_km 24.153846153846153 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657R', acceptor column group GLC: Km = 1.3 mM; kcat = 31.4 s-1; printed kcat/Km = 24 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'N657Y', acceptor column group GLC; column 'Km (mM)' = 3.0; column 'kcat (s^-1)' = 53.9; column 'kcat/Km (mM^-1 s^-1)' = 18. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 53.9 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 53.9 s^(-1). Km: printed Table 2 cell 3.0 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 3 mM. kcat/Km: printed Table 2 cell 18 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 18 s^(-1)*mM^(-1). The exported values are kcat 53.9 s^(-1), Km 3.0 mM and kcat_over_km 17.966666666666665 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'N657Y', acceptor column group GLC: Km = 3.0 mM; kcat = 53.9 s-1; printed kcat/Km = 18 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature P588H with inferred residue-numbering offset +5 to reconstruct the assayed variant sequence.

proof_lines:
Thermoanaerobacter brockii
trehalose phosphorylase
R448S


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448D', acceptor column group GLC; column 'Km (mM)' = 1.2; column 'kcat (s^-1)' = 18.6; column 'kcat/Km (mM^-1 s^-1)' = 16. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 18.6 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 18.6 s^(-1). Km: printed Table 2 cell 1.2 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 1.2 mM. kcat/Km: printed Table 2 cell 16 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 16 s^(-1)*mM^(-1). The exported values are kcat 18.6 s^(-1), Km 1.2 mM and kcat_over_km 15.500000000000002 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448D', acceptor column group GLC: Km = 1.2 mM; kcat = 18.6 s-1; printed kcat/Km = 16 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q8L164 and applied mutation signature R448F to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448F', acceptor column group GLC; column 'Km (mM)' = 1.2; column 'kcat (s^-1)' = 21.7; column 'kcat/Km (mM^-1 s^-1)' = 18. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 21.7 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 21.7 s^(-1). Km: printed Table 2 cell 1.2 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 1.2 mM. kcat/Km: printed Table 2 cell 18 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 18 s^(-1)*mM^(-1). The exported values are kcat 21.7 s^(-1), Km 1.2 mM and kcat_over_km 18.083333333333332 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448F', acceptor column group GLC: Km = 1.2 mM; kcat = 21.7 s-1; printed kcat/Km = 18 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448N', acceptor column group GLC; column 'Km (mM)' = 1.5; column 'kcat (s^-1)' = 30.6; column 'kcat/Km (mM^-1 s^-1)' = 20. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 30.6 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 30.6 s^(-1). Km: printed Table 2 cell 1.5 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 1.5 mM. kcat/Km: printed Table 2 cell 20 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 20 s^(-1)*mM^(-1). The exported values are kcat 30.6 s^(-1), Km 1.5 mM and kcat_over_km 20.400000000000002 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448N', acceptor column group GLC: Km = 1.5 mM; kcat = 30.6 s-1; printed kcat/Km = 20 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448S', acceptor column group GLC; column 'Km (mM)' = 1.5; column 'kcat (s^-1)' = 26.3; column 'kcat/Km (mM^-1 s^-1)' = 18. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 26.3 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 26.3 s^(-1). Km: printed Table 2 cell 1.5 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 1.5 mM. kcat/Km: printed Table 2 cell 18 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 18 s^(-1)*mM^(-1). The exported values are kcat 26.3 s^(-1), Km 1.5 mM and kcat_over_km 17.533333333333335 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448S', acceptor column group GLC: Km = 1.5 mM; kcat = 26.3 s-1; printed kcat/Km = 18 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


column_label: Table 2 (Kinetic Parameters for Glucose and Galactose of Wild-Type Trehalose Phosphorylase and Variant Enzymes), printed body row 'R448S + N657Y', acceptor column group GLC; column 'Km (mM)' = 0.6; column 'kcat (s^-1)' = 7.0; column 'kcat/Km (mM^-1 s^-1)' = 12. The number printed beside each of these cells is that cell's own printed uncertainty and is not an exported metric.

normalized_value_unit_evidence: kcat: printed Table 2 cell 7.0 in a column headed kcat (s^-1); s^-1 is already the canonical s^(-1), so no unit conversion applies and the value is 7 s^(-1). Km: printed Table 2 cell 0.6 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 0.6 mM. kcat/Km: printed Table 2 cell 12 in a column headed kcat/Km (mM^-1 s^-1); 1 mM^-1 s^-1 is 1 s^-1 mM^-1, so no unit conversion applies and the value is 12 s^(-1)*mM^(-1). The exported values are kcat 7.0 s^(-1), Km 0.6 mM and kcat_over_km 11.666666666666668 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, printed body row 'R448S + N657Y', acceptor column group GLC: Km = 0.6 mM; kcat = 7.0 s-1; printed kcat/Km = 12 in a column headed kcat/Km (mM^-1 s^-1). The number printed beside each of these three cells is that cell's own printed uncertainty and is not a kinetic metric the export schema carries.


substrate_name: galacto-N-biose

assay_conditions_summary: pH 6.1; cofactor ATP

column_label: Table II | row Se (fut2) | column Galacto-N-biose Km (uM) | 0.38

raw_value_unit_evidence: Table II, Se (fut2), Galacto-N-biose, Km (uM) = 0.38

row_label: Se (fut2)

table_label: Table II. Kinetic parameters of bovine recombinant alpha2-fucosyltransferases

substrate: galacto-N-biose


substrate_name: Galacto-N-biose

column_label: Table II | row H (fut1) | column Galacto-N-biose Km (uM) | 0.26

raw_value_unit_evidence: Table II, H (fut1), Galacto-N-biose, Km (uM) = 0.26

row_label: H (fut1)

substrate: Galacto-N-biose


column_label: Table II | row H (fut1) | column GM1 Km (uM) | 1.29

raw_value_unit_evidence: Table II, H (fut1), GM1, Km (uM) = 1.29


enzyme_display_name: flavonol 3-O-glucosyltransferase

organism: Marchantia emarginata

substrate_name: apigenin

source_db: brenda

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

enzyme: flavonol 3-O-glucosyltransferase

substrate: apigenin

source: brenda


substrate_name: isorhamnetin

substrate: isorhamnetin


substrate_name: quercetin

substrate: quercetin


substrate_name: myricetin

substrate: myricetin


substrate_name: kaempferol

substrate: kaempferol


substrate_name: luteolin

substrate: luteolin


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: GA1 MFUT-I MFUT-II


substrate_name: lacto-N-biose

assay_conditions_summary: pH 6

column_label: Figure 3C reports apparent Km = 3.6 mM for lacto-N-biose I; Table I lists pcDNAI-alpha(1,2)FTSe lacto-N-biose I = 3.6 mM, This work.

raw_value_unit_evidence: Km raw_value=3.6 raw_unit=mM

row_label: Sec2-encoded alpha(1,2)fucosyltransferase; lacto-N-biose I

table_label: Figure 3 caption and Table I

substrate: lacto-N-biose


column_label: Figure 3D reports apparent Km = 3.8 mM for N-acetyllactosamine; Table I lists pcDNAI-alpha(1,2)FTSe N-acetyllactosamine = 3.8 mM, This work.

raw_value_unit_evidence: Km raw_value=3.8 raw_unit=mM


substrate_name: GDP-fucose

column_label: Figure 3E reports apparent Km = 197 microM for GDP-fucose; Table I lists pcDNAI-alpha(1,2)FTSe GDP-fucose = 0.197 mM, This work.

raw_value_unit_evidence: Km raw_value=197 raw_unit=microM

substrate: GDP-fucose


organism: Glycine max


substrate_name: fisetin

substrate: fisetin


substrate_name: UDPglucose

substrate: UDPglucose


organism: Raphanus sativus

substrate_name: 2,5-dihydroxy benzoate

proof_lines: pH 7.0, 30°C, recombinant wild-type enzyme

substrate: 2,5-dihydroxy benzoate


substrate_name: 2,4-dihydroxy benzoate

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

substrate: 2,4-dihydroxy benzoate


substrate_name: UDP-glucose

substrate: UDP-glucose


organism: Camellia sinensis


organism: Brassica oleracea


organism: Hippeastrum sp.


substrate_name: cyanidin

substrate: cyanidin


organism: Triticum aestivum


proof_lines:
reaction with 2,4,5-trichlorophenol
reaction with quercetin


organism: Arabidopsis thaliana

substrate_name: trans-Resveratrol; UDP-D-glucose

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

substrate: trans-Resveratrol; UDP-D-glucose


substrate_name: UDP-D-glucose; trans-Resveratrol

substrate: UDP-D-glucose; trans-Resveratrol


organism: Stevia rebaudiana


organism: Cavia porcellus

substrate_name: UDP-N-acetylgalactosamine

substrate: UDP-N-acetylgalactosamine


substrate_name: globoside

substrate: globoside


enzyme_display_name: N-acetyllactosamine synthase

organism: Vigna radiata

enzyme: N-acetyllactosamine synthase


substrate_name: UDP-galactose

substrate: UDP-galactose


organism: Petroselinum crispum

substrate_name: quercetin

substrate: quercetin


organism: Prunus yedoensis


organism: Vigna mungo


substrate_name: UDP-D-glucose

substrate: UDP-D-glucose


organism: Vitis labrusca


substrate_name: UDP-alpha-D-glucose

proof_lines: using cyanidin as cosubstrate, in 100 mM Tris-HCl pH 8.0, at 30°C

substrate: UDP-alpha-D-glucose


organism: Paederia scandens

substrate_name: kaempferol

substrate: kaempferol


organism: Citrus x paradisi

assay_conditions_summary: pH 7.5; 303.15 K; buffer 50 mM phosphate

proof_lines: apparent value, in 50 mM phosphate buffer (pH 7.5), at 40°C


proof_lines: recombinant enzyme, in 50 mM HEPES, at pH 8.0 and 30°C


organism: Trichoplusia ni

substrate_name: p-nitrophenyl-GlcNAc

substrate: p-nitrophenyl-GlcNAc


assay_conditions_summary: pH 7.5; buffer 50 mM phosphate


substrate_name: myricetin

substrate: myricetin


organism: Bos taurus

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature E317Q with inferred residue-numbering offset -6 to reconstruct the assayed variant sequence.

proof_lines: 37°C, mutant enzyme E317Q, hydrolase reaction

proof_lines: 37°C, mutant enzyme E317Q, galactosyltransferase reaction


substrate_name: lactose

substrate: lactose


organism: Canis lupus familiaris


substrate_name: N-Acetylglucosamine; UDPgalactose

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

source_databases_merged: sabio_rk skid uniprot

substrate: N-Acetylglucosamine; UDPgalactose


enzyme_display_name: Beta-1,4-galactosyltransferase 4

organism: Homo sapiens

substrate_name: GlcNAc-B-S-pNP

enzyme: Beta-1,4-galactosyltransferase 4

substrate: GlcNAc-B-S-pNP


enzyme_display_name: Beta-1,4-galactosyltransferase 3

enzyme: Beta-1,4-galactosyltransferase 3


enzyme_display_name: Beta-1,4-galactosyltransferase 2

enzyme: Beta-1,4-galactosyltransferase 2


organism: Rattus norvegicus

substrate_name: N-Acetyl-D-glucosamine; UDP-D-galactose

sequence: PGATLQXAXXLLVAVXAXPPPPLGVXPKPXPG

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

substrate: N-Acetyl-D-glucosamine; UDP-D-galactose


substrate_name: ganglioside GD3

proof_lines: inhibition of GM2 synthesis, pH 7.35, 37°C

substrate: ganglioside GD3


sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)


organism: Vitis vinifera

substrate_name: dTDP-Glc (in the presence of 100 uM quercitin)


organism: Mus musculus


enzyme_display_name: Anthocyanidin 3-O-glucosyltransferase 1

organism: Fragaria ananassa

substrate_name: pelargonidin

proof_lines: The kinetic constants are determined for the recombinant His(6)-tagged protein.

enzyme: Anthocyanidin 3-O-glucosyltransferase 1

substrate: pelargonidin


substrate_name: UDP-glucose

substrate: UDP-glucose


sequence_source: multiple

source_databases_merged: brenda uniprot


substrate_name: delphinidin

substrate: delphinidin


substrate_name: cyanidin

substrate: cyanidin


substrate_name: malvidin

substrate: malvidin


substrate_name: UDPglucose

substrate: UDPglucose


substrate_name: Unknown

substrate: Unknown


enzyme_display_name: UDP-glucose flavonoid 3-O-glucosyltransferase 7

proof_lines: The kinetic constants are determined for the recombinant GST-fusion protein.


enzyme_display_name: UDP-glucose flavonoid 3-O-glucosyltransferase 6


ki_unit: mM

proof_lines: inhibition of GD2 synthesis, pH 7.35, 37°C


substrate_name: UDP-galactose

substrate: UDP-galactose


substrate_name: D-GlcNAc

substrate: D-GlcNAc


substrate_name: UDP-N-acetyl-D-galactosamine

proof_lines:
cosubstrate GM3
cosubstrate SM3

substrate: UDP-N-acetyl-D-galactosamine


substrate_name: kaempferol

substrate: kaempferol


substrate_name: UDP-GlcNAc (in the presence of 100 uM quercitin)

proof_lines: VvGT1 UDP-glucose kaempferol


substrate_name: UDP-Man (in the presence of 100 uM quercitin)


substrate_name: GDP-Glc (in the presence of 100 uM quercitin)


substrate_name: UDP-Xyl (in the presence of 100 uM quercitin)


organism: Camellia sinensis

column_label: Table 1; row Kaempferol under enzyme CsUGT78A14, GlcT activity; column Km (uM) raw 30.9+/-3.5; column kcat (S^-1) raw 6.75+/-0.03; column kcat/Km raw 0.219 s^-1 uM^-1.

normalized_value_unit_evidence: Km 30.9 uM = 30.9/1000 = 0.0309 mM. kcat 6.75 s^-1 = 6.75 s^-1 (already canonical). kcat/Km taken by division of this same row's kcat and Km: 6.75 / 0.0309 = 218.45 s^-1 mM^-1; the table's own rounded efficiency 0.219 s^-1 uM^-1 = 0.219 x 1000 = 219 s^-1 mM^-1, consistent with the division at the rounding printed in the table.

raw_value_unit_evidence: Table 1, CsUGT78A14 GlcT activity block, Kaempferol row: Km 30.9+/-3.5 uM; kcat 6.75+/-0.03 s^-1; kcat/Km 0.219 s^-1 uM^-1.

row_label: CsUGT78A14 GlcT activity; Kaempferol

table_label: Table 1. Kinetic parameters of recombinant CsUGT78A14 and CsUGT78A15 proteins.


substrate_name: myricetin

column_label: Table 1; row Myricetin under enzyme CsUGT78A14, GlcT activity; column Km (uM) raw 23.8+/-2.1; column kcat (S^-1) raw 3.11+/-0.04; column kcat/Km raw 0.131 s^-1 uM^-1.

normalized_value_unit_evidence: Km 23.8 uM = 23.8/1000 = 0.0238 mM. kcat 3.11 s^-1 = 3.11 s^-1 (already canonical). kcat/Km taken by division of this same row's kcat and Km: 3.11 / 0.0238 = 130.67 s^-1 mM^-1; the table's own rounded efficiency 0.131 s^-1 uM^-1 = 0.131 x 1000 = 131 s^-1 mM^-1, consistent with the division at the rounding printed in the table.

raw_value_unit_evidence: Table 1, CsUGT78A14 GlcT activity block, Myricetin row: Km 23.8+/-2.1 uM; kcat 3.11+/-0.04 s^-1; kcat/Km 0.131 s^-1 uM^-1.

row_label: CsUGT78A14 GlcT activity; Myricetin

substrate: myricetin


substrate_name: quercetin

column_label: Table 1; row Quercetin under enzyme CsUGT78A14, GlcT activity; column Km (uM) raw 11.9+/-1.6; column kcat (S^-1) raw 3.32+/-0.07; column kcat/Km raw 0.280 s^-1 uM^-1.

normalized_value_unit_evidence: Km 11.9 uM = 11.9/1000 = 0.0119 mM. kcat 3.32 s^-1 = 3.32 s^-1 (already canonical). kcat/Km taken by division of this same row's kcat and Km: 3.32 / 0.0119 = 278.99 s^-1 mM^-1; the table's own rounded efficiency 0.280 s^-1 uM^-1 = 0.280 x 1000 = 280 s^-1 mM^-1, consistent with the division at the rounding printed in the table.

raw_value_unit_evidence: Table 1, CsUGT78A14 GlcT activity block, Quercetin row: Km 11.9+/-1.6 uM; kcat 3.32+/-0.07 s^-1; kcat/Km 0.280 s^-1 uM^-1.

row_label: CsUGT78A14 GlcT activity; Quercetin

substrate: quercetin


column_label: Table 1; row UDP-Glc* under enzyme CsUGT78A14, GlcT activity; column Km (uM) raw 14.1+/-1.8; column kcat (S^-1) raw 3.61+/-0.02; column kcat/Km raw 0.257 s^-1 uM^-1.

normalized_value_unit_evidence: Km 14.1 uM = 14.1/1000 = 0.0141 mM. kcat 3.61 s^-1 = 3.61 s^-1 (already canonical). kcat/Km taken by division of this same row's kcat and Km: 3.61 / 0.0141 = 256.03 s^-1 mM^-1; the table's own rounded efficiency 0.257 s^-1 uM^-1 = 0.257 x 1000 = 257 s^-1 mM^-1, consistent with the division at the rounding printed in the table.

raw_value_unit_evidence: Table 1, CsUGT78A14 GlcT activity block, UDP-Glc* row: Km 14.1+/-1.8 uM; kcat 3.61+/-0.02 s^-1; kcat/Km 0.257 s^-1 uM^-1.

row_label: CsUGT78A14 GlcT activity; UDP-Glc*


organism: Arabidopsis thaliana

substrate_name: curcumin

column_label: Table 3 section Curcumin, row UGT71C1, column Km (mM), gives 0.15 +/- 0.01

row_label: UGT71C1 / Curcumin

table_label: Table 3

substrate: curcumin


substrate_name: trans-resveratrol

column_label: Table 3 section Trans-resveratrol (Trans-resveratrol-3-O-glucoside formation), row UGT71C1, column Km (mM), gives a0.5 +/- 0.1

row_label: UGT71C1 / Trans-resveratrol 3-O-glucoside formation

substrate: trans-resveratrol


substrate_name: vanillin

column_label: Table 3 section Vanillin, row UGT71C1, column Km (mM), gives 1.12 +/- 0.04

row_label: UGT71C1 / Vanillin

substrate: vanillin


substrate_name: etoposide

column_label: Table 3 section Etoposide, row UGT71C1, column Km (mM), gives 2.7 +/- 0.2

row_label: UGT71C1 / Etoposide

substrate: etoposide


organism: Fragaria x ananassa

substrate_name: isorhamnetin

substrate: isorhamnetin


substrate_name: UDP-alpha-D-glucose

proof_lines:
pH 7.0, 30°C, enzyme FaGT7
pH 7.0, 30°C, enzyme FaGT6

substrate: UDP-alpha-D-glucose


substrate_name: ganglioside GM3

substrate: ganglioside GM3


smiles: [Gd]


substrate_name: lactosylceramide

substrate: lactosylceramide


enzyme_display_name: sucrose:sucrose fructosyltransferase

organism: Agave tequilana

substrate_name: sucrose

enzyme: sucrose:sucrose fructosyltransferase

substrate: sucrose


organism: Helianthus tuberosus


organism: Cichorium intybus


substrate_name: fructose

substrate: fructose


organism: Vitis labrusca

substrate_name: cyanidin

column_label: Official ScienceDirect HTML Table 1 row Cyanidin (3), VL3GT columns KM=4.79 +/- 0.69 uM, kcat=7.02 in 10^-4 s^-1 units, kcat/KM=146 M^-1 s^-1; Section 5.7 states acceptor kinetics used 9 mM UDPG with varying cyanidin.

row_label: Cyanidin (3) / VL3GT

table_label: Table 1. Kinetic analysis of VL3GT and VV3GT with cyanidin, quercetin and UDPG.

substrate: cyanidin


column_label: Official ScienceDirect HTML Table 1 row Quercetin (6), VL3GT columns KM=2.16 +/- 0.28 uM, kcat=1.13 in 10^-4 s^-1 units, kcat/KM=52 M^-1 s^-1; Section 5.7 states acceptor kinetics used 9 mM UDPG with varying quercetin.

row_label: Quercetin (6) / VL3GT


column_label: Official ScienceDirect HTML Table 1 row UDPG (cyanidin)/(quercetin), VL3GT columns KM=914 +/- 0.38 uM, kcat=2.47 in 10^-4 s^-1 units, kcat/KM=0.27 M^-1 s^-1; Section 5.7 states UDPG kinetics used 6 uM cyanidin.

row_label: UDPG (cyanidin) / VL3GT


substrate_name: kaempferol

column_label: Publisher PDF page 6, Table 2, Kae (5) row under Km(app), kcat, and kcat/Km(app).

normalized_value_unit_evidence: Km=0.012 mM; kcat=0.5955 s^-1; explicit paper efficiency=49.6 s^-1 mM^-1.

row_label: Kae (5)

table_label: Table 2, Kinetic parameters of recombinant flavonol 3-O-GT


column_label: Publisher PDF page 6, Table 2, Myr (7) row under Km(app), kcat, and kcat/Km(app).

normalized_value_unit_evidence: Km=0.033 mM; kcat=0.6252 s^-1; explicit paper efficiency=18.9 s^-1 mM^-1.

row_label: Myr (7)


column_label: Publisher PDF page 6, Table 2, Que (6) row under Km(app), kcat, and kcat/Km(app).

normalized_value_unit_evidence: Km=0.067 mM; kcat=1.0470 s^-1; explicit paper efficiency=15.6 s^-1 mM^-1.

row_label: Que (6)


substrate_name: UDP-glucose

column_label: Publisher PDF page 6, Table 2, UDPG row and footnote b under Km(app), kcat, and kcat/Km(app).

normalized_value_unit_evidence: Km=0.669 mM; kcat=1.4425 s^-1; explicit paper efficiency=2.2 s^-1 mM^-1.

raw_value_unit_evidence: Km(app)=669 microM (footnote b: quercetin co-substrate); kcat=1.4425 s^-1; kcat/Km(app)=0.0022 microM^-1 s^-1.

row_label: UDPG

substrate: UDP-glucose


enzyme_display_name: Alpha,alpha-trehalose phosphorylase

organism: Geobacillus stearothermophilus

substrate_name: trehalose

column_label: Table 3 lists Apparent Km (mM) for phosphorolysis with Trehalose under the B. stearo(a) column as 4.1; the Kinetics prose also states Km for trehalose and Pi were 4.1 and 1.1 mM respectively.

raw_value_unit_evidence: Km raw_value=4.1 raw_unit=mM

row_label: Apparent Km (mM) / Phosphorolysis / Trehalose / B. stearo(a) column

table_label: Table 3. Properties of Trehalose Phosphorylases from Various Origins

enzyme: Alpha,alpha-trehalose phosphorylase

substrate: trehalose


substrate_name: phosphate

column_label: Table 3 lists Apparent Km (mM) for phosphorolysis with Phosphate under the B. stearo(a) column as 1.1; the Kinetics prose also states Km for trehalose and Pi were 4.1 and 1.1 mM respectively.

raw_value_unit_evidence: Km raw_value=1.1 raw_unit=mM

row_label: Apparent Km (mM) / Phosphorolysis / Phosphate / B. stearo(a) column

substrate: phosphate


enzyme_display_name: type 1 galactoside alpha-(1,2)-fucosyltransferase

organism: Dictyostelium discoideum

substrate_name: GDP-L-fucose

column_label: Abstract states apparent calculated Km values for GDP-Fuc and lacto-N-biose I are 1.27 microM and 2.80 mM, respectively.

raw_value_unit_evidence: Km raw_value=1.27 raw_unit=microM

row_label: Dictyostelium discoideum cytosolic fucosyltransferase with GDP-Fuc

table_label: PubMed abstract PMID 8849691

substrate: GDP-L-fucose


substrate_name: lacto-N-biose I

enzyme: type 1 galactoside alpha-(1,2)-fucosyltransferase

substrate: lacto-N-biose I


substrate_name: N-acetyllactosamine

substrate: N-acetyllactosamine


substrate_name: lacto-N-biose

substrate: lacto-N-biose


substrate_name: GDP-fucose

substrate: GDP-fucose


substrate_name: UDP-N-acetyl-D-galactosamine

proof_lines: pH 7.3, 37°C, membrane-bound complete enzyme

substrate: UDP-N-acetyl-D-galactosamine


organism: Escherichia coli

substrate_name: D-lactose

substrate: D-lactose


substrate_name: GDP-beta-L-fucose

substrate: GDP-beta-L-fucose


enzyme: UDP-glucose flavonoid 3-O-glucosyltransferase 6


substrate_name: isorhamnetin

enzyme: UDP-glucose flavonoid 3-O-glucosyltransferase 7

substrate: isorhamnetin


organism: Drosophila melanogaster

substrate_name: UDP-GalNAc

substrate: UDP-GalNAc


organism: Solanum lycopersicum

substrate_name: p-coumaric acid

substrate: p-coumaric acid


substrate_name: ferulic acid

substrate: ferulic acid


substrate_name: caffeic acid

substrate: caffeic acid


substrate_name: Sinapic acid

substrate: Sinapic acid


substrate_name: UDPglucose

substrate: UDPglucose


organism: Lathyrus oleraceus

substrate_name: UDP-glucose

substrate: UDP-glucose


substrate_name: 1-naphthalene acetate

substrate: 1-naphthalene acetate


enzyme_display_name: 1,3-beta-D-glucan phosphorylase

organism: Unknown

substrate_name: laminaribiose

enzyme: 1,3-beta-D-glucan phosphorylase

substrate: laminaribiose


substrate_name: alpha-D-glucose

substrate: alpha-D-glucose


substrate_name: Laminarihexaose

substrate: Laminarihexaose


substrate_name: laminaritriose

substrate: laminaritriose


substrate_name: laminaritetraose

substrate: laminaritetraose


substrate_name: Laminaripentaose

substrate: Laminaripentaose


substrate_name: Unknown

source_db: unknown

source_license: see source

substrate: Unknown

source: unknown


enzyme_display_name: UniProt P08037

organism: Bos taurus

substrate_name: UDP-galactose

source_license: CC BY 4.0; CC BY-NC-ND 4.0

source_databases_merged: skid uniprot

enzyme: UniProt P08037

substrate: UDP-galactose


enzyme_display_name: Beta-1,4-galactosyltransferase 1

substrate_name: D-GlcNAc

enzyme: Beta-1,4-galactosyltransferase 1

substrate: D-GlcNAc


organism: Streptococcus mutans

substrate_name: sucrose

substrate: sucrose


substrate_name: Sucrose; Dextran

substrate: Sucrose; Dextran


organism: Biomphalaria glabrata

substrate_name: UDP-N-acetyl-alpha-D-galactosamine (UDP-GalNAc) (at pH 7 and 37 degrees Celsius)

assay_conditions_summary: pH 7


enzyme_display_name: sinapate 1-glucosyltransferase

organism: Daucus carota subsp. sativus

substrate_name: sinapate

enzyme: sinapate 1-glucosyltransferase

substrate: sinapate


organism: Daucus carota


substrate_name: UDP-Gal

proof_lines: kcat is 3.14 min(-1) with UDP-Gal as substrate. kcat is 3.53 min(-1) with APDTRP glycopeptide as substrate. kcat is 1.91 min(-1) with APDSRP glycopeptide as substrate. kcat is 1.62 min(-1) with YAATGPF glycopeptide as substrate. kcat is 3.6

substrate: UDP-Gal


organism: Arabidopsis thaliana

substrate_name: UDPglucose; Sinapate

substrate: UDPglucose; Sinapate


substrate_name: Sinapate; UDPglucose

substrate: Sinapate; UDPglucose


substrate_name: UDPglucose; Indole-3-acetate

substrate: UDPglucose; Indole-3-acetate


organism: Rattus norvegicus

substrate_name: UDPgalactose; GalNAc-alpha1-O-phenyl

substrate: UDPgalactose; GalNAc-alpha1-O-phenyl


substrate_name: Cinnamic acid; UDPglucose

substrate: Cinnamic acid; UDPglucose


substrate_name: UDPglucose; Ferulate

substrate: UDPglucose; Ferulate


organism: Punica granatum

proof_lines: kcat is 0.52 sec(-1) with gallic acid as substrate. kcat is 0.61 sec(-1) with 4-hydroxybenzoic acid as substrate. kcat is 0.76 sec(-1) with 3,4-dihydroxybenzoic acid as substrate. kcat is 0.64 sec(-1) with caffeic acid as substrate. kcat is


proof_lines: kcat is 0.55 sec(-1) with gallic acid as substrate. kcat is 0.6 sec(-1) with 4-hydroxybenzoic acid as substrate. kcat is 1.35 sec(-1) with 3,4-dihydroxybenzoic acid as substrate. kcat is 0.89 sec(-1) with caffeic acid as substrate. kcat is


enzyme_display_name: UDP-glycosyltransferase 75C1

enzyme: UDP-glycosyltransferase 75C1


organism: Zea mays


substrate_name: APDTRP glycopeptide

substrate: APDTRP glycopeptide


enzyme_display_name: UDP-glycosyltransferase 84A2

enzyme: UDP-glycosyltransferase 84A2


enzyme_display_name: UDP-glycosyltransferase 84A1

enzyme: UDP-glycosyltransferase 84A1


substrate_name: ferulate

substrate: ferulate


substrate_name: caffeate

substrate: caffeate


substrate_name: cinnamate

substrate: cinnamate


enzyme_display_name: UDP-glycosyltransferase 84A3

enzyme: UDP-glycosyltransferase 84A3


substrate_name: abscisic acid

proof_lines:
SlUGT75C1
Solanum lycopersicum
abscisic acid

substrate: abscisic acid


substrate_name: UDP-glucose

column_label: Abstract reports KM values determined for IAA and UDP glucose as 0.8 and 0.7 mM, respectively.

raw_value_unit_evidence: Km raw_value=0.7 mM raw_unit=mM

row_label: Maize IAA glucosyltransferase / UDP glucose

table_label: PubMed XML abstract PMID 33805949

substrate: UDP-glucose


raw_value_unit_evidence: Km raw_value=0.8 mM raw_unit=mM

row_label: Maize IAA glucosyltransferase / indole-3-acetic acid


column_label: Abstract reports the purified enzyme has an apparent Km for UDP-Gal of 630 microm using GalNAc-alpha1-O-phenyl as the acceptor.

raw_value_unit_evidence: Km raw_value=630 microm raw_unit=microM

row_label: Rat core 1 beta3-galactosyltransferase / UDP-Gal donor substrate

table_label: PubMed XML abstract PMID 11673471


enzyme_display_name: UDP-glycosyltransferase 75B1

column_label: Abstract reports apparent K(m) for pABA of 0.12 mm.

raw_value_unit_evidence: Km raw_value=0.12 mM raw_unit=mM

row_label: Arabidopsis UGT75B1 / pABA

table_label: PubMed XML abstract PMID 18385129

enzyme: UDP-glycosyltransferase 75B1


enzyme_display_name: UDP-glycosyltransferase 72E2

substrate_name: coniferyl aldehyde

column_label: Table 2. Summary of the steady-state kinetic parameters of UGTs 72E1 and 72E2; row Coniferyl aldehyde; column 72E2 / K m (mM)

row_label: Coniferyl aldehyde

enzyme: UDP-glycosyltransferase 72E2

substrate: coniferyl aldehyde


substrate_name: sinapyl aldehyde

column_label: Table 2. Summary of the steady-state kinetic parameters of UGTs 72E1 and 72E2; row Sinapyl aldehyde; column 72E2 / K m (mM)

row_label: Sinapyl aldehyde

substrate: sinapyl aldehyde


substrate_name: coniferyl alcohol

column_label: Table 2. Summary of the steady-state kinetic parameters of UGTs 72E1 and 72E2; row Coniferyl alcohol; column 72E2 / K m (mM)

row_label: Coniferyl alcohol

substrate: coniferyl alcohol


substrate_name: sinapyl alcohol

column_label: Table 2. Summary of the steady-state kinetic parameters of UGTs 72E1 and 72E2; row Sinapyl alcohol; column 72E2 / K m (mM)

row_label: Sinapyl alcohol

substrate: sinapyl alcohol


substrate_name: UDP-N-acetyl-alpha-D-galactosamine

substrate: UDP-N-acetyl-alpha-D-galactosamine


organism: Homo sapiens


column_label: Figure 5 row Ferulic acid / UGT72E2; column K_M (mM) = 0.45.

row_label: Ferulic acid / UGT72E2

table_label: Figure 5, Characterization of the UGTs forming 4-O-glucosides with phenylpropanoids


substrate_name: sinapate

column_label: Figure 5 row Sinapic acid / UGT72E2; column K_M (mM) = 0.90.

normalized_value_unit_evidence: Km = 0.9 mM

row_label: Sinapic acid / UGT72E2

substrate: sinapate


enzyme_display_name: UDP-glycosyltransferase 72E3

column_label: Figure 5 row Sinapic acid / UGT72E3; column K_M (mM) = 0.18.

row_label: Sinapic acid / UGT72E3

enzyme: UDP-glycosyltransferase 72E3


column_label: Figure 5 row Sinapyl alcohol / UGT72E3; column K_M (mM) = 0.85.

row_label: Sinapyl alcohol / UGT72E3


organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

substrate_name: UDP-galactose

substrate: UDP-galactose


organism: Spinacia oleracea

substrate_name: UDP-Gal

substrate: UDP-Gal


enzyme_display_name: UniProt Q9UBV7

substrate_name: UDP-alpha-D-galactose

proof_lines:
Raw SKiD Env: Temp=37°C, pH=7
Raw SKiD Env: Temp=-----, pH=7
Raw SKiD Env: Temp=37°C, pH=7.4

enzyme: UniProt Q9UBV7

substrate: UDP-alpha-D-galactose


proof_lines:
Raw SKiD Env: Temp=37°C, pH=7
Raw SKiD Env: Temp=-----, pH=-----


substrate_name: UDP-xylose

substrate: UDP-xylose


enzyme_display_name: procollagen glucosyltransferase

enzyme: procollagen glucosyltransferase


enzyme_display_name: Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3


substrate_name: ascorbate

enzyme: Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3

substrate: ascorbate


substrate_name: phenyl beta-D-galactoside

substrate: phenyl beta-D-galactoside


enzyme_display_name: Beta-1,4-galactosyltransferase 4

substrate_name: GlcNAc

enzyme: Beta-1,4-galactosyltransferase 4

substrate: GlcNAc


organism: Canis lupus familiaris

substrate_name: UDP-N-acetylgalactosamine

substrate: UDP-N-acetylgalactosamine


organism: Mus musculus

substrate_name: GD3(N-acetylneuraminic acid)

substrate: GD3(N-acetylneuraminic acid)


enzyme_display_name: UniProt O30511

organism: Helicobacter pylori

substrate_name: lactose

enzyme: UniProt O30511

substrate: lactose


substrate_name: N-acetyllactosamine

substrate: N-acetyllactosamine


substrate_name: lactosylceramide

substrate: lactosylceramide


substrate_name: gallic acid (at pH 5.0 and 30 degrees Celsius)

assay_conditions_summary: pH 5


substrate_name: 4-hydroxybenzoic acid (at pH 5.0 and 30 degrees Celsius)


substrate_name: 3,4-dihydroxybenzoic acid (at pH 5.0 and 30 degrees Celsius)


substrate_name: caffeic acid (at pH 5.0 and 30 degrees Celsius)


substrate_name: cinnamic acid (at pH 5.0 and 30 degrees Celsius)


substrate_name: coumaric acid (at pH 5.0 and 30 degrees Celsius)


substrate_name: ferulic acid (at pH 5.0 and 30 degrees Celsius)


substrate_name: sinapic acid (at pH 5.0 and 30 degrees Celsius)


substrate: gallic acid (at pH 5.0 and 30 degrees Celsius)


substrate: 4-hydroxybenzoic acid (at pH 5.0 and 30 degrees Celsius)


substrate: 3,4-dihydroxybenzoic acid (at pH 5.0 and 30 degrees Celsius)


substrate: caffeic acid (at pH 5.0 and 30 degrees Celsius)


substrate: cinnamic acid (at pH 5.0 and 30 degrees Celsius)


substrate: coumaric acid (at pH 5.0 and 30 degrees Celsius)


substrate: ferulic acid (at pH 5.0 and 30 degrees Celsius)


substrate: sinapic acid (at pH 5.0 and 30 degrees Celsius)


proof_lines: at site Thr-9, hT2, single turnover event


proof_lines: at site Thr-9, hT2CD, at pre-steady state

proof_lines: at site Thr-9, hT2CD, single turnover event


proof_lines: at site Thr-3, hT2, single turnover event


proof_lines: at site Thr-13, hT2, at pre-steady state


proof_lines:
at site Thr-2 and Thr-12, hT10CD-hT2LD
at sites Thr-2 and Thr12, hT10CD-hT2LD


proof_lines:
at sites Thr-2 and Thr-12, hT10CD
at sites Thr-2 and Thr12, hT10CD


proof_lines:
at site Thr-2 and Thr-12, hT10
at sites Thr-2 and Thr12, hT10


substrate_name: UDP-N-acetyl-D-galactosamine

substrate: UDP-N-acetyl-D-galactosamine


substrate_name: STPSTPSTPSTPSTP

substrate: STPSTPSTPSTPSTP


substrate_name: UDP-galactose

proof_lines: 37°C, mutant enzyme R365K, hydrolase reaction

proof_lines: 37°C, mutant enzyme R365K, galactosyltransferase reaction

substrate: UDP-galactose


proof_lines: 37°C, mutant enzyme Q247E, hydrolase reaction

proof_lines: 37°C, mutant enzyme Q247E, galactosyltransferase reaction


proof_lines: 37°C, wild-type enzyme, hydrolase reaction

proof_lines: wild-type, N-acetyllactosamine, pH 6, 30°C

proof_lines: wild-type, lactose as acceptor, 37°C, MnCl2, 25 mM Tris-HCl, pH 7.0


proof_lines: 37°C, mutant enzyme H280Q, galactosyltransferase reaction


proof_lines: 37°C, wild-type enzyme, galactosyltransferase reaction

proof_lines: wild-type kb, UDP-galactose as donor, lactose as acceptor, 37°C, MnCl2, 25 mM Tris-HCl, pH 7.0


substrate_name: UDP-N-acetyl-alpha-D-galactosamine

substrate: UDP-N-acetyl-alpha-D-galactosamine


enzyme_display_name: sterol 3beta-glucosyltransferase

organism: Withania somnifera

substrate_name: diosgenin

enzyme: sterol 3beta-glucosyltransferase

substrate: diosgenin


substrate_name: beta-sitosterol

proof_lines: activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C

substrate: beta-sitosterol


substrate_name: pregnenolone

substrate: pregnenolone


substrate_name: dehydroepiandrosterone

substrate: dehydroepiandrosterone


substrate_name: UDP-glucose

substrate: UDP-glucose


substrate_name: stigmasterol

substrate: stigmasterol


substrate_name: 24-methylene cholesterol

substrate: 24-methylene cholesterol


enzyme_display_name: sucrose 6F-alpha-galactosyltransferase

organism: Sesamum indicum

substrate_name: UDPgalactose

enzyme: sucrose 6F-alpha-galactosyltransferase

substrate: UDPgalactose


substrate_name: sucrose

substrate: sucrose


substrate_name: ergosterol

substrate: ergosterol


substrate_name: brassicasterol

substrate: brassicasterol


substrate_name: solasodine

substrate: solasodine


organism: Cycas revoluta

substrate_name: methylazoxymethanol

substrate: methylazoxymethanol


substrate_name: UDPglucose

substrate: UDPglucose


substrate_name: UDP-GalNAc

substrate: UDP-GalNAc


proof_lines: GalNac-T1 triple mutant D444A/D484A/D525A


mutation_type: deletion

proof_lines: inhibition of GalNAc-T1 mutant P-deltaN42


enzyme_display_name: isoflavone 7-O-glucosyltransferase

organism: Glycine max

substrate_name: daidzein

proof_lines: pH 8.5, 30°C, recombinant isozyme GmUGT3

proof_lines: pH 8.5, 30°C, recombinant isozyme GmUGT1

enzyme: isoflavone 7-O-glucosyltransferase

substrate: daidzein


proof_lines: purified enzyme, enzyme assay is run at pH 8.5 at 30°C


substrate_name: genistein

substrate: genistein


substrate_name: eriodictyol

column_label: Table 3 row Eriodictyol, column Km (uM), reports 18.0.

raw_value_unit_evidence: Eriodictyol row: Km 18.0 uM; Vmax 333.3 pkat/mg; Vmax/Km 18.5; Kcat/Km 2.66 uM^-1/s.

row_label: Eriodictyol

table_label: PDF Table 3, Substrate Specificities of the Purified AtGT-2 Recombinant Protein

substrate: eriodictyol


substrate_name: kaempferol

column_label: Table 3 row Kaempferol, column Km (uM), reports 26.3.

raw_value_unit_evidence: Kaempferol row: Km 26.3 uM; Vmax 333.3 pkat/mg; Vmax/Km 12.7; Kcat/Km 1.32 uM^-1/s.

row_label: Kaempferol

substrate: kaempferol


substrate_name: quercetin

column_label: Table 3 row Quercetin, column Km (uM), reports 35.3.

raw_value_unit_evidence: Quercetin row: Km 35.3 uM; Vmax 250.0 pkat/mg; Vmax/Km 7.1; Kcat/Km 0.41 uM^-1/s.

row_label: Quercetin

substrate: quercetin


organism: Sinapis alba

substrate_name: sitosterol

substrate: sitosterol


substrate_name: UDP-glucose

substrate: UDP-glucose


enzyme_display_name: xyloglucan 4-glucosyltransferase

enzyme: xyloglucan 4-glucosyltransferase


organism: Gardenia jasminoides


substrate_name: salicyl alcohol

substrate: salicyl alcohol


organism: Badhamia polycephala

substrate_name: poriferasterol

substrate: poriferasterol


organism: Solanum melongena


substrate_name: Genistein; UDPglucose

substrate: Genistein; UDPglucose


organism: Paris polyphylla var. yunnanensis


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature E392A to reconstruct the assayed variant sequence.

proof_lines: E392A mutant, enzyme assay is run at pH 8.5 at 30°C


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature E456A to reconstruct the assayed variant sequence.

proof_lines: E456A mutant, enzyme assay is run at pH 8.5 at 30°C


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature E376A to reconstruct the assayed variant sequence.

proof_lines: E376A mutant, enzyme assay is run at pH 8.5 at 30°C


sequence_variant_note: Mutation signature E392D could not be applied safely because the resolved sequence does not match the expected reference residue(s).

proof_lines: E392D mutant, enzyme assay is run at pH 8.5 at 30°C


enzyme_display_name: Isoflavone 7-O-glucosyltransferase 1

proof_lines: kcat is 0.74 sec(-1)for genistein (at pH 8.5 and 30 degrees Celsius).

enzyme: Isoflavone 7-O-glucosyltransferase 1


organism: Punica granatum

substrate_name: Unknown

proof_lines: kcat is 0.52 sec(-1) with gallic acid as substrate. kcat is 0.61 sec(-1) with 4-hydroxybenzoic acid as substrate. kcat is 0.76 sec(-1) with 3,4-dihydroxybenzoic acid as substrate. kcat is 0.64 sec(-1) with caffeic acid as substrate. kcat is

substrate: Unknown


proof_lines: kcat is 0.55 sec(-1) with gallic acid as substrate. kcat is 0.6 sec(-1) with 4-hydroxybenzoic acid as substrate. kcat is 1.35 sec(-1) with 3,4-dihydroxybenzoic acid as substrate. kcat is 0.89 sec(-1) with caffeic acid as substrate. kcat is


organism: Avena sativa


enzyme_display_name: UDP-glycosyltransferase 1

organism: Pueraria montana var. lobata

proof_lines: kcat is 1.1 sec(-1) with daidzein as substrate. kcat is 1.1 sec(-1) with genistein as substrate.

enzyme: UDP-glycosyltransferase 1


organism: Ogataea angusta

substrate_name: testosterone

column_label: Table 2 row A7KAK6 T gives Km 837.9, kcat 351.9, and kcat/Km 0.42.

normalized_value_unit_evidence: Paper-normalized metrics: kcat 351.9 s^-1; Km 0.8379 mM; kcat/Km 420 s^-1 mM^-1.

row_label: A7KAK6 / testosterone (T)

table_label: Table 2. Kinetic parameters of the UDPG-SGTases

substrate: testosterone


substrate_name: cholesterol

column_label: Table 2 row A7KAK6 C gives Km 67.2, kcat 650.6, and kcat/Km 9.7.

normalized_value_unit_evidence: Paper-normalized metrics: kcat 650.6 s^-1; Km 0.0672 mM; kcat/Km 9700 s^-1 mM^-1.

row_label: A7KAK6 / cholesterol (C)

substrate: cholesterol


enzyme_display_name: Sterol 3-beta-glucosyltransferase

organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

enzyme: Sterol 3-beta-glucosyltransferase


enzyme_display_name: flavanone 7-O-beta-glucosyltransferase

organism: Lycium barbarum

substrate_name: naringenin

enzyme: flavanone 7-O-beta-glucosyltransferase

substrate: naringenin


organism: Arabidopsis thaliana

substrate_name: solasodine

substrate: solasodine


substrate_name: pregnenolone

substrate: pregnenolone


substrate_name: brassicasterol

substrate: brassicasterol


substrate_name: ergosterol

substrate: ergosterol


substrate_name: dehydroepiandrosterone

substrate: dehydroepiandrosterone


substrate_name: stigmasterol

substrate: stigmasterol


substrate_name: beta-sitosterol

substrate: beta-sitosterol


substrate_name: gallic acid (at pH 5.0 and 30 degrees Celsius)

column_label: Table 1. Kinetic parameters of pomegranate UGT84A23 and UGT84A24 towards selected phenolic acid substrates.; row UGT84A23 / Gallic acid; column UGT84A23 Km (mM); cell 0.89 ± 0.07.

normalized_value_unit_evidence: Km normalized_value=0.89 normalized_unit=mM from Table 1 Km (mM); no unit conversion required.

raw_value_unit_evidence: Table 1 row UGT84A23 / Gallic acid: Km 0.89 ± 0.07 mM.

row_label: UGT84A23 / Gallic acid


substrate_name: 4-hydroxybenzoic acid (at pH 5.0 and 30 degrees Celsius)

column_label: Table 1. Kinetic parameters of pomegranate UGT84A23 and UGT84A24 towards selected phenolic acid substrates.; row UGT84A23 / 4-HBA; column UGT84A23 Km (mM); cell 1.19 ± 0.03.

normalized_value_unit_evidence: Km normalized_value=1.19 normalized_unit=mM from Table 1 Km (mM); no unit conversion required.

raw_value_unit_evidence: Table 1 row UGT84A23 / 4-HBA: Km 1.19 ± 0.03 mM.

row_label: UGT84A23 / 4-HBA


substrate_name: 3,4-dihydroxybenzoic acid (at pH 5.0 and 30 degrees Celsius)

column_label: Table 1. Kinetic parameters of pomegranate UGT84A23 and UGT84A24 towards selected phenolic acid substrates.; row UGT84A23 / 3,4-DHBA; column UGT84A23 Km (mM); cell 2.46 ± 0.26.

normalized_value_unit_evidence: Km normalized_value=2.46 normalized_unit=mM from Table 1 Km (mM); no unit conversion required.

raw_value_unit_evidence: Table 1 row UGT84A23 / 3,4-DHBA: Km 2.46 ± 0.26 mM.


substrate_name: caffeic acid (at pH 5.0 and 30 degrees Celsius)

column_label: Table 1. Kinetic parameters of pomegranate UGT84A23 and UGT84A24 towards selected phenolic acid substrates.; row UGT84A23 / Caffeic acid; column UGT84A23 Km (mM); cell 1.06 ± 0.11.

normalized_value_unit_evidence: Km normalized_value=1.06 normalized_unit=mM from Table 1 Km (mM); no unit conversion required.

raw_value_unit_evidence: Table 1 row UGT84A23 / Caffeic acid: Km 1.06 ± 0.11 mM.

row_label: UGT84A23 / Caffeic acid


substrate_name: cinnamic acid (at pH 5.0 and 30 degrees Celsius)

column_label: Table 1. Kinetic parameters of pomegranate UGT84A23 and UGT84A24 towards selected phenolic acid substrates.; row UGT84A23 / Cinnamic acid; column UGT84A23 Km (mM); cell 1.12 ± 0.08.

normalized_value_unit_evidence: Km normalized_value=1.12 normalized_unit=mM from Table 1 Km (mM); no unit conversion required.

raw_value_unit_evidence: Table 1 row UGT84A23 / Cinnamic acid: Km 1.12 ± 0.08 mM.

row_label: UGT84A23 / Cinnamic acid


substrate_name: coumaric acid (at pH 5.0 and 30 degrees Celsius)

column_label: Table 1. Kinetic parameters of pomegranate UGT84A23 and UGT84A24 towards selected phenolic acid substrates.; row UGT84A23 / Coumaric acid; column UGT84A23 Km (mM); cell 0.94 ± 0.13.

normalized_value_unit_evidence: Km normalized_value=0.94 normalized_unit=mM from Table 1 Km (mM); no unit conversion required.

raw_value_unit_evidence: Table 1 row UGT84A23 / Coumaric acid: Km 0.94 ± 0.13 mM.

row_label: UGT84A23 / Coumaric acid


substrate_name: ferulic acid (at pH 5.0 and 30 degrees Celsius)

column_label: Table 1. Kinetic parameters of pomegranate UGT84A23 and UGT84A24 towards selected phenolic acid substrates.; row UGT84A23 / Ferulic acid; column UGT84A23 Km (mM); cell 1.58 ± 0.01.

normalized_value_unit_evidence: Km normalized_value=1.58 normalized_unit=mM from Table 1 Km (mM); no unit conversion required.

raw_value_unit_evidence: Table 1 row UGT84A23 / Ferulic acid: Km 1.58 ± 0.01 mM.

row_label: UGT84A23 / Ferulic acid


substrate_name: sinapic acid (at pH 5.0 and 30 degrees Celsius)

column_label: Table 1. Kinetic parameters of pomegranate UGT84A23 and UGT84A24 towards selected phenolic acid substrates.; row UGT84A23 / Sinapic acid; column UGT84A23 Km (mM); cell 0.86 ± 0.05.

normalized_value_unit_evidence: Km normalized_value=0.86 normalized_unit=mM from Table 1 Km (mM); no unit conversion required.

raw_value_unit_evidence: Table 1 row UGT84A23 / Sinapic acid: Km 0.86 ± 0.05 mM.

row_label: UGT84A23 / Sinapic acid


column_label: Table 1. Kinetic parameters of pomegranate UGT84A23 and UGT84A24 towards selected phenolic acid substrates.; row UGT84A24 / Gallic acid; column UGT84A24 Km (mM); cell 0.98 ± 0.01.

normalized_value_unit_evidence: Km normalized_value=0.98 normalized_unit=mM from Table 1 Km (mM); no unit conversion required.

raw_value_unit_evidence: Table 1 row UGT84A24 / Gallic acid: Km 0.98 ± 0.01 mM.

row_label: UGT84A24 / Gallic acid

substrate: gallic acid (at pH 5.0 and 30 degrees Celsius)


column_label: Table 1. Kinetic parameters of pomegranate UGT84A23 and UGT84A24 towards selected phenolic acid substrates.; row UGT84A24 / 4-HBA; column UGT84A24 Km (mM); cell 1.17 ± 0.03.

normalized_value_unit_evidence: Km normalized_value=1.17 normalized_unit=mM from Table 1 Km (mM); no unit conversion required.

raw_value_unit_evidence: Table 1 row UGT84A24 / 4-HBA: Km 1.17 ± 0.03 mM.

row_label: UGT84A24 / 4-HBA

substrate: 4-hydroxybenzoic acid (at pH 5.0 and 30 degrees Celsius)


column_label: Table 1. Kinetic parameters of pomegranate UGT84A23 and UGT84A24 towards selected phenolic acid substrates.; row UGT84A24 / 3,4-DHBA; column UGT84A24 Km (mM); cell 4.44 ± 0.73.

normalized_value_unit_evidence: Km normalized_value=4.44 normalized_unit=mM from Table 1 Km (mM); no unit conversion required.

raw_value_unit_evidence: Table 1 row UGT84A24 / 3,4-DHBA: Km 4.44 ± 0.73 mM.

substrate: 3,4-dihydroxybenzoic acid (at pH 5.0 and 30 degrees Celsius)


column_label: Table 1. Kinetic parameters of pomegranate UGT84A23 and UGT84A24 towards selected phenolic acid substrates.; row UGT84A24 / Caffeic acid; column UGT84A24 Km (mM); cell 1.77 ± 0.17.

normalized_value_unit_evidence: Km normalized_value=1.77 normalized_unit=mM from Table 1 Km (mM); no unit conversion required.

raw_value_unit_evidence: Table 1 row UGT84A24 / Caffeic acid: Km 1.77 ± 0.17 mM.

row_label: UGT84A24 / Caffeic acid

substrate: caffeic acid (at pH 5.0 and 30 degrees Celsius)


column_label: Table 1. Kinetic parameters of pomegranate UGT84A23 and UGT84A24 towards selected phenolic acid substrates.; row UGT84A24 / Cinnamic acid; column UGT84A24 Km (mM); cell 0.86 ± 0.09.

raw_value_unit_evidence: Table 1 row UGT84A24 / Cinnamic acid: Km 0.86 ± 0.09 mM.

row_label: UGT84A24 / Cinnamic acid

substrate: cinnamic acid (at pH 5.0 and 30 degrees Celsius)


column_label: Table 1. Kinetic parameters of pomegranate UGT84A23 and UGT84A24 towards selected phenolic acid substrates.; row UGT84A24 / Coumaric acid; column UGT84A24 Km (mM); cell 1.06 ± 0.15.

raw_value_unit_evidence: Table 1 row UGT84A24 / Coumaric acid: Km 1.06 ± 0.15 mM.

row_label: UGT84A24 / Coumaric acid

substrate: coumaric acid (at pH 5.0 and 30 degrees Celsius)


column_label: Table 1. Kinetic parameters of pomegranate UGT84A23 and UGT84A24 towards selected phenolic acid substrates.; row UGT84A24 / Ferulic acid; column UGT84A24 Km (mM); cell 1.32 ± 0.02.

normalized_value_unit_evidence: Km normalized_value=1.32 normalized_unit=mM from Table 1 Km (mM); no unit conversion required.

raw_value_unit_evidence: Table 1 row UGT84A24 / Ferulic acid: Km 1.32 ± 0.02 mM.

row_label: UGT84A24 / Ferulic acid

substrate: ferulic acid (at pH 5.0 and 30 degrees Celsius)


column_label: Table 1. Kinetic parameters of pomegranate UGT84A23 and UGT84A24 towards selected phenolic acid substrates.; row UGT84A24 / Sinapic acid; column UGT84A24 Km (mM); cell 0.78 ± 0.02.

normalized_value_unit_evidence: Km normalized_value=0.78 normalized_unit=mM from Table 1 Km (mM); no unit conversion required.

raw_value_unit_evidence: Table 1 row UGT84A24 / Sinapic acid: Km 0.78 ± 0.02 mM.

row_label: UGT84A24 / Sinapic acid

substrate: sinapic acid (at pH 5.0 and 30 degrees Celsius)


substrate_name: Genistein

sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature D125A with inferred residue-numbering offset +6 to reconstruct the assayed variant sequence.

substrate: Genistein


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:A6BM07 and applied mutation signature E376A to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic parameters for the enzyme from the roots of G. max seedlings, recombinant wild-type enzyme, and mutants), enzyme row E376A, Genistein column group; column kcat (s^-1) = 0.63; column Genistein Km (uM) = 4.5; column Genistein kcat/Km (s^-1 uM^-1) = 0.14. captured primary segment: Table 2 | Genistein | GmIF7GT E376A | kcat 0.63 s^-1 | Km 4.5 uM | kcat/Km 0.14 s^-1 uM^-1

normalized_value_unit_evidence: kcat: printed Table 2 cell 0.63 s^-1; the turnover column is printed per second, so no unit conversion applies and the value is 0.63 s^(-1). Km: printed Table 2 Genistein cell 4.5 uM; 1 uM is 1/1000 mM, so 4.5 / 1000 = 0.0045 mM. kcat/Km: printed Table 2 Genistein cell 0.14 s^-1 uM^-1; 1 s^-1 uM^-1 is 1000 s^-1 mM^-1, so 0.14 x 1000 = 140.0 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, enzyme row E376A, Genistein column group: kcat = 0.63 s^-1; Km = 4.5 uM; printed kcat/Km = 0.14 s^-1 uM^-1.

table_label: Table 2: Kinetic parameters for the enzyme from the roots of G. max seedlings, recombinant wild-type enzyme, and mutants (J. Biol. Chem. 2007, 282(32):23581-23590); printed column heads Enzyme | kcat (s^-1) | Genistein Km (uM) | Genistein kcat/Km (s^-1 uM^-1) | UDP-glucose Km (uM) | UDP-glucose kcat/Km (s^-1 mM^-1)


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature E392A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:A6BM07 and applied mutation signature E392D to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic parameters for the enzyme from the roots of G. max seedlings, recombinant wild-type enzyme, and mutants), enzyme row E392D, Genistein column group; column kcat (s^-1) = 3.0; column Genistein Km (uM) = 0.77; column Genistein kcat/Km (s^-1 uM^-1) = 3.9. captured primary segment: Table 2 | Genistein | GmIF7GT E392D | kcat 3.0 s^-1 | Km 0.77 uM | kcat/Km 3.9 s^-1 uM^-1

normalized_value_unit_evidence: kcat: printed Table 2 cell 3.0 s^-1; the turnover column is printed per second, so no unit conversion applies and the value is 3.0 s^(-1). Km: printed Table 2 Genistein cell 0.77 uM; 1 uM is 1/1000 mM, so 0.77 / 1000 = 0.00077 mM. kcat/Km: printed Table 2 Genistein cell 3.9 s^-1 uM^-1; 1 s^-1 uM^-1 is 1000 s^-1 mM^-1, so 3.9 x 1000 = 3900.0 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, enzyme row E392D, Genistein column group: kcat = 3.0 s^-1; Km = 0.77 uM; printed kcat/Km = 3.9 s^-1 uM^-1.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:A6BM07 and applied mutation signature E456A to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic parameters for the enzyme from the roots of G. max seedlings, recombinant wild-type enzyme, and mutants), enzyme row E456A, Genistein column group; column kcat (s^-1) = 0.41; column Genistein Km (uM) = 2.6; column Genistein kcat/Km (s^-1 uM^-1) = 0.16. captured primary segment: Table 2 | Genistein | GmIF7GT E456A | kcat 0.41 s^-1 | Km 2.6 uM | kcat/Km 0.16 s^-1 uM^-1

normalized_value_unit_evidence: kcat: printed Table 2 cell 0.41 s^-1; the turnover column is printed per second, so no unit conversion applies and the value is 0.41 s^(-1). Km: printed Table 2 Genistein cell 2.6 uM; 1 uM is 1/1000 mM, so 2.6 / 1000 = 0.0026 mM. kcat/Km: printed Table 2 Genistein cell 0.16 s^-1 uM^-1; 1 s^-1 uM^-1 is 1000 s^-1 mM^-1, so 0.16 x 1000 = 160.0 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, enzyme row E456A, Genistein column group: kcat = 0.41 s^-1; Km = 2.6 uM; printed kcat/Km = 0.16 s^-1 uM^-1.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature H15A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:A6BM07 and applied mutation signature H359A to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic parameters for the enzyme from the roots of G. max seedlings, recombinant wild-type enzyme, and mutants), enzyme row H359A, Genistein column group; column kcat (s^-1) = 0.59; column Genistein Km (uM) = 2.7; column Genistein kcat/Km (s^-1 uM^-1) = 0.22. captured primary segment: Table 2 | Genistein | GmIF7GT H359A | kcat 0.59 s^-1 | Km 2.7 uM | kcat/Km 0.22 s^-1 uM^-1

normalized_value_unit_evidence: kcat: printed Table 2 cell 0.59 s^-1; the turnover column is printed per second, so no unit conversion applies and the value is 0.59 s^(-1). Km: printed Table 2 Genistein cell 2.7 uM; 1 uM is 1/1000 mM, so 2.7 / 1000 = 0.0027 mM. kcat/Km: printed Table 2 Genistein cell 0.22 s^-1 uM^-1; 1 s^-1 uM^-1 is 1000 s^-1 mM^-1, so 0.22 x 1000 = 220.0 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, enzyme row H359A, Genistein column group: kcat = 0.59 s^-1; Km = 2.7 uM; printed kcat/Km = 0.22 s^-1 uM^-1.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:A6BM07 and applied mutation signature H368A to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic parameters for the enzyme from the roots of G. max seedlings, recombinant wild-type enzyme, and mutants), enzyme row H368A, Genistein column group; column kcat (s^-1) = 2.0; column Genistein Km (uM) = 4.9; column Genistein kcat/Km (s^-1 uM^-1) = 0.41. captured primary segment: Table 2 | Genistein | GmIF7GT H368A | kcat 2.0 s^-1 | Km 4.9 uM | kcat/Km 0.41 s^-1 uM^-1

normalized_value_unit_evidence: kcat: printed Table 2 cell 2.0 s^-1; the turnover column is printed per second, so no unit conversion applies and the value is 2.0 s^(-1). Km: printed Table 2 Genistein cell 4.9 uM; 1 uM is 1/1000 mM, so 4.9 / 1000 = 0.0049 mM. kcat/Km: printed Table 2 Genistein cell 0.41 s^-1 uM^-1; 1 s^-1 uM^-1 is 1000 s^-1 mM^-1, so 0.41 x 1000 = 410.0 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, enzyme row H368A, Genistein column group: kcat = 2.0 s^-1; Km = 4.9 uM; printed kcat/Km = 0.41 s^-1 uM^-1.


substrate_name: UDPglucose

substrate: UDPglucose


column_label: Table 2 row E376A, substrate UDP-glucose; raw cells kcat 0.63 +/- 0.01 s^-1, Km 2100 +/- 130 uM, kcat/Km 0.30 s^-1*mM^-1; normalized cells kcat 0.63 s^-1, Km 2.1 mM, kcat/Km 0.30 s^-1*mM^-1.

normalized_value_unit_evidence: kcat normalized_value=0.63 normalized_unit=s^-1; Km normalized_value=2.1 normalized_unit=mM; kcat/Km normalized_value=0.30 normalized_unit=s^-1*mM^-1; UDP-glucose catalytic efficiency printed in s^-1*mM^-1.

raw_value_unit_evidence: kcat raw_value=0.63 +/- 0.01 raw_unit=s^-1; Km raw_value=2100 +/- 130 raw_unit=uM; kcat/Km raw_value=0.30 raw_unit=s^-1*mM^-1

row_label: E376A / UDP-glucose

table_label: Table 2. Kinetic parameters for the enzyme from the roots of G. max seedlings, recombinant wild-type enzyme, and mutants


substrate_name: cholesterol

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

column_label: Table 2 row A7KAK6 C gives the exact cholesterol kinetic tuple.

normalized_value_unit_evidence: Paper-normalized metrics: kcat 650.6 s^-1; Km 0.0672 mM; kcat/Km 9700 s^-1 mM^-1.

substrate: cholesterol


substrate_name: testosterone

column_label: Table 2 row A7KAK6 T gives the exact testosterone kinetic tuple.

normalized_value_unit_evidence: Paper-normalized metrics: kcat 351.9 s^-1; Km 0.8379 mM; kcat/Km 420 s^-1 mM^-1.

row_label: A7KAK6 / testosterone (T)

substrate: testosterone


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature E392D to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature E456A with inferred residue-numbering offset -4 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature H359A with inferred residue-numbering offset +9 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature H368A with inferred residue-numbering offset -9 to reconstruct the assayed variant sequence.


organism: Kluyveromyces lactis

column_label: Table 2 row Q6CUV2 C gives Km 65.92, kcat 475.0, and kcat/Km 7.21.

normalized_value_unit_evidence: Paper-normalized metrics: kcat 475.0 s^-1; Km 0.06592 mM; kcat/Km 7210 s^-1 mM^-1.

row_label: Q6CUV2 / cholesterol (C)


organism: Bos taurus

substrate_name: UDP-galactose

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: AGGL mutant, N-acetyllactosamine, pH 6, 30°C

proof_lines: mutant AGG, lactose as acceptor, 37°C, MnCl2, 25 mM Tris-HCl, pH 7.0

substrate: UDP-galactose


substrate_name: UDP-N-acetylgalactosamine

substrate: UDP-N-acetylgalactosamine


substrate_name: N-acetyllactosamine

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

substrate: N-acetyllactosamine


proof_lines: AGGL mutant, UDP-N-acetylgalactosamine, pH 6, 30°C


substrate_name: lactose

proof_lines: mutant SGG kb, UDP-galactose as donor, lactose as acceptor, 37°C, MnCl2, 25 mM Tris-HCl, pH 7.0

substrate: lactose


enzyme_display_name: type 1 galactoside alpha-(1,2)-fucosyltransferase

organism: Homo sapiens

substrate_name: lacto-N-tetraose

proof_lines:
Se-gene encoded enzyme
H-gene encoded enzyme

substrate: lacto-N-tetraose


enzyme: type 1 galactoside alpha-(1,2)-fucosyltransferase


proof_lines:
H-gene encoded enzyme
Se-gene encoded enzyme


substrate_name: beta-D-galactose

substrate: beta-D-galactose


substrate_name: lacto-N-neotetraose

substrate: lacto-N-neotetraose


organism: Mus musculus

substrate_name: GDP-L-fucose

proof_lines: reaction with phenyl-beta-D-galactoside, recombinant enzyme

substrate: GDP-L-fucose


substrate_name: lactosylceramide

substrate: lactosylceramide


substrate_name: lacto-N-biose

substrate: lacto-N-biose


enzyme_display_name: UniProt E8MF13

organism: Bifidobacterium longum

substrate_name: lacto-N-biose I

enzyme: UniProt E8MF13

substrate: lacto-N-biose I


substrate_name: alpha-D-galactose 1-phosphate

substrate: alpha-D-galactose 1-phosphate


substrate_name: galacto-N-biose

sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature P161S to reconstruct the assayed variant sequence.

substrate: galacto-N-biose


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature P161S/S336A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature S336A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature V162T with inferred residue-numbering offset -11 to reconstruct the assayed variant sequence.


enzyme_display_name: Hyaluronan synthase

organism: Pasteurella multocida

substrate_name: UDP-N-acetyl-D-glucosamine

enzyme: Hyaluronan synthase

substrate: UDP-N-acetyl-D-glucosamine


substrate_name: UDP-D-glucuronate

substrate: UDP-D-glucuronate


substrate_name: GDP-fucose

substrate: GDP-fucose


substrate_name: phenyl beta-D-galactoside

substrate: phenyl beta-D-galactoside


organism: Rattus norvegicus

proof_lines:
enzyme FTB
pH 7.2, 37°C
enzyme FTA


proof_lines:
enzyme FTA
enzyme FTB


proof_lines:
pH 7.2, 37°C
enzyme FTA
enzyme FTB


substrate_name: phenyl beta-D-galactopyranoside

substrate: phenyl beta-D-galactopyranoside


organism: Glycine max

substrate_name: genistein

proof_lines: H15A mutant, enzyme assay is run at pH 8.5 at 30°C

substrate: genistein


proof_lines: H359A muant, enzyme assay is run at pH 8.5 at 30°C


proof_lines: D125A mutant, enzyme assay is run at pH 8.5 at 30°C


proof_lines: wild-type, enzyme assay is run at pH 8.5 at 30°C


proof_lines: H368A mutant, enzyme assay is run at pH 8.5 at 30°C


substrate_name: UDP-glucose

substrate: UDP-glucose


enzyme_display_name: UniProt B7SY86

organism: Rubrobacter xylanophilus

substrate_name: GDP-mannose

enzyme: UniProt B7SY86

substrate: GDP-mannose


enzyme_display_name: vomilenine glucosyltransferase

organism: Rauvolfia serpentina

substrate_name: Vomilenine

enzyme: vomilenine glucosyltransferase

substrate: Vomilenine


enzyme_display_name: UniProt A0A2R2JFJ4

organism: Persicaria tinctoria

enzyme: UniProt A0A2R2JFJ4


substrate_name: p-nitrophenyl-alpha-D-galactoside

substrate: p-nitrophenyl-alpha-D-galactoside


substrate_name: D-galactose

substrate: D-galactose


substrate_name: N-acetyllactosamine

substrate: N-acetyllactosamine


organism: Cicer arietinum

substrate_name: Biochanin A

substrate: Biochanin A


substrate_name: formononetin

substrate: formononetin


substrate_name: UDPglucose

substrate: UDPglucose


substrate_name: UDP-GalNAc

substrate: UDP-GalNAc


proof_lines:
wild-type
wild-type, pH 7.0, 37°C


enzyme_display_name: kojibiose phosphorylase

organism: Thermoanaerobacter brockii

substrate_name: beta-D-glucose 1-phosphate

enzyme: kojibiose phosphorylase

substrate: beta-D-glucose 1-phosphate


substrate_name: IgA hinge

proof_lines: +/-0.01 mM, mutant GalNAc-T2-D541A, pH7.4

proof_lines: +/-0.003 mM, mutant GalNAc-T2-D541A, pH7.4

proof_lines: +/-0.010 mM, mutant GalNAc-T2-D541A, pH7.4

substrate: IgA hinge


proof_lines: +/-0.001 mM, mutant GalNAc-T2-D541A, pH7.4


substrate_name: UDP-Gal

proof_lines: +/-0.005 mM, mutant GalNAc-T2-D541A, pH7.4

substrate: UDP-Gal


enzyme_display_name: cinnamate beta-D-glucosyltransferase

organism: Unknown

substrate_name: Unknown

enzyme: cinnamate beta-D-glucosyltransferase

substrate: Unknown


organism: Flammulina velutipes

substrate_name: alpha-D-glucose

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

substrate: alpha-D-glucose


substrate_name: phosphate

substrate: phosphate


substrate_name: alpha-D-glucose 1-phosphate

substrate: alpha-D-glucose 1-phosphate


substrate_name: alpha,alpha-trehalose

substrate: alpha,alpha-trehalose


organism: Lens culinaris

proof_lines:
with D-pinitol
with raffinose


substrate_name: raffinose

substrate: raffinose


substrate_name: D-pinitol

substrate: D-pinitol


organism: Sus scrofa


enzyme_display_name: alpha,alpha-trehalose synthase

organism: Pyrococcus horikoshii

substrate_name: ADP-alpha-D-glucose

enzyme: alpha,alpha-trehalose synthase

substrate: ADP-alpha-D-glucose


substrate_name: UDP-alpha-D-glucose

substrate: UDP-alpha-D-glucose


substrate_name: GDP-alpha-D-glucose

substrate: GDP-alpha-D-glucose


substrate_name: UDP

substrate: UDP


substrate_name: GDP

substrate: GDP


substrate_name: ADP

substrate: ADP


substrate_name: D-glucose

substrate: D-glucose


enzyme_display_name: sinapate 1-glucosyltransferase

organism: Brassica napus

substrate_name: Sinapic acid

enzyme: sinapate 1-glucosyltransferase

substrate: Sinapic acid


substrate_name: UDP-glucose

substrate: UDP-glucose


substrate_name: ferulate

substrate: ferulate


substrate_name: caffeate

substrate: caffeate


enzyme_display_name: N-acetyllactosamine synthase

organism: Bos taurus

substrate_name: UDP-galactose

enzyme: N-acetyllactosamine synthase

substrate: UDP-galactose


enzyme_display_name: UniProt A0A0H2URG7

organism: Streptococcus pneumoniae TIGR4

substrate_name: UDP-GlcNAc

enzyme: UniProt A0A0H2URG7

substrate: UDP-GlcNAc


enzyme_display_name: UniProt Q7KJA9

organism: Drosophila melanogaster

enzyme: UniProt Q7KJA9


substrate_name: UDPgalactose

proof_lines: at 37°C, pH not specified in the publication

substrate: UDPgalactose


substrate_name: N-acetylglucosamine

smiles: CC(=O)NC(C=O)C(C(C(CO)O)O)O

substrate: N-acetylglucosamine


substrate_name: GDP-L-fucose

substrate: GDP-L-fucose


proof_lines: in the absence of bovine serum albumin, at pH 7.0 and 30°C

proof_lines: in the presence of 12.5 mg/ml bovine serum albumin, at pH 7.0 and 30°C


substrate_name: N-acetyl-D-glucosamine

substrate: N-acetyl-D-glucosamine


enzyme_display_name: nigerose phosphorylase

organism: Lachnoclostridium phytofermentans

enzyme: nigerose phosphorylase


substrate_name: beta-D-glucopyranose 1-phosphate

substrate: beta-D-glucopyranose 1-phosphate


substrate_name: D-galactose

substrate: D-galactose


substrate_name: methyl alpha-D-glucopyranoside

substrate: methyl alpha-D-glucopyranoside


substrate_name: D-Xylose

substrate: D-Xylose


enzyme_display_name: UniProt Q5LH68

organism: Bacteroides fragilis

enzyme: UniProt Q5LH68


organism: Drosophila sp. (in: flies)

substrate_name: UDP-N-acetyl-D-galactosamine

substrate: UDP-N-acetyl-D-galactosamine


substrate_name: Unknown

substrate: Unknown


substrate_name: immunoglobulin G heavy chain

substrate: immunoglobulin G heavy chain


substrate_name: fetuin

substrate: fetuin


enzyme_display_name: cyanidin 3-O-galactosyltransferase

organism: Apium graveolens

substrate_name: cyanidin

enzyme: cyanidin 3-O-galactosyltransferase

substrate: cyanidin


organism: Daucus carota

substrate_name: pelargonidin

substrate: pelargonidin


substrate_name: peonidin

substrate: peonidin


substrate_name: UDP-alpha-D-galactose

proof_lines:
cosubstrate cyanidin, pH 8.6, 30°C
cosubstrate pelargonidin, pH 8.6, 30°C
cosubstrate peonidin, pH 8.6, 30°C

substrate: UDP-alpha-D-galactose


organism: Leishmania major

substrate_name: GDP-alpha-D-glucopyranose

substrate: GDP-alpha-D-glucopyranose


substrate_name: UDP-alpha-D-galactopyranose

substrate: UDP-alpha-D-galactopyranose


substrate_name: UDP-alpha-D-galactofuranose

substrate: UDP-alpha-D-galactofuranose


organism: Vitis vinifera

substrate_name: delphinidin 3-O-beta-D-glucoside

substrate: delphinidin 3-O-beta-D-glucoside


substrate_name: malvidin 3-O-beta-D-glucoside

substrate: malvidin 3-O-beta-D-glucoside


substrate_name: cyanidin 3-O-beta-D-glucoside

substrate: cyanidin 3-O-beta-D-glucoside


substrate_name: petunidin 3-O-beta-D-glucoside

substrate: petunidin 3-O-beta-D-glucoside


substrate_name: pelargonidin 3-O-beta-D-glucoside

substrate: pelargonidin 3-O-beta-D-glucoside


substrate_name: peonidin 3-O-beta-D-glucoside

substrate: peonidin 3-O-beta-D-glucoside


substrate_name: quercetin 3-O-beta-D-glucoside

substrate: quercetin 3-O-beta-D-glucoside


enzyme_display_name: UniProt Q21MB1

organism: Saccharophagus degradans

substrate_name: D-gluconate

enzyme: UniProt Q21MB1

substrate: D-gluconate


substrate_name: D-glucuronate

substrate: D-glucuronate


enzyme_display_name: UniProt Q9AFC7

organism: Amycolatopsis orientalis

substrate_name: desvancosaminyl-vancomycin

enzyme: UniProt Q9AFC7

substrate: desvancosaminyl-vancomycin


organism: Petunia x hybrida


substrate_name: UDP-alpha-D-glucose

substrate: UDP-alpha-D-glucose


substrate_name: UDP-alpha-D-glucopyranose

substrate: UDP-alpha-D-glucopyranose


substrate_name: GDP-alpha-D-mannopyranose

substrate: GDP-alpha-D-mannopyranose


enzyme_display_name: UniProt D6XZ22

organism: Salisediminibacterium selenitireducens

substrate_name: beta-D-glucose 1-phosphate

enzyme: UniProt D6XZ22

substrate: beta-D-glucose 1-phosphate


enzyme_display_name: 1,3-alpha-oligoglucan phosphorylase

enzyme: 1,3-alpha-oligoglucan phosphorylase


substrate_name: beta-D-glucopyranose 1-phosphate

substrate: beta-D-glucopyranose 1-phosphate


substrate_name: kojibiose

substrate: kojibiose


substrate_name: maltose

substrate: maltose


organism: Cyclamen purpurascens


organism: Trichormus variabilis (strain ATCC 29413 / PCC 7937)

substrate_name: UDP-Glc

substrate: UDP-Glc


organism: Streptomyces kanamyceticus

proof_lines: 37°C, pH not specified in the publication, cosubstrate: paromamine

proof_lines: kcat is 3.36 min(-1) and 3.18 min(-1) for the reaction with paromamine as acceptor and UDP-alpha-D-kanosamine or UDP-alpha-D-glucose as donor, respectively.


substrate_name: UDP-alpha-D-kanosamine

proof_lines: 37°C, pH not specified in the publication

substrate: UDP-alpha-D-kanosamine