substrate_name: p-nitrophenyl-alpha-L-arabinofuranose

mutation_signature: Tx-Abf-M94_I103delinsGG mutant

mutation_type: delins

proof_lines:
Tx-Abf-M94_I103delinsGG mutant, 60°C
Tx-Abf-M94_I103delinsGG mutant

substrate: p-nitrophenyl-alpha-L-arabinofuranose


organism: Thermobacillus xylanilyticus

source_db: brenda

ki_display: Not reported

kcat_over_km_display: Not reported

protein_accession_database: UniProt

source_license: CC BY 4.0

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution

proof_lines:
Tx-Abf-C180A mutant, 60°C
Tx-Abf-C180A mutant

source: brenda


mutation_signature: Unknown

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


proof_lines:
Tx-Abf-W99A mutant, 60°C
Tx-Abf-W99A mutant


substrate_name: 4-nitrophenyl alpha-L-arabinofuranoside

substrate: 4-nitrophenyl alpha-L-arabinofuranoside


organism: Thermothielavioides terrestris


organism: uncultured rumen bacterium

assay_conditions_summary: pH 7; 313.15 K; buffer pH 7

proof_lines: in 50 mM phosphate/citrate buffer pH 7.0, at 40°C


organism: Weissella sp. 142


enzyme_display_name: flavivirin

organism: dengue virus type 1

enzyme: flavivirin


substrate_name: Unknown

kcat_display: Not reported

km_display: Not reported

ki_unit: mM

proof_lines: pH and temperature not specified in the publication

substrate: Unknown


organism: dengue virus type 2

proof_lines: pH 8.5, 37°C, recombinant linked form mutant S75C


proof_lines: pH 8.5, 37°C, recombinant unlinked form mutant K117A

proof_lines: pH 8.5, 37°C, recombinant linked form mutant K117A


proof_lines: pH 8.5, 37°C, recombinant linked form wild-type enzyme

proof_lines: pH 8.5, 37°C, recombinant unlinked form wild-type enzyme


proof_lines: pH 8.5, 37°C, recombinant linked form mutant K117C


proof_lines: pH 8.5, 37°C, recombinant unlinked form mutant A125C/V162C

proof_lines: pH 8.5, 37°C, recombinant linked form mutant A125C/V162C


proof_lines: pH 8.5, 37°C, recombinant unlinked form mutant I73C/P106C

proof_lines: pH 8.5, 37°C, recombinant linked form mutant I73C/P106C


proof_lines: pH 8.5, 37°C, recombinant linked form mutant K117R


proof_lines: pH 8.5, 37°C, recombinant unlinked form mutant S75C/K117C

proof_lines: pH 8.5, 37°C, recombinant linked form mutant S75C/K117C


proof_lines: pH 8.5, temperature not specified in the publication


organism: dengue virus type 3


organism: dengue virus type 4


organism: Langat virus


organism: Selenomonas ruminantium

substrate_name: p-nitrophenyl-alpha-L-arabinofuranoside

substrate: p-nitrophenyl-alpha-L-arabinofuranoside


enzyme_display_name: catalase peroxidase

organism: Mycobacterium tuberculosis

enzyme: catalase peroxidase


proof_lines: mutant enzyme E291K/E292A, at pH 5.0 and 23°C


organism: Escherichia coli

proof_lines: peroxidase activity, mutant enzyme H106C, at pH 6.0 and 25°C

proof_lines: catalase activity, mutant enzyme H106C, at pH 7.0 and 37°C


proof_lines: peroxidase activity, mutant enzyme H106Y, at pH 6.0 and 25°C

proof_lines: catalase activity, mutant enzyme H106Y, at pH 7.0 and 37°C


proof_lines: catalase activity, mutant enzyme H267Y, at pH 7.0 and 37°C


proof_lines: catalase activity, mutant enzyme R102K, at pH 7.0 and 37°C

proof_lines: peroxidase activity, mutant enzyme R102K, at pH 6.0 and 25°C


proof_lines: peroxidase activity, mutant enzyme R102L, at pH 6.0 and 25°C

proof_lines: catalase activity, mutant enzyme R102L, at pH 7.0 and 37°C


proof_lines: catalase activity, mutant enzyme R102C, at pH 7.0 and 37°C

proof_lines: peroxidase activity, mutant enzyme R102C, at pH 6.0 and 25°C


proof_lines: catalase activity, mutant enzyme W105L, at pH 7.0 and 37°C

proof_lines: peroxidase activity, mutant enzyme W105L, at pH 6.0 and 25°C


proof_lines: catalase activity, mutant enzyme W105F, at pH 7.0 and 37°C

proof_lines: peroxidase activity, mutant enzyme W105F, at pH 6.0 and 25°C


organism: Trichoderma koningii


proof_lines:
pH 6.5, wild-type enzyme
pH 4.1, 25°C, wild-type enzyme


proof_lines:
pH 6.5, mutant enzyme D299G
pH 4.1, 25°C, mutant enzyme D299G


proof_lines: recombinant mutants L634F and D735A, 23°C


organism: Alkhumra hemorrhagic fever virus

proof_lines: chimeric proteases with the NS2B cofactor from Langat virus replacing the Alkhurma virus sequence


organism: Synechocystis sp.


assay_conditions_summary: pH 7; 303.15 K; buffer 100 mM phosphate

proof_lines: catalase activity, mutant enzyme D152S, in 100 mM phosphate buffer, pH 7.0, at 23°C

proof_lines: mutant enzyme D152S, catalase activity, pH and temperature not specified in the publication


proof_lines: mutant R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C


proof_lines: mutant Y697A, N- and C-terminal domain separately expressed, pH 7.0, 23°C


proof_lines: mutant E695A, N- and C-terminal domain separately expressed, pH 7.0, 23°C


proof_lines: mutant L690A/R691A, N- and C-terminal domain separately expressed, pH 7.0, 23°C


assay_conditions_summary: pH 7; 303.15 K; buffer several

proof_lines: catalase activity, wild type enzyme, in 100 mM phosphate buffer, pH 7.0, at 23°C

proof_lines: catalase activity, wild type enzyme, in 50 mM phosphate buffer, pH 7.0, and 30°C


organism: Streptomyces avermitilis

proof_lines: wild type enzyme, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C


proof_lines: mutant enzyme N159L, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C


proof_lines: mutant enzyme L289A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C


proof_lines: mutant enzyme N159A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C


proof_lines: mutant enzyme Y192A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C


proof_lines: mutant enzyme D20N, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C


organism: Synechocystis sp. PCC 6803


mutation_type: deletion


proof_lines: mutant DELTA200-214, catalase activity, pH 7.0, 23°C


organism: Aspergillus luchuensis


assay_conditions_summary: pH 7; 296.15 K; buffer 100 mM phosphate

proof_lines: catalase activity, mutant enzyme R439A, in 100 mM phosphate buffer, pH 7.0, at 23°C


proof_lines: catalase activity, mutant enzyme Y249F, in 100 mM phosphate buffer, pH 7.0, at 23°C


proof_lines: catalase activity, mutant enzyme H123E, in 100 mM phosphate buffer, pH 7.0, at 23°C


assay_conditions_summary: pH 6; 298.15 K; buffer 10 mM phosphate

proof_lines: peroxidatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C

proof_lines: catalatic activity, mutant enzyme W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C


proof_lines: peroxidatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C

proof_lines: catalatic activity, mutant enzyme H108L, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C


proof_lines: catalatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C

proof_lines: peroxidatic activity, mutant enzyme W107F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C


proof_lines: catalatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C

proof_lines: peroxidatic activity, mutant enzyme W107F/W321F, in 10 mM KH2PO4/K2HPO4, pH 6.0, at 25°C


organism: Yellow fever virus


assay_conditions_summary: pH 5; 296.15 K; buffer pH 5

proof_lines: wild type enzyme, peroxidase activity, in 50 mM acetate buffer, pH 5.0, 23°C

proof_lines: peroxidase activity, wild type enzyme, in 50 mM acetate buffer pH 5.0, at 23°C


assay_conditions_summary: pH 5; 296.15 K; buffer 50 mM acetate

proof_lines: mutant enzyme Y111A, peroxidase activity, in 50 mM acetate buffer, pH 5.0, 23°C

proof_lines: mutant enzyme Y111A, catalase activity, in 100 mM phosphate buffer, pH 7.0, 23°C


substrate_name: o-Dianisidine

assay_conditions_summary: pH 7.5; 310.15 K; buffer 50 mM sodium phosphate

proof_lines: mutant enzyme S315T, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C

proof_lines: mutant enzyme S315T, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C

proof_lines: mutant enzyme S315T, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C

substrate: o-Dianisidine


proof_lines: wild type enzyme, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C

proof_lines: wild-type, N- and C-terminal domain separately expressed, pH 7.0, 23°C

proof_lines: catalase activity, wild type enzyme, in 100 mM phosphate buffer, pH 7.0, at 23°C


assay_conditions_summary: pH 7; 303.15 K; buffer phosphate

proof_lines: catalase activity, mutant enzyme N153A, in 50 mM phosphate buffer, pH 7.0, and 30°C


proof_lines: catalase activity, mutant enzyme N153D, in 50 mM phosphate buffer, pH 7.0, and 30°C


organism: Geobacillus stearothermophilus

substrate_name: 2,5-dinitrophenyl beta-D-xylopyranoside

substrate: 2,5-dinitrophenyl beta-D-xylopyranoside


organism: Cellvibrio japonicus


substrate_name: arabinobiose

substrate: arabinobiose


organism: West Nile virus

assay_conditions_summary: pH 8; 295.15 K; buffer 20 mM Tris/HCl

proof_lines: mutant enzyme K48A, in 20 mM Tris/HCl buffer, pH 8.0, at 22°C


proof_lines: wild type enzyme, in 20 mM Tris/HCl buffer, pH 8.0, at 22°C


organism: Archaeoglobus fulgidus

proof_lines: catalase reaction, at pH 5.5-6.0 and 37°C


organism: Bacillus pumilus


organism: Bradyrhizobium japonicum

proof_lines: catalase activity, pH and temperature not specified in the publication


organism: Burkholderia pseudomallei


proof_lines: peroxidase activity, mutant enzyme KatGDELTAFG, in 50 mM acetate buffer pH 5.0, at 23°C

proof_lines: catalase activity, mutant enzyme KatGDELTAFG, 100 mM phosphate buffer pH 7.0, at 23°C


substrate_name: pyrogallol

assay_conditions_summary: pH 4; 296.15 K; buffer 50 mM acetate

proof_lines:
pH 4.0, 23°C
peroxidase reaction, at pH 4.3 and 25°C
pH 6.0, 23°C

substrate: pyrogallol


organism: Haloarcula marismortui

proof_lines:
peroxidatic activity, pH 6.0, 25°C
catalatic activity, pH 7.5, 25°C


organism: Halobacterium salinarum

proof_lines: catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C

proof_lines: apparent value, peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C


organism: Klebsiella pneumoniae

proof_lines:
peroxidase activity, pH 5.3, 30°C
catalase activity, pH 6.3, 30°C


organism: Mycobacterium sp.

assay_conditions_summary: pH 7; 303.15 K; buffer 50 mM potassium phosphate

proof_lines: peroxidase activity, in 50 mM potassium phosphate buffer (pH 7.0), at 30°C

proof_lines: catalase activity, in 50 mM potassium phosphate buffer (pH 7.0), at 30°C


organism: Mycobacterium tuberculosis

proof_lines: peroxidatic activity, using tert-butyl hydroperoxide as cosubstrate, in 10 mM K2HPO4/KH2PO4 (pH 7.5), at 25°C


enzyme_display_name: heme oxygenase (biliverdin-producing, ferredoxin)

organism: Arabidopsis thaliana

substrate_name: hemin

proof_lines:
at pH 7.2 and 25°C
isoform HY1, at pH 7.2 and 25°C
isoform HO4, at pH 7.2 and 25°C

substrate: hemin


organism: Rhodobacter capsulatus


organism: Synechococcus elongatus PCC 7942 = FACHB-805

assay_conditions_summary: pH 7; 298.15 K; buffer 67 mM phosphate

proof_lines: catalase activity, in 67 mM phosphate buffer (pH 7.0), at 25°C


organism: Synechocystis sp.

assay_conditions_summary: pH 4.3; 298.15 K; buffer 67 mM phosphate


proof_lines: mutant enzyme E243Q, catalase activity, pH and temperature not specified in the publication


enzyme_display_name: acyl-lipid (8-3)-desaturase

organism: Homo sapiens

substrate_name: dihomo-gamma-linolenic acid

enzyme: acyl-lipid (8-3)-desaturase

substrate: dihomo-gamma-linolenic acid


organism: Rattus norvegicus

proof_lines: pH and temperature not specified in the publication


proof_lines: mutant enzyme R463L, catalase activity, in 50 mM Na2HPO4, pH 7.0, 25°C


organism: Mycolicibacterium smegmatis

substrate_name: NADPH

assay_conditions_summary: pH 7; 298.15 K; buffer 50 mM phosphate; cofactor NADPH

proof_lines: peroxidatic activity, in 50 mM phosphate buffer, pH 7.0, at 25°C

proof_lines: catalatic activity, in 50 mM phosphate buffer, pH 7.0, at 25°C

proof_lines: peroxidatic activity, with hydrogen peroxide as cosubstrate, in 50 mM phosphate buffer, pH 7.0, at 25°C

substrate: NADPH


organism: Oceanobacillus oncorhynchi subsp. incaldanensis

assay_conditions_summary: pH 9; 303.15 K; buffer 20 mM Tris-HCl

proof_lines: catalase activity, in 20 mM Tris-HCl buffer (pH 9.0), at 30°C


organism: Penicillium simplicissimum


organism: Aspergillus fumigatus

proof_lines:
isoform AbfII, at pH 5.0 and 42°C
isoform AbfI, at pH 5.0 and 37°C


organism: Aspergillus nidulans


organism: Aspergillus niger

substrate_name: beet arabinan

proof_lines:
strain B
alpha-L-arabinofuranosidase B
alpha-L-arabinofuranosidase A

substrate: beet arabinan


organism: Bacillus pumilus


organism: Bifidobacterium breve


organism: Butyrivibrio fibrisolvens


organism: Caldicellulosiruptor saccharolyticus

assay_conditions_summary: pH 5.5; 353.15 K; buffer 50 mM citric acid

proof_lines: in 50 mM citric acid buffer (pH 5.5), at 80°C


organism: Caldicellulosiruptor sp. F32


organism: Acetivibrio clariflavus


organism: Acetivibrio thermocellus


organism: Agroathelia rolfsii

substrate_name: phenyl alpha-L-arabinofuranoside

substrate: phenyl alpha-L-arabinofuranoside


organism: Arabidopsis thaliana


organism: Cellulosimicrobium aquatile

substrate_name: ginsenoside Rc

substrate: ginsenoside Rc


organism: Clostridium acetobutylicum


organism: Dichomitus squalens


organism: Fusarium graminearum


organism: Linum usitatissimum

proof_lines: in 0.1 M sodium acetate, pH 6 at 40°C for 30 min


organism: Monilinia fructigena

substrate_name: alpha-L-arabinofuranosyl 4-phenylphenol

substrate: alpha-L-arabinofuranosyl 4-phenylphenol


organism: Paenibacillus sp.


organism: Penicillium chrysogenum

proof_lines:
enzyme AFQ1, pH 5.0, 37°C
enzyme AFS1, pH 5.0, 37°C


organism: Saccharococcus caldoxylosilyticus


organism: Spinacia oleracea

proof_lines:
alpha-L-arafase II
alpha-L-arafase I


organism: Streptomyces purpurascens


organism: Streptomyces sp.


organism: Penicillium oxalicum


organism: Penicillium subrubescens

proof_lines:
isoform AbfF, pH 5, 30°C
isoform AbfA, pH 5, 30°C
isoform AbfC, pH 5, 30°C


organism: Rhodotorula flava


organism: Ruminococcus albus


organism: Streptomyces sp. PC22

assay_conditions_summary: pH 6


organism: Talaromyces amestolkiae

proof_lines:
isoform Ara2, pH 5, 50°C
isoform Ara1, pH 5, 50°C


organism: Talaromyces purpureogenus

substrate_name: 4-nitrophenyl beta-D-galactofuranoside

substrate: 4-nitrophenyl beta-D-galactofuranoside


organism: Thermobacillus xylanilyticus

substrate_name: p-nitrophenyl-alpha-L-arabinose

substrate: p-nitrophenyl-alpha-L-arabinose


organism: Thermobifida fusca

assay_conditions_summary: pH 9


organism: Thermomyces dupontii

assay_conditions_summary: pH 7; 323.15 K; buffer 50 mM potassium phosphate

proof_lines: in 50 mM potassium phosphate buffer (pH 7.0), at 50°C


organism: Trametes hirsuta

substrate_name: arabinoxylan

substrate: arabinoxylan


organism: Trichoderma sp.


organism: Geobacillus stearothermophilus


organism: Glycine max

substrate_name: 4-nitrophenyl alpha-L-arabinofuranoside

substrate: 4-nitrophenyl alpha-L-arabinofuranoside


organism: Hordeum vulgare


organism: Levilactobacillus brevis


organism: Bussuquara virus


organism: Dengue virus

proof_lines: pH 8.5, 37°C, enzyme from Dengue virus serotype 1

proof_lines: pH 8.5, 37°C, enzyme from Dengue virus serotype 3

proof_lines: pH 8.5, 37°C, enzyme from Dengue virus serotype 4


organism: dengue virus type 2

substrate_name: Gly-Arg-Arg-4-methylcoumarin 7-amide

proof_lines: pH 9.0, 37°C, wild-type NS3 protease with wild-tyüe NS2B cofactor

substrate: Gly-Arg-Arg-4-methylcoumarin 7-amide


proof_lines: predicted inhibition constant, pH and temperature not specified in the publication


organism: Zika virus

proof_lines: pH and temperature not specified in the publication


organism: Murray Valley encephalitis virus

substrate_name: Unknown

substrate: Unknown


organism: St. Louis encephalitis virus


organism: West Nile virus


organism: Yellow fever virus


organism: Avena sativa

substrate_name: biliverdin IXa

proof_lines:
above
Porphyra umbilicalis ferredoxin
Spinacia oleracea ferredoxin

substrate: biliverdin IXa


organism: Aspergillus flavipes

substrate_name: glutathione

substrate: glutathione


enzyme_display_name: NADPH--cytochrome-c2 reductase

organism: Cereibacter sphaeroides

enzyme: NADPH--cytochrome-c2 reductase


organism: Hapsidospora chrysogena

substrate_name: acetyl-CoA

assay_conditions_summary: cofactor CoA

substrate: acetyl-CoA


organism: Homo sapiens

substrate_name: lyso-phosphatidylserine

substrate: lyso-phosphatidylserine


organism: Oryctolagus cuniculus


organism: Rattus norvegicus


organism: Kluyveromyces lactis

substrate_name: UDP-N-acetylglucosamine

substrate: UDP-N-acetylglucosamine


proof_lines:
solubilized enzyme
membrane-bound enzyme


enzyme_display_name: monoterpenol beta-glucosyltransferase

organism: Mentha x piperita

substrate_name: UDPglucose

proof_lines:
reaction with D-neomenthol
reaction with L-menthol

enzyme: monoterpenol beta-glucosyltransferase

substrate: UDPglucose


substrate_name: GDPmannose

proof_lines: based on the assumption of incorporation of only a single mannosyl unit into the product

substrate: GDPmannose


organism: Sus scrofa


enzyme_display_name: nicotinate glucosyltransferase

organism: Petroselinum crispum

substrate_name: nicotinic acid

enzyme: nicotinate glucosyltransferase

substrate: nicotinic acid


enzyme_display_name: soyasapogenol glucuronosyltransferase

organism: Glycine max

substrate_name: soyasapogenol A

proof_lines: using soyasapogenol B as the sugar acceptor, in 100 mM Tris-HCl (pH 8.0), at 40°C

enzyme: soyasapogenol glucuronosyltransferase

substrate: soyasapogenol A


organism: Arabidopsis thaliana

substrate_name: UDP-xylose

proof_lines: pH 7.0, 37°C, recombinant mutant DELTA39 XylT

substrate: UDP-xylose


enzyme_display_name: diiodotyrosine transaminase

enzyme: diiodotyrosine transaminase


enzyme_display_name: uracilylalanine synthase

organism: Lathyrus oleraceus

substrate_name: Uracil

proof_lines:
willardiine synthesis
synthesis of isowillardiine

enzyme: uracilylalanine synthase

substrate: Uracil


enzyme_display_name: 4-hydroxyglutamate transaminase

enzyme: 4-hydroxyglutamate transaminase


enzyme_display_name: ADP--thymidine kinase

organism: Herpes simplex virus

substrate_name: thymidine

assay_conditions_summary: cofactor ADP

proof_lines:
Herpes simplex virus type 2
Herpes simplex virus type 1

enzyme: ADP--thymidine kinase

substrate: thymidine


enzyme_display_name: diphosphate--serine phosphotransferase

organism: Propionibacterium freudenreichii subsp. shermanii

substrate_name: diphosphate

proof_lines:
pH 7.8, 37°C
1 mM diphosphate, pH 7.8, 37°C

enzyme: diphosphate--serine phosphotransferase

substrate: diphosphate


enzyme_display_name: [heparan sulfate]-glucosamine 3-sulfotransferase 1

organism: Mus musculus

substrate_name: 3'-phosphoadenosine 5'-phosphosulfate

substrate: 3'-phosphoadenosine 5'-phosphosulfate


enzyme: [heparan sulfate]-glucosamine 3-sulfotransferase 1


organism: Zika virus

proof_lines: pH 8.5, 37°C, Tris-CHAPS-glycerol buffer

proof_lines: pH 8.5, temperature not specified in the publication


organism: Thermococcus kodakarensis

substrate_name: adenine9 in tRNAThr

proof_lines: pH 8.0, 50°C, recombinant enzyme, tRNA substrate from Thermococcus kodakarensis

proof_lines: pH 8.0, 40°C, recombinant enzyme, tRNA substrate from Saccharomyces cerevisiae

substrate: adenine9 in tRNAThr


organism: Bacteroides fragilis

substrate_name: N-succinyl-L-ornithine

substrate: N-succinyl-L-ornithine


enzyme_display_name: apulose-4-phosphate transketolase

organism: Actinobacillus succinogenes

substrate_name: D-Apulose 4-phosphate; D-Glyceraldehyde 3-phosphate

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

enzyme: apulose-4-phosphate transketolase


enzyme_display_name: acyl-Kdo2-lipid IVA acyltransferase

organism: Acinetobacter baumannii

substrate_name: hexadecanoyl-[acyl-carrier protein]

enzyme: acyl-Kdo2-lipid IVA acyltransferase

substrate: hexadecanoyl-[acyl-carrier protein]


organism: Oryza sativa

proof_lines: recombinant soluble enzyme, pH 6.0, 16°C


organism: Aspergillus fumigatus

substrate_name: dimethylallyl diphosphate

proof_lines: 37°C, pH not specified in the publication

substrate: dimethylallyl diphosphate


enzyme_display_name: O-phosphoseryl-tRNA(Sec) kinase

organism: Methanocaldococcus jannaschii

substrate_name: ATP

assay_conditions_summary: 310.15 K; cofactor ATP

enzyme: O-phosphoseryl-tRNA(Sec) kinase

substrate: ATP


enzyme_display_name: flavivirin

organism: West Nile virus


enzyme_display_name: catalase peroxidase

organism: Mycobacterium tuberculosis


proof_lines: mutant enzyme I290V/Q293V, at pH 5.0 and 23°C


enzyme_display_name: quercetin-3-sulfate 3'-sulfotransferase

organism: Flaveria chlorifolia

enzyme: quercetin-3-sulfate 3'-sulfotransferase


organism: Chloroflexus aurantiacus

substrate_name: succinyl-CoA

assay_conditions_summary: 328.15 K; cofactor CoA

proof_lines: 55°C, pH and temperature not specified in the publication

substrate: succinyl-CoA


enzyme_display_name: ellagitannin acyl hydrolase

organism: Aspergillus niger

substrate_name: punicalagin

enzyme: ellagitannin acyl hydrolase

substrate: punicalagin


enzyme_display_name: 3-oxoadipate enol-lactonase

organism: Acinetobacter calcoaceticus

substrate_name: Unknown

enzyme: 3-oxoadipate enol-lactonase

substrate: Unknown


organism: Pseudomonas putida


enzyme_display_name: wax-ester hydrolase

organism: Simmondsia chinensis

substrate_name: N-methylindoxyl myristate

enzyme: wax-ester hydrolase

substrate: N-methylindoxyl myristate


organism: Sinapis alba

substrate_name: cholesteryl palmitate

substrate: cholesteryl palmitate


enzyme_display_name: 3-phosphoglycerate phosphatase

organism: Saccharum sp.

substrate_name: D-glycerate 3-phosphate

enzyme: 3-phosphoglycerate phosphatase

substrate: D-glycerate 3-phosphate


organism: Spinacia oleracea


enzyme_display_name: caldesmon-phosphatase

organism: Meleagris gallopavo

substrate_name: calponin

proof_lines: pH 7.5, 30°C, calponin phosphorylated by PKC

proof_lines: pH 7.5, 30°C, caldesmon phosphorylated by PKC cdc2

proof_lines: pH 7.5, 30°C, caldesmon phosphorylated by PKC CaM kinase II

enzyme: caldesmon-phosphatase

substrate: calponin


enzyme_display_name: D-arabinan exo alpha-(1,3)/(1,5)-arabinofuranosidase (non-reducing end)

organism: Dysgonomonas gadei

substrate_name: 4-nitrophenyl alpha-D-arabinofuranoside

substrate: 4-nitrophenyl alpha-D-arabinofuranoside


organism: Microbacterium arabinogalactanolyticum

enzyme: D-arabinan exo alpha-(1,3)/(1,5)-arabinofuranosidase (non-reducing end)


organism: Nocardia brasiliensis


enzyme_display_name: peptidyl-glycinamidase

organism: Rattus norvegicus

enzyme: peptidyl-glycinamidase


enzyme_display_name: oviductin

organism: Xenopus laevis

enzyme: oviductin


enzyme_display_name: DNA-3-methyladenine glycosylase I

organism: Escherichia coli

substrate_name: 3-methyladenine residues in alkylated DNA

proof_lines: pH 7.6, 37°C, release of 3-methyladenine

proof_lines: pH 7.8, 37°C, release of 3-methyladenine

enzyme: DNA-3-methyladenine glycosylase I


enzyme_display_name: licheninase

organism: Bacillus subtilis

substrate_name: H2O; Lichenin

enzyme: licheninase

substrate: H2O; Lichenin


organism: Streptococcus bovis

substrate_name: Lichenan; H2O

substrate: Lichenan; H2O


organism: Cupriavidus necator

substrate_name: 3-oxo-tetronate 4-phosphate

proof_lines: pH and temperature not specified in the publication

substrate: 3-oxo-tetronate 4-phosphate


organism: Haemophilus influenzae


organism: Pectobacterium atrosepticum


organism: Sulfolobus solfataricus

substrate_name: Pyruvate; D-Glyceraldehyde

substrate: Pyruvate; D-Glyceraldehyde


enzyme_display_name: xylose isomerase

organism: Actinoplanes missouriensis

substrate_name: D-glucose

enzyme: xylose isomerase

substrate: D-glucose


source_db: skid

source_license: CC BY-NC-ND 4.0

source: skid


organism: Arthrobacter sp.

substrate_name: D-Xylose

substrate: D-Xylose


substrate_name: NAD+

assay_conditions_summary: 303.15 K; cofactor NADP+

proof_lines: cofactor NAD+, pH not specified in the publication, 30°C

proof_lines: cofactor NADP+, pH not specified in the publication, 30°C

substrate: NAD+


organism: Crocosphaera subtropica

substrate_name: NADP+

assay_conditions_summary: pH 9; 298.15 K; cofactor NADP+

proof_lines: pH 9.0, 25°C, recombinant wild-type enzyme, with NADP+

proof_lines: pH 9.0, 25°C, recombinant wild-type enzyme, with NAD+

substrate: NADP+


proof_lines: pH 9.0, 25°C, recombinant mutant S157E, with NADP+

proof_lines: pH 9.0, 25°C, recombinant mutant S157E, with NAD+


proof_lines: pH 9.0, 25°C, recombinant mutant K154A, with NADP+

proof_lines: pH 9.0, 25°C, recombinant mutant K154A, with NAD+


organism: Nitrosomonas europaea

assay_conditions_summary: pH 8.5; 303.15 K; cofactor NADP+

proof_lines: pH 8.5, 30°C, recombinant enzyme, with succinate semialdehyde

proof_lines: pH 8.5, 30°C, recombinant enzyme, with NADP+

proof_lines: pH 8.5, 30°C, recombinant enzyme, with NAD+


organism: Salmonella enterica subsp. enterica serovar Typhimurium

proof_lines: mutant enzyme K160E, in 50 mM HEPES (pH 8.0), at 37°C


substrate_name: succinate semialdehyde

assay_conditions_summary: pH 8; 310.15 K; cofactor NAD+

proof_lines: mutant enzyme F138A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C

substrate: succinate semialdehyde


proof_lines: mutant enzyme W141A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C


proof_lines: mutant enzyme K160A, in 50 mM HEPES (pH 8.0), at 37°C


assay_conditions_summary: pH 8; 310.15 K; cofactor NADP+

proof_lines: wild type enzyme, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C

proof_lines: wild type enzyme, in 50 mM HEPES (pH 8.0), at 37°C

proof_lines: wild type enzyme, with NADP+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C


organism: Saccharolobus solfataricus

substrate_name: KDPG (at 60 degrees Celsius and at pH 6)

source_db: uniprot

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: kcat is 28.2 sec(-1) for KDG and 6.8 sec(-1) for KDGal.

source: uniprot


organism: Cereibacter sphaeroides

assay_conditions_summary: cofactor NADP+


assay_conditions_summary: pH 6.5; 343.15 K; cofactor NADP+


organism: Euglena gracilis


organism: Klebsiella pneumoniae

assay_conditions_summary: pH 7.8; 303.15 K; cofactor NADP+


organism: Pseudomonas putida

assay_conditions_summary: pH 8.5; 303.15 K; buffer TAPS; cofactor NADP+

proof_lines: recombinant wild type enzyme, in TAPS buffer, at pH 8.5, at 30°C

proof_lines: native wild type enzyme, in TAPS buffer, at pH 8.5, at 30°C


assay_conditions_summary: pH 8; 303.15 K; cofactor NADP+

proof_lines: His-tagged BADH, cosubstrate benzaldehyde


organism: Mycobacterium tuberculosis

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NADP+

proof_lines: in the presence of 10 mM Mg2+, at pH 7.5 and 37°C

proof_lines: with NADP+ as cosubstrate, in the absence of Mg2+, at pH 7.5 and 37°C


organism: Rattus norvegicus

assay_conditions_summary: cofactor NAD+

proof_lines:
synaptosomes
mitochondria of non-synaptic origin


substrate_name: 2-oxoglutarate semialdehyde

proof_lines: with NAD+ as cosubstrate, at pH 6.5 and 70°C

proof_lines: with NADP+ as cosubstrate, at pH 6.5 and 70°C

substrate: 2-oxoglutarate semialdehyde


organism: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)

substrate_name: pyruvate (at pH 6 and at 70 degrees Celsius)

assay_conditions_summary: pH 6


organism: Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)

substrate: pyruvate (at pH 6 and at 70 degrees Celsius)


enzyme_display_name: N-benzyloxycarbonylglycine hydrolase

organism: Enterococcus faecalis

substrate_name: N-benzyl-Gly

enzyme: N-benzyloxycarbonylglycine hydrolase

substrate: N-benzyl-Gly


organism: Stutzerimonas stutzeri

assay_conditions_summary: pH 8.5; 298.15 K; cofactor NADP+

proof_lines: in the presence of benzaldehyde, at 25°C and pH 8.5

proof_lines: in the presence of NAD+, at 25°C and pH 8.5

proof_lines: in the presence of NADP+, at 25°C and pH 8.5


enzyme_display_name: CDP-diacylglycerol diphosphatase

organism: Cavia porcellus

substrate_name: CDPdipalmitoylglycerol

enzyme: CDP-diacylglycerol diphosphatase

substrate: CDPdipalmitoylglycerol


enzyme_display_name: bromoxynil nitrilase

organism: Klebsiella pneumoniae subsp. ozaenae

enzyme: bromoxynil nitrilase


organism: Piromyces sp.

substrate_name: D-xylose

assay_conditions_summary: pH 7.5; 303.15 K; buffer 100 mM Tris-HCl

proof_lines: mutant enzyme E129D/V433I/E15D/E114G/T142S/A177T, in 100 mM Tris-HCl buffer at pH 7.5 and 30°C

substrate: D-xylose


enzyme_display_name: N-carbamoylsarcosine amidase

organism: Arthrobacter sp.

substrate_name: N-carbamoylsarcosine

enzyme: N-carbamoylsarcosine amidase

substrate: N-carbamoylsarcosine


organism: Clostridium sp.


enzyme_display_name: D-lyxose ketol-isomerase

organism: Thermofilum sp. ex4484_79

substrate_name: D-Lyxose

enzyme: D-lyxose ketol-isomerase

substrate: D-Lyxose


organism: Thermoflavimicrobium dichotomicum

substrate_name: L-ribose

substrate: L-ribose


organism: Thermoprotei archaeon


organism: Thermosediminibacter oceani

substrate_name: D-fructose

substrate: D-fructose


enzyme_display_name: catalase peroxidase

organism: Archaeoglobus fulgidus

proof_lines: peroxidase activity, 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C

enzyme: catalase peroxidase


organism: Bacillus pumilus


organism: Burkholderia pseudomallei

proof_lines: mutant enzyme R108A/D141A, peroxidase activity, at pH 4.5 and 25°C

proof_lines: mutant enzyme R108A/D141A, catalase activity, at pH 7.0 and 37°C


proof_lines: mutant enzyme D141A, peroxidase activity, at pH 4.5 and 25°C

proof_lines: mutant enzyme D141A, catalase activity, at pH 7.0 and 37°C


proof_lines: mutant enzyme R108A, catalase activity, at pH 7.0 and 37°C

proof_lines: mutant enzyme R108A, peroxidase activity, at pH 4.5 and 25°C


proof_lines: mutant enzyme D141E, peroxidase activity, at pH 4.5 and 25°C

proof_lines: mutant enzyme D141E, catalase activity, at pH 7.0 and 37°C


proof_lines: mutant enzyme R108A/W111F/D141A, catalase activity, at pH 7.0 and 37°C

proof_lines: mutant enzyme R108A/W111F/D141A, peroxidase activity, at pH 4.5 and 25°C


proof_lines: mutant enzyme D141N, peroxidase activity, at pH 4.5 and 25°C

proof_lines: mutant enzyme D141N, catalase activity, at pH 7.0 and 37°C


proof_lines: mutant enzyme R108A/W111F, catalase activity, at pH 7.0 and 37°C

proof_lines: mutant enzyme R108A/W111F, peroxidase activity, at pH 4.5 and 25°C


proof_lines: wild type enzyme, peroxidase activity, at pH 4.5 and 25°C

proof_lines: wild type enzyme, catalase activity, at pH 7.0 and 37°C


proof_lines: mutant enzyme R108K, peroxidase activity, at pH 4.5 and 25°C

proof_lines: mutant enzyme R108K, catalase activity, at pH 7.0 and 37°C


organism: Mycobacterium tuberculosis

substrate_name: o-Dianisidine

proof_lines:
mutant enzyme N238S, at pH 4.5 and 30°C
mutant enzyme N238S, at pH 7.0 and 30°C

substrate: o-Dianisidine


enzyme_display_name: toluene 4-monooxygenase

organism: Burkholderia cepacia

substrate_name: methyl 4-tolyl sulfide

proof_lines: whole-cell assay, 25°C, pH not specified in the publication

enzyme: toluene 4-monooxygenase

substrate: methyl 4-tolyl sulfide


organism: Ectopseudomonas mendocina

substrate_name: Toluene

substrate: Toluene


proof_lines:
wild-type, pH 7.5, 25°C
wild-type, pH 7.5, 4°C


substrate_name: norcarane

proof_lines:
mutant T201A, pH 7.5, 25°C
mutant T201A, pH 7.5, 4°C

substrate: norcarane


enzyme_display_name: dichloromethane dehalogenase

organism: Methylophilus sp.

substrate_name: Dichloromethane

enzyme: dichloromethane dehalogenase

substrate: Dichloromethane


organism: Methylorubrum extorquens


organism: Methylorubrum rhodesianum


proof_lines: pH 8.0, 30°C, recombinant wild-type enzyme


organism: Niallia circulans


enzyme_display_name: L-Ala-D/L-Glu epimerase

organism: Bacillus subtilis

substrate_name: L-Alanyl-D-aspartate

source_db: oed

enzyme: L-Ala-D/L-Glu epimerase

substrate: L-Alanyl-D-aspartate

source: oed


substrate_name: L-Alanyl-D-glutamate

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

proof_lines:
pH 8.5, 37°C
Raw SKiD Env: Temp=37°C, pH=8.5

source_databases_merged: sabio_rk brenda skid

substrate: L-Alanyl-D-glutamate


substrate_name: L-Ala-D-Asp

substrate: L-Ala-D-Asp


enzyme_display_name: alpha,alpha-trehalose synthase

organism: Rubrobacter xylanophilus (strain DSM 9941 / JCM 11954 / NBRC 16129 / PRD-1)

substrate_name: ADP-glucose

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

assay_conditions_summary: cofactor ADP

enzyme: alpha,alpha-trehalose synthase

substrate: ADP-glucose


organism: Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C)


enzyme_display_name: fucokinase

organism: Arabidopsis thaliana

substrate_name: L-fucose for the L-fucokinase activity

assay_conditions_summary: cofactor ATP

enzyme: fucokinase

substrate: L-fucose for the L-fucokinase activity


enzyme_display_name: transaldolase

organism: Unknown

substrate_name: D-fructose 6-phosphate

enzyme: transaldolase

substrate: D-fructose 6-phosphate


enzyme_display_name: N-acylglucosamine 2-epimerase

organism: Anabaena sp. CH1

substrate_name: N-acetyl-D-glucosamine

proof_lines: E242Q, reverse conversion in the presence of 1 mM ATP

enzyme: N-acylglucosamine 2-epimerase

substrate: N-acetyl-D-glucosamine


substrate_name: N-acetyl-D-mannosamine

proof_lines: C370A, reverse conversion in the presence of 1 mM ATP

substrate: N-acetyl-D-mannosamine


proof_lines: R375K, reverse conversion in the presence of 1 mM ATP


proof_lines: E218D, reverse conversion in the presence of 1 mM ATP


proof_lines: E218A, reverse conversion in the presence of 1 mM ATP


proof_lines: R375A, reverse conversion in the presence of 1 mM ATP


organism: Homo sapiens

substrate_name: N-Acetyl-D-glucosamine

substrate: N-Acetyl-D-glucosamine


organism: Sus scrofa


organism: Trichormus variabilis

proof_lines: pH 7.5, 30°C, recombinant His6-tagged wild-type enzyme


proof_lines: pH 7.5, 30°C, recombinant His6-tagged mutant K160P


proof_lines: pH 7.5, 30°C, recombinant His6-tagged mutant K155A


organism: Bacillus methanolicus

sequence_source: UniProtKB accession metadata


organism: Blastobotrys adeninivorans


organism: Escherichia coli

proof_lines: mutant F189Y, reaction: sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate formation from erythrose 4-phosphate + D-fructose 6-phosphate

proof_lines: mutant F189Y, reaction: fructose 6-phosphate synthesis from dihydroxyacetone and glyceraldehyde 3-phosphate

proof_lines: mutant F189Y, reaction: fructose 6-phosphate cleavage into dihydroxyacetone and glyceraldehyde 3-phosphate


organism: Thermoplasma acidophilum


substrate_name: D-Fructose 6-phosphate

substrate: D-Fructose 6-phosphate


proof_lines: mutant enzyme E60Q/F132Y, at pH 7.5 and 30°C


organism: Thermotoga maritima

source_license: CC BY 4.0; CC BY-NC-ND 4.0

proof_lines:
pH 9.0, 80°C
Raw SKiD Env: Temp=80°C, pH=9

source_databases_merged: brenda skid


organism: Pyrococcus horikoshii

substrate_name: D-glucose

substrate: D-glucose


organism: Rubrobacter xylanophilus

assay_conditions_summary: 333.15 K; cofactor ADP


organism: Bacteroides fragilis

substrate_name: L-fucose

proof_lines: pH and temperature not specified in the publication

substrate: L-fucose


substrate_name: D-erythrose 4-phosphate

substrate: D-erythrose 4-phosphate


proof_lines: kcat is 0.39 sec(-1) for the fucokinase activity. kcat is 10.07 sec(-1) for the fucose-1-phosphate guanylyltransferase activity.


enzyme_display_name: stizolobate synthase

organism: Beta vulgaris

substrate_name: L-dopa

enzyme: stizolobate synthase

substrate: L-dopa


organism: Escherichia coli (strain K12)

substrate_name: L-DOPA

substrate: L-DOPA


enzyme_display_name: [protein]-arginine 3-hydroxylase

substrate_name: Arg-137 of RPS6

enzyme: [protein]-arginine 3-hydroxylase

substrate: Arg-137 of RPS6


organism: Streptomyces globisporus


organism: Xenorhabdus nematophila (strain ATCC 19061 / DSM 3370 / CCUG 14189 / LMG 1036 / NCIMB 9965 / AN6)

substrate_name: tyrosine isonitrile

substrate: tyrosine isonitrile


enzyme_display_name: levoglucosan dehydrogenase

organism: Pseudarthrobacter phenanthrenivorans (strain DSM 18606 / JCM 16027 / LMG 23796 / Sphe3)

substrate_name: levoglucosan (at pH 8.5 and 40 degrees Celsius)

proof_lines: kcat is 4.3 sec(-1) with levoglucosan as substrate. kcat is 26 sec(-1) with 3-dehydrolevoglucosan as substrate.

enzyme: levoglucosan dehydrogenase


enzyme_display_name: sulfide dehydrogenase

organism: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

substrate_name: NADPH (with benzyl viologen as cosubstrate at pH 9.5)

enzyme: sulfide dehydrogenase


substrate: NADPH (with benzyl viologen as cosubstrate at pH 9.5)


substrate_name: 16S rRNA

proof_lines: kcat is 0.012 sec(-1) with 16S rRNA as substrate.

substrate: 16S rRNA


organism: Streptomyces niveus

substrate_name: demethyldecarbamoylnovobiocin

proof_lines: kcat is 0.4 min(-1) with demethyldecarbamoylnovobiocin as substrate.

substrate: demethyldecarbamoylnovobiocin


substrate_name: D,L-glyceraldehyde 3-phosphate (with C3 acceptor compounds at pH 8.5 and 30 degrees Celsius)

proof_lines: kcat is 53 sec(-1) for D-fructose-6-phosphate.


organism: Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936)

proof_lines: kcat is 4114 min(-1) with D-erythrose 4-phosphate as substrate and 4151 min(-1) with D-fructose 6-phosphate as substrate.


enzyme_display_name: licheninase

organism: Bacillus amyloliquefaciens

substrate_name: H2O; 1,3-beta-D-Glucan

unit: l/(min*g)

enzyme: licheninase

substrate: H2O; 1,3-beta-D-Glucan


source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

proof_lines: fusion protein encoding beta-1,3-1,4-glucanase both from Bacillus amyloliquefaciens and Clostridium thermocellum, pH 6.0, 70°C


organism: Bacillus licheniformis


enzyme_display_name: dipeptidyl-peptidase I

organism: Homo sapiens

substrate_name: Gly-Phe-4-methoxynaphthylamide; H2O

enzyme: dipeptidyl-peptidase I

substrate: Gly-Phe-4-methoxynaphthylamide; H2O


organism: Rattus norvegicus

substrate_name: Glycyl-Tyrosyl-amide; Hydroxylamine

substrate: Glycyl-Tyrosyl-amide; Hydroxylamine


organism: Methylophilus sp.


substrate_name: Dihalomethane; H2O

substrate: Dihalomethane; H2O


substrate_name: Dichloromethane

substrate: Dichloromethane


substrate_name: Dibromomethane

substrate: Dibromomethane


proof_lines:
30°C, pH 8.5, wild-type
pH 8.5, wild-type
pH 8.2, wild-type


organism: Ricinus communis

substrate_name: hexadecanoyl-[acyl-carrier protein]

proof_lines: wild type enzyme, pH and temperature not specified in the publication

substrate: hexadecanoyl-[acyl-carrier protein]


substrate_name: octadecanoyl-[acyl-carrier protein]

proof_lines: mutant enzyme T117R/G188L, pH and temperature not specified in the publication

substrate: octadecanoyl-[acyl-carrier protein]


substrate_name: Reduced ferredoxin; Stearoyl-[acyl-carrier protein]; O2


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: succinate semialdehyde

proof_lines: mutant enzyme D426A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C

substrate: succinate semialdehyde


proof_lines: mutant enzyme R428A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C


substrate_name: NAD+

proof_lines: mutant enzyme N193A, in 50 mM HEPES (pH 8.0), at 37°C

substrate: NAD+


proof_lines: mutant enzyme S425A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C


proof_lines: mutant enzyme R145A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C


proof_lines: mutant enzyme K92A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C


proof_lines: mutant enzyme E442A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C


organism: Streptococcus pyogenes

substrate_name: NADP+

assay_conditions_summary: pH 7; 303.15 K; cofactor NADP+

proof_lines: recombinant His6-tagged enzyme, pH 7.0, 30°C

substrate: NADP+


organism: Sulfurisphaera tokodaii

assay_conditions_summary: pH 8; 313.15 K; cofactor NADP+

proof_lines:
pH 8.0, 40°C, cosubstrate: NADP+
pH 8.0, 60°C
pH 8.0, 40°C, cosubstrate: NAD+


enzyme_display_name: 2-oxoglutarate synthase

organism: Hydrogenobacter thermophilus

substrate_name: CoA

assay_conditions_summary: pH 7.8; 343.15 K; cofactor CoA

enzyme: 2-oxoglutarate synthase

substrate: CoA


proof_lines: isozyme Kor, in 10 mM MOPS, pH 6.8, at 70°C


substrate_name: reduced ferredoxin

proof_lines: isozyme For, in 10 mM MOPS, pH 6.8, at 70°C

substrate: reduced ferredoxin


organism: Magnetococcus marinus

substrate_name: succinyl-CoA

assay_conditions_summary: pH 7; cofactor CoA

proof_lines: wild-type, pH 7.0, temperature not specified in the publication

proof_lines: wild-type, pH 8.5, temperature not specified in the publication

proof_lines: wild-type, pH 7.5, temperature not specified in the publication

substrate: succinyl-CoA


proof_lines: mutant Y436F, pH 7.5, temperature not specified in the publication


proof_lines: mutant E45Q, pH 7.5, temperature not specified in the publication


organism: Thauera aromatica

assay_conditions_summary: pH 7.8; 303.15 K; cofactor CoA


enzyme_display_name: 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH)

organism: Chlorobaculum tepidum

substrate_name: 3,8-divinyl chlorophyllide a

proof_lines: recombinant BciA, pH and temperature not specified in the publication

substrate: 3,8-divinyl chlorophyllide a


organism: Rhodobacter capsulatus

proof_lines: pH 8.0, 22°C, recombinant Strep II-tagged oligomeric enzyme, low salt condition

proof_lines: pH 8.0, 22°C, recombinant Strep II-tagged dimeric enzyme, low salt condition

proof_lines: pH 8.0, 22°C, recombinant Strep II-tagged dimeric enzyme, high salt condition

enzyme: 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH)


enzyme_display_name: L-alanine--L-anticapsin ligase

organism: Bacillus subtilis

substrate_name: L-alanine

enzyme: L-alanine--L-anticapsin ligase

substrate: L-alanine


proof_lines:
pH 7.7, 37°C, mutant enzyme Y75F
pH 7.1, 37°C, mutant enzyme Y75F


proof_lines:
pH 7.7, 37°C, wild-type enzyme
pH 7.1, 37°C, wild-type enzyme


substrate_name: L-Alanyl-D-methionine

substrate: L-Alanyl-D-methionine


substrate_name: L-Ala-D-Glu

substrate: L-Ala-D-Glu


substrate_name: L-Ala-D-Met

substrate: L-Ala-D-Met


organism: Escherichia coli


substrate_name: L-Ala-D-Gln

substrate: L-Ala-D-Gln


substrate_name: L-Ala-D-Asp

substrate: L-Ala-D-Asp


organism: Anabaena sp. CH1

substrate_name: N-acetyl-D-mannosamine

proof_lines: N179A, reverse conversion in the presence of 1 mM ATP

substrate: N-acetyl-D-mannosamine


assay_conditions_summary: pH 7; cofactor ATP

proof_lines: wild type, at pH 7, in the presence of 1 mM ATP

proof_lines: wild type, at pH 8, in the presence of 1 mM ATP

proof_lines: wild type, at pH 9, in the presence of 1 mM ATP


organism: Trichormus variabilis

substrate_name: N-acetyl-D-glucosamine

proof_lines: pH 7.5, 30°C, recombinant His6-tagged mutant K151V

substrate: N-acetyl-D-glucosamine


proof_lines: pH 7.5, 30°C, recombinant His6-tagged mutant K157L


enzyme_display_name: D-lyxose ketol-isomerase

organism: Bacillus licheniformis

substrate_name: D-mannose

proof_lines: in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C

enzyme: D-lyxose ketol-isomerase

substrate: D-mannose


organism: Bacillus velezensis

substrate_name: D-Lyxose

substrate: D-Lyxose


organism: Cohnella laeviribosi


organism: Dictyoglomus turgidum

substrate_name: L-ribulose

proof_lines:
at pH 7.5 and 75°C
pH 7.5, 75°C

substrate: L-ribulose


organism: Providencia stuartii

substrate_name: D-xylulose

substrate: D-xylulose


organism: Serratia proteamaculans

proof_lines: at pH 7.5 and 40°C, in the presence of 1 mM Mn2+


substrate_name: ATP; L-Alanine; L-Glutamine

assay_conditions_summary: pH 9; 310.15 K; cofactor ATP

proof_lines:
pH 9.0, 37°C
pH 7.7, 37°C
Raw SKiD Env: Temp=37°C, pH=7.1

substrate: ATP; L-Alanine; L-Glutamine


substrate_name: L-phenylalanine

proof_lines:
pH 7.7, 37°C, mutant enzyme
pH 7.1, 37°C, mutant enzyme

substrate: L-phenylalanine


enzyme_display_name: catalase peroxidase

organism: Mycobacterium tuberculosis

enzyme: catalase peroxidase


proof_lines: mutant enzyme I290A/Q293A, at pH 5.0 and 23°C


enzyme_display_name: flavivirin

organism: Alkhumra hemorrhagic fever virus

enzyme: flavivirin


substrate_name: D-fructose 6-phosphate

substrate: D-fructose 6-phosphate


substrate_name: glyceraldehyde 3-phosphate

proof_lines:
wild-type, pH 8.5, 30°C
wild-type
wild-type, N35A

substrate: glyceraldehyde 3-phosphate


substrate_name: D-glyceraldehyde

substrate: D-glyceraldehyde


substrate_name: 3,8-divinyl protochlorophyllide a

proof_lines: oligomeric enzyme (low salt condition, 150 mM NaCl), at pH 8.0 and 25°C

proof_lines: pH and temperature not specified in the publication

proof_lines: dimeric enzyme (low salt condition, 150 mM NaCl), at pH 8.0 and 25°C

substrate: 3,8-divinyl protochlorophyllide a


organism: Hydrogenobacter thermophilus

assay_conditions_summary: pH 7.6; 343.15 K; cofactor CoA


organism: Prosthecochloris vibrioformis f. thiosulfatophilum

substrate_name: succinyl-CoA

assay_conditions_summary: cofactor CoA

substrate: succinyl-CoA


organism: Thermococcus litoralis

substrate_name: oxidized ferredoxin

assay_conditions_summary: pH 8.4; 353.15 K; cofactor CoA

proof_lines: pH 8.4, 80°C, ferredoxin from Pyrococcus furiosus

substrate: oxidized ferredoxin


organism: Arthrobacter sp.


organism: Blastobotrys adeninivorans


organism: Bos taurus

substrate_name: D-erythrose 4-phosphate

substrate: D-erythrose 4-phosphate


organism: Cyberlindnera jadinii


organism: Dictyostelium discoideum


organism: Escherichia coli

proof_lines: transaldolase B, competitive inhibition with respect to D-erythrose 4-phosphate, at pH 7.2 and 37°C


organism: Fusarium oxysporum


organism: Homo sapiens

proof_lines: mutant F189Y, reaction: fructose 6-phosphate cleavage into dihydroxyacetone and glyceraldehyde 3-phosphate

proof_lines: mutant F189Y, reaction: sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate formation from erythrose 4-phosphate + D-fructose 6-phosphate

proof_lines: mutant F189Y, reaction: fructose 6-phosphate synthesis from dihydroxyacetone and glyceraldehyde 3-phosphate


organism: Pyrococcus horikoshii

substrate_name: UDP

assay_conditions_summary: pH 6; 333.15 K; cofactor ADP

substrate: UDP


assay_conditions_summary: pH 6.5; 363.15 K; cofactor ADP


organism: Thermoproteus tenax

substrate_name: UDP-glucose

proof_lines: in 50 mM HEPES/KOH (pH 7.0), with 20 mM MgCl2, at 70°C

substrate: UDP-glucose


organism: Thermotoga maritima

substrate_name: GDP-alpha-D-glucose

substrate: GDP-alpha-D-glucose


organism: Methanocaldococcus jannaschii

substrate_name: D-ribose 5-phosphate

proof_lines: pH 7.5, 50°C, concentration of ribose-5-phosphate between 0.5 and 15 mM

substrate: D-ribose 5-phosphate


organism: Saccharomyces cerevisiae

substrate_name: sedoheptulose 7-phosphate

substrate: sedoheptulose 7-phosphate


organism: Solanum lycopersicum

substrate_name: erythrose 4-phosphate

substrate: erythrose 4-phosphate


organism: Rattus norvegicus

substrate_name: Unknown

substrate: Unknown


organism: Vitis vinifera

substrate_name: ATP

substrate: ATP


organism: Arabidopsis thaliana

substrate_name: L-fucose

assay_conditions_summary: pH 7.5; 308.15 K; cofactor ATP

substrate: L-fucose


organism: Bacteroides fragilis

substrate_name: L-galactose

substrate: L-galactose


organism: Canis lupus familiaris

assay_conditions_summary: pH 6.5; 310.15 K; cofactor ATP


organism: Mortierella alpina


enzyme_display_name: dichloromethane dehalogenase

organism: Niallia circulans

substrate_name: Dichloromethane

enzyme: dichloromethane dehalogenase

substrate: Dichloromethane


organism: Hyphomicrobium sp.


organism: Hyphomicrobium sp. DM2


organism: Methylobacterium sp.


organism: Methylorubrum extorquens DM4


organism: Synechocystis sp.

substrate_name: N-acetyl-D-mannosamine

substrate: N-acetyl-D-mannosamine


organism: Trichormus variabilis

substrate_name: N-acetyl-D-glucosamine

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

substrate: N-acetyl-D-glucosamine


organism: Homo sapiens


organism: Nostoc punctiforme


organism: Nostoc sp.


organism: Sus scrofa

assay_conditions_summary: cofactor ATP

proof_lines: in presence of saturating concentrations of ATP


substrate_name: ATP; L-Fucose

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

proof_lines:
pH 7.4, 37°C
pH 8.0, 37°C
ATP in form of MgATP2-

substrate: ATP; L-Fucose


organism: Caldanaerobius polysaccharolyticus

substrate_name: L-ribose

substrate: L-ribose


organism: Klebsiella aerogenes

substrate_name: mannose

substrate: mannose


organism: Paenibacillus baekrokdamisoli

substrate_name: D-allulose

assay_conditions_summary: pH 8

substrate: D-allulose


organism: Providencia stuartii

substrate_name: D-mannose

assay_conditions_summary: pH 7.5; 318.15 K; buffer 50 mM HEPES

substrate: D-mannose


organism: Bacillus thuringiensis

substrate_name: H2O; D-myo-Inositol 1,2-cyclic phosphate

sequence_source: UniProtKB:P08954 (active entry; sequence exists but is not emitted in this lane)


organism: Bacillus cereus

proof_lines: pH 7, 25°C, in presence of dihexanoylphosphatidylcholine

proof_lines: pH 7, 25°C, in absence of dihexanoylphosphatidylcholine


organism: Listeria monocytogenes

assay_conditions_summary: pH 7


enzyme_display_name: gluconate 2-dehydrogenase (acceptor)

organism: Serratia marcescens

substrate_name: D-gluconate

enzyme: gluconate 2-dehydrogenase (acceptor)

substrate: D-gluconate


organism: Gluconobacter oxydans


organism: Pseudomonas aeruginosa

assay_conditions_summary: pH 5


organism: Pseudomonas fluorescens

assay_conditions_summary: pH 6


enzyme_display_name: farnesyl diphosphatase

organism: Saccharolobus solfataricus

substrate_name: geranylgeranyl diphosphate

proof_lines:
pH 7, 37°C
pH 7, 60°C

enzyme: farnesyl diphosphatase

substrate: geranylgeranyl diphosphate


organism: Cricetulus griseus

substrate_name: (2E,6E)-farnesyl diphosphate

substrate: (2E,6E)-farnesyl diphosphate


organism: Oryza sativa


organism: Rattus norvegicus


organism: Bacillus subtilis (strain 168)

substrate_name: farnesyl pyrophosphate

substrate: farnesyl pyrophosphate


organism: Zea mays

substrate_name: geranyl diphosphate

substrate: geranyl diphosphate


organism: Homo sapiens

sequence_source: UniProtKB:P51878 accession-anchor check: reviewed human caspase-5 entry with a sequence present; sequence not adopted in this lane.


enzyme_display_name: N-carbamoylputrescine amidase

organism: Arabidopsis thaliana

substrate_name: H2O; N-Carbamoylputrescine

enzyme: N-carbamoylputrescine amidase

substrate: H2O; N-Carbamoylputrescine


substrate_name: N-Carbamoylputrescine

proof_lines: recombinant enzyme, pH and temperature not specified in the publication

substrate: N-Carbamoylputrescine


organism: Bacteroides thetaiotaomicron


organism: Selenomonas ruminantium


enzyme_display_name: tRNA(Met) cytidine acetyltransferase

organism: Escherichia coli

substrate_name: ATP; H2O

enzyme: tRNA(Met) cytidine acetyltransferase

substrate: ATP; H2O


enzyme_display_name: allantoate deiminase

organism: Glycine max

substrate_name: H2O; Allantoate

enzyme: allantoate deiminase

substrate: H2O; Allantoate


enzyme_display_name: [amino-group carrier protein]--L-2-aminoadipate ligase

organism: Thermus thermophilus


enzyme: [amino-group carrier protein]--L-2-aminoadipate ligase


enzyme_display_name: galactose-6-phosphate isomerase

organism: Lactococcus lactis subsp. lactis

substrate_name: D-Tagatose 6-phosphate

enzyme: galactose-6-phosphate isomerase

substrate: D-Tagatose 6-phosphate


organism: Lactococcus lactis

substrate_name: D-allose

substrate: D-allose


organism: Staphylococcus aureus

substrate_name: D-tagatose 6-phosphate

substrate: D-tagatose 6-phosphate


enzyme_display_name: methylmalonyl-CoA epimerase

enzyme: methylmalonyl-CoA epimerase


organism: Cereibacter sphaeroides


organism: Pyrococcus horikoshii

substrate_name: methylmalonyl-CoA

substrate: methylmalonyl-CoA


enzyme_display_name: avenacosidase

organism: Avena sativa

substrate_name: avenacoside B

enzyme: avenacosidase

substrate: avenacoside B


substrate_name: p-nitrophenyl-beta-D-glucopyranoside (with native enzyme)

proof_lines: The activity increases with rising aggregation of the enzyme. kcat is 982000 sec(-1) with avenacosides as substrate. kcat is 3090 sec(-1) with p-nitrophenyl-beta-D-glucopyranoside as substrate. kcat is 290 sec(-1) with genistin as substrate


substrate_name: N-carbamoylputrescine

substrate: N-carbamoylputrescine


substrate_name: p-nitrophenyl-beta-D-glucopyranoside (with heteromultimeric recombinant enzyme)


enzyme_display_name: thermospermine synthase

substrate_name: S-Adenosylmethioninamine; Spermidine

sequence_source: UniProt Q5SK28 accession context checked directly; no row-level sequence payload is established.

enzyme: thermospermine synthase

substrate: S-Adenosylmethioninamine; Spermidine


enzyme_display_name: sedoheptulokinase

organism: Mus musculus

substrate_name: ATP; Sedoheptulose

sequence_source: UniProt Q9D5J6 accession context checked directly; no row-level sequence payload is established.

enzyme: sedoheptulokinase

substrate: ATP; Sedoheptulose


enzyme_display_name: (3S)-malyl-CoA thioesterase

organism: Haloarcula marismortui

substrate_name: L-Malyl-CoA; H2O

sequence_source: UniProt Q5V463 accession context checked directly; no row-level sequence payload is established.

enzyme: (3S)-malyl-CoA thioesterase

substrate: L-Malyl-CoA; H2O


sequence_source: UniProt Q5V0X0 accession context checked directly; no row-level sequence payload is established.


organism: Rhodobacter sphaeroides

sequence_source: UniProt D3JV05 accession context checked directly; no row-level sequence payload is established.


substrate_name: H2O; p-Nitrophenyl-beta-D-glucoside

sequence_source: UniProt Q9ZP27 and Q38786 accession context checked directly; no row-level sequence payload is established.

substrate: H2O; p-Nitrophenyl-beta-D-glucoside


enzyme_display_name: ADP-glyceromanno-heptose 6-epimerase

organism: Unknown

substrate_name: ADP-D-glycero-D-manno-heptose

assay_conditions_summary: cofactor ADP

enzyme: ADP-glyceromanno-heptose 6-epimerase

substrate: ADP-D-glycero-D-manno-heptose


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: 3,4-DHSA (at pH 8 and at 25 degrees Celsius)


enzyme_display_name: 2-amino-5-chlorophenol 1,6-dioxygenase

organism: Comamonas testosteroni

enzyme: 2-amino-5-chlorophenol 1,6-dioxygenase


organism: Streptosporangium sibiricum


enzyme_display_name: (R)-2-methylmalate dehydratase

organism: Methanocaldococcus jannaschii

substrate_name: H2O; Citraconate

enzyme: (R)-2-methylmalate dehydratase

substrate: H2O; Citraconate


organism: Escherichia coli


substrate_name: 7,8-Dihydroneopterin 3'-triphosphate

substrate: 7,8-Dihydroneopterin 3'-triphosphate


enzyme_display_name: anthranilate 1,2-dioxygenase (deaminating, decarboxylating)

organism: Pseudomonas chlororaphis subsp. aureofaciens

substrate_name: anthranilate

substrate: anthranilate


enzyme_display_name: N-methylcoclaurine 3'-monooxygenase

organism: Eschscholzia californica

substrate_name: (S)-N-methylcoclaurine

enzyme: N-methylcoclaurine 3'-monooxygenase

substrate: (S)-N-methylcoclaurine


enzyme_display_name: (S)-limonene 7-monooxygenase

organism: Perilla frutescens

substrate_name: (-)-limonene

enzyme: (S)-limonene 7-monooxygenase

substrate: (-)-limonene


organism: Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

proof_lines: KM values measured using the AntAB complex.

enzyme: anthranilate 1,2-dioxygenase (deaminating, decarboxylating)


proof_lines: kcat is 88 min(-1) with SibE-PCP-3H4MAA as substrate.


enzyme_display_name: oryzalexin E synthase

organism: Oryza sativa subsp. japonica

substrate_name: ent-sandaracopimaradien

proof_lines: kcat is 0.046 sec(-1) with ent-sandaracopimaradien as substrate. kcat is 0.020 sec(-1) with syn-stemodene as substrate.

enzyme: oryzalexin E synthase

substrate: ent-sandaracopimaradien


substrate_name: 7,8-dihydroneopterin 3'-triphosphate

substrate: 7,8-dihydroneopterin 3'-triphosphate


enzyme_display_name: ent-isokaurene C2/C3-hydroxylase

organism: Oryza sativa

substrate_name: ent-isokaurene

proof_lines: pH and temperature not specified in the publication

enzyme: ent-isokaurene C2/C3-hydroxylase

substrate: ent-isokaurene


enzyme_display_name: ent-cassadiene hydroxylase

organism: Oryza sativa Japonica Group

proof_lines: pH not specified in the publication, temperature not specified in the publication

enzyme: ent-cassadiene hydroxylase


substrate_name: ent-cassadiene

proof_lines: kcat is 0.11 sec(-1) with ent-cassadiene as substrate.

substrate: ent-cassadiene


proof_lines: kcat is 0.01 sec(-1) with ent-isokaurene as substrate.


organism: Rhodococcus erythropolis

substrate_name: DTDB

substrate: DTDB


enzyme_display_name: plasminogen activator Pla

organism: Yersinia pestis

substrate_name: plasminogen

enzyme: plasminogen activator Pla

substrate: plasminogen


enzyme_display_name: pheophorbide a oxygenase

organism: Arabidopsis thaliana

substrate_name: pheophorbide a

enzyme: pheophorbide a oxygenase

substrate: pheophorbide a


enzyme_display_name: ferredoxin:thioredoxin reductase

organism: Spinacia oleracea

substrate_name: reduced ferredoxin

proof_lines: ferredoxin modified by treatment with glycine ethyl ester in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, pH 7.9

enzyme: ferredoxin:thioredoxin reductase

substrate: reduced ferredoxin


enzyme_display_name: (-)-pluviatolide 4-O-methyltransferase

organism: Sinopodophyllum hexandrum

substrate_name: (-)-pluviatolide

enzyme: (-)-pluviatolide 4-O-methyltransferase

substrate: (-)-pluviatolide


enzyme_display_name: arginine N(omega)-methyltransferase

organism: Streptomyces achromogenes subsp. streptozoticus

substrate_name: L-arginine

enzyme: arginine N(omega)-methyltransferase

substrate: L-arginine


enzyme_display_name: sesamin methylene transferase

organism: Sinomonas sp. No.22

substrate_name: (+)-sesamin

enzyme: sesamin methylene transferase

substrate: (+)-sesamin


organism: Mus musculus

substrate_name: docosahexaenoyl-CoA

substrate: docosahexaenoyl-CoA


enzyme_display_name: tyramine N-methyltransferase

organism: Hordeum vulgare

substrate_name: S-adenosyl-L-methionine

enzyme: tyramine N-methyltransferase

substrate: S-adenosyl-L-methionine


km_unit: mM

data_origin: experimental


organism: Coptis japonica

substrate_name: columbamine

substrate: columbamine


organism: Homo sapiens

substrate_name: lysophosphatidylethanolamine

substrate: lysophosphatidylethanolamine


enzyme_display_name: GDP-perosamine N-acetyltransferase

organism: Escherichia coli O157:H7

substrate_name: GDP-perosamine

enzyme: GDP-perosamine N-acetyltransferase

substrate: GDP-perosamine


organism: Petroselinum crispum


organism: Methanocaldococcus jannaschii

substrate_name: Unknown

substrate: Unknown


enzyme_display_name: phosphorylated butenolide synthase

organism: Streptomyces griseus

substrate_name: glycerone phosphate

enzyme: phosphorylated butenolide synthase

substrate: glycerone phosphate


enzyme_display_name: capsaicin synthase

organism: Capsicum annuum

assay_conditions_summary: pH 6.8; 310.15 K; cofactor CoA

enzyme: capsaicin synthase


organism: Streptomyces kanamyceticus


organism: Thermotoga maritima

substrate_name: GDP-alpha-D-mannose

substrate: GDP-alpha-D-mannose


enzyme_display_name: (Kdo)-lipid IVA 3-deoxy-D-manno-octulosonic acid transferase

organism: Escherichia coli


enzyme_display_name: homogentisate phytyltransferase

substrate_name: Phytyl diphosphate; Homogentisate

enzyme: homogentisate phytyltransferase

substrate: Phytyl diphosphate; Homogentisate


organism: Synechocystis sp.


organism: Gallus gallus

substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


enzyme_display_name: histidine N-acetyltransferase

organism: Oreochromis niloticus

substrate_name: acetylcoenzyme A

enzyme: histidine N-acetyltransferase

substrate: acetylcoenzyme A


enzyme_display_name: lysyltransferase

organism: Staphylococcus aureus

substrate_name: phosphatidylglycerol

enzyme: lysyltransferase

substrate: phosphatidylglycerol


enzyme_display_name: diglucosyl diacylglycerol synthase (1,2-linking)

organism: Acholeplasma laidlawii

substrate_name: UDP-glucose

substrate: UDP-glucose


enzyme: diglucosyl diacylglycerol synthase (1,2-linking)


substrate_name: UDP-alpha-D-galactose

substrate: UDP-alpha-D-galactose


organism: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

substrate_name: fructose-1,6-bisphosphate (at pH 7 and 50 degrees Celsius)


substrate_name: acetyl-CoA

substrate: acetyl-CoA


organism: Staphylococcus aureus (strain NCTC 8325 / PS 47)

substrate_name: L-lysyl-tRNA

substrate: L-lysyl-tRNA


substrate_name: UDP-Glc (alpha-diglucosyldiacylglycerol synthase activity at 28 degrees Celsius)


substrate_name: UDP-N-acetyl-alpha-D-glucosamine

substrate: UDP-N-acetyl-alpha-D-glucosamine


substrate_name: UDP-Gal

substrate: UDP-Gal


organism: Shigella boydii


organism: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

substrate_name: DIP

substrate: DIP


enzyme_display_name: xylosyl alpha-1,3-xylosyltransferase

organism: Homo sapiens

substrate_name: UDP-alpha-D-xylose

enzyme: xylosyl alpha-1,3-xylosyltransferase

substrate: UDP-alpha-D-xylose


organism: Escherichia coli (strain K12)

substrate_name: lipid IV(A) (at pH 8)

enzyme: (Kdo)-lipid IVA 3-deoxy-D-manno-octulosonic acid transferase

substrate: lipid IV(A) (at pH 8)


organism: Bacillus subtilis

substrate_name: UDPglucose

substrate: UDPglucose


substrate_name: UDP-D-xylose

substrate: UDP-D-xylose


organism: Sinopodophyllum hexandrum

substrate_name: (-)-pluviatolide

proof_lines: kcat is 0.72 sec(-1) with (-)-pluviatolide as substrate.

substrate: (-)-pluviatolide


organism: Sinomonas sp. (strain No.22)

substrate_name: sesamin

proof_lines: kcat is 7.9 sec(-1) with sesamin as substrate.

substrate: sesamin


organism: Streptomyces griseus subsp. griseus (strain JCM 4626 / CBS 651.72 / NBRC 13350 / KCC S-0626 / ISP 5235)

substrate_name: DHAP

substrate: DHAP


organism: Bacillus subtilis (strain 168)

substrate_name: UDP-alpha-D-glucose

substrate: UDP-alpha-D-glucose


organism: Cannabis sativa

substrate_name: olivetolic acid

substrate: olivetolic acid


organism: Mycobacterium tuberculosis


enzyme_display_name: tagatose kinase

organism: Agrobacterium fabrum (strain C58 / ATCC 33970)

substrate_name: D-tagatose

enzyme: tagatose kinase

substrate: D-tagatose


organism: Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)

substrate_name: D-glycero-D-manno-heptose 7-phosphate (at pH 7.4 and 30 degrees Celsius)

proof_lines: kcat is 0.48 sec(-1) for D-glycero-D-manno-heptose 7-phosphate.


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: ATP

proof_lines: kcat is 104 min(-1) for ATPase activity.

substrate: ATP


enzyme_display_name: quinolinate synthase

substrate_name: dihydroxyacetone phosphate

enzyme: quinolinate synthase

substrate: dihydroxyacetone phosphate


organism: Mycobacterium butyricum

assay_conditions_summary: pH 7.5; 295.15 K; cofactor ATP


substrate_name: olivetolate

substrate: olivetolate


enzyme_display_name: diacylglycerol kinase (CTP)

organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: CTP

enzyme: diacylglycerol kinase (CTP)

substrate: CTP


enzyme_display_name: spermosin

organism: Unknown

enzyme: spermosin


enzyme_display_name: mevalonate-3-phosphate 5-kinase

organism: Thermoplasma acidophilum

enzyme: mevalonate-3-phosphate 5-kinase


enzyme_display_name: pyridinol guanylyltransferase

organism: Methanocaldococcus jannaschii

substrate_name: GTP

proof_lines: with 3,6-dimethyl-2,4-dihydroxypyridine as cosubstrate, at pH 7.0 and 80°C

enzyme: pyridinol guanylyltransferase

substrate: GTP


enzyme_display_name: isoamyl acetate esterase

organism: Saccharomyces cerevisiae

substrate_name: 3-methylbutyl acetate

enzyme: isoamyl acetate esterase

substrate: 3-methylbutyl acetate


enzyme_display_name: carbendazim hydrolysing esterase

organism: Nocardioides sp. SG-4G

substrate_name: carbendazim

enzyme: carbendazim hydrolysing esterase

substrate: carbendazim


enzyme_display_name: maltose 6'-phosphate phosphatase

organism: Enterococcus faecalis

substrate_name: maltose 6'-phosphate

proof_lines: pH 7.0, temperature not specified in the publication

enzyme: maltose 6'-phosphate phosphatase

substrate: maltose 6'-phosphate


enzyme_display_name: galactan exo-1,6-beta-galactobiohydrolase (non-reducing end)

organism: Bifidobacterium longum subsp. Longum


organism: Pantoea agglomerans

proof_lines: 30°C, pH not specified in the publication


enzyme_display_name: 8-oxo-(d)GTP phosphatase

enzyme: 8-oxo-(d)GTP phosphatase


enzyme_display_name: 1'-carboxy-chondrochloren decarboxylase

organism: Chondromyces crocatus

substrate_name: 1'-carboxy-chondrochloren B

enzyme: 1'-carboxy-chondrochloren decarboxylase

substrate: 1'-carboxy-chondrochloren B


enzyme_display_name: geosmin synthase

organism: Streptomyces avermitilis

substrate_name: (2E,6E)-farnesyl diphosphate

enzyme: geosmin synthase

substrate: (2E,6E)-farnesyl diphosphate


enzyme_display_name: 3-amino-4-hydroxybenzoate synthase

organism: Streptomyces griseus

enzyme: 3-amino-4-hydroxybenzoate synthase


enzyme_display_name: GDP-perosamine N-acetyltransferase

organism: Escherichia coli

substrate_name: acetyl-CoA

assay_conditions_summary: pH 8; 310.15 K; cofactor CoA

enzyme: GDP-perosamine N-acetyltransferase

substrate: acetyl-CoA


enzyme_display_name: catalase peroxidase

organism: Mycobacterium tuberculosis

enzyme: catalase peroxidase


substrate_name: D-glucitol[side 1]

substrate: D-glucitol[side 1]


organism: Escherichia coli (strain K12)

substrate_name: D-galactitol

substrate: D-galactitol


enzyme_display_name: L-fuculokinase

substrate_name: L-fuculose

enzyme: L-fuculokinase

substrate: L-fuculose


enzyme_display_name: farnesyl-diphosphate kinase

organism: Bos taurus

enzyme: farnesyl-diphosphate kinase


organism: Homo sapiens

substrate_name: IDP

substrate: IDP


enzyme_display_name: oxepin-CoA hydrolase

substrate_name: oxepin-CoA (at 22 degrees Celsius and pH 8)

assay_conditions_summary: pH 8; cofactor CoA

enzyme: oxepin-CoA hydrolase


enzyme_display_name: Xaa-Arg dipeptidase

substrate_name: beta-alanyl-L-lysine

enzyme: Xaa-Arg dipeptidase

substrate: beta-alanyl-L-lysine


organism: Mus musculus


enzyme_display_name: tartrate decarboxylase

organism: Pseudomonas putida

substrate_name: D-malate

enzyme: tartrate decarboxylase

substrate: D-malate


organism: Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

substrate_name: FPP

substrate: FPP


enzyme_display_name: dimethylmaleate hydratase

organism: Eubacterium barkeri

substrate_name: dimethylmaleate

enzyme: dimethylmaleate hydratase

substrate: dimethylmaleate


organism: Citrobacter freundii

substrate_name: L-Fuculose

substrate: L-Fuculose


assay_conditions_summary: pH 7.8; 310.15 K; cofactor ATP


enzyme_display_name: mevalonate-3-phosphate 5-kinase

organism: Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

substrate_name: Unknown

enzyme: mevalonate-3-phosphate 5-kinase

substrate: Unknown


enzyme_display_name: pyridinol guanylyltransferase

organism: Methanococcus maripaludis (strain DSM 14266 / JCM 13030 / NBRC 101832 / S2 / LL)

enzyme: pyridinol guanylyltransferase


organism: Enterococcus faecalis (strain TX4000 / JH2-2)


organism: Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)

proof_lines: kcat is 59.1 sec(-1) for UDP-N,N'-diacetylbacillosamine (at pH 7.5 and 37 degrees Celsius).


organism: Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)

proof_lines: kcat is 261 sec(-1) with beta-1,6-Gal3 as substrate.

enzyme: galactan exo-1,6-beta-galactobiohydrolase (non-reducing end)


organism: Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1)

substrate_name: 8-oxo-dGTP (at pH 7.6 and 37 degrees Celsius as measured by the Pi sensor assay)

proof_lines: kcat is 0.18 sec(-1) with 8-oxo-dGTP as substrate measured by the Pi sensor assay. kcat is 0.29 sec(-1) with 8-oxo-dGTP as substrate measured by the Fiske-SubbaRow assay. kcat is 0.13 sec(-1) with 8-oxo-GTP as substrate.


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

proof_lines: kcat is 0.54 sec(-1) with 8-oxo-dGTP as substrate.


enzyme_display_name: pyrrole-2-carboxylate decarboxylase

organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

proof_lines: kcat is 35.8 sec(-1) with pyrrole-2-carboxylate as substrate.

enzyme: pyrrole-2-carboxylate decarboxylase


organism: Streptomyces griseus

substrate_name: 2-amino-4,5-dihydroxy-6-one-heptanoic acid-7-phosphate (at 30 degrees Celsius)

proof_lines: This Km value might be underestimated because the concentration of the substrate determined is perhaps lower than the actual concentration due to the instability of the substrate compound.


organism: Legionella pneumophila

substrate_name: UDP-N,N'-diacetylbacillosamine

substrate: UDP-N,N'-diacetylbacillosamine


organism: Fusarium fujikuroi

substrate_name: trans-farnesyl triphosphate

substrate: trans-farnesyl triphosphate


enzyme_display_name: spermosin

organism: Halocynthia roretzi

enzyme: spermosin


organism: Sus scrofa


organism: Escherichia coli

substrate_name: oxepin-CoA

assay_conditions_summary: cofactor CoA

proof_lines: pH and temperature not specified in the publication

substrate: oxepin-CoA


enzyme_display_name: verruculogen synthase

organism: Aspergillus fumigatus

substrate_name: fumitremorgin B

enzyme: verruculogen synthase

substrate: fumitremorgin B


enzyme_display_name: 3-hydroxyanthranilate oxidase

organism: Rattus norvegicus

enzyme: 3-hydroxyanthranilate oxidase


enzyme_display_name: fatty-acid peroxidase

organism: Arachis hypogaea

substrate_name: palmitate

enzyme: fatty-acid peroxidase

substrate: palmitate


organism: Lathyrus oleraceus

substrate_name: lauric acid

substrate: lauric acid


organism: Pseudomonas sp.

substrate_name: L-Tartrate

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NAD+

substrate: L-Tartrate


enzyme_display_name: (Ara-f)3-Hyp beta-L-arabinobiosidase

organism: Bifidobacterium longum

proof_lines: in 50 mM sodium acetate (pH 6.0), at 37°C

enzyme: (Ara-f)3-Hyp beta-L-arabinobiosidase


organism: Priestia megaterium

substrate_name: Pyrrole

substrate: Pyrrole


organism: Eubacterium barkeri


enzyme_display_name: uronolactonase

substrate_name: H2O; D-Glucurono-3,6-lactone

enzyme: uronolactonase

substrate: H2O; D-Glucurono-3,6-lactone


enzyme_display_name: isobutylamine N-monooxygenase

organism: Streptomyces viridifaciens

proof_lines: pH 7.5, 25°C, flavin reductase(FRED)/isobutylamine N-hydroxylase(IBAH) system

enzyme: isobutylamine N-monooxygenase


organism: Oospora sulphurea-ochracea


enzyme_display_name: anthocyanidin synthase

organism: Gerbera hybrid cultivar

substrate_name: (+)-catechin

enzyme: anthocyanidin synthase

substrate: (+)-catechin


enzyme_display_name: androst-4-ene-3,17-dione monooxygenase

organism: Ilyonectria destructans

substrate_name: testololactone

assay_conditions_summary: cofactor NADPH

enzyme: androst-4-ene-3,17-dione monooxygenase

substrate: testololactone


enzyme_display_name: nitrogenase (flavodoxin)

organism: Azotobacter vinelandii

substrate_name: flavodoxin hydroquinone

proof_lines: before and after reduction of the nitrogenase complex relative slow reactions take place, which limits the rate of the Fe protein cycle

enzyme: nitrogenase (flavodoxin)

substrate: flavodoxin hydroquinone


enzyme_display_name: beta-cyclopiazonate dehydrogenase

organism: Penicillium cyclopium

enzyme: beta-cyclopiazonate dehydrogenase


enzyme_display_name: selenate reductase (quinol)

organism: Enterobacter cloacae

substrate_name: selenate

enzyme: selenate reductase (quinol)

substrate: selenate


enzyme_display_name: (-)-lariciresinol reductase

organism: Camellia sinensis var. sinensis

substrate_name: (-)-lariciresinol

enzyme: (-)-lariciresinol reductase

substrate: (-)-lariciresinol


enzyme_display_name: isoflavone 7-O-methyltransferase

organism: Glycine max

assay_conditions_summary: pH 7.6; 303.15 K; cofactor CoA

enzyme: isoflavone 7-O-methyltransferase


organism: Shigella boydii


organism: Escherichia coli

substrate_name: [pyruvate formate-lyase]-glycine

substrate: [pyruvate formate-lyase]-glycine


enzyme_display_name: 6-hydroxymellein O-methyltransferase

organism: Daucus carota

substrate_name: Unknown

enzyme: 6-hydroxymellein O-methyltransferase


substrate_name: cytidine34 in tRNALeuCAA

proof_lines: wild-type tRNALeuCAA and diverse mutants thereof, pH 7.5, 37°C

substrate: cytidine34 in tRNALeuCAA


organism: Bacillus subtilis

substrate_name: adenine22 in tRNASer

sequence_source: UniProt P54471 identity context

proof_lines: recombinant enzyme, pH 8.0, 37°C, in vivo activity

proof_lines: recombinant enzyme, pH 8.0, 37°C, in vitro activity

substrate: adenine22 in tRNASer


enzyme_display_name: tRNA:m(4)X modification enzyme

organism: Saccharomyces cerevisiae

substrate_name: adenosine4 in tRNAHis

sequence_source: UniProt Q12383 identity context

enzyme: tRNA:m(4)X modification enzyme

substrate: adenosine4 in tRNAHis


enzyme_display_name: GDP-perosamine N-formyltransferase

organism: Brucella melitensis

substrate_name: GDP-alpha-D-perosamine

sequence_source: UniProt Q9ZHX0 redirects to F8WJP6; identity context only

proof_lines: mutant enzyme D78A/F142A, at pH 8.0, temperature not specified in the publication

enzyme: GDP-perosamine N-formyltransferase

substrate: GDP-alpha-D-perosamine


assay_conditions_summary: pH 6.5; 308.15 K; cofactor CoA


enzyme_display_name: 2-phosphonomethylmalate synthase

organism: Streptomyces hygroscopicus

substrate_name: acetyl-CoA

enzyme: 2-phosphonomethylmalate synthase

substrate: acetyl-CoA


enzyme_display_name: mannosylglycerate hydrolase

organism: Rubrobacter radiotolerans

substrate_name: mannosylglycerate (at 50 degrees Celsius)

enzyme: mannosylglycerate hydrolase


organism: Selaginella moellendorffii

substrate_name: 2-O-(alpha-D-mannosyl)-glycerate (at 40 degrees Celsius)


organism: Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27)

substrate_name: mannosylglycerate (at 70 degrees Celsius)


organism: Lathyrus oleraceus

substrate_name: pyridoxamine

substrate: pyridoxamine


enzyme_display_name: luteolin 7-O-glucuronosyltransferase

organism: Secale cereale

substrate_name: UDPglucuronic acid

enzyme: luteolin 7-O-glucuronosyltransferase

substrate: UDPglucuronic acid


substrate_name: luteolin 7-O-glucuronide

substrate: luteolin 7-O-glucuronide


substrate_name: poly(ribitol phosphate)

proof_lines:
molar concentration of the polymer
concentration as ribitol units

substrate: poly(ribitol phosphate)


organism: Homo sapiens


organism: Rattus norvegicus


enzyme_display_name: arylamine glucosyltransferase

substrate_name: UDPglucose

enzyme: arylamine glucosyltransferase

substrate: UDPglucose


enzyme_display_name: kaempferol 3-O-xylosyltransferase

organism: Euonymus alatus

substrate_name: UDP-alpha-D-xylose

proof_lines:
pH 7.5, 30°C, cosubstrate: kaempferol
pH 7.5, 30°C

enzyme: kaempferol 3-O-xylosyltransferase

substrate: UDP-alpha-D-xylose


enzyme_display_name: aminodeoxyfutalosine synthase

organism: Thermus thermophilus

enzyme: aminodeoxyfutalosine synthase


enzyme_display_name: lombricine kinase

organism: Megascolides cameroni

substrate_name: D-lombricine

enzyme: lombricine kinase

substrate: D-lombricine


enzyme_display_name: thiamine-diphosphate kinase

organism: Bos taurus

substrate_name: ATP

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

enzyme: thiamine-diphosphate kinase

substrate: ATP


organism: Cavia porcellus

assay_conditions_summary: cofactor ATP


enzyme_display_name: serine-phosphoethanolamine synthase

organism: Gallus gallus

substrate_name: L-serine

enzyme: serine-phosphoethanolamine synthase

substrate: L-serine


enzyme_display_name: bis(2-ethylhexyl)phthalate esterase

organism: Achromobacter denitrificans

substrate_name: 4-nitrophenyl acetate

enzyme: bis(2-ethylhexyl)phthalate esterase

substrate: 4-nitrophenyl acetate


organism: Triticum aestivum


enzyme_display_name: glutathione thiolesterase

organism: Spinacia oleracea

substrate_name: S-butyrylglutathione

enzyme: glutathione thiolesterase

substrate: S-butyrylglutathione


substrate_name: D-serine-phosphoethanolamine

substrate: D-serine-phosphoethanolamine


enzyme_display_name: mannan exo-1,2-1,6-alpha-mannosidase

organism: Cellulomonas sp.

substrate_name: D-mannan

proof_lines: pH 7, 37°C, hydrolysis of alpha-1,2-linkage in yeast mannan

proof_lines: pH 7, 37°C, hydrolysis of alpha-1,6-linkage in yeast mannan

proof_lines: pH 7, 37°C, hydrolysis of alpha-1,3-linkage in yeast mannan

enzyme: mannan exo-1,2-1,6-alpha-mannosidase

substrate: D-mannan


enzyme_display_name: cyclic 2,3-diphosphoglycerate hydrolase

organism: Methanothermobacter thermautotrophicus

substrate_name: cyclic 2,3-bisphosphoglycerate

enzyme: cyclic 2,3-diphosphoglycerate hydrolase

substrate: cyclic 2,3-bisphosphoglycerate


organism: Alteromonas sp. SN-1009

substrate_name: Kjellmaniella crassifolia fucoidan

proof_lines:
pH 7.0, 35°C
kcat/Km: 0.21 ml/mg*min, pH 7.0, 35°C

substrate: Kjellmaniella crassifolia fucoidan


enzyme_display_name: Pup amidohydrolase

organism: Mycolicibacterium smegmatis

enzyme: Pup amidohydrolase


enzyme_display_name: firefly luciferase

organism: Photinus pyralis

substrate_name: D-luciferin

assay_conditions_summary: pH 8.6; 298.15 K; cofactor ATP

proof_lines:
wild-type enzyme
pH 8.6, wild-type enzyme
25°C, pH 8, wild-type

enzyme: firefly luciferase

substrate: D-luciferin


organism: Luciola mingrelica

substrate_name: luciferin

substrate: luciferin


organism: Lampyris turkestanicus

assay_conditions_summary: pH 7.8; buffer 50 mM Tricine-NaOH; cofactor ATP

proof_lines: at pH 7.8, in 50 mM Tricine-NaOH buffer with 10 mM MgSO4

proof_lines: in 50 mM Tricine-NaOH buffer with 10 mM MgSO4, at pH 7.8


proof_lines: pH and temperature not specified in the publication, wild-type enzyme


organism: Nipponoluciola cruciata

substrate_name: lauric acid

substrate: lauric acid


substrate_name: Dehydroluciferyladenylate; CTP

assay_conditions_summary: pH 7.5; 298.15 K; cofactor ATP

proof_lines:
pH 7.5, 25°C
native luciferase
recombinant luciferase

substrate: Dehydroluciferyladenylate; CTP


assay_conditions_summary: pH 7.8; 295.15 K; cofactor ATP

proof_lines: pH 7.8, temperature not specified in the publication, wild-type enzyme

proof_lines: pH 7.8, 22°C, recombinant wild-type enzyme


enzyme_display_name: phosphogluconate dehydratase

organism: Caulobacter vibrioides

assay_conditions_summary: pH 8

enzyme: phosphogluconate dehydratase


enzyme_display_name: Transferred entry: 4.8.1.2

organism: Pseudomonas chlororaphis

substrate_name: n-Butyraldoxime

enzyme: Transferred entry: 4.8.1.2

substrate: n-Butyraldoxime


enzyme_display_name: 4,5-dihydroxyphthalate decarboxylase

organism: Comamonas testosteroni

enzyme: 4,5-dihydroxyphthalate decarboxylase


organism: Pseudomonas fluorescens


enzyme_display_name: ricinine nitrilase

organism: Pseudomonas sp.

enzyme: ricinine nitrilase


organism: Methylococcus capsulatus

substrate_name: D-Arabino-6-hexulose 3-phosphate

substrate: D-Arabino-6-hexulose 3-phosphate


organism: Pseudomonas putida


organism: Zymomonas mobilis


enzyme_display_name: L-rhamnose isomerase

organism: Stutzerimonas stutzeri

substrate_name: D-allose

assay_conditions_summary: pH 9; 308.15 K; buffer 50 mM glycine-NaOH

proof_lines: mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C

enzyme: L-rhamnose isomerase

substrate: D-allose


proof_lines: mutant enzyme H101N, pH and temperature not specified in the publication


organism: Thermoanaerobacterium saccharolyticum

substrate_name: L-Mannose

assay_conditions_summary: pH 7; 338.15 K; buffer and 1 mM Co2+ at pH 7

proof_lines: at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0

substrate: L-Mannose


organism: Thermobacillus composti


organism: Thermoclostridium stercorarium


enzyme_display_name: sucrose phosphorylase

organism: Bifidobacterium longum

substrate_name: phosphate

enzyme: sucrose phosphorylase

substrate: phosphate


organism: Leuconostoc mesenteroides

substrate_name: sucrose

proof_lines: pH 7.0, 30°C, phosphorolysis, recombinant enzyme

proof_lines: pH 7.0, 30°C, synthesis, recombinant enzyme

substrate: sucrose


organism: Leuconostoc mesenteroides subsp. mesenteroides


organism: Thermobacillus sp. ZCTH02-B1


organism: uncultured bacterium


organism: Bacillus cereus


organism: Lactiplantibacillus plantarum subsp. plantarum

substrate_name: isomaltose

proof_lines: pH 5.5, 30°C, recombinant His-tagged enzyme

substrate: isomaltose


organism: Saccharomyces cerevisiae

substrate_name: methyl alpha-D-glucopyranoside

proof_lines:
pH 7.0, 30°C
Raw SKiD Env: Temp=30°C, pH=7
Raw SKiD Env: Temp=30°C, pH=6.8

substrate: methyl alpha-D-glucopyranoside


organism: Suncus murinus

substrate_name: H2O; Isomaltose

substrate: H2O; Isomaltose


organism: Aeribacillus pallidus

substrate_name: L-rhamnose

substrate: L-rhamnose


organism: Bacillus subtilis


organism: Caldicellulosiruptor obsidiansis

assay_conditions_summary: pH 7


organism: Caldicellulosiruptor saccharolyticus

assay_conditions_summary: pH 7; 338.15 K; buffer 50 mM potassium phosphate

proof_lines: in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 65°C


organism: Dictyoglomus turgidum


organism: Pseudomonas stutzeri

substrate_name: D-ribose

proof_lines:
Raw SKiD Env: Temp=35°C, pH=9
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=50°C, pH=9

substrate: D-ribose


organism: Stutzerimonas stutzeri

proof_lines: 50°C, pH 9.0, intrinsic enzyme from Pseudomonas stutzeri


proof_lines: wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C

proof_lines: wild type enzyme, pH and temperature not specified in the publication


proof_lines: mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C


proof_lines: mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C


proof_lines: mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C


organism: Homo sapiens

substrate_name: maltose

substrate: maltose


organism: Rattus norvegicus

substrate_name: maltose (with stSI)

substrate: maltose (with stSI)


organism: Bifidobacterium breve

substrate_name: L-ascorbic acid

substrate: L-ascorbic acid


proof_lines: pH 4.5, 40°C, mutant enzyme P134C/L341V/L343F


organism: Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)

verification_status: corrected

proof_lines: kcat is 207 sec(-1) for the phosphorolysis of sucrose (at 58 degrees Celsius and pH 6.5).

status: corrected


organism: Bifidobacterium adolescentis


proof_lines: pH 7.0, 60°C, recombinant wild-type enzyme


organism: Leuconostoc mesenteroides


substrate_name: D-fructose

proof_lines:
30°C, pH 7.0, mutant enzyme D295E
pH 7.0, 30°C, synthesis, mutant D295E
pH 7.0, 30°C, arsenolysis, mutant D295E

substrate: D-fructose


substrate_name: alpha-D-glucose 1-phosphate

proof_lines: pH 7.0, 30°C, phosphorolysis, mutant D295N

substrate: alpha-D-glucose 1-phosphate


organism: Bifidobacterium dentium


proof_lines: pH 7.0, 30°C, phosphorolysis, mutant E237Q


proof_lines: arsenolysis of alpha-D-glucose 1-phosphate, mutant D196N/E237Q


proof_lines: pH 7.0, 30°C, sucrose phosphorolysis, recombinant wild-type enzyme

proof_lines: pH 7.0, 30°C, phosphorolysis, recombinant wild-type enzyme

proof_lines: pH 7.0, 30°C, sucrose synthesis by glycosylation from alpha-D-glucose 1-phosphate, recombinant wild-type enzyme


proof_lines: pH and temperature not specified in the publication


organism: Limosilactobacillus reuteri


organism: Roseateles saccharophilus


organism: Streptococcus mutans


organism: Zalophus californianus


organism: Pseudomonas sp.


substrate_name: L-Xylose

substrate: L-Xylose


organism: Thermotoga maritima

assay_conditions_summary: pH 8; 358.15 K; buffer 50 mM EPPS

proof_lines: purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)


organism: Bacillus subtilis

substrate_name: L-Lyxose

substrate: L-Lyxose


organism: Halalkalibacterium halodurans

assay_conditions_summary: pH 7; 343.15 K; buffer 50 mM malate

proof_lines: in 50 mM malate buffer, at pH 7.0 and 70°C


organism: Lactiplantibacillus plantarum


organism: Mesorhizobium loti

proof_lines: in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C


organism: Escherichia coli

substrate_name: L-Rhamnose

substrate: L-Rhamnose


enzyme_display_name: peptidylprolyl isomerase

organism: Arabidopsis thaliana

enzyme: peptidylprolyl isomerase


organism: Corynebacterium glutamicum

proof_lines: pH 7.5, temperature not specified in the publication


substrate_name: RNase T1

proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication

substrate: RNase T1


proof_lines: mutant Y15A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant I65P, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant A82P, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant V72P, pH not specified in the publication, temperature not specified in the publication


organism: Halobacterium salinarum


organism: Homo sapiens


substrate_name: Unknown

substrate: Unknown


organism: Thermus thermophilus

substrate_name: TRYWNAKMK-(cis)-PFIFGA

proof_lines: wild-type, pH 7.5, temperature not specified in the publication

substrate: TRYWNAKMK-(cis)-PFIFGA


proof_lines: mutant lacking the insert-in-flap domain, pH 7.5, temperature not specified in the publication


organism: Trypanosoma cruzi

assay_conditions_summary: pH 7.8; 283.15 K; buffer 35 mM sodium HEPES

proof_lines: at 10 °C in 35 mM sodium HEPES buffer, pH 7.8


enzyme_display_name: phenylpyruvate tautomerase

organism: Bos taurus

enzyme: phenylpyruvate tautomerase


substrate_name: keto-(p-hydroxyphenyl)pyruvate

substrate: keto-(p-hydroxyphenyl)pyruvate


organism: Mus musculus

proof_lines:
23°C, pH 6.5
Raw SKiD Env: Temp=23°C, pH=6.5
Raw SKiD Env: Temp=23°C, pH=-----


substrate_name: enol-(p-hydroxyphenyl)pyruvate

sequence_source: UniProtKB:P34884 (canonical identity context only)

proof_lines:
23°C, pH 6.5, mutant enzyme Y95F
23°C, mutant enzyme Y95F

substrate: enol-(p-hydroxyphenyl)pyruvate


substrate_name: enol-phenylpyruvate

proof_lines:
23°C, mutant enzyme P1A
23°C, pH 6.5, mutant enzyme P1A

substrate: enol-phenylpyruvate


proof_lines:
23°C, pH 6.5, mutant enzyme N97A
23°C, mutant enzyme N97A


proof_lines:
23°C, wild-type enzyme
23°C, pH 6.5, wild-type enzyme


proof_lines:
23°C, mutant enzyme P1G
23°C, pH 6.5, mutant enzyme P1G


proof_lines: wild type enzyme, with 50 mM dithiothreitol in 35 mM HEPES, pH 7.8, at 10°C


proof_lines: mutant enzyme, with 50 mM dithiothreitol in 35 mM HEPES, pH 7.8, at 10°C


proof_lines: mutant enzyme G148D, with 50 mM dithiothreitol in 35 mM HEPES, pH 7.8, at 10°C


organism: Mycoplasmoides genitalium

proof_lines: disulfide-reduced and S-carboxymethylated form of a variant of Rnase T1 with Ser54Gly and Pro55Asn


organism: Oryctolagus cuniculus

assay_conditions_summary: pH 7.4; 293.15 K; cofactor ATP

proof_lines: 20°C, pH 7.4, wild-type, presence of 1 mM MgATP


proof_lines: 20°C, pH 7.4, mutant DELTA208-213, presence of 1 mM MgATP


organism: Mycolicibacterium smegmatis

substrate_name: phenylenolpyruvate

assay_conditions_summary: pH 7.3; 295.15 K; buffer 10 mM Na2HPO4

proof_lines: wild type enzyme cis-CAAD, in 10 mM Na2HPO4 buffer, pH 7.3, at 22°C

proof_lines: wild type enzyme CCH2-P1A, in 10 mM Na2HPO4 buffer, pH 7.3, at 22°C

proof_lines: wild type enzyme CCH2, in 10 mM Na2HPO4 buffer, pH 7.3, at 22°C

substrate: phenylenolpyruvate


proof_lines: mutant enzyme CCH2-P1A, in 10 mM Na2HPO4 buffer, pH 7.3, at 22°C


organism: Saccharomyces cerevisiae

proof_lines: 20°C, pH 7.4, wild-type Ypa2, presence of 1 mM MgATP

proof_lines: 20°C, pH 7.4, wild-type Ypa1, presence of 1 mM MgATP


proof_lines: 20°C, pH 7.4, mutant D205G of Ypa1, presence of 1 mM MgATP


organism: Pseudomonas pavonaceae

assay_conditions_summary: pH 9


organism: Pseudomonas aeruginosa

substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

substrate: NADPH


organism: Streptomyces avermitilis

substrate_name: NADH

assay_conditions_summary: pH 6.2; 298.15 K; cofactor NADPH

proof_lines:
pH 6.2, 25°C
cosubstrate NADPH, pH 6.2, 25°C
cosubstrate NADH, pH 6.2, 25°C

substrate: NADH


organism: Acidobacteriota bacterium

substrate_name: thio-NAD+

assay_conditions_summary: pH 6.2; 325.15 K; cofactor NADPH

proof_lines:
cosubstrate NADPH, pH 6.2, 52°C
cosubstrate NADH, pH 6.2, 52°C
pH 6.2, 52°C

substrate: thio-NAD+


organism: Azotobacter vinelandii

substrate_name: thio-NADP+

assay_conditions_summary: 298.15 K; cofactor NADP+

proof_lines:
cosubstrate NADH
+ thio-NAD+
at 25°C

substrate: thio-NADP+


organism: Escherichia coli

assay_conditions_summary: pH 7.5; 308.15 K; cofactor NADPH


organism: Nocardia jiangxiensis

assay_conditions_summary: pH 5.7; 308.15 K; cofactor NADPH

proof_lines:
pH 5.7, 35°C
cosubstrate NADH, pH 5.7, 35°C
cosubstrate NADPH, pH 5.7, 35°C


organism: Bacillus subtilis


organism: Legionella pneumophila


organism: Vicia faba


enzyme_display_name: sucrose alpha-glucosidase

organism: Xanthomonas campestris pv. campestris

substrate_name: sucrose

enzyme: sucrose alpha-glucosidase

substrate: sucrose


organism: Homo sapiens

proof_lines: pH and temperature not specified in the publication


organism: Mus musculus


organism: Sus scrofa

substrate_name: phenylpyruvate

proof_lines:
keto-form, kidney
enol-form, kidney
thyroid gland

substrate: phenylpyruvate


substrate_name: PPIL1; nSKIP

proof_lines: using Trp-Phe-Tyr-pSer-Pro-Arg-4-nitroanilide as substrate, in 50 mM HEPES, 0.1 M NaCl, 5 mM NaN3, pH 7.4, at 22°C

substrate: PPIL1; nSKIP


enzyme_display_name: inulosucrase

organism: Lactobacillus johnsonii

substrate_name: Sucrose; (2,1-beta-D-Fructosyl)n

enzyme: inulosucrase

substrate: Sucrose; (2,1-beta-D-Fructosyl)n


organism: Lactobacillus reuteri


organism: Suncus murinus

substrate_name: H2O; Maltose

substrate: H2O; Maltose


organism: Xanthomonas axonopodis

proof_lines:
pH 7.5, 30°C
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=30°C, pH=7.5


enzyme_display_name: poly(3-hydroxybutyrate) depolymerase

organism: Acidovorax sp.

proof_lines:
linear 3-hydroxybutyrate oligomer
cyclic 3-hydroxybutyrate oligomer
linear 3-hydroxybutyrateoligomer

enzyme: poly(3-hydroxybutyrate) depolymerase


organism: Cupriavidus necator

proof_lines:
linear 3-hydroxybutyrate oligomer
cyclic 3-hydroxybutyrate oligomer


organism: Rhodospirillum rubrum


organism: Streptomyces sp.


organism: Ralstonia pickettii

substrate_name: 4-nitrophenyl pentanoate

proof_lines: recombinant mutant A66V/N285D/G310G, pH 7.0, 30°C

substrate: 4-nitrophenyl pentanoate


substrate_name: 4-nitrophenyl hexanoate

proof_lines: recombinant mutant T145S/S246T/T252T/S257T, pH 7.0, 30°C

substrate: 4-nitrophenyl hexanoate


substrate_name: 4-nitrophenyl acetate

proof_lines: recombinant mutant T37A/N285Y, pH 7.0, 30°C

substrate: 4-nitrophenyl acetate


proof_lines: recombinant mutant A66V/N251S/T290A, pH 7.0, 30°C


proof_lines: recombinant mutant S92S/G180D/I292F, pH 7.0, 30°C


organism: Thermobifida sp. BCC23166

substrate_name: p-nitrophenyl butyrate

substrate: p-nitrophenyl butyrate


organism: Burkholderia pickettii

substrate_name: H2O; Poly[(R)-3-hydroxybutanoate]n

substrate: H2O; Poly[(R)-3-hydroxybutanoate]n


substrate_name: H2O; Poly[(R)-3-hydroxybutanoate]n-x

substrate: H2O; Poly[(R)-3-hydroxybutanoate]n-x


organism: Lihuaxuella thermophila


substrate_name: 4-nitrophenyl butyrate

substrate: 4-nitrophenyl butyrate


proof_lines: recombinant mutant T37A/N285D, pH 7.0, 30°C


proof_lines: recombinant mutant S92S/T145S/N285Y, pH 7.0, 30°C


proof_lines: recombinant mutant G108G/Q125L/R230R/K260M/T284S/G310G, pH 7.0, 30°C


proof_lines: recombinant wild-type enzyme, pH 7.0, 30°C


proof_lines: recombinant mutant A66V/T145S/N285D, pH 7.0, 30°C


proof_lines: recombinant mutant T252T/S257T/N285D, pH 7.0, 30°C


organism: Acidovorax sp. sa1


enzyme_display_name: morphine 6-dehydrogenase

substrate_name: morphine

enzyme: morphine 6-dehydrogenase

substrate: morphine


organism: Mesocricetus auratus

assay_conditions_summary: cofactor NAD+

proof_lines: similar values of other 17beta-hydroxysteroids


organism: Oryctolagus cuniculus

substrate_name: codeine

proof_lines:
with NAD+
cosubstrate NAD+

substrate: codeine


organism: Pseudomonas putida

assay_conditions_summary: cofactor NADP+

proof_lines:
cosubstrate NADP+
cosubstrate NADPH
with NADP+


organism: Rattus norvegicus

proof_lines: cosubstrate NAD+, in the female adult rats

proof_lines: cosubstrate NAD+, in the male adult rats


enzyme_display_name: peptidyl-Lys metalloendopeptidase

organism: Armillaria mellea

substrate_name: SAQKRVS

proof_lines: recombinant wild-type enzyme, pH 7.5, temperature not specified in the publication

enzyme: peptidyl-Lys metalloendopeptidase

substrate: SAQKRVS


organism: Grifola frondosa


enzyme_display_name: UDP-sugar diphosphatase

organism: Sus scrofa

substrate_name: H2O; UDPglucose

enzyme: UDP-sugar diphosphatase

substrate: H2O; UDPglucose


organism: Cavia porcellus

substrate_name: NAD+; Morphine

proof_lines:
cosubstrate NAD+
cosubstrate NADP+
with NADP+

substrate: NAD+; Morphine


organism: Unknown

substrate_name: GDP-L-fucose

substrate: GDP-L-fucose


organism: Homo sapiens

proof_lines: pH and temperature not specified in the publication


organism: Mus musculus

substrate_name: GDP-L-Fuc

proof_lines:
pH 7.5, 37°C
Raw SKiD Env: Temp=37°C, pH=7.5

substrate: GDP-L-Fuc


organism: Methanothermobacter thermautotrophicus

assay_conditions_summary: pH 8; 338.15 K; cofactor CoA


organism: Pyrococcus sp.

substrate_name: pyruvate

assay_conditions_summary: pH 6.9; 358.15 K; cofactor CoA

substrate: pyruvate


organism: Thermococcus litoralis

substrate_name: hydroxyphenylpyruvate

proof_lines:
pH 6.9, 85°C, under argon
pH 7.0, 85°C, under argon

substrate: hydroxyphenylpyruvate


organism: Cricetulus griseus

substrate_name: factor VII EGF-1 domain

substrate: factor VII EGF-1 domain


enzyme_display_name: ferric-chelate reductase (NADH)

organism: Beta vulgaris

substrate_name: NADH; H+; Fe3+

assay_conditions_summary: cofactor NADH

enzyme: ferric-chelate reductase (NADH)

substrate: NADH; H+; Fe3+


substrate_name: Fe(III)-nitrilotriacetic acid[side 2]

proof_lines: in 25 mM MOPS, 25 mM MES, 5.4 mM KCl, 5 mM glucose, 140 mM NaCl, 1.8 mM CaCl2, 0.8 mM MgCl2, pH 7.0, at 37°C

proof_lines: in 25 mM MOPS, 25 mM MES, 5.4 mM KCl, 5 mM glucose, 140 mM NaCl, 1.8 mM CaCl2, 0.8 mM MgCl2, pH 7.0, at 22°C

substrate: Fe(III)-nitrilotriacetic acid[side 2]


substrate_name: ferricytochrome b5

proof_lines: short ferricytochrome b5 from rabbit microsomes, at pH 6.5 and 30°C

proof_lines: long ferricytochrome b5 from pig microsomes, at pH 6.5 and 30°C

proof_lines: long ferricytochrome b5 from rabbit microsomes, at pH 6.5 and 30°C

substrate: ferricytochrome b5


proof_lines: short ferricytochrome b5 from pig microsomes, at pH 6.5 and 30°C

proof_lines: long ferricytochrome b5 from rat microsomes, at pH 6.5 and 30°C


proof_lines: short ferricytochrome b5 from rat microsomes, at pH 6.5 and 30°C


enzyme_display_name: protein-histidine pros-kinase

organism: Saccharomyces cerevisiae

substrate_name: ATP

assay_conditions_summary: cofactor ATP

enzyme: protein-histidine pros-kinase

substrate: ATP


enzyme_display_name: chitin disaccharide deacetylase

organism: Pyrococcus horikoshii

substrate_name: N,N'-diacetylchitobiose

enzyme: chitin disaccharide deacetylase

substrate: N,N'-diacetylchitobiose


organism: Shewanella baltica


organism: Thermococcus kodakarensis


organism: Vibrio parahaemolyticus


proof_lines: pH 7.0, 37°C, recombinant mutant enzyme lacking the carbohydrate-binding domains


organism: Rattus norvegicus


enzyme_display_name: yapsin 1

substrate_name: Adrenocorticotropic hormone 1-39; H2O

enzyme: yapsin 1

substrate: Adrenocorticotropic hormone 1-39; H2O


organism: Archaeoglobus fulgidus


organism: uncultured archaeon


enzyme_display_name: arginine N(omega)-methyltransferase

organism: Streptomyces achromogenes subsp. streptozoticus

substrate_name: L-arginine

proof_lines: kcat is 5.9 min(-1) with L-arginine as substrate.

enzyme: arginine N(omega)-methyltransferase

substrate: L-arginine


enzyme_display_name: norajmaline N-methyltransferase

organism: Rauvolfia serpentina

substrate_name: S-adenosyl-L-methionine

proof_lines: kcat is 1.8 sec(-1) with S-adenosyl-L-methionine as substrate (PubMed:27122470). kcat is 2.1 sec(-1) with norajmaline as substrate (PubMed:27122470).

enzyme: norajmaline N-methyltransferase

substrate: S-adenosyl-L-methionine


enzyme_display_name: ajmaline N-methyltransferase

proof_lines: kcat is 0.7 sec(-1) with S-adenosyl-L-methionine as substrate (PubMed:27122470). kcat is 1 sec(-1) with norajmaline as substrate (PubMed:27122470).

enzyme: ajmaline N-methyltransferase