organism: Enterobacter cloacae


enzyme_display_name: factor independent urate hydroxylase

organism: Geobacillus thermocatenulatus

substrate_name: uric acid

source_db: brenda

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: 0.1 M sodium borate, pH 8.5 containing 2 mM uric acid, 30 mM 4-aminoantipyrine, 1.5% phenol and peroxidase (15 U/ml), 37°C, 20 min

enzyme: factor independent urate hydroxylase

substrate: uric acid

source: brenda


organism: Glycine max

substrate_name: Urate; O2; H2O

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

substrate: Urate; O2; H2O


organism: Metabacillus fastidiosus

proof_lines: native uricase, monomethoxypoly(ethylene glycol) N-leucine-OSu-uricase and branched monomethoxypoly(ethylene glycol) N-leucine-OSu-uricase


organism: Microbacterium sp.

substrate_name: Urate

substrate: Urate


organism: Penicillium purpurescens


organism: Sphingobacterium thalpophilum


organism: synthetic construct


organism: Uromyces phaseoli


organism: Uromyces viciae-fabae


enzyme_display_name: benzoate O-methyltransferase

organism: Antirrhinum majus

substrate_name: S-adenosyl-L-methionine

proof_lines: apparent Km-value of plant-purified protein, pH 7.5, 20°C

proof_lines: apparent Km-value of recombinant protein, pH 7.5, 20°C

proof_lines: for S-adenosyl-L-methionine, pH 7.5, 20°C

enzyme: benzoate O-methyltransferase

substrate: S-adenosyl-L-methionine


enzyme_display_name: phosphatidylinositol 3-kinase

organism: Bos taurus

substrate_name: ATP

assay_conditions_summary: cofactor ATP

enzyme: phosphatidylinositol 3-kinase

substrate: ATP


organism: Homo sapiens


organism: Mus musculus

verification_status: unverified

assay_conditions_summary: pH 7.4; cofactor ATP

status: unverified


organism: Rattus norvegicus

proof_lines: with phosphatidylinositol 4-phosphate or phosphatidylinositol 4,5-bisphosphate as cosubstrate

proof_lines: with phosphatidylinositol as cosubstrate


organism: Sus scrofa

substrate_name: phosphatidylinositol 4-phosphate

proof_lines: reaction with phosphatidylinositol 4,5-diphosphate

proof_lines: reaction with phosphatidylinositol 4-phosphate

substrate: phosphatidylinositol 4-phosphate


enzyme_display_name: thiamine-phosphate kinase

organism: Escherichia coli

substrate_name: thiamine phosphate

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

enzyme: thiamine-phosphate kinase

substrate: thiamine phosphate


organism: Pseudomonas aeruginosa

assay_conditions_summary: pH 8; cofactor ATP

proof_lines: pH 8.0, temperature not specified in the publication


substrate_name: UDP-N-acetyl-alpha-D-glucosamine

substrate: UDP-N-acetyl-alpha-D-glucosamine


organism: Neisseria meningitidis

protein_accession_database: UniProt

sequence_source: UniProtKB H2VFI5 FASTA, anchored by PMID 15518580 MC58 SiaA/NeuC abstract

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: wild-type, 37°C, pH not specified in the publication


proof_lines:
pH 7.5, 37°C
pH 7.5, 37°C, hexamer


enzyme_display_name: glucan endo-1,6-beta-glucosidase

organism: Acremonium persicinum

substrate_name: Pustulan; H2O

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

enzyme: glucan endo-1,6-beta-glucosidase

substrate: Pustulan; H2O


organism: Eupenicillium brefeldianum

substrate_name: H2O; Pustulan

substrate: H2O; Pustulan


organism: Fusarium fujikuroi

substrate_name: Gentiotetraose

proof_lines: assumed molecular weight for lutean 650000 Da

substrate: Gentiotetraose


organism: Trichoderma harzianum


organism: Verruciconidia persicina

substrate_name: pustulan

proof_lines: assumed molecular weight for pustulan 17000 Da, or KM of 1.28 mg/ml

substrate: pustulan


enzyme_display_name: acrosin

organism: Capra hircus

substrate_name: Nalpha-tosyl-L-arginine methyl ester

enzyme: acrosin

substrate: Nalpha-tosyl-L-arginine methyl ester


substrate_name: Unknown

km_display: Not reported

ki_unit: mM

substrate: Unknown


organism: Ovis aries


enzyme_display_name: nitrate reductase (cytochrome)

organism: Desulfovibrio desulfuricans (strain ATCC 27774 / DSM 6949 / MB)

substrate_name: nitrate (in the presence of NaCl)

source_db: uniprot

data_origin: experimental

enzyme: nitrate reductase (cytochrome)

substrate: nitrate (in the presence of NaCl)

source: uniprot


organism: Oryza sativa subsp. japonica


organism: Pisum sativum

substrate_name: galactinol for the raffinose synthesis activity

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


organism: Campylobacter jejuni

substrate_name: nitrate

sequence_variant_note: Resolved canonical sequence from UniProtKB:Q5HV12 direct lookup plus mutation reconstruction and applied mutation signature C176S to reconstruct the assayed variant sequence.

mutation_type: substitution

substrate: nitrate


source_db: oed

kcat_over_km_origin: calculated

sequence_source: UniProtKB:Q5HV12 direct lookup

source: oed


organism: Cereibacter sphaeroides f. sp. denitrificans

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

proof_lines:
mutant K56H, 25°C, pH 6
mutant K56H, 25°C, pH 5


sequence_source: UniProtKB:O88111 direct lookup

proof_lines:
wild-type, 25°C, pH 6
wild-type, 25°C, pH 5
wild-type, 25°C, pH 7


proof_lines:
mutant K56M, 25°C, pH 6
mutant K56M, 25°C, pH 7


sequence_source: UniProtKB:O88111 direct lookup selected from source accession bundle


organism: Arabidopsis thaliana

sequence_source: UniProtKB:Q9SYJ4 direct lookup

proof_lines: pH 7.0, 25°C, recombinant His-tagged enzyme


organism: Beta vulgaris

substrate_name: sucrose; galactinol

sequence_source: UniProtKB:A0A1S7IYZ8 direct lookup

substrate: sucrose; galactinol


organism: Lathyrus oleraceus

substrate_name: galactinol; sucrose

sequence_source: UniProtKB:Q8VWN6 direct lookup

proof_lines: partially purified native enzyme, raffinose synthesis

proof_lines: recombinant enzyme in Sf21 cell extract, raffinose synthesis

substrate: galactinol; sucrose


enzyme_display_name: exoribonuclease II

organism: Escherichia coli

substrate_name: Poly(A)

sequence_variant_note: Resolved canonical sequence from Reconstructed E542A from UniProtKB reviewed canonical sequence P30850 (RNB_ECOLI), direct REST lookup and applied mutation signature E542A to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 8; 310.15 K; buffer 20 mM Tris-HCl

proof_lines: 35-nt poly(A), mutant E542A, at 37°C, in 20 mM Tris-HCl buffer, pH 8, 100 mM KCl, 1 mM MgCl2, and 1 mM dithiothreitol

enzyme: exoribonuclease II

substrate: Poly(A)


sequence_source: UniProtKB reviewed canonical sequence P30850 (RNB_ECOLI), direct REST lookup

proof_lines: 35-nt poly(A), wild-type, at 37°C, in 20 mM Tris-HCl buffer, pH 8, 100 mM KCl, 1 mM MgCl2, and 1 mM dithiothreitol


organism: Escherichia coli (strain K12)

substrate_name: S-adenosylmethionine

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

substrate: S-adenosylmethionine


organism: Achromobacter sp. MMT


organism: Cupriavidus necator


organism: Paracoccus pantotrophus

assay_conditions_summary: pH 7

proof_lines: pH 7, NapAB catalysed nitrate reduction driven by direct electron transfer from the electrode to NapAB, protein film voltammetry


organism: Oryza sativa


organism: Vicia faba

proof_lines:
+ sucrose
cosubstrate galactinol
+ sucrose, exchange reaction


substrate_name: polyadenosine

proof_lines: malE-malF mRNA transcripts incubated at 37°C

substrate: polyadenosine


organism: Homo sapiens


enzyme_display_name: methionine transaminase

substrate_name: indole-3-pyruvic acid (at pH 8.5 and 37 degrees Celsius)

proof_lines: kcat is 2.5 min(-1) with indole-3-pyruvic acid as substrate. kcat is 1.9 min(-1) with L-Met as substrate. kcat is 3.16 min(-1) with L-Phe as substrate (at pH 8.5 and 37 degrees Celsius).

enzyme: methionine transaminase


substrate_name: 2-oxo acid 4-methylthio-2-oxobutyrate (MTOB)


substrate_name: 4-methyl-2-oxopentanoate (4MOP)

substrate: 4-methyl-2-oxopentanoate (4MOP)


substrate_name: S-adenosyl-L-methionine

proof_lines: in 100 mM glycylglycine (pH 8.7), 100 mM EDTA, 100 mM KCl, 0.5 mM dithiothreitol, at 37°C

proof_lines: competitive inhibition against 7-(aminomethyl)-7-deazaguanine-tRNATyr, in 100 mM glycylglycine (pH 8.7), 100 mM KCl, 0.5 mM dithiothreitol, 100 mM EDTA, at 37°C

substrate: S-adenosyl-L-methionine


enzyme_display_name: histidine kinase

organism: Anaeromyxobacter sp.

substrate_name: ATP

sequence_source: UniProtKB:A7HD43 REST JSON direct lookup; reviewed Swiss-Prot; organism Anaeromyxobacter sp. (strain Fw109-5); EC 2.7.13.3

assay_conditions_summary: pH 8; 293.15 K; cofactor ATP

enzyme: histidine kinase

substrate: ATP


sequence_source: UniProtKB:P23837 REST JSON direct lookup; reviewed Swiss-Prot; organism Escherichia coli (strain K12); EC 2.7.13.3

assay_conditions_summary: pH 8; 295.15 K; cofactor ATP


organism: Bos taurus


substrate_name: ATP (at 22 degrees Celsius and pH 8, in the presence of 50 mM KCl and 1 mM MgCl(2))


substrate_name: IDP

substrate: IDP


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)


substrate_name: ATP for autophosphorylation by deoxy-DosT


substrate_name: ATP for autophosphorylation by deoxy-DevS


organism: Rattus norvegicus


organism: Solanum tuberosum


organism: Anaeromyxobacter sp. (strain Fw109-5)

substrate_name: ATP (with the Fe(III)-OH(-) bound form of the enzyme)

sequence_source: source_record; UniProt REST FASTA A7HD43 matched selected sequence

proof_lines: kcat is 1.1 min(-1) for the autophosphorylation reaction with the Fe(III)-OH(-) bound form of the enzyme. kcat is 1.2 min(-1) with the Fe(III)-CN(-) bound form of the enzyme. kcat is 1.1 min(-1) with the Fe(III)-imidazole bound form of the


organism: Staphylococcus aureus

sequence_source: source_record; UniProt REST FASTA Q9RDT3 matched selected sequence

sequence_variant_note: Mutation context was captured, but the signature could not be translated into an exact residue substitution safely.

mutation_type: truncation

proof_lines: Kinetic parameters were determined with a truncated form of WalK, which includes only the cytoplasmic domain and not the sensor domain and the transmembrane region.


organism: Streptococcus pneumoniae (strain ATCC BAA-255 / R6)

substrate_name: ATP (at pH 7.8 and 25 degrees Celsius)

sequence_source: source_record; UniProt REST FASTA Q8DPL8 matched selected full-entry sequence

assay_conditions_summary: pH 7.8; cofactor ATP

proof_lines: Kinetic parameters were determined with a truncated form of WalK, which includes only the cytoplasmic domain and not the short extracellular domain and the transmembrane region. kcat is 0.08 sec(-1) for ATP (at pH 7.8 and 25 degrees Celsius

substrate: ATP (at pH 7.8 and 25 degrees Celsius)


organism: Mycobacterium tuberculosis

verification_status: corrected

sequence_source: UniProtKB:O07778 HK1_MYCTU; direct UniProt FASTA check; BRENDA literature 679855 and PubMed 17434492 identify HK1.

status: corrected


organism: Myxococcus xanthus

sequence_variant_note: Resolved canonical sequence from UniProtKB:Q4VPT3 rodK canonical sequence with D657N/D782N/D909N reconstructed after direct position check. and applied mutation signature D657N/D782N/D909N to reconstruct the assayed variant sequence.


organism: Thermotoga maritima

substrate_name: Unknown


enzyme_display_name: cholesterol 7alpha-monooxygenase

substrate_name: cholesterol

proof_lines:
isoenzyme I
isoenzyme II
reconstituted enzyme complex

enzyme: cholesterol 7alpha-monooxygenase

substrate: cholesterol


enzyme_display_name: uroporphyrinogen-III synthase

substrate_name: hydroxymethylbilane

enzyme: uroporphyrinogen-III synthase

substrate: hydroxymethylbilane


substrate_name: O2; NADPH; H+; Cholesterol

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

sequence_source: UniProtKB reviewed accession P22680; direct lookup 2026-06-25

assay_conditions_summary: pH 7.2; 310.15 K; cofactor NADPH

substrate: O2; NADPH; H+; Cholesterol


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed accession P22680; direct lookup 2026-06-25 and applied mutation signature T104L to reconstruct the assayed variant sequence.


substrate_name: Cholesterol; O2; NADPH; H+

sequence_source: UniProtKB reviewed accession P18125; direct lookup 2026-06-25

assay_conditions_summary: cofactor NADPH

substrate: Cholesterol; O2; NADPH; H+


organism: Arabidopsis thaliana

substrate_name: 2-Oxoglutarate; L-Methionine

sequence_source: UniProtKB reviewed accession Q9LE06; direct lookup 2026-06-25

proof_lines: recombinant enzyme, pH and temperature not specified in the publication

substrate: 2-Oxoglutarate; L-Methionine


sequence_source: UniProtKB reviewed accession Q9M401; direct lookup 2026-06-25


enzyme_display_name: iron(III) enterobactin esterase

organism: Geobacillus thermodenitrificans

substrate_name: 4-nitrophenyl butyrate

enzyme: iron(III) enterobactin esterase

substrate: 4-nitrophenyl butyrate


substrate_name: 4-nitrophenyl caprylate

substrate: 4-nitrophenyl caprylate


substrate_name: 4-nitrophenyl acetate

substrate: 4-nitrophenyl acetate


organism: Escherichia coli

substrate_name: Hydroxymethylbilane

sequence_source: UniProtKB reviewed accession P09126; direct lookup 2026-06-25

substrate: Hydroxymethylbilane


sequence_source: UniProtKB reviewed accession P10746; direct lookup 2026-06-25


proof_lines: wild type enzyme, pH and temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed accession P10746; direct lookup 2026-06-25 and applied mutation signature C73R/A69E to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme C73R/A69E, pH and temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed accession P10746; direct lookup 2026-06-25 and applied mutation signature C73R/L43D to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme C73R/L43D, pH and temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed accession P10746; direct lookup 2026-06-25 and applied mutation signature C73R to reconstruct the assayed variant sequence.

proof_lines: Km above 10 mM, mutant enzyme C73R, pH and temperature not specified in the publication

proof_lines: kcat/Km less than 1 1/sec*mM, mutant enzyme C73R, pH and temperature not specified in the publication


proof_lines: 7-oxocholestanol formation, pH 7.4, 30°C


organism: Klebsiella pneumoniae

assay_conditions_summary: pH 7.4; 310.15 K; buffer phosphate

proof_lines: recombinant enzyme, with L-tyrosine as cosubstrate, in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5-phosphate, at 37°C

proof_lines: recombinant enzyme, in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5-phosphate, at 37°C


substrate_name: L-leucine

proof_lines: apparent Km value, with L-leucine as cosubstrate, at pH 7.4, 37°C

proof_lines: Ki above 0.2 mM, uncompetitive inhibition, at pH 7.4, 37°C

substrate: L-leucine


organism: Bacillus subtilis


organism: Euglena gracilis


organism: Homo sapiens

proof_lines: pH not specified in the publication, temperature not specified in the publication


proof_lines: kcat is 3.7 min(-1) with 5alpha-cholest-7-en-3beta-ol (lathosterol) as substrate. kcat is 2.2 min(-1) with cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol) as substrate.


organism: Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

substrate_name: MGE

proof_lines: kcat is 9 min(-1) with Fe-Ent as substrate. kcat is 850 min(-1) with MGE as substrate. kcat is 1.4 min(-1) with Fe-MGE as substrate.

substrate: MGE


organism: Sinorhizobium sp

substrate_name: S-adenosyl-L-methionine


organism: Rattus norvegicus

proof_lines: inhibition of the last step in the synthesis of phosphatidylcholine


organism: Saccharomyces cerevisiae

substrate_name: phosphatidyl-N-dimethylethanolamine

proof_lines: phosphatidyl-N-dimethylethanolamine as substrate, noncompetitive inhibition

proof_lines: phosphatidyl-N-monomethylethanolamine as substrate, noncompetitive inhibition

proof_lines: phosphatidyl-N-monomethylethanolamine as substrate, competitive inhibition

substrate: phosphatidyl-N-dimethylethanolamine


substrate_name: diglucosyl-enterobactin

substrate: diglucosyl-enterobactin


organism: Methanobacterium ivanovii

substrate_name: uroporphyrinogen III

substrate: uroporphyrinogen III


organism: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

substrate_name: S-adenosyl-L-methionine (in presence of phosphatidyl-N-methylethanolamine (PMME) as substrate)


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: S-adenosyl-L-methionine (in presence of phosphatidylethanolamine (PE) as substrate)


organism: Staphylococcus aureus

assay_conditions_summary: pH 8

proof_lines: pH 8.0, temperature not specified in the publication


substrate_name: palmitoyl-CoA (in the presence of 1-palmitoyl-LPC as cosubstrate)

assay_conditions_summary: cofactor CoA


substrate_name: lysophosphatidylethanolamine

substrate: lysophosphatidylethanolamine


organism: Mus musculus


substrate_name: acetyl-CoA

substrate: acetyl-CoA


organism: Nitratidesulfovibrio vulgaris


sequence_source: UniProtKB reviewed Q3TFD2 / PCAT1_MOUSE, sequence version 1


proof_lines: total membrane fractions of mesangial cells


organism: Oryctolagus cuniculus

proof_lines:
microsomal fraction
neuronal nuclear fraction


substrate_name: hexanoyl-CoA

proof_lines: Km is induced to 0.118 mM in presence of Ca2+

substrate: hexanoyl-CoA


organism: Sus scrofa


enzyme_display_name: procollagen galactosyltransferase

substrate_name: UDP-galactose

enzyme: procollagen galactosyltransferase

substrate: UDP-galactose


substrate_name: UDP-galactose (at 37 degrees Celsius and pH 7.4)


enzyme_display_name: ribosylnicotinamide kinase

organism: Escherichia coli (strain K12)

substrate_name: NMN

enzyme: ribosylnicotinamide kinase

substrate: NMN


organism: Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)


substrate_name: nicotinamide riboside (with ATP as cosubstrate)


substrate: nicotinamide riboside (with ATP as cosubstrate)


organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

substrate_name: ribosylnicotinamide

substrate: ribosylnicotinamide


substrate_name: 5-Hydroxy-L-lysine; UDPgalactose

sequence_source: UniProtKB Q8NBJ5 direct lookup

substrate: 5-Hydroxy-L-lysine; UDPgalactose


substrate_name: UDPgalactose; 5-Hydroxy-L-lysine

verification_status: verified

sequence_source: UniProtKB Q8IYK4 direct lookup

substrate: UDPgalactose; 5-Hydroxy-L-lysine

status: verified


organism: Amphimedon queenslandica

substrate_name: Collagen

substrate: Collagen


organism: Gallus gallus

substrate_name: UDPgalactose

substrate: UDPgalactose


organism: Homo sapiens

proof_lines: value based on actual amount of galactosylhydroxylysyl-residues in ichthyocol

proof_lines: value based on actual amount of galactosylhydroxylysyl-residues in calf skin collagen


organism: Haemophilus influenzae

substrate_name: nicotinamide mononucleotide

proof_lines: pH 7.5, 37°C, nicotinamide mononucleotide adenylyltransferase activity

substrate: nicotinamide mononucleotide


sequence_source: UniProtKB reviewed accession Q9NWW6 direct REST lookup

proof_lines: pH and temperature not specified in the publication, with GTP

proof_lines: pH and temperature not specified in the publication, with ATP


sequence_source: UniProtKB reviewed accession Q9NPI5 direct REST lookup

proof_lines: with GTP, pH and temperature not specified in the publication

proof_lines: with ATP, pH and temperature not specified in the publication


organism: Salmonella enterica

substrate_name: N-Ribosylnicotinamide; ATP

sequence_source: UniProtKB reviewed accession P24518 direct REST lookup

substrate: N-Ribosylnicotinamide; ATP


substrate_name: ATP; N-Ribosylnicotinamide

proof_lines: SABIO-RK modifier species: ribosylnicotinamide kinase(Enzyme) mutant (R212C) N-terminal His-tag

substrate: ATP; N-Ribosylnicotinamide


substrate_name: ATP

sequence_source: UniProtKB reviewed accession P24518 (NADR_SALTY), matched to PMID 15805524 paper strain/gene context.

assay_conditions_summary: pH 7.6; 310.15 K; cofactor ATP

substrate: ATP


substrate_name: phosphate

substrate: phosphate


enzyme_display_name: D-aminoacyl-tRNA deacylase

organism: Arabidopsis thaliana

substrate_name: H2O; D-Tyrosyl-tRNA(Tyr)

sequence_source: UniProtKB REST direct lookup Q9ZPQ3 (UniProtKB reviewed (Swiss-Prot)); accessed 2026-06-25

enzyme: D-aminoacyl-tRNA deacylase

substrate: H2O; D-Tyrosyl-tRNA(Tyr)


organism: Escherichia coli

substrate_name: D-Tyrosyl-tRNA(Tyr); H2O

sequence_source: UniProtKB REST direct lookup P0A6M4 (UniProtKB reviewed (Swiss-Prot)); accessed 2026-06-25

substrate: D-Tyrosyl-tRNA(Tyr); H2O


organism: Pyrococcus abyssi

sequence_source: UniProtKB REST direct lookup Q9V2R8 (UniProtKB reviewed (Swiss-Prot)); accessed 2026-06-25


organism: Synechocystis sp.

substrate_name: D-tyrosyl-tRNATyr

sequence_source: UniProtKB REST direct lookup P73335 (UniProtKB reviewed (Swiss-Prot)); accessed 2026-06-25

substrate: D-tyrosyl-tRNATyr


organism: Thermus thermophilus

substrate_name: D-Tyr-tRNATyr

sequence_source: UniProtKB REST direct lookup Q72K35 (UniProtKB reviewed (Swiss-Prot)); accessed 2026-06-25

substrate: D-Tyr-tRNATyr


sequence_variant_note: Resolved canonical sequence from UniProtKB REST direct lookup Q72K35 (UniProtKB reviewed (Swiss-Prot)); accessed 2026-06-25 and applied mutation signature Y125F to reconstruct the assayed variant sequence.


enzyme_display_name: glycoprotein endo-alpha-1,2-mannosidase

organism: Bacteroides thetaiotaomicron

sequence_variant_note: Resolved canonical sequence from UniProtKB REST direct lookup Q8A109 (UniProtKB unreviewed (TrEMBL)); accessed 2026-06-25 and applied mutation signature E332A to reconstruct the assayed variant sequence.

enzyme: glycoprotein endo-alpha-1,2-mannosidase


sequence_source: UniProtKB REST direct lookup Q8A109 (UniProtKB unreviewed (TrEMBL)); accessed 2026-06-25


source_db: skid

source_license: CC BY-NC-ND 4.0

source: skid


organism: Bacteroides xylanisolvens

sequence_source: UniProtKB REST direct lookup D6D1V7 (UniProtKB unreviewed (TrEMBL)); accessed 2026-06-25


enzyme_display_name: diaminobutyrate decarboxylase

organism: Trichormus variabilis

substrate_name: L-lysine

sequence_source: UniProtKB REST direct lookup Q3M985 (UniProtKB unreviewed (TrEMBL)); accessed 2026-06-25

enzyme: diaminobutyrate decarboxylase

substrate: L-lysine


substrate_name: D-tyrosyl-tRNA(Tyr)

substrate: D-tyrosyl-tRNA(Tyr)


proof_lines: kcat/KM is 6 uM(-1)sec(-1) for D-tyrosyl-tRNA(Tyr) (PubMed:10383414). kcat/KM is 2.8 uM(-1)sec(-1) for D-tryptophanyl-tRNA(Trp) (PubMed:10918062). kcat/KM is 12 uM(-1)sec(-1) for D-aspartyl-tRNA(Asp) (PubMed:10918062). kcat/KM is 10 uM (-1)


organism: Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)


proof_lines: wild type enzyme, in 25 mM HEPES pH 7.0, 100 mM NaCl, at 37°C


organism: Acinetobacter calcoaceticus


organism: Klebsiella aerogenes


organism: Serratia marcescens


organism: Vibrio alginolyticus


organism: Vibrio cholerae


organism: Vibrio cholerae serotype O1

proof_lines:
DABA AT//DC fusion protein
DABA AT/DC fusion protein


enzyme_display_name: carbonic anhydrase

organism: Stylophora pistillata

sequence_source: UniProtKB B5SU02 REST direct lookup, 2026-06-25

assay_conditions_summary: pH 7.5; 293.15 K; buffer but no exact kinetic metric/table value

proof_lines: at 20°C and pH 7.5 in 10 mM HEPES buffer

enzyme: carbonic anhydrase


organism: Methanosarcina thermophila

sequence_variant_note: Resolved canonical sequence from UniProtKB:P40881 with PMID 10924116 mature-chain E84A reconstruction and applied mutation signature E84A with inferred residue-numbering offset +34 to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 7.5; 298.15 K; buffer the reconstructed sequence. Remaining blocker: selected BRENDA snippets list

proof_lines: pH 7.5, 25°C, variant E84A, 50 mM MOPS-Ac buffer

proof_lines: pH 7.5, 25°C, variant E84A, 50 mM MOPS buffer

proof_lines: pH 7.5, 25°C, variant E84A, 50 mM IMID buffer


organism: Homo sapiens

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

assay_conditions_summary: pH 8.98; buffer for carbonic anhydrase I mutant entries

proof_lines: pH 8.98, TAPS buffer, carbonic anhydrase I mutant V62N and carbonic anhydrase I mutant H67N

proof_lines: pH 8.88, TAPS buffer, carbonic anhydrase I mutant V62N,H67N

proof_lines: pH 8.72, 1,2-dimethyimidazole buffer, carbonic anhydrase I mutant V62N,H67N


enzyme_display_name: beta-lactamase

organism: Psychrobacter immobilis A5

substrate_name: benzylpenicillin

sequence_source: UniProtKB/Swiss-Prot O05465 and NCBI Protein CAA58569.1, linked to PMID 9063463 and EMBL X83586.1

enzyme: beta-lactamase

substrate: benzylpenicillin


enzyme_display_name: beta-glucosidase

organism: Rhizomucor miehei

substrate_name: laminaribiose

sequence_source: UniProtKB direct REST check for A0A0B4RUW2 on 2026-06-25

enzyme: beta-glucosidase

substrate: laminaribiose


organism: Saccharolobus solfataricus

sequence_source: UniProtKB direct REST check for Q97UH8 on 2026-06-25


organism: Spodoptera frugiperda

substrate_name: 4-nitrophenyl beta-D-fucoside

sequence_variant_note: Resolved canonical sequence from UniProtKB direct REST check for O61594 on 2026-06-25 and applied mutation signature E194A to reconstruct the assayed variant sequence.

substrate: 4-nitrophenyl beta-D-fucoside


substrate_name: 4-nitrophenyl beta-D-glucoside

sequence_variant_note: Resolved canonical sequence from UniProtKB direct REST check for O61594 on 2026-06-25 and applied mutation signature E190Q to reconstruct the assayed variant sequence.

substrate: 4-nitrophenyl beta-D-glucoside


sequence_variant_note: Resolved canonical sequence from UniProtKB direct REST check for O61594 on 2026-06-25 and applied mutation signature K201A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB direct REST check for O61594 on 2026-06-25 and applied mutation signature E190A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB direct REST check for O61594 on 2026-06-25 and applied mutation signature K201F to reconstruct the assayed variant sequence.


sequence_source: UniProtKB direct REST check for O61594 on 2026-06-25

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


sequence_variant_note: Resolved canonical sequence from UniProtKB direct REST check for O61594 on 2026-06-25 and applied mutation signature M453A to reconstruct the assayed variant sequence.


organism: Talaromyces purpureogenus

sequence_source: UniProtKB direct REST check for C9E9M9 on 2026-06-25

assay_conditions_summary: pH 5; 338.15 K; buffer 100 mM sodium acetate

proof_lines: in 100 mM sodium acetate buffer, pH 5.0, at 65°C


organism: Thermoascus aurantiacus

sequence_source: UniProtKB direct REST check for Q4U4W7 on 2026-06-25


substrate_name: 4-nitrophenyl beta-D-glucopyranoside

sequence_source: UniProtKB direct REST check for Q0ZUL0 on 2026-06-25

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Thermococcus kodakarensis

substrate_name: 4-nitrophenyl beta-D-mannopyranoside

proof_lines: 80°C, pH is not specified in the publication, mutant enzyme D206N

substrate: 4-nitrophenyl beta-D-mannopyranoside


sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: 80°C, pH is not specified in the publication, wild-type


organism: Thermofilum pendens

sequence_source: UniProtKB direct REST check for A1S0B1 on 2026-06-25


organism: Thermoplasma acidophilum

sequence_source: UniProtKB direct REST check for Q9HIL6 on 2026-06-25

assay_conditions_summary: pH 6; 363.15 K; buffer 100 mM citrate/phosphate

proof_lines: in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C


organism: Thermosphaera aggregans

substrate_name: 2-nitrophenyl beta-D-glucopyranoside

sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9YGA8 direct FASTA lookup, 2026-06-25 and applied mutation signature N62Y/Q63K/L231F/V303A/E386G/A412V to reconstruct the assayed variant sequence.

substrate: 2-nitrophenyl beta-D-glucopyranoside


sequence_source: UniProtKB:Q9YGA8 direct FASTA lookup, 2026-06-25

proof_lines: wild-type, pH 6.5, 65°C, presence of 2 M formate


sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

proof_lines: mutant E387G, pH 6.5, 65°C, presence of 2 M formate


organism: Thermothelomyces thermophilus

sequence_source: UniProtKB:G2QDN2 direct FASTA lookup, 2026-06-25


organism: Thermotoga neapolitana

sequence_variant_note: Resolved canonical sequence from UniProtKB:Q0GC07 direct FASTA lookup, 2026-06-25 and applied mutation signature W243F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q0GC07 direct FASTA lookup, 2026-06-25 and applied mutation signature D58A to reconstruct the assayed variant sequence.

proof_lines:
mutant D58A, pH 5.6, 90°C, 0.1 M NaN3
mutant D58A, pH 5.6, 90°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q0GC07 direct FASTA lookup, 2026-06-25 and applied mutation signature D242G to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:Q0GC07 direct FASTA lookup, 2026-06-25

proof_lines:
wild-type, pH 5.6, 90°C, 0.1 M NaN3
wild-type, pH 5.6, 90°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q0GC07 direct FASTA lookup, 2026-06-25 and applied mutation signature E458G to reconstruct the assayed variant sequence.

proof_lines:
mutant E458G, pH 5.6, 90°C
mutant E458G, pH 5.6, 90°C, 0.1 M NaN3


substrate_name: quercetin 3-O-beta-D-glucopyranoside

sequence_variant_note: Resolved canonical sequence from UniProtKB:B9K7M5 direct FASTA lookup, 2026-06-25 and applied mutation signature N220S to reconstruct the assayed variant sequence.

substrate: quercetin 3-O-beta-D-glucopyranoside


sequence_variant_note: Resolved canonical sequence from UniProtKB:B9K7M5 direct FASTA lookup, 2026-06-25 and applied mutation signature F310L to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B9K7M5 direct FASTA lookup, 2026-06-25 and applied mutation signature F224I to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B9K7M5 direct FASTA lookup, 2026-06-25 and applied mutation signature N221F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B9K7M5 direct FASTA lookup, 2026-06-25 and applied mutation signature F310E to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:B9K7M5 direct FASTA lookup, 2026-06-25


sequence_variant_note: Resolved canonical sequence from UniProtKB:B9K7M5 direct FASTA lookup, 2026-06-25 and applied mutation signature W322A to reconstruct the assayed variant sequence.


organism: Phanerodontia chrysosporium

substrate_name: cellobiose

sequence_source: UniProtKB:Q8TGC6 direct lookup 2026-06-25

substrate: cellobiose


substrate_name: p-nitrophenyl-beta-D-glucose

substrate: p-nitrophenyl-beta-D-glucose


sequence_source: UniProtKB:Q25BW5 direct lookup 2026-06-25

proof_lines: pH 6.5, 30°C, recombinant His-tagged isozyme BGL1A


sequence_source: UniProtKB:Q25BW4 direct lookup 2026-06-25


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB:Q25BW5 direct lookup 2026-06-25 and applied mutation signature M177L to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB:Q25BW5 direct lookup 2026-06-25 and applied mutation signature K253A to reconstruct the assayed variant sequence.


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB:Q25BW5 direct lookup 2026-06-25 and applied mutation signature D229N to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB:Q25BW5 direct lookup 2026-06-25 and applied mutation signature V173C to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB:Q25BW5 direct lookup 2026-06-25 and applied mutation signature H231D to reconstruct the assayed variant sequence.


organism: Phytophthora infestans

sequence_source: UniProtKB:Q9AT27 direct lookup 2026-06-25


organism: Piromyces sp.

substrate_name: p-nitrophenyl-beta-D-glucopyranoside

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

substrate: p-nitrophenyl-beta-D-glucopyranoside


organism: Putranjiva roxburghii

sequence_source: UniProtKB:A0A023MIF8 direct lookup 2026-06-25

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Pyrococcus furiosus

sequence_source: UniProtKB:Q51723 direct lookup 2026-06-25


sequence_source: UniProtKB:E7FHY4 direct lookup 2026-06-25


organism: Rasamsonia emersonii

sequence_source: UniProtKB:Q8TGI8 direct lookup 2026-06-25

proof_lines:
pH 5.0, 37°C
pH 5.0, 72°C, recombinant enzyme
pH 5.0, 72°C


organism: Halothermothrix orenii

sequence_variant_note: Resolved canonical sequence from UniProtKB:B8CYA8 canonical sequence with row mutation Y296F reconstructed on 2026-06-25 and applied mutation signature Y296F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:B8CYA8 and applied mutation signature F417S to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 7; 343.15 K; buffer the coupled glucose oxidase/peroxidase method in 20 mM HEPES

column_label: Table 2 (Kinetic parameters for beta-glucosidase and beta-galactosidase activities). Column 1 is the enzyme form; columns 2-4 are the D-cellobiose block with heads 'k_cat,app (s^-1)', 'K_m (mM)', 'k_cat,app/K_m (mM^-1 s^-1)'; columns 5-7 are the D-lactose block with heads 'k_cat,app (s^-1)', 'K_m (mM)', 'k_cat,app/K_m (s^-1 mM^-1)'; column 8 is the selectivity ratio. Body row 'F417S', D-cellobiose block: k_cat,app = 0.38 ± 0.01; K_m = 11.9 ± 0.9; k_cat,app/K_m = 0.032.

normalized_value_unit_evidence: k_cat: printed Table 2 cell 0.38 ± 0.01 in column 'k_cat,app (s^-1)' of the D-cellobiose block; the value is already s^-1, so no conversion is applied and the paper value is 0.38 s^(-1). K_m: printed Table 2 cell 11.9 ± 0.9 in column 'K_m (mM)' of the D-cellobiose block; already mM, no conversion, paper value 11.9 mM. k_cat/K_m: printed Table 2 cell 0.032 in column 'k_cat,app/K_m (mM^-1 s^-1)'; mM^-1 s^-1 and s^-1 mM^-1 are the same unit, already the export unit, so no conversion and the paper value is 0.032 s^(-1)*mM^(-1). Closure check: 0.38 / 11.9 = 0.0319328 s^-1 mM^-1, which rounds to the printed efficiency cell 0.032 at the precision the table prints, so the printed ratio and the quotient of the printed components agree.

raw_value_unit_evidence: Table 2: Kinetic parameters for β-glucosidase and β-galactosidase activities (Applied microbiology and biotechnology 2016, 100(8):3533-3543), body row 'F417S', D-cellobiose block: column 'k_cat,app (s^-1)' prints 0.38 ± 0.01; column 'K_m (mM)' prints 11.9 ± 0.9; column 'k_cat,app/K_m (mM^-1 s^-1)' prints 0.032.

row_label: Table 2, body row 'F417S' (variant F417S), D-cellobiose block


substrate_name: lactose

sequence_variant_note: Resolved canonical sequence from UniProtKB:B8CYA8 canonical sequence with row mutation N294T reconstructed on 2026-06-25 and applied mutation signature N294T to reconstruct the assayed variant sequence.

substrate: lactose


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:B8CYA8 and applied mutation signature N222F to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic parameters for beta-glucosidase and beta-galactosidase activities). Column 1 is the enzyme form; columns 2-4 are the D-cellobiose block with heads 'k_cat,app (s^-1)', 'K_m (mM)', 'k_cat,app/K_m (mM^-1 s^-1)'; columns 5-7 are the D-lactose block with heads 'k_cat,app (s^-1)', 'K_m (mM)', 'k_cat,app/K_m (s^-1 mM^-1)'; column 8 is the selectivity ratio. Body row 'N222F', D-cellobiose block: k_cat,app = 16.9 ± 1.3; K_m = 93.2 ± 12.6; k_cat,app/K_m = 0.18.

normalized_value_unit_evidence: k_cat: printed Table 2 cell 16.9 ± 1.3 in column 'k_cat,app (s^-1)' of the D-cellobiose block; the value is already s^-1, so no conversion is applied and the paper value is 16.9 s^(-1). K_m: printed Table 2 cell 93.2 ± 12.6 in column 'K_m (mM)' of the D-cellobiose block; already mM, no conversion, paper value 93.2 mM. k_cat/K_m: printed Table 2 cell 0.18 in column 'k_cat,app/K_m (mM^-1 s^-1)'; mM^-1 s^-1 and s^-1 mM^-1 are the same unit, already the export unit, so no conversion and the paper value is 0.18 s^(-1)*mM^(-1). Closure check: 16.9 / 93.2 = 0.18133 s^-1 mM^-1, which rounds to the printed efficiency cell 0.18 at the precision the table prints, so the printed ratio and the quotient of the printed components agree.

raw_value_unit_evidence: Table 2: Kinetic parameters for β-glucosidase and β-galactosidase activities (Applied microbiology and biotechnology 2016, 100(8):3533-3543), body row 'N222F', D-cellobiose block: column 'k_cat,app (s^-1)' prints 16.9 ± 1.3; column 'K_m (mM)' prints 93.2 ± 12.6; column 'k_cat,app/K_m (mM^-1 s^-1)' prints 0.18.

row_label: Table 2, body row 'N222F' (variant N222F), D-cellobiose block


sequence_source: UniProtKB:B8CYA8 direct REST check on 2026-06-25


organism: Homo sapiens

sequence_source: UniProtKB:Q9H227 direct REST check on 2026-06-25


organism: Lamium galeobdolon

substrate_name: oleuropein

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

substrate: oleuropein


organism: Listeria innocua Clip11262

substrate_name: sophorotetraose

sequence_source: UniProtKB:Q92AS9 direct REST check on 2026-06-25

substrate: sophorotetraose


substrate_name: sophorose

sequence_variant_note: Resolved canonical sequence from UniProtKB:Q92AS9 canonical sequence with row mutation R572K reconstructed on 2026-06-25 and applied mutation signature R572K to reconstruct the assayed variant sequence.

substrate: sophorose


organism: Mycothermus thermophilus

sequence_source: UniProtKB:A0A191KCR1 direct REST check on 2026-06-25

proof_lines: pH 5.5, 60°C, with 4-nitrophenyl beta-D-glucopyranoside as substrate, wild-type enzyme


sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.


proof_lines: pH 6.0, 50°C, mutant enzyme I48W/I278F/T484Y


sequence_source: UniProtKB:A0A191KCR2 direct REST check on 2026-06-25

proof_lines: pH 6.0, 50°C, with 4-nitrophenyl beta-D-glucopyranoside as substrat, wild-type enzyme


sequence_source: UniProtKB A0A191KCR0 canonical sequence retrieved by direct accession lookup on 2026-06-25.

proof_lines: pH 5.5, 55°C, with 4-nitrophenyl beta-D-glucopyranoside as substrate


sequence_source: UniProtKB O93784 canonical sequence; source accession A0A076JRL8 is inactive/merged and redirects to O93784 in UniProtKB release 2026_02.


organism: Neotermes koshunensis

sequence_source: UniProtKB Q8T0W7 canonical sequence retrieved by direct accession lookup on 2026-06-25.

proof_lines: pH 5.5, 37°C, recombinant His-tagged enzyme


organism: Neurospora crassa

sequence_source: UniProtKB Q7S3R5 canonical sequence retrieved by direct accession lookup on 2026-06-25.


organism: Oryza sativa

substrate_name: cellotriose

sequence_source: UniProtKB Q75I93 canonical sequence retrieved by direct accession lookup on 2026-06-25.

substrate: cellotriose


sequence_source: UniProtKB Q75I93 canonical sequence; row-specific mutant sequence not accepted without complete mutation-coordinate and kinetic record evidence.


substrate_name: gentiobiose

sequence_source: UniProtKB Q8L7J2 canonical sequence retrieved by direct accession lookup on 2026-06-25.

proof_lines: beta-(1-6)-linked gentiobiose, in 100 mM sodium acetate, pH 5.0, at 30°C

proof_lines: beta-(1-2)-linked sophorose, in 100 mM sodium acetate, pH 5.0, at 30°C

substrate: gentiobiose


sequence_source: UniProtKB Q10CU9 canonical sequence retrieved by direct accession lookup on 2026-06-25.

proof_lines: pH and temperature not specified in the publication


organism: Paenibacillus polymyxa

sequence_source: UniProtKB P22073 canonical sequence retrieved by direct accession lookup on 2026-06-25.


organism: Paenibacillus sp.

sequence_source: UniProtKB Q2WGB4 canonical sequence retrieved by direct accession lookup on 2026-06-25.


organism: Pectobacterium carotovorum

sequence_source: UniProtKB Q6QGY5 canonical sequence retrieved by direct accession lookup on 2026-06-25.


organism: Penicillium brasilianum

sequence_source: UniProtKB A5A4M8 canonical sequence retrieved by direct accession lookup on 2026-06-25.

proof_lines: recombinant enzyme, in 50 mM sodium citrate buffer pH 4.8, at 22°C

proof_lines: recombinant enzyme, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C

proof_lines: recombinant enzyme, using cellobiose as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C


organism: Periconia sp. BCC 2871

sequence_source: UniProtKB A9UIG0 canonical sequence retrieved by direct accession lookup on 2026-06-25.


organism: Gelatoporia subvermispora


organism: Unknown


organism: Aspergillus niger

substrate_name: Unknown

substrate: Unknown


proof_lines: pH 4.5, 25°C, recombinant mutants W49N and W49G


proof_lines: pH 4.5, 25°C, recombinant wild-type enzyme

proof_lines: wild type enzyme, in 0.1 M sodium acetate, pH 4.0, at 40°C

proof_lines: wild type enzyme, in 0.1 M sodium acetate, pH 6.0, at 40°C


proof_lines: mutant enzyme D496A, in 0.1 M sodium acetate, pH 4.0, at 40°C


proof_lines: mutant enzyme D487A, in 0.1 M sodium acetate, pH 4.0, at 40°C


proof_lines: mutant enzyme D501A, in 0.1 M sodium acetate, pH 4.0, at 40°C


substrate_name: 2,4-dinitrophenyl beta-D-glucoside

proof_lines: mutant enzyme E490A, in 0.1 M sodium acetate, pH 6.0, at 40°C

proof_lines: mutant enzyme E490A, in 0.1 M sodium acetate, pH 4.0, at 40°C

substrate: 2,4-dinitrophenyl beta-D-glucoside


proof_lines: mutant enzyme D463A, in 0.1 M sodium acetate, pH 4.0, at 40°C


assay_conditions_summary: pH 4.8; 323.15 K; buffer 50 mM citrate

proof_lines: in 50 mM citrate buffer, pH 4.8, at 50°C


organism: Aspergillus oryzae


organism: Thermotoga neapolitana

sequence_variant_note: Resolved canonical sequence from UniProtKB B9K7M5 canonical sequence with selected mutation N221S applied and applied mutation signature N221S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB B9K7M5 canonical sequence with selected mutation N220F applied and applied mutation signature N220F to reconstruct the assayed variant sequence.


organism: Thermotoga petrophila

sequence_source: UniProtKB A5IL97 canonical sequence from direct REST lookup

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


sequence_source: UniProtKB D2C716 canonical sequence from direct REST lookup


sequence_source: UniProtKB A5IL43 canonical sequence from direct REST lookup

proof_lines:
pH 5.0, 90°C, without Zn2+
pH 5.0, 90°C, 2 mM Zn2+


organism: Thermus thermophilus


organism: Trichoderma reesei

sequence_source: UniProtKB Q7Z9M5 canonical sequence from direct REST lookup


organism: Trichoderma viride

sequence_source: UniProtKB C6GGC9 canonical sequence only; variant sequence not reconstructed

proof_lines: mutant enzyme Q627C/R543H/R646V, at pH 5.0 and 50°C


sequence_source: UniProtKB C6GGC9 canonical sequence from direct REST lookup


organism: uncultured bacterium


organism: uncultured microorganism

substrate_name: 4-nitrophenyl beta-D-fucopyranoside

sequence_source: UniProtKB A0A1E1FFN6 canonical sequence only; variant sequence not reconstructed

proof_lines: mutant N223Q, presence of 500 mM D-glucose, pH 5.5, 75°C

substrate: 4-nitrophenyl beta-D-fucopyranoside


sequence_source: UniProtKB A0A1E1FFN6 canonical sequence from direct REST lookup

proof_lines: wild-type, presence of 500 mM D-glucose, pH 5.5, 75°C


proof_lines: mutant N223Y, presence of 500 mM D-glucose, pH 5.5, 75°C


proof_lines: pH 6.0, 60°C, additive: 25 mM cellobiose

proof_lines: pH 6.0, 60°C, additive: 250 mM D-galactose


organism: uncultured rumen bacterium

sequence_source: UniProtKB D3VYD8 canonical sequence from direct REST lookup

proof_lines: pH 6.0, 50°C, compared to KM-value: 0.03 mM for 4-nitrophenyl-beta-D-xylopyranoside of the beta-xylosidase activity, suggesting that RuBGX1 prefers 4-nitrophenyl-beta-D-xylopyranoside substrate


organism: Volvariella volvacea

substrate_name: 4-nitrophenyl beta-D-glucoside

sequence_source: UniProtKB:Q9C3Z9 REST lookup, sequenceVersion 1

substrate: 4-nitrophenyl beta-D-glucoside


organism: Weissella cibaria

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

sequence_source: UniProtKB:I6TNE2 REST lookup, sequenceVersion 1

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Zea mays

sequence_source: UniProtKB:P49235 REST lookup, sequenceVersion 1

sequence_variant_note: Mutation signature F198V could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature D261N matches the mutant residue at offset +15 and the reference residue at offset +8; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


substrate_name: Dhurrin

sequence_source: UniProtKB:P49235 canonical sequence checked; row-specific variant sequence unresolved

proof_lines: pH 5.8, 37°C, chimeric mutant (Chim16) bearing a C-terminal octapeptide (466-477) exchanged with the homologous octapeptide of Sorghum Dhr1 (with an extra 22 amino acids substitution)

proof_lines: pH 5.8, 37°C, chimeric mutant (Chim21) bearing a C-terminal octapeptide (466-477) exchanged with the homologous octapeptide of Sorghum Dhr1

substrate: Dhurrin


sequence_variant_note: Mutation signature Y473F could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature F205L could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature M263F could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature P377A matches the mutant residue at offset -1 and the reference residue at offset -12; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_variant_note: Mutation signature F466S/A467S could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature F466S could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature F198V/F205L/P377A could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines:
wild-type
pH 5.8, 37°C, wild-type


sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset +12, so it is treated as a source-provided variant sequence.


sequence_variant_note: Mutation signature W373K could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature F193A matches the mutant residue at offset +9 and the reference residue at offset +2; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


organism: Aspergillus saccharolyticus

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: pH and temperature not specified in the publication


organism: Aspergillus terreus


organism: Aspergillus tubingensis


organism: Aspergillus versicolor

proof_lines: free enzyme, at 37°C, pH not specified in the publication

proof_lines: immobilized enzyme, at 37°C, pH not specified in the publication


organism: Aspergillus wentii


organism: Avena sativa


proof_lines:
pH 6.8, isoform AsGlu2
pH 6.8, isoform AsGlu1
pH 6.8, 30°C, hexameric enzyme form


organism: Bacillus sp. ZJ1308


organism: Bacillus subtilis

assay_conditions_summary: pH 5

proof_lines: pH 5.0, temperature not specified in the publication


organism: Badhamia polycephala


organism: Bifidobacterium breve


organism: Bos taurus


organism: Acetivibrio thermocellus

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


organism: Agroathelia rolfsii

proof_lines:
beta-glucosidase 3
beta-glucosidase 1
beta-glucosidase 2


organism: Agrobacterium tumefaciens

proof_lines:
no change for chimeric enzymes
marked increase for chimeric enzyme


organism: Alternaria alternata

substrate_name: cellobiose; 4-nitrophenyl-alpha-D-glucoside; Salicin; Laminarin; 4-nitrophenyl-beta-D-galactoside


organism: Apis mellifera

proof_lines: 35°C, pH 5.0, beta-glucosidase purified from ventriculus

proof_lines: 35°C, pH 5.0, beta-glucosidase purified from honey sac


organism: Aspergillus aculeatus


organism: Aspergillus fischeri


organism: Aspergillus foetidus


organism: Aspergillus fumigatus

substrate_name: 4-nitrophenyl-beta-D-glucopyranoside; cellobiose; gentiobiose; 4-nitrophenyl beta-D-glucopyranoside


organism: Aspergillus japonicus

substrate_name: cellobiose; 4-nitrophenyl-beta-D-glucopyranoside; p-nitrophenyl-beta-D-glucopyranoside; amygdalin; Salicin; 2-Nitrophenyl-beta-D-glucopyranoside; methyl-beta-D-glucoside

proof_lines:
at pH 5.0 and 40°C
30°C, 5.0


organism: Aspergillus niger


organism: Zea mays

sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235; reconstructed F200K variant using PMID 19016858 active-site numbering mapped to the UniProt precursor with offset +59 and applied mutation signature F200K with inferred residue-numbering offset +59 to reconstruct the assayed variant sequence.


organism: Caldicellulosiruptor saccharolyticus

substrate_name: 2-nitrophenyl beta-D-glucopyranoside

substrate: 2-nitrophenyl beta-D-glucopyranoside


organism: [Candida] sake

substrate_name: p-nitrophenyl-beta-D-glucopyranoside


organism: Carica papaya


organism: Cellulomonas biazotea

substrate_name: cellobiose


organism: Cellulomonas flavigena


organism: Cellulomonas gilvus


assay_conditions_summary: pH 6.5; 303.15 K; buffer 50 mM MOPS

proof_lines: wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C


organism: Cerrena unicolor


organism: Chilo suppressalis

assay_conditions_summary: pH 5; 310.15 K; buffer 100 mM universal

proof_lines: enzyme from salivary gland, in 100 mM universal buffer (pH 5.0) at 37°C

proof_lines: enzyme from midgut, in 100 mM universal buffer (pH 5.0) at 37°C


organism: Citrus sinensis

substrate_name: p-nitrophenyl-beta-D-glucopyranose

substrate: p-nitrophenyl-beta-D-glucopyranose


organism: Dalbergia cochinchinensis

proof_lines:
pH 5.0, 30°C, native enzyme
pH 5.0, 30°C, recombinant enzyme


organism: Halalkalibacterium halodurans


organism: Hanseniaspora uvarum


organism: Hanseniaspora vineae

substrate_name: Salicin

substrate: Salicin


organism: Homo sapiens

proof_lines:
37°C, pH 6.5
50°C, pH 6.5
pH 7.0, recombinant enzyme


substrate_name: 4-methylumbelliferyl beta-D-glucoside

proof_lines: pH 5.5, 37°C, mutant N370S/N370S, Gauchers disease patient

proof_lines: pH 5.5, 37°C, mutant N370S and deletion mutation, Gauchers disease patient

proof_lines: pH 5.5, 37°C, mutant N370S, heterozygote

substrate: 4-methylumbelliferyl beta-D-glucoside


proof_lines: pH 5.5, 37°C, mutant L444P, heterozygote


proof_lines: pH 5.5, 37°C, mutant N370S/L444P, Gauchers disease patient


proof_lines: pH 5.5, 37°C, mutant D409H, heterozygote


organism: Hordeum vulgare

substrate_name: sophorose


organism: Humicola sp.

proof_lines:
extracellular enzyme
intracellular enzyme


organism: Jeotgalibacillus malaysiensis


organism: Agrobacterium tumefaciens

proof_lines: mutant H229S, pH not specified in the publication, temperature not specified in the publication

substrate: cellobiose


proof_lines: mutant V176A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant L178A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant C174V, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant L178E, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant W127F, pH not specified in the publication, temperature not specified in the publication


proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication

proof_lines: wild-type, presence of 0.25 M glucose, pH not specified in the publication, temperature not specified in the publication

proof_lines: wild-type, presence of 0.5 M glucose, pH not specified in the publication, temperature not specified in the publication


organism: Anoxybacillus flavithermus subsp. yunnanensis


enzyme_display_name: Beta-glucosidase 14 (AtBGLU14) (EC 3.2.1.21)

organism: Arabidopsis thaliana

sequence_source: UniProtKB direct lookup accession Q9SLA0.

proof_lines: with ortho-nitrophenyl-beta-D-glucopyranoside as substrate

proof_lines: with 4-methylumbelliferyl-beta-D-glucopyranoside as substrate

proof_lines: with para-nitrophenyl-beta-D-glucopyranoside as substrate


enzyme_display_name: Beta-glucosidase 21 (AtBGLU21) (EC 3.2.1.21) (Protein PHOSPHATE STARVATION-RESPONSE 3.2)

substrate_name: scopolin

sequence_source: UniProtKB direct lookup accession Q9C525.

proof_lines:
recombinant enzyme, pH 6, 37°C
native enzyme, pH 6, 37°C

substrate: scopolin


enzyme_display_name: beta-glucosidase (EC 3.2.1.21)

organism: Aspergillus niger

sequence_source: UniProtKB direct lookup accession Q9P8F4.

enzyme: beta-glucosidase (EC 3.2.1.21)


organism: Caldicellulosiruptor saccharolyticus

sequence_source: UniProtKB direct lookup accession A4XIG7.

assay_conditions_summary: pH 5.5; 343.15 K; buffer 50 mM citrate

proof_lines: in 50 mM citrate buffer, at pH 5.5 and 70°C


enzyme_display_name: Ipecoside beta-D-glucosidase IpeGLU1 (EC 3.2.1.220) (Ipecac alkaloid beta-glucosidase 1) (IpeGLU1) (Strictosidine beta-glucosidase IpeGLU1) (EC 3.2.1.105)

organism: Carapichea ipecacuanha

substrate_name: strictosidine

substrate: strictosidine


organism: Coprinopsis cinerea

sequence_source: UniProtKB direct lookup accession A8NIX3.

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Glycine max

substrate_name: daidzein 7'-O-beta-D-glucoside

sequence_source: UniProtKB direct lookup accession Q08IT7.

substrate: daidzein 7'-O-beta-D-glucoside


enzyme_display_name: Beta-glucosidase (EC 3.2.1.21)

organism: Halothermothrix orenii

substrate_name: lactose

sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:B8CYA8 and applied mutation signature F417Y to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic parameters for beta-glucosidase and beta-galactosidase activities). Column 1 is the enzyme form; columns 2-4 are the D-cellobiose block with heads 'k_cat,app (s^-1)', 'K_m (mM)', 'k_cat,app/K_m (mM^-1 s^-1)'; columns 5-7 are the D-lactose block with heads 'k_cat,app (s^-1)', 'K_m (mM)', 'k_cat,app/K_m (s^-1 mM^-1)'; column 8 is the selectivity ratio. Body row 'F417Y', D-lactose block: k_cat,app = 41.5 ± 1.2; K_m = 10.3 ± 0.9; k_cat,app/K_m = 4.0.

normalized_value_unit_evidence: k_cat: printed Table 2 cell 41.5 ± 1.2 in column 'k_cat,app (s^-1)' of the D-lactose block; the value is already s^-1, so no conversion is applied and the paper value is 41.5 s^(-1). K_m: printed Table 2 cell 10.3 ± 0.9 in column 'K_m (mM)' of the D-lactose block; already mM, no conversion, paper value 10.3 mM. k_cat/K_m: printed Table 2 cell 4.0 in column 'k_cat,app/K_m (s^-1 mM^-1)'; mM^-1 s^-1 and s^-1 mM^-1 are the same unit, already the export unit, so no conversion and the paper value is 4.0 s^(-1)*mM^(-1). Closure check: 41.5 / 10.3 = 4.02913 s^-1 mM^-1, which rounds to the printed efficiency cell 4.0 at the precision the table prints, so the printed ratio and the quotient of the printed components agree.

raw_value_unit_evidence: Table 2: Kinetic parameters for β-glucosidase and β-galactosidase activities (Applied microbiology and biotechnology 2016, 100(8):3533-3543), body row 'F417Y', D-lactose block: column 'k_cat,app (s^-1)' prints 41.5 ± 1.2; column 'K_m (mM)' prints 10.3 ± 0.9; column 'k_cat,app/K_m (s^-1 mM^-1)' prints 4.0.

row_label: Table 2, body row 'F417Y' (variant F417Y), D-lactose block

enzyme: Beta-glucosidase (EC 3.2.1.21)

substrate: lactose


organism: Daldinia eschscholtzii

proof_lines: pH and temperature not specified in the publication


organism: Erinnyis ello


organism: Eubacterium sp.

substrate_name: esculin

substrate: esculin


organism: Evernia prunastri


organism: Flavobacterium sp.

substrate_name: Gentiotriose

substrate: Gentiotriose


organism: Fomitopsis palustris

proof_lines: isoform BGL1, pH not specified in the publication, at 55°C


organism: Fusarium oxysporum


organism: Fusarium proliferatum

substrate_name: ginsenoside Rg3

assay_conditions_summary: pH 5; 323.15 K; buffer note in NaAc/HAc

proof_lines: in NaAc/HAc buffer (0.2 M, pH 5.0), at 50°C

substrate: ginsenoside Rg3


organism: Fusarium solani

substrate_name: 4-nitrophenyl beta-D-glucopyranoside


organism: Fusobacterium sp.

substrate: p-nitrophenyl-beta-D-glucopyranoside


organism: Galleria mellonella

proof_lines: in 50 mM sodium acetate, pH 5.5, at 37°C


substrate_name: p-nitrophenyl-alpha-D-mannopyranoside

substrate: p-nitrophenyl-alpha-D-mannopyranoside


organism: Mucor circinelloides


organism: Mycothermus thermophilus

assay_conditions_summary: pH 5; 323.15 K; buffer 50 mM sodium phosphate

proof_lines: at 50°C, in 50 mM sodium phosphate buffer, pH 6.0


organism: Nakazawaea peltata


organism: Nakazawaea wickerhamii


organism: Olea europaea


organism: Orobanche minor


organism: Orpinomyces sp.


organism: Oryza sativa

substrate_name: tuberonic acid beta-D-glucoside

substrate: tuberonic acid beta-D-glucoside


organism: Paecilomyces sp.


organism: Paecilomyces sp. 'thermophila'

substrate: sophorose


organism: Paenibacillus polymyxa


organism: Penicillium aurantiogriseum

substrate_name: amygdalin

proof_lines:
for PGI
for PGII

substrate: amygdalin


organism: Sorghum bicolor

substrate_name: Dhurrin

substrate: Dhurrin


substrate_name: 2-nitrophenyl beta-D-glucopyranoside

substrate: 2-nitrophenyl beta-D-glucopyranoside


organism: Spodoptera frugiperda

substrate_name: p-nitrophenyl-beta-D-galactopyranoside

sequence_source: UniProt O61594 / NCBI AAC06038.1, linked from PMID 11342030.

proof_lines: vs. p-nitrophenyl-beta-D-glucopyranoside,30°C, pH 6.0

substrate: p-nitrophenyl-beta-D-galactopyranoside


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt O61594 / NCBI AAC06038.1 canonical Sfbetagly sequence using the selected PMID 20027479 mutation signature. and applied mutation signature Q39N/E451S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt O61594 / NCBI AAC06038.1 canonical Sfbetagly sequence using the selected PMID 20027479 mutation signature. and applied mutation signature Q39N to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt O61594 / NCBI AAC06038.1 canonical Sfbetagly sequence using the selected PMID 20027479 mutation signature. and applied mutation signature Q39E to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt O61594 / NCBI AAC06038.1 canonical Sfbetagly sequence using the selected PMID 20027479 mutation signature. and applied mutation signature E451A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt O61594 / NCBI AAC06038.1 canonical Sfbetagly sequence using the selected PMID 20027479 mutation signature. and applied mutation signature Q39E/E451S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt O61594 / NCBI AAC06038.1 canonical Sfbetagly sequence using the selected PMID 20027479 mutation signature. and applied mutation signature E451S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt O61594 / NCBI AAC06038.1 canonical Sfbetagly sequence using the selected PMID 20027479 mutation signature. and applied mutation signature Q39E/E451A to reconstruct the assayed variant sequence.


organism: Rhizobium leguminosarum


organism: Rhizomucor miehei


organism: Rhynchophorus palmarum

assay_conditions_summary: pH 5; 310.15 K; buffer 100 mM acetate

proof_lines: in 100 mM acetate buffer, pH 5.0, at 37°C


organism: Rhynchosciara americana


organism: Ruminococcus albus


organism: Saccharolobus solfataricus

substrate_name: daidzin

substrate: daidzin


organism: Saccharomyces cerevisiae


organism: Schwanniomyces etchellsii

substrate_name: p-nitrophenyl-beta-D-glucopyranoside


organism: Schwanniomyces vanrijiae

substrate_name: p-nitro-phenyl-beta-D-glucopyranoside

substrate: p-nitro-phenyl-beta-D-glucopyranoside


organism: Sclerocleista ornata


organism: Sclerotinia sclerotiorum


organism: Scytalidium lignicola


organism: Penicillium citrinum

substrate_name: cellobiose

substrate: cellobiose


organism: Penicillium decumbens


organism: Penicillium occitanis (nom. inval.)


organism: Pholiota adiposa


organism: Phoma sp.


organism: Plumeria obtusa

substrate_name: plumieride coumarate beta-D-glucoside

substrate: plumieride coumarate beta-D-glucoside


organism: Prunus armeniaca

proof_lines:
pH 6.0, 35°C
isoform A, pH 5.0, 50°C
isoform B, pH 5.0, 50°C


organism: Prunus dulcis

proof_lines: enzyme immobilized on polysulphone capillary membranes, at pH 6.5 and 25°C

proof_lines: increased Km if enzyme immobilized on cellulose


organism: Pseudomonas sp.

proof_lines: 40°C, pH 6.0, vs. genistein-7-O-beta-D-glucopyranoside


organism: Pyricularia oryzae

substrate_name: cellotetraose

substrate: cellotetraose


organism: Rasamsonia emersonii

substrate_name: Unknown

substrate: Unknown


organism: Rattus sp.


organism: Thermobifida fusca

substrate_name: D-cellobiose

sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature N439S to reconstruct the assayed variant sequence.

substrate: D-cellobiose


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature L444F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature N317Y/L444F to reconstruct the assayed variant sequence.

proof_lines: pH 7.2, 25°C, recombinant mutant N317Y/L444F


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature N317Y/L444F/A433V to reconstruct the assayed variant sequence.

proof_lines: pH 7.2, 25°C, recombinant mutant N317Y/L444F/A433V


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature N178I to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature A433V to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature N317Y to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature N317Y/N439S to reconstruct the assayed variant sequence.

proof_lines: pH 7.2, 25°C, recombinant mutant N317Y/N439S


sequence_source: UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: pH 7.2, 25°C, recombinant wild-type enzyme


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9LAV5 plus selected BRENDA/PubMed mutation context and applied mutation signature S319C to reconstruct the assayed variant sequence.


organism: Thermochaetoides thermophila

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


organism: Thermoclostridium stercorarium


organism: Kluyveromyces marxianus

substrate_name: Salicin

substrate: Salicin


organism: Komagataella pastoris

substrate_name: 4-methylumbelliferyl beta-D-glucoside

proof_lines: pH 7.3, 40°C, versus substrate 4-nitrophenyl beta-D-glucopyranoside

substrate: 4-methylumbelliferyl beta-D-glucoside


organism: Lentinula edodes

substrate: p-nitrophenyl-beta-D-glucopyranoside


organism: Lenzites trabea


organism: Levilactobacillus brevis

assay_conditions_summary: pH 5.5; 310.15 K; buffer 0.2 mM citrate-phosphate

proof_lines: in 0.2 mM citrate-phosphate buffer, pH 5.5, at 37°C


organism: Linum usitatissimum

substrate_name: linustatin

substrate: linustatin


organism: Lissachatina fulica


organism: Lotus japonicus

substrate_name: rhodiocyanoside A

substrate: rhodiocyanoside A


organism: Manihot esculenta


organism: Melanocarpus sp.


organism: Metschnikowia pulcherrima

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Millerozyma farinosa


organism: Spodoptera frugiperda


organism: Sporotrichum pulverulentum

proof_lines:
for enzyme A
cell wall bound enzyme
for enzyme B


organism: Stachybotrys sp.

proof_lines:
pH 6.0, 50°C
pH 4.8, 50°C
at 50°C and pH 5.0


organism: Stereum hirsutum


organism: Streptomyces sp.


organism: Sus scrofa


organism: Talaromyces funiculosus

proof_lines: pH and temperature not specified in the publication


organism: Talaromyces verruculosus


organism: Termitomyces clypeatus

assay_conditions_summary: pH 5; 318.15 K; buffer 0.1 M NaOAc

proof_lines: purified enzyme, in 0.1 M NaOAc buffer, pH 5.0, at 45°C


organism: Termitomyces sp.


organism: Terrabacter ginsenosidimutans


organism: Thermoascus aurantiacus

proof_lines:
pH 5, 50°C
free enzyme, at pH 5.0 and 37°C
immobilized enzyme, at pH 5.0 and 37°C


organism: Zea mays


organism: Thermomyces dupontii

proof_lines: versus 2-nitrophenyl-beta-D-galactopyranoside, pH 6.5, 40°C


organism: Thermomyces lanuginosus


organism: thermophilic anaerobic bacterium


organism: Thermothelomyces heterothallicus

proof_lines: immobilized enzyme to polyacrylamide gel


organism: Thermothelomyces thermophilus

substrate_name: 4-nitrophenyl beta-D-glucose

proof_lines:
pH 5.4, 50°C
at pH 5.0 and 50°C
at pH 5.0 and 60°C

substrate: 4-nitrophenyl beta-D-glucose


organism: Thermotoga maritima

proof_lines: wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C


proof_lines: chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C

proof_lines: chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C


organism: Thermotoga petrophila


organism: Thermus sp.


organism: Thermus thermophilus


organism: Thielaviopsis paradoxa


organism: Tolypocladium cylindrosporum


organism: Trametes versicolor


organism: Trichocladium griseum


organism: Trichoderma koningii


organism: Trichoderma reesei

assay_conditions_summary: pH 5; 313.15 K; buffer 50 mM citrate

proof_lines:
at pH 5.0 and 40°C
beta-glucosidase II
beta-glucosidase I


organism: Triglochin maritima

substrate_name: triglochinin

proof_lines:
beta-glucosidase B, pH 5, 30°C
beta-glucosidase C, pH 5, 30°C

substrate: triglochinin


organism: Triticum aestivum


organism: unidentified microorganism

substrate_name: polydatin

substrate: polydatin


organism: Vanilla planifolia

substrate_name: glucovanillin

substrate: glucovanillin


organism: Verruciconidia persicina


organism: Wickerhamomyces anomalus


organism: Zea mays

substrate_name: 2-nitrophenyl beta-D-glucopyranoside

substrate: 2-nitrophenyl beta-D-glucopyranoside


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: pH 5.8, 25°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1)

proof_lines: pH 5.8, 37°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1)

proof_lines: pH 5.8, 25°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1)


organism: Homo sapiens

assay_conditions_summary: pH 8.94; buffer row

proof_lines: pH 8.94, 1,2-dimethylimidazole buffer, carbonic anhydrase II mutant N62V


proof_lines: pH 8.94, 1,2-dimethylimidazole buffer, carbonic anhydrase II mutant T200H


assay_conditions_summary: pH 6.28; buffer contexts and no measurement_key or exact kinetic lock

proof_lines: pH 6.28, MES buffer, carbonic anhydrase I mutant V62N,H67N,H200T

proof_lines: pH 8.98, TAPS buffer, carbonic anhydrase I mutant V62N,H67N,H200T

proof_lines: pH 7.23, 1-methylimidazolebuffer, carbonic anhydrase I mutant V62N,H67N,H200T


organism: Methanosarcina thermophila

assay_conditions_summary: pH 7.5; 298.15 K; buffer condition

proof_lines: pH 7.5, 25°C, variant E84A, 50 mM MOPS-Ac buffer

proof_lines: pH 7.5, 25°C, variant E84A, 50 mM MOPS buffer

proof_lines: pH 7.5, 25°C, variant E84A, 50 mM IMID buffer


organism: Populus tremula

assay_conditions_summary: pH 6.6; buffer and pH 8

proof_lines:
pH 6.6, MES buffer
pH 8.8, TAPS buffer


enzyme_display_name: alpha-amino-acid esterase

organism: Acetobacter pasteurianus

substrate_name: amoxicillin

source_license: CC BY 4.0; CC BY-NC-ND 4.0

sequence_source: UniProtKB REST FASTA Q8VRK8; fetched 2026-06-25

proof_lines:
30°C, pH 6.2
Raw SKiD Env: Temp=30°C, pH=6.2
Raw SKiD Env: Temp=30°C, pH=7

source_databases_merged: brenda skid

enzyme: alpha-amino-acid esterase

substrate: amoxicillin


substrate_name: valacyclovir

sequence_source: UniProtKB REST FASTA Q86WA6; fetched 2026-06-25

substrate: valacyclovir


organism: Xanthomonas campestris pv. campestris

sequence_variant_note: Resolved canonical sequence from UniProtKB REST FASTA B0RS62; fetched 2026-06-25 and applied mutation signature N186D/A275P/V622I/E143G to reconstruct the assayed variant sequence.


substrate_name: cefadroxil

sequence_source: UniProtKB REST FASTA B0RS62; fetched 2026-06-25

proof_lines: pH 7.0, 25°C, recombinant wild-type enzyme and mutants N186D/A275P/V622I/E143G and N186D/A275P/V622I/E143H

substrate: cefadroxil


sequence_variant_note: Resolved canonical sequence from UniProtKB REST FASTA B0RS62; fetched 2026-06-25 and applied mutation signature N186D/A275P/V622I/E143H to reconstruct the assayed variant sequence.


substrate_name: cefaloglycin

proof_lines:
pH 6.0, 30°C, recombinant enzyme
30°C, pH 7.0
hydrolysis, pH 6.2, 27-30°C

substrate: cefaloglycin


organism: Bacillus mycoides

substrate_name: phenylalanine methylester

substrate: phenylalanine methylester


proof_lines:
pH 7.4, 37°C, recombinant enzyme
pH 7.4, 37°C


organism: Paracidovorax avenae

substrate_name: D-4-hydroxyphenylglycine methyl ester

substrate: D-4-hydroxyphenylglycine methyl ester


organism: Pseudomonas melanogenum

substrate_name: cefradine

substrate: cefradine


organism: Rattus norvegicus


organism: Xanthomonas campestris

substrate_name: D-phenylglycine methyl ester

substrate: D-phenylglycine methyl ester


organism: Xanthomonas citri

substrate_name: D-phenylglycine amide

proof_lines: hydrolysis, pH 6.4, 25°C, recombinant enzyme

proof_lines: acyl transfer from D-alpha-phenylglycine methyl ester to 7-amino-3-deacetoxycephalosporanic acid

substrate: D-phenylglycine amide


organism: Xanthomonas sp.


enzyme_display_name: alpha-mannosidase

organism: Canavalia ensiformis

substrate_name: p-nitrophenyl-alpha-D-mannoside

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

enzyme: alpha-mannosidase

substrate: p-nitrophenyl-alpha-D-mannoside


substrate_name: alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-D-GlcNAc (at pH 4.0 and 37 degrees Celsius in the presence of 1mM CoCl2)


substrate_name: p-nitrophenyl-alpha-D-mannopyranoside (pNP-Man) (at 37 degrees Celsius and at pH 4.5)


organism: Lablab purpureus

substrate_name: p-nitrophenyl-alpha-D-mannopyranoside

substrate: p-nitrophenyl-alpha-D-mannopyranoside


organism: Trichoderma reesei


organism: Trypanosoma cruzi


organism: Yarrowia lipolytica


organism: Drosophila melanogaster

substrate_name: Unknown

sequence_source: UniProtKB:O97043 direct REST lookup 2026-06-25

assay_conditions_summary: pH 6

substrate: Unknown


organism: Mus musculus

sequence_source: UniProtKB:O09159 direct REST lookup 2026-06-25


organism: Picrophilus oshimae


organism: Saccharolobus solfataricus

substrate_name: 4-nitrophenyl alpha-D-mannoside

sequence_source: UniProtKB:Q97UK5 direct REST lookup 2026-06-25

substrate: 4-nitrophenyl alpha-D-mannoside


proof_lines: pH 5.0, 70°C, enzyme in complex with Co2+

proof_lines: pH 5.0, 70°C, enzyme in complex with Mn2+


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q97UK5 direct REST lookup 2026-06-25 and applied mutation signature H533Q to reconstruct the assayed variant sequence.


proof_lines: pH 5.0, 70°C, 1 mM Cd2+, mutant enzyme H533Q

proof_lines: pH 5.0, 70°C, 0.1 mM Zn2+, mutant enzyme H533Q

proof_lines: pH 5.0, 70°C, 1 mM Zn2+, mutant enzyme H533Q


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q97UK5 direct REST lookup 2026-06-25 and applied mutation signature H533E to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q97UK5 direct REST lookup 2026-06-25 and applied mutation signature H228E to reconstruct the assayed variant sequence.

proof_lines: pH 5.0, 70°C, 1 mM Co2+, mutant enzyme H228E

proof_lines: pH 5.0, 70°C, 1 mM Cd2+, mutant enzyme H228E

proof_lines: pH 5.0, 70°C, 1 mM Zn2+, mutant enzyme H228E


proof_lines: pH 5.0, 70°C, 1 mM Co2+, mutant enzyme H533E

proof_lines: pH 5.0, 70°C, 0.1 mM Zn2+, mutant enzyme H533E. KM is too high (above 10 mM for 4-nitrophenyl alpha-D-mannoside or.0.1 mM for Mn2+) to allow for determination of kinetic parameters other than kcat/KM

proof_lines: pH 5.0, 70°C, 1 mM Mn2+, mutant enzyme H533E. KM is too high (above 10 mM for 4-nitrophenyl alpha-D-mannoside or.0.1 mM for Mn2+) to allow for determination of kinetic parameters other than kcat/KM


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q97UK5 direct REST lookup 2026-06-25 and applied mutation signature H228Q to reconstruct the assayed variant sequence.


proof_lines: pH 5.0, 70°C, 1 mM Co2+, mutant enzyme H228Q

proof_lines: pH 5.0, 70°C, 1 mM Zn2+, mutant enzyme H228Q

proof_lines: pH 5.0, 70°C, 1 mM Mn2+, mutant enzyme H228Q. KM is too high (above 10 mM for 4-nitrophenyl alpha-D-mannoside or.0.1 mM for Mn2+) to allow for determination of kinetic parameters other than kcat/KM


sequence_source: UniProtKB Q97UK5

proof_lines: pH 5.0, 70°C, 1 mM Co2+, wild-type enzyme

proof_lines: pH 5.0, 70°C, 1 mM Cd2+, wild-type enzyme

proof_lines: pH 5.0, 70°C, 1 mM Mn2+, wild-type enzyme


organism: Streptococcus pyogenes M1 GAS

sequence_source: UniProtKB Q99YP5


proof_lines:
pH 6.8, 37°C, recombinant enzyme
pH 6.8, 37°C


organism: Mycobacterium tuberculosis

proof_lines: pH not specified in the publication, at 37°C


organism: Oryctolagus cuniculus


organism: Oryza sativa


organism: Phaseolus vulgaris

proof_lines:
alpha-mannosidase I
alpha-mannosidase II


organism: Prunus dulcis


organism: Prunus serotina


organism: Rattus sp.

proof_lines: unbound isoform from cobalt-chelating Sepharose

proof_lines: Tris-eluted isoform from cobalt-chelating Sepharose


organism: Saccharomyces cerevisiae


organism: Solanum lycopersicum


organism: Streptococcus pneumoniae


organism: Sus scrofa


organism: Thermotoga maritima


organism: Aspergillus fischeri

mutation_signature: reaction stopped with Na2CO3

proof_lines: pH 6.5, 50°C, 15 min, reaction stopped with Na2CO3


proof_lines: low binding up to 40°C, increased binding at 50°C


organism: Aspergillus flavus


organism: Aspergillus niger


organism: Aspergillus oryzae


organism: Aspergillus phoenicis

substrate_name: Mannan

substrate: Mannan


organism: Aspergillus sp.


organism: Canavalia ensiformis

proof_lines:
fairly constant between pH 4.5-6.0
at pH 7.0
at pH 3.5


organism: Canavalia sp.


organism: Candida albicans

proof_lines:
enzyme II
enzyme I


organism: Capsicum annuum


organism: Clostridium perfringens


organism: Drosophila melanogaster

substrate_name: 2,4-dinitrophenyl alpha-D-mannoside

substrate: 2,4-dinitrophenyl alpha-D-mannoside


organism: Equus caballus


organism: Gallus gallus

proof_lines:
pH 7.0, 37°C, Co2+-treated enzyme
pH 7.0, 37°C, not treated with Co(II)


organism: Helianthus annuus

proof_lines:
isoforms I
isoforms II


organism: Homo sapiens

proof_lines:
pH 5.0, 37°C, HCEC cell layer
pH 5.0, 37°C, LNZ-308 cell layer
pH 5.0, 37°C, LN-18 cell layer


organism: Lablab purpureus

substrate_name: 4-nitrophenyl alpha-D-mannopyranoside

sequence_source: UniProtKB reviewed accession C0HJW7; evidence at protein level; cites PMID 23422784.

substrate: 4-nitrophenyl alpha-D-mannopyranoside


organism: Lupinus angustifolius

proof_lines:
alpha-mannosidase B
alpha-mannosidase A


organism: Macaca radiata


organism: Medicago sativa


enzyme_display_name: methyl-branched lipid omega-hydroxylase

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

proof_lines: kcat is 11.7 min(-1) for cholesterol as substrate. kcat is 1.5 min(-1) for cholest-4-en-3-one as substrate.

enzyme: methyl-branched lipid omega-hydroxylase


enzyme_display_name: o-aminophenol oxidase

organism: Streptomyces antibioticus

proof_lines:
free enzyme, at pH 4.5 and 25°C
enzyme on nanogel, at pH 4.5 and 25°C
Raw SKiD Env: Temp=25°C, pH=4.5

enzyme: o-aminophenol oxidase


organism: Bauhinia monandra

substrate_name: o-aminophenol

substrate: o-aminophenol


organism: Streptomyces griseus

sequence_source: UniProtKB:B1VTI5; reviewed; GriF/grixazone synthase/o-aminophenol oxidase; PubMed 16282322 evidence


organism: Trametes coccinea

substrate_name: FMN

assay_conditions_summary: cofactor FMN

substrate: FMN


organism: Streptomyces griseus subsp. griseus (strain JCM 4626 / CBS 651.72 / NBRC 13350 / KCC S-0626 / ISP 5235)

substrate_name: alpha-aminophenol

proof_lines: kcat is 20 sec(-1) with alpha-aminophenol as substrate. kcat is 14 sec(-1) with 3,4-AHBAL as substrate. kcat is 18 sec(-1) with 2-amino-4-methylphenol as substrate. kcat is 0.8 sec(-1) with 3,4-dihydroxybenzaldehyde as substrate. kcat is 12

substrate: alpha-aminophenol


organism: Mycobacterium tuberculosis

substrate_name: geranylgeraniol

sequence_source: UniProtKB:P9WPP3 direct REST lookup on 2026-06-25

proof_lines:
at pH 7.4 and 25°C
Raw SKiD Env: Temp=25°C, pH=7.4

substrate: geranylgeraniol


substrate_name: farnesol

substrate: farnesol


organism: Bacillus cereus

substrate_name: benzylpenicillin

sequence_source: UniProtKB reviewed accession P14488 (BLAB_BACCE) direct REST lookup during worker run

substrate: benzylpenicillin


organism: Bacillus licheniformis

substrate_name: penicillin G

sequence_source: UniProtKB reviewed accession P00808 (BLAC_BACLI) direct REST lookup during worker run

proof_lines:
Raw SKiD Env: Temp=22°C, pH=7
Raw SKiD Env: Temp=-----, pH=-----

substrate: penicillin G


organism: Aeromonas hydrophila

substrate_name: Imipenem

sequence_source: UniProt REST direct check, sp|P26918|BLAB_AERHY, reviewed Swiss-Prot entry

proof_lines:
Raw SKiD Env: Temp=-----, pH=8
Raw SKiD Env: Temp=-----, pH=9
Raw SKiD Env: Temp=30°C, pH=6.5

substrate: Imipenem


organism: Escherichia coli

substrate_name: ceftazidime

sequence_source: UniProtKB:P00811 direct accession lookup, release 2026_02

proof_lines:
pH 6.5, 22°C
Raw SKiD Env: Temp=-----, pH=-----

substrate: ceftazidime


substrate_name: nitrocefin

sequence_source: UniProtKB:P13661 direct accession lookup, release 2026_02

proof_lines: pH 7.2, temperature not specified in the publication, recombinant enzyme

substrate: nitrocefin


organism: Pseudomonas aeruginosa

sequence_source: UniProtKB:P16897 direct REST check (https://rest.uniprot.org/uniprotkb/P16897.json)


organism: Serratia marcescens

sequence_source: UniProtKB:P52699 direct REST check (https://rest.uniprot.org/uniprotkb/P52699.json)

proof_lines:
pH 7.5, 25°C
at 30°C
Raw SKiD Env: Temp=25°C, pH=7.5


organism: Psychrobacter immobilis

substrate_name: ampicillin

assay_conditions_summary: pH 7; 303.15 K; buffer 50 mM sodium phosphate

proof_lines: at 30°C in 50mM sodium phosphate buffer (pH 7.0)

substrate: ampicillin


enzyme_display_name: caffeine synthase

organism: Coffea arabica

substrate_name: Theobromine

enzyme: caffeine synthase

substrate: Theobromine


organism: Theobroma cacao


sequence_source: UniProtKB direct accession lookup Q8H0D3; BRENDA source row supplied the accession


substrate_name: S-adenosyl-L-methionine

sequence_source: UniProtKB direct accession lookup Q8H0D2; BRENDA source rows supplied the accession

substrate: S-adenosyl-L-methionine


organism: Paullinia cupana

sequence_source: UniProtKB direct accession lookup A0A061DEC0; BRENDA source row supplied the accession


organism: Camellia sinensis var. assamica

substrate_name: 1,3,7-trimethyluric acid

proof_lines: kcat is 0.00012 sec(-1) with 1,3,7-trimethyluric acid as substrate.

substrate: 1,3,7-trimethyluric acid


organism: Candida albicans

assay_conditions_summary: pH 8.3; 293.15 K; buffer 20 mM Tris

proof_lines: at 20°C, pH 8.3 in 20 mM Tris buffer and 20 mM NaClO4

proof_lines: CaNce103, at 25°C, pH 8.3 in 20 mM Tris buffer and 20 mM NaClO4, in the presence of 0.01 mM histamine


organism: Homo sapiens

assay_conditions_summary: pH 6.28; buffer conditions

proof_lines: pH 6.28, MES buffer, carbonic anhydrase I mutant V62N

proof_lines: pH 8.95, 1,2-dimethylimidazole buffer, carbonic anhydrase I mutant V62N

proof_lines: pH 8.98, TAPS buffer, carbonic anhydrase I mutant V62N


assay_conditions_summary: pH 8.94; buffer for carbonic anhydrase II mutant N67H

proof_lines: pH 8.94, 1,2-dimethylimidazole buffer, carbonic anhydrase II mutant N67H


organism: Methanosarcina thermophila

assay_conditions_summary: pH 7.5; 298.15 K; buffer 50 mM MOPS

proof_lines: pH 7.5, 25°C, variant E62D, 50 mM MOPS buffer


assay_conditions_summary: pH 7.5; 298.15 K; buffer conditions

proof_lines:
pH 7.5, 25°C, wild-type, D2O
pH 5.7-9.5, 25°C, wild-type
pH 6.5, 25°C, wild-type, D2O


proof_lines: pH 7.5, 25°C, variant E84C, 50 mM MOPS buffer


proof_lines: pH 7.5, 25°C, variant E62A/E84A, 50 mM MOPS buffer


proof_lines: pH 7.5, 25°C, variant E84C and E62A, 50 mM MOPS buffer


proof_lines: pH 7.5, 25°C, variant E62Y, 50 mM MOPS buffer


proof_lines: pH 7.5, 25°C, variant E84H, 50 mM MOPS buffer


organism: Oncorhynchus mykiss

substrate_name: 4-nitrophenyl acetate

assay_conditions_summary: pH 9; 298.15 K; buffer note

proof_lines: in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C

substrate: 4-nitrophenyl acetate


enzyme_display_name: 5-aminolevulinate synthase

organism: Unknown

substrate_name: succinyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: 5-aminolevulinate synthase

substrate: succinyl-CoA


substrate_name: glycine

assay_conditions_summary: pH 7.5; 293.15 K; cofactor CoA

proof_lines: co-substrate: succinyl-CoA, mutant Y428I/R433Q /G434N/E435T/L437N, pH 7.5, 20°C

substrate: glycine


proof_lines: co-substrate: succinyl-CoA, mutant A425G/Y428H/R433H/G434N/E435K/L437K, pH 7.5, 20°C


proof_lines: co-substrate: succinyl-CoA, mutant L437Q, pH 7.5, 20°C


proof_lines: co-substrate: succinyl-CoA, mutant R433K/G434K/E435Q/L437Q, pH 7.5, 20°C


organism: Gallus gallus


organism: Mus musculus

proof_lines:
pH 7.5, 20°C, wild-type
wild-type, pH 7.5, 30°C
pH 7.5, 30°C, wild-type


proof_lines:
pH 7.5, 20°C, mutant 2XALAS
pH 7.5, 30°C, mutant 2XALAS


assay_conditions_summary: pH 7.5; 303.15 K; cofactor CoA

proof_lines: mutant R85L, cosubstrate octanoyl-CoA, pH 7.5, 30°C

proof_lines: mutant R85L, cosubstrate succinyl-CoA, pH 7.5, 30°C


substrate_name: butanoyl-CoA

proof_lines: mutant R85K, cosubstrate succinyl-CoA, pH 7.5, 30°C

proof_lines: mutant R85K, cosubstrate 2-hydroxybutanoyl-CoA, pH 7.5, 30°C

substrate: butanoyl-CoA


proof_lines: mutant cosubstrate 2-hydroxybutanoyl-CoA, R85L/T430V, pH 7.5, 30°C

proof_lines: mutant cosubstrate succinyl-CoA, R85L/T430V, pH 7.5, 30°C


organism: Caulobacter vibrioides

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

assay_conditions_summary: pH 7.5; 298.15 K; cofactor CoA


proof_lines: mutant enzyme H340A/C398A, at pH 7.5 and 25°C


sequence_source: UniProtKB:Q9A8J8 direct accession lookup


organism: Cereibacter sphaeroides

sequence_source: UniProtKB:Q04512 direct accession lookup

proof_lines: isoform HemA, pH and temperature not specified in the publication


assay_conditions_summary: pH 7.4; 310.15 K; cofactor CoA

proof_lines: pH 7.4, 37°C, recombinant mutant DELTAAT


substrate_name: Unknown

sequence_source: UniProtKB:P22557 direct accession lookup

proof_lines: apparent value, pH and temperature not specified in the publication

substrate: Unknown


proof_lines: His-tagged mutant enzyme R511E, pH 7.4, 37°C


proof_lines: His-tagged wild-type enzyme, pH 7.4, 37°C

proof_lines: pH 7.4, 37°C, recombinant wild-type enzyme


assay_conditions_summary: pH 7.5; 291.15 K; cofactor CoA


sequence_source: UniProtKB:P08680 direct accession lookup

proof_lines:
wild-type enzyme, pH 7.5, 18°C
wild type enzyme, at pH 7.5 and 37°C
pH 7.5, 37°C, wild-type enzyme


assay_conditions_summary: pH 7.5; 310.15 K; cofactor CoA


assay_conditions_summary: pH 7.2; 310.15 K; cofactor CoA

proof_lines: mutant enzyme T83S/T363S, at pH 7.2 and 37°C


proof_lines: mutant C281P of isoform HemT, pH and temperature not specified in the publication


proof_lines: isoform HemT, pH and temperature not specified in the publication


organism: Escherichia coli

proof_lines: co-substrate: succinyl-CoA, mutant Y428N/P432N/R433I/G434E/E435K/L437K, pH 7.5, 20°C


proof_lines: co-substrate: succinyl-CoA, mutant N427H, pH 7.5, 20°C


proof_lines: co-substrate: succinyl-CoA, mutant V423L/Y428R/P432E/R433I/G434N/E435Q/L437K, pH 7.5, 20°C


proof_lines: co-substrate: succinyl-CoA, mutant A425T, pH 7.5, 20°C


proof_lines: co-substrate: succinyl-CoA, mutant V423I/A425P/Y428C/P432R/R433K/E435N, pH 7.5, 20°C


proof_lines: wild-type, co-substrate: succinyl-CoA, pH 7.5, 20°C


proof_lines:
pH 7.5, 30°C, ALAS(K313A mutant)/ALAS
pH 7.5, 30°C, ALAS/ALAS (K313A mutant)


proof_lines: recombinant erythroid isoform mutant G142C


proof_lines: recombinant erythroid isoform mutant G144S

proof_lines: recombinant erythroid isoform and mutant G144S


proof_lines: recombinant erythroid isoform mutant G144T


proof_lines: recombinant erythroid isoform mutant G144A


proof_lines: recombinant erythroid isoform mutant R433K


proof_lines: recombinant erythroid isoform mutant R439K


proof_lines: recombinant erythroid isoform mutant R433L


proof_lines:
recombinant erythroid isoform
pH 7.5, 30°C, ALAS/ALAS
pH 7.5, 30°C, ALAS


proof_lines: recombinant erythroid isoform mutant D279E


proof_lines: recombinant erythroid isoform mutant Y121F


organism: Rhodopseudomonas palustris

substrate_name: butyryl-CoA

substrate: butyryl-CoA


proof_lines: pH 7.5, 25°C, mutant enzyme R21P/T82S/N84L


proof_lines: pH 7.5, 25°C, mutant enzyme R21N/N84L/T362V


organism: Saccharomyces cerevisiae


organism: Serratia plymuthica


proof_lines: recombinant erythroid isoform mutant Y121H


organism: Paracoccus denitrificans


organism: Plasmodium falciparum


organism: Rattus norvegicus

proof_lines:
erythroid-specific isoform
papain untreated enzyme
papain treated enzyme


proof_lines: pH 7.5, 25°C, mutant enzyme R21N/T82A/T362V


proof_lines: pH 7.5, 25°C, mutant enzyme R21P/N84L/T362X


proof_lines: pH 7.5, 25°C, mutant enzyme R21P/T82S/N84L/T362V


proof_lines: mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C


sequence_source: UniProtKB:Q8I4X1 direct accession lookup

proof_lines: predicted, pH and temperature not specified in the publication. Important potential lead compounds which can serve as inhibitors of Plasmodium falciparum 5-aminolevulinate synthase are identified using pharmacophore modeling, virtual screen


organism: Rhodobacter capsulatus

sequence_source: UniProtKB:P18079 direct accession lookup


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProtKB:P18079 canonical sequence plus selected mutation signature I325M/V390Y/H391I and applied mutation signature I325M/V390Y/H391I to reconstruct the assayed variant sequence.

proof_lines: pH 7.5, 37°C, mutant enzyme I325M/V390Y/H391I


sequence_source: UniProtKB:Q6RVB3 direct accession lookup

assay_conditions_summary: pH 7.5; cofactor CoA


enzyme_display_name: protein farnesyltransferase

substrate_name: farnesyl diphosphate

proof_lines: in 50 mM Tris-HCl (pH 7.0) containing 10 mM MgCl2, 0.01 mM ZnCl2, and 20 nM enzyme, at 30°C

proof_lines: in 50 mM Tris-HCl (pH 7.0) containing 10 mM MgCl2, 0.01 mM ZnCl2, 20 nM enzyme, and 5 mM DTT, at 30°C

enzyme: protein farnesyltransferase

substrate: farnesyl diphosphate


organism: Aspergillus fumigatus

substrate_name: (2E,6E)-farnesyl diphosphate

substrate: (2E,6E)-farnesyl diphosphate


substrate_name: Thr-Lys-Cys-Val-Ile-Met

substrate: Thr-Lys-Cys-Val-Ile-Met


organism: Homo sapiens

substrate_name: HOM-GPP

substrate: HOM-GPP


substrate_name: dansyl-GCVKS

substrate: dansyl-GCVKS


substrate_name: dansyl-GCVDS

substrate: dansyl-GCVDS


substrate_name: YRASNRSCAIM

substrate: YRASNRSCAIM


proof_lines:
apparent turnover number
cosubstrate: dansyl-Gly-Cys-Val-Ile-Met
cosubstrate: farnesyl diphosphate


substrate_name: dansyl-GCQL

sequence_source: UniProtKB reviewed Swiss-Prot Q04631/FNTA_RAT direct accession lookup

proof_lines: with (2E,6E,10E)-3,7,11-trimethyl-12-(prop-2-yn-1-yloxy)dodeca-2,6,10-trien-1-yl diphosphate, pH and temperature not specified in the publication

proof_lines: with farnesyl diphosphate, pH and temperature not specified in the publication

substrate: dansyl-GCQL


enzyme_display_name: glycogen phosphorylase

organism: Dictyostelium discoideum

substrate_name: phosphate

proof_lines: isoenzyme b, in the presence of 3 mM 5-AMP

enzyme: glycogen phosphorylase

substrate: phosphate


organism: Locusta migratoria

substrate_name: glycogen

proof_lines:
phosphorylase a and ab
phosphorylase a
phosphorylase ab

substrate: glycogen


organism: Solanum tuberosum

substrate_name: alpha-D-Glucan

proof_lines:
fast isoenzyme, glycogen phosphorolysis
fast isoenzyme, starch phosphorolysis
fast isoenzyme, starch synthesis

substrate: alpha-D-Glucan


organism: Typha latifolia

substrate_name: maltoheptaose

proof_lines:
synthesis, isoenzyme P-2
synthesis, isoenzyme P-1

substrate: maltoheptaose


proof_lines: phosphorolysis synthesis


organism: Ipomoea batatas


organism: Lathyrus oleraceus

proof_lines:
phosphorolysis at pH 7.2
cytoplasmic phosphorylase
chloroplast phosphorylase


organism: Lethenteron camtschaticum

substrate_name: AMP

proof_lines:
phosphorylase b
phosphorylase a
phosphorylase b, pH 6.3

substrate: AMP


organism: Manduca sexta

proof_lines:
phosphorylase ab
phosphorylase a
phosphorylase b


organism: Mizuhopecten yessoensis

substrate_name: alpha-D-glucose 1-phosphate

substrate: alpha-D-glucose 1-phosphate


organism: Musa x paradisiaca

substrate_name: glucose 1-phosphate

proof_lines:
maltose synthesis, phosphorylase C
maltose synthesis, phosphorylase A
starch synthesis, phosphorylase A

substrate: glucose 1-phosphate


organism: Oryctolagus cuniculus

proof_lines:
phosphorylase a
phosphorolysis
phosphorylase ab


organism: Oryza sativa

substrate_name: amylopectin

substrate: amylopectin


organism: Papiliotrema laurentii


organism: Pyrococcus furiosus

substrate_name: maltotriose

proof_lines: 80°C, pH 6.5, direction of glucose 1-phosphate synthesis

substrate: maltotriose


organism: Rattus norvegicus

substrate_name: Unknown

substrate: Unknown


enzyme_display_name: Glycogen phosphorylase

organism: Aquifex aeolicus

substrate_name: maltotetraose

proof_lines: pH 6.5, 37°C, recombinant enzyme, synthesis reaction

proof_lines: pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction

enzyme: Glycogen phosphorylase

substrate: maltotetraose


enzyme_display_name: Alpha-1,4 glucan phosphorylase

organism: Chlamydomonas reinhardtii

proof_lines: presence of 1 mM orthophosphate, pH 7.0, 30°C

enzyme: Alpha-1,4 glucan phosphorylase


organism: Corynebacterium callunae

substrate_name: maltopentaose

assay_conditions_summary: pH 7

substrate: maltopentaose


enzyme_display_name: Glycogen phosphorylase, liver form

organism: Homo sapiens

proof_lines: pH 6.8, temperature not specified in the publication

enzyme: Glycogen phosphorylase, liver form


enzyme_display_name: Glycogen phosphorylase, muscle form

proof_lines: pH and temperature not specified in the publication

enzyme: Glycogen phosphorylase, muscle form


proof_lines: Pho1 with amylopectin as primer in the reaction, pH 6.5, 22°C

proof_lines: Pho1 with short alpha-glucan chain as primer in the reaction, pH 6.5, 22°C


enzyme_display_name: Alpha-glucan phosphorylase, H isozyme

organism: Triticum aestivum

proof_lines: pH 7.5, 30°C, degradation reaction, cytosolic isozyme

proof_lines: pH 7.5, 37°C, synthesis reaction, cytosolic isozyme

enzyme: Alpha-glucan phosphorylase, H isozyme


organism: Sulfurisphaera tokodaii str. 7

proof_lines: pH 6.2, 37°C, synthetic direction, mutant enzyme k308A


proof_lines: pH 6.2, 37°C, synthetic direction, mutant enzyme H185A


proof_lines: pH 6.2, 37°C, synthetic direction, mutant enzyme R303A


substrate_name: starch

proof_lines: pH 6.2, 37°C, phosphorolytic direction, wild-type enzyme

proof_lines: pH 6.2, 37°C, synthetic direction, wild-type enzyme

substrate: starch


organism: Sus scrofa

proof_lines: kidney dephosphophosphorylase, in the presence of 0.2 mg glycogen


organism: Thermus thermophilus

proof_lines:
phosphorolysis, at 30°C
phosphorolysis, at 70°C
synthesis


organism: Voandzeia subterranea

proof_lines: in the presence of 2 mM glucose 1-phosphate

proof_lines: in the presence of 10 mM glucose 1-phosphate


organism: Zea mays

proof_lines:
phosphorylase II
stromal phosphorylase, phosphorolysis
stromal phosphorylase, synthesis


organism: Saccharolobus solfataricus


organism: Saccharomyces cerevisiae

proof_lines:
synthesis
phosphorolysis
unphosphorylated glycogen phosphorylase


organism: Solanum tuberosum


organism: Spinacia oleracea

proof_lines:
cytoplasmic phosphorylase, pH 7.0
chloroplastphosphorylase, pH 8.0
cytoplasmic phosphorylase, pH 8.0


organism: Streptococcus salivarius

substrate_name: KF

proof_lines: glycogen synthesis, in the presence of NaF

substrate: KF


organism: Acetivibrio thermocellus

substrate_name: dextrin DP19

proof_lines:
pH 7.2, 50°C, degradation reaction
pH 6.8, 50°C, synthesis reaction

substrate: dextrin DP19


organism: Agrobacterium tumefaciens


organism: Badhamia polycephala


organism: Chlorella vulgaris

substrate_name: maltodecaheptaose

proof_lines:
phosphorolysis
synthesis
glucan synthesis

substrate: maltodecaheptaose


organism: Clostridium pasteurianum

proof_lines:
glucan synthesis
phosphorolysis of glucan


organism: Corynebacterium callunae

proof_lines: pH 7.0, 30°C, recombinant wild-type enzyme, phosphorolysis

proof_lines: pH 7.0, 30°C, recombinant wild-type enzyme, synthesis

proof_lines: pH 7.0, 30°C, recombinant wild-type enzyme and mutant H334A, phosphorolysis


proof_lines: pH 7.0, 30°C, recombinant mutant H334Q, phosphorolysis

proof_lines: pH 7.0, 30°C, recombinant mutant H334Q, synthesis


proof_lines: pH 7.0, 30°C, recombinant mutants H334A and H334Q, phosphorolysis


proof_lines: pH 7.0, 30°C, recombinant mutant H334N, phosphorolysis

proof_lines: pH 7.0, 30°C, recombinant mutant H334N, synthesis


proof_lines: pH 7.0, 30°C, recombinant mutant H334A, synthesis

proof_lines: pH 7.0, 30°C, recombinant mutant H334A, phosphorolysis


substrate_name: dextrin DP11

proof_lines:
phosphorolysis
synthesis
maltohexaose arsenolysis

substrate: dextrin DP11


organism: Cuscuta reflexa

proof_lines:
at 18.6 mM starch
at 1.0 mM glucose 1-phosphate
at 0.5 mM glucose 1-phosphate


enzyme_display_name: ornithine carbamoyltransferase

substrate_name: L-ornithine

enzyme: ornithine carbamoyltransferase

substrate: L-ornithine


organism: Squalus acanthias


organism: Sus scrofa