substrate_name: alpha-Ketovaline

assay_conditions_summary: pH 8.6; 298.15 K; cofactor NADH

substrate: alpha-Ketovaline


enzyme_display_name: phenylalanine dehydrogenase

organism: Lysinibacillus sphaericus

source_db: oed

ki_display: Not reported

kcat_over_km_display: Not reported

protein_accession_database: UniProt

source_license: CC BY 4.0

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

smiles: CCC(C)C(=O)C(=O)O

enzyme: phenylalanine dehydrogenase

source: oed


organism: Plasmodium falciparum

substrate_name: Unknown

source_db: brenda

kcat_display: Not reported

km_display: Not reported

ki_unit: mM

mutation_type: substitution

substrate: Unknown

source: brenda


substrate_name: crotonoyl-CoA

assay_conditions_summary: pH 7.4; 298.15 K; cofactor CoA

substrate: crotonoyl-CoA


mutation_signature: Unknown

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: pH not specified in the publication, temperature not specified in the publication


assay_conditions_summary: cofactor NAD+

proof_lines: wild-type, substrate NAD+, pH not specified in the publication, temperature not specified in the publication

proof_lines: wild-type, substrate NADH, pH not specified in the publication, temperature not specified in the publication


organism: Pseudomonas aeruginosa

substrate_name: NADH; H+; trans-Dodec-2-enoyl-[acp]

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

assay_conditions_summary: cofactor NADH

substrate: NADH; H+; trans-Dodec-2-enoyl-[acp]


substrate_name: NADH; H+; Crotonoyl-CoA

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

assay_conditions_summary: pH 7; cofactor NADH

proof_lines: pH 7.0, temperature not specified in the publication

substrate: NADH; H+; Crotonoyl-CoA


organism: Staphylococcus aureus

substrate_name: NADH; H+; Crotonyl-CoA

assay_conditions_summary: cofactor NADPH

substrate: NADH; H+; Crotonyl-CoA


organism: Streptococcus pneumoniae

substrate_name: NADH; H+; But-2-enoyl-[acyl-carrier protein]


assay_conditions_summary: cofactor CoA


organism: uncultured bacterium

substrate_name: NADH

assay_conditions_summary: pH 7; 298.15 K; cofactor NADH

substrate: NADH


organism: Thermoactinomyces intermedius

smiles: CCCCC(=O)C(=O)O


substrate_name: NAD+; H2O; L-Phenylalanine

substrate: NAD+; H2O; L-Phenylalanine


substrate_name: L-Isoleucine

substrate: L-Isoleucine


substrate_name: L-Phenylalanine

substrate: L-Phenylalanine


smiles: CSCCC(=O)C(=O)O


substrate_name: L-Norleucine

substrate: L-Norleucine


substrate_name: L-Leucine

substrate: L-Leucine


organism: Rhodococcus sp.

substrate_name: phenylacetone

source_db: skid

source_license: CC BY-NC-ND 4.0

substrate: phenylacetone

source: skid


organism: Rhodococcus sp. (in: high G+C Gram-positive bacteria)

proof_lines: mutant enzyme K66Q/S149G/N262C, at pH 9.6 and 30°C


substrate_name: phenylpyruvate

substrate: phenylpyruvate


substrate_name: L-Ethionine

substrate: L-Ethionine


substrate_name: L-Methionine

substrate: L-Methionine


substrate_name: L-Norvaline

substrate: L-Norvaline


source_license: CC BY 4.0; CC BY-NC-ND 4.0

assay_conditions_summary: pH 5.5; 303.15 K; cofactor NADH

proof_lines:
pH 5.5, 30°C
Raw SKiD Env: Temp=30°C, pH=5.5

source_databases_merged: brenda skid


organism: Vibrio cholerae

substrate: NADH; H+; But-2-enoyl-[acyl-carrier protein]


organism: Xanthomonas oryzae

substrate_name: crotonyl-CoA

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADH

substrate: crotonyl-CoA


proof_lines:
pH 7.5, 25°C
Raw SKiD Env: Temp=25°C, pH=7.5


organism: Yersinia pestis

assay_conditions_summary: pH 8; 303.15 K; cofactor NADH


assay_conditions_summary: pH 8; 303.15 K; cofactor CoA


proof_lines: IC50 above 0.05 mM, wild type enzyme, at pH 8.0 and 30°C


enzyme_display_name: pyruvate kinase

organism: Chlamydia trachomatis

substrate_name: Phosphoenolpyruvate; ADP

enzyme: pyruvate kinase

substrate: Phosphoenolpyruvate; ADP


organism: Corynebacterium glutamicum


organism: Escherichia coli


organism: Homo sapiens

substrate_name: ADP; Phosphoenolpyruvate

substrate: ADP; Phosphoenolpyruvate


organism: Lactococcus lactis subsp. cremoris


organism: Lactococcus lactis subsp. lactis


organism: Mus musculus


organism: Oryctolagus cuniculus

substrate_name: Phosphoenolpyruvate; dADP

assay_conditions_summary: cofactor ADP

substrate: Phosphoenolpyruvate; dADP


organism: Rattus norvegicus


enzyme_display_name: phenylalanine ammonia-lyase

organism: Arabidopsis thaliana

enzyme: phenylalanine ammonia-lyase


substrate_name: L-Phe

substrate: L-Phe


substrate_name: L-tyrosine

substrate: L-tyrosine


substrate_name: L-phenylalanine

substrate: L-phenylalanine


enzyme_display_name: beta-lactamase

organism: Serratia marcescens

substrate_name: H2O; Cephaloridine

enzyme: beta-lactamase

substrate: H2O; Cephaloridine


substrate_name: H2O; Ampicillin

substrate: H2O; Ampicillin


source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

proof_lines:
pH 7.5, 25°C
at 30°C
Raw SKiD Env: Temp=25°C, pH=7.5

source_databases_merged: sabio_rk brenda skid


organism: Staphylococcus aureus


organism: Stenotrophomonas maltophilia

substrate_name: Nitrocefin; H2O

substrate: Nitrocefin; H2O


organism: Treponema pallidum

substrate_name: Penicillin G; H2O

substrate: Penicillin G; H2O


organism: Anthoceros agrestis


substrate_name: L-Phenylalanine

substrate: L-Phenylalanine


organism: Mycobacterium tuberculosis

substrate_name: H2O; Nitrocefin

substrate: H2O; Nitrocefin


substrate_name: H2O; Penicillin G

substrate: H2O; Penicillin G


organism: Nocardia asteroides


organism: Nocardia farcinica

unit: uM/s


organism: Ochrobactrum anthropi


organism: Proteus mirabilis


organism: Providencia stuartii


organism: Pseudomonas aeruginosa

substrate_name: H2O; Cefotaxime

substrate: H2O; Cefotaxime


organism: Serratia fonticola

substrate_name: H2O; Benzylpenicillin

substrate: H2O; Benzylpenicillin


organism: Aeromonas hydrophila


organism: Bacillus cereus


substrate_name: 6-Aminopenicillanate; H2O

substrate: 6-Aminopenicillanate; H2O


source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0


organism: Bacillus licheniformis

substrate_name: H2O; Phenoxymethylpenicillin

proof_lines:
Raw SKiD Env: Temp=22°C, pH=7
Raw SKiD Env: Temp=-----, pH=-----

substrate: H2O; Phenoxymethylpenicillin


organism: Bacteroides fragilis

proof_lines: pH and temperature not specified in the publication


organism: Burkholderia pseudomallei


organism: Citrobacter diversus


organism: Citrobacter freundii


organism: Enterobacter cloacae


organism: Escherichia coli


substrate_name: Benzylpenicillin; H2O

substrate: Benzylpenicillin; H2O


organism: Thermoactinomyces intermedius

substrate_name: L-Methionine

substrate: L-Methionine


substrate_name: L-Leucine

substrate: L-Leucine


substrate_name: L-Ethionine

substrate: L-Ethionine


substrate_name: L-Norvaline

substrate: L-Norvaline


substrate_name: NAD+; H2O; L-Phenylalanine

substrate: NAD+; H2O; L-Phenylalanine


enzyme_display_name: arylamine N-acetyltransferase

organism: Mycobacterium smegmatis

substrate_name: Acetyl-CoA; Isoniazid

enzyme: arylamine N-acetyltransferase

substrate: Acetyl-CoA; Isoniazid


enzyme_display_name: anthranilate phosphoribosyltransferase

organism: Saccharomyces cerevisiae

enzyme: anthranilate phosphoribosyltransferase


organism: Sulfolobus solfataricus


organism: Amycolatopsis methanolica

substrate_name: Phosphoenolpyruvate; ADP

substrate: Phosphoenolpyruvate; ADP


organism: Bos taurus


organism: Saccharolobus solfataricus

substrate_name: anthranilate

substrate: anthranilate


enzyme_display_name: cathepsin B

organism: Homo sapiens

enzyme: cathepsin B


organism: Larimichthys crocea

substrate_name: Z-L-Phe-L-Arg 7-amido-4-methylcoumarin

substrate: Z-L-Phe-L-Arg 7-amido-4-methylcoumarin


organism: Meretrix meretrix


organism: Mus musculus


organism: Naegleria fowleri


organism: Nicotiana benthamiana

substrate_name: Abz-GIVRAK-Dnp

substrate: Abz-GIVRAK-Dnp


organism: Rattus norvegicus


assay_conditions_summary: cofactor ATP


organism: Ricinus communis


assay_conditions_summary: pH 4.5; cofactor ADP


organism: Klebsiella pneumoniae

substrate_name: H2O; Cephalothin

substrate: H2O; Cephalothin


substrate_name: H2O; Imipenem

substrate: H2O; Imipenem


substrate_name: Nitrocefin; H2O

proof_lines:
pH 7.2, 25°C, recombinant enzyme
Raw SKiD Env: Temp=25°C, pH=7.2

substrate: Nitrocefin; H2O


substrate_name: H2O; Nitrocefin

substrate: H2O; Nitrocefin


substrate_name: H2O; Penicillin G

substrate: H2O; Penicillin G


organism: Klebsiella pneumoniae subsp. ozaenae


organism: Kluyvera ascorbata


organism: Moraxella catarrhalis

substrate_name: Amoxicillin; H2O

substrate: Amoxicillin; H2O


organism: Morganella morganii

substrate_name: H2O; Ampicillin

substrate: H2O; Ampicillin


substrate_name: Benzylpenicillin; H2O

substrate: Benzylpenicillin; H2O


organism: Trichomonas vaginalis


organism: Trypanosoma brucei


organism: Ustilago maydis


organism: Zymomonas mobilis


enzyme_display_name: arginine kinase

organism: Cissites cephalotes

substrate_name: L-Arg

assay_conditions_summary: pH 8; 298.15 K; cofactor ATP

proof_lines: in 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, at 25°C

enzyme: arginine kinase

substrate: L-Arg


organism: Daphnia magna

substrate_name: ATP

proof_lines: wild-type enzyme, pH and temperature not specified in the publication

substrate: ATP


proof_lines: mutant enzyme H284A, pH and temperature not specified in the publication


organism: Euphausia superba

substrate_name: L-arginine

assay_conditions_summary: pH 8; 293.15 K; cofactor ATP

substrate: L-arginine


organism: Fenneropenaeus chinensis

substrate_name: L-Arginine; ATP

substrate: L-Arginine; ATP


organism: Frankliniella occidentalis

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP


organism: Haemonchus contortus

proof_lines: pH 7.5, 30°C, recombinant His-tagged enzyme


organism: Nicotiana benthamiana


organism: Rattus norvegicus

substrate_name: Z-Arg-Arg-p-nitroanilide

substrate: Z-Arg-Arg-p-nitroanilide


substrate_name: H2O; Z-Arg-Arg-p-nitroanilide

substrate: H2O; Z-Arg-Arg-p-nitroanilide


organism: Schistosoma mansoni


substrate_name: Z-Arg-Arg-p-nitroanilide; H2O

substrate: Z-Arg-Arg-p-nitroanilide; H2O


organism: Aeromonas hydrophila

proof_lines:
Raw SKiD Env: Temp=-----, pH=8
Raw SKiD Env: Temp=-----, pH=9
Raw SKiD Env: Temp=30°C, pH=6.5


organism: Arabidopsis thaliana

substrate_name: L-tyrosine

substrate: L-tyrosine


substrate_name: L-phenylalanine

substrate: L-phenylalanine


substrate_name: trans-cinnamate

substrate: trans-cinnamate


organism: Bambusa oldhamii

substrate_name: L-Phenylalanine

substrate: L-Phenylalanine


substrate_name: L-Phe

proof_lines: recombinant protein from Escherichia coli, pH 8.5, 37°C

proof_lines: recombinant protein from Pichia pastoris, pH 8.5, 37°C

proof_lines: recombinant enzyme expressed in Escherichia coli

substrate: L-Phe


proof_lines: recombinant enzyme, in 50 mM Tris-HCl, pH 8.5, at 37°C


substrate_name: L-Tyr

substrate: L-Tyr


organism: Jatropha curcas


organism: Kitagawia praeruptora


organism: Lycoris radiata


organism: Nicotiana tabacum


proof_lines: isoform PAL1, at 30°C in 50 mM Tris/HCl, pH 8.5


organism: Limulus polyphemus

substrate_name: L-Arginine phosphate; ADP

substrate: L-Arginine phosphate; ADP


organism: Litopenaeus vannamei

substrate_name: ATP; dTDP

substrate: ATP; dTDP


organism: Myxococcus xanthus

substrate_name: Nomega-phospho-L-Arg

substrate: Nomega-phospho-L-Arg


organism: Toxocara canis

assay_conditions_summary: pH 8; 298.15 K; buffer and temperature description (Tris-HCl; cofactor NADH

proof_lines: in 100 mM Tris-HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, 5 mM NADH, at 25°C


enzyme_display_name: prolyl oligopeptidase

organism: Bos taurus

substrate_name: H2O; Z-Gly-Pro-AMC

enzyme: prolyl oligopeptidase

substrate: H2O; Z-Gly-Pro-AMC


organism: Homo sapiens


enzyme_display_name: oligopeptidase B

organism: Serratia proteamaculans

proof_lines: pH 8.0, 25°C, recombinant protein with a modified hinge region (PSPmod)

proof_lines: pH 8.0, 25°C, bacterial oligopeptidase B from Serratia proteomaculans (PSP)

proof_lines: substrate Nalpha-benzoyl-DL-Arg-4-nitroanilide, pH 8.0, 25°C

enzyme: oligopeptidase B


assay_conditions_summary: pH 5


organism: Capra hircus

substrate_name: Unknown

proof_lines:
pH 6, 37°C
pH 5, 37°C

substrate: Unknown


organism: Fasciola gigantica


proof_lines:
pH 6.0, 37°C, recombinant enzyme
pH 6.2, 25°C
200 mM NaCl, 2.5 mM DTT, pH 4.5, 37°C


organism: Escherichia coli

substrate_name: Penicillin G; H2O

substrate: Penicillin G; H2O


substrate_name: H2O; Benzylpenicillin

substrate: H2O; Benzylpenicillin


substrate_name: H2O; Penicillin G

substrate: H2O; Penicillin G


substrate_name: H2O; Nitrocefin

proof_lines: pH 7.2, temperature not specified in the publication, recombinant enzyme

substrate: H2O; Nitrocefin


substrate_name: CENTA; H2O

substrate: CENTA; H2O


substrate_name: H2O; Cephaloridine

proof_lines:
pH 6.5, 22°C
Raw SKiD Env: Temp=-----, pH=-----

substrate: H2O; Cephaloridine


substrate_name: H2O; Cefuroxime

substrate: H2O; Cefuroxime


substrate_name: H2O; Ampicillin

proof_lines:
pH 7.5, 25°C
Raw SKiD Env: Temp=25°C, pH=7.5

substrate: H2O; Ampicillin


organism: Fluoribacter gormanii


substrate_name: H2O; Cefotaxime

substrate: H2O; Cefotaxime


substrate_name: H2O; Imipenem

substrate: H2O; Imipenem


organism: Klebsiella oxytoca


organism: Bambusa oldhamii

substrate_name: L-Phe

substrate: L-Phe


substrate_name: L-Tyr

substrate: L-Tyr


organism: Cistanche deserticola


organism: Ephedra sinica

substrate_name: L-phenylalanine

proof_lines:
pH 8.5, 37°C, isozyme PAL2
pH 8.5, 37°C, isozyme PAL1
pH 8.5, 37°C, isozyme PAL3

substrate: L-phenylalanine


organism: Glycine max

substrate_name: L-Phenylalanine

substrate: L-Phenylalanine


organism: Gossypium hirsutum


organism: Nicotiana tabacum


organism: Nostoc punctiforme


assay_conditions_summary: pH 8.5; 303.15 K; buffer a stated assay temperature and Tris/HCl

proof_lines: isoform PAL2, at 30°C in 50 mM Tris/HCl, pH 8.5


proof_lines: isoform PAL4, at 30°C in 50 mM Tris/HCl, pH 8.5


assay_conditions_summary: pH 8.5; 303.15 K; buffer Tris/HCl

proof_lines: isoform PAL3, at 30°C in 50 mM Tris/HCl, pH 8.5


organism: Petroselinum crispum


substrate_name: trans-cinnamate

assay_conditions_summary: pH 10; 303.15 K; buffer E. coli cells as whole cell biocatalysts in ammonium carbonate

substrate: trans-cinnamate


proof_lines:
pH 8.8, 30°C
pH 10, 30°C
Raw SKiD Env: Temp=30°C, pH=8.8


proof_lines: pH and temperature not specified in the publication, mutant enzyme I460V


proof_lines: pH and temperature not specified in the publication, mutant enzyme I460T


proof_lines: pH and temperature not specified in the publication, wild-type enzyme


organism: Rhodobacter sphaeroides


enzyme_display_name: glutamine synthetase

organism: Anabaena azollae

substrate_name: ATP

enzyme: glutamine synthetase

substrate: ATP


substrate_name: L-Glutamate

substrate: L-Glutamate


organism: Arabidopsis thaliana

substrate_name: L-glutamate

assay_conditions_summary: pH 7.8; 303.15 K; cofactor ATP

proof_lines:
pH 7.8, 30°C, isoenzyme GLN1,4
pH 7.8, 30°C, below, isoenzyme GLN1,4

substrate: L-glutamate


proof_lines:
pH 7.8, 30°C, below, isoenzyme GLN1,1
pH 7.8, 30°C, isoenzyme GLN1,1


substrate_name: ATP; L-Glutamate; NH3

substrate: ATP; L-Glutamate; NH3


organism: Exiguobacterium sp.

substrate_name: L-glutamine

proof_lines:
pH 6.0, 35°C, recombinant mutant E60A
pH 6.0, 35°C, recombinant E60A

substrate: L-glutamine


proof_lines:
pH 6.0, 35°C, recombinant mutant R64G
pH 6.0, 35°C, recombinant R64G


substrate_name: ADP

substrate: ADP


proof_lines: pH 6.0, 35°C, recombinant wild-type enzyme


organism: Homo sapiens

substrate_name: L-Glutamine; ADP; Phosphate

substrate: L-Glutamine; ADP; Phosphate


organism: Hordeum vulgare

substrate_name: Unknown

substrate: Unknown


organism: Drosophila melanogaster


organism: Escherichia coli

substrate_name: L-Glutamate; NH3; ATP

substrate: L-Glutamate; NH3; ATP


smiles: N


assay_conditions_summary: pH 7.5; 298.15 K; cofactor ATP


assay_conditions_summary: pH 6; 308.15 K; cofactor ADP


proof_lines: pH 6.0, 35°C, recombinant mutant E60A/R64G


substrate_name: L-Glu

substrate: L-Glu


proof_lines:
isoenzyme GLN1,3
pH 7.8, 30°C, isoenzyme GLN1,3
pH 7.8, 30°C, below, isoenzyme GLN1,3


organism: Rhodotorula glutinis


organism: Rhodotorula graminis

substrate_name: (2E)-3-nitro-cinnamic acid

substrate: (2E)-3-nitro-cinnamic acid


substrate_name: (2E)-3-bromo-cinnamic acid

substrate: (2E)-3-bromo-cinnamic acid


organism: Sorghum bicolor


organism: Trichormus variabilis

proof_lines: mutant Q292C/C565S, pH and temperature not specified in the publication


proof_lines: wild-type, pH and temperature not specified in the publication


substrate_name: (2S)-2-amino-3-phenylpropanoic acid

substrate: (2S)-2-amino-3-phenylpropanoic acid


proof_lines:
pH 8.3, 30°C
Raw SKiD Env: Temp=30°C, pH=8.3
Raw SKiD Env: Temp=-----, pH=-----


organism: Zea mays


organism: Trichormus azollae

assay_conditions_summary: pH 7; 303.15 K; cofactor ATP

proof_lines: D51E mutant enzyme, biosynthetic reaction, pH 7.0, 30°C


organism: Trypanosoma cruzi

proof_lines: recombinant enzyme, coupled reactions assay, pH and temperature not specified in the publication

proof_lines: recombinant enzyme, Malachite green assay, pH and temperature not specified in the publication

proof_lines: recombinant enzyme, pH and temperature not specified in the publication


organism: Tuber borchii

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP


organism: Bacillus subtilis

assay_conditions_summary: pH 7; 298.15 K; cofactor ATP

proof_lines:
Raw SKiD Env: Temp=25°C, pH=-----
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=-----, pH=7


assay_conditions_summary: pH 7; cofactor ATP

proof_lines: pH 7.0, temperature not specified in the publication, wild-type enzyme


proof_lines: pH 7.0, temperature not specified in the publication, mutant E304A


substrate_name: hydroxylamine

proof_lines: pH 7.0, temperature not specified in the publication, mutant R62A

substrate: hydroxylamine


assay_conditions_summary: cofactor ADP


organism: Camellia sinensis

assay_conditions_summary: pH 7.8; cofactor ATP

proof_lines:
pH 7.8
substrate NH4+, pH 7.8
substrate L-glutamate, pH 7.8


organism: Canis familiaris

assay_conditions_summary: cofactor ATP


organism: Pseudomonas taetrolens


organism: Pyrococcus sp.


assay_conditions_summary: pH 7.8; 333.15 K; cofactor ATP


organism: Rattus norvegicus


organism: Ruminococcus albus

assay_conditions_summary: pH 6.4; 310.15 K; cofactor ATP


organism: Salmonella enterica subsp. enterica serovar Typhimurium


organism: Sulfolobus acidocaldarius

assay_conditions_summary: 353.15 K; cofactor ADP


proof_lines: wild-type enzyme, biosynthetic reaction, pH 7.0, 30°C


organism: Leishmania donovani


organism: Medicago truncatula


proof_lines: pH not specified in the publication, 30°C, wild-type mature enzyme


proof_lines: pH not specified in the publication, 30°C, mutant mature enzyme lacking the C-terminal peptide


organism: Methanocaldococcus jannaschii


organism: Mus musculus


organism: Mycobacterium tuberculosis

assay_conditions_summary: pH 7.5; cofactor ATP

proof_lines:
pH 7.5
Raw SKiD Env: Temp=-----, pH=7.5


organism: Oryza sativa


organism: Oryza sativa Japonica Group

assay_conditions_summary: 303.15 K; cofactor ATP


organism: Phaseolus vulgaris


organism: Lysinibacillus sphaericus

substrate_name: DL-propargylglycine

substrate: DL-propargylglycine


substrate_name: L-phenylalanine

substrate: L-phenylalanine


enzyme_display_name: shikimate dehydrogenase (NADP+)

organism: Arabidopsis thaliana

substrate_name: NADP+

assay_conditions_summary: pH 7.5; 295.15 K; cofactor NADP+

enzyme: shikimate dehydrogenase (NADP+)

substrate: NADP+


substrate_name: shikimate

substrate: shikimate


proof_lines: pH 7.5, 22°C, wild-type DELTA88DHQ-SDH enzyme variant


proof_lines:
isoenzyme GLN1,4
pH 7.8, 30°C, isoenzyme GLN1,2
pH 7.8, 30°C, below, isoenzyme GLN1,2


enzyme_display_name: peroxidase

organism: Auricularia auricula-judae

substrate_name: 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) (Dye-decolorizing peroxidase AauDyP1, at pH 4.5)

source_db: uniprot

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

enzyme: peroxidase

source: uniprot


organism: Bubalus bubalis

substrate_name: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (at pH 6.0 and 60 degrees Celsius)


organism: Equus caballus

substrate_name: bromide

substrate: bromide


organism: Metapenaeus ensis

substrate_name: ATP (at pH 8.5 and 30 degrees Celsius)

substrate: ATP (at pH 8.5 and 30 degrees Celsius)


organism: Penaeus vannamei

substrate_name: ATP (at pH 8.6 and 30 degrees Celsius)

substrate: ATP (at pH 8.6 and 30 degrees Celsius)


organism: Populus trichocarpa

assay_conditions_summary: pH 8.5; 295.15 K; cofactor NADP+

proof_lines: with NADP+, pH 8.5, 22°C, recombinant His-tagged Poptr1

proof_lines: pH 8.5, 22°C, with NADP+, isozyme Poptr5

proof_lines: with NAD+, pH 8.5, 22°C, recombinant His-tagged Poptr1


organism: Homo sapiens

substrate_name: Acetyl-CoA; 2-Aminofluorene

assay_conditions_summary: pH 7.4; 310.15 K; cofactor CoA

proof_lines: using control cholangiocarcinoma KKU-100 cells after treatment with the mixture of inflammatory cytokines for 48 h, Vmax: 135.7 pmol/min/mg. A significant decrease in Vmax is shown but without affecting Km, implying a change in the amount o

substrate: Acetyl-CoA; 2-Aminofluorene


organism: Bos taurus

substrate_name: Phosphoenolpyruvate; ADP

proof_lines:
M-type isozyme, 25°C, pH 7.0
L-type isozyme, 25°C, pH 7.0

substrate: Phosphoenolpyruvate; ADP


organism: Monoraphidium minutum

substrate_name: IDP

proof_lines:
isozyme PKc
PKc-isozyme
isozyme PKp

substrate: IDP


organism: Rattus norvegicus

assay_conditions_summary: pH 7.1; 310.15 K; cofactor ATP

proof_lines:
apparent value
pH 7.1, 37°C
R-type isozyme, pH 7.4, 37°C


organism: Ricinus communis

assay_conditions_summary: pH 7.9; 298.15 K; cofactor ADP

proof_lines: 2 isozymes with different kinetic mechanisms


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: ADP

assay_conditions_summary: pH 6.8; 303.15 K; cofactor ADP

proof_lines:
enzyme form I
30°C, pH 6.8
isozyme II, 30°C, pH 6.8

substrate: ADP


organism: Spinacia oleracea

proof_lines:
isozyme PKII, pH 7.1, 25°C
isozyme PKI, pH 7.1, 25°C
pH 7.1, 25°C


organism: Sus scrofa

proof_lines:
isoenzyme I
isoenzyme II
isoenzyme I and isoenzyme II


organism: Petroselinum crispum

substrate_name: L-Phenylalanine

proof_lines:
30°C
pH 8.8, 30°C
isoenzyme PAL-4

substrate: L-Phenylalanine


organism: Unknown

substrate_name: L-Phe

substrate: L-Phe


organism: Beta vulgaris

substrate_name: Unknown

substrate: Unknown


organism: Bradyrhizobium japonicum

substrate_name: hydroxylamine

proof_lines:
second value 8.9 mM, biphasic plot
glutamyl transferase reaction
biosynthetic reaction


organism: Hordeum vulgare


organism: Senna obtusifolia


substrate_name: Glu

proof_lines:
synthetase assay
transferase assay
plastidic isozyme

substrate: Glu


organism: Triticum aestivum

proof_lines: in the presence of 3.5 mM L-glutamate, pH and temperature not specified in the publication

proof_lines: in the presence of 1 mM L-glutamate, pH and temperature not specified in the publication


organism: Xylanibacter ruminicola

substrate_name: ATP

assay_conditions_summary: pH 6; 310.15 K; cofactor ATP

proof_lines: isozyme GSIII-1, pH 6.0, 37°C, gamma-glutamylhydroxamate synthetase activity

proof_lines: isozyme GSIII-2, pH 7.5, 37°C, glutamine synthetase activity

proof_lines: isozyme GSIII-2, pH 6.0, 37°C, gamma-glutamylhydroxamate synthetase activity

substrate: ATP


organism: Zea mays


organism: Arabidopsis thaliana

assay_conditions_summary: 295.15 K; cofactor NADP+


substrate_name: NADP+


substrate_name: shikimate


organism: Lysinibacillus sphaericus

substrate_name: DL-propargylglycine


substrate_name: L-phenylalanine


enzyme_display_name: uronate dehydrogenase

organism: Agrobacterium tumefaciens

substrate_name: D-glucuronic acid

proof_lines: mutant enzyme H99Y/H234K, at pH 8.0 and 30°C

enzyme: uronate dehydrogenase

substrate: D-glucuronic acid


proof_lines: mutant enzyme A39P/H99Y, at pH 8.0 and 30°C


proof_lines: mutant enzyme A39P/H234K, at pH 8.0 and 30°C


proof_lines: mutant enzyme A39P/H99Y/H234K, at pH 8.0 and 30°C


enzyme_display_name: deacetoxycephalosporin-C synthase

organism: Streptomyces clavuligerus

substrate_name: Penicillin N

enzyme: deacetoxycephalosporin-C synthase

substrate: Penicillin N


proof_lines: pH 6.5, 30°C, recombinant mutants C281Y and I305L


substrate_name: penicillin G

substrate: penicillin G


enzyme_display_name: dihydrolipoyl dehydrogenase

organism: Homo sapiens

substrate_name: NAD+

assay_conditions_summary: pH 8; 310.15 K; cofactor NAD+

enzyme: dihydrolipoyl dehydrogenase

substrate: NAD+


substrate_name: dihydrolipoamide

substrate: dihydrolipoamide


smiles: N=C(O)CCCCC(S)CCS


substrate_name: Pyruvate; NAD+; Coenzyme A

assay_conditions_summary: cofactor NAD+

substrate: Pyruvate; NAD+; Coenzyme A


substrate_name: NAD+; 2-Oxoglutarate; Coenzyme A

substrate: NAD+; 2-Oxoglutarate; Coenzyme A


substrate_name: NAD+; Coenzyme A; alpha-Ketovaline

substrate: NAD+; Coenzyme A; alpha-Ketovaline


substrate_name: NAD+; Coenzyme A; 2-Oxoglutarate

substrate: NAD+; Coenzyme A; 2-Oxoglutarate


substrate_name: Lipoamide

substrate: Lipoamide


proof_lines:
mutant enzyme N286Q
mutant N286Q


proof_lines:
mutant enzyme N286D
mutant N286D


proof_lines:
mutant enzyme D320N
mutant D320N


proof_lines:
wild-type enzyme, pH 8.0, 37°C
wild type enzyme, at pH 8.0 and 37°C
wild-type


substrate_name: NADH

assay_conditions_summary: pH 8; 310.15 K; cofactor NADH

substrate: NADH


substrate_name: Dihydrolipoamide; NAD+

substrate: Dihydrolipoamide; NAD+


organism: Solanum tuberosum


substrate_name: 2-Oxoglutarate; NAD+; Coenzyme A

substrate: 2-Oxoglutarate; NAD+; Coenzyme A


substrate_name: 2-Oxopentanoate; NAD+; Coenzyme A

substrate: 2-Oxopentanoate; NAD+; Coenzyme A


substrate_name: NADH; H+; (R)-Lipoate

assay_conditions_summary: cofactor NADH

substrate: NADH; H+; (R)-Lipoate


organism: Thermus scotoductus

substrate_name: Cr(VI)

substrate: Cr(VI)


organism: Xenopus laevis


enzyme_display_name: sulfite oxidase

substrate_name: sulfite

enzyme: sulfite oxidase

substrate: sulfite


organism: Gallus gallus

proof_lines:
wild-type, 25°C, pH 8.5
wild-type, 25°C, pH 7.0


proof_lines:
mutant Y322N/R450M, 25°C, pH 8.5
mutant Y322N/R450M, 25°C, pH 7.0


proof_lines:
Raw SKiD Env: Temp=25°C, pH=8.5
Raw SKiD Env: Temp=25°C, pH=7


substrate_name: Sulfite; Cytochrome c; H2O

substrate: Sulfite; Cytochrome c; H2O


proof_lines: with ferricanide, pH 6.0, 25°C, recombinant wild-type enzyme

proof_lines: with ferricanide, pH 8.9, 25°C, recombinant wild-type enzyme

proof_lines: with ferricanide, pH 7.1, 25°C, recombinant wild-type enzyme


proof_lines: with ferricanide, pH 6.0, 25°C, recombinant mutant C242S/C253S/C260S/C451S

proof_lines: with ferricanide, pH 7.1, 25°C, recombinant mutant C242S/C253S/C260S/C451S

proof_lines: with ferricanide, pH 8.4, 25°C, recombinant mutant C242S/C253S/C260S/C451S


organism: Microbacterium luteolum

assay_conditions_summary: pH 6; 298.15 K; buffer 50 mM potassium phosphate; cofactor NAD+

proof_lines: in the presence of 0.2 mM NAD+, in 50 mM potassium phosphate buffer (pH 6.0), at 25°C


organism: Mycobacterium tuberculosis


substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication

substrate: Unknown


organism: Pisum sativum


substrate_name: Dihydrolipoylprotein

smiles: NC(=O)CCCCC(S)CCS

substrate: Dihydrolipoylprotein


substrate_name: NAD+; Glycine; 5,6,7,8-Tetrahydrofolate

substrate: NAD+; Glycine; 5,6,7,8-Tetrahydrofolate


organism: Rattus norvegicus


organism: Escherichia coli


organism: Glycine max

substrate_name: ferrileghemoglobin

substrate: ferrileghemoglobin


organism: Halobacterium volcanii

substrate_name: NAD+; Dihydrolipoamide

substrate: NAD+; Dihydrolipoamide


organism: Haloferax volcanii

substrate_name: NAD+

substrate: NAD+


substrate_name: dihydrolipoamide

assay_conditions_summary: pH 7; 303.15 K; cofactor NAD+

proof_lines: pH 7.0, 30°C, mutant enzyme E423S, assay in the absence of KCI

substrate: dihydrolipoamide


proof_lines: pH 7.0, 30°C, mutant enzyme E423D, assay in the absence of KCI


proof_lines: pH 7.0, 30°C, mutant enzyme E423A, assay in the absence of KCI


proof_lines: pH 7.0, 30°C, wild-type enzyme, assay in the absence of KCI


proof_lines: pH 7.0, 30°C, mutant enzyme E423Q, assay in the absence of KCI


substrate_name: (S)-Lipoic acid

substrate: (S)-Lipoic acid


substrate_name: (R)-Lipoic acid

substrate: (R)-Lipoic acid


substrate_name: 1,2-Diselenolane-3-pentanoic acid

substrate: 1,2-Diselenolane-3-pentanoic acid


organism: Acidianus ambivalens


organism: Azotobacter vinelandii

substrate_name: Coenzyme A; NAD+; 2-Oxoglutarate

substrate: Coenzyme A; NAD+; 2-Oxoglutarate


organism: Bos taurus

substrate_name: NAD+; Coenzyme A; Pyruvate

substrate: NAD+; Coenzyme A; Pyruvate


organism: Clostridium kluyveri


organism: Corynebacterium glutamicum

substrate_name: NAD+; Coenzyme A; 2-Oxoglutarate

substrate: NAD+; Coenzyme A; 2-Oxoglutarate


substrate_name: Pyruvate; NAD+; Coenzyme A

substrate: Pyruvate; NAD+; Coenzyme A


assay_conditions_summary: pH 7.98; 298.15 K; cofactor NAD+


assay_conditions_summary: pH 7.98; 298.15 K; cofactor NADP+


substrate_name: NADP+

substrate: NADP+


organism: Zea mays

substrate_name: sulfite

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: sulfite


enzyme_display_name: thymidylate synthase

organism: Bacillus subtilis

substrate_name: 5,10-Methylenetetrahydrofolate; dUMP

enzyme: thymidylate synthase

substrate: 5,10-Methylenetetrahydrofolate; dUMP


organism: Bacteriophage T4


organism: Crithidia fasciculata


substrate_name: dUMP

substrate: dUMP


organism: Homo sapiens


proof_lines:
presence of Mg2+, pH 7.5, 22°C
pH 7.5, 22°C
pH 7.2, 22°C


organism: Lactobacillus casei


organism: Leishmania major


assay_conditions_summary: cofactor NADPH


organism: Rattus norvegicus


organism: Toxoplasma gondii

substrate_name: H+; NADPH; 5,10-Methylenetetrahydrofolate; dUMP


organism: Trypanosoma cruzi


enzyme_display_name: glycine hydroxymethyltransferase

organism: Alloalcanivorax dieselolei

substrate_name: L-serine

proof_lines: pH 7.4, 40°C, recombinant wild-type enzyme

enzyme: glycine hydroxymethyltransferase

substrate: L-serine


proof_lines: pH 7.4, 40°C, recombinant mutant E160L/E193Q


organism: Aphanothece halophytica

substrate_name: glycine

proof_lines: pH 7.2, 30°C, recombinant enzyme, in presence of 0.375 M NaCl

substrate: glycine


organism: Arabidopsis thaliana

substrate_name: tetrahydrofolate

proof_lines: pH 8.5, temperature not specified in the publication, recombinant AtSHMT3

substrate: tetrahydrofolate


substrate_name: Tetrahydropteroyl di-L-glutamate

substrate: Tetrahydropteroyl di-L-glutamate


substrate_name: L-Serine; 5,6,7,8-Tetrahydrofolate

substrate: L-Serine; 5,6,7,8-Tetrahydrofolate


substrate_name: Tetrahydropteroyl penta-L-glutamate

substrate: Tetrahydropteroyl penta-L-glutamate


substrate_name: Tetrahydropteroyl tri-L-glutamate

substrate: Tetrahydropteroyl tri-L-glutamate


substrate_name: Tetrahydropteroyl tetra-L-glutamate

substrate: Tetrahydropteroyl tetra-L-glutamate


organism: Bombyx mori


organism: Danio rerio


substrate_name: serine

substrate: serine


organism: Escherichia coli


proof_lines: apparent value, mutant enzyme L276A, at 20°C in 50 mM Na-HEPES (pH 7.2), containing 0.2 mM dithiothreitol and 0.1 mM EDTA


proof_lines: pH 7.2, 30°C, recombinant enzyme, in presence of 0.75 M NaCl

proof_lines: pH 7.2, 30°C, recombinant enzyme, in presence of 0.125 M NaCl

proof_lines: pH 7.2, 30°C, recombinant enzyme, in presence of 0.50 M NaCl


proof_lines: apparent value, mutant enzyme L85A, at 20°C in 50 mM Na-HEPES (pH 7.2), containing 0.2 mM dithiothreitol and 0.1 mM EDTA


proof_lines: apparent value, wild type enzyme, at 20°C in 50 mM Na-HEPES (pH 7.2), containing 0.2 mM dithiothreitol and 0.1 mM EDTA


proof_lines: apparent value, mutant enzyme L85A/L276A, at 20°C in 50 mM Na-HEPES (pH 7.2), containing 0.2 mM dithiothreitol and 0.1 mM EDTA


substrate_name: L-Serine

substrate: L-Serine


organism: Geobacillus stearothermophilus


substrate_name: L-allo-Threonine

substrate: L-allo-Threonine


organism: Glutamicibacter nicotianae


organism: Glycine max

proof_lines: pH 7.5, temperature not specified in the publication, enzyme SHMT8 from Glycine max cv. Essex


substrate_name: D-Serine

substrate: D-Serine


assay_conditions_summary: pH 7; 298.15 K; buffer HEPES

proof_lines: pH 7.0, 25°C, recombinant enzyme, in HEPES buffer

proof_lines: pH 7.0, 25°C, recombinant enzyme, in phosphate buffer


proof_lines:
isoform SHMT2, at pH 7.2 and 20°C
isoform SHMT2, at pH 7.2 and 30°C
isoform SHMT2, at pH 7.8 and 30°C


proof_lines: mutant H135N/R137A/E168N, at pH 7.2 and 20°C


proof_lines: wild type enzyme SHMT1, at pH 7.2 and 20°C


organism: Idiomarina loihiensis

substrate_name: DL-beta-Phenylserine

substrate: DL-beta-Phenylserine


organism: Leishmania donovani


organism: Methanosarcina barkeri

proof_lines: pH and temperature not specified in the publication


organism: Oryctolagus cuniculus

substrate_name: L-Ser

proof_lines: wild-type, in the presence of pyridoxal phosphate

substrate: L-Ser


proof_lines: mutant L474F, in the presence of pyridoxal phosphate


proof_lines: mutant S394N, in the presence of pyridoxal phosphate


organism: Plasmodium vivax


organism: Psychromonas ingrahamii

proof_lines:
pH 7.2, 20°C
Raw SKiD Env: Temp=20°C, pH=7.2


organism: Rattus norvegicus


organism: Ovis aries


organism: Plasmodium falciparum


enzyme_display_name: hypoxanthine phosphoribosyltransferase

organism: Trypanosoma cruzi

substrate_name: IMP

enzyme: hypoxanthine phosphoribosyltransferase

substrate: IMP


substrate_name: diphosphate

substrate: diphosphate


substrate_name: Hypoxanthine

substrate: Hypoxanthine


substrate_name: 5-Phospho-alpha-D-ribose 1-diphosphate

substrate: 5-Phospho-alpha-D-ribose 1-diphosphate


organism: Saccharomyces cerevisiae


organism: Thermoanaerobacter tengcongensis


substrate_name: Guanine

substrate: Guanine


organism: Thermus thermophilus

substrate_name: guanine

substrate: guanine


substrate_name: 5-phospho-alpha-D-ribose 1-diphosphate

substrate: 5-phospho-alpha-D-ribose 1-diphosphate


substrate_name: hypoxanthine

substrate: hypoxanthine


organism: Trypanosoma brucei brucei


proof_lines:
at pH 7.4 and 25°C
Raw SKiD Env: Temp=25°C, pH=7.4


organism: Legionella pneumophila


organism: Leishmania tarentolae

proof_lines: recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate


organism: Mycobacterium tuberculosis


assay_conditions_summary: pH 7.4; 296.15 K; buffer two

proof_lines: activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C

proof_lines: activated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C

proof_lines: activated enzyme, with hypoxanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C


substrate_name: xanthine

substrate: xanthine


organism: Thermoplasma acidophilum

substrate_name: L-serine

substrate: L-serine


substrate_name: L-Serine; 5,6,7,8-Tetrahydrofolate

substrate: L-Serine; 5,6,7,8-Tetrahydrofolate


enzyme_display_name: glycogen phosphorylase

organism: Corynebacterium callunae

substrate_name: D-Glucose 1-phosphate

enzyme: glycogen phosphorylase

substrate: D-Glucose 1-phosphate


substrate_name: starch

substrate: starch


substrate_name: Starch; Phosphate

assay_conditions_summary: pH 7

substrate: Starch; Phosphate


organism: Escherichia coli

substrate_name: Maltoheptaose; Phosphate

substrate: Maltoheptaose; Phosphate


substrate_name: GMP

substrate: GMP


organism: Agrobacterium sp.

substrate_name: Unknown

substrate: Unknown


organism: Bacillus cereus

substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


organism: Bacillus subtilis


organism: Brucella anthropi

proof_lines: mutant E145G/N163H/N267S/P318R/M377V/M425T/P438L, pH 7.0, temperature not specified in the publication


proof_lines: mutant M425T, pH 7.0, temperature not specified in the publication


proof_lines: mutant N267S/P318R/M425T, pH 7.0, temperature not specified in the publication


proof_lines: mutant N267S, pH 7.0, temperature not specified in the publication


proof_lines: mutant P318R, pH 7.0, temperature not specified in the publication


organism: Oryctolagus cuniculus

substrate_name: Glycogen; D-Glucose 1-phosphate

substrate: Glycogen; D-Glucose 1-phosphate


organism: Solanum tuberosum


organism: Zea mays


organism: Homo sapiens


substrate_name: Hypoxanthine

substrate: Hypoxanthine


substrate_name: IMP

substrate: IMP


substrate_name: diphosphate

substrate: diphosphate


substrate_name: Guanine

substrate: Guanine


substrate_name: guanine

proof_lines:
at pH 7.4 and 37°C
at pH 7.4 and 25°C
above, pH 7.4, 25°C

substrate: guanine


proof_lines: 50 mM KCl, mutant E341Q, cosubstrate: 3-phosphoshikimate

proof_lines: 50 mM KCl, mutant E341Q, cosubstrate: phosphoenolpyruvate


substrate_name: shikimate 3-phosphate

substrate: shikimate 3-phosphate


substrate_name: Phosphoenolpyruvate

substrate: Phosphoenolpyruvate


proof_lines: 50 mM KCl, mutant D313A, cosubstrate: 3-phosphoshikimate

proof_lines: 50 mM KCl, mutant D313A, cosubstrate: phosphoenolpyruvate


proof_lines: 50 mM KCl, mutant K22A value above 0.3, cosubstrate: 3-phosphoshikimate

proof_lines: 50 mM KCl, mutant K22A value above 0.3, cosubstrate: phosphoenolpyruvate


proof_lines: 50 mM KCl, mutant K22R value above 0.3, cosubstrate: 3-phosphoshikimate

proof_lines: 50 mM KCl, mutant K22R value above 0.3, cosubstrate: phosphoenolpyruvate


proof_lines: wild-type, pH 7.0, temperature not specified in the publication


proof_lines:
+/-0.005 mM, mutant P101S
+/-0.006 mM, mutant P101S
mutant P101S


proof_lines: mutant G96A, in presence of 1 mM shikimate 3-phosphate


proof_lines: mutant G96A/A183T, in presence of 1 mM shikimate 3-phosphate


proof_lines: mutant A183T, in presence of 1 mM shikimate 3-phosphate


proof_lines: 50 mM KCl, mutant D313L, cosubstrate: 3-phosphoshikimate

proof_lines: 50 mM KCl, mutant D313L, cosubstrate: phosphoenolpyruvate


proof_lines: 50 mM KCl, mutant E341M, cosubstrate: 3-phosphoshikimate

proof_lines: 50 mM KCl, mutant E341M, cosubstrate: phosphoenolpyruvate


proof_lines: wild-type AroA, 100 mM KCl, cosubstrate: 3-phosphoshikimate

proof_lines: wild-type AroA, 100 mM KCl, cosubstrate: phosphoenolpyruvate


proof_lines: 50 mM KCl, mutant E341C, cosubstrate: 3-phosphoshikimate

proof_lines: 50 mM KCl, mutant E341C, cosubstrate: phosphoenolpyruvate


proof_lines: 50 mM KCl, mutant D313N, cosubstrate: 3-phosphoshikimate

proof_lines: 50 mM KCl, mutant D313N, cosubstrate: phosphoenolpyruvate


proof_lines: 50 mM KCl, mutant D313C, cosubstrate: 3-phosphoshikimate

proof_lines: 50 mM KCl, mutant D313C, cosubstrate: phosphoenolpyruvate


organism: Stutzerimonas stutzeri


substrate_name: Isopentenyl diphosphate; cis,trans,trans-Geranylgeranyl diphosphate


substrate_name: Isopentenyl diphosphate

smiles: C=C(C)CCOP(=O)(O)OP(=O)(O)O

substrate: Isopentenyl diphosphate


substrate_name: trans,trans-Farnesyl diphosphate

substrate: trans,trans-Farnesyl diphosphate


substrate_name: (2E,6E)-farnesyl diphosphate

substrate: (2E,6E)-farnesyl diphosphate


proof_lines: 50 mM KCl, mutant E341A, cosubstrate: phosphoenolpyruvate

proof_lines: 50 mM KCl, mutant E341A, cosubstrate: 3-phosphoshikimate


proof_lines: 50 mM KCl, mutant H385A, cosubstrate: 3-phosphoshikimate

proof_lines: 50 mM KCl, mutant H385A, cosubstrate: phosphoenolpyruvate


proof_lines: C-terminal His6-tagged AroA, 100 mM KCl, cosubstrate: 3-phosphoshikimate

proof_lines: C-terminal His6-tagged AroA, 100 mM KCl, cosubstrate: phosphoenolpyruvate

proof_lines: C-terminal His6-tagged AroA, 50 mM KCl, cosubstrate: 3-phosphoshikimate


organism: Halothermothrix orenii


organism: Janibacter sp.


organism: Oryza sativa


organism: Pseudomonas fluorescens

proof_lines: +/-0.00573 mM, full-length G2 EPSPS, crude lysate from overexpressing Escherichia coli ER2799, malachite green dye assay

proof_lines: +/-0.01101 mM, reconstituted G2-EPSPS (by intein-mediated protein complementation), crude lysate from transformed Escherichia coli ER2799, malachite green dye assay

proof_lines: +/-0.00578 mM, reconstituted G2-EPSPS (by intein-mediated protein complementation), crude lysate from transformed Escherichia coli ER2799, malachite green dye assay


organism: Rahnella aquatilis


organism: Streptococcus pneumoniae


substrate_name: Phosphate

smiles: O=P(O)(O)O

substrate: Phosphate


proof_lines: in the presence of 1 mM NH4Cl and 10 mM KCl


organism: Micrococcus luteus

substrate_name: (2Z,6E,10E)-geranylgeranyl diphosphate

substrate: (2Z,6E,10E)-geranylgeranyl diphosphate


enzyme_display_name: cysteine synthase

organism: Arabidopsis thaliana

substrate_name: O-Acetyl-L-serine

enzyme: cysteine synthase

substrate: O-Acetyl-L-serine


substrate_name: O-Acetyl-L-serine; Sulfide

substrate: O-Acetyl-L-serine; Sulfide


organism: Salmonella typhimurium

substrate_name: Hydrogen sulfide

smiles: S

substrate: Hydrogen sulfide


organism: Schizosaccharomyces pombe

substrate_name: O-Acetyl-L-serine; Hydrogen sulfide

substrate: O-Acetyl-L-serine; Hydrogen sulfide


substrate_name: Hydrogen sulfide; O-Acetyl-L-serine

substrate: Hydrogen sulfide; O-Acetyl-L-serine


organism: Bacillus anthracis

substrate_name: O-acetyl-L-serine

substrate: O-acetyl-L-serine


organism: Bacillus subtilis


organism: Caenorhabditis elegans

substrate_name: Cyanide

smiles: C#N

substrate: Cyanide


substrate_name: S-Sulfo-L-cysteine

substrate: S-Sulfo-L-cysteine


substrate_name: L-Cysteine

substrate: L-Cysteine


organism: Escherichia coli


assay_conditions_summary: pH 7.5; 298.15 K; cofactor CoA


substrate_name: Isopentenyl diphosphate; trans,trans-Farnesyl diphosphate


organism: Helicobacter pylori


organism: Lactobacillus plantarum

substrate_name: trans,trans-Farnesyl diphosphate; Isopentenyl diphosphate


substrate_name: Sulfide

substrate: Sulfide


organism: Leucaena leucocephala

proof_lines: pH 7.5, 22°C, recombinant enzymem, Michaelis-Menten model

proof_lines: pH 7.5, 22°C, recombinant enzyme, Lineweaver-Burke model


organism: Microcystis aeruginosa


organism: Mimosa pudica


substrate_name: hydrogen sulfide

substrate: hydrogen sulfide


substrate_name: Sulfide; O-Acetyl-L-serine

substrate: Sulfide; O-Acetyl-L-serine


substrate_name: O-acetyl-L-Ser

substrate: O-acetyl-L-Ser


substrate_name: L-Serine; Acetyl-CoA

assay_conditions_summary: cofactor CoA

substrate: L-Serine; Acetyl-CoA


substrate_name: O-Acetyl-L-serine; Thiosulfate

substrate: O-Acetyl-L-serine; Thiosulfate


substrate_name: (2Z,6E,10E)-geranylgeranyl diphosphate


substrate_name: (2E,6E)-farnesyl diphosphate

substrate: (2E,6E)-farnesyl diphosphate


substrate: (2Z,6E,10E)-geranylgeranyl diphosphate


proof_lines:
Raw SKiD Env: Temp=37°C, pH=7.5
Raw SKiD Env: Temp=37°C, pH=7.3
Raw SKiD Env: Temp=30°C, pH=7.5


organism: Aeropyrum pernix

substrate_name: O-Acetyl-L-serine

substrate: O-Acetyl-L-serine


substrate_name: O-Phospho-L-serine

substrate: O-Phospho-L-serine


substrate_name: Hydrogen sulfide

substrate: Hydrogen sulfide


substrate_name: Hydrogen sulfide; O-Acetyl-L-serine


substrate_name: O-Acetyl-L-serine; Sulfide

substrate: O-Acetyl-L-serine; Sulfide


organism: Streptomyces lavendulae

substrate_name: thiosulfate

substrate: thiosulfate


organism: Thermus thermophilus

substrate: Hydrogen sulfide; O-Acetyl-L-serine


enzyme_display_name: protein farnesyltransferase

organism: Rattus norvegicus

enzyme: protein farnesyltransferase


substrate_name: Farnesyl diphosphate; Protein cysteine

substrate: Farnesyl diphosphate; Protein cysteine


enzyme_display_name: aspartate transaminase

organism: Advenella mimigardefordensis

substrate_name: L-aspartate

enzyme: aspartate transaminase

substrate: L-aspartate


substrate_name: L-Aspartate

substrate: L-Aspartate


organism: Solanum tuberosum


organism: Spinacia oleracea


substrate_name: Sulfide

substrate: Sulfide


substrate_name: hydrogen sulfide

proof_lines: 30°C, pH 8.0, cosubstrate: hydrogen sulfide

substrate: hydrogen sulfide


substrate_name: L-Cysteine

substrate: L-Cysteine


organism: Arabidopsis thaliana

substrate_name: L-Aspartate; 2-Oxoglutarate

substrate: L-Aspartate; 2-Oxoglutarate


smiles: O=C(O)CCC(=O)C(=O)O


substrate_name: Prephenate

substrate: Prephenate


substrate_name: L-Aspartate; Prephenate

substrate: L-Aspartate; Prephenate


substrate_name: L-Glutamate

substrate: L-Glutamate


substrate_name: oxaloacetate

smiles: O=C(O)CC(=O)C(=O)O

substrate: oxaloacetate


proof_lines: pH 7.5, 25°C, recombinant wild-type enzyme


substrate_name: L-Tyrosine

substrate: L-Tyrosine


substrate_name: L-Phe

assay_conditions_summary: pH 8

proof_lines: pH 8, mutant enzyme A12T/P13T/N34D/T109S/G261A/S285G/A293D/N297S

proof_lines: pH 8, cosubstrate: L-Asp, mutant enzyme A12T/P13T/N34D/T109S/G261A/S285G/A293D/N297S

proof_lines: pH 8, cosubstrate: L-Phe, mutant enzyme A12T/P13T/N34D/T109S/G261A/S285G/A293D/N297S

substrate: L-Phe


substrate_name: L-glutamate

substrate: L-glutamate


proof_lines:
at pH 7.4 and 30°C
Raw SKiD Env: Temp=25°C, pH=7.5
Raw SKiD Env: Temp=30°C, pH=7.4


organism: Geobacillus thermopakistaniensis

proof_lines: using L-aspartate as a cosubstrate, at pH 8.0 and 60°C


organism: Homo sapiens


organism: Mycobacterium tuberculosis


substrate_name: kynurenine

substrate: kynurenine


organism: Panicum miliaceum


organism: Petunia hybrida


substrate_name: L-Arogenate

substrate: L-Arogenate


organism: Bacillus sp.


organism: Bacillus subtilis


organism: Escherichia coli


enzyme_display_name: branched-chain-amino-acid transaminase

organism: Thermococcus sp.

enzyme: branched-chain-amino-acid transaminase


substrate_name: L-norleucine

substrate: L-norleucine


smiles: CC(C)CC(=O)C(=O)O


substrate_name: L-Isoleucine

substrate: L-Isoleucine


proof_lines: half transamination reaction, pH 8.0, 90°C


substrate_name: L-norvaline

substrate: L-norvaline


substrate_name: phenylpyruvate

substrate: phenylpyruvate


substrate_name: L-Valine

substrate: L-Valine


smiles: CC(C)C(=O)C(=O)O


organism: Burkholderia mallei

assay_conditions_summary: pH 7.9; 298.15 K; cofactor NADH


substrate_name: crotonyl-[acyl-carrier protein]

proof_lines: substrate 2-dodecenoyl-CoA, pH 7.9, 25°C

substrate: crotonyl-[acyl-carrier protein]


organism: Burkholderia pseudomallei

assay_conditions_summary: pH 6.8; 298.15 K; cofactor NADH

proof_lines:
pH 6.8, 25°C
Raw SKiD Env: Temp=25°C, pH=6.8


substrate_name: L-Leucine

substrate: L-Leucine


organism: Pinus pinaster

substrate_name: L-Aspartate; 2-Oxoglutarate

substrate: L-Aspartate; 2-Oxoglutarate


organism: Rattus norvegicus


organism: Rattus rattus


organism: Rhizobium meliloti


organism: Saccharolobus solfataricus

substrate_name: L-aspartate

substrate: L-aspartate


organism: Sinorhizobium meliloti

proof_lines:
pH 8.0, 30°C
Raw SKiD Env: Temp=30°C, pH=8


smiles: CC(C)(C)CC(=O)C(=O)O


proof_lines: cosubstrate 4,4-dimethyl-2-oxopentanoate, pH 8.0, 37°C


organism: Gluconobacter oxydans


organism: Haliangium ochraceum


proof_lines:
pH 10.0, 40°C
Raw SKiD Env: Temp=40°C, pH=10


substrate_name: L-leucine

substrate: L-leucine


organism: Helicobacter pylori

substrate_name: L-isoleucine

substrate: L-isoleucine


organism: Homo sapiens

substrate_name: L-Leucine; 2-Oxoglutarate

substrate: L-Leucine; 2-Oxoglutarate


substrate_name: L-Glutamate

substrate: L-Glutamate


substrate_name: L-Isoleucine; alpha-Ketovaline

substrate: L-Isoleucine; alpha-Ketovaline


proof_lines:
cytosolic isoform, oxidized
cytosolic isoform, reduced
mitochondrial isoform, reduced


organism: Sus scrofa


organism: Thermus thermophilus


organism: Triticum aestivum

proof_lines:
allozyme AAT-2a, pH 7.5, 25°C
allozyme AAT-2b, pH 7.5, 25°C
allozyme AAT-2c, pH 7.5, 25°C


proof_lines:
allozyme AAT-3b, pH 7.5, 25°C
allozyme AAT-3c, pH 7.5, 25°C
allozyme AAT-3a, pH 7.5, 25°C


substrate_name: L-Aspartate

substrate: L-Aspartate


organism: Trypanosoma brucei


organism: Trypanosoma cruzi


substrate_name: L-tyrosine

proof_lines:
pH 7.5, 37°C
Raw SKiD Env: Temp=37°C, pH=7.5

substrate: L-tyrosine


substrate_name: L-valine

substrate: L-valine


organism: Methanococcus aeolicus

substrate_name: L-Isoleucine; 2-Ketoglutaric acid

substrate: L-Isoleucine; 2-Ketoglutaric acid


organism: Mycobacterium tuberculosis

substrate_name: L-glutamate

proof_lines:
pH 7.4, 37°C
Raw SKiD Env: Temp=37°C, pH=7.4

substrate: L-glutamate


organism: Pseudomonas aeruginosa

proof_lines:
pH 8.0, 37°C
Raw SKiD Env: Temp=37°C, pH=8


organism: Rattus norvegicus


organism: Synechocystis sp.


organism: Thermobaculum terrenum

proof_lines: pH 9.0, 50°C, recombinant enzyme, with 2-oxogluarate


organism: Thermococcus sp.

smiles: CCCCC(=O)C(=O)[O-]


organism: Arabidopsis thaliana

substrate_name: L-Glutamate; 3-Methyl-2-oxobutanoate

substrate: L-Glutamate; 3-Methyl-2-oxobutanoate


organism: Bacillus anthracis


organism: Bacillus cereus


organism: Bacillus subtilis


organism: Brevibacillus brevis


organism: Escherichia coli


smiles: CCCCCC(N)C(=O)O


substrate_name: L-Phenylalanine

substrate: L-Phenylalanine


smiles: CCCCCCC(N)C(=O)O


organism: Thermoproteus uzoniensis


substrate_name: pyruvate

smiles: CC(=O)C(=O)O

substrate: pyruvate


substrate_name: L-alanine

substrate: L-alanine


organism: Vulcanisaeta moutnovskia

substrate_name: L-Ornithine

substrate: L-Ornithine


proof_lines:
pH 8.0, 65°C
Raw SKiD Env: Temp=65°C, pH=8


enzyme_display_name: acetate kinase

organism: Methanosarcina thermophila

substrate_name: Acetate

smiles: CC(=O)O

enzyme: acetate kinase

substrate: Acetate


substrate_name: ATP

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

substrate: ATP


substrate_name: ADP

assay_conditions_summary: pH 7.4; cofactor ATP

proof_lines: mutant R91A, pH 7.0, presence of 200 mM guanidine/HCl

substrate: ADP


assay_conditions_summary: pH 7; cofactor ATP

proof_lines:
mutant R91K, pH 7.0
mutant R91K, pH 7.4


substrate_name: ATP; D-Fructose 6-phosphate

assay_conditions_summary: cofactor ATP

substrate: ATP; D-Fructose 6-phosphate


substrate_name: beta-D-Fructose 6-phosphate; ATP

substrate: beta-D-Fructose 6-phosphate; ATP


substrate_name: D-Fructose 6-phosphate

substrate: D-Fructose 6-phosphate


assay_conditions_summary: pH 6.5; cofactor ATP


organism: Spinacia oleracea

substrate_name: beta-D-fructose 6-phosphate

assay_conditions_summary: pH 7.8; 298.15 K; cofactor ATP

proof_lines:
pH 7.8, 25°C, effect of phosphate on Km
pH 7.8, 25°C

substrate: beta-D-fructose 6-phosphate


substrate_name: L-Valine

substrate: L-Valine


substrate_name: L-norvaline

substrate: L-norvaline


substrate_name: L-valine

substrate: L-valine


assay_conditions_summary: pH 6; 298.15 K; cofactor ATP


organism: Bos taurus


organism: Gallus gallus


organism: Homo sapiens

substrate_name: D-Fructose 6-phosphate; ATP

substrate: D-Fructose 6-phosphate; ATP


mutation_type: deletion

proof_lines: pH 7.5, 30°C, recombinant His-tagged HBP1 deletion mutant


mutation_signature: is-tagged HBP1 deletion mutant

proof_lines: pH 7.5, 30°C, recombinant Hnon-phosphorylated, is-tagged HBP1 deletion mutant


proof_lines: pH 7.5, 30°C, recombinant His-tagged HBP1 mutant S302R

proof_lines: pH 7.5, 30°C, recombinant non-phosphorylated, His-tagged HBP1 mutant S302R


proof_lines: pH 7.5, 30°C, recombinant HBP1 mutant S460D

proof_lines: pH 7.5, 30°C, recombinant non-phosphorylated, HBP1 mutant S460D


proof_lines: pH 7.5, 30°C, recombinant HBP1 mutant H253A

proof_lines: pH 7.5, 30°C, recombinant non-phosphorylated, HBP1 mutant H253A


substrate_name: ADP; Acetyl phosphate

substrate: ADP; Acetyl phosphate


organism: Fructilactobacillus sanfranciscensis

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP


organism: Lactococcus lactis

substrate_name: acetate

proof_lines: pH 7.5, 30°C, recombinant His-tagged isozyme AckA1

substrate: acetate


proof_lines: pH 7.5, 30°C, recombinant His-tagged isozyme AckA2


organism: Methanosarcina mazei

proof_lines: pH 7.0, temperature not specified in the publication


organism: Trypanosoma brucei

assay_conditions_summary: pH 7.1; 303.15 K; cofactor ATP

proof_lines: pH 7.1, 30°C, protein kinase A treated enzyme


organism: Enterococcus faecalis

substrate_name: ATP; Acetate

substrate: ATP; Acetate


assay_conditions_summary: pH 7.5; 283.15 K; cofactor ATP


substrate_name: acetyl phosphate

assay_conditions_summary: pH 7.5; 288.15 K; cofactor ATP

proof_lines:
pH 7.5, 15°C, recombinant enzyme
pH 7.5, 10°C, recombinant enzyme
pH 7.5, 25°C, recombinant enzyme

substrate: acetyl phosphate


assay_conditions_summary: pH 6.5; 303.15 K; cofactor ATP


proof_lines: pH 6.5, 30°C, recombinant mutant N213T/G332D/E336L/T385N


proof_lines: pH 6.5, 30°C, recombinant wild-type enzyme


proof_lines: pH 7.5, 30°C, recombinant HBP1 mutant S460D/T470D


proof_lines: pH 7.5, 30°C, recombinant HBP1 mutant S477D

proof_lines: pH 7.5, 30°C, recombinant non-phosphorylated, HBP1 mutant S477D


proof_lines: pH 7.5, 30°C, recombinant HBP1 mutant H253A/S460D

proof_lines: pH 7.5, 30°C, recombinant non-phosphorylated, HBP1 mutant H253A/S460D


proof_lines: pH 7.5, 30°C, recombinant non-phosphorylated, HBP1 mutants T470D and S460D/T470D


proof_lines: pH 7.5, 30°C, recombinant His-tagged wild-type HBP1

proof_lines: pH 7.5, 30°C, recombinant His-tagged wild-type HBP2

proof_lines: pH 7.5, 30°C, recombinant wild-type HBP1


proof_lines: pH 7.5, 30°C, recombinant HBP1 mutant T470

proof_lines: pH 7.5, 30°C, recombinant non-phosphorylated, His-tagged chimeric mutant enzyme

proof_lines: pH 7.5, 30°C, recombinant phosphorylated, His-tagged chimeric mutant enzyme


assay_conditions_summary: pH 8.5; 298.15 K; cofactor ATP


proof_lines: inhibition of recombinant PFKFB3 protein, no inhibition of purified PFK-1 activity


substrate_name: ATP; beta-D-Fructose 6-phosphate

substrate: ATP; beta-D-Fructose 6-phosphate


organism: Rattus norvegicus


enzyme_display_name: creatine kinase

substrate_name: Phosphocreatine

smiles: CN(CC(=O)O)C(=N)NP(=O)(O)O

enzyme: creatine kinase

substrate: Phosphocreatine


substrate_name: Creatine

smiles: CN(CC(=O)O)C(=N)N

substrate: Creatine


assay_conditions_summary: pH 7; 303.15 K; cofactor ADP


substrate_name: ATP

assay_conditions_summary: pH 9; 303.15 K; cofactor ATP

substrate: ATP


assay_conditions_summary: pH 8; 298.15 K; cofactor ATP

proof_lines: recombinant mutant enzyme H66P/D326A, at 25Â°C, pH not specified in the publication

proof_lines: mutant enzyme H66P/D326A, at pH 8.0 and 25°C


proof_lines: recombinant wild type enzyme, at 25Â°C, pH not specified in the publication


proof_lines: recombinant mutant enzyme H66P, at 25Â°C, pH not specified in the publication


proof_lines: recombinant mutant enzyme H66R, at 25Â°C, pH not specified in the publication


assay_conditions_summary: pH 7; 310.15 K; cofactor ATP


substrate_name: ITP

substrate: ITP


substrate_name: TTP

substrate: TTP


substrate_name: UTP

substrate: UTP


substrate_name: GTP

substrate: GTP


substrate_name: CTP

substrate: CTP


proof_lines:
37°C, pH 7.0-7.5, wild-type enzyme
wild-type, pH 7.4
wild-type


proof_lines:
mutant R241L, pH 7.0
mutant R241L, pH 7.4


substrate_name: ADP

proof_lines: mutant R241A, pH 7.0, presence of 200 mM guanidine/HCl

substrate: ADP


proof_lines:
mutant R91L, pH 7.4
mutant R91L, pH 7.0


proof_lines:
mutant R241K, pH 7.0
mutant R241K, pH 7.4


proof_lines: recombinant mutant enzyme D326E, at 25Â°C, pH not specified in the publication


proof_lines: recombinant mutant enzyme D326A, at 25Â°C, pH not specified in the publication


substrate_name: ADP; Phosphocreatine

substrate: ADP; Phosphocreatine


substrate_name: Phosphocreatine; dCDP

substrate: Phosphocreatine; dCDP


proof_lines:
Raw SKiD Env: Temp=25°C, pH=8
Raw SKiD Env: Temp=25Â°C, pH=-----


organism: Oryctolagus cuniculus


substrate_name: ATP; Creatine

substrate: ATP; Creatine


assay_conditions_summary: 298.15 K; cofactor ATP


assay_conditions_summary: pH 9; 298.15 K; cofactor ATP

proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=25°C, pH=-----
Raw SKiD Env: Temp=30°C, pH=9


enzyme_display_name: adenylate kinase

organism: Arabidopsis thaliana

substrate_name: ATP; AMP

enzyme: adenylate kinase

substrate: ATP; AMP


organism: Bacillus subtilis

assay_conditions_summary: pH 7.2; 328.15 K; cofactor ATP

proof_lines:
Raw SKiD Env: Temp=55°C, pH=7.2
Raw SKiD Env: Temp=45°C, pH=7.2
Raw SKiD Env: Temp=35°C, pH=7.2


substrate_name: AMP

proof_lines:
wild-type, pH 7.2, 55°C
wild-type, pH 7.2, 45°C
wild-type, pH 7.2, 27°C

substrate: AMP


assay_conditions_summary: pH 5.5; 310.15 K; cofactor ATP

proof_lines: 37°C, pH is not specified in the publication

proof_lines: pH 5.5, temperature not specified in the publication

proof_lines: pH and temperature not specified in the publication


assay_conditions_summary: pH 7; 355.15 K; cofactor ATP


substrate_name: acetate

assay_conditions_summary: pH 5.5; 355.15 K; cofactor ATP

substrate: acetate


substrate_name: acetyl phosphate

smiles: CC(=O)OP(=O)(O)O

substrate: acetyl phosphate


assay_conditions_summary: pH 7; cofactor ATP

proof_lines: mutant enzyme G331Q/I332M, at pH 7.0 and 37Â°C

proof_lines: mutant enzyme G331Q/I332M, at pH 5.5 and 45Â°C


organism: Methylotuvimicrobium alcaliphilum

assay_conditions_summary: pH 8; 303.15 K; cofactor ATP


organism: Porphyromonas gingivalis

assay_conditions_summary: pH 8; cofactor ATP


organism: Salmonella enterica subsp. enterica serovar Typhimurium

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

proof_lines:
pH 7.5, 37°C
co-substrate: acetate, pH 7.5, 37°C
co-substrate: propionate, pH 7.5, 37°C


organism: Shewanella sp.

assay_conditions_summary: pH 7.5; 283.15 K; cofactor ATP

proof_lines:
pH 7.5, 10°C, recombinant enzyme
pH 7.5, 15°C, recombinant enzyme
pH 7.5, 25°C, recombinant enzyme


organism: Cryptosporidium muris

substrate_name: glycocyamine

smiles: N=C(N)NCC(=O)O

substrate: glycocyamine


assay_conditions_summary: cofactor ATP


proof_lines:
ATP in form of MgATP2-
Raw SKiD Env: Temp=30°C, pH=9
Raw SKiD Env: Temp=-----, pH=-----


substrate_name: N-ethylglycocyamine

proof_lines: pH 9.0, 30°C, recombinant wild-type enzyme, with substrate N-ethylglycocyamine

proof_lines: pH 9.0, 30°C, recombinant wild-type enzyme, with substrate creatine

substrate: N-ethylglycocyamine


substrate_name: Phosphocreatine

substrate: Phosphocreatine


proof_lines:
mutant Q199R, pH 7.2, 60°C
mutant Q199R, pH 7.2, 45°C
mutant Q199R, pH 7.2, 55°C


organism: Bordetella pertussis


organism: Bos taurus


assay_conditions_summary: cofactor ADP


organism: Clonorchis sinensis

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ADP

proof_lines: in 100 mM Tris–HCl (pH 7.5), 20 mM glucose, 5 mM MgCl2, 10 mM KCl, 2 mM dithiothreitol, at 37°C


organism: Cyprinus carpio


organism: Escherichia coli

proof_lines: closed conformation, pH and temperature not specified in the publication

proof_lines: open conformation, pH and temperature not specified in the publication


organism: Gallus gallus


organism: Geobacillus stearothermophilus


organism: Homo sapiens

proof_lines: nucleotide-binding domain 1 of wild type

proof_lines: nucleotide-binding domain 2 of wild type


substrate_name: ATP

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP

proof_lines:
mutant D140del, 37°C, pH 8.0
mutant D140del, 37°C

substrate: ATP


organism: Plasmodium falciparum

assay_conditions_summary: pH 6; cofactor ATP


assay_conditions_summary: pH 7.6; 298.15 K; buffer TEA-HCl; cofactor ATP

proof_lines: recombinant adenylate kinase 2, in 110 mM TEA-HCl, pH 7.6, at 25°C


organism: Saccharomyces cerevisiae


organism: Streptococcus pneumoniae

substrate_name: Unknown

substrate: Unknown


organism: Strongylocentrotus purpuratus

assay_conditions_summary: pH 7.6; 289.15 K; cofactor ADP


organism: Sulfolobus acidocaldarius

assay_conditions_summary: 343.15 K; cofactor ATP

proof_lines: 70°C, pH is not specified in the publication

proof_lines: 90°C, pH is not specified in the publication


organism: Thermotoga neapolitana

assay_conditions_summary: pH 7.4; 303.15 K; cofactor ATP


substrate_name: ATP; Nicotinamide nucleotide

assay_conditions_summary: cofactor NAD+

substrate: ATP; Nicotinamide nucleotide


substrate_name: nicotinamide ribonucleotide

substrate: nicotinamide ribonucleotide


proof_lines:
mutant G64R, 37°C, pH 8.0
mutant G64R, 37°C


proof_lines:
mutant Y164C, 37°C, pH 8.0
mutant Y164C, 37°C


proof_lines:
mutant G40R, 37°C, pH 8.0
mutant G40R, 37°C


assay_conditions_summary: 310.15 K; cofactor ATP


proof_lines:
wild-type, 37°C, pH 8.0
wild-type, 37°C


proof_lines: Km above 2.0 mM, adenylate kinase 5 domain AK5p1, with ATP as phosphate donor


proof_lines: recombinant adenylate kinase 4, using GTP as cosubstrate

proof_lines: recombinant adenylate kinase 4, using ATP as cosubstrate


assay_conditions_summary: pH 8; 310.15 K; cofactor ADP

proof_lines:
Raw SKiD Env: Temp=37°C, pH=-----
Raw SKiD Env: Temp=37°C, pH=8


assay_conditions_summary: pH 7.5; cofactor ATP

proof_lines: mutant enzyme CINAP H79G, in 100 mM Tris-HCl, pH 7.5, 60 mM KCl, temperature not specified in the publication


substrate_name: Nicotinamide nucleotide

substrate: Nicotinamide nucleotide


substrate_name: Tiazofurin monophosphate

substrate: Tiazofurin monophosphate


substrate_name: ITP

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

substrate: ITP


substrate_name: nicotinic acid mononucleotide

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NAD+

proof_lines: product inhibition of NMNAT3, substrate NMN

substrate: nicotinic acid mononucleotide


substrate_name: Nicotinate D-ribonucleotide

substrate: Nicotinate D-ribonucleotide


assay_conditions_summary: pH 7.5; 315.15 K; cofactor ATP


organism: Leishmania braziliensis


assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP


organism: Methanothermobacter thermautotrophicus

assay_conditions_summary: pH 7.5; 338.15 K; cofactor ATP


assay_conditions_summary: pH 7.5; 338.15 K; cofactor NAD+


organism: Giardia duodenalis

assay_conditions_summary: pH 7.5; 299.15 K; cofactor ATP

proof_lines: pH 7.5, 26°C, recombinant isozyme GlNMNAT-A


organism: Haemophilus influenzae

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP


substrate_name: Nicotinamide nucleotide; ATP

substrate: Nicotinamide nucleotide; ATP


substrate_name: GTP

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NAD+

substrate: GTP


substrate_name: ATP; Tiazofurin monophosphate

substrate: ATP; Tiazofurin monophosphate


proof_lines: wild type enzyme CINAP, in 100 mM Tris-HCl, pH 7.5, 60 mM KCl, temperature not specified in the publication


substrate_name: AMP

assay_conditions_summary: cofactor ATP

substrate: AMP


organism: Jeotgalibacillus marinus

assay_conditions_summary: pH 7.2; 303.15 K; buffer the same phosphate; cofactor ATP

proof_lines: at 30°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 65 mM KCl

proof_lines: at 22°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 65 mM KCl

proof_lines: at 40°C, in 25 mM phosphate buffer pH 7.2, 5 mM MgCl2, 65 mM KCl


organism: Leishmania donovani

assay_conditions_summary: pH 8; cofactor ATP


organism: Megalodesulfovibrio gigas

assay_conditions_summary: pH 7.6; buffer the same Tris-HCl; cofactor ATP

proof_lines: Co2+-containing recombinant adenylate kinase, in 50 mM Tris-HCl, pH 7.6, 100 mM KCl, 0.25 mM MgCl2

proof_lines: Zn2+-containing recombinant adenylate kinase, in 50 mM Tris-HCl, pH 7.6, 100 mM KCl, 0.25 mM MgCl2

proof_lines: Fe2+-containing recombinant adenylate kinase, in 50 mM Tris-HCl, pH 7.6, 100 mM KCl, 0.25 mM MgCl2


organism: Methanococcus maripaludis

assay_conditions_summary: pH 7.2; 313.15 K; buffer the same phosphate; cofactor ATP

proof_lines: at 40°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2

proof_lines: at 30°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2

proof_lines: at 50°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2


organism: Mus musculus


organism: Oryctolagus cuniculus

substrate_name: ATP; AMP

substrate: ATP; AMP


organism: Penicillium chrysogenum


assay_conditions_summary: cofactor NAD+


organism: Plasmodium falciparum


organism: Salmonella enterica


organism: Salmonella typhimurium


enzyme_display_name: acetylcholinesterase

organism: Bungarus fasciatus

substrate_name: H2O; Acetylcholine

enzyme: acetylcholinesterase

substrate: H2O; Acetylcholine


organism: Dictyocaulus viviparus

substrate_name: Acetylthiocholine; H2O

substrate: Acetylthiocholine; H2O


enzyme_display_name: alkaline phosphatase

organism: Escherichia coli

substrate_name: 4-nitrophenyl phosphate

proof_lines:
pH 8.0, 25°C, mutant R166S
mutant enzyme R166S, at pH 8.0 and 25°C

enzyme: alkaline phosphatase

substrate: 4-nitrophenyl phosphate


proof_lines: mutant enzyme R166S/D153A/E322Y, at pH 8.0 and 25°C


proof_lines: mutant enzyme R166S/D153A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/D153A/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/R166S/D153A/E322Y/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme R166S/E322Y, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/R166S/E322Y, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/E322Y/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D153A/E322Y/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme R166S/D153A/E322Y/K328A, at pH 8.0 and 25°C


organism: Electrophorus electricus

substrate_name: H2O; Acetylthiocholine

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: H2O; Acetylthiocholine


organism: Homo sapiens

proof_lines: recombinant enzyme, pH and temperature not specified in the publication


substrate_name: Acetylthiocholine

smiles: CC(=O)SCC[N+](C)(C)C

substrate: Acetylthiocholine


substrate_name: acetylthiocholine

substrate: acetylthiocholine


organism: Stomoxys calcitrans

assay_conditions_summary: pH 7.5; 298.15 K; buffer potassium phosphate

proof_lines: in potassium phosphate buffer (100 mM, pH 7.5), at 25°C


proof_lines: mutant enzyme E322Y/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/E322Y, at pH 8.0 and 25°C


proof_lines: mutant enzyme D153A/E322Y, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/D153A/E322Y, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/R166S, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/R166S/D153A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D153A/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/R166S/D153A/E322Y, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/D153A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/R166S/D153A/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme R166S/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme R166S/D153A/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/D153A/E322Y/K328A, at pH 8.0 and 25°C


organism: Antarctic bacterium TAB5


substrate_name: 4-Nitrophenyl phosphate

substrate: 4-Nitrophenyl phosphate


proof_lines: pH 8.0, 25°C, recombinant wild-type enzyme


substrate_name: H2O; 4-Nitrophenyl phosphate

assay_conditions_summary: buffer contexts

substrate: H2O; 4-Nitrophenyl phosphate


proof_lines: mutant enzyme D101A/R166S/E322Y/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme R166S/E322Y/K328A, at pH 8.0 and 25°C


proof_lines: mutant enzyme D101A/R166S/K328A, at pH 8.0 and 25°C


substrate_name: methyl phosphate

substrate: methyl phosphate


organism: Gallus gallus


organism: Geobacillus thermodenitrificans


organism: Halobacterium salinarum

proof_lines:
pH 10.5, 20°C, 4 M NaCl
Raw SKiD Env: Temp=20°C, pH=10.5


organism: Helicoverpa armigera


substrate_name: H2O; Phenolic phosphate

substrate: H2O; Phenolic phosphate


organism: Bacillus licheniformis


organism: Bos taurus

substrate_name: 3-(4-methoxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]-decan]-4-yl)phenyl phosphate

proof_lines: direct physical adsorption, immobilized ALP


substrate_name: Phosphoenolpyruvate; H2O

substrate: Phosphoenolpyruvate; H2O


organism: Burkholderia cenocepacia

substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


organism: Cobetia marina


proof_lines:
25°C, pH 18.0, Tris-HCl buffer
25°C, pH 10.3, diethanolamine buffer
25°C, pH 18.0, diethanolamine buffer


substrate_name: 4-Nitrophenyl phosphate; H2O

proof_lines:
single-chain Fv antibody fusion protein
Raw SKiD Env: Temp=25°C, pH=8
Raw SKiD Env: Temp=-----, pH=-----

substrate: 4-Nitrophenyl phosphate; H2O


organism: Mus musculus

substrate_name: Unknown

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: Unknown


organism: Rattus norvegicus


organism: Saccharococcus caldoxylosilyticus

proof_lines: pH 9.5, 50°C, Vmax: 0.049 micromol/min/mg


organism: Shewanella sp. T3-3


organism: Sphingobium sp. TCM1


organism: Thermus sp.


organism: Thermus sp. NTU-237


organism: Escherichia coli

substrate_name: GMP

substrate: GMP


substrate_name: AMP

substrate: AMP


substrate_name: dGMP

substrate: dGMP


substrate_name: dTMP

substrate: dTMP


substrate_name: dUMP

substrate: dUMP


substrate_name: UMP

substrate: UMP


substrate_name: dAMP

substrate: dAMP


substrate_name: dCMP

substrate: dCMP


substrate_name: dIMP

substrate: dIMP


substrate_name: 4-Nitrophenyl phosphate

substrate: 4-Nitrophenyl phosphate


substrate_name: H2O; 4-Nitrophenyl phosphate

substrate: H2O; 4-Nitrophenyl phosphate


organism: Thermus thermophilus

substrate_name: 4-nitrophenyl phosphate

substrate: 4-nitrophenyl phosphate


organism: Vibrio sp. G15-21

proof_lines: mutant Y423F, presence of 0.5 M NaCl, pH 8, 10°C


proof_lines: mutant A221D, presence of 0.5 M NaCl, pH 8, 10°C


proof_lines: mutant K422E, presence of 0.5 M NaCl, pH 8, 10°C


proof_lines: mutant K422L/Y423F, presence of 0.5 M NaCl, pH 8, 10°C


proof_lines: mutant K422L, presence of 0.5 M NaCl, pH 8, 10°C


proof_lines: mutant Y222P, presence of 0.5 M NaCl, pH 8, 10°C


proof_lines: wild-type, presence of 0.5 M NaCl, pH 8, 10°C


organism: Bacillus subtilis


substrate_name: D-Ribose 5-phosphate

substrate: D-Ribose 5-phosphate


organism: Homo sapiens

substrate_name: H2O; AMP

substrate: H2O; AMP


substrate_name: ATP; D-2'-Deoxycarbocyclic guanosine

substrate: ATP; D-2'-Deoxycarbocyclic guanosine


substrate_name: H2O; IMP

substrate: H2O; IMP


substrate_name: H2O; dUMP

substrate: H2O; dUMP


organism: Boophilus microplus


organism: Drosophila melanogaster


substrate_name: H2O; GMP

substrate: H2O; GMP


substrate_name: GMP

substrate: GMP


substrate_name: dGMP

substrate: dGMP


substrate_name: AMP; H2O

substrate: AMP; H2O


organism: Helicobacter pylori


substrate_name: Inosine

proof_lines: presence of 2 mM ATP, Tris-buffer, pH 7.4

proof_lines: presence of 0.4 mM 2,3-bisphosphoglycerate, Tris-buffer, pH 7.4

proof_lines: presence of 2 mM ATP, phosphate-buffered saline, pH 7.4

substrate: Inosine


substrate_name: CMP

substrate: CMP


substrate_name: H2O; CMP

substrate: H2O; CMP


substrate_name: AMP

substrate: AMP


organism: Mycoplasma pulmonis

substrate_name: H2O; 5'-AMP

substrate: H2O; 5'-AMP


enzyme_display_name: alpha-amylase

organism: Alteromonas haloplanktis

enzyme: alpha-amylase


organism: Anabaena sp.

substrate_name: oyster glycogen

assay_conditions_summary: pH 7; 303.15 K; buffer 10 mM MOPS

proof_lines: at 30°C in 10 mM MOPS buffer (pH 7.0) with 5 mM CaCl2

substrate: oyster glycogen


organism: Bacillus amyloliquefaciens

substrate_name: starch

substrate: starch


substrate_name: soluble starch

proof_lines:
wild-type, pH 6, 60°C
wild type enzyme

substrate: soluble starch


organism: Bacillus licheniformis

proof_lines: determined with Lineweaver-Burk plot, competitive inhibition, pH 7.4, 37°C, docking simulations show that Tris binds deep inside the active site of the enzyme


organism: Hordeum vulgare

proof_lines:
below, pH 5.5, 37°C, mutant Y105A
above, pH 5.5, 37°C, mutant Y105A


substrate_name: amylose DP17

substrate: amylose DP17


substrate_name: amylose DP440

substrate: amylose DP440


proof_lines: Y380M mutant, pH 5.5, 30°C, kcat/Km: 39 1/s/mg/ml

proof_lines: Y380M mutant, pH 5.5, 30°C, kcat/Km: 425 1/s/mg/ml


proof_lines: Y380A mutant, pH 5.5, 30°C, kcat/Km: 28 1/s/mg/ml

proof_lines: Y380A mutant, pH 5.5, 30°C, kcat/Km: 264 1/s/mg/ml


proof_lines: Y105A mutant, kcat below 10 1/s, pH 5.5, 30°C

proof_lines: Y105A mutant, pH 5.5, 30°C, kcat/Km: 132 1/s/mg/ml, kcat/Km decreases 10-25fold for all Y105 mutants

proof_lines: Y105A mutant, Km above 10 mM, kcat below 10 1/s, pH 5.5, 30°C


proof_lines: Y105A/Y380M mutant, kcat below 10 1/s, pH 5.5, 30°C

proof_lines: Y105A/Y380M mutant, pH 5.5, 30°C, kcat/Km: 56 1/s/mg/ml, kcat/Km decreases 10-25fold for all Y105 mutants

proof_lines: Y105A/Y380M mutant, Km above 10 mM, kcat below 10 1/s, pH 5.5, 30°C


proof_lines: wild-type enzyme, pH 5.5, 30°C, kcat/Km: 48 1/s/mg/ml

proof_lines: wild-type enzyme, pH 5.5, 30°C, kcat/Km: 1300 1/s/mg/ml

proof_lines: wild-type enzyme, pH 5.5, 30°C, kcat/Km: 38 1/s/mg/ml


proof_lines: Y105A/Y380A mutant, kcat below 10 1/s, pH 5.5, 30°C

proof_lines: Y105A/Y380A mutant, pH 5.5, 30°C, kcat/Km: 51 1/s/mg/ml, kcat/Km decreases 10-25fold for all Y105 mutants

proof_lines: Y105A/Y380A mutant, Km above 10 mM, pH 5.5, 30°C, kcat/Km: 1300 1/s/mg/ml


enzyme_display_name: alpha-glucosidase

organism: Apis mellifera

substrate_name: Phenyl alpha-D-glucoside

enzyme: alpha-glucosidase

substrate: Phenyl alpha-D-glucoside


substrate_name: maltopentaose

substrate: maltopentaose


substrate_name: Nigerose

substrate: Nigerose


substrate_name: turanose

substrate: turanose


substrate_name: maltotetraose

substrate: maltotetraose


organism: Aspergillus nidulans

substrate_name: H2O; Maltose

substrate: H2O; Maltose


substrate_name: alpha,alpha-Trehalose

substrate: alpha,alpha-Trehalose


substrate_name: Maltose

substrate: Maltose


substrate_name: Isomaltose

substrate: Isomaltose


substrate_name: Maltotriose

substrate: Maltotriose


organism: Apis cerana indica


substrate_name: isomaltose

substrate: isomaltose


substrate_name: 4-nitrophenyl alpha-D-glucoside

substrate: 4-nitrophenyl alpha-D-glucoside


substrate_name: sucrose

substrate: sucrose


substrate_name: p-Nitrophenyl-alpha-D-glucoside

substrate: p-Nitrophenyl-alpha-D-glucoside


substrate_name: Sucrose

substrate: Sucrose


substrate_name: Kojibiose

substrate: Kojibiose


substrate_name: Maltohexaose

substrate: Maltohexaose


organism: Sus scrofa

proof_lines: pH not specified in the publication, 25°C

proof_lines: presence of 4.8 mM neohesperidin dihydrochalcone, pH 7.2, 42°C


organism: Tepidimonas fonticaldi

substrate_name: soluble potato starch

substrate: soluble potato starch


organism: Thermobifida fusca

proof_lines: pH not specified in the publication, 55°C


organism: Thermotoga neapolitana

substrate_name: maltopentaose

substrate: maltopentaose


substrate_name: 4-nitrophenyl alpha-D-maltohexaoside

substrate: 4-nitrophenyl alpha-D-maltohexaoside


assay_conditions_summary: pH 6.5; 348.15 K; buffer 50 mM sodium phosphate

proof_lines: in 50 mM sodium phosphate buffer (pH 6.5) at 75°C


substrate_name: maltotriose

substrate: maltotriose


substrate_name: maltoheptaose

substrate: maltoheptaose


organism: Tribolium castaneum

substrate_name: amylopectin

substrate: amylopectin


organism: uncultured organism


organism: Vigna angularis

substrate_name: Amylose; H2O

substrate: Amylose; H2O


organism: Helicoverpa armigera

substrate_name: starch

substrate: starch


organism: Homo sapiens

substrate_name: Maltotriose; H2O

proof_lines:
in presence of 1 mM NO2-
in presence of 0.2 mM ClO3-
in presence of 0.01 mM I-

substrate: Maltotriose; H2O


substrate_name: Maltotriose

substrate: Maltotriose


substrate_name: amylose DP17

substrate: amylose DP17


substrate_name: Nigerose

substrate: Nigerose


organism: Bacillus sp.

substrate_name: H2O; Trehalose

substrate: H2O; Trehalose


organism: Caldanaerobacter subterraneus subsp. tengcongensis

substrate_name: 4-nitrophenyl alpha-D-glucopyranoside

substrate: 4-nitrophenyl alpha-D-glucopyranoside


organism: Culex quinquefasciatus


organism: Erwinia rhapontici

substrate_name: Maltulose

substrate: Maltulose


substrate_name: Trehalulose

substrate: Trehalulose


substrate_name: Leucrose

substrate: Leucrose


substrate_name: Sucrose

substrate: Sucrose


organism: Bacillus licheniformis

proof_lines: wild-type, kcat/Km: 140000 1/s/M, 37°C, pH 4.5

proof_lines: wild-type, kcat/Km: 770000 1/s/M, 25°C, pH 6.0

proof_lines: wild-type, kcat/Km: 870000 1/s/M, 37°C, pH 5.5


organism: Bacillus sp. dsh19-1

substrate_name: soluble starch

substrate: soluble starch


organism: Bacillus subtilis

substrate_name: H2O; o-Nitrophenyl-alpha-maltoside

substrate: H2O; o-Nitrophenyl-alpha-maltoside


organism: Bifidobacterium longum

proof_lines: recombinant His6-tagged enzyme, pH 5.0, 20°C


organism: Callosobruchus chinensis


organism: Drosophila melanogaster

proof_lines: pH not specified in the publication, 20°C


organism: Euplotes focardii


organism: Geobacillus thermoleovorans

substrate_name: raw starch

proof_lines: pH 4.0, 60°C, recombinant chimeric acidic alpha-amylase Ba-Gt-amy

substrate: raw starch


organism: Glycine max

substrate_name: H2O; Starch

substrate: H2O; Starch


organism: Hordeum vulgare

proof_lines: H395A mutant, pH 5.5, 30°C, kcat/Km: 23 1/s/mg/ml

proof_lines: H395A mutant, pH 5.5, 30°C, kcat/Km: 394 1/s/mg/ml

proof_lines: H395A mutant, kcat below 10 1/s, pH 5.5, 30°C


proof_lines: Y380A/H395A mutant, pH 5.5, 30°C, kcat/Km: 25 1/s/mg/ml

proof_lines: Y380A/H395A mutant, pH 5.5, 30°C, kcat/Km: 543 1/s/mg/ml


organism: Lactobacillus amylovorus


organism: Lactobacillus manihotivorans

substrate_name: H2O; Amylose

substrate: H2O; Amylose


organism: Lactobacillus plantarum

substrate_name: Amylose

substrate: Amylose


organism: Moneuplotes crassus

substrate_name: starch

substrate: starch


organism: Paenibacillus sp. SSG-1

substrate_name: maltopentaose

substrate: maltopentaose


substrate_name: amylose

substrate: amylose


substrate_name: maltose

substrate: maltose


substrate_name: maltoheptaose

substrate: maltoheptaose


substrate_name: maltotriose

substrate: maltotriose


organism: Priestia megaterium

assay_conditions_summary: pH 7.2; 303.15 K; buffer the assay

proof_lines: mutant enzyme A53S, in the presence of 5 mM Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C

proof_lines: mutant enzyme A53S, in the absence of Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C


proof_lines: mutant enzyme H58I, in the presence of 5 mM Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C

proof_lines: mutant enzyme H58I, in the absence of Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C


proof_lines: wild type enzyme, in the presence of 5 mM Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C

proof_lines: wild type enzyme, in the absence of Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C


organism: Pseudoalteromonas haloplanktis

proof_lines: pH not specified in the publication, 5°C

proof_lines: pH not specified in the publication, 25°C


organism: Pyrococcus sp.

substrate_name: glycogen

substrate: glycogen


organism: Ruminococcus bromii

proof_lines: recombinant His-tagged enzyme, pH 5.0, 45°C


organism: Solanum tuberosum

substrate_name: maltotetraose

substrate: maltotetraose


substrate_name: Maltotriose

substrate: Maltotriose


substrate_name: Maltohexaose

substrate: Maltohexaose


organism: Sulfolobus acidocaldarius


substrate_name: maltohexaose

substrate: maltohexaose


substrate_name: 4-nitrophenyl alpha-D-glucopyranoside

substrate: 4-nitrophenyl alpha-D-glucopyranoside


organism: Sulfurisphaera tokodaii


organism: Erwinia rhapontici

substrate_name: Maltitol

substrate: Maltitol


substrate_name: palatinose

substrate: palatinose


substrate_name: Maltose

substrate: Maltose


substrate_name: turanose

substrate: turanose


substrate_name: alpha,alpha-Trehalose

substrate: alpha,alpha-Trehalose


organism: Ferroplasma acidiphilum


substrate_name: sucrose

substrate: sucrose


organism: Mortierella alliacea


substrate_name: maltopentaose

substrate: maltopentaose


substrate_name: H2O; Maltose

substrate: H2O; Maltose


organism: Rattus norvegicus

substrate_name: H2O; p-Nitrophenyl-alpha-D-glucoside

substrate: H2O; p-Nitrophenyl-alpha-D-glucoside


substrate_name: Maltose; H2O

substrate: Maltose; H2O


substrate_name: p-Nitrophenyl-alpha-D-glucoside

substrate: p-Nitrophenyl-alpha-D-glucoside


substrate_name: maltoheptaose

substrate: maltoheptaose


substrate_name: panose

substrate: panose


substrate_name: isomaltose

substrate: isomaltose


substrate_name: maltose

substrate: maltose


substrate_name: maltotetraose

substrate: maltotetraose


organism: Thermoplasma acidophilum


substrate_name: 4-nitrophenyl alpha-D-glucopyranoside

substrate: 4-nitrophenyl alpha-D-glucopyranoside


enzyme_display_name: alpha-galactosidase

organism: Geobacillus stearothermophilus

substrate_name: 4-nitrophenyl alpha-D-galactopyranoside

enzyme: alpha-galactosidase

substrate: 4-nitrophenyl alpha-D-galactopyranoside


assay_conditions_summary: pH 6.5; 298.15 K; buffer 100 mM potassium phosphate

proof_lines: mutant enzyme W336A of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C


substrate_name: raffinose

substrate: raffinose


proof_lines: mutant enzyme W336N of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C


proof_lines: mutant enzyme W336S of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C


proof_lines: wild type enzyme isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C

proof_lines: wild type enzyme isoform AgaB, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C


substrate_name: kojibiose

substrate: kojibiose


substrate_name: maltotriose

substrate: maltotriose


organism: Thermotoga maritima

substrate_name: p-Nitrophenyl-alpha-D-glucoside; H2O

substrate: p-Nitrophenyl-alpha-D-glucoside; H2O


organism: Thermus thermophilus

substrate_name: Isomaltose

substrate: Isomaltose


substrate_name: H2O; alpha,alpha-Trehalose

substrate: H2O; alpha,alpha-Trehalose


substrate_name: alpha,alpha-Trehalose

substrate: alpha,alpha-Trehalose


substrate_name: Maltose

substrate: Maltose


organism: Bacteroides fragilis

substrate_name: p-Nitrophenyl-alpha-D-galactoside

substrate: p-Nitrophenyl-alpha-D-galactoside


substrate_name: H2O; Gal-alpha1->3(Fuc-alpha1->2)Gal

substrate: H2O; Gal-alpha1->3(Fuc-alpha1->2)Gal


organism: Bacteroides thetaiotaomicron

substrate_name: Melibiose

substrate: Melibiose


substrate_name: H2O; p-Nitrophenyl-alpha-D-galactoside

substrate: H2O; p-Nitrophenyl-alpha-D-galactoside


substrate_name: 4-nitrophenyl alpha-D-galactoside

substrate: 4-nitrophenyl alpha-D-galactoside


assay_conditions_summary: pH 6; 308.15 K; buffer 40 mM sodium phosphate

proof_lines: mutant enzyme E470Q, in 40 mM sodium phosphate buffer, pH 6.0, at 35°C


proof_lines: mutant enzyme D415N, in 40 mM sodium phosphate buffer, pH 6.0, at 35°C


proof_lines: wild type enzyme, in 40 mM sodium phosphate buffer, pH 6.0, at 35°C


organism: Bifidobacterium breve


organism: Fusarium sp.


proof_lines:
37°C, pH 5.0, wild-type enzyme
pH 5.0, 65°C, wild-type enzyme


organism: Rhizomucor miehei

substrate_name: stachyose

substrate: stachyose


substrate_name: melibiose

substrate: melibiose


organism: Saccharolobus solfataricus

substrate_name: p-nitrophenyl alpha-D-galactopyranoside

assay_conditions_summary: pH 5; 353.15 K; buffer 50 mM citric acid-Na2HPO4

proof_lines: in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C

substrate: p-nitrophenyl alpha-D-galactopyranoside


organism: Saccharomyces cerevisiae


assay_conditions_summary: pH 7.4; 303.15 K; buffer condition


organism: Saccharopolyspora erythraea


organism: Sphingomonas sp.


organism: Sulfolobus solfataricus


organism: Thermothielavioides terrestris

substrate_name: 4-nitrophenyl alpha-D-galactopyranoside

substrate: 4-nitrophenyl alpha-D-galactopyranoside


substrate_name: 4-O-methylphenyl alpha-D-galactoside

assay_conditions_summary: pH 5; 310.15 K; buffer condition

proof_lines: wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C

substrate: 4-O-methylphenyl alpha-D-galactoside


substrate_name: 4-bromophenyl alpha-D-galactoside

proof_lines: mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37°C

substrate: 4-bromophenyl alpha-D-galactoside


proof_lines: pH 5.0, 65°C, mutant enzyme D327G, assay performed in 500 mM NaN3


organism: Kocuria sp. Mn22


organism: Luteimicrobium xylanilyticum

substrate_name: beechwood xylan

substrate: beechwood xylan


organism: Malbranchea cinnamomea

substrate_name: oat spelt xylan

substrate: oat spelt xylan


organism: Massilia sp. RBM26


organism: Neocallimastix patriciarum

assay_conditions_summary: pH 6; 323.15 K; buffer notes) and the accession is annotated bifunctional

proof_lines: in 12 mM sodium citrate, 50 mM sodium phosphate buffer (pH 6.5)


organism: Nesterenkonia xinjiangensis

proof_lines: pH 7.0, 55°C, recombinant enzyme, birchwood xylan

proof_lines: pH 7.0, 55°C, recombinant enzyme, oat spelt xylan


organism: Niallia circulans

proof_lines: recombinant wild-type enzyme, pH 7.2, 22°C


organism: Penicillium citrinum

substrate_name: birchwood xylan

substrate: birchwood xylan


organism: Penicillium sp. CGMCC 1669


organism: Pseudoalteromonas haloplanktis

substrate_name: xylan

substrate: xylan


organism: Pseudopedobacter saltans

proof_lines: His-tagged enzyme, pH 6.0, 40°C, substrate xylan MW 20000-30000


organism: Teredinibacter turnerae


organism: Thermoanaerobacterium saccharolyticum


organism: Thermochaetoides thermophila

proof_lines: recombinant His-tagged enzyme, pH 5.0, 55°C


organism: Thermomyces lanuginosus


organism: Thermothelomyces thermophilus

substrate_name: Remazol brilliant blue R 4-O-methyl-D-glucuronoxylan; H2O


substrate: Remazol brilliant blue R 4-O-methyl-D-glucuronoxylan; H2O


organism: Thermotoga maritima


proof_lines: mutant enzyme N110D/Q111Y, at pH 5.0 and 80°C


organism: Trichoderma reesei

proof_lines:
pH 5.0, 50°C, birchwood xylan
pH 5.0, 50°C, beechwood xylan
pH 5.0, 50°C, oat spelt xylan


organism: uncultured bacterium

substrate_name: oat-spelt xylan

substrate: oat-spelt xylan


proof_lines:
Raw SKiD Env: Temp=65°C, pH=5
Raw SKiD Env: Temp=37°C, pH=5


organism: Thermus sp. T2


organism: Alicyclobacillus sp.

substrate_name: xylotetraose

substrate: xylotetraose


organism: Aspergillus sulphureus

proof_lines: recombinant wild-type enzyme, pH 5.0, 50°C, oat spelt xylan


organism: Bacillus circulans

substrate_name: H2O; 1,4-beta-D-Xylan

unit: l/(min*g)

substrate: H2O; 1,4-beta-D-Xylan


organism: Bacillus subtilis


proof_lines: chimera carrying a carbohydrate-binding module, pH 6.0, 50°C


proof_lines:
wild-type, pH 6.0, 50°C
wild-type


organism: Bacillus thermodenitrificans

substrate_name: 1,4-beta-D-Xylan; H2O

substrate: 1,4-beta-D-Xylan; H2O


organism: Cellvibrio japonicus

substrate_name: H2O; 4-Nitrophenyl-beta-D-xylobioside

substrate: H2O; 4-Nitrophenyl-beta-D-xylobioside


organism: Fusarium oxysporum f. sp. vasinfectum

proof_lines:
pH 8.0, 40°C
Raw SKiD Env: Temp=40°C, pH=8


organism: Geobacillus stearothermophilus


organism: Hordeum vulgare

assay_conditions_summary: pH 5; 313.15 K; buffer citric acid and disodium hydrogen phosphate

proof_lines: in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C


organism: Pseudothermotoga thermarum


organism: Rhizophlyctis rosea

proof_lines: pH 7, 50°C, large enzyme form RrXyn11A L

proof_lines: pH 7, 40°C, small enzyme form RrXyn11A S


organism: Rhodothermaceae bacterium RA

assay_conditions_summary: pH 8


organism: Scheffersomyces stipitis


organism: Sphingobacterium sp. TN19


organism: Streptomyces fradiae


organism: Streptomyces sp. TN119


organism: Talaromyces funiculosus


proof_lines: apparent value for the recombinant enzyme


organism: Talaromyces pinophilus

substrate_name: wheat arabinoxylan

substrate: wheat arabinoxylan


organism: Teredinibacter turnerae

substrate_name: marine beta-1,3-beta-1,4-xylan

proof_lines: pH 6.0, 37°C, recombinant enzyme mutant D281N

substrate: marine beta-1,3-beta-1,4-xylan


substrate_name: arabinoxylan

proof_lines: pH 6.0, 37°C, recombinant wild-type enzyme

substrate: arabinoxylan


proof_lines: mutant enzyme W336F of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C


proof_lines: mutant enzyme W336D of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25°C


substrate_name: raffinose

substrate: raffinose


substrate_name: 4-nitrophenyl alpha-D-galactopyranoside

substrate: 4-nitrophenyl alpha-D-galactopyranoside


organism: Homo sapiens


organism: Mus musculus


organism: Phanerodontia chrysosporium

substrate_name: 1-naphthyl alpha-D-galactoside

assay_conditions_summary: pH 5

proof_lines: temperature not specified in the publication, pH 5

substrate: 1-naphthyl alpha-D-galactoside


organism: Pseudoalteromonas sp. KMM 701

substrate_name: Unknown

substrate: Unknown


organism: Rhizomucor miehei


substrate_name: melibiose

substrate: melibiose


enzyme_display_name: subtilisin

organism: Bacillus lentus

substrate_name: Peptidyl-p-nitroanilide

enzyme: subtilisin

substrate: Peptidyl-p-nitroanilide


organism: Bacillus amyloliquefaciens

substrate_name: N-Acetyl-L-tyrosine methyl ester

substrate: N-Acetyl-L-tyrosine methyl ester


substrate_name: N-Acetyl-L-tryptophan methyl ester

substrate: N-Acetyl-L-tryptophan methyl ester


substrate_name: Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide

substrate: Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide


enzyme_display_name: peptidyl-dipeptidase A

organism: Oryctolagus cuniculus

substrate_name: Hip-His-Leu

assay_conditions_summary: pH 8.3; 310.15 K; buffer sodium borate

enzyme: peptidyl-dipeptidase A

substrate: Hip-His-Leu


substrate_name: Angiotensin I

substrate: Angiotensin I


substrate_name: Angiotensin I; H2O

proof_lines: pH 7.5, temperature not specified in the publication

proof_lines: pH and temperature not specified in the publication

substrate: Angiotensin I; H2O


organism: Struthio camelus australis


substrate_name: N-Acetyl-L-phenylalanine methyl ester

substrate: N-Acetyl-L-phenylalanine methyl ester


organism: uncultured bacterium

substrate_name: oat-spelt xylan

substrate: oat-spelt xylan


substrate_name: beechwood xylan

proof_lines:
mutant XylA, pH 6.0, 50°C
mutant XylB, pH 6.0, 50°C

substrate: beechwood xylan


organism: Drosophila melanogaster


substrate_name: Hip-His-Leu; H2O

proof_lines: C-catalytic domain of ACE, pH 7.5, temperature not specified in the publication

substrate: Hip-His-Leu; H2O


substrate_name: N-Acetyl-Ser-Asp-Lys-Pro

substrate: N-Acetyl-Ser-Asp-Lys-Pro


substrate_name: Leu-enkephalin

substrate: Leu-enkephalin


substrate_name: Leu-enkephalinamide

substrate: Leu-enkephalinamide


substrate_name: H2O; Hip-His-Leu

substrate: H2O; Hip-His-Leu


substrate_name: Peptidyl-p-nitroanilide; H2O

substrate: Peptidyl-p-nitroanilide; H2O


organism: Bacillus licheniformis


substrate_name: N-Acetyl-L-tryptophan methyl ester; H2O

substrate: N-Acetyl-L-tryptophan methyl ester; H2O


organism: Bacillus subtilis


substrate_name: furanacryloyl-L-Phe-Gly-Gly

substrate: furanacryloyl-L-Phe-Gly-Gly


proof_lines: 37°C, pH 8.3, chimeric enzyme C417-579Ndom-ACE


substrate_name: Succinyl-Ala-Ala-Pro-Ala-p-nitroanilide

substrate: Succinyl-Ala-Ala-Pro-Ala-p-nitroanilide


substrate_name: Succinyl-Ala-Ala-Pro-Met-p-nitroanilide

substrate: Succinyl-Ala-Ala-Pro-Met-p-nitroanilide


substrate_name: Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide

substrate: Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide


organism: Plasmodium falciparum

substrate_name: Ac-LVSADNIDIS; H2O

substrate: Ac-LVSADNIDIS; H2O


enzyme_display_name: arginase

substrate_name: L-arginine

enzyme: arginase

substrate: L-arginine


substrate_name: L-Arginine; H2O

substrate: L-Arginine; H2O


proof_lines: mutant S230G, activated by Co2+, pH 7.4, 37°C

proof_lines: mutant S230G, activated by Mn2+, pH 7.4, 37°C

proof_lines: product inhibition constant, Mn2+-activated mutant S230G, pH 7.4, 37°C


proof_lines: mutant S230C, activated by Mn2+, pH 7.4, 37°C

proof_lines: mutant S230C, activated by Co2+, pH 7.4, 37°C


proof_lines: mutant D181N/S230G, activated by Co2+, pH 7.4, 37°C

proof_lines: mutant D181N/S230G, activated by Mn2+, pH 7.4, 37°C

proof_lines: product inhibition constant, Co2+-activated mutant D181N/S230G, pH 7.4, 37°C


proof_lines: mutant D181S, activated by Co2+, pH 7.4, 37°C


proof_lines: mutant D181S/S230G, activated by Co2+, pH 7.4, 37°C


proof_lines: mutant S230T, activated by Co2+, pH 7.4, 37°C


proof_lines: mutant S230D, activated by Co2+, pH 7.4, 37°C


proof_lines: mutant D181E/S230A, activated by Co2+, pH 7.4, 37°C


proof_lines: mutant D181E, activated by Co2+, pH 7.4, 37°C


proof_lines: mutant D181N, activated by Mn2+, pH 7.4, 37°C

proof_lines: mutant D181N, activated by Co2+, pH 7.4, 37°C

proof_lines: product inhibition constant, Co2+-activated mutant D181N, pH 7.4, 37°C


assay_conditions_summary: pH 8; 298.15 K; buffer phosphate


proof_lines:
recombinant enzyme, 5°C, pH 8.6
recombinant enzyme, 45°C, pH 8.6
recombinant enzyme, 25°C, pH 8.6


proof_lines: arginase 2, pH and temperature not specified in the publication


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme N130D/S137CN139D

proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme N130D/S137C/N139D


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme D181T


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme N130D/N139D


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme N130D


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme I129T/N130Y/T131A


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme V182E


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme S137C


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme D181T/V182E


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme S137C/N139D


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme N139D


proof_lines: pH 9.5, temperature not specified in the publication, mutant enzyme N130D/S137C


organism: Bacillus brevis

substrate_name: H2O; L-Arginine

substrate: H2O; L-Arginine


organism: Bacillus caldovelox


organism: [Bacillus] caldovelox


proof_lines:
at pH 8.4 and 45°C
Raw SKiD Env: Temp=45°C, pH=8.4


organism: Bos taurus


organism: Rattus norvegicus


proof_lines: T135S mutant, kcat is unchanged relatively to values measured for the wild-type enzyme

proof_lines: T135A mutant, is reduced 26% relative to values measured for the wild-type enzyme


substrate_name: Unknown

substrate: Unknown


organism: Saccharomyces cerevisiae


organism: Schistosoma mansoni


organism: Solanum lycopersicum


organism: Sulfobacillus acidophilus


organism: Sus scrofa


organism: Geobacillus thermodenitrificans


organism: Glaciozyma antarctica


organism: Helicobacter pylori


organism: Heteropneustes fossilis


organism: Homo sapiens


proof_lines: pH 9.5, temperature not specified in the publication, wild-type enzyme


organism: Plasmodium falciparum 3D7

proof_lines: mutant E295A/R404A, pH 8.0, temperature not specified in the publication


proof_lines: mutant E295R, pH 8.0, temperature not specified in the publication


proof_lines: mutant R404A, pH 8.0, temperature not specified in the publication


proof_lines: wild-type, pH 8.0, temperature not specified in the publication


proof_lines:
mutant dN-PFA, pH 7.4, 37°C
mutant dN-PFA, pH 8.0, 37°C
mutant dN-PFA, pH 8.5, 37°C


proof_lines:
Raw SKiD Env: Temp=37°C, pH=7.4
Raw SKiD Env: Temp=37°C, pH=8
Raw SKiD Env: Temp=37°C, pH=8.5


assay_conditions_summary: pH 6


organism: Leishmania amazonensis


organism: Leishmania donovani


organism: Leishmania infantum


organism: Leishmania mexicana


organism: Mus musculus


organism: Neurospora crassa


organism: Oryza sativa


organism: Plasmodium falciparum


proof_lines: mutant E295A, pH 8.0, temperature not specified in the publication


enzyme_display_name: orotidine-5'-phosphate decarboxylase

organism: Methanothermobacter thermautotrophicus

substrate_name: orotidine 5'-phosphate

proof_lines:
above 10 mM
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=25°C, pH=7.1

enzyme: orotidine-5'-phosphate decarboxylase

substrate: orotidine 5'-phosphate


organism: Xenopus laevis


organism: Escherichia coli

substrate_name: Orotidine 5'-phosphate

substrate: Orotidine 5'-phosphate


organism: Methanobacterium thermoautotrophicus


enzyme_display_name: dihydrolipoyl dehydrogenase

organism: Unknown

substrate_name: H2O; 2-Oxoglutarate; Ferricyanide

assay_conditions_summary: cofactor NAD+

enzyme: dihydrolipoyl dehydrogenase

substrate: H2O; 2-Oxoglutarate; Ferricyanide


substrate_name: NAD+; Dihydrolipoamide

assay_conditions_summary: cofactor NADP+

substrate: NAD+; Dihydrolipoamide


organism: Lupinus angustifolius

proof_lines:
isozyme AAT-P1, pH 7.6, 25°C
isozyme AAT-P2, pH 7.6, 25°C


organism: Medicago sativa

substrate_name: oxaloacetate

proof_lines: isozyme AAT-2, including forms 2a, 2b and 2c, pH 8.0

substrate: oxaloacetate


organism: Oryctolagus cuniculus

proof_lines:
cytosolic isozyme, pH 8.0, 37°C
mitochondrial isozyme, pH 8.0, 37°C


substrate_name: L-aspartate

proof_lines:
isoenzyme AAT-1, pH 7.8, 37°C
isoenzyme AAT-2
isoenzyme AAT-2, pH 7.8, 37°C

substrate: L-aspartate


organism: Ovis aries

proof_lines:
pH 7.4, 25°C
mitochondrial isozyme, pH 7.4, 25°C
cytosolic isozyme, pH 7.4, 25°C