organism: Aspergillus niger

assay_conditions_summary: pH 5.5; 310.15 K; buffer 0.2 M citrate

proof_lines: mutant enzyme A58E/Q191R/T271R, at 37°C, in 0.2 M citrate buffer, pH 5.5


substrate_name: myo-inositol hexakisphosphate

source_db: brenda

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: mutant enzyme A58E/P65S, at 37°C, in 0.2 M citrate buffer, pH 5.5

substrate: myo-inositol hexakisphosphate

source: brenda


proof_lines: mutant enzyme A58E/Q191R, at 37°C, in 0.2 M citrate buffer, pH 5.5


proof_lines: mutant enzyme A58E/P65S/Q191R/T271R, at 37°C, in 0.2 M citrate buffer, pH 5.5


assay_conditions_summary: pH 4

proof_lines: mutant enzyme A58E/P65S/Q191R/T271R/E228K, at pH 4.0

proof_lines: mutant enzyme A58E/P65S/Q191R/T271R/E228K, at pH 5.5


proof_lines: mutant enzyme A58E/P65S/Q191R/T271R/E228K/S149P, at pH 4.0

proof_lines: mutant enzyme A58E/P65S/Q191R/T271R/E228K/S149P, at pH 5.5


mutation_signature: Unknown

assay_conditions_summary: pH 3.5; 310.15 K; cofactor ADP

proof_lines: pH 2.5, 55°C. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate h


mutation_type: substitution


sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

assay_conditions_summary: pH 5; 310.15 K; buffer 0.2 M citrate

proof_lines: purified recombinant His-tagged wild-type enzyme, pH 5.0, 37°C

proof_lines: wild type enzyme, at 37°C, in 0.2 M citrate buffer, pH 5.5


proof_lines: purified recombinant His-tagged mutant K41E, pH 5.0, 37°C


proof_lines: purified recombinant His-tagged mutant E121F, pH 5.0, 37°C


organism: Bacillus amyloliquefaciens

substrate_name: phytate

substrate: phytate


substrate_name: Unknown

km_display: Not reported

ki_unit: mM

proof_lines: free, not complexed with Ca2+, competitive inhibitor

proof_lines: free, not complexed with phytate, competitive inhibitor

substrate: Unknown


organism: Yersinia enterocolitica

protein_accession_database: UniProt

sequence_source: UniProtKB REST direct accession lookup for D9D7K9

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: recombinant wild-type enzyme, pH 4.5, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB REST direct accession lookup for D9D7K9 plus direct R79G substitution reconstruction and applied mutation signature R79G to reconstruct the assayed variant sequence.

proof_lines: recombinant enzyme mutant R79G, pH 4.5, 37°C


organism: Yersinia mollaretii

substrate_name: 4-methylumbelliferyl phosphate

sequence_source: UniProtKB REST direct accession lookup for C4S833

proof_lines: recombinant wild-type enzyme, pH 6.6, 37°C

substrate: 4-methylumbelliferyl phosphate


sequence_variant_note: Resolved canonical sequence from UniProtKB REST direct accession lookup for C4S833 plus direct T44V/K45E substitution reconstruction and applied mutation signature T44V/K45E to reconstruct the assayed variant sequence.

proof_lines: recombinant mutant T44V/K45E, pH 6.6, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB REST direct accession lookup for C4S833 plus direct T44I/K45E substitution reconstruction and applied mutation signature T44I/K45E to reconstruct the assayed variant sequence.

proof_lines: recombinant mutant T44I/K45E, pH 6.6, 37°C


organism: Yersinia pestis


organism: Yersinia rohdei


organism: Zea mays


organism: Citrobacter freundii


organism: Citrobacter gillenii


organism: Corylus avellana

assay_conditions_summary: pH 5; cofactor ATP


organism: Cronobacter turicensis


organism: Debaryomyces castellii

substrate_name: p-nitrophenyl phosphate

assay_conditions_summary: pH 4; 310.15 K; buffer notes '200 mM sodium acetate buffer

proof_lines: in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C

proof_lines: in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0 at 37°C

substrate: p-nitrophenyl phosphate


organism: Dendroctonus frontalis

proof_lines:
recombinant enzyme, pH 3.9, 37°C
recombinant enzyme, pH 3.9, 52.5°C


organism: Enterobacter sp. ACSS


organism: Escherichia coli

proof_lines: 37°C, pH 3.5, enzyme expressed in Pichia pastoris (pGAPZalphaA). The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphos

proof_lines: 37°C, pH 5.5, enzyme expressed in Pichia pastoris (pGAPZalphaA) or Pichia pastoris (pPICZalphaA). The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. my


organism: Bacillus cereus


organism: Bacillus licheniformis


organism: Bacillus sp. (in: firmicutes)

proof_lines: beta-propeller phytase 168PhyA, pH 7.0, 55°C


organism: Bacillus sp. SJ-10

proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


organism: Bacillus sp. SJ-48


organism: Bacillus subtilis

proof_lines:
at pH 6.5
Bacillus subtilis var. natto
pH 7.0, 37°C


organism: Citrobacter braakii


organism: Papiliotrema laurentii

substrate_name: di-sodium phenyl phosphate dihydrate

assay_conditions_summary: pH 5; 335.15 K; buffer 50 mM sodium acetate; cofactor ATP

proof_lines: at 62°C, in 50 mM sodium acetate buffer (pH 5)

substrate: di-sodium phenyl phosphate dihydrate


organism: Pectobacterium carotovorum


organism: Pectobacterium wasabiae


organism: Penicillium parvum

proof_lines:
calcium salt, pH 5.5, 50°C
pH 5.5, 50°C
sodium salt, pH 5.5, 50°C


organism: Pseudomonas sp.


organism: Pseudomonas sp. FB15

proof_lines: chimera of the phytase of Bacillus sp. SJ-10 fused with the N-terminal domain of the Pseudomonas sp. FB15 enzyme, pH not specified in the publication, temperature not specified in the publication

proof_lines: chimera of the phytase of Bacillus sp. SJ-48 fused with the N-terminal domain of the Pseudomonas sp. FB15 enzyme, pH not specified in the publication, temperature not specified in the publication


organism: Pseudomonas syringae


organism: Raoultella terrigena

substrate_name: ATP

assay_conditions_summary: cofactor NADP+

substrate: ATP


organism: Rhizopus microsporus

substrate_name: 4-nitrophenyl phosphate

substrate: 4-nitrophenyl phosphate


organism: Rhodotorula mucilaginosa


organism: Saccharomyces sp.


organism: Scheffersomyces spartinae


organism: Penicillium oxalicum

sequence_source: UniProtKB:Q6YNE9; active direct lookup on 2026-06-19


proof_lines: pH 6.0, 40°C, recombinant His-tagged C-terminal domain

proof_lines: pH 6.0, 40°C, recombinant His-tagged full-length enzyme


organism: Serratia sp. (in: enterobacteria)

sequence_source: UniProtKB:G4WZU3; active direct lookup on 2026-06-19


sequence_source: UniProtKB:G4WZU4; active direct lookup on 2026-06-19


organism: Shigella sp. CD2

proof_lines: pH 5.5, 37°C, recombinant glycosylated enzyme expressed from Pichia pastoris

proof_lines: pH 5.5, 37°C, recombinant nonglycosylated enzyme expressed from Escherichia coli


organism: Streptomyces sp.


organism: Thermothelomyces heterothallicus


organism: Thermothelomyces thermophilus

sequence_source: UniProtKB:G2QH47; active direct lookup on 2026-06-19


sequence_source: UniProtKB:O00107; active direct lookup on 2026-06-19

proof_lines:
recombinant enzyme, pH 5.0, 60°C
native enzyme, pH 5.0, 60°C


organism: Triticum aestivum

sequence_source: UniProtKB:C4PKK7; active direct lookup on 2026-06-19; context only because EC annotation conflicts


sequence_source: UniProtKB:C4PKK9; active direct lookup on 2026-06-19; context only because EC annotation conflicts


organism: Wickerhamomyces anomalus


organism: Xylanibacter ruminicola

proof_lines: pH 5.0, 55°C, recombinant His-tagged enzyme


organism: Yersinia enterocolitica

sequence_variant_note: Resolved canonical sequence from UniProtKB:D9D7K9; active direct lookup on 2026-06-19 and applied mutation signature R79S to reconstruct the assayed variant sequence.

proof_lines: recombinant enzyme mutant R79S, pH 4.5, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:D9D7K9; active direct lookup on 2026-06-19 and applied mutation signature R79L to reconstruct the assayed variant sequence.

proof_lines: recombinant enzyme mutant R79L, pH 4.5, 37°C


organism: Escherichia coli


organism: Evernia prunastri


organism: Glycine max

assay_conditions_summary: cofactor ATP


organism: Klebsiella aerogenes

proof_lines:
high molecular weight form
low molecular weight form


organism: Klebsiella sp.


organism: Kodamaea ohmeri

proof_lines: 5.0 mM myo-inositol hexakisphosphate in 0.2 M sodium acetate, pH 5.0, at 60°C


organism: Loigolactobacillus coryniformis


organism: Lupinus albus

substrate_name: GTP

proof_lines:
pH 5.0, 35°C, phytase LP2
pH 5.0, 35°C, phytase LP12
pH 5.0, 35°C, phytase LP11

substrate: GTP


organism: Macrophomina phaseolina


organism: Nicotiana tabacum

assay_conditions_summary: pH 5; 310.15 K; buffer 50 mM NaOAc (pH 5.0) or 100 mM Tris-HCl

proof_lines: in 50 mM NaOAc (pH 5.0) or 100 mM Tris-HCl buffer (pH 8.0), at 37°C


organism: Pantoea agglomerans

substrate_name: D-ribose 5-phosphate

substrate: D-ribose 5-phosphate


organism: Schizophyllum commune


organism: Schwanniomyces occidentalis


organism: Thermothelomyces heterothallicus

proof_lines: substrate potassium phytate, pH 5.0, 60°C, recombinant enzyme

proof_lines: substrate calcium phytate, pH 5.0, 60°C, recombinant enzyme

proof_lines: substrate sodium phytate, pH 5.0, 60°C, recombinant enzyme


organism: uncultured bacterium


proof_lines: recombinant wild-type enzyme, pH 2.5, 40°C


organism: Yersinia kristensenii


enzyme_display_name: HIV-1 retropepsin

organism: Unknown

enzyme: HIV-1 retropepsin


organism: Human immunodeficiency virus 1

proof_lines: pH 5.0, 25°C, mutant enzyme Q7K/L33I/L63I/C67A/C95A

proof_lines: pH 5.0, 25°C, truncated version PR-(5-99) from mutant enzyme Q7K/L33I/L63I/C67A/C95A


proof_lines: pH 5.0, 25°C, mutant enzyme Q7K/R8Q/L33I/L63I/C67A/C95A


proof_lines: pH 4.7, 37°C, recombinant V6 mutant M46I


proof_lines: pH 4.7, 37°C, recombinant V6 mutant M46I/I84V


proof_lines: pH 4.7, 37°C, recombinant V6 mutant M46I/I54V/I84V


proof_lines: pH 4.7, 37°C, recombinant V6 mutant I54V/I84V


proof_lines: 37°C, pH 4.7, mutant enzyme D30N/L63PA71V/L90M


proof_lines: 37°C, pH 4.7, HIV-1 subtype B protease mutant N88D


substrate_name: VSQNYPIVQ

substrate: VSQNYPIVQ


substrate_name: PARVLAEAMR

proof_lines:
mutant enzyme G48V/L90M
pH 5.0, 37°C, mutant enzyme G48V/L90M

substrate: PARVLAEAMR


substrate_name: PARVLIEAMR

substrate: PARVLIEAMR


proof_lines:
mutant enzyme I84V
pH 5.0, 37°C, mutant enzyme I84V
pH 5.0, mutant I84V


substrate_name: PARVLVEAMR

proof_lines:
wild-type enzyme
wild-type, pH 5.6, 26°C
pH 5.0, 37°C, wild-type enzyme

substrate: PARVLVEAMR


substrate_name: PARVLFEAMR

substrate: PARVLFEAMR


proof_lines: 37°C, pH 4.7, HIV-1 subtype B protease mutant D30N

proof_lines: 37°C, pH 4.7, HIV-1 subtype C protease mutant D30N


proof_lines: 37°C, pH 4.7, HIV-1 subtype B protease D30N/L90M


proof_lines: 37°C, pH 4.7, HIV-1 subtype B protease mutant L90M


proof_lines: 37°C, pH 4.7, HIV-1 subtype B protease N88D/L90M


proof_lines: 37°C, pH 4.7, HIV-1 subtype B protease mutant D30M/L90M

proof_lines: 37°C, pH 4.7, HIV-1 subtype B protease mutant 0.03 D30M/L90M


proof_lines:
37°C, pH 4.7, HIV-1 subtype B protease
pH 6.0, 25°C
pH 4.8, 0.1 M NaCl


proof_lines: 37°C, pH 4.7, HIV-1 subtype B protease D30N/N88D

proof_lines: 37°C, pH 4.7, HIV-1 subtype B protease mutant D30N/N88D


proof_lines:
mutant I50V, pH 5.6, 26°C
pH 5.0, 25°C, mutant I50V
mutant I50V, pH 5.5


proof_lines: pH 4.7, 37°C, recombinant V6 mutant I54V


substrate_name: AETFYVDGAA

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

proof_lines: pH 5.6, 37°C, autolysis resistant enzyme form PR with the mutations Q7K/L33I/L63I/C67A/C95A

proof_lines: pH 5.6, 37°C, autolysis reistant and truncated enzyme form PR-(5-99) with mutation Q7K/L33I/L63I/C67A/C95A

substrate: AETFYVDGAA


proof_lines: mutant enzyme L10I/L63P/A71V/G73S/I84V/L90M, in 0.1 M sodium acetate, 1 M sodium chloride, 1 mM EDTA, 1 mM dithiothreitol, 2% (v/v) DMSO, and 1 mg/ml bovine serum albumin, at pH 4.7 and 22°C


sequence_source: UniProt P03369 Protease chain 491..589, REST lookup 2026-06-19

proof_lines: wild type enzyme, in 0.1 M sodium acetate, 1 M sodium chloride, 1 mM EDTA, 1 mM dithiothreitol, 2% (v/v) DMSO, and 1 mg/ml bovine serum albumin, at pH 4.7 and 22°C


substrate_name: IPFAAAQQRK

sequence_source: UniProt Q7SSE3 full Protease entry, REST lookup 2026-06-19

assay_conditions_summary: pH 5.6; 310.15 K; buffer 0.5 M potassium phosphate

proof_lines: wild type enzyme, in 0.5 M potassium phosphate buffer, pH 5.6, containing 10% (v/v) glycerol, 2 mM EDTA, 10 mM dithiothreitol, 4 M NaCl, at 37°C

substrate: IPFAAAQQRK


substrate_name: VLQNYPIVQ

proof_lines: mutant enzyme V32I/I47V/V82I, in 0.5 M potassium phosphate buffer, pH 5.6, containing 10% (v/v) glycerol, 2 mM EDTA, 10 mM dithiothreitol, 4 M NaCl, at 37°C

substrate: VLQNYPIVQ


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt Q7SSI0 full Protease entry using V32I/I47V/V82I, REST lookup 2026-06-19 and applied mutation signature V32I/I47V/V82I to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme V32I/I47V/V82I, at pH 4.0 and 28°C

proof_lines: mutant enzyme V32I/I47V/V82I, at pH 5.8 and 28°C


sequence_source: UniProt Q7SSI0 full Protease entry, REST lookup 2026-06-19


proof_lines: pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant enzyme PRV82A, at pH 5.6 and 37°C


sequence_source: UniProt Q5RZ08 full Protease entry, REST lookup 2026-06-19


sequence_source: UniProt P12497 Protease chain 489..587, REST lookup 2026-06-19

proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt P12497 Protease chain 489..587 using I50V/A71V, REST lookup 2026-06-19 and applied mutation signature I50V/A71V to reconstruct the assayed variant sequence.

proof_lines: mutant I50V/A71V, pH not specified in the publication, temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProt P12497 Protease chain 489..587 using I84V, REST lookup 2026-06-19 and applied mutation signature I84V to reconstruct the assayed variant sequence.

proof_lines: mutant I84V, pH not specified in the publication, temperature not specified in the publication


substrate_name: DTVIEEMS

proof_lines: pH 5.5, 30°C, mutant enzyme Q7K/L33I/L63I

substrate: DTVIEEMS


proof_lines: pH 5.0, 25°C, mutant enzyme C67A/C95A and mutant enzyme C95A


substrate_name: TATIMMQRGN

substrate: TATIMMQRGN


proof_lines:
pH 5.0, 37°C, mutant enzyme G48V
mutant G48V, pH 5.6, 26°C


proof_lines:
pH 5.0, 37°C, mutant enzyme V82A
pH 5.0, mutant V82A
mutant V82A enzyme


enzyme_display_name: glucuronosyltransferase

organism: Homo sapiens

substrate_name: scopoletin

proof_lines:
wild-type
wild-type isozyme UGT1A6
wild-type, pH 7.4, 37°C

enzyme: glucuronosyltransferase

substrate: scopoletin


substrate_name: morphine

proof_lines: membrane-bound recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C

proof_lines: membrane-bound recombinant isoform UGT1A10 from HEK-293 cells, at pH 7.4 and 37°C

proof_lines: without phospholipid purified recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C

substrate: morphine


organism: Bos taurus

proof_lines:
alveolar macrophages
liver
lung


organism: Bubo virginianus

substrate_name: beta-estradiol

substrate: beta-estradiol


organism: Canis lupus familiaris

substrate_name: denopamine

proof_lines: liver microsomes, substrate denopamine, formation of G2 product, pH 7.5, 37°C

proof_lines: liver microsomes, substrate denopamine, formation of G1 product, pH 7.5, 37°C

substrate: denopamine


organism: Felis catus

substrate_name: UDPglucuronate

proof_lines: 0.017 mM bilirubin monoglucuronide added, at pH 6.5

proof_lines: 0.016 mM bilirubin monoglucuronide added, at pH 8.1

substrate: UDPglucuronate


organism: Gallus gallus


organism: Haliaeetus albicilla


substrate_name: UDP-glucuronate

substrate: UDP-glucuronate


proof_lines: formation of dopamine-3-O-glucuronide, mutant F90A

proof_lines: formation of dopamine-4-O-glucuronide, mutant F90A


proof_lines: formation of dopamine-4-O-glucuronide, mutant F93A

proof_lines: formation of dopamine-3-O-glucuronide, mutant F93A


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed accession P19224 (UGT1A6_HUMAN) plus H371A residue substitution reconstructed during review and applied mutation signature H371A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed accession O60656 (UGT1A9_HUMAN) plus H369A residue substitution reconstructed during review and applied mutation signature H369A to reconstruct the assayed variant sequence.


substrate_name: zidovudine

proof_lines: pH 7.4, 37°C, recombinant dimer of CFP-tagged A71S and HA-tagged H268Y mutant monomers

substrate: zidovudine


substrate_name: xanthotoxol

proof_lines: isozyme UGT1A10, Michaelis-Menten kinetic, pH 7.4, 37°C

substrate: xanthotoxol


verification_status: unverified

proof_lines: with UDP-D-glucose, recombinant enzyme in microsomes, pH 7.4, 37°C

proof_lines: with UDP-D-xylose, recombinant enzyme in microsomes, pH 7.4, 37°C

status: unverified


substrate_name: propofol

proof_lines: UGT1A9/UGT2B7(mutant H35A)-CFP heterodimer, pH 7.4, 37°C

substrate: propofol


mutation_type: deletion

proof_lines: UGT1A9/UGT2B7(mutant DELTA N1-23)-CFP heterodimer, pH 7.4, 37°C


proof_lines: UGT1A9/UGT2B7(mutant DELTA C525-529)-CFP heterodimer, pH 7.4, 37°C


proof_lines: UGT1A9/UGT2B7(mutant H268Y)-CFP heterodimer, pH 7.4, 37°C

proof_lines: pH 7.4, 37°C, recombinant HA-tagged mutant H268Y dimer

proof_lines: pH 7.4, 37°C, recombinant CFP-tagged mutant H268Y dimer


proof_lines: UGT1A9/UGT2B7(mutant DELTA C493-529)-CFP heterodimer, pH 7.4, 37°C


proof_lines: UGT1A9/UGT2B7(mutant DELTA C511-529)-CFP heterodimer, pH 7.4, 37°C


proof_lines: UGT1A9/UGT2B7(mutant N68A/N315A)-CFP heterodimer, pH 7.4, 37°C


proof_lines: pH 7.4, 37°C, recombinant UGT1A6, substrate inhibition model


organism: Macaca fascicularis

proof_lines: recombinant isoform UGT1A1, pH 7.4, 37°C


substrate_name: hyodeoxycholic acid

sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed accession P16662 via REST accessions endpoint; checked 2026-06-19. and applied mutation signature Y33F to reconstruct the assayed variant sequence.

substrate: hyodeoxycholic acid


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed accession P06133 via REST accessions endpoint; checked 2026-06-19. and applied mutation signature F33L to reconstruct the assayed variant sequence.


sequence_source: UniProtKB reviewed accession P16662 via REST accessions endpoint; checked 2026-06-19.

proof_lines: isozyme UGT2B7 in COS-1 cell microsomes, pH 7.8, 37°C, in presence with CYP2C9


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed accession P06133 via REST accessions endpoint; checked 2026-06-19. and applied mutation signature F33Y to reconstruct the assayed variant sequence.


sequence_source: UniProtKB reviewed accession Q9HAW7 via REST accessions endpoint; checked 2026-06-19.

proof_lines: isozyme UGT1A7, Michaelis-Menten kinetic, pH 7.4, 37°C


sequence_source: UniProtKB reviewed accession P54855 via REST accessions endpoint; checked 2026-06-19.

proof_lines:
pH 7.4, 37°C, isozyme UGT2B15
37°C, pH 7.4
isoform UGT2B15, at pH 7.4 and 37°C


sequence_source: UniProtKB reviewed accession O75795 via REST accessions endpoint; checked 2026-06-19.

proof_lines:
pH 7.4, 37°C, isozyme UGT2B17
37°C, pH 7.5
37°C, pH 7.4


sequence_source: UniProtKB reviewed accession Q9HAW9 via REST accessions endpoint; checked 2026-06-19.

proof_lines: pH 7.4, temperature not specified in the publication, isozyme UGT1A8


substrate_name: UDP-D-glucuronate

sequence_source: UniProtKB reviewed accession P19224 via REST accessions endpoint; checked 2026-06-19.

proof_lines: pH 7.4, 37°C, recombinant wild-type isozyme UGT1A6

substrate: UDP-D-glucuronate


proof_lines:
recombinant genetic variant UGT2A3.2
recombinant genetic variant UGT2A3.1


substrate_name: diphenhydramine

sequence_source: UniProtKB reviewed accession P36537 via REST accessions endpoint; checked 2026-06-19.

proof_lines: pH 7.4, 37°C, recombinant isozyme UGT2B10

substrate: diphenhydramine


substrate_name: imipramine

sequence_source: UniProtKB reviewed accession P22310 via REST accessions endpoint; checked 2026-06-19.

proof_lines: imipramine N-glucuronide formation, 1A4 co-expressed with isoform 1A4

proof_lines: imipramine N-glucuronide formation, 1A4 co-expressed with isoform 1A6

substrate: imipramine


proof_lines: pH 7.4, 37°C, recombinant dimer of CFP-tagged H268Y and HA-tagged D398N mutant monomers


proof_lines: pH 7.4, 37°C, recombinant dimer of CFP-tagged A71S and HA-tagged D398N mutant monomers


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed accession P16662 via REST accessions endpoint; checked 2026-06-19. and applied mutation signature A71S to reconstruct the assayed variant sequence.

proof_lines: pH 7.4, 37°C, recombinant CFP-tagged mutant A71S dimer

proof_lines: pH 7.4, 37°C, recombinant HA-tagged mutant A71S dimer


proof_lines: pH 7.4, 37°C, recombinant CFP-tagged wild-type dimer

proof_lines: pH 7.4, 37°C, recombinant dimer of CFP-tagged wild-type and HA-tagged D398N mutant monomers

proof_lines: pH 7.4, 37°C, recombinant dimer of CFP-tagged wild-type and HA-tagged H268Y mutant monomers


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


substrate_name: Mycophenolic acid

substrate: Mycophenolic acid


substrate_name: sulfinpyrazone

substrate: sulfinpyrazone


substrate_name: retigabine

substrate: retigabine


substrate_name: naloxone

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: isoform UGT2B9, at pH 7.4, temperature not specified in the publication

substrate: naloxone


organism: Macaca mulatta

substrate_name: bilirubin

substrate: bilirubin


organism: Meleagris gallopavo


organism: Mus musculus

substrate_name: estradiol

proof_lines:
pH 7.4, 37°C
above, pH 7.4, 37°C

substrate: estradiol


organism: Oncorhynchus mykiss

proof_lines: K0.5 value, n is 2.17, liver microsome, microplate assay, animals treated with beta-naphthoflavone, pH 7, 25°C

proof_lines: K0.5 value, n is 2.21, liver microsome, pH 7, 25°C

proof_lines: K0.5 value, n is 2.57, liver microsome, microplate assay, pH 7, 25°C


organism: Oryctolagus cuniculus

substrate_name: estrone

proof_lines: liver microsomes, substrate denopamine, pH 7.5, 37°C

proof_lines: 1.5 mM morphine added, ethanol-induced enzyme

substrate: estrone


organism: Platyrrhini


substrate_name: morphine

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

substrate: morphine


proof_lines: oligomer of Ugt2b1 and Ugt1a1, expression ration Ugt1a1: Ugt2b1 is 2:2, pH not specified in the publication, temperature not specified in the publication

proof_lines: oligomer of Ugt2b1 and Ugt1a1, expression ration Ugt1a1: Ugt2b1 is 1:3, pH not specified in the publication, temperature not specified in the publication


substrate_name: androstanediol

sequence_source: UniProtKB REST direct lookup, reviewed accession Q6K1J1 (UDB31_CANLF)

substrate: androstanediol


proof_lines: recombinant UGT1A9 mutant D256N isozyme, pH.4, 37°C


sequence_source: UniProtKB REST direct lookup, reviewed accession O60656 (UD19_HUMAN)

proof_lines: recombinant wild-type isozyme UGT1A9, pH.4, 37°C


substrate_name: curcumin

proof_lines: recombinant isozyme UGT1A1, pH 7.4, 37°C

substrate: curcumin


substrate_name: phloretin

proof_lines:
isozyme UGT1A10, pH 6.4, 37°C
isozyme UGT1A9, pH 7.4, 37°C
UGT1A9/UGT2B7 heterodimer, pH 7.4, 37°C

substrate: phloretin


substrate_name: trans-androsterone

proof_lines: recombinant mutant isozyme UGT1A4 L48V, pH 8.4, 37°C

substrate: trans-androsterone


substrate_name: tigogenin

sequence_source: UniProtKB REST direct lookup, reviewed accession P22310 (UD14_HUMAN)

proof_lines: recombinant wild-type isozyme UGT1A4, pH 8.4, 37°C

substrate: tigogenin


sequence_source: UniProtKB REST direct lookup, reviewed accession P35503 (UD13_HUMAN)

proof_lines:
isozyme UGT1A3, pH 7.4, 37°C
pH 7.4, 37°C, isozyme UGT1A3
pH 7.4, 37°C


sequence_source: UniProtKB REST direct lookup, reviewed accession Q9HAW8 (UD110_HUMAN)

proof_lines: recombinant wild-type enzyme, pH 7.5, 37°C


assay_conditions_summary: cofactor CoA


substrate_name: hyodeoxycholic acid

sequence_source: UniProtKB REST direct lookup, reviewed accession P06133 (UD2B4_HUMAN)

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: hyodeoxycholic acid


organism: Rattus norvegicus

substrate_name: UDP-glucuronic acid

proof_lines: apparent Km for UGT1A6, 50mM 1-naphthol, mutants that retained activity shows similar Km values

proof_lines: apparent Km for UGT1A6, 3mM UDP-glucuronic acid, mutants that retained activity shows similar Km values

substrate: UDP-glucuronic acid


proof_lines: 17beta-hydroxysteroid UDP-glucuronosyltransferase activity, Wistar strain


organism: Serinus canaria


organism: Spheniscus humboldti


organism: Struthio camelus

substrate_name: beta-estradiol

substrate: beta-estradiol


organism: Sus scrofa


organism: Homo sapiens


substrate_name: dopamine

proof_lines: formation of dopamine-3-O-glucuronide, mutant F93L

proof_lines: formation of dopamine-4-O-glucuronide, mutant F93L

substrate: dopamine


proof_lines: formation of dopamine-3-O-glucuronide, mutant F90L

proof_lines: formation of dopamine-4-O-glucuronide, mutant F90L


substrate_name: trans-endoxifen

proof_lines: isoform UGTA10, variant 139Lys, pH 7.4, 37°C

proof_lines: isoform UGTA10, variant 139Glu, pH 7.4, 37°C

substrate: trans-endoxifen


substrate_name: lamotrigine

substrate: lamotrigine


organism: Unknown

substrate_name: (E,E)-farnesyl diphosphate

substrate: (E,E)-farnesyl diphosphate


organism: Micrococcus luteus

substrate_name: isopentenyl diphosphate

sequence_variant_note: Resolved canonical sequence from UniProtKB REST lookup for reviewed Swiss-Prot accession O82827, accessed 2026-06-19 and applied mutation signature D226A to reconstruct the assayed variant sequence.

proof_lines:
D226A mutant, pH 7.3, 37°C
mutant enzyme D226A, pH 7.3, 37°C

substrate: isopentenyl diphosphate


substrate_name: (2E,6E)-farnesyl diphosphate

sequence_variant_note: Resolved canonical sequence from UniProtKB REST lookup for reviewed Swiss-Prot accession O82827, accessed 2026-06-19 and applied mutation signature R42G to reconstruct the assayed variant sequence.

substrate: (2E,6E)-farnesyl diphosphate


sequence_variant_note: Resolved canonical sequence from UniProtKB REST lookup for reviewed Swiss-Prot accession O82827, accessed 2026-06-19 and applied mutation signature D29A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB REST lookup for reviewed Swiss-Prot accession O82827, accessed 2026-06-19 and applied mutation signature E76Q to reconstruct the assayed variant sequence.


sequence_source: UniProtKB REST lookup for reviewed Swiss-Prot accession O82827, accessed 2026-06-19

proof_lines:
wild-type, pH 7.5, 37°C
wild-type enzyme, pH 7.5, 37°C
wild type, pH 7.3, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB REST lookup for reviewed Swiss-Prot accession O82827, accessed 2026-06-19 and applied mutation signature R33A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB REST lookup for reviewed Swiss-Prot accession O82827, accessed 2026-06-19 and applied mutation signature R80A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB REST lookup for reviewed Swiss-Prot accession O82827, accessed 2026-06-19 and applied mutation signature G32R/R42G to reconstruct the assayed variant sequence.

proof_lines:
G32R/R42G mutant, pH 7.5, 37°C
G32R-R42G mutant, pH 7.5, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB REST lookup for reviewed Swiss-Prot accession O82827, accessed 2026-06-19 and applied mutation signature G32R to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB REST lookup for reviewed Swiss-Prot accession O82827, accessed 2026-06-19 and applied mutation signature E84Q to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB REST lookup for reviewed Swiss-Prot accession O82827, accessed 2026-06-19 and applied mutation signature F207S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB REST lookup for reviewed Swiss-Prot accession O82827, accessed 2026-06-19 and applied mutation signature W210A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB REST lookup for reviewed Swiss-Prot accession O82827, accessed 2026-06-19 and applied mutation signature W224A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB REST lookup for reviewed Swiss-Prot accession O82827, accessed 2026-06-19 and applied mutation signature Y148F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB REST lookup for reviewed Swiss-Prot accession O82827, accessed 2026-06-19 and applied mutation signature Y71S to reconstruct the assayed variant sequence.


substrate_name: farnesyl diphosphate

sequence_variant_note: Resolved canonical sequence from UniProtKB REST lookup for reviewed Swiss-Prot accession O82827, accessed 2026-06-19 and applied mutation signature A72L/F73L to reconstruct the assayed variant sequence.

substrate: farnesyl diphosphate


sequence_variant_note: Resolved canonical sequence from UniProtKB O82827 canonical sequence plus PMID 16420487 A72L/F73L/W78L reconstruction and applied mutation signature A72L/F73L/W78L to reconstruct the assayed variant sequence.


organism: Bacillus subtilis

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: potent competitive inhibitor against (2Z,6E,10E)-geranylgeranyl diphosphate, 37°C, pH 37°C


organism: Escherichia coli

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


proof_lines:
mutant enzyme E213A, pH 7.5, 25°C
pH 7.5, 25°C, recombinant mutant E213A
pH 7.5, 25°C, E213A mutant


proof_lines:
mutant enzymes E73A, pH 7.5, 25°C
mutant enzyme E73A, pH 7.5, 25°C


proof_lines: mutant enzymes E73A, D150A and D218A, pH 7.5, 25°C


proof_lines: mutant enzyme E73A, N74A and E81A, pH 7.5, 25°C


proof_lines:
pH 7.5, 25°C, S83(Ala)1 mutant
pH 7.5, 25°C, S83(Ala)5 mutant


substrate_name: geranylgeranyl diphosphate

proof_lines: pH 7.5, 25°C, mutant L88A, with geranylgeranyl diphosphate

substrate: geranylgeranyl diphosphate


substrate_name: geranyl diphosphate

proof_lines:
25°C, pH 7.5
alternative substrate, pH 7.5, 25°C
pH 7.5, 25°C

substrate: geranyl diphosphate


proof_lines: pH 7.5, 25°C, recombinant wild-type enzyme


proof_lines: pH 7.5, 25°C, mutant L85A, with geranylgeranyl diphosphate


proof_lines: pH 7.5, 25°C, mutant F89A, with farnesyl diphosphate


substrate_name: (2Z,6E,10E)-geranylgeranyl diphosphate

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

proof_lines: mutant enzyme N77D, pH 7.5, 37°C; mutant enzyme W78D, pH 7.5, 37°C

substrate: (2Z,6E,10E)-geranylgeranyl diphosphate


sequence_variant_note: Resolved canonical sequence from UniProtKB O82827 canonical sequence with W78I reconstructed from UniProt mutagenesis feature citing PMID 11098133 and applied mutation signature W78I to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB O82827 canonical sequence with W78R reconstructed from UniProt mutagenesis feature citing PMID 11098133 and applied mutation signature W78R to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB O82827 canonical sequence with N77D reconstructed from UniProt mutagenesis feature citing PMID 11098133 and applied mutation signature N77D to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB O82827 canonical sequence with N77A reconstructed from UniProt mutagenesis feature citing PMID 11098133 and applied mutation signature N77A to reconstruct the assayed variant sequence.


substrate_name: (Z,E,E)-Geranylgeranyl diphosphate

substrate: (Z,E,E)-Geranylgeranyl diphosphate


sequence_variant_note: Resolved canonical sequence from UniProtKB O82827 canonical sequence with F73A reconstructed from UniProt mutagenesis feature citing PMID 11346651 and applied mutation signature F73A to reconstruct the assayed variant sequence.

proof_lines:
mutant enzyme F73A, pH 7.3, 37°C
mutant enzyme F73A
F73A mutant, pH 7.3, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB O82827 canonical sequence with R197S reconstructed from UniProt mutagenesis feature citing PMID 11346651 and applied mutation signature R197S to reconstruct the assayed variant sequence.

proof_lines:
mutant enzyme R197S, pH 7.3, 37°C
R197S mutant, pH 7.3, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB O82827 canonical sequence with E201Q reconstructed from UniProt mutagenesis feature citing PMID 11346651 and applied mutation signature E201Q to reconstruct the assayed variant sequence.

proof_lines:
E201Q mutant, pH 7.3, 37°C
mutant enzyme E201Q, pH 7.3, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB O82827 canonical sequence with E216Q reconstructed from UniProt mutagenesis feature citing PMID 11346651 and applied mutation signature E216Q to reconstruct the assayed variant sequence.

proof_lines:
E216Q mutant, pH 7.3, 37°C
mutant enzyme E216Q, pH 7.3, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB O82827 canonical sequence with E193Q reconstructed from UniProt mutagenesis feature citing PMID 11346651 and applied mutation signature E193Q to reconstruct the assayed variant sequence.

proof_lines:
mutant enzyme E193Q, pH 7.3, 37°C
E193Q mutant, pH 7.3, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB O82827 canonical sequence with S74A reconstructed from UniProt mutagenesis feature citing PMID 11346651 and applied mutation signature S74A to reconstruct the assayed variant sequence.

proof_lines:
S74A mutant, pH 7.3, 37°C
mutant enzyme S74A, pH 7.3, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB O82827 canonical sequence with R203S reconstructed from UniProt mutagenesis feature citing PMID 11346651 and applied mutation signature R203S to reconstruct the assayed variant sequence.

proof_lines:
mutant enzyme R203S, pH 7.3, 37°C
R203S mutant, pH 7.3, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB O82827 canonical sequence with D221A reconstructed from UniProt mutagenesis feature citing PMID 11346651 and applied mutation signature D221A to reconstruct the assayed variant sequence.

proof_lines:
mutant enzyme D221A, pH 7.3, 37°C
D221A mutant, pH 7.3, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:P60472 via UniProt REST exact accession lookup and applied mutation signature S71A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P60472 via UniProt REST exact accession lookup and applied mutation signature E81A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P60472 via UniProt REST exact accession lookup and applied mutation signature N74A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P60472 via UniProt REST exact accession lookup and applied mutation signature R77A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P60472 via UniProt REST exact accession lookup and applied mutation signature W75A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P60472 via UniProt REST exact accession lookup and applied mutation signature E73A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P60472 via UniProt REST exact accession lookup and applied mutation signature D26K to reconstruct the assayed variant sequence.

proof_lines: pH 7.5, 25°C, D26K mutant with 0.5 mM MgCl2


sequence_source: UniProtKB:P60472 via UniProt REST exact accession lookup

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: pH 7.5, 25°C, wild type with 0.05 mM MgCl2


sequence_variant_note: Resolved canonical sequence from UniProtKB:P60472 via UniProt REST exact accession lookup and applied mutation signature D26R to reconstruct the assayed variant sequence.

proof_lines: pH 7.5, 25°C, D26R mutant with 0.5 mM MgCl2


sequence_variant_note: Resolved canonical sequence from UniProtKB:P60472 via UniProt REST exact accession lookup and applied mutation signature D26E to reconstruct the assayed variant sequence.

proof_lines: pH 7.5, 25°C, D26E mutant with 0.5 mM MgCl2


organism: Helicobacter pylori

sequence_variant_note: Resolved canonical sequence from UniProtKB:P55984 via UniProt REST exact accession lookup and applied mutation signature C234A to reconstruct the assayed variant sequence.

proof_lines: mutant C234A, pH 7.5, temperature not specified in the publication


sequence_source: UniProtKB:P55984 via UniProt REST exact accession lookup

proof_lines: wild type, pH 7.5, temperature not specified in the publication


organism: Lactiplantibacillus plantarum

sequence_source: UniProtKB:Q88VJ8 via UniProt REST exact accession lookup


sequence_variant_note: Resolved canonical sequence from UniProtKB:O82827 via UniProt REST exact accession lookup and applied mutation signature W78D to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:O82827 via UniProt REST exact accession lookup

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.


sequence_variant_note: Resolved canonical sequence from UniProtKB:O82827 via UniProt REST exact accession lookup and applied mutation signature N77Q to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P55984; reviewer residue check and mutation reconstruction; PMID:18382620 and applied mutation signature C234A to reconstruct the assayed variant sequence.

proof_lines: mutant C234A, co-substrate: farnesyl diphosphate

proof_lines: mutant C234A, co-substrate: isopentenyl diphosphate


sequence_source: UniProtKB:P55984; RCSB 2D2R/2DTN SIFTS mapping; PMID:18382620

proof_lines: wild-type, co-substrate: isopentenyl diphosphate

proof_lines: wild-type, co-substrate: farnesyl diphosphate


substrate_name: neryl diphosphate

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

substrate: neryl diphosphate


sequence_variant_note: Resolved canonical sequence from UniProtKB:O82827; reviewer residue check and mutation reconstruction; PMID:12680756 and applied mutation signature R80A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:O82827; reviewer residue check and mutation reconstruction; PMID:12680756 and applied mutation signature G32R/R42G to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:O82827; reviewer residue check and mutation reconstruction; PMID:12680756 and applied mutation signature E84Q to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:O82827; reviewer residue check and mutation reconstruction; PMID:12680756 and applied mutation signature D29A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:O82827; reviewer residue check and mutation reconstruction; PMID:12680756 and applied mutation signature R33A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:O82827; reviewer residue check and mutation reconstruction; PMID:12680756 and applied mutation signature R42G to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:O82827; reviewer residue check and mutation reconstruction; PMID:12680756 and applied mutation signature G32R to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:O82827; reviewer residue check and mutation reconstruction; PMID:12680756 and applied mutation signature E76Q to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:O82827; reviewer residue check and mutation reconstruction; PMID:14769870 and applied mutation signature Y71S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed accession O82827; W210A reconstructed from canonical O82827 after direct residue check. and applied mutation signature W210A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed accession O82827; Y148F reconstructed from canonical O82827 after direct residue check. and applied mutation signature Y148F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB reviewed accession O82827; F207S reconstructed from canonical O82827 after direct residue check. and applied mutation signature F207S to reconstruct the assayed variant sequence.


sequence_source: UniProtKB reviewed accession O82827 from direct EC+organism lookup.

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: pH 7.5, 30°C, recombinant wild-type enzyme


proof_lines: substrate (Z,E,E)-geranylgeranyl diphosphate, pH 7.5, 37°C


organism: Saccharomyces cerevisiae


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


proof_lines:
pH 7.5, 25°C, recombinant mutant H43A
pH 7.5, 25°C, H43A mutant


proof_lines:
pH 7.5, 25°C, recombinant mutant H199A
pH 7.5, 25°C, H199A mutant


proof_lines: pH 7.5, 25°C, recombinant mutant H43A and mutant E213A


proof_lines:
pH 7.5, 25°C, recombinant mutant D26A
mutant enzyme D26A, pH 7.5, 25°C
pH 7.5, 25°C, mutant D26A


proof_lines: pH 7.5, 25°C, recombinant mutant H199A/E213A


enzyme_display_name: carbonic anhydrase

assay_conditions_summary: pH 6.3; 298.15 K; buffer carbonic anhydrase II

proof_lines: pH 8.7, 1,2-dimethylimidazole buffer, carbonic anhydrase II

enzyme: carbonic anhydrase


organism: Methanosarcina thermophila

assay_conditions_summary: pH 7.5; 298.15 K; buffer MOPS/MOPS-Ac/IMID

proof_lines: pH 7.5, 25°C, variant E84A, 50 mM MOPS-Ac buffer

proof_lines: pH 7.5, 25°C, variant E84A, 50 mM MOPS buffer

proof_lines: pH 7.5, 25°C, variant E84A, 50 mM IMID buffer


organism: Methanothermobacter thermautotrophicus

assay_conditions_summary: pH 8.5; 298.15 K; buffer MOPS or imidazole

proof_lines: pH 8.5, 25°C, mutant D34A, imidazole buffer


assay_conditions_summary: pH 8.5; 298.15 K; buffer imidazole or MOPS

proof_lines: pH 8.5, 25°C, mutant D34A/R36A, imidazole buffer

proof_lines: pH 8.5, 25°C, mutant D34A/R36A, MOPS buffer


proof_lines: pH 8.5, 25°C, mutant R36A, imidazole buffer


sequence_source: UniProt P40881 FASTA with mature-position reconstruction using UniProt signal peptide 1-34

sequence_variant_note: Mutation signature E62A could not be applied safely because the resolved sequence does not match the expected reference residue(s).

assay_conditions_summary: pH 7.5; 298.15 K; buffer notes

proof_lines: pH 7.5, 25°C, variant E62A, 50 mM IMID buffer

proof_lines: pH 7.5, 25°C, variant E62A, 50 mM MOPS-Ac buffer

proof_lines: pH 7.5, 25°C, variant E62A, 50 mM MOPS buffer


enzyme_display_name: dihydrofolate reductase

organism: Mycobacterium avium

sequence_source: UniProt REST O30463

sequence_variant_note: Resolved canonical sequence from UniProt REST O30463 and applied mutation signature L32F to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 7; 303.15 K; cofactor NADPH

enzyme: dihydrofolate reductase


substrate_name: NADPH

sequence_variant_note: Resolved canonical sequence from UniProt REST O30463 and applied mutation signature D31A to reconstruct the assayed variant sequence.

substrate: NADPH


organism: Mycobacterium tuberculosis

substrate_name: Unknown

sequence_source: UniProt REST P9WNX1

substrate: Unknown


organism: Photobacterium profundum

substrate_name: dihydrofolate

sequence_source: UniProt REST D1MX70

assay_conditions_summary: pH 7; 298.15 K; cofactor NADPH

proof_lines: pH 7.0, 25°C, under atmospheric pressure

substrate: dihydrofolate


organism: Plasmodium falciparum

sequence_source: UniProt REST P13922

sequence_variant_note: Resolved canonical sequence from UniProt REST P13922 and applied mutation signature Y35G/F37L to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 5; 298.15 K; cofactor NADPH


sequence_variant_note: Resolved canonical sequence from UniProt REST P13922 and applied mutation signature Y35L/F37T to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST P13922 and applied mutation signature Y35L/F37R to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST P13922 and applied mutation signature K27E to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST P13922 and applied mutation signature Y35Q/F37R to reconstruct the assayed variant sequence.


organism: Plasmodium knowlesi

sequence_source: UniProt REST A0A384KN43

assay_conditions_summary: pH 6; cofactor NADPH

proof_lines: pH 6, temperature not specified in the publication, isolate PKNY146CSP


organism: Plasmodium vivax

sequence_source: UniProt REST O02604

sequence_variant_note: Resolved canonical sequence from UniProt REST O02604 and applied mutation signature S58R/S117N to reconstruct the assayed variant sequence.


organism: Pneumocystis carinii

sequence_source: UniProt REST P16184

sequence_variant_note: Resolved canonical sequence from UniProt REST P16184 and applied mutation signature F69N to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 7.4; 310.15 K; cofactor NADPH


sequence_variant_note: Resolved canonical sequence from UniProt REST P16184 and applied mutation signature K37Q/F69N to reconstruct the assayed variant sequence.

proof_lines: recombinant mutant K37Q/F69N, pH 7.4, 37°C


sequence_variant_note: Resolved canonical sequence from UniProt REST P16184 and applied mutation signature K37S/F69N to reconstruct the assayed variant sequence.

proof_lines: recombinant mutant K37S/F69N, pH 7.4, 37°C


organism: Homo sapiens

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

proof_lines: recombinant mutant Q35S/N64S, pH 7.4, 37°C


proof_lines: recombinant mutant N64F enzyme, pH 7.4, 37°C


organism: Leishmania braziliensis


organism: Methylorubrum extorquens


organism: Moritella japonica


organism: Moritella yayanosii


organism: Schistosoma mansoni

sequence_source: UniProtKB REST exact accession lookup on 2026-06-19 (current accession G4VJD6).

assay_conditions_summary: pH 7.8; 298.15 K; cofactor NADP+


organism: Shewanella violacea

sequence_source: UniProtKB REST exact accession lookup on 2026-06-19 (current accession D1MYR1).


organism: Staphylococcus aureus

sequence_source: UniProtKB REST exact accession lookup on 2026-06-19 (current accession P0A017).

assay_conditions_summary: cofactor NADPH


sequence_source: UniProtKB REST exact accession lookup on 2026-06-19 provided canonical P0A017; row-specific variant sequence not reconstructed.


sequence_source: UniProtKB REST exact accession lookup on 2026-06-19 (current accession A0A0M3KKX1).

proof_lines: 30°C, pH not specified in the publication


organism: Streptococcus pneumoniae

sequence_source: UniProtKB REST exact accession lookup on 2026-06-19 provided canonical Q54801; row-specific variant sequence not reconstructed.

proof_lines: mutant H26Y/Q60K/A77V/V78A/Q81H/Q91S/L100V/E133A/A149T, pH 7.0, 25°C, value below

proof_lines: mutant H26Y/Q60K/A77V/V78A/Q81H/Q91S/L100V/E133A/A149T, pH 7.0, 25°C, value above


sequence_source: UniProtKB REST exact accession lookup on 2026-06-19 (current accession Q54801).


organism: Thermotoga maritima

sequence_source: UniProtKB REST exact accession lookup on 2026-06-19 provided canonical Q60034; row-specific variant sequence not reconstructed.


sequence_source: UniProtKB REST exact accession lookup on 2026-06-19 (current accession Q60034).


organism: Trypanosoma brucei

sequence_source: UniProtKB REST exact accession lookup on 2026-06-19 (current accession Q27783).


organism: Trypanosoma cruzi

sequence_source: UniProtKB REST exact accession lookup on 2026-06-19 (current accession Q27793).


sequence_variant_note: Resolved canonical sequence from UniProtKB:P16184 direct REST lookup on 2026-06-19; Canonical UniProtKB:P16184 residue F69 matched the selected F69S signature; substituted S at position 69 with no numbering offset. and applied mutation signature F69S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P16184 direct REST lookup on 2026-06-19; Canonical UniProtKB:P16184 residue K37 matched the selected K37S signature; substituted S at position 37 with no numbering offset. and applied mutation signature K37S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P16184 direct REST lookup on 2026-06-19; Canonical UniProtKB:P16184 residue K37 matched the selected K37Q signature; substituted Q at position 37 with no numbering offset. and applied mutation signature K37Q to reconstruct the assayed variant sequence.

proof_lines:
recombinant mutant K37Q, pH 7.4, 37°C
mutant K37Q enzyme, pH 7.4, 37°C


sequence_source: UniProtKB:P16184 direct REST lookup on 2026-06-19; Canonical UniProtKB:P16184 sequence used for the wild-type row.

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: recombinant wild-type enzyme, pH 7.4, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:P16184 direct REST lookup on 2026-06-19; Canonical UniProtKB:P16184 residues K37 and F69 matched the selected K37S/F69S signature; substituted S at positions 37 and 69 with no numbering offset. and applied mutation signature K37S/F69S to reconstruct the assayed variant sequence.

proof_lines: recombinant mutant K37S/F69S, pH 7.4, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:P16184 direct REST lookup on 2026-06-19; Canonical UniProtKB:P16184 residues K37 and F69 matched the selected K37Q/F69S signature; substituted Q at position 37 and S at position 69 with no numbering offset. and applied mutation signature K37Q/F69S to reconstruct the assayed variant sequence.

proof_lines: recombinant mutant K37Q/F69S, pH 7.4, 37°C


organism: Pneumocystis jirovecii


sequence_source: UniProtKB:Q9UUP5 direct REST lookup on 2026-06-19; Canonical UniProtKB:Q9UUP5 sequence used for the wild-type row.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9UUP5 direct REST lookup on 2026-06-19; Canonical UniProtKB:Q9UUP5 residue S69 matched the selected S69N signature; substituted N at position 69 with no numbering offset. and applied mutation signature S69N to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9UUP5 direct REST lookup on 2026-06-19; Canonical UniProtKB:Q9UUP5 residue S37 matched the selected S37Q signature; substituted Q at position 37 with no numbering offset. and applied mutation signature S37Q to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9UUP5 direct REST lookup on 2026-06-19; Canonical UniProtKB:Q9UUP5 residues S37 and S69 matched the selected S37Q/S69N signature; substituted Q at position 37 and N at position 69 with no numbering offset. and applied mutation signature S37Q/S69N to reconstruct the assayed variant sequence.

proof_lines: recombinant mutant S37Q/S69N, pH 7.4, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9UUP5 direct REST lookup on 2026-06-19; Canonical UniProtKB:Q9UUP5 residue S69 matched the selected S69F signature; substituted F at position 69 with no numbering offset. and applied mutation signature S69F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9UUP5 direct REST lookup on 2026-06-19; Canonical UniProtKB:Q9UUP5 residues S37 and S69 matched the selected S37Q/S69F signature; substituted Q at position 37 and F at position 69 with no numbering offset. and applied mutation signature S37Q/S69F to reconstruct the assayed variant sequence.

proof_lines: recombinant mutant S37Q/S69F, pH 7.4, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9UUP5 direct REST lookup on 2026-06-19; Canonical UniProtKB:Q9UUP5 residues S37 and S69 matched the selected S37K/S69F signature; substituted K at position 37 and F at position 69 with no numbering offset. and applied mutation signature S37K/S69F to reconstruct the assayed variant sequence.

proof_lines: recombinant mutant S37K/S69F, pH 7.4, 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9UUP5 direct REST lookup on 2026-06-19; Canonical UniProtKB:Q9UUP5 residues S37 and S69 matched the selected S37K/S69N signature; substituted K at position 37 and N at position 69 with no numbering offset. and applied mutation signature S37K/S69N to reconstruct the assayed variant sequence.

proof_lines: recombinant mutant S37K/S69N, pH 7.4, 37°C


organism: Bacteria

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: bacterial chromosomal encoded isozyme type III

proof_lines: bacterial, R factor encoded isozymes type I

proof_lines: bacterial, R factor encoded isozyme type II


organism: Lacticaseibacillus casei

assay_conditions_summary: pH 6.5; cofactor NADPH

proof_lines:
isozyme I
+ NADPH
pH 6.5


organism: Salmonella enterica subsp. enterica serovar Typhimurium


organism: Brugia malayi

proof_lines: above, pH 6.0, 25°C recombinant His-tagged enzyme


organism: Burkholderia cepacia

proof_lines:
strain PC 174
strain PC 178


organism: Candida albicans


organism: Cricetulus griseus


organism: Crithidia fasciculata

proof_lines: with 7,8-dihydrofolate or 6-hydroxymetylpterin


organism: Enterococcus faecalis


organism: Enterococcus faecium


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


organism: Escherichia coli


organism: Babesia gibsoni

sequence_source: UniProt REST accession B5U9U8

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

assay_conditions_summary: 298.15 K; cofactor NADPH

proof_lines:
25°C, recombinant GST-tagged enzyme
25°C, recombinant detagged enzyme


organism: Bacillus anthracis

sequence_source: UniProt REST accession Q81R22

sequence_variant_note: Resolved canonical sequence from UniProt REST accession Q81R22 and applied mutation signature F96I to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST accession Q81R22 and applied mutation signature Y102F to reconstruct the assayed variant sequence.


organism: Bombyx mori

sequence_source: UniProt REST accession J7GQW2


organism: Drosophila melanogaster

sequence_source: UniProt REST accession P17719

sequence_variant_note: Resolved canonical sequence from UniProt REST accession P17719 and applied mutation signature L30Q to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 8.5; 298.15 K; cofactor NADPH


sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.


sequence_source: UniProt REST accession Q834R2

proof_lines: pH and temperature not specified in the publication


sequence_source: UniProt REST accession P0ABQ4

sequence_variant_note: Resolved canonical sequence from UniProt REST accession P0ABQ4 and applied mutation signature M1A/M16N/M20L/M42Y/M92F/C85A/C152S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST accession P0ABQ4 and applied mutation signature M1A/C85A/C152S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST accession P0ABQ4 and applied mutation signature M1A/M16F/M20L/M42Y/M92F/C85A/C152S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST accession P0ABQ4 and applied mutation signature M1S/C85A/C152S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST accession P0ABQ4 and applied mutation signature M1P/C85A/C152S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST accession P0ABQ4 and applied mutation signature M1A/M16S/M20L/M42Y/M92F/C85A/C152S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST accession P0ABQ4 and applied mutation signature C85A/C152S to reconstruct the assayed variant sequence.


organism: Homo sapiens

sequence_variant_note: Resolved canonical sequence from UniProt REST direct check: P00374 / DYR_HUMAN, Homo sapiens dihydrofolate reductase, EC 1.5.1.3, canonical sequence length 187 and applied mutation signature F31R/Q35E with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST direct check: P00374 / DYR_HUMAN, Homo sapiens dihydrofolate reductase, EC 1.5.1.3, canonical sequence length 187 and applied mutation signature F31R/F34A/Q35N with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST direct check: P00374 / DYR_HUMAN, Homo sapiens dihydrofolate reductase, EC 1.5.1.3, canonical sequence length 187 and applied mutation signature F31P/Q35H with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST direct check: P00374 / DYR_HUMAN, Homo sapiens dihydrofolate reductase, EC 1.5.1.3, canonical sequence length 187 and applied mutation signature F31A/F34V/Q35H with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST direct check: P00374 / DYR_HUMAN, Homo sapiens dihydrofolate reductase, EC 1.5.1.3, canonical sequence length 187 and applied mutation signature F31P with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST direct check: P00374 / DYR_HUMAN, Homo sapiens dihydrofolate reductase, EC 1.5.1.3, canonical sequence length 187 and applied mutation signature F31S/Q35E with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST direct check: P00374 / DYR_HUMAN, Homo sapiens dihydrofolate reductase, EC 1.5.1.3, canonical sequence length 187 and applied mutation signature F31R/F34T/Q35R with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST direct check: P00374 / DYR_HUMAN, Homo sapiens dihydrofolate reductase, EC 1.5.1.3, canonical sequence length 187 and applied mutation signature F31G/Q35N with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_source: UniProt REST direct check: P00374 / DYR_HUMAN, Homo sapiens dihydrofolate reductase, EC 1.5.1.3, canonical sequence length 187

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: pH 7.4, 37°C, wild-type enzyme and mutant N64F


assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

proof_lines: pH 7.5, 25°C, cosubstrate: dihydrofolate


sequence_variant_note: Resolved canonical sequence from UniProt REST direct check: P00374 / DYR_HUMAN, Homo sapiens dihydrofolate reductase, EC 1.5.1.3, canonical sequence length 187 and applied mutation signature N64S with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.

proof_lines:
recombinant mutant N64S, pH 7.4, 37°C
pH 7.4, 37°C, mutant N64S


sequence_variant_note: Resolved canonical sequence from UniProt REST direct check: P00374 / DYR_HUMAN, Homo sapiens dihydrofolate reductase, EC 1.5.1.3, canonical sequence length 187 and applied mutation signature Q35S with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.

proof_lines:
recombinant mutant Q35S, pH 7.4, 37°C
pH 7.4, 37°C, mutant Q35S


sequence_variant_note: Resolved canonical sequence from UniProt REST direct check: P00374 / DYR_HUMAN, Homo sapiens dihydrofolate reductase, EC 1.5.1.3, canonical sequence length 187 and applied mutation signature Q35K with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.

proof_lines:
recombinant mutant Q35K, pH 7.4, 37°C
pH 7.4, 37°C, mutant Q35K


sequence_variant_note: Resolved canonical sequence from UniProt REST direct check: P00374 / DYR_HUMAN, Homo sapiens dihydrofolate reductase, EC 1.5.1.3, canonical sequence length 187 and applied mutation signature Q35K/N64F with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.

proof_lines: recombinant mutant Q35K/N64F, pH 7.4, 37°C


sequence_variant_note: Resolved canonical sequence from UniProt REST direct check: P00374 / DYR_HUMAN, Homo sapiens dihydrofolate reductase, EC 1.5.1.3, canonical sequence length 187 and applied mutation signature Q35S/N64F with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.

proof_lines: recombinant mutant Q35S/N64F, pH 7.4, 37°C


sequence_variant_note: Resolved canonical sequence from UniProt REST direct check: P00374 / DYR_HUMAN, Homo sapiens dihydrofolate reductase, EC 1.5.1.3, canonical sequence length 187 and applied mutation signature Q35K/N64S with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.

proof_lines: recombinant mutant Q35K/N64S, pH 7.4, 37°C


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: mutant enzyme R44F, at 15°C, pH not specified in the publication


proof_lines: mutant enzyme R44N, at 15°C, pH not specified in the publication


proof_lines: mutant enzyme R44L, at 15°C, pH not specified in the publication


proof_lines: mutant enzyme R44M, at 15°C, pH not specified in the publication


proof_lines: mutant enzyme R44G, at 15°C, pH not specified in the publication


proof_lines: mutant enzyme R44D, at 15°C, pH not specified in the publication


proof_lines: mutant enzyme R44S, at 15°C, pH not specified in the publication


proof_lines: mutant enzyme R44I, at 15°C, pH not specified in the publication


assay_conditions_summary: pH 7; 293.15 K; cofactor NADPH

proof_lines: pH 7, 20°C, dimeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface

proof_lines: pH 7, 20°C, monomeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface


sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: wild type enzyme, at 15°C, pH not specified in the publication


proof_lines:
with NADPH
with acetylpyridine adenine nucleotide
with 7,8-dihydrofolate


proof_lines: triple mutant L16M/L20M/L42M from plasmid


organism: Halalkalibacterium halodurans

assay_conditions_summary: pH 7.5; 310.15 K; cofactor NADPH

proof_lines: His-tagged recombinant enzyme, pH 7.5, 37°C


organism: Haloarcula japonica

assay_conditions_summary: pH 6; 298.15 K; cofactor NADPH

proof_lines:
pH 6.0, 25°C
pH 8.0, 25°C, presence of 1000 mM NaCl
pH 8.0, 25°C, presence of 500 mM NaCl


organism: Haloferax volcanii


proof_lines: mutant carrying the K32M mutation in gene copies 1 and 3 with the Q67H mutation in all gene copies, pH 7.0, 30°C

proof_lines: mutant isoform that carries the K32M mutation in gene copies 1 and 3 and the Q67H mutation in gene copies 2 and 4, pH 7.0, 30°C


proof_lines: mutant enzyme R44V, at 15°C, pH not specified in the publication


proof_lines: mutant enzyme R44K, at 15°C, pH not specified in the publication


proof_lines: mutant enzyme R44E, at 15°C, pH not specified in the publication


proof_lines: mutant enzyme R44A, at 15°C, pH not specified in the publication


proof_lines: mutant enzyme R44Q, at 15°C, pH not specified in the publication


proof_lines: mutant enzyme R44W, at 15°C, pH not specified in the publication


proof_lines: mutant enzyme R44T, at 15°C, pH not specified in the publication


proof_lines: mutant enzyme R44C, at 15°C, pH not specified in the publication


proof_lines: mutant enzyme R44H, at 15°C, pH not specified in the publication


proof_lines: mutant enzyme R44Y, at 15°C, pH not specified in the publication


proof_lines: mutant enzyme R44P, at 15°C, pH not specified in the publication


organism: Leishmania major

substrate_name: folate

sequence_source: UniProtKB:P07382 reviewed EC+organism lookup; sequence fetched from UniProtKB REST on 2026-06-19

proof_lines: bifunctional enzyme DHFR-thymidylate synthase

substrate: folate


organism: Leishmania tropica


organism: Mus musculus

proof_lines:
altered enzyme
pH 7.4, 37°C


proof_lines:
wild-type enzyme
wild-type


organism: Mycobacterium tuberculosis

proof_lines: with NADH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5

proof_lines: with NAPH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5

proof_lines: Km less than 0.001 mM, at 25°C and 100 mM HEPES, pH 7.5


organism: Plasmodium berghei

proof_lines:
trimethoprim-sensitive strain
trimethoprim-resistant strain
trimethoprim-resistant sdtrain


organism: Plasmodium falciparum


proof_lines:
wild-type, 25°C, pH 7.0
recombinant wild-type enzyme
wild-type, pH 8.0, 25°C


sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.


proof_lines:
mutant linear ANLYF-G6, 15°C, pH 7.0
mutant circular ANLYF-G6, 15°C, pH 7.0
mutant circular ANLYF-G8, 15°C, pH 7.0


mutation_signature: deletion mutant DELTAAla145

proof_lines: deletion mutant DELTAAla145, at pH 7.0 and 25°C


mutation_signature: deletion mutant DELTAArg52/DELTAGly67

proof_lines: deletion mutant DELTAArg52/DELTAGly67, at pH 7.0 and 25°C


mutation_signature: deletion mutant DELTAArg52/DELTAGly121

proof_lines: deletion mutant DELTAArg52/DELTAGly121, at pH 7.0 and 25°C


mutation_signature: deletion mutant DELTAArg52

proof_lines: deletion mutant DELTAArg52, at pH 7.0 and 25°C


mutation_signature: deletion mutant DELTAGly121/DELTAAla145

proof_lines: deletion mutant DELTAGly121/DELTAAla145, at pH 7.0 and 25°C


mutation_signature: deletion mutant DELTAGly67/DELTAAla145

proof_lines: deletion mutant DELTAGly67/DELTAAla145, at pH 7.0 and 25°C


mutation_signature: deletion mutant DELTAGly67/DELTAGly121

proof_lines: deletion mutant DELTAGly67/DELTAGly121, at pH 7.0 and 25°C


mutation_signature: deletion mutant DELTAGly67

proof_lines: deletion mutant DELTAGly67, at pH 7.0 and 25°C


mutation_signature: deletion mutant DELTAArg52/DELTAAla145

proof_lines: deletion mutant DELTAArg52/DELTAAla145, at pH 7.0 and 25°C


mutation_signature: deletion mutant DELTAGly121

proof_lines: deletion mutant DELTAGly121, at pH 7.0 and 25°C


sequence_source: UniProtKB:P0ABQ4 direct REST lookup

proof_lines:
wild-type, pH 7, 25°C
wild-type, 15°C, pH 7.0
wild type enzyme, at pH 7.0 and 25°C


proof_lines: chimera with Listeria grayi enzyme, pH 7, 25°C

proof_lines: chimera with Haemophilus influenzae enzyme, pH 7, 25°C

proof_lines: chimera with Streptococcus dysgalactiae enzyme, pH 7, 25°C


proof_lines: recombinant mutants S108G, S108N and S108C


proof_lines: recombinant mutants S108G, S108A and S108C


organism: Plasmodium vivax


organism: Pneumocystis carinii


assay_conditions_summary: pH 7; cofactor NADPH

proof_lines: recombinant enzyme, pH 7.0, 0.1 M imidazole


organism: Pneumocystis jirovecii

assay_conditions_summary: pH 7.65; cofactor NADPH

proof_lines: His-tagged human-derived enzyme, pH 7.65


proof_lines: lowest Km value in 3.0 M KCl, recombinant wild-type enzyme


organism: Heliothis virescens


assay_conditions_summary: pH 6; 310.15 K; cofactor NADPH

proof_lines:
recombinant enzyme
pH 6.0
pH 7.4, 37°C


proof_lines:
wild-type, pH 7.6, 23°C
wild-type, pH 7.0, 25°C


organism: Human gammaherpesvirus 8


organism: Escherichia coli

sequence_variant_note: Resolved canonical sequence from reconstructed_variant_from_uniprot:P0ABQ4 and applied mutation signature M42I to reconstruct the assayed variant sequence.

data_origin: experimental


sequence_variant_note: Resolved canonical sequence from reconstructed_variant_from_uniprot:P0ABQ4 and applied mutation signature M42Q to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed_variant_from_uniprot:P0ABQ4 and applied mutation signature M42T to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed_variant_from_uniprot:P0ABQ4 and applied mutation signature M42V to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed_variant_from_uniprot:P0ABQ4 and applied mutation signature M42A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed_variant_from_uniprot:P0ABQ4 and applied mutation signature M42H to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed_variant_from_uniprot:P0ABQ4 and applied mutation signature M42W to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed_variant_from_uniprot:P0ABQ4 and applied mutation signature M42E to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed_variant_from_uniprot:P0ABQ4 and applied mutation signature M42S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed_variant_from_uniprot:P0ABQ4 and applied mutation signature M42C to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed_variant_from_uniprot:P0ABQ4 and applied mutation signature M42Y to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed_variant_from_uniprot:P0ABQ4 and applied mutation signature M42G to reconstruct the assayed variant sequence.


organism: Saccharomyces cerevisiae

proof_lines:
with NADPH
with 7,8-dihydrofolate
with N10-formyldihydrofolate


organism: Staphylococcus aureus


organism: Stenotrophomonas maltophilia

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADPH


organism: Sus scrofa


organism: Tequatrovirus T4


organism: Thermotoga maritima


organism: Trypanosoma brucei

assay_conditions_summary: pH 7; 310.15 K; cofactor NADPH

proof_lines: wild type enzyme, in 50 mM TES, pH 7.0, at 37°C


proof_lines: mutant enzyme T86N, in 50 mM TES, pH 7.0, at 37°C


proof_lines: mutant enzyme T86S, in 50 mM TES, pH 7.0, at 37°C


assay_conditions_summary: pH 7; 298.15 K; cofactor NADPH


assay_conditions_summary: pH 8; 298.15 K; cofactor NADPH


enzyme_display_name: carboxylesterase

organism: Homo sapiens

substrate_name: tributyrin

assay_conditions_summary: pH 7.5; 298.15 K; buffer 0.1 M potassium phosphate

proof_lines:
pH 7.5, 25°C
pH 7.0, 37°C, isoform CES2
pH 7.0, 37°C

enzyme: carboxylesterase

substrate: tributyrin


organism: Rattus norvegicus

proof_lines:
hydrolase A, pH 7.4, 37°C
hydrolase B, pH 7.4, 37°C
pH 5.0, 37°C


organism: Reticulitermes flavipes

substrate_name: Alpha-naphthyl acetate

proof_lines:
isozyme ME3, pH 7.0, 27°C
isozyme ME2, pH 7.0, 27°C
isozyme ME1, pH 7.0, 27°C

substrate: Alpha-naphthyl acetate


proof_lines:
pH 7.4, 37°C
at pH 7.4 and 37Â°C
isoenzyme V


organism: Actinidia eriantha

substrate_name: 4-methylumbelliferyl heptanoate

sequence_source: UniProtKB reviewed accession Q0ZPV7 direct lookup

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

substrate: 4-methylumbelliferyl heptanoate


organism: Aeropyrum pernix

substrate_name: 4-nitrophenyl octanoate

substrate: 4-nitrophenyl octanoate


substrate_name: 4-nitrophenyl dodecanoate

proof_lines: pH 8.0, 60°C, mutant enzyme W474Q/R526V/T560W

substrate: 4-nitrophenyl dodecanoate


proof_lines:
pH 8.0, 60°C, wild-type enzyme
pH 7.0, 70°C, wild-type enzyme


proof_lines: pH 8.0, 60°C, mutant enzyme F488S/R526V/T560W


proof_lines: pH 8.0, 60°C, mutant enzyme F488A/R526V/T560W


proof_lines: pH 8.0, 60°C, mutant enzyme F488W/R526V/T560W


proof_lines: pH 8.0, 60°C, mutant enzyme F488P/R526V/T560W


proof_lines: pH 8.0, 60°C, mutant enzyme F488G/R526V/T560W


proof_lines: pH 8.0, 60°C, mutant enzyme W474V/F488G/R526V/T560W


proof_lines: pH 8.0, 60°C, mutant enzyme W474A/R526V/T560W


proof_lines: pH 8.0, 60°C, mutant enzyme W474V/R526V/T560W


proof_lines: pH 8.0, 60°C, mutant enzyme F488Y/R526V/T560W


organism: Alicyclobacillus tolerans

substrate_name: 2-naphthyl acetate

substrate: 2-naphthyl acetate


organism: Altericroceibacterium indicum

sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A845ADQ8 direct lookup plus selected mutation signature reconstruction and applied mutation signature T375I to reconstruct the assayed variant sequence.

proof_lines:
mutant enzyme T375I, at pH 7.4 and 37°C
mutant enzyme T375I, at pH 7.5 and 30°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A845ADQ8 direct lookup plus selected mutation signature reconstruction and applied mutation signature E428A to reconstruct the assayed variant sequence.

proof_lines:
mutant enzyme E428A, at pH 7.4 and 37°C
mutant enzyme E428A, at pH 7.5 and 30°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A845ADQ8 direct lookup plus selected mutation signature reconstruction and applied mutation signature Y318A to reconstruct the assayed variant sequence.

proof_lines:
mutant enzyme Y318A, at pH 7.4 and 37°C
mutant enzyme Y318A, at pH 7.5 and 30°C


substrate_name: 4-nitrophenyl hexanoate

sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A845ADQ8 direct lookup plus selected mutation signature reconstruction and applied mutation signature Y318F to reconstruct the assayed variant sequence.

proof_lines:
mutant enzyme Y318F, at pH 7.5 and 30°C
mutant enzyme Y318F, at pH 7.4 and 37°C

substrate: 4-nitrophenyl hexanoate


sequence_source: UniProtKB:A0A845ADQ8 direct lookup

proof_lines:
wild type enzyme, at pH 7.4 and 37°C
wild type enzyme, at pH 7.5 and 30°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A845ADQ8 direct lookup plus selected mutation signature reconstruction and applied mutation signature Q193A to reconstruct the assayed variant sequence.

proof_lines:
mutant enzyme Q193A, at pH 7.4 and 37°C
mutant enzyme Q193A, at pH 7.5 and 30°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A845ADQ8 direct lookup plus selected mutation signature reconstruction and applied mutation signature T375A to reconstruct the assayed variant sequence.

proof_lines:
mutant enzyme T375A, at pH 7.4 and 37°C
mutant enzyme T375A, at pH 7.5 and 30°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A845ADQ8 direct lookup plus selected mutation signature reconstruction and applied mutation signature W107A to reconstruct the assayed variant sequence.

proof_lines:
mutant enzyme W107A, at pH 7.4 and 37°C
mutant enzyme W107A, at pH 7.5 and 30°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A845ADQ8 direct lookup plus selected mutation signature reconstruction and applied mutation signature M273A to reconstruct the assayed variant sequence.

proof_lines:
mutant enzyme M273A, at pH 7.5 and 30°C
mutant enzyme M273A, at pH 7.4 and 37°C


organism: Aquamicrobium sp. FPB-1

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: recombinant His-tagged enzyme, pH 7.0, 37°C


organism: Archaeoglobus fulgidus

substrate_name: 4-nitrophenyl propionate

sequence_source: UniProtKB:O28558 direct lookup

substrate: 4-nitrophenyl propionate


substrate_name: 4-nitrophenyl palmitate

sequence_source: UniProtKB:O28511 direct lookup

substrate: 4-nitrophenyl palmitate


organism: Bacillus sp. SCSIO 15121

substrate_name: 4-nitrophenyl butyrate

substrate: 4-nitrophenyl butyrate


organism: Bacillus subtilis

substrate_name: 4-nitrophenyl butanoate

sequence_source: UniProtKB:Q4PNS3 direct lookup

substrate: 4-nitrophenyl butanoate


substrate_name: p-nitrophenyl acetate

sequence_source: UniProtKB:P37967 direct lookup

substrate: p-nitrophenyl acetate


substrate_name: retinyl palmitate

sequence_source: UniProtKB release 2026_02 accession P16303

substrate: retinyl palmitate


sequence_source: UniProtKB release 2026_02 accession G3V7J5; O35535 is merged to G3V7J5


organism: Saccharolobus islandicus

sequence_source: UniProtKB release 2026_02 accession F0NDQ1

assay_conditions_summary: pH 8

proof_lines: pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Sulfolobus solfataricus

proof_lines: pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I)

proof_lines: pH 8.0, temperature not specified in the publication, recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II)


organism: Saccharolobus shibatae

sequence_source: UniProtKB release 2026_02 accession Q5NU42


sequence_variant_note: Resolved canonical sequence from Variant reconstructed from UniProtKB release 2026_02 accession Q5NU42 and applied mutation signature A241N/Q249P/G250E/F276S to reconstruct the assayed variant sequence.

proof_lines: pH 6.0, 35°C, mutant enzyme A241N/Q249P/G250E/F276S


sequence_variant_note: Resolved canonical sequence from Variant reconstructed from UniProtKB release 2026_02 accession Q5NU42 and applied mutation signature Q249P/G250E to reconstruct the assayed variant sequence.


organism: Saccharolobus solfataricus

substrate_name: 4-nitrophenyl hexadecanoate

substrate: 4-nitrophenyl hexadecanoate


sequence_source: Canonical context from UniProtKB release 2026_02 accession Q97VU2; row-specific deletion not resolved

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

proof_lines: pH 7.1, 70°C, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus


substrate_name: 4-nitrophenyl caproate

sequence_source: UniProtKB release 2026_02 accession Q97VU1

proof_lines: pH and temperature not specified in the publication

substrate: 4-nitrophenyl caproate


substrate_name: 4-nitrophenyl caprylate

verification_status: corrected

sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q97VU1 and applied mutation signature R210A to reconstruct the assayed variant sequence.

column_label: Table 1 on journal page 1154, p-Nitrophenyl caprylate block, enzyme row R210A; column kcat/Km (s^-1 mM^-1) = 192; column Km (uM) = 43; column kcat (s^-1) = 7.8.

normalized_value_unit_evidence: kcat: printed Table 1 cell 7.8 s^-1; the printed column is already per second, so no unit conversion applies and the value carries over as 7.8 s^(-1). Km: printed Table 1 cell 43 uM; 1 uM is 1/1000 mM, so 43 / 1000 = 0.043 mM. kcat/Km: printed Table 1 cell 192 s^-1 mM^-1; the printed unit is the canonical s^(-1)*mM^(-1), so no unit conversion applies; this catalog row carries no efficiency field, so the cell is cited through kcat_over_km_provenance only.

raw_value_unit_evidence: Table 1, enzyme row R210A, p-Nitrophenyl caprylate block: kcat/Km = 192 s^-1 mM^-1; Km = 43 uM; kcat = 7.8 s^-1.

table_label: Table 1: Kinetic parameters of wild-type SsoPEst and R210A (Biotechnology Letters 2010, volume 32, pages 1151-1157)

substrate: 4-nitrophenyl caprylate

status: corrected


column_label: Table 1 on journal page 1154, p-Nitrophenyl caprylate block, enzyme row Wild-type; column kcat/Km (s^-1 mM^-1) = 63; column Km (uM) = 28; column kcat (s^-1) = 1.7.

normalized_value_unit_evidence: kcat: printed Table 1 cell 1.7 s^-1; the printed column is already per second, so no unit conversion applies and the value carries over as 1.7 s^(-1). Km: printed Table 1 cell 28 uM; 1 uM is 1/1000 mM, so 28 / 1000 = 0.028 mM. kcat/Km: printed Table 1 cell 63 s^-1 mM^-1; the printed unit is the canonical s^(-1)*mM^(-1), so no unit conversion applies; this catalog row carries no efficiency field, so the cell is cited through kcat_over_km_provenance only.

raw_value_unit_evidence: Table 1, enzyme row Wild-type, p-Nitrophenyl caprylate block: kcat/Km = 63 s^-1 mM^-1; Km = 28 uM; kcat = 1.7 s^-1.


organism: Streptococcus equi

substrate_name: vinyl propionate

sequence_source: UniProtKB release 2026_02 accession B5THG6

substrate: vinyl propionate


organism: Sulfolobus acidocaldarius

substrate_name: 4-nitrophenyl acetate

substrate: 4-nitrophenyl acetate


organism: Sulfurisphaera tokodaii

sequence_source: UniProtKB release 2026_02 accession Q976W8


organism: Bacillus velezensis


organism: Brevundimonas sp. QPT-2


organism: Escherichia coli

substrate_name: palmitoyl-CoA

assay_conditions_summary: pH 7.5; 310.15 K; cofactor CoA

substrate: palmitoyl-CoA


organism: Ferroplasma acidiphilum

sequence_source: UniProtKB:Q2PCE5 REST lookup 2026-06-19

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q2PCE5 REST lookup 2026-06-19 and applied mutation signature N248A/P256Q/E257G/S283F to reconstruct the assayed variant sequence.

proof_lines: pH 6.0, 35°C, mutant enzyme N248A/P256Q/E257G/S283F


organism: Homo sapiens

proof_lines:
isoform hCE-2, pH 8.0, 37°C
isoform hCE-1, pH 8.0, 37°C
enzyme from liver cytosol


substrate_name: irinotecan

proof_lines: mutant hCE1m6, ratio kcat/Km is 19.8 per min and mM

substrate: irinotecan


mutation_type: frameshift


sequence_source: UniProtKB:P23141 REST lookup 2026-06-19

sequence_variant_note: Resolved canonical sequence from UniProtKB:P23141 REST lookup 2026-06-19 and applied mutation signature G143E to reconstruct the assayed variant sequence.


proof_lines: recombinant refolded His-taggged mutant S76N, pH 7.5, 37°C


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: recombinant refolded His-taggged wild-type enzyme, pH 7.5, 37°C

proof_lines: wild-type, ratio kcat/Km is 0.28 per min and mM


proof_lines: recombinant refolded His-taggged mutant A270S, pH 7.5, 37°C


proof_lines: recombinant refolded His-taggged mutant D204E, pH 7.5, 37°C


substrate_name: (R)-propranolyl 2-methylbutanoate

sequence_source: UniProtKB:O00748 REST lookup 2026-06-19

proof_lines: enzyme hCE2, pH and temperature not specified in the publication

substrate: (R)-propranolyl 2-methylbutanoate


organism: Klebsiella sp.


organism: Lacticaseibacillus casei

sequence_source: UniProtKB:Q8KT44 direct accession lookup 2026-06-19


substrate_name: 4-nitrophenyl laurate

substrate: 4-nitrophenyl laurate


organism: Lucilia cuprina

substrate_name: methyl laurate

sequence_source: UniProtKB:Q25252 direct accession lookup 2026-06-19

substrate: methyl laurate


organism: Macaca fascicularis

substrate_name: (S)-propranolyl 3-methylbutanoate

sequence_source: UniProtKB:A0A2K5V282 direct accession lookup 2026-06-19

proof_lines: enzyme mfCES2v3, pH and temperature not specified in the publication

substrate: (S)-propranolyl 3-methylbutanoate


organism: Mus musculus


organism: Oryctolagus cuniculus

substrate_name: fluorescein diacetate

sequence_source: UniProtKB:G1T7P3 direct accession lookup 2026-06-19

substrate: fluorescein diacetate


substrate_name: D-luciferin methyl ester

sequence_source: UniProtKB:P12337 direct accession lookup 2026-06-19

proof_lines:
isoform CES1, at pH 6.5 and 37Â°C
ratio kcat/Km is 180 per min and mM
at pH 7.4 and 37Â°C

substrate: D-luciferin methyl ester


organism: Pyrobaculum calidifontis

substrate_name: 4-nitrophenyl propanoate

sequence_source: UniProtKB:Q8NKS0 direct accession lookup 2026-06-19

substrate: 4-nitrophenyl propanoate


organism: Pyrobaculum sp.

substrate_name: 4-nitrophenyl butyrate

sequence_source: UniProtKB:G7VG08 direct accession lookup 2026-06-19

substrate: 4-nitrophenyl butyrate


organism: Pyrococcus furiosus

substrate_name: 4-methylumbelliferyl heptanoate

sequence_variant_note: Resolved canonical sequence from UniProtKB:Q8TZJ1 direct accession lookup 2026-06-19 and applied mutation signature W194A to reconstruct the assayed variant sequence.

substrate: 4-methylumbelliferyl heptanoate


substrate_name: 4-Methylumbelliferyl butyrate

sequence_variant_note: Resolved canonical sequence from UniProtKB:Q8TZJ1 direct accession lookup 2026-06-19 and applied mutation signature F198A to reconstruct the assayed variant sequence.

substrate: 4-Methylumbelliferyl butyrate


sequence_source: UniProtKB:Q8TZJ1 direct accession lookup 2026-06-19


organism: Rattus norvegicus

substrate_name: retinyl palmitate

proof_lines:
isozyme ES4, pH 8.0, 37°C
isozyme ES10, pH 8.0, 37°C
isozyme ES3, pH 8.0, 37°C

substrate: retinyl palmitate


sequence_source: UniProtKB:Q63108 direct accession lookup 2026-06-19


sequence_source: UniProtKB:Q64573 direct accession lookup 2026-06-19


organism: Sus scrofa

sequence_source: UniProtKB:F1RF16 direct accession lookup


organism: uncultured bacterium


organism: uncultured microorganism

substrate_name: methyl ferulate

substrate: methyl ferulate


organism: Vulcanisaeta moutnovskia

sequence_source: UniProtKB:F0QXN6 direct accession lookup


organism: Acetivibrio thermocellus

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


organism: Acinetobacter lwoffii


organism: Agrobacterium tumefaciens


organism: Alicyclobacillus acidocaldarius

substrate_name: 4-nitrophenyl hexadecanoate

substrate: 4-nitrophenyl hexadecanoate


proof_lines: pH 7.1, 50°C, mutant M211S/R215L lacking N-terminal 36 amino acids


proof_lines: wild-type enzyme and mutant M211S, pH 7.1, 70°C

proof_lines: pH 7.1, 50°C, wild-type lacking N-terminal 36 amino acids


substrate_name: 4-nitrophenyl tetradecanoate

proof_lines: mutants R215L and M211S/R215L, pH 7.1, 70°C

substrate: 4-nitrophenyl tetradecanoate


substrate_name: 4-nitrophenyl dodecanoate

substrate: 4-nitrophenyl dodecanoate


substrate_name: vinyl acetate

substrate: vinyl acetate


organism: Bacillus subtilis

substrate_name: 4-nitrophenyl acetate

substrate: 4-nitrophenyl acetate


substrate_name: trans-permethrin

substrate: trans-permethrin


substrate_name: 4-nitrophenyl valerate

substrate: 4-nitrophenyl valerate


organism: Bombyx mori

substrate_name: p-nitrophenyl butyrate

substrate: p-nitrophenyl butyrate


organism: Bos taurus

substrate_name: phenyl trimethyl acetate

proof_lines:
at 70°C
at 150°C

substrate: phenyl trimethyl acetate


organism: Bradysia odoriphaga

substrate_name: 2-naphthyl acetate

assay_conditions_summary: pH 7

substrate: 2-naphthyl acetate


organism: Caenorhabditis elegans

substrate_name: 1-naphthyl acetate

substrate: 1-naphthyl acetate


organism: Calotropis procera

substrate_name: capric acid

substrate: capric acid


organism: Cavia porcellus

substrate_name: n-Octanoyl salicylate

substrate: n-Octanoyl salicylate


organism: Anoxybacillus sp.

substrate_name: 4-nitrophenyl butyrate

substrate: 4-nitrophenyl butyrate


organism: Arabidopsis thaliana

substrate_name: Unknown

proof_lines: AtMES1, pH and temperature not specified in the publication

proof_lines: AtMES9, pH and temperature not specified in the publication

substrate: Unknown


organism: Archaeoglobus fulgidus

substrate_name: tripropionin

substrate: tripropionin


proof_lines:
80°C, pH 8.0, wild-type enzyme
pH 8.0, 80°C, wild-type enzyme


substrate_name: (S)-ketoprofen ethyl ester

substrate: (S)-ketoprofen ethyl ester


organism: Arthrobacter globiformis

substrate_name: (+)-trans-ethyl crysanthemate

substrate: (+)-trans-ethyl crysanthemate


organism: Aspergillus nomiae

substrate_name: 4-nitrophenyl propionate

substrate: 4-nitrophenyl propionate


organism: Cereibacter sphaeroides

substrate_name: 4-nitrophenyl octanoate

assay_conditions_summary: pH 8.3; 303.15 K; buffer 2.5 mM Bicine

proof_lines: in 2.5 mM Bicine buffer, pH 8.3, at 30°C

substrate: 4-nitrophenyl octanoate


organism: Culex quinquefasciatus


organism: Culex sp.

substrate_name: malathion

substrate: malathion


organism: Enterobacter cloacae

substrate_name: propyl paraben

substrate: propyl paraben


organism: Equus caballus

substrate_name: phenylbutyrate

substrate: phenylbutyrate


organism: Escherichia coli

substrate_name: 4-nitrophenyl laurate

substrate: 4-nitrophenyl laurate


organism: Galeocerdo cuvier

substrate_name: methyl butyrate

substrate: methyl butyrate


organism: Gallus gallus

substrate_name: 2-Nitrophenyl butyrate

substrate: 2-Nitrophenyl butyrate


organism: Geobacillus stearothermophilus

substrate_name: p-nitrophenyl caproate

substrate: p-nitrophenyl caproate


substrate_name: 4-nitrophenyl butanoate

substrate: 4-nitrophenyl butanoate


organism: Homo sapiens

substrate_name: trandolapril

proof_lines: wild type enzyme, in PBS containing 10 mM HEPES, pH 7.4, at 37°C

substrate: trandolapril


organism: Lactobacillus delbrueckii subsp. lactis

substrate_name: 4-nitrophenyl decanoate

substrate: 4-nitrophenyl decanoate


organism: Locusta migratoria

substrate_name: 2-naphthyl acetate

substrate: 2-naphthyl acetate


organism: Malus domestica

substrate_name: 4-yethylumbelliferyl acetate

substrate: 4-yethylumbelliferyl acetate


organism: Microbacterium sp.

substrate_name: isopropyl cetraxate

substrate: isopropyl cetraxate


organism: Micrococcus sp.

substrate_name: monopropyl phthalate

proof_lines:
isoform E2, pH 7.5, 30°C
isoform E1, pH 7.5, 30°C

substrate: monopropyl phthalate


organism: Mus musculus


organism: Mycobacterium tuberculosis

substrate_name: 4-methylumbelliferyl butanoate

substrate: 4-methylumbelliferyl butanoate


organism: Myzus persicae

substrate_name: 1-naphthyl acetate

substrate: 1-naphthyl acetate


organism: Niallia circulans

substrate_name: 4-nitrophenyl octanoate

substrate: 4-nitrophenyl octanoate


organism: Oryctolagus cuniculus

assay_conditions_summary: pH 7.4; 310.15 K; buffer 50 mM HEPES

proof_lines:
pH 7.4, 37°C
in 50 mM HEPES buffer, pH 7.4, at 37°C


organism: Ovis aries

substrate_name: 4-nitrophenyl butyrate

substrate: 4-nitrophenyl butyrate


organism: Paenarthrobacter nitroguajacolicus

substrate_name: phenyl acetate

substrate: phenyl acetate


organism: Pseudomonas aeruginosa


organism: Pseudomonas aeruginosa PAO1

substrate_name: Alpha-naphthyl acetate

substrate: Alpha-naphthyl acetate


organism: Psychrobacter sp.


organism: Rhizobium viscosum

substrate_name: Glucose pentaacetate

substrate: Glucose pentaacetate


organism: Saccharolobus shibatae

substrate_name: 4-nitrophenyl acetate

substrate: 4-nitrophenyl acetate


organism: Saccharolobus solfataricus


organism: Spodoptera exigua

substrate_name: geranyl acetate

substrate: geranyl acetate


organism: Spodoptera littoralis

substrate_name: (Z)-3-hexenyl acetate

assay_conditions_summary: pH 7.4; 301.15 K; buffer 20 mM Tris

proof_lines: non-linear fitting, in 20 mM Tris buffer (pH 7.4), at 28°C

proof_lines: Lineweaver-Burk method, in 20 mM Tris buffer (pH 7.4), at 28°C

substrate: (Z)-3-hexenyl acetate


organism: Streptomyces lividans


organism: Thalassobacillus sp.


organism: Thermobifida fusca

substrate_name: cyclic poly(ethylene terephthalate) trimer


organism: Thermotoga maritima

substrate_name: p-nitrophenyl acetate

substrate: p-nitrophenyl acetate


organism: Tribolium castaneum

substrate_name: malathion

proof_lines:
non-specific enzyme, pH 6.9, 25°C
malathion-specific enzyme, pH 6.9, 25°C

substrate: malathion


organism: Triticum aestivum


organism: uncultured bacterium

proof_lines: at 35°C in 100 mM sodium phosphate (pH 7.0)


proof_lines: recombinant wild-type enzyme, pH 8.5, 30°C


substrate_name: 4-methylumbelliferyl acetate

substrate: 4-methylumbelliferyl acetate


organism: uncultured microorganism

sequence_source: UniProtKB A0A8F1NJR0; NCBI Protein QWQ57436.1; EMBL MW804671.1

proof_lines:
at pH 8.2 and 50°C
at pH 8.0 and 30°C


organism: Xeropicta derbentina


enzyme_display_name: protein-arginine deiminase

organism: Homo sapiens

substrate_name: benzoyl-L-arginine amide

source_license: CC BY 4.0; CC BY-NC-ND 4.0

sequence_source: UniProtKB REST Q9UM07 canonical sequence

proof_lines:
37°C
pH 7.5, 37°C
at pH 7.5 and 37°C

source_databases_merged: brenda skid

enzyme: protein-arginine deiminase

substrate: benzoyl-L-arginine amide


substrate_name: benzoyl-L-Arg ethyl ester

sequence_source: UniProtKB REST Q9ULC6 canonical sequence

proof_lines: pH 7.6, 55°C, peptidylarginine deiminase 1

substrate: benzoyl-L-Arg ethyl ester


sequence_source: UniProtKB REST Q9ULW8 canonical sequence

proof_lines: pH 7.6, 55°C, peptidylarginine deiminase 3


substrate_name: Nalpha-benzoyl-L-arginine

sequence_source: UniProtKB REST Q9Y2J8 canonical sequence

proof_lines:
at pH 7.6 and 37°C
Raw SKiD Env: Temp=37°C, pH=7.6

substrate: Nalpha-benzoyl-L-arginine


substrate_name: Nalpha-benzoyl-L-arginine methyl ester

source_db: oed

kcat_over_km_origin: calculated

substrate: Nalpha-benzoyl-L-arginine methyl ester

source: oed


substrate_name: Nalpha-benzoyl-L-arginine amide

substrate: Nalpha-benzoyl-L-arginine amide


substrate_name: N-alpha-benzoyl-L-arginine ethyl ester

sequence_variant_note: Resolved canonical sequence from UniProtKB REST Q9Y2J8 canonical sequence with reviewed R347A substitution applied and applied mutation signature R347A to reconstruct the assayed variant sequence.

substrate: N-alpha-benzoyl-L-arginine ethyl ester


sequence_variant_note: Resolved canonical sequence from UniProtKB REST Q9Y2J8 canonical sequence with reviewed D125A substitution applied and applied mutation signature D125A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB REST Q9Y2J8 canonical sequence with reviewed Q350A substitution applied and applied mutation signature Q350A to reconstruct the assayed variant sequence.

proof_lines:
mutant Q350A, pH 7.6, 37°C
pH 7.6, 37°C, mutant enzyme Q350A


sequence_source: UniProtKB REST Q9Y2J8 canonical sequence with reviewed D125A substitution applied

proof_lines:
mutant D125A, pH 7.6, 37°C
pH 7.6, 37°C, mutant enzyme D125A


sequence_variant_note: Resolved canonical sequence from UniProtKB REST Q9Y2J8 canonical sequence with reviewed D166A substitution applied and applied mutation signature D166A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB REST Q9Y2J8 canonical sequence with reviewed D374A substitution applied and applied mutation signature D374A to reconstruct the assayed variant sequence.

proof_lines:
mutant D374A, pH 7.6, 37°C
pH 7.6, 37°C, mutant enzyme D374A


sequence_source: UniProtKB REST Q9Y2J8 canonical sequence with reviewed R347A substitution applied


sequence_variant_note: Resolved canonical sequence from UniProtKB REST Q9Y2J8 canonical sequence with reviewed D177A substitution applied and applied mutation signature D177A to reconstruct the assayed variant sequence.


sequence_source: UniProtKB REST Q9Y2J8 canonical sequence with reviewed D374A substitution applied


sequence_variant_note: Resolved canonical sequence from UniProtKB canonical FASTA Q9Y2J8 with D389A reconstructed from selected source mutation_signature and applied mutation signature D389A to reconstruct the assayed variant sequence.