organism: Aspergillus awamori

proof_lines: in 20% N,N-dimethyl formamide, 50 mM acetate buffer, pH 5, with 0.05 ml enzyme solution, at 37°C for 10 min


enzyme_display_name: feruloyl esterase

organism: Aspergillus flavus

substrate_name: methyl sinapinate

source_db: brenda

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

source_license: CC BY 4.0

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines:
pH 5.5, 40°C, recombinant enzyme
pH 6.0, 58°C

enzyme: feruloyl esterase

substrate: methyl sinapinate

source: brenda


organism: Aspergillus nidulans

substrate_name: methyl caffeate

kcat_display: Not reported

substrate: methyl caffeate


organism: Aspergillus ochraceus


organism: Aspergillus oryzae


organism: Bacillus amyloliquefaciens

substrate_name: methyl p-coumarate

substrate: methyl p-coumarate


organism: Bacillus pumilus

substrate_name: methyl sinapate

substrate: methyl sinapate


organism: Butyrivibrio proteoclasticus

substrate_name: p-nitrophenyl acetate

assay_conditions_summary: pH 7.2; 310.15 K; buffer 100 mM phosphate

proof_lines: in 100 mM phosphate buffer, pH 7.2, at 37°C

substrate: p-nitrophenyl acetate


organism: Caldanaerobacter subterraneus subsp. tengcongensis

substrate_name: methyl ferulate

assay_conditions_summary: pH 8; 338.15 K; buffer 50 mM sodium phosphate

proof_lines: at 65°C, in 50 mM sodium phosphate buffer, pH 8.0

substrate: methyl ferulate


enzyme_display_name: acetylcholinesterase

organism: Rachycentron canadum

substrate_name: acetylthiocholine

assay_conditions_summary: pH 8; buffer 0.5 M Tris-HCl

proof_lines: in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication

enzyme: acetylcholinesterase

substrate: acetylthiocholine


organism: Rattus norvegicus

substrate_name: acetylthiocholine iodide

proof_lines: pH 8, 37°C, brain, membrane-bound form, component I

proof_lines: pH 8, 37°C, erythrocyte, membrane-bound form, component I

proof_lines: pH 8, 37°C, brain, soluble form, component II

substrate: acetylthiocholine iodide


organism: Rhopalosiphum padi

substrate_name: butylthiocholine

substrate: butylthiocholine


organism: Rhynchophorus ferrugineus

assay_conditions_summary: pH 8

proof_lines:
isoform AChEIIb, pH 8, 37Â°C
isoform AChEIIIb, pH 8, 37Â°C


organism: Lactarius hatsudake


organism: Lactobacillus acidophilus


organism: Neurospora crassa

substrate_name: methyl 4-coumarate

substrate: methyl 4-coumarate


organism: Penicillium expansum

substrate_name: methyl coumarate

proof_lines:
p-coumaroyl esterase
37°C, pH 6.0
pH 5.6, 37°C

substrate: methyl coumarate


organism: rumen bacterium

substrate_name: n-decyl ferulate

substrate: n-decyl ferulate


organism: rumen microorganism

proof_lines: transesterification, immobilized enzyme, pH not specified in the publication, 40°C

proof_lines: transesterification, free enzyme, pH not specified in the publication, 40°C

proof_lines: hydrolysis, free enzyme, pH not specified in the publication, 40°C


organism: Russula virescens


organism: Schizophyllum commune


organism: Thermoclostridium stercorarium


organism: Thermothelomyces heterothallicus

proof_lines: transesterification in a n-hexane/t-butanol/water solvent system, 35°C


organism: Thermothelomyces thermophilus


organism: uncultured bacterium


organism: Cellulosilyticum ruminicola


organism: Eleusine coracana

substrate_name: p-nitrophenylferulate

substrate: p-nitrophenylferulate


organism: Escherichia coli

substrate_name: p-coumaric acid methyl ester

substrate: p-coumaric acid methyl ester


organism: Fusarium proliferatum


organism: Sorangium cellulosum

assay_conditions_summary: pH 7

proof_lines: pH 7.0, temperature not specified in the publication


organism: Streptomyces olivochromogenes


organism: Streptomyces sp.


organism: Streptomyces werraensis


organism: Pleurotus eryngii


organism: Pleurotus giganteus


organism: Pleurotus sapidus


organism: Pseudomonas fluorescens


organism: Talaromyces funiculosus


organism: Talaromyces pinophilus

substrate_name: ferulic acid methyl ester

substrate: ferulic acid methyl ester


organism: Talaromyces stipitatus

substrate_name: methyl cinnamate

proof_lines:
hydrolysis
pH 6.0-7.0, 60°C

substrate: methyl cinnamate


organism: Thermochaetoides dissita

substrate_name: 4-nitrophenyl butanoate

substrate: 4-nitrophenyl butanoate


enzyme_display_name: alpha-glucosidase

organism: Entamoeba histolytica

substrate_name: Unknown

km_display: Not reported

ki_unit: mM

proof_lines: versus 4-methylumbelliferyl alpha-D-glucoside, pH 5.5, 45°C

enzyme: alpha-glucosidase

substrate: Unknown


organism: Equus caballus

substrate_name: maltotetraose

substrate: maltotetraose


organism: Erwinia rhapontici


organism: Escherichia coli

substrate_name: maltose

substrate: maltose


organism: Brassica oleracea

proof_lines: and a second alpha-glucosidase with Km of 0.0086 mM

proof_lines: and a second alpha-glucosidase with Km of 0.3 mM


organism: Chilo suppressalis

substrate_name: p-nitrophenyl-alpha-D-glucopyranoside

proof_lines:
salivary gland
midgut

substrate: p-nitrophenyl-alpha-D-glucopyranoside


organism: Drosophila melanogaster

substrate_name: sucrose

proof_lines:
alpha-glucosidase III
alpha-glucosidase II
alpha-glucosidase I

substrate: sucrose


organism: Dysdercus peruvianus


organism: uncultured microorganism

substrate_name: methyl sinapate

proof_lines:
recombinant enzyme, pH 8.0, 37°C
pH 5.0, 30°C

substrate: methyl sinapate


organism: Zea mays

substrate_name: methyl ferulate

substrate: methyl ferulate


organism: Geobacillus stearothermophilus


organism: Geobacillus thermodenitrificans


organism: Glycine max

proof_lines:
alpha-glucoamylase II
glucoamylase I


organism: Homo sapiens

substrate_name: resorufin alpha-D-glucopyranoside

proof_lines: catalytic subunit ntMGAM, pH and temperature not specified in the publication

proof_lines: catalytic subunit ctMGAMN2, pH and temperature not specified in the publication

substrate: resorufin alpha-D-glucopyranoside


organism: Bacillus subtilis

substrate_name: p-nitrophenyl-alpha-D-glucoside

substrate: p-nitrophenyl-alpha-D-glucoside


organism: Bacillus thuringiensis serovar kurstaki

substrate_name: 4-nitrophenyl alpha-D-glucopyranoside

substrate: 4-nitrophenyl alpha-D-glucopyranoside


organism: Beta vulgaris


organism: Bifidobacterium longum subsp. Longum


organism: Aureobasidium pullulans


organism: Bacillus cereus


organism: Bacillus licheniformis

proof_lines: free enzyme, pH not specified in the publication, temperature not specified in the publication

proof_lines: immobilized enzyme entrapped in agar, pH not specified in the publication, temperature not specified in the publication


organism: Bacillus sp. (in: firmicutes)


organism: Apis mellifera

substrate_name: kojibiose

substrate: kojibiose


organism: Aplysia fasciata


organism: Aspergillus niger

substrate_name: maltohexaose

proof_lines:
soluble
DP = 17
intracellular enzyme

substrate: maltohexaose


organism: Aspergillus niveus

substrate_name: p-nitrophenyl alpha-D-glucopyranoside

substrate: p-nitrophenyl alpha-D-glucopyranoside


organism: Oryza sativa

proof_lines:
alpha-glucosidase I
alpha-glucosidase II
G17, alpha-glucosidase I


organism: Paecilomyces variotii


organism: Panicum miliaceum


organism: Parageobacillus thermoglucosidasius


enzyme_display_name: aryldialkylphosphatase

organism: Oryctolagus cuniculus

substrate_name: diethyl paraoxon

assay_conditions_summary: pH 7.4; buffer 20 mM Tris-HCl

proof_lines: in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication

enzyme: aryldialkylphosphatase

substrate: diethyl paraoxon


organism: Mortierella alliacea


organism: Mucor javanicus


organism: Mucor racemosus

substrate_name: maltotriose

substrate: maltotriose


substrate_name: maltopentaose

substrate: maltopentaose


organism: Xanthomonas campestris

substrate_name: Vanillin

substrate: Vanillin


organism: Zea mays

substrate_name: turanose

substrate: turanose


organism: Thermococcus sp.

proof_lines: enzyme immobilized on oxirane acrylic beads


organism: Thermomonospora curvata


organism: Trichoderma viride


organism: Vigna radiata

substrate_name: amylose

proof_lines:
alpha-glucosidase I and II-2
alpha-glucosidase I
alpha-glucosidase II-1

substrate: amylose


organism: Talaromyces purpureogenus

substrate_name: maltose

substrate: maltose


organism: Tetrahymena pyriformis


organism: Thermochaetoides thermophila


organism: Phaffia rhodozyma

substrate_name: soluble starch

substrate: soluble starch


organism: Purpureocillium lilacinum

proof_lines:
pH 5.0, 50°C
soluble substrate, pH 5.0, 50°C


organism: Pyrococcus furiosus

substrate_name: p-nitrophenyl-alpha-D-glucopyranoside

substrate: p-nitrophenyl-alpha-D-glucopyranoside


organism: Quesada gigas

substrate_name: maltoheptaose

substrate: maltoheptaose


organism: Rattus norvegicus


organism: Rhizobium sp.

substrate_name: 4-nitrophenyl alpha-D-glucoside

substrate: 4-nitrophenyl alpha-D-glucoside


organism: Saccharolobus solfataricus


organism: Saccharomyces cerevisiae

proof_lines: pH 7.0, 30°C, recombinant wild-type enzyme


proof_lines:
alpha-glucosidase III
alpha-glucosidase I
alpha-glucosidase II


organism: Saccharomyces pastorianus


organism: Solanum tuberosum


organism: Sus scrofa

proof_lines:
alpha-glucosidase IIa
alpha-glucosidase IIb


enzyme_display_name: beta-glucosidase

organism: Daldinia eschscholtzii

proof_lines: pH and temperature not specified in the publication

enzyme: beta-glucosidase


organism: Hanseniaspora uvarum

substrate_name: 4-nitrophenyl beta-D-glucoside

substrate: 4-nitrophenyl beta-D-glucoside


enzyme_display_name: funoran endo-beta-hydrolase

organism: Wenyingzhuangia aestuarii

substrate_name: funoran

enzyme: funoran endo-beta-hydrolase

substrate: funoran


organism: Hordeum vulgare


organism: Lactobacillus acidophilus

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Lathyrus oleraceus

proof_lines:
alpha-glucosidase 1
alpha-glucosidase 2
alpha-glucosidase 3


organism: Levilactobacillus brevis


enzyme_display_name: peptidyl-dipeptidase A

organism: Alitta virens

substrate_name: benzoyl-Gly-His-Leu

enzyme: peptidyl-dipeptidase A

substrate: benzoyl-Gly-His-Leu


organism: Bacillus pumilus


organism: Bos taurus

proof_lines:
pH 7.5, 25°C, N-domain from lung enzyme
pH 7.5, 25°C, enzyme from lung
pH 7.5, 25°C, enzyme from testis


organism: Cavia porcellus

substrate_name: angiotensin I

proof_lines:
lung enzyme
serum enzyme

substrate: angiotensin I


organism: Oryctolagus cuniculus

assay_conditions_summary: pH 7.5; 310.15 K; buffer 50 mM Tris-HCl

proof_lines:
enzyme from lung
enzyme from testis
enzyme from testis and lung


organism: Pseudomonas sp.

substrate_name: Unknown

substrate: Unknown


organism: Rattus norvegicus

proof_lines: pH 7.5, 37°C, enzyme from brain, 200 mM NaCl

proof_lines: pH 7.5, 37°C, enzyme from brain, 10 mM NaCl

proof_lines: pH 7.5, 37°C, enzyme from testis, 10 mM NaCl


organism: Struthio camelus


organism: Drosophila sp. (in: flies)

proof_lines:
35°C, pH 7.0, 0 mM NaCl
35°C, pH 8.0, 0 mM NaCl
35°C, pH 8.0, 100 mM NaCl


organism: Gallus gallus

substrate_name: hippuryl-His-Leu

proof_lines:
detergent-solubilized enzyme
trypsin-extracted enzyme

substrate: hippuryl-His-Leu


organism: Homo sapiens

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: 37°C, pH 7.0, full-length mutant containing only a functional N-domain catalytic site

proof_lines: 37°C, pH 7.0, full-length mutant containing only a functional C-domain catalytic site


organism: Locusta migratoria

substrate_name: NKLKPSQWISL

substrate: NKLKPSQWISL


organism: Sus scrofa

proof_lines:
pH 7.5, 25°C
pH 7.5, 25°C, cobalt-ACE
pH 6, 0.1 M NaCl


organism: Ucides cordatus

substrate_name: Abz-LFK(Dnp)-OH

substrate: Abz-LFK(Dnp)-OH


enzyme_display_name: chymotrypsin

substrate_name: benzoyl-Gly-4-guanidinophenyl ester

assay_conditions_summary: pH 7.6; 298.15 K; buffer saline, pH 7

proof_lines:
25°C, pH 7.6
transesterfication in 10% ethanol
21°C, pH 8.3, 20 mM CaCl2

enzyme: chymotrypsin

substrate: benzoyl-Gly-4-guanidinophenyl ester


organism: Camelus bactrianus

substrate_name: acetyl-Trp ethyl ester

substrate: acetyl-Trp ethyl ester


organism: Camelus dromedarius

substrate_name: N-acetyl-L-leucine methyl ester

substrate: N-acetyl-L-leucine methyl ester


organism: Carassius auratus

proof_lines:
chymotrypsin B, at pH 7.5 and 37°C
chymotrypsin A, at pH 7.5 and 37°C


organism: Diatraea saccharalis


organism: Gadus morhua

substrate_name: N-benzoyl-Tyr p-nitroanilide

proof_lines: enzyme form A and enzyme form B, at pH 10°C

substrate: N-benzoyl-Tyr p-nitroanilide


organism: Musca domestica

proof_lines: pH 8.5, temperature not specified in the publication


organism: Panulirus interruptus


organism: Pterygoplichthys disjunctivus


organism: Sardinops caeruleus

substrate_name: succinyl-Ala-Ala-Pro-Phe-p-nitroanilide

proof_lines:
at 30°C, chymotrypsin II
at 30°C, chymotrypsin I

substrate: succinyl-Ala-Ala-Pro-Phe-p-nitroanilide


organism: Sardinops sagax

substrate_name: N-succinyl-L-Ala-Ala-Pro-p-nitroanilide

proof_lines: in 50 mM Tris-HCl pH 8.0 with 10 mM CaCl2, at 30 °C

substrate: N-succinyl-L-Ala-Ala-Pro-p-nitroanilide


organism: Sepia officinalis


organism: Struthio camelus


organism: synthetic construct

assay_conditions_summary: 310.15 K; buffer 50 mM Tris-HCl

proof_lines: at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl


organism: Tenebrio molitor


enzyme_display_name: tissue kallikrein

organism: Homo sapiens

substrate_name: Abz-KLFSSEQ-EDDnp

assay_conditions_summary: pH 7.5; 310.15 K; buffer 20 mM Tris/HCl

proof_lines:
at pH 7.5 and 37°C
pH 9.0, 30°C
pH 9.0, 37°C

enzyme: tissue kallikrein

substrate: Abz-KLFSSEQ-EDDnp


organism: Mus musculus


organism: Rattus norvegicus


organism: Sus scrofa

substrate_name: N-alpha-tosyl-L-arginine methyl ester

proof_lines:
alpha-kallikrein, pH 8.0, 25°C
pH 9.0, 25°C
pH 9.0, 30°C

substrate: N-alpha-tosyl-L-arginine methyl ester


enzyme_display_name: furin

organism: Saccharomyces cerevisiae

enzyme: furin


enzyme_display_name: trypsin

organism: Bos taurus

substrate_name: M-diarginyl insulin

assay_conditions_summary: pH 8; 310.15 K; buffer 67 mM sodium phosphate

proof_lines:
pH 8.0, 37°C
at 5°C
gamma-trypsin

enzyme: trypsin

substrate: M-diarginyl insulin


organism: Diatraea saccharalis

proof_lines: at 30°C in 100 mM Tris-HCl buffer pH 8.5


proof_lines:
pH 8.0, 37°C, wild-type enzyme
wild-type enzyme, pH 9
wild-type enzyme, pH 8


substrate_name: FITC-casein

substrate: FITC-casein


enzyme_display_name: cathepsin L

organism: Fasciola gigantica

proof_lines: wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C

enzyme: cathepsin L


organism: Gallus gallus


assay_conditions_summary: pH 5.5; 310.15 K; buffer 50 mM Mes

proof_lines: 50 mM Na-acetate, 100 mM NaCl, 2.5 mM EDTA, pH 5.5, 37°C

proof_lines: in 50 mM Mes buffer (pH 6.0) containing 5 mM dithiothreitol, at 37°C

proof_lines: recombinant enzyme expressed Escherichia coli


organism: Hypophthalmichthys molitrix


enzyme_display_name: ribulose-bisphosphate carboxylase

organism: Agave victoriae-reginae

substrate_name: D-ribulose 1,5-bisphosphate

proof_lines: in 100 mM Bicine-NaOH pH 8.0, 20 mM MgCl2, at 25°C

enzyme: ribulose-bisphosphate carboxylase

substrate: D-ribulose 1,5-bisphosphate


organism: Helianthus annuus


organism: Nicotiana tabacum

proof_lines: enzyme assembled with small subunit isoform RbcS-S1, pH 8.0, 25°C

proof_lines: enzyme assembled with small subunit isoform RbcS-S5, pH 8.0, 25°C

proof_lines: enzyme assembled with small subunit isoform RbcS-S2, pH 8.0, 25°C


organism: Pseudomonas sp.

proof_lines: recombinant mutant H57betaS/H70betaS/M165alphaS/I176betaS, pH 8.0, 37°C

proof_lines: recombinant mutant H57betaS/H70betaS/L176betaK, pH 8.0, 37°C

proof_lines: recombinant mutant H57betaS/H70betaS/F58betaD, pH 8.0, 37°C


organism: Pseudomonas sp. 130

substrate_name: glutaryl-7-aminocephalosporanic acid

substrate: glutaryl-7-aminocephalosporanic acid


organism: Spodoptera frugiperda

proof_lines: 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C


organism: Takifugu rubripes


organism: Toxoplasma gondii

substrate_name: Benzyloxycarbonyl-Lys-Arg

substrate: Benzyloxycarbonyl-Lys-Arg


organism: Trypanosoma brucei

proof_lines: in the presence of 0.001 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C

proof_lines: in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 37°C


organism: Brevibacillus laterosporus


organism: Brevundimonas diminuta


organism: Pseudomonas nitroreducens

substrate_name: L-gamma-glutamyl p-nitroanilide

substrate: L-gamma-glutamyl p-nitroanilide


substrate_name: glutaryl-GGGGGK

proof_lines: at 37°C, pH not specified in the publication

substrate: glutaryl-GGGGGK


organism: Agama stellio stellio


organism: Apostichopus japonicus


organism: Cavia porcellus


organism: Cyprinus carpio


organism: Mus musculus

substrate_name: Unknown

substrate: Unknown


organism: Oncorhynchus keta


organism: Oryctolagus cuniculus


organism: Rattus norvegicus


organism: Cryptosporidium parvum


organism: Homo sapiens

substrate_name: pro-MT-MMP 3 protein

proof_lines:
pH 7.0, 37°C
pH 7.0, 37°C, presence of Mg2+
pH 7.0, 37°C, absence of Mg2+

substrate: pro-MT-MMP 3 protein


organism: Mammalia


enzyme_display_name: catechol oxidase

organism: Solanum tuberosum

substrate_name: catechol

km_unit: mM

enzyme: catechol oxidase

substrate: catechol


enzyme_display_name: ubiquinol oxidase (non-electrogenic)

organism: Trypanosoma brucei brucei

verification_status: unverified

protein_accession_database: UniProt

source_license: CC BY 4.0; CC BY-NC-ND 4.0

assay_conditions_summary: pH 7.5; 298.15 K; buffer conditions

proof_lines:
pH 7.5, 25°C
pH 7.4, 25°C
Raw SKiD Env: Temp=25°C, pH=7.5

source_databases_merged: brenda skid

enzyme: ubiquinol oxidase (non-electrogenic)

status: unverified


organism: Marinomonas mediterranea

substrate_name: Syringaldazine; O2

kcat_over_km_origin: calculated

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

sequence_source: UniProtKB direct accession lookup Q9KJB8 via rest.uniprot.org/uniprotkb/Q9KJB8.json on 2026-06-05

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

data_origin: experimental

substrate: Syringaldazine; O2


organism: Prunus armeniaca

substrate_name: O2; Catechol

sequence_source: UniProtKB direct accession lookup O81103 via rest.uniprot.org/uniprotkb/O81103.json on 2026-06-05

substrate: O2; Catechol


enzyme_display_name: laccase

organism: Bacillus amyloliquefaciens

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

enzyme: laccase


organism: Bacillus altitudinis


enzyme_display_name: grixazone synthase

organism: Streptomyces griseus

enzyme: grixazone synthase


organism: Acinetobacter baumannii

substrate_name: O2; Guaiacol

substrate: O2; Guaiacol


organism: Bacillus licheniformis


source_db: oed

source: oed


organism: Bacillus atrophaeus


substrate_name: Syringaldazine

substrate: Syringaldazine


organism: Bacillus pumilus


source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)


substrate_name: O2; Syringaldazine

substrate: O2; Syringaldazine


organism: Bacillus sp. HR03

mutation_type: substitution


organism: Bacillus subtilis


sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


substrate_name: Guaiacol; O2

substrate: Guaiacol; O2


organism: Bacteroides thetaiotaomicron


organism: Bacillus tequilensis


organism: Burkholderia cepacia


organism: Cerrena unicolor

source_db: uniprot

source: uniprot


substrate_name: syringaldazine

verification_status: verified

sequence: AIGPVADLHI

wild_type_sequence: AIGPVADLHI

substrate: syringaldazine

status: verified


substrate_name: ABTS (at 70 degrees Celsius)

substrate: ABTS (at 70 degrees Celsius)


organism: Campylobacter jejuni

sequence_source: Selected-batch preserved UniProtKB REST accession A0A0H3PBA4 evidence, with RCSB 3ZX1 and PMID 22127520 cross-check retained in the shared family context.


organism: Cyathus bulleri

substrate_name: guaiacol

substrate: guaiacol


organism: Escherichia coli

substrate_name: 2,6-Dimethoxyphenol; O2

substrate: 2,6-Dimethoxyphenol; O2


organism: Fomitopsis pinicola


sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.

proof_lines: SABIO-RK modifier species: laccase(Enzyme) mutant P444I


sequence_source: Selected-batch preserved canonical context: UniProtKB:S8FIE4 direct FASTA; reviewed Swiss-Prot laccase EC 1.10.3.2; PMID:34116754 reference.


organism: Galerina sp

substrate_name: ABTS

sequence: GPSTDLVIGNKGFDGGIDSAILR

wild_type_sequence: GPSTDLVIGNKGFDGGIDSAILR

substrate: ABTS


organism: Ganoderma lucidum


organism: Galerina sp. DSM 22662

substrate_name: O2; Syringic acid

substrate: O2; Syringic acid


organism: Klebsiella pneumoniae


organism: Lepiota magnispora

substrate_name: ABTS (at pH 4)

sequence: VTIGKEGTLT

wild_type_sequence: VTIGKEGTLT

assay_conditions_summary: pH 4

substrate: ABTS (at pH 4)


organism: Panus rudis


organism: Ochrobactrum sp.

sequence_source: UniProtKB exact accession A6X5N0 REST lookup, retrieved 2026-07-01


organism: Podospora anserina


organism: Pseudomonas putida


organism: Pleurotus ostreatus


organism: Pseudomonas sp.


sequence_source: UniProtKB exact accession Q12739 REST lookup, retrieved 2026-07-01


organism: Rhizobium meliloti


organism: Rhodobacter capsulatus

substrate_name: O2; 2,6-Dimethoxyphenol

substrate: O2; 2,6-Dimethoxyphenol


organism: Salmonella typhimurium


sequence_source: UniProtKB exact accession Q8ZRS2 REST lookup, retrieved 2026-07-01


organism: Stenotrophomonas maltophilia


organism: Streptomyces coelicolor


organism: Staphylococcus haemolyticus

sequence_source: UniProtKB REST direct lookup L7SV51 on 2026-05-30


organism: Streptomyces ipomoeae

sequence_source: UniProtKB REST canonical sequence for A3QVH2


organism: Streptomyces sp. C1

substrate_name: L-Dopa; O2

substrate: L-Dopa; O2


organism: Thermothelomyces thermophilus


organism: Streptomyces viridochromogenes

sequence_source: UniProtKB REST canonical sequence for J9S5G3


organism: Toxicodendron vernicifluum

substrate_name: ferrocyanide

substrate: ferrocyanide


organism: Thermus thermophilus

sequence_source: UniProtKB:F6DF14 FASTA; direct REST lookup on 2026-05-30


organism: Trametes cinnabarina

sequence_source: UniProtKB Q9UVQ2 REST canonical sequence


organism: Trametes sp. C30


sequence_source: UniProtKB:Q8NID5 direct lookup


organism: unknown prokaryotic organism


organism: Homo sapiens

substrate_name: NRH

assay_conditions_summary: cofactor NADH

substrate: NRH


enzyme_display_name: L-ascorbate peroxidase

organism: Citrus limon

smiles: OO

enzyme: L-ascorbate peroxidase


substrate_name: Syringaldazine; O2

substrate: Syringaldazine; O2


substrate_name: L-ascorbate

substrate: L-ascorbate


enzyme_display_name: o-aminophenol oxidase

organism: Streptomyces antibioticus

substrate_name: dioxygen

enzyme: o-aminophenol oxidase

substrate: dioxygen


organism: Crocus sativus


organism: Trametes versicolor

source_db: skid

source_license: CC BY-NC-ND 4.0

proof_lines:
Raw SKiD Env: Temp=30°C, pH=3
Raw SKiD Env: Temp=30°C, pH=3.4

source: skid


organism: Pisum sativum

substrate_name: L-Ascorbate

substrate: L-Ascorbate


substrate_name: H2O2; L-Ascorbate

substrate: H2O2; L-Ascorbate


organism: Solanum lycopersicum

substrate_name: cumene hydroperoxide

substrate: cumene hydroperoxide


organism: Mus musculus

substrate_name: H(2)O(2) (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0)

assay_conditions_summary: pH 7; buffer 0.1 M phosphate


enzyme_display_name: manganese peroxidase

organism: Moniliophthora roreri

substrate_name: veratryl alcohol

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

enzyme: manganese peroxidase

substrate: veratryl alcohol


organism: Cerrena unicolor

sequence_source: UniProt REST A0A7D5FUQ6 FASTA


substrate_name: Reactive Black 5

substrate: Reactive Black 5


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature A172W/A273T with inferred residue-numbering offset +19 to reconstruct the assayed variant sequence.


organism: Trypanosoma cruzi


sequence_variant_note: Resolved canonical sequence from UniProt V2XS39 FASTA, reconstructed with paper-to-UniProt residue offset +19 and applied mutation signature A172W/K168V with inferred residue-numbering offset +19 to reconstruct the assayed variant sequence.


organism: Phanerochaete chrysosporium

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R177E with inferred residue-numbering offset +21 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: manganese peroxidase(Enzyme) mutant R177E


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R177N with inferred residue-numbering offset +21 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: manganese peroxidase(Enzyme) mutant R177N


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R177D with inferred residue-numbering offset +21 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: manganese peroxidase(Enzyme) mutant R177D


enzyme_display_name: lignin peroxidase

organism: Phanerodontia chrysosporium

enzyme: lignin peroxidase


sequence: AVXHPGTXVSXAAXXXFIPL

wild_type_sequence: AVXHPGTXVSXAAXXXFIPL


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R177Q with inferred residue-numbering offset +21 to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: manganese peroxidase(Enzyme) mutant R177Q


smiles: [Ho]


enzyme_display_name: versatile peroxidase

organism: Pleurotus eryngii

enzyme: versatile peroxidase


sequence_source: UniProtKB:P11542 REST FASTA, fetched 2026-06-07


organism: Fasciola hepatica

substrate_name: t-Butylhydroperoxide

smiles: CC(C)(C)OO

substrate: t-Butylhydroperoxide


substrate_name: guaiacol

substrate: guaiacol


enzyme_display_name: dye decolorizing peroxidase

organism: Bacillus subtilis

enzyme: dye decolorizing peroxidase


organism: Irpex lacteus

sequence_source: UniProtKB:A0A2P1C6N4 plus RCSB 7D8M identity check


organism: Mycetinis scorodonius


enzyme_display_name: bromide peroxidase

organism: Corallina officinalis

substrate_name: bromide

enzyme: bromide peroxidase

substrate: bromide


organism: Pleurotus sapidus

proof_lines:
at pH 3.5 and 30°C
at pH 4.0 and 30°C


organism: Vibrio cholerae


enzyme_display_name: thioredoxin-dependent peroxiredoxin

organism: Aeropyrum pernix

sequence_source: UniProtKB:Q9Y9L0 REST lookup, release 2026_02

enzyme: thioredoxin-dependent peroxiredoxin


organism: Escherichia coli (strain K12)

substrate_name: H(2)O(2) (using glutathione Grx1 as electron donor)


organism: Escherichia coli

substrate_name: linoleic acid hydroperoxide

sequence_source: UniProtKB:P0AE52 REST lookup, release 2026_02

smiles: CCCCCC=CCC=CCCCCCCCC(=O)OO

substrate: linoleic acid hydroperoxide


enzyme_display_name: hydroperoxy fatty acid reductase

organism: Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

enzyme: hydroperoxy fatty acid reductase

substrate: NADPH


organism: Rhizobium etli


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)


substrate_name: H(2)O(2) (using glutathione as electron donor)


substrate: H(2)O(2) (using glutathione as electron donor)


enzyme_display_name: glutathione-dependent peroxiredoxin

organism: Bos taurus

enzyme: glutathione-dependent peroxiredoxin


enzyme_display_name: NADH-dependent peroxiredoxin

organism: Amphibacillus xylanus (strain ATCC 51415 / DSM 6626 / JCM 7361 / LMG 17667 / NBRC 15112 / Ep01)

enzyme: NADH-dependent peroxiredoxin


organism: Treponema pallidum

assay_conditions_summary: pH 7; 298.15 K; cofactor NADH


organism: Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)


organism: Haemophilus influenzae

proof_lines:
pH 7.8, 25°C
Raw SKiD Env: Temp=25°C, pH=7.8


organism: Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)


enzyme_display_name: lipoyl-dependent peroxiredoxin

organism: Xylella fastidiosa

enzyme: lipoyl-dependent peroxiredoxin


organism: Rattus norvegicus

substrate_name: dipalmitoyl phosphatidylcholine

substrate: dipalmitoyl phosphatidylcholine


enzyme_display_name: cytochrome-c peroxidase

organism: Nitrosomonas europaea

verification_status: corrected

enzyme: cytochrome-c peroxidase

status: corrected


enzyme_display_name: catalase

organism: Bacillus altitudinis

sequence_source: Selected-batch citation: UniProt REST uniprotkb/W6AYH3.json, accessed 2026-06-05; current accession check found the entry deleted.

enzyme: catalase


organism: Candida tropicalis


organism: Bacteroides fragilis


organism: Listeria seeligeri


organism: Homo sapiens


organism: Bordetella pertussis


organism: Geobacillus sp. B1


organism: Micrococcus luteus


organism: Proteus mirabilis


organism: Ruegeria pomeroyi DSS-3


organism: Pteris vittata

assay_conditions_summary: pH 7; buffer 50 mM potassium phosphate

proof_lines: in 50 mM potassium phosphate buffer (pH 7.0)


organism: Serratia marcescens

assay_conditions_summary: pH 7; 293.15 K; buffer 50 mM NaH2PO4-Na2HPO4

proof_lines: in 50 mM NaH2PO4-Na2HPO4 buffer (pH 7.0) at 20°C


enzyme_display_name: peroxidase

organism: Allium sativum

enzyme: peroxidase


organism: Synechocystis sp.


organism: Amycolatopsis sp.

substrate_name: H2O2; 2,4-Dichlorophenol

substrate: H2O2; 2,4-Dichlorophenol


organism: Armoracia rusticana

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

proof_lines:
at pH 6.0
Raw SKiD Env: Temp=-----, pH=6
Raw SKiD Env: Temp=30°C, pH=6.5

source_databases_merged: sabio_rk brenda skid


organism: Equus caballus


organism: Bos taurus

substrate_name: ethynylestradiol

proof_lines:
at 25°C, pH 6.0
at pH 6.0 and 22°C

substrate: ethynylestradiol


organism: Escherichia coli


organism: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)

substrate_name: lauric acid

substrate: lauric acid


enzyme_display_name: glutathione peroxidase

enzyme: glutathione peroxidase


organism: Saccharomyces cerevisiae

substrate_name: H2O2; Reduced glutathione

substrate: H2O2; Reduced glutathione


organism: Homo sapiens

substrate_name: glutathione

substrate: glutathione


organism: Mus musculus

assay_conditions_summary: pH 7; 298.15 K; buffer phosphate

substrate: H(2)O(2) (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0)


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)


enzyme_display_name: tryptophan 2,3-dioxygenase

substrate_name: L-1-Methyltryptophan; O2

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature H76S to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: tryptophan 2,3-dioxygenase(Enzyme) mutant TDO H76S, C-terminal His6-tag

enzyme: tryptophan 2,3-dioxygenase

substrate: L-1-Methyltryptophan; O2


substrate_name: O2; L-Tryptophan

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature Y45A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: tryptophan 2,3-dioxygenase(Enzyme) mutant Y45A (aa 19-388)

substrate: O2; L-Tryptophan


substrate_name: L-Tryptophan; O2

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R144A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: tryptophan 2,3-dioxygenase(Enzyme) mutant R144A (aa 19-388)

substrate: L-Tryptophan; O2


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S151A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: tryptophan 2,3-dioxygenase(Enzyme) mutant S151A (aa 19-388)


organism: Cupriavidus metallidurans


substrate_name: L-Tryptophan

substrate: L-Tryptophan


enzyme_display_name: glutaredoxin-dependent peroxiredoxin

enzyme: glutaredoxin-dependent peroxiredoxin


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature F140A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: tryptophan 2,3-dioxygenase(Enzyme) mutant F140A (aa 19-388)


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

substrate_name: H(2)O(2) (in strain FRD1)

substrate: H(2)O(2) (in strain FRD1)


organism: Trypanosoma cruzi

substrate_name: linolenic acid hydroperoxide

substrate: linolenic acid hydroperoxide


organism: Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

substrate_name: L-tryptophan

substrate: L-tryptophan


enzyme_display_name: linoleate 13S-lipoxygenase

organism: Triticum turgidum subsp. durum

substrate_name: arachidonic acid

sequence_source: UniProtKB:A0A7M3TWE5 direct REST lookup on 2026-06-07

enzyme: linoleate 13S-lipoxygenase

substrate: arachidonic acid


organism: Olea europaea

substrate_name: linolenic acid

sequence_source: UniProtKB:B6D1W5 direct REST lookup on 2026-06-07

substrate: linolenic acid


organism: Myxococcus xanthus


organism: Sus scrofa

smiles: CCCCCC=CCC=CCC=CCC=CCCCC(=O)[O-]


enzyme_display_name: 4-hydroxyphenylpyruvate dioxygenase

organism: Arabidopsis thaliana

substrate_name: O2; 4-Hydroxyphenylpyruvate

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

enzyme: 4-hydroxyphenylpyruvate dioxygenase

substrate: O2; 4-Hydroxyphenylpyruvate


enzyme_display_name: quercetin 2,3-dioxygenase

organism: Bacillus subtilis

substrate_name: Quercetin; O2

sequence_source: UniProtKB:P42106 direct REST lookup on 2026-06-15

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

enzyme: quercetin 2,3-dioxygenase

substrate: Quercetin; O2


organism: Frog


organism: Daucus carota

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


organism: Mycobacterium tuberculosis


organism: Pseudomonas sp.

substrate_name: phenylpyruvate

substrate: phenylpyruvate


organism: Rattus norvegicus


organism: Homo sapiens


enzyme_display_name: 1-hydroxy-2-naphthoate 1,2-dioxygenase

organism: Nocardioides sp.

enzyme: 1-hydroxy-2-naphthoate 1,2-dioxygenase


organism: Streptomyces avermitilis


enzyme_display_name: biphenyl-2,3-diol 1,2-dioxygenase

organism: Achromobacter sp.

enzyme: biphenyl-2,3-diol 1,2-dioxygenase


sequence_source: UniProtKB:B3VTL5 direct lookup; live TrEMBL entry B3VTL5_9BURK; sequence version 1; entry version 33; accessed 2026-06-06


organism: Bacillus sp. (in: firmicutes)

substrate_name: catechol

sequence_source: UniProtKB:Q8GR45 direct lookup; reviewed Swiss-Prot entry BPHC_GEOG3; sequence version 1; entry version 68; accessed 2026-06-06

substrate: catechol


organism: Geobacillus genomosp. 3

substrate_name: 2,3-dihydroxybiphenyl (at 60 degrees Celsius and pH 7.5)


enzyme_display_name: gentisate 1,2-dioxygenase

organism: Klebsiella pneumoniae

substrate_name: gentisate

enzyme: gentisate 1,2-dioxygenase

substrate: gentisate


organism: Martelella sp. AD-3


organism: Rhizorhabdus dicambivorans


organism: Ralstonia sp.

substrate_name: O2; Gentisate

substrate: O2; Gentisate


enzyme_display_name: arachidonate 8-lipoxygenase

organism: Plexaura homomalla

substrate_name: arachidonate

smiles: CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(=O)O

enzyme: arachidonate 8-lipoxygenase

substrate: arachidonate


organism: Sphingomonas sp.


enzyme_display_name: linoleate 11-lipoxygenase

organism: Gaeumannomyces graminis var. avenae

substrate_name: O2; alpha-Linolenate

sequence_source: UniProtKB reviewed accession Q8X151

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

enzyme: linoleate 11-lipoxygenase

substrate: O2; alpha-Linolenate


enzyme_display_name: 1,2-dihydroxynaphthalene dioxygenase

organism: Pseudomonas putida

substrate_name: 3-methylcatechol (at pH 5.5)

enzyme: 1,2-dihydroxynaphthalene dioxygenase

substrate: 3-methylcatechol (at pH 5.5)


enzyme_display_name: gallate dioxygenase

organism: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)

substrate_name: gallate

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

enzyme: gallate dioxygenase

substrate: gallate


enzyme_display_name: linoleate 9S-lipoxygenase

organism: Solanum lycopersicum

substrate_name: linoleate

sequence_source: UniProtKB P38415 direct accession lookup

smiles: CCCCCC=CCC=CCCCCCCCC(=O)[O-]

enzyme: linoleate 9S-lipoxygenase

substrate: linoleate


enzyme_display_name: 3-hydroxyanthranilate 3,4-dioxygenase

organism: Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

enzyme: 3-hydroxyanthranilate 3,4-dioxygenase


organism: Rattus norvegicus


organism: Sphingobium xenophagum

sequence_source: UniProtKB:P74836 reviewed Swiss-Prot entry NSAC_SPHXE, cross-checked against PMID 2050635, BRENDA EC 1.13.11.56 cross-reference, and BN6 strain evidence.


organism: Capsicum annuum

sequence_source: UniProtKB D3TTH9 direct accession lookup (canonical sequence already preserved in the selected batch)


enzyme_display_name: linolenate 9R-lipoxygenase

organism: Nostoc sp. PCC 7120 = FACHB-418

substrate_name: alpha-linolenic acid

enzyme: linolenate 9R-lipoxygenase

substrate: alpha-linolenic acid


enzyme_display_name: beta-carotene 15,15'-dioxygenase

organism: Mus musculus

substrate_name: beta-Carotene; O2

sequence_source: UniProtKB reviewed accession Q9JJS6 fetched by direct UniProt REST check on 2026-06-11

enzyme: beta-carotene 15,15'-dioxygenase

substrate: beta-Carotene; O2


organism: Candidatus Nitrosotalea devanaterra


enzyme_display_name: 2-aminophenol 1,6-dioxygenase

organism: Comamonas testosteroni

assay_conditions_summary: pH 8; 295.15 K; buffer 10 mM sodium phosphate

proof_lines: in 10 mM sodium phosphate buffer, at pH 8.0 and 22°C

enzyme: 2-aminophenol 1,6-dioxygenase


enzyme_display_name: linoleate 8R-lipoxygenase

organism: Aspergillus nidulans

substrate_name: palmitoleate

sequence_source: EBI Proteins API UniProt accession Q6RET3

smiles: CCCCCCC=CCCCCCCCC(=O)[O-]

enzyme: linoleate 8R-lipoxygenase

substrate: palmitoleate


organism: Nitrosotalea devaniterrae


organism: Pseudomonas sp.

substrate_name: 2-Aminophenol; O2

substrate: 2-Aminophenol; O2


enzyme_display_name: 2-hydroxyethylphosphonate dioxygenase

organism: Streptomyces viridochromogenes

sequence_source: UniProt REST direct accession lookup, Q5IW40, 2026-06-15

smiles: C(CP(=O)(O)O)O

enzyme: 2-hydroxyethylphosphonate dioxygenase


organism: Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)


organism: uncultured bacterium HF130_AEPn_1

sequence_source: UniProtKB D0E8I5 canonical sequence, already preserved in the selected batch.


enzyme_display_name: protocatechuate 4,5-dioxygenase

organism: Sphingobium sp.

substrate_name: protocatechuate

enzyme: protocatechuate 4,5-dioxygenase

substrate: protocatechuate


enzyme_display_name: oleate 10S-lipoxygenase

substrate_name: oleate

assay_conditions_summary: pH 9; 303.15 K; buffer paper note 'in 0.2 M sodium borate

proof_lines: in 0.2 M sodium borate buffer, pH 9.0, at 30°C

enzyme: oleate 10S-lipoxygenase

substrate: oleate


organism: Novosphingobium aromaticivorans


enzyme_display_name: nitronate monooxygenase

organism: Metarhizium brunneum

sequence_source: UniProtKB direct accession check: A0A6C0WVK2 unreviewed TrEMBL entry A0A6C0WVK2_9HYPO

enzyme: nitronate monooxygenase


enzyme_display_name: Oplophorus-luciferin 2-monooxygenase

organism: Oplophorus gracilirostris

substrate_name: coelenterazine

enzyme: Oplophorus-luciferin 2-monooxygenase

substrate: coelenterazine


sequence_source: UniProtKB direct accession check: A0A6C0WVH0 unreviewed TrEMBL entry A0A6C0WVH0_9HYPO


organism: Pseudomonas syringae

proof_lines:
pH 8.0, 25°C
Raw SKiD Env: Temp=25°C, pH=8


enzyme_display_name: catalase

organism: Brucella abortus

enzyme: catalase


smiles: O=C(O)CCC(=O)C(=O)O


enzyme_display_name: firefly luciferase

organism: Amydetes vivianii

substrate_name: D-firefly luciferin

enzyme: firefly luciferase

substrate: D-firefly luciferin


organism: Luciola cruciata

substrate_name: lauric acid

substrate: lauric acid


enzyme_display_name: inositol oxygenase

organism: Sporopachydermia lactativora

smiles: O=O

enzyme: inositol oxygenase


organism: Rattus norvegicus

substrate_name: Inositol

substrate: Inositol


enzyme_display_name: phenylalanine 2-monooxygenase

substrate_name: L-phenylalanine

sequence_source: UniProtKB Q5W9R9 direct REST lookup; reviewed Swiss-Prot entry PAO_PSESP

assay_conditions_summary: pH 7; 303.15 K; buffer 50 mM potassium phosphate

enzyme: phenylalanine 2-monooxygenase

substrate: L-phenylalanine


enzyme_display_name: gamma-butyrobetaine dioxygenase

enzyme: gamma-butyrobetaine dioxygenase


organism: Pseudomonas sp

substrate_name: L-Phe (for the oxygenation reaction)

substrate: L-Phe (for the oxygenation reaction)


enzyme_display_name: deacetoxyvindoline 4-hydroxylase

organism: Catharanthus roseus

enzyme: deacetoxyvindoline 4-hydroxylase


enzyme_display_name: deacetoxycephalosporin-C hydroxylase

organism: Streptomyces clavuligerus

substrate_name: cephalosporin G

enzyme: deacetoxycephalosporin-C hydroxylase

substrate: cephalosporin G


organism: Hapsidospora chrysogena


enzyme_display_name: procollagen-lysine 5-dioxygenase

organism: Gallus gallus

enzyme: procollagen-lysine 5-dioxygenase


enzyme_display_name: L-asparagine oxygenase

organism: Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

substrate_name: L-asparagine

enzyme: L-asparagine oxygenase

substrate: L-asparagine


enzyme_display_name: thymine dioxygenase

organism: Neurospora crassa

enzyme: thymine dioxygenase


enzyme_display_name: feruloyl-CoA 6-hydroxylase

organism: Arabidopsis thaliana

substrate_name: trans-feruloyl-CoA

assay_conditions_summary: pH 6.5; 303.15 K; cofactor CoA

enzyme: feruloyl-CoA 6-hydroxylase

substrate: trans-feruloyl-CoA


organism: Ruta graveolens

assay_conditions_summary: pH 7.2; 303.15 K; cofactor CoA


organism: Zea mays

assay_conditions_summary: pH 7; 303.15 K; buffer 100 mM potassium phosphate

proof_lines: in 100 mM potassium phosphate buffer (pH 7.0), at 30°C


enzyme_display_name: trans-4-coumaroyl-CoA 2'-hydroxylase

enzyme: trans-4-coumaroyl-CoA 2'-hydroxylase


enzyme_display_name: trimethyllysine dioxygenase

enzyme: trimethyllysine dioxygenase


substrate_name: trimethyllysine

smiles: C[N+](C)(C)CCCCC(C(=O)O)N

substrate: trimethyllysine


organism: Bos taurus

substrate_name: alpha-ketoglutarate

substrate: alpha-ketoglutarate


enzyme_display_name: alkyl sulfatase

organism: Pseudomonas putida

substrate_name: nonyl sulfate

proof_lines:
pH 7, 30°C
Raw SKiD Env: Temp=30°C, pH=7

enzyme: alkyl sulfatase

substrate: nonyl sulfate


enzyme_display_name: flavanone 3-dioxygenase

organism: x Ascocenda

substrate_name: naringenin

enzyme: flavanone 3-dioxygenase

substrate: naringenin


organism: Albuca bracteata


enzyme_display_name: methylphosphonate hydroxylase

organism: Gimesia maris

substrate_name: methylphosphonate

enzyme: methylphosphonate hydroxylase

substrate: methylphosphonate


enzyme_display_name: terephthalate 1,2-dioxygenase

organism: Comamonas sp

substrate_name: NADP (at 30 degrees Celsius and at ph 7)

assay_conditions_summary: pH 7; cofactor NADPH

enzyme: terephthalate 1,2-dioxygenase


enzyme_display_name: pyrrole-2-carboxylate monooxygenase

organism: Rhodococcus sp. (in: high G+C Gram-positive bacteria)

substrate_name: NADH

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADH

enzyme: pyrrole-2-carboxylate monooxygenase

substrate: NADH


enzyme_display_name: 2-aminobenzenesulfonate 2,3-dioxygenase

organism: Alcaligenes sp

enzyme: 2-aminobenzenesulfonate 2,3-dioxygenase


organism: Hyphomicrobium sulfonivorans

substrate_name: Dimethyl sulfide

smiles: CSC

substrate: Dimethyl sulfide


enzyme_display_name: erythromycin 12 hydroxylase

organism: Saccharopolyspora erythraea

substrate_name: erythromycin D

enzyme: erythromycin 12 hydroxylase

substrate: erythromycin D


enzyme_display_name: L-ornithine N(5)-monooxygenase (NADPH)

organism: Aspergillus fumigatus

substrate_name: L-Ornithine

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

enzyme: L-ornithine N(5)-monooxygenase (NADPH)

substrate: L-Ornithine


substrate_name: NADPH

assay_conditions_summary: pH 7.6; 298.15 K; cofactor NADPH

substrate: NADPH


substrate_name: L-ornithine

substrate: L-ornithine


enzyme_display_name: 4-hydroxybenzoate 3-monooxygenase

organism: Pseudomonas aeruginosa

assay_conditions_summary: pH 6.8; 277.15 K; cofactor NADPH

enzyme: 4-hydroxybenzoate 3-monooxygenase


organism: Moraxella sp.

assay_conditions_summary: cofactor NADH


enzyme_display_name: cyclohexanone monooxygenase

organism: Acinetobacter sp.

assay_conditions_summary: pH 7.7; 298.15 K; cofactor NADPH

enzyme: cyclohexanone monooxygenase


substrate_name: cyclohexanone

substrate: cyclohexanone


organism: Ilyonectria destructans

substrate_name: Cyclopentanone

substrate: Cyclopentanone


organism: Rhodococcus sp. HI-31

proof_lines:
Raw SKiD Env: Temp=-----, pH=7.5
Raw SKiD Env: Temp=25°C, pH=9


enzyme_display_name: F-actin monooxygenase

organism: Mus musculus

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

assay_conditions_summary: pH 7; 298.15 K; cofactor NADPH

enzyme: F-actin monooxygenase


enzyme_display_name: toluene 4-monooxygenase

organism: Pseudomonas mendocina

substrate_name: Benzene

proof_lines:
Raw SKiD Env: Temp=4°C, pH=7.5
Raw SKiD Env: Temp=25°C, pH=7.5
Raw SKiD Env: Temp=25°C, pH=-----

enzyme: toluene 4-monooxygenase

substrate: Benzene


substrate_name: Toluene

substrate: Toluene


enzyme_display_name: 3-hydroxybenzoate 6-monooxygenase

organism: Pseudomonas alcaligenes

substrate_name: H+; 3-Hydroxybenzoate; NADH; O2

enzyme: 3-hydroxybenzoate 6-monooxygenase

substrate: H+; 3-Hydroxybenzoate; NADH; O2


organism: Polaromonas naphthalenivorans

substrate_name: H+; O2; 3-Hydroxybenzoate; NADH

assay_conditions_summary: cofactor NADPH

substrate: H+; O2; 3-Hydroxybenzoate; NADH


enzyme_display_name: 4-aminobenzoate 1-monooxygenase

organism: Agaricus bisporus

proof_lines:
at pH 7
at pH 8

enzyme: 4-aminobenzoate 1-monooxygenase


organism: Klebsiella oxytoca


enzyme_display_name: L-lysine N(6)-monooxygenase (NADPH)

organism: Escherichia coli

enzyme: L-lysine N(6)-monooxygenase (NADPH)


enzyme_display_name: albendazole monooxygenase

organism: Rattus norvegicus

substrate_name: albendazole

enzyme: albendazole monooxygenase

substrate: albendazole


organism: Nocardia farcinica


enzyme_display_name: flavin-containing monooxygenase

organism: Bos taurus

substrate_name: Methimazole

enzyme: flavin-containing monooxygenase

substrate: Methimazole


enzyme_display_name: 4-hydroxybenzoate 1-hydroxylase

organism: Candida parapsilosis

assay_conditions_summary: pH 7.5; 303.15 K; cofactor FAD

enzyme: 4-hydroxybenzoate 1-hydroxylase


enzyme_display_name: phenol 2-monooxygenase (NADPH)

organism: Corynebacterium glutamicum

substrate_name: phenol

assay_conditions_summary: pH 8; 318.15 K; cofactor NADPH

enzyme: phenol 2-monooxygenase (NADPH)

substrate: phenol


substrate_name: trimethylamine

substrate: trimethylamine


organism: Homo sapiens

substrate_name: sulindac sulfide

substrate: sulindac sulfide


sequence_source: UniProt:P31513 full-length canonical sequence checked, but not accepted for this kinetic tuple because Table 4 contains full-length and C-terminal-truncated FMO3 rows.


enzyme_display_name: unspecific monooxygenase

organism: Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711)

substrate_name: palmitic acid

enzyme: unspecific monooxygenase

substrate: palmitic acid


organism: Bacillus subtilis

substrate_name: NADH

assay_conditions_summary: pH 7.4; 303.15 K; cofactor NADPH

substrate: NADH


organism: Fusarium oxysporum


substrate_name: NADPH; O2; H+; Dodecanoate

substrate: NADPH; O2; H+; Dodecanoate


substrate_name: all-trans-retinoate

substrate: all-trans-retinoate


substrate_name: all-trans retinol

substrate: all-trans retinol


substrate_name: Arachidonate; O2; NADPH; H+

substrate: Arachidonate; O2; NADPH; H+


substrate_name: 17beta-estradiol (2-hydroxylation)

substrate: 17beta-estradiol (2-hydroxylation)


substrate_name: Phenacetin

substrate: Phenacetin


substrate_name: Indole

substrate: Indole


substrate_name: Melatonin

substrate: Melatonin


substrate_name: Coumarin

substrate: Coumarin


substrate_name: O2; H+; Deltamethrin; NADPH

substrate: O2; H+; Deltamethrin; NADPH


enzyme_display_name: 4-hydroxyacetophenone monooxygenase

organism: Pseudomonas fluorescens

substrate_name: NADPH (at pH 7.5)

enzyme: 4-hydroxyacetophenone monooxygenase

substrate: NADPH (at pH 7.5)


substrate_name: O2; NADPH; Fenthion; H+

substrate: O2; NADPH; Fenthion; H+


substrate_name: O2; H+; NADPH; Fenthion

substrate: O2; H+; NADPH; Fenthion


substrate_name: H+; NADPH; Fenthion; O2

substrate: H+; NADPH; Fenthion; O2


substrate_name: Cyclophosphamide

substrate: Cyclophosphamide


substrate_name: Fimasartan

substrate: Fimasartan


organism: Mus musculus

substrate_name: O2; Retinoate; H+; NADPH

substrate: O2; Retinoate; H+; NADPH


substrate_name: dodecanoic acid

smiles: CCCCCCCCCCCC(=O)O

substrate: dodecanoic acid


organism: Phanerodontia chrysosporium

substrate_name: nonanol

smiles: CCCCCCCCCO

substrate: nonanol


substrate_name: (5Z,8Z,11Z,14Z)-eicosatetraenoate (total epoxygenase activity)


substrate_name: Deltamethrin

substrate: Deltamethrin


enzyme_display_name: N-methylcoclaurine 3'-monooxygenase

organism: Eschscholzia californica

substrate_name: H+; NADPH; O2; (S)-N-Methylcoclaurine

enzyme: N-methylcoclaurine 3'-monooxygenase

substrate: H+; NADPH; O2; (S)-N-Methylcoclaurine


enzyme_display_name: vinorine hydroxylase

organism: Rauvolfia serpentina

substrate_name: vinorine

enzyme: vinorine hydroxylase

substrate: vinorine


enzyme_display_name: tabersonine 16-hydroxylase

organism: Catharanthus roseus

substrate_name: H+; NADPH; O2; Tabersonine

enzyme: tabersonine 16-hydroxylase

substrate: H+; NADPH; O2; Tabersonine


enzyme_display_name: taxane 13alpha-hydroxylase

organism: Taxus cuspidata

enzyme: taxane 13alpha-hydroxylase


enzyme_display_name: oryzalexin E synthase

organism: Oryza sativa

substrate_name: H+; NADPH; O2; ent-Sandaracopimaradiene

enzyme: oryzalexin E synthase

substrate: H+; NADPH; O2; ent-Sandaracopimaradiene


enzyme_display_name: aromatase

substrate_name: NADPH; H+; Testosterone; O2

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S312C to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aromatase(Enzyme) mutant S312C

enzyme: aromatase

substrate: NADPH; H+; Testosterone; O2


substrate_name: H+; Androst-4-ene-3,17-dione; NADPH; O2

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature D309A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aromatase(Enzyme) mutant D309A

substrate: H+; Androst-4-ene-3,17-dione; NADPH; O2


substrate_name: Testosterone; NADPH; H+; O2

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature H128Q to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aromatase(Enzyme) mutant H128Q

substrate: Testosterone; NADPH; H+; O2


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature D309N to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aromatase(Enzyme) mutant D309N


substrate_name: O2; Testosterone; H+; NADPH

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature H128A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aromatase(Enzyme) mutant H128A

substrate: O2; Testosterone; H+; NADPH


substrate_name: O2; H+; Androst-4-ene-3,17-dione; NADPH

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R264C to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aromatase(Enzyme) mutant R264C His-tagged

substrate: O2; H+; Androst-4-ene-3,17-dione; NADPH


substrate_name: H+; O2; NADPH; Testosterone

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature C299A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aromatase(Enzyme) mutant C299A

substrate: H+; O2; NADPH; Testosterone


substrate_name: O2; H+; NADPH; Androst-4-ene-3,17-dione

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R264H to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aromatase(Enzyme) mutant R264H His-tagged

substrate: O2; H+; NADPH; Androst-4-ene-3,17-dione


enzyme_display_name: psoralen synthase

organism: Ammi majus

substrate_name: Marmesin

enzyme: psoralen synthase

substrate: Marmesin


substrate_name: marmesin

substrate: marmesin


enzyme_display_name: abieta-7,13-dien-18-ol hydroxylase

organism: Pinus taeda

substrate_name: H+; NADPH; O2; Abietadienol

enzyme: abieta-7,13-dien-18-ol hydroxylase

substrate: H+; NADPH; O2; Abietadienol


organism: Pastinaca sativa


organism: Kitagawia praeruptora

substrate_name: (+)-marmesin

substrate: (+)-marmesin


substrate_name: abietadienol

substrate: abietadienol


organism: Apium graveolens


enzyme_display_name: steroid 21-monooxygenase

organism: Bos taurus

enzyme: steroid 21-monooxygenase


organism: Sus scrofa


enzyme_display_name: squalene monooxygenase

organism: Candida albicans

substrate_name: squalene

enzyme: squalene monooxygenase

substrate: squalene


organism: Candida parapsilosis


organism: Saccharomyces cerevisiae


enzyme_display_name: taxadiene 5alpha-hydroxylase

substrate_name: taxa-4(5),11(12)-diene (for the native enzyme)

enzyme: taxadiene 5alpha-hydroxylase


enzyme_display_name: steroid 22S-hydroxylase

organism: Arabidopsis thaliana

substrate_name: campesterol

enzyme: steroid 22S-hydroxylase

substrate: campesterol


enzyme_display_name: heme oxygenase (biliverdin-producing)

substrate_name: Heme; NADPH; O2

sequence_source: UniProtKB reviewed accession Q5E9F2, checked via https://rest.uniprot.org/uniprotkb/Q5E9F2.json

enzyme: heme oxygenase (biliverdin-producing)

substrate: Heme; NADPH; O2


enzyme_display_name: steroid 17alpha-monooxygenase

organism: Danio rerio

substrate_name: progesterone

enzyme: steroid 17alpha-monooxygenase

substrate: progesterone


organism: Rattus norvegicus

substrate_name: Hematin; O2; NADPH

substrate: Hematin; O2; NADPH


organism: Mus musculus

substrate_name: progesterone (17-alpha hydroxylation)

substrate: progesterone (17-alpha hydroxylation)


assay_conditions_summary: pH 7.4; 310.15 K; cofactor NADPH


enzyme_display_name: 17alpha-hydroxyprogesterone deacetylase

organism: Homo sapiens

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature N202S to reconstruct the assayed variant sequence.

enzyme: 17alpha-hydroxyprogesterone deacetylase


sequence_source: source_record; UniProt P27786 direct check


organism: Sorghum bicolor

substrate_name: p-hydroxyphenylacetaldoxime

substrate: p-hydroxyphenylacetaldoxime


enzyme_display_name: valine N-monooxygenase

organism: Lotus japonicus

substrate_name: L-valine

smiles: CC(C)C(C(=O)O)N

enzyme: valine N-monooxygenase

substrate: L-valine


organism: Manihot esculenta

substrate_name: H+; O2; NADPH; L-Valine

assay_conditions_summary: pH 7.9; 303.15 K; cofactor NADP+

substrate: H+; O2; NADPH; L-Valine


enzyme_display_name: isoleucine N-monooxygenase

substrate_name: L-isoleucine

smiles: CCC(C)C(C(=O)O)N

enzyme: isoleucine N-monooxygenase

substrate: L-isoleucine


enzyme_display_name: phenylalanine N-monooxygenase

substrate_name: phenylalanine

enzyme: phenylalanine N-monooxygenase

substrate: phenylalanine


enzyme_display_name: (E)-2-methylbutanal oxime monooxygenase

substrate_name: (E)-2-methylbutanal oxime

enzyme: (E)-2-methylbutanal oxime monooxygenase

substrate: (E)-2-methylbutanal oxime


enzyme_display_name: (R)-limonene 6-monooxygenase

enzyme: (R)-limonene 6-monooxygenase


enzyme_display_name: (S)-limonene 7-monooxygenase

smiles: CCC=CCC=CCC=CCC=CCC=CCCCC(=O)O

enzyme: (S)-limonene 7-monooxygenase


enzyme_display_name: quinine 3-monooxygenase

substrate_name: Quinine

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature P467S to reconstruct the assayed variant sequence.

proof_lines: PMCID PMC6555127 Table 2 row CYP3A4*19 (P467S).

enzyme: quinine 3-monooxygenase

substrate: Quinine


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature M445T to reconstruct the assayed variant sequence.

proof_lines: PMCID PMC6555127 Table 2 row CYP3A4*3 (M445T).


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature Q200H to reconstruct the assayed variant sequence.

proof_lines: PMCID PMC6555127 Table 2 row CYP3A4*24 (Q200H).


sequence_variant_note: Resolved canonical sequence from Untrusted worker-applied substitution from canonical UniProt P08684; no trusted row-specific CYP3A4*17 sequence preserved. and applied mutation signature F189S to reconstruct the assayed variant sequence.

proof_lines: PMCID PMC6555127 Table 2 row CYP3A4*17 (F189S).


sequence_variant_note: Resolved canonical sequence from UniProt REST P08684 canonical sequence with worker-applied substitution from selected BRENDA/PMID variant label and applied mutation signature I427V to reconstruct the assayed variant sequence.

proof_lines: PMCID PMC6555127 Table 2 row CYP3A4*34 (I427V).


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature T363M to reconstruct the assayed variant sequence.

proof_lines: PMCID PMC6555127 Table 2 row CYP3A4*11 (T363M).


enzyme_display_name: 1,8-cineole 2-exo-monooxygenase

substrate_name: 17beta-estradiol (2-hydroxylation)

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

enzyme: 1,8-cineole 2-exo-monooxygenase

substrate: 17beta-estradiol (2-hydroxylation)


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature L293P to reconstruct the assayed variant sequence.

proof_lines: PMCID PMC6555127 Table 2 row CYP3A4*18 (L293P).


substrate_name: taurochenodeoxycholate

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

substrate: taurochenodeoxycholate


sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


enzyme_display_name: trimethyltridecatetraene synthase

substrate_name: (E,E)-geranyllinalool

enzyme: trimethyltridecatetraene synthase

substrate: (E,E)-geranyllinalool


organism: Zea mays

substrate_name: NADPH; O2; (6E,10E)-Geranyllinalool; H+

substrate: NADPH; O2; (6E,10E)-Geranyllinalool; H+


substrate_name: (E)-nerolidol

substrate: (E)-nerolidol


enzyme_display_name: dimethylnonatriene synthase

enzyme: dimethylnonatriene synthase


enzyme_display_name: syn-pimaradiene 3-monooxygenase

organism: Oryza sativa

enzyme: syn-pimaradiene 3-monooxygenase


enzyme_display_name: long-chain fatty acid omega-monooxygenase

organism: Starmerella bombicola

substrate_name: oleic acid

substrate: oleic acid


enzyme_display_name: flavonoid 3',5'-hydroxylase

organism: Billardiera heterophylla

substrate_name: dihydrokaempferol

enzyme: flavonoid 3',5'-hydroxylase

substrate: dihydrokaempferol


substrate_name: kaempferol

substrate: kaempferol


substrate_name: naringenin

substrate: naringenin


enzyme_display_name: flavonoid 3'-monooxygenase

organism: Perilla frutescens

enzyme: flavonoid 3'-monooxygenase


organism: Fragaria vesca


enzyme_display_name: geraniol 8-hydroxylase

organism: Catharanthus roseus

substrate_name: nerol

enzyme: geraniol 8-hydroxylase

substrate: nerol


enzyme_display_name: pentalenic acid synthase

organism: Streptomyces avermitilis

substrate_name: Compactin

proof_lines:
pH 7.4, 30°C
pH 8.0, 25°C
Raw SKiD Env: Temp=30°C, pH=7.4

enzyme: pentalenic acid synthase

substrate: Compactin


enzyme_display_name: cholestanetriol 26-monooxygenase

substrate_name: O2; 3alpha,7alpha,12alpha-Trihydroxy-5beta-cholestane; H+; NADPH

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

enzyme: cholestanetriol 26-monooxygenase


enzyme_display_name: vitamin D3 24-hydroxylase

organism: Gallus gallus

substrate_name: Calcidiol; NADPH; H+; O2

km_unit: µM

sequence_source: Selected-batch canonical sequence from UniProt REST O73684

enzyme: vitamin D3 24-hydroxylase

substrate: Calcidiol; NADPH; H+; O2


enzyme_display_name: calcidiol 1-monooxygenase

substrate_name: 25-hydroxyvitamin D3

enzyme: calcidiol 1-monooxygenase

substrate: 25-hydroxyvitamin D3


substrate_name: O2; Calcidiol; H+; NADPH

substrate: O2; Calcidiol; H+; NADPH


enzyme_display_name: zeaxanthin epoxidase

organism: Spinacia oleracea

substrate_name: NADH; H+; O2; Zeaxanthin

enzyme: zeaxanthin epoxidase

substrate: NADH; H+; O2; Zeaxanthin


organism: Sus scrofa

substrate_name: H+; Calcidiol; O2; NADPH

substrate: H+; Calcidiol; O2; NADPH


organism: Mycolicibacterium smegmatis


enzyme_display_name: alkane 1-monooxygenase

organism: Ectopseudomonas oleovorans

substrate_name: laurate

enzyme: alkane 1-monooxygenase

substrate: laurate


organism: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)


enzyme_display_name: corticosterone 18-monooxygenase

organism: Homo sapiens

substrate_name: cortisol

enzyme: corticosterone 18-monooxygenase

substrate: cortisol


enzyme_display_name: steroid 15beta-monooxygenase

organism: Priestia megaterium

substrate_name: deoxycorticosterone

enzyme: steroid 15beta-monooxygenase

substrate: deoxycorticosterone


enzyme_display_name: phenylalanine 4-monooxygenase

organism: Chromobacterium violaceum

substrate_name: L-Phenylalanine

sequence_source: UniProtKB P30967 reviewed-entry check on 2026-08-25; canonical accession context only, not a validated S230P variant sequence.

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

enzyme: phenylalanine 4-monooxygenase

substrate: L-Phenylalanine


substrate_name: L-cyclohexylalanine

substrate: L-cyclohexylalanine


organism: Caenorhabditis elegans

substrate_name: tetrahydrobiopterin

sequence_source: UniProtKB REST Q9XYQ5 -> primary P90925 (checked 2026-06-30)

assay_conditions_summary: pH 4

substrate: tetrahydrobiopterin


enzyme_display_name: tyrosine 3-monooxygenase

enzyme: tyrosine 3-monooxygenase


organism: Rattus norvegicus


enzyme_display_name: tryptophan 5-monooxygenase

organism: Dictyostelium discoideum

substrate_name: L-Phe with BH(4) as cofactor

enzyme: tryptophan 5-monooxygenase

substrate: L-Phe with BH(4) as cofactor


substrate_name: L-phenylalanine (at 25 degrees Celsius)

substrate: L-phenylalanine (at 25 degrees Celsius)


enzyme_display_name: dopamine beta-monooxygenase

substrate_name: ascorbate

enzyme: dopamine beta-monooxygenase

substrate: ascorbate


enzyme_display_name: aminocyclopropanecarboxylate oxidase

organism: Malus domestica

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S246A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant S246A

proof_lines: recombinant His-tagged mutant S246A, pH 7.2, 20°C

enzyme: aminocyclopropanecarboxylate oxidase


organism: Diospyros kaki


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature E109M to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant E109M


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S257A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant S257A


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature V159G to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant V159G


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S246F to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant S246F


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S246G to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant S246G


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S246Y to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant S246Y


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature T157A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant T157A

proof_lines: recombinant His-tagged mutant T157A, pH 7.2, 20°C


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R244K to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant R244K

proof_lines: recombinant His-tagged mutant R244K, pH 7.2, 20°C


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R244A to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant R244A


sequence_variant_note: Resolved canonical sequence from Not accepted for this variant: the supplied provenance is a reconstruction from canonical UniProtKB Q00985, not trusted R244G row-specific sequence evidence. and applied mutation signature R244G to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant R244G


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature R175Q to reconstruct the assayed variant sequence.

proof_lines: recombinant His-tagged mutant R175Q, pH 7.2, 20°C


enzyme_display_name: tyrosinase

organism: Bos taurus

substrate_name: L-Adrenaline; O2

sequence_source: UniProtKB exact accession Q8MIU0 REST lookup

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

enzyme: tyrosinase

substrate: L-Adrenaline; O2


substrate_name: O2; L-Adrenaline

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

substrate: O2; L-Adrenaline


sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature S246T to reconstruct the assayed variant sequence.

proof_lines: SABIO-RK modifier species: aminocyclopropanecarboxylate oxidase(Enzyme) mutant S246T


organism: Euterpe edulis

substrate_name: chlorogenic acid

substrate: chlorogenic acid


organism: Camellia sinensis

substrate_name: catechol

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

substrate: catechol


organism: Neurospora crassa

substrate_name: L-Dopa

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

substrate: L-Dopa


organism: Euterpe oleracea

sequence_source: UniProtKB direct accession lookup: Q3YJ63


organism: Priestia aryabhattai

substrate_name: L-tyrosine

substrate: L-tyrosine


organism: Triticum aestivum

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


organism: Carthamus tinctorius


enzyme_display_name: sphingolipid 4-desaturase

organism: Gallus gallus

substrate_name: all-trans-retinol

enzyme: sphingolipid 4-desaturase

substrate: all-trans-retinol


organism: Pholiota microspora

substrate_name: L-dopa

substrate: L-dopa


enzyme_display_name: acyl-CoA 6-desaturase

substrate_name: linoleoyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: acyl-CoA 6-desaturase

substrate: linoleoyl-CoA


enzyme_display_name: (S)-nandinine synthase

organism: Eschscholzia californica

substrate_name: H+; NADPH; O2; (R,S)-Scoulerine

enzyme: (S)-nandinine synthase

substrate: H+; NADPH; O2; (R,S)-Scoulerine


enzyme_display_name: acyl-lipid omega-3 desaturase (cytochrome b5)

organism: Mortierella alpina

substrate_name: alpha-linolenoyl-CoA

sequence_source: UniProtKB U5Y6A8 (canonical sequence available; direct accession lookup)

assay_conditions_summary: pH 7.5; 301.15 K; cofactor CoA

substrate: alpha-linolenoyl-CoA


enzyme_display_name: flavone synthase II

organism: Glycine max

substrate_name: liquiritigenin

enzyme: flavone synthase II

substrate: liquiritigenin


substrate_name: eriodictyol

substrate: eriodictyol


organism: Oryza sativa subsp. japonica

substrate_name: naringenin

substrate: naringenin


organism: Dictyostelium discoideum

substrate_name: L-Phe with BH(4) as cofactor

substrate: L-Phe with BH(4) as cofactor


enzyme_display_name: spheroidene monooxygenase

organism: Rubrivivax gelatinosus

substrate_name: spheroidene

enzyme: spheroidene monooxygenase

substrate: spheroidene


enzyme_display_name: 6beta-hydroxyhyoscyamine epoxidase

organism: Hyoscyamus niger

enzyme: 6beta-hydroxyhyoscyamine epoxidase


organism: Xenorhabdus nematophila


enzyme_display_name: anthocyanidin synthase

organism: Perilla frutescens

enzyme: anthocyanidin synthase


enzyme_display_name: flavone synthase I

organism: Plagiochasma appendiculatum

enzyme: flavone synthase I


enzyme_display_name: flavonol synthase

organism: Camellia sinensis

substrate_name: hesperetin

sequence_source: UniProtKB:A0A3G1RNB1 direct exact-accession lookup

enzyme: flavonol synthase

substrate: hesperetin


organism: Crocosmia x crocosmiiflora

sequence_source: Direct UniProtKB JSON/FASTA lookup for A0A4D6Q440


organism: Pseudomonas syringae


enzyme_display_name: prostaglandin-endoperoxide synthase

organism: Mus musculus

substrate_name: arachidonate

enzyme: prostaglandin-endoperoxide synthase

substrate: arachidonate


enzyme_display_name: tyrosine isonitrile desaturase

organism: Pseudomonas aeruginosa

enzyme: tyrosine isonitrile desaturase


organism: Ovis aries

smiles: CCCCCC(C=CC=CCC=CCC=CCCCC(=O)O)OO


smiles: OO


enzyme_display_name: Deleted entry

organism: Rattus norvegicus

substrate_name: H+; NADPH; Aquacob(III)alamin

enzyme: Deleted entry

substrate: H+; NADPH; Aquacob(III)alamin


organism: Euglena gracilis

substrate_name: H+; NADPH; Hydroxycob(lll)alamin

substrate: H+; NADPH; Hydroxycob(lll)alamin


enzyme_display_name: ferric-chelate reductase (NADH)

organism: Solanum lycopersicum

substrate_name: Fe3+; NADH; H+

enzyme: ferric-chelate reductase (NADH)

substrate: Fe3+; NADH; H+


organism: Paracoccus denitrificans

substrate_name: NADH; Fe3+; H+

substrate: NADH; Fe3+; H+


enzyme_display_name: ferric-chelate reductase (NADPH)

organism: Homo sapiens

substrate_name: NADPH; Fe3+

sequence_source: UniProtKB P30043 canonical entry (accession-backed compact identity evidence)

enzyme: ferric-chelate reductase (NADPH)

substrate: NADPH; Fe3+


enzyme_display_name: xanthine dehydrogenase

organism: Capra hircus

substrate_name: xanthine

assay_conditions_summary: pH 7.2; 298.15 K; cofactor NAD+

enzyme: xanthine dehydrogenase

substrate: xanthine


enzyme_display_name: formate dehydrogenase (NADP(+))

organism: Neomoorella thermoacetica

substrate_name: formate (at 55 degrees Celsius and pH 7.5, with NADP as electron acceptor)

enzyme: formate dehydrogenase (NADP(+))


substrate_name: NAD+

substrate: NAD+


organism: Rhodobacter capsulatus

substrate_name: Xanthine

substrate: Xanthine


enzyme_display_name: nicotinate dehydrogenase

organism: Bacillus sp. (in: firmicutes)

substrate_name: nicotinate

enzyme: nicotinate dehydrogenase

substrate: nicotinate


organism: Escherichia coli (strain K12)

substrate_name: NADH

substrate: NADH


enzyme_display_name: formate dehydrogenase

organism: Methylorubrum extorquens

substrate_name: formate

assay_conditions_summary: pH 7; 303.15 K; cofactor NAD+

enzyme: formate dehydrogenase

substrate: formate


organism: Bacillus subtilis

substrate_name: dihydrodipicolinate

substrate: dihydrodipicolinate


organism: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

substrate_name: NADH (at 30 degrees Celsius)

substrate: NADH (at 30 degrees Celsius)


organism: Lotus japonicus

assay_conditions_summary: pH 7.5; 303.15 K; buffer 0.1 M potassium phosphate; cofactor NADP+

proof_lines: in 0.1 M potassium phosphate buffer, pH 7.5, at 30°C


organism: Thiobacillus sp.

assay_conditions_summary: pH 7; 298.15 K; cofactor NAD+

proof_lines:
at pH 7.0 and 25°C
pH 7.0, 30°C


organism: Thermochaetoides thermophila


organism: Moraxella sp.

assay_conditions_summary: cofactor NAD+


organism: Ogataea angusta

sequence_source: UniProtKB:P33677 direct accession lookup

assay_conditions_summary: pH 6.5; 303.15 K; cofactor NAD+


enzyme_display_name: formate dehydrogenase (cytochrome c-553)

organism: Nitratidesulfovibrio vulgaris


enzyme_display_name: lupanine 17-hydroxylase (cytochrome c)

organism: Pseudomonas sp.

substrate_name: lupanine

enzyme: lupanine 17-hydroxylase (cytochrome c)

substrate: lupanine


enzyme_display_name: ferredoxin--NADP(+) reductase

organism: Chlorobaculum tepidum

substrate_name: NADPH

assay_conditions_summary: pH 7; 298.15 K; buffer 2 mM ferricyanide in 20 mM HEPES-NaOH; cofactor NADP+

proof_lines: with 2 mM ferricyanide in 20 mM HEPES-NaOH buffer, at pH 7.0 and 25°C

enzyme: ferredoxin--NADP(+) reductase

substrate: NADPH


organism: Hydrogenobacter thermophilus

substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

substrate: NADP+


enzyme_display_name: limonene dehydrogenase

organism: Castellaniella defragrans (strain DSM 12143 / CCUG 39792 / 65Phen)

substrate_name: limonene (at 28 degrees Celsius)

enzyme: limonene dehydrogenase

substrate: limonene (at 28 degrees Celsius)


enzyme_display_name: adrenodoxin-NADP(+) reductase

organism: Arabidopsis thaliana

assay_conditions_summary: cofactor NADPH

enzyme: adrenodoxin-NADP(+) reductase


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)


enzyme_display_name: alcohol dehydrogenase

organism: Drosophila simulans

enzyme: alcohol dehydrogenase


organism: Drosophila virilis


organism: Homo sapiens

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

smiles: CCCCCCO


substrate_name: Ethanol

smiles: CCO

substrate: Ethanol


organism: Geobacillus stearothermophilus

sequence_source: UniProt REST direct accession lookup: P42328

smiles: C(CO)CO


substrate_name: Retinol

substrate: Retinol


substrate_name: benzyl alcohol

substrate: benzyl alcohol


smiles: CCCCCCCCO


substrate_name: methanol

smiles: CO

substrate: methanol


smiles: CCCCCO


assay_conditions_summary: pH 9; 298.15 K; cofactor NAD+


substrate_name: 12-Hydroxydodecanoic acid

smiles: O=C(O)CCCCCCCCCCCO

substrate: 12-Hydroxydodecanoic acid


organism: Kluyveromyces lactis

assay_conditions_summary: pH 8; 298.15 K; cofactor NADH


assay_conditions_summary: pH 8; 298.15 K; cofactor NADPH


assay_conditions_summary: pH 8; 298.15 K; cofactor NADP+


assay_conditions_summary: pH 8; 298.15 K; cofactor NAD+


substrate_name: Acetaldehyde

smiles: CC=O

substrate: Acetaldehyde


substrate_name: NAD+

substrate: NAD+


organism: Macaca mulatta

assay_conditions_summary: pH 7.3; 303.15 K; cofactor NAD+


assay_conditions_summary: pH 6; 298.15 K; cofactor NAD+


smiles: CCCCO

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NAD+


smiles: CC(C)O


substrate_name: Acetone

smiles: CC(C)=O

substrate: Acetone


organism: Mus musculus

substrate_name: all-trans-Retinal

substrate: all-trans-Retinal


smiles: CCCO


organism: Escherichia coli

substrate_name: ethyl acetoacetate

smiles: CCOC(=O)CC(=O)C

substrate: ethyl acetoacetate


organism: Mycobacterium tuberculosis

assay_conditions_summary: pH 7.6; 303.15 K; cofactor NADPH


organism: Persea americana

substrate_name: acetoacetyl-CoA

substrate: acetoacetyl-CoA


organism: Saccharomyces cerevisiae

substrate_name: ethanol

assay_conditions_summary: pH 7

substrate: ethanol


enzyme_display_name: carbonyl reductase (NADPH)

organism: Candida magnoliae

substrate_name: Ethyl 4-chloro-3-oxobutanoate; NADPH; H+

enzyme: carbonyl reductase (NADPH)


substrate_name: S-Nitrosoglutathione; NADPH; H+

substrate: S-Nitrosoglutathione; NADPH; H+


substrate_name: 9,10-Phenanthroquinone; H+; NADPH

substrate: 9,10-Phenanthroquinone; H+; NADPH


substrate_name: H+; Menadione; NADPH

substrate: H+; Menadione; NADPH


substrate_name: Menadione; NADPH; H+

substrate: Menadione; NADPH; H+


organism: Mesocricetus auratus

substrate_name: Acetone; NADH; H+

assay_conditions_summary: cofactor NADH

substrate: Acetone; NADH; H+


enzyme_display_name: alcohol dehydrogenase (NADP+)

organism: Cavia porcellus

enzyme: alcohol dehydrogenase (NADP+)


organism: Gallus gallus

substrate_name: H+; NADPH; 4-Pyridinecarboxaldehyde

substrate: H+; NADPH; 4-Pyridinecarboxaldehyde


substrate_name: NADPH; Acetone

substrate: NADPH; Acetone


organism: Bos taurus

substrate_name: 4-Carboxybenzaldehyde; NADPH

substrate: 4-Carboxybenzaldehyde; NADPH


organism: Rattus norvegicus

substrate_name: NADPH; 4-Nitroacetophenone

substrate: NADPH; 4-Nitroacetophenone


organism: Marinobacter nauticus VT8

smiles: CCCCC=CCCCCCCCCCC=O


substrate_name: NADPH; Benzaldehyde

substrate: NADPH; Benzaldehyde


substrate_name: NADPH; H+; Glyceraldehyde

substrate: NADPH; H+; Glyceraldehyde


organism: Pyrococcus furiosus

substrate_name: NADP+

assay_conditions_summary: pH 10; 343.15 K; cofactor NADP+

substrate: NADP+


organism: Mycolicibacterium smegmatis

substrate_name: octanal

smiles: CCCCCCCC=O

substrate: octanal


organism: Sus scrofa

substrate_name: NADP+; 4-Hydroxybutyrate

substrate: NADP+; 4-Hydroxybutyrate


organism: Thermoanaerobacter ethanolicus

substrate_name: NADP+; 2-Butanol

substrate: NADP+; 2-Butanol


organism: Thermococcus sibiricus

substrate_name: D-xylose

assay_conditions_summary: pH 10.5; 333.15 K; cofactor NADP+

substrate: D-xylose


organism: Thermoanaerobacter sp.

substrate_name: NADPH

substrate: NADPH


enzyme_display_name: aldose reductase

organism: Candida tenuis

substrate_name: D-Xylose

assay_conditions_summary: pH 7; 298.15 K; cofactor NADPH

enzyme: aldose reductase

substrate: D-Xylose


substrate_name: NADH

assay_conditions_summary: pH 7; 298.15 K; cofactor NADH

substrate: NADH


organism: Homo sapiens

substrate_name: Acetoacetyl-CoA; H+; NADPH

substrate: Acetoacetyl-CoA; H+; NADPH


organism: Sinomonas cyclohexanicum

assay_conditions_summary: cofactor NAD+


enzyme_display_name: morphine 6-dehydrogenase

organism: Oryctolagus cuniculus

substrate_name: NAD+

enzyme: morphine 6-dehydrogenase

substrate: NAD+


enzyme_display_name: chlordecone reductase

substrate_name: Chlordecone

sequence_source: unresolved accession-backed assignment

enzyme: chlordecone reductase

substrate: Chlordecone


organism: Plasmodium falciparum

substrate_name: acetoacetyl-CoA

assay_conditions_summary: pH 7.5; cofactor NADPH

substrate: acetoacetyl-CoA


organism: Arabidopsis thaliana

substrate_name: farnesol

smiles: CC(=CCCC(=CCCC(=CCO)C)C)C

substrate: farnesol


enzyme_display_name: methylglyoxal reductase (NADPH)

organism: Saccharomyces cerevisiae

assay_conditions_summary: cofactor NADPH

enzyme: methylglyoxal reductase (NADPH)


organism: Kluyveromyces marxianus

substrate_name: methyl glyoxal

sequence_source: UniProtKB A0A0E4AX59 REST lookup on 2026-06-01; accession was source-provided by BRENDA in the selected batch.

smiles: CC(=O)C=O

assay_conditions_summary: pH 6; cofactor NADPH

substrate: methyl glyoxal


organism: Candida boidinii

substrate_name: Reduced glutathione; Formaldehyde; NAD+

substrate: Reduced glutathione; Formaldehyde; NAD+


organism: [Candida] boidinii


organism: Canis lupus familiaris

substrate_name: glutathione

smiles: C(CC(=O)NC(CS)C(=O)NCC(=O)O)C(C(=O)O)N

substrate: glutathione


organism: Drosophila melanogaster

substrate_name: S-hydroxymethyl glutathione

substrate: S-hydroxymethyl glutathione


substrate_name: S-(Hydroxymethyl)glutathione

substrate: S-(Hydroxymethyl)glutathione


organism: Komagataella pastoris

substrate_name: formaldehyde

substrate: formaldehyde


organism: Bos taurus

substrate_name: Formaldehyde; NAD+; Reduced glutathione

sequence_source: UniProt REST direct accession lookup for Q3ZC42 on 2026-06-23

substrate: Formaldehyde; NAD+; Reduced glutathione


organism: Taiwanofungus camphoratus

substrate_name: S-nitrosoglutathione

sequence_source: UniProt D2CFJ2 FASTA checked directly on 2026-06-23

substrate: S-nitrosoglutathione


organism: Deinococcus radiodurans

assay_conditions_summary: pH 7.8; 303.15 K; cofactor NAD+


enzyme_display_name: D-arabinitol dehydrogenase (NADP(+))

organism: Gluconobacter oxydans

substrate_name: glycerol

smiles: C(C(CO)O)O

enzyme: D-arabinitol dehydrogenase (NADP(+))

substrate: glycerol


organism: Thermococcus kodakarensis

substrate_name: homoisocitrate

assay_conditions_summary: pH 8; 333.15 K; cofactor NAD+

substrate: homoisocitrate


enzyme_display_name: D-2-hydroxyacid dehydrogenase (NAD(+))

organism: Lactobacillus delbrueckii

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NAD+

enzyme: D-2-hydroxyacid dehydrogenase (NAD(+))


enzyme_display_name: homoserine dehydrogenase

organism: Thermus thermophilus

substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

enzyme: homoserine dehydrogenase

substrate: NADP+


organism: Halobacterium mediterranei

substrate_name: 2-Oxoisoleucine; NADH; H+

substrate: 2-Oxoisoleucine; NADH; H+


organism: Corynebacterium sp.

substrate_name: 2-Dehydro-L-idonate; NADPH; H+

substrate: 2-Dehydro-L-idonate; NADPH; H+


enzyme_display_name: geraniol dehydrogenase (NAD(+))

organism: Carpoglyphus lactis

substrate_name: geraniol

smiles: CC(=CCCC(=CCO)C)C

enzyme: geraniol dehydrogenase (NAD(+))

substrate: geraniol


enzyme_display_name: D-chiro-inositol 1-dehydrogenase

organism: Thermotoga maritima

enzyme: D-chiro-inositol 1-dehydrogenase


enzyme_display_name: (R,R)-butanediol dehydrogenase

organism: Clostridium autoethanogenum

substrate_name: (R)-acetoin

enzyme: (R,R)-butanediol dehydrogenase

substrate: (R)-acetoin


substrate_name: acetone

substrate: acetone


smiles: CC(C(C)O)O


substrate_name: acetoin

smiles: CC(=O)C(C)O

substrate: acetoin


smiles: CCC(C)=O


substrate_name: NADPH

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

substrate: NADPH


organism: Rhodococcus erythropolis

substrate_name: diacetyl

smiles: CC(=O)C(C)=O

substrate: diacetyl


substrate_name: NAD+

assay_conditions_summary: pH 6.5; 328.15 K; cofactor NAD+

substrate: NAD+


substrate_name: NADH

assay_conditions_summary: pH 10; 318.15 K; cofactor NADH

substrate: NADH


organism: Saccharomyces cerevisiae

smiles: CCC(O)CO


substrate_name: glycolaldehyde

smiles: O=CCO

substrate: glycolaldehyde


substrate_name: acetaldehyde

smiles: CC=O

substrate: acetaldehyde


smiles: CC(O)C(C)O


enzyme_display_name: isocitrate dehydrogenase (NADP(+))

organism: Mus musculus

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADP+

proof_lines:
Raw SKiD Env: Temp=25°C, pH=7.4
Raw SKiD Env: Temp=-----, pH=7.5

enzyme: isocitrate dehydrogenase (NADP(+))


organism: Desulfotalea psychrophila

assay_conditions_summary: 278.15 K; cofactor NADP+

proof_lines:
Raw SKiD Env: Temp=5°C, pH=-----
Raw SKiD Env: Temp=25°C, pH=-----


enzyme_display_name: levoglucosan dehydrogenase

organism: Arthrobacter sp. I-552

substrate_name: levoglucosan

assay_conditions_summary: pH 9; 313.15 K; cofactor NAD+

enzyme: levoglucosan dehydrogenase

substrate: levoglucosan


organism: Trypanosoma cruzi


enzyme_display_name: D-xylose 1-dehydrogenase (NADP(+), D-xylono-1,4-lactone-forming)

organism: Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)

substrate_name: D-xylose

substrate: D-xylose


organism: Sus scrofa

proof_lines:
Raw SKiD Env: Temp=25°C, pH=7.4
Raw SKiD Env: Temp=-----, pH=7.4


enzyme_display_name: D-xylose reductase (NADPH)

organism: Meyerozyma guilliermondii

assay_conditions_summary: pH 7.2; 298.15 K; cofactor NADPH

enzyme: D-xylose reductase (NADPH)


organism: Debaryomyces nepalensis

substrate_name: D-Erythrose

assay_conditions_summary: pH 7; 318.15 K; cofactor NADPH

substrate: D-Erythrose


organism: Comamonas testosteroni


organism: Scheffersomyces shehatae

assay_conditions_summary: pH 6.5; 308.15 K; cofactor NADPH


organism: Thermomyces lanuginosus

substrate_name: D-Xylose

assay_conditions_summary: pH 6.5; 323.15 K; cofactor NADPH

substrate: D-Xylose


substrate_name: NAD+; Androsterone

assay_conditions_summary: pH 5.5; 298.15 K; cofactor NAD+

substrate: NAD+; Androsterone


assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADH


organism: Homo sapiens


substrate_name: Dimethylglyoxal

substrate: Dimethylglyoxal


substrate_name: m-Nitrobenzaldehyde

substrate: m-Nitrobenzaldehyde


substrate_name: Benzene dihydrodiol

substrate: Benzene dihydrodiol


substrate_name: Camphorquinone

substrate: Camphorquinone


substrate_name: chenodeoxycholate

substrate: chenodeoxycholate


sequence_source: uniprot_accession (canonical reference only; not validated as the V54L variant)


enzyme_display_name: 7,8-dihydroneopterin oxygenase

organism: Mycobacterium tuberculosis

proof_lines:
at pH 7.0 and 25°C
Raw SKiD Env: Temp=25°C, pH=7

enzyme: 7,8-dihydroneopterin oxygenase


organism: Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)

assay_conditions_summary: cofactor NADPH

enzyme: D-xylose 1-dehydrogenase (NADP(+), D-xylono-1,4-lactone-forming)


substrate_name: NAD+; Estradiol-17beta

assay_conditions_summary: cofactor CoA

substrate: NAD+; Estradiol-17beta


substrate_name: NAD+; 5beta-Dihydrotestosterone

assay_conditions_summary: cofactor NAD+

substrate: NAD+; 5beta-Dihydrotestosterone


organism: Pseudomonas sp.

assay_conditions_summary: cofactor NADH


enzyme_display_name: cellobiose dehydrogenase (acceptor)

organism: Chaetomium sp.

substrate_name: lactose

enzyme: cellobiose dehydrogenase (acceptor)

substrate: lactose


organism: Digitalis lanata

substrate_name: NAD+

substrate: NAD+


organism: Amesia atrobrunnea

proof_lines:
at pH 5.0 and 30°C
at pH 6.0 and 30°C


organism: Termitomyces clypeatus

substrate_name: cellotriose

substrate: cellotriose


organism: Dichomera saubinetii

substrate_name: maltose

substrate: maltose


organism: Irpex lacteus

substrate_name: cellobiose

substrate: cellobiose


organism: Termitomyces sp. OE 147


enzyme_display_name: phosphite dehydrogenase

organism: Nostoc punctiforme

substrate_name: phosphonate

assay_conditions_summary: pH 7.3; 298.15 K; cofactor NAD+

enzyme: phosphite dehydrogenase

substrate: phosphonate


organism: Cupriavidus metallidurans


organism: Thermothielavioides terrestris

assay_conditions_summary: pH 6; 298.15 K; buffer 50 mM MES


organism: Stachybotrys bisbyi

proof_lines:
at pH 5.5 and 30°C
at pH 5.´5 and 30°C


enzyme_display_name: gluconate 2-dehydrogenase (acceptor)

organism: Klebsiella pneumoniae

substrate_name: D-gluconate

assay_conditions_summary: pH 4

enzyme: gluconate 2-dehydrogenase (acceptor)

substrate: D-gluconate


organism: Stutzerimonas stutzeri

assay_conditions_summary: pH 7.5; 303.15 K; cofactor NADP+

proof_lines:
at pH 7.5 and 30°C
pH 7.2, 30°C
pH 7.2, 40°C


enzyme_display_name: arsenate reductase (thioredoxin)

organism: Bacillus subtilis

substrate_name: arsenate

enzyme: arsenate reductase (thioredoxin)

substrate: arsenate


organism: Thermus thermophilus

sequence_source: UniProt REST Q72HI5 canonical sequence checked 2026-06-04; preserved selected-batch sequence is 150 residues.


assay_conditions_summary: pH 7; 298.15 K; cofactor NAD+


enzyme_display_name: isopenicillin-N synthase

organism: Aspergillus nidulans

enzyme: isopenicillin-N synthase


enzyme_display_name: hercynylcysteine S-oxide synthase

organism: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

substrate_name: hercynine

enzyme: hercynylcysteine S-oxide synthase

substrate: hercynine


enzyme_display_name: reticuline oxidase

organism: Eschscholzia californica

substrate_name: (S)-reticuline

sequence_source: UniProt P30986 PMID-linked direct lookup

assay_conditions_summary: pH 9; 310.15 K; cofactor FAD

enzyme: reticuline oxidase

substrate: (S)-reticuline


enzyme_display_name: iodotyrosine deiodinase

organism: Thermotoga neapolitana

substrate_name: 3-chloro-L-tyrosine; 3-iodo-L-tyrosine; 2-iodophenol; 3-bromo-L-tyrosine

sequence_source: UniProtKB B9K712 direct lookup on 2026-06-09; canonical sequence presence and 186-residue length reconfirmed by direct accession check on 2026-08-26.

proof_lines:
at pH 7.4 and 60°C
at pH 7.4 and 25°C

enzyme: iodotyrosine deiodinase


enzyme_display_name: aureusidin synthase

organism: Antirrhinum majus

substrate_name: isoliquiritigenin

enzyme: aureusidin synthase

substrate: isoliquiritigenin


enzyme_display_name: cannabidiolic acid synthase

organism: Cannabis sativa

substrate_name: cannabigerolate

assay_conditions_summary: pH 5; 303.15 K; buffer 100 mM sodium citrate

proof_lines: in 100 mM sodium citrate buffer (pH 5.0), at 30°C

enzyme: cannabidiolic acid synthase

substrate: cannabigerolate


substrate_name: cannabigerolic acid

sequence_source: source_record; canonical sequence retained in selected batch and not restated in generic review output

substrate: cannabigerolic acid


organism: Sphingobium sp. UG30

sequence_source: UniProtKB:Q937X0 direct FASTA/JSON lookup; canonical sequence is preserved in the selected batch.


enzyme_display_name: thyroxine 5-deiodinase

organism: Homo sapiens

substrate_name: L-thyroxine

enzyme: thyroxine 5-deiodinase

substrate: L-thyroxine


enzyme_display_name: thyroxine 5'-deiodinase

organism: Xenopus laevis

substrate_name: L-thyroxine (outer-ring deiodination activity)

enzyme: thyroxine 5'-deiodinase


organism: Dehalobacter restrictus

substrate_name: tetrachloroethene

smiles: C(=C(Cl)Cl)(Cl)Cl

substrate: tetrachloroethene


organism: Dehalobacter restrictus (strain DSM 9455 / PER-K23)


enzyme_display_name: (+)-pinoresinol reductase

organism: Arabidopsis thaliana

substrate_name: (+)-pinoresinol

enzyme: (+)-pinoresinol reductase

substrate: (+)-pinoresinol


organism: Forsythia intermedia


enzyme_display_name: (+)-lariciresinol reductase

enzyme: (+)-lariciresinol reductase


organism: Saccharomyces cerevisiae

substrate_name: NAD+


enzyme_display_name: aminobutyraldehyde dehydrogenase

organism: Pseudomonas fluorescens

enzyme: aminobutyraldehyde dehydrogenase


enzyme_display_name: glycolaldehyde dehydrogenase

organism: Escherichia coli

substrate_name: H2O; Glycolaldehyde; NAD+

sequence_source: UniProtKB:P25553 canonical sequence, direct REST lookup 2026-06-24

assay_conditions_summary: cofactor NADP+

enzyme: glycolaldehyde dehydrogenase

substrate: H2O; Glycolaldehyde; NAD+


organism: Arabidopsis sp.


organism: Ctenocephalides felis

substrate_name: pentanal

smiles: CCCCC=O

substrate: pentanal


organism: Saccharolobus solfataricus

assay_conditions_summary: pH 6.5; 343.15 K; cofactor NAD+

proof_lines:
pH 6.5, 70°C
pH 6.5, 70°C, cofactor NAD+

substrate: NAD+


organism: Thermococcus profundus


enzyme_display_name: enoyl-[acyl-carrier-protein] reductase (NADPH, Si-specific)

organism: Bacillus subtilis

substrate_name: NADPH

sequence_source: UniProtKB REST canonical sequence for P71079, checked 2026-06-19

substrate: NADPH


enzyme_display_name: monoamine oxidase

organism: Aspergillus niger

substrate_name: n-butylamine

smiles: CCCCN

enzyme: monoamine oxidase

substrate: n-butylamine


enzyme_display_name: benzoyl-CoA reductase

organism: Thauera aromatica

substrate_name: benzoyl-CoA

assay_conditions_summary: cofactor ATP

enzyme: benzoyl-CoA reductase

substrate: benzoyl-CoA


enzyme_display_name: dihydrofolate reductase

organism: Gallus gallus

assay_conditions_summary: pH 7.4; cofactor NADPH

proof_lines:
pH 7.4
with NADPH
pH 5.0

enzyme: dihydrofolate reductase


organism: Bos taurus

assay_conditions_summary: pH 7.6; 298.15 K; cofactor NADPH


organism: Glycine max


organism: Lactobacillus leichmannii


organism: Geobacillus stearothermophilus

assay_conditions_summary: pH 7; 333.15 K; cofactor NADPH


organism: Moritella profunda

assay_conditions_summary: pH 7; 303.15 K; cofactor NADPH


proof_lines:
at pH 7.0 and 25°C
at pH 7.0 and 15°C
at pH 7.0 and 20°C


organism: Mycolicibacterium phlei

assay_conditions_summary: cofactor NADPH


organism: Wuchereria bancrofti


organism: Neisseria gonorrhoeae


organism: Rattus norvegicus


organism: Methylorubrum extorquens


enzyme_display_name: cytokinin dehydrogenase

organism: Oryza sativa subsp. japonica

substrate_name: trans-zeatin

enzyme: cytokinin dehydrogenase

substrate: trans-zeatin


assay_conditions_summary: cofactor FAD


enzyme_display_name: D-proline dehydrogenase

organism: Pyrobaculum islandicum

enzyme: D-proline dehydrogenase


organism: Pyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3)

substrate_name: D-proline

substrate: D-proline


organism: Paenarthrobacter nicotinovorans


organism: Azospirillum sp. B510


enzyme_display_name: leghemoglobin reductase

organism: Phaseolus vulgaris

substrate_name: ferrileghemoglobin

enzyme: leghemoglobin reductase

substrate: ferrileghemoglobin


organism: Vigna unguiculata

substrate_name: H+; NADH; Ferrileghemoglobin

sequence_source: UniProtKB Q9SPB1 direct REST/FASTA accession check on 2026-06-24

assay_conditions_summary: cofactor NADH

substrate: H+; NADH; Ferrileghemoglobin


enzyme_display_name: NAD(P)H oxidase (H2O-forming)

organism: Giardia duodenalis

substrate_name: NADH

assay_conditions_summary: pH 7.8; 295.15 K; cofactor NADPH

enzyme: NAD(P)H oxidase (H2O-forming)

substrate: NADH


organism: Escherichia coli

substrate_name: thio-NAD+

assay_conditions_summary: pH 7.5; 308.15 K; cofactor NADPH

substrate: thio-NAD+


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)


enzyme_display_name: NADH oxidase (H2O2-forming)

organism: Methanocaldococcus jannaschii

assay_conditions_summary: pH 7; 356.15 K; cofactor NADH

enzyme: NADH oxidase (H2O2-forming)


enzyme_display_name: NADH oxidase (H2O-forming)

substrate_name: NADH; O2; H+

sequence_source: UniProtKB P24232 canonical sequence direct lookup

enzyme: NADH oxidase (H2O-forming)

substrate: NADH; O2; H+


organism: Clostridium acetobutylicum

substrate_name: O2; H+; NADH

sequence_source: UniProtKB Q97GC0 canonical sequence direct lookup

substrate: O2; H+; NADH


enzyme_display_name: peroxidase

organism: Burkholderia pseudomallei

enzyme: peroxidase


organism: Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / IAM 19013 / LMG 5710 / NBRC 13948 / NRRL B-527 / VKM B-1787 / 2291 / W)

smiles: O=O


organism: Saccharomyces cerevisiae

substrate_name: NADH; H+; O2

substrate: NADH; H+; O2


organism: Streptococcus mutans

assay_conditions_summary: pH 7; 298.15 K; cofactor NADH


enzyme_display_name: Deleted entry

organism: Enterococcus faecalis

substrate_name: H+; NADH; O2

enzyme: Deleted entry

substrate: H+; NADH; O2


organism: Lactococcus lactis subsp. cremoris


assay_conditions_summary: cofactor NAD+


organism: Thermus thermophilus

substrate_name: FAD; NADH

substrate: FAD; NADH


enzyme_display_name: nitroalkane oxidase

organism: Podospora anserina

enzyme: nitroalkane oxidase


organism: Sus scrofa domestica

substrate_name: 2-Nitropropane; H2O; O2

substrate: 2-Nitropropane; H2O; O2


enzyme_display_name: factor independent urate hydroxylase

organism: Canis lupus familiaris

substrate_name: Urate

enzyme: factor independent urate hydroxylase

substrate: Urate


organism: Arthrobacter globiformis

substrate_name: O2; H2O; Urate

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

sequence_source: UniProtKB:D0VWQ1 direct FASTA/JSON lookup.

substrate: O2; H2O; Urate


organism: Kluyveromyces marxianus