organism: Aphanothece halophytica

substrate_name: S-adenosyl-L-methionine

proof_lines: pH 8.8, 37°C, recombinant wild-type enzyme, with dimethylglycine

proof_lines: pH 8.8, 37°C, recombinant wild-type enzyme, with sarcosine

substrate: S-adenosyl-L-methionine


source_db: brenda

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

protein_accession_database: UniProt

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution

proof_lines: pH 8.8, 37°C, recombinant mutant P171Q, with dimethylglycine

source: brenda


organism: Methanohalophilus portucalensis

substrate_name: sarcosine

mutation_signature: Unknown

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: pH 7.3, 37°C, recombinant enzyme, with sarcosine

proof_lines: pH 7.3, 37°C, recombinant enzyme, with N,N-dimethylglycine

substrate: sarcosine


enzyme_display_name: phytepsin

organism: Cynara cardunculus

enzyme: phytepsin


enzyme_display_name: acetylputrescine deacetylase

organism: Pseudomonas aeruginosa

substrate_name: Acetylcadaverine

enzyme: acetylputrescine deacetylase

substrate: Acetylcadaverine


enzyme_display_name: L-tryptophan decarboxylase

organism: Gelatoporia subvermispora

enzyme: L-tryptophan decarboxylase


proof_lines: recombinant wild-type enzyme, pH 7.5, 37°C


enzyme_display_name: phosphonopyruvate decarboxylase

organism: Bacteroides fragilis

proof_lines: 25°C, pH 7.3, 5 mM MgCl2, 1 mM MnCl2, 0.2 mM thiamine diphosphate

proof_lines: 25°C, pH 7.3, 5 mM MgCl2, 1 mM MnCl2, 0.2 mM thiamine pyrophosphate

enzyme: phosphonopyruvate decarboxylase


organism: Streptomyces viridochromogenes

proof_lines: mutant enzyme S25N, at pH 8.0 (HEPES buffer) and at 30°C


proof_lines: mutant enzyme H111A, at pH 8.0 (HEPES buffer) and at 30°C


proof_lines: wild type enzyme, at pH 8.0 (HEPES buffer) and at 30°C


proof_lines: mutant enzyme D297E, at pH 8.0 (HEPES buffer) and at 30°C


enzyme_display_name: peptidyl-dipeptidase Dcp

organism: Escherichia coli

source_db: oed

enzyme: peptidyl-dipeptidase Dcp

source: oed


enzyme_display_name: Oplophorus-luciferin 2-monooxygenase

organism: Oplophorus gracilirostris

substrate_name: Unknown

km_display: Not reported

ki_unit: mM

proof_lines: pH and temperature not specified in the publication

enzyme: Oplophorus-luciferin 2-monooxygenase

substrate: Unknown


enzyme_display_name: butane monooxygenase (soluble)

organism: Thauera butanivorans

substrate_name: pentane

smiles: CCCCC

enzyme: butane monooxygenase (soluble)

substrate: pentane


substrate_name: propane

proof_lines:
product propan-1-ol, pH 7.2, 25°C
pH 7.2, 25°C
product pentan-2-ol, pH 7.2, 25°C

substrate: propane


smiles: CCC


organism: Mus musculus

substrate_name: 4-Nitrocatechol sulfate; H2O

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

substrate: 4-Nitrocatechol sulfate; H2O


enzyme_display_name: beta-primeverosidase

organism: Camellia sinensis

substrate_name: 2-phenylethyl beta-vicianoside

source_license: CC BY 4.0; CC BY-NC-ND 4.0

proof_lines: recombinant His-tagged enzyme, pH and temperature not specified in the publication

source_databases_merged: brenda skid

enzyme: beta-primeverosidase

substrate: 2-phenylethyl beta-vicianoside


enzyme_display_name: DNA-3-methyladenine glycosylase II

organism: Bacillus subtilis

substrate_name: 1,N6-ethenoadenine containing DNA; H2O

enzyme: DNA-3-methyladenine glycosylase II

substrate: 1,N6-ethenoadenine containing DNA; H2O


organism: Homo sapiens


enzyme_display_name: protein-fructosamine 3-kinase

enzyme: protein-fructosamine 3-kinase


enzyme_display_name: UDP-N-acetylglucosamine kinase

organism: Neisseria gonorrhoeae

substrate_name: ATP

assay_conditions_summary: pH 6; 298.15 K; cofactor ATP

enzyme: UDP-N-acetylglucosamine kinase

substrate: ATP


substrate_name: UDP-muramic acid

proof_lines: with UDP-muramic acid, pH 6.0, 25°C, recombinant wild-type enzyme ngzeta_1

proof_lines: with UDP-alpha-D-glucose, pH 6.0, 25°C, recombinant wild-type enzyme ngzeta_1

substrate: UDP-muramic acid


enzyme_display_name: muramoylpentapeptide carboxypeptidase

proof_lines: pH not specified in the publication, temperature not specified in the publication

enzyme: muramoylpentapeptide carboxypeptidase


enzyme_display_name: kallikrein 13

enzyme: kallikrein 13


substrate_name: UDP-N-acetyl-alpha-D-glucosamine

substrate: UDP-N-acetyl-alpha-D-glucosamine


substrate_name: UDP-alpha-D-glucose

substrate: UDP-alpha-D-glucose


substrate_name: H2O; 4-Nitrocatechol sulfate

substrate: H2O; 4-Nitrocatechol sulfate


enzyme_display_name: acyl-homoserine-lactone acylase

organism: Komagataeibacter europaeus

substrate_name: N-3-oxododecanoyl-L-homoserine lactone

enzyme: acyl-homoserine-lactone acylase

substrate: N-3-oxododecanoyl-L-homoserine lactone


enzyme_display_name: trans-L-3-hydroxyproline dehydratase

organism: Colwellia psychrerythraea

enzyme: trans-L-3-hydroxyproline dehydratase


organism: Labrenzia aggregata


organism: Agrobacterium vitis


organism: Azospirillum brasilense


organism: Bacillus thuringiensis


enzyme_display_name: arginine dihydrolase

organism: Anabaena sp.

substrate_name: L-arginine

assay_conditions_summary: pH 8

proof_lines: mutant enzyme R139A, at pH 8.0 and 25Â°C

enzyme: arginine dihydrolase

substrate: L-arginine


proof_lines: wild type full-length enzyme, at pH 8.0 and 25Â°C

proof_lines: wild type N-terminal domain, at pH 8.0 and 25Â°C


organism: Synechocystis sp. PCC 6803


organism: Agrobacterium tumefaciens


organism: Burkholderia ambifaria


organism: Burkholderia thailandensis


organism: Thauera butanivorans

substrate_name: methane

smiles: C

substrate: methane


substrate_name: butane

smiles: CCCC

substrate: butane


substrate_name: ethane

smiles: CC

substrate: ethane


enzyme_display_name: hydroxylamine reductase

organism: Campylobacter curvus

substrate_name: hydroxylamine

proof_lines: oxidation reaction, recombinant MBP-tagged mutant W434Y, pH 7.0, 37°C

proof_lines: reduction reaction, recombinant MBP-tagged mutant W434Y, pH 7.0, 37°C

enzyme: hydroxylamine reductase

substrate: hydroxylamine


organism: Brucella suis biovar 1


sequence_source: UniProtKB:A7GXH2 REST accession lookup on 2026-07-08; sequence not emitted by exact-accession worker

proof_lines: oxidation reaction, recombinant MBP-tagged wild-type enzyme, pH 7.0, 37°C

proof_lines: reduction reaction, recombinant MBP-tagged wild-type enzyme, pH 7.0, 37°C


enzyme_display_name: glycine/sarcosine N-methyltransferase

organism: Aphanothece halophytica

substrate_name: S-adenosyl-L-methionine

sequence_source: UniProtKB:Q83WC4 REST accession lookup on 2026-07-08; sequence not emitted by exact-accession worker

proof_lines: pH 8.8, 37°C, recombinant wild-type enzyme, with sarcosine

proof_lines: pH 8.8, 37°C, recombinant wild-type enzyme, with glycine

enzyme: glycine/sarcosine N-methyltransferase

substrate: S-adenosyl-L-methionine


substrate_name: glycine

sequence_source: UniProtKB:Q83WC4 REST accession lookup on 2026-07-08; canonical identity only, R169K variant sequence not reconstructed

proof_lines: pH 8.8, 37°C, recombinant mutant R169K, with sarcosine

proof_lines: pH 8.8, 37°C, recombinant mutant R169K, with glycine

substrate: glycine


organism: Methanohalophilus portucalensis

sequence_source: UniProtKB:F6KV61 REST accession lookup on 2026-07-08; sequence not emitted by exact-accession worker

proof_lines: pH 7.3, 37°C, recombinant enzyme, with sarcosine

proof_lines: pH 7.3, 37°C, recombinant enzyme, with glycine


enzyme_display_name: glycerone-phosphate O-acyltransferase

organism: Saccharomyces cerevisiae

assay_conditions_summary: cofactor CoA

enzyme: glycerone-phosphate O-acyltransferase


enzyme_display_name: E2 ubiquitin-conjugating enzyme

organism: Homo sapiens

substrate_name: [ubiquitin carrier protein Ubc4]-L-cysteine

enzyme: E2 ubiquitin-conjugating enzyme


proof_lines: about, pH 7.5, temperature not specified in the publication


proof_lines: presence of the U-box domain of E3 enzyme CHIP, 37°C, pH not specified in the publication


enzyme_display_name: IMP-specific 5'-nucleotidase

organism: Plasmodium falciparum

substrate_name: IMP

proof_lines: pH 8, temperature not specified in the publication, wild-type enzyme

proof_lines: pH 5, temperature not specified in the publication, wild-type enzyme

enzyme: IMP-specific 5'-nucleotidase

substrate: IMP


enzyme_display_name: diacylglycerol diphosphate phosphatase

substrate_name: diacylglycerol diphosphate

enzyme: diacylglycerol diphosphate phosphatase

substrate: diacylglycerol diphosphate


organism: Labrenzia aggregata


organism: Micromonospora viridifaciens


substrate_name: Dolichyl phosphate; UDPglucose

substrate: Dolichyl phosphate; UDPglucose


organism: Bellis perennis

substrate_name: UDP-D-glucuronate

assay_conditions_summary: pH 7

proof_lines: pH 7.0, cosubstrate: cyanidin-3-O-(6-O-malonyl-beta-D-glucoside), native enzyme

proof_lines: pH 7.0, cosubstrate: cyanidin-3-O-(6-O-malonyl-beta-D-glucoside), recombinant enzyme

substrate: UDP-D-glucuronate


enzyme_display_name: enterobactin C-glucosyltransferase

organism: Escherichia coli O6:H1

enzyme: enterobactin C-glucosyltransferase


substrate_name: CMP-N-acetylneuraminate

substrate: CMP-N-acetylneuraminate


substrate_name: ganglioside GM3

substrate: ganglioside GM3


proof_lines: 37°C, pH not specified in the publication


substrate_name: [histone H3 peptide 1-15]-L-lysine9

proof_lines: substrate [histone H3 peptide 1-15]-N6-methyl-L-lysine9, SETDB1:ATF7IP complex, pH 7.6, 23°C

substrate: [histone H3 peptide 1-15]-L-lysine9


enzyme_display_name: polysialic-acid O-acetyltransferase

organism: Neisseria meningitidis

substrate_name: acetyl-CoA

enzyme: polysialic-acid O-acetyltransferase

substrate: acetyl-CoA


organism: Rattus norvegicus

substrate_name: Glycerone phosphate; Palmitoyl-CoA

substrate: Glycerone phosphate; Palmitoyl-CoA


organism: Rhizobium meliloti


organism: Shewanella pealeana


enzyme_display_name: selenate reductase (cytochrome c)

organism: Unknown

substrate_name: selenate

enzyme: selenate reductase (cytochrome c)

substrate: selenate


organism: Shewanella woodyi


enzyme_display_name: 4-oxalmesaconate hydratase

organism: Sphingomonas paucimobilis

enzyme: 4-oxalmesaconate hydratase


enzyme_display_name: acetylesterase

organism: Trichoderma reesei

substrate_name: 4-nitrophenyl acetate

proof_lines: purified recombinant enzyme, 30°C, pH 5.8

enzyme: acetylesterase

substrate: 4-nitrophenyl acetate


organism: Drosophila melanogaster

substrate_name: Unknown

substrate: Unknown


organism: Escherichia coli

substrate_name: tributyrin

assay_conditions_summary: pH 7.1; 303.15 K; buffer 20 mM phosphate

proof_lines: mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C

substrate: tributyrin


substrate_name: 4-nitrophenyl butyrate

proof_lines: wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C

substrate: 4-nitrophenyl butyrate


proof_lines: mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C


organism: Acetivibrio thermocellus

assay_conditions_summary: pH 6; 313.15 K; buffer 0.1 M sodium phosphate

proof_lines: isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C


organism: Cellvibrio japonicus

proof_lines: isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C

proof_lines: isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C


organism: Marchantia polymorpha

substrate_name: 2-t-butylcyclohex-1-en-1-yl acetate

proof_lines:
isoenzyme Est I, pH 7.0, 35°C
isoenzyme Est II, pH 7.0, 35°C

substrate: 2-t-butylcyclohex-1-en-1-yl acetate


organism: Helicobacter pylori

substrate_name: H2O; p-Nitrophenyl acetate

substrate: H2O; p-Nitrophenyl acetate


substrate_name: p-Nitrophenyl acetate

substrate: p-Nitrophenyl acetate


organism: Homo sapiens

substrate_name: p-Nitrophenyl acetate; H2O

substrate: p-Nitrophenyl acetate; H2O


organism: Mycobacterium tuberculosis


proof_lines: mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C


proof_lines: mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C


organism: Alicyclobacillus tolerans


enzyme_display_name: lytic cellulose monooxygenase (C4-dehydrogenating)

organism: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

source_db: uniprot

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

source: uniprot


enzyme_display_name: arsenate reductase (donor)

organism: Chrysiogenes arsenatis

substrate_name: arsenate

enzyme: arsenate reductase (donor)

substrate: arsenate


organism: Desulfitobacterium hafniense

substrate_name: tetrachloroethene (with reduced methyl viologen as electron donor)


organism: Desulfitobacterium hafniense (strain Y51)

substrate_name: tetrachloroethene

substrate: tetrachloroethene


organism: Sulfurospirillum multivorans

substrate: tetrachloroethene (with reduced methyl viologen as electron donor)


organism: Escherichia coli (strain K12)

substrate_name: hydroxylamine (at pH 9)

assay_conditions_summary: pH 9

substrate: hydroxylamine (at pH 9)


enzyme_display_name: aliphatic sulfonate oxidoreductase

organism: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

substrate_name: taurine

enzyme: aliphatic sulfonate oxidoreductase

substrate: taurine


organism: Aphanothece halophytica

substrate_name: S-adenosyl-L-homocysteine (at pH 8.8 and at 37 degrees Celsius)

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.


organism: Nicotiana tabacum


organism: Pantoea dispersa


organism: Phocaeicola vulgatus


organism: Mus musculus

substrate_name: histone octamer

substrate: histone octamer


enzyme_display_name: 2-phosphinomethylmalate synthase

organism: Streptomyces hygroscopicus

substrate_name: acetyl-CoA

enzyme: 2-phosphinomethylmalate synthase

substrate: acetyl-CoA


organism: Bellis perennis

substrate_name: cyanidin 3-O-6''-O-malonylglucoside

substrate: cyanidin 3-O-6''-O-malonylglucoside


enzyme_display_name: oleanolate 3-O-glucosyltransferase

organism: Barbarea vulgaris

substrate_name: UDP-glucose

enzyme: oleanolate 3-O-glucosyltransferase

substrate: UDP-glucose


organism: Homo sapiens

substrate_name: CMP-N-acetylneuraminate

substrate: CMP-N-acetylneuraminate


substrate_name: CMP-N-acetyl-beta-neuraminate

substrate: CMP-N-acetyl-beta-neuraminate


substrate_name: N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucose (with a constant CMP-Neu5Ac concentration of 1 mM)


substrate_name: ganglioside GM3

substrate: ganglioside GM3


substrate_name: a neolactoside IV3-alpha-NeuAc-nLc4Cer

substrate: a neolactoside IV3-alpha-NeuAc-nLc4Cer


enzyme_display_name: protein-fructosamine 3-kinase

substrate_name: deoxymorpholinofructose

enzyme: protein-fructosamine 3-kinase

substrate: deoxymorpholinofructose


substrate_name: adenylyl

assay_conditions_summary: cofactor ATP

substrate: adenylyl


substrate_name: p-nitrophenyl acetate

substrate: p-nitrophenyl acetate


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: pNP-acetate

data_origin: experimental

substrate: pNP-acetate


organism: Trichoderma reesei

substrate_name: 4-nitrophenyl acetate

substrate: 4-nitrophenyl acetate


enzyme_display_name: Aspergillus nuclease S1

organism: Aspergillus oryzae (strain ATCC 42149 / RIB 40)

substrate_name: ssDNA

enzyme: Aspergillus nuclease S1

substrate: ssDNA


organism: Arabidopsis thaliana


substrate_name: PtdIns(4,5)P2 (at pH 7.0 and 22 degrees Celsius)


substrate_name: phosphatidylinositol 4,5-bisphosphate

substrate: phosphatidylinositol 4,5-bisphosphate


organism: Rattus norvegicus


substrate_name: 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate (at pH 6.5 and 37 degrees Celsius)


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: Unknown

substrate: Unknown


enzyme_display_name: L-tryptophan decarboxylase

organism: Psilocybe cubensis

substrate_name: L-phenylalanine

enzyme: L-tryptophan decarboxylase

substrate: L-phenylalanine


substrate_name: L-tryptophan

substrate: L-tryptophan


enzyme_display_name: trans-L-3-hydroxyproline dehydratase

enzyme: trans-L-3-hydroxyproline dehydratase


organism: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)


enzyme_display_name: grixazone synthase

organism: Streptomyces griseus subsp. griseus (strain JCM 4626 / CBS 651.72 / NBRC 13350 / KCC S-0626 / ISP 5235)

substrate_name: alpha-aminophenol

proof_lines: kcat is 20 sec(-1) with alpha-aminophenol as substrate. kcat is 14 sec(-1) with 3,4-AHBAL as substrate. kcat is 18 sec(-1) with 2-amino-4-methylphenol as substrate. kcat is 0.8 sec(-1) with 3,4-dihydroxybenzaldehyde as substrate. kcat is 12

enzyme: grixazone synthase

substrate: alpha-aminophenol


enzyme_display_name: 1-hydroxy-2-naphthoate 1,2-dioxygenase

organism: Nocardioides sp. (strain KP7)

proof_lines: kcat is 114 sec(-1) for 1-hydroxy-2-naphthoate.

enzyme: 1-hydroxy-2-naphthoate 1,2-dioxygenase


enzyme_display_name: ammonia monooxygenase

organism: Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)

proof_lines: kcat is 20 sec(-1) for ammonia as substrate.

enzyme: ammonia monooxygenase


organism: Shewanella sp. (strain ANA-3)

substrate_name: arsenate

substrate: arsenate


organism: Methanosarcina thermophila (strain ATCC 43570 / DSM 1825 / OCM 12 / VKM B-1830 / TM-1)

substrate_name: reduced 2-hydroxyphenazine

proof_lines: kcat is 74 sec(-1) at 25 degrees Celsius.

substrate: reduced 2-hydroxyphenazine


organism: Thauera selenatis

substrate_name: selenate (with reduced benzyl viologen as the electron donor)

proof_lines: kcat is 387 sec(-1) (with reduced benzyl viologen as the electron donor).


substrate: selenate (with reduced benzyl viologen as the electron donor)


substrate_name: tRNA(Gly GCC)

proof_lines: kcat is 0.54 min(-1) for tRNA(Gly GCC) and 0.31 min(-1) for tRNA(Val UAC) (m1G9)-methylation, respectively.

substrate: tRNA(Gly GCC)


organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843)

substrate_name: tRNA(Gly)

proof_lines: kcat is 0.16 min(-1) for tRNA(Gly) (m1G9)-methylation.

substrate: tRNA(Gly)


substrate_name: N-palmitoylethanolamine

verification_status: unverified

substrate: N-palmitoylethanolamine

status: unverified


enzyme_display_name: acetylputrescine deacetylase

organism: Danio rerio

substrate_name: acetylcadaverine

enzyme: acetylputrescine deacetylase

substrate: acetylcadaverine


organism: Homo sapiens


organism: Mycoplana ramosa

substrate_name: N-acetylcadaverine

substrate: N-acetylcadaverine


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)


enzyme_display_name: acyl-homoserine-lactone acylase

organism: Komagataeibacter europaeus

substrate_name: 3-oxo-decanoyl homoserine lactone

enzyme: acyl-homoserine-lactone acylase

substrate: 3-oxo-decanoyl homoserine lactone


enzyme_display_name: 6-aminohexanoate-cyclic-dimer hydrolase

organism: Paenarthrobacter ureafaciens

enzyme: 6-aminohexanoate-cyclic-dimer hydrolase


enzyme_display_name: diacylglycerol diphosphate phosphatase

organism: Escherichia coli (strain K12)

substrate_name: undecaprenyl pyrophosphate

enzyme: diacylglycerol diphosphate phosphatase

substrate: undecaprenyl pyrophosphate


enzyme_display_name: galactolipase

organism: Parachlorella kessleri

proof_lines:
pH 6.5, 37°C, recombinant enzyme
pH 6.5, 37°C, native enzyme

enzyme: galactolipase


organism: Arabidopsis thaliana

substrate_name: ssDNA (at pH 7.0 and 37 degrees Celsius)

proof_lines: kcat is 19171 min(-1) with ssDNA as substrate (at pH 7.0 and 37 degrees Celsius).


proof_lines: kcat is 4.8 sec(-1) with 1D-myo-inositol 1,4,5-trisphosphate.


proof_lines: kcat is 0.026 sec(-1) with 1D-myo-inositol 1,4,5-trisphosphate.


proof_lines: kcat is 26 sec(-1) with 1D-myo-inositol 1,4,5-trisphosphate.


enzyme_display_name: IMP-specific 5'-nucleotidase

organism: Plasmodium falciparum (isolate 3D7)

substrate_name: IMP (at pH 5, 25 degrees Celsius and in the absence of ATP)

assay_conditions_summary: pH 5; cofactor ATP

proof_lines: kcat is 10.3 sec(-1) with IMP as substrate (at pH 5, 25 degrees Celsius and in the absence of ATP) (PubMed:32591529). kcat is 13.5 sec(-1) with IMP as substrate (at pH 8, 25 degrees Celsius and in the absence of ATP) (PubMed:32591529). kcat

enzyme: IMP-specific 5'-nucleotidase


enzyme_display_name: (R)-specific secondary-alkylsulfatase (type III)

organism: Pseudomonas sp

substrate_name: (R)-2-octyl sulfate

proof_lines: kcat is 262 min(-1) with (R)-2-octyl sulfate as substrate. kcat is 52 min(-1) with 1-octyl sulfate as substrate.

substrate: (R)-2-octyl sulfate


enzyme_display_name: inosine nucleosidase

substrate_name: uridine

proof_lines: kcat is 2.65 sec(-1) with xanthosine as substrate (PubMed:30787180). kcat is 33.5 sec(-1) with uridine as substrate (PubMed:30787180). kcat is 23.3 sec(-1) with xanthosine as substrate (when in complex with URH2) (PubMed:30787180). kcat is

enzyme: inosine nucleosidase

substrate: uridine


substrate_name: xanthosine (when in complex with URH2)

proof_lines: kcat is 23.3 sec(-1) with xanthosine as substrate (when in complex with URH2) (PubMed:30787180). kcat is 55.3 sec(-1) with uridine as substrate (when in complex with URH2) (PubMed:30787180). kcat is 42.3 sec(-1) with inosine as substrate (w

substrate: xanthosine (when in complex with URH2)


organism: Caenorhabditis elegans

substrate_name: inosine (at pH 7.0 and 37 degrees Celsius)

proof_lines: kcat is 0.79 sec(-1) with inosine as substrate (at pH 7.0 and 37 degrees Celsius) (PubMed:12777783). kcat is 0.203 sec(-1) with inosine as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:28218438). kcat is 0.78 sec(-1) with adenosine a


organism: Crithidia fasciculata

substrate_name: inosine

proof_lines: kcat is 32 sec(-1) with inosine as substrate. kcat is 243 sec(-1) with 5-methyluridine as substrate.

substrate: inosine


organism: Leishmania major

proof_lines: kcat is 119 sec(-1) with inosine as substrate. kcat is 32 sec(-1) with uridine as substrate. kcat is 0.6 sec(-1) with guanosine as substrate. kcat is 0.6 sec(-1) with adenosine as substrate. kcat is 0.4 sec(-1) with cytidine as substrate.


enzyme_display_name: caricain

organism: Carica papaya

proof_lines: kcat is 0.5 sec(-1) with Boc-Ala-Ala-Gly-NHPhNO(2) as substrate (PubMed:2404797). kcat is 0.1 sec(-1) with Boc-Ala-Ala-Gly-NHMec as substrate (PubMed:2404797).

enzyme: caricain


organism: Bos taurus

substrate_name: IMP

substrate: IMP


organism: Gallus gallus

substrate_name: IMP | GMP

substrate: IMP | GMP


substrate_name: AMP | dCMP | IMP

substrate: AMP | dCMP | IMP


substrate_name: AMP | GMP | IMP | dTMP | UMP | CMP | dAMP | NMN


organism: Rattus norvegicus

substrate_name: IMP | dIMP | GMP | dGMP

substrate: IMP | dIMP | GMP | dGMP


organism: Homo sapiens

substrate_name: N-hexadecanoyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine (in the presence of deoxycholic acid) | N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-1,2-diacyl-sn-glycero-3-phosphoethanolamine (in the presence of deoxycholic acid)

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


substrate_name: inosine | adenosine

substrate: inosine | adenosine


enzyme_display_name: cathepsin X

substrate_name: Z-Phe-Arg-NMec

enzyme: cathepsin X

substrate: Z-Phe-Arg-NMec


enzyme_display_name: intramembrane prenyl-peptidase Rce1

substrate_name: farnesyl-Ki-Ras | geranylgeranyl-Ki-Ras

enzyme: intramembrane prenyl-peptidase Rce1

substrate: farnesyl-Ki-Ras | geranylgeranyl-Ki-Ras


substrate_name: N-palmitoylethanolamine

smiles: CCCCCCCCCCCCCCCC(=O)NCCO


enzyme_display_name: photosystem II

organism: Helianthus annuus

substrate_name: plastoquinol

proof_lines: at 21% O2, pH not specified in the publication, temperature not specified in the publication

enzyme: photosystem II

substrate: plastoquinol


organism: Spinacia oleracea

substrate_name: H2O; hn; Plastoquinone

substrate: H2O; hn; Plastoquinone


organism: Pseudaminobacter salicylatoxidans


proof_lines: pH 8.0, temperature not specified in the publication, mutant M46V


proof_lines: pH 8.0, temperature not specified in the publication, mutant W104Y


proof_lines: pH 8.0, temperature not specified in the publication, wild-type enzyme


proof_lines: pH 8.0, temperature not specified in the publication, mutant A85H


proof_lines: pH 8.0, temperature not specified in the publication, mutant L38Q


organism: Pseudomonas sp.

proof_lines:
competitive inhibition
substrate inhibition


enzyme_display_name: linolenate 9R-lipoxygenase

organism: Nostoc sp.

substrate_name: alpha-linolenate

enzyme: linolenate 9R-lipoxygenase

substrate: alpha-linolenate


enzyme_display_name: chymopapain

proof_lines: kcat is 0.5 sec(-1) with Boc-Ala-Ala-Gly-NHPhNO(2) as substrate (PubMed:2404797). kcat is 0.4 sec(-1) with Boc-Ala-Ala-Gly-NHMec as substrate (PubMed:2404797).

enzyme: chymopapain


organism: Methanococcus maripaludis (strain DSM 14266 / JCM 13030 / NBRC 101832 / S2 / LL)

substrate_name: peptide substrate DABCYL-ARSGAKASGC(farnesyl)LVS-EDANS where EDANS is 5-[(2-aminoethyl)amino]naphthalene-1-sulphonic acid fluorophore and DABCYL is 4-{[4-(dimethylamino)phenyl]azo}benzoic acid quencher

proof_lines: kcat is 0.175 sec(-1) for peptide substrate DABCYL-ARSGAKASGC(farnesyl)LVS-EDANS.


enzyme_display_name: N-acetylmuramoyl-L-alanine amidase

organism: Escherichia coli (strain K12)

enzyme: N-acetylmuramoyl-L-alanine amidase


enzyme_display_name: urethanase

organism: Agrobacterium tumefaciens

substrate_name: ethyl carbamate

proof_lines: kcat is 0.81 sec(-1) with ethyl carbamate as substrate.

enzyme: urethanase

substrate: ethyl carbamate


enzyme_display_name: arginine dihydrolase

organism: Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)

substrate_name: arginine

proof_lines: kcat is 271.1 min(-1) with arginine as substrate (for full-length AgrE) (PubMed:32198136). kcat is 292.6 min(-1) with arginine as substrate (for NTD-AgrE) (PubMed:32198136).

enzyme: arginine dihydrolase

substrate: arginine


organism: Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

proof_lines: kcat is 6.4 sec(-1) with arginine as substrate.


enzyme_display_name: L-tryptophan decarboxylase

organism: Clostridium sporogenes (strain ATCC 15579)

substrate_name: tryptophan

proof_lines: kcat is 1200 min(-1) with tryptophan as substrate.

enzyme: L-tryptophan decarboxylase

substrate: tryptophan


organism: Mediterraneibacter gnavus (strain ATCC 29149 / DSM 114966 / JCM 6515 / VPI C7-9)

proof_lines: kcat is 4400 min(-1) with tryptophan as substrate. kcat is 230 min(-1) with phenylalanine as substrate.


organism: Pseudomonas fluorescens

substrate_name: ACMS in presence of Zn(2+)

proof_lines: kcat is 6.65 sec(-1) to 7.3 sec(-1) for ACMS decarboxylation in presence of Co(2+) (PubMed:16131206, PubMed:16716073, PubMed:16939194). kcat is 6.5 sec(-1) to 15.8 sec(-1) for ACMS decarboxylation in presence of Zn(2+) (PubMed:16716073, Pub

substrate: ACMS in presence of Zn(2+)


enzyme_display_name: trans-L-3-hydroxyproline dehydratase

organism: Agrobacterium fabrum (strain C58 / ATCC 33970)

proof_lines: kcat is 27 sec(-1) for t3LHyp dehydration. kcat is 0.40 sec(-1) for t4LHyp epimerization.

enzyme: trans-L-3-hydroxyproline dehydratase


organism: Allorhizobium ampelinum (strain ATCC BAA-846 / DSM 112012 / S4)

proof_lines: kcat is 4.3 sec(-1) for t3LHyp dehydration.


organism: Bacillus cereus (strain ATCC 10987 / NRS 248)

proof_lines: kcat is 1.2 sec(-1) for t4LHyp epimerization. The reaction of t3LHyp dehydration is too slow to measure kinetic parameters.


organism: Bacillus thuringiensis subsp. konkukian (strain 97-27)

proof_lines: kcat is 23 sec(-1) for t3LHyp dehydration. kcat is 0.16 sec(-1) for t4LHyp epimerization. The reaction of t4LHyp epimerization is too slow to measure KM for t4LHyp.


organism: Brucella suis biovar 1 (strain 1330)

proof_lines: kcat is 17 sec(-1) for t3LHyp dehydration.


organism: Burkholderia ambifaria (strain ATCC BAA-244 / DSM 16087 / CCUG 44356 / LMG 19182 / AMMD)

proof_lines: kcat is 43 sec(-1) for t3LHyp dehydration.


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

substrate_name: the dehydration of trans-3-hydroxy-L-proline


organism: Rhizobium meliloti (strain 1021)

proof_lines: kcat is 7.9 sec(-1) for t3LHyp dehydration. kcat is 0.089 sec(-1) for t4LHyp epimerization.


organism: Roseibium aggregatum (strain ATCC 25650 / DSM 13394 / JCM 20685 / NBRC 16684 / NCIMB 2208 / IAM 12614 / B1)

proof_lines: kcat is 15 sec(-1) for t3LHyp dehydration.


organism: Shewanella pealeana (strain ATCC 700345 / ANG-SQ1)

substrate_name: Unknown

proof_lines: kcat is 0.15 sec(-1) for t3LHyp dehydration. The reaction is too slow to measure KM for t3LHyp.

substrate: Unknown


organism: Shewanella woodyi (strain ATCC 51908 / MS32)

proof_lines: kcat is 4.1 sec(-1) for t3LHyp dehydration.


organism: Nostoc sp.

substrate_name: linoleate

substrate: linoleate


substrate_name: alpha-linolenic acid

substrate: alpha-linolenic acid


enzyme_display_name: Oplophorus-luciferin 2-monooxygenase

organism: Oplophorus gracilirostris

substrate_name: coelenterazine

proof_lines:
pH 7.6, native enzyme
pH 7.6, catalytic subunit 19kOLase
pH 7.6, native enzymee

enzyme: Oplophorus-luciferin 2-monooxygenase

substrate: coelenterazine


enzyme_display_name: ammonia monooxygenase

proof_lines: whole cell assay, pH not specified in the publication, temperature not specified in the publication

enzyme: ammonia monooxygenase


organism: Nitrosomonas europaea

substrate_name: ammonia

substrate: ammonia


enzyme_display_name: lytic cellulose monooxygenase (C4-dehydrogenating)

organism: Panus similis

substrate_name: cellotetraose

substrate: cellotetraose


organism: Talaromyces amestolkiae

proof_lines: variant lacking the C-terminal linker-like region, pH 6, 23°C

proof_lines: variant with an additional C-terminal carbohydrate binding domain, pH 6, 23°C

enzyme: lytic cellulose monooxygenase (C4-dehydrogenating)


enzyme_display_name: arsenate reductase (donor)

organism: Chrysiogenes arsenatis

substrate_name: arsenate

enzyme: arsenate reductase (donor)

substrate: arsenate


organism: Chlamydomonas reinhardtii

proof_lines: S0.5 value, pH 7.9, temperature not specified in the publication


substrate_name: 2-mercaptoethanol glutathione disulfide

proof_lines: cosubstrate 2-mercaptoethanol glutathione disulfide, pH not specified in the publication, temperature not specified in the publication

proof_lines: cosubstrate glutathione, S0.5 value, pH not specified in the publication, temperature not specified in the publication

substrate: 2-mercaptoethanol glutathione disulfide


substrate_name: glutathione

proof_lines: pH not specified in the publication, temperature not specified in the publication

proof_lines: pH 7.9, temperature not specified in the publication

substrate: glutathione


organism: Homo sapiens

substrate_name: Reduced glutathione; Glutathionylated RNase A


substrate: Reduced glutathione; Glutathionylated RNase A


substrate_name: Asn-Arg-Cys-Ser-Gln-Gly-Ser-Cys-Trp-Asn

substrate: Asn-Arg-Cys-Ser-Gln-Gly-Ser-Cys-Trp-Asn


enzyme_display_name: flavonol 7-O-beta-glucosyltransferase

organism: Arabidopsis thaliana

substrate_name: anthranilate

enzyme: flavonol 7-O-beta-glucosyltransferase

substrate: anthranilate


substrate_name: curcumin

substrate: curcumin


enzyme_display_name: acetylesterase

organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: palmitoyl-CoA

proof_lines: kcat is 0.00342 sec(-1) with palmitoyl-CoA as substrate.

enzyme: acetylesterase

substrate: palmitoyl-CoA


substrate_name: Insulin

substrate: Insulin


organism: Mus musculus


organism: Populus sp.


proof_lines: mutant C87S, pH not specified in the publication, temperature not specified in the publication


substrate_name: glutathionylated BSA

proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication

substrate: glutathionylated BSA


organism: Rattus norvegicus

substrate_name: GSH

proof_lines:
enzyme from different rat tissues
enzymes from different rat tissues

substrate: GSH


organism: Methanomassiliicoccus luminyensis

substrate_name: coenzyme B-coenzyme M disulfide

substrate: coenzyme B-coenzyme M disulfide


organism: Methanosarcina acetivorans

substrate_name: coenzyme M

proof_lines: subunit HdrB2C2, at 21°C, pH not specified in the publication

proof_lines: subunit HdrB2, at 21°C, pH not specified in the publication

substrate: coenzyme M


organism: Methanosarcina barkeri

proof_lines: pH 7.0, 37°C, reaction with reduced benzyl viologen


organism: Methanosarcina thermophila

substrate_name: dihydromethanophenazine

substrate: dihydromethanophenazine


organism: Methanothermobacter thermautotrophicus

proof_lines: pH 7, 65°C, H2: heterodisulfide oxidoreductase complex


enzyme_display_name: selenate reductase (cytochrome c)

organism: Alcaligenes sp.

substrate_name: selenate

enzyme: selenate reductase (cytochrome c)

substrate: selenate


organism: Desulfitobacterium hafniense

substrate_name: methyl viologen

proof_lines: at a tetrachloroethene concentration of 1 mM

substrate: methyl viologen


organism: Desulfitobacterium sp.

substrate_name: hexachloroethane

substrate: hexachloroethane


organism: Paraclostridium bifermentans

substrate_name: tetrachloroethene

substrate: tetrachloroethene


organism: Sulfurospirillum multivorans

proof_lines: titanium(III)citrate as sole electron donor

proof_lines: methyl viologen as artificial electron donor

proof_lines: reduced 1.6 mM methyl viologen as electron donor


enzyme_display_name: hydroxylamine reductase

organism: Caminibacter mediatlanticus

substrate_name: hydroxylamine

proof_lines: oxidation reaction, recombinant MBP-tagged wild-type enzyme, pH 7.0, 37°C

proof_lines: reduction reaction, recombinant MBP-tagged wild-type enzyme, pH 7.0, 37°C

enzyme: hydroxylamine reductase

substrate: hydroxylamine


substrate_name: nitrite

proof_lines: reduction reaction, recombinant MBP-tagged mutant W434Y, pH 7.0, 37°C

substrate: nitrite


organism: Campylobacter fetus subsp. fetus


organism: Cucurbita pepo

substrate_name: reduced benzyl viologen

substrate: reduced benzyl viologen


organism: Escherichia coli

proof_lines:
at pH 9
at pH 7.5


organism: Pyrococcus furiosus


organism: Vigna radiata var. radiata

substrate_name: dolichyl phosphate

substrate: dolichyl phosphate


organism: Zea mays

substrate_name: UDP-glucose

substrate: UDP-glucose


enzyme_display_name: mannosyl-3-phosphoglycerate synthase

organism: Dehalococcoides mccartyi

enzyme: mannosyl-3-phosphoglycerate synthase


organism: Pyrococcus horikoshii

substrate_name: GDP-mannose

substrate: GDP-mannose


organism: Rhodothermus marinus


organism: Rubrobacter xylanophilus

substrate_name: GDP-glucose

substrate: GDP-glucose


organism: Thermus thermophilus


organism: Beta vulgaris

substrate_name: betanidin

substrate: betanidin


organism: Euonymus alatus

proof_lines:
pH 7.5, 30°C, cosubstrate kaempferol
pH 7.5, 30°C


organism: Glebionis segetum

substrate_name: quercetin

substrate: quercetin


organism: Nicotiana tabacum


organism: Paederia scandens

substrate_name: kaempferol

proof_lines:
pH 7.5
pH 7.5, cosubstrate kaempferol

substrate: kaempferol


organism: Mus musculus

substrate_name: histone K4-acetylK9

substrate: histone K4-acetylK9


organism: Neurospora crassa

substrate_name: biotinyl-MARTKQTARKSTGGKAPRKQ

assay_conditions_summary: pH 9.8; 295.15 K; buffer containing 50 mM glycine (pH 9

proof_lines: in methylation buffer containing 50 mM glycine (pH 9.8), 2 mM dithiothreitol, 0.025 mg/ml bovine serum albumin, 10% (v/v) glycerol, at 22°C

substrate: biotinyl-MARTKQTARKSTGGKAPRKQ


enzyme_display_name: 1-deoxy-D-xylulose-5-phosphate synthase

substrate_name: DXP synthase; D-Glyceraldehyde 3-phosphate

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

proof_lines:
pH 8.0, 37°C
Ki above 0.2 mM, at pH 8.0 and 37°C
Ki above 0.1 mM, at pH 8.0 and 37°C

enzyme: 1-deoxy-D-xylulose-5-phosphate synthase


enzyme_display_name: polysialic-acid O-acetyltransferase

substrate_name: acetyl-CoA

enzyme: polysialic-acid O-acetyltransferase

substrate: acetyl-CoA


proof_lines: mutant NeuO + 24 hepta-nucleotide repeats

proof_lines: mutant NeuO + 12 hepta-nucleotide repeats

proof_lines: mutant NeuO + 36 hepta-nucleotide repeats


enzyme_display_name: meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase I

organism: Mycobacterium tuberculosis

substrate_name: malonyl-[acyl-carrier protein from Escherichia coli]

proof_lines: pH and temperature not specified in the publication


enzyme_display_name: meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase II

organism: Escherichia coli

substrate_name: malonyl-CoA

assay_conditions_summary: pH 8.5; 298.15 K; cofactor CoA

substrate: malonyl-CoA


substrate_name: malonyl-phosphopantetheine 8-mer

enzyme: meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase II

substrate: malonyl-phosphopantetheine 8-mer


substrate_name: C20:0-[acyl-carrier protein from Escherichia coli]


enzyme_display_name: glycerone-phosphate O-acyltransferase

organism: Cavia porcellus

substrate_name: dihydroxyacetone phosphate

assay_conditions_summary: pH 7.4; cofactor CoA

proof_lines:
purified enzyme, pH 7.4
pH 5.5
high concentration of substrate

enzyme: glycerone-phosphate O-acyltransferase

substrate: dihydroxyacetone phosphate


organism: Leishmania major


organism: Oryctolagus cuniculus


organism: Rattus norvegicus

substrate_name: Unknown

substrate: Unknown


organism: Saccharomyces cerevisiae


enzyme_display_name: E2 ubiquitin-conjugating enzyme

organism: Homo sapiens

proof_lines: mutant D576A, pH 7.5, temperature not specified in the publication

enzyme: E2 ubiquitin-conjugating enzyme


proof_lines: mutant D576E, pH 7.5, temperature not specified in the publication


proof_lines: mutant K528A, pH 7.5, temperature not specified in the publication


proof_lines: wild-type, pH 7.5, temperature not specified in the publication


proof_lines: mutant D576N, pH 7.5, temperature not specified in the publication


organism: Acanthamoeba castellanii

substrate_name: dolichyl phosphate


organism: Candida albicans

substrate_name: UDP-glucose


substrate_name: arsenate

proof_lines:
pH 8.5, 30°C, 2 mM glutathione
pH 8.5, 30°C, 5 mM glutathione

substrate: arsenate


organism: Hydrogenophaga sp. NT14

substrate_name: arsenite

proof_lines: pH 5.5, calculated with an Mr of 309000 Da

substrate: arsenite


organism: Hydrogenophaga sp. NT-26


organism: Pyrobaculum aerophilum

proof_lines: isoform Arr-Mo, with methyl viologen as acceptor, at pH 7.5 and 80°C

proof_lines: isoform Arr-W, with methyl viologen as acceptor, at pH 7.5 and 80°C


enzyme_display_name: reticuline oxidase

organism: Arabidopsis thaliana

substrate_name: cellotetraose

enzyme: reticuline oxidase

substrate: cellotetraose


organism: Berberis beaniana

substrate_name: (S)-reticuline

substrate: (S)-reticuline


organism: Eschscholzia californica

sequence_variant_note: Resolved canonical sequence from UniProt P30986 plus row-specific mutation reconstruction and applied mutation signature C166A to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 9; 310.15 K; cofactor FAD


sequence_variant_note: Resolved canonical sequence from UniProt P30986 plus row-specific mutation reconstruction and applied mutation signature Y106F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt P30986 plus row-specific mutation reconstruction and applied mutation signature E417Q to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt P30986 plus row-specific mutation reconstruction and applied mutation signature H459A to reconstruct the assayed variant sequence.


sequence_source: UniProt P30986 PMID-linked direct lookup

proof_lines: pH 9.0, 37°C, recombinant wild-type enzyme


proof_lines: All kinetic experiments are done with 10 mm Tris-HCl, 0.01% Triton X-100, and 10 mm DTT and at pH 8.5.


organism: Escherichia coli O1:K1 / APEC

proof_lines: kcat is 0.51 sec(-1) with acetyl-CoA as substrate. kcat is 1.99 sec(-1) with polymer of sialic acid as substrate.


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: hexadecanoyl-[ACP]

proof_lines: kcat is 5.3 min(-1) with hexadecanoyl-[ACP] as substrate. kcat is 4.5 min(-1) with eicosanoyl-[ACP] as substrate. kcat is 4.8 min(-1) with malonyl-[ACP] as substrate (PubMed:11600501). kcat is 21 min(-1) with malonyl-CoA as substrate. kcat

enzyme: meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase I

substrate: hexadecanoyl-[ACP]


proof_lines: kcat is 1.6 min(-1) with hexadecanoyl-[ACP] as substrate. kcat is 0.6 min(-1) with eicosanoyl-[ACP] as substrate. kcat is 1.4 min(-1) with malonyl-[ACP] as substrate.


substrate_name: beta-catenin-monoubiquitin

proof_lines: Measured for E2-E3 complex composed of neddylated UBE2R1 and the CRL2(FEM1C) complex, using an 'Arg-48' donor ubiquitin.

substrate: beta-catenin-monoubiquitin


substrate_name: acceptor ubiquitin

proof_lines: Measured for E2-E3 complex composed of neddylated UBE2R2 and the CRL2(FEM1C) complex, using an 'Arg-48' donor ubiquitin.

substrate: acceptor ubiquitin


enzyme_display_name: oleanolate 3-O-glucosyltransferase

organism: Barbarea vulgaris

substrate_name: oleanolate

proof_lines: kcat is 0.816 sec(-1) with oleanolate as substrate (PubMed:23027665). kcat is 0.389 sec(-1) with hederagenin as substrate (PubMed:23027665).

enzyme: oleanolate 3-O-glucosyltransferase

substrate: oleanolate


proof_lines: kcat is 0.023 sec(-1) with oleanolate as substrate (PubMed:23027665). kcat is 0.006 sec(-1) with hederagenin as substrate (PubMed:23027665).


enzyme_display_name: enterobactin C-glucosyltransferase

organism: Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

substrate_name: enterobactin

proof_lines: kcat is 11.2 min(-1) with enterobactin as substrate. kcat is 9.7 min(-1) with UDP-Glc as substrate (for the first C-glucosylation).

enzyme: enterobactin C-glucosyltransferase

substrate: enterobactin


enzyme_display_name: corrinoid adenosyltransferase EutT

organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

substrate_name: ATP

enzyme: corrinoid adenosyltransferase EutT

substrate: ATP


enzyme_display_name: 5-aminovalerate transaminase

organism: Escherichia coli (strain K12)

substrate_name: 4-aminobutanoate (measured for a coupled GabT/GabD reaction)

proof_lines: kcat is 47.4 sec(-1) with 4-aminobutanoate as substrate (at 25 degrees Celsius and pH 7.8).

enzyme: 5-aminovalerate transaminase


enzyme_display_name: gentamicin 2''-nucleotidyltransferase

organism: Klebsiella pneumoniae

substrate_name: kanamycin B

proof_lines: kcat is 15.3 sec(-1) for ATP and 5.2 sec(-1) for kanamycin B.

enzyme: gentamicin 2''-nucleotidyltransferase

substrate: kanamycin B


organism: Alcaligenes sp. CKCr-6A

substrate_name: selenate

substrate: selenate


organism: Enterobacter cloacae

proof_lines:
using electron donormethyl viologen
20°C
with reduced benzyl viologen, 20°C


organism: Sulfurospirillum barnesii


organism: Thauera selenatis

assay_conditions_summary: pH 6

proof_lines: tungsten-SER, pH and temperature not specified in the publication, Vmax: 0.01 micromol/min/mg

proof_lines: molybdenum-SER, pH and temperature not specified in the publication, Vmax: 0.2 micromol/min/mg


enzyme_display_name: glycine/sarcosine N-methyltransferase

organism: Aphanothece halophytica

substrate_name: N-methylglycine

sequence_source: UniProtKB:Q83WC4 (PMID 12466265)

proof_lines: with N-methylglycine as methyl group acceptor, pH 8.8, 37°C

proof_lines: with glycine as methyl group acceptor, pH 8.8, 37°C

enzyme: glycine/sarcosine N-methyltransferase

substrate: N-methylglycine


organism: Halorhodospira halochloris

substrate_name: S-adenosyl-L-methionine

sequence_source: UniProtKB:Q9KJ22 (PMID 11319079)

proof_lines: recombinant enzyme, pH 7.4, 37°C, with sarcosine

proof_lines: recombinant enzyme, pH 7.4, 37°C, with S-adenosyl-L-methionine

proof_lines: recombinant enzyme, pH 7.4, 37°C, with glycine

substrate: S-adenosyl-L-methionine


organism: Galdieria sulphuraria

substrate_name: N,N-dimethylglycine

sequence_source: RCSB PDB:2O57 entity 1

proof_lines:
cosubstrate: N,N-dimethylglycine
cosubstrate: sarcosine

substrate: N,N-dimethylglycine


sequence_source: UniProtKB:Q9KJ21 (PMID 11319079)


organism: Methanohalophilus portucalensis

substrate_name: sarcosine

proof_lines: cosubstrate N,N-dimethylglycine, pH 7.2, 37°C

substrate: sarcosine


enzyme_display_name: flavonoid 4'-O-methyltransferase

organism: Camptotheca acuminata

enzyme: flavonoid 4'-O-methyltransferase


organism: Dianthus caryophyllus

substrate_name: apigenin

substrate: apigenin


organism: Mus musculus

substrate_name: histone H3 (T11phos)

substrate: histone H3 (T11phos)


organism: Bellis perennis

substrate_name: cyanidin 3-O-beta-glucoside

substrate: cyanidin 3-O-beta-glucoside


substrate_name: UDP-beta-D-glucuronate

substrate: UDP-beta-D-glucuronate


organism: Entada phaseoloides

substrate_name: luteolin

substrate: luteolin


organism: Panax ginseng

substrate_name: chikusetsusaponin IVa

substrate: chikusetsusaponin IVa


organism: Salmonella enterica

substrate_name: MECAM

proof_lines:
pH 9.0, 5mM MgCl2, 22°C
pH9.0, 5mM MgCl2, 22°C
pH 9.0, 5mM MgCl2, 22 °C

substrate: MECAM


organism: Bos taurus

substrate_name: CMP-N-glycolylneuraminate

proof_lines: ovine, CMP-N-acetylneuraminate as donor, 37°C

substrate: CMP-N-glycolylneuraminate


enzyme_display_name: phosphonoacetaldehyde hydrolase

organism: Achromobacter aegrifaciens

substrate_name: phosphonoacetaldehyde

enzyme: phosphonoacetaldehyde hydrolase

substrate: phosphonoacetaldehyde


organism: Achromobacter insolitus

proof_lines:
at pH 7.5 and 25°C
isoform PhnX19-II, at pH 7.5 and 25°C
isoform PhnX19-I, at pH 7.5 and 25°C


organism: Bacillus cereus


enzyme_display_name: galactolipase

organism: Oryza sativa

substrate_name: monogalactosyldiacylglycerol

enzyme: galactolipase

substrate: monogalactosyldiacylglycerol


organism: Phaseolus coccineus

substrate_name: digalactosyldiacylglycerol

substrate: digalactosyldiacylglycerol


organism: Phaseolus vulgaris

substrate_name: phosphatidylcholine

substrate: phosphatidylcholine


organism: Solanum tuberosum


organism: Vigna unguiculata


organism: Homo sapiens

proof_lines: pH and temperature not specified in the publication


organism: Ovis aries

substrate_name: submaxillary asialo-mucin

proof_lines: ovine, calculated in terms of N-acetylgalactosamine acceptor sites, 37°C

substrate: submaxillary asialo-mucin


organism: Rattus norvegicus

substrate_name: CMP-N-acetylneuraminate

substrate: CMP-N-acetylneuraminate


organism: Sus scrofa

proof_lines: pH 6.5, 37°C, ovine submaxillary asialo-mucin as acceptor

proof_lines: pH 6.5, 37°C, antifreeze glycoprotein 8 as acceptor


organism: Escherichia coli


organism: Gallus gallus

substrate_name: Ganglioside LM1

substrate: Ganglioside LM1


organism: Neisseria meningitidis

proof_lines: calculated and determined at constant donor concentration of 1 mM CMPNeu5Ac


substrate_name: ganglioside GM3

substrate: ganglioside GM3


enzyme_display_name: N-acylneuraminate-9-phosphate synthase

substrate_name: N-acetyl-D-mannosamine 6-phosphate

enzyme: N-acylneuraminate-9-phosphate synthase

substrate: N-acetyl-D-mannosamine 6-phosphate


enzyme_display_name: acetylesterase

organism: Orpinomyces sp.

substrate_name: p-nitrophenyl acetate

enzyme: acetylesterase

substrate: p-nitrophenyl acetate


organism: Pseudomonas putida

substrate_name: 4-nitrophenyl acetate

substrate: 4-nitrophenyl acetate


organism: Rhodococcus sp. (in: high G+C Gram-positive bacteria)

substrate_name: phenyl acetate

proof_lines:
recombinant enzyme, pH 6.4
recombinant enzyme, pH 8.0

substrate: phenyl acetate


organism: Rhodotorula mucilaginosa

substrate_name: acetylxylan

substrate: acetylxylan


organism: Streptomyces sp.


organism: Talaromyces pinophilus


substrate_name: 4-nitrophenyl butyrate

substrate: 4-nitrophenyl butyrate


organism: Thermotoga maritima

proof_lines: in 50 mM citrate-phosphate (pH 6) at 70°C


organism: Trypanosoma brucei

substrate_name: methylumbelliferone acetate

substrate: methylumbelliferone acetate


assay_conditions_summary: pH 7.1; 298.15 K; buffer 20 mM phosphate

proof_lines: pH 7.1, 25°C, recombinant wild-type enzyme

proof_lines: pH 7.1, 65°C, recombinant wild-type enzyme

proof_lines: wild type enzyme, in 20 mM phosphate buffer, at pH 7.1 and 30°C


assay_conditions_summary: pH 7.1; 303.15 K; buffer phosphate

proof_lines: mutant enzyme L97F, in 20 mM phosphate buffer, at pH 7.1 and 30°C


proof_lines: mutant enzyme L97F/L209F, in 20 mM phosphate buffer, at pH 7.1 and 30°C


proof_lines: mutant enzyme L209F, in 20 mM phosphate buffer, at pH 7.1 and 30°C


organism: Exiguobacterium antarcticum

substrate_name: 4-nitrophenyl hexanoate

substrate: 4-nitrophenyl hexanoate


organism: Fibrobacter succinogenes

substrate_name: Alpha-naphthyl acetate

substrate: Alpha-naphthyl acetate


organism: Kluyveromyces marxianus

substrate_name: (R)-1,2-O-isopropylidene glycol acetate

substrate: (R)-1,2-O-isopropylidene glycol acetate


organism: Lactiplantibacillus plantarum

substrate_name: Triacetin

substrate: Triacetin


organism: Lichtheimia corymbifera


organism: Meyerozyma guilliermondii


organism: Nocardia sp.

substrate_name: ethyl acetate

substrate: ethyl acetate


enzyme_display_name: acylcarnitine hydrolase

organism: Mus musculus

enzyme: acylcarnitine hydrolase


organism: Rattus norvegicus

substrate_name: H2O; L-Octanoylcarnitine

proof_lines:
low substrate concentration
high substrate concentration

substrate: H2O; L-Octanoylcarnitine


enzyme_display_name: sialate O-acetylesterase

organism: Bos taurus

substrate_name: 4-yethylumbelliferyl acetate

enzyme: sialate O-acetylesterase

substrate: 4-yethylumbelliferyl acetate


organism: Equus caballus

substrate_name: N-acetyl-9-O-acetylneuraminic acid

substrate: N-acetyl-9-O-acetylneuraminic acid


organism: Equus sp.

substrate_name: 4-O-acetyl-sialic acid

proof_lines: low pI esterase form, obtained with free sialic acid

proof_lines: high pI esterase form, obtained with free sialic acid

substrate: 4-O-acetyl-sialic acid


organism: influenza C virus

substrate_name: N-acetyl-9-O-lactoylneuraminic acid

substrate: N-acetyl-9-O-lactoylneuraminic acid


assay_conditions_summary: pH 7.5; 310.15 K; cofactor CoA


organism: Acinetobacter sp.

proof_lines: pH 7.0, 25°C, presence of benzethonium chloride

proof_lines: pH 7.0, 25°C, absence of benzethonium chloride


organism: Aspergillus niger


organism: Aspergillus oryzae


organism: Bacillus pumilus

substrate_name: 1-naphthol acetate

substrate: 1-naphthol acetate


substrate_name: H2O; p-Nitrophenyl acetate

substrate: H2O; p-Nitrophenyl acetate


enzyme_display_name: phosphomevalonate kinase

substrate_name: Unknown

enzyme: phosphomevalonate kinase

substrate: Unknown


organism: Streptococcus pneumoniae

substrate_name: ATP

assay_conditions_summary: pH 7; 298.15 K; cofactor ATP

substrate: ATP


organism: Sus scrofa

substrate_name: phosphomevalonate

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

proof_lines:
pH 7.5, 30°C
ATP in form of MgATP2-

substrate: phosphomevalonate


organism: Escherichia coli

substrate_name: adenylyl-[glutamine synthase]

assay_conditions_summary: pH 7.6; 303.15 K; cofactor ATP

proof_lines: adenylyl-removing activity, 0.00002 mM ATase, 1 mM alpha-ketoglutarate, 0.0005 mM signal transduction protein PII-UMP, 1 mM ATP, 5 mM potassium phosphate

proof_lines: adenylation of Mycobacterium tuberculosis [L-glutamate:ammonia ligase (ADP-forming)] expressed in Escherichia coli

substrate: adenylyl-[glutamine synthase]


substrate_name: dATP

assay_conditions_summary: pH 9; 298.15 K; cofactor ATP

proof_lines:
pH 9.0, 25°C
dATP in form of MgdATP2-
dGTP in form of MgdGTP2-

substrate: dATP


enzyme_display_name: streptomycin 3''-adenylyltransferase

organism: Bacillus subtilis

substrate_name: streptomycin

enzyme: streptomycin 3''-adenylyltransferase

substrate: streptomycin


organism: Serratia marcescens

proof_lines: kinetic parameter of the ANT(3)-Ii domain of the enzyme

proof_lines: Kii = 0.01 mM, dead-end inhibition of ANT(3)-Ii domain, substrate 0.1 mM ATP, uncompetitive inhibition


enzyme_display_name: amine sulfotransferase

organism: Cavia porcellus

proof_lines: with 2-naphthylamine as second substrate, pH 8, 37°C

enzyme: amine sulfotransferase


organism: Homo sapiens

proof_lines: biphasic kinetics, Km: 0.00013 mM and 0.0022 mM


organism: Mus musculus


organism: Rattus norvegicus


substrate_name: Phosphoenolpyruvate; H2O; N-Acetyl-D-mannosamine 6-phosphate


substrate_name: N-acetyl-D-mannosamine 6-phosphate

substrate: N-acetyl-D-mannosamine 6-phosphate


organism: Anaerocolumna aminovalerica

substrate_name: delta-aminovaleric acid

substrate: delta-aminovaleric acid


organism: Meyerozyma guilliermondii

proof_lines: pH 7.8, 35°C, noncompetitive inhibition with respect to delta-aminovalerate

proof_lines: pH 7.8, 35°C, competitive inhibition with respect to 2-oxoglutarate


organism: Pseudomonas sp.


enzyme_display_name: protein-fructosamine 3-kinase

enzyme: protein-fructosamine 3-kinase


enzyme_display_name: fatty acid kinase

organism: Spirochaetales

substrate_name: valerate

proof_lines: with isobutyrate as cosubstrate, at pH 7.5 and 30°C

enzyme: fatty acid kinase

substrate: valerate


assay_conditions_summary: pH 7; 303.15 K; cofactor ATP


proof_lines:
mutant R84M, 30°C, pH 7.0
mutant R84M, 30°C, pH 8.0


proof_lines:
mutant R111M, 30°C, pH 7.0
mutant R111M, 30°C, pH 8.0


enzyme_display_name: inosine nucleosidase

organism: Azotobacter vinelandii

substrate_name: Xanthosine

enzyme: inosine nucleosidase

substrate: Xanthosine


organism: Helianthus tuberosus

substrate_name: Inosine

substrate: Inosine


organism: Lupinus luteus


organism: Saccharolobus solfataricus

substrate_name: adenosine

substrate: adenosine


enzyme_display_name: DNA-3-methyladenine glycosylase II

substrate_name: duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine

assay_conditions_summary: pH 6.1; 310.15 K; buffer 50 mM Na-MES

proof_lines: at 37°C, in 50 mM Na-MES buffer (pH 6.1)

proof_lines: pH 8.0, 37°C, excision of 5-formyluracil

proof_lines: pH 8.0, 37°C, excision of 7-methylguanine

enzyme: DNA-3-methyladenine glycosylase II


substrate_name: 1,N6-ethenoadenine residues in alkylated DNA

proof_lines: pH 7.5, 37°C, excision of1, N6-ethenoadenine

proof_lines: pH 7.5, 37°C, excision of 1, N6-ethenoadenine


proof_lines:
37°C, pH 7.9
37°C, pH 7.9, presence of 250 mM Mg2+
37°C, pH 7.9, presence of 500 mM Mg2+

substrate: duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine


mutation_type: deletion

proof_lines: pH 8.3, 37°C, release of 1,N6-ethenoadenine, truncated mutant N-DELTA100C-DELTA18

substrate: 1,N6-ethenoadenine residues in alkylated DNA


proof_lines: pH 8.3, 37°C, release of 1,N6-ethenoadenine, wild-type

proof_lines: pH 8.3, 37°C, release of 1,N6-ethenoadenine, wild type enzyme


enzyme_display_name: peptidyl-dipeptidase Dcp

enzyme: peptidyl-dipeptidase Dcp


enzyme_display_name: muramoylpentapeptide carboxypeptidase

enzyme: muramoylpentapeptide carboxypeptidase


organism: Enterococcus faecalis


organism: Bifidobacterium bifidum


organism: Clostridium perfringens


organism: Prunus dulcis


organism: Tannerella forsythia

substrate_name: 4-nitrophenyl alpha-L-fucopyranoside

substrate: 4-nitrophenyl alpha-L-fucopyranoside


enzyme_display_name: isoamylase

organism: Lederbergia lenta

substrate_name: glycogen

enzyme: isoamylase

substrate: glycogen


organism: Bos taurus

substrate_name: nitrocatechol sulfate

substrate: nitrocatechol sulfate


organism: Gallus gallus

substrate_name: UDP-N-acetylgalactosamine 4-sulfate

substrate: UDP-N-acetylgalactosamine 4-sulfate


organism: Homo sapiens

substrate_name: 4-Methylumbelliferyl sulfate

substrate: 4-Methylumbelliferyl sulfate


organism: Mus musculus

substrate_name: p-nitrocatechol sulfate

proof_lines: pH 6.0, 37°C, Schistosoma-infected animals

substrate: p-nitrocatechol sulfate


organism: Rattus norvegicus


enzyme_display_name: (R)-specific secondary-alkylsulfatase (type III)

organism: Pseudomonas sp.

substrate_name: (5R)-nonan-5-yl sulfate

substrate: (5R)-nonan-5-yl sulfate


enzyme_display_name: beta-primeverosidase

organism: Aspergillus fumigatus

enzyme: beta-primeverosidase


organism: Camellia sinensis

substrate_name: 2-phenylethyl beta-vicianoside

substrate: 2-phenylethyl beta-vicianoside


organism: Penicillium multicolor

substrate_name: p-nitrophenyl beta-primeveroside

substrate: p-nitrophenyl beta-primeveroside


organism: Aspergillus niger


organism: Bacillus fulminans


enzyme_display_name: deoxyribonuclease I

organism: Basidiobolus haptosporus

substrate_name: Unknown

enzyme: deoxyribonuclease I

substrate: Unknown


organism: Cyprinus carpio


substrate_name: d(pGpGpCpApCpTpTpApC)

proof_lines: 20 mM Tris-HCl pH 7.5, 5 mM MgCl2, 1 mM EDTA, 37°C

substrate: d(pGpGpCpApCpTpTpApC)


organism: Potyvirus sp.

substrate_name: lambda phage DNA

substrate: lambda phage DNA


enzyme_display_name: Aspergillus nuclease S1

organism: Aspergillus oryzae

enzyme: Aspergillus nuclease S1


substrate_name: denaturated DNA

substrate: denaturated DNA


organism: Neurospora crassa


organism: Aquarana catesbeiana

substrate_name: 4-nitrophenyl phosphate

substrate: 4-nitrophenyl phosphate


organism: Echinococcus granulosus

proof_lines: pH and temperature not specified in the publication


organism: Enterobacter asburiae

assay_conditions_summary: pH 3.5; 310.15 K; cofactor ATP

proof_lines: K0.5 value, Hill coefficient 1.2, pH 3.5, 37°C

proof_lines: K0.5 value, Hill coefficient 1.6, pH 3.5, 37°C

proof_lines: K0.5 value, Hill coefficient 2.3, pH 3.5, 37°C


organism: Escherichia coli

proof_lines: about, pH 7.8, 37°C, inhibition of 4-nitrophenyl phosphatase activity


organism: Fasciola gigantica


organism: Joyeuxiella pasqualei


organism: Leishmania donovani


organism: Leishmania tropica


organism: Macrobrachium olfersii

substrate_name: K+

proof_lines:
pH 7.5, 25°C, 21% salinity
pH 7.5, 25°C, fresh water
pH 7.5, 25°C

substrate: K+


organism: Moniezia expansa


organism: Rattus norvegicus

substrate_name: H2O; 4-Nitrophenyl phosphate

substrate: H2O; 4-Nitrophenyl phosphate


organism: Rodentolepis nana


organism: Toxocara cati


proof_lines: in the presence of 5 mM MgCl2, 0.5 mM EGTA, 50 mM Tris, pH 7.4, temperature not specified in the publication


organism: Trypanosoma brucei

proof_lines: mutant enzyme D360A/N362A, at pH 7.5 and 25°C


organism: Enterococcus gallinarum


organism: Priestia megaterium


organism: Streptomyces albus

substrate_name: diacetyl-L-diaminobutyryl-D-Ala-D-Ala

substrate: diacetyl-L-diaminobutyryl-D-Ala-D-Ala


organism: Streptomyces sp.


enzyme_display_name: cathepsin X

organism: Bos taurus

substrate_name: glucagon

enzyme: cathepsin X

substrate: glucagon


organism: Homo sapiens


substrate_name: hippuryl-L-Arg

substrate: hippuryl-L-Arg


enzyme_display_name: kallikrein 13

substrate_name: Abz-KLKSSKQ-EDDnp

proof_lines: in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C

proof_lines: in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C

enzyme: kallikrein 13

substrate: Abz-KLKSSKQ-EDDnp


enzyme_display_name: caricain

organism: Carica papaya

substrate_name: Succinyl-albumin

enzyme: caricain

substrate: Succinyl-albumin


enzyme_display_name: gingipain R

organism: Porphyromonas gingivalis

substrate_name: prothrombin

proof_lines: HRgpA, pH 7.6, 37°C, in presence of Ca2+ and phospholipids

proof_lines: RgpB, pH 7.6, 37°C, in presence of Ca2+ and phospholipids

enzyme: gingipain R

substrate: prothrombin


enzyme_display_name: staphopain

organism: Staphylococcus aureus

substrate_name: N-Suc-Gly-Phe-Gly-p-nitroanilide

proof_lines: used for inhibition studies of staphopain identified from a novel staphylococcal thiol protease operon stpAB2CH-91

enzyme: staphopain

substrate: N-Suc-Gly-Phe-Gly-p-nitroanilide


organism: Lederbergia lenta

substrate_name: glycogen

substrate: glycogen


organism: Oryza sativa

substrate_name: amylopectin

substrate: amylopectin


organism: Rhizopus arrhizus


organism: Arabidopsis thaliana

substrate_name: uridine

proof_lines: recombinant enzyme expressed in Escherichia coli

substrate: uridine


enzyme_display_name: chymopapain

proof_lines: at pH 7.4, in 100 mM phosphate-buffered saline

enzyme: chymopapain


enzyme_display_name: asclepain

organism: Asclepias curassavica

assay_conditions_summary: pH 8; 310.15 K; buffer 0.1 M Tris-HCl

proof_lines: at 37°C, in 0.1 M Tris-HCl buffer (pH 8.0) containing 2 mM EDTA and 25 mM cysteine

enzyme: asclepain


organism: Asclepias syriaca

substrate_name: N-carbobenzoxy-Lys p-nitrophenyl ester

proof_lines:
asclepain A5
asclepain A2
asclepain B5

substrate: N-carbobenzoxy-Lys p-nitrophenyl ester


enzyme_display_name: gastricsin

enzyme: gastricsin


organism: Sus scrofa

proof_lines:
Km-value for the range 0.025-0.15 mM
Km-value for the range 0.1-0.5 mM
Km-value for the range 0.1-2.0 mM


enzyme_display_name: phytepsin

organism: Cynara cardunculus

enzyme: phytepsin


organism: Cynara cardunculus var. scolymus

assay_conditions_summary: pH 5


organism: Triticum aestivum


enzyme_display_name: leishmanolysin

organism: Leishmania sp.

substrate_name: Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr

enzyme: leishmanolysin

substrate: Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr


enzyme_display_name: intramembrane prenyl-peptidase Rce1

organism: Saccharomyces cerevisiae

substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication

enzyme: intramembrane prenyl-peptidase Rce1

substrate: Unknown


enzyme_display_name: N-acetylmuramoyl-L-alanine amidase

organism: Citrobacter freundii

assay_conditions_summary: pH 7

proof_lines: at pH 7.0, temperature not specified in the publication

enzyme: N-acetylmuramoyl-L-alanine amidase


organism: Escherichia coli


assay_conditions_summary: pH 7; 310.15 K; buffer 50 mM sodium phosphate

proof_lines: in 50 mM sodium phosphate buffer (pH7.0) at 37°C


organism: Saccharolobus solfataricus

substrate_name: maize amylopectin

substrate: maize amylopectin


organism: Homo sapiens


organism: Mus musculus


organism: Staphylococcus epidermidis


organism: Streptomyces globisporus


enzyme_display_name: glutamin-(asparagin-)ase

organism: Achromobacter sp.

substrate_name: L-glutamine

enzyme: glutamin-(asparagin-)ase

substrate: L-glutamine


organism: Acinetobacter glutaminasificans

proof_lines: competitive inhibition of glutaminase activity


organism: Aspergillus niger

substrate_name: L-asparagine

substrate: L-asparagine


proof_lines:
asparaginase B
asparaginase A


organism: Delftia acidovorans


organism: Pseudomonas sp.


organism: Rhodotorula toruloides

substrate_name: N-carbamoyl-L-asparagine

substrate: N-carbamoyl-L-asparagine


organism: Streptomyces canarius

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Tilachlidium humicola

proof_lines: mixed type of competitive and non-competitive inhibition


organism: Mus sp.

substrate_name: oleamide

substrate: oleamide


organism: Rattus norvegicus

substrate_name: N-oleoylethanolamine

proof_lines:
mitochondrial enzyme
mitochondrial

substrate: N-oleoylethanolamine


enzyme_display_name: acetylputrescine deacetylase

organism: Micrococcus luteus

substrate_name: N-acetylputrescine

enzyme: acetylputrescine deacetylase

substrate: N-acetylputrescine


enzyme_display_name: urethanase

organism: Bacillus licheniformis

substrate_name: urethane

proof_lines:
strain IFO 12107
strain 1013, at pH 7.5
strain 1013, at pH 4.5

enzyme: urethanase

substrate: urethane


organism: Citrobacter sp.


organism: Micrococcus sp.


organism: Paenibacillus thiaminolyticus


organism: Prescottella equi


organism: Purpureocillium lilacinum


organism: Talaromyces variabilis


enzyme_display_name: acyl-homoserine-lactone acylase

organism: Delftia sp. VM4

substrate_name: N-3-oxo-hexanoyl-L-homoserine lactone

enzyme: acyl-homoserine-lactone acylase

substrate: N-3-oxo-hexanoyl-L-homoserine lactone


enzyme_display_name: L-tryptophan decarboxylase

organism: Bacillus sp. (in: firmicutes)

substrate_name: L-tryptophan

enzyme: L-tryptophan decarboxylase

substrate: L-tryptophan


organism: Camptotheca acuminata


organism: Catharanthus roseus

proof_lines: at pH 7.5, temperature not specified in the publication


organism: Gelatoporia subvermispora


organism: Vinca minor


organism: Withania coagulans


organism: Felis catus


organism: Homo sapiens

proof_lines: in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C


organism: Pseudomonas fluorescens

assay_conditions_summary: pH 7; buffer pH 7

proof_lines: alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase loaded with 1 molar equivalent Mn2+

proof_lines: alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase loaded with 1 molar equivalent Cd2+

proof_lines: in 25 mM HEPES buffer pH 7.0, the enzyme contains Co2+


assay_conditions_summary: pH 7; 295.15 K; buffer containing 5% glycerol, pH 7

proof_lines: mutant enzyme H228A, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C


sequence_variant_note: Resolved canonical sequence from UniProt Q83V25 ACMSD_PSEFL, supported by RCSB 4IFK/4IFO/4IFR/4IG2 and PMID 24019523 family context and applied mutation signature H177A to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme H177A, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C


sequence_variant_note: Resolved canonical sequence from UniProt Q83V25 ACMSD_PSEFL, supported by RCSB 4IFK/4IFO/4IFR/4IG2 and PMID 24019523 family context and applied mutation signature H9E to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme H9E, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C


sequence_variant_note: Resolved canonical sequence from UniProt Q83V25 ACMSD_PSEFL, supported by RCSB 4IFK/4IFO/4IFR/4IG2 and PMID 24019523 family context and applied mutation signature D294E to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme D294E, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C


sequence_variant_note: Resolved canonical sequence from UniProt Q83V25 ACMSD_PSEFL, supported by RCSB 4IFK/4IFO/4IFR/4IG2 and PMID 24019523 family context and applied mutation signature H228E to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme H228E, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C


sequence_source: UniProt Q83V25 ACMSD_PSEFL, supported by RCSB 4IFK/4IFO/4IFR/4IG2 and PMID 24019523

proof_lines: wild type enzyme, in 25 mM HEPES buffer containing 5% glycerol, pH 7.0, at 22°C


organism: Sus scrofa


enzyme_display_name: phosphonopyruvate decarboxylase

organism: Bacteroides fragilis

enzyme: phosphonopyruvate decarboxylase


enzyme_display_name: 2-hydroxyacyl-CoA lyase

organism: Listeria monocytogenes

substrate_name: formaldehyde

enzyme: 2-hydroxyacyl-CoA lyase

substrate: formaldehyde


enzyme_display_name: trans-L-3-hydroxyproline dehydratase

organism: Azospirillum brasilense

enzyme: trans-L-3-hydroxyproline dehydratase


organism: Rattus norvegicus


enzyme_display_name: 4-oxalmesaconate hydratase

organism: Phytopseudomonas straminea

enzyme: 4-oxalmesaconate hydratase


organism: Pseudomonas aeruginosa

substrate_name: N-octanoyl-L-homoserine lactone

proof_lines: in 0.1 M phosphate buffer pH 7.4, at 30°C

substrate: N-octanoyl-L-homoserine lactone


enzyme_display_name: 6-aminohexanoate-cyclic-dimer hydrolase

organism: Flavobacterium sp.

enzyme: 6-aminohexanoate-cyclic-dimer hydrolase


organism: Paenarthrobacter ureafaciens

substrate_name: 6-amino-hexanoate cyclic dimer

verification_status: corrected

sequence_source: UniProtKB:P13398; RCSB 3A2P/3A2Q SIFTS mapping to P13398; PMID 19889645 Table 3

substrate: 6-amino-hexanoate cyclic dimer

status: corrected


sequence_variant_note: Resolved canonical sequence from UniProtKB:P13398; RCSB 3A2P/3A2Q SIFTS mapping to P13398; PMID 19889645 Table 3 and applied mutation signature N125A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P13398; RCSB 3A2P/3A2Q SIFTS mapping to P13398; PMID 19889645 Table 3 and applied mutation signature C316G to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P13398; RCSB 3A2P/3A2Q SIFTS mapping to P13398; PMID 19889645 Table 3 and applied mutation signature C316S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P13398; RCSB 3A2P/3A2Q SIFTS mapping to P13398; PMID 19889645 Table 3 and applied mutation signature C316D to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P13398; RCSB 3A2P/3A2Q SIFTS mapping to P13398; PMID 19889645 Table 3 and applied mutation signature C316A to reconstruct the assayed variant sequence.


organism: Pseudomonas sp.

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


enzyme_display_name: arginine dihydrolase

organism: Streptomyces sp.

substrate_name: L-arginine

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

enzyme: arginine dihydrolase

substrate: L-arginine


substrate_name: L-citrulline

substrate: L-citrulline


enzyme_display_name: diacylglycerol diphosphate phosphatase

organism: Saccharomyces cerevisiae

substrate_name: diacylglycerol diphosphate

enzyme: diacylglycerol diphosphate phosphatase

substrate: diacylglycerol diphosphate


enzyme_display_name: phenylalanine 3-monooxygenase

organism: Streptomyces coeruleorubidus

substrate_name: L-phenylalanine

proof_lines: pH 8.0, 28°C, cofactor: 6-methyl-5,6,7,8-tetrahydropterin

proof_lines: pH 8.0, 28°C, cofactor: 6,7-dimethyl-5,6,7,8-tetrahydropterin

enzyme: phenylalanine 3-monooxygenase

substrate: L-phenylalanine


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

assay_conditions_summary: pH 3

proof_lines: kcat is 1.3 min(-1) for L-phenylalanine as substrate. kcat is 1.2 min(-1) for 6-methyltetrahydropterin as substrate.


enzyme_display_name: HslU--HslV peptidase

organism: Escherichia coli (strain K12)

substrate_name: Arc-MYL-st11 (at 37 degrees Celsius)

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

assay_conditions_summary: cofactor ATP

proof_lines: Arc is a repressor protein, Arc-MYL-st11 is a hyperstable variant of Arc.

enzyme: HslU--HslV peptidase

substrate: Arc-MYL-st11 (at 37 degrees Celsius)


organism: Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

proof_lines: Arc-H(6)-SulA(CT) is a construct consisting of the Arc repressor protein fused to 6 His residues and the 11 carboxy terminal residues of SulA.


protein_accession_database: NCBI Protein

sequence_variant_note: Resolved canonical sequence from genbank_protein_reconstructed_variant:BAB21455.1:C186S and applied mutation signature C186S to reconstruct the assayed variant sequence.


organism: Escherichia coli

substrate_name: Arc-MYL-st11 plus

assay_conditions_summary: pH 7.6; 310.15 K; cofactor ATP

proof_lines:
pH 7.6, 37°C, HslUV
pH 8, 37°C

substrate: Arc-MYL-st11 plus


proof_lines: deletion mutant DELTA175-209linker, pH 7.6, 37°C


organism: Haemophilus influenzae

substrate_name: SulA

proof_lines: pH 5.5, 37°C, wild-type SulA, recombinant enzyme

substrate: SulA


substrate_name: SulA mutant P8L

substrate: SulA mutant P8L


enzyme_display_name: MMP alpha-(1->4)-mannosyltransferase

organism: Mycolicibacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 / 3849)

substrate_name: sMetMan(4) (at 37 degrees Celsius)

proof_lines: kcat is 0.047 sec(-1) with sMetMan(4) as substrate (at 37 degrees Celsius). kcat is 0.025 sec(-1) with sMan(4) as substrate (at 37 degrees Celsius). kcat is 0.048 sec(-1) with GDP-mannose as substrate (in the presence of sMetMan(4), at 37 d

enzyme: MMP alpha-(1->4)-mannosyltransferase

substrate: sMetMan(4) (at 37 degrees Celsius)


organism: Mycolicibacterium hassiacum

sequence_variant_note: Resolved canonical sequence from UniProtKB:K5BE02 REST lookup on 2026-07-08 plus E313A reconstruction; RCSB 7QSG SIFTS maps the structure entity to K5BE02. and applied mutation signature E313A to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme E313A, with propyl-alpha-D-mannopyranosyl-(1->4)-3-O-methyl-alpha-D-mannopyranosyl-(1->4)-3-O-methyl-alpha-D-mannopyranoside as cosubstrate, at pH 8.5 and 37Â°C

proof_lines: mutant enzyme E313A, at pH 8.5 and 37Â°C


substrate_name: GDP-alpha-D-mannose; sMetMan4; sMan4

sequence_source: UniProtKB:K5BE02 REST lookup on 2026-07-08; RCSB 7QSG SIFTS maps the structure entity to K5BE02.

proof_lines: wild type enzyme, with propyl-alpha-D-mannopyranosyl-(1->4)-alpha-D-mannopyranosyl-(1->4)-alpha-D-mannopyranosyl-(1->4)-alpha-D-mannopyranoside as cosubstrate, at pH 8.5 and 45Â°C

proof_lines: wild type enzyme, with propyl-alpha-D-mannopyranosyl-(1->4)-3-O-methyl-alpha-D-mannopyranosyl-(1->4)-3-O-methyl-alpha-D-mannopyranoside as cosubstrate, at pH 8.5 and 45Â°C

substrate: GDP-alpha-D-mannose; sMetMan4; sMan4


organism: Mycolicibacterium smegmatis

proof_lines: at 37°C, pH not specified in the publication


enzyme_display_name: tyrosine--arginine ligase

organism: Homo sapiens

substrate_name: L-tyrosine

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

proof_lines: pH and temperature not specified in the publication

proof_lines: pH 7.4, 37°C, recombinant His-tagged enzyme

enzyme: tyrosine--arginine ligase

substrate: L-tyrosine


organism: Rattus norvegicus

substrate_name: ATP; L-Arginine; L-Tyrosine

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP

proof_lines:
37°C, pH 8.0, enzyme from adrenal gland
37°C, pH 8.0, enzyme from spinal cord
37°C, pH 8.0

substrate: ATP; L-Arginine; L-Tyrosine


enzyme_display_name: glucosyl-3-phosphoglycerate phosphatase

organism: Persephonella marina

enzyme: glucosyl-3-phosphoglycerate phosphatase


organism: Thermus thermophilus


enzyme_display_name: neopullulanase

organism: uncultured microorganism

substrate_name: pullulan

proof_lines: optimal pH 7.0, optimal temperature 75°C

enzyme: neopullulanase

substrate: pullulan


enzyme_display_name: phosphomevalonate decarboxylase

organism: Anaerolinea thermophila

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

enzyme: phosphomevalonate decarboxylase


organism: Haloferax volcanii

substrate_name: mevalonate 5-phosphate

substrate: mevalonate 5-phosphate


organism: Methanococcoides burtonii


organism: Geobacillus stearothermophilus


organism: Ruminococcus bromii


organism: Thermoanaerobacter ethanolicus


enzyme_display_name: deuterolysin

organism: Aspergillus oryzae

proof_lines: pH 9.0, 30°C, Co-substituted deuterolysin

enzyme: deuterolysin


organism: Bacillus sp

substrate_name: alpha-cyclodextrin (at pH 7.0 and 50 degrees Celsius)

proof_lines: kcat is 78.18 sec(-1) with beta-cyclodextrin as substrate. kcat is 22.11 sec(-1) with amylose as substrate. kcat is 50.36 sec(-1) with amylopectin as substrate (PubMed:16536613). kcat is 101 min(-1) for dimeric form of the enzyme with beta-


organism: Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)


enzyme_display_name: glutamate--prephenate aminotransferase

organism: Sorghum bicolor x Sorghum sudanesis

substrate_name: prephenate

assay_conditions_summary: pH 8

enzyme: glutamate--prephenate aminotransferase

substrate: prephenate


organism: Arabidopsis thaliana

substrate_name: L-glutamate

substrate: L-glutamate


organism: Sinorhizobium meliloti


organism: Streptomyces avermitilis

proof_lines: cosubstrate N-succinyl-L-2,6-diaminoheptanedioate, pH 8.0, 30°C


organism: Synechocystis sp.


substrate_name: aspartate for the asparte aminotransferase activity


organism: Petunia hybrida

substrate_name: prephenate (with 20 mM aspartate as cosubstrate)


organism: Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)

substrate_name: GPG (at 30 degrees Celsius)

substrate: GPG (at 30 degrees Celsius)


organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

substrate_name: glucosyl-3-phosphoglycerate (at 37 degrees Celsius)


organism: Persephonella marina (strain DSM 14350 / EX-H1)

substrate_name: GPG (at 70 degrees Celsius)

substrate: GPG (at 70 degrees Celsius)


enzyme_display_name: anthocyanin 6''-O-malonyltransferase

organism: Pericallis cruenta

substrate_name: malonyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: anthocyanin 6''-O-malonyltransferase

substrate: malonyl-CoA


enzyme_display_name: glutaredoxin-dependent peroxiredoxin

organism: Mus musculus

substrate_name: glutaredoxin 2

proof_lines: pH 7.0, temperature not specified in the publication

enzyme: glutaredoxin-dependent peroxiredoxin

substrate: glutaredoxin 2


organism: Thermotoga maritima

assay_conditions_summary: cofactor NADH


enzyme_display_name: dissimilatory sulfite reductase

organism: Archaeoglobus fulgidus

substrate_name: sulfite

proof_lines: in the presence of DsrC, at pH 7.0 and 60Â°C

proof_lines: in the presence of DsrD, at pH 7.0 and 60Â°C

proof_lines: in the absence of DsrD, at pH 7.0 and 60Â°C

enzyme: dissimilatory sulfite reductase

substrate: sulfite


substrate_name: Unknown

substrate: Unknown


organism: Populus trichocarpa

substrate_name: phosphatidylcholine hydroperoxide

substrate: phosphatidylcholine hydroperoxide


organism: Dahlia pinnata


organism: Nitratidesulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / CCUG 34227 / NCIMB 8303 / VKM B-1760 / Hildenborough)

proof_lines: kcat is 0.31 sec(-1) for sulfite. kcat is 0.038 sec(-1) for nitrite.


organism: Cereibacter sphaeroides (strain ATCC 17029 / ATH 2.4.9)

substrate_name: oxaloacetate

proof_lines: kcat is 150 sec(-1) with oxaloacetate as substrate. kcat is 56 sec(-1) with prephenate as substrate.

substrate: oxaloacetate


organism: Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)

proof_lines: kcat is 33 sec(-1) with oxaloacetate as substrate. kcat is 0.8 sec(-1) with prephenate as substrate.


organism: Rhizobium meliloti (strain 1021)

proof_lines: kcat is 205 sec(-1) with oxaloacetate as substrate. kcat is 43 sec(-1) with prephenate as substrate.


organism: Bacillus sp

substrate: alpha-cyclodextrin (at pH 7.0 and 50 degrees Celsius)


organism: Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)


organism: Chrysanthemum x morifolium

substrate_name: cyanidin 3-O-beta-D-glucoside

substrate: cyanidin 3-O-beta-D-glucoside


assay_conditions_summary: pH 7; 303.15 K; cofactor CoA

proof_lines: pH 7.0, 30°C, value is obtained by measurement of formation of pelargonidin 3-O-(6-O-malonyl-beta-D-glucoside)


organism: Populus x canadensis

substrate_name: tert-butyl hydroperoxide

substrate: tert-butyl hydroperoxide


organism: Allochromatium vinosum

assay_conditions_summary: pH 7; 303.15 K; cofactor NADH

proof_lines: with methyl viologen as cosubstrate, at pH 7.0 and 30°C

proof_lines: with NADH as cosubstrate, at pH 7.0 and 30°C


organism: Desulfocurvibacter africanus

assay_conditions_summary: pH 6

proof_lines: pH 6.0, temperature not specified in the publication


organism: Nitratidesulfovibrio vulgaris

substrate_name: hydroxylamine

substrate: hydroxylamine


organism: Nicotiana tabacum


organism: Solanum tuberosum

substrate_name: 16-hydroxypalmitic acid

substrate: 16-hydroxypalmitic acid


organism: Arabidopsis thaliana

substrate_name: feruloyl-CoA

proof_lines: 16-hydroxypalmitic acid is the main acyl acceptor.

substrate: feruloyl-CoA


enzyme_display_name: dolichol kinase

organism: Ascaris suum

substrate_name: CTP

enzyme: dolichol kinase

substrate: CTP


organism: Bos taurus

proof_lines: pH 7.5, 35°C, in Triton X-100/dimyristoylphosphatidylcholine 1:1

proof_lines: pH 7.5, 35°C, in deoxycholate/dimyristoylphosphatidylcholine 1:1


organism: Glycine max

substrate_name: dolichol

substrate: dolichol


organism: Onchocerca volvulus


organism: Rattus norvegicus

proof_lines:
pH 7.4, 37°C
pH 7.4, 37°C, skeletal muscle enzyme
pH 7.4, 37°C, brain enzyme


organism: Tetrahymena pyriformis


organism: Homo sapiens


enzyme_display_name: sulfate adenylyltransferase (ADP)

substrate_name: adenosine 5'-phosphosulfate

assay_conditions_summary: pH 6.8; 303.15 K; buffer 0.1 M HEPES/KOH

proof_lines: in 0.1 M HEPES/KOH buffer (pH 6.8), 0.1 M potassium phosphate (pH 6.8), 0.1 M KH2PO4 (pH 4.2), at 30°C

enzyme: sulfate adenylyltransferase (ADP)

substrate: adenosine 5'-phosphosulfate


organism: Thiobacillus denitrificans


enzyme_display_name: ATP adenylyltransferase

organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

sequence_source: Selected source_record sequence for UniProtKB P22108; direct UniProtKB FASTA check matched exactly on 2026-07-08

proof_lines: kcat is 93.8 sec(-1) with Ap4A as substrate.

enzyme: ATP adenylyltransferase


sequence_source: Selected source_record sequence for UniProtKB P16550; direct UniProtKB FASTA check matched exactly on 2026-07-08

proof_lines: kcat is 81.2 sec(-1) with Ap4A as substrate.


organism: Caenorhabditis elegans

assay_conditions_summary: pH 6.8; 303.15 K; buffer 0.1 M HEPES/KOH; cofactor ADP


organism: Prosthecochloris vibrioformis

proof_lines:
pH 8.0, 50 mM phosphate
pH 8, 1 mM adenosine 5-phosphosulfate


organism: Saccharomyces cerevisiae

assay_conditions_summary: pH 8; 303.15 K; cofactor ATP

proof_lines: 4 mM adenosine 5-phosphosulfate, 30°C, pH 8


organism: Saccharomyces fragilis

substrate_name: ADP

assay_conditions_summary: 303.15 K; cofactor ADP

substrate: ADP


enzyme_display_name: phenol 2-monooxygenase (FADH2)

organism: Rhodococcus erythropolis

substrate_name: FAD

assay_conditions_summary: cofactor NADPH

proof_lines:
with FMN as electron acceptor
with FAD as electron acceptor

enzyme: phenol 2-monooxygenase (FADH2)

substrate: FAD


enzyme_display_name: spleen exonuclease

organism: Plasmodium falciparum

mutation_signature: C-terminal truncation mutant

mutation_type: truncation

enzyme: spleen exonuclease


organism: Plasmodium yoelii


enzyme_display_name: adenosine nucleosidase

organism: Hordeum vulgare

substrate_name: adenosine

assay_conditions_summary: pH 5.4; buffer MES

enzyme: adenosine nucleosidase

substrate: adenosine


organism: Lathyrus oleraceus

substrate_name: Inosine

substrate: Inosine


organism: Lupinus luteus


organism: Solanum lycopersicum

proof_lines:
form R1
form R2
from Lf


organism: Triticum aestivum


organism: Parageobacillus thermoglucosidasius

substrate_name: riboflavin

substrate: riboflavin


assay_conditions_summary: pH 7; 298.15 K; cofactor FAD


assay_conditions_summary: pH 7; 298.15 K; cofactor NADH

proof_lines: NADH-dependent reduction, 25°C, pH 7.0, recombinant PheA2


substrate_name: FMN

substrate: FMN


organism: Auxenochlorella pyrenoidosa

substrate_name: phenol

substrate: phenol


organism: Bos taurus

substrate_name: thymidine 3'-(4-nitrophenyl)phosphate

substrate: thymidine 3'-(4-nitrophenyl)phosphate


organism: Cavia porcellus


organism: Homo sapiens


enzyme_display_name: galactosylceramidase

organism: Aspergillus oryzae

substrate_name: Unknown

proof_lines: pH 4.6, temperature not specified in the publication

enzyme: galactosylceramidase

substrate: Unknown


proof_lines: taurocholate and oleic acid activated, in leukocytes


substrate_name: galactosylceramide

substrate: galactosylceramide


organism: Mus musculus


organism: Arabidopsis thaliana

proof_lines: recombinant enzyme expressed in Escherichia coli


organism: Brassica oleracea


organism: Coffea arabica


organism: Helianthus tuberosus


substrate_name: glucosylceramide (at 37 degrees Celsius and pH 6.0)

proof_lines: kcat is 0.43 min(-1) for the hydrolysis of glucosylceramide (PubMed:17595169). kcat<0.01 min(-1) for the hydrolysis of galactosylceramide (PubMed:17595169). kcat is 7.26 min(-1) for the hydrolysis of C6-NBD-glucosylceramide (PubMed:17595169


proof_lines: kcat is 2500 min(-1) for the hydrolysis of beta-D-glucosyl-(1->3)-O-lithocholate (PubMed:9111029). kcat is 1300 min(-1) for the hydrolysis of beta-D-glucosyl-(1->3)-O-chenodeoxycholate (PubMed:9111029). kcat is 4700 min(-1) for the hydrolys


substrate_name: uridine

proof_lines: kcat is 2.65 sec(-1) with xanthosine as substrate (PubMed:30787180). kcat is 33.5 sec(-1) with uridine as substrate (PubMed:30787180). kcat is 23.3 sec(-1) with xanthosine as substrate (when in complex with URH2) (PubMed:30787180). kcat is

substrate: uridine


organism: Caenorhabditis elegans

substrate_name: inosine (at pH 7.0 and 37 degrees Celsius)

assay_conditions_summary: pH 7

proof_lines: kcat is 0.79 sec(-1) with inosine as substrate (at pH 7.0 and 37 degrees Celsius) (PubMed:12777783). kcat is 0.203 sec(-1) with inosine as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:28218438). kcat is 0.78 sec(-1) with adenosine a


substrate_name: 5'-OH ApA diribonucleotide

substrate: 5'-OH ApA diribonucleotide


substrate_name: N-acyl-beta-D-galactosylsphingosine

substrate: N-acyl-beta-D-galactosylsphingosine


substrate_name: inosine

substrate: inosine


enzyme_display_name: beta-lactamase

organism: Serratia marcescens

substrate_name: H2O; Ampicillin

enzyme: beta-lactamase

substrate: H2O; Ampicillin


organism: Escherichia coli


organism: Klebsiella pneumoniae

substrate_name: Nitrocefin; H2O

substrate: Nitrocefin; H2O


organism: Pseudomonas aeruginosa

substrate_name: H2O; Penicillin G

substrate: H2O; Penicillin G


organism: Aeromonas hydrophila

substrate_name: H2O; Imipenem

substrate: H2O; Imipenem


organism: Bacillus licheniformis

substrate_name: H2O; Phenoxymethylpenicillin

substrate: H2O; Phenoxymethylpenicillin


organism: Enterobacter cloacae

substrate_name: Cefotaxime; H2O

substrate: Cefotaxime; H2O


substrate_name: H2O; Nitrocefin

substrate: H2O; Nitrocefin


source_db: skid

source_license: CC BY-NC-ND 4.0

proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=30°C, pH=7
Raw SKiD Env: Temp=25°C, pH=7.5

source: skid


substrate_name: nitrocefin

substrate: nitrocefin


substrate_name: cefepime

proof_lines:
Raw SKiD Env: Temp=-----, pH=7.4
Raw SKiD Env: Temp=-----, pH=7.2
Raw SKiD Env: Temp=25°C, pH=7.5

substrate: cefepime


substrate_name: ceftazidime

substrate: ceftazidime


substrate_name: ticarcillin

substrate: ticarcillin


substrate_name: Imipenem

substrate: Imipenem


proof_lines:
Raw SKiD Env: Temp=25°C, pH=7.5
Raw SKiD Env: Temp=-----, pH=-----


enzyme_display_name: carbonic anhydrase

organism: Candida albicans

substrate_name: H2O; CO2

enzyme: carbonic anhydrase

substrate: H2O; CO2


organism: Cryptococcus neoformans


organism: Homo sapiens

substrate_name: CO2; H2O

substrate: CO2; H2O


organism: Spinacia oleracea


enzyme_display_name: beta-glucosidase

organism: Prunus dulcis

substrate_name: beta-Methyl-D-glucopyranoside; H2O

enzyme: beta-glucosidase

substrate: beta-Methyl-D-glucopyranoside; H2O


organism: Oryza sativa

substrate_name: H2O; p-Nitrophenyl-beta-D-glucoside

substrate: H2O; p-Nitrophenyl-beta-D-glucoside


organism: Pyrococcus furiosus


enzyme_display_name: homomethionine N-monooxygenase

organism: Arabidopsis thaliana

substrate_name: Dihomomethionine; O2

proof_lines: pH and temperature not specified in the publication

enzyme: homomethionine N-monooxygenase

substrate: Dihomomethionine; O2


substrate_name: Hexahomomethionine; O2

substrate: Hexahomomethionine; O2


substrate_name: pseudouridine1915 in 23S rRNA

substrate: pseudouridine1915 in 23S rRNA


substrate_name: S-Adenosyl-L-methionine; Pseudouridine

substrate: S-Adenosyl-L-methionine; Pseudouridine


enzyme_display_name: dimethylsulfoniopropionate demethylase

organism: Candidatus Pelagibacter communis

proof_lines: pH 7.5, temperature not specified in the publication

enzyme: dimethylsulfoniopropionate demethylase


organism: Candidatus Pelagibacter ubique

proof_lines:
Raw SKiD Env: Temp=-----, pH=7.5
Raw SKiD Env: Temp=30°C, pH=7.5


organism: Ruegeria pomeroyi

substrate_name: dimethylsulfoniopropionate

proof_lines: in cell extracts, similar to that of purified recombinant DmdA

substrate: dimethylsulfoniopropionate


enzyme_display_name: levansucrase

organism: Bacillus amyloliquefaciens

substrate_name: sucrose

proof_lines: recombinant Lev-(GGS)6-D272NInu fusion enzyme, pH 5.0, 50°C

proof_lines: recombinant D86NLev-(GGS)6-Inu fusion enzyme, pH 5.0, 50°C

proof_lines: recombinant Lev-(GGS)6-Inu fusion enzyme, pH 5.0, 50°C

enzyme: levansucrase

substrate: sucrose


proof_lines: recombinant wild-type enzyme Lev, pH 5.0, 50°C


proof_lines: mutant K82H/N83R, Hill model, Hill coefficient 1.18, hydrolysis of sucrose, pH 6, 50°C

proof_lines: mutant K82H/N83R, transfructosylation reaction, hydrolysis of sucrose, pH 6, 50°C

proof_lines: mutant K82H/N83R, Hill model, Hill coefficient 0.57, total reaction, pH 6, 50°C


proof_lines: wild-type, Michaelis-Menten model, total reaction, total reaction, pH 6, 50°C

proof_lines: wild-type, Hill model, Hill coefficient 0.59, total reaction, pH 6, 50°C

proof_lines: wild-type, transfructosylation reaction, pH 6, 50°C


organism: Bacillus subtilis

proof_lines: mutant enzyme Y429N, 0.5 U/ml of enzyme activity at 37°C and pH 6.0


substrate_name: Sucrose

substrate: Sucrose


substrate_name: Sucrose; (2,6-beta-D-Fructosyl)n

substrate: Sucrose; (2,6-beta-D-Fructosyl)n


proof_lines: mutant enzyme G361F, 0.5 U/ml of enzyme activity at 37°C and pH 6.0


proof_lines: mutant enzyme R360S, 0.5 U/ml of enzyme activity at 37°C and pH 6.0


proof_lines: mutant enzyme I341V, 0.5 U/ml of enzyme activity at 37°C and pH 6.0


proof_lines: mutant enzyme H243L, 0.5 U/ml of enzyme activity at 37°C and pH 6.0


proof_lines: mutant S164A, hydrolysis of sucrose, pH 6, 37°C

proof_lines: mutant S164A, hydrolysis of sucrose, cosubstrate levan , 5 g/l, pH 6, 37°C

proof_lines: mutant S164A, transfer reaction, cosubstrate levan , 5 g/l, pH 6, 37°C


proof_lines: wild-type, transfer reaction, cosubstrate levan, 5 g/l, pH 6, 37°C

proof_lines: wild-type, transfer reaction, pH 6, 37°C

proof_lines: wild-type, hydrolysis of sucrose, cosubstrate levan, 5 g/l, pH 6, 37°C


organism: Brenneria sp. EniD312


organism: Beijerinckia indica subsp. indica


proof_lines: transfructosylating activity, at pH 6.0 and 37°C


proof_lines: mutant enzyme R369K, 0.5 U/ml of enzyme activity at 37°C and pH 6.0


proof_lines: mutant enzyme R433A, 0.5 U/ml of enzyme activity at 37°C and pH 6.0


organism: Erwinia tasmaniensis

proof_lines:
at pH 6.5 and 37°C
Raw SKiD Env: Temp=37°C, pH=6.5


organism: Gluconacetobacter diazotrophicus

proof_lines:
wild-type enzyme
30°C, pH 5.0, wild-type enzyme


substrate_name: H2O; Sucrose

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=30°C, pH=5

substrate: H2O; Sucrose


organism: Gluconobacter oxydans


organism: Leuconostoc mesenteroides


organism: Novosphingobium aromaticivorans


organism: Paraburkholderia graminis


organism: Priestia megaterium

proof_lines:
wild-type, pH 6.6, 25°C
wild-type, pH 6.6, 37°C


proof_lines: variant carrying Lactobacillus johnsonii loop2, pH 6.6, 25°C

proof_lines: variant carrying Lactobacillus johnsonii loop3-4, pH 6.6, 37°C

proof_lines: variant carrying Lactobacillus johnsonii loop3-7, pH 6.6, 37°C


organism: Pseudomonas chlororaphis subsp. aurantiaca

proof_lines: inhibition of sucrose hydrolysis, pH 6.0-6.6, 60°C


organism: Pseudomonas syringae


organism: Pseudomonas syringae pv. tomato

proof_lines: inhibition of sucrose hydrolysis, pH 6.0, 60°C


organism: Streptococcus salivarius

substrate_name: D-Glucose

substrate: D-Glucose


substrate_name: D-Fructose

substrate: D-Fructose


organism: Vibrio natriegens


organism: Zymomonas mobilis

proof_lines:
levan synthesis activity
transfructosylation activity
total activity


enzyme_display_name: pyruvate kinase

organism: Rattus norvegicus

substrate_name: Phosphoenolpyruvate; ADP

assay_conditions_summary: cofactor ATP

enzyme: pyruvate kinase

substrate: Phosphoenolpyruvate; ADP


organism: Saccharomyces cerevisiae

assay_conditions_summary: cofactor ADP


enzyme_display_name: N-acetyllactosaminidase

organism: Aquimarina sp. TRL1

proof_lines: 35°C, pH not specified in the publication

enzyme: N-acetyllactosaminidase


organism: Paludibaculum fermentans


organism: Parabacteroides distasonis


organism: Planctomycetales bacterium


organism: Treponema denticola


enzyme_display_name: fumarylacetoacetase

organism: Homo sapiens

enzyme: fumarylacetoacetase


organism: Escherichia coli

substrate_name: RlmH; S-Adenosyl-L-methionine

substrate: RlmH; S-Adenosyl-L-methionine


organism: Candidatus Pelagibacter communis

proof_lines: noncompetitive inhibition, product inhibition


organism: Actinomyces viscosus


organism: Aspergillus tamarii

proof_lines: transfructosylation reaction, pH 7.4, 50°C

proof_lines: application of a static magnetic field with a 1450 G magnetic flux density prior to assay, pH 5, 55°C


organism: Bacillus amyloliquefaciens


organism: Bacillus licheniformis

proof_lines: mutant enzyme N251W, total activity, at pH 6.0 and 50°C

proof_lines: mutant enzyme N251W, hydrolytic activity, at pH 6.0 and 50°C

proof_lines: mutant enzyme N251W, transfructosylation activity, at pH 6.0 and 50°C


proof_lines: wild type enzyme, total activity, at pH 6.0 and 50°C

proof_lines: wild type enzyme, hydrolytic activity, at pH 6.0 and 50°C

proof_lines: wild type enzyme, transfructosylation activity, at pH 6.0 and 50°C


proof_lines: mutant enzyme K372A, hydrolytic activity, at pH 6.0 and 50°C

proof_lines: mutant enzyme K372A, total activity, at pH 6.0 and 50°C

proof_lines: mutant enzyme K372A, transfructosylation activity, at pH 6.0 and 50°C


proof_lines: mutant enzyme N251W/K372Y, total activity, at pH 6.0 and 50°C

proof_lines: mutant enzyme N251W/K372Y, transfructosylation activity, at pH 6.0 and 50°C


proof_lines: mutant enzyme K372Y, total activity, at pH 6.0 and 50°C

proof_lines: mutant enzyme K372Y, hydrolytic activity, at pH 6.0 and 50°C

proof_lines: mutant enzyme K372Y, transfructosylation activity, at pH 6.0 and 50°C


organism: Bacillus subtilis

proof_lines: levansucrase-endolevanase fusion enzyme, pH and temperature not specified in the publication

proof_lines: levansucrase, pH and temperature not specified in the publication


organism: Rhodotorula diobovata


organism: Pelagibacter ubique (strain HTCC1062)

substrate_name: tetrahydrafolate

proof_lines: Upon expression and purification from E.coli.

substrate: tetrahydrafolate


organism: Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)


organism: Bacillus subtilis (strain 168)

proof_lines: kcat is 164.6 sec(-1) with sucrose as substrate (PubMed:18596022). kcat is 236.7 sec(-1) with sucrose as substrate (PubMed:33303628). kcat is 71.2 sec(-1) with sucrose as substrate (for hydrolysis, in the absence of levan F4). kcat is 69.8


organism: Gluconacetobacter diazotrophicus


organism: Pseudomonas syringae pv. actinidiae

substrate_name: sucrose (at pH 5.0)

assay_conditions_summary: pH 5

proof_lines: kcat is 7300 min(-1) with sucrose as substrate (at pH 5.0). kcat is 7500 min(-1) with sucrose as substrate (at pH 7.0).

substrate: sucrose (at pH 5.0)


proof_lines: kcat is 8500 min(-1) with sucrose as substrate (at pH 5.0). kcat is 15000 min(-1) with sucrose as substrate (at pH 7.0).


organism: Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)

proof_lines: kcat is 1700 min(-1) for sucrose hydrolase activity.


organism: Aquimarina sp. (strain TRL1)

substrate_name: pNP-LNB

proof_lines: kcat is 2.16 sec(-1) with pNP-LNB as substrate (PubMed:39239753). kcat is 3.9 sec(-1) with pNP-LacNAc as substrate (PubMed:39239753).

substrate: pNP-LNB


organism: Paludibaculum fermentans

substrate_name: pNP-LacNAc

verification_status: verified

assay_conditions_summary: pH 6; 318.15 K; buffer pH 6

column_label: Table 6 (Kinetic parameters k cat, K M, K i and k cat/K M of AquiGH20, PadiGH20, PafeGH20, PlbaGH20 and TrdeGH20, determined at their respective optimal conditions of pH and temperature using pNP-LNB and pNP-LacNAc as substrates), enzyme row 'PafeGH20'. pNP-LacNAc column group: column 'k cat' = 17.1, column 'K M' = 1408, column 'K i' = 736, column 'k cat/K M' = 1.30 x 10^4. pNP-LNB column group of the same row: column 'k cat' = en dash, column 'K M' = en dash, column 'k cat/K M' = 6.66 x 10^2.

normalized_value_unit_evidence: kcat: printed Table 6 cell 17.1 s-1; the printed unit is already s^-1, so no conversion is applied and the exported value is 17.1 s^(-1). Km: printed Table 6 cell 1408 uM; 1408 / 1000 = 1.408, so the exported value is 1.408 mM. kcat/Km: printed Table 6 cell 1.30 x 10^4 s-1 M-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1.30 x 10^4 x 0.001 = 13.0 s^-1 mM^-1; the export row stores no kcat_over_km value, so this converted figure is kept as typed provenance only and no ratio metric is written.

raw_value_unit_evidence: Table 6, enzyme row 'PafeGH20', pNP-LacNAc column group: k cat = 17.1 s-1; K M = 1408 uM; K i = 736 uM; printed k cat/K M = 1.30 x 10^4 s-1 M-1. The pNP-LNB column group of the same row prints an en dash for k cat and for K M and 6.66 x 10^2 s-1 M-1 for k cat/K M.

row_label: Table 6, enzyme row 'PafeGH20', pNP-LacNAc column group (k cat, K M, K i, k cat/K M)

table_label: Table 6: Kinetic parameters k cat (s-1), K M (uM), K i (uM), and k cat/K M (s-1 M-1) of AquiGH20, PadiGH20, PafeGH20, PlbaGH20, and TrdeGH20 (Chembiochem 2025, 26(2), e202400710)

substrate: pNP-LacNAc

status: verified


organism: Parabacteroides distasonis

proof_lines: kcat is 14.6 sec(-1) with pNP-LNB as substrate (PubMed:39239753). kcat is 23.6 sec(-1) with pNP-LacNAc as substrate (PubMed:39239753).