column_label: Table III (journal page 17888), substrate row `D-Threitol (footnote a)`, Human column group: Km (mM) = 18; kcat (s^-1) = 0.48; kcat/Km (s^-1 mM^-1) = 0.026.

normalized_value_unit_evidence: kcat: printed value 0.48 s^-1 -> 0.48 s^(-1); the catalog holds 0.48 s^(-1); no unit conversion applied (the paper already prints s^-1). Km: printed value 18 mM -> 18.0 mM; the catalog holds 18.0 mM; no unit conversion applied (the paper already prints mM). kcat/Km: The catalog kcat_over_km 0.026666666666666665 s^(-1)*mM^(-1) is NOT a printed cell: it is the exact full-precision quotient of this row's own printed kcat and Km, 0.48 s^-1 / 18 mM. Table III does print a rounded kcat/Km cell for this row and species, `0.026` s^-1 mM^-1, which is stated here only for comparison; the catalog number itself remains derived, not quoted, so this pass types the ratio as derived_same_measurement and leaves the paper ratio null.

row_label: Table III, substrate row `D-Threitol (footnote a)`, Human column group


enzyme_display_name: L-xylulose reductase

organism: Mesocricetus auratus

substrate_name: Threitol

source_db: oed

verification_status: verified

ki_display: Not reported

mutation_signature: Unknown

protein_accession_database: UniProt

kcat_over_km_origin: calculated

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

smiles: OCC(O)C(O)CO

assay_conditions_summary: pH 7; 298.15 K; buffer 0.1 M potassium phosphate; cofactor NADP+

column_label: Table III (journal page 17888), substrate row `D-Threitol (footnote a)`, Hamster column group: Km (mM) = 34; kcat (s^-1) = 16; kcat/Km (s^-1 mM^-1) = 0.47.

normalized_value_unit_evidence: kcat: printed value 16 s^-1 -> 16.0 s^(-1); the catalog holds 16.0 s^(-1); no unit conversion applied (the paper already prints s^-1). Km: printed value 34 mM -> 34.0 mM; the catalog holds 34.0 mM; no unit conversion applied (the paper already prints mM). kcat/Km: The catalog kcat_over_km 0.47058823529411764 s^(-1)*mM^(-1) is NOT a printed cell: it is the exact full-precision quotient of this row's own printed kcat and Km, 16 s^-1 / 34 mM. Table III does print a rounded kcat/Km cell for this row and species, `0.47` s^-1 mM^-1, which is stated here only for comparison; the catalog number itself remains derived, not quoted, so this pass types the ratio as derived_same_measurement and leaves the paper ratio null.

row_label: Table III, substrate row `D-Threitol (footnote a)`, Hamster column group

table_label: Table III Substrate specificity for monosaccharides in the reduction and dehydrogenation reactions catalyzed by rodent and human DCXRs at pH 7.0

data_origin: experimental

enzyme: L-xylulose reductase

substrate: Threitol

status: verified

source: oed


organism: Mus musculus

column_label: Table III (journal page 17888), substrate row `D-Threitol (footnote a)`, Mouse column group: Km (mM) = 37; kcat (s^-1) = 16; kcat/Km (s^-1 mM^-1) = 0.43.

normalized_value_unit_evidence: kcat: printed value 16 s^-1 -> 16.0 s^(-1); the catalog holds 16.0 s^(-1); no unit conversion applied (the paper already prints s^-1). Km: printed value 37 mM -> 37.0 mM; the catalog holds 37.0 mM; no unit conversion applied (the paper already prints mM). kcat/Km: The catalog kcat_over_km 0.43243243243243246 s^(-1)*mM^(-1) is NOT a printed cell: it is the exact full-precision quotient of this row's own printed kcat and Km, 16 s^-1 / 37 mM. Table III does print a rounded kcat/Km cell for this row and species, `0.43` s^-1 mM^-1, which is stated here only for comparison; the catalog number itself remains derived, not quoted, so this pass types the ratio as derived_same_measurement and leaves the paper ratio null.

row_label: Table III, substrate row `D-Threitol (footnote a)`, Mouse column group


organism: Rattus norvegicus

column_label: Table III (journal page 17888), substrate row `D-Threitol (footnote a)`, Rat column group: Km (mM) = 16; kcat (s^-1) = 12; kcat/Km (s^-1 mM^-1) = 0.75.

normalized_value_unit_evidence: kcat: printed value 12 s^-1 -> 12.0 s^(-1); the catalog holds 12.0 s^(-1); no unit conversion applied (the paper already prints s^-1). Km: printed value 16 mM -> 16.0 mM; the catalog holds 16.0 mM; no unit conversion applied (the paper already prints mM). kcat/Km: kcat/Km: printed cell 0.75 s^-1 mM^-1 -> 0.75 s^(-1)*mM^(-1); the catalog holds 0.75 s^(-1)*mM^(-1); no unit conversion applied (the paper already prints s^-1 mM^-1). The printed cell is also the exact quotient of this row's own printed kcat and Km, 12 s^-1 / 16 mM.

row_label: Table III, substrate row `D-Threitol (footnote a)`, Rat column group


substrate_name: xylitol

verification_status: unverified

sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature S136A to reconstruct the assayed variant sequence.

mutation_type: substitution

substrate: xylitol

status: unverified


column_label: Table III (journal page 17888), substrate row `Xylitol (footnote a)`, Rat column group: Km (mM) = 11; kcat (s^-1) = 10; kcat/Km (s^-1 mM^-1) = 0.91.

normalized_value_unit_evidence: kcat: printed value 10 s^-1 -> 10.0 s^(-1); the catalog holds 10.0 s^(-1); no unit conversion applied (the paper already prints s^-1). Km: printed value 11 mM -> 11.0 mM; the catalog holds 11.0 mM; no unit conversion applied (the paper already prints mM). kcat/Km: The catalog kcat_over_km 0.9090909090909091 s^(-1)*mM^(-1) is NOT a printed cell: it is the exact full-precision quotient of this row's own printed kcat and Km, 10 s^-1 / 11 mM. Table III does print a rounded kcat/Km cell for this row and species, `0.91` s^-1 mM^-1, which is stated here only for comparison; the catalog number itself remains derived, not quoted, so this pass types the ratio as derived_same_measurement and leaves the paper ratio null.

row_label: Table III, substrate row `Xylitol (footnote a)`, Rat column group


organism: Cavia porcellus

column_label: Table III (journal page 17888), substrate row `Xylitol (footnote a)`, Guinea pig column group: Km (mM) = 10; kcat (s^-1) = 20; kcat/Km (s^-1 mM^-1) = 2.0.

normalized_value_unit_evidence: kcat: printed value 20 s^-1 -> 20.0 s^(-1); the catalog holds 20.0 s^(-1); no unit conversion applied (the paper already prints s^-1). Km: printed value 10 mM -> 10.0 mM; the catalog holds 10.0 mM; no unit conversion applied (the paper already prints mM). kcat/Km: kcat/Km: printed cell 2.0 s^-1 mM^-1 -> 2.0 s^(-1)*mM^(-1); the catalog holds 2.0 s^(-1)*mM^(-1); no unit conversion applied (the paper already prints s^-1 mM^-1). The printed cell is also the exact quotient of this row's own printed kcat and Km, 20 s^-1 / 10 mM.

row_label: Table III, substrate row `Xylitol (footnote a)`, Guinea pig column group


organism: Homo sapiens

column_label: Table III (journal page 17888), substrate row `Xylitol (footnote a)`, Human column group: Km (mM) = 25; kcat (s^-1) = 0.60; kcat/Km (s^-1 mM^-1) = 0.024.

normalized_value_unit_evidence: kcat: printed value 0.60 s^-1 -> 0.6 s^(-1); the catalog holds 0.6 s^(-1); no unit conversion applied (the paper already prints s^-1). Km: printed value 25 mM -> 25.0 mM; the catalog holds 25.0 mM; no unit conversion applied (the paper already prints mM). kcat/Km: kcat/Km: printed cell 0.024 s^-1 mM^-1 -> 0.024 s^(-1)*mM^(-1); the catalog holds 0.024 s^(-1)*mM^(-1); no unit conversion applied (the paper already prints s^-1 mM^-1). The printed cell is also the exact quotient of this row's own printed kcat and Km, 0.60 s^-1 / 25 mM.

row_label: Table III, substrate row `Xylitol (footnote a)`, Human column group


column_label: Table III (journal page 17888), substrate row `Xylitol (footnote a)`, Hamster column group: Km (mM) = 40; kcat (s^-1) = 28; kcat/Km (s^-1 mM^-1) = 0.70.

normalized_value_unit_evidence: kcat: printed value 28 s^-1 -> 28.0 s^(-1); the catalog holds 28.0 s^(-1); no unit conversion applied (the paper already prints s^-1). Km: printed value 40 mM -> 40.0 mM; the catalog holds 40.0 mM; no unit conversion applied (the paper already prints mM). kcat/Km: kcat/Km: printed cell 0.70 s^-1 mM^-1 -> 0.7 s^(-1)*mM^(-1); the catalog holds 0.7 s^(-1)*mM^(-1); no unit conversion applied (the paper already prints s^-1 mM^-1). The printed cell is also the exact quotient of this row's own printed kcat and Km, 28 s^-1 / 40 mM.

row_label: Table III, substrate row `Xylitol (footnote a)`, Hamster column group


column_label: Table III (journal page 17888), substrate row `Xylitol (footnote a)`, Mouse column group: Km (mM) = 15; kcat (s^-1) = 17; kcat/Km (s^-1 mM^-1) = 1.1.

normalized_value_unit_evidence: kcat: printed value 17 s^-1 -> 17.0 s^(-1); the catalog holds 17.0 s^(-1); no unit conversion applied (the paper already prints s^-1). Km: printed value 15 mM -> 15.0 mM; the catalog holds 15.0 mM; no unit conversion applied (the paper already prints mM). kcat/Km: The catalog kcat_over_km 1.1333333333333333 s^(-1)*mM^(-1) is NOT a printed cell: it is the exact full-precision quotient of this row's own printed kcat and Km, 17 s^-1 / 15 mM. Table III does print a rounded kcat/Km cell for this row and species, `1.1` s^-1 mM^-1, which is stated here only for comparison; the catalog number itself remains derived, not quoted, so this pass types the ratio as derived_same_measurement and leaves the paper ratio null.

row_label: Table III, substrate row `Xylitol (footnote a)`, Mouse column group


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature K153M with inferred residue-numbering offset +8 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature Y149F to reconstruct the assayed variant sequence.


substrate_name: methylglyoxal

smiles: CC(=O)C=O

assay_conditions_summary: pH 7; 298.15 K; cofactor NADP+

column_label: Table II, decoded transcription, substrate row `Methylglyoxal`, Human column group: Km (mM) = 2.5; kcat (s^-1) = 0.57; kcat/Km (s^-1 mM^-1) = 0.23.

normalized_value_unit_evidence: kcat: printed cell 0.57 s^-1 -> 0.57 s^(-1); the catalog holds 0.57 s^(-1); no unit conversion applied (the decoded table header prints kcat in s^-1). Km: printed cell 2.5 mM -> 2.5 mM; the catalog holds 2.5 mM; no unit conversion applied (the decoded table header prints Km in mM). kcat/Km: The catalog kcat_over_km 0.22799999999999998 s^(-1)*mM^(-1) is NOT the printed cell: it is the exact full-precision quotient of this row's own printed kcat and Km, 0.57 s^-1 / 2.5 mM. The paper does print a rounded kcat/Km cell for this row, `0.23` s^-1 mM^-1; the exact quotient rounded to that cell's printed precision is 0.23, i.e. it equals the printed cell at printed precision, so the only difference is printed precision; the catalog number itself remains derived, not quoted.

table_label: Table II, decoded transcription

substrate: methylglyoxal


column_label: Table II, decoded transcription, substrate row `Methylglyoxal`, Mouse column group: Km (mM) = 8.1; kcat (s^-1) = 11; kcat/Km (s^-1 mM^-1) = 1.4.

normalized_value_unit_evidence: kcat: printed value 11 s^-1 -> 11.0 s^(-1); the catalog holds 11.0 s^(-1); no unit conversion applied (the decoded table already prints s^-1). Km: printed value 8.1 mM -> 8.1 mM; the catalog holds 8.1 mM; no unit conversion applied. kcat/Km: The catalog kcat_over_km 1.3580246913580247 s^(-1)*mM^(-1) is NOT a printed cell: it is the exact full-precision quotient of this row's own printed kcat and Km, 11 s^-1 / 8.1 mM, for the Mouse column group. The paper does print a rounded kcat/Km cell for this row and column, `1.4` s^-1 mM^-1; the exact quotient rounds (Decimal HALF_UP, 1 decimal place(s)) to 1.4, which EQUALS the printed cell, so the only difference is printed precision. The catalog number itself remains derived, not quoted, so this record types the ratio as derived_same_measurement and reports no paper ratio value.

table_label: TABLE II Substrate specificitiy for carbonyl compounds of rodent and human DCXRs


column_label: Table II, decoded transcription, substrate row `Methylglyoxal`, Rat column group: Km (mM) = 7.7; kcat (s^-1) = 5.6; kcat/Km (s^-1 mM^-1) = 0.72.

normalized_value_unit_evidence: kcat: printed value 5.6 s^-1 -> 5.6 s^(-1); the catalog holds 5.6 s^(-1); no unit conversion applied (the decoded table already prints s^-1). Km: printed value 7.7 mM -> 7.7 mM; the catalog holds 7.7 mM; no unit conversion applied. kcat/Km: The catalog kcat_over_km 0.7272727272727272 s^(-1)*mM^(-1) is NOT a printed cell: it is the exact full-precision quotient of this row's own printed kcat and Km, 5.6 s^-1 / 7.7 mM, for the Rat column group. The paper does print a rounded kcat/Km cell for this row and column, `0.72` s^-1 mM^-1; the exact quotient rounds (Decimal HALF_UP, 2 decimal place(s)) to 0.73, whereas the paper prints `0.72`, i.e. the paper's own printed ratio cell is rounded DOWN from the quotient of its own printed kcat and Km and disagrees with it in the last printed digit. The catalog number itself remains derived, not quoted, so this record types the ratio as derived_same_measurement and reports no paper ratio value.


enzyme_display_name: D-arabinitol 4-dehydrogenase

organism: Gluconobacter oxydans

substrate_name: D-arabinitol

enzyme: D-arabinitol 4-dehydrogenase

substrate: D-arabinitol


substrate_name: D-mannitol

substrate: D-mannitol


substrate_name: D-sorbitol

substrate: D-sorbitol


substrate_name: glycerol

smiles: OCC(O)CO

substrate: glycerol


substrate_name: ribitol

substrate: ribitol


enzyme_display_name: L-arabinitol 4-dehydrogenase

organism: Trichoderma reesei

enzyme: L-arabinitol 4-dehydrogenase


substrate_name: D-Fructose

substrate: D-Fructose


substrate_name: D-Psicose

substrate: D-Psicose


organism: Meyerozyma caribbica


organism: Rasamsonia emersonii


substrate_name: D-Sorbose

substrate: D-Sorbose


substrate_name: D-Talitol

substrate: D-Talitol


organism: Aspergillus niger

substrate_name: L-arabinitol

verification_status: corrected

assay_conditions_summary: pH 7; 295.15 K; buffer 50 mM potassium phosphate; cofactor NAD+

column_label: Table 1 (Kinetic parameters of LADs for L-arabinitol at saturated cofactor concentrations), anLAD NAD+ line; column 'Km (mM)' = 25±1; column 'kcat (min-1)' = 507±22; column 'kcat/Km (mM-1 min-1)' = 20.0±0.8; column 'Specific activity (U/mg protein)' = 11.7±0.3.

normalized_value_unit_evidence: Km: printed Table 1 cell 25±1 mM; the printed head is already mM, so no unit conversion applies and the canonical value is 25.0 mM. kcat: printed Table 1 cell 507±22 min^-1; the printed head is per minute, so 507 / 60 = 8.45 s^(-1). kcat/Km: printed Table 1 cell 20.0±0.8 mM^-1 min^-1; one per-minute rate is one sixtieth of the same per-second rate, so 20.0 / 60 = 0.3333333333333333 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 1, anLAD NAD+ line: Km cell = 25±1 mM; kcat cell = 507±22 min^-1; printed kcat/Km cell = 20.0±0.8 mM^-1 min^-1.

row_label: Table 1, anLAD NAD+ line for L-arabinitol at the saturating NAD+ concentration

table_label: Table 1: Kinetic parameters of LADs for L-arabinitol at saturated cofactor concentrations (Appl Microbiol Biotechnol 2010, 87:1407-1414), PDF page 4 of 8; printed column heads enzyme, cofactor, Specific activity (U/mg protein), Km (mM), kcat (min-1) and kcat/Km (mM-1 min-1)

substrate: L-arabinitol

status: corrected


organism: Penicillium chrysogenum

column_label: Table 1 (Kinetic parameters of LADs for L-arabinitol at saturated cofactor concentrations), pcLAD NAD+ line; column 'Km (mM)' = 37±2; column 'kcat (min-1)' = 1,085±71; column 'kcat/Km (mM-1 min-1)' = 29±1; column 'Specific activity (U/mg protein)' = 25.3±1.4.

normalized_value_unit_evidence: Km: printed Table 1 cell 37±2 mM; the printed head is already mM, so no unit conversion applies and the canonical value is 37.0 mM. kcat: printed Table 1 cell 1,085±71 min^-1; the printed head is per minute, so 1,085 / 60 = 18.083333333333332 s^(-1). kcat/Km: printed Table 1 cell 29±1 mM^-1 min^-1; one per-minute rate is one sixtieth of the same per-second rate, so 29 / 60 = 0.48333333333333334 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 1, pcLAD NAD+ line: Km cell = 37±2 mM; kcat cell = 1,085±71 min^-1; printed kcat/Km cell = 29±1 mM^-1 min^-1.

row_label: Table 1, pcLAD NAD+ line for L-arabinitol at the saturating NAD+ concentration


substrate_name: galactitol

substrate: galactitol


substrate_name: L-Iditol

substrate: L-Iditol


substrate_name: L-Mannitol

substrate: L-Mannitol


substrate_name: L-Sorbose

substrate: L-Sorbose


substrate_name: L-Tagatose

substrate: L-Tagatose


substrate_name: NAD+

column_label: Table 2 (Kinetic parameters of LADs for NAD+ and NADP+ at saturated L-arabinitol concentration), anLAD NAD+ line; column 'Km (mM)' = 0.20±0.01; column 'kcat (min-1)' = 494±11; column 'kcat/Km (mM-1 min-1)' = 2,526±83.

normalized_value_unit_evidence: Km: printed Table 2 cell 0.20±0.01 mM; the printed head is already mM, so no unit conversion applies and the canonical value is 0.2 mM. kcat: printed Table 2 cell 494±11 min^-1; the printed head is per minute, so 494 / 60 = 8.233333333333333 s^(-1). kcat/Km: printed Table 2 cell 2,526±83 mM^-1 min^-1; one per-minute rate is one sixtieth of the same per-second rate, so 2,526 / 60 = 42.1 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, anLAD NAD+ line: Km cell = 0.20±0.01 mM; kcat cell = 494±11 min^-1; printed kcat/Km cell = 2,526±83 mM^-1 min^-1.

row_label: Table 2, anLAD NAD+ line for the varied cofactor NAD+ at the saturating L-arabinitol concentration

table_label: Table 2: Kinetic parameters of LADs for NAD+ and NADP+ at saturated L-arabinitol concentration (Appl Microbiol Biotechnol 2010, 87:1407-1414), PDF page 5 of 8; printed column heads enzyme, cofactor, Km (mM), kcat (min-1) and kcat/Km (mM-1 min-1)

substrate: NAD+


assay_conditions_summary: pH 7; 303.15 K; cofactor NAD+


organism: Neurospora crassa

sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature D211S with inferred residue-numbering offset +2 to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 8; 298.15 K; cofactor NAD+


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature D211S/I212R to reconstruct the assayed variant sequence.


column_label: Table 2 (Kinetic parameters of LADs for NAD+ and NADP+ at saturated L-arabinitol concentration), pcLAD NAD+ line; column 'Km (mM)' = 0.3±0.1; column 'kcat (min-1)' = 1,039±165; column 'kcat/Km (mM-1 min-1)' = 3,460±505.

normalized_value_unit_evidence: Km: printed Table 2 cell 0.3±0.1 mM; the printed head is already mM, so no unit conversion applies and the canonical value is 0.3 mM. kcat: printed Table 2 cell 1,039±165 min^-1; the printed head is per minute, so 1,039 / 60 = 17.316666666666666 s^(-1). kcat/Km: printed Table 2 cell 3,460±505 mM^-1 min^-1; one per-minute rate is one sixtieth of the same per-second rate, so 3,460 / 60 = 57.666666666666664 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, pcLAD NAD+ line: Km cell = 0.3±0.1 mM; kcat cell = 1,039±165 min^-1; printed kcat/Km cell = 3,460±505 mM^-1 min^-1.

row_label: Table 2, pcLAD NAD+ line for the varied cofactor NAD+ at the saturating L-arabinitol concentration


assay_conditions_summary: pH 9; 303.15 K; cofactor NAD+


column_label: Table 2 (Kinetic parameters of LADs for NAD+ and NADP+ at saturated L-arabinitol concentration), tlLAD NAD+ line; column 'Km (mM)' = 0.2±0.1; column 'kcat (min-1)' = 436±96; column 'kcat/Km (mM-1 min-1)' = 2,689±646.

normalized_value_unit_evidence: Km: printed Table 2 cell 0.2±0.1 mM; the printed head is already mM, so no unit conversion applies and the canonical value is 0.2 mM. kcat: printed Table 2 cell 436±96 min^-1; the printed head is per minute, so 436 / 60 = 7.266666666666667 s^(-1). kcat/Km: printed Table 2 cell 2,689±646 mM^-1 min^-1; one per-minute rate is one sixtieth of the same per-second rate, so 2,689 / 60 = 44.81666666666666 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, tlLAD NAD+ line: Km cell = 0.2±0.1 mM; kcat cell = 436±96 min^-1; printed kcat/Km cell = 2,689±646 mM^-1 min^-1.

row_label: Table 2, tlLAD NAD+ line for the varied cofactor NAD+ at the saturating L-arabinitol concentration


substrate_name: NADP+

assay_conditions_summary: pH 7; 303.15 K; cofactor NADP+

substrate: NADP+


assay_conditions_summary: pH 8; 298.15 K; cofactor NADP+


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature D211S/I212R/D213N to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature D211S/I212R/S348T to reconstruct the assayed variant sequence.


substrate_name: xylitol

substrate: xylitol


substrate_name: L-Arabitol

substrate: L-Arabitol


enzyme_display_name: L-iditol 2-dehydrogenase

organism: Ovis aries

enzyme: L-iditol 2-dehydrogenase


smiles: CCCCC(O)C(O)CO

column_label: Table 2 (journal page 482), substrate row `1,2,3-heptanetriol (70% DL-threo, 30% DL-erythro)`, pH 9.9 column group: kcat (s-1) = 1.7; Km (mM) = 0.38; kcat/Km (s-1 M-1) = 4474.

normalized_value_unit_evidence: kcat: printed value 1.7 s-1 -> 1.7 s^(-1); the catalog holds 1.7 s^(-1); no unit conversion applied (the paper already prints s-1). Km: printed value 0.38 mM -> 0.38 mM; the catalog holds 0.38 mM; no unit conversion applied. kcat/Km: The catalog kcat_over_km 4.473684210526316 s^(-1)*mM^(-1) is NOT a printed cell: it is the exact full-precision quotient of this row's own printed kcat and Km, 1.7 s-1 / 0.38 mM. The paper does print a rounded kcat/Km cell for this row, `4474` s-1 M-1 = 4.474 s^(-1)*mM^(-1); the exact quotient rounds to that printed value, so the only difference is printed precision; the catalog number itself remains derived, not quoted.

row_label: Table 2, substrate row `1,2,3-heptanetriol (70% DL-threo, 30% DL-erythro)`, pH 9.9 column group

table_label: Table 2 Kinetic constants for deoxy polyol substrates of sorbitol dehydrogenase


smiles: CCCC(O)C(O)CO


smiles: CCCCCC(O)C(O)CO


smiles: OCC(O)CCCCC(O)CO

column_label: Table 2 (journal page 482), substrate row `1,2,7,8-octanetetrol (mixture of D-, L- and meso forms)`, pH 9.9 column group: kcat (s-1) = 0.50; Km (mM) = 40; kcat/Km (s-1 M-1) = 13.

normalized_value_unit_evidence: kcat: printed value 0.50 s-1 -> 0.5 s^(-1); the catalog holds 0.5 s^(-1); no unit conversion applied (the paper already prints s-1). Km: printed value 40 mM -> 40.0 mM; the catalog holds 40.0 mM; no unit conversion applied. kcat/Km: The catalog kcat_over_km 0.0125 s^(-1)*mM^(-1) is NOT a printed cell: it is the exact full-precision quotient of this row's own printed kcat and Km, 0.50 s-1 / 40 mM. The paper does print a rounded kcat/Km cell for this row, `13` s-1 M-1 = 0.013 s^(-1)*mM^(-1); the exact quotient rounds to that printed value, so the only difference is printed precision; the catalog number itself remains derived, not quoted.

row_label: Table 2, substrate row `1,2,7,8-octanetetrol (mixture of D-, L- and meso forms)`, pH 9.9 column group


smiles: CC(O)C(C)O


smiles: C=CC(O)CO


smiles: COCC(O)C(O)COC

column_label: Table 2 (journal page 482), substrate row `(2S, 3S)-1,4-dimethoxy-2,3-butanediol`, pH 9.9 column group: kcat (s-1) = 0.50; Km (mM) = 85; kcat/Km (s-1 M-1) = 5.9.

normalized_value_unit_evidence: kcat: printed value 0.50 s-1 -> 0.5 s^(-1); the catalog holds 0.5 s^(-1); no unit conversion applied (the paper already prints s-1). Km: printed value 85 mM -> 85.0 mM; the catalog holds 85.0 mM; no unit conversion applied. kcat/Km: The catalog kcat_over_km 0.0058823529411764705 s^(-1)*mM^(-1) is NOT a printed cell: it is the exact full-precision quotient of this row's own printed kcat and Km, 0.50 s-1 / 85 mM. The paper does print a rounded kcat/Km cell for this row, `5.9` s-1 M-1 = 0.005900000000000001 s^(-1)*mM^(-1); the exact quotient rounds to that printed value, so the only difference is printed precision; the catalog number itself remains derived, not quoted.

row_label: Table 2, substrate row `(2S, 3S)-1,4-dimethoxy-2,3-butanediol`, pH 9.9 column group


column_label: Table 2 (journal page 482), substrate row `6-deoxy-6-nitro-D-iditol`, pH 7.4 column group: kcat (s-1) = 0.28; Km (mM) = 184; kcat/Km (s-1 M-1) = 1.5.

normalized_value_unit_evidence: kcat: printed value 0.28 s-1 -> 0.28 s^(-1); the catalog holds 0.28 s^(-1); no unit conversion applied (the paper already prints s-1). Km: printed value 184 mM -> 184.0 mM; the catalog holds 184.0 mM; no unit conversion applied. kcat/Km: The catalog kcat_over_km 0.0015217391304347828 s^(-1)*mM^(-1) is NOT a printed cell: it is the exact full-precision quotient of this row's own printed kcat and Km, 0.28 s-1 / 184 mM. The paper does print a rounded kcat/Km cell for this row, `1.5` s-1 M-1 = 0.0015 s^(-1)*mM^(-1); the exact quotient rounds to that printed value, so the only difference is printed precision; the catalog number itself remains derived, not quoted.

row_label: Table 2, substrate row `6-deoxy-6-nitro-D-iditol`, pH 7.4 column group


smiles: CC(O)C(O)C(O)C(O)CO

column_label: Table 2 (journal page 482), substrate row `6-deoxy-L-mannitol (L-rhamnitol)`, pH 9.9 column group: kcat (s-1) = 0.040; Km (mM) = 3.8; kcat/Km (s-1 M-1) = 11.

normalized_value_unit_evidence: kcat: printed value 0.040 s-1 -> 0.04 s^(-1); the catalog holds 0.04 s^(-1); no unit conversion applied (the paper already prints s-1). Km: printed value 3.8 mM -> 3.8 mM; the catalog holds 3.8 mM; no unit conversion applied. kcat/Km: The catalog kcat_over_km 0.010526315789473686 s^(-1)*mM^(-1) is NOT a printed cell: it is the exact full-precision quotient of this row's own printed kcat and Km, 0.040 s-1 / 3.8 mM. The paper does print a rounded kcat/Km cell for this row, `11` s-1 M-1 = 0.011 s^(-1)*mM^(-1); the exact quotient rounds to that printed value, so the only difference is printed precision; the catalog number itself remains derived, not quoted.

row_label: Table 2, substrate row `6-deoxy-L-mannitol (L-rhamnitol)`, pH 9.9 column group


substrate_name: D-Allitol

substrate: D-Allitol


substrate_name: D-Altritol

substrate: D-Altritol


substrate_name: D-Arabitol

substrate: D-Arabitol


substrate_name: D-Fructose

column_label: Main text (Results, journal page 481), D-fructose oxidation to 5-keto-D-fructose at pH 9.9 (Results, journal page 481): kcat = 0.25 s-1; Km = 825 mM. The sentence prints no kcat/Km value.

normalized_value_unit_evidence: kcat: printed 0.25 s-1 -> 0.25 s^(-1); the catalog holds 0.25 s^(-1); no unit conversion applied (the paper prints s-1 directly). Km: printed 825 mM -> 825.0 mM; the catalog holds 825.0 mM; no unit conversion applied (the paper prints mM directly). kcat/Km: The catalog kcat_over_km 0.00030303030303030303 s^(-1)*mM^(-1) is not a printed value: this paper prints no kcat/Km for D-fructose, so the exported ratio is the exact full-precision quotient of this measurement's own printed kcat and Km, 0.25 s-1 / 825 mM, typed as derived_same_measurement.

table_label: Main text (Results, journal page 481)

substrate: D-Fructose


substrate_name: D-glycero-L-allo-Heptitol

substrate: D-glycero-L-allo-Heptitol


substrate_name: D-glycero-L-galacto-Heptitol

substrate: D-glycero-L-galacto-Heptitol


substrate_name: D-Iditol

substrate: D-Iditol


organism: Bos taurus

substrate_name: D-Mannitol

column_label: TABLE II  Kinetic Constants and Substrate Specificity of Bovine Lens SDH; substrate row `D-Mannitol`; column `KM (mM)` = 381 +/- 21; column `kcat (s^-1)` = 24 +/- 2; column `kcat/KM (s^-1 mM^-1)` = 0.063.

normalized_value_unit_evidence: kcat: printed cell 24 +/- 2 s^-1 is already in canonical units, 24.0 s^(-1). KM: printed cell 381 +/- 21 mM is already in canonical units, 381.0 mM (Table II prints KM in mM). kcat/KM: printed cell 0.063 s^-1 mM^-1; the exact quotient of the paper's own two cells is 24 / 381 = 0.06299212598425197 s^-1 mM^-1.

raw_value_unit_evidence: TABLE II (printed page 386), substrate row `D-Mannitol`: KM = 381 +/- 21 mM; kcat = 24 +/- 2 s^-1; kcat/KM = 0.063 s^-1 mM^-1.

row_label: TABLE II, substrate row `D-Mannitol`

substrate: D-Mannitol


substrate_name: D-Ribulose

column_label: TABLE II  Kinetic Constants and Substrate Specificity of Bovine Lens SDH; substrate row `D-Ribulose`; column `KM (mM)` = 260 +/- 7; column `kcat (s^-1)` = 198 +/- 5; column `kcat/KM (s^-1 mM^-1)` = 0.76.

normalized_value_unit_evidence: kcat: printed cell 198 +/- 5 s^-1 is already in canonical units, 198.0 s^(-1). KM: printed cell 260 +/- 7 mM is already in canonical units, 260.0 mM (Table II prints KM in mM). kcat/KM: printed cell 0.76 s^-1 mM^-1; the exact quotient of the paper's own two cells is 198 / 260 = 0.7615384615384615 s^-1 mM^-1.

raw_value_unit_evidence: TABLE II (printed page 386), substrate row `D-Ribulose`: KM = 260 +/- 7 mM; kcat = 198 +/- 5 s^-1; kcat/KM = 0.76 s^-1 mM^-1.

row_label: TABLE II, substrate row `D-Ribulose`

substrate: D-Ribulose


substrate_name: D-Sorbitol

column_label: TABLE II  Kinetic Constants and Substrate Specificity of Bovine Lens SDH; substrate row `D-Sorbitol`; column `KM (mM)` = 18 +/- 0.4; column `kcat (s^-1)` = 29 +/- 0.1; column `kcat/KM (s^-1 mM^-1)` = 1.59.

normalized_value_unit_evidence: kcat: printed cell 29 +/- 0.1 s^-1 is already in canonical units, 29.0 s^(-1). KM: printed cell 18 +/- 0.4 mM is already in canonical units, 18.0 mM (Table II prints KM in mM). kcat/KM: printed cell 1.59 s^-1 mM^-1; the exact quotient of the paper's own two cells is 29 / 18 = 1.6111111111111112 s^-1 mM^-1.

raw_value_unit_evidence: TABLE II (printed page 386), substrate row `D-Sorbitol`: KM = 18 +/- 0.4 mM; kcat = 29 +/- 0.1 s^-1; kcat/KM = 1.59 s^-1 mM^-1.

row_label: TABLE II, substrate row `D-Sorbitol`

substrate: D-Sorbitol


substrate_name: Glycol

smiles: OCCO

substrate: Glycol


substrate_name: L-Altritol

smiles: OCC(O)C(O)C(O)C(O)CO

substrate: L-Altritol


substrate_name: L-Iditol

column_label: TABLE II  Kinetic Constants and Substrate Specificity of Bovine Lens SDH; substrate row `L-Iditol`; column `KM (mM)` = 20 +/- 1; column `kcat (s^-1)` = 21 +/- 0.1; column `kcat/KM (s^-1 mM^-1)` = 1.05.

normalized_value_unit_evidence: kcat: printed cell 21 +/- 0.1 s^-1 is already in canonical units, 21.0 s^(-1). KM: printed cell 20 +/- 1 mM is already in canonical units, 20.0 mM (Table II prints KM in mM). kcat/KM: printed cell 1.05 s^-1 mM^-1; the exact quotient of the paper's own two cells is 21 / 20 = 1.05 s^-1 mM^-1.

raw_value_unit_evidence: TABLE II (printed page 386), substrate row `L-Iditol`: KM = 20 +/- 1 mM; kcat = 21 +/- 0.1 s^-1; kcat/KM = 1.05 s^-1 mM^-1.

row_label: TABLE II, substrate row `L-Iditol`

substrate: L-Iditol


substrate_name: L-Mannitol

substrate: L-Mannitol


substrate_name: L-Sorbitol

substrate: L-Sorbitol


substrate_name: L-Sorbose

column_label: TABLE II  Kinetic Constants and Substrate Specificity of Bovine Lens SDH; substrate row `L-Sorbose`; column `KM (mM)` = 670 +/- 30; column `kcat (s^-1)` = 153 +/- 5; column `kcat/KM (s^-1 mM^-1)` = 0.23.

normalized_value_unit_evidence: kcat: printed cell 153 +/- 5 s^-1 is already in canonical units, 153.0 s^(-1). KM: printed cell 670 +/- 30 mM is already in canonical units, 670.0 mM (Table II prints KM in mM). kcat/KM: printed cell 0.23 s^-1 mM^-1; the exact quotient of the paper's own two cells is 153 / 670 = 0.22835820895522388 s^-1 mM^-1.

raw_value_unit_evidence: TABLE II (printed page 386), substrate row `L-Sorbose`: KM = 670 +/- 30 mM; kcat = 153 +/- 5 s^-1; kcat/KM = 0.23 s^-1 mM^-1.

row_label: TABLE II, substrate row `L-Sorbose`

substrate: L-Sorbose


column_label: Main text (Results, journal page 481), L-sorbose oxidation to 5-keto-D-fructose at pH 9.9 (Results, journal page 481): kcat = 0.83 s-1; Km = 240 mM. The sentence prints no kcat/Km value.

normalized_value_unit_evidence: kcat: printed 0.83 s-1 -> 0.83 s^(-1); the catalog holds 0.83 s^(-1); no unit conversion applied (the paper prints s-1 directly). Km: printed 240 mM -> 240.0 mM; the catalog holds 240.0 mM; no unit conversion applied (the paper prints mM directly). kcat/Km: The catalog kcat_over_km 0.0034583333333333332 s^(-1)*mM^(-1) is not a printed value: this paper prints no kcat/Km for L-sorbose, so the exported ratio is the exact full-precision quotient of this measurement's own printed kcat and Km, 0.83 s-1 / 240 mM, typed as derived_same_measurement.


substrate_name: L-Threitol

column_label: TABLE II  Kinetic Constants and Substrate Specificity of Bovine Lens SDH; substrate row `L-Threitol`; column `KM (mM)` = 268 +/- 14; column `kcat (s^-1)` = 12 +/- 0.7; column `kcat/KM (s^-1 mM^-1)` = 0.045.

normalized_value_unit_evidence: kcat: printed cell 12 +/- 0.7 s^-1 is already in canonical units, 12.0 s^(-1). KM: printed cell 268 +/- 14 mM is already in canonical units, 268.0 mM (Table II prints KM in mM). kcat/KM: printed cell 0.045 s^-1 mM^-1; the exact quotient of the paper's own two cells is 12 / 268 = 0.04477611940298507 s^-1 mM^-1.

raw_value_unit_evidence: TABLE II (printed page 386), substrate row `L-Threitol`: KM = 268 +/- 14 mM; kcat = 12 +/- 0.7 s^-1; kcat/KM = 0.045 s^-1 mM^-1.

row_label: TABLE II, substrate row `L-Threitol`

substrate: L-Threitol


substrate_name: NAD+

assay_conditions_summary: pH 8; 310.15 K; cofactor NAD+

column_label: TABLE II  Kinetic Constants and Substrate Specificity of Bovine Lens SDH; substrate row `NAD+ (b)`; column `KM (mM)` = 0.089 +/- 0.002; column `kcat (s^-1)` = 26 +/- 0.3; column `kcat/KM (s^-1 mM^-1)` = 292.

normalized_value_unit_evidence: kcat: printed cell 26 +/- 0.3 s^-1 is already in canonical units, 26.0 s^(-1). KM: printed cell 0.089 +/- 0.002 mM is already in canonical units, 0.089 mM (Table II prints KM in mM). kcat/KM: printed cell 292 s^-1 mM^-1; the exact quotient of the paper's own two cells is 26 / 0.089 = 292.13483146067415 s^-1 mM^-1.

raw_value_unit_evidence: TABLE II (printed page 386), substrate row `NAD+ (b)`: KM = 0.089 +/- 0.002 mM; kcat = 26 +/- 0.3 s^-1; kcat/KM = 292 s^-1 mM^-1.

row_label: TABLE II, substrate row `NAD+ (b)`

substrate: NAD+


assay_conditions_summary: pH 8; 303.15 K; cofactor NAD+


substrate_name: Phenylglyoxal

assay_conditions_summary: pH 7.4; 296.65 K; cofactor NADH

column_label: Main text (Results, journal page 483), 2-phenyl-2-oxoacetaldehyde (phenylglyoxal) reduction with 100 uM NADH at pH 7.4 (Results, journal page 483): kcat = 13 s-1; Km = 88 mM. The sentence prints no kcat/Km value.

normalized_value_unit_evidence: kcat: printed 13 s-1 -> 13.0 s^(-1); the catalog holds 13.0 s^(-1); no unit conversion applied (the paper prints s-1 directly). Km: printed 88 mM -> 88.0 mM; the catalog holds 88.0 mM; no unit conversion applied (the paper prints mM directly). kcat/Km: The catalog kcat_over_km 0.14772727272727273 s^(-1)*mM^(-1) is not a printed value: this paper prints no kcat/Km for phenylglyoxal, so the exported ratio is the exact full-precision quotient of this measurement's own printed kcat and Km, 13 s-1 / 88 mM, typed as derived_same_measurement.

table_label: Main text (Results, journal page 483)

substrate: Phenylglyoxal


smiles: OCCC(O)CO


smiles: CCC(O)CO


smiles: CCCC(O)CO


column_label: Table 3 (journal page 482), substrate row `(S)-1-O-benzylglycerol`, pH 9.9 column group: kcat (s-1) = 0.63; Km (mM) = 2.7; kcat/Km (s-1 M-1) = 233.

normalized_value_unit_evidence: kcat: printed value 0.63 s-1 -> 0.63 s^(-1); the catalog holds 0.63 s^(-1); no unit conversion applied (the paper already prints s-1). Km: printed value 2.7 mM -> 2.7 mM; the catalog holds 2.7 mM; no unit conversion applied. kcat/Km: The catalog kcat_over_km 0.2333333333333333 s^(-1)*mM^(-1) is NOT a printed cell: it is the exact full-precision quotient of this row's own printed kcat and Km, 0.63 s-1 / 2.7 mM. The paper does print a rounded kcat/Km cell for this row, `233` s-1 M-1 = 0.233 s^(-1)*mM^(-1); the exact quotient rounds to that printed value, so the only difference is printed precision; the catalog number itself remains derived, not quoted.

row_label: Table 3, substrate row `(S)-1-O-benzylglycerol`, pH 9.9 column group

table_label: Table 3 Kinetic constants for aromatic substrates of sorbitol dehydrogenase


column_label: Table 3 (journal page 482), substrate row `(S)-1-phenylethanol`, pH 9.9 column group: kcat (s-1) = 0.10; Km (mM) = 7.0; kcat/Km (s-1 M-1) = 14.

normalized_value_unit_evidence: kcat: printed value 0.10 s-1 -> 0.1 s^(-1); the catalog holds 0.1 s^(-1); no unit conversion applied (the paper already prints s-1). Km: printed value 7.0 mM -> 7.0 mM; the catalog holds 7.0 mM; no unit conversion applied. kcat/Km: The catalog kcat_over_km 0.014285714285714287 s^(-1)*mM^(-1) is NOT a printed cell: it is the exact full-precision quotient of this row's own printed kcat and Km, 0.10 s-1 / 7.0 mM. The paper does print a rounded kcat/Km cell for this row, `14` s-1 M-1 = 0.014 s^(-1)*mM^(-1); the exact quotient rounds to that printed value, so the only difference is printed precision; the catalog number itself remains derived, not quoted.

row_label: Table 3, substrate row `(S)-1-phenylethanol`, pH 9.9 column group


column_label: Table 2 (journal page 482), substrate row `(S)-2,4-butanediol`, pH 9.9 column group: kcat (s-1) = 0.063; Km (mM) = 70; kcat/Km (s-1 M-1) = 0.90.

normalized_value_unit_evidence: kcat: printed value 0.063 s-1 -> 0.063 s^(-1); the catalog holds 0.063 s^(-1); no unit conversion applied (the paper already prints s-1). Km: printed value 70 mM -> 70.0 mM; the catalog holds 70.0 mM; no unit conversion applied. kcat/Km: The paper PRINTS the kcat/Km cell `0.90` s-1 M-1 for this row; 0.90 / 1000 = 0.0009 s^(-1)*mM^(-1), which EQUALS the catalog kcat_over_km 0.0009 exactly (it also equals the exact quotient 0.063 / 70 of the row's own printed kcat and Km).

row_label: Table 2, substrate row `(S)-2,4-butanediol`, pH 9.9 column group


substrate_name: ribitol

column_label: TABLE II  Kinetic Constants and Substrate Specificity of Bovine Lens SDH; substrate row `Ribitol`; column `KM (mM)` = 67 +/- 3; column `kcat (s^-1)` = 22 +/- 0.6; column `kcat/KM (s^-1 mM^-1)` = 0.33.

normalized_value_unit_evidence: kcat: printed cell 22 +/- 0.6 s^-1 is already in canonical units, 22.0 s^(-1). KM: printed cell 67 +/- 3 mM is already in canonical units, 67.0 mM (Table II prints KM in mM). kcat/KM: printed cell 0.33 s^-1 mM^-1; the exact quotient of the paper's own two cells is 22 / 67 = 0.3283582089552239 s^-1 mM^-1.

raw_value_unit_evidence: TABLE II (printed page 386), substrate row `Ribitol`: KM = 67 +/- 3 mM; kcat = 22 +/- 0.6 s^-1; kcat/KM = 0.33 s^-1 mM^-1.

row_label: TABLE II, substrate row `Ribitol`

substrate: ribitol


substrate_name: xylitol

column_label: TABLE II  Kinetic Constants and Substrate Specificity of Bovine Lens SDH; substrate row `Xylitol`; column `KM (mM)` = 3.2 +/- 0.2; column `kcat (s^-1)` = 26 +/- 0.4; column `kcat/KM (s^-1 mM^-1)` = 8.1.

normalized_value_unit_evidence: kcat: printed cell 26 +/- 0.4 s^-1 is already in canonical units, 26.0 s^(-1). KM: printed cell 3.2 +/- 0.2 mM is already in canonical units, 3.2 mM (Table II prints KM in mM). kcat/KM: printed cell 8.1 s^-1 mM^-1; the exact quotient of the paper's own two cells is 26 / 3.2 = 8.125 s^-1 mM^-1.

raw_value_unit_evidence: TABLE II (printed page 386), substrate row `Xylitol`: KM = 3.2 +/- 0.2 mM; kcat = 26 +/- 0.4 s^-1; kcat/KM = 8.1 s^-1 mM^-1.

row_label: TABLE II, substrate row `Xylitol`

substrate: xylitol


enzyme_display_name: galactitol 2-dehydrogenase

organism: Rhizobium leguminosarum

substrate_name: galactitol

enzyme: galactitol 2-dehydrogenase

substrate: galactitol


enzyme_display_name: mannitol-1-phosphate 5-dehydrogenase

organism: Aspergillus fumigatus

substrate_name: D-Fructose 6-phosphate

enzyme: mannitol-1-phosphate 5-dehydrogenase

substrate: D-Fructose 6-phosphate


substrate_name: D-Mannitol 1-phosphate

substrate: D-Mannitol 1-phosphate


assay_conditions_summary: pH 7.1; 298.15 K; cofactor NAD+


substrate_name: NADH

assay_conditions_summary: pH 7.1; 298.15 K; cofactor NADH

substrate: NADH


substrate_name: D-erythrose

source_db: brenda

sequence_source: multiple

smiles: C(C(C(C=O)O)O)O

assay_conditions_summary: pH 7; cofactor NADPH

proof_lines: kcat is 24 sec(-1) with diacetyl as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:11882650). kcat is 37 sec(-1) with L-xylulose as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:11882650). kcat is 13 sec(-1) with 1,4-dibromo-2,

source_databases_merged: brenda uniprot

substrate: D-erythrose

source: brenda


substrate_name: acetoin

smiles: CC(C(=O)C)O

proof_lines: kcat is 41 sec(-1) with diacetyl as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:11882650). kcat is 37 sec(-1) with L-xylulose as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:11882650). kcat is 42 sec(-1) with 1,4-dibromo-2,

substrate: acetoin


substrate_name: dihydroxyacetone

smiles: C(C(=O)CO)O

substrate: dihydroxyacetone


substrate_name: D-xylulose

smiles: C(C(C(C(=O)CO)O)O)O

substrate: D-xylulose


substrate_name: D-ribulose

substrate: D-ribulose


substrate_name: NADP+

assay_conditions_summary: pH 7; cofactor NADP+

substrate: NADP+


substrate_name: D-threitol

smiles: C(C(C(CO)O)O)O

substrate: D-threitol


substrate_name: L-threose

substrate: L-threose


smiles: C(C(C(C(CO)O)O)O)O


substrate_name: DL-glyceraldehyde

smiles: C(C(C=O)O)O

substrate: DL-glyceraldehyde


substrate_name: L-erythrulose

smiles: C(C(C(=O)CO)O)O

substrate: L-erythrulose


substrate_name: diacetyl

smiles: CC(=O)C(=O)C

substrate: diacetyl


substrate_name: NADPH

substrate: NADPH


substrate_name: NAD+

substrate: NAD+


substrate_name: diacetyl (at pH 7.0 and 25 degrees Celsius)

source_db: uniprot

kcat_display: Not reported

kcat_over_km_display: Not reported

proof_lines: kcat is 1.6 sec(-1) with diacetyl as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:11882650, PubMed:15103634). kcat is 0.65 sec(-1) with L-xylulose as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:11882650). kcat is 0.48 sec(-

source: uniprot


substrate_name: L-xylulose (at pH 7.0 and 25 degrees Celsius)


substrate_name: 1,4-dibromo-2,3-butanedione (at pH 7.0 and 25 degrees Celsius)

smiles: C(C(=O)C(=O)CBr)Br


substrate_name: 2,3-pentanedione (at pH 7.0 and 25 degrees Celsius)

smiles: CCC(=O)C(=O)C


substrate_name: 2,3-hexanedione (at pH 7.0 and 25 degrees Celsius)

smiles: CCCC(=O)C(=O)C


substrate_name: 3,4-hexanedione (at pH 7.0 and 25 degrees Celsius)

smiles: CCC(=O)C(=O)CC


substrate_name: 2,3-heptanedione (at pH 7.0 and 25 degrees Celsius)

smiles: CCCCC(=O)C(=O)C


substrate_name: isatin (at pH 7.0 and 25 degrees Celsius)


substrate_name: 1-phenyl-1,2-propanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: propioin (at pH 7.0 and 25 degrees Celsius)

smiles: CCC(C(=O)CC)O


substrate_name: acetoin (at pH 7.0 and 25 degrees Celsius)


substrate_name: methylglyoxal (at pH 7.0 and 25 degrees Celsius)

smiles: CC(=O)C=O


substrate_name: NADPH (at pH 7.0 and 25 degrees Celsius)


substrate_name: NADH (at pH 7.0 and 25 degrees Celsius)

substrate: NADH (at pH 7.0 and 25 degrees Celsius)


substrate_name: dihydroxyacetone (at pH 7.0 and 25 degrees Celsius)


substrate_name: DL-glyceraldehyde (at pH 7.0 and 25 degrees Celsius)


substrate_name: L-erythrulose (at pH 7.0 and 25 degrees Celsius)


substrate_name: D-erythrose (at pH 7.0 and 25 degrees Celsius)


substrate_name: D-threitol (at pH 7.0 and 25 degrees Celsius)


substrate_name: xylitol (at pH 7.0 and 25 degrees Celsius)


substrate_name: NADP(+) (at pH 7.0 and 25 degrees Celsius)


substrate_name: NAD(+) (at pH 7.0 and 25 degrees Celsius)


substrate_name: 1,4-dibromo-2,3-butanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: 2,3-pentanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: 2,3-hexanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: 3,4-hexanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: 2,3-heptanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: isatin (at pH 7.0 and 25 degrees Celsius)


substrate_name: 1-phenyl-1,2-propanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: propioin (at pH 7.0 and 25 degrees Celsius)


substrate_name: methylglyoxal (at pH 7.0 and 25 degrees Celsius)


substrate: NADP(+) (at pH 7.0 and 25 degrees Celsius)


substrate_name: diacetyl (at pH 7.0 and 25 degrees Celsius)

proof_lines: kcat is 25 sec(-1) with diacetyl as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:11882650). kcat is 39 sec(-1) with L-xylulose as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:11882650). kcat is 22 sec(-1) with 1,4-dibromo-2,


substrate_name: L-xylulose (at pH 7.0 and 25 degrees Celsius)


substrate: 1,4-dibromo-2,3-butanedione (at pH 7.0 and 25 degrees Celsius)


substrate: 2,3-pentanedione (at pH 7.0 and 25 degrees Celsius)


substrate: 2,3-hexanedione (at pH 7.0 and 25 degrees Celsius)


substrate: 3,4-hexanedione (at pH 7.0 and 25 degrees Celsius)


substrate: 2,3-heptanedione (at pH 7.0 and 25 degrees Celsius)


substrate: isatin (at pH 7.0 and 25 degrees Celsius)


substrate: 1-phenyl-1,2-propanedione (at pH 7.0 and 25 degrees Celsius)


substrate: propioin (at pH 7.0 and 25 degrees Celsius)


substrate_name: acetoin (at pH 7.0 and 25 degrees Celsius)


substrate: methylglyoxal (at pH 7.0 and 25 degrees Celsius)


substrate_name: NADPH (at pH 7.0 and 25 degrees Celsius)


substrate_name: NADH (at pH 7.0 and 25 degrees Celsius)


substrate_name: dihydroxyacetone (at pH 7.0 and 25 degrees Celsius)


substrate_name: DL-glyceraldehyde (at pH 7.0 and 25 degrees Celsius)


substrate_name: L-erythrulose (at pH 7.0 and 25 degrees Celsius)


substrate_name: D-erythrose (at pH 7.0 and 25 degrees Celsius)


substrate_name: D-threitol (at pH 7.0 and 25 degrees Celsius)


substrate_name: xylitol (at pH 7.0 and 25 degrees Celsius)


substrate_name: NADP(+) (at pH 7.0 and 25 degrees Celsius)


substrate_name: NAD(+) (at pH 7.0 and 25 degrees Celsius)


proof_lines: kcat is 30 sec(-1) with diacetyl as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:11882650). kcat is 36 sec(-1) with L-xylulose as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:11882650). kcat is 15 sec(-1) with 1,4-dibromo-2,

substrate: diacetyl (at pH 7.0 and 25 degrees Celsius)


substrate: L-xylulose (at pH 7.0 and 25 degrees Celsius)


substrate_name: 1,4-dibromo-2,3-butanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: 2,3-pentanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: 2,3-hexanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: 3,4-hexanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: 2,3-heptanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: isatin (at pH 7.0 and 25 degrees Celsius)


substrate_name: 1-phenyl-1,2-propanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: propioin (at pH 7.0 and 25 degrees Celsius)


substrate_name: acetoin (at pH 7.0 and 25 degrees Celsius)


substrate_name: methylglyoxal (at pH 7.0 and 25 degrees Celsius)


substrate_name: NADPH (at pH 7.0 and 25 degrees Celsius)


substrate_name: NADH (at pH 7.0 and 25 degrees Celsius)


substrate_name: dihydroxyacetone (at pH 7.0 and 25 degrees Celsius)


substrate_name: DL-glyceraldehyde (at pH 7.0 and 25 degrees Celsius)


substrate_name: L-erythrulose (at pH 7.0 and 25 degrees Celsius)


substrate_name: D-erythrose (at pH 7.0 and 25 degrees Celsius)


substrate_name: D-threitol (at pH 7.0 and 25 degrees Celsius)


substrate_name: xylitol (at pH 7.0 and 25 degrees Celsius)


substrate_name: NADP(+) (at pH 7.0 and 25 degrees Celsius)


substrate_name: NAD(+) (at pH 7.0 and 25 degrees Celsius)


substrate_name: 1,4-dibromo-2,3-butanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: 2,3-pentanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: 2,3-hexanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: 3,4-hexanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: 2,3-heptanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: isatin (at pH 7.0 and 25 degrees Celsius)


substrate_name: 1-phenyl-1,2-propanedione (at pH 7.0 and 25 degrees Celsius)


substrate_name: propioin (at pH 7.0 and 25 degrees Celsius)


substrate_name: methylglyoxal (at pH 7.0 and 25 degrees Celsius)


substrate_name: NADPH

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

assay_conditions_summary: pH 7; 298.15 K; cofactor NADPH


substrate_name: NADP+

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

substrate: NADP+


substrate_name: L-xylitol

substrate: L-xylitol


substrate_name: L-xylulose

substrate: L-xylulose


proof_lines: pH 7.0, 25°C, wild-type enzyme and mutant L143F


substrate_name: diacetyl

substrate: diacetyl


substrate_name: D-xylulose

substrate: D-xylulose


substrate_name: dihydroxyacetone

substrate: dihydroxyacetone


substrate_name: D-erythrose

substrate: D-erythrose


substrate_name: D-threitol

substrate: D-threitol


substrate_name: DL-glyceraldehyde

substrate: DL-glyceraldehyde


substrate_name: L-erythrulose

substrate: L-erythrulose


substrate_name: xylitol

substrate: xylitol


substrate_name: acetoin

substrate: acetoin


substrate_name: NAD+

assay_conditions_summary: pH 7; 298.15 K; cofactor NAD+

substrate: NAD+


substrate: NADPH


substrate_name: NADH

assay_conditions_summary: pH 7; 298.15 K; cofactor NADH

substrate: NADH


substrate_name: D-threose

substrate: D-threose


assay_conditions_summary: pH 7; 298.15 K; cofactor NADP+


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature D211S/I212R/D213N to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature D211S/I212R/S348T to reconstruct the assayed variant sequence.


proof_lines: or above, mutant D211S/I212R/D213N, pH 8.0, 25°C


proof_lines: or above, mutant D211S/I212R/S348T, pH 8.0, 25°C


assay_conditions_summary: pH 7; 298.15 K; buffer Evidence used: selected batch payload only

proof_lines: wild type enzyme, in 10 mM potassium phosphate buffer


substrate_name: D-sorbitol

smiles: C(C(C(C(C(CO)O)O)O)O)O

proof_lines:
wild-type, pH 9.6, 20°C
wild-type

substrate: D-sorbitol


substrate_name: L-arabinitol

substrate: L-arabinitol


substrate_name: L-arabitol

substrate: L-arabitol


substrate_name: D-allitol

substrate: D-allitol


substrate_name: L-tagatose

smiles: C(C(C(C(C(=O)CO)O)O)O)O

substrate: L-tagatose


smiles: C(C(C(C(=O)C(CO)O)O)O)O


substrate_name: D-psicose

substrate: D-psicose


organism: Streptococcus mutans

assay_conditions_summary: cofactor NADH


assay_conditions_summary: cofactor NAD+


substrate_name: D-mannitol 1-phosphate

smiles: C(C(C(C(C(COP(=O)(O)O)O)O)O)O)O

substrate: D-mannitol 1-phosphate


substrate_name: D-fructose 6-phosphate

substrate: D-fructose 6-phosphate


substrate_name: Unknown

km_display: Not reported

ki_unit: mM

proof_lines: competitive inhibition with respect to NADH, noncompetitive inhibition with respect to fructose 6-phosphate

proof_lines: strong inhibition of mannitol 1-phosphate oxidation

proof_lines: able to inhibit the forward reaction to 100%, competitive inhibition with respect to mannitol 1-phosphate, noncompetitive inhibition with respect to NAD+

substrate: Unknown


organism: Cryptococcus neoformans


substrate_name: ethanol

smiles: CCO

substrate: ethanol


organism: Gluconobacter sp. JX-05

assay_conditions_summary: pH 8.5; 303.15 K; cofactor NAD+

proof_lines: pH 8.5, 30°C, recombinant His-tagged enzyme, with NAD+


assay_conditions_summary: pH 5.5; 303.15 K; cofactor NADH

proof_lines: pH 5.5, 30°C, recombinant His-tagged enzyme, with NADH


substrate_name: D-arabitol

substrate: D-arabitol


substrate_name: D-xylulose

smiles: C(C(C(C(=O)CO)O)O)O

substrate: D-xylulose


organism: [Candida] sp. HA 167

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature E154C with inferred residue-numbering offset -4 to reconstruct the assayed variant sequence.


assay_conditions_summary: pH 9; 298.15 K; cofactor NAD+


organism: Deinococcus geothermalis

substrate_name: NADP+

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

assay_conditions_summary: pH 9; cofactor NADP+

proof_lines: substrate-binding loop chimera plus mutation D55N, pH 9.0, temperature not specified in the publication

substrate: NADP+


assay_conditions_summary: pH 9; cofactor NAD+

substrate: NAD+


proof_lines: wild-type, pH 9.0, temperature not specified in the publication


proof_lines: substrate-binding loop chimera, pH 9.0, temperature not specified in the publication


organism: Equus caballus


substrate_name: D-fructose

substrate: D-fructose


organism: Pseudomonas sp.

substrate: NADH


substrate_name: NAD+


substrate_name: D-galactitol

substrate: D-galactitol


substrate_name: D-tagatose

substrate: D-tagatose


organism: Gluconobacter oxydans


substrate_name: NADH


substrate_name: D-sorbitol

assay_conditions_summary: pH 6; 298.15 K; cofactor NADP+

proof_lines: pH 6.0, 25°C, with 2,6-dichlorophenolindophenol as electron acceptor

substrate: D-sorbitol


organism: Rattus norvegicus


substrate_name: D-xylitol

substrate: D-xylitol


substrate_name: D-ribulose

substrate: D-ribulose


organism: Klebsiella aerogenes


organism: Ectocarpus siliculosus

assay_conditions_summary: pH 9; 303.15 K; cofactor NAD+


assay_conditions_summary: pH 7; 303.15 K; cofactor NADH


substrate_name: ribitol

smiles: C(C(C(C(CO)O)O)O)O

substrate: ribitol


substrate_name: glycerol

smiles: C(C(CO)O)O

substrate: glycerol


organism: Caloglossa continua

proof_lines:
pH 7.0, 25°C
pH 7.0, 25°C, presence of 200 mM NaCl


smiles: C(C(C(C(C(=O)CO)O)O)O)OP(=O)(O)O


organism: Rasamsonia emersonii

substrate_name: xylitol

substrate: xylitol


organism: Ambrosiozyma monospora

substrate_name: D-arabinitol

proof_lines: pH 9.0, 30°C, purified recombinant enzyme

substrate: D-arabinitol


proof_lines: pH 7.0, 30°C, purified recombinant enzyme


organism: Rhizomucor pusillus

substrate_name: L-xylulose

proof_lines:
pH 7.0, 25°C, native enzyme
pH 7.0, 25°C, recombinant enzyme

substrate: L-xylulose


proof_lines:
pH 9.6, 25°C, recombinant enzyme
pH 9.6, 25°C, native enzyme


organism: Scheffersomyces stipitis


organism: Neurospora crassa


assay_conditions_summary: pH 8; 298.15 K; cofactor NAD+


organism: Aspergillus niger

assay_conditions_summary: pH 9.6; 303.15 K; cofactor NADH


organism: Penicillium chrysogenum

assay_conditions_summary: pH 7; 295.15 K; cofactor NAD+


substrate_name: L-arabinitol

substrate: L-arabinitol


assay_conditions_summary: pH 10


organism: Ovis aries


enzyme_display_name: alcohol dehydrogenase

organism: Saimiri sciureus

substrate_name: ethanol

enzyme: alcohol dehydrogenase

substrate: ethanol


substrate_name: butanol

smiles: CCCCO

substrate: butanol


substrate_name: methanol

smiles: CO

substrate: methanol


substrate_name: Pentanol

smiles: CCCCCO

substrate: Pentanol


organism: Natronomonas pharaonis

substrate_name: acetaldehyde

smiles: CC=O

assay_conditions_summary: pH 9

substrate: acetaldehyde


assay_conditions_summary: pH 7


organism: Sulfolobus sp.

substrate_name: benzylalcohol

substrate: benzylalcohol


organism: Haloferax volcanii

assay_conditions_summary: pH 6; cofactor NAD+

column_label: Table 2, row NAD(P)H, HvADH1 Km (mM) column reports 0.071 +/- 0.008; paper text states HvADH1 was exclusively NAD+ dependent.

raw_value_unit_evidence: Table 2 HvADH1 row NAD(P)H: Km = 0.071 +/- 0.008 mM

row_label: HvADH1 / NAD(P)H

table_label: Table 2

substrate: NADH


substrate_name: NAD+

assay_conditions_summary: pH 11; cofactor NAD+

column_label: Table 2, row NAD(P)+, HvADH1 Km (mM) column reports 0.29 +/- 0.02; paper text states HvADH1 was exclusively NAD+ dependent.

raw_value_unit_evidence: Table 2 HvADH1 row NAD(P)+: Km = 0.29 +/- 0.02 mM

row_label: HvADH1 / NAD(P)+

substrate: NAD+


organism: Fragaria x ananassa

smiles: CCCO


smiles: CC(C)O


smiles: CC(CO)O


substrate_name: benzyl alcohol

substrate: benzyl alcohol


assay_conditions_summary: pH 6; cofactor NADH

column_label: Table 2, row Acetaldehyde, HvADH1 Km (mM) column reports 28 +/- 3.

raw_value_unit_evidence: Table 2 HvADH1 row Acetaldehyde: Km = 28 +/- 3 mM

row_label: HvADH1 / Acetaldehyde


column_label: Table 2, row Ethanol, HvADH1 Km (mM) column reports 31 +/- 2.

raw_value_unit_evidence: Table 2 HvADH1 row Ethanol: Km = 31 +/- 2 mM

row_label: HvADH1 / Ethanol


organism: Homo sapiens

smiles: C(CCC=CCC=CCC=CCC=CCCCC(=O)O)CCO


proof_lines:
Table II
1-butanol
primary alcohols


proof_lines:
Table II
ethanol
primary alcohols


substrate_name: ethanol (at pH 10.0 and 25 degrees Celsius)

assay_conditions_summary: pH 10; buffer glycine

column_label: PDF Table 1 row Ethanol has ADH6 Km=28 mM under the table condition pH 10 at 25 C

raw_value_unit_evidence: Km raw_value=28 raw_unit=mM

row_label: Ethanol; enzyme ADH6

table_label: Table 1 Kinetic Properties of Alcohol Dehydrogenase Encoded by ADH6 and Stomach ADH Isozyme


substrate_name: 1-propanol (at pH 10.0 and 25 degrees Celsius)

column_label: PDF Table 1 row Propanol has ADH6 Km=3.2 mM under the table condition pH 10 at 25 C

raw_value_unit_evidence: Km raw_value=3.2 raw_unit=mM

row_label: Propanol; enzyme ADH6


substrate_name: benzyl alcohol (at pH 10.0 and 25 degrees Celsius)

column_label: PDF Table 1 row Benzyl Alcohol has ADH6 Km=0.12 mM under the table condition pH 10 at 25 C

raw_value_unit_evidence: Km raw_value=0.12 raw_unit=mM

row_label: Benzyl Alcohol; enzyme ADH6


substrate_name: all-trans-retinol

assay_conditions_summary: pH 7.4; cofactor NAD+

column_label: PMC full-text HTML for PMID 16787387; Table 1 'Kinetic constants of purified AKR, MDR and SDR enzymes with retinoids'; row All-trans-Retinol / Km (uM); ADH4 column=0.3+/-0.03.

raw_value_unit_evidence: PMID 16787387 Table 1 reports ADH4 / All-trans-Retinol: Km=0.3+/-0.03 uM and kcat=190+/-6 min^-1.

row_label: All-trans-Retinol; ADH4 column

table_label: Table 1

substrate: all-trans-retinol


assay_conditions_summary: pH 7.5; cofactor NAD+

column_label: UAB public PDF reprint of PMID 15369820; Table 1 'Kinetic constants of human alcohol dehydrogenases with retinoids'; row 4-Hydroxy-retinol / Km (uM); ADH4 column=15+/-3.

raw_value_unit_evidence: PMID 15369820 Table 1 reports ADH4 / 4-Hydroxy-retinol: Km=15+/-3 uM and kcat=2050+/-250 min^-1.

row_label: 4-Hydroxy-retinol; ADH4 column


column_label: UAB public PDF reprint of PMID 15369820; Table 1 'Kinetic constants of human alcohol dehydrogenases with retinoids'; row 3,4-Didehydroretinol / Km (uM); ADH4 column=28+/-7.

raw_value_unit_evidence: PMID 15369820 Table 1 reports ADH4 / 3,4-Didehydroretinol: Km=28+/-7 uM and kcat=150+/-14 min^-1.

row_label: 3,4-Didehydroretinol; ADH4 column


substrate_name: all-trans-retinal

assay_conditions_summary: pH 7.4; cofactor NADH

column_label: PMC full-text HTML for PMID 16787387; Table 1 'Kinetic constants of purified AKR, MDR and SDR enzymes with retinoids'; row All-trans-Retinaldehyde / Km (uM); ADH4 column=0.8+/-0.1.

raw_value_unit_evidence: PMID 16787387 Table 1 reports ADH4 / All-trans-Retinaldehyde: Km=0.8+/-0.1 uM and kcat=300+/-10 min^-1.

row_label: All-trans-Retinaldehyde; ADH4 column

substrate: all-trans-retinal


assay_conditions_summary: pH 7.5; cofactor NADH

column_label: UAB public PDF reprint of PMID 15369820; Table 1 'Kinetic constants of human alcohol dehydrogenases with retinoids'; row 4-Oxo-retinal / Km (uM); ADH4 column=17+/-4.

raw_value_unit_evidence: PMID 15369820 Table 1 reports ADH4 / 4-Oxo-retinal: Km=17+/-4 uM and kcat=1200+/-140 min^-1.

row_label: 4-Oxo-retinal; ADH4 column


column_label: UAB public PDF reprint of PMID 15369820; Table 1 'Kinetic constants of human alcohol dehydrogenases with retinoids'; row 3,4-Didehydroretinal / Km (uM); ADH4 column=26+/-6.

raw_value_unit_evidence: PMID 15369820 Table 1 reports ADH4 / 3,4-Didehydroretinal: Km=26+/-6 uM and kcat=73+/-5 min^-1.

row_label: 3,4-Didehydroretinal; ADH4 column


substrate_name: 10-OH-decanoic acid (with 0.1 M sodium phosphate at pH 7.5)

smiles: C(CCCCC(=O)O)CCCCO


substrate_name: hexanol (with 0.1 M sodium phosphate at pH 7.5)

smiles: CCCCCCO


substrate_name: hexanal (with 0.1 M sodium phosphate at pH 7.5)

smiles: CCCCCC=O


substrate_name: 2-hexenol (with 0.1 M sodium phosphate at pH 7.5)

smiles: CCCC=CCO


substrate_name: 2-hexenal (with 0.1 M sodium phosphate at pH 7.5)

smiles: CCCC=CC=O


substrate_name: 4-hydroxynonenal (with 0.1 M sodium phosphate at pH 7.5)

smiles: CCCCCC(C=CC=O)O


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: NAD(+)

assay_conditions_summary: cofactor NAD+

substrate: NAD(+)


substrate_name: ethanol

smiles: CCO


substrate_name: acetaldehyde


substrate_name: NADH

assay_conditions_summary: cofactor NADH


substrate_name: propanol

substrate: propanol


substrate_name: butanol


substrate_name: pentanol

proof_lines: pentanol butanol hexanol

substrate: pentanol


substrate_name: hexanol

proof_lines: hexanol pentanol butanol

substrate: hexanol


proof_lines: NAD+ ethanol acetaldehyde


proof_lines: ethanol NAD+ acetaldehyde


proof_lines: acetaldehyde NAD+ ethanol


proof_lines: NADH NAD+ ethanol


proof_lines: propanol butanol pentanol


proof_lines: butanol pentanol hexanol


proof_lines: pentanol hexanol butanol


proof_lines: 1-propanol propanol butanol


proof_lines: butan-1-ol butanol pentanol


substrate_name: butanal

smiles: CCCC=O

substrate: butanal


proof_lines:
alcohol dehydrogenase I
pentanol
butanol


proof_lines:
alcohol dehydrogenase I
hexanol
butanol


smiles: CCCCCCCO

assay_conditions_summary: pH 7.3; cofactor NAD+

column_label: Table IV; K_m (mM) block; Sc I row; Heptyl column; raw Km value 7.8 mM.

raw_value_unit_evidence: Table IV reports Sc I K_m for Heptyl alcohol = 7.8 mM.

row_label: Sc I wild-type Saccharomyces cerevisiae alcohol dehydrogenase I / P00330; K_m block; Heptyl column

table_label: PMID 8463307, Table IV: Kinetic constants for primary alcohols


smiles: CCCCCCCCO

column_label: Table IV; K_m (mM) block; Sc I row; Octyl column; raw Km value 5.3 mM.

raw_value_unit_evidence: Table IV reports Sc I K_m for Octyl alcohol = 5.3 mM.

row_label: Sc I wild-type Saccharomyces cerevisiae alcohol dehydrogenase I / P00330; K_m block; Octyl column


smiles: CCCCCCCCCO


smiles: CCC(C)O


substrate_name: isobutanol

smiles: CC(C)CO

column_label: Table V; K_m (mM) block; Sc I row; 2-Methyl-1-propanol column; raw Km value 25 mM.

raw_value_unit_evidence: Table V reports Sc I K_m for 2-methyl-1-propanol = 25 mM; 2-methyl-1-propanol is isobutanol.

row_label: Sc I wild-type Saccharomyces cerevisiae alcohol dehydrogenase I / P00330; K_m block; 2-Methyl-1-propanol column

table_label: PMID 8463307, Table V: Kinetic constants for secondary and branched alcohols

substrate: isobutanol


organism: Escherichia coli (strain K12)

substrate_name: acetaldehyde (for acetaldehyde dehydrogenase activity)

smiles: CC=O

assay_conditions_summary: pH 10; cofactor NADH

column_label: Table III; wild-type AdhE row; K_m acetaldehyde column; raw value 5.4 mM.

raw_value_unit_evidence: Table III reports wild-type AdhE K_m for acetaldehyde = 5.4 mM.

row_label: Wild-type AdhE row

table_label: PMID 10922373, Table III


substrate_name: ethanol (for ethanol dehydrogenase activity)

column_label: Table III; wild-type AdhE row; K_m ethanol column; raw value 240 mM.

raw_value_unit_evidence: Table III reports wild-type AdhE K_m for ethanol = 240 mM.


substrate_name: NAD

substrate: NAD


substrate: ethanol


substrate_name: S-(hydroxymethyl)glutathione (at pH 8)

assay_conditions_summary: pH 8

substrate: S-(hydroxymethyl)glutathione (at pH 8)


substrate_name: formaldehyde (at pH 8)

smiles: C=O

substrate: formaldehyde (at pH 8)


substrate_name: methylglyoxal (at pH 9.6)

smiles: CC(=O)C=O

substrate: methylglyoxal (at pH 9.6)


substrate_name: butanol (at pH 9.6)

smiles: CCCCO

substrate: butanol (at pH 9.6)


substrate_name: pentanol (at pH 9.6)

smiles: CCCCCO

substrate: pentanol (at pH 9.6)


substrate_name: hexanol (at pH 9.6)

smiles: CCCCCCO

substrate: hexanol (at pH 9.6)


substrate_name: octanol (at pH 9.6)

substrate: octanol (at pH 9.6)


substrate_name: allylalcohol (at pH 9.6)

smiles: C=CCO

substrate: allylalcohol (at pH 9.6)


substrate_name: glutathione (at pH 9.6)

smiles: C(CC(=O)NC(CS)C(=O)NCC(=O)O)C(C(=O)O)N

substrate: glutathione (at pH 9.6)


organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843)

substrate_name: NAD(+)

substrate: NAD(+)


substrate_name: ethanol

column_label: Table II -> row K_b, uM -> column S. pombe -> 14,000; footnote a maps K_b to the Michaelis constant for ethanol.

raw_value_unit_evidence: Km raw_value=14,000 raw_unit=uM

row_label: K_b, uM

table_label: Table II, Kinetic constants for yeast alcohol dehydrogenases


substrate_name: acetaldehyde

substrate: acetaldehyde


substrate_name: NADH

substrate: NADH


organism: Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM)

assay_conditions_summary: pH 9.5; cofactor NAD+

column_label: PMCID PMC1137609 scanned page 745, left column, Molecular characterization of EhADH2, K_m values paragraph; ethanol is listed in the ADH activity sentence.

raw_value_unit_evidence: Primary paper page 745 reports ADH K_m 80 mM for ethanol.

row_label: ADH activity; ethanol

table_label: Prose: Molecular characterization of EhADH2 / K_m values


assay_conditions_summary: pH 6.5; cofactor NAD+

column_label: PMCID PMC1137609 scanned page 745, left column, Molecular characterization of EhADH2, K_m values paragraph; acetaldehyde is listed in the ADH activity sentence.

raw_value_unit_evidence: Primary paper page 745 reports ADH K_m 0.15 mM for acetaldehyde.

row_label: ADH activity; acetaldehyde


substrate_name: acetyl-CoA

column_label: Km values for acetyl-CoA, acetaldehyde and ethanol were 0.015, 0.15 and 80 mM respectively in the presence of 0.2 mM NAD+.

raw_value_unit_evidence: Km raw_value=0.015 mM raw_unit=mM

row_label: Prose segment: acetyl-CoA Km

table_label: PubMed PMID 7980441 abstract

substrate: acetyl-CoA


substrate_name: NAD(+) (for ADH activity)

assay_conditions_summary: cofactor NADP+

substrate: NAD(+) (for ADH activity)


substrate_name: NADH (for ADH activity)

substrate: NADH (for ADH activity)


organism: Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337)

substrate_name: acetaldehyde (with NADH as cosubstrate)

substrate: acetaldehyde (with NADH as cosubstrate)


substrate_name: acetoin (with NADH as cosubstrate)

smiles: CC(C(=O)C)O

substrate: acetoin (with NADH as cosubstrate)


substrate_name: diacetyl (with NADH as cosubstrate)

smiles: CC(=O)C(=O)C

assay_conditions_summary: pH 5.8; buffer pH 5; cofactor NADH

column_label: PMID 6378632 PDF text lines 330-350; Table 3 row Diacetyl, substrate Km column, value 0.56 mM.

raw_value_unit_evidence: Table 3 row Diacetyl, substrate Km 0.56 mM.

row_label: Diacetyl

table_label: Table 3

substrate: diacetyl (with NADH as cosubstrate)


substrate_name: erythro-2,3-butanediol (with NAD as cosubstrate)

smiles: CC(C(C)O)O

assay_conditions_summary: pH 9.2; buffer pH 9; cofactor NAD+

column_label: PMID 6378632 PDF text lines 330-350; Table 3 row erythro-2,3-Butanediol, substrate Km column, value 18.20 mM.

raw_value_unit_evidence: Table 3 row erythro-2,3-Butanediol, substrate Km 18.20 mM.


substrate_name: ethanol (with NAD as cosubstrate)

smiles: CCO

column_label: PMID 6378632 PDF text lines 330-350; Table 3 row Ethanol, substrate Km column, value 5.00 mM.

normalized_value_unit_evidence: km=5.0 mM

raw_value_unit_evidence: Table 3 row Ethanol, substrate Km 5.00 mM.

row_label: Ethanol

substrate: ethanol (with NAD as cosubstrate)


substrate_name: NADH (with acetaldehyde as cosubstrate)

substrate: NADH (with acetaldehyde as cosubstrate)


substrate_name: NAD (with ethanol as cosubstrate)

substrate: NAD (with ethanol as cosubstrate)


substrate_name: NADPH (with acetaldehyde as cosubstrate)

assay_conditions_summary: cofactor NADPH


organism: Macaca mulatta

column_label: Table III; Ethanol row; MmADH alpha Km column 15 mM.

normalized_value_unit_evidence: Paper reports ethanol Km 15 mM.

raw_value_unit_evidence: Ethanol row; MmADH alpha Km 15 mM

row_label: Ethanol row under primary alcohols

table_label: Table III: Kinetic constants for primary branched-chain secondary alcohols

substrate: ethanol


smiles: CCCO

column_label: Table III; Propanol row; MmADH alpha Km column 2.0 mM.

normalized_value_unit_evidence: Paper reports propanol Km 2.0 mM.

raw_value_unit_evidence: Propanol row; MmADH alpha Km 2.0 mM

row_label: Propanol row under primary alcohols


column_label: Table III; Butanol row; MmADH alpha Km column 0.19 mM.

normalized_value_unit_evidence: Paper reports butanol Km 0.19 mM.

raw_value_unit_evidence: Butanol row; MmADH alpha Km 0.19 mM

row_label: Butanol row under primary alcohols


substrate_name: cyclohexanol

column_label: Table III; Cyclohexanol row; MmADH alpha Km column 0.024 mM.

normalized_value_unit_evidence: Paper reports cyclohexanol Km 0.024 mM.

raw_value_unit_evidence: Cyclohexanol row; MmADH alpha Km 0.024 mM

row_label: Cyclohexanol row under secondary alcohols

substrate: cyclohexanol


proof_lines:
R isomer of 2-butanol
stereoselective


smiles: CC(C)O

column_label: Table III; 2-Propanol row; MmADH alpha Km column 3.5 mM.

normalized_value_unit_evidence: Paper reports 2-propanol Km 3.5 mM.

raw_value_unit_evidence: 2-Propanol row; MmADH alpha Km 3.5 mM

row_label: 2-Propanol row under secondary alcohols


enzyme_display_name: alcohol dehydrogenase (NADP+)

organism: Pelophylax perezi

substrate_name: m-nitrobenzaldehyde

enzyme: alcohol dehydrogenase (NADP+)

substrate: m-nitrobenzaldehyde


substrate_name: all-trans-retinal

substrate: all-trans-retinal


substrate_name: octanal

smiles: CCCCCCCC=O

substrate: octanal


substrate_name: hexanal

smiles: CCCCCC=O

substrate: hexanal


substrate_name: p-nitrobenzaldehyde

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

proof_lines: Kinetic mechanism of sheep liver NADPH-dependent aldehyde reductase

substrate: p-nitrobenzaldehyde


smiles: CCCC=CC=O


substrate_name: pentanal

smiles: CCCCC=O

substrate: pentanal


substrate_name: Octanol

smiles: CCCCCCCCO

substrate: Octanol


substrate_name: Butanal

smiles: CCCC=O

substrate: Butanal


smiles: CCCC=CCO


substrate_name: Hexanol

substrate: Hexanol


substrate_name: D-glucuronate

substrate: D-glucuronate


substrate_name: acetaldehyde

smiles: CC=O

substrate: acetaldehyde


substrate_name: ethanol


smiles: CCC(C)O


substrate_name: cinnamaldehyde

substrate: cinnamaldehyde


substrate_name: veratraldehyde

substrate: veratraldehyde


smiles: CC(C)CC=O


substrate_name: NADPH

substrate: NADPH


substrate_name: cinnamyl alcohol

substrate: cinnamyl alcohol


substrate_name: phenylethanol

substrate: phenylethanol


substrate_name: pentanol

smiles: CCCCCO

substrate: pentanol


smiles: CC(C)CCO


substrate_name: NADP

substrate: NADP


organism: Rattus norvegicus


column_label: Table 3 row CBA; enzyme rAFAR1-1; KM column; value 0.7 +/- 0.2 uM.

normalized_value_unit_evidence: 2-carboxybenzaldehyde is CBA; 0.7 uM normalizes to 0.0007 mM.

raw_value_unit_evidence: Table 3 row CBA and enzyme rAFAR1-1 reports KM 0.7 +/- 0.2 uM.

row_label: rAFAR1-1 / CBA

table_label: Table 3: Kinetic properties of rat AFAR isoenzymes


substrate_name: succinic semialdehyde

smiles: C(CC(=O)O)C=O

column_label: Table 3 row SSA; enzyme rAFAR1-1; KM column; value 163 +/- 25 uM.

normalized_value_unit_evidence: SSA is succinic semialdehyde; 163 uM normalizes to 0.163 mM.

raw_value_unit_evidence: Table 3 row SSA and enzyme rAFAR1-1 reports KM 163 +/- 25 uM.

row_label: rAFAR1-1 / SSA

substrate: succinic semialdehyde


column_label: Table 3 row NADPH (with CBA); enzyme rAFAR1-1; KM column; value 1.66 +/- 0.19 uM.

normalized_value_unit_evidence: 1.66 uM normalizes to 0.00166 mM.

raw_value_unit_evidence: Table 3 row NADPH (with CBA) and enzyme rAFAR1-1 reports KM 1.66 +/- 0.19 uM.

row_label: rAFAR1-1 / NADPH (with CBA)


organism: Mus musculus

proof_lines:
D-glucuronate
L-gulonate
aldehyde reductase


organism: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

substrate_name: octanal

assay_conditions_summary: pH 7.3; cofactor NADPH

column_label: PMID 11257547 PDF page 5, Table 1, Octanal row, Ms-ADHC columns: Km 30 uM; kcat 37.8 s^-1; kcat/Km 1260 mM^-1 s^-1.

normalized_value_unit_evidence: Table 1 Octanal/Ms-ADHC: Km normalized_value=0.03 normalized_unit=mM.

raw_value_unit_evidence: Octanal/Ms-ADHC: Km raw_value=30 raw_unit=uM; kcat raw_value=37.8 raw_unit=s^-1; kcat/Km raw_value=1260 raw_unit=mM^-1*s^-1.

row_label: Octanal; Ms-ADHC column

table_label: Table 1. Substrate preference of purified M. smegmatis ADHC (PDF page 5 of 6; printed page 55)

substrate: octanal


assay_conditions_summary: pH 7.3; cofactor NADP+

column_label: PMID 11257547 PDF page 5, Table 1, Cinnamaldehyde row, Ms-ADHC columns: Km 40 uM; kcat 18.6 s^-1; kcat/Km 465 mM^-1 s^-1.

normalized_value_unit_evidence: Table 1 Cinnamaldehyde/Ms-ADHC: Km normalized_value=0.04 normalized_unit=mM.

raw_value_unit_evidence: Cinnamaldehyde/Ms-ADHC: Km raw_value=40 raw_unit=uM; kcat raw_value=18.6 raw_unit=s^-1; kcat/Km raw_value=465 raw_unit=mM^-1*s^-1.

row_label: Cinnamaldehyde; Ms-ADHC column


substrate_name: benzaldehyde

column_label: PMID 11257547 PDF page 5, Table 1, Benzaldehyde row, Ms-ADHC columns: Km 53 uM; kcat 32.1 s^-1; kcat/Km 605 mM^-1 s^-1.

normalized_value_unit_evidence: Table 1 Benzaldehyde/Ms-ADHC: Km normalized_value=0.053 normalized_unit=mM.

raw_value_unit_evidence: Benzaldehyde/Ms-ADHC: Km raw_value=53 raw_unit=uM; kcat raw_value=32.1 raw_unit=s^-1; kcat/Km raw_value=605 raw_unit=mM^-1*s^-1.

row_label: Benzaldehyde; Ms-ADHC column

substrate: benzaldehyde


enzyme_display_name: homoserine dehydrogenase

organism: Arabidopsis thaliana

substrate_name: aspartate for the aspartokinase activity (in the presence of 40 mM ATP)

assay_conditions_summary: cofactor ATP

enzyme: homoserine dehydrogenase


substrate_name: ATP for the aspartokinase activity (in the presence of 50 mM aspartate)


substrate_name: homoserine for the reverse reaction of the homoserine dehydrogenase activity (in the presence of 1 mM NADP)

smiles: C(CO)C(C(=O)O)N


substrate_name: aspartate semialdehyde for the forward reaction of the homoserine dehydrogenase activity (in the presence of NADPH)

smiles: C(C=O)C(C(=O)O)N


substrate_name: NADP for the reverse reaction of the homoserine dehydrogenase activity (in the presence of 50 mM homoserine)


substrate_name: aspartate for the aspartokinase activity (at pH 8.0, in the presence of 150 mM KCl, 20 mM MgCl(2), 200 uM NADPH and 20 mM ATP)

assay_conditions_summary: pH 8; cofactor ATP

proof_lines: AK activities in the presence of low concentrations of the substrates ATP and Asp


substrate_name: ATP for the aspartokinase activity (at pH 8.0, in the presence of 150 mM KCl, 20 mM MgCl(2), 200 uM NADPH and 50 mM aspartate)


substrate_name: aspartate semialdehyde for the forward reaction of the homoserine dehydrogenase activity (at pH 8.0, in the presence of 150 mM KCl and 200 uM NADPH)

column_label: Official JBC prose reports HSDH I Km for aspartate semialdehyde as 290 +/- 17 uM.

raw_value_unit_evidence: HSDH I Km for aspartate semialdehyde 290 +/- 17 uM.

row_label: HSDH I / aspartate semialdehyde

table_label: Official JBC full-text prose after TABLE ONE


organism: Bacillus subtilis (strain 168)

substrate_name: L-homoserine (at pH 9 and at 25 degrees Celsius)

assay_conditions_summary: pH 9; cofactor NADP+

column_label: Abstract reports L-homoserine Km = 35.08 +/- 2.91 mM, which does not exactly equal the current row value 35.0 mM.

raw_value_unit_evidence: Km raw_value=35.08 +/- 2.91 mM raw_unit=mM

row_label: Bacillus subtilis homoserine dehydrogenase / L-homoserine

table_label: PubMed XML abstract PMID 33046675


substrate_name: NADP+ (at pH 9 and at 25 degrees Celsius)

column_label: Abstract reports Km values for L-homoserine and NADP+ as 35.08 +/- 2.91 mM and 0.39 +/- 0.05 mM, respectively.

raw_value_unit_evidence: Km raw_value=0.39 +/- 0.05 mM raw_unit=mM

row_label: Bacillus subtilis homoserine dehydrogenase / NADP+


organism: Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)

substrate_name: NAD(+)

proof_lines:
homoserine dehydrogenase
Sulfolobus tokodaii
NAD

substrate: NAD(+)


organism: Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534)

substrate_name: L-aspartate 4-semialdehyde

substrate: L-aspartate 4-semialdehyde


substrate_name: NADPH


enzyme_display_name: (R,R)-butanediol dehydrogenase

organism: Saccharomyces cerevisiae

substrate_name: NADH

enzyme: (R,R)-butanediol dehydrogenase

substrate: NADH


substrate_name: NAD+

substrate: NAD+


smiles: CC(C(=O)C)O


smiles: CC(C(C)O)O


smiles: CC(=O)CO


smiles: CCC(CO)O

proof_lines:
Saccharomyces cerevisiae
YAL060W
2,3-butanediol dehydrogenase


substrate_name: acetaldehyde (with NADH as cosubstrate)

proof_lines: multifunctional NAD(P)-linked alcohol dehydrogenase

substrate: acetaldehyde (with NADH as cosubstrate)


substrate_name: acetoin (with NADH as cosubstrate)

substrate: acetoin (with NADH as cosubstrate)


substrate_name: diacetyl (with NADH as cosubstrate)

smiles: CC(=O)C(=O)C

substrate: diacetyl (with NADH as cosubstrate)


substrate_name: erythro-2,3-butanediol (with NAD as cosubstrate)


substrate_name: ethanol (with NAD as cosubstrate)

smiles: CCO

substrate: ethanol (with NAD as cosubstrate)


substrate_name: NADH (with acetaldehyde as cosubstrate)

substrate: NADH (with acetaldehyde as cosubstrate)


substrate_name: NAD (with ethanol as cosubstrate)

substrate: NAD (with ethanol as cosubstrate)


substrate_name: NADPH (with acetaldehyde as cosubstrate)


enzyme_display_name: glycerol dehydrogenase

organism: Geobacillus stearothermophilus

substrate_name: glycerol

smiles: C(C(CO)O)O

enzyme: glycerol dehydrogenase

substrate: glycerol


organism: Citrobacter freundii

column_label: Abstract reports apparent Kms of glycerol dehydrogenase for glycerol and NAD+ were 1.27 mM and 57 microM, respectively.

raw_value_unit_evidence: Km raw_value=1.27 mM raw_unit=mM

row_label: Citrobacter glycerol dehydrogenase / glycerol

table_label: PubMed XML abstract PMID 7635824


raw_value_unit_evidence: Km raw_value=57 microM raw_unit=microM

row_label: Citrobacter glycerol dehydrogenase / NAD+


organism: Oryctolagus cuniculus

substrate_name: alpha-glycerophosphate

proof_lines:
mastoidectomy
contralateral hearing loss
sensorineural hearing loss

substrate: alpha-glycerophosphate


substrate_name: glycerol 3-phosphate

smiles: C(C(COP(=O)(O)O)O)O

proof_lines: rabbit muscle L-glycerol 3-phosphate dehydrogenase

substrate: glycerol 3-phosphate


organism: Homo sapiens

assay_conditions_summary: pH 9.5; buffer hydrazine-glycine

normalized_value_unit_evidence: Km normalized_value=0.092 normalized_unit=mM from 92 uM.

raw_value_unit_evidence: Table 2 row This paper / human liver / cytosolic reports K m glycerol-3-phosphate = 92 uM, normalized to 0.092 mM. Raw value 92 uM.

row_label: This paper / human liver / cytosolic

table_label: Table 2, kinetic constants of glycerol-3-phosphate dehydrogenases


substrate_name: NAD

normalized_value_unit_evidence: Km normalized_value=0.14 normalized_unit=mM from 140 uM.

raw_value_unit_evidence: Table 2 row This paper / human liver / cytosolic reports K m NAD = 140 uM, normalized to 0.14 mM. Raw value 140 uM.

substrate: NAD


substrate_name: NADP

proof_lines:
NADP
Km
human liver cytosolic

substrate: NADP


substrate_name: glycerone phosphate

smiles: C(C(=O)COP(=O)(O)O)O

proof_lines:
AtGPDHp
DHAP
glycerone phosphate

substrate: glycerone phosphate


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)


substrate_name: dihydroxyacetone phosphate

substrate: dihydroxyacetone phosphate


enzyme_display_name: D-xylulose reductase

organism: Morganella morganii

substrate_name: xylitol

smiles: C(C(C(C(CO)O)O)O)O

enzyme: D-xylulose reductase

substrate: xylitol


substrate_name: sorbitol

smiles: C(C(C(C(C(CO)O)O)O)O)O

column_label: [{'metric': 'km', 'evidence_type': 'prose', 'table_label': 'Kinetic properties prose', 'row_label': 'sorbitol', 'column_label': 'K_m', 'raw_value': '1.54', 'raw_unit': 'mM', 'normalized_value': 1.54, 'normalized_unit': 'mM', 'condition': 'oxidative direction; same Q64442/PMID source group; paper EC label 1.1.1.14'}]

normalized_value_unit_evidence: sorbitol K_m 1.54 mM.

raw_value_unit_evidence: K_m for sorbitol and NAD are 1.54 mM and 58.8 uM, respectively.

row_label: sorbitol

table_label: Kinetic properties prose

substrate: sorbitol


substrate_name: NAD(+)

column_label: [{'metric': 'km', 'evidence_type': 'prose', 'table_label': 'Kinetic properties prose', 'row_label': 'NAD+', 'column_label': 'K_m', 'raw_value': '58.8', 'raw_unit': 'uM', 'normalized_value': 0.0588, 'normalized_unit': 'mM', 'condition': 'oxidative direction; same Q64442/PMID source group; paper EC label 1.1.1.14'}]

row_label: NAD+

substrate: NAD(+)


substrate_name: fructose

column_label: [{'metric': 'km', 'evidence_type': 'prose', 'table_label': 'Kinetic properties prose', 'row_label': 'fructose', 'column_label': 'K_m', 'raw_value': '154', 'raw_unit': 'mM', 'normalized_value': 154.0, 'normalized_unit': 'mM', 'condition': 'reductive direction; same Q64442/PMID source group; paper EC label 1.1.1.14'}]

normalized_value_unit_evidence: fructose K_m 154 mM.

raw_value_unit_evidence: K_m for fructose and NADH are 154 mM and 15 uM, respectively.

row_label: fructose

substrate: fructose


substrate_name: NADH

column_label: [{'metric': 'km', 'evidence_type': 'prose', 'table_label': 'Kinetic properties prose', 'row_label': 'NADH', 'column_label': 'K_m', 'raw_value': '15', 'raw_unit': 'uM', 'normalized_value': 0.015, 'normalized_unit': 'mM', 'condition': 'reductive direction; same Q64442/PMID source group; paper EC label 1.1.1.14'}]

normalized_value_unit_evidence: NADH K_m 15 uM = 0.015 mM.

row_label: NADH

substrate: NADH


substrate_name: D-sorbitol

substrate: D-sorbitol


substrate_name: D-xylose

substrate: D-xylose


assay_conditions_summary: pH 7; cofactor NADH

column_label: Metric label Michaelis constant/Km; row NADH; column value 240 microM; condition pH 7.0

raw_value_unit_evidence: NADH 240 microM

row_label: Michaelis constants list; NADH

table_label: Abstract


assay_conditions_summary: pH 9

column_label: Metric label Michaelis constant/Km; row xylitol; column value 25 mM; condition pH 9.0 with NAD

normalized_value_unit_evidence: 25 mM

raw_value_unit_evidence: xylitol 25 mM

row_label: Michaelis constants list; xylitol


column_label: Metric label Michaelis constant/Km; row NAD; column value 100 microM; condition pH 9.0

raw_value_unit_evidence: NAD 100 microM

row_label: Michaelis constants list; NAD


organism: Aspergillus niger

substrate_name: NADPH

proof_lines: NADPH-dependent L-xylulose xylitol

substrate: NADPH


substrate_name: NADP+

proof_lines:
xylitol:NADP 4-oxidoreductase
NADP(H)

substrate: NADP+


proof_lines: xylitol L-xylulose NADP(H)


organism: Gallus gallus

substrate_name: D-erythrulose

smiles: C(C(C(=O)CO)O)O

column_label: PMID 7358641 / DOI 10.1093/oxfordjournals.jbchem.a132751, abstract prose, D-erythrulose reductase from chicken liver, D-erythrulose apparent Km = 0.38 mM.

raw_value_unit_evidence: Primary abstract reports that the enzyme was highly specific to D-erythrulose with an apparent Km of 0.38 mM.

row_label: D-erythrulose reductase / D-erythrulose

table_label: PubMed/Europe PMC abstract prose

substrate: D-erythrulose


column_label: PMID 7358641 / DOI 10.1093/oxfordjournals.jbchem.a132751, abstract prose, D-erythrulose reductase from chicken liver, NADH Km = 67 uM.

raw_value_unit_evidence: Primary abstract reports Km values for NADH and NADPH of 67 uM and 7.9 uM, respectively; PubMed/Europe PMC render the microM symbol as 'micrometers'.

row_label: D-erythrulose reductase / NADH


column_label: PMID 7358641 / DOI 10.1093/oxfordjournals.jbchem.a132751, abstract prose, D-erythrulose reductase from chicken liver, NADPH Km = 7.9 uM.

row_label: D-erythrulose reductase / NADPH


organism: Klebsiella aerogenes

assay_conditions_summary: cofactor NADH


substrate_name: NAD+

assay_conditions_summary: cofactor NAD+

substrate: NAD+


substrate_name: D-xylulose

smiles: C(C(C(C(=O)CO)O)O)O

substrate: D-xylulose


substrate_name: D-arabinitol

substrate: D-arabinitol


substrate_name: D-mannitol

substrate: D-mannitol


organism: Hypocrea jecorina

substrate_name: L-arabinitol (at pH 7)

assay_conditions_summary: pH 7

substrate: L-arabinitol (at pH 7)


substrate_name: D-talitol (at pH 8.6)

substrate: D-talitol (at pH 8.6)


substrate_name: L-xylo-3-hexulose (at pH 8.6)

smiles: C(C(C(C(=O)C(CO)O)O)O)O

substrate: L-xylo-3-hexulose (at pH 8.6)


substrate_name: D-fructose (at pH 8.6)

substrate: D-fructose (at pH 8.6)


substrate_name: L-tagatose (at pH 8.6)

smiles: C(C(C(C(C(=O)CO)O)O)O)O

substrate: L-tagatose (at pH 8.6)


substrate_name: NAD (at pH 7)

substrate: NAD (at pH 7)


organism: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

substrate_name: L-arabinitol (at pH 8)

assay_conditions_summary: pH 8

substrate: L-arabinitol (at pH 8)


substrate_name: NAD (at pH 8)

substrate: NAD (at pH 8)


organism: Aspergillus niger (strain ATCC MYA-4892 / CBS 513.88 / FGSC A1513)

substrate_name: L-arabinitol (at pH 9.6)

proof_lines:
Aspergillus niger
L-arabinose catabolic pathway
L-arabitol dehydrogenase

substrate: L-arabinitol (at pH 9.6)


substrate_name: L-xylulose (at pH 9.6)

substrate: L-xylulose (at pH 9.6)


substrate_name: NAD (at pH 9.6)

substrate: NAD (at pH 9.6)


substrate_name: NADH (at pH 9.6)

assay_conditions_summary: pH 9.6; cofactor NADH

substrate: NADH (at pH 9.6)


organism: Rasamsonia emersonii

substrate_name: L-arabinitol

substrate: L-arabinitol


substrate_name: D-sorbitol

substrate: D-sorbitol


substrate_name: NAD

substrate: NAD


organism: Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255)


organism: Cereibacter sphaeroides

substrate_name: NADH

substrate: NADH


substrate_name: D-galactitol

substrate: D-galactitol


substrate_name: D-tagatose

substrate: D-tagatose


organism: Bacillus subtilis (strain 168)


substrate_name: xylitol

substrate: xylitol


organism: Mus musculus

substrate_name: sorbitol

assay_conditions_summary: pH 9; cofactor NAD+

column_label: [{'metric': 'km', 'evidence_type': 'prose', 'table_label': 'Kinetic properties prose', 'row_label': 'sorbitol', 'column_label': 'K_m', 'raw_value': '1.54', 'raw_unit': 'mM', 'normalized_value': 1.54, 'normalized_unit': 'mM', 'condition': 'oxidative direction; 30 C; Tris-glycine pH 9.0'}]

substrate: sorbitol


substrate_name: NAD(+)

column_label: [{'metric': 'km', 'evidence_type': 'prose', 'table_label': 'Kinetic properties prose', 'row_label': 'NAD+', 'column_label': 'K_m', 'raw_value': '58.8', 'raw_unit': 'uM', 'normalized_value': 0.0588, 'normalized_unit': 'mM', 'condition': 'oxidative direction; 30 C; Tris-glycine pH 9.0'}]

substrate: NAD(+)


substrate_name: fructose

assay_conditions_summary: pH 7.5; cofactor NADH

column_label: [{'metric': 'km', 'evidence_type': 'prose', 'table_label': 'Kinetic properties prose', 'row_label': 'fructose', 'column_label': 'K_m', 'raw_value': '154', 'raw_unit': 'mM', 'normalized_value': 154.0, 'normalized_unit': 'mM', 'condition': 'reductive direction; 30 C; Tris-HCl pH 7.5'}]

substrate: fructose


assay_conditions_summary: pH 7.5; cofactor NAD+

column_label: [{'metric': 'km', 'evidence_type': 'prose', 'table_label': 'Kinetic properties prose', 'row_label': 'NADH', 'column_label': 'K_m', 'raw_value': '15', 'raw_unit': 'uM', 'normalized_value': 0.015, 'normalized_unit': 'mM', 'condition': 'reductive direction; 30 C; Tris-HCl pH 7.5'}]


substrate_name: NAD(+) (in the presence of D-sorbitol)

assay_conditions_summary: cofactor NADPH

substrate: NAD(+) (in the presence of D-sorbitol)


enzyme_display_name: D-iditol 2-dehydrogenase

organism: Ambrosiozyma monospora

substrate_name: L-xylulose

proof_lines:
NADH-dependent L-xylulose reductase
L-xylulose
xylitol

enzyme: D-iditol 2-dehydrogenase

substrate: L-xylulose


substrate_name: D-arabinitol

proof_lines:
D-ribulose
D-arabinitol
L-xylulose reductase

substrate: D-arabinitol


proof_lines: D-glucitol sorbitol D-fructose


proof_lines: NAD+ D-glucitol sorbitol


substrate_name: D-fructose

proof_lines: D-fructose NADH D-tagatose

substrate: D-fructose


substrate_name: NADH (in the presence of D-fructose)

proof_lines: NADH D-fructose D-tagatose

substrate: NADH (in the presence of D-fructose)


organism: Brochothrix thermosphacta

substrate_name: NAD+

substrate: NAD+


organism: Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)

substrate_name: D-mannitol 1-phosphate

smiles: C(C(C(C(C(COP(=O)(O)O)O)O)O)O)O

assay_conditions_summary: pH 7.1; cofactor NAD+

column_label: Table 2 row Man-ol1P column Ksubstrate (mM)=0.23+/-0.02.

normalized_value_unit_evidence: Km normalized_value=0.23 mM

row_label: Man-ol1P / Ksubstrate

table_label: Table 2

substrate: D-mannitol 1-phosphate


substrate_name: D-fructose 6-phosphate

assay_conditions_summary: pH 7.1; cofactor NADH

column_label: Table 2 row Fru6P column Ksubstrate (mM)=2.1+/-0.2.

normalized_value_unit_evidence: Km normalized_value=2.1 mM

row_label: Fru6P / Ksubstrate

substrate: D-fructose 6-phosphate


column_label: Table 2 row Fru6P column Kcofactor (mM)=0.016+/-0.001.

normalized_value_unit_evidence: Km normalized_value=0.016 mM

raw_value_unit_evidence: Kcofactor raw_value=0.016+/-0.001 raw_unit=mM for NADH in Fru6P reduction

row_label: Fru6P / Kcofactor


column_label: Table 2 row Man-ol1P column Kcofactor (mM)=0.75+/-0.09.

normalized_value_unit_evidence: Km normalized_value=0.75 mM

raw_value_unit_evidence: Kcofactor raw_value=0.75+/-0.09 raw_unit=mM for NAD+ in Man-ol1P oxidation

row_label: Man-ol1P / Kcofactor


column_label: Table III row M-1-P; column Varied substrate K_m (mM); fixed substrate NAD (8 mM)

table_label: Table III


column_label: Table III row F-6-P; column Varied substrate K_m (mM); fixed substrate NADH (0.1 mM)


column_label: Table III row NADH; column Varied substrate K_m (mM); fixed substrate F-6-P (3 mM)


column_label: Table III row NAD; column Varied substrate K_m (mM); fixed substrate M-1-P (0.5 mM)


organism: Solanum lycopersicum

substrate_name: D-sorbitol

substrate: D-sorbitol


organism: Priestia megaterium


organism: Bacillus subtilis


substrate_name: D-xylitol

substrate: D-xylitol


organism: Aspergillus niger

substrate_name: L-arabitol

proof_lines: pH and temperature not specified in the publication

substrate: L-arabitol


substrate_name: galactitol

proof_lines: about, pH and temperature not specified in the publication

substrate: galactitol


organism: Malus domestica


organism: Trichoderma reesei


organism: Escherichia coli


substrate_name: hexitol phosphate

substrate: hexitol phosphate


organism: Homo sapiens

substrate_name: Unknown

proof_lines:
competitive with respect to xylitol
uncompetitive with respect to NADPH

substrate: Unknown


organism: Rattus norvegicus


organism: Rhizobium leguminosarum


organism: Aspergillus fumigatus

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature K213A to reconstruct the assayed variant sequence.


substrate_name: NADH

assay_conditions_summary: pH 10; 298.15 K; cofactor NADH

proof_lines:
pH 10.0, 25°C, recombinant enzyme
pH 7.1, 25°C, recombinant enzyme

substrate: NADH


organism: Saccharolobus solfataricus

substrate_name: trans-cinnamaldehyde

sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature W95L to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 10.5; buffer 0.1 M glycine-NaOH

proof_lines: Role of tryptophan 95 in substrate specificity and structural stability of Sulfolobus solfataricus alcohol dehydrogenase

substrate: trans-cinnamaldehyde


substrate_name: benzyl alcohol

substrate: benzyl alcohol


substrate_name: Butyraldehyde

smiles: CCCC=O

substrate: Butyraldehyde


substrate_name: benzaldehyde

substrate: benzaldehyde


substrate_name: 4-methoxybenzyl alcohol

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

substrate: 4-methoxybenzyl alcohol


smiles: CCCCCCO


smiles: CCCCCCCO


smiles: CCCCCO


substrate_name: Isobutyraldehyde

smiles: CC(C)C=O

substrate: Isobutyraldehyde


smiles: CCOCCO

assay_conditions_summary: pH 10.5; 338.15 K; buffer 0.1 M glycine-NaOH; cofactor NAD+

column_label: Table 2: Kinetic constants for wild-type and mutant SsADHs (Extremophiles 2009, 13:751-761), Wild-type column group, substrate row '2-Ethoxyethanol'; column 'kcat (s-1)' = 1.6; column 'Km (mM)' = 0.25; column 'kcat/Km (s-1 mM-1)' = 6.4.

normalized_value_unit_evidence: kcat: printed Table 2 cell 1.6 s-1; the printed unit is already s^(-1), no unit conversion applies; the paper value is 1.6 s^(-1); the exported value is 1.6 s^(-1). Km: printed Table 2 cell 0.25 mM; the printed unit is already mM, no unit conversion applies; the paper value is 0.25 mM; the exported value is 0.25 mM. kcat/Km: printed Table 2 cell 6.4 s-1 mM-1; s-1 mM-1 is the canonical s^(-1)*mM^(-1), no unit conversion applies; the paper value is 6.4 s^(-1)*mM^(-1); the exported value is 6.4 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, substrate row '2-Ethoxyethanol', Wild-type column group: kcat = 1.6 s-1; Km = 0.25 mM; printed kcat/Km = 6.4 s-1 mM-1.

row_label: Table 2, Wild-type column group, substrate row '2-Ethoxyethanol'


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature W95L/N249Y to reconstruct the assayed variant sequence.

smiles: CCC(C)O


smiles: CCCC(C)O


smiles: CCCO


substrate_name: ethanol

smiles: CCO

substrate: ethanol


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P39462 and applied mutation signature W95L/N249Y to reconstruct the assayed variant sequence.

column_label: Table 2: Kinetic constants for wild-type and mutant SsADHs (Extremophiles 2009, 13:751-761), Trp95Leu/Asn249Tyr column group, substrate row '1-Heptanol'; column 'kcat (s-1)' = 11.9; column 'Km (mM)' = 0.67; column 'kcat/Km (s-1 mM-1)' = 17.7.

normalized_value_unit_evidence: kcat: printed Table 2 cell 11.9 s-1; the printed unit is already s^(-1), no unit conversion applies; the paper value is 11.9 s^(-1); the exported value is 11.9 s^(-1). Km: printed Table 2 cell 0.67 mM; the printed unit is already mM, no unit conversion applies; the paper value is 0.67 mM; the exported value is 0.67 mM. kcat/Km: printed Table 2 cell 17.7 s-1 mM-1; s-1 mM-1 is the canonical s^(-1)*mM^(-1), no unit conversion applies; the paper value is 17.7 s^(-1)*mM^(-1); the exported value is 17.7612 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, substrate row '1-Heptanol', Trp95Leu/Asn249Tyr column group: kcat = 11.9 s-1; Km = 0.67 mM; printed kcat/Km = 17.7 s-1 mM-1.

row_label: Table 2, Trp95Leu/Asn249Tyr column group, substrate row '1-Heptanol'


substrate_name: Cyclohexanol

column_label: Table 2: Kinetic constants for wild-type and mutant SsADHs (Extremophiles 2009, 13:751-761), Trp95Leu/Asn249Tyr column group, substrate row 'Cyclohexanol'; column 'kcat (s-1)' = 12.4; column 'Km (mM)' = 4.5; column 'kcat/Km (s-1 mM-1)' = 2.7.

normalized_value_unit_evidence: kcat: printed Table 2 cell 12.4 s-1; the printed unit is already s^(-1), no unit conversion applies; the paper value is 12.4 s^(-1); the exported value is 12.4 s^(-1). Km: printed Table 2 cell 4.5 mM; the printed unit is already mM, no unit conversion applies; the paper value is 4.5 mM; the exported value is 4.5 mM. kcat/Km: printed Table 2 cell 2.7 s-1 mM-1; s-1 mM-1 is the canonical s^(-1)*mM^(-1), no unit conversion applies; the paper value is 2.7 s^(-1)*mM^(-1); the exported value is 2.75556 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, substrate row 'Cyclohexanol', Trp95Leu/Asn249Tyr column group: kcat = 12.4 s-1; Km = 4.5 mM; printed kcat/Km = 2.7 s-1 mM-1.

row_label: Table 2, Trp95Leu/Asn249Tyr column group, substrate row 'Cyclohexanol'

substrate: Cyclohexanol


smiles: CCCCO


column_label: Table 2: Kinetic constants for wild-type and mutant SsADHs (Extremophiles 2009, 13:751-761), Trp95Leu/Asn249Tyr column group, substrate row '1-Pentanol'; column 'kcat (s-1)' = 16.3; column 'Km (mM)' = 1.9; column 'kcat/Km (s-1 mM-1)' = 8.5.

normalized_value_unit_evidence: kcat: printed Table 2 cell 16.3 s-1; the printed unit is already s^(-1), no unit conversion applies; the paper value is 16.3 s^(-1); the exported value is 16.3 s^(-1). Km: printed Table 2 cell 1.9 mM; the printed unit is already mM, no unit conversion applies; the paper value is 1.9 mM; the exported value is 1.9 mM. kcat/Km: printed Table 2 cell 8.5 s-1 mM-1; s-1 mM-1 is the canonical s^(-1)*mM^(-1), no unit conversion applies; the paper value is 8.5 s^(-1)*mM^(-1); the exported value is 8.57895 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, substrate row '1-Pentanol', Trp95Leu/Asn249Tyr column group: kcat = 16.3 s-1; Km = 1.9 mM; printed kcat/Km = 8.5 s-1 mM-1.

row_label: Table 2, Trp95Leu/Asn249Tyr column group, substrate row '1-Pentanol'


column_label: Table 2: Kinetic constants for wild-type and mutant SsADHs (Extremophiles 2009, 13:751-761), Trp95Leu/Asn249Tyr column group, substrate row 'Benzyl alcohol'; column 'kcat (s-1)' = 20.0; column 'Km (mM)' = 3.8; column 'kcat/Km (s-1 mM-1)' = 5.2.

normalized_value_unit_evidence: kcat: printed Table 2 cell 20.0 s-1; the printed unit is already s^(-1), no unit conversion applies; the paper value is 20 s^(-1); the exported value is 20 s^(-1). Km: printed Table 2 cell 3.8 mM; the printed unit is already mM, no unit conversion applies; the paper value is 3.8 mM; the exported value is 3.8 mM. kcat/Km: printed Table 2 cell 5.2 s-1 mM-1; s-1 mM-1 is the canonical s^(-1)*mM^(-1), no unit conversion applies; the paper value is 5.2 s^(-1)*mM^(-1); the exported value is 5.26316 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 2, substrate row 'Benzyl alcohol', Trp95Leu/Asn249Tyr column group: kcat = 20.0 s-1; Km = 3.8 mM; printed kcat/Km = 5.2 s-1 mM-1.

row_label: Table 2, Trp95Leu/Asn249Tyr column group, substrate row 'Benzyl alcohol'


substrate_name: NAD+

substrate: NAD+


organism: Pyrococcus furiosus

substrate_name: NADP+

assay_conditions_summary: pH 8.8; cofactor NADP+

proof_lines:
monogenean taxonomy
classification of monogeneans
morphology of chitinoid formations

substrate: NADP+