proof_lines: mutant T165A, 55°C, pH not specified in the publication


organism: Aquifex aeolicus

substrate_name: S-adenosyl-L-methionine

source_db: brenda

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

protein_accession_database: UniProt

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution

proof_lines: mutant H104A, 55°C, pH not specified in the publication

substrate: S-adenosyl-L-methionine

source: brenda


proof_lines: mutant R108A, 55°C, pH not specified in the publication


proof_lines: mutant D97A, 55°C, pH not specified in the publication


proof_lines: mutant Y95A, 55°C, pH not specified in the publication


organism: Escherichia coli

substrate_name: guanine46 in m5U54-modified tRNAPhe

km_display: Not reported

mutation_signature: Unknown

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: single turnover apparent rate, pH 7.5, 37°C

substrate: guanine46 in m5U54-modified tRNAPhe


organism: Saccharomyces cerevisiae

substrate_name: guanine46 in tRNA

mutation_type: deletion

proof_lines: pH 7.5, 30°C, tRNA variant with deletion of the anticodon-arm

substrate: guanine46 in tRNA


proof_lines: pH 7.5, 30°C, tRNA variant with deletion of the aminoacyl-stem


enzyme_display_name: iodide peroxidase

organism: Homo sapiens

substrate_name: guaiacol

proof_lines: wild-type, pH 7.4, temperature not specified in the publication

enzyme: iodide peroxidase

substrate: guaiacol


proof_lines: mutant P499L, pH 7.4, temperature not specified in the publication


proof_lines: mutant G387R, pH 7.4, temperature not specified in the publication


proof_lines: mutant C808R, pH 7.4, temperature not specified in the publication


proof_lines: mutant D74A, 55°C, pH not specified in the publication


proof_lines: mutant K101A, 55°C, pH not specified in the publication


proof_lines: mutant R107A, 55°C, pH not specified in the publication


proof_lines: mutant Y167A, 55°C, pH not specified in the publication


proof_lines: mutant T132C, 55°C, pH not specified in the publication


proof_lines: mutant E47A, 55°C, pH not specified in the publication


proof_lines: mutant T132A, 55°C, pH not specified in the publication


proof_lines: pH 7.5, 30°C, tRNA variant with substitution of C48 by A

proof_lines: pH 7.5, 30°C, tRNA variant with disruption of C13-G22-G46 tertiary base pair

proof_lines: pH 7.5, 30°C, tRNA variant with disruption of tertiary base pairs between D- and T-arms


organism: Cricetulus griseus


substrate_name: UDP-GlcA

substrate: UDP-GlcA


substrate_name: UDP-GlcNAc

substrate: UDP-GlcNAc


enzyme_display_name: mogroside IE synthase

organism: Siraitia grosvenorii

substrate_name: UDP-alpha-D-glucose

enzyme: mogroside IE synthase

substrate: UDP-alpha-D-glucose


source_db: skid

source_license: CC BY-NC-ND 4.0

source: skid


substrate_name: mogrol

substrate: mogrol


organism: Staphylococcus aureus

substrate_name: RNA(n)

substrate: RNA(n)


organism: Staphylococcus epidermidis

substrate_name: ADP

assay_conditions_summary: pH 7.5; 299.15 K; cofactor ADP

proof_lines:
polyadenylation reaction, pH 7.5, 26°C
phosphorolysis reaction, pH 8.2, 37°C

substrate: ADP


enzyme_display_name: chondroitin 6-sulfotransferase

enzyme: chondroitin 6-sulfotransferase


organism: Mus musculus

substrate_name: 3'-phosphoadenylyl sulfate

proof_lines: pH 6.8, 37°C, recombinant mutant N457S, with chondroitin

substrate: 3'-phosphoadenylyl sulfate


proof_lines: pH 6.8, 37°C, recombinant mutant N413S, with keratan sulfate

proof_lines: pH 6.8, 37°C, recombinant mutant N413S, with chondroitin


proof_lines: pH 6.8, 37°C, recombinant mutant N250S, with chondroitin


proof_lines: pH 6.8, 37°C, recombinant wild-type enzyme, with chondroitin

proof_lines: pH 6.8, 37°C, recombinant wild-type enzyme, with keratan sulfate


enzyme_display_name: poly(ADP-ribose) glycohydrolase

organism: Deinococcus radiodurans

substrate_name: Poly(ADP-ribose)

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ADP

proof_lines: pH 7.5, 37°C, recombinant wild-type enzyme

enzyme: poly(ADP-ribose) glycohydrolase

substrate: Poly(ADP-ribose)


assay_conditions_summary: pH 7.2; 310.15 K; cofactor ADP

proof_lines: purified recombinant catalytic enzyme domain, in 50 mM KPO4 (pH 7.2), at 37°C


substrate_name: Alpha-NAD+

assay_conditions_summary: pH 7; 303.15 K; cofactor NAD+

substrate: Alpha-NAD+


enzyme_display_name: beta-galactofuranosidase

organism: Aspergillus nidulans

substrate_name: 4-nitrophenyl beta-D-galactofuranoside

enzyme: beta-galactofuranosidase

substrate: 4-nitrophenyl beta-D-galactofuranoside


enzyme_display_name: arginine deiminase

organism: Mycoplasma hominis

substrate_name: H2O; L-Arginine

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

enzyme: arginine deiminase

substrate: H2O; L-Arginine


organism: Pseudomonas aeruginosa


enzyme_display_name: aspartate racemase

organism: Acropora millepora

substrate_name: D-Aspartate

source_db: oed

enzyme: aspartate racemase

substrate: D-Aspartate

source: oed


organism: Bifidobacterium bifidum

substrate_name: L-Aspartate

substrate: L-Aspartate


organism: Crassostrea gigas


organism: Penaeus monodon


organism: Picrophilus torridus


organism: Pyrococcus horikoshii


organism: Aspergillus niger


organism: Streptomyces sp. JHA19


organism: Streptomyces sp. JHA26


enzyme_display_name: ginsenosidase type III

organism: Terrabacter ginsenosidimutans

substrate_name: ginsenoside Rb1

enzyme: ginsenosidase type III

substrate: ginsenoside Rb1


enzyme_display_name: ginsenoside Rb1 beta-glucosidase

organism: Pseudonocardia sp.

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

assay_conditions_summary: pH 7

enzyme: ginsenoside Rb1 beta-glucosidase

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Pseudothermotoga thermarum


organism: Thermotoga petrophila

assay_conditions_summary: pH 5


enzyme_display_name: prolyl oligopeptidase

organism: Bos taurus

substrate_name: H2O; Z-Gly-Pro-AMC

enzyme: prolyl oligopeptidase

substrate: H2O; Z-Gly-Pro-AMC


organism: Homo sapiens


enzyme_display_name: ureidoacrylate amidohydrolase

organism: Escherichia coli

enzyme: ureidoacrylate amidohydrolase


organism: Mycoplasma arginini


organism: Mycoplasma arthritidis


ki_unit: mM


organism: Thermococcus litoralis


enzyme_display_name: 4-hydroxyproline epimerase

organism: Agrobacterium tumefaciens

enzyme: 4-hydroxyproline epimerase


organism: Agrobacterium vitis


organism: Bacillus cereus


organism: Bacillus thuringiensis


organism: Brucella abortus


organism: Brucella melitensis


organism: Brucella melitensis biotype 1


organism: Pyrococcus horikoshii


organism: Ochrobactrum anthropi


organism: Paracoccus denitrificans


organism: Pseudomonas putida


organism: Rhizobium etli


organism: Rhizobium meliloti


organism: Shewanella loihica


organism: Streptosporangium roseum


organism: Brucella suis


organism: Burkholderia multivorans


organism: Burkholderia pseudomallei


organism: Burkholderia thailandensis


organism: Chromobacterium violaceum


organism: Chromohalobacter salexigens


organism: Ferroplasma acidarmanus


organism: Labrenzia aggregata


organism: Labrenzia alexandrii


organism: Xanthomonas campestris


enzyme_display_name: L-arabinose isomerase

organism: Acidothermus cellulolyticus

substrate_name: L-arabinose

enzyme: L-arabinose isomerase

substrate: L-arabinose


organism: Alicyclobacillus acidocaldarius

proof_lines:
wild-type at pH 6 and 65°C
wild-type at pH 7 and 65°C
wild-type at pH 8 and 65°C


substrate_name: L-Arabinose

substrate: L-Arabinose


proof_lines:
mutant K269E at pH 7 and 65°C
mutant K269E at pH 8 and 65°C
mutant K269E at pH 6 and 65°C


source_license: CC BY 4.0; CC BY-NC-ND 4.0

proof_lines:
pH 6.0, 65Â°C, recombinant enzyme
pH 7.5, 75Â°C, recombinant enzyme
Raw SKiD Env: Temp=65°C, pH=6

source_databases_merged: brenda skid


organism: Alicyclobacillus hesperidum

substrate_name: D-galactose

substrate: D-galactose


organism: Anoxybacillus flavithermus


assay_conditions_summary: pH 9.5; 368.15 K; buffer 50 mM glycine-NaOH

proof_lines: without borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C

proof_lines: with 10 mM borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C


organism: Arthrobacter sp.


organism: Bacillus halodurans


organism: Bacillus licheniformis


proof_lines: pH 7.5, 50°C, recombinant wild-type enzyme


proof_lines: pH and temperature not specified in the publication


organism: Bacillus subtilis

substrate_name: L-ribulose

proof_lines: pH 7.5, 23°C, recombinant His6-tagged wild-type enzyme

substrate: L-ribulose


organism: Bacillus velezensis


organism: Bifidobacterium adolescentis


organism: Geobacillus stearothermophilus

substrate_name: L-Fucose

substrate: L-Fucose


proof_lines: without borate, pH 8.0, 60°C, recombinant wild-type enzyme

proof_lines: with borate, pH 8.0, 60°C, recombinant wild-type enzyme


proof_lines: without borate, pH 8.0, 60°C, recombinant mutant H18T

proof_lines: with borate, pH 8.0, 60°C, recombinant mutant H18T


organism: Geobacillus thermodenitrificans

proof_lines: in the presence of 10 mM Mn2+, enzyme treated with EDTA

proof_lines: in the presence of 1 mM Mn2+, enzyme treated with EDTA


organism: Bifidobacterium longum

proof_lines:
at pH 6.5 and 45°C
pH 6.0-6.5, 55Â°C, recombinant enzyme


organism: [Clostridium] hylemonae


organism: Enterococcus faecium

proof_lines: N-His-tagged recombinant protein, Michaelis-Menten kinetics, pH 5.6, 50°C

proof_lines: C-His-tagged recombinant protein, K0.5 value, cooperative interaction model, pH 5.6, 50°C

proof_lines: pH 7.0-7.5, 45-50Â°C, recombinant enzyme


organism: Geobacillus kaustophilus

proof_lines:
pH 7.5, 22°C
Raw SKiD Env: Temp=22°C, pH=7.5


proof_lines: pH 8.0, 60°C, recombinant enzyme expressed in Escherichia coli

proof_lines: pH 8.0, 60°C, recombinant enzyme expressed in Bacillus subtilis


proof_lines:
mutant C450S/N475K, pH 8, 55°C
C450S /N475K mutant


organism: Halalkalibacterium halodurans


organism: Heyndrickxia coagulans


organism: Limosilactobacillus fermentum

proof_lines:
mutant enzyme D299R, at pH 4.0 and 65°C
mutant enzyme D299R, at pH 6.0 and 65°C
mutant enzyme D299R, at pH 5.0 and 65°C


proof_lines:
wild type enzyme, at pH 6.0 and 65°C
wild type enzyme, at pH 5.0 and 65°C


proof_lines:
mutant enzyme D269K, at pH 5.0 and 65°C
mutant enzyme D269K, at pH 4.0 and 65°C
mutant enzyme D269K, at pH 6.0 and 65°C


proof_lines: mutant enzyme D269K/D299K, at pH 5.0 and 65°C

proof_lines: mutant enzyme D269K/D299K, at pH 6.0 and 65°C

proof_lines: mutant enzyme D269K/D299K, at pH 4.0 and 65°C


proof_lines: mutant enzyme D268K/D269K/D299K, at pH 6.0 and 65°C

proof_lines: mutant enzyme D268K/D269K/D299K, at pH 4.0 and 65°C

proof_lines: mutant enzyme D268K/D269K/D299K, at pH 5.0 and 65°C


proof_lines:
mutant enzyme D299K, at pH 5.0 and 65°C
mutant enzyme D299K, at pH 6.0 and 65°C
mutant enzyme D299K, at pH 4.0 and 65°C


proof_lines:
mutant enzyme D268K, at pH 5.0 and 65°C
mutant enzyme D268K, at pH 6.0 and 65°C
mutant enzyme D268K, at pH 4.0 and 65°C


proof_lines: mutant enzyme D268K/D299K, at pH 6.0 and 65°C

proof_lines: mutant enzyme D268K/D299K, at pH 5.0 and 65°C

proof_lines: mutant enzyme D268K/D299K, at pH 4.0 and 65°C


assay_conditions_summary: pH 5; 338.15 K; buffer context

proof_lines: in 50 mM phosphate buffer (pH 6.5), 1mM Mn2+, 2mM Co2+, at 65°C


organism: Limosilactobacillus reuteri subsp. rodentium


organism: Parageobacillus thermoglucosidasius

proof_lines:
at pH 7.0 and 40°C
pH 7.0, 40Â°C, recombinant enzyme


organism: Pediococcus pentosaceus


organism: Lactiplantibacillus plantarum

proof_lines:
at 60°C, pH 7.5
pH 7.5, 60Â°C, recombinant enzyme


organism: Lactobacillus plantarum

substrate_name: D-Galactose

substrate: D-Galactose


organism: Lactobacillus sakei


organism: Latilactobacillus sakei subsp. sakei


organism: Lentilactobacillus parabuchneri


organism: Levilactobacillus brevis


proof_lines: mutant enzyme D268K/D269K, at pH 6.0 and 65°C

proof_lines: mutant enzyme D268K/D269K, at pH 4.0 and 65°C

proof_lines: mutant enzyme D268K/D269K, at pH 5.0 and 65°C


enzyme_display_name: 2-hydroxymuconate tautomerase

organism: Archaeoglobus fulgidus

proof_lines: pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate

enzyme: 2-hydroxymuconate tautomerase


organism: Bacillus subtilis


organism: Burkholderia lata


organism: Chloroflexus aurantiacus

substrate_name: phenylenolpyruvate

proof_lines: pH 7.3, 24°C, recombinant wild-type hh4-OT

substrate: phenylenolpyruvate


proof_lines: pH 7.3, 24°C, recombinant hh4-OT mutant betaR11A

proof_lines: pH 7.3, 24°C, recombinant hh4-OT mutant betaP1A

proof_lines: pH 7.3, 24°C, recombinant hh4-OT mutant alphaR40A


organism: Gammaproteobacteria bacterium


organism: Leptothrix cholodnii


assay_conditions_summary: pH 6


organism: Pseudoalteromonas haloplanktis


organism: Shewanella sp. ANA-3


proof_lines: pH 5.5-6.5, 15-35Â°C, recombinant enzyme


organism: Thermoanaerobacter mathranii

substrate_name: D-fucose

substrate: D-fucose


organism: Thermoanaerobacter mathranii subsp. mathranii

assay_conditions_summary: pH 8


organism: Thermoanaerobacterium saccharolyticum

assay_conditions_summary: pH 7.5; 333.15 K; buffer 25 mM Tris-HCl

proof_lines: in 25 mM Tris-HCl buffer (pH 7.5), at 60°C


organism: Thermotoga maritima


organism: Thermotoga neapolitana

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)


organism: Methylibium petroleiphilum

assay_conditions_summary: pH 7.3; 297.15 K; buffer 10 mM potassium phosphate

proof_lines: isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C

proof_lines: isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C


organism: Pseudomonas putida

substrate_name: (2E)-2-Hydroxypenta-2,4-dienoic acid

substrate: (2E)-2-Hydroxypenta-2,4-dienoic acid


enzyme_display_name: isopentenyl-diphosphate Delta-isomerase

organism: Escherichia coli

substrate_name: isopentenyl diphosphate

enzyme: isopentenyl-diphosphate Delta-isomerase

substrate: isopentenyl diphosphate


proof_lines:
wild-type enzyme
wild-type, pH 7.4, 37°C


substrate_name: Isopentenyl diphosphate

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

proof_lines: pH and temperature not specified in the publication

source_databases_merged: sabio_rk brenda skid

substrate: Isopentenyl diphosphate


organism: Homo sapiens


organism: Saccharolobus shibatae

smiles: C=C(C)CCOP(=O)(O)OP(=O)(O)O


assay_conditions_summary: pH 6; 333.15 K; cofactor NADH

proof_lines: dehydrogenase reaction without isopentenyl diphosphate


organism: Streptomyces sp.


organism: Sulfolobus shibatae


organism: Saccharomyces cerevisiae


proof_lines: mutant enzyme L141H/Y195F/W256C, at pH 7.5 and 30°C


organism: Staphylococcus aureus


organism: Streptococcus pneumoniae


organism: Nepenthes x ventrata

substrate_name: fluorogenic FS6 peptide

source_db: uniprot

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: kcat is 1.81 sec(-1) for the cleavage of the fluorogenic FS6 peptide (PubMed:35915115). kcat is 6.55 sec(-1) for the cleavage of the fluorogenic FS6-QPQL peptide (PubMed:35915115).

substrate: fluorogenic FS6 peptide

source: uniprot


enzyme_display_name: acetylsalicylate deacetylase

organism: Rattus norvegicus

substrate_name: 2-Nitrophenyl butyrate

proof_lines:
pH 7.8, 25°C, isozyme I
pH 7.8, 25°C, isozyme II
pH 7.4, 37°C, isozyme I

enzyme: acetylsalicylate deacetylase

substrate: 2-Nitrophenyl butyrate


organism: Unknown


substrate_name: UDP-glucuronate

substrate: UDP-glucuronate


proof_lines:
37°C, pH 6.5, wild-type enzyme
wild-type enzyme


organism: Geobacillus stearothermophilus


proof_lines: mutant enzyme Q160L, in 50 mM MOPS-NaOH, pH 6.0, at 60°C


proof_lines: mutant enzyme Q160K, in 50 mM MOPS-NaOH, pH 6.0, at 60°C


proof_lines: wild type enzyme, in 50 mM MOPS-NaOH, pH 6.0, at 60°C


proof_lines: mutant enzyme Q160E, in 50 mM MOPS-NaOH, pH 6.0, at 60°C


proof_lines: mutant enzyme Q160N, in 50 mM MOPS-NaOH, pH 6.0, at 60°C


proof_lines: mutant enzyme Q160H, in 50 mM MOPS-NaOH, pH 6.0, at 60°C


assay_conditions_summary: pH 7.3; 296.15 K; buffer 10 mM potassium phosphate

proof_lines: mutant enzyme R39A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C


proof_lines: mutant enzyme R11A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C


proof_lines:
below, pH 7.3, 23°C, mutant R11A/R39A
pH 7.3, 23°C, mutant R11A/R39A


proof_lines: wild type enzyme, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C


organism: Choristoneura fumiferana

proof_lines: mutant enzyme Y159F, in 25 mM Tris-HCl, pH 7.0, at 37°C


proof_lines: mutant enzyme Y105F, in 25 mM Tris-HCl, pH 7.0, at 37°C


proof_lines: mutant enzyme E169Q, in 25 mM Tris-HCl, pH 7.0, at 37°C


proof_lines: mutant enzyme W216F, in 25 mM Tris-HCl, pH 7.0, at 37°C


enzyme_display_name: homoglutathione synthase

organism: Glycine max

substrate_name: beta-Alanine

enzyme: homoglutathione synthase

substrate: beta-Alanine


substrate_name: ATP

assay_conditions_summary: cofactor ATP

substrate: ATP


substrate_name: glycine

substrate: glycine


proof_lines: wild type enzyme, in 25 mM Tris-HCl, pH 7.0, at 37°C


organism: Clavibacter michiganensis

substrate_name: gypenoside XVII

substrate: gypenoside XVII


proof_lines: pH 7.5, 35°C, recombinant wild-type enzyme


substrate_name: ginsenoside Rb1

substrate: ginsenoside Rb1


enzyme_display_name: thymidine-triphosphatase

organism: Escherichia phage T7

substrate_name: dTTP

proof_lines: mutant enzyme R493A/N468A, in the presence of M13 single-stranded DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C

proof_lines: mutant enzyme R493A/N468A, in the absence of DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C

enzyme: thymidine-triphosphatase

substrate: dTTP


proof_lines: mutant enzyme N468A, in the absence of DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C

proof_lines: mutant enzyme N468A, in the presence of M13 single-stranded DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C


proof_lines: mutant enzyme R493Q, in the presence of M13 single-stranded DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C

proof_lines: mutant enzyme R493Q, in the absence of DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C


proof_lines: wild type enzyme, in the presence of M13 single-stranded DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C

proof_lines: wild type enzyme, in the absence of DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C


organism: Pseudomonas putida


substrate_name: D-galactose

proof_lines:
recombinant mutant H18T, pH 6.0, 60°C
recombinant mutant H18T, pH 7.5, 60°C

substrate: D-galactose


proof_lines: recombinant mutant H18T/Y234C, pH 6.0, 60°C

proof_lines: recombinant mutant H18T/Y234C, pH 7.5, 60°C


organism: Halalkalibacterium halodurans

substrate_name: L-arabinose

proof_lines:
wild-type at pH 6 and 50°C
wild-type at pH 7 and 50°C
wild-type at pH 8 and 50°C

substrate: L-arabinose


proof_lines:
mutant E268K at pH 6 and 50°C
mutant E268K at pH 7 and 50°C
mutant E268K at pH 8 and 50°C


assay_conditions_summary: pH 7.3; 295.15 K; buffer 10 mM potassium phosphate

proof_lines: mutant enzyme P1A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C


proof_lines: mutant enzyme R61A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C


proof_lines: wild type enzyme, at aerobic conditions, at pH 7.0 and 37°C

proof_lines: wild type enzyme, at anaerobic conditions, at pH 7.0 and 37°C


proof_lines: mutant enzyme N37A, at aerobic conditions, at pH 7.0 and 37°C


enzyme_display_name: iodide peroxidase

organism: Ascophyllum nodosum

substrate_name: I-

assay_conditions_summary: pH 8; 293.15 K; buffer phosphate

proof_lines: in phosphate buffer (100 mM, pH 8.0), at 20°C

enzyme: iodide peroxidase

substrate: I-


organism: Homo sapiens

substrate_name: benzidine

substrate: benzidine


organism: Sus scrofa


assay_conditions_summary: pH 7; 298.15 K; cofactor FAD

proof_lines:
FAD cofactor, pH 7, 25°C
native enzyme, pH 7, 25°C
2’-deoxy-FAD cofactor, pH 7, 25°C


enzyme_display_name: protein-disulfide reductase

organism: Lathyrus oleraceus

substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

enzyme: protein-disulfide reductase

substrate: NADPH


organism: Mycobacterium tuberculosis

substrate_name: NADH

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

proof_lines: pH 7.5, 25°C, with 2,6-dimethyl-1,4-benzoquinone

proof_lines: pH 7.5, 25°C, with 5-hydroxy-1,4-naphthoquinone

substrate: NADH


organism: Priestia megaterium

substrate_name: pantethine 4',4''-diphosphate

assay_conditions_summary: cofactor CoA

substrate: pantethine 4',4''-diphosphate


organism: Escherichia coli

substrate_name: methyl deficient tRNA from E. coli

substrate: methyl deficient tRNA from E. coli


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: undermethylated tRNA

substrate: undermethylated tRNA


organism: denitrifying bacterium

substrate_name: 4-Hydroxybenzyl alcohol

substrate: 4-Hydroxybenzyl alcohol


organism: Geobacter metallireducens

proof_lines:
competitive inhibition
uncompetitive inhibition


substrate_name: UDPglucuronate

substrate: UDPglucuronate


substrate_name: UDP-glucuronate

substrate: UDP-glucuronate


organism: Mus musculus


enzyme_display_name: mogroside IE synthase

organism: Siraitia grosvenorii

substrate_name: quercetin

enzyme: mogroside IE synthase

substrate: quercetin


enzyme_display_name: aminolevulinate transaminase

organism: Bos taurus

proof_lines: pH 7.0, 37°C, 4,5-dioxopentanoate as amino acceptor

proof_lines: pH 7.0, 37°C, glyoxylate as amino acceptor

enzyme: aminolevulinate transaminase


organism: Candida albicans

substrate_name: L-alanine

substrate: L-alanine


organism: Cenchrus americanus


organism: Cereibacter sphaeroides


organism: Leishmania donovani


organism: Raphanus sativus


organism: Rattus norvegicus

proof_lines: different concentrations of alanine at fixed 4,5-dioxopentanoate concentration


organism: Chlorella regularis

substrate_name: glyoxylate

proof_lines: pH 8.0, 37°C, glyoxylate as amino acceptor

substrate: glyoxylate


organism: Clostridium tetanomorphum


organism: Devosia riboflavina


organism: Gallus gallus

assay_conditions_summary: pH 7


organism: Brevibacterium sp.

substrate_name: ADP

assay_conditions_summary: pH 8.4; cofactor ADP

substrate: ADP


organism: Cucumis sativus

substrate_name: UDP

substrate: UDP


organism: Escherichia coli

proof_lines: pH 8.0, 37°C, phosphorolysis of poly(A)tail from rabbit globin mRNA


organism: Kitasatospora aureofaciens

assay_conditions_summary: pH 9.5; 310.15 K; cofactor ADP

proof_lines: approx. value, also for CDP and UDP polymerization


substrate_name: 8-mer 2' phosphorylated RNA

proof_lines:
37°C
pUpU-(2’-phosphate)pU, 37°C
pApA-(2’-phosphate)pA, 37°C

substrate: 8-mer 2' phosphorylated RNA


organism: Saccharomyces cerevisiae

substrate_name: 3-mer 2' phosphorylated RNA

substrate: 3-mer 2' phosphorylated RNA


organism: Micrococcus luteus

substrate_name: heptauridylate

assay_conditions_summary: pH 9; 310.15 K; cofactor ADP

proof_lines: pH 9.0, 37°C, inhibition of ADP polymerization

substrate: heptauridylate


organism: Rhodospirillum rubrum

substrate_name: phosphate

assay_conditions_summary: pH 8; 293.15 K; cofactor ADP

proof_lines: pH 8.0, 20°C, ADP-phosphate-exchange reaction

proof_lines: pH 8.0, 20°C, ADP-phosphate exchange reaction

substrate: phosphate


organism: Sinorhizobium meliloti

substrate_name: Poly(A)

substrate: Poly(A)


organism: Thermus thermophilus

substrate_name: Polyadenylic acid

proof_lines: synthesis of a copolymer of adenylic acid

proof_lines: synthesis of a copolymer of uridylic acid

proof_lines: synthesis of a copolymer of guanylic acid

substrate: Polyadenylic acid


enzyme_display_name: chondroitin 6-sulfotransferase

organism: Mus musculus

substrate_name: native chondroitin

enzyme: chondroitin 6-sulfotransferase

substrate: native chondroitin


organism: Cavia porcellus

substrate_name: n-Decanoyl salicylate

substrate: n-Decanoyl salicylate


organism: Homo sapiens

substrate_name: Acetylsalicylic acid

proof_lines: recombinant catalytic subuit PAFAH1B2, pH 7.2, 37°C

substrate: Acetylsalicylic acid


organism: Bos taurus

substrate_name: decasaccharide

substrate: decasaccharide


organism: Coturnix japonica

substrate_name: chondroitin

proof_lines: 1-phospho-N-acetylgalactosamine 4-sulfate, pH 7, 37°C

proof_lines: + UDP-N-acetylgalactosamine 4-sulfate, pH 7, 37°C

substrate: chondroitin


proof_lines: in the presence of protamine, pH 8, 37°C


substrate_name: chondroitin 4-sulfate

substrate: chondroitin 4-sulfate


enzyme_display_name: poly(ADP-ribose) glycohydrolase

substrate_name: Poly(ADP-ribose)

assay_conditions_summary: 310.15 K; cofactor ADP

enzyme: poly(ADP-ribose) glycohydrolase

substrate: Poly(ADP-ribose)


substrate_name: (ADP-ribose)n

assay_conditions_summary: cofactor ADP

proof_lines: average chain length n = 21, poly(ADP-ribose) glycohydrolase II

proof_lines: average chain length n = 15, poly(ADP-ribose) glycohydrolase I

substrate: (ADP-ribose)n


organism: Sus scrofa


enzyme_display_name: beta-galactofuranosidase

organism: Aspergillus niger

substrate_name: p-nitrophenyl beta-D-galactofuranoside

enzyme: beta-galactofuranosidase

substrate: p-nitrophenyl beta-D-galactofuranoside


organism: Penicillium fellutanum

substrate_name: peptidophosphogalactomannan pP2GM

assay_conditions_summary: pH 4; 313.15 K; buffer 66 mM NaOAc

proof_lines:
40°C, pH 4
in 66 mM NaOAc buffer (pH 4.9), at 37°C
pH 4.6, 37°C

substrate: peptidophosphogalactomannan pP2GM


organism: Streptomyces sp. JHA 19

substrate_name: 4-nitrophenyl beta-D-galactofuranoside

substrate: 4-nitrophenyl beta-D-galactofuranoside


enzyme_display_name: ginsenosidase type III

organism: Panax notoginseng

substrate_name: ginsenoside Rb3

enzyme: ginsenosidase type III

substrate: ginsenoside Rb3


enzyme_display_name: ginsenoside Rb1 beta-glucosidase

organism: Gordonia terrae

substrate_name: ginsenoside Rd

proof_lines:
recombinant enzyme, pH 6.5, 30°C
at pH 6.5 and 30Â°C

enzyme: ginsenoside Rb1 beta-glucosidase

substrate: ginsenoside Rd


organism: Mucilaginibacter sp.

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Paecilomyces sp.

substrate_name: 4-nitrophenyl beta-D-glucoside

substrate: 4-nitrophenyl beta-D-glucoside


organism: Thermoclostridium stercorarium


enzyme_display_name: leucyl endopeptidase

organism: Bacillus cereus

enzyme: leucyl endopeptidase


organism: Spinacia oleracea


organism: Thermotoga petrophila

proof_lines:
isoform bgl1, at pH 6.0 and 90°C
isoform blg1, at pH 6.0 and 90°C


enzyme_display_name: kexin

organism: Mus musculus

substrate_name: Unknown

enzyme: kexin

substrate: Unknown


organism: Saccharomyces cerevisiae

proof_lines:
pH 7.0, 21°C
pH 7.0, 37°C
pH 7.0, 37°C, in natural abundance H2O


enzyme_display_name: arginine deiminase

organism: Pseudomonas plecoglossicida

substrate_name: L-arginine

assay_conditions_summary: pH 7.4; 310.15 K; buffer 0.5 M phosphate

proof_lines: mutant enzyme H404R, in 0.5 M phosphate buffer, pH 7.4, at 37°C

enzyme: arginine deiminase

substrate: L-arginine


enzyme_display_name: proprotein convertase 2

enzyme: proprotein convertase 2


organism: Bacillus subtilis

substrate_name: dTTP

substrate: dTTP


organism: Homo sapiens

proof_lines: competitive inhibitor with dTTP as substrate


enzyme_display_name: 4-hydroxyproline epimerase

organism: Pseudomonas aeruginosa

proof_lines: in 50 mM HEPES, pH 8.0, with 2 mM beta-mercaptoethanol,1 mM EDTA, at 37°C

enzyme: 4-hydroxyproline epimerase


organism: Pseudomonas putida

proof_lines:
solvent D2O
solvent H2O
2-2H-4-hydroxy-L-Pro


organism: Sinorhizobium meliloti

proof_lines: pH not specified in the publication, 30°C, recombinant enzyme


enzyme_display_name: L-arabinose isomerase

organism: Halalkalibacterium halodurans

substrate_name: D-galactose

enzyme: L-arabinose isomerase

substrate: D-galactose


organism: Heyndrickxia coagulans

substrate_name: L-arabinose

substrate: L-arabinose


organism: Lactiplantibacillus plantarum

substrate_name: L-ribulose

substrate: L-ribulose


organism: Lactobacillus gayonii


organism: Bacillus licheniformis

assay_conditions_summary: pH 7.5; 323.15 K; buffer 50 mM phosphate

proof_lines: pH 7.5, 50°C, immobilized recombinant enzyme

proof_lines: immobilized enzyme, in 50 mM phosphate buffer (pH 7.5),1 mM MnCl2, at 50°C


organism: Enterococcus faecium

proof_lines:
at pH 7.0 and 40°C
at pH 7.0 and 30°C
at pH 7.0 and 50°C


organism: Escherichia coli

substrate_name: D-tagatose

proof_lines:
pH 7.0, 37°C, whole cells
pH 7.0, 37°C, purified enzyme

substrate: D-tagatose


organism: Geobacillus stearothermophilus

proof_lines: in the presence of 0.4 mM Mn2+, at pH 7.5 and 60°C

proof_lines: in the presence of 0.2 mM Mn2+, at pH 7.5 and 60°C


organism: Latilactobacillus sakei

assay_conditions_summary: pH 5; buffer 100 mM sodium acetate

proof_lines: with 0.8 mM MgCl2 and 0.8 mM MnCl2, in 100 mM sodium acetate buffer (pH 5.0), at 35°C


organism: Mycolicibacterium smegmatis

proof_lines:
D-galactose induced enzyme
L-arabinose induced enzyme


organism: Pseudoalteromonas haloplanktis

proof_lines:
pH 8.0, 40Â°C, recombinant enzyme
pH 7.5, 75Â°C, recombinant enzyme


organism: Streptomyces sp.


organism: Thermotoga maritima

proof_lines: in the presence of 0.6 mM Mn2+, at pH 7.5 and 60°C

proof_lines: in the presence of 0.8 mM Mn2+, at pH 7.5 and 60°C


organism: Thermotoga neapolitana


enzyme_display_name: 2-hydroxymuconate tautomerase

organism: Helicobacter pylori

substrate_name: phenylenolpyruvate

proof_lines: pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate

enzyme: 2-hydroxymuconate tautomerase

substrate: phenylenolpyruvate


organism: Pseudomonas putida

proof_lines:
pH 7.3, 30°C
at pH 7.3 and 22°C
L-4OT enantiomer, pH 7.3, 30°C


enzyme_display_name: isopentenyl-diphosphate Delta-isomerase

organism: Aedes aegypti

substrate_name: isopentenyl diphosphate

proof_lines: in 0.4 M Tris, 1 mM dithiothreitol, 10 mM MgCl2, pH 8.0, at 50°C

enzyme: isopentenyl-diphosphate Delta-isomerase

substrate: isopentenyl diphosphate


organism: Bombyx mori


organism: Capsicum annuum


organism: Claviceps sp.


organism: Methanothermobacter thermautotrophicus


organism: Narcissus pseudonarcissus


organism: Saccharolobus shibatae

assay_conditions_summary: pH 6


organism: Saccharomyces cerevisiae


organism: Escherichia coli

proof_lines: in 50 mM HEPES (pH 7.2) containing 10 mM MgCl2, 200 mM KCl, 1 mg/ml bovine serum albumin, 0.5 mM dithiothreitol, at 37°C


organism: Gallus gallus

proof_lines:
isomerase IV
isomerase I
isomerase II and III


organism: Homo sapiens

assay_conditions_summary: pH 8


organism: Methanocaldococcus jannaschii


organism: Solanum lycopersicum


organism: Staphylococcus aureus

assay_conditions_summary: pH 7; 310.15 K; cofactor NADPH

proof_lines: aerobic, presence of dithionite, pH 7.0, 37°C

proof_lines: anaerobic, presence of NADPH, pH 7.0, 37°C


organism: Sus scrofa


organism: Thermococcus kodakarensis


organism: Thermus thermophilus

proof_lines: in 200 mM HEPES (pH 7.0) containing 2 mM MgCl2, 0.04 mM FMN, 2 mM NADPH, at 37°C


enzyme_display_name: tryptase

proof_lines:
pH 7.6, 20°C, isoform L1
pH 6.0, 25°C, 200 ng protamine
pH 7.6, 20°C, isoform L2

enzyme: tryptase


enzyme_display_name: homoglutathione synthase

organism: Lathyrus oleraceus

substrate_name: beta-Alanine

enzyme: homoglutathione synthase

substrate: beta-Alanine


organism: Phaseolus coccineus

substrate_name: gamma-glutamylcysteine

substrate: gamma-glutamylcysteine


organism: Vigna radiata var. radiata

substrate_name: ATP

assay_conditions_summary: cofactor ATP

substrate: ATP


organism: Trichomonas vaginalis

substrate_name: H2O; L-Arginine

proof_lines:
strain MR-5
strain MR-100
at pH 6.0 and 37°C

substrate: H2O; L-Arginine


organism: Cinchona robusta

substrate_name: Isopentenyl diphosphate

proof_lines:
isomerase II
isomerase I

substrate: Isopentenyl diphosphate


organism: Mycoplasma arthritidis


enzyme_display_name: aspartate racemase

organism: Enterococcus hirae

substrate_name: L-Aspartate

enzyme: aspartate racemase

substrate: L-Aspartate


enzyme_display_name: cellulase

organism: Pyrococcus horikoshii

substrate_name: p-nitrophenyl cellobiose

enzyme: cellulase

substrate: p-nitrophenyl cellobiose


proof_lines: pH 5-6, 50°C, mutant enzyme lacking 5 residues at the C-terminus and 5 residies at the N-terminus


substrate_name: 4-nitrophenyl cellobioside

proof_lines:
pH 6.0, 70°C
pH 5.5, 50°C
Raw SKiD Env: Temp=50°C, pH=5.5

substrate: 4-nitrophenyl cellobioside


organism: Rasamsonia emersonii

substrate_name: 4-methylumbelliferyl beta-D-lactoside

substrate: 4-methylumbelliferyl beta-D-lactoside


organism: Pseudomonas syringae

smiles: O=C(O)CCC(=O)C(=O)O


enzyme_display_name: steroid C-25 hydroxylase

organism: Sterolibacterium denitrificans

proof_lines: pH 7.5, 30°C, purified enzyme, immobilized enzyme

enzyme: steroid C-25 hydroxylase


organism: Nicotiana attenuata

substrate_name: caffeoyl-CoA

assay_conditions_summary: pH 7.5; 303.15 K; cofactor CoA

proof_lines: with putrescine, recombinant His-tagged enzyme, pH 7.5, 30°C

proof_lines: with caffeoyl-CoA, recombinant His-tagged enzyme, pH 7.5, 30°C

substrate: caffeoyl-CoA


organism: Oryza sativa Japonica Group

substrate_name: feruloyl-CoA

proof_lines: cosubstrate 4-coumaroyl-CoA, pH 7.5, 30°C

substrate: feruloyl-CoA


organism: Solanum lycopersicum

assay_conditions_summary: pH 9; 308.15 K; cofactor CoA


substrate_name: putrescine

substrate: putrescine


organism: Bacillus subtilis

substrate_name: H2O; 4-Nitrophenyl-beta-cellobioside

substrate: H2O; 4-Nitrophenyl-beta-cellobioside


organism: Cellulomonas fimi


organism: Pyrococcus furiosus


substrate_name: 4-nitrophenyl cellotetraoside

substrate: 4-nitrophenyl cellotetraoside


substrate_name: 4-nitrophenyl cellotrioside

substrate: 4-nitrophenyl cellotrioside


substrate_name: 4-nitrophenyl cellopentaoside

substrate: 4-nitrophenyl cellopentaoside


proof_lines: 50°C, mutant enzyme C106A/C159A/C372A/C412A


organism: Thermobifida fusca

substrate_name: 2,4-dinitrophenyl beta-D-cellobioside

substrate: 2,4-dinitrophenyl beta-D-cellobioside


organism: Thermotoga maritima

proof_lines: pH 3, 80°C, chimeric fusion protein CelA-SSO1949-CelA, that consists of 70 amino acids CelA followed by 163 amino acids SSO1949 and 29 amino acids CelA


organism: Trichoderma reesei

substrate_name: 4-methylumbelliferyl cellobioside

substrate: 4-methylumbelliferyl cellobioside


substrate_name: 4-nitrophenyl D-cellobioside

substrate: 4-nitrophenyl D-cellobioside


organism: Homo sapiens

substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


organism: Bacillus sp.

substrate_name: H2O; Carboxymethylcellulose

substrate: H2O; Carboxymethylcellulose


substrate_name: p-Nitrophenyl-beta-D-cellotrioside

substrate: p-Nitrophenyl-beta-D-cellotrioside


substrate_name: p-Nitrophenyl-beta-D-cellotrioside; H2O

substrate: p-Nitrophenyl-beta-D-cellotrioside; H2O


enzyme_display_name: leukotriene-C4 synthase

substrate_name: leukotriene A4

proof_lines:
human recombinant enzyme
IC50 value

enzyme: leukotriene-C4 synthase

substrate: leukotriene A4


proof_lines: mutant R31A, 22°C, pH not specified in the publication


proof_lines: mutant R31Q, 22°C, pH not specified in the publication


proof_lines: mutant R104S, 22°C, pH not specified in the publication


substrate_name: glutathione

substrate: glutathione


substrate_name: leukotriene A4 methyl ester

substrate: leukotriene A4 methyl ester


proof_lines: mutant R104A, 22°C, pH not specified in the publication


proof_lines: wild-type, 22°C, pH not specified in the publication


proof_lines: pH 7.8, temperature not specified in the publication, recombinant mutant W116A


organism: uncultured bacterium

substrate_name: cellotriose

substrate: cellotriose


substrate_name: carboxymethyl cellulose

substrate: carboxymethyl cellulose


substrate_name: cellopentaose

substrate: cellopentaose


substrate_name: Laminarin

substrate: Laminarin


substrate_name: cellohexaose

substrate: cellohexaose


substrate_name: hydroxyethylcellulose

substrate: hydroxyethylcellulose


substrate_name: cellotetraose

substrate: cellotetraose


enzyme_display_name: pepsin B

organism: Canis lupus familiaris

substrate_name: KPAEFFRL

enzyme: pepsin B

substrate: KPAEFFRL


substrate_name: KPAGFARL

substrate: KPAGFARL


substrate_name: KPAEFARL

substrate: KPAEFARL


organism: Pyrococcus horikoshii

substrate_name: L-Aspartate

substrate: L-Aspartate


organism: Thermococcus litoralis


organism: Escherichia coli

substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


organism: Methanococcus maripaludis


organism: Neisseria meningitidis serogroup A / serotype 4A

substrate_name: UDP-N-acetyl-alpha-D-glucosamine

substrate: UDP-N-acetyl-alpha-D-glucosamine


substrate_name: UDP-N-acetyl-alpha-D-mannosamine

proof_lines:
pH 8.5, 37°C, recombinant enzyme
Raw SKiD Env: Temp=37°C, pH=8.5

substrate: UDP-N-acetyl-alpha-D-mannosamine


organism: Rattus norvegicus

substrate_name: UDP-N-acetyl-D-glucosamine; H2O

substrate: UDP-N-acetyl-D-glucosamine; H2O


substrate_name: leukotriene A4

proof_lines: pH 7.8, temperature not specified in the publication, recombinant mutant W116F

substrate: leukotriene A4


substrate_name: glutathione

substrate: glutathione


substrate_name: Leukotriene A4; Reduced glutathione

substrate: Leukotriene A4; Reduced glutathione


substrate_name: Reduced glutathione; Leukotriene A4

substrate: Reduced glutathione; Leukotriene A4


organism: Mus musculus

proof_lines: pH 7.8, temperature not specified in the publication, recombinant enzyme


substrate_name: leukotriene A4 methyl ester

substrate: leukotriene A4 methyl ester


organism: Acropora millepora


organism: Crassostrea gigas


organism: Penaeus monodon


enzyme_display_name: mannose-6-phosphate isomerase

organism: Thermoplasma acidophilum

substrate_name: D-Mannose 6-phosphate

enzyme: mannose-6-phosphate isomerase

substrate: D-Mannose 6-phosphate


organism: Thermus thermophilus

substrate_name: D-mannose 6-phosphate

substrate: D-mannose 6-phosphate


substrate_name: D-fructose 6-phosphate

substrate: D-fructose 6-phosphate


enzyme_display_name: dethiobiotin synthase

substrate_name: (7R,8S)-7,8-diaminopelargonic acid

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

enzyme: dethiobiotin synthase

substrate: (7R,8S)-7,8-diaminopelargonic acid


substrate_name: GTP

substrate: GTP


substrate_name: H2O; UDP-N-acetyl-D-mannosamine

substrate: H2O; UDP-N-acetyl-D-mannosamine


organism: Aeropyrum pernix


organism: Arabidopsis thaliana


organism: Bacillus subtilis

substrate_name: D-ribose

substrate: D-ribose


organism: Burkholderia cepacia

proof_lines: pH 7.6, 5 mM Mg2+, temperature not specified in the publication


organism: Candida albicans

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Chlorella variabilis


organism: Geobacillus thermodenitrificans

proof_lines: mutant enzyme W17Q/N90A/L129F, at pH 7.0 and 70°C


proof_lines: mutant enzyme N90A/L129F, at pH 7.0 and 70°C


organism: Lactobacillus casei


organism: Oryza sativa Japonica Group


organism: Pseudomonas aeruginosa


organism: Pyrobaculum aerophilum


organism: Pyrococcus horikoshii

substrate_name: beta-D-mannose 6-phosphate

substrate: beta-D-mannose 6-phosphate


organism: Rattus norvegicus


organism: Saccharomyces cerevisiae


organism: Sterolibacterium denitrificans

proof_lines:
isoenzyme S25DH4, pH 7.0, 30°C
isoenzyme S25DH3, pH 7.0, 30°C
isoenzyme S25DH2, pH 7.0, 30°C


organism: Escherichia coli

substrate_name: ATP; CO2; 7,8-Diaminononanoate

substrate: ATP; CO2; 7,8-Diaminononanoate


organism: Mycobacterium tuberculosis


substrate_name: CTP

substrate: CTP


substrate_name: TTP

substrate: TTP


organism: Thermus thermophilus

substrate_name: L-ribulose

substrate: L-ribulose


substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


substrate_name: vitamin D3

substrate: vitamin D3


substrate_name: mannose 6-phosphate

assay_conditions_summary: buffer selenomethionine-labelled enzyme Hepes buffer Tris/HCl buffer selenomethionine-labelled enzyme

substrate: mannose 6-phosphate


organism: Nicotiana tabacum

substrate_name: feruloyl-CoA

assay_conditions_summary: pH 10; cofactor CoA

proof_lines:
with caffeoyl-CoA
with 0.25 mM caffeoyl-CoA
pH 10.0

substrate: feruloyl-CoA


substrate_name: asialo-alpha1-acid glycoprotein

proof_lines:
PC-12D cells
PC-12 and PC-12D cells
PC-12 cells

substrate: asialo-alpha1-acid glycoprotein


substrate_name: 2-Aminoethylarsonic acid

substrate: 2-Aminoethylarsonic acid


organism: Bos taurus

substrate_name: lactose

substrate: lactose


organism: Helicobacter pylori

assay_conditions_summary: pH 7.5; 310.15 K; buffer cacodylate

proof_lines: using Galbeta(1->4)GlcNAcbeta-O(CH2)3N3 as acceptor, in cacodylate buffer (100 mM, pH 7.5), at 37°C

proof_lines: using Galbeta(1->3)GlcNAcbeta-O(CH2)3N3 as acceptor, in cacodylate buffer (100 mM, pH 7.5), at 37°C

proof_lines: using Galbeta(1->3)GalNAcalpha-OCH(CH3)CH(NH2)CO2H as acceptor, in cacodylate buffer (100 mM, pH 7.5), at 37°C


organism: Homo sapiens

proof_lines:
lactosylceramide as acceptor
lactose as acceptor
recombinant enzyme, pH 7.2, 37°C


organism: Mus musculus

substrate_name: neolactotetraosylceramide

proof_lines:
membrane-bound enzyme
solubilized enzyme

substrate: neolactotetraosylceramide


enzyme_display_name: oligonucleotidase

proof_lines:
in the presence of 3 mM Mn2+
in the presence of 1 mM Mn2+

enzyme: oligonucleotidase


organism: Flammulina velutipes


organism: Mycolicibacterium smegmatis


substrate_name: p-nitrophenyl uridine 5'-monophosphate

substrate: p-nitrophenyl uridine 5'-monophosphate


organism: Vibrio cholerae


enzyme_display_name: lactase

organism: Sus scrofa

enzyme: lactase


organism: Cavia porcellus

substrate_name: leukotriene A4 methyl ester

substrate: leukotriene A4 methyl ester


substrate_name: glutathione

substrate: glutathione


substrate_name: leukotriene A4

substrate: leukotriene A4


substrate_name: phlorizin

proof_lines:
LPH from uninfected cells
PPH from RRV-infected cells

substrate: phlorizin


organism: Ovis aries


organism: Platyrrhini


organism: Rattus norvegicus

assay_conditions_summary: pH 6; 298.15 K; buffer assay notes

proof_lines: 100 mM sodium maleate buffer, pH 6.0, 25°C

proof_lines: 100 mM sodium malaete buffer, pH 6.0, 25°C


organism: Albatrossia pectoralis

substrate_name: hemoglobin

substrate: hemoglobin


organism: Boreogadus saida


organism: Bacillus cereus

substrate_name: UDP-N-acetyl-alpha-D-mannosamine

proof_lines: in presence of 0.5 mM UDP-N-acetylglucosamine

substrate: UDP-N-acetyl-alpha-D-mannosamine


organism: Escherichia coli

proof_lines: in presence of 1 mM UDP-N-acetylglucosamine


proof_lines:
37°C, pH 7.5
tumor enzyme
and a second Km-value of 0.08 mM


organism: Cassia coluteoides

proof_lines:
enzyme from germinating seeds
enzyme from developing seeds


organism: Dehalococcoides mccartyi

substrate_name: D-mannose 6-phosphate

substrate: D-mannose 6-phosphate


substrate_name: beta-D-mannose 6-phosphate

assay_conditions_summary: pH 7.1; 298.15 K; buffer 50 mM HEPES

proof_lines:
in 50 mM HEPES buffer, pH 7.1, at 25°C
22°C, pH 7.4
pH 7.1, 25°C, recombinant enzyme

substrate: beta-D-mannose 6-phosphate


proof_lines:
in 50 mM HEPES buffer, pH 7.1, at 25°C
recombinant enzyme


organism: Amorphophallus konjac


organism: Arabidopsis thaliana


organism: Bacillus amyloliquefaciens


organism: Burkholderia cenocepacia


organism: Xanthomonas campestris


organism: Homo sapiens


organism: Pseudomonas aeruginosa

proof_lines: bifunctional enzyme: phosphomannose isomerase-guanosine 5-diphospho-D-mannose pyrophosphorylase


organism: Pyrobaculum aerophilum

substrate_name: D-fructose 6-phosphate

substrate: D-fructose 6-phosphate


organism: Saccharomyces cerevisiae

proof_lines:
pH 7.1, 25°C
pH 7.1, 25°C, recombinant enzyme


substrate_name: Reduced glutathione; Leukotriene A4

substrate: Reduced glutathione; Leukotriene A4


organism: Neisseria meningitidis

substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


assay_conditions_summary: pH 7.8; 298.15 K; cofactor ATP


organism: Lysinibacillus sphaericus


organism: Mycobacterium tuberculosis

proof_lines: pH 7.5, temperature not specified in the publication


organism: Lactobacillus plantarum

substrate_name: L-Arabinose

substrate: L-Arabinose


enzyme_display_name: nitrogenase

organism: Unknown

enzyme: nitrogenase


enzyme_display_name: fruit bromelain

substrate_name: Nalpha-benzoyl-Arg amide

enzyme: fruit bromelain

substrate: Nalpha-benzoyl-Arg amide


enzyme_display_name: tubulin GTPase

substrate_name: GTP

enzyme: tubulin GTPase

substrate: GTP


substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication, versus UDP-N-acetyl-D-glucosamine

proof_lines: pH and temperature not specified in the publication, versus GnGnbi-PA

substrate: Unknown


enzyme_display_name: FAD:protein FMN transferase

organism: Vibrio cholerae

substrate_name: FAD

assay_conditions_summary: pH 9; cofactor FAD

proof_lines: pH 9.0, temperature not specified in the publication, mutant H257G

enzyme: FAD:protein FMN transferase

substrate: FAD


proof_lines: pH 9.0, temperature not specified in the publication, mutant H257K


assay_conditions_summary: pH 9

proof_lines: pH 9.0, temperature not specified in the publication, mutant H257E


proof_lines: pH 9.0, temperature not specified in the publication, mutant H257t


proof_lines: pH 9.0, temperature not specified in the publication, wild-type enzyme

proof_lines: above, pH 9.0, 22°C, recombinant wild-type enzyme


enzyme_display_name: lipoprotein lipase

substrate_name: triacylglycerol

proof_lines: from chylomicrons, pH 8.0, 37°C, wild-type enzyme

enzyme: lipoprotein lipase

substrate: triacylglycerol


proof_lines: from chylomicrons, pH 8.0, 37°C, mutant LPLS447X


enzyme_display_name: mannosyl-oligosaccharide glucosidase

organism: Aspergillus brasiliensis

proof_lines: in 20 mM sodium phosphate, pH 7.0, at 25°C

enzyme: mannosyl-oligosaccharide glucosidase


organism: Candida albicans

assay_conditions_summary: pH 7; 310.15 K; buffer pH 7

proof_lines: in 10 mM sodium phosphate buffer pH 7.0 and at 37°C


organism: Ananas comosus


proof_lines:
pH 8.0, 37°C
at pH 5.0 and 37Â°C
at pH 8.0 and 37°C


organism: Azotobacter vinelandii


substrate_name: SCN-

proof_lines: before and after reduction of the nitrogenase complex relatively slow reactions take place, which limits the rate of the Fe protein cycle

substrate: SCN-


organism: Gallus gallus


proof_lines:
Hep3B cells
HepG2 cells


organism: Rattus norvegicus

substrate_name: pyridylaminated acceptor substrate

proof_lines:
recombinant enzyme
with UDP-GlcNAc as donor substrate
with UDP-Glc as donor substrate

substrate: pyridylaminated acceptor substrate


enzyme_display_name: rubber cis-polyprenylcistransferase

organism: Ficus carica

substrate_name: farnesyl diphosphate

enzyme: rubber cis-polyprenylcistransferase

substrate: farnesyl diphosphate


organism: Ficus elastica

substrate_name: isopentenyl diphosphate

substrate: isopentenyl diphosphate


organism: Hevea brasiliensis


organism: Parthenium argentatum


enzyme_display_name: beta-glucoside kinase

organism: Klebsiella aerogenes

substrate_name: arbutin

assay_conditions_summary: pH 7.5; cofactor ATP

enzyme: beta-glucoside kinase

substrate: arbutin


organism: Klebsiella pneumoniae

substrate_name: Salicin

substrate: Salicin


organism: Bos taurus

substrate_name: triolein

proof_lines: 25°C, pH 8.5, hydrolysis of p-nitrophenyl butyrate

substrate: triolein


substrate_name: very-low-density lipoprotein

substrate: very-low-density lipoprotein


organism: Homo sapiens


substrate_name: Very-low-density lipoproteins

proof_lines:
enzyme from post-heparin plasma
soluble enzyme

substrate: Very-low-density lipoproteins


enzyme_display_name: granzyme A

substrate_name: Benzyloxycarbonyl-Lys-thiobenzyl ester

proof_lines:
human granzyme A
pH 7.5, 30°C

enzyme: granzyme A

substrate: Benzyloxycarbonyl-Lys-thiobenzyl ester


organism: Mus musculus

substrate_name: benzyloxycarbonyl-Lys thiobenzyl ester

substrate: benzyloxycarbonyl-Lys thiobenzyl ester


organism: Sus scrofa

proof_lines:
strong inhibition
strong specific inhibition


organism: Vigna radiata


assay_conditions_summary: pH 5

proof_lines:
fruit bromelain A
fruit bromelain B
fruit bromelain C


organism: Bromelia karatas

substrate_name: ovalbumin

substrate: ovalbumin


organism: Bromelia pinguin


proof_lines:
very slight inhibition
strong inhibition


organism: Entamoeba histolytica


organism: Escherichia coli

substrate_name: nigerose

substrate: nigerose


organism: Saccharomyces cerevisiae


substrate_name: GTP

assay_conditions_summary: pH 7

proof_lines: in 0.01 M sodium phosphate and 4 mM MgCl2, pH 7

substrate: GTP


proof_lines: tubulin in complex with T2R, ligand colchicine

proof_lines: tubulin in complex with T2R, ligand podophylotoxin


organism: Prosthecobacter sp.

proof_lines: activity of BtubB when mixed 1:1 with BtubA

proof_lines: activity of BtubB, the activity increases to 0.022 per sec when mixed 1:1 with BtubA


enzyme_display_name: leucoanthocyanidin reductase

organism: Desmodium uncinatum

enzyme: leucoanthocyanidin reductase


organism: Vitis vinifera


enzyme_display_name: (-)-borneol dehydrogenase

organism: Pseudomonas sp. TCU-HL1

substrate_name: (-)-borneol

proof_lines: refolded recombinant protein, pH 7, 25°C

enzyme: (-)-borneol dehydrogenase

substrate: (-)-borneol


enzyme_display_name: catechol O-methyltransferase

organism: Mycobacterium tuberculosis

substrate_name: S-Adenosyl-L-methionine; Caffeate

enzyme: catechol O-methyltransferase

substrate: S-Adenosyl-L-methionine; Caffeate


organism: Rattus norvegicus

substrate_name: S-Adenosyl-L-methionine; L-Adrenaline

proof_lines: enzyme from pooled liver S9, at pH 7.4 and 37°C

substrate: S-Adenosyl-L-methionine; L-Adrenaline


enzyme_display_name: N-terminal methionine N(alpha)-acetyltransferase NatF

organism: Arabidopsis thaliana

substrate_name: acetyl-CoA

assay_conditions_summary: cofactor CoA

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: acetyl-CoA


proof_lines: mutant P47A, pH not specified in the publication, temperature not specified in the publication

enzyme: N-terminal methionine N(alpha)-acetyltransferase NatF


proof_lines: SUMO-tagged wild-type, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant 181A, pH not specified in the publication, temperature not specified in the publication


enzyme_display_name: adenylate cyclase

substrate_name: ATP

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP

proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant K136A, 10 mM Mg2+

enzyme: adenylate cyclase

substrate: ATP


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant K332A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant K253A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain, 10 mM Mg2+


substrate_name: Unknown

proof_lines: pH 8.0, 37°C, versus ATP, wild-type catalytic domain Cya2-446

substrate: Unknown


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant W374A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant S103A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant T189A, 10 mM Mg2+


organism: Euglena gracilis

assay_conditions_summary: cofactor ATP


organism: Haloactinopolyspora alba

assay_conditions_summary: pH 10; 313.15 K; cofactor ATP


organism: Homo sapiens


verification_status: verified

assay_conditions_summary: 303.15 K; cofactor ATP

column_label: TABLE III; row Wild type AC; column KmATP (Mn2+)

normalized_value_unit_evidence: Per-metric normalized values and units are preserved in paper_metric_evidence.

raw_value_unit_evidence: Per-metric raw values and units are preserved in paper_metric_evidence; captured_primary_segment preserves the exact source transcription.

row_label: Wild type AC / Mn2+

table_label: TABLE III - Determination of kinetic parameters for the wild type AC in the presence of Mg2+ and Mn2+

status: verified


proof_lines: mutant F46A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant E97A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant I48A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant F180A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant Y115A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant Y50A, pH not specified in the publication, temperature not specified in the publication


enzyme_display_name: gentamicin 2'-N-acetyltransferase

organism: Mycolicibacterium smegmatis

substrate_name: neomycin

assay_conditions_summary: pH 6.5; 298.15 K; cofactor CoA

enzyme: gentamicin 2'-N-acetyltransferase

substrate: neomycin


substrate_name: sisomicin

substrate: sisomicin


enzyme_display_name: 1,3-alpha-oligoglucan phosphorylase

organism: Lachnoclostridium phytofermentans

substrate_name: kojibiose

proof_lines: pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate

proof_lines: pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate

proof_lines: pH 7.0, 30°C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose

enzyme: 1,3-alpha-oligoglucan phosphorylase

substrate: kojibiose


enzyme_display_name: thioglucosidase

substrate_name: H2O; Sinigrin

enzyme: thioglucosidase

substrate: H2O; Sinigrin


organism: Armoracia rusticana

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

proof_lines:
pH 5.5, 37°C
recombinant enzyme, pH 5.5, 37°C

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Anabaena sp.

assay_conditions_summary: pH 8.5; 318.15 K; cofactor ATP


assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

proof_lines: 37°C, pH 7.5, recombinant cyaB1 AC, in the presence of 2 mM Mn2+ and 0.1 mM cAMP

proof_lines: 37°C, pH 7.5, recombinant cyaB1 AC, in the presence of 10 mM Mg2+ and 0.1 mM cAMP


organism: Dictyostelium discoideum


organism: Escherichia coli

proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant W249A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant Y394A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant E185A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant E242A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant S106A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant R19A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant K260A, 10 mM Mg2+


proof_lines: pH 8.0, 37°C, recombinant catalytic domain mutant S113A, 10 mM Mg2+


enzyme_display_name: (R,R)-butanediol dehydrogenase

organism: Clostridium autoethanogenum

enzyme: (R,R)-butanediol dehydrogenase


smiles: CCC(C)=O


substrate_name: acetone

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

substrate: acetone


substrate_name: NADPH

substrate: NADPH


substrate_name: (R)-acetoin

substrate: (R)-acetoin


organism: Cupriavidus necator

substrate_name: NADH; H+; Acetoin

assay_conditions_summary: cofactor NADPH

substrate: NADH; H+; Acetoin


organism: Mycobacterium sp.


organism: Paenibacillus polymyxa

assay_conditions_summary: pH 8; 295.15 K; cofactor NAD+


organism: Pseudomonas aeruginosa


organism: Rhodococcus erythropolis

substrate_name: diacetyl

assay_conditions_summary: pH 10; 318.15 K; cofactor NAD+

proof_lines: with diacetyl as cosubstrate, at pH 10.0 and 45°C

substrate: diacetyl


organism: Saccharomyces cerevisiae

substrate_name: NAD+; (R,R)-Butane-2,3-diol

assay_conditions_summary: cofactor NAD+

substrate: NAD+; (R,R)-Butane-2,3-diol


substrate_name: Acetoin

smiles: CC(=O)C(C)O

substrate: Acetoin


substrate_name: NADH

substrate: NADH


assay_conditions_summary: cofactor NADH


substrate_name: glycolaldehyde

assay_conditions_summary: pH 7; 303.15 K; cofactor NADH

proof_lines: recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B

proof_lines: recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y

substrate: glycolaldehyde


substrate_name: acetaldehyde

substrate: acetaldehyde


enzyme_display_name: methionine gamma-lyase

organism: Citrobacter freundii

substrate_name: methiin

enzyme: methionine gamma-lyase

substrate: methiin


substrate_name: alliin

substrate: alliin


substrate_name: L-methionine

substrate: L-methionine


substrate_name: S-methyl-L-cysteine

substrate: S-methyl-L-cysteine


organism: Entamoeba histolytica

substrate_name: H2O; L-Methionine

substrate: H2O; L-Methionine


proof_lines:
36°C, pH 6.8, recombinant EhMGL2
36°C, pH 6.8, recombinant EhMGL1


substrate_name: trifluoromethionine

substrate: trifluoromethionine


proof_lines: non-cleavable caspase-8 + 0.001 mM FLIP, 37°C, pH 7.4, in the presence of 0.7 M sodium citrate

proof_lines: cleaved caspase-8, 37°C, pH 7.4, in the presence of 0.7 M sodium citrate


enzyme_display_name: memapsin 1

substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication

proof_lines: pH 6.2, temperature not specified in the publication

proof_lines: pH 4, temperature not specified in the publication

enzyme: memapsin 1

substrate: Unknown


enzyme_display_name: creatinase

organism: Actinobacillus sp.

substrate_name: Creatine

proof_lines: parameter calculated for the bioelectrode, depends on the electrode material and the enzyme immobilization process, indicates that nanocomposite matrix has a high affinity to immobilize CAH enzyme, the small Km value indicates a high enzyme

enzyme: creatinase

substrate: Creatine


organism: Glutamicibacter nicotianae


organism: Pseudomonas putida

proof_lines: pH 7.7, 50°C, creatinase in yeast spores with OSW2 deletion


proof_lines:
pH 7.7, 50°C, soluble enzyme
Raw SKiD Env: Temp=50°C, pH=7.7


proof_lines: pH 7.7, 50°C, creatinase in wild-type yeast spores


proof_lines: pH 7.7, 50°C, creatinase in yeast spores with DIT1 deletion


proof_lines:
wild-type
wild-type, pH 7.2, 30°C


substrate_name: DL-homocysteine

substrate: DL-homocysteine


substrate_name: L-cysteine

substrate: L-cysteine


organism: Fusobacterium nucleatum subsp. nucleatum

proof_lines:
pH 7.6, 37°C
Raw SKiD Env: Temp=37°C, pH=7.6


organism: Micromonospora echinospora

proof_lines:
pH 8.0, 23°C
Raw SKiD Env: Temp=23°C, pH=8


organism: Porphyromonas gingivalis


enzyme_display_name: guanylate cyclase

organism: Catenaria anguillulae

substrate_name: GTP

proof_lines: pH 7.5, 22°C, wild-type enzyme, mutant enzyme E497K/C566D

proof_lines: pH 7.5, 22°C, wild-type enzyme, wild-type enzyme

enzyme: guanylate cyclase

substrate: GTP


organism: Dictyostelium discoideum


organism: Homo sapiens

proof_lines: pH and temperature not specified in the publication, mutant V902L enzyme in presence or absence of activator GCAP2

proof_lines: pH and temperature not specified in the publication, mutant V902L enzyme in presence of activator GCAP2

proof_lines: pH and temperature not specified in the publication, mutant V902L enzyme in presence of activator GCAP1


proof_lines: pH and temperature not specified in the publication, wild-type and mutant V902L enzymes in presence of activator GCAP1

proof_lines: pH and temperature not specified in the publication, wild-type enzyme in presence of activator GCAP1


enzyme_display_name: amino-acid racemase

organism: Bacillus subtilis

substrate_name: D-Lysine

enzyme: amino-acid racemase

substrate: D-Lysine


substrate_name: L-Lysine

substrate: L-Lysine


organism: Escherichia coli

substrate_name: L-Homoserine

substrate: L-Homoserine


substrate_name: L-homoserine

substrate: L-homoserine


organism: Latilactobacillus sakei

substrate_name: D-alanine

proof_lines: pH 10.0, 35°C, recombinant His-tagged enzyme

substrate: D-alanine


organism: Pyrococcus horikoshii

substrate_name: L-valine

proof_lines:
pH 7.0, 80°C, recombinant enzyme
pH 7.0, 80°C

substrate: L-valine


substrate_name: L-Alanine

substrate: L-Alanine


proof_lines:
pH 7.5, 37°C
at pH 7.5 and 37°C


proof_lines: pH 8.0, 37°C, recombinant wild-type enzyme


proof_lines: pH 8.0, 37°C, recombinant mutant Y114F/C116H


proof_lines: pH 8.0, 37°C, recombinant mutants K240R and K240M


organism: Thermococcus kodakarensis

substrate_name: D-methionine

substrate: D-methionine


organism: Thermococcus litoralis

substrate_name: D-leucine

substrate: D-leucine


enzyme_display_name: ribulose-phosphate 3-epimerase

organism: Bacillus methanolicus

substrate_name: D-ribulose 5-phosphate

enzyme: ribulose-phosphate 3-epimerase

substrate: D-ribulose 5-phosphate


substrate_name: D-Ribulose 5-phosphate

substrate: D-Ribulose 5-phosphate


organism: Oryza sativa


organism: Solanum tuberosum


organism: Spinacia oleracea


enzyme_display_name: steroid Delta-isomerase

organism: Pseudomonas putida

proof_lines: mutant enzyme Y30F/Y55F, at pH 7.0, temperature not specified in the publication

enzyme: steroid Delta-isomerase


proof_lines: mutant enzyme Y55F, at pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme Y30F/Y55F/Y115F, at pH 7.0, temperature not specified in the publication


proof_lines: wild type enzyme, at pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme Y30F, at pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme Y14F, at pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme Y115F, at pH 7.0, temperature not specified in the publication


organism: Sus scrofa

substrate_name: NAD+; 7alpha-Hydroxycholesterol

substrate: NAD+; 7alpha-Hydroxycholesterol


enzyme_display_name: complement factor I

proof_lines: mutant R130Q/R169Q, Vmax: 0.16 pmol/min/microgram protein

enzyme: complement factor I


proof_lines: mutant K51A/R62A, Vmax: 0.373 pmol/min/microgram protein


proof_lines: mutant K51A/R62A/L73A/L76A/F82A, Vmax: 0.085 pmol/min/microgram protein


proof_lines: mutant D207N/Q219A, Vmax: 0.044 pmol/min/microgram protein


enzyme_display_name: homospermidine synthase

organism: Unknown

substrate_name: putrescine

enzyme: homospermidine synthase

substrate: putrescine


organism: Sinapis alba

substrate_name: H2O; Sinigrin

proof_lines:
with dioxane as solvent
with acetonitrile as solvent
with 12.5 M methanol as solvent

substrate: H2O; Sinigrin


substrate_name: L-ethionine

substrate: L-ethionine


substrate_name: ATP

substrate: ATP


substrate_name: GTP

proof_lines: pH 7.5, 37°C, not stimulated recombinant enzyme

proof_lines: pH 7.5, 37°C, NO-stimulated recombinant enzyme

substrate: GTP


organism: Mus musculus

proof_lines: nonstimulated isozyme RetGC1, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C

proof_lines: nonstimulated isozyme RetGC2, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C

proof_lines: isozyme RetGC1, stimulated by guanylyl cyclase activating protein 2, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C


substrate_name: L-Leu

substrate: L-Leu


proof_lines: mutant K93Q/F94A, Vmax: 0.147 pmol/min/microgram protein


proof_lines: mutant K124Q/R150Q/F151A/K152Q, Vmax: 0.1 pmol/min/microgram protein


proof_lines: mutant L73A/L76A/F82A, Vmax: 0.053 pmol/min/microgram protein


proof_lines: mutant R150Q/F151A/K152Q, Vmax: 0.087 pmol/min/microgram protein


proof_lines: mutant D26N/K27Q/F29A/Q31A, Vmax: 0.834 pmol/min/microgram protein


proof_lines: mutant K182Q/R184Q, Vmax: 0.058 pmol/min/microgram protein


proof_lines: mutant V212A/L236A, Vmax: 2.126 pmol/min/microgram protein


proof_lines: mutant M220A/K221Q, Vmax: 0.01 pmol/min/microgram protein


enzyme_display_name: trimethyllysine dioxygenase

proof_lines: recombinant MBP-tagged enzyme, pH 7.0, 37°C

proof_lines: recombinant detagged enzyme, pH 7.0, 37°C

enzyme: trimethyllysine dioxygenase


organism: Rattus norvegicus


enzyme_display_name: methyl-branched lipid omega-hydroxylase

organism: Mycobacterium tuberculosis

substrate_name: H+; NADPH; O2; 15-Methyl-palmitic acid

proof_lines:
at pH 7.4 and 25°C
Raw SKiD Env: Temp=25°C, pH=7.4

enzyme: methyl-branched lipid omega-hydroxylase

substrate: H+; NADPH; O2; 15-Methyl-palmitic acid


organism: Bacillus sp. (in: firmicutes)

substrate_name: (R)-acetoin

proof_lines:
at pH 6.0 and 50°C
at pH 9.0 and 50°C

substrate: (R)-acetoin


organism: Bacillus subtilis subsp. subtilis

proof_lines: pH 7.0, 25°C, recombinant His-tagged enzyme


organism: Bacillus thuringiensis serovar kurstaki


organism: Clostridium autoethanogenum

substrate_name: acetaldehyde

smiles: CC=O

substrate: acetaldehyde


substrate_name: acetone

smiles: CC(C)=O

substrate: acetone


enzyme_display_name: glucan 1,4-alpha-maltotetraohydrolase

organism: Roseateles saccharophilus

substrate_name: maltotetraose

enzyme: glucan 1,4-alpha-maltotetraohydrolase

substrate: maltotetraose


organism: Homo sapiens

proof_lines: non-cleavable caspase-8 + 0.001 mM FLIP D297A, 37°C, pH 7.4, in the presence of 0.7 M sodium citrate


enzyme_display_name: candidapepsin

organism: Candida albicans

substrate_name: Unknown

enzyme: candidapepsin

substrate: Unknown


substrate_name: Lys-Pro-Ala-Arg-Phe-Nph-Arg-Leu

proof_lines:
pH 3.25, isoenzyme Sap2p
isoenzyme Sap2p

substrate: Lys-Pro-Ala-Arg-Phe-Nph-Arg-Leu


organism: Candida parapsilosis

assay_conditions_summary: pH 3.75; 310.15 K; buffer 150 mM sodium citrate

proof_lines: isoform Sapp1p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C

proof_lines: pH not specified in the publication, temperature not specified in the publication


proof_lines: isoform Sapp2p, in 150 mM sodium citrate buffer, pH 3.75, at 37°C


substrate_name: L-cysteine

proof_lines: pH 8.0, 37°C, recombinant mutants K240I and K240N

substrate: L-cysteine


substrate_name: H2O; L-Methionine

substrate: H2O; L-Methionine


organism: Streptomyces avermitilis

substrate_name: L-homocysteine

substrate: L-homocysteine


organism: Treponema denticola


organism: Trichomonas vaginalis


enzyme_display_name: L-3-cyanoalanine synthase

organism: Anthocharis cardamines

enzyme: L-3-cyanoalanine synthase


substrate_name: hydrogen sulfide

substrate: hydrogen sulfide


organism: Aporia crataegi


organism: Arabidopsis thaliana

substrate_name: L-Cysteine; Cyanide

substrate: L-Cysteine; Cyanide


organism: Colias croceus


organism: Glycine max

proof_lines:
recombinant enzyme, pH 9.0, 25°C
Raw SKiD Env: Temp=25°C, pH=9


organism: Gonepteryx rhamni


enzyme_display_name: type III protein arginine methyltransferase

substrate_name: acteyl-KKGGRGRKGKKY-amide

proof_lines: in the absence of KCl, at pH 9.5 and 25°C

proof_lines: in the presence of 130 mM KCl, at pH 9.5 and 25°C

substrate: acteyl-KKGGRGRKGKKY-amide


organism: Mus musculus

substrate_name: [histone H2B peptide 23-37]-L-arginine

proof_lines: with {[heat shock protein A8]-L-arginine469 as cosubstrate, at pH 8.0, temperature not specified in the publication

enzyme: type III protein arginine methyltransferase

substrate: [histone H2B peptide 23-37]-L-arginine


enzyme_display_name: D-alanine 2-hydroxymethyltransferase

organism: Aminobacter sp. AJ110403

substrate_name: tetrahydrofolate

enzyme: D-alanine 2-hydroxymethyltransferase

substrate: tetrahydrofolate


enzyme_display_name: ganglioside galactosyltransferase

organism: Campylobacter jejuni

substrate_name: GalNAcalpha-FCHASE

proof_lines: using fusion construct CgtBOH4384DELTA30-MalE (mutant with deletion of 30 amino acids from carboxy terminus + C-terminal MalE fusion)

enzyme: ganglioside galactosyltransferase

substrate: GalNAcalpha-FCHASE


organism: Bradyrhizobium japonicum

substrate_name: putrescine

substrate: putrescine


organism: Lachancea kluyveri


organism: Roseateles saccharophilus


organism: Pieris rapae


organism: Plutella xylostella


organism: Pseudomonas putida

substrate_name: O-Acetyl-L-serine; Cyanide

substrate: O-Acetyl-L-serine; Cyanide


organism: Solanum tuberosum


organism: Spinacia oleracea


organism: Spodoptera littoralis


organism: Tetranychus urticae

proof_lines: pH 8.5, 37°C, recombinant His-tagged enzyme


organism: Zygaena filipendulae


enzyme_display_name: adenylate cyclase

organism: Plasmodium falciparum

substrate_name: ATP

proof_lines: recombinant AC expressed from synthetic AC gene PfAC526-884

enzyme: adenylate cyclase

substrate: ATP


organism: Rattus norvegicus

proof_lines: sAC activity in cytosolic extracts in the presence of Mn2+


organism: Rhizobium etli


organism: Thermobifida halotolerans

assay_conditions_summary: pH 10; 318.15 K; cofactor ATP


organism: Trypanosoma cruzi


organism: Yersinia pestis

assay_conditions_summary: pH 7; 310.15 K; cofactor ATP


assay_conditions_summary: pH 8.5; 310.15 K; cofactor ATP

proof_lines: mutant enzyme E136A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme E136A, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C


proof_lines: mutant enzyme D55K, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme D55K, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C


organism: Catenaria anguillulae

substrate_name: GTP

substrate: GTP


organism: Homo sapiens

proof_lines:
recombinant sGC
Raw SKiD Env: Temp=-----, pH=-----


proof_lines: mutant T500A, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S502E, temperature not specified in the publication, pH not specified in the publication


proof_lines: wild-type, 37°C, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S473A, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant T500E, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S497A, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant T513A, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S473E, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S502A, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S506E, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S497E, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S510A, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S506A, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S487E, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S510E, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant S487A, temperature not specified in the publication, pH not specified in the publication


proof_lines: mutant T513E, temperature not specified in the publication, pH not specified in the publication


organism: Ipomoea nil


organism: Leishmania donovani

proof_lines:
recombinant enzyme
purified enzyme


substrate_name: D-ribulose 5-phosphate

substrate: D-ribulose 5-phosphate


enzyme_display_name: protein-glutamate O-methyltransferase

organism: Unknown

substrate_name: S-adenosyl-L-methionine

enzyme: protein-glutamate O-methyltransferase

substrate: S-adenosyl-L-methionine


organism: Streptococcus pyogenes


substrate_name: D-Ribulose 5-phosphate

proof_lines: RPE metal-free, stoichiometry of Zn2+: 0.1 ion per polypeptide, substrate 10 mM D-ribulose 5-phosphate

proof_lines: RPE as isolated + 0.5 mM ZnCl2 present in assay, stoichiometry of Zn2+: 0.9 ion per polypeptide, substrate 10 mM D-ribulose 5-phosphate

proof_lines: RPE as isolated, stoichiometry of Zn2+: 0.3 ion per polypeptide, substrate 10 mM D-ribulose 5-phosphate

substrate: D-Ribulose 5-phosphate


enzyme_display_name: aldose 1-epimerase

organism: Escherichia coli

substrate_name: alpha-D-Glucose

enzyme: aldose 1-epimerase

substrate: alpha-D-Glucose


proof_lines:
pH 8.0, 20°C
Raw SKiD Env: Temp=20°C, pH=8


enzyme_display_name: L-ribulose-5-phosphate 4-epimerase

substrate_name: L-Ribulose 5-phosphate

enzyme: L-ribulose-5-phosphate 4-epimerase

substrate: L-Ribulose 5-phosphate


organism: Comamonas testosteroni


proof_lines:
mutant D38E, pH 7.0, 29.9 °C
mutant D38E, pH 7.0, 5.4 °C
mutant D38E, pH 7.0, 25.0 °C


proof_lines:
Raw SKiD Env: Temp=25°C, pH=7
Raw SKiD Env: Temp=°C, pH=7


organism: Pseudomonas putida


proof_lines:
25°C, pH 7.0, wild-type enzyme
pH 7.0, 25°C, wild-type enzyme
wild-type, 25°C, pH 7.0


enzyme_display_name: creatinase

substrate_name: Creatine

enzyme: creatinase

substrate: Creatine


proof_lines: mutant D207N/Q219A/M220A/K221Q, Vmax: 0.032 pmol/min/microgram protein


proof_lines: mutant F29A/Q31A, Vmax: 0.854 pmol/min/microgram protein


proof_lines: mutant F94A/K182Q/R184Q, Vmax: 0.672 pmol/min/microgram protein


proof_lines: wild-type, Vmax: 0.09 pmol/min/microgram protein


enzyme_display_name: cephaeline 6'-O-methyltransferase

organism: Carapichea ipecacuanha

substrate_name: N-deacetylisoipecoside

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

proof_lines: kcat is 11.3x10(-3) sec(-1) with N-deacetylisoipecoside as substrate (PubMed:20061395). kcat is 20.4x10(-3) sec(-1) with 7-O-methyl-N-deacetylisoipecoside as substrate (PubMed:20061395). kcat is 0.28x10(-3) sec(-1) with cephaeline as substr

enzyme: cephaeline 6'-O-methyltransferase

substrate: N-deacetylisoipecoside


organism: Aquifex aeolicus


proof_lines: pH 7.5, 55°C, recombinant mutants L60A, F140A, and E113A


proof_lines: pH 7.5, 55°C, recombinant wild-type enzyme


enzyme_display_name: steroid Delta-isomerase

proof_lines:
pH 7.0, 25°C, mutant enzyme D99L
25°C, pH 7.0, mutant enzyme D99L

enzyme: steroid Delta-isomerase


enzyme_display_name: complement factor I

proof_lines: mutant V252A/I267A, Vmax: 1.284 pmol/min/microgram protein

enzyme: complement factor I


substrate_name: L-ribulose 5-phosphate

substrate: L-ribulose 5-phosphate


proof_lines: 25°C, pH 7.0, mutant enzyme Y14F/Y30F/Y55F/D99L


proof_lines: 25°C, pH 7.0, mutant enzyme Y14F/Y30F/Y55F


proof_lines: 25°C, pH 7.0, mutant enzyme Y14F/Y30F/Y55F/D99N


organism: Salmonella enterica

proof_lines: pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate, R340K mutant enzyme


proof_lines: pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate, R340A mutant enzyme


proof_lines: pH 8.5, 25°C, synthesis of 2-phosphonoacetaldehyde, R340K and R340A mutant enzymes


substrate_name: D-alanine

proof_lines: pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate

proof_lines: pH 8.5, 25°C, synthesis of 2-phosphonoacetaldehyde

substrate: D-alanine


proof_lines:
wild-type, 27°C, pH 7.4
27°C, pH 7.4, wild-type enzyme
wild-type, isoform 1, 27°C, pH 7.4


organism: Lactococcus lactis

substrate_name: alpha-D-xylose

substrate: alpha-D-xylose


substrate_name: alpha-D-glucose

substrate: alpha-D-glucose


enzyme_display_name: deoxyribodipyrimidine endonucleosidase

organism: Tequatrovirus T4

substrate_name: DNA

enzyme: deoxyribodipyrimidine endonucleosidase

substrate: DNA


enzyme_display_name: flavone apiosyltransferase

organism: Apium graveolens

substrate_name: apigenin 7-O-beta-D-glucoside

enzyme: flavone apiosyltransferase

substrate: apigenin 7-O-beta-D-glucoside


substrate_name: UDP-alpha-D-apiose

substrate: UDP-alpha-D-apiose


enzyme_display_name: (-)-borneol dehydrogenase

substrate_name: (-)-borneol

enzyme: (-)-borneol dehydrogenase

substrate: (-)-borneol


assay_conditions_summary: pH 7.5; 303.15 K; cofactor NAD+


organism: Bordetella pertussis

substrate_name: ATP

assay_conditions_summary: pH 8; 303.15 K; cofactor ATP

proof_lines: mutant enzyme R338A/D360A, at pH 8.0 and 30°C

substrate: ATP


assay_conditions_summary: 303.15 K; cofactor ATP


proof_lines:
wild type isoform VI, 30°C
wild type isoform V, 30°C


proof_lines: 37°C, pH 7.6, Zn2+-form after dialysis + 0.1 mM Zn2+, mutant enzyme H97N or D76N


proof_lines: 37°C, pH 7.6, Zn2+-form after dialysis + 0.1 mM Zn2+, mutant enzyme H95N

proof_lines: 37°C, pH 7.6, Zn2+-form after dialysis, mutant enzyme H95N

proof_lines: 22°C, pH 7.5, mutant enzyme H95N, activated by 0.1 mM ZnCl2


proof_lines: 37°C, pH 7.6, Zn2+-form after dialysis, mutant enzyme D76N

proof_lines: 37°C, pH 7.6, Zn2+-form after dialysis + 0.1 mM Zn2+, mutant enzyme D76N


organism: Mammalia

assay_conditions_summary: pH 7.4; 303.15 K; cofactor ATP

proof_lines: mutant VC1-A409P+IIC2, 30°C, pH 7.4, 10 mM MnCl, 0.1 mM forskolin


proof_lines: mutant VC1+IIC2-I1006V, 30°C, pH 7.4, 10 mM MnCl, 0.1 mM forskolin


proof_lines: mutant VC1-A406P+IIC2-I1006V, 30°C, pH 7.4, 10 mM MnCl, 0.1 mM forskolin


proof_lines: 22°C, pH 7.5, mutant enzyme Y229F, activated by 0.1 mM ZnCl2


proof_lines: 22°C, pH 7.5, wild-type enzyme, activated by 0.1 mM MgCl2

proof_lines: 37°C, pH 7.6, Zn2+-form after dialysis, wild-type


proof_lines: 22°C, pH 7.5, mutant enzyme H97N, activated by 0.1 mM ZnCl2

proof_lines: 37°C, pH 7.6, Zn2+-form after dialysis + 0.1 mM Zn2+, mutant enzyme H97N

proof_lines: 37°C, pH 7.6, Zn2+-form after dialysis, mutant enzyme H97N


substrate_name: dehydroepiandrosterone

substrate: dehydroepiandrosterone


proof_lines:
mutant Q105M, isoform 1, 27°C, pH 7.4
mutant Q105M, isoform 2, 27°C, pH 7.4


proof_lines: mutant T127M, same Km value as the wild-type

proof_lines: wild-type, Km of the mutants 31 - 1100 nM


organism: Aeromonas hydrophila

substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


proof_lines: mutants with recombinant plasmids expression of C- or N-domains


organism: Synechocystis sp.

mutation_signature: truncated Cya1

mutation_type: truncation

assay_conditions_summary: pH 7.5; 300.15 K; cofactor ATP


mutation_signature: truncated Cya1 / 50 mM NaHCO3 truncated Cya1

proof_lines: truncated Cya1, pH 7.5, 27°C, 50 mM NaHCO3


organism: Oryza sativa

substrate_name: L-cysteine

substrate: L-cysteine


substrate_name: HCN

proof_lines: recombinant enzyme from Saccharomyces cerevisiae

proof_lines: recombinant enzyme from Arabidopsis thaliana

substrate: HCN


enzyme_display_name: streptogrisin B

organism: Streptomyces griseus

substrate_name: Suc-AAPF-p-nitroanilide

enzyme: streptogrisin B

substrate: Suc-AAPF-p-nitroanilide


enzyme_display_name: methionine gamma-lyase

organism: Trichomonas vaginalis

substrate_name: cysteine

enzyme: methionine gamma-lyase

substrate: cysteine


proof_lines:
recombinant, MGL2 C116G
recombinant MGL2, C116G


organism: Homo sapiens


organism: Escherichia coli


enzyme_display_name: clavaminate synthase

organism: Streptomyces antibioticus

enzyme: clavaminate synthase


enzyme_display_name: imidazoleacetate 4-monooxygenase

organism: Pseudomonas sp.

substrate_name: imidazoleacetate

proof_lines: substrate: sodium diethyldithiocarbamate, polarographic method

enzyme: imidazoleacetate 4-monooxygenase

substrate: imidazoleacetate


enzyme_display_name: leucoanthocyanidin reductase

organism: Desmodium uncinatum

assay_conditions_summary: pH 7; 303.15 K; cofactor NADPH

enzyme: leucoanthocyanidin reductase


organism: Onobrychis viciifolia

substrate_name: NADPH

assay_conditions_summary: pH 6.8; 303.15 K; cofactor NADPH

substrate: NADPH


organism: Pseudotsuga menziesii

substrate_name: dihydroquercetin

substrate: dihydroquercetin


organism: Lavandula x intermedia


organism: Pseudomonas monteilii


enzyme_display_name: (R,R)-butanediol dehydrogenase

organism: Corynebacterium glutamicum

proof_lines:
at pH 10.5 and 30°C
at pH 7.0 and 30°C

enzyme: (R,R)-butanediol dehydrogenase


substrate_name: acetoin

assay_conditions_summary: pH 7; cofactor NADPH

proof_lines: cosubstrate NADPH, pH 7.0, temperature not specified in the publication

proof_lines: cosubstrate NADH, pH 7.0, temperature not specified in the publication

substrate: acetoin


organism: Klebsiella aerogenes

substrate_name: NAD+

substrate: NAD+


organism: Klebsiella pneumoniae

proof_lines:
enzyme DhaD, at pH 9.0 and 30°C
enzyme GldA, at pH 9.0 and 30°C


enzyme_display_name: demethylmacrocin O-methyltransferase

organism: Streptomyces fradiae

substrate_name: S-adenosylmethionine

proof_lines:
fixed substrate demethylmacrocin
fixed substrate demethyllactenocin
fixed substrate S-adenosylmethionine

enzyme: demethylmacrocin O-methyltransferase

substrate: S-adenosylmethionine


organism: Neurospora crassa

substrate_name: [cytochrome c]-L-lysine

proof_lines: residue 1-65 or residue 66-104 of horse heart cytochrome c

substrate: [cytochrome c]-L-lysine


organism: Saccharomyces cerevisiae

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


organism: Triticum aestivum

substrate_name: [apocytochrome c]-L-lysine

substrate: [apocytochrome c]-L-lysine


organism: Paenibacillus polymyxa

substrate_name: NADH

proof_lines:
pH 7.0, 30°C
(S)-acetoin, pH 7.0, 30°C

substrate: NADH


organism: Pyrococcus furiosus


enzyme_display_name: protein-glutamate O-methyltransferase

organism: Bacillus subtilis

proof_lines:
MCP methyltransferase I
MCP methyltransferase II

enzyme: protein-glutamate O-methyltransferase


organism: Bos taurus

substrate_name: [histone]-L-glutamate

substrate: [histone]-L-glutamate


organism: Homo sapiens

substrate_name: Unknown

substrate: Unknown


organism: Salmonella enterica subsp. enterica serovar Typhimurium

substrate_name: protein L-glutamate

substrate: protein L-glutamate


enzyme_display_name: fluorothreonine transaldolase

organism: Streptomyces sp. MA37

substrate_name: phenylacetaldehyde

enzyme: fluorothreonine transaldolase

substrate: phenylacetaldehyde


enzyme_display_name: D-alanine 2-hydroxymethyltransferase

organism: Pseudomonas sp.

substrate_name: L-tetrahydrofolate

proof_lines: pH 7.5, calculated on the basis of one isomer

enzyme: D-alanine 2-hydroxymethyltransferase

substrate: L-tetrahydrofolate


enzyme_display_name: methylmalonyl-CoA carboxytransferase

organism: Propionibacterium freudenreichii subsp. shermanii

substrate_name: pyruvate

assay_conditions_summary: cofactor CoA

enzyme: methylmalonyl-CoA carboxytransferase

substrate: pyruvate


organism: Propionibacterium sp.

substrate_name: oxaloacetate

substrate: oxaloacetate


enzyme_display_name: 2-hydroxy-3-oxoadipate synthase

organism: Mycobacterium tuberculosis

substrate_name: glyoxylate

enzyme: 2-hydroxy-3-oxoadipate synthase

substrate: glyoxylate


organism: Mycolicibacterium phlei


organism: Rattus norvegicus


organism: Sus scrofa


enzyme_display_name: DNA beta-glucosyltransferase

organism: Tequatrovirus T4

substrate_name: DNA containing 2 5-hydroxymethyl cytosine residues

proof_lines: substrate 5-hydroxymethyl cytosine containing DNA, pH 7.9, 25°C

enzyme: DNA beta-glucosyltransferase


organism: Candida albicans


substrate_name: synthetic peptide based on a mucin-like domain in alpha-dystroglycan


organism: Saccharomyces cerevisiae

substrate_name: dolichyl phosphate D-mannose

substrate: dolichyl phosphate D-mannose


enzyme_display_name: CO-methylating acetyl-CoA synthase

organism: Carboxydothermus hydrogenoformans

substrate_name: acetyl-CoA

assay_conditions_summary: pH 7.2; 298.15 K; cofactor CoA

proof_lines: pH 7.2, 25°C, recombinant ACSCh732, acetyl-CoA/CO exchange activity

proof_lines: pH 7.2, 25°C, recombinant ACSChDELTAN, acetyl-CoA/CO exchange activity

proof_lines: exchange activity: C14 from the carboxyl group of acetyl-CoA with C12 from CO

enzyme: CO-methylating acetyl-CoA synthase

substrate: acetyl-CoA


organism: Clostridioides difficile

substrate_name: HS-CoA

proof_lines: at 1 atm CO, pH not specified in the publication, temperature not specified in the publication

substrate: HS-CoA


organism: Methanosarcina thermophila

proof_lines: pH 7.2, 25°C, recombinant ACSD beta, acetyl-CoA/CO exchange activity


organism: Neomoorella thermoacetica

substrate_name: CoA

assay_conditions_summary: pH 7.3; 295.15 K; cofactor CoA

proof_lines: pH 7.3, 22°C, acetyl-CoA synthesis from methyl iodide

proof_lines: pH 7.0, 25°C, under anaerobic conditions, noncompetitive with respect to acetyl-CoA

substrate: CoA


enzyme_display_name: NDP-glucose--starch glucosyltransferase

organism: Gracilaria tenuistipitata

substrate_name: glycogen

assay_conditions_summary: cofactor ADP

proof_lines: UDP-glucose utilizing enzyme in presence of 0.5 M citrate

proof_lines: UDP-glucose utilizing enzyme in absence of citrate

enzyme: NDP-glucose--starch glucosyltransferase

substrate: glycogen


organism: Guillardia theta

substrate_name: ADP-glucose

assay_conditions_summary: pH 6.5; 328.15 K; cofactor ADP

substrate: ADP-glucose


organism: Oryza sativa

proof_lines: enzyme monomer, pH not specified in the publication, temperature not specified in the publication

proof_lines: enzyme oligomer, pH not specified in the publication, temperature not specified in the publication


organism: Pyrococcus furiosus

substrate_name: dTDP-glucose

substrate: dTDP-glucose


enzyme_display_name: flavone apiosyltransferase

organism: Petroselinum crispum

enzyme: flavone apiosyltransferase


enzyme_display_name: ganglioside galactosyltransferase

organism: Gallus gallus

substrate_name: UDP-galactose

enzyme: ganglioside galactosyltransferase

substrate: UDP-galactose


organism: Lithobates pipiens


organism: Neisseria meningitidis

substrate_name: UDP-N-acetyl-D-glucosamine

proof_lines:
UDP-N-acetyl-D-glucosamine as donor
UDP-N-acetyl-D-galactosamine as donor

substrate: UDP-N-acetyl-D-glucosamine


enzyme_display_name: cephaeline 6'-O-methyltransferase

organism: Carapichea ipecacuanha

substrate_name: S-adenosyl-L-methionine

enzyme: cephaeline 6'-O-methyltransferase

substrate: S-adenosyl-L-methionine


enzyme_display_name: monacolin J acid methylbutanoate transferase

organism: Aspergillus terreus

assay_conditions_summary: pH 7.9; 298.15 K; cofactor CoA

proof_lines: pH and temperature not specified in the publication


substrate_name: monacolin J acid

proof_lines: mutant I4N/A9V/K26E/R28S/I35L/N43R/D96R/S109C/A123P/M157V/S164G/S172N/L174F/A178L/N191G/L192I/Q241M/A247S/R250K/S256T/A261H/G275S/Q297G/L335M/L361M/V370I/A383V/N391S/H404K, pH not specified in the publication, temperature not specified in t

enzyme: monacolin J acid methylbutanoate transferase

substrate: monacolin J acid


proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


enzyme_display_name: very-long-chain ceramide synthase

organism: Arabidopsis thaliana

substrate_name: phytosphingosine

enzyme: very-long-chain ceramide synthase

substrate: phytosphingosine


organism: Mus musculus

substrate_name: D-erythro-sphinganine

proof_lines: pH 7.5, 37°C, recombinant enzyme, in presence of inhibitor sphingosine 1-phosphate

substrate: D-erythro-sphinganine


enzyme_display_name: glycogenin glucosyltransferase

organism: Neurospora crassa

substrate_name: UDP-glucose

enzyme: glycogenin glucosyltransferase

substrate: UDP-glucose


organism: Methanocaldococcus jannaschii

substrate_name: 5-phospho-alpha-D-ribose 1-diphosphate

proof_lines: pH 4.8, 70°C, uncompetitive inhibition under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate

proof_lines: pH 4.8, 70°C, uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate

substrate: 5-phospho-alpha-D-ribose 1-diphosphate


organism: Escherichia coli

proof_lines: 37°C, 50 mM HEPES, 50 mM KCl, 10 mM MgCl2 and pH 7.5, 0.1% Triton X-100


organism: Bacteroides thetaiotaomicron

substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


organism: Staphylococcus aureus

substrate_name: L-glutamate

substrate: L-glutamate


enzyme_display_name: L-ascorbate peroxidase

organism: Citrus x limon

enzyme: L-ascorbate peroxidase


organism: Gossypium hirsutum

substrate_name: L-ascorbate

substrate: L-ascorbate


organism: Nicotiana tabacum


organism: Oryza sativa

substrate_name: H2O2; L-Ascorbate

substrate: H2O2; L-Ascorbate


organism: Populus tomentosa


assay_conditions_summary: pH 7

proof_lines: pH 7.0, temperature not specified in the publication


enzyme_display_name: carotenoid isomerooxygenase

organism: Galleria mellonella

substrate_name: alpha-carotene

enzyme: carotenoid isomerooxygenase

substrate: alpha-carotene


organism: Mycobacterium tuberculosis


enzyme_display_name: flavonoid 3',5'-hydroxylase

organism: Billardiera heterophylla

substrate_name: apigenin

enzyme: flavonoid 3',5'-hydroxylase

substrate: apigenin


organism: Adineta vaga

substrate_name: DNA cytosine

proof_lines: pH 7.5, 22°C, enzyme mutant N4CMT79-324, in presence of 0.04 mM SAM

proof_lines: pH 7.5, 22°C, enzyme mutant N4CMT79-324, in presence of 0.2 mM SAM

substrate: DNA cytosine


organism: Aquifex aeolicus

substrate_name: guanine27 in tRNATyr

proof_lines: pH 7.5, 55°C, tRNA Tyr with wild-type sequence adenine26/guanine27 and mutation C43U

proof_lines: pH 7.5, 55°C, wild-type tRNATyr contains the sequence adenine26/guanine27

proof_lines: pH 7.5, 55°C, mutant tRNATyr in which the wilde-type adenine26/guanine27 sequence is substituted with guanine26/adenine27

substrate: guanine27 in tRNATyr


organism: Thalassiosira pseudonana


organism: Carapichea ipecacuanha

substrate_name: N-deacetylisoipecoside

proof_lines: pH 7.5, 30°C, cosubstrate: N-deacetylisoipecoside

substrate: N-deacetylisoipecoside


substrate_name: O-phospho-L-serine

substrate: O-phospho-L-serine


enzyme_display_name: [Src-family] C-terminal protein kinase

organism: Homo sapiens

substrate_name: KKKKEEIYFFF

proof_lines: in the presence of Mn2+, at pH 7.4 and 30°C

proof_lines: GST-tagged recombinant catalytic domain of the enzyme, at pH 7.4 and 30°C

enzyme: [Src-family] C-terminal protein kinase

substrate: KKKKEEIYFFF


enzyme_display_name: [RNA-polymerase]-subunit kinase

organism: Mus musculus

substrate_name: ATP

assay_conditions_summary: pH 6; cofactor ATP

proof_lines: recombinant enzyme, pH 6.0-7.5, temperature not specified in the publication

enzyme: [RNA-polymerase]-subunit kinase

substrate: ATP


assay_conditions_summary: cofactor ATP


substrate_name: CTP

substrate: CTP


substrate_name: N-acetylneuraminic acid

substrate: N-acetylneuraminic acid


organism: Oncorhynchus mykiss

proof_lines: cosubstrate N-glycolylneuraminic acid, pH 9, 25°C

proof_lines: cosubstrate N-acetylneuraminic acid, pH 9, 25°C


enzyme_display_name: chlorophyllase

organism: Brassica oleracea

substrate_name: chlorophyll a

proof_lines: wild type isoform CLH1, in 100 mM sodium phosphate, pH 7.4, at 40°C

enzyme: chlorophyllase

substrate: chlorophyll a


substrate_name: chlorophyll b

proof_lines: wild type isoform CLH2, in 100 mM sodium phosphate, pH 7.4, at 40°C

substrate: chlorophyll b


substrate_name: pheophorbide a methyl ester

substrate: pheophorbide a methyl ester


organism: Chlamydomonas reinhardtii


substrate_name: bacteriochlorophyll a

substrate: bacteriochlorophyll a


organism: Citrus sinensis

substrate_name: H2O; Chlorophyll a

substrate: H2O; Chlorophyll a


organism: Crocosphaera subtropica ATCC 51142


organism: Triticum aestivum


enzyme_display_name: Serratia marcescens nuclease

organism: Serratia marcescens

substrate_name: Unknown

enzyme: Serratia marcescens nuclease

substrate: Unknown


organism: Ginkgo biloba


organism: Oxynema acuminatum


organism: Solanum lycopersicum

substrate_name: pheophytin a

substrate: pheophytin a


proof_lines:
wild-type, pH 8, 25°C
wild-type, presence of Ca2+, pH 8, 25°C
wild-type, presence of EDTA, pH 8, 25°C


enzyme_display_name: carboxymethylcellulase

organism: Alicyclobacillus acidocaldarius

substrate_name: carboxymethyl cellulose

proof_lines: mutant D469V, pH 5.5, 60°C, calculated as amounts of reducing equivalents

enzyme: carboxymethylcellulase

substrate: carboxymethyl cellulose


proof_lines: wild-type, pH 5.5, 60°C, calculated as amounts of reducing equivalents


proof_lines: mutant D471E, pH 5.5, 60°C, calculated as amounts of reducing equivalents


proof_lines: mutant D468E, pH 5.5, 60°C, calculated as amounts of reducing equivalents


proof_lines: mutant D469A, pH 5.5, 60°C, calculated as amounts of reducing equivalents


proof_lines: mutant D468A, pH 5.5, 60°C, calculated as amounts of reducing equivalents


proof_lines: mutant D468V, pH 5.5, 60°C, calculated as amounts of reducing equivalents


proof_lines: mutant D469E, pH 5.5, 60°C, calculated as amounts of reducing equivalents


proof_lines: mutant D471A, pH 5.5, 60°C, calculated as amounts of reducing equivalents


organism: Thermoascus aurantiacus


organism: Ignisphaera aggregans

substrate_name: lupin galactan

substrate: lupin galactan


enzyme_display_name: cathepsin F

enzyme: cathepsin F


enzyme_display_name: phosphoribosyl-AMP cyclohydrolase

organism: Methanococcus vannielii

proof_lines: mutant enzyme H110A, pH and temperature not specified in the publication

enzyme: phosphoribosyl-AMP cyclohydrolase


proof_lines: mutant enzyme D92E, pH and temperature not specified in the publication


proof_lines: mutant enzyme D94E, pH and temperature not specified in the publication


proof_lines: wild-type enzyme, pH and temperature not specified in the publication


enzyme_display_name: mRNA 5'-phosphatase

organism: Saccharomyces cerevisiae

substrate_name: pppA-terminated poly(A)

enzyme: mRNA 5'-phosphatase

substrate: pppA-terminated poly(A)


organism: Semliki forest virus

substrate_name: a 5'-triphospho-[mRNA]

proof_lines: Nsp2-N (N-terminal fragment), pH 7.0, 25°C

substrate: a 5'-triphospho-[mRNA]


enzyme_display_name: prenyl-diphosphate phosphatase

organism: Methanosarcina mazei

substrate_name: prenyl diphosphate

enzyme: prenyl-diphosphate phosphatase

substrate: prenyl diphosphate


enzyme_display_name: phosphohistidine phosphatase

organism: Homo sapiens

assay_conditions_summary: pH 8; 310.15 K; buffer 50 mM

proof_lines: pH 8.0, 37°C, HEPES buffer (50 mM at pH 8.0) with 10 mM NaCl, 0.01% w/v Brij 35 and 0.5 mM DTT as additives

proof_lines: pH and temperature not specified in the publication

enzyme: phosphohistidine phosphatase


enzyme_display_name: adenylate cyclase

organism: Yersinia pestis

assay_conditions_summary: pH 8.5; 310.15 K; cofactor ATP

proof_lines: mutant enzyme C83A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme C83A, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C

enzyme: adenylate cyclase


proof_lines: mutant enzyme E10Q, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme E10Q, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C


proof_lines: mutant enzyme R63A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme R63A, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C


proof_lines: mutant enzyme F5A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme F5A, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C


proof_lines: mutant enzyme K76A, in the presence of 10 mM Mn2+, at pH 8.5 and 37°C

proof_lines: mutant enzyme K76A, in the presence of 20 mM Mg2+, at pH 8.5 and 37°C


assay_conditions_summary: pH 7; 310.15 K; buffer Tris; cofactor ATP

proof_lines: wild type enzyme, at pH 7.0, in Tris buffer at 37°C

proof_lines: wild type enzyme, at pH 7.5, in Tris buffer at 37°C

proof_lines: wild type enzyme, at pH 8.0, in Tris buffer at 37°C


enzyme_display_name: guanylate cyclase

organism: Bos taurus

substrate_name: GTP

proof_lines: purified recombinant sGC in presence of 3-ethyl-3-(ethylaminoethyl)-1-hydroxy-2-oxo-1-triazene

enzyme: guanylate cyclase

substrate: GTP


enzyme_display_name: D-sedoheptulose-7-phosphate isomerase

organism: Burkholderia pseudomallei

substrate_name: D-Sedoheptulose 7-phosphate

enzyme: D-sedoheptulose-7-phosphate isomerase

substrate: D-Sedoheptulose 7-phosphate


substrate_name: D-sedoheptulose 7-phosphate

proof_lines: mutant enzyme D61A, in 20 mM HEPES, pH 8.0, 10 mM MgCl2, 10 mM KCl, at 37°C

substrate: D-sedoheptulose 7-phosphate


proof_lines: mutant enzyme H64Q, in 20 mM HEPES, pH 8.0, 10 mM MgCl2, 10 mM KCl, at 37°C


proof_lines: wild type enzyme, in 20 mM HEPES, pH 8.0, 10 mM MgCl2, 10 mM KCl, at 37°C


organism: Escherichia coli

assay_conditions_summary: pH 8


organism: Helicobacter pylori

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Mycobacterium tuberculosis


proof_lines: pH 8.0, 22°C, recombinant wild-type enzyme


enzyme_display_name: clavaminate synthase

organism: Streptomyces clavuligerus

substrate_name: N-alpha-acetyl-L-arginine

proof_lines:
pH 7.0, 22°C
pH 7.0, 40°C
isozyme CS1, pH 7.0, 22°C

enzyme: clavaminate synthase

substrate: N-alpha-acetyl-L-arginine


enzyme_display_name: imidazoleacetate 4-monooxygenase

organism: Unknown

substrate_name: imidazoleacetate

enzyme: imidazoleacetate 4-monooxygenase

substrate: imidazoleacetate


enzyme_display_name: cortisol sulfotransferase

enzyme: cortisol sulfotransferase


enzyme_display_name: peptidyl-dipeptidase B

enzyme: peptidyl-dipeptidase B


organism: Clonorchis sinensis

proof_lines: isozyme CF-4, in 100 mM sodium phosphate (pH 6.5), at 37°C

proof_lines: isozyme CF-11, in 100 mM sodium phosphate (pH 5.5), at 37°C