enzyme_display_name: piperidine N-piperoyltransferase

organism: Piper nigrum

substrate_name: piperoyl-CoA

assay_conditions_summary: cofactor CoA

proof_lines: kcat is 1.01 sec(-1) with piperoyl-CoA as substrate (PubMed:33833371). kcat is 0.47 sec(-1) with piperidine as substrate (PubMed:33833371).

enzyme: piperidine N-piperoyltransferase

substrate: piperoyl-CoA


source_db: uniprot

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

protein_accession_database: UniProt

source_license: CC BY 4.0

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: kcat is 0.35 sec(-1) with piperoyl-CoA as substrate (PubMed:33833371). kcat is 0.27 sec(-1) with piperidine as substrate (PubMed:33833371).

source: uniprot


enzyme_display_name: phloroisovalerophenone synthase

organism: Humulus lupulus

kcat_display: Not reported

proof_lines: kcat is 0.21x10(-3) sec(-1) with 4-coumaroyl-CoA as substrate (PubMed:29760092). kcat is 0.24x10(-3) sec(-1) with malonyl-CoA as substrate (PubMed:29760092). kcat is 1.2x10(-3) sec(-1) with 4-coumaroyl-CoA as substrate (in the presence of C

enzyme: phloroisovalerophenone synthase


enzyme_display_name: sulfate adenylyltransferase (ADP)

organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

proof_lines: kcat is 93.8 sec(-1) with Ap4A as substrate.

enzyme: sulfate adenylyltransferase (ADP)


proof_lines: kcat is 81.2 sec(-1) with Ap4A as substrate.


enzyme_display_name: carbendazim hydrolysing esterase

organism: Nocardioides sp. (strain SG-4G)

substrate_name: carbendazim

proof_lines: kcat is 170 min(-1) with carbendazim as substrate.

enzyme: carbendazim hydrolysing esterase

substrate: carbendazim


enzyme_display_name: 1'-carboxy-chondrochloren decarboxylase

organism: Chondromyces crocatus

substrate_name: pre-chondrochloren B

proof_lines: kcat is 15.5 min(-1) with pre-chondrochloren B as substrate.

enzyme: 1'-carboxy-chondrochloren decarboxylase

substrate: pre-chondrochloren B


organism: Caenorhabditis elegans

substrate_name: adenosine 5'-phosphosulfate

source_db: brenda

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

assay_conditions_summary: pH 6.8; 303.15 K; buffer 0.1 M HEPES/KOH

proof_lines: in 0.1 M HEPES/KOH buffer (pH 6.8), 0.1 M potassium phosphate (pH 6.8), 0.1 M KH2PO4 (pH 4.2), at 30°C

substrate: adenosine 5'-phosphosulfate

source: brenda


organism: Saccharomyces cerevisiae

assay_conditions_summary: pH 8; 303.15 K; cofactor ADP

proof_lines: 4 mM adenosine 5-phosphosulfate, 30°C, pH 8


organism: Archaeoglobus fulgidus


organism: Methanopyrus kandleri

proof_lines:
1 M K2HPO4
pH 8.0, 65°C
0.3 M K2HPO4


organism: Methanosarcina barkeri


organism: Methanothermus fervidus

substrate_name: formyl-tetrahydromethanopterin

substrate: formyl-tetrahydromethanopterin


organism: Methylorubrum extorquens


enzyme_display_name: phosphohistidine phosphatase

organism: Homo sapiens

source_db: oed

enzyme: phosphohistidine phosphatase

source: oed


enzyme_display_name: 2,5-diketocamphane 1,2-monooxygenase

organism: Pseudomonas putida

substrate_name: NADH

assay_conditions_summary: cofactor NADH

proof_lines:
component E3
component E1
component E2

enzyme: 2,5-diketocamphane 1,2-monooxygenase

substrate: NADH


enzyme_display_name: cyanidin 3-O-galactosyltransferase

organism: Apium graveolens

substrate_name: cyanidin

enzyme: cyanidin 3-O-galactosyltransferase

substrate: cyanidin


organism: Camellia sinensis var. sinensis


organism: Daucus carota

substrate_name: peonidin

proof_lines:
pH 8.6, 30°C
cosubstrate pelargonidin, pH 8.6, 30°C
cosubstrate cyanidin, pH 8.6, 30°C

substrate: peonidin


enzyme_display_name: versatile peroxidase

organism: Pleurotus eryngii

source_db: skid

source_license: CC BY-NC-ND 4.0

proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=-----, pH=3.5
Raw SKiD Env: Temp=25°C, pH=3

enzyme: versatile peroxidase

source: skid


enzyme_display_name: beta-glucosidase

enzyme: beta-glucosidase


organism: Neotermes koshunensis

substrate_name: cellobiose

substrate: cellobiose


organism: Oryza sativa

substrate_name: cellotriose

km_display: Not reported

substrate: cellotriose


substrate_name: gentiobiose

substrate: gentiobiose


organism: Paenibacillus polymyxa


organism: Phanerodontia chrysosporium


organism: Pyrococcus furiosus


organism: uncultured microorganism

substrate_name: 4-nitrophenyl beta-D-fucopyranoside

proof_lines:
Raw SKiD Env: Temp=75°C, pH=5.5
Raw SKiD Env: Temp=60°C, pH=6

substrate: 4-nitrophenyl beta-D-fucopyranoside


enzyme_display_name: isocitrate dehydrogenase (NADP(+))

organism: Archaeoglobus fulgidus

substrate_name: NAD+

assay_conditions_summary: pH 8; 333.15 K; cofactor NADP+

proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=60°C, pH=8

enzyme: isocitrate dehydrogenase (NADP(+))

substrate: NAD+


organism: Escherichia coli

substrate_name: isocitrate

substrate: isocitrate


assay_conditions_summary: pH 6.8; 310.15 K; cofactor NADP+

proof_lines:
Raw SKiD Env: Temp=37°C, pH=6.8
Raw SKiD Env: Temp=21°C, pH=7.5
Raw SKiD Env: Temp=37°C, pH=7.2


assay_conditions_summary: pH 8; 310.15 K; cofactor NADP+

proof_lines:
Raw SKiD Env: Temp=37°C, pH=8
Raw SKiD Env: Temp=-----, pH=-----


organism: Mycobacterium tuberculosis

substrate_name: NADP+

assay_conditions_summary: pH 7.5; cofactor NADP+

substrate: NADP+


organism: Saccharomyces cerevisiae

substrate_name: NADPH

proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=-----, pH=7.5

substrate: NADPH


enzyme_display_name: sucrose synthase

organism: Nitrosospira multiformis

substrate_name: UDP

enzyme: sucrose synthase

substrate: UDP


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


organism: Solanum tuberosum

substrate_name: UDP-alpha-D-glucose

proof_lines: recombinant enzyme expressed in Saccharomyces cerevisiae

proof_lines: recombinant enzyme expressed in Escherichia coli

substrate: UDP-alpha-D-glucose


enzyme_display_name: anthranilate phosphoribosyltransferase

organism: Arabidopsis thaliana

substrate_name: anthranilate

mutation_type: substitution

enzyme: anthranilate phosphoribosyltransferase

substrate: anthranilate


proof_lines: Ki above 5 mM, mutant enzyme N215S, at pH 7.4 and 30°C


proof_lines: Ki above 5 mM, mutant enzyme N215S/P257T, at pH 7.4 and 30°C


organism: Juglans regia


substrate_name: Unknown

ki_unit: mM

proof_lines: pH and temperature not specified in the publication

substrate: Unknown


organism: Saccharolobus solfataricus


substrate_name: 5-phospho-alpha-D-ribose 1-diphosphate

proof_lines: 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A/P178A

substrate: 5-phospho-alpha-D-ribose 1-diphosphate


proof_lines: 60°C, pH 7.5, 2 mM Mg2+, mutant enzyme E224Q

proof_lines: 60°C, pH 7.5, 2 mM Mg2+,mutant enzyme E224Q


enzyme_display_name: creatine kinase

organism: Danio rerio

substrate_name: Creatine

assay_conditions_summary: 298.15 K; cofactor ATP

proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant S329A

enzyme: creatine kinase

substrate: Creatine


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant K36L


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant H267A


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant E185Q


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant A205S


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant Q46E


organism: Homo sapiens

substrate_name: N-ethylglycocyamine

substrate: N-ethylglycocyamine


substrate_name: ATP

assay_conditions_summary: cofactor ATP

substrate: ATP


substrate_name: cyclocreatine

substrate: cyclocreatine


organism: Acidithiobacillus caldus

proof_lines:
wild-type, immobilized, pH 8.5, 42°C
wild-type, pH 8.5, 42°C


proof_lines: mutant L637M/T640V, immobilized, pH 8.5, 42°C


assay_conditions_summary: pH 7; 310.15 K; cofactor ADP

proof_lines:
pH 7.0, 37°C
cosubstrate ADP, pH 7.0, 37°C
cosubstrate UDP, pH 7.0, 37°C


substrate_name: ADP

proof_lines:
pH 7.0, 37°C
cosubstrate UDP, pH 7.0, 37°C
cosubstrate ADP, pH 7.0, 37°C

substrate: ADP


organism: Beta vulgaris

substrate_name: sucrose

proof_lines:
pH 6.5, 35°C, SuSyI
pH 6.5, 35°C, SuSyII
pH 7.5, 35°C, SuSyI

substrate: sucrose


organism: Denitrovibrio acetiphilus


organism: Glycine max

substrate_name: ADP-alpha-D-glucose

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ADP

proof_lines: with UDP as cosubstrate, at pH 7.5 and 30°C

proof_lines: with UDP-alpha-D-glucose as cosubstrate, at pH 7.5 and 30°C

substrate: ADP-alpha-D-glucose


organism: Nitrosomonas europaea

assay_conditions_summary: pH 7; 333.15 K; cofactor ADP

proof_lines:
pH 7.0, 60°C, with ADP
pH 7.0, 60°C
pH 7.0, 60°C, with UDP-glucose


proof_lines: 60°C, pH 7.5, 2 mM Mg2+, mutant enzyme D223N

proof_lines: 60°C, pH 7.5, 2 mM Mg2+,mutant enzyme D223N


proof_lines: 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme R164A


proof_lines: 60°C, pH 7.5, 2 mM Mg2+, mutant enzyme K106Q


proof_lines: 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A


proof_lines: 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme R164A/H154A

proof_lines: 60°C, pH 7.5, 0.05 mM Mg2+,mutant enzyme R164A/H154A


proof_lines: +/-0.06, I36E/M47D double mutant, pH 7.2, 37°C, 0.01 microM subunit concentration


proof_lines: +/-0.04, wild-type, pH 7.2, 37°C, 0.01 microM subunit concentration

proof_lines: 60°C, pH 7.5, 2 mM Mg2+, wild-type enzyme

proof_lines: wild-type, in the presence of 2 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C


proof_lines: mutant F149S, in the presence of 0.05 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C


proof_lines: mutant D83G/F149S, in the presence of 2 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C

proof_lines: mutant D83G/F149S, in the presence of 0.05 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C


proof_lines: mutant D83G, in the presence of 2 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C


proof_lines: mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C


proof_lines: mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C


enzyme_display_name: procollagen-proline 4-dioxygenase

proof_lines:
isoform P4H4
isoform P4H1

enzyme: procollagen-proline 4-dioxygenase


proof_lines:
isoform P4H7
isoform P4H4


proof_lines:
isoform P4H1
MW: 10000-20000
isoform P4H7


organism: Caenorhabditis elegans


organism: Dictyostelium discoideum


substrate_name: DLDLEMLAPYIPMDDDF

proof_lines: N-terminal hydroxylation site of HIF-1alpha

substrate: DLDLEMLAPYIPMDDDF


substrate_name: DLDLEMLAPYIPMD

proof_lines: C-terminal hydroxylation site of HIF-1alpha

substrate: DLDLEMLAPYIPMD


substrate_name: DLDLEMLAPYIPMDD

substrate: DLDLEMLAPYIPMDD


proof_lines:
molecular weight 5000-10000 Da
molecular weight 20000-40000 Da


proof_lines:
type I and type II enzyme
type II enzyme
type I enzyme


proof_lines:
pH 7.0, 60°C
at pH 7.0 and 60°C


proof_lines: recombinant mutant enzyme H66P/D326A, at 25Â°C, pH not specified in the publication

proof_lines: mutant enzyme H66P/D326A, at pH 8.0 and 25°C


proof_lines: recombinant wild type enzyme, at 25Â°C, pH not specified in the publication


proof_lines: recombinant mutant enzyme H66P, at 25Â°C, pH not specified in the publication


proof_lines: recombinant mutant enzyme H66R, at 25Â°C, pH not specified in the publication


proof_lines: recombinant mutant enzyme D326E, at 25Â°C, pH not specified in the publication


proof_lines: recombinant mutant enzyme D326A, at 25Â°C, pH not specified in the publication


organism: Oryctolagus cuniculus


proof_lines: mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C


proof_lines: mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C


proof_lines: mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C


proof_lines: mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C


organism: Thermococcus kodakarensis


organism: Cryptosporidium muris

assay_conditions_summary: pH 8.5; 298.15 K; cofactor ATP

proof_lines: in form of Mg-ATP, with glycocyamine as substrate, at pH 8.5 and 25°C


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, wild-type


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant T304K


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant N146C


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant A189D


proof_lines: with substrate procollagen, isozymes P4H-I and P4H-III

proof_lines: with substrate procollagen, isozyme P4H-II


organism: Unknown


organism: Cucumis sativus

substrate_name: UDPglucose

proof_lines:
isozyme SS2
isozyme SS1
cosubstrate UDPfructose, isozyme SS2

substrate: UDPglucose


assay_conditions_summary: cofactor ADP


assay_conditions_summary: pH 8; 303.15 K; cofactor ATP

proof_lines: isoform III isolated after expression in Escherichia coli, pH 8.0, 30°C

proof_lines: isoform II isolated after expression in Escherichia coli, pH 8.0, 30°C

proof_lines: pH 7.0, ubiquitous mitochondrial isozyme


enzyme_display_name: furin

enzyme: furin


organism: Pseudomonas sp.

substrate_name: cephalosporin C

substrate: cephalosporin C


proof_lines: pH 8.5, 37°C, recombinant wild-type enzyme


proof_lines: pH 8.5, 37°C, immobilized wild-type enzyme


proof_lines:
pH 8.5, 37°C, free mutant enzyme
pH 8.5, 37°C, immobilized mutant enzyme


proof_lines: recombinant immobilized enzyme, pH 8.5, 37°C


organism: Pseudomonas sp. N176

proof_lines: pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS/L154betaY

proof_lines: pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS/F72betaR


substrate_name: glutaryl-7-aminocephalosporanic acid

proof_lines: pH 8.0, 25°C, recombinant wild-type enzyme

proof_lines: recombinant wild-type enzyme, pH and temperature not specified in the publication

substrate: glutaryl-7-aminocephalosporanic acid


proof_lines: mutant enzyme with mutation Y151F in the alpha-subunit and Q50N in the beta-subunit

proof_lines: mutant enzyme with mutation Y151F in alpha-subunit and mutation Q50N in beta-subunit


proof_lines:
wild-type enzyme
pH 7.5, 37°C, wild-type enzyme


proof_lines:
wild-type
wild-type, 25°C


enzyme_display_name: chymotrypsin

organism: Bos taurus

assay_conditions_summary: pH 7.6; 298.15 K; buffer 80 mM Tris HCl

proof_lines: presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)10, pH 7.6, 25°C

proof_lines: presence of 0.5 mM cetyltributylphosphonium bromide, pH 7.8, 27°C

proof_lines: 5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, pH 7.8, 27°C

enzyme: chymotrypsin


assay_conditions_summary: pH 8

proof_lines: pH 8, temperature not specified in the publication


proof_lines: wild-type, pH 8, temperature not specified in the publication


proof_lines: mutant S242T, pH 8, temperature not specified in the publication


proof_lines: mutant D236R, pH 8, temperature not specified in the publication


organism: Penaeus californiensis


enzyme_display_name: cathepsin L

organism: Homo sapiens

proof_lines: wild-type, pH 5.5, temperature not specified in the publication

proof_lines: wild-type, substrate: benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide, production rate of 7-amino-4-methylcoumarin measured, excitation and emission wavelengths of 370 and 460 nm, pseudo first order reaction conditions (inhibitor concent

enzyme: cathepsin L


proof_lines:
mutant enzyme T223V/DELTAE286-E289
mutant enzyme T223V/DELTAE286-N293


proof_lines: G139R mutant, substrate: benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide, production rate of 7-amino-4-methylcoumarin measured, excitation and emission wavelengths of 370 and 460 nm, pseudo first order reaction conditions (inhibitor conc


proof_lines: pH and temperature not specified in the publication

proof_lines: 25°C, pH not specified in the publication


proof_lines: mutant L69Y/M161L/G164A/A214L, pH 5.5, temperature not specified in the publication


organism: Leishmania mexicana


organism: Mus musculus

proof_lines: substrate: benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide, production rate of 7-amino-4-methylcoumarin measured, excitation and emission wavelengths of 370 and 460 nm, pseudo first order reaction conditions (inhibitor concentration at l


organism: Trichinella spiralis


proof_lines: pH 7.5, 37°C, mutant enzyme M271V/Q291K/T374S


organism: Periplaneta americana


organism: Rattus norvegicus

proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant V99M/M192R/G218R/V219E/S221T/T224/W/A226G, pH not specified in the publication, temperature not specified in the publication


organism: Severe acute respiratory syndrome coronavirus 2

proof_lines: native enzyme expressed by commercial vector, pH 7, 37°C

proof_lines: engineered enzyme expressed by modified vector, pH 7, 37°C


organism: Spodoptera frugiperda


enzyme_display_name: tissue kallikrein

enzyme: tissue kallikrein


substrate_name: Abz-FRSSR-Q-EDDnp

substrate: Abz-FRSSR-Q-EDDnp


proof_lines: pH 8.0, temperature not specified in the publication


proof_lines: pH 7.0, temperature not specified in the publication


organism: Trichoplusia ni


organism: Clonorchis sinensis


enzyme_display_name: acetoacetyl-CoA hydrolase

substrate_name: decanoyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: acetoacetyl-CoA hydrolase

substrate: decanoyl-CoA


enzyme_display_name: hydroxymethylglutaryl-CoA hydrolase

enzyme: hydroxymethylglutaryl-CoA hydrolase


enzyme_display_name: 2,3-bisphosphoglycerate 3-phosphatase

organism: Gallus gallus

substrate_name: inositol hexakisphosphate

enzyme: 2,3-bisphosphoglycerate 3-phosphatase

substrate: inositol hexakisphosphate


organism: Arabidopsis thaliana

substrate_name: ABA-beta-D-glucopyranosyl ester

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

substrate: ABA-beta-D-glucopyranosyl ester


enzyme_display_name: spermosin

organism: Halocynthia roretzi

substrate_name: Boc-Val-Pro-Arg-MCA

enzyme: spermosin

substrate: Boc-Val-Pro-Arg-MCA


organism: Enterobacter agglomerans


organism: Drosophila melanogaster

substrate_name: L-Dopa

substrate: L-Dopa


enzyme_display_name: phenylacetaldehyde synthase

organism: Petunia hybrida

substrate_name: L-phenylalanine

enzyme: phenylacetaldehyde synthase

substrate: L-phenylalanine


organism: Nocardioides sp. (strain KP7)


enzyme_display_name: cyanide hydratase

organism: Gibberella baccata

substrate_name: cyanide

enzyme: cyanide hydratase

substrate: cyanide


organism: Microdochium sorghi

substrate_name: cyanide (at pH 8 and 25 degrees Celsium)


enzyme_display_name: peptidylprolyl isomerase

organism: Homo sapiens

enzyme: peptidylprolyl isomerase


organism: Pseudomonas sp.

substrate_name: glutaryl-7-amino cephalosporanic acid

substrate: glutaryl-7-amino cephalosporanic acid


proof_lines: pH 8.0, 37°C, mutant enzyme S22P/T394P/D416Y/H417Y


proof_lines: pH 8.0, 37°C, mutant enzyme I44V/E49stop/D416Y/H417Y


proof_lines: mutant enzyme H296S/H309S, at pH 8.0 and 25°C


proof_lines: pH 8.0, 37°C, mutant enzyme A215Y/H296S/H309S


enzyme_display_name: tyrosinase

organism: Camellia sinensis

substrate_name: catechol

enzyme: tyrosinase

substrate: catechol


enzyme_display_name: carboxylesterase

organism: Rattus norvegicus

substrate_name: retinyl palmitate

enzyme: carboxylesterase

substrate: retinyl palmitate


organism: Pseudomonas sp. SY-77-1


substrate_name: adipyl-7-aminocephalosporanic acid

substrate: adipyl-7-aminocephalosporanic acid


enzyme_display_name: glutathione transferase

organism: Anopheles dirus

assay_conditions_summary: pH 6.5; buffer pH 6

proof_lines: mutant enzyme Y215E, in 0.1 M phosphate buffer pH 6.5

enzyme: glutathione transferase


proof_lines: mutant enzyme R214A, in 0.1 M phosphate buffer pH 6.5


substrate_name: Ethacrynic acid

proof_lines: mutant enzyme Y215A, in 0.1 M phosphate buffer pH 6.5

substrate: Ethacrynic acid


proof_lines: wild type enzyme, in 0.1 M phosphate buffer pH 6.5


proof_lines: mutant enzyme T174A, in 0.1 M phosphate buffer pH 6.5


proof_lines: mutant enzyme F212E, in 0.1 M phosphate buffer pH 6.5


proof_lines: mutant enzyme F212A, in 0.1 M phosphate buffer pH 6.5


proof_lines: mutant enzyme Q112A, in 0.1 M phosphate buffer pH 6.5


organism: Phanerodontia chrysosporium

substrate_name: 4-nitrophenyl butyrate

proof_lines: recombinant mutant S22A, pH 6.5, temperature not specified in the publication

substrate: 4-nitrophenyl butyrate


substrate_name: phenethyl isothiocyanate

substrate: phenethyl isothiocyanate


organism: Pinus tabuliformis


organism: Populus trichocarpa

substrate_name: sulforaphane

proof_lines: isoform GSTU45, pH not specified in the publication, at 30°C

substrate: sulforaphane


proof_lines:
wild-type enzyme
pH 7.5, 37°C, wild-type enzyme


enzyme_display_name: cellobiose dehydrogenase (acceptor)

organism: Gelatoporia subvermispora

substrate_name: cellobiose

proof_lines: 2,6-dichloroindophenol as electron acceptor

enzyme: cellobiose dehydrogenase (acceptor)

substrate: cellobiose


organism: Mycothermus thermophilus


substrate_name: lactose

substrate: lactose


substrate_name: 2,6-dichlorophenol indophenol

proof_lines: pH 4.5, reaction with cellobiose, wild-type enzyme

proof_lines: pH 4.5, electron acceptor: cytochrome c, wild-type enzyme

substrate: 2,6-dichlorophenol indophenol


proof_lines: pH 4.5, electron acceptor: cytochrome c, mutant enzyme M65H

proof_lines: pH 4.5, reaction with cellobiose, mutant enzyme M65H

proof_lines: pH 4.5, electron acceptor: 2,6-dichlorophenol indophenol, mutant enzyme M65H


substrate_name: cytochrome c

proof_lines: mutant F166Y, cellobiose oxidation with electron acceptors

substrate: cytochrome c


assay_conditions_summary: pH 4; 303.15 K; cofactor FAD

proof_lines: reduction rate increases with increasing concententrations of the electron acceptors methyl-1,4-benzoquinone or Fe3+

proof_lines: pH 4.5, 30°C, acceptor: 2,6-dichlorophenolindophenol


enzyme_display_name: laccase

organism: Escherichia coli

proof_lines: mutant M510L, pH 5.5, temperature not specified in the publication

enzyme: laccase


enzyme_display_name: formate dehydrogenase

organism: [Candida] boidinii

proof_lines: at pH 7.4, temperature not specified in the publication

proof_lines: in the absence of metal ion, at pH 7.4 and 25°C

proof_lines: in the presence of 0.003 mM W6+, at pH 7.4 and 25°C

enzyme: formate dehydrogenase


organism: Clostridium ljungdahlii


organism: Cupriavidus necator

substrate_name: NADH

proof_lines: pH and temperature not specified in the publication

substrate: NADH


organism: Moraxella sp.

substrate_name: formate

proof_lines: in the presence of 0.005 mM Na+, at pH 5.5 and 25°C

proof_lines: in the absence of metal ion, at pH 5.5 and 25°C

proof_lines: in 0.1 M potassium phosphate buffer pH 7.0, at 30°C

substrate: formate


enzyme_display_name: alcohol dehydrogenase

organism: Saccharomyces cerevisiae

substrate_name: ethanol

enzyme: alcohol dehydrogenase

substrate: ethanol


organism: Agroathelia rolfsii

substrate_name: maltose

substrate: maltose


organism: Amesia atrobrunnea


organism: Cerrena unicolor

proof_lines: with ferricytochrome c as cosubstrate, at pH 4.5 and 30°C

proof_lines: with 2,6-dichlorophenolindophenol as cosubstrate, at pH 4.5 and 30°C


organism: Coniophora puteana


proof_lines: mutant enzyme F216A, in 0.1 M phosphate buffer pH 6.5


proof_lines: mutant enzyme Y215H, in 0.1 M phosphate buffer pH 6.5


proof_lines: mutant enzyme F212H, in 0.1 M phosphate buffer pH 6.5


proof_lines: mutant enzyme Y215S, in 0.1 M phosphate buffer pH 6.5


organism: Pinus brutia


organism: Plasmodium falciparum

substrate_name: glutathione

substrate: glutathione


organism: Plasmodium vivax


organism: Plasmodium yoelii


organism: Populus trichocarpa

substrate_name: allyl-isothiocyanate

proof_lines: isoform GSTU16, pH not specified in the publication, at 30°C

substrate: allyl-isothiocyanate


organism: Salix babylonica


enzyme_display_name: feruloyl esterase

organism: Actinomyces sp. oral taxon 448

substrate_name: methyl caffeate

enzyme: feruloyl esterase

substrate: methyl caffeate


organism: Anaeromyces mucronatus

substrate_name: ethyl ferulate

substrate: ethyl ferulate


organism: Aspergillus awamori

substrate_name: alpha-naphthylbutyrate

substrate: alpha-naphthylbutyrate


substrate_name: alpha-naphthylcaprylate

substrate: alpha-naphthylcaprylate


organism: Aspergillus nidulans

substrate_name: methyl ferulate

proof_lines: recombinant His-tagged enzyme, pH 7.0, 37°C

substrate: methyl ferulate


organism: Aspergillus niger

proof_lines:
isoenzyme FAEB
FAEB


organism: Thermothelomyces myriococcoides


organism: Trametes sanguinea

proof_lines:
pH 4.5, 30°C, without polysaccharide
at pH 4.5 and 30°C


organism: Trametes versicolor


enzyme_display_name: glucuronosyltransferase

organism: Homo sapiens

substrate_name: propofol

proof_lines: UGT1A9/UGT2B7(mutant H35A)-CFP heterodimer, pH 7.4, 37°C

enzyme: glucuronosyltransferase

substrate: propofol


enzyme_display_name: purine-nucleoside phosphorylase

organism: Helicobacter pylori

substrate_name: guanosine

proof_lines:
pH 7.0, 25°C
pH 7.0, 25°C, with guanosine
pH 7.0, 25°C, with 7-methylguanosine

enzyme: purine-nucleoside phosphorylase

substrate: guanosine


organism: Anopheles dirus

proof_lines: mutant enzyme Y215F, in 0.1 M phosphate buffer pH 6.5


proof_lines: mutant enzyme F212Y, in 0.1 M phosphate buffer pH 6.5


substrate_name: Ethacrynic acid

proof_lines: mutant enzyme F212L, in 0.1 M phosphate buffer pH 6.5

substrate: Ethacrynic acid


proof_lines: mutant enzyme F212W, in 0.1 M phosphate buffer pH 6.5


organism: Physcomitrium patens

substrate_name: lactose

assay_conditions_summary: pH 6; cofactor FAD

proof_lines: at pH 6.0, temperature not specified in the publication

substrate: lactose


organism: Thermothelomyces fergusii


assay_conditions_summary: pH 6; 303.15 K; buffer 100 mM McIlvaine

proof_lines: mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C


proof_lines: mutant enzyme 700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C


proof_lines: recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C


organism: Neurospora crassa


organism: Parageobacillus thermoglucosidasius

substrate_name: 4-nitrophenyl laurate

substrate: 4-nitrophenyl laurate


organism: Piromyces sp. 'equi'


organism: Pleurotus eryngii


organism: Pleurotus sapidus


organism: Pseudoalteromonas haloplanktis

substrate_name: methylumbelliferyl acetate

substrate: methylumbelliferyl acetate


organism: Rhizoctonia solani


organism: Schizophyllum commune

substrate_name: methyl 4-hydroxybenzoate

substrate: methyl 4-hydroxybenzoate


organism: Streptomyces cinnamoneus

proof_lines: pH not specified in the publication, 50°C


organism: Streptomyces olivochromogenes


organism: Streptomyces sp. TH-2

proof_lines: mutant 18PG, pH not specified in the publication, 50°C


organism: Aspergillus niger

substrate_name: ferulate

substrate: ferulate


proof_lines:
mutant D93G/S187F, pH 6.4, 40°C
pH 6.4, 40°C, mutant D93G/S187F


proof_lines:
pH 5.0, 55°C
pH 6.0, 40°C
isoenzyme FAEA


organism: Aspergillus oryzae


substrate_name: methyl 4-coumarate

assay_conditions_summary: pH 6

substrate: methyl 4-coumarate


substrate_name: alpha-naphthyl propionate

substrate: alpha-naphthyl propionate


substrate_name: methyl p-coumarate

substrate: methyl p-coumarate


organism: Aspergillus terreus

substrate_name: ferulic acid methyl ester

substrate: ferulic acid methyl ester


proof_lines: wild-type, pH not specified in the publication, 50°C


organism: Talaromyces funiculosus


organism: Talaromyces pinophilus

substrate_name: 4-nitrophenyl acetate

substrate: 4-nitrophenyl acetate


organism: Talaromyces stipitatus


organism: Thermobacillus xylanilyticus

substrate_name: ethyl ferulate

substrate: ethyl ferulate


organism: Thermochaetoides dissita

substrate_name: 4-nitrophenyl butanoate

substrate: 4-nitrophenyl butanoate


organism: Thermothelomyces thermophilus


organism: Thermothielavioides terrestris


organism: uncultured bacterium


substrate_name: 4-nitrophenyl ferulate

substrate: 4-nitrophenyl ferulate


enzyme_display_name: beta-glucosidase

organism: Trichoderma viride

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

enzyme: beta-glucosidase

substrate: 4-nitrophenyl beta-D-glucopyranoside


enzyme_display_name: trypsin

organism: Bos taurus

substrate_name: Unknown

enzyme: trypsin

substrate: Unknown


organism: Unknown


organism: Fusarium oxysporum

proof_lines:
pH 6.0, 45°C
45°C, pH 6.0, isoenzyme FAE-I
pH 7.0, 45°C


organism: Neocallimastix sp.

proof_lines:
40°C, pH 6.2, isoenzyme FAE-I
40°C, pH 7.0, isoenzyme FAE-II
FAE-II


organism: Streptomyces cinnamoneus

proof_lines:
isozyme R43, pH 7.0, 50°C
isozyme R18, pH 7.0, 50°C


substrate_name: methyl ferulate

substrate: methyl ferulate


organism: Homo sapiens

substrate_name: enhanced green fluorescent protein-T1

assay_conditions_summary: pH 7.4; 310.15 K; buffer 10 mM phosphate

proof_lines: in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C

proof_lines: pH 8.0, 37°C, uncleaved enzyme, nonsulfated

proof_lines: pH 8.0, 37°C, uncleaved enzyme, sulfated

substrate: enhanced green fluorescent protein-T1


organism: Butyrivibrio proteoclasticus

substrate_name: 4-nitrophenyl butyrate

proof_lines: pH and temperature not specified in the publication

substrate: 4-nitrophenyl butyrate


substrate_name: methyl sinapate

substrate: methyl sinapate


organism: Lentilactobacillus buchneri


organism: Limosilactobacillus fermentum


enzyme_display_name: alcohol dehydrogenase (NADP+)

organism: Thermococcus sp.

substrate_name: NADP+

assay_conditions_summary: pH 8.8; buffer 100 mM EPPS; cofactor NADP+

proof_lines: recombinant enzyme, with 90 mM ethanol as cosubstrate, in 100 mM EPPS buffer (pH 8.8)

proof_lines: recombinant enzyme, with 0.4 mM NADP+ as cosubstrate, in 100 mM EPPS buffer (pH 8.8)

proof_lines: native enzyme, with 90 mM ethanol as cosubstrate, in 100 mM EPPS buffer (pH 8.8)

enzyme: alcohol dehydrogenase (NADP+)

substrate: NADP+


organism: Aspergillus awamori


organism: Aspergillus nidulans


organism: Aspergillus niger


organism: Aspergillus usamii

substrate_name: ferulic acid 4-nitrophenyl ester

substrate: ferulic acid 4-nitrophenyl ester


organism: Bacillus amyloliquefaciens


organism: Burkholderia pyrrocinia


organism: Thermococcus guaymasensis

substrate_name: acetoin

assay_conditions_summary: pH 7.5; 353.15 K; cofactor NADP+

proof_lines:
cosubstrate: NADPH, pH 7.5, 80°C
cosubstrate: NADP+, pH 7.5, 80°C
cosubstrate: 2-butanone, pH 7.5, 80°C

substrate: acetoin


enzyme_display_name: procollagen-proline 4-dioxygenase

organism: Chlamydomonas reinhardtii

substrate_name: poly(L-proline)

enzyme: procollagen-proline 4-dioxygenase

substrate: poly(L-proline)


proof_lines:
mutant S78T/S87L
mutants S78T/S87L


organism: Geobacillus thermodenitrificans

substrate_name: NAD+

proof_lines:
cosubstrate octan-1-ol, pH 8, 60°C
pH 8, 60°C
cosubstrate octanal, pH 8, 60°C

substrate: NAD+


substrate_name: p-nitrobenzaldehyde

proof_lines:
mutant R311A
aldehyde reductase, mutant R311A
mutant enzyme R311A, at pH 7.0 and 25°C

substrate: p-nitrobenzaldehyde


substrate_name: p-Carboxybenzaldehyde

proof_lines:
aldehyde reductase, mutant R308A
mutant enzyme R308A, at pH 7.0 and 25°C

substrate: p-Carboxybenzaldehyde


organism: Kluyveromyces lactis

substrate_name: NADH

proof_lines: wild type enzyme, at 25°C, pH not specified in the publication

substrate: NADH


proof_lines: mutant enzyme A274F, at 25°C, pH not specified in the publication


substrate_name: NADPH

proof_lines: mutant enzyme G225A/A274F, at 25°C, pH not specified in the publication

substrate: NADPH


substrate_name: acetaldehyde

proof_lines: mutant enzyme G225A, at 25°C, pH not specified in the publication

substrate: acetaldehyde


organism: Mycobacterium sp.


organism: Pectobacterium atrosepticum

substrate_name: Butanal

assay_conditions_summary: pH 7

proof_lines: pH 7.0, temperature not specified in the publication

substrate: Butanal


organism: Scheffersomyces stipitis


proof_lines: substrate 5-hydroxymethylfurfural, pH 7.2, 30°C


organism: Vanderwaltozyma polyspora

assay_conditions_summary: pH 9.5; 303.15 K; cofactor NADP+

proof_lines: pH 9.5, 30°C, recombinant mutant S196A/F197W, with 2-hydroxytetrahydrofuran


proof_lines: pH 9.5, 30°C, recombinant mutant V127C, with 2-hydroxytetrahydrofuran


proof_lines: pH 9.5, 30°C, recombinant mutant V84I/Y127M, with 2-hydroxytetrahydrofuran


proof_lines: pH 9.5, 30°C, recombinant mutant F197W, with 2-hydroxytetrahydrofuran


proof_lines: pH 9.5, 30°C, recombinant mutant Y127C/S196A, with 2-hydroxytetrahydrofuran


proof_lines: pH 9.5, 30°C, recombinant mutant V127M, with 2-hydroxytetrahydrofuran


proof_lines: pH 9.5, 30°C, recombinant wild-type enzyme, with 2-hydroxytetrahydrofuran


proof_lines: pH 9.5, 30°C, recombinant mutant S196A, with 2-hydroxytetrahydrofuran


enzyme_display_name: ribulose-bisphosphate carboxylase

organism: Thermosynechococcus vestitus

proof_lines: mutation P415A in subunit RbcL, plus mutation Y36N/R51H in subunit RbcS, pH 8.1, 25°C

enzyme: ribulose-bisphosphate carboxylase


proof_lines: mutation A398T/P415A in subunit RbcL, pH 8.1, 25°C


enzyme_display_name: monoamine oxidase

organism: Unknown

substrate_name: kynuramine

enzyme: monoamine oxidase

substrate: kynuramine


organism: Homo sapiens

proof_lines: pH 7.5, MAO-A incorporated into phospholipid nanodisks


organism: Rattus norvegicus

substrate_name: Phenylethylamine

proof_lines:
pH 7.5, 25°C, MAO-A
monoamine oxidase A
MAO-A

substrate: Phenylethylamine


substrate_name: benzylamine

proof_lines:
pH 7.5
MAO-B
above, MAO-B

substrate: benzylamine


enzyme_display_name: creatine kinase

substrate_name: Creatine

assay_conditions_summary: 298.15 K; cofactor ATP

proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant L36K

enzyme: creatine kinase

substrate: Creatine


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant A267H


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant D189A


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant Q185E


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant S205A


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant C146N


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant A329S


proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant E46Q


proof_lines:
pH 7.8, 30°C, enzyme variant D9
pH 7.8, 30°C, variant D11


proof_lines: mutant enzyme W230I/T354S/W430R, pH and temperature not specified in the publication


proof_lines: mutant enzyme W230I/W430R, pH and temperature not specified in the publication


proof_lines: mutant enzyme W230R/W430C/C214L, pH and temperature not specified in the publication


proof_lines: mutant enzyme W230I/T354S/W430R/M242R/Y365V, pH and temperature not specified in the publication


substrate_name: serotonin

substrate: serotonin


substrate_name: p-nitrophenylethylamine

substrate: p-nitrophenylethylamine


organism: Pseudomonas sp.


substrate_name: glutaryl-7-aminocephalosporanic acid

substrate: glutaryl-7-aminocephalosporanic acid


enzyme_display_name: acetylcholinesterase

organism: Mus musculus

substrate_name: Acetylcholine

enzyme: acetylcholinesterase

substrate: Acetylcholine


organism: Bos taurus

substrate_name: Unknown

substrate: Unknown


proof_lines:
pH 7.4, 37°C
pH 7.4, 38°C
in presence of 0.007 mM KCN


proof_lines:
monoamine oxidase B
isoform MAO B, at pH 7.4 and 37°C
pH 7.4, 30°C, reductive half-reaction


proof_lines:
pH 7.4, 37°C, enzyme from brain
pH 7.4, 37°C, enzyme from liver
inhibition of MAO-B


proof_lines: pH 7.0, 22°C, recombinant wild-type enzyme


organism: [Candida] boidinii

substrate_name: NADP+

assay_conditions_summary: pH 7.5; buffer 0.1 M potassium phosphate; cofactor NADP+

proof_lines: mutant enzyme D195Q/Y196P, in 0.1 M potassium phosphate buffer (pH 7.5)

substrate: NADP+


proof_lines: mutant enzyme D195Q, in 0.1 M potassium phosphate buffer (pH 7.5)


proof_lines: mutant enzyme D195N, in 0.1 M potassium phosphate buffer (pH 7.5)


substrate_name: NAD+

proof_lines: mutant enzyme D195Q/Y196S, in 0.1 M potassium phosphate buffer (pH 7.5)

substrate: NAD+


organism: Oryctolagus cuniculus

substrate_name: ATP

assay_conditions_summary: pH 9; 303.15 K; cofactor ATP

substrate: ATP


assay_conditions_summary: cofactor ATP

proof_lines:
ATP in form of MgATP2-
fusion protein CK-AK
oxidized form of creatine kinase


proof_lines:
wild-type enzyme
wild-type


substrate_name: poly(L-proline)

substrate: poly(L-proline)


organism: Cimex lectularius

proof_lines: 25°C, pH not specified in the publication


substrate_name: heroin

proof_lines: recombinant enzyme, pH and temperature not specified in the publication

substrate: heroin


substrate_name: acetylthiocholine

substrate: acetylthiocholine


organism: Thermoanaerobacter ethanolicus


assay_conditions_summary: 303.15 K; cofactor FAD


organism: Ctenocephalides felis

substrate_name: propionylthiocholine

proof_lines: isoform AChE2, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C

substrate: propionylthiocholine


proof_lines: isoform AChE1, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C


organism: Danio rerio

assay_conditions_summary: pH 8

proof_lines: muscle isoform, pH 8, temperature not specified in the publication

proof_lines: brain isoform, pH 8, temperature not specified in the publication


organism: Lucilia cuprina

proof_lines: native enzyme from head extract, at 22°C in 10 mM Na2HPO4/NaH2PO4, pH 7.4

proof_lines: recombinant enzyme, at 22°C in 10 mM Na2HPO4/NaH2PO4, pH 7.4


organism: Pardosa pseudoannulata

proof_lines: pH 7.0, temperature not specified in the publication


organism: Stomoxys calcitrans

assay_conditions_summary: pH 7.5; 298.15 K; buffer condition do not establish a strict measurement record

proof_lines: in potassium phosphate buffer (100 mM, pH 7.5), at 25°C


organism: Pseudomonas sp. 130

proof_lines: pH 8.0, 30°C, beta-subunitmutant enzyme H23D


proof_lines: pH 8.0, 30°C, beta-subunit mutant enzyme H23Q

proof_lines: pH 8.0, 30°C, beta-subunitmutant enzyme H23Q


proof_lines:
pH 8.0, 30°C, wild-type enzyme
pH 8.0, 37°C, wild-type enzyme


proof_lines: pH 8.0, 30°C, beta-subunitmutant enzyme E455Q


proof_lines: pH 8.0, 30°C, beta-subunitmutant enzyme E455D


proof_lines: pH 8.0, 30°C, beta-subunitmutant enzyme E455L

proof_lines: pH 8.0, 30°C, beta-subunit mutant enzyme E455L


organism: Aphis citricidus


proof_lines: pH 7.5, temperature not specified in the publication


proof_lines: pH 8.0, temperature not specified in the publication


organism: Chlamydomonas reinhardtii

substrate_name: poly(L-Pro)

substrate: poly(L-Pro)


organism: Homo sapiens


proof_lines:
pH 8.0, 27°C, wild-type
wild-type enzyme


organism: Glycine max

assay_conditions_summary: pH 7; buffer 0.1 M sodium phosphate; cofactor NAD+

proof_lines: mutant enzyme F290Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme F290T, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme F290Q, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme F290E, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


substrate_name: p-Carboxybenzaldehyde

proof_lines:
aldehyde reductase, mutant V299C
mutant enzyme V299C, at pH 7.0 and 25°C

substrate: p-Carboxybenzaldehyde


substrate_name: Succinic semialdehyde

proof_lines:
aldehyde reductase, mutant I298A
mutant enzyme I298A, at pH 7.0 and 25°C

substrate: Succinic semialdehyde


proof_lines:
aldehyde reductase, mutant I298C
mutant enzyme I298C, at pH 7.0 and 25°C


proof_lines:
aldehyde reductase, mutant H112Q
mutant enzyme H112Q, at pH 7.0 and 25°C


substrate_name: p-nitrobenzaldehyde

proof_lines:
wild-type aldehyde reductase
wild type enzyme, at pH 7.0 and 25°C

substrate: p-nitrobenzaldehyde


organism: Thermoanaerobacter brockii

substrate_name: Cyclopentanol

substrate: Cyclopentanol


proof_lines:
r-TBADH, wild-type, mutant D150C-TBADH
r-TBADH, wild-type


organism: Musca domestica

substrate_name: acetylthiocholine iodide

substrate: acetylthiocholine iodide


organism: Heterorhabditis bacteriophora

proof_lines:
pH 8.5, 35°C, isozyme AChEA
pH 8.5, 35°C, isozyme AChEB
pH 7.5, 37°C, isozyme AChE1A


substrate_name: Phenylethylamine

substrate: Phenylethylamine


substrate_name: serotonin

substrate: serotonin


organism: Mus musculus


proof_lines:
wild-type enzyme
wild-type
pH 7.0, 25°C, wild-type


organism: Cyprinus carpio

substrate_name: butyrylthiocholine iodide

proof_lines:
pH 7.6, 40°C, recombinant enzyme
pH 7.6, 40°C, native enzyme

substrate: butyrylthiocholine iodide


organism: Electrophorus electricus

substrate_name: Unknown

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: Unknown


organism: Schistosoma bovis


organism: Schistosoma haematobium


organism: Schistosoma mansoni


organism: Tetronarce californica


organism: Rattus norvegicus


proof_lines:
pH 7.0, 25°C, mutant Y337A
pH 8, 25°C, Y337A mutant


substrate_name: tryptamine

substrate: tryptamine


substrate_name: tyramine

substrate: tyramine


organism: Oryctolagus cuniculus

substrate_name: ATP

substrate: ATP


proof_lines: pH 9.0, 30°C, recombinant wild-type enzyme

proof_lines: wild-type containing both oxidized and reduced form


organism: Drosophila melanogaster


enzyme_display_name: hypoxanthine phosphoribosyltransferase

organism: Plasmodium falciparum

substrate_name: hypoxanthine

assay_conditions_summary: pH 7.4; 296.15 K; buffer modifiers but no exact primary kinetic table/value

proof_lines: activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C

proof_lines: activated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C

proof_lines: activated enzyme, with hypoxanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C

enzyme: hypoxanthine phosphoribosyltransferase

substrate: hypoxanthine


organism: Thermus thermophilus

substrate_name: guanine

substrate: guanine


organism: Brevibacillus laterosporus

substrate_name: glutaryl-7-aminocephalosporanic acid

substrate: glutaryl-7-aminocephalosporanic acid


organism: Pseudomonas sp.


assay_conditions_summary: pH 8.5; 310.15 K; buffer 100 mM Tris-HCl

proof_lines: wild-type enzyme and deletion mutant D4(212-ADLA-215), pH 8.5, 37°C


organism: Caldanaerobacter subterraneus subsp. tengcongensis


organism: Homo sapiens

proof_lines: pH 7.4, 22°C, recombinant mutant F36L, with hypoxanthine

proof_lines: pH 7.4, 22°C, recombinant mutant F36L, with xanthine


proof_lines: pH 7.4, 22°C, recombinant mutant F36K, with guanine

proof_lines: pH 7.4, 22°C, recombinant mutant F36K, with hypoxanthine


proof_lines: pH 7.4, 22°C, recombinant mutant F36G, with hypoxanthine

proof_lines: pH 7.4, 22°C, recombinant mutant F36G, with guanine


substrate_name: xanthine

proof_lines: pH 7.4, 22°C, recombinant wild-type enzyme, with guanine

substrate: xanthine


proof_lines: pH 7.4, 28°C, recombinant chimeric mutant enzyme, with substrate guanine

proof_lines: pH 7.4, 28°C, recombinant chimeric mutant enzyme with substrate hypoxanthine


organism: Mycobacterium tuberculosis

proof_lines: with hypoxanthine as cosubstrate, at pH 7.5 and 25°C

proof_lines: with guanine as cosubstrate, at pH 7.5 and 25°C


substrate_name: 5-phospho-alpha-D-ribose 1-diphosphate

proof_lines: pH 7.4, 37°C, recombinant nonactivated wild-type enzyme

proof_lines: pH 7.4, 37°C, recombinant IMP-activated wild-type enzyme

substrate: 5-phospho-alpha-D-ribose 1-diphosphate


proof_lines: pH 7.4, 37°C, recombinant nonactivated mutant W181Y

proof_lines: pH 7.4, 37°C, recombinant IMP-activated mutant W181Y


proof_lines: pH 7.4, 37°C, recombinant nonactivated mutant W181S

proof_lines: pH 7.4, 37°C, recombinant IMP-activated mutant W181S


proof_lines: pH 7.4, 37°C, recombinant IMP-activated mutant W181F

proof_lines: pH 7.4, 37°C, recombinant nonactivated mutant W181F


substrate_name: IMP

substrate: IMP


substrate_name: cephalosporin C

assay_conditions_summary: pH 8; buffer 100 mM Tris-HCl

proof_lines: mutant enzyme H309V, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication

substrate: cephalosporin C


proof_lines: mutant enzyme P295A, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication


proof_lines: mutant enzyme L677F, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication


proof_lines: mutant enzyme A675G, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication


organism: Trypanosoma brucei brucei

proof_lines: recombinant wild-type enzyme, pH 7.4, 25°C


organism: [Candida] boidinii

substrate_name: NADP+

proof_lines: mutant enzyme D195Q/Y196H, in 0.1 M potassium phosphate buffer (pH 7.5)

substrate: NADP+


proof_lines: mutant enzyme D195A, in 0.1 M potassium phosphate buffer (pH 7.5)


substrate_name: NAD+

proof_lines: mutant enzyme D195S, in 0.1 M potassium phosphate buffer (pH 7.5)

substrate: NAD+


proof_lines: enzyme tetramer containing the H501S mutant alpha subunit


proof_lines: wild-type enzyme tetramer and enzyme tetramer containing the H501S mutant alpha subunit


proof_lines: enzyme tetramer containing the histidine 501 to serine mutant alpha subunit


organism: Escherichia coli

proof_lines: 0.1 M Tris-HCl, 12 mM MgCl2, pH 7.4, 25°C

proof_lines: pH and temperature not specified in the publication


proof_lines:
at pH 7.4 and 37°C
at pH 7.4 and 25°C
above, pH 7.4, 25°C


organism: Legionella pneumophila

proof_lines: with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C


proof_lines: with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C


organism: Leishmania tarentolae

substrate_name: 5-phosphoribosyl 1-diphosphate

proof_lines: recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate

substrate: 5-phosphoribosyl 1-diphosphate


proof_lines: pH not specified in the publication, 37°C


proof_lines: pH 7.4, temperature not specified in the publication


organism: Schistosoma mansoni

proof_lines: pH 7.5, 37°C, recombinant isozyme HGPRT-1


enzyme_display_name: alpha-glucosidase

organism: Thermotoga maritima

substrate_name: 4-nitrophenyl alpha-D-galactopyranoside

enzyme: alpha-glucosidase

substrate: 4-nitrophenyl alpha-D-galactopyranoside


organism: Thermochaetoides thermophila

proof_lines: mutant enzyme G93H/I94Y/R259C, at pH 8.0, temperature not specified in the publication

proof_lines: mutant enzyme G93H/I94Y/R259C, at pH 7.0 and 25°C


proof_lines: mutant enzyme G193H/I94Y, at pH 8.0, temperature not specified in the publication


proof_lines: mutant enzyme G93H/I94R, at pH 8.0, temperature not specified in the publication

proof_lines: mutant enzyme G93H/I94R, at pH 7.0 and 25°C


proof_lines: wild type enzyme, with HCO3- as cosubstrate, at pH 5.0, temperature not specified in the publication


proof_lines: mutant enzyme N256D, at pH 8.0, temperature not specified in the publication


proof_lines: mutant enzyme N120C, at pH 7.0, temperature not specified in the publication


organism: Sulfolobus acidocaldarius

substrate_name: 4-nitrophenyl alpha-D-glucopyranoside

substrate: 4-nitrophenyl alpha-D-glucopyranoside


organism: Sulfurisphaera tokodaii

substrate_name: maltotriose

substrate: maltotriose


organism: Thermus thermophilus HB27

substrate_name: maltose

proof_lines:
wild-type, Vmax: 21 micromol/min mg
wild-type, Vmax: 357 micromol/min mg
wild-type, Vmax: 333 micromol/min mg

substrate: maltose


proof_lines: site-specific GK24 alpha-glucosidase mutant, Vmax: 3.4 micromol/min mg

proof_lines: site-specific mutant of HB27 alpha-glucosidase, Vmax: 1.9 micromol/min mg

proof_lines: site-specific GK24 alpha-glucosidase mutant, Vmax: 20 micromol/min mg


organism: Apis cerana indica

substrate_name: 4-nitrophenyl alpha-D-glucoside

substrate: 4-nitrophenyl alpha-D-glucoside


organism: Apis cerana japonica


organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4

substrate_name: maltopentaose

substrate: maltopentaose


organism: Ferroplasma acidiphilum


organism: Hordeum vulgare


organism: Saccharolobus solfataricus

proof_lines:
pH 4.5, 85°C, recombinant enzyme
pH 5.5, 85°C, recombinant enzyme


organism: Schizosaccharomyces pombe

substrate_name: kojibiose

substrate: kojibiose


organism: Anopheles aquasalis

proof_lines:
pH 6.5, 25°C, isozyme alphaGlu3
pH 6.5, 25°C, isozyme alphaGlu1
pH 6.5, 25°C, isozyme alphaGlu2


organism: Homo sapiens

substrate_name: kynuramine

assay_conditions_summary: pH 7.5; 298.15 K; buffer 50 mM phosphate

proof_lines: wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5)

substrate: kynuramine


proof_lines: mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5)


substrate_name: benzylamine

proof_lines: mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5)

substrate: benzylamine


organism: Aspergillus niger

substrate_name: p-nitrophenyl-alpha-D-glucopyranoside

sequence_source: UniProtKB:A2QJF7 live accession anchor; no sequence was loaded in this review.

substrate: p-nitrophenyl-alpha-D-glucopyranoside


organism: Candida albicans

sequence_source: UniProtKB:Q2V621 live accession anchor; contradicted enzyme/EC context.


sequence_source: UniProtKB:P10253 live accession anchor; no sequence was loaded in this review.


organism: Thermoplasma acidophilum

sequence_source: UniProtKB:Q9HLD0 live accession anchor; protein/EC annotation unavailable in the direct check.


organism: Levilactobacillus brevis

assay_conditions_summary: pH 7; 303.15 K; cofactor NADP+

proof_lines: reduction, recombinant mutant G37D, 30°C, pH 7.0

proof_lines: oxidation, cosubstrate NADP+, recombinant mutant G37D, 30°C, pH 7.0

proof_lines: oxidation, cosubstrate NAD+, recombinant mutant G37D, 30°C, pH 7.0


substrate_name: acetophenone

assay_conditions_summary: pH 7; 303.15 K; cofactor NADH

proof_lines: cofactor NADH, reduction, recombinant mutant G37Dm 30°C, pH 7.0

substrate: acetophenone


substrate_name: Phenylethanol

proof_lines: oxidation, cosubstrate NADP+, recombinant wild-type, 30°C, pH 7.0

proof_lines: cofactor NADPH, reduction, recombinant wild-type, 30°C, pH 7.0

substrate: Phenylethanol


organism: Bacillus subtilis

substrate_name: 7-aminocephalosporanic acid

proof_lines: D445G mutant, pH and temperature not specified in the publication

substrate: 7-aminocephalosporanic acid


proof_lines: E423/E442Q/D445N mutant, pH and temperature not specified in the publication


proof_lines: pH and temperature not specified in the publication


organism: Aspergillus awamori

substrate_name: alpha-naphthylbutyrate

substrate: alpha-naphthylbutyrate


organism: Trypanosoma cruzi

substrate_name: IMP

substrate: IMP


substrate_name: diphosphate

substrate: diphosphate


proof_lines: pH 7.5, 37°C, recombinant wild-type enzyme, with GMP


organism: Alkalihalophilus pseudofirmus


organism: Saccharomyces cerevisiae


organism: Glycine max

substrate_name: formate

assay_conditions_summary: pH 8; 310.15 K; cofactor NAD+

proof_lines: mutant enzyme A267M/I272V/F290N, at pH 8.0 and 37°C

substrate: formate


proof_lines: mutant enzyme A267M/I272V/F290D, at pH 8.0 and 37°C


organism: Pseudomonas sp.

substrate_name: cephalosporin C

substrate: cephalosporin C


organism: Trichomonas vaginalis

proof_lines:
isozyme 1, pH 4.5, 37°C
isozyme 2, pH 5.5, 37°C


substrate_name: NAD+

proof_lines: mutant enzyme F311N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication

substrate: NAD+


proof_lines: mutant enzyme F311D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


organism: Pyrococcus furiosus

substrate_name: NADP+

substrate: NADP+


organism: Penicillium citrinum

substrate_name: methyl 4-bromo-3-oxobutanoate

substrate: methyl 4-bromo-3-oxobutanoate


organism: Vanderwaltozyma polyspora

proof_lines: pH 9.5, 30°C, recombinant mutant V84I/Y127C, with 2-hydroxytetrahydrofuran


proof_lines: pH 9.5, 30°C, recombinant mutant V84I, with 2-hydroxytetrahydrofuran


organism: Camelus dromedarius

substrate_name: maltose

proof_lines:
pH 5.5, 37°C, isozyme MG6
pH 5.5, 37°C, isozyme MG4

substrate: maltose


enzyme_display_name: sucrose synthase

organism: Glycyrrhiza uralensis

substrate_name: UDP

mutation_signature: Δ9

mutation_type: deletion

proof_lines: mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C

proof_lines: mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C

enzyme: sucrose synthase

substrate: UDP


proof_lines: wild type enzyme isoform SUS1, at pH 5.5 and 50°C

proof_lines: wild type enzyme isoform SUS2, at pH 5.5 and 50°C


organism: Micractinium conductrix


organism: Aspergillus nidulans

substrate_name: maltotriose

substrate: maltotriose


organism: Leishmania donovani

substrate_name: guanine

substrate: guanine


organism: Bungarus fasciatus

substrate_name: acetylthiocholine

substrate: acetylthiocholine


organism: Plasmodium falciparum

substrate_name: xanthine

proof_lines: pH 7.4, 22°C, recombinant wild-type enzyme, with hypoxanthine

proof_lines: pH 7.4, 22°C, recombinant wild-type enzyme, with xanthine

substrate: xanthine


proof_lines: pH 7.4, 22°C, recombinant mutant L44F, with hypoxanthine

proof_lines: pH 7.4, 22°C, recombinant mutant L44F, with xanthine


organism: Bacteroides intestinalis

substrate_name: methyl ferulate

proof_lines: recombinant wild-type enzyme, pH 6.0, 45°C

substrate: methyl ferulate


organism: Mus musculus

substrate_name: Acetylcholine

substrate: Acetylcholine


proof_lines:
Y124C-acrylodan mutant
Y124C-SO3- mutant
Y124C-benzyl mutant


organism: Ferroplasma acidiphilum

substrate_name: sucrose

substrate: sucrose


substrate_name: maltotetraose

proof_lines: 60°C, pH not specified in the publication

substrate: maltotetraose


enzyme_display_name: furin

organism: synthetic construct

substrate_name: viral precursor protein E3E2

proof_lines: R64A mutant peptide of E3E2 from Asian Chikungunya virus strain

enzyme: furin

substrate: viral precursor protein E3E2


proof_lines: wild-type E3E2 from Asian Chikungunya virus strain


enzyme_display_name: cathepsin L

organism: Fasciola hepatica

proof_lines: mutant enzyme L69W, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C

enzyme: cathepsin L


proof_lines: wild type enzyme, at pH 5.5, in the presence of 10 mM cysteine-HCl, at 37°C


organism: Pseudomonas sp.

substrate_name: glutaryl-7-aminocephalosporanic acid

substrate: glutaryl-7-aminocephalosporanic acid


organism: Homo sapiens


enzyme_display_name: glucose-1-phosphate adenylyltransferase

organism: Escherichia coli

substrate_name: ATP

enzyme: glucose-1-phosphate adenylyltransferase

substrate: ATP


substrate_name: cephalosporin C

mutation_signature: deletion mutant D4(212-ADLA-215)

proof_lines: deletion mutant D4(212-ADLA-215), pH 8.5, 37°C

substrate: cephalosporin C


mutation_signature: deletion mutant D2(227-AM-228)

proof_lines: deletion mutant D2(227-AM-228), pH 8.5, 37°C


assay_conditions_summary: pH 8; 310.15 K; cofactor ATP


organism: Zea mays

substrate_name: alpha-D-glucose 1-phosphate

proof_lines: wild type BT2/SH2, 15 mM 3-phosphoglycerate

substrate: alpha-D-glucose 1-phosphate


proof_lines: mutant BT2/C114A, 15 mM 3-phosphoglycerate


enzyme_display_name: cellobiose dehydrogenase (acceptor)

organism: Phanerodontia chrysosporium

substrate_name: cellobiose

proof_lines: pH 4.5, 23°C, mutant enzyme H689N or mutant H689Q

enzyme: cellobiose dehydrogenase (acceptor)

substrate: cellobiose


substrate_name: lactose

substrate: lactose


assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

proof_lines: pH 7.4, 37°C, wild-type enzyme, in absence of 3-phosphoglycerate


proof_lines: mutant BT2/M172T, 15 mM 3-phosphoglycerate


proof_lines: wild-type, pH 7.4, 37°C, presence of phosphate


proof_lines: large subunit mutant D161G, 37°C, pH 7.4

proof_lines: large subunit mutant D161G, presence of 3-phosphoglycerate, 37°C, pH 7.4


proof_lines: large subunit mutant Q96G/D161G/A443R, 37°C, pH 7.4

proof_lines: large subunit mutant Q96G/D161G/A443R, presence of 3-phosphoglycerate, 37°C, pH 7.4


proof_lines: mutant BT2/S163F, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/T361C, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/C424V, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/H149S, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/D368S, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/P372A, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/V227R, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/Q213H, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/A508S, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/E438Q, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/V502T, 15 mM 3-phosphoglycerate


proof_lines: mutant BT2/C382F, 15 mM 3-phosphoglycerate


organism: Solanum tuberosum

assay_conditions_summary: 310.15 K; cofactor ATP


assay_conditions_summary: pH 7; 310.15 K; cofactor ATP

proof_lines: mutation P52L, large subunit, 37°C, pH 7.0


proof_lines: mutation K41R/T51K, large subunit, plus D143N, small subunit


proof_lines: mutation T51K, large subunit, plus D143N, small subunit


enzyme_display_name: feruloyl esterase

organism: Aspergillus niger

substrate_name: methyl sinapinate

enzyme: feruloyl esterase

substrate: methyl sinapinate


proof_lines:
pH 6.0, 37°C, native enzyme
pH 3.2
pH 6.1


substrate_name: methyl p-coumarate

substrate: methyl p-coumarate


proof_lines: mutation K41R, large subunit, plus D143N, small subunit


assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

proof_lines: large subunit mutant E38K/G101N, pH 7.5, 37°C


assay_conditions_summary: cofactor ATP


proof_lines: mutation D157L, large subunit, plus D143N, small subunit


organism: Unknown

substrate_name: ADP-glucose

assay_conditions_summary: cofactor ADP

substrate: ADP-glucose


proof_lines: pH 4.5, 23°C, mutant enzyme H689Q or mutant H689A

proof_lines: pH 4.5, 23°C, mutant enzyme H689Q or mutant enzyme H689A


assay_conditions_summary: pH 7; 310.15 K; cofactor ADP

proof_lines: mutation P52L, large subunit, plus mutation R350K, small subunit, 37°C, pH 7.0


proof_lines: mutation P52L, large subunit, plus mutation P112L, small subunit, 37°C, pH 7.0


proof_lines: mutation P52L, large subunit, plus mutation L46F, small subunit, 37°C, pH 7.0


organism: Arabidopsis thaliana

assay_conditions_summary: pH 7.9; cofactor ATP

proof_lines: pH 7.9, homotetramer APS1 in presence of 20 mM 3-phosphoglycerate

proof_lines: pH 7.9, APS1/APL4 in presence of 1 mM 3-phosphoglycerate

proof_lines: pH 7.9, APS1/APL2 in presence of 4 mM 3-phosphoglycerate


organism: Cereibacter sphaeroides

proof_lines: pH 8, 37°C, in absence of fructose 6-phosphate

proof_lines: pH 8, 37°C, in presence of fructose 6-phosphate

proof_lines: pH 8, 37°C, enzyme modified for 30 min with 5 mM 2,3-butanedione


organism: Nitrosomonas europaea

proof_lines:
pH 8.0, 37°C, presence of Mg2+
pH 8.0, 37°C, presence of Co2+
pH 8.0, 37°C, presence of Mn2+


organism: Rhodococcus jostii

proof_lines: hyperbolic behavior, Hill coefficient 1.3, presence of mannose 6-phosphate, pH 8.0, 37°C

proof_lines: hyperbolic behavior, Hill coefficient 0.9, pH 8.0, 37°C

proof_lines: hyperbolic behavior, Hill coefficient 1.1, presence of glucose 6-phosphate, pH 8.0, 37°C


organism: Solanum lycopersicum

substrate_name: diphosphate

assay_conditions_summary: pH 7.8; 310.15 K; cofactor ATP

proof_lines: pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L1 and small subunit S

proof_lines: pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L3 and small subunit S

substrate: diphosphate


proof_lines: large subunit mutant G101N, pH 7.5, 37°C


proof_lines: large subunit E370G/small subunit wild-type, pH 7.4, 37°C


assay_conditions_summary: pH 7.4; 298.15 K; cofactor ATP

proof_lines: heterodimer of small/large subunit, pH 7.4, 25°C

proof_lines: heterodimer of small/large subunit, presence of ATPgammaS, pH 7.4, 25°C


organism: Streptococcus mutans

proof_lines: S0.5 value, Hill coefficient 1.2, GlgC/GlgD complex, pH 8.0, 37°C

proof_lines: S0.5 value, Hill coefficient 1.5, subunit GlgC in presence of fructose 1,6-bisphosphate, pH 8.0, 37°C

proof_lines: S0.5 value, Hill coefficient 3.7, GlgC/GlgD complex, pH 8.0, 37°C


organism: Streptomyces coelicolor


proof_lines: chimeric enzyme, pH 7.4, 37°C, presence of 3-phosphoglycerate


proof_lines: mutant Q106A, S0.5 value, Hill coefficient 1.7, pH 8.0, 37°C

proof_lines: mutant Q106A, S0.5 value, Hill coefficient 1.2, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C

proof_lines: mutant Q106A, S0.5 value, Hill coefficient 1.3, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C


proof_lines: mutant Y114A, S0.5 value, Hill coefficient 0.3, pH 8.0, 37°C

proof_lines: mutant Y114A, S0.5 value, Hill coefficient 2.0, pH 8.0, 37°C

proof_lines: mutant Y114A, S0.5 value, Hill coefficient 0.2, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C


proof_lines: mutant W113A, S0.5 value, Hill coefficient 1.1, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C

proof_lines: mutant W113A, presence of 2 mM fructose 1,6-bisphosphate, pH 8.0, 37°C


proof_lines: mutant P103A, S0.5 value, Hill coefficient 1.1, pH 8.0, 37°C

proof_lines: mutant P103A, S0.5 value, Hill coefficient 1.5, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C

proof_lines: mutant P103A, S0.5 value, Hill coefficient 1.9, pH 8.0, 37°C


proof_lines: pH 7.9, APS1/APL4 in presence of 1 mM 3-phosphoglycerate


proof_lines: pH 7.5, 37°C, mutant TG-15, kinetic study


proof_lines: pH 7.6, 37°C, wild-type enzyme, comparison of Km of wild-type and mutant enzymes


proof_lines: mutant Q74A, presence of 2 mM fructose 1,6-bisphosphate, pH 8.0, 37°C


proof_lines: mutant R107A, S0.5 value, Hill coefficient 0.9, pH 8.0, 37°C

proof_lines: mutant R107A, S0.5 value, Hill coefficient 2.1, pH 8.0, 37°C

proof_lines: mutant R107A, S0.5 value, Hill coefficient 2.3, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C


proof_lines: mutant R115A, S0.5 value, Hill coefficient 1.7, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C

proof_lines: mutant R115A, S0.5 value, Hill coefficient 1.7, pH 8.0, 37°C

proof_lines: mutant R115A, S0.5 value, Hill coefficient 2.1, pH 8.0, 37°C


organism: Triticum aestivum

substrate_name: Unknown

assay_conditions_summary: pH 8

proof_lines: endosperm enzyme in presence of 1 mM 3-phosphoglycerate

proof_lines: pH 8.0, in absence of 0.25 mM 3-phosphoglycerate

proof_lines: endosperm enzyme in presence of 0.25 mM 3-phosphoglycerate

substrate: Unknown


proof_lines: chimeric mutant, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C

proof_lines: chimeric mutant, substrate ATP, pH 7.4, 37°C

proof_lines: chimeric mutant, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C


assay_conditions_summary: pH 7.5; cofactor ATP


proof_lines: pH 7.0, 37°C, mutant enzyme P295G, in the presence of a saturating concentration of fructose 1,6-bisphosphate,


proof_lines: pH 7.0, 37°C, mutant enzyme G336D, in the presence of a saturating concentration of fructose 1,6-bisphosphate


proof_lines: pH 7.0, 37°C, mutant enzyme P295N, in the presence of a saturating concentration of fructose 1,6-bisphosphate


enzyme_display_name: laccase

organism: Rhizoctonia solani

enzyme: laccase


organism: Toxicodendron vernicifluum


proof_lines: pH 7.0, 37°C, double mutant enzyme P295D/G336D


proof_lines: pH 7.0, 37°C, mutant enzyme P295Q, in the presence of a saturating concentration of fructose 1,6-bisphosphate


proof_lines: pH 7.0, 37°C, mutant enzyme P295D, in the presence of a saturating concentration of fructose 1,6-bisphosphate


proof_lines: pH 7.0, 37°C, mutant enzyme P295E, in the presence of a saturating concentration of fructose 1,6-bisphosphate


enzyme_display_name: peptidyl-dipeptidase A

organism: Homo sapiens

substrate_name: benzyloxycarbonyl-Phe-His-Leu

proof_lines: 37°C, pH 8.3, chimeric enzyme C417-579Ndom-ACE

enzyme: peptidyl-dipeptidase A

substrate: benzyloxycarbonyl-Phe-His-Leu


substrate_name: angiotensin I

substrate: angiotensin I


organism: Cavia porcellus

substrate_name: peptidyl L-proline

substrate: peptidyl L-proline


organism: Chlamydomonas reinhardtii

proof_lines: Mr 30000-40000 da, complex of recombinant subunits PHY-4.1 and PDI2

proof_lines: Mr 5000-10000 Da, complex of recombinant subunits PHY-4.1 and PDI2


organism: Gallus gallus

substrate_name: tert-butyloxycarbonyl-Pro-Pro-Gly-Pro

proof_lines: complete reaction with (Pro-Pro-Gly)5 as a peptide substrate

substrate: tert-butyloxycarbonyl-Pro-Pro-Gly-Pro


proof_lines: pH 7.8, 22°C, recombinant enzyme, HPLC-based assay

proof_lines: pH 7.8, 22°C, recombinant enzyme, fluoride-based assay


substrate_name: poly L-proline

proof_lines:
molecular weight 5000-10000 Da
molecular weight 30000-40000 Da
sequence present in extensin

substrate: poly L-proline


organism: Caenorhabditis elegans

substrate_name: 510-amino acid alpha1(I) collagen fragment


proof_lines: complex of recombinant subunits PHY-4.1 and PDI2


organism: Catharanthus roseus


organism: Struthio camelus australis


substrate_name: [Phe9,Arg10]angiotensin I

substrate: [Phe9,Arg10]angiotensin I


substrate_name: [Arg10]angiotensin I

substrate: [Arg10]angiotensin I


substrate_name: [Phe9]angiotensin I

substrate: [Phe9]angiotensin I


assay_conditions_summary: pH 8; 310.15 K; buffer Tris-NaCl buffer

proof_lines:
pH 8.0, 37°C, C-domain
pH 8.0, 37°C, N-domain
pH 7.4, 37°C


proof_lines: free acid, recombinant enzyme, and wild-type enzyme


organism: Drosophila melanogaster

substrate_name: hippuryl-His-Leu

proof_lines: C-catalytic domain of ACE, pH 7.5, temperature not specified in the publication

substrate: hippuryl-His-Leu


organism: Leishmania donovani


proof_lines:
pH 7.5, angiotensin-converting enzyme 2
pH, angiotensin-converting enzyme 2


organism: Paramecium bursaria Chlorella virus-1


organism: Phaseolus vulgaris

substrate_name: poly(L-Pro)

substrate: poly(L-Pro)


organism: Streptomyces griseoviridis


organism: Volvox carteri

substrate_name: poly(L-proline)

substrate: poly(L-proline)


enzyme_display_name: tyrosinase

organism: Aspergillus nidulans

substrate_name: Hydroquinone monomethylether

proof_lines: range of estimated values for crude or purified extracts

enzyme: tyrosinase

substrate: Hydroquinone monomethylether


organism: Mus musculus


organism: Mytilus edulis


organism: Panagrellus silusiae

substrate_name: Protocollagen

substrate: Protocollagen


organism: Paramecium bursaria Chlorella virus 1

proof_lines:
recombinant enzyme
MW: 13000, recombinant enzyme
MW: 40000, recombinant enzyme


organism: Homo sapiens

assay_conditions_summary: pH 6.8; 298.15 K; buffer 50 mM HEPES

proof_lines: mutant enzyme D543E, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: mutant enzyme V379S, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: mutant enzyme E304R, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: mutant enzyme V518T, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: mutant enzyme E376D, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


enzyme_display_name: formate dehydrogenase

organism: Glycine max

substrate_name: NAD+

assay_conditions_summary: pH 7; buffer 0.1 M sodium phosphate; cofactor NAD+

proof_lines: mutant enzyme F290A, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication

enzyme: formate dehydrogenase

substrate: NAD+


proof_lines: mutant enzyme V379S/V380T, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: mutant enzyme V380T, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: wild type enzyme, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: mutant enzyme T282S, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: mutant enzyme S516N, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


proof_lines: mutant enzyme F391Y, in 50 mM HEPES buffer (pH 6.8) containing 200 mM NaCl, and 0.01 mM ZnCl2, at 25°C


organism: Oryctolagus cuniculus

proof_lines: pH 7.5, temperature not specified in the publication

proof_lines: pH and temperature not specified in the publication


enzyme_display_name: alcohol dehydrogenase (NADP+)

organism: Moorella sp. HUC22-1

substrate_name: acetaldehyde

proof_lines:
isoform AdhA, pH 8.0, 60°C
isoform AdhB, pH 8.0, 60°C

enzyme: alcohol dehydrogenase (NADP+)

substrate: acetaldehyde


organism: Ovis aries

substrate_name: NADPH

assay_conditions_summary: cofactor NADPH

proof_lines:
p-nitrobenzaldehyde as substrate
D-glucuronate as substrate
pyridine 3-aldehyde as substrate

substrate: NADPH


organism: Euglena gracilis

substrate_name: NADP+

assay_conditions_summary: pH 8.8; 303.15 K; cofactor NADP+

substrate: NADP+


organism: Gallus sp.

assay_conditions_summary: pH 6; 298.15 K; cofactor NADPH


substrate_name: phenylethanediol

proof_lines:
aldehyde reductase I
aldehyde reductase II
AR I

substrate: phenylethanediol


organism: Bos taurus

substrate_name: menadione

substrate: menadione


organism: Canis lupus familiaris

substrate_name: D-glucuronate

assay_conditions_summary: pH 6.2; 298.15 K; cofactor NADPH

proof_lines:
outer medulla enzyme
inner medulla enzyme
cortex enzyme

substrate: D-glucuronate


organism: Cricetinae

substrate_name: Succinic semialdehyde

substrate: Succinic semialdehyde


organism: Escherichia coli

substrate_name: butanaldehyde

assay_conditions_summary: pH 7; 310.15 K; cofactor NADPH

substrate: butanaldehyde


enzyme_display_name: catechol oxidase

organism: Streptomyces lavendulae

substrate_name: Catechol; O2

kcat_over_km_origin: calculated

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

km_unit: mM

data_origin: experimental

enzyme: catechol oxidase

substrate: Catechol; O2


organism: Pelophylax perezi

substrate_name: Butanal

assay_conditions_summary: pH 10

proof_lines:
ethanol as substrate
m-nitrobenzaldehyde as substrate
at pH 10

substrate: Butanal


organism: Phycomyces blakesleeanus

substrate_name: n-butanol

assay_conditions_summary: cofactor NADP+

substrate: n-butanol


organism: Platyrrhini


organism: Pseudothermotoga hypogea


organism: Agroathelia rolfsii

substrate_name: cellobiose

proof_lines: electron acceptors methylene blue or methylene green

proof_lines: electron acceptor cytochrome c from horseheart

substrate: cellobiose


organism: Arabidopsis thaliana

substrate_name: cellotetraose

substrate: cellotetraose


organism: Thermococcus paralvinellae

assay_conditions_summary: pH 8; 353.15 K; cofactor NADPH


organism: Thermococcus sp.

substrate_name: butanol

substrate: butanol


organism: Trypanosoma cruzi

substrate_name: n-Propanol

proof_lines:
benzaldehyde as substrate
benzyl alcohol as substrate
n-propanol as substrate

substrate: n-Propanol


organism: Vigna radiata

substrate_name: coniferylaldehyde

substrate: coniferylaldehyde


organism: Pyrococcus furiosus

substrate_name: methylglyoxal

proof_lines: 80°C, pH not specified in the publication

substrate: methylglyoxal


organism: Rattus norvegicus

substrate_name: hexadecanal

proof_lines:
enzyme from liver and kidney
enzyme from kidney, brain or liver
enzyme from brain or liver

substrate: hexadecanal


organism: Saccharomyces cerevisiae

substrate_name: cinnamyl alcohol

assay_conditions_summary: pH 10; 298.15 K; cofactor NADPH

proof_lines:
pH 10.0, 25°C
pH 7.0, 25°C
coenzyme NADPH

substrate: cinnamyl alcohol


organism: Solanum tuberosum


organism: Coniophora puteana (Schum ex Fr) Karsten

substrate_name: Unknown

substrate: Unknown


organism: Coprinopsis cinerea


organism: Fungi

substrate_name: cytochrome c

substrate: cytochrome c


organism: Hypoxylon haematostroma

substrate_name: D-glucose

substrate: D-glucose


organism: Monilia sitophila


organism: Mycothermus thermophilus

substrate_name: xylobiose

substrate: xylobiose


organism: Neurospora crassa

proof_lines: recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C


organism: Sporobolomyces salmonicolor

substrate_name: octyl 4-chloro-3-oxobutanoate

substrate: octyl 4-chloro-3-oxobutanoate


organism: Sus scrofa

proof_lines:
reductase I
low-Km aldehyde reductase
high-Km aldehyde reductase


organism: Synechocystis sp.


organism: Thermoanaerobacter brockii


organism: Thermoanaerobacter ethanolicus

substrate_name: ethanol

proof_lines:
2° Adh
1°Adh, acetaldehyde as substrate
2° Adh, butan-2-ol as substrate

substrate: ethanol


organism: Thermococcus hydrothermalis

substrate_name: benzyl alcohol

proof_lines:
co-substrate NADP
benzyl aclohol as substrate
benzaldehyde as substrate

substrate: benzyl alcohol


organism: Thermococcus litoralis

substrate_name: NADPH

substrate: NADPH


organism: Phanerochaete sordida

substrate_name: lactose

substrate: lactose


organism: Phanerodontia chrysosporium

substrate_name: cellobiose

proof_lines: pH 4.5, 23°C, recombinant wild-type enzyme

substrate: cellobiose


substrate_name: cellulose

proof_lines:
ferricyanide as electron acceptor
pH 4.5, 22°C
pH 4.0, 30°C, recombinant enzyme

substrate: cellulose


organism: Podospora anserina

substrate_name: cellotriose

substrate: cellotriose


enzyme_display_name: monoamine oxidase

organism: Avena sativa

enzyme: monoamine oxidase


organism: Bos taurus

proof_lines:
pH 7.2, 30°C
MAO-B


organism: Coregonus lavaretus ludoga

substrate_name: serotonin

assay_conditions_summary: pH 7.4; 296.15 K; buffer 0.01 M phosphate

proof_lines: in 0.01 M phosphate buffer, pH 7.4, at 23°C

substrate: serotonin


organism: Danio rerio

proof_lines: at 25°C in 50 mM potassium phosphate, pH 7.4


organism: Thermothelomyces fergusii

proof_lines: recombinant enzyme from Trichoderma reesei, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C

proof_lines: recombinant enzyme from Escherichia coli, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C


organism: Thermothelomyces heterothallicus

proof_lines:
at 45°C
at 25°C
at pH 4.5 and 45°C


organism: Thermothelomyces myriococcoides

substrate_name: ferricytochrome c

assay_conditions_summary: pH 5.5; 303.15 K; buffer 100 mM sodium acetate

proof_lines: in the presence of 30 mM NaCl, at pH 5.5 and 30°C

proof_lines: in the presence of 30 mM BaCl2, at pH 5.5 and 30°C

proof_lines: in the presence of 30 mM CaCl2, at pH 7.5 and 30°C

substrate: ferricytochrome c


organism: Trametes cinnabarina


organism: Equus caballus


organism: Homo sapiens

substrate_name: benzylamine

proof_lines:
recombinant MAO-B
MAO-A
11°C

substrate: benzylamine


organism: Huso huso


organism: Katsuwonus pelamis


organism: Acipenser gueldenstaedtii

substrate_name: N-methylhistamine

substrate: N-methylhistamine


organism: Acipenser persicus


organism: Acipenser stellatus


organism: Trametes hirsuta

proof_lines:
at pH 4.5
electron donor
electron acceptor


organism: Trametes pubescens

substrate_name: cellotetraose

proof_lines: pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol

substrate: cellotetraose


organism: Trametes versicolor


organism: Trametes villosa


organism: Acidithiobacillus caldus

substrate_name: UDP

assay_conditions_summary: pH 7; 333.15 K; cofactor ADP

substrate: UDP


organism: Anabaena sp.

substrate_name: UDP-alpha-D-glucose

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ADP

proof_lines: with ADP-alpha-D-glucose as cosubstrate, at pH 7.5 and 30°C

substrate: UDP-alpha-D-glucose


organism: Arabidopsis thaliana

assay_conditions_summary: pH 6; 298.15 K; cofactor ADP

proof_lines: isoform SUS1, with UDP as cosubstrate, at pH 6.0 and 25°C


organism: Beta vulgaris

proof_lines: isoform SUS1, with UDP as cosubstrate, at pH 6.5 and 35°C


organism: Ipomoea batatas

substrate_name: ADP

assay_conditions_summary: pH 6; 310.15 K; cofactor ADP

proof_lines: with UDP-alpha-D-glucose as cosubstrate, at pH 7.5 and 37°C

substrate: ADP


organism: Lathyrus oleraceus

proof_lines: isoform SUS3, with UDP-alpha-D-glucose as cosubstrate, at pH 9.0 and 30°C


organism: Manihot esculenta

substrate_name: D-fructose

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ADP

proof_lines: with ADP as cosubstrate, at pH 7.5 and 37°C

substrate: D-fructose


organism: Nicotiana tabacum

substrate_name: sucrose

proof_lines:
SuSy2, 25°C
SuSy1, 25°C
25°C, isoform SuSy2

substrate: sucrose


organism: Schizophyllum commune


organism: Stachybotrys bisbyi

substrate_name: D-glucose

substrate: D-glucose


organism: Daucus carota


organism: Glycine max

substrate_name: UDPglucose

assay_conditions_summary: cofactor ADP

proof_lines:
cosubstrate CDP
cosubstrate ADPglucose

substrate: UDPglucose


organism: Helianthus tuberosus

proof_lines: with UDP-alpha-D-glucose as cosubstrate, at pH 8.0 and 30°C

proof_lines: with UDP as cosubstrate, at pH 8.3 and 30°C


organism: Hordeum vulgare

assay_conditions_summary: pH 7; 310.15 K; cofactor ADP

proof_lines: with ADP as cosubstrate, at pH 7.0 and 37°C

proof_lines: with UDP as cosubstrate, at pH 7.0 and 37°C


organism: Macaca fuscata

substrate_name: Unknown

substrate: Unknown


organism: Mesocricetus auratus

substrate_name: Phenylethylamine

substrate: Phenylethylamine


organism: Mus musculus


organism: Narcissus tazetta


organism: Oncorhynchus keta


organism: Papio anubis

proof_lines: pH not specified in the publication, temperature not specified in the publication, MAO-B


organism: Papio hamadryas

proof_lines: with 1-methyl-4-(1-methylpyrrol-2-yl)1,2,3,6-tetrahydropyridine as substrate


organism: Papio sp.


organism: Rattus norvegicus

assay_conditions_summary: pH 9; 298.15 K; buffer 50 mM potassium phosphate assay

proof_lines: isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C


organism: Sus scrofa


organism: Todarodes pacificus

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Zea mays

assay_conditions_summary: pH 6.5; 303.15 K; cofactor ADP

proof_lines:
cosubstrate UDPglucose, ADP
at pH 6.5 and 30°C
at pH 8.0 and 30°C


organism: Nitrosomonas europaea

substrate_name: ADP-alpha-D-glucose

assay_conditions_summary: pH 8; 310.15 K; cofactor ADP

proof_lines: with ADP as cosubstrate, at pH 7.0 and 60°C

proof_lines: with ADP-alpha-D-glucose as cosubstrate, at pH 8.0 and 37°C

substrate: ADP-alpha-D-glucose


organism: Oryza sativa

proof_lines: with UDP-alpha-D-glucose as cosubstrate, at pH 8.0 and 37°C


organism: Populus tremuloides


organism: Prunus persica

proof_lines: with UDP-alpha-D-glucose as cosubstrate, at pH 8.5 and 25°C


organism: Pyrus pyrifolia

assay_conditions_summary: pH 7; 303.15 K; cofactor ADP

proof_lines: isoform SUS2, with UDP as cosubstrate, at pH 7.0 and 30°C

proof_lines: isoform SUS2, with UDP-alpha-D-glucose as cosubstrate, at pH 8.0 and 30°C


organism: Ricinus communis

proof_lines: in the presence of 0.2 mM trehalose 6-phosphate, at pH 7.0 and 25°C

proof_lines: in the absence of trehalose 6-phosphate, at pH 7.0 and 25°C


organism: Saccharum officinarum

assay_conditions_summary: pH 7

proof_lines:
pH 7.0, isoform SuSyC
pH 7.5, isoform SuSyC
pH 7.5, isoform SuSyB


organism: Saccharum sp.

proof_lines: at pH 7.0, temperature not specified in the publication

proof_lines: at pH 7.3, temperature not specified in the publication


enzyme_display_name: anthranilate phosphoribosyltransferase

organism: Fructobacillus fructosus

substrate_name: 5-phospho-alpha-D-ribose 1-diphosphate

enzyme: anthranilate phosphoribosyltransferase

substrate: 5-phospho-alpha-D-ribose 1-diphosphate


organism: Methanocella conradii


organism: Methanococcus voltae


organism: Ogataea henricii

substrate_name: anthranilate

substrate: anthranilate


organism: Solanum chmielewskii

proof_lines: with UDP as cosubstrate, at pH 6.5 and 25°C


organism: Solanum tuberosum

proof_lines: isoform SUS1, with UDP-alpha-D-glucose as cosubstrate, at pH 9.0 and 30°C


organism: Tetradesmus obliquus


organism: Thermosynechococcus vestitus

proof_lines: with ADP-alpha-D-glucose as cosubstrate, at pH 7.0 and 37°C

proof_lines: pH 7.0, 37°C, purified recombinant enzyme, with ADP in sucrose cleavage


organism: Acetomicrobium mobile


organism: Citrus sinensis


organism: Coprococcus eutactus


organism: Escherichia coli

proof_lines:
complex
component II


organism: Triticum aestivum

proof_lines:
at pH 6.5 and 37 °C
cosubstrate UDP


organism: Vicia faba

assay_conditions_summary: pH 7; 298.15 K; buffer cosubstrate UDP; cofactor ADP

proof_lines: with UDP as cosubstrate, at pH 7.0 and 25°C


organism: Vigna radiata

assay_conditions_summary: pH 7.5; 299.15 K; cofactor ADP

proof_lines: with UDP-alpha-D-glucose as cosubstrate, at pH 7.5, temperature not specified in the publication


organism: Vigna radiata var. radiata

substrate_name: GDP

proof_lines:
cosubstrate ADP
cosubstrate TDP

substrate: GDP


organism: Staphylococcus aureus


organism: Staphylococcus haemolyticus


organism: Artemia sp.

substrate_name: guanine

substrate: guanine


organism: Bos taurus


organism: Caldanaerobacter subterraneus subsp. tengcongensis

proof_lines: pH 7.4, 12 mM MgCl2, in presence of 0.05 mM IMP

proof_lines: pH 7.4, 12 mM MgCl2, versus 5-phospho-alpha-D-ribose 1-diphosphate


organism: Cricetulus griseus

substrate_name: hypoxanthine

substrate: hypoxanthine


organism: Pelodictyon luteolum


organism: Petrotoga mobilis


organism: Physcomitrium patens

proof_lines: Ki above 5 mM, wild type enzyme, at pH 7.4 and 30°C


organism: Pistacia vera


proof_lines:
at pH 7.4 and 25°C
Ki above 0.01 mM, at pH 7.4 and 25°C


organism: Gallus gallus


organism: Giardia duodenalis

proof_lines:
wild-type enzyme
wild-type enzyme, reverse reaction
wild-type, forward reaction


organism: Helicobacter pylori

proof_lines: pH and temperature not specified in the publication


organism: Homo sapiens


substrate_name: inosine monophosphate

proof_lines: purified recombinant chimeric enzyme DS1

substrate: inosine monophosphate


organism: Methanococcus voltae


organism: Saccharolobus solfataricus

proof_lines:
at 87°C
at 60°C
at 25°C


organism: Saccharomyces cerevisiae


organism: Salmonella enterica subsp. enterica serovar Typhimurium


organism: Selaginella moellendorffii


organism: Plasmodium vivax


organism: Pyrococcus horikoshii

substrate_name: xanthine

substrate: xanthine


organism: Schistosoma mansoni

proof_lines:
recombinant enzyme
native enzyme


organism: Mycobacterium tuberculosis

proof_lines: pH 7.4, 25°C, recombinant enzyme, with hypoxanthine

proof_lines: pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate

proof_lines: pH 7.4, 25°C, recombinant enzyme, with guanine


organism: Plasmodium chabaudi


organism: Plasmodium falciparum

proof_lines: at pH 7.4, temperature not specified in the publication

proof_lines: recombinant enzyme, with 5-phosphoribosyl 1-diphosphate, pH 8.5


organism: Plasmodium lophurae


organism: Schizosaccharomyces pombe


organism: Streptomyces cyanogenus


organism: Tritrichomonas suis


proof_lines: mutant K134S, competitive versus 5-phospho-alpha-D-ribose 1-diphosphate


organism: Trypanosoma cruzi

mutation_signature: loop II-deletion mutant


substrate_name: diphosphate

proof_lines: pH 7.5, 37°C, recombinant enzyme, with GMP

proof_lines: pH 7.5, 37°C, recombinant enzyme, with IMP

substrate: diphosphate


enzyme_display_name: glutathione transferase

organism: Hevea brasiliensis

enzyme: glutathione transferase


organism: Schistosoma japonicum

proof_lines: 25°C, 20 mM sodium phosphate buffer, pH 7.0, 0.5 mM glutathione

proof_lines: 4°C, 20 mM sodium phosphate buffer, pH 6.5, 0.5 mM glutathione


enzyme_display_name: creatine kinase

organism: Homo sapiens

substrate_name: ATP

assay_conditions_summary: cofactor ATP

proof_lines:
mutant D54G
mutant enzyme D54G

enzyme: creatine kinase

substrate: ATP


substrate_name: Creatine

assay_conditions_summary: pH 8; 298.15 K; cofactor ATP

proof_lines: co-substrate: ATP, wild-type hMMCK, pH and temperature not specified in the publication

proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, wild-type

substrate: Creatine


assay_conditions_summary: 298.15 K; cofactor ATP

proof_lines: co-substrate: ATP, 25°C, pH not specified in the publication, mutant K304T


organism: Lethenteron camtschaticum

proof_lines:
cytoplasmic isoform M2, 25°C
cytoplasmic isoform M1, 25°C


organism: Mus musculus

assay_conditions_summary: pH 6.5; 298.15 K; cofactor ATP

proof_lines:
cytoplasmic isoform M, 25°C
cytoplasmic isoform B, 25°C
mitochondrial isoform, 25°C


organism: Pagrus major

substrate_name: ADP

substrate: ADP


organism: Pelodiscus sinensis

assay_conditions_summary: pH 9; 293.15 K; cofactor ATP

proof_lines:
pH 9.0, 20°C
versus ATP, pH 9.0, 20°C
versus creatine, pH 9.0, 20°C


organism: Physeter macrocephalus

proof_lines:
muscle-type isoform MCK
mitochondrial isoform sMiCK


organism: Aeromonas caviae

proof_lines: in presence of 0.5 mM fructose 6-phosphate


organism: Aeromonas hydrophila

assay_conditions_summary: pH 8; 310.15 K; cofactor ATP

proof_lines: pH 8.0, 37°C, in presence of fructose 6-phosphate

proof_lines: pH 8.0, 37°C, in presence of fructose 1,6-bisphosphate


organism: Agrobacterium tumefaciens

substrate_name: alpha-D-glucose 1-phosphate

proof_lines: pH 8.0, 37°C, wild-type enzyme in presence of 0.3 mM fructose-1,6-bisphosphate

substrate: alpha-D-glucose 1-phosphate


organism: Anabaena sp.

assay_conditions_summary: pH 7.5; 278.15 K; cofactor ATP

proof_lines:
pH 7.5, 5°C, kinetic study
pH 7.0, 37°C, kinetic study
pH 7.5, 30°C, kinetic study


organism: Rattus norvegicus

substrate_name: creatine phosphate

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP

proof_lines: 0.005 mM organotellurium, preincubation time 5 min, mitochondrial fraction, pH 7.5, 37°C

substrate: creatine phosphate


organism: Torpedo marmorata

assay_conditions_summary: cofactor ADP

proof_lines: acetylcholine receptor membrane-asscociated enzyme


organism: Urocitellus richardsonii

proof_lines:
25°C, myosin bound enzyme
25°C, hibernating squirrel
25°C, euthermic squirrel


organism: Dendronephthya gigantea

proof_lines:
mitochondrial isoform, 25°C
mitochondrial isoform M2, 25°C


organism: Gallus gallus


organism: Ginglymostoma cirratum


organism: Hediste diversicolor

proof_lines:
mitochondrial isoform, 25°C
cytoplasmic isoform, 25°C
cytoplasmic isoform M1, 25°C


organism: Bos taurus

assay_conditions_summary: pH 7.4; 303.15 K; cofactor ATP

proof_lines:
30°C
ADP in form of MgADP-
pH 7.4, dimeric form


organism: Chaenocephalus aceratus

assay_conditions_summary: pH 7.6; 273.65 K; cofactor ADP


organism: Cryptosporidium muris

proof_lines: pH and temperature not specified in the publication


organism: Danio rerio

proof_lines:
cytoplasmic isoform M2, 25°C
cytoplasmic isoform M1, 25°C
cytoplasmic isoform B, 25°C


organism: Auxenochlorella pyrenoidosa

assay_conditions_summary: pH 8.5; cofactor ADP

proof_lines: pH 8.5, in presence of 2 mM 3-phosphoglycerate

proof_lines: pH 8.5, in absence of 3-phosphoglycerate


organism: Cereibacter sphaeroides

proof_lines: in absence of 0.1 mM fructose 6-phosphate

proof_lines: in absence of 0.01 mM fructose 6-phosphate


organism: Chlamydomonas reinhardtii

proof_lines: pH 8.0, 37°C, in presence of 2.5 mM 3-phosphoglycerate

proof_lines: pH 8.0, 37°C, in absence of 3-phosphoglycerate


organism: Citrobacter freundii

assay_conditions_summary: pH 8.4; 310.15 K; cofactor ATP

proof_lines: pH 8.4, 37°C, influence of activators on Km


organism: Escherichia coli

assay_conditions_summary: pH 7.6; 310.15 K; cofactor ADP


assay_conditions_summary: pH 7; 310.15 K; cofactor ATP

proof_lines: pH 7.0, 37°C, strain AC70R1, kinetic study

proof_lines: S0.5 value, Hill coefficient 1.0, presence of 25 mM pyruvate and 0.01 mM fructose 1,6-bisphosphate, pH 8.0, 37°C


organism: Hafnia alvei

proof_lines: pH 8.0, 37°C, in absence of phosphoenolpyruvate

proof_lines: pH 8.0, 37°C, in presence of phosphoenolpyruvate


organism: Hordeum vulgare

assay_conditions_summary: pH 8; cofactor ATP

proof_lines: pH 8.0, in presence of 2.5 mM 3-phosphoglycerate

proof_lines: pH 8.0, in absence of 3-phosphoglycerate


enzyme_display_name: flavivirin

organism: West Nile virus

assay_conditions_summary: pH 8; 295.15 K; buffer 20 mM Tris/HCl

proof_lines: mutant enzyme R76L, in 20 mM Tris/HCl buffer, pH 8.0, at 22°C

enzyme: flavivirin


proof_lines: mutant enzyme T52V, in 20 mM Tris/HCl buffer, pH 8.0, at 22°C


organism: Solanum tuberosum

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

proof_lines: the phosphate inhibition pattern of Mos(1-198) is complex and biphasic. Inhibition at relatively high phopshate concentrations is less than predicted at low phosphate concentrations, the calculated Ki for phosphate is about 1 mM before 50%

proof_lines: chimeric mutant, substrate ATP, pH 7.4, 37°C

proof_lines: chimeric mutant, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C


proof_lines: chimeric enzyme, pH 7.4, 37°C, presence of 3-phosphoglycerate

proof_lines: chimeric enzyme, pH 7.4, 37°C, presence of phosphate


organism: Spinacia oleracea

assay_conditions_summary: pH 7.3; 310.15 K; cofactor ATP

proof_lines: pH 7.3, 37°C, in presence of 1 mM 3-phosphoglycerate

proof_lines: pH 7.3, 37°C, in absence of 3-phosphoglycerate

proof_lines: pH 7.5, in presence of 1 mM 3-phosphoglycerate


organism: Synechococcus sp.


enzyme_display_name: carboxylesterase

organism: uncultured bacterium

substrate_name: 4-nitrophenyl butyrate

proof_lines: recombinant wild-type enzyme, pH 8.5, 30°C

enzyme: carboxylesterase

substrate: 4-nitrophenyl butyrate


enzyme_display_name: acetylcholinesterase

organism: Anopheles gambiae

substrate_name: Acetylcholine

enzyme: acetylcholinesterase

substrate: Acetylcholine


organism: Apis mellifera

substrate_name: acetylthiocholine

proof_lines: isoform AChE2, at 30°C, pH not specified in the publication

proof_lines: isoform AChE1, at 30°C, pH not specified in the publication

substrate: acetylthiocholine


organism: Arapaima gigas

assay_conditions_summary: pH 8; buffer 0.5 M Tris-HCl

proof_lines: in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication


organism: Bactrocera dorsalis

substrate_name: propionylthiocholine

proof_lines:
25°C
enzyme from larva, at pH 7.4 and 37°C
enzyme from pupa, at pH 7.4 and 37°C

substrate: propionylthiocholine


organism: Lens culinaris

proof_lines:
pH 7.4, 37°C, long day photoperiod
pH 7.4, 37°C, short day photoperiod


organism: Mycolicibacterium smegmatis


organism: Oryza sativa

proof_lines: S0.5 value, large subunit mutant T139I with wild-type small subunit, 37°C, pH 7.0

proof_lines: S0.5 value, large subunit mutant A171V with wild-type small subunit, 37°C, pH 7.0

proof_lines: S0.5 value, wild-type small subunit alone, 37°C, pH 7.0


proof_lines: pH 8.0, in presence of 5 mM 3-phosphoglycerate

proof_lines: pH 8.0, in presence of 1 mM 3-phosphoglycerate

proof_lines: pH 8.0, in presence of 0.5 mM 3-phosphoglycerate


organism: Rhodobacter capsulatus

substrate_name: Unknown

substrate: Unknown


organism: Rhodocyclus tenuis

proof_lines:
in presence of 0.05 mM pyruvate
in absence of pyruvate
in presence of 1 mM pyruvate


organism: Salmonella enterica subsp. enterica serovar Typhimurium

proof_lines: pH 8.0, 37°C, strain JP102, kinetic study

proof_lines: pH 8.4, 37°C, influence of activators on Km

proof_lines: pH 8.0, 37°C, strain LT-2, kinetic study


organism: Serratia liquefaciens


organism: Eledone moschata

proof_lines:
form II
form I


organism: Gallotia galloti

substrate_name: acetylcholine iodide

substrate: acetylcholine iodide


organism: Gambusia yucatana

proof_lines: enzyme from head, pH 7.4, temperature not specified in the publication

proof_lines: enzyme from muscle, pH 7.4, temperature not specified in the publication


organism: Gammarus pulex


organism: Synechocystis sp.

proof_lines: pH 7.0, 37°C, in presence of 2.5 mM 3-phosphoglycerate

proof_lines: pH 7.0, 37°C, in absence of 3-phosphoglycerate


organism: Thermus caldophilus

assay_conditions_summary: pH 7.6; 348.15 K; cofactor ATP


organism: Zea mays

proof_lines: pH 7.4, 37°C, enzyme from Zea mays endosperm


proof_lines:
pH 7.4, 37°C, mutant BT2-TI
pH 7.4, 37°C, mutant MP
pH 7.4, 37°C, mutant MP-TI


organism: Blattella germanica

substrate_name: acetylthiocholine iodide

proof_lines: recombinant enzyme AChE1, pH not specified in the publication, 30°C

proof_lines: recombinant enzyme AChE2, pH not specified in the publication, 30°C

substrate: acetylthiocholine iodide


organism: Bos taurus


organism: Carassius auratus auratus


organism: Cavia porcellus


organism: Colossoma macropomum

assay_conditions_summary: pH 8; 295.15 K; buffer 0.5 M Tris-HCl

proof_lines: in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication

proof_lines: in 0.5 mM Tris-HCl buffer, pH 7.4, at 22°C


organism: Dendrobaena veneta


organism: Diapterus auratus

proof_lines: pH 7.4, temperature not specified in the publication


organism: Diapterus rhombeus


organism: Diopatra neapolitana


organism: Drosophila melanogaster

proof_lines:
from eel
from human
from Drosophila


organism: Eisenia andrei

substrate_name: propionylthiocholine iodide

substrate: propionylthiocholine iodide


organism: Electrophorus electricus

proof_lines:
pH 7.4, 30°C, soluble free enzyme
pH 7.2
25°C, pH 8.0, untreated preparation


organism: Serratia marcescens

proof_lines:
pH 8.0, 37°C, strain ATCC 15365
pH 8.0, 37°C, strain ATCC 274


organism: Solanum lycopersicum

proof_lines: pH 7.4, 37°C, fruit enzyme, in presence of 3-phosphoglycerate

proof_lines: pH 7.4, 37°C, leaf enzyme, in presence of 3-phosphoglycerate


organism: Solanum tuberosum

proof_lines: mutation P52L, large subunit, plus mutation P308L, small subunit, 37°C, pH 7.0


proof_lines: reduced wild-type, pH 7.4, 37°C, presence of phosphate

proof_lines: oxidized wild-type, pH 7.4, 37°C, presence of phosphate


organism: Centropomus undecimalis


organism: Chelidonichthys lucerna

proof_lines: enzyme isolated from muscle, 25°C, pH not specified in the publication


organism: Cichla ocellaris


organism: Cimex lectularius


organism: Mytilus galloprovincialis

substrate_name: butyrylthiocholine

substrate: butyrylthiocholine


organism: Nephotettix cincticeps Uhler

proof_lines: pH 7.5, 25°C, N-methyl carbamate-susceptible strain

proof_lines: pH 7.5, 25°C, N-methyl carbamate-resistant strain

proof_lines: pH 7.5, 25°C, N-methyl carbamate-resisitant strain


organism: Nippostrongylus brasiliensis


organism: Oncorhynchus mykiss


organism: Magallana hongkongensis

proof_lines: in 80 mM sodium phosphate (pH 7.4), at 37°C


organism: Mus musculus


proof_lines: pH 7.2, 25°C, presence of aflatoxin B1 at 0.001 mM

proof_lines: pH 7.2, 25°C, presence of aflatoxin B1 at 0.1 mM


organism: Mytella charruana

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Homo sapiens

substrate_name: acetyl thiocholine

assay_conditions_summary: pH 8; 300.15 K; buffer 0.1 M Tris

proof_lines: pH 8, 27°C, attachment of polyethylene glycol side chains to lysine residues does not alter the Kcat value

proof_lines: pH 8, 27°C, attachment of polyethylene glycol side chains to lysine residues does not alter the Km value

substrate: acetyl thiocholine


organism: Hoplosternum littorale


organism: Lathyrus oleraceus


organism: Liposcelis entomophila

assay_conditions_summary: pH 7; 310.15 K; buffer 0.1 M phosphate

proof_lines: enzyme from Sichuan Province population, in 0.1 M phosphate buffer (pH 7.0), at 37°C

proof_lines: enzyme from Hubei Province population, in 0.1 M phosphate buffer (pH 7.0), at 37°C

proof_lines: enzyme from Chongqing Municipality population, in 0.1 M phosphate buffer (pH 7.0), at 37°C


organism: Locusta migratoria manilensis

assay_conditions_summary: pH 7.4; 297.15 K; buffer 100 mM phosphate

proof_lines: in 100 mM phosphate buffer, pH 7.4, at 30°C


organism: Lytechinus variegatus


organism: Macroptilium atropurpureum

substrate_name: propionylthiocholine chloride

substrate: propionylthiocholine chloride


organism: Oreochromis mossambicus

proof_lines:
tetrameric enzyme, pH 8, 30°C
tetrameric enzyme, pH 8, 10°C
tetrameric enzyme, pH 8, 20°C


organism: Oreochromis niloticus

proof_lines: in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication


organism: Oryctolagus cuniculus


organism: Ovis aries

proof_lines:
pH 7.4
pH 7.4, presence of cardiotoxin III
pH 7.4, presence of cardiotoxin IV


organism: Parachromis managuensis


organism: Perforatus perforatus


organism: Pollicipes pollicipes


organism: Pseudorasbora parva


organism: Sparus aurata

proof_lines:
pH 7.4, 25°C, enzyme from muscle
pH 7.4, 25°C, enzyme from brain


organism: Tetronarce californica


organism: Zea mays

proof_lines: pH 7.0, 30°C, substrate acetylthiocholine

proof_lines: pH 7.0, 30°C, substrate propionylthiocholine


organism: Sardina pilchardus

proof_lines: enzyme isolated from muscle, 25°C, pH not specified in the publication

proof_lines: enzyme isolated from brain, 25°C, pH not specified in the publication


organism: Schizaphis graminum

proof_lines: pH and temperature not specified in the publication


organism: Scomber scombrus


organism: Sitobion avenae

substrate_name: propylthiocholine

substrate: propylthiocholine


organism: Aspergillus terreus

substrate_name: methyl 4-coumarate

substrate: methyl 4-coumarate


organism: Aspergillus usamii

substrate_name: methyl ferulate

substrate: methyl ferulate


organism: Aureobasidium pullulans

substrate_name: methyl sinapate

substrate: methyl sinapate


organism: Auricularia auricula-judae


organism: Acetivibrio thermocellus

substrate_name: ethyl ferulate

substrate: ethyl ferulate


organism: Actinomyces sp.

substrate_name: methyl caffeate

substrate: methyl caffeate


organism: Alternaria tenuissima