organism: Cyberlindnera jadinii

substrate_name: uric acid

substrate: uric acid


enzyme_display_name: factor independent urate hydroxylase

organism: Lysobacter sp. T-15

substrate_name: Urate; O2; H2O

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

km_unit: mM

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

enzyme: factor independent urate hydroxylase

substrate: Urate; O2; H2O


organism: Phaseolus vulgaris

source_db: brenda

source_license: CC BY 4.0

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

source: brenda


organism: Neurospora crassa


organism: Vicia faba


organism: Puccinia recondita


organism: Streptomyces cyanogenus


enzyme_display_name: ferredoxin--nitrite reductase

organism: Cucurbita pepo

substrate_name: nitrite

km_display: Not reported

enzyme: ferredoxin--nitrite reductase

substrate: nitrite


organism: Vigna unguiculata


organism: Papio sp.

substrate_name: Urate

substrate: Urate


organism: Haloferax mediterranei

substrate_name: reduced methyl viologen

substrate: reduced methyl viologen


organism: Nicotiana tabacum

source_db: oed

mutation_type: substitution

source: oed


organism: Leptolyngbya laminosa


organism: Rattus norvegicus

substrate_name: trans-Benzene dihydrodiol

verification_status: verified

protein_accession_database: UniProt

source_license: as published (see paper)

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NAD+

column_label: Table 1, Oxidation section, substrate row 'trans-Benzene dihydrodiol'; TBER1 column 'Km (uM)' = 215; TBER1 column 'kcat (min^-1)' = 38; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.18.

normalized_value_unit_evidence: Km: printed cell 215 uM; 215 / 1000 = 0.215 mM; the exported value is 0.215 mM. kcat: printed cell 38 min^-1; the printed turnover column is per MINUTE, so 38 / 60 = 0.6333333333333333 s^(-1); the exported value is 0.6333333333 s^(-1). kcat/Km: printed cell 0.18 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.18 x 1000 / 60 = 3.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Oxidation row 'trans-Benzene dihydrodiol', TBER1 column group: Km = 215 uM; kcat = 38 min^-1; printed kcat/Km = 0.18 min^-1 uM^-1.

row_label: Table 1, Oxidation section, substrate row 'trans-Benzene dihydrodiol', TBER1 column group

table_label: Table 1: Substrate specificity for non-steroidal substrates in the oxidation and reduction reactions catalyzed by TBER1 and rat 17HSD5 (J. Biochem. 2006, 139(6):1053-1063, journal page 1058)

substrate: trans-Benzene dihydrodiol

status: verified


column_label: Table 1, Oxidation section, substrate row '2-Cyclohexen-1-ol'; TBER1 column 'Km (uM)' = 1500; TBER1 column 'kcat (min^-1)' = 26; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.02.

normalized_value_unit_evidence: Km: printed cell 1500 uM; 1500 / 1000 = 1.5 mM; the exported value is 1.5 mM. kcat: printed cell 26 min^-1; the printed turnover column is per MINUTE, so 26 / 60 = 0.43333333333333335 s^(-1); the exported value is 0.4333333333 s^(-1). kcat/Km: printed cell 0.02 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.02 x 1000 / 60 = 0.3333333333333333 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Oxidation row '2-Cyclohexen-1-ol', TBER1 column group: Km = 1500 uM; kcat = 26 min^-1; printed kcat/Km = 0.02 min^-1 uM^-1.

row_label: Table 1, Oxidation section, substrate row '2-Cyclohexen-1-ol', TBER1 column group


enzyme_display_name: 17beta-estradiol 17-dehydrogenase

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADP+

column_label: Table 1, Oxidation section, substrate row '2-Cyclohexen-1-ol'; Rat 17HSD5 column 'Km (uM)' = 7000; Rat 17HSD5 column 'kcat (min^-1)' = 3.5; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.0005.

normalized_value_unit_evidence: Km: printed cell 7000 uM; 7000 / 1000 = 7.0 mM; the exported value is 7.0 mM. kcat: printed cell 3.5 min^-1; the printed turnover column is per MINUTE, so 3.5 / 60 = 0.058333333333333334 s^(-1); the exported value is 0.0583333333 s^(-1). kcat/Km: printed cell 0.0005 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.0005 x 1000 / 60 = 0.008333333333333333 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Oxidation row '2-Cyclohexen-1-ol', Rat 17HSD5 column group: Km = 7000 uM; kcat = 3.5 min^-1; printed kcat/Km = 0.0005 min^-1 uM^-1.

row_label: Table 1, Oxidation section, substrate row '2-Cyclohexen-1-ol', Rat 17HSD5 column group

enzyme: 17beta-estradiol 17-dehydrogenase


column_label: Table 1, Oxidation section, substrate row '9a,11a-PGF2'; TBER1 column 'Km (uM)' = 72; TBER1 column 'kcat (min^-1)' = 0.17; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.002.

normalized_value_unit_evidence: Km: printed cell 72 uM; 72 / 1000 = 0.072 mM; the exported value is 0.072 mM. kcat: printed cell 0.17 min^-1; the printed turnover column is per MINUTE, so 0.17 / 60 = 0.0028333333333333335 s^(-1); the exported value is 0.0028333333 s^(-1). kcat/Km: printed cell 0.002 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.002 x 1000 / 60 = 0.03333333333333333 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Oxidation row '9a,11a-PGF2', TBER1 column group: Km = 72 uM; kcat = 0.17 min^-1; printed kcat/Km = 0.002 min^-1 uM^-1.

row_label: Table 1, Oxidation section, substrate row '9a,11a-PGF2', TBER1 column group


column_label: Table 1, Oxidation section, substrate row '9a,11a-PGF2'; Rat 17HSD5 column 'Km (uM)' = 54; Rat 17HSD5 column 'kcat (min^-1)' = 0.51; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.01.

normalized_value_unit_evidence: Km: printed cell 54 uM; 54 / 1000 = 0.054 mM; the exported value is 0.054 mM. kcat: printed cell 0.51 min^-1; the printed turnover column is per MINUTE, so 0.51 / 60 = 0.0085 s^(-1); the exported value is 0.0085 s^(-1). kcat/Km: printed cell 0.01 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.01 x 1000 / 60 = 0.16666666666666666 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Oxidation row '9a,11a-PGF2', Rat 17HSD5 column group: Km = 54 uM; kcat = 0.51 min^-1; printed kcat/Km = 0.01 min^-1 uM^-1.

row_label: Table 1, Oxidation section, substrate row '9a,11a-PGF2', Rat 17HSD5 column group


column_label: Table 1, Reduction section, substrate row '9,10-Phenanthrenequinone'; TBER1 column 'Km (uM)' = 0.4; TBER1 column 'kcat (min^-1)' = 157; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 393.

normalized_value_unit_evidence: Km: printed cell 0.4 uM; 0.4 / 1000 = 0.0004 mM; the exported value is 0.0004 mM. kcat: printed cell 157 min^-1; the printed turnover column is per MINUTE, so 157 / 60 = 2.6166666666666667 s^(-1); the exported value is 2.6166666667 s^(-1). kcat/Km: printed cell 393 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 393 x 1000 / 60 = 6550.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row '9,10-Phenanthrenequinone', TBER1 column group: Km = 0.4 uM; kcat = 157 min^-1; printed kcat/Km = 393 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row '9,10-Phenanthrenequinone', TBER1 column group


substrate_name: TBE

smiles: C(C(Br)Br)(Br)Br

column_label: Table 1, Reduction section, substrate row 'TBE'; TBER1 column 'Km (uM)' = 180; TBER1 column 'kcat (min^-1)' = 75; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.42.

normalized_value_unit_evidence: Km: printed cell 180 uM; 180 / 1000 = 0.18 mM; the exported value is 0.18 mM. kcat: printed cell 75 min^-1; the printed turnover column is per MINUTE, so 75 / 60 = 1.25 s^(-1); the exported value is 1.25 s^(-1). kcat/Km: printed cell 0.42 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.42 x 1000 / 60 = 7.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row 'TBE', TBER1 column group: Km = 180 uM; kcat = 75 min^-1; printed kcat/Km = 0.42 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row 'TBE', TBER1 column group

substrate: TBE


column_label: Table 1, Reduction section, substrate row 'TBE'; Rat 17HSD5 column 'Km (uM)' = 16; Rat 17HSD5 column 'kcat (min^-1)' = 9.2; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.58.

normalized_value_unit_evidence: Km: printed cell 16 uM; 16 / 1000 = 0.016 mM; the exported value is 0.016 mM. kcat: printed cell 9.2 min^-1; the printed turnover column is per MINUTE, so 9.2 / 60 = 0.15333333333333332 s^(-1); the exported value is 0.1533333333 s^(-1). kcat/Km: printed cell 0.58 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.58 x 1000 / 60 = 9.666666666666666 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row 'TBE', Rat 17HSD5 column group: Km = 16 uM; kcat = 9.2 min^-1; printed kcat/Km = 0.58 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row 'TBE', Rat 17HSD5 column group


smiles: CCC(=O)C(=O)C

column_label: Table 1, Reduction section, substrate row '2,3-Pentanedione'; TBER1 column 'Km (uM)' = 385; TBER1 column 'kcat (min^-1)' = 29; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.08.

normalized_value_unit_evidence: Km: printed cell 385 uM; 385 / 1000 = 0.385 mM; the exported value is 0.385 mM. kcat: printed cell 29 min^-1; the printed turnover column is per MINUTE, so 29 / 60 = 0.48333333333333334 s^(-1); the exported value is 0.4833333333 s^(-1). kcat/Km: printed cell 0.08 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.08 x 1000 / 60 = 1.3333333333333333 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row '2,3-Pentanedione', TBER1 column group: Km = 385 uM; kcat = 29 min^-1; printed kcat/Km = 0.08 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row '2,3-Pentanedione', TBER1 column group


column_label: Table 1, Reduction section, substrate row '2,3-Pentanedione'; Rat 17HSD5 column 'Km (uM)' = 57; Rat 17HSD5 column 'kcat (min^-1)' = 8.8; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.15.

normalized_value_unit_evidence: Km: printed cell 57 uM; 57 / 1000 = 0.057 mM; the exported value is 0.057 mM. kcat: printed cell 8.8 min^-1; the printed turnover column is per MINUTE, so 8.8 / 60 = 0.14666666666666667 s^(-1); the exported value is 0.1466666667 s^(-1). kcat/Km: printed cell 0.15 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.15 x 1000 / 60 = 2.5 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row '2,3-Pentanedione', Rat 17HSD5 column group: Km = 57 uM; kcat = 8.8 min^-1; printed kcat/Km = 0.15 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row '2,3-Pentanedione', Rat 17HSD5 column group


smiles: CC(=O)C(=O)C

column_label: Table 1, Reduction section, substrate row '2,3-Butanedione'; TBER1 column 'Km (uM)' = 4800; TBER1 column 'kcat (min^-1)' = 100; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.02.

normalized_value_unit_evidence: Km: printed cell 4800 uM; 4800 / 1000 = 4.8 mM; the exported value is 4.8 mM. kcat: printed cell 100 min^-1; the printed turnover column is per MINUTE, so 100 / 60 = 1.6666666666666667 s^(-1); the exported value is 1.6666666667 s^(-1). kcat/Km: printed cell 0.02 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.02 x 1000 / 60 = 0.3333333333333333 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row '2,3-Butanedione', TBER1 column group: Km = 4800 uM; kcat = 100 min^-1; printed kcat/Km = 0.02 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row '2,3-Butanedione', TBER1 column group


column_label: Table 1, Reduction section, substrate row '2,3-Butanedione'; Rat 17HSD5 column 'Km (uM)' = 126; Rat 17HSD5 column 'kcat (min^-1)' = 16; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.13.

normalized_value_unit_evidence: Km: printed cell 126 uM; 126 / 1000 = 0.126 mM; the exported value is 0.126 mM. kcat: printed cell 16 min^-1; the printed turnover column is per MINUTE, so 16 / 60 = 0.26666666666666666 s^(-1); the exported value is 0.2666666667 s^(-1). kcat/Km: printed cell 0.13 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.13 x 1000 / 60 = 2.1666666666666665 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row '2,3-Butanedione', Rat 17HSD5 column group: Km = 126 uM; kcat = 16 min^-1; printed kcat/Km = 0.13 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row '2,3-Butanedione', Rat 17HSD5 column group


substrate_name: Acetylpyridine

column_label: Table 1, Reduction section, substrate row 'Acetylpyridine'; Rat 17HSD5 column 'Km (uM)' = 266; Rat 17HSD5 column 'kcat (min^-1)' = 4.5; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.02.

normalized_value_unit_evidence: Km: printed cell 266 uM; 266 / 1000 = 0.266 mM; the exported value is 0.266 mM. kcat: printed cell 4.5 min^-1; the printed turnover column is per MINUTE, so 4.5 / 60 = 0.075 s^(-1); the exported value is 0.075 s^(-1). kcat/Km: printed cell 0.02 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.02 x 1000 / 60 = 0.3333333333333333 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row 'Acetylpyridine', Rat 17HSD5 column group: Km = 266 uM; kcat = 4.5 min^-1; printed kcat/Km = 0.02 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row 'Acetylpyridine', Rat 17HSD5 column group

substrate: Acetylpyridine


column_label: Table 2, (A) section, substrate row '5b-Androstane-3a,17b-diol'; TBER1 column 'Km (uM)' = 3.5; TBER1 column 'kcat (min^-1)' = 28; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 8.1.

normalized_value_unit_evidence: Km: printed cell 3.5 uM; 3.5 / 1000 = 0.0035 mM; the exported value is 0.0035 mM. kcat: printed cell 28 min^-1; the printed turnover column is per MINUTE, so 28 / 60 = 0.4666666666666667 s^(-1); the exported value is 0.4666666667 s^(-1). kcat/Km: printed cell 8.1 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 8.1 x 1000 / 60 = 135.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row '5b-Androstane-3a,17b-diol', TBER1 column group: Km = 3.5 uM; kcat = 28 min^-1; printed kcat/Km = 8.1 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row '5b-Androstane-3a,17b-diol', TBER1 column group

table_label: Table 2: Substrate specificity for steroids in the oxidation and reduction reactions catalyzed by TBER1 and rat 17HSD5 (J. Biochem. 2006, 139(6):1053-1063, journal page 1058)


column_label: Table 2, (A) section, substrate row '5b-Androstane-3a,17b-diol'; Rat 17HSD5 column 'Km (uM)' = 10; Rat 17HSD5 column 'kcat (min^-1)' = 7.8; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.78.

normalized_value_unit_evidence: Km: printed cell 10 uM; 10 / 1000 = 0.01 mM; the exported value is 0.01 mM. kcat: printed cell 7.8 min^-1; the printed turnover column is per MINUTE, so 7.8 / 60 = 0.13 s^(-1); the exported value is 0.13 s^(-1). kcat/Km: printed cell 0.78 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.78 x 1000 / 60 = 13.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row '5b-Androstane-3a,17b-diol', Rat 17HSD5 column group: Km = 10 uM; kcat = 7.8 min^-1; printed kcat/Km = 0.78 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row '5b-Androstane-3a,17b-diol', Rat 17HSD5 column group


column_label: Table 2, (A) section, substrate row '5a-Dihydrotestosterone'; TBER1 column 'Km (uM)' = 9.1; TBER1 column 'kcat (min^-1)' = 12; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 1.30.

normalized_value_unit_evidence: Km: printed cell 9.1 uM; 9.1 / 1000 = 0.0091 mM; the exported value is 0.0091 mM. kcat: printed cell 12 min^-1; the printed turnover column is per MINUTE, so 12 / 60 = 0.2 s^(-1); the exported value is 0.2 s^(-1). kcat/Km: printed cell 1.30 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 1.30 x 1000 / 60 = 21.666666666666668 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row '5a-Dihydrotestosterone', TBER1 column group: Km = 9.1 uM; kcat = 12 min^-1; printed kcat/Km = 1.30 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row '5a-Dihydrotestosterone', TBER1 column group


substrate_name: Testosterone

column_label: Table 2, (A) section, substrate row 'Testosterone'; TBER1 column 'Km (uM)' = 25; TBER1 column 'kcat (min^-1)' = 16; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.62.

normalized_value_unit_evidence: Km: printed cell 25 uM; 25 / 1000 = 0.025 mM; the exported value is 0.025 mM. kcat: printed cell 16 min^-1; the printed turnover column is per MINUTE, so 16 / 60 = 0.26666666666666666 s^(-1); the exported value is 0.2666666667 s^(-1). kcat/Km: printed cell 0.62 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.62 x 1000 / 60 = 10.333333333333334 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row 'Testosterone', TBER1 column group: Km = 25 uM; kcat = 16 min^-1; printed kcat/Km = 0.62 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row 'Testosterone', TBER1 column group

substrate: Testosterone


column_label: Table 2, (A) section, substrate row 'Testosterone'; Rat 17HSD5 column 'Km (uM)' = 21; Rat 17HSD5 column 'kcat (min^-1)' = 21; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 1.0.

normalized_value_unit_evidence: Km: printed cell 21 uM; 21 / 1000 = 0.021 mM; the exported value is 0.021 mM. kcat: printed cell 21 min^-1; the printed turnover column is per MINUTE, so 21 / 60 = 0.35 s^(-1); the exported value is 0.35 s^(-1). kcat/Km: printed cell 1.0 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 1.0 x 1000 / 60 = 16.666666666666668 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row 'Testosterone', Rat 17HSD5 column group: Km = 21 uM; kcat = 21 min^-1; printed kcat/Km = 1.0 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row 'Testosterone', Rat 17HSD5 column group


column_label: Table 2, (A) section, substrate row '17b-Estradiol'; TBER1 column 'Km (uM)' = 33; TBER1 column 'kcat (min^-1)' = 9.0; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.27.

normalized_value_unit_evidence: Km: printed cell 33 uM; 33 / 1000 = 0.033 mM; the exported value is 0.033 mM. kcat: printed cell 9.0 min^-1; the printed turnover column is per MINUTE, so 9.0 / 60 = 0.15 s^(-1); the exported value is 0.15 s^(-1). kcat/Km: printed cell 0.27 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.27 x 1000 / 60 = 4.5 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row '17b-Estradiol', TBER1 column group: Km = 33 uM; kcat = 9.0 min^-1; printed kcat/Km = 0.27 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row '17b-Estradiol', TBER1 column group


column_label: Table 2, (A) section, substrate row '17b-Estradiol'; Rat 17HSD5 column 'Km (uM)' = 14; Rat 17HSD5 column 'kcat (min^-1)' = 25; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 1.8.

normalized_value_unit_evidence: Km: printed cell 14 uM; 14 / 1000 = 0.014 mM; the exported value is 0.014 mM. kcat: printed cell 25 min^-1; the printed turnover column is per MINUTE, so 25 / 60 = 0.4166666666666667 s^(-1); the exported value is 0.4166666667 s^(-1). kcat/Km: printed cell 1.8 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 1.8 x 1000 / 60 = 30.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row '17b-Estradiol', Rat 17HSD5 column group: Km = 14 uM; kcat = 25 min^-1; printed kcat/Km = 1.8 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row '17b-Estradiol', Rat 17HSD5 column group


column_label: Table 2, (A) section, substrate row '5a-Androstane-3a,17b-diol'; TBER1 column 'Km (uM)' = 17; TBER1 column 'kcat (min^-1)' = 4.2; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.25.

normalized_value_unit_evidence: Km: printed cell 17 uM; 17 / 1000 = 0.017 mM; the exported value is 0.017 mM. kcat: printed cell 4.2 min^-1; the printed turnover column is per MINUTE, so 4.2 / 60 = 0.07 s^(-1); the exported value is 0.07 s^(-1). kcat/Km: printed cell 0.25 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.25 x 1000 / 60 = 4.166666666666667 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row '5a-Androstane-3a,17b-diol', TBER1 column group: Km = 17 uM; kcat = 4.2 min^-1; printed kcat/Km = 0.25 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row '5a-Androstane-3a,17b-diol', TBER1 column group


column_label: Table 2, (B) section, substrate row '5a-Pregnan-20a-ol-3-one'; TBER1 column 'Km (uM)' = 5.1; TBER1 column 'kcat (min^-1)' = 2.4; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.47.

normalized_value_unit_evidence: Km: printed cell 5.1 uM; 5.1 / 1000 = 0.0050999999999999995 mM; the exported value is 0.0050999999999999995 mM. kcat: printed cell 2.4 min^-1; the printed turnover column is per MINUTE, so 2.4 / 60 = 0.04 s^(-1); the exported value is 0.04 s^(-1). kcat/Km: printed cell 0.47 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.47 x 1000 / 60 = 7.833333333333333 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (B) row '5a-Pregnan-20a-ol-3-one', TBER1 column group: Km = 5.1 uM; kcat = 2.4 min^-1; printed kcat/Km = 0.47 min^-1 uM^-1.

row_label: Table 2, (B) section, substrate row '5a-Pregnan-20a-ol-3-one', TBER1 column group


column_label: Table 2, (B) section, substrate row '5b-Pregnan-3a-ol-20-one'; TBER1 column 'Km (uM)' = 8.4; TBER1 column 'kcat (min^-1)' = 0.83; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.10.

normalized_value_unit_evidence: Km: printed cell 8.4 uM; 8.4 / 1000 = 0.008400000000000001 mM; the exported value is 0.008400000000000001 mM. kcat: printed cell 0.83 min^-1; the printed turnover column is per MINUTE, so 0.83 / 60 = 0.013833333333333333 s^(-1); the exported value is 0.0138333333 s^(-1). kcat/Km: printed cell 0.10 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.10 x 1000 / 60 = 1.6666666666666667 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (B) row '5b-Pregnan-3a-ol-20-one', TBER1 column group: Km = 8.4 uM; kcat = 0.83 min^-1; printed kcat/Km = 0.10 min^-1 uM^-1.

row_label: Table 2, (B) section, substrate row '5b-Pregnan-3a-ol-20-one', TBER1 column group


column_label: Table 2, (C) section, substrate row '5b-Androstan-3a-ol-17-one'; Rat 17HSD5 column 'Km (uM)' = 6.7; Rat 17HSD5 column 'kcat (min^-1)' = 1.3; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.20.

normalized_value_unit_evidence: Km: printed cell 6.7 uM; 6.7 / 1000 = 0.0067 mM; the exported value is 0.0067 mM. kcat: printed cell 1.3 min^-1; the printed turnover column is per MINUTE, so 1.3 / 60 = 0.021666666666666667 s^(-1); the exported value is 0.0216666667 s^(-1). kcat/Km: printed cell 0.20 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.20 x 1000 / 60 = 3.3333333333333335 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (C) row '5b-Androstan-3a-ol-17-one', Rat 17HSD5 column group (the second of the two section (C) rows carrying the substrate label '5b-Androstan-3a-ol-17-one'; it is the row that prints Rat 17HSD5 cells, the first printing NS in the Rat 17HSD5 group): Km = 6.7 uM; kcat = 1.3 min^-1; printed kcat/Km = 0.20 min^-1 uM^-1.

row_label: Table 2, (C) section, substrate row '5b-Androstan-3a-ol-17-one', Rat 17HSD5 column group


column_label: Table 2, (C) section, substrate row '5b-Androstane-3,17-dione'; TBER1 column 'Km (uM)' = 19; TBER1 column 'kcat (min^-1)' = 3.2; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.17.

normalized_value_unit_evidence: Km: printed cell 19 uM; 19 / 1000 = 0.019 mM; the exported value is 0.019 mM. kcat: printed cell 3.2 min^-1; the printed turnover column is per MINUTE, so 3.2 / 60 = 0.05333333333333334 s^(-1); the exported value is 0.0533333333 s^(-1). kcat/Km: printed cell 0.17 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.17 x 1000 / 60 = 2.8333333333333335 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (C) row '5b-Androstane-3,17-dione', TBER1 column group: Km = 19 uM; kcat = 3.2 min^-1; printed kcat/Km = 0.17 min^-1 uM^-1.

row_label: Table 2, (C) section, substrate row '5b-Androstane-3,17-dione', TBER1 column group


column_label: Table 2, (C) section, substrate row '5a-Androstane-3,17-dione'; TBER1 column 'Km (uM)' = 18; TBER1 column 'kcat (min^-1)' = 1.3; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.07.

normalized_value_unit_evidence: Km: printed cell 18 uM; 18 / 1000 = 0.018 mM; the exported value is 0.018 mM. kcat: printed cell 1.3 min^-1; the printed turnover column is per MINUTE, so 1.3 / 60 = 0.021666666666666667 s^(-1); the exported value is 0.0216666667 s^(-1). kcat/Km: printed cell 0.07 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.07 x 1000 / 60 = 1.1666666666666667 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (C) row '5a-Androstane-3,17-dione', TBER1 column group: Km = 18 uM; kcat = 1.3 min^-1; printed kcat/Km = 0.07 min^-1 uM^-1.

row_label: Table 2, (C) section, substrate row '5a-Androstane-3,17-dione', TBER1 column group


column_label: Table 2, (C) section, substrate row '4-Androstene-3,17-dione'; TBER1 column 'Km (uM)' = 17; TBER1 column 'kcat (min^-1)' = 1.0; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.06.

normalized_value_unit_evidence: Km: printed cell 17 uM; 17 / 1000 = 0.017 mM; the exported value is 0.017 mM. kcat: printed cell 1.0 min^-1; the printed turnover column is per MINUTE, so 1.0 / 60 = 0.016666666666666666 s^(-1); the exported value is 0.0166666667 s^(-1). kcat/Km: printed cell 0.06 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.06 x 1000 / 60 = 1.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (C) row '4-Androstene-3,17-dione', TBER1 column group: Km = 17 uM; kcat = 1.0 min^-1; printed kcat/Km = 0.06 min^-1 uM^-1.

row_label: Table 2, (C) section, substrate row '4-Androstene-3,17-dione', TBER1 column group


substrate_name: Dehydroepiandrosterone

column_label: Table 2, (C) section, substrate row 'Dehydroepiandrosterone'; Rat 17HSD5 column 'Km (uM)' = 11; Rat 17HSD5 column 'kcat (min^-1)' = 5.0; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.45.

normalized_value_unit_evidence: Km: printed cell 11 uM; 11 / 1000 = 0.011 mM; the exported value is 0.011 mM. kcat: printed cell 5.0 min^-1; the printed turnover column is per MINUTE, so 5.0 / 60 = 0.08333333333333333 s^(-1); the exported value is 0.0833333333 s^(-1). kcat/Km: printed cell 0.45 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.45 x 1000 / 60 = 7.5 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (C) row 'Dehydroepiandrosterone', Rat 17HSD5 column group: Km = 11 uM; kcat = 5.0 min^-1; printed kcat/Km = 0.45 min^-1 uM^-1.

row_label: Table 2, (C) section, substrate row 'Dehydroepiandrosterone', Rat 17HSD5 column group

substrate: Dehydroepiandrosterone


column_label: Table 2, (D) section, substrate row '17a-Hydroxyprogesterone'; Rat 17HSD5 column 'Km (uM)' = 50; Rat 17HSD5 column 'kcat (min^-1)' = 6.2; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.12.

normalized_value_unit_evidence: Km: printed cell 50 uM; 50 / 1000 = 0.05 mM; the exported value is 0.05 mM. kcat: printed cell 6.2 min^-1; the printed turnover column is per MINUTE, so 6.2 / 60 = 0.10333333333333333 s^(-1); the exported value is 0.1033333333 s^(-1). kcat/Km: printed cell 0.12 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.12 x 1000 / 60 = 2.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (D) row '17a-Hydroxyprogesterone', Rat 17HSD5 column group: Km = 50 uM; kcat = 6.2 min^-1; printed kcat/Km = 0.12 min^-1 uM^-1.

row_label: Table 2, (D) section, substrate row '17a-Hydroxyprogesterone', Rat 17HSD5 column group


column_label: Table 2, (E) section, substrate row '16-Ketoestrone'; TBER1 column 'Km (uM)' = 0.4; TBER1 column 'kcat (min^-1)' = 113; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 283.

normalized_value_unit_evidence: Km: printed cell 0.4 uM; 0.4 / 1000 = 0.0004 mM; the exported value is 0.0004 mM. kcat: printed cell 113 min^-1; the printed turnover column is per MINUTE, so 113 / 60 = 1.8833333333333333 s^(-1); the exported value is 1.8833333333 s^(-1). kcat/Km: printed cell 283 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 283 x 1000 / 60 = 4716.666666666667 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (E) row '16-Ketoestrone', TBER1 column group: Km = 0.4 uM; kcat = 113 min^-1; printed kcat/Km = 283 min^-1 uM^-1.

row_label: Table 2, (E) section, substrate row '16-Ketoestrone', TBER1 column group


column_label: Table 2, (E) section, substrate row '16-Ketoestrone'; Rat 17HSD5 column 'Km (uM)' = 0.7; Rat 17HSD5 column 'kcat (min^-1)' = 5.2; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 7.5.

normalized_value_unit_evidence: Km: printed cell 0.7 uM; 0.7 / 1000 = 0.0007 mM; the exported value is 0.0007 mM. kcat: printed cell 5.2 min^-1; the printed turnover column is per MINUTE, so 5.2 / 60 = 0.08666666666666667 s^(-1); the exported value is 0.0866666667 s^(-1). kcat/Km: printed cell 7.5 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 7.5 x 1000 / 60 = 125.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (E) row '16-Ketoestrone', Rat 17HSD5 column group: Km = 0.7 uM; kcat = 5.2 min^-1; printed kcat/Km = 7.5 min^-1 uM^-1.

row_label: Table 2, (E) section, substrate row '16-Ketoestrone', Rat 17HSD5 column group


column_label: Table 2, (E) section, substrate row '21-Dehydrocortisol'; Rat 17HSD5 column 'Km (uM)' = 2.8; Rat 17HSD5 column 'kcat (min^-1)' = 7.2; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 2.6.

normalized_value_unit_evidence: Km: printed cell 2.8 uM; 2.8 / 1000 = 0.0028 mM; the exported value is 0.0028 mM. kcat: printed cell 7.2 min^-1; the printed turnover column is per MINUTE, so 7.2 / 60 = 0.12000000000000001 s^(-1); the exported value is 0.12 s^(-1). kcat/Km: printed cell 2.6 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 2.6 x 1000 / 60 = 43.333333333333336 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (E) row '21-Dehydrocortisol', Rat 17HSD5 column group: Km = 2.8 uM; kcat = 7.2 min^-1; printed kcat/Km = 2.6 min^-1 uM^-1.

row_label: Table 2, (E) section, substrate row '21-Dehydrocortisol', Rat 17HSD5 column group


assay_conditions_summary: pH 7.4; 298.15 K; cofactor NADPH

column_label: Journal page 1059, the printed Km and kcat for the NADPH-linked reduction of TBE by the recombinant rat 3a-HSD: Km = 23 uM; kcat = 24 min^-1. The paper prints no kcat/Km value for this measurement.

normalized_value_unit_evidence: Km: printed cell 23 uM; 23 / 1000 = 0.023 mM; the exported value is 0.023 mM. kcat: printed cell 24 min^-1; the printed turnover column is per MINUTE, so 24 / 60 = 0.4 s^(-1); the exported value is 0.4 s^(-1). kcat/Km: the paper prints no catalytic-efficiency value for this measurement and this catalog row carries none either, so the ratio is typed as the same-measurement quotient of the two printed cells, 0.4 / 0.023 = 17.39130434782609 s^(-1)*mM^(-1), and no ratio is shipped.

raw_value_unit_evidence: journal page 1059, the printed Km and kcat for the NADPH-linked reduction of TBE by the recombinant rat 3a-HSD: Km = 23 uM; kcat = 24 min^-1; the paper prints no kcat/Km value for this measurement.

table_label: Identity of TBER2 with 3a-HSD: the printed kinetic constants of the recombinant rat 3a-HSD (J. Biochem. 2006, 139(6):1053-1063, journal page 1059)


substrate_name: trans-benzene dihydrodiol

column_label: Journal page 1059, the printed Km and kcat for the oxidation of trans-benzene dihydrodiol by the recombinant rat 3a-HSD: Km = 0.37 mM; kcat = 3.6 min^-1. The paper prints no kcat/Km value for this measurement.

normalized_value_unit_evidence: Km: printed cell 0.37 mM; 0.37 mM needs no conversion; the exported value is 0.37 mM. kcat: printed cell 3.6 min^-1; the printed turnover column is per MINUTE, so 3.6 / 60 = 0.060000000000000005 s^(-1); the exported value is 0.06 s^(-1). kcat/Km: the paper prints no catalytic-efficiency value for this measurement and this catalog row carries none either, so the ratio is typed as the same-measurement quotient of the two printed cells, 0.060000000000000005 / 0.37 = 0.16216216216216217 s^(-1)*mM^(-1), and no ratio is shipped.

raw_value_unit_evidence: journal page 1059, the printed Km and kcat for the oxidation of trans-benzene dihydrodiol by the recombinant rat 3a-HSD: Km = 0.37 mM; kcat = 3.6 min^-1; the paper prints no kcat/Km value for this measurement.

substrate: trans-benzene dihydrodiol


enzyme_display_name: 4-hydroxyproline epimerase

organism: Paracoccus denitrificans

verification_status: unverified

enzyme: 4-hydroxyproline epimerase

status: unverified


organism: Shewanella loihica


organism: Xanthomonas campestris


organism: Ochrobactrum anthropi


organism: Agrobacterium tumefaciens


organism: Burkholderia ambifaria


organism: Burkholderia multivorans


organism: Bacillus cereus


organism: Burkholderia thailandensis


enzyme_display_name: alcohol dehydrogenase (NADP+)

organism: Homo sapiens

enzyme: alcohol dehydrogenase (NADP+)


enzyme_display_name: beta-lactamase

organism: Stenotrophomonas maltophilia

substrate_name: Carbenicillin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Carbenicillin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 194 +/- 15; column 'kcat (s-1)' = 277; column 'kcat/Km (M-1 s-1)' = 1.40 x 10^6.

normalized_value_unit_evidence: Km: printed Table 3 cell 194 uM; 194 / 1000 = 0.194 mM; the exported value is 0.194 mM. kcat: printed Table 3 cell 277 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 277 s^(-1). kcat/Km: printed Table 3 cell 1.40 x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1400000 / 1000 = 1400 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Carbenicillin': Km = 194 +/- 15 uM; kcat = 277 s^-1; printed kcat/Km = 1.40 x 10^6 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Carbenicillin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

table_label: Table 3: Kinetic parameters determined for class B beta-lactamases (Biochemical Journal 1993, 291:151-155)

enzyme: beta-lactamase

substrate: Carbenicillin


substrate_name: Penicillin G

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Penicillin G' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 50 +/- 6; column 'kcat (s-1)' = 1100; column 'kcat/Km (M-1 s-1)' = 2.20 x 10^7.

normalized_value_unit_evidence: Km: printed Table 3 cell 50 uM; 50 / 1000 = 0.05 mM; the exported value is 0.05 mM. kcat: printed Table 3 cell 1100 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 1100 s^(-1). kcat/Km: printed Table 3 cell 2.20 x 10^7 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 22000000 / 1000 = 22000 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Penicillin G': Km = 50 +/- 6 uM; kcat = 1100 s^-1; printed kcat/Km = 2.20 x 10^7 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Penicillin G' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Penicillin G


substrate_name: Oxacillin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Oxacillin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 27 +/- 3; column 'kcat (s-1)' = 285; column 'kcat/Km (M-1 s-1)' = 1.10 x 10^7.

normalized_value_unit_evidence: Km: printed Table 3 cell 27 uM; 27 / 1000 = 0.027 mM; the exported value is 0.027 mM. kcat: printed Table 3 cell 285 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 285 s^(-1). kcat/Km: printed Table 3 cell 1.10 x 10^7 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 11000000 / 1000 = 11000 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Oxacillin': Km = 27 +/- 3 uM; kcat = 285 s^-1; printed kcat/Km = 1.10 x 10^7 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Oxacillin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Oxacillin


substrate_name: Cephaloridin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cephaloridin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 300 +/- 50; column 'kcat (s-1)' = 28; column 'kcat/Km (M-1 s-1)' = 9.30 x 10^4.

normalized_value_unit_evidence: Km: printed Table 3 cell 300 uM; 300 / 1000 = 0.3 mM; the exported value is 0.3 mM. kcat: printed Table 3 cell 28 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 28 s^(-1). kcat/Km: printed Table 3 cell 9.30 x 10^4 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 93000 / 1000 = 93 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Cephaloridin': Km = 300 +/- 50 uM; kcat = 28 s^-1; printed kcat/Km = 9.30 x 10^4 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cephaloridin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Cephaloridin


substrate_name: Cephalexin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cephalexin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 100 +/- 8; column 'kcat (s-1)' = 37; column 'kcat/Km (M-1 s-1)' = 3.70 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 100 uM; 100 / 1000 = 0.1 mM; the exported value is 0.1 mM. kcat: printed Table 3 cell 37 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 37 s^(-1). kcat/Km: printed Table 3 cell 3.70 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 370000 / 1000 = 370 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Cephalexin': Km = 100 +/- 8 uM; kcat = 37 s^-1; printed kcat/Km = 3.70 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cephalexin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Cephalexin


substrate_name: Cephaloglycin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cephaloglycin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 36 +/- 2; column 'kcat (s-1)' = 19; column 'kcat/Km (M-1 s-1)' = 5.30 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 36 uM; 36 / 1000 = 0.036 mM; the exported value is 0.036 mM. kcat: printed Table 3 cell 19 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 19 s^(-1). kcat/Km: printed Table 3 cell 5.30 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 530000 / 1000 = 530 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Cephaloglycin': Km = 36 +/- 2 uM; kcat = 19 s^-1; printed kcat/Km = 5.30 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cephaloglycin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Cephaloglycin


substrate_name: Nitrocefin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Nitrocefin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 7 +/- 1; column 'kcat (s-1)' = 20; column 'kcat/Km (M-1 s-1)' = 2.90 x 10^6.

normalized_value_unit_evidence: Km: printed Table 3 cell 7 uM; 7 / 1000 = 0.007 mM; the exported value is 0.007 mM. kcat: printed Table 3 cell 20 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 20 s^(-1). kcat/Km: printed Table 3 cell 2.90 x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 2900000 / 1000 = 2900 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Nitrocefin': Km = 7 +/- 1 uM; kcat = 20 s^-1; printed kcat/Km = 2.90 x 10^6 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Nitrocefin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Nitrocefin


substrate_name: Cefuroxime

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cefuroxime' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 30 +/- 3; column 'kcat (s-1)' = 80; column 'kcat/Km (M-1 s-1)' = 2.70 x 10^6.

normalized_value_unit_evidence: Km: printed Table 3 cell 30 uM; 30 / 1000 = 0.03 mM; the exported value is 0.03 mM. kcat: printed Table 3 cell 80 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 80 s^(-1). kcat/Km: printed Table 3 cell 2.70 x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 2700000 / 1000 = 2700 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Cefuroxime': Km = 30 +/- 3 uM; kcat = 80 s^-1; printed kcat/Km = 2.70 x 10^6 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cefuroxime' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Cefuroxime


substrate_name: Cefotaxime

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cefotaxime' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 26 +/- 3; column 'kcat (s-1)' = 66; column 'kcat/Km (M-1 s-1)' = 2.60 x 10^6.

normalized_value_unit_evidence: Km: printed Table 3 cell 26 uM; 26 / 1000 = 0.026 mM; the exported value is 0.026 mM. kcat: printed Table 3 cell 66 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 66 s^(-1). kcat/Km: printed Table 3 cell 2.60 x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 2600000 / 1000 = 2600 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Cefotaxime': Km = 26 +/- 3 uM; kcat = 66 s^-1; printed kcat/Km = 2.60 x 10^6 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cefotaxime' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Cefotaxime


substrate_name: Imipenem

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Imipenem' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 90 +/- 8; column 'kcat (s-1)' = 65; column 'kcat/Km (M-1 s-1)' = 7.30 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 90 uM; 90 / 1000 = 0.09 mM; the exported value is 0.09 mM. kcat: printed Table 3 cell 65 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 65 s^(-1). kcat/Km: printed Table 3 cell 7.30 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 730000 / 1000 = 730 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Imipenem': Km = 90 +/- 8 uM; kcat = 65 s^-1; printed kcat/Km = 7.30 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Imipenem' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Imipenem


substrate_name: Sulbactam

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Sulbactam' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 76 +/- 4; column 'kcat (s-1)' = 210; column 'kcat/Km (M-1 s-1)' = 2.80 x 10^6.

normalized_value_unit_evidence: Km: printed Table 3 cell 76 uM; 76 / 1000 = 0.076 mM; the exported value is 0.076 mM. kcat: printed Table 3 cell 210 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 210 s^(-1). kcat/Km: printed Table 3 cell 2.80 x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 2800000 / 1000 = 2800 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Sulbactam': Km = 76 +/- 4 uM; kcat = 210 s^-1; printed kcat/Km = 2.80 x 10^6 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Sulbactam' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Sulbactam


substrate_name: Cefoxitin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cefoxitin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 2 +/- 0.05; column 'kcat (s-1)' = 1.10; column 'kcat/Km (M-1 s-1)' = 5.50 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 2 uM; 2 / 1000 = 0.002 mM; the exported value is 0.002 mM. kcat: printed Table 3 cell 1.10 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 1.1 s^(-1). kcat/Km: printed Table 3 cell 5.50 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 550000 / 1000 = 550 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Cefoxitin': Km = 2 +/- 0.05 uM; kcat = 1.10 s^-1; printed kcat/Km = 5.50 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cefoxitin' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Cefoxitin


substrate_name: Moxalactam

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Moxalactam' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 1 +/- 0.05; column 'kcat (s-1)' = 0.29; column 'kcat/Km (M-1 s-1)' = 2.90 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 1 uM; 1 / 1000 = 0.001 mM; the exported value is 0.001 mM. kcat: printed Table 3 cell 0.29 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.29 s^(-1). kcat/Km: printed Table 3 cell 2.90 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 290000 / 1000 = 290 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row 'Moxalactam': Km = 1 +/- 0.05 uM; kcat = 0.29 s^-1; printed kcat/Km = 2.90 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Moxalactam' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group

substrate: Moxalactam


column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Carbenicillin' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 4200 +/- 300; column 'kcat (s-1)' = 755; column 'kcat/Km (M-1 s-1)' = 1.80 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 4200 uM; 4200 / 1000 = 4.2 mM; the exported value is 4.2 mM. kcat: printed Table 3 cell 755 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 755 s^(-1). kcat/Km: printed Table 3 cell 1.80 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 180000 / 1000 = 180 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Carbenicillin': Km = 4200 +/- 300 uM; kcat = 755 s^-1; printed kcat/Km = 1.80 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Carbenicillin' within the 'Bacillus cereus beta-lactamase II' column group


column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Oxacillin' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 1750 +/- 20; column 'kcat (s-1)' = 325; column 'kcat/Km (M-1 s-1)' = 1.90 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 1750 uM; 1750 / 1000 = 1.75 mM; the exported value is 1.75 mM. kcat: printed Table 3 cell 325 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 325 s^(-1). kcat/Km: printed Table 3 cell 1.90 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 190000 / 1000 = 190 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Oxacillin': Km = 1750 +/- 20 uM; kcat = 325 s^-1; printed kcat/Km = 1.90 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Oxacillin' within the 'Bacillus cereus beta-lactamase II' column group


column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cephalexin' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 300 +/- 26; column 'kcat (s-1)' = 1.7; column 'kcat/Km (M-1 s-1)' = 5.70 x 10^3.

normalized_value_unit_evidence: Km: printed Table 3 cell 300 uM; 300 / 1000 = 0.3 mM; the exported value is 0.3 mM. kcat: printed Table 3 cell 1.7 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 1.7 s^(-1). kcat/Km: printed Table 3 cell 5.70 x 10^3 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 5700 / 1000 = 5.7 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Cephalexin': Km = 300 +/- 26 uM; kcat = 1.7 s^-1; printed kcat/Km = 5.70 x 10^3 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cephalexin' within the 'Bacillus cereus beta-lactamase II' column group


column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cephaloglycin' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 190 +/- 5; column 'kcat (s-1)' = 61; column 'kcat/Km (M-1 s-1)' = 3.20 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 190 uM; 190 / 1000 = 0.19 mM; the exported value is 0.19 mM. kcat: printed Table 3 cell 61 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 61 s^(-1). kcat/Km: printed Table 3 cell 3.20 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 320000 / 1000 = 320 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Cephaloglycin': Km = 190 +/- 5 uM; kcat = 61 s^-1; printed kcat/Km = 3.20 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cephaloglycin' within the 'Bacillus cereus beta-lactamase II' column group


column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Nitrocefin' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 70 +/- 5; column 'kcat (s-1)' = 45; column 'kcat/Km (M-1 s-1)' = 6.40 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 70 uM; 70 / 1000 = 0.07 mM; the exported value is 0.07 mM. kcat: printed Table 3 cell 45 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 45 s^(-1). kcat/Km: printed Table 3 cell 6.40 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 640000 / 1000 = 640 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Nitrocefin': Km = 70 +/- 5 uM; kcat = 45 s^-1; printed kcat/Km = 6.40 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Nitrocefin' within the 'Bacillus cereus beta-lactamase II' column group


column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cefuroxime' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 45 +/- 5; column 'kcat (s-1)' = 35; column 'kcat/Km (M-1 s-1)' = 7.80 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 45 uM; 45 / 1000 = 0.045 mM; the exported value is 0.045 mM. kcat: printed Table 3 cell 35 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 35 s^(-1). kcat/Km: printed Table 3 cell 7.80 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 780000 / 1000 = 780 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Cefuroxime': Km = 45 +/- 5 uM; kcat = 35 s^-1; printed kcat/Km = 7.80 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cefuroxime' within the 'Bacillus cereus beta-lactamase II' column group


column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cefotaxime' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 90 +/- 10; column 'kcat (s-1)' = 60; column 'kcat/Km (M-1 s-1)' = 6.70 x 10^5.

normalized_value_unit_evidence: Km: printed Table 3 cell 90 uM; 90 / 1000 = 0.09 mM; the exported value is 0.09 mM. kcat: printed Table 3 cell 60 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 60 s^(-1). kcat/Km: printed Table 3 cell 6.70 x 10^5 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 670000 / 1000 = 670 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Cefotaxime': Km = 90 +/- 10 uM; kcat = 60 s^-1; printed kcat/Km = 6.70 x 10^5 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cefotaxime' within the 'Bacillus cereus beta-lactamase II' column group


column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Sulbactam' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 5200 +/- 325; column 'kcat (s-1)' = 10; column 'kcat/Km (M-1 s-1)' = 1.90 x 10^3.

normalized_value_unit_evidence: Km: printed Table 3 cell 5200 uM; 5200 / 1000 = 5.2 mM; the exported value is 5.2 mM. kcat: printed Table 3 cell 10 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 10 s^(-1). kcat/Km: printed Table 3 cell 1.90 x 10^3 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1900 / 1000 = 1.9 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Sulbactam': Km = 5200 +/- 325 uM; kcat = 10 s^-1; printed kcat/Km = 1.90 x 10^3 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Sulbactam' within the 'Bacillus cereus beta-lactamase II' column group


column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cefoxitin' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 2100 +/- 465; column 'kcat (s-1)' = 0.2; column 'kcat/Km (M-1 s-1)' = 9.50 x 10^1.

normalized_value_unit_evidence: Km: printed Table 3 cell 2100 uM; 2100 / 1000 = 2.1 mM; the exported value is 2.1 mM. kcat: printed Table 3 cell 0.2 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.2 s^(-1). kcat/Km: printed Table 3 cell 9.50 x 10^1 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 95 / 1000 = 0.095 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Cefoxitin': Km = 2100 +/- 465 uM; kcat = 0.2 s^-1; printed kcat/Km = 9.50 x 10^1 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cefoxitin' within the 'Bacillus cereus beta-lactamase II' column group


column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Moxalactam' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 644 +/- 90; column 'kcat (s-1)' = 1.2; column 'kcat/Km (M-1 s-1)' = 1.90 x 10^3.

normalized_value_unit_evidence: Km: printed Table 3 cell 644 uM; 644 / 1000 = 0.644 mM; the exported value is 0.644 mM. kcat: printed Table 3 cell 1.2 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 1.2 s^(-1). kcat/Km: printed Table 3 cell 1.90 x 10^3 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1900 / 1000 = 1.9 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row 'Moxalactam': Km = 644 +/- 90 uM; kcat = 1.2 s^-1; printed kcat/Km = 1.90 x 10^3 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Moxalactam' within the 'Bacillus cereus beta-lactamase II' column group


organism: Mycobacterium tuberculosis

substrate_name: Cephaloridine

assay_conditions_summary: pH 6; 310.15 K; buffer 0.1 M sodium phosphate

column_label: Table 3; measurement recombinant Y49 b-lactamase cephaloridine row; construct recombinant Y49 b-lactamase; substrate cephaloridine; assayed in 0.1 M sodium phosphate buffer pH 6.0 at 37 C. kcat 8 s^-1. Km 798 uM. Relative Vmax 39. REH 2. No kcat/Km cell is printed in Table 3.

normalized_value_unit_evidence: Km: printed Table 3 cell 798 uM; 798 / 1000 = 0.798 mM; the exported value is 0.798 mM. kcat: printed Table 3 cell 8 s^-1; the printed unit is already s^-1, so no unit conversion applies; the exported value is 8.0 s^(-1).

raw_value_unit_evidence: Table 3, recombinant Y-49 b-lactamase block, cephaloridine row: Km = 798 uM; kcat = 8 s^-1; Relative Vmax = 39; REH = 2. Table 3 prints no catalytic-efficiency column and the Ki column is blank on this line.

table_label: Table 3: Kinetic characteristics of recombinant Y49 b-lactamase from E. coli and b-lactamase obtained directly from M. tuberculosis (Antimicrob. Agents Chemother. 1998, 42(6):1375-1381)

substrate: Cephaloridine


substrate_name: Cefazolin

column_label: Table 3; measurement recombinant Y49 b-lactamase cefazolin row; construct recombinant Y49 b-lactamase; substrate cefazolin; assayed in 0.1 M sodium phosphate buffer pH 6.0 at 37 C. kcat 8 s^-1. Km 490 uM. Relative Vmax 36. REH 4. No kcat/Km cell is printed in Table 3.

normalized_value_unit_evidence: Km: printed Table 3 cell 490 uM; 490 / 1000 = 0.49 mM; the exported value is 0.49 mM. kcat: printed Table 3 cell 8 s^-1; the printed unit is already s^-1, so no unit conversion applies; the exported value is 8.0 s^(-1).

raw_value_unit_evidence: Table 3, recombinant Y-49 b-lactamase block, cefazolin row: Km = 490 uM; kcat = 8 s^-1; Relative Vmax = 36; REH = 4. Table 3 prints no catalytic-efficiency column and the Ki column is blank on this line.

substrate: Cefazolin


substrate_name: Cephapirin

column_label: Table 3; measurement recombinant Y49 b-lactamase cephapirin row; construct recombinant Y49 b-lactamase; substrate cephapirin; assayed in 0.1 M sodium phosphate buffer pH 6.0 at 37 C. kcat 3 s^-1. Km 680 uM. Relative Vmax 15. REH 1. No kcat/Km cell is printed in Table 3.

normalized_value_unit_evidence: Km: printed Table 3 cell 680 uM; 680 / 1000 = 0.68 mM; the exported value is 0.68 mM. kcat: printed Table 3 cell 3 s^-1; the printed unit is already s^-1, so no unit conversion applies; the exported value is 3.0 s^(-1).

raw_value_unit_evidence: Table 3, recombinant Y-49 b-lactamase block, cephapirin row: Km = 680 uM; kcat = 3 s^-1; Relative Vmax = 15; REH = 1. Table 3 prints no catalytic-efficiency column and the Ki column is blank on this line.

substrate: Cephapirin


substrate_name: Cefamandole

column_label: Table 3; measurement recombinant Y49 b-lactamase cefamandole row; construct recombinant Y49 b-lactamase; substrate cefamandole; assayed in 0.1 M sodium phosphate buffer pH 6.0 at 37 C. kcat 22 s^-1. Km 645 uM. Relative Vmax 104. REH 8. No kcat/Km cell is printed in Table 3.

normalized_value_unit_evidence: Km: printed Table 3 cell 645 uM; 645 / 1000 = 0.645 mM; the exported value is 0.645 mM. kcat: printed Table 3 cell 22 s^-1; the printed unit is already s^-1, so no unit conversion applies; the exported value is 22.0 s^(-1).

raw_value_unit_evidence: Table 3, recombinant Y-49 b-lactamase block, cefamandole row: Km = 645 uM; kcat = 22 s^-1; Relative Vmax = 104; REH = 8. Table 3 prints no catalytic-efficiency column and the Ki column is blank on this line.

substrate: Cefamandole


organism: Citrobacter diversus

column_label: Table 4 (Substrate profile of C. diversus beta-lactamases), 'Form I' group, substrate row 'Cephaloridine'; column 'Km (uM)' = 740 +/- 70; column 'kcat (s-1)' = 930 +/- 100; column 'kcat/Km (M-1 s-1)' = (1.25 +/- 0.25) x 10^6.

normalized_value_unit_evidence: Km: printed Table 4 cell 740 +/- 70 uM; 740 / 1000 = 0.74 mM; the exported value is 0.74 mM. kcat: printed Table 4 cell 930 +/- 100 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 930 s^(-1). kcat/Km: printed Table 4 cell (1.25 +/- 0.25) x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1250000 / 1000 = 1250 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 4, 'Form I' group, substrate row 'Cephaloridine': Km = 740 +/- 70 uM; kcat = 930 +/- 100 s^-1; printed kcat/Km = (1.25 +/- 0.25) x 10^6 M^-1 s^-1.

table_label: Table 4: Substrate profile of C. diversus beta-lactamases (Biochemical Journal 1988, 254:885-890)


substrate_name: Cephalothin

column_label: Table 4 (Substrate profile of C. diversus beta-lactamases), 'Form I' group, substrate row 'Cephalothin'; column 'Km (uM)' = 48 +/- 3; column 'kcat (s-1)' = 63 +/- 3; column 'kcat/Km (M-1 s-1)' = (1.3 +/- 0.15) x 10^6.

normalized_value_unit_evidence: Km: printed Table 4 cell 48 +/- 3 uM; 48 / 1000 = 0.048 mM; the exported value is 0.048 mM. kcat: printed Table 4 cell 63 +/- 3 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 63 s^(-1). kcat/Km: printed Table 4 cell (1.3 +/- 0.15) x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1300000 / 1000 = 1300 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 4, 'Form I' group, substrate row 'Cephalothin': Km = 48 +/- 3 uM; kcat = 63 +/- 3 s^-1; printed kcat/Km = (1.3 +/- 0.15) x 10^6 M^-1 s^-1.

substrate: Cephalothin


substrate_name: Cephalosporin C

column_label: Table 4 (Substrate profile of C. diversus beta-lactamases), 'Form I' group, substrate row 'Cephalosporin C'; column 'Km (uM)' = 126 +/- 20; column 'kcat (s-1)' = 41 +/- 5; column 'kcat/Km (M-1 s-1)' = (0.3 +/- 0.06) x 10^6.

normalized_value_unit_evidence: Km: printed Table 4 cell 126 +/- 20 uM; 126 / 1000 = 0.126 mM; the exported value is 0.126 mM. kcat: printed Table 4 cell 41 +/- 5 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 41 s^(-1). kcat/Km: printed Table 4 cell (0.3 +/- 0.06) x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 300000 / 1000 = 300 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 4, 'Form I' group, substrate row 'Cephalosporin C': Km = 126 +/- 20 uM; kcat = 41 +/- 5 s^-1; printed kcat/Km = (0.3 +/- 0.06) x 10^6 M^-1 s^-1.

substrate: Cephalosporin C


enzyme_display_name: L-iditol 2-dehydrogenase

organism: Bos taurus

substrate_name: D-Fructose

enzyme: L-iditol 2-dehydrogenase

substrate: D-Fructose


substrate_name: NADH

substrate: NADH


enzyme_display_name: aldehyde dehydrogenase (NAD(+))

substrate_name: formaldehyde

smiles: C=O

enzyme: aldehyde dehydrogenase (NAD(+))

substrate: formaldehyde


substrate_name: acetaldehyde

smiles: CC=O

substrate: acetaldehyde


substrate_name: propanal

smiles: CCC=O

substrate: propanal


substrate_name: pentanal

smiles: CCCCC=O

substrate: pentanal


substrate_name: hexanal

smiles: CCCCCC=O

substrate: hexanal


substrate_name: heptanal

smiles: CCCCCCC=O

substrate: heptanal


substrate_name: decanal

smiles: CCCCCCCCCC=O

substrate: decanal


substrate_name: p-nitrocinnamaldehyde

substrate: p-nitrocinnamaldehyde


substrate_name: p-(dimethylamino)cinnamaldehyde

substrate: p-(dimethylamino)cinnamaldehyde


substrate_name: trans-cinnamaldehyde

substrate: trans-cinnamaldehyde


substrate_name: hydrocinnamaldehyde

substrate: hydrocinnamaldehyde


substrate_name: alpha-phenylpropionaldehyde

substrate: alpha-phenylpropionaldehyde


substrate_name: phenylacetaldehyde

substrate: phenylacetaldehyde


substrate_name: benzaldehyde

substrate: benzaldehyde


enzyme_display_name: 4-hydroxybenzoyl-CoA thioesterase

organism: Pseudomonas sp.

enzyme: 4-hydroxybenzoyl-CoA thioesterase


substrate_name: benzylpenicillin

assay_conditions_summary: pH 7; 293.15 K; buffer containing 0

column_label: Table 1: Rate constants for the hydrolysis of benzylpenicillin (Biochem. J. 1990, 272:613-619), wild-type column, substrate row 'benzylpenicillin'; column 'Km(UM), decoded as Km in uM' = 65; column 'kcat. (s-1)' = 2200; column 'kcat./Km (j#m1 s-.), decoded as kcat/Km in uM^-1 s^-1' = 34.

normalized_value_unit_evidence: Km: printed cell 65 uM; 65 / 1000 = 0.065 mM. kcat: printed cell 2200 s^-1; already in s^-1, no unit conversion applies, so the cell is 2200.0 s^(-1). kcat/Km: printed cell 34 uM^-1 s^-1; 1 uM^-1 s^-1 is 1000 s^-1 mM^-1, so 34 x 1000 = 34000.0 s^-1 mM^-1.

raw_value_unit_evidence: Table 1. Rate constants for the hydrolysis of benzylpenicillin, wild-type column, substrate row 'benzylpenicillin': Km = 65 uM; kcat = 2200 s^-1; printed kcat/Km = 34 uM^-1 s^-1.

row_label: Table 1, wild-type column, hydrolysis of benzylpenicillin

substrate: benzylpenicillin


organism: Ovis aries

substrate_name: Glycol

smiles: OCCO

substrate: Glycol


substrate_name: Glycerol

smiles: C(C(CO)O)O

substrate: Glycerol


substrate_name: L-threitol

smiles: C(C(C(CO)O)O)O

substrate: L-threitol


substrate_name: D-threitol

substrate: D-threitol


substrate_name: L-arabitol

substrate: L-arabitol


substrate_name: D-arabitol

substrate: D-arabitol


substrate_name: meso-galactitol

smiles: C(C(C(C(C(CO)O)O)O)O)O

substrate: meso-galactitol


substrate_name: L-glucitol

substrate: L-glucitol


substrate_name: L-mannitol

substrate: L-mannitol


substrate_name: D-mannitol

substrate: D-mannitol


substrate_name: L-altritol

smiles: OCC(O)C(O)C(O)C(O)CO

substrate: L-altritol


substrate_name: D-altritol

substrate: D-altritol


substrate_name: L-iditol

substrate: L-iditol


substrate_name: D-iditol

substrate: D-iditol


substrate_name: D-glycero-L-allo-heptitol

substrate: D-glycero-L-allo-heptitol


substrate_name: D-glycero-L-gulo-heptitol

smiles: C(C(C(C(C(C(CO)O)O)O)O)O)O

substrate: D-glycero-L-gulo-heptitol


smiles: CC(CO)O


smiles: C=CC(O)CO


organism: Rattus norvegicus

column_label: Table 1, Oxidation section, substrate row 'S-Indan-1-ol'; TBER1 column 'Km (uM)' = 134; TBER1 column 'kcat (min^-1)' = 25; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.19.

normalized_value_unit_evidence: Km: printed cell 134 uM; 134 / 1000 = 0.134 mM; the exported value is 0.134 mM. kcat: printed cell 25 min^-1; the printed turnover column is per MINUTE, so 25 / 60 = 0.4166666666666667 s^(-1); the exported value is 0.4166666667 s^(-1). kcat/Km: printed cell 0.19 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.19 x 1000 / 60 = 3.1666666666666665 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Oxidation row 'S-Indan-1-ol', TBER1 column group: Km = 134 uM; kcat = 25 min^-1; printed kcat/Km = 0.19 min^-1 uM^-1.

row_label: Table 1, Oxidation section, substrate row 'S-Indan-1-ol', TBER1 column group


substrate_name: S-Camphorquinone

column_label: Table 1, Reduction section, substrate row 'S-Camphorquinone'; TBER1 column 'Km (uM)' = 9.9; TBER1 column 'kcat (min^-1)' = 113; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 11.

normalized_value_unit_evidence: Km: printed cell 9.9 uM; 9.9 / 1000 = 0.0099 mM; the exported value is 0.0099 mM. kcat: printed cell 113 min^-1; the printed turnover column is per MINUTE, so 113 / 60 = 1.8833333333333333 s^(-1); the exported value is 1.8833333333 s^(-1). kcat/Km: printed cell 11 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 11 x 1000 / 60 = 183.33333333333334 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row 'S-Camphorquinone', TBER1 column group: Km = 9.9 uM; kcat = 113 min^-1; printed kcat/Km = 11 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row 'S-Camphorquinone', TBER1 column group

substrate: S-Camphorquinone


column_label: Table 1, Reduction section, substrate row 'S-Camphorquinone'; Rat 17HSD5 column 'Km (uM)' = 5.7; Rat 17HSD5 column 'kcat (min^-1)' = 12; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 2.1.

normalized_value_unit_evidence: Km: printed cell 5.7 uM; 5.7 / 1000 = 0.0057 mM; the exported value is 0.0057 mM. kcat: printed cell 12 min^-1; the printed turnover column is per MINUTE, so 12 / 60 = 0.2 s^(-1); the exported value is 0.2 s^(-1). kcat/Km: printed cell 2.1 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 2.1 x 1000 / 60 = 35.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row 'S-Camphorquinone', Rat 17HSD5 column group: Km = 5.7 uM; kcat = 12 min^-1; printed kcat/Km = 2.1 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row 'S-Camphorquinone', Rat 17HSD5 column group


substrate_name: R-Camphorquinone

column_label: Table 1, Reduction section, substrate row 'R-Camphorquinone'; TBER1 column 'Km (uM)' = 33; TBER1 column 'kcat (min^-1)' = 78; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 2.4.

normalized_value_unit_evidence: Km: printed cell 33 uM; 33 / 1000 = 0.033 mM; the exported value is 0.033 mM. kcat: printed cell 78 min^-1; the printed turnover column is per MINUTE, so 78 / 60 = 1.3 s^(-1); the exported value is 1.3 s^(-1). kcat/Km: printed cell 2.4 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 2.4 x 1000 / 60 = 40.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row 'R-Camphorquinone', TBER1 column group: Km = 33 uM; kcat = 78 min^-1; printed kcat/Km = 2.4 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row 'R-Camphorquinone', TBER1 column group

substrate: R-Camphorquinone


column_label: Table 1, Reduction section, substrate row 'R-Camphorquinone'; Rat 17HSD5 column 'Km (uM)' = 1.6; Rat 17HSD5 column 'kcat (min^-1)' = 11; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 6.9.

normalized_value_unit_evidence: Km: printed cell 1.6 uM; 1.6 / 1000 = 0.0016 mM; the exported value is 0.0016 mM. kcat: printed cell 11 min^-1; the printed turnover column is per MINUTE, so 11 / 60 = 0.18333333333333332 s^(-1); the exported value is 0.1833333333 s^(-1). kcat/Km: printed cell 6.9 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 6.9 x 1000 / 60 = 115.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 1, Table 1 Reduction row 'R-Camphorquinone', Rat 17HSD5 column group: Km = 1.6 uM; kcat = 11 min^-1; printed kcat/Km = 6.9 min^-1 uM^-1.

row_label: Table 1, Reduction section, substrate row 'R-Camphorquinone', Rat 17HSD5 column group


column_label: Table 2, (A) section, substrate row '5b-Androstan-17b-ol-3-one'; Rat 17HSD5 column 'Km (uM)' = 31; Rat 17HSD5 column 'kcat (min^-1)' = 22; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.71.

normalized_value_unit_evidence: Km: printed cell 31 uM; 31 / 1000 = 0.031 mM; the exported value is 0.031 mM. kcat: printed cell 22 min^-1; the printed turnover column is per MINUTE, so 22 / 60 = 0.36666666666666664 s^(-1); the exported value is 0.3666666667 s^(-1). kcat/Km: printed cell 0.71 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.71 x 1000 / 60 = 11.833333333333334 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (A) row '5b-Androstan-17b-ol-3-one', Rat 17HSD5 column group: Km = 31 uM; kcat = 22 min^-1; printed kcat/Km = 0.71 min^-1 uM^-1.

row_label: Table 2, (A) section, substrate row '5b-Androstan-17b-ol-3-one', Rat 17HSD5 column group


column_label: Table 2, (B) section, substrate row '4-Pregenene-17a,20a-diol-3-one'; TBER1 column 'Km (uM)' = 12; TBER1 column 'kcat (min^-1)' = 7.9; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.66.

normalized_value_unit_evidence: Km: printed cell 12 uM; 12 / 1000 = 0.012 mM; the exported value is 0.012 mM. kcat: printed cell 7.9 min^-1; the printed turnover column is per MINUTE, so 7.9 / 60 = 0.13166666666666668 s^(-1); the exported value is 0.1316666667 s^(-1). kcat/Km: printed cell 0.66 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.66 x 1000 / 60 = 11.0 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (B) row '4-Pregenene-17a,20a-diol-3-one', TBER1 column group: Km = 12 uM; kcat = 7.9 min^-1; printed kcat/Km = 0.66 min^-1 uM^-1.

row_label: Table 2, (B) section, substrate row '4-Pregenene-17a,20a-diol-3-one', TBER1 column group


column_label: Table 2, (B) section, substrate row '4-Pregenen-20a-ol-3-one'; TBER1 column 'Km (uM)' = 8.1; TBER1 column 'kcat (min^-1)' = 3.2; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.39.

normalized_value_unit_evidence: Km: printed cell 8.1 uM; 8.1 / 1000 = 0.0081 mM; the exported value is 0.0081 mM. kcat: printed cell 3.2 min^-1; the printed turnover column is per MINUTE, so 3.2 / 60 = 0.05333333333333334 s^(-1); the exported value is 0.0533333333 s^(-1). kcat/Km: printed cell 0.39 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.39 x 1000 / 60 = 6.5 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (B) row '4-Pregenen-20a-ol-3-one', TBER1 column group: Km = 8.1 uM; kcat = 3.2 min^-1; printed kcat/Km = 0.39 min^-1 uM^-1.

row_label: Table 2, (B) section, substrate row '4-Pregenen-20a-ol-3-one', TBER1 column group


column_label: Table 2, (B) section, substrate row '4-Pregenen-20a-ol-3-one'; Rat 17HSD5 column 'Km (uM)' = 24; Rat 17HSD5 column 'kcat (min^-1)' = 8.5; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.35.

normalized_value_unit_evidence: Km: printed cell 24 uM; 24 / 1000 = 0.024 mM; the exported value is 0.024 mM. kcat: printed cell 8.5 min^-1; the printed turnover column is per MINUTE, so 8.5 / 60 = 0.14166666666666666 s^(-1); the exported value is 0.1416666667 s^(-1). kcat/Km: printed cell 0.35 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.35 x 1000 / 60 = 5.833333333333333 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (B) row '4-Pregenen-20a-ol-3-one', Rat 17HSD5 column group: Km = 24 uM; kcat = 8.5 min^-1; printed kcat/Km = 0.35 min^-1 uM^-1.

row_label: Table 2, (B) section, substrate row '4-Pregenen-20a-ol-3-one', Rat 17HSD5 column group


column_label: Table 2, (B) section, substrate row '4-Androsten-3a-ol-17-one'; TBER1 column 'Km (uM)' = 14; TBER1 column 'kcat (min^-1)' = 3.6; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.25.

normalized_value_unit_evidence: Km: printed cell 14 uM; 14 / 1000 = 0.014 mM; the exported value is 0.014 mM. kcat: printed cell 3.6 min^-1; the printed turnover column is per MINUTE, so 3.6 / 60 = 0.060000000000000005 s^(-1); the exported value is 0.06 s^(-1). kcat/Km: printed cell 0.25 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.25 x 1000 / 60 = 4.166666666666667 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (B) row '4-Androsten-3a-ol-17-one', TBER1 column group: Km = 14 uM; kcat = 3.6 min^-1; printed kcat/Km = 0.25 min^-1 uM^-1.

row_label: Table 2, (B) section, substrate row '4-Androsten-3a-ol-17-one', TBER1 column group


column_label: Table 2, (C) section, substrate row '4-Androsten-3a-ol-17-one'; TBER1 column 'Km (uM)' = 13; TBER1 column 'kcat (min^-1)' = 1.3; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.10.

normalized_value_unit_evidence: Km: printed cell 13 uM; 13 / 1000 = 0.013 mM; the exported value is 0.013 mM. kcat: printed cell 1.3 min^-1; the printed turnover column is per MINUTE, so 1.3 / 60 = 0.021666666666666667 s^(-1); the exported value is 0.0216666667 s^(-1). kcat/Km: printed cell 0.10 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.10 x 1000 / 60 = 1.6666666666666667 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (C) row '4-Androsten-3a-ol-17-one', TBER1 column group: Km = 13 uM; kcat = 1.3 min^-1; printed kcat/Km = 0.10 min^-1 uM^-1.

row_label: Table 2, (C) section, substrate row '4-Androsten-3a-ol-17-one', TBER1 column group


column_label: Table 2, (C) section, substrate row '4-Androsten-3a-ol-17-one'; Rat 17HSD5 column 'Km (uM)' = 8.5; Rat 17HSD5 column 'kcat (min^-1)' = 1.9; Rat 17HSD5 column 'kcat/Km (min^-1 uM^-1)' = 0.22.

normalized_value_unit_evidence: Km: printed cell 8.5 uM; 8.5 / 1000 = 0.0085 mM; the exported value is 0.0085 mM. kcat: printed cell 1.9 min^-1; the printed turnover column is per MINUTE, so 1.9 / 60 = 0.03166666666666666 s^(-1); the exported value is 0.0316666667 s^(-1). kcat/Km: printed cell 0.22 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.22 x 1000 / 60 = 3.6666666666666665 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (C) row '4-Androsten-3a-ol-17-one', Rat 17HSD5 column group: Km = 8.5 uM; kcat = 1.9 min^-1; printed kcat/Km = 0.22 min^-1 uM^-1.

row_label: Table 2, (C) section, substrate row '4-Androsten-3a-ol-17-one', Rat 17HSD5 column group


column_label: Table 2, (D) section, substrate row '5b-Pregnan-20a-ol-3-one'; TBER1 column 'Km (uM)' = 19; TBER1 column 'kcat (min^-1)' = 1.0; TBER1 column 'kcat/Km (min^-1 uM^-1)' = 0.05.

normalized_value_unit_evidence: Km: printed cell 19 uM; 19 / 1000 = 0.019 mM; the exported value is 0.019 mM. kcat: printed cell 1.0 min^-1; the printed turnover column is per MINUTE, so 1.0 / 60 = 0.016666666666666666 s^(-1); the exported value is 0.0166666667 s^(-1). kcat/Km: printed cell 0.05 min^-1 uM^-1; 1 min^-1 uM^-1 is 1000 / 60 s^(-1)*mM^(-1), so 0.05 x 1000 / 60 = 0.8333333333333334 s^(-1)*mM^(-1); this catalog row carries no catalytic-efficiency value, so the printed cell enters this record as paper-side evidence only.

raw_value_unit_evidence: Table 2, Table 2 (D) row '5b-Pregnan-20a-ol-3-one', TBER1 column group: Km = 19 uM; kcat = 1.0 min^-1; printed kcat/Km = 0.05 min^-1 uM^-1.

row_label: Table 2, (D) section, substrate row '5b-Pregnan-20a-ol-3-one', TBER1 column group


organism: Brucella melitensis biotype 1


organism: Aeromonas hydrophila

substrate_name: Carbenicillin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Carbenicillin' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 450 +/- 30; column 'kcat (s-1)' = 16.00; column 'kcat/Km (M-1 s-1)' = 3.60 x 10^4.

normalized_value_unit_evidence: Km: printed Table 3 cell 450 uM; 450 / 1000 = 0.45 mM; the exported value is 0.45 mM. kcat: printed Table 3 cell 16.00 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 16 s^(-1). kcat/Km: printed Table 3 cell 3.60 x 10^4 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 36000 / 1000 = 36 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Carbenicillin': Km = 450 +/- 30 uM; kcat = 16.00 s^-1; printed kcat/Km = 3.60 x 10^4 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Carbenicillin' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Carbenicillin


substrate_name: Penicillin G

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Penicillin G' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 700 +/- 50; column 'kcat (s-1)' = 5.21; column 'kcat/Km (M-1 s-1)' = 7.40 x 10^3.

normalized_value_unit_evidence: Km: printed Table 3 cell 700 uM; 700 / 1000 = 0.7 mM; the exported value is 0.7 mM. kcat: printed Table 3 cell 5.21 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 5.21 s^(-1). kcat/Km: printed Table 3 cell 7.40 x 10^3 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 7400 / 1000 = 7.4 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Penicillin G': Km = 700 +/- 50 uM; kcat = 5.21 s^-1; printed kcat/Km = 7.40 x 10^3 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Penicillin G' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Penicillin G


substrate_name: Oxacillin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Oxacillin' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 25 +/- 3; column 'kcat (s-1)' = 0.75; column 'kcat/Km (M-1 s-1)' = 3.00 x 10^4.

normalized_value_unit_evidence: Km: printed Table 3 cell 25 uM; 25 / 1000 = 0.025 mM; the exported value is 0.025 mM. kcat: printed Table 3 cell 0.75 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.75 s^(-1). kcat/Km: printed Table 3 cell 3.00 x 10^4 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 30000 / 1000 = 30 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Oxacillin': Km = 25 +/- 3 uM; kcat = 0.75 s^-1; printed kcat/Km = 3.00 x 10^4 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Oxacillin' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Oxacillin


substrate_name: Cephaloridin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cephaloridin' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 190 +/- 8; column 'kcat (s-1)' = 0.12; column 'kcat/Km (M-1 s-1)' = 6.30 x 10^2.

normalized_value_unit_evidence: Km: printed Table 3 cell 190 uM; 190 / 1000 = 0.19 mM; the exported value is 0.19 mM. kcat: printed Table 3 cell 0.12 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.12 s^(-1). kcat/Km: printed Table 3 cell 6.30 x 10^2 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 630 / 1000 = 0.63 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Cephaloridin': Km = 190 +/- 8 uM; kcat = 0.12 s^-1; printed kcat/Km = 6.30 x 10^2 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cephaloridin' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Cephaloridin


substrate_name: Cephaloglycin

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cephaloglycin' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 280 +/- 30; column 'kcat (s-1)' = 0.20; column 'kcat/Km (M-1 s-1)' = 7.10 x 10^2.

normalized_value_unit_evidence: Km: printed Table 3 cell 280 uM; 280 / 1000 = 0.28 mM; the exported value is 0.28 mM. kcat: printed Table 3 cell 0.20 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.2 s^(-1). kcat/Km: printed Table 3 cell 7.10 x 10^2 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 710 / 1000 = 0.71 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Cephaloglycin': Km = 280 +/- 30 uM; kcat = 0.20 s^-1; printed kcat/Km = 7.10 x 10^2 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cephaloglycin' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Cephaloglycin


substrate_name: Cefuroxime

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cefuroxime' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 20 +/- 3; column 'kcat (s-1)' = 0.07; column 'kcat/Km (M-1 s-1)' = 3.50 x 10^3.

normalized_value_unit_evidence: Km: printed Table 3 cell 20 uM; 20 / 1000 = 0.02 mM; the exported value is 0.02 mM. kcat: printed Table 3 cell 0.07 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.07 s^(-1). kcat/Km: printed Table 3 cell 3.50 x 10^3 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 3500 / 1000 = 3.5 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Cefuroxime': Km = 20 +/- 3 uM; kcat = 0.07 s^-1; printed kcat/Km = 3.50 x 10^3 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cefuroxime' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Cefuroxime


substrate_name: Cefotaxime

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Cefotaxime' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 26 +/- 3; column 'kcat (s-1)' = 0.07; column 'kcat/Km (M-1 s-1)' = 2.70 x 10^3.

normalized_value_unit_evidence: Km: printed Table 3 cell 26 uM; 26 / 1000 = 0.026 mM; the exported value is 0.026 mM. kcat: printed Table 3 cell 0.07 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.07 s^(-1). kcat/Km: printed Table 3 cell 2.70 x 10^3 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 2700 / 1000 = 2.7 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Cefotaxime': Km = 26 +/- 3 uM; kcat = 0.07 s^-1; printed kcat/Km = 2.70 x 10^3 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Cefotaxime' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Cefotaxime


substrate_name: Imipenem

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Imipenem' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 80 +/- 8; column 'kcat (s-1)' = 168.00; column 'kcat/Km (M-1 s-1)' = 2.10 x 10^6.

normalized_value_unit_evidence: Km: printed Table 3 cell 80 uM; 80 / 1000 = 0.08 mM; the exported value is 0.08 mM. kcat: printed Table 3 cell 168.00 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 168 s^(-1). kcat/Km: printed Table 3 cell 2.10 x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 2100000 / 1000 = 2100 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Imipenem': Km = 80 +/- 8 uM; kcat = 168.00 s^-1; printed kcat/Km = 2.10 x 10^6 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Imipenem' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Imipenem


column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row '6-beta-IP' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 530 +/- 50; column 'kcat (s-1)' = 0.64; column 'kcat/Km (M-1 s-1)' = 1.20 x 10^3.

normalized_value_unit_evidence: Km: printed Table 3 cell 530 uM; 530 / 1000 = 0.53 mM; the exported value is 0.53 mM. kcat: printed Table 3 cell 0.64 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.64 s^(-1). kcat/Km: printed Table 3 cell 1.20 x 10^3 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1200 / 1000 = 1.2 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row '6-beta-IP': Km = 530 +/- 50 uM; kcat = 0.64 s^-1; printed kcat/Km = 1.20 x 10^3 M^-1 s^-1.

row_label: Table 3, antibiotic row '6-beta-IP' within the 'Aeromonas hydrophila A2 enzyme' column group


substrate_name: Sulbactam

column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row 'Sulbactam' within the 'Aeromonas hydrophila A2 enzyme' column group; column 'Km (uM)' = 37 +/- 4; column 'kcat (s-1)' = 0.12; column 'kcat/Km (M-1 s-1)' = 3.24 x 10^3.

normalized_value_unit_evidence: Km: printed Table 3 cell 37 uM; 37 / 1000 = 0.037 mM; the exported value is 0.037 mM. kcat: printed Table 3 cell 0.12 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 0.12 s^(-1). kcat/Km: printed Table 3 cell 3.24 x 10^3 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 3240 / 1000 = 3.24 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Aeromonas hydrophila A2 enzyme' column group, antibiotic row 'Sulbactam': Km = 37 +/- 4 uM; kcat = 0.12 s^-1; printed kcat/Km = 3.24 x 10^3 M^-1 s^-1.

row_label: Table 3, antibiotic row 'Sulbactam' within the 'Aeromonas hydrophila A2 enzyme' column group

substrate: Sulbactam


column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row '6-beta-IP' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group; column 'Km (uM)' = 640 +/- 30; column 'kcat (s-1)' = 648; column 'kcat/Km (M-1 s-1)' = 1.00 x 10^6.

normalized_value_unit_evidence: Km: printed Table 3 cell 640 uM; 640 / 1000 = 0.64 mM; the exported value is 0.64 mM. kcat: printed Table 3 cell 648 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 648 s^(-1). kcat/Km: printed Table 3 cell 1.00 x 10^6 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1000000 / 1000 = 1000 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group, antibiotic row '6-beta-IP': Km = 640 +/- 30 uM; kcat = 648 s^-1; printed kcat/Km = 1.00 x 10^6 M^-1 s^-1.

row_label: Table 3, antibiotic row '6-beta-IP' within the 'Pseudomonas maltophilia ULA-511 L-1 enzyme' column group


column_label: Table 3 (Kinetic parameters determined for class B beta-lactamases), antibiotic row '6-beta-IP' within the 'Bacillus cereus beta-lactamase II' column group; column 'Km (uM)' = 7460 +/- 590; column 'kcat (s-1)' = 450; column 'kcat/Km (M-1 s-1)' = 6.00 x 10^4.

normalized_value_unit_evidence: Km: printed Table 3 cell 7460 uM; 7460 / 1000 = 7.46 mM; the exported value is 7.46 mM. kcat: printed Table 3 cell 450 s^-1; the cell is already in s^-1, so no unit conversion applies here; the exported value is 450 s^(-1). kcat/Km: printed Table 3 cell 6.00 x 10^4 M^-1 s^-1; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 60000 / 1000 = 60 s^-1 mM^-1; the catalog identity keeps no catalytic-efficiency value, so this printed cell is carried here as typed provenance only.

raw_value_unit_evidence: Table 3, 'Bacillus cereus beta-lactamase II' column group, antibiotic row '6-beta-IP': Km = 7460 +/- 590 uM; kcat = 450 s^-1; printed kcat/Km = 6.00 x 10^4 M^-1 s^-1.

row_label: Table 3, antibiotic row '6-beta-IP' within the 'Bacillus cereus beta-lactamase II' column group


enzyme_display_name: alcohol dehydrogenase

organism: Thermococcus kodakarensis

enzyme: alcohol dehydrogenase