sequence_source: UniProt REST A0A0A1EQY0 canonical sequence retained as accession context only

proof_lines: wild type enzyme R174N, at pH 7.5 and 25°C


enzyme_display_name: formate dehydrogenase

organism: [Candida] boidinii

substrate_name: NAD+

source_db: brenda

kcat_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

protein_accession_database: UniProt

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: Resolved canonical sequence from UniProt REST A0A0A1EQY0 sequence plus row-specific BRENDA mutation note and applied mutation signature R174N to reconstruct the assayed variant sequence.

mutation_type: substitution

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NAD+

enzyme: formate dehydrogenase

substrate: NAD+

source: brenda


organism: [Candida] methylica

substrate_name: formate

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

substrate: formate


proof_lines: mutant enzyme N187E/N147R, at pH 8.0 and 25°C


km_display: Not reported

mutation_signature: Unknown

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


proof_lines: mutant enzyme N187E/Q105R, at pH 8.0 and 25°C


sequence_variant_note: Resolved canonical sequence from NCBI Protein CAA57036.1; NAD-dependent formate dehydrogenase [[Candida] methylica]; reconstructed from selected mutation_signature and applied mutation signature T226V to reconstruct the assayed variant sequence.


sequence_source: NCBI Protein CAA57036.1; NAD-dependent formate dehydrogenase [[Candida] methylica]; canonical context only, variant not reconstructed

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.


sequence_variant_note: Resolved canonical sequence from NCBI Protein CAA57036.1; NAD-dependent formate dehydrogenase [[Candida] methylica]; reconstructed from selected mutation_signature and applied mutation signature T169C/T226C to reconstruct the assayed variant sequence.

proof_lines: oxidized mutant enzyme T169C/T226C, at 20°C, in 20 mM triethanolamine at pH 8

proof_lines: reduced mutant enzyme T169C/T226C, at 20°C, in 20 mM triethanolamine at pH 8


substrate_name: NADP+

sequence_variant_note: Resolved canonical sequence from NCBI Protein CAA57036.1; NAD-dependent formate dehydrogenase [[Candida] methylica]; reconstructed from selected mutation_signature and applied mutation signature D195S/Y196A to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 8; 298.15 K; cofactor NADP+

proof_lines: mutant enzyme D195S/Y196A, at pH 8.0 and 25°C

substrate: NADP+


sequence_variant_note: Resolved canonical sequence from NCBI Protein CAA57036.1; NAD-dependent formate dehydrogenase [[Candida] methylica]; reconstructed from selected mutation_signature and applied mutation signature D195S/Q197V to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme D195S/Q197V, at pH 8.0 and 25°C


sequence_variant_note: Resolved canonical sequence from NCBI Protein CAA57036.1; NAD-dependent formate dehydrogenase [[Candida] methylica]; reconstructed from selected mutation_signature and applied mutation signature D195S/Y196L to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme D195S/Y196L, at pH 8.0 and 25°C


sequence_variant_note: Resolved canonical sequence from NCBI Protein CAA57036.1; NAD-dependent formate dehydrogenase [[Candida] methylica]; reconstructed from selected mutation_signature and applied mutation signature D195S/Y196S to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme D195S/Y196S, at pH 8.0 and 25°C


sequence_variant_note: Resolved canonical sequence from NCBI Protein CAA57036.1; NAD-dependent formate dehydrogenase [[Candida] methylica]; reconstructed from selected mutation_signature and applied mutation signature D195S to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 8; 293.15 K; cofactor NADP+

proof_lines:
pH 8.0, 20°C, mutant enzyme D195S
mutant enzyme D195S, at pH 8.0 and 25°C
mutant enzyme D195S


sequence_variant_note: Resolved canonical sequence from NCBI Protein CAA57036.1; NAD-dependent formate dehydrogenase [[Candida] methylica]; reconstructed from selected mutation_signature and applied mutation signature Q197V to reconstruct the assayed variant sequence.


sequence_source: NCBI Protein CAA57036.1; NAD-dependent formate dehydrogenase [[Candida] methylica]

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

assay_conditions_summary: pH 8; 298.15 K; cofactor NAD+

proof_lines:
wild type enzyme, at pH 8.0 and 25°C
wild-type enzyme
pH 8.0, 20°C, wild-type enzyme


sequence_variant_note: Resolved canonical sequence from NCBI Protein CAA57036.1; NAD-dependent formate dehydrogenase [[Candida] methylica]; reconstructed from selected mutation_signature and applied mutation signature D195S/Q197T to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme D195S/Q197T, at pH 8.0 and 25°C


sequence_source: NCBI Protein CAA57036.1; NAD-dependent formate dehydrogenase [[Candida] methylica]; context only, not accepted as row-specific sequence

assay_conditions_summary: pH 7; 298.15 K; cofactor NAD+

proof_lines: with HCO3- as cosubstrate, pH 8.0, temperature not specified in the publication

proof_lines: in the presence of 0.003 mM Fe3+, at pH 8.0 and 25°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:O13437 canonical sequence; Q00498 is a secondary accession; row-specific BRENDA mutation note N147R and applied mutation signature N147R to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:O13437 canonical sequence; Q00498 is a secondary accession; row-specific BRENDA mutation note Y302R and applied mutation signature Y302R to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:O13437 canonical sequence; Q00498 is a secondary accession; row-specific BRENDA mutation note H13E and applied mutation signature H13E to reconstruct the assayed variant sequence.


organism: Clostridium carboxidivorans

substrate_name: NADH

sequence_source: UniProtKB:E2IQB0 direct accession lookup

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

assay_conditions_summary: pH 6.8; 310.15 K; cofactor NADH

proof_lines: in 0.1 M sodium phosphate (pH 6.8), at 37°C

substrate: NADH


organism: Clostridium ljungdahlii

assay_conditions_summary: pH 9; 298.15 K; cofactor NAD+


organism: Gelatoporia subvermispora

sequence_source: UniProtKB:A9ZNT9 direct accession lookup

assay_conditions_summary: cofactor NAD+


sequence_source: UniProtKB:A9ZNT8 direct accession lookup

assay_conditions_summary: pH 6.5; 298.15 K; cofactor NAD+

proof_lines: in the absence of metal ion, at pH 6.5 and 25°C

proof_lines: in the presence of 0.005 mM Mn2+, at pH 6.5 and 25°C


organism: Moraxella sp.

assay_conditions_summary: pH 5.5; 298.15 K; buffer 0.1 M potassium phosphate; cofactor NAD+

proof_lines: in the presence of 0.005 mM Na+, at pH 5.5 and 25°C

proof_lines: in the absence of metal ion, at pH 5.5 and 25°C

proof_lines: in 0.1 M potassium phosphate buffer pH 7.0, at 30°C


organism: Moraxella sp. C-1

proof_lines: wild type enzyme, pH and temperature not specified in the publication


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: mutant enzyme A198G, pH and temperature not specified in the publication


organism: Paracoccus sp. 12-A

sequence_source: UniProtKB:Q93GW3 direct accession lookup

proof_lines: in the presence of 0.005 mM Mg2+, at pH 6.5 and 25°C


organism: Pseudomonas sp. 101

sequence_source: UniProtKB:P33160 canonical sequence context only

proof_lines: mutant enzyme A198G/D221S, pH and temperature not specified in the publication


assay_conditions_summary: cofactor NADP+

proof_lines: mutant enzyme D221S, pH and temperature not specified in the publication


sequence_source: UniProtKB REST direct accession check on 2026-06-19


organism: Saccharomyces cerevisiae

assay_conditions_summary: pH 7; 303.15 K; cofactor NADP+

proof_lines: pH 7.0, 30°C, mutant enzyme D196A/Y197R, with 0.5 M formate

proof_lines: pH 7.0, 30°C, mutant enzyme D196A/Y197R, with 0.25 M formate


assay_conditions_summary: pH 7; 303.15 K; cofactor NAD+


proof_lines: in the presence of 0.007 mM Fe3+, at pH 6.5 and 25°C


organism: Thermochaetoides thermophila

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

assay_conditions_summary: pH 7.4; 298.15 K; cofactor NAD+

proof_lines: C-terminally His-tagged enzyme, at pH 5.0 and 25°C

proof_lines: N-terminally His-tagged enzyme, at pH 5.0 and 25°C


organism: Thermothelomyces thermophilus

assay_conditions_summary: pH 10.5; 298.15 K; cofactor NAD+

proof_lines: 25°C, pH not specified in the publication


organism: Thiobacillus sp. KNK65MA

proof_lines: in the presence of 0.007 mM W6+, at pH 7.0 and 25°C

proof_lines: in the absence of metal ion, at pH 7.0 and 25°C


organism: Ancylobacter aquaticus

sequence_source: UniProtKB Q845T0 direct REST lookup; live TrEMBL formate dehydrogenase entry for Ancylobacter aquaticus with EC 1.17.1.9.

assay_conditions_summary: pH 6; 298.15 K; cofactor NAD+

proof_lines: in the presence of 0.003 mM Fe3+, at pH 6.0 and 25°C

proof_lines: in the absence of metal ion, at pH 6.0 and 25°C


organism: Bacillus sp. (in: firmicutes)

proof_lines: with NAD+ as cosubstrate, in 100 mM Tris-HCl (pH 7.0), at 30°C

proof_lines: with NADP+ as cosubstrate, in 100 mM Tris-HCl (pH 7.0), at 30°C


sequence_variant_note: Resolved canonical sequence from UniProtKB O13437 canonical sequence with row-specific reconstruction of K47E. and applied mutation signature K47E to reconstruct the assayed variant sequence.

assay_conditions_summary: 293.15 K; cofactor NAD+

proof_lines: mutant enzyme K47E, at 20°C, after 2 weeks of enzyme storage

proof_lines: mutant enzyme K47E, at 20°C, after 4 months of enzyme storage


sequence_variant_note: Resolved canonical sequence from UniProtKB O13437 canonical sequence with row-specific reconstruction of D195A. and applied mutation signature D195A to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 7.5; 310.15 K; buffer 0.1 M potassium phosphate; cofactor NADP+

proof_lines: mutant enzyme D195A, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB O13437 canonical sequence with row-specific reconstruction of D195Q/Y196P. and applied mutation signature D195Q/Y196P to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme D195Q/Y196P, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB O13437 canonical sequence with row-specific reconstruction of D195Q/Y196H. and applied mutation signature D195Q/Y196H to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme D195Q/Y196H, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB O13437 canonical sequence with row-specific reconstruction of D195Q. and applied mutation signature D195Q to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme D195Q, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB O13437 canonical sequence with row-specific reconstruction of D195N. and applied mutation signature D195N to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme D195N, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB O13437 canonical sequence with row-specific reconstruction of D195Q/Y196S. and applied mutation signature D195Q/Y196S to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme D195Q/Y196S, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C


sequence_source: UniProtKB O13437 direct REST lookup; reviewed Swiss-Prot Candida boidinii formate dehydrogenase with EC 1.17.1.9.

proof_lines: wild type enzyme, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB O13437 canonical sequence with row-specific reconstruction of D195S. and applied mutation signature D195S to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme D195S, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C


sequence_variant_note: Resolved canonical sequence from UniProtKB O13437 canonical sequence with row-specific reconstruction of N187S/T321S. and applied mutation signature N187S/T321S to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme N187S/T321S, at pH 7.2 and 30°C


sequence_variant_note: Resolved canonical sequence from UniProtKB O13437 canonical sequence with row-specific reconstruction of T321S. and applied mutation signature T321S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB O13437 canonical sequence with row-specific reconstruction of N187S. and applied mutation signature N187S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB O13437 canonical sequence with row-specific reconstruction of K328V. and applied mutation signature K328V to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NAD+

proof_lines: mutant enzyme K328V, at 20°C, after 2 weeks of enzyme storage

proof_lines: mutant enzyme K328V, at 20°C, after 4 months of enzyme storage


sequence_variant_note: Resolved canonical sequence from UniProtKB O13437 canonical sequence with row-specific reconstruction of N187D. and applied mutation signature N187D to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 9.5; 303.15 K; cofactor NAD+


organism: Gossypium hirsutum

proof_lines:
at pH 7.0 and 30°C
native enzyme, at pH 7.0 and 25°C


proof_lines: mutant enzyme M225L/M234L/M243L, at pH 7.0 and 25°C


proof_lines: mutant enzyme M225L/M243L, at pH 7.0 and 25°C


organism: Gottschalkia acidurici

substrate_name: benzyl viologen

substrate: benzyl viologen


organism: Kloeckera sp.


organism: Komagataella pastoris


organism: Lathyrus oleraceus

substrate_name: S-formylglutathione

proof_lines: and 1.67 mM at the second catalytic site

proof_lines: and 6.25 mM at the second catalytic site

substrate: S-formylglutathione


organism: Methylobacterium sp.


organism: Mycolicibacterium vaccae

assay_conditions_summary: pH 7; 295.15 K; buffer 0.1 M sodium phosphate; cofactor NADP+

proof_lines: mutant enzyme D221G, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C


proof_lines: mutant enzyme C145S/D221G/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C


organism: Ogataea parapolymorpha

proof_lines: pH and temperature not specified in the publication


organism: Paracoccus sp. (in: a-proteobacteria)

substrate_name: glyoxylate

substrate: glyoxylate


organism: Pseudomonas sp.


assay_conditions_summary: pH 7; 310.15 K; buffer 0.1 M sodium phosphate; cofactor NAD+

proof_lines: wild type enzyme, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


assay_conditions_summary: pH 7; buffer 0.1 M sodium phosphate; cofactor NAD+

proof_lines: mutant enzyme F311S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme F311Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


organism: [Candida] methylica

assay_conditions_summary: pH 8; 298.15 K; cofactor NAD+


assay_conditions_summary: cofactor NAD+


organism: Citrobacter sp.


organism: Cupriavidus necator


organism: Cupriavidus oxalaticus

proof_lines: with HCO3- as cosubstrate, pH and temperature not specified in the publication

proof_lines: formate dehydrogenase II, reaction with formate


organism: Desulfovibrio desulfuricans

proof_lines:
at pH 7.0 and 22°C
at pH 8.0 and 22°C


organism: Gelatoporia subvermispora

assay_conditions_summary: pH 6.5; 303.15 K; cofactor NAD+

proof_lines:
pH 6.5, 30°C
substrate NAD+, pH 6.5, 30°C
substrate formate, pH 6.5, 30°C


organism: Glycine max

assay_conditions_summary: pH 7; 303.15 K; buffer 0.1 M sodium phosphate; cofactor NAD+

proof_lines: mutant enzyme F290N, in 0.1 M sodium phosphate buffer, pH 7.0, at 30°C

proof_lines: mutant enzyme F290N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme F290D, in 0.1 M sodium phosphate buffer, pH 7.0, at 30°C

proof_lines: mutant enzyme F290D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme F290S, in 0.1 M sodium phosphate buffer, pH 7.0, at 30°C

proof_lines: mutant enzyme F290S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


proof_lines: wild type enzyme, in 0.1 M sodium phosphate buffer, pH 7.0, at 30°C


assay_conditions_summary: pH 8; 310.15 K; cofactor NAD+


proof_lines: mutant enzyme A267M/I272V/F290S, at pH 8.0 and 37°C


proof_lines: mutant enzyme A267M/I272V/F290N, at pH 8.0 and 37°C


proof_lines: mutant enzyme A267M/I272V/F290D, at pH 8.0 and 37°C


proof_lines: mutant enzyme A267M/I272V, at pH 8.0 and 37°C


assay_conditions_summary: pH 7; buffer 0.1 M sodium phosphate

proof_lines: mutant enzyme F290Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme F290T, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme F290Q, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme F290E, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme F290A, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


organism: Glycine soja


organism: Saccharomyces cerevisiae

substrate_name: thio-NAD+

proof_lines: with 3-pyridinecarboxaldehyde-NAD+ as electron acceptor

substrate: thio-NAD+


organism: Thermochaetoides thermophila

proof_lines: wild type enzyme, with HCO3- as cosubstrate, at pH 5.0, temperature not specified in the publication


proof_lines: mutant enzyme N256D, at pH 8.0, temperature not specified in the publication


assay_conditions_summary: pH 7; 298.15 K; cofactor NAD+

proof_lines: mutant enzyme N120C, at pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme G93H/I94Y, at pH 7.0 and 25°C


proof_lines: mutant enzyme R259C, at pH 8.0, temperature not specified in the publication


proof_lines: mutant enzyme G93H/I94Y/R259C, at pH 8.0, temperature not specified in the publication

proof_lines: mutant enzyme G93H/I94Y/R259C, at pH 7.0 and 25°C


assay_conditions_summary: pH 8; cofactor NAD+

proof_lines: mutant enzyme G193H/I94Y, at pH 8.0, temperature not specified in the publication


proof_lines: mutant enzyme G93H/I94R, at pH 8.0, temperature not specified in the publication

proof_lines: mutant enzyme G93H/I94R, at pH 7.0 and 25°C


organism: Methylosinus trichosporium

substrate_name: FMN

substrate: FMN


organism: methylotrophic bacterium


proof_lines: mutant enzyme C145S/A198G/D221Q/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C

proof_lines: mutant enzyme C145S/A198G/D221Q/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 30°C


proof_lines: Km above 0.04 mM, wild type enzyme, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C


proof_lines: mutant enzyme D221Q, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C


proof_lines: mutant enzyme D221G/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C


proof_lines: mutant enzyme C145S/D221A/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C


proof_lines: mutant enzyme C145S/D221Q/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C

proof_lines: mutant enzyme C145S/D221Q/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 30°C


proof_lines: mutant enzyme D221S, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C


proof_lines: mutant enzyme A198G/D221Q, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C


proof_lines: mutant enzyme D221A, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C


assay_conditions_summary: pH 7; buffer phosphate/EDTA; cofactor NAD+

proof_lines: mutant enzyme F311N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


proof_lines: mutant enzyme F311D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication


proof_lines:
in presence of NAD+
in presence of formate


organism: Rhodobacter capsulatus

assay_conditions_summary: pH 9; 303.15 K; cofactor NAD+

proof_lines: with NADH as cosubstrate, at pH 9.0 and 30°C

proof_lines: with 2,6-dichlorophenolindophenol as cosubstrate, at pH 9.0 and 30°C

proof_lines: with NAD+ as cosubstrate, at pH 9.0 and 30°C


organism: Rhodopseudomonas palustris


organism: Thiobacillus sp. KNK65MA

sequence_source: UniProtKB Q76EB7

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: with HCO3- as cosubstrate, pH 6.5, temperature not specified in the publication

proof_lines: at pH 5.5, temperature not specified in the publication

proof_lines: with HCO3- as cosubstrate, at pH 6.5, temperature not specified in the publication


organism: Xanthobacter sp. 91

protein_accession_database: NCBI Protein

sequence_source: NCBI RefSeq Protein WP_029558741.1

assay_conditions_summary: pH 7; 298.15 K; cofactor NADP+

proof_lines: with NADP+ as cosubstrate, at pH 7.0 and 25°C

proof_lines: with NAD+ as cosubstrate, at pH 7.0 and 25°C


enzyme_display_name: hepacivirin

organism: Unknown

substrate_name: Unknown

ki_unit: mM

enzyme: hepacivirin

substrate: Unknown


organism: Hepacivirus hominis

substrate_name: EAGNNIVPCSMSYTWTGA-OH

assay_conditions_summary: pH 7.5; 296.15 K; buffer 50 mM HEPES

proof_lines: wild-type NS3 protease, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT

proof_lines: wild-type or engineered single-chain protease plus cofactor NS4A, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT, 150 mM NaCl

substrate: EAGNNIVPCSMSYTWTGA-OH


substrate_name: EAGDDIVPCSMSYTWTGA-OH

substrate: EAGDDIVPCSMSYTWTGA-OH


proof_lines:
pH 7.5, mutant enzyme R155S
mutant R155S


proof_lines:
pH 7.5, mutant enzyme K136M
mutant enzyme K136M


proof_lines: pH 6.5, 30°C, strain 1a, mutant enzyme R123T


proof_lines: pH 6.5, 30°C, strain 3a, mutant enzyme T123R


proof_lines: pH 6.5, 30°C, strain 3a, mutant enzyme Q168D


proof_lines: mutant full-length protease M485A/V524A/Q562A, at 23°C


substrate_name: Ac-EMEECASHLP

assay_conditions_summary: pH 5.8; 296.15 K; buffer 50 mM HEPES

proof_lines: operating at wavelengths 524 nm/600 nm (excitation/emission)

proof_lines: enzyme from hepatitis C virus genotype 4a, in 1 M MES (pH 5.8), at 23°C

substrate: Ac-EMEECASHLP


organism: hepatitis C virus genotype 1a


organism: Hepatitis C virus subtype 3a

proof_lines: pH and temperature not specified in the publication


organism: West Nile virus


substrate_name: Ac-Leu-Lys-Lys-Arg-p-nitroanilide

substrate: Ac-Leu-Lys-Lys-Arg-p-nitroanilide


proof_lines: pH 6.5, 30°C, strain 3a, mutant enzyme T123R/Q168D


proof_lines: pH 6.5, 30°C, strain 3a, mutant enzyme T12 3R


proof_lines: pH 6.5, 30°C, strain 1a, mutant enzyme D168Q


proof_lines: pH 6.5, 30°C, strain 1a, mutant enzyme R123T/D168Q

proof_lines: pH 7.5, 22°C, mutant enzyme GT-3a ( R123T/ D168Q)


substrate_name: EDVV-Abu-CSMSY

proof_lines: inhibition of enzyme mutant R155K, pH and temperature not specified in the publication

substrate: EDVV-Abu-CSMSY


proof_lines: mutant V36A, in the presence of cofactor peptide KKGSVVIVGRIVLSCK


proof_lines: mutant V36M/R155K, in the presence of cofactor peptide KKGSVVIVGRIVLSCK


proof_lines: mutant V36L, in the presence of cofactor peptide KKGSVVIVGRIVLSCK


proof_lines: mutant V36M, in the presence of cofactor peptide KKGSVVIVGRIVLSCK


proof_lines: mutant enzyme M485A/V524A/Q526A, at 23°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:P27958; reconstructed_variant_sequence using NS3 residue offset +1026 and applied mutation signature V78A/D79E/Q80G with inferred residue-numbering offset +1026 to reconstruct the assayed variant sequence.

proof_lines: genotype 2 mutant V78A/D79E/Q80G, 30°C, pH 6.5


sequence_variant_note: Resolved canonical sequence from UniProtKB:P27958; reconstructed_variant_sequence using NS3 residue offset +1026 and applied mutation signature T160A with inferred residue-numbering offset +1026 to reconstruct the assayed variant sequence.


organism: Hepatitis C virus genotype 1b

sequence_source: UniProtKB:P26662; canonical reviewed accession sequence

proof_lines: pH 7.5, temperature not specified in the publication


sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines:
mutant A156V, pH 7.8, 30°C
or above, mutant A156V, pH 7.8, 30°C


proof_lines:
mutant A156T, pH 7.8, 30°C
or above, mutant A156T, pH 7.8, 30°C


proof_lines: genotype 2 and 3 mutant V132L, 30°C, pH 6.5


proof_lines: genotype 2 mutant S122K/V132L, 30°C, pH 6.5


proof_lines: genotype 3 mutant R123T/D168Q, 30°C, pH 6.5


proof_lines: genotype 2 mutant V78A/D79E/Q80G/S122K/V321L, 30°C, pH 6.5


proof_lines: genotype 3 mutant R123T/D168Q/V132L, 30°C, pH 6.5


proof_lines:
genotype 1b enzyme, 30°C, pH 6.5
genotype 3 enzyme, 30°C, pH 6.5
genotype 2 enzyme, 30°C, pH 6.5


verification_status: unverified

status: unverified


proof_lines:
NS3 mutant D129A
mutant D129A, pH 9.5, 37°C


proof_lines:
NS3 mutant D129E
mutant D129E, pH 9.5, 37°C


organism: Yellow fever virus

substrate_name: Boc-Gly-Arg-Arg-AMC

substrate: Boc-Gly-Arg-Arg-AMC


enzyme_display_name: oligopeptidase B

organism: Escherichia coli (strain K12)

substrate_name: benzoyl-Arg 4-nitroanilide

enzyme: oligopeptidase B

substrate: benzoyl-Arg 4-nitroanilide


organism: Serratia proteamaculans

source_db: oed

sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB B3VI58 canonical sequence checked 2026-06-19 plus selected-row mutation_signature and applied mutation signature K660A to reconstruct the assayed variant sequence.

data_origin: experimental

source: oed


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB B3VI58 canonical sequence checked 2026-06-19 plus selected-row mutation_signature and applied mutation signature D649A to reconstruct the assayed variant sequence.


sequence_source: reconstructed from UniProtKB B3VI58 canonical sequence checked 2026-06-19 plus selected-row mutation_signature


sequence_source: UniProtKB B3VI58 canonical sequence checked 2026-06-19


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB B3VI58 canonical sequence checked 2026-06-19 plus selected-row mutation_signature and applied mutation signature D647A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB B3VI58 canonical sequence checked 2026-06-19 plus selected-row mutation_signature and applied mutation signature E125A to reconstruct the assayed variant sequence.


kcat_over_km_origin: calculated


proof_lines: recombinant wild-type enzyme, pH 8.0, 25°C


proof_lines: pH 8.0, 25°C, recombinant mutant protein E125A with a modified hinge region (PSPmod)


organism: Escherichia coli

substrate_name: N-benzoyl-arginine amide

substrate: N-benzoyl-arginine amide


proof_lines:
pH 8.0, 25°C
presence of 50 mM Ca2+, pH 8.0, 25°C


organism: Trypanosoma brucei


proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant R689A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant E524Q, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant E172Q, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant E490Q, pH not specified in the publication, temperature not specified in the publication


organism: Trypanosoma cruzi


organism: Xenorhabdus nematophila

substrate_name: N-tosyl-Gly-Pro-Arg-paranitroanilide

substrate: N-tosyl-Gly-Pro-Arg-paranitroanilide


organism: Leishmania amazonensis

sequence_source: UniProtKB:A7XAB0 canonical sequence fetched by direct accession lookup on 2026-06-19


organism: Leishmania donovani

sequence_source: UniProtKB:C9EF60 canonical sequence fetched by direct accession lookup on 2026-06-19


organism: Leishmania major

sequence_source: UniProtKB:Q4QHU7 canonical sequence fetched by direct accession lookup on 2026-06-19

proof_lines: 21°C, pH not specified in the publication


organism: Salmonella enterica

sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0H3NCA6 canonical sequence plus residue-validated mutation reconstruction from selected BRENDA source note and applied mutation signature E624H to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0H3NCA6 canonical sequence plus residue-validated mutation reconstruction from selected BRENDA source note and applied mutation signature E494H/E624H/D599H to reconstruct the assayed variant sequence.

proof_lines: pH 8.0, 37°C, mutant E494H, E624H, and D599H


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0H3NCA6 canonical sequence plus residue-validated mutation reconstruction from selected BRENDA source note and applied mutation signature D599A/D638Q to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0H3NCA6 canonical sequence plus residue-validated mutation reconstruction from selected BRENDA source note and applied mutation signature D460T/D462N/E624H to reconstruct the assayed variant sequence.

proof_lines: pH 8.0, 37°C, opdB with polyhistidine affinity tag removed, mutant D460T, D462N and E624H


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0H3NCA6 canonical sequence plus residue-validated mutation reconstruction from selected BRENDA source note and applied mutation signature D567H to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0H3NCA6 canonical sequence plus residue-validated mutation reconstruction from selected BRENDA source note and applied mutation signature D462N to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0H3NCA6 canonical sequence plus residue-validated mutation reconstruction from selected BRENDA source note and applied mutation signature D460T/E494H to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0H3NCA6 canonical sequence plus residue-validated mutation reconstruction from selected BRENDA source note and applied mutation signature E494H/E624H to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0H3NCA6 canonical sequence plus residue-validated mutation reconstruction from selected BRENDA source note and applied mutation signature E578A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0H3NCA6 canonical sequence plus residue-validated mutation reconstruction from selected BRENDA source note and applied mutation signature E576A/E578A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0H3NCA6 canonical sequence plus residue-validated mutation reconstruction from selected BRENDA source note and applied mutation signature D599H to reconstruct the assayed variant sequence.


source_license: CC BY 4.0; CC BY-NC-ND 4.0

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: pH 8.0, 25°C, recombinant protein with a modified hinge region (PSPmod)

proof_lines: pH 8.0, 25°C, bacterial oligopeptidase B from Serratia proteomaculans (PSP)

proof_lines: substrate Nalpha-benzoyl-DL-Arg-4-nitroanilide, pH 8.0, 25°C

source_databases_merged: brenda skid


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: pH 8.0, 25°C, recombinant mutant protein E75 with a modified hinge region (PSPmod)


organism: Trypanosoma evansi

sequence_variant_note: Resolved canonical sequence from UniProt:Q6QDP1 plus PMID:15644339/GenBank AY546084; S563A reconstructed from canonical residue 563. and applied mutation signature S563A to reconstruct the assayed variant sequence.

proof_lines: pH 8.0, 37°C, ecombinant mutant enzyme S563A


sequence_source: UniProt:Q6QDP1 plus PMID:15644339/GenBank AY546084.

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines:
pH 8.0, 37°C, native enzyme
pH 8.0, 37°C, recombinant enzyme
pH 8.0, 37°C,recombinant enzyme


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0H3NCA6 direct REST sequence; reconstructed from selected mutation signature D460T and applied mutation signature D460T to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0H3NCA6 direct REST sequence; reconstructed from selected mutation signature E494H and applied mutation signature E494H to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0H3NCA6 direct REST sequence; reconstructed from selected mutation signature D460T/D462N and applied mutation signature D460T/D462N to reconstruct the assayed variant sequence.

proof_lines: pH 8.0, 37°C, mutant D460T and D460T/D462N


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0H3NCA6 direct REST sequence; reconstructed from selected mutation signature D638Q and applied mutation signature D638Q to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:A0A0H3NCA6 direct REST sequence

proof_lines: pH 8.0, 37°C, wild-type, mutant E494H, D599H and D567H

proof_lines: pH 8.0, 37°C, wild-type and mutant D599A


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0H3NCA6 direct REST sequence; reconstructed from selected mutation signature D567H/E624H and applied mutation signature D567H/E624H to reconstruct the assayed variant sequence.


proof_lines: pH 8.0, 37°C, opdB with polyhistidine affinty tag removed


sequence_variant_note: Resolved canonical sequence from UniProtKB:A0A0H3NCA6 direct REST sequence; reconstructed from selected mutation signature E576A and applied mutation signature E576A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B3VI58 direct REST sequence; reconstructed from selected mutation signature D647A and applied mutation signature D647A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B3VI58 direct REST sequence; reconstructed from selected mutation signature D649A and applied mutation signature D649A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B3VI58 direct REST sequence; reconstructed from selected mutation signature K655A and applied mutation signature K655A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B3VI58 direct REST sequence; reconstructed from selected mutation signature E125A and applied mutation signature E125A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B3VI58 direct REST sequence; reconstructed from selected mutation signature K660A and applied mutation signature K660A to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:B3VI58 direct REST sequence; reconstructed from selected mutation signature K655A


enzyme_display_name: ribulose-bisphosphate carboxylase

organism: Elymus farctus

proof_lines: 30°C, pH not specified in the publication

proof_lines: 25°C, pH not specified in the publication, presence of 21% O2

enzyme: ribulose-bisphosphate carboxylase


organism: Eragrostis tef

proof_lines: 25°C, pH not specified in the publication

proof_lines: 20°C, pH not specified in the publication


organism: Erythrina flabelliformis


organism: Euphorbia helioscopia


organism: Euphorbia microsphaera

proof_lines: 20°C, pH not specified in the publication, presence of 21% O2


organism: Festuca pratensis


organism: Flueggea suffruticosa


organism: Glycine canescens


organism: Glycine max


organism: Halothiobacillus neapolitanus

substrate_name: D-ribulose 1,5-bisphosphate

sequence_source: UniProtKB O85040 direct lookup

substrate: D-ribulose 1,5-bisphosphate


sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

proof_lines: apparent Km for mutant carboxysome lacking carbonic anhydrase


organism: Hordeum brachyantherum


organism: Hordeum murinum

proof_lines: 30°C, pH not specified in the publication, presence of 21% O2


organism: Lablab purpureus


organism: Lepidium campestre


organism: Lolium giganteum


organism: Synechococcus elongatus PCC 6301

sequence_source: UniProtKB REST direct lookup, release 2026_02, accession P00880; canonical sequence only, variant unresolved

proof_lines: mutant M259T/A575V/M57I, pH not specified in the publication, temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB REST direct lookup, release 2026_02, accession P00880 and applied mutation signature V186I/T327A to reconstruct the assayed variant sequence.

proof_lines: mutant V186I/T327A, pH not specified in the publication, temperature not specified in the publication


sequence_source: UniProtKB REST direct lookup, release 2026_02, accession P00880

proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB REST direct lookup, release 2026_02, accession P00880 and applied mutation signature T327A to reconstruct the assayed variant sequence.

proof_lines: mutant T327A, pH not specified in the publication, temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB REST direct lookup, release 2026_02, accession P00880 and applied mutation signature R214H/A375S to reconstruct the assayed variant sequence.

proof_lines: mutant R214H/A375S, pH not specified in the publication, temperature not specified in the publication


organism: Tephrosia candida

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


organism: Tephrosia purpurea


organism: Tephrosia rhodesica


organism: Thermococcus kodakarensis

sequence_variant_note: Resolved canonical sequence from UniProtKB REST direct lookup, release 2026_02, accession O93627 and applied mutation signature V330T to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme V330T, in 100 mM Bicine-NaOH (pH 8.3) and 10 mM MgCl2, temperature not specified in the publication


sequence_source: UniProtKB REST direct lookup, release 2026_02, accession O93627

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: wild type enzyme, in 100 mM Bicine-NaOH (pH 8.3) and 10 mM MgCl2, temperature not specified in the publication


organism: Thermosynechococcus vestitus

sequence_variant_note: Resolved canonical sequence from UniProtKB REST direct lookup, release 2026_02, accession Q8DIS5 and applied mutation signature I393M/P415A to reconstruct the assayed variant sequence.

proof_lines: mutation I393M/P415A in subunit RbcL, pH 8.1, 25°C


sequence_variant_note: Resolved canonical sequence from UniProtKB REST direct lookup, release 2026_02, accession Q8DIS5 and applied mutation signature F345I to reconstruct the assayed variant sequence.

proof_lines: mutation F345I in subunit RbcL, pH 8.1, 25°C


sequence_source: UniProtKB REST direct lookup, release 2026_02, accession Q8DIS5; canonical sequence only, variant unresolved

proof_lines: mutations A414T/P415A in subunit RbcL, plus mutation A48V in subunit RbcS, pH 8.1, 25°C


proof_lines: mutation P415A in subunit RbcL, plus mutations Y36N/G112D in subunit RbcS, pH 8.1, 25°C


sequence_variant_note: Resolved canonical sequence from UniProtKB REST direct lookup, release 2026_02, accession Q8DIS5 and applied mutation signature P415A to reconstruct the assayed variant sequence.

proof_lines: mutation P415A in subunit RbcL, pH 8.1, 25°C


proof_lines: mutation P415A in subunit RbcL, plus mutation H37L in subunit RbcS, pH 8.1, 25°C


organism: Oryza sativa Japonica Group

proof_lines: 30°C, pH not specified in the publication

proof_lines: 20°C, pH not specified in the publication

proof_lines: 25°C, pH not specified in the publication


organism: Oxybasis rubra

proof_lines: 25°C, pH not specified in the publication, presence of 21% O2


organism: Panicum amarum


organism: Panicum phragmitoides

proof_lines: 30°C, pH not specified in the publication, presence of 21% O2


organism: Paspalum dilatatum

proof_lines: Vmax carboxylation: 3.11 micromol/min/mg


organism: Phaseolus carteri

proof_lines: 20°C, pH not specified in the publication, presence of 21% O2


organism: Phaseolus coccineus


organism: Phaseolus lunatus


organism: Phaseolus vulgaris


organism: Poa palustris


organism: Puccinellia distans


organism: Puccinellia lemmonii


organism: Puccinellia maritima


organism: Puccinellia nuttalliana


organism: Pueraria montana


organism: Rhodospirillum rubrum

sequence_variant_note: Resolved canonical sequence from UniProtKB P04718 canonical sequence with H44Q substitution reconstructed during review and applied mutation signature H44Q to reconstruct the assayed variant sequence.

proof_lines:
mutant enzyme H44Q, at 25°C, pH 8
mutant enzyme H44Q, at 25 °C, pH 8


organism: Lolium multiflorum


organism: Lolium rigidum


organism: Macrotyloma uniflorum


organism: Manihot esculenta


organism: Mercurialis annua


organism: Musa velutina


organism: Nicotiana tabacum


organism: Oryza barthii x Oryza glaberrima


organism: Oryza eichingeri


organism: Oryza glaberrima


organism: Oryza glumipatula


organism: Oryza longistaminata


organism: Oryza meridionalis


organism: Oryza nivara


organism: Oryza punctata


organism: Oryza sativa Indica Group


organism: Blitum petiolare


organism: Bromus anomalus


organism: Calamagrostis arundinacea


organism: Calamagrostis canescens


organism: Calamagrostis foliosa


organism: Calamagrostis nutkaensis


organism: Calamagrostis stricta subsp. inexpansa


organism: Chenopodiastrum murale


organism: Chrysanthellum indicum


organism: Citrullus ecirrhosus


organism: Citrullus lanatus


organism: Cynodon dactylon

proof_lines: Vmax carboxylation: 3.41 micromol/min/mg


organism: Deschampsia danthonioides


organism: Desmodium cinereum


organism: Desmodium intortum


organism: Desmodium psilocarpum


organism: Unknown


organism: Abelmoschus esculentus


organism: Acidithiobacillus ferrooxidans


organism: Acidovorax facilis


organism: Acinetobacter sp.


organism: Acrosiphonia arcta


organism: Agave victoriae-reginae

assay_conditions_summary: pH 8; 298.15 K; buffer context but no accession

proof_lines: in 100 mM Bicine-NaOH pH 8.0, 20 mM MgCl2, at 25°C


organism: Alaria esculenta


organism: Allochromatium vinosum

assay_conditions_summary: pH 8


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines:
mutant rbcL-rbcS
mutants rbcL-rbcS, rbcA-rbcD


organism: Amaranthus hybridus


organism: Aphanothece halophytica


organism: Thermosynechococcus vestitus

sequence_source: UniProtKB Q8DIS5 direct lookup for canonical RbcL only; full multisubunit variant sequence not resolved.

proof_lines: mutation P415A in subunit RbcL, plus mutation V98M in subunit RbcS, pH 8.1, 25°C


sequence_variant_note: Resolved canonical sequence from UniProtKB Q8DIS5 direct lookup; RbcL variant reconstructed from checked 1-indexed residues. and applied mutation signature F345I/P415A to reconstruct the assayed variant sequence.

proof_lines: mutation F345I/P415A in subunit RbcL, pH 8.1, 25°C


sequence_variant_note: Resolved canonical sequence from UniProtKB Q8DIS5 direct lookup; RbcL variant reconstructed from checked 1-indexed residues. and applied mutation signature V300A to reconstruct the assayed variant sequence.

proof_lines: mutation V300A in subunit RbcL, pH 8.1, 25°C


sequence_variant_note: Resolved canonical sequence from UniProtKB Q8DIS5 direct lookup; RbcL variant reconstructed from checked 1-indexed residues. and applied mutation signature A414T/P415A to reconstruct the assayed variant sequence.

proof_lines: mutation A414T/P415A in subunit RbcL, pH 8.1, 25°C


sequence_variant_note: Resolved canonical sequence from UniProtKB Q8DIS5 direct lookup; RbcL variant reconstructed from checked 1-indexed residues. and applied mutation signature L74M/D397N/P415A to reconstruct the assayed variant sequence.

proof_lines: mutations L74M/D397N/P415A in subunit RbcL, pH 8.1, 25°C


proof_lines: mutation P415A in subunit RbcL, plus mutation Y36N/R51H in subunit RbcS, pH 8.1, 25°C


sequence_variant_note: Resolved canonical sequence from UniProtKB Q8DIS5 direct lookup; RbcL variant reconstructed from checked 1-indexed residues. and applied mutation signature A398T/P415A to reconstruct the assayed variant sequence.

proof_lines: mutation A398T/P415A in subunit RbcL, pH 8.1, 25°C


sequence_source: UniProtKB Q8DIS5 direct lookup.


sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

proof_lines: mutation P415A in subunit RbcL, plus mutation A48M in subunit RbcS, pH 8.1, 25°C


organism: Triticum aestivum

sequence_source: UniProtKB A0A1C3HPU2 direct lookup returned an inactive/deleted entry with no live sequence.

proof_lines: 25°C, pH not specified in the publication

proof_lines: 30°C, pH not specified in the publication


sequence_source: UniProtKB P11383 direct lookup.


organism: Triticum monococcum subsp. aegilopoides

sequence_source: UniProtKB A0A1C3HPT9 direct lookup returned an inactive/deleted entry with no live sequence.

proof_lines: 20°C, pH not specified in the publication


organism: Zoysia japonica

sequence_source: UniProtKB B0B758 direct lookup returned an inactive/deleted entry with no live sequence.

proof_lines: Vmax carboxylation: 3.78 micromol/min/mg


organism: Rhodospirillum rubrum

sequence_variant_note: Resolved canonical sequence from UniProtKB P04718 canonical sequence plus selected mutation_signature H44N and applied mutation signature H44N to reconstruct the assayed variant sequence.

proof_lines:
mutant enzyme H44N, at 25°C, pH 8
mutant enzyme H44N, at 25 °C, pH 8


sequence_source: UniProtKB P04718 canonical sequence from direct REST lookup

proof_lines: wild-type, cosubstrate: D-ribulose 1,5-bisphosphate


sequence_variant_note: Resolved canonical sequence from UniProtKB P04718 canonical sequence plus selected mutation_signature D117H and applied mutation signature D117H to reconstruct the assayed variant sequence.

proof_lines:
mutant enzyme D117H, at 25°C, pH 8
mutant enzyme D117H, at 25 °C, pH 8


sequence_variant_note: Resolved canonical sequence from UniProtKB P04718 canonical sequence plus selected mutation_signature D117V and applied mutation signature D117V to reconstruct the assayed variant sequence.

proof_lines:
mutant enzyme D117V, at 25°C, pH 8
mutant enzyme D117V, at 25 °C, pH 8


sequence_variant_note: Resolved canonical sequence from UniProtKB P04718 canonical sequence plus selected mutation_signature D263Q and applied mutation signature D263Q to reconstruct the assayed variant sequence.

proof_lines: mutant D263Q, cosubstrate: D-ribulose 1,5-bisphosphate


sequence_variant_note: Resolved canonical sequence from UniProtKB P04718 canonical sequence plus selected mutation_signature D263N and applied mutation signature D263N to reconstruct the assayed variant sequence.

proof_lines: mutant D263N, cosubstrate: D-ribulose 1,5-bisphosphate


sequence_variant_note: Resolved canonical sequence from UniProtKB P04718 canonical sequence plus selected mutation_signature D263E and applied mutation signature D263E to reconstruct the assayed variant sequence.

proof_lines: mutant D263E, cosubstrate: D-ribulose 1,5-bisphosphate


sequence_variant_note: Resolved canonical sequence from UniProtKB P04718 canonical sequence plus selected mutation_signature D263S and applied mutation signature D263S to reconstruct the assayed variant sequence.

proof_lines: mutant D263S, cosubstrate: D-ribulose 1,5-bisphosphate


sequence_variant_note: Resolved canonical sequence from UniProtKB P04718 canonical sequence plus selected mutation_signature D263A and applied mutation signature D263A to reconstruct the assayed variant sequence.

proof_lines: mutant D263A, cosubstrate: D-ribulose 1,5-bisphosphate


organism: Skeletonema costatum

sequence_source: UniProtKB A0A097IV41 canonical sequence from direct REST lookup

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: pH 7.5, temperature not specified in the publication


organism: Sphenostylis stenocarpa

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: 20°C, pH not specified in the publication, presence of 21% O2

proof_lines: 25°C, pH not specified in the publication, presence of 21% O2


organism: Synechococcus elongatus PCC 6301

sequence_variant_note: Resolved canonical sequence from UniProtKB P00880 canonical sequence plus selected mutation_signature S325L/T327A and applied mutation signature S325L/T327A to reconstruct the assayed variant sequence.

proof_lines: mutant S325L/T327A, pH not specified in the publication, temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB P00880 canonical sequence plus selected mutation_signature S325L and applied mutation signature S325L to reconstruct the assayed variant sequence.

proof_lines: mutant S325L, pH not specified in the publication, temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB P00880 canonical sequence plus selected mutation_signature M259T and applied mutation signature M259T to reconstruct the assayed variant sequence.

proof_lines: mutant M259T, pH not specified in the publication, temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB P00880 canonical sequence plus selected mutation_signature R214H and applied mutation signature R214H to reconstruct the assayed variant sequence.

proof_lines: mutant R214H, pH not specified in the publication, temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProtKB P00880 canonical sequence plus selected mutation_signature V186I and applied mutation signature V186I to reconstruct the assayed variant sequence.

proof_lines: mutant V186I, pH not specified in the publication, temperature not specified in the publication


organism: Arabidopsis thaliana

proof_lines: in 100 mM Bicine-NaOH pH 8.0, 20 mM MgCl2, at 25°C


organism: Archaeoglobus fulgidus


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


organism: Atrichum undulatum


organism: Bradyrhizobium japonicum

proof_lines: carboxylation oxygenation


organism: Carpobrotus edulis


organism: Ceratophyllum demersum


organism: Chlamydomonas reinhardtii


organism: Galdieria sulphuraria


organism: Glycine max

proof_lines: carboxylase catalytic efficiency, pH 8.0, 25°C


organism: Griffithsia monilis


organism: Helianthus annuus


organism: Hibiscus cannabinus


organism: Himantothallus grandifolius


organism: Hordeum vulgare


organism: Hydrogenophaga pseudoflava


organism: Iris douglasiana


organism: Lactuca sativa

proof_lines: oxygenase catalytic efficiency, pH 8.0, 25°C


organism: Laminaria digitata


organism: Laminaria solidungula


organism: Devaleraea ramentacea


organism: Drosera capensis

sequence_source: UniProtKB:P28405 reviewed Swiss-Prot REST EC+organism lookup 2026-06-19


organism: Drosera venusta


organism: Echeveria elegans


organism: Eucalyptus moorei


organism: Eucalyptus neglecta


organism: Flaveria angustifolia

sequence_source: UniProtKB:E9N7A6 unreviewed TrEMBL REST EC+organism lookup 2026-06-19


organism: Flaveria bidentis

sequence_source: UniProtKB:P19161 reviewed Swiss-Prot REST EC+organism lookup 2026-06-19; E9N7A4 duplicate sequence checked


organism: Flaveria cronquistii

sequence_source: UniProtKB:E9N7A9 unreviewed TrEMBL REST EC+organism lookup 2026-06-19


organism: Flaveria floridana

sequence_source: UniProtKB:E9N7B0 unreviewed TrEMBL REST EC+organism lookup 2026-06-19


organism: Flaveria pringlei

sequence_source: UniProtKB:P19162 reviewed Swiss-Prot REST EC+organism lookup 2026-06-19; E9N7A3 duplicate sequence checked


organism: Flaveria trinervia

sequence_source: UniProtKB:E9N7A7 unreviewed TrEMBL REST EC+organism lookup 2026-06-19


organism: Chlamydomonas reinhardtii


organism: Citrus sinensis


organism: Coffea arabica


organism: Crithmum maritimum


organism: Cupriavidus necator


organism: Cycas panzhihuaensis


organism: Cylindrotheca fusiformis


organism: Cylindrotheca sp.


organism: Dactylis glomerata


organism: Desmarestia aculeata


organism: Nitrosomonas sp.


organism: Nymphaea alba


organism: Olisthodiscus luteus


organism: Oryza sativa

proof_lines:
nonoxygenic conditions, pH 8.0, 25°C
carboxylation
presence of 21% oxygen, pH 8.0, 25°C


organism: Pallenis maritima


organism: Palmaria decipiens


organism: Palmaria palmata


organism: Panicum dichotomiflorum

proof_lines: 20°C, pH not specified in the publication

proof_lines: 30°C, pH not specified in the publication


organism: Paracoccus denitrificans


organism: Phaeodactylum tricornutum

proof_lines:
cosubstrate: CO2
at pH 8.3


organism: Phycodrys rubens


organism: Platycerium superbum


organism: Porphyridium purpureum


organism: Pteridium aquilinum


organism: Ptilota gunneri


organism: Rhodospirillum rubrum


assay_conditions_summary: pH 8; 298.15 K; buffer CO2- and O2-free 200 mM Bicine-NaOH

proof_lines: mutant enzyme K122R, in CO2- and O2-free 200 mM Bicine-NaOH buffer (pH 8.0) containing 20 mM MgCl2, at 25°C


proof_lines: wild type enzyme, in CO2- and O2-free 200 mM Bicine-NaOH buffer (pH 8.0) containing 20 mM MgCl2, at 25°C


organism: Saccharina latissima


organism: Sarracenia flava


organism: Sideritis cretica subsp. spicata


organism: Sorghum bicolor

proof_lines: wild type enzyme, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, at 28°C


proof_lines: recombinant enzyme expressed in Oryza sativa transgenic line SS10, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0,

proof_lines: recombinant enzyme expressed in Oryza sativa transgenic line SS16, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0,

proof_lines: recombinant enzyme expressed in Oryza sativa transgenic line SS5, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, a


organism: Limonium stenophyllum


organism: Limonium virgatum


organism: Mallomonas papillosa


organism: Marchantia polymorpha


organism: Medicago sativa


organism: Metasequoia glyptostroboides


organism: Methanococcoides burtonii

assay_conditions_summary: pH 6

proof_lines:
pH 6.0, for both dimer and pentamer
pH 8.0, for both dimer and pentamer
pentamer


organism: Nicotiana tabacum

proof_lines:
pH 7.8, 25°C, mutant Val-335, in vitro
pH 7.8, 25°C, mutant Val-335, in vivo


proof_lines: rubisco oligomer (S40L)16 composed of linked small and large subunits, measured using fractions 7 and 8 following Superdex 200 HR purification

proof_lines: rubisco oligomer (S40L)8 composed of linked small and large subunits, measured using fractions 7 and 8 following Superdex 200 HR purification


proof_lines: wild-type, using soluble leaf protein extract

proof_lines: wild-type, measured using fractions 12 and 13 following Superdex 200 HR purification


proof_lines: enzyme assembled with small subunit isoform RbcS-S1, pH 8.0, 25°C

proof_lines: enzyme assembled with small subunit isoform RbcS-S5, pH 8.0, 25°C

proof_lines: enzyme assembled with small subunit isoform RbcS-S2, pH 8.0, 25°C


organism: Nitrobacter winogradskyi


organism: Spinacia oleracea


organism: Sulfobacillus thermosulfidooxidans


organism: Symphytum sp.


organism: Synechococcus

proof_lines: wild-type, co-substrate: D-ribulose 1,5-bisphosphate


proof_lines: mutant D103V, co-substrate: D-ribulose 1,5-bisphosphate


proof_lines:
mutant D103V/A375V, co-substrate: CO2
mutant D103V/A375V


proof_lines: mutant A375V, co-substrate: D-ribulose 1,5-bisphosphate


proof_lines: mutant A375V/D103V, co-substrate: D-ribulose 1,5-bisphosphate


organism: Synechococcus sp.

proof_lines:
pH 8.0, 23°C, wild-type
wild-type enzyme


organism: Agriophyllum squarrosum

proof_lines: 20°C, pH not specified in the publication, presence of 21% O2

proof_lines: 25°C, pH not specified in the publication

proof_lines: 30°C, pH not specified in the publication


organism: Agrostis scabra

proof_lines: 20°C, pH not specified in the publication


organism: Agrostis stolonifera

proof_lines: 30°C, pH not specified in the publication, presence of 21% O2


organism: Amphicarpaea bracteata


organism: Anethum foeniculum


organism: Arabidopsis thaliana

proof_lines: 30°C growth temperature, assay at 25°C, pH not specified in the publication

proof_lines: 20°C growth temperature, assay at 25°C, pH not specified in the publication

proof_lines: 10°C growth temperature, assay at 25°C, pH not specified in the publication


organism: Archaeoglobus fulgidus

sequence_variant_note: Resolved canonical sequence from UniProtKB:O28635 canonical FASTA plus selected BRENDA mutation note M295D and applied mutation signature M295D to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:O28635 canonical FASTA plus selected BRENDA mutation note M295D/S363I and applied mutation signature M295D/S363I to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:O28635 canonical FASTA plus selected BRENDA mutation note M295D/S363V and applied mutation signature M295D/S363V to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:O28635 canonical FASTA plus selected BRENDA mutation note S363I and applied mutation signature S363I to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:O28635 canonical FASTA plus selected BRENDA mutation note S363V and applied mutation signature S363V to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:O28635 direct FASTA lookup; reviewed EC 4.1.1.39 entry RBL_ARCFU


organism: Arctagrostis latifolia


organism: Artemisia myriantha


organism: Artemisia vulgaris


organism: Beta vulgaris


organism: Synechocystis sp.

sequence_source: UniProtKB:P54205; direct EC 4.1.1.39 + organism lookup

proof_lines: wild type enzyme, in 50 mM HEPES, pH 8.0, at 30°C

proof_lines: pH 8.0, 30°C, wild-type, oxidized, mutant C172A, reduced


sequence_source: UniProtKB:P54205; canonical context only, not row-specific variant sequence

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P54205; canonical context only, not row-specific variant sequence and applied mutation signature R134A to reconstruct the assayed variant sequence.


proof_lines: mutant enzyme G404A, in 50 mM HEPES, pH 8.0, at 30°C


proof_lines: mutant enzyme T65S, in 50 mM HEPES, pH 8.0, at 30°C


proof_lines: mutant enzyme T65A, in 50 mM HEPES, pH 8.0, at 30°C


organism: Synura petersenii


organism: Synura uvella


organism: Teucrium heterophyllum

proof_lines: in 100 mM Bicine-NaOH pH 8.0, 20 mM MgCl2, at 25°C


organism: Thermococcus kodakarensis

sequence_source: UniProtKB:O93627; direct EC 4.1.1.39 + organism lookup


organism: Thermosynechococcus vestitus

sequence_source: UniProtKB:Q8DIS5; direct EC 4.1.1.39 + organism lookup


organism: Thiobacillus denitrificans

proof_lines:
enzyme form II
enzyme form I


organism: Synechococcus sp.

sequence_variant_note: Resolved canonical sequence from UniProtKB P00880 canonical FASTA plus PMID 15067012 mutation evidence and applied mutation signature G176D to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB P00880 canonical FASTA plus PMID 15067012 mutation evidence and applied mutation signature G176A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB P00880 canonical FASTA plus PMID 15067012 mutation evidence and applied mutation signature G176N to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB P54205 canonical FASTA plus PMID 12591867 mutation evidence and applied mutation signature C192A with inferred residue-numbering offset -5 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB P54205 canonical FASTA plus PMID 12591867 mutation evidence and applied mutation signature C172A with inferred residue-numbering offset +15 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB P54205 canonical FASTA plus PMID 12591867 mutation evidence and applied mutation signature C172A/C192A with inferred residue-numbering offset -5 to reconstruct the assayed variant sequence.

proof_lines: pH 8.0, 30°C, mutant C172A/C192A, reduced


sequence_source: UniProtKB P54205 canonical FASTA plus PMID 15937184 mutation evidence

sequence_variant_note: Mutation signature K305E matches the mutant residue at offset -5 and the reference residue at offset +6; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_variant_note: Mutation signature H310D could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


verification_status: corrected

sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P54205 and applied mutation signature K305A with inferred residue-numbering offset -5 to reconstruct the assayed variant sequence.

column_label: Table 2 (Kinetic properties of Rubisco mutants), strain row 'K305A'; column 'Site' = Latch; column 'Kcat (min-1)' = 177; column 'Km(RuBP) (uM)' = 199; column 'apparent Km (CO2) (uM)' = 185; column 'Ki(Pi) (mM)' = NM. Table 2 prints no kcat/Km column.

normalized_value_unit_evidence: kcat: printed Table 2 cell 177 under the column head Kcat (min-1); one min^-1 is 1/60 s^-1, so 177 / 60 = 2.95 s^(-1). km: printed Table 2 cell 199 under the column head Km(RuBP) (uM); one uM is 1/1000 mM, so 199 / 1000 = 0.199 mM. kcat/Km: Table 2 has no kcat/Km column, so nothing is converted for the ratio; the canonical catalytic efficiency is the quotient of those two converted cells of the same strain row, 2.95 / 0.199 = 14.824120603015075 s^(-1)*mM^(-1), typed derived_same_measurement against them.

raw_value_unit_evidence: Table 2, strain row K305A, site Latch: Kcat = 177 min^-1; Km(RuBP) = 199 uM. Table 2 prints no kcat/Km column, and this article prints no catalytic-efficiency cell for this measurement.

row_label: Table 2, strain row K305A, site Latch

table_label: Table 2: Kinetic properties of Rubisco mutants (Journal of Bacteriology 2005, 187(12):4222-4228)

status: corrected


sequence_variant_note: Mutation signature H327Q matches the mutant residue at offset -5 and the reference residue at offset -7; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


organism: Thiomicrospira thyasirae

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


organism: Trachycarpus fortunei


organism: Trichormus variabilis


organism: Triticum aestivum

proof_lines: enzyme from well-watered plant grown at 38°C, pH and temperature not specified in the publication

proof_lines: enzyme from well-watered plant grown at 25°C, pH and temperature not specified in the publication


organism: Zea mays

proof_lines:
at pH 8.3
nonoxygenic conditions, pH 8.0, 25°C
Km (CO2): 0.146 mbar, pH 7.95, 25°C


enzyme_display_name: aryldialkylphosphatase

organism: Homo sapiens

substrate_name: paraoxon

assay_conditions_summary: pH 7.4; 310.15 K; buffer 20 mM Tris-HCl

proof_lines: in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication

proof_lines: in 50 mM Tris-HCl buffer, 10 mM CaCl2, pH 7.4

enzyme: aryldialkylphosphatase

substrate: paraoxon


organism: Brevundimonas diminuta

substrate_name: diethyl-paraoxon

sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 plus mutation_signature reconstruction and applied mutation signature F132V/H254Q/H257F/S308L to reconstruct the assayed variant sequence.

proof_lines: pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L

substrate: diethyl-paraoxon


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 plus mutation_signature reconstruction and applied mutation signature I106G/F132V/H254Q/H257F to reconstruct the assayed variant sequence.

proof_lines: pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 plus mutation_signature reconstruction and applied mutation signature A80V/F132V/K185R/H254Q/H257Y/I274N to reconstruct the assayed variant sequence.

proof_lines: pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 plus mutation_signature reconstruction and applied mutation signature A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L to reconstruct the assayed variant sequence.

proof_lines: pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 plus mutation_signature reconstruction and applied mutation signature A80V/F132V/K185R/H254Q/H257Y/I274N/S308L to reconstruct the assayed variant sequence.

proof_lines: pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 plus mutation_signature reconstruction and applied mutation signature A80V/I106C/F132V/K185R/H254Q/H257Y/I274N to reconstruct the assayed variant sequence.

proof_lines: pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 plus mutation_signature reconstruction and applied mutation signature A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L to reconstruct the assayed variant sequence.

proof_lines: pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 plus mutation_signature reconstruction and applied mutation signature A80V/I106G/F132V/K185R/H254Q/H257Y/I274N to reconstruct the assayed variant sequence.

proof_lines: pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 plus mutation_signature reconstruction and applied mutation signature I106C/F132V/H254Q/H257F/S308L to reconstruct the assayed variant sequence.

proof_lines: pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 plus mutation_signature reconstruction and applied mutation signature I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L to reconstruct the assayed variant sequence.


substrate_name: malathion

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: mutant enzyme BHR-70, at pH 8.0 and 30Â°C

proof_lines: mutant enzyme BHR-72, at pH 8.0 and 30Â°C

proof_lines: mutant enzyme BHR-74, at pH 8.0 and 30Â°C


substrate_name: sarin

sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 plus mutation_signature reconstruction and applied mutation signature C59V/C227V/S267M to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme C59V/C227V/S267M, at pH 8.0 and 37Â°C

substrate: sarin


substrate_name: VR

sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 plus mutation_signature reconstruction and applied mutation signature C59V/C227V to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme C59V/C227V, at pH 8.0 and 37Â°C

substrate: VR


substrate_name: cyclosarin

sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 plus mutation_signature reconstruction and applied mutation signature C59V/C227V/S267L to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme C59V/C227V/S267L, at pH 8.0 and 37Â°C

substrate: cyclosarin


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 plus mutation_signature reconstruction and applied mutation signature C59L/C227A/S267M to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme C59L/C227A/S267M, at pH 8.0 and 37Â°C


sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.

proof_lines: mutant enzyme C59L/C227A/D203N, at pH 8.0 and 37Â°C


organism: Agrobacterium tumefaciens

substrate_name: methyl parathion

sequence_source: UniProtKB:Q93LD7 direct REST lookup on 2026-06-19

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: pH 9.1, 37°C, recombinant enzyme in absence of Co2+

proof_lines: pH 9.1, 37°C, recombinant enzyme in presence of Co2+

proof_lines: pH 8.0, 37°C, enzyme administered in monkeys in vivo

substrate: methyl parathion


substrate_name: diethyl 4-chlorophenyl thiophosphate

sequence_source: UniProtKB:P0A434 direct REST lookup on 2026-06-19

assay_conditions_summary: pH 9; 303.15 K; buffer tripart

proof_lines: recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C

proof_lines: pH 9.0, 30°C, recombinant wild-type enzyme

substrate: diethyl 4-chlorophenyl thiophosphate


substrate_name: demeton-S

sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 direct REST lookup on 2026-06-19 and applied mutation signature H254R/H257F to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 8; 298.15 K; buffer tripart

proof_lines: mutant enzyme H254R/H257F, in tripart buffer (pH 8.0)

proof_lines: mutant enzyme H254R/H257F, in 20 mM CHES buffer (pH 9.0) at 25°C

substrate: demeton-S


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 direct REST lookup on 2026-06-19 and applied mutation signature H254R/H257L to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme H254R/H257L, in tripart buffer (pH 8.0)

proof_lines: mutant enzyme H254R/H257L, in 20 mM CHES buffer (pH 9.0) at 25°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 direct REST lookup on 2026-06-19 and applied mutation signature H254R to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 9; 298.15 K; buffer 20 mM CHES

proof_lines: mutant enzyme H254R, in 20 mM CHES buffer (pH 9.0) at 25°C

proof_lines: mutant enzyme H254R, in tripart buffer (pH 8.0)


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 direct REST lookup on 2026-06-19 and applied mutation signature H257L to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme H257L, in tripart buffer (pH 8.0)

proof_lines: mutant enzyme H257L, in 20 mM CHES buffer (pH 9.0) at 25°C


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 direct REST lookup on 2026-06-19 and applied mutation signature H123I to reconstruct the assayed variant sequence.

proof_lines: Co2+-PTE mutant H123I, at pH 8.5 and 30°C, with paraoxon as substrate


proof_lines: Co2+-PTE, at pH 8.5 and 30°C, with paraoxon as substrate


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 direct REST lookup on 2026-06-19 and applied mutation signature H123N to reconstruct the assayed variant sequence.

proof_lines: Co2+-PTE mutant H123N, at pH 8.5 and 30°C, with paraoxon as substrate


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 direct REST lookup on 2026-06-19 and applied mutation signature H123A to reconstruct the assayed variant sequence.

proof_lines: Co2+-PTE mutant H123A, at pH 8.5 and 30°C, with paraoxon as substrate


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 direct REST lookup on 2026-06-19 and applied mutation signature I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 direct REST lookup on 2026-06-19 and applied mutation signature I106C/F132V/H254Q/H257F to reconstruct the assayed variant sequence.

proof_lines: pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 direct REST lookup on 2026-06-19 and applied mutation signature I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 direct REST lookup on 2026-06-19 and applied mutation signature F132V/H254Q/H257F to reconstruct the assayed variant sequence.

proof_lines: pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 direct REST lookup on 2026-06-19 and applied mutation signature H254Q/H257F to reconstruct the assayed variant sequence.

proof_lines: pH 9.0, 30°C, recombinant mutant H254Q/H257F


sequence_variant_note: Resolved canonical sequence from UniProtKB:P0A434 direct REST lookup on 2026-06-19 and applied mutation signature I106G/F132V/H254Q/H257F/S308L to reconstruct the assayed variant sequence.

proof_lines: pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L


sequence_source: UniProtKB reviewed P27169 canonical sequence checked as context only


sequence_source: UniProtKB reviewed P27169 canonical FASTA sequence

proof_lines: pH 8.0, 25°C, recombinant wild-type enzyme


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProtKB reviewed P27169 canonical sequence and selected source mutation signature H115W and applied mutation signature H115W to reconstruct the assayed variant sequence.

proof_lines:
mutant H115W, pH 8.0, 25°C
pH 8.0, 25°C, recombinant mutants H115W
pH 10.5, 25°C, mutant enzyme H115W


proof_lines: pH 8.0, 25°C, recombinant mutant H115W/R192K


substrate_name: dimethyl-paraoxon

proof_lines: pH 8.0, 25°C, recombinant mutant H115W/R192W

substrate: dimethyl-paraoxon


proof_lines: pH 8.0, 25°C, recombinant mutant H115W/R192S


proof_lines: pH 8.0, 25°C, recombinant mutant H115W/R192A


proof_lines: pH 8.0, 25°C, recombinant mutant H115W/R192I


proof_lines: pH 8.0, 25°C, recombinant mutant H115W/R192N


organism: Mus musculus

sequence_source: UniProtKB reviewed Q62087 canonical FASTA sequence

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


organism: Oryctolagus cuniculus

sequence_source: UniProtKB reviewed Q9BGN0 canonical FASTA sequence


substrate_name: Unknown

sequence_source: UniProtKB reviewed P27170 canonical FASTA sequence

proof_lines: pH 10.5, 25°C, recombinant wild-type enzyme

substrate: Unknown


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProtKB reviewed P27170 canonical sequence and selected source mutation signature I74F/H115W and applied mutation signature I74F/H115W to reconstruct the assayed variant sequence.

proof_lines: pH 10.5, 25°C, recombinant mutant I74F/H115W


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProtKB reviewed P27170 canonical sequence and selected source mutation signature V346A and applied mutation signature V346A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProtKB reviewed P27170 canonical sequence and selected source mutation signature I74F/T332S and applied mutation signature I74F/T332S to reconstruct the assayed variant sequence.

proof_lines: pH 10.5, 25°C, recombinant mutant I74F/T332S


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProtKB reviewed P27170 canonical sequence and selected source mutation signature T332S and applied mutation signature T332S to reconstruct the assayed variant sequence.


substrate_name: VX

sequence_variant_note: Resolved canonical sequence from UniProtKB P0A434 direct lookup with residue-checked reconstruction and applied mutation signature I106A/C59V/C227V/S267M to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme I106A/C59V/C227V/S267M, at pH 8.0 and 37Â°C

substrate: VX


organism: Escherichia coli K-12

substrate_name: chlorpyrifos

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

substrate: chlorpyrifos


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines:
pH 8.0, 37°C, genetic variant A1467
pH 8.0, 37°C, genetic variant A1030
pH 8.0, 37°C, genetic variant B1368


organism: Flavobacterium sp.

sequence_source: UniProtKB P0A433 direct lookup

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

assay_conditions_summary: pH 9; 310.15 K; buffer 50 mM CHES/CoCl2

proof_lines: surface-displayed recombinant enzyme from fresh cells, in 50 mM CHES/CoCl2 buffer, pH 9.0, with 0.05 mM CoCl2, at 37°C

proof_lines: surface-displayed recombinant enzyme from freeze-thawed cells, in 50 mM CHES/CoCl2 buffer, pH 9.0, with 0.05 mM CoCl2, at 37°C

proof_lines: intracellular-expressed recombinant enzyme from fresh cells, in 50 mM CHES/CoCl2 buffer, pH 9.0, with 0.05 mM CoCl2, at 37°C


proof_lines: pH 8.0, recombinant genetic variant G3C9

proof_lines: pH 8.0, recombinant genetic variant G2E6


proof_lines: pH 8.0, recombinant genetic variant G2C2

proof_lines: pH 8.0, recombinant genetic variant G3A5

proof_lines: pH 8.0, recombinant genetic variant G3G3


sequence_variant_note: Resolved canonical sequence from UniProtKB P27169 direct lookup with residue-checked reconstruction and applied mutation signature E313A to reconstruct the assayed variant sequence.


proof_lines: mutant E314A and mutant G11C, pH 10.5, 25°C


sequence_variant_note: Resolved canonical sequence from UniProtKB P27169 direct lookup with residue-checked reconstruction and applied mutation signature N133S to reconstruct the assayed variant sequence.


proof_lines: mutant G11A and mutant G11S, pH 10.5, 25°C


sequence_variant_note: Resolved canonical sequence from UniProtKB P27169 direct lookup with residue-checked reconstruction and applied mutation signature H115W/N133S to reconstruct the assayed variant sequence.

proof_lines: pH 10.5, 25°C, mutant enzyme H115W/N133S


sequence_source: UniProtKB P27169 direct lookup

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines:
pH 8.3, activity buffer
pH 8.0
recombinant enzyme


sequence_variant_note: Resolved canonical sequence from UniProtKB P27169 direct lookup with residue-checked reconstruction and applied mutation signature H134Q to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB P27169 direct lookup with residue-checked reconstruction and applied mutation signature H115Q to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB P27169 direct lookup with residue-checked reconstruction and applied mutation signature H115Q/H134Q to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB P27169 direct lookup with residue-checked reconstruction and applied mutation signature Q192R to reconstruct the assayed variant sequence.

proof_lines: mutant enzyme Q192R, in 20 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C

proof_lines: mutant enzyme Q192R, in 50 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C


organism: Saccharolobus solfataricus

sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB:Q97VT7 canonical sequence, accessed 2026-06-19 and applied mutation signature W263F to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:Q97VT7 canonical sequence, accessed 2026-06-19


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB:Q97VT7 canonical sequence, accessed 2026-06-19 and applied mutation signature C258L/I261F/W263A to reconstruct the assayed variant sequence.

proof_lines: recombinant enzyme mutant C258L/I261F/W263A, pH 8.5, 25°C, with 0.025% SDS

proof_lines: recombinant enzyme mutant C258L/I261F/W263A, pH 8.5, 65°C, with 0.025% SDS


organism: Sphingobium sp. TCM1

substrate_name: diethyl 3-fluorophenyl phosphate

sequence_source: UniProtKB:A0A077JBW9 canonical sequence, accessed 2026-06-19

proof_lines: pH and temperature not specified in the publication


organism: Sulfolobus acidocaldarius

substrate_name: parathion

sequence_source: UniProtKB:Q4J6Z8 canonical sequence, accessed 2026-06-19

substrate: parathion


organism: Vulcanisaeta moutnovskia

substrate_name: methyl paraoxon

sequence_source: UniProtKB:F0QXN6 canonical sequence, accessed 2026-06-19

substrate: methyl paraoxon


proof_lines: mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C


proof_lines: mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C


proof_lines: wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C


proof_lines: mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C


organism: Lucilia cuprina

substrate_name: diethylumbelliferyl phosphate

proof_lines: pH 7.5, 25°C, recombinant mutant enzyme G137D

substrate: diethylumbelliferyl phosphate


substrate_name: diethyl paraoxon

assay_conditions_summary: pH 7.4; buffer context but no accession

proof_lines: in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication

substrate: diethyl paraoxon


organism: Pimelobacter simplex

substrate_name: ethyl parathion

substrate: ethyl parathion


organism: Pseudomonas sp.

substrate_name: O,O-diethyl fluorophosphate

proof_lines: pH 8.0, 25°C, enzyme administered in mice in vivo

proof_lines: pH 8.0, 38°C, enzyme administered in mice in vivo

substrate: O,O-diethyl fluorophosphate


organism: Purpureocillium lilacinum

substrate_name: malathion

substrate: malathion


organism: Rattus norvegicus

substrate_name: paraoxon

proof_lines:
pH 8.5, 37°C, enzyme form M1
pH 8.5, 37°C, enzyme form M2
37°C, pH 8.5

substrate: paraoxon


proof_lines: with 50% (v/v) glycerol, at pH 8.0 and -18°C


organism: Sphingopyxis terrae subsp. terrae

substrate_name: triphenyl phosphate

substrate: triphenyl phosphate


sequence_variant_note: Resolved canonical sequence from UniProt REST FASTA P27170; reconstructed variant I74F and applied mutation signature I74F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST FASTA P27170; reconstructed variant I74F/H115W/T332S and applied mutation signature I74F/H115W/T332S to reconstruct the assayed variant sequence.

proof_lines: pH 10.5, 25°C, recombinant mutant I74F/H115W/T332S


sequence_variant_note: Resolved canonical sequence from UniProt REST FASTA P27170; reconstructed variant H115W/T332S and applied mutation signature H115W/T332S to reconstruct the assayed variant sequence.

proof_lines: pH 10.5, 25°C, recombinant mutant H115W/T332S


organism: Saccharolobus islandicus

sequence_source: UniProt REST FASTA C4KKZ9

proof_lines: 25°C, pH not specified in the publication, wild-type enzyme


proof_lines: 25°C, 0.01% SDS, pH not specified in the publication

proof_lines: 70°C, pH not specified in the publication


sequence_variant_note: Resolved canonical sequence from UniProt REST FASTA C4KKZ9; reconstructed variant E14K and applied mutation signature E14K to reconstruct the assayed variant sequence.

proof_lines: 25°C, pH not specified in the publication, mutant enzyme E14K


sequence_variant_note: Resolved canonical sequence from UniProt REST FASTA C4KKZ9; reconstructed variant Y34Q and applied mutation signature Y34Q to reconstruct the assayed variant sequence.

proof_lines: 25°C, pH not specified in the publication, mutant enzyme Y34Q


sequence_variant_note: Resolved canonical sequence from UniProt REST FASTA C4KKZ9; reconstructed variant E14K/Y34Q and applied mutation signature E14K/Y34Q to reconstruct the assayed variant sequence.

proof_lines: 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q


sequence_source: UniProt REST FASTA B5BLW5

assay_conditions_summary: pH 7; 333.15 K; buffer 100 mM sodium phosphate

proof_lines: recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C


sequence_variant_note: Resolved canonical sequence from UniProt REST FASTA Q97VT7; reconstructed variant Y97W/I261F and applied mutation signature Y97W/I261F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST FASTA Q97VT7; reconstructed variant Y97W/W263Q and applied mutation signature Y97W/W263Q to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST FASTA Q97VT7; reconstructed variant Y97W/W263F and applied mutation signature Y97W/W263F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST FASTA Q97VT7; reconstructed variant Y97W/I98F/I261F and applied mutation signature Y97W/I98F/I261F to reconstruct the assayed variant sequence.

proof_lines: pH 8.0, 70°C, mutant enzyme Y97W/I98F/I261F


sequence_source: UniProt REST FASTA Q97VT7

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


sequence_variant_note: Resolved canonical sequence from UniProt REST FASTA Q97VT7; reconstructed variant Y97W and applied mutation signature Y97W to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt REST FASTA Q97VT7; reconstructed variant W263Q and applied mutation signature W263Q to reconstruct the assayed variant sequence.


organism: Bos taurus

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


organism: Brevundimonas diminuta

assay_conditions_summary: pH 10.5; 298.15 K; buffer 50 mM borate

proof_lines: pH 10.5, 25°C, 3-fluorotyrosine-containing recombinant His6-tagged OPH


proof_lines: pH 10.5, 25°C, recombinant His6-tagged wild-type OPH


organism: Escherichia coli

substrate_name: methylparathion

proof_lines: pH 8.0, 37°C, recombinant His6-tagged wild-type OPH

substrate: methylparathion


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

assay_conditions_summary: pH 7.5; 298.15 K; buffer 50 mM HEPES

proof_lines: mutant enzyme A80V/I106V/F132D/K185R/D208G/H257W/I274N/R319S, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C


substrate_name: demeton-S methyl

proof_lines: mutant enzyme G60V/A80V/I106V/F132D/K185R/D208G/H257W/I274N/F306V/R319S, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C

substrate: demeton-S methyl


proof_lines: mutant enzyme G60V/A80V/I106V/F132D/K185R/D208G/H257W/I274N/R319S, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C


proof_lines: wild type enzyme, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C


proof_lines: mutant L271A/Y309A, pH 8.5, temperature not specified in the publication


assay_conditions_summary: pH 8.5; 298.15 K; buffer 50 mM HEPES

proof_lines: expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, pH 8.5, temperature not specified in the publication

proof_lines: mutant enzyme A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C


organism: Geobacillus stearothermophilus

proof_lines:
at 35°C, pH 9.0
at 35°C, pH 7.2


organism: Homo sapiens

substrate_name: sarin

proof_lines: mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C

substrate: sarin


organism: synthetic construct

substrate_name: methyl parathion

assay_conditions_summary: pH 9; 298.15 K; buffer 50 mM Tris

proof_lines: nanocapsule MIP-VBTNOH, at pH 9.0 and 30Â°C

proof_lines: nanocapsule MIP-VBTN, at pH 9.0 and 30Â°C

proof_lines: recombinant PON1, in 50 mM Tris buffer, 1 mM NaCl, pH 8.0 at 25°C

substrate: methyl parathion


assay_conditions_summary: pH 8; 298.15 K; buffer 50 mM Tris

proof_lines: wild-type, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C


proof_lines: mutant H115W, in 50 mM Tris buffer, 1 mM CaCl2, pH 8.0, 25°C


organism: Rhizopus microsporus var. oligosporus

substrate_name: myo-inositol hexakisphosphate

proof_lines:
pH 5.5
isozyme RO2, pH 4.5, 40°C
isozyme RO1, pH 4.5, 40°C

substrate: myo-inositol hexakisphosphate


organism: Unknown


organism: Aspergillus fumigatus

assay_conditions_summary: pH 5.5; 310.15 K; buffer 200 mM sodium acetate

proof_lines: in 200 mM sodium acetate buffer (pH 5.5), at 37°C


organism: Aspergillus japonicus

substrate_name: myo-inositol hexakisphosphate disodium salt

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.


organism: Aspergillus niger

substrate_name: p-nitrophenyl phosphate

proof_lines: in 1 M sodium acetate buffer pH 5.5, at 37°C

substrate: p-nitrophenyl phosphate


substrate: myo-inositol hexakisphosphate disodium salt


sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P34752 canonical sequence with reviewed mutation reconstruction from selected mutation_signature and applied mutation signature C215S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P34752 canonical sequence with reviewed mutation reconstruction from selected mutation_signature and applied mutation signature C31G to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P34752 canonical sequence with reviewed mutation reconstruction from selected mutation_signature and applied mutation signature C264G to reconstruct the assayed variant sequence.


proof_lines: pH 5.0, 58°C, recombinant wild-type enzyme


proof_lines: recombinant PP-NPep-6A mutant enzyme, pH 5.5, 37°C


proof_lines: recombinant mutant Q172R/K432R/Q368E/F376Y, pH 5.5, 37°C


proof_lines: recombinant mutant Q368E/K432R, pH 5.5, 37°C


proof_lines: recombinant mutant Q172R/K432R/Q368E/F376Y/T195L, pH 5.5, 37°C


proof_lines: recombinant mutant Q172R/K432R, pH 5.5, 37°C


proof_lines: recombinant mutant T195L/Q368E/F376Y, pH 5.5, 37°C


organism: Bacillus subtilis

sequence_source: UniProtKB:P42094 direct REST lookup on 2026-06-19

proof_lines: pH 7.0, 37°C, recombinant wild-type enzyme

proof_lines: pH 4.5, 37°C, recombinant wild-type enzyme


proof_lines: pH 7.0, 37°C, recombinant mutant D24G/K265N

proof_lines: pH 4.5, 37°C, recombinant mutant D24G/K265N


proof_lines:
pH 7.0, 37°C, recombinant mutant D24G
pH 4.5, 37°C, recombinant mutant D24G


organism: Bacillus subtilis subsp. subtilis

sequence_source: UniProtKB:O31097 direct REST lookup on 2026-06-19


organism: Burkholderia sp. a13(2014)


organism: Debaryomyces castellii

sequence_source: UniProtKB:A2TBB4 direct REST lookup on 2026-06-19

assay_conditions_summary: pH 4; 310.15 K; buffer 200 mM sodium acetate

proof_lines: native enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C

proof_lines: recombinant enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C


substrate_name: phytate

sequence_source: UniProtKB:Q6RK08 direct REST lookup on 2026-06-19

proof_lines:
pH 4.0, 55°C
pH 4.0, 55°C, 0.0005 mM orthovanadate
pH 4.0, 55°C, 0.010 mM metavanadate

substrate: phytate


organism: Hordeum vulgare


organism: Klebsiella sp.

sequence_source: UniProtKB:Q84CN9 direct REST lookup on 2026-06-19

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

proof_lines: pH not specified in the publication, temperature not specified in the publication


organism: Lactiplantibacillus plantarum

mutation_signature: truncated PHY200 protein

mutation_type: truncation


organism: Obesumbacterium proteus

sequence_source: UniProtKB:Q6U677 direct REST lookup on 2026-06-19


organism: Orbilia oligospora

sequence_source: UniProtKB:G1XN29 direct REST lookup on 2026-06-19


organism: Pantoea sp. 3.5.1

proof_lines: recombinant His-tagged enzyme, pH and temperature not specified in the publication


proof_lines: recombinant mutant Q172R/K432R/Q368E, pH 5.5, 37°C


proof_lines:
mutant N300K, pH 5, 40°C
mutant N300K, pH 2, 40°C
mutant N300K, pH 3, 40°C


proof_lines:
mutant D363N, pH 5, 40°C
mutant D363N, pH 6.5, 40°C
mutant D363N, pH 3, 40°C


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProtKB P34752 canonical sequence by applying A76M; checked 2026-06-19 and applied mutation signature A76M to reconstruct the assayed variant sequence.

proof_lines:
mutant A76M, pH 5, 40°C
mutant A76M, pH 6.5, 40°C
mutant A76M, pH 5.5, 40°C


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProtKB P34752 canonical sequence by applying S265P; checked 2026-06-19 and applied mutation signature S265P to reconstruct the assayed variant sequence.

proof_lines:
mutant S265P, pH 5, 40°C
mutant S265P, pH 2, 40°C
mutant S265P, pH 3, 40°C


organism: Bacillus amyloliquefaciens


organism: Bacillus sp. (in: firmicutes)


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProtKB O66037 canonical sequence by applying D308A; checked 2026-06-19 and applied mutation signature D308A to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 7

proof_lines: mutant D308A, pH 7.0, temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProtKB O66037 canonical sequence by applying D56E; checked 2026-06-19 and applied mutation signature D56E to reconstruct the assayed variant sequence.

proof_lines: mutant D56E, pH 7.0, temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProtKB O66037 canonical sequence by applying D56A; checked 2026-06-19 and applied mutation signature D56A to reconstruct the assayed variant sequence.

proof_lines: mutant D56A, pH 7.0, temperature not specified in the publication


sequence_source: UniProtKB O66037 canonical sequence REST direct check 2026-06-19

proof_lines: wild-type, pH 7.0, temperature not specified in the publication

proof_lines: wild-type, pH 6.0, temperature not specified in the publication

proof_lines: wild-type, pH 5.0, temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProtKB O66037 canonical sequence by applying D56L; checked 2026-06-19 and applied mutation signature D56L to reconstruct the assayed variant sequence.

proof_lines: mutant D56L, pH 7.0, temperature not specified in the publication


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProtKB O66037 canonical sequence by applying D308E; checked 2026-06-19 and applied mutation signature D308E to reconstruct the assayed variant sequence.

proof_lines: mutant D308E, pH 7.0, temperature not specified in the publication


proof_lines: pH 7.0, 37°C, recombinant mutant D24G/K70R/K111E/N121S

proof_lines: pH 4.5, 37°C, recombinant mutant D24G/K70R/K111E/N121S


organism: Arabidopsis thaliana

substrate_name: phosphoenolpyruvate

substrate: phosphoenolpyruvate


organism: Aspergillus carbonarius


organism: Aspergillus ficuum

substrate_name: 4-nitrophenyl phosphate

proof_lines:
recombinant enzyme
native fungal enzyme
pH 5.0, 58°C

substrate: 4-nitrophenyl phosphate


organism: Aspergillus flavipes


organism: Aspergillus fumigatus


organism: Aspergillus neoniger