organism: Escherichia coli (strain K12)

substrate_name: pppGpp

proof_lines: kcat is 0.023 sec(-1) for pppGpp hydrolase activity. kcat is 1.1 sec(-1) for the exopolyphosphatase activity.

substrate: pppGpp


enzyme_display_name: UDP-glucose 4,6-dehydratase

organism: Trichomonas vaginalis

substrate_name: dTDP-D-glucose

source_db: brenda

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

protein_accession_database: UniProt

source_license: CC BY 4.0

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

enzyme: UDP-glucose 4,6-dehydratase

substrate: dTDP-D-glucose

source: brenda


organism: Acanthamoeba polyphaga mimivirus

substrate_name: UDP-alpha-D-glucose

source_db: uniprot

kcat_display: Not reported

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: kcat is 0.27 sec(-1) for UDP-alpha-D-glucose (PubMed:32083779). kcat is 0.18 sec(-1) for dTDP-alpha-D-glucose (PubMed:32083779).

substrate: UDP-alpha-D-glucose

source: uniprot


organism: Arabidopsis thaliana

substrate_name: UDP-glucose

proof_lines: kcat is 2860 sec(-1) with UDP-glucose as substrate.

substrate: UDP-glucose


substrate_name: UDP-D-glucose

proof_lines: recombinant RHM2-N protein (amino acids 1–370)

substrate: UDP-D-glucose


organism: Chlorovirus heliozoae


organism: Silene dioica


enzyme_display_name: melilotate 3-monooxygenase

organism: Arthrobacter sp.

substrate_name: melilotate

assay_conditions_summary: cofactor NADH

enzyme: melilotate 3-monooxygenase

substrate: melilotate


organism: Unknown


organism: Bacillus anthracis

substrate_name: UDP-N-acetyl-D-glucosamine

km_unit: mM

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

mutation_type: substitution

substrate: UDP-N-acetyl-D-glucosamine


organism: Pseudomonas sp.


mutation_signature: stopped flow stopped flow

mutation_type: truncation


enzyme_display_name: 4-methylthio 2-oxobutanoate reductase (NADH)

organism: Homo sapiens

substrate_name: oxaloacetate

km_display: Not reported

substrate: oxaloacetate


enzyme_display_name: arginine--pyruvate transaminase

organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

substrate_name: pyruvate

enzyme: arginine--pyruvate transaminase

substrate: pyruvate


organism: Pseudomonas aeruginosa

assay_conditions_summary: pH 9; 310.15 K; cofactor NAD+

proof_lines: calculated, pH 9.0, 37°C, coupled reaction with NAD+ and L-alanine dehydrogenase


organism: Thermus thermophilus

substrate_name: tRNAPhe containing uridine at position 54

proof_lines: pH not specified in the publication, 60°C


enzyme_display_name: HECT-type E3 ubiquitin transferase

enzyme: HECT-type E3 ubiquitin transferase


substrate_name: adenosine 5'-phosphosulfate

proof_lines: cosubstrate adenosine 5-phosphorofluoridate, pH 10.6, 30°C

substrate: adenosine 5'-phosphosulfate


organism: Auxenochlorella pyrenoidosa

substrate_name: ammonia

substrate: ammonia


organism: Chlorella sp.


substrate_name: BCKDHA

proof_lines: kcat is 17.2 min(-1) for BCKDHA. kcat is 43.2 sec(-1) for p-nitrophenylphosphate.

substrate: BCKDHA


organism: Bos taurus

substrate_name: Unknown

ki_unit: mM

substrate: Unknown


substrate_name: 4-nitrophenyl phosphate

proof_lines: mutant N94K, presence of Mg2+, 37°C, pH 7.5

proof_lines: mutant N94K, presence of Mn2+, 30°C, pH 7.5

substrate: 4-nitrophenyl phosphate


proof_lines: wild-type, presence of Mg2+, 37°C, pH 7.5

proof_lines: wild-type, presence of Mn2+, 30°C, pH 7.5


enzyme_display_name: thiosulfate--dithiol sulfurtransferase

organism: Scenedesmus fuscus

substrate_name: dithioerythritol

proof_lines: pH 9, 37°C, with dithioerythritol as second substrate

proof_lines: pH 9, 37°C, with thiosulfate as second substrate, isoenzyme TSR II

enzyme: thiosulfate--dithiol sulfurtransferase

substrate: dithioerythritol


organism: Phormidium uncinatum

substrate_name: thiosulfate

proof_lines: with dithioerythritol as second substrate

substrate: thiosulfate


organism: thermophilic iron-oxidizing bacterium strain TI-1

assay_conditions_summary: pH 6; 318.15 K; cofactor NADPH


organism: Phanerodontia chrysosporium

substrate_name: dithiothreitol

substrate: dithiothreitol


enzyme_display_name: C-terminal processing peptidase

organism: Escherichia coli

enzyme: C-terminal processing peptidase


proof_lines:
wild-type enzyme, mutants V229Q, V229E
wild-type enzyme


substrate_name: Ac-WARAAARAAARBAAB

substrate: Ac-WARAAARAAARBAAB


organism: Spinacia oleracea

substrate_name: C-terminal 19 amino acids of preD1 protein


enzyme_display_name: calmodulin-lysine N-methyltransferase

organism: Ovis aries

substrate_name: VU-1 calmodulin

enzyme: calmodulin-lysine N-methyltransferase

substrate: VU-1 calmodulin


enzyme_display_name: rhamnogalacturonan galacturonohydrolase

organism: Aspergillus aculeatus

enzyme: rhamnogalacturonan galacturonohydrolase


enzyme_display_name: indoxyl-UDPG glucosyltransferase

organism: Persicaria tinctoria

substrate_name: UDP-glucose

enzyme: indoxyl-UDPG glucosyltransferase


organism: Strobilanthes cusia


enzyme_display_name: 1,4-beta-D-xylan synthase

organism: Acer pseudoplatanus

substrate_name: UDP-D-xylose

enzyme: 1,4-beta-D-xylan synthase

substrate: UDP-D-xylose


organism: Arabidopsis thaliana

proof_lines: microsomal fractions of stems from wild-type plants


organism: Hordeum vulgare

substrate_name: pyridylaminated beta-(1,4)-xylotriose

substrate: pyridylaminated beta-(1,4)-xylotriose


enzyme_display_name: medium-chain-enoyl-CoA hydratase

organism: Unknown

assay_conditions_summary: cofactor CoA

enzyme: medium-chain-enoyl-CoA hydratase


organism: Populus trichocarpa

proof_lines: co-substrate: xylotetraose, Km determined in microsomes from poplar stems. pH and temperature not specified in the publication

proof_lines: co-substrate: UDP-D-xylose, Km determined in microsomes from poplar stems. pH and temperature not specified in the publication

proof_lines: co-substrate: xylotetraose, Km determined in microsomes co-expressing PtrGT43B and PtrGT43C. pH and temperature not specified in the publication


organism: Rattus norvegicus

proof_lines: irreversible inhibition, covalent modification shown by peptide mass spectrometry, catalytic residue Glu47 covalently labelled by the inhibitor


organism: Sus scrofa

assay_conditions_summary: 298.15 K; cofactor CoA


enzyme_display_name: ATP-polyphosphate phosphotransferase

organism: Corynebacterium glutamicum

substrate_name: Hexametaphosphate

enzyme: ATP-polyphosphate phosphotransferase

substrate: Hexametaphosphate


organism: Cytophaga hutchinsonii

substrate_name: AMP

source_db: oed

substrate: AMP

source: oed


substrate_name: ATP

assay_conditions_summary: pH 8; 303.15 K; cofactor ATP

substrate: ATP


substrate_name: dADP

substrate: dADP


substrate_name: ADP

assay_conditions_summary: pH 8; 303.15 K; cofactor ADP

substrate: ADP


substrate_name: GDP

substrate: GDP


substrate_name: GMP

substrate: GMP


proof_lines:
pH 8.0, 30°C
pH 8.0, 30°C, cosubstrate: ADP


substrate_name: dAMP

substrate: dAMP


organism: Deinococcus radiodurans


source_license: CC BY 4.0; CC BY-NC-ND 4.0

proof_lines:
pH 8.0, 30°C
pH 8.0, 30°C, cosubstrate: ADP
Raw SKiD Env: Temp=30°C, pH=8

source_databases_merged: brenda skid


organism: Delftia tsuruhatensis

assay_conditions_summary: pH 7; 310.15 K; cofactor ADP


organism: Escherichia coli

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP


substrate_name: ADP; Polyphosphate

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

substrate: ADP; Polyphosphate


organism: Francisella tularensis subsp. tularensis

assay_conditions_summary: pH 8; 310.15 K; cofactor ADP


assay_conditions_summary: pH 8; 310.15 K; cofactor ATP

proof_lines:
pH 8.0, 37°C
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=37°C, pH=8


substrate_name: GTP

substrate: GTP


assay_conditions_summary: cofactor ATP

proof_lines: mutant enzyme K66A, pH and temperature not specified in the publication

proof_lines: mutant K66A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant enzyme D192A, pH and temperature not specified in the publication

proof_lines: mutant D192A, pH not specified in the publication, temperature not specified in the publication


proof_lines: wild-type enzyme, pH and temperature not specified in the publication

proof_lines: wild-type, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant D62A, pH not specified in the publication, temperature not specified in the publication

proof_lines: mutant enzyme D62A, pH and temperature not specified in the publication


proof_lines: mutant enzyme R178A, pH and temperature not specified in the publication

proof_lines: mutant R178A, pH not specified in the publication, temperature not specified in the publication


proof_lines: mutant D117N, pH not specified in the publication, temperature not specified in the publication

proof_lines: mutant enzyme D117N, pH and temperature not specified in the publication


proof_lines: mutant enzyme R118A, pH and temperature not specified in the publication

proof_lines: mutant R118A, pH not specified in the publication, temperature not specified in the publication


organism: Meiothermus ruber

assay_conditions_summary: cofactor ADP

proof_lines: mutant enzyme N121D, pH and temperature not specified in the publication


proof_lines: mutant N121D, pH not specified in the publication, temperature not specified in the publication


source_db: skid

source_license: CC BY-NC-ND 4.0

source: skid


organism: Mycobacterium tuberculosis

assay_conditions_summary: pH 8.5; 298.15 K; cofactor ATP

proof_lines: polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C

proof_lines: polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C


organism: Myxococcus xanthus


organism: Paenarthrobacter aurescens


proof_lines:
pH 8.0, 30°C
pH 8.0, 30°C, cosubstrate: ADP
Raw SKiD Env: Temp=30°C, pH=8


organism: Sinorhizobium meliloti


proof_lines: pH 8.0, 30°C, mutant enzyme H102K/A106E/V115T


organism: Pseudomonas aeruginosa

assay_conditions_summary: pH 7.2; 310.15 K; cofactor ATP


organism: Rhodocyclus sp.

assay_conditions_summary: pH 7.2; cofactor ATP

proof_lines: pH 7.2, room temperature, recombinant polyphosphate kinase


organism: Ruegeria pomeroyi


proof_lines: pH 8.0, 30°C, recombinant isozyme PPK2-2


substrate_name: UDP

substrate: UDP


proof_lines: pH 8.0, 30°C, recombinant isozyme PPK2-1


proof_lines: pH 8.0, 30°C, recombinant isozyme PPK2-3


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

proof_lines: kcat is 57.02 sec(-1) for hydrolase activity with polyP(75) as substrate. kcat is 53.03 sec(-1) for hydrolase activity with polyP(65) as substrate. kcat is 41.23 sec(-1) for hydrolase activity with polyP(45) as substrate. kcat is 40.20 sec(


assay_conditions_summary: pH 7; 310.15 K; cofactor ATP

proof_lines: isoform PPK2, pH and temperature not specified in the publication

proof_lines: isoform PPK1, pH and temperature not specified in the publication

proof_lines: in 50 mM Tris-HCl pH-7.5, 10 mM NH4SO4,10 mM KPO4, pH 7.0, 4 mM MgCl2, 50 mM NaCl, at 37°C


proof_lines: mutant H480A of isoform PPK1, pH and temperature not specified in the publication


proof_lines: mutant H510Q of isoform PPK1, pH and temperature not specified in the publication


proof_lines: mutant H480Q of isoform PPK1, pH and temperature not specified in the publication


substrate_name: (Phosphate)n

substrate: (Phosphate)n


assay_conditions_summary: pH 7.2; 303.15 K; cofactor ATP

proof_lines: pH 7.2, 30°C, recombinant wild-type enzyme


proof_lines: mutant Y524A of isoform PPK1, pH and temperature not specified in the publication


proof_lines: mutant H510A of isoform PPK1, pH and temperature not specified in the publication


mutation_signature: truncated enzyme


organism: Salmonella enterica subsp. enterica serovar Minnesota

assay_conditions_summary: pH 8; 298.15 K; cofactor ATP


organism: Sinomonas atrocyanea

assay_conditions_summary: pH 7; 303.15 K; cofactor ATP

proof_lines:
pH 7.0, 30°C
ATP in form of MnATP2-


organism: Sulfolobus acidocaldarius

assay_conditions_summary: pH 7; 348.15 K; cofactor ATP

proof_lines:
pH 7.0, 75°C
pH 7.0, 75°C, glycogen-complexed enzyme


organism: Burkholderia cepacia

assay_conditions_summary: pH 7; 293.15 K; cofactor ATP

proof_lines: pH 7.0, 20°C, polyphosphate kinase activity in cell extracts, detrmined with Robinson and Wood method

proof_lines: pH 7.0, 20°C, polyphosphate kinase activity in cell extracts, kinetic analysis


proof_lines: pH 7.5, 37°C, guanosine 5-tetraphosphate synthesis


organism: Mycolicibacterium smegmatis

substrate_name: Polyphosphate

assay_conditions_summary: pH 6; 310.15 K; cofactor ATP

substrate: Polyphosphate


organism: Neisseria meningitidis

substrate_name: phosphate

substrate: phosphate


enzyme_display_name: cyclomaltodextrinase

organism: Anoxybacillus flavithermus subsp. flavithermus

substrate_name: beta-cyclodextrin

enzyme: cyclomaltodextrinase

substrate: beta-cyclodextrin


substrate_name: gamma-cyclodextrin

substrate: gamma-cyclodextrin


organism: Evansella clarkii

substrate_name: maltooctaose

substrate: maltooctaose


organism: Flavobacterium sp. 92

substrate_name: alpha-cyclodextrin

substrate: alpha-cyclodextrin


organism: Geobacillus thermopakistaniensis


organism: Massilia timonae


organism: Pyrococcus furiosus

substrate_name: maltoheptaose

substrate: maltoheptaose


enzyme_display_name: serine racemase

organism: Homo sapiens

substrate_name: L-serine

enzyme: serine racemase

substrate: L-serine


proof_lines: pH 8.0, 37°C, recombinant wild-type enzyme


proof_lines: pH and temperature not specified in the publication


organism: Magallana gigas

proof_lines: pH 8.0, 30°C, recombinant mutant P150S/P151S/Y152S


proof_lines: pH 8.0, 30°C, recombinant wild-type enzyme


organism: Mus musculus

proof_lines: pH 8.0, 37°C, presence of 1 mM ATP, racemization reaction

proof_lines: pH 8.0, 37°C, presence of 1 mM ATP, elimination reaction


organism: Cicer arietinum

assay_conditions_summary: pH 7.5; 310.15 K; buffer HEPES


substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


organism: Drosophila melanogaster

substrate_name: KASA(iso-D)LAKY

substrate: KASA(iso-D)LAKY


organism: Sus scrofa


organism: Anoxybacillus flavithermus

substrate_name: soluble starch

substrate: soluble starch


organism: Caenorhabditis elegans


organism: Crassostrea gigas


substrate_name: D-serine

substrate: D-serine


organism: Dictyostelium discoideum


substrate_name: L-Serine

substrate: L-Serine


proof_lines: pH 8.0, 30°C, recombinant mutant P151S/N152S


organism: Penaeus monodon

proof_lines: pH 8.0, 30°C, recombinant mutant H150S/P151S/F152S


organism: Bacillus sp

substrate_name: alpha-cyclodextrin (at pH 7.0 and 50 degrees Celsius)

sequence_source: selected-batch source_record canonical-sequence context for UniProt Q59226

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

proof_lines: kcat is 78.18 sec(-1) with beta-cyclodextrin as substrate. kcat is 22.11 sec(-1) with amylose as substrate. kcat is 50.36 sec(-1) with amylopectin as substrate (PubMed:16536613). kcat is 101 min(-1) for dimeric form of the enzyme with beta-


sequence_source: selected-batch source_record canonical-sequence context for UniProt A0A7U9P668

proof_lines: kcat is 2739 sec(-1) with alpha-cyclodextrin as substrate. kcat is 1678 sec(-1) with beta-cyclodextrin as substrate. kcat is 821 sec(-1) with gamma-cyclodextrin as substrate. kcat value calculations are based on the dimeric enzyme.


organism: Rattus norvegicus

proof_lines:
cytosolic isozyme
recombinant protein
brush-border membrane isozyme


organism: Unknown


organism: Thermotoga neapolitana

substrate_name: p-nitrophenyl-D-maltopentaoside

proof_lines:
isoenzyme ag1B*
isoenzyme ag1B(1Met)
isoenzyme ag1B(2Met)

substrate: p-nitrophenyl-D-maltopentaoside


substrate_name: Unknown

substrate: Unknown


verification_method: compact batch and family-context review with direct UniProt accession lookup


organism: Pyrococcus horikoshii


organism: Roseobacter litoralis


proof_lines: pH 8.0, 30°C, recombinant His-tagged enzyme


proof_lines: pH 8.0, 30°C, recombinant mutant H150S/N152S


proof_lines: pH 8.0, 30°C, recombinant mutant H150S/P151S/N152S


proof_lines: pH 8.0, 30°C, recombinant mutant H150S/P151S


organism: Acropora millepora


proof_lines: pH 8.0, 30°C, recombinant mutant P150S/P151S/F152S


proof_lines: pH 8.0, 30°C, recombinant mutant P151S/F152S


organism: Escherichia coli


organism: Salmonella enterica


organism: Clostridioides difficile

proof_lines: pH 7.5, 37°C, recombinant wild-type enzyme


organism: Mus musculus

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ATP

proof_lines: mutant Q155D, racemization, pH 7.4, 37°C

proof_lines: mutant Q155D, presence of ATP, pH 7.4, 37°C

proof_lines: mutant Q155D, racemization, presence of ATP, pH 7.4, 37°C


proof_lines: wild-type, racemization, presence of ATP, pH 7.4, 37°C


organism: Oryza sativa

substrate_name: VYP-(L-iso-Asp)-HA

proof_lines:
isoform PIMT1, at pH 8.0 and 50°C
isoform PIMT1, at pH 8.0 and 45°C

substrate: VYP-(L-iso-Asp)-HA


organism: Thermoactinomyces vulgaris

substrate_name: starch

substrate: starch


proof_lines: mutant enzyme E219A/D225A, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication

proof_lines: mutant enzyme E219A/D225A, in the absence of Mg2+, pH and temperature not specified in the publication


proof_lines: wild type enzyme, in the presence of 1 mM Mg2+, pH and temperature not specified in the publication

proof_lines: wild type enzyme, in the absence of Mg2+, pH and temperature not specified in the publication


organism: Arabidopsis thaliana

substrate_name: VYP-L-isoAsp-HA

proof_lines:
native enzyme
at pH 7.5 and 25°C

substrate: VYP-L-isoAsp-HA


organism: Bos taurus

substrate_name: isoACTH

substrate: isoACTH


organism: Cicer arietinum

substrate_name: Val-Tyr-Pro(L-iso-aspartate)-His-Ala

substrate: Val-Tyr-Pro(L-iso-aspartate)-His-Ala


organism: Cucumis sativus

substrate_name: VYP-L-isoaspartyl-HA

assay_conditions_summary: pH 6; 318.15 K; buffer sodium citrate

proof_lines: in sodium citrate buffer at pH 6.0 and 45°C

substrate: VYP-L-isoaspartyl-HA


organism: Cyberlindnera jadinii

substrate_name: delta sleep inducing peptide L-isoaspartate


organism: Daucus carota


substrate_name: VYP (isoD) HA

substrate: VYP (isoD) HA


organism: Triticum aestivum


organism: Zea mays

proof_lines:
enzyme from seeds
enzyme from leaves


organism: Homo sapiens

substrate_name: ovalbumin

proof_lines: in 0.1 M sodium phosphate (pH 6.8), 1 mM EGTA, 0.004% (w/v) sodium azide, and 0.16% (v/v) Triton X-100, at 37°C

proof_lines: pH not specified in the publication, at 37°C

substrate: ovalbumin


organism: Pyrococcus furiosus


organism: Rhodopseudomonas palustris

substrate_name: VYR-isoD-RR

proof_lines:
isoform RPA0376, at pH 6.4 and 30°C
isoform RPA2838, at pH 6.4 and 30°C

substrate: VYR-isoD-RR


organism: Thermotoga maritima

substrate_name: KASA (isoD) LAKY

substrate: KASA (isoD) LAKY


enzyme_display_name: dolichyl-phosphatase

substrate_name: dolichyl phosphate

enzyme: dolichyl-phosphatase

substrate: dolichyl phosphate


organism: Glycine max


organism: Rattus norvegicus


enzyme_display_name: spheroidene monooxygenase

organism: Rhodobacter capsulatus

substrate_name: spirilloxanthin

proof_lines: pH 7.8, 28°C, formation of 2-ketospririlloxanthin

proof_lines: pH 7.8, 28°C, formation of 2,2-diketospririlloxanthin

enzyme: spheroidene monooxygenase

substrate: spirilloxanthin


organism: Rubrivivax gelatinosus

proof_lines: pH 7.8, 28°C, formation of 2,2-dioxospririlloxanthin

proof_lines: pH 7.8, 28°C, formation of 2-oxospririlloxanthin


enzyme_display_name: formate dehydrogenase (coenzyme F420)

organism: Methanobacterium formicicum

substrate_name: formate

assay_conditions_summary: pH 7.5; cofactor FAD

proof_lines: pH 7.5, temperature not specified in the publication, substrate: protioformate

proof_lines: pH 7.5, temperature not specified in the publication, substrate: deuterioformate

enzyme: formate dehydrogenase (coenzyme F420)

substrate: formate


organism: Methanobrevibacter ruminantium

assay_conditions_summary: pH 8

proof_lines: pH 8.0, temperature not specified in the publication


organism: Sus scrofa

substrate_name: C95-dolichyl phosphate

proof_lines: enzyme from grey matter of prenatal subjects

substrate: C95-dolichyl phosphate


organism: Archaeoglobus fulgidus

substrate_name: alpha-cyclodextrin

substrate: alpha-cyclodextrin


organism: Bacillus sp. (in: firmicutes)

substrate_name: amylose

assay_conditions_summary: pH 6

proof_lines:
pH 7.0, dodecamer
pH 7.0
pH 6.0

substrate: amylose


organism: Bacillus subtilis

substrate_name: maltoheptaose

substrate: maltoheptaose


organism: Flavobacterium sp.

substrate_name: beta-cyclodextrin

substrate: beta-cyclodextrin


organism: Geobacillus stearothermophilus

substrate_name: gamma-cyclodextrin

substrate: gamma-cyclodextrin


organism: Heyndrickxia coagulans

substrate_name: maltohexaose

substrate: maltohexaose


organism: Klebsiella oxytoca


organism: Laceyella sacchari


organism: Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)

substrate_name: spheroidene

substrate: spheroidene


substrate_name: coenzyme F420

assay_conditions_summary: cofactor FAD

substrate: coenzyme F420


organism: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

substrate_name: Unknown

substrate: Unknown


organism: Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534)

substrate_name: ATP

assay_conditions_summary: cofactor ATP

substrate: ATP


organism: Dictyostelium discoideum


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)


organism: Trypanosoma brucei brucei (strain 927/4 GUTat10.1)


organism: Trypanosoma cruzi (strain CL Brener)


organism: Arabidopsis thaliana

substrate_name: adenosine 5'-phosphoramidate (at pH 6.8)


organism: Homo sapiens

substrate_name: P(1)-P(3)-bis(5'-adenosyl) triphosphate

substrate: P(1)-P(3)-bis(5'-adenosyl) triphosphate


organism: Lysinibacillus sphaericus


organism: Niallia circulans


organism: Paenibacillus macerans


organism: Paenibacillus sp.


organism: Amphimedon queenslandica

substrate_name: D-serine

substrate: D-serine


organism: Anadara broughtonii

substrate_name: L-serine

substrate: L-serine


organism: Anneissia japonica


organism: Aplysia californica


enzyme_display_name: isoflavone 4'-O-methyltransferase

organism: Cicer arietinum

substrate_name: S-adenosyl-L-methionine

proof_lines: pH 8.8, 30°C, versus 7,4-dihydroxyisoflavone

proof_lines: pH 8.8, 30°C, versus S-adenosyl-L-methionine

enzyme: isoflavone 4'-O-methyltransferase

substrate: S-adenosyl-L-methionine


organism: Palaeococcus pacificus


organism: Pyrococcus furiosus

substrate_name: starch

proof_lines:
pH 4.5, 90°C, recombinant enzyme
pH 4.5, 90°C

substrate: starch


organism: unidentified


organism: Solanum lycopersicum


organism: Streptomyces garyphalus


organism: Bombyx mori


assay_conditions_summary: pH 8; 310.15 K; cofactor ATP

proof_lines:
in presence of ATP, pH 8.0, 37°C
pH 8.0
in absence of ATP, pH 8.0, 37°C


organism: Macrostomum lignano


organism: Manihot esculenta


organism: Mus musculus

proof_lines: racemization reaction, isoform A, pH 8.6, 37°C


organism: Pyrobaculum islandicum


organism: Rattus norvegicus


organism: Schizosaccharomyces pombe

proof_lines: pH and temperature not specified in the publication


organism: Escherichia coli

substrate_name: ATPgammaS

substrate: ATPgammaS


organism: Gallus gallus


assay_conditions_summary: pH 7.7; cofactor ATP


enzyme_display_name: limonene dehydrogenase

organism: Castellaniella defragrans

substrate_name: limonene

enzyme: limonene dehydrogenase

substrate: limonene


organism: Xanthobacter autotrophicus


organism: Glycine max


enzyme_display_name: [G-protein-coupled receptor] kinase

organism: Homo sapiens

substrate_name: rhodopsin

proof_lines: recombinant wild type enzyme, at pH 8.0 and 25°C

enzyme: [G-protein-coupled receptor] kinase

substrate: rhodopsin


proof_lines: recombinant mutant enzyme S500A, at pH 8.0 and 25°C


proof_lines: recombinant mutant enzyme T10A, at pH 8.0 and 25°C


organism: Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CCUG 56034 / CIP 105152 / NBRC 16084 / F199)

proof_lines: kcat is 25 min(-1) with 8'-apo-beta-carotenal as substrate. kcat is 8.4 min(-1) with 4'-apo-beta-carotenal as substrate. kcat is 0.2 min(-1) with gamma-carotene as substrate.


organism: Castellaniella defragrans (strain DSM 12143 / CCUG 39792 / 65Phen)

substrate_name: limonene (at 28 degrees Celsius)

substrate: limonene (at 28 degrees Celsius)


organism: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

substrate_name: maltotriose (at pH 6.5 and 85 degrees Celsius)


organism: Thermotoga neapolitana

substrate_name: beta-cyclodextrin (at 80 degrees Celsius)


enzyme_display_name: UDP-glucose 4,6-dehydratase

substrate_name: UDP-alpha-D-glucose

enzyme: UDP-glucose 4,6-dehydratase

substrate: UDP-alpha-D-glucose


organism: Aspergillus phoenicis


organism: Clostridium perfringens


substrate_name: 4-nitrophenyl alpha-D-mannopyranoside

proof_lines: in 100 mM sodium acetate at pH 4.0 and 37°C

proof_lines: in 100 mM sodium acetate (pH 4.0), at 37°C

substrate: 4-nitrophenyl alpha-D-mannopyranoside


enzyme_display_name: plasmalogen synthase

assay_conditions_summary: cofactor CoA

proof_lines:
submandibular gland
sublingual gland

enzyme: plasmalogen synthase


enzyme_display_name: 13-hydroxylupanine O-tigloyltransferase

organism: Lupinus albus

proof_lines:
with 13-alpha-hydroxylupanine
with 13-alpha-hydroxymultiflorine

enzyme: 13-hydroxylupanine O-tigloyltransferase


substrate_name: palmitoyl-CoA (in the presence of 1-palmitoyl-LPC as cosubstrate)


organism: Mus musculus


substrate_name: acetyl-CoA

substrate: acetyl-CoA


substrate_name: 13alpha-hydroxymultiflorine (with tigloyl-CoA as cosubstrate)


proof_lines: kcat is 5.4 min(-1) with alpha-D-Man-(1->3)-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol as substrate. kcat is 63.2 min(-1) with alpha-D-Man-(1->6)-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodo


organism: Oryctolagus cuniculus

substrate_name: tetrasaccharide-diphosphoryl-lipid

substrate: tetrasaccharide-diphosphoryl-lipid


substrate_name: GDP-alpha-D-mannose

substrate: GDP-alpha-D-mannose


substrate_name: N-acetyl-alpha-D-glucosaminyl-diphosphodolichol (at pH 7.5 and 37 degrees Celsius)

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.


organism: Cricetulus griseus


substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


organism: Vigna radiata


enzyme_display_name: exo-chitinase (non-reducing end)

organism: Pyrococcus furiosus

enzyme: exo-chitinase (non-reducing end)


organism: Sulfolobus acidocaldarius

substrate_name: UDP-N-acetyl-alpha-D-glucosamine

substrate: UDP-N-acetyl-alpha-D-glucosamine


organism: Bacillus licheniformis

substrate_name: colloidal chitin

substrate: colloidal chitin


enzyme_display_name: ADP deaminase

organism: Aspergillus melleus

substrate_name: adenosine

enzyme: ADP deaminase

substrate: adenosine


organism: Aspergillus ochraceus

substrate_name: Adenosine diphosphate

substrate: Adenosine diphosphate


enzyme_display_name: propanoyl-CoA:succinate CoA transferase

organism: Escherichia coli

substrate_name: propanoyl-CoA

assay_conditions_summary: pH 7; 298.15 K; cofactor CoA

enzyme: propanoyl-CoA:succinate CoA transferase

substrate: propanoyl-CoA


organism: Propionibacterium freudenreichii subsp. shermanii

substrate_name: acetate

assay_conditions_summary: pH 8; 298.15 K; cofactor CoA

proof_lines: with acetate as cosubstrate, at pH 8.0 and 25°C

substrate: acetate


organism: Escherichia coli (strain K12)

proof_lines: kcat is 0.72 sec(-1) with propanoyl-CoA as substrate.


organism: Salisediminibacterium selenitireducens

substrate_name: beta-D-glucose 1-phosphate

proof_lines:
pH 7.5, 30°C
pH 8.0, 30°C
Raw SKiD Env: Temp=30°C, pH=7.5

substrate: beta-D-glucose 1-phosphate


substrate_name: D-sorbitol 6-phosphate

substrate: D-sorbitol 6-phosphate


organism: Malus domestica


organism: Bacillus selenitireducens (strain ATCC 700615 / DSM 15326 / MLS10)

proof_lines: kcat is 95 sec(-1) with 2-O-alpha-D-glucopyranosyl-sn-glycerol.


substrate_name: fructose-1-P (with magnesium ions as cofactor and at pH 9)

proof_lines: kcat is 3.7 sec(-1) with fructose-1-P as substrate. kcat is 2.7 sec(-1) with ribose-5-P as substrate. kcat is 13 sec(-1) with glucose-6-P as substrate. kcat is 3.1 sec(-1) with fructose-6-P as substrate. kcat is 7.5 sec(-1) with beta-D-gluc


substrate_name: 2-deoxyglucose-6-P (with manganese ions as cofactor and at pH 9)

proof_lines: kcat is 33 sec(-1) with 2-deoxyglucose-6-P as substrate. kcat is 11 sec(-1) with mannose-6-P as substrate. kcat is 9.4 sec(-1) with 2-deoxyribose-5-P as substrate. kcat is 2.8 sec(-1) with ribose-5-P as substrate. kcat is 25 sec(-1) with gl


enzyme_display_name: oxamate carbamoyltransferase

substrate_name: oxamate

proof_lines: kcat is 58.5 sec(-1) with oxamate as substrate. kcat is 35.5 sec(-1) with carbamoyl phosphate as substrate.

enzyme: oxamate carbamoyltransferase

substrate: oxamate


substrate_name: Carbamoyl phosphate

proof_lines: pH 9.0, 30°C, recombinant His-tagged enzyme

substrate: Carbamoyl phosphate


organism: Bacillus subtilis (strain 168)

substrate_name: L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala

proof_lines: kcat is 5.7 sec(-1) with L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala as substrate. kcat is 2.6 sec(-1) with L-Ala-gamma-D-Glu-L-Lys-D-Ala as substrate. kcat is 1.2 sec(-1) with L-Ala-gamma-D-Glu-L-Lys as substrate.

substrate: L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala


organism: Bacillus subtilis

proof_lines: pH and temperature not specified in the publication


enzyme_display_name: indoleacetate decarboxylase

organism: Olsenella uli

proof_lines: mutant enzyme H514A, pH and temperature not specified in the publication

enzyme: indoleacetate decarboxylase


proof_lines: wild type enzyme, pH and temperature not specified in the publication


enzyme_display_name: mannose-6-phosphate 6-reductase

organism: Apium graveolens

substrate_name: D-mannitol 1-phosphate

assay_conditions_summary: cofactor NADPH

proof_lines:
enzyme purified from leaves
recombinant enzyme

enzyme: mannose-6-phosphate 6-reductase

substrate: D-mannitol 1-phosphate


organism: Phelipanche ramosa

substrate_name: D-mannose 6-phosphate

substrate: D-mannose 6-phosphate


organism: Striga hermonthica

substrate_name: NADPH

substrate: NADPH


enzyme_display_name: chrysanthemyl diphosphate synthase

organism: Artemisia spiciformis

substrate_name: dimethylallyl diphosphate

proof_lines: pH not specified in the publication, temperature not specified in the publication

enzyme: chrysanthemyl diphosphate synthase

substrate: dimethylallyl diphosphate


organism: Tanacetum cinerariifolium

proof_lines: pH 7.5, 30°C, His-tagged or detagged recombinant enzyme


organism: Clostridium scatologenes


organism: Tractidigestivibacter scatoligenes


proof_lines: kcat is 0.5 min(-1) with dimethylallyl diphosphate as substrate (PubMed:11287653). kcat is 0.0033 min(-1) with chrysanthemyl diphosphate as substrate (PubMed:25378387).


enzyme_display_name: 6beta-hydroxyhyoscyamine epoxidase

organism: Atropa belladonna

enzyme: 6beta-hydroxyhyoscyamine epoxidase


organism: Brugmansia sanguinea

proof_lines:
SUMO-tagged enzyme, at pH 7.6 and 34°C
untagged enzyme, at pH 7.6 and 34°C


organism: Datura metel


enzyme_display_name: Deleted entry

organism: Rattus norvegicus

substrate_name: H+; NADPH; Aquacob(III)alamin

enzyme: Deleted entry

substrate: H+; NADPH; Aquacob(III)alamin


enzyme_display_name: hydrogen-dependent carbon dioxide reductase

organism: Acetobacterium woodii


organism: Thermoanaerobacter kivui

substrate_name: formate

proof_lines:
at pH 7.0 and 30°C
at pH 7.0 and 60°C

enzyme: hydrogen-dependent carbon dioxide reductase

substrate: formate


mutation_type: deletion

proof_lines: variant FdhF_HycB3DELTA159-184 bioelectrode, at pH 6.2 and 25°C


enzyme_display_name: sulfide-cytochrome-c reductase (flavocytochrome c)

organism: Paracoccus pantotrophus

substrate_name: oxidized heart cytochrome c

substrate: oxidized heart cytochrome c


organism: Thiobacillus sp.

substrate_name: Sulfide

enzyme: sulfide-cytochrome-c reductase (flavocytochrome c)

substrate: Sulfide


organism: Anisodus tanguticus


organism: Hyoscyamus niger


organism: Thioalkalivibrio paradoxus

substrate_name: ferricytochrome c

substrate: ferricytochrome c


organism: Arabidopsis thaliana


organism: Gentiana triflora

substrate_name: caffeoyl-CoA

proof_lines: with delphinidin 3,5-diglucoside as cosubstrate

substrate: caffeoyl-CoA


enzyme_display_name: maltokinase

organism: Actinoplanes missouriensis

substrate_name: maltose (at pH 8)

enzyme: maltokinase

substrate: maltose (at pH 8)


organism: Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)

substrate_name: ATP (at 37 degrees Celsius and at pH 8)

substrate: ATP (at 37 degrees Celsius and at pH 8)


enzyme_display_name: Transferred entry: 2.4.3.8

organism: Escherichia coli

enzyme: Transferred entry: 2.4.3.8


organism: Neisseria meningitidis


substrate_name: ATP

assay_conditions_summary: pH 8.5; 295.15 K; cofactor ATP

substrate: ATP


organism: Mycobacterium tuberculosis variant bovis

proof_lines: pH 8.0, 37°C, recombinant His-tagged Mak


organism: Homo sapiens

substrate_name: CMP-N-acetylneuraminate; Fetuin

substrate: CMP-N-acetylneuraminate; Fetuin


substrate_name: CMP-N-acetylneuraminate; Colominate

substrate: CMP-N-acetylneuraminate; Colominate


enzyme_display_name: thymidylate 5'-phosphatase

substrate_name: AMP

enzyme: thymidylate 5'-phosphatase

substrate: AMP


organism: Bacillus subtilis

substrate_name: dTMP

substrate: dTMP


organism: Mus musculus

proof_lines: pH 7.5, 37°C, exponentially growing cells


enzyme_display_name: micrococcal nuclease

organism: Staphylococcus sp.

substrate_name: DNA

enzyme: micrococcal nuclease

substrate: DNA


enzyme_display_name: zinc D-Ala-D-Ala carboxypeptidase

organism: Enterococcus faecalis

substrate_name: D-Ala-D-Ala

proof_lines: determined by isothermal titration calorimetry, at pH 8.0 and 37°C

proof_lines: determined by ninhydrin/Cd(II) assay, at pH 8.0 and 37°C

enzyme: zinc D-Ala-D-Ala carboxypeptidase

substrate: D-Ala-D-Ala


organism: Streptomyces albus G

substrate_name: Unknown

substrate: Unknown


enzyme_display_name: methylphosphonate synthase

organism: Nitrosopumilus maritimus

proof_lines: temperature not specified in the publication, pH not specified in the publication

enzyme: methylphosphonate synthase


enzyme_display_name: phosphoribosyl-ATP diphosphatase

organism: Salmonella enterica subsp. enterica serovar Typhimurium

enzyme: phosphoribosyl-ATP diphosphatase


enzyme_display_name: guanosine-diphosphatase

substrate_name: IDP

enzyme: guanosine-diphosphatase

substrate: IDP


organism: Saccharomyces cerevisiae

substrate_name: GDP

substrate: GDP


enzyme_display_name: phosphoamidase

organism: Oryctolagus cuniculus

enzyme: phosphoamidase


organism: Rattus norvegicus

substrate_name: Phosphoramidate

substrate: Phosphoramidate


enzyme_display_name: CDP-glucose 4,6-dehydratase

substrate_name: CDP-D-glucose

enzyme: CDP-glucose 4,6-dehydratase

substrate: CDP-D-glucose


organism: Yersinia pseudotuberculosis


proof_lines:
wild-type enzyme
wild-type


substrate_name: CMP-acetylneuraminate

substrate: CMP-acetylneuraminate


enzyme_display_name: chlorogenate hydrolase

organism: Aspergillus niger

substrate_name: chlorogenic acid

enzyme: chlorogenate hydrolase

substrate: chlorogenic acid


organism: Bacillus amyloliquefaciens

substrate_name: chlorogenate

substrate: chlorogenate


organism: bacterium


organism: Ustilago maydis

substrate_name: methyl ferulate

substrate: methyl ferulate


substrate_name: methyl caffeate

substrate: methyl caffeate


organism: Acinetobacter baumannii

assay_conditions_summary: pH 7; cofactor ATP

proof_lines: pH 7.0, temperature not specified in the publication

proof_lines: pH 8.0, temperature not specified in the publication

proof_lines: pH 7.5, temperature not specified in the publication


organism: Candida albicans


organism: Homo sapiens


enzyme_display_name: aspulvinone dimethylallyltransferase

organism: Unknown

enzyme: aspulvinone dimethylallyltransferase


organism: Aspergillus terreus

substrate_name: dimethylallyl diphosphate

proof_lines: inhibition against dimethylallyl diphosphate

substrate: dimethylallyl diphosphate


enzyme_display_name: isatin hydrolase

organism: Burkholderia sp. IDO3

substrate_name: isatin

proof_lines: pH and temperature not specified in the publication

enzyme: isatin hydrolase

substrate: isatin


organism: Mesorhizobium loti


organism: Roseibium aggregatum (strain ATCC 25650 / DSM 13394 / JCM 20685 / NBRC 16684 / NCIMB 2208 / IAM 12614 / B1)

proof_lines: kcat is 24 sec(-1) for isatin as substrate.


organism: Ralstonia solanacearum


organism: Roseibium aggregatum


organism: Klebsiella aerogenes

substrate_name: mannitol

proof_lines: carbamoyl phosphate, D-mannose 6-phosphate, pH 7.5, 25°C

substrate: mannitol


enzyme_display_name: terephthalate 1,2-dioxygenase

organism: Comamonas sp.

substrate_name: NADPH

assay_conditions_summary: 303.15 K; cofactor NADPH

enzyme: terephthalate 1,2-dioxygenase

substrate: NADPH


enzyme_display_name: membrane Pro-Xaa carboxypeptidase

organism: Penicillium janthinellum

substrate_name: myoglobin

proof_lines: Michaelis-Menten kinetics in the bulk solution

enzyme: membrane Pro-Xaa carboxypeptidase

substrate: myoglobin


organism: Sus scrofa

substrate_name: Benzyloxycarbonyl-Pro-Met

substrate: Benzyloxycarbonyl-Pro-Met


enzyme_display_name: steroid 22S-hydroxylase

organism: Arabidopsis thaliana

proof_lines: kcat is 1.8 min(-1) for campesterol (at pH 7.25 and 30 degrees Celsius) (PubMed:16460510). kcat is 0.049 min(-1) for campestanol (at pH 7.25 and 30 degrees Celsius) (PubMed:16460510).

enzyme: steroid 22S-hydroxylase


organism: Locusta migratoria


enzyme_display_name: flavone synthase II

organism: Citrus japonica

substrate_name: isosakuranetin

enzyme: flavone synthase II

substrate: isosakuranetin


organism: Ustilago maydis


substrate_name: methyl p-coumarate

substrate: methyl p-coumarate


organism: Berberis koetineana

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


organism: Papaver somniferum

proof_lines: with (R,S)-6-O-methylnorlaudanosoline as substrate

proof_lines: with (R,S)-norlaudanosoline as substrate


enzyme_display_name: phenol O-methyltransferase

organism: Phanerodontia chrysosporium

enzyme: phenol O-methyltransferase


organism: Mycosarcoma maydis


organism: Homo sapiens

substrate_name: Acetaminophen; S-Adenosyl-L-methionine

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

substrate: Acetaminophen; S-Adenosyl-L-methionine


organism: Glandularia x hybrida

substrate_name: malonyl-CoA

assay_conditions_summary: pH 7; 303.15 K; cofactor CoA

substrate: malonyl-CoA


organism: Lamium purpureum


organism: Petroselinum crispum

substrate_name: kaempferol 3-O-glucoside

substrate: kaempferol 3-O-glucoside


substrate_name: 2-Naphthol glucoside

proof_lines: kcat is 40 sec(-1) for 2-Naphthol glucoside. kcat is 46 sec(-1) for 1-Naphthol glucoside. kcat is 33 sec(-1) for kaempferol 7-O-glucoside. kcat is 120 sec(-1) for 4-nitrophenyl glucoside. kcat is 19 sec(-1) for 4-methylumbelliferone glucosi

substrate: 2-Naphthol glucoside


organism: Nicotiana tabacum

substrate_name: kaempferol 7-O-glucoside (at 30 degrees Celsius)

proof_lines: kcat is 11.0 sec(-1) for kaempferol 7-O-glucoside. kcat is 6.8 sec(-1) for kaempferol 3-O-glucoside. kcat is 6.5 sec(-1) for 2-naphthol glucoside. kcat is 8.0 sec(-1) for malonyl-CoA. kcat is 9.3 sec(-1) for succinyl-CoA.


organism: Rhizoctonia solani

substrate_name: chlorogenate


organism: Oryza sativa

substrate_name: eriodictyol

substrate: eriodictyol


organism: Cavia porcellus


organism: Burkholderia pseudomallei


organism: Aspergillus niger

substrate_name: chlorogenic acid

substrate: chlorogenic acid


organism: Bacillus amyloliquefaciens

substrate: chlorogenate


substrate_name: WcbL; ATP

substrate: WcbL; ATP


organism: Geobacter metallireducens

substrate_name: acetyl-CoA

assay_conditions_summary: pH 6.8; 303.15 K; cofactor CoA

proof_lines: Km above 0.5 mM, with cyclohexane-1-carboxylate as cosubstrate, at pH 6.8 and 30°C

proof_lines: Km above 0.2 mM, with cyclohexane-1-carboxylate as cosubstrate, at pH 6.8 and 30°C

substrate: acetyl-CoA


enzyme_display_name: acetyl-CoA C-myristoyltransferase

organism: Rattus norvegicus

enzyme: acetyl-CoA C-myristoyltransferase


enzyme_display_name: diaminopimelate epimerase

organism: Unknown

substrate_name: meso-diaminoheptanedioate

enzyme: diaminopimelate epimerase

substrate: meso-diaminoheptanedioate


organism: Escherichia coli

substrate_name: LL-diaminoheptanedioate

substrate: LL-diaminoheptanedioate


organism: Mycobacterium tuberculosis

proof_lines: recombinant DapF consisting of silent mutation of the first 10 codons of the open reading frame


organism: Priestia megaterium


organism: Haemophilus influenzae

proof_lines:
forward reaction
Raw SKiD Env: Temp=-----, pH=-----


source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0


organism: Thermotoga maritima


enzyme_display_name: mRNA 5'-phosphatase

organism: Vaccinia virus (strain Western Reserve)

substrate_name: GpppA

proof_lines: A-570 and A-763 mutants have 3-fold and 10-fold higher Km values for GpppA.

enzyme: mRNA 5'-phosphatase

substrate: GpppA


substrate_name: acetoacetyl-CoA

substrate: acetoacetyl-CoA


organism: Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15)

substrate_name: cyclohexane carboxylic acid (with succinyl-CoA as CoA donor)


organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

substrate_name: pppA-poly(A)

substrate: pppA-poly(A)


organism: Semliki forest virus

substrate_name: nsP2 RNA triphosphatase activity (at pH 8.0)


organism: Venezuelan equine encephalitis virus (strain 3880)

substrate_name: a 12 residues substrate for nsp2 protease activity


organism: Venezuelan equine encephalitis virus (strain Trinidad donkey)

substrate: a 12 residues substrate for nsp2 protease activity


organism: Rhizobium meliloti (strain 1021)

substrate_name: FMN

assay_conditions_summary: cofactor FMN

proof_lines: kcat is 15 h(-1), 7.2 h(-1) and 7.6 h(-1) for FMN, NAD and NADP, respectively.

substrate: FMN


organism: Sinorhizobium meliloti

substrate_name: NADPH

assay_conditions_summary: pH 7.5; cofactor NADPH

substrate: NADPH


organism: Haloferax volcanii

substrate_name: ATP

assay_conditions_summary: pH 8; 315.15 K; cofactor ATP

substrate: ATP


organism: Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)

substrate_name: KDGal

sequence_source: UniProtKB D4GR05 reviewed-entry scalar metadata

substrate: KDGal


enzyme_display_name: diadenylate cyclase

organism: Bacillus subtilis (strain 168)

assay_conditions_summary: cofactor NADH

enzyme: diadenylate cyclase


organism: Mycolicibacterium smegmatis

assay_conditions_summary: pH 5.4; 310.15 K; cofactor ATP


enzyme_display_name: phenylsuccinyl-CoA transferase

organism: Aromatoleum aromaticum (strain DSM 19018 / LMG 30748 / EbN1)

substrate_name: Unknown

proof_lines: kcat is 0.178 sec(-1) with (R/S)-phenylsuccinate as substrate. kcat is 0.092 sec(-1) with (R)-benzylsuccinate as substrate. kcat is 0.064 sec(-1) with (S)-benzylsuccinate as substrate. kcat is 0.078 sec(-1) with (R/S)-benzylsuccinate as sub

enzyme: phenylsuccinyl-CoA transferase

substrate: Unknown


organism: Aromatoleum aromaticum

substrate_name: (R/S)-phenylsuccinyl-CoA

assay_conditions_summary: pH 8; cofactor CoA

proof_lines: at pH 8.0, temperature not specified in the publication

substrate: (R/S)-phenylsuccinyl-CoA


enzyme_display_name: endo-chitodextinase

organism: Vibrio furnissii

enzyme: endo-chitodextinase


enzyme_display_name: glucosylglycerate hydrolase

organism: Rubrobacter radiotolerans

enzyme: glucosylglycerate hydrolase


organism: Selaginella moellendorffii

sequence_source: UniProtKB:D8QTR2 (direct accession lookup; live reviewed entry with sequence present)


organism: Thermus thermophilus

sequence_source: UniProtKB:Q72K04 (direct accession lookup; live reviewed entry with sequence present)


enzyme_display_name: 4-nitrophenol 2-monooxygenase

organism: Oryctolagus cuniculus

assay_conditions_summary: pH 7.6; cofactor NADPH

proof_lines: reconstituted system of purified isozyme 3a, dilaroylglyceryl-3-phosphorylcholine and NADPH-cytochrome P-450 reductase at pH 7.6

enzyme: 4-nitrophenol 2-monooxygenase


organism: Homo sapiens

proof_lines: inhibition of chlorzoxazone 6-hydroxylation

proof_lines: inhibition of 4-nitrophenol hydroxylation


organism: Nocardia sp.


enzyme_display_name: desampylase

organism: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

substrate_name: SAMP2 dimer (with PfJAMM1 in the dimeric form, at 100 degrees Celsius)

proof_lines: kcat is 0.28 min(-1) for the cleavage of SAMP2 dimer using PfJAMM1 in the dimeric form. kcat is 0.16 min(-1) for the cleavage of SAMP2 dimer using PfJAMM1 in the monomeric form (at 100 degrees Celsius). kcat is 0.0016 min(-1) for the cleava

enzyme: desampylase


organism: Pyrococcus furiosus

substrate_name: SAMP2 dimer; H2O

substrate: SAMP2 dimer; H2O


enzyme_display_name: brachyurin

organism: Gadus morhua

enzyme: brachyurin


organism: Leptuca pugilator

substrate_name: N-benzoyl-L-Arg-p-nitroanilide

substrate: N-benzoyl-L-Arg-p-nitroanilide


enzyme_display_name: dTDP-epi-vancosaminyltransferase

organism: Amycolatopsis orientalis

substrate_name: desvancosaminyl-vancomycin

proof_lines:
pH 7.5, 37°C
Raw SKiD Env: Temp=37°C, pH=7.5

enzyme: dTDP-epi-vancosaminyltransferase

substrate: desvancosaminyl-vancomycin


enzyme_display_name: tRNA-guanine(15) transglycosylase

organism: Methanosarcina acetivorans

substrate_name: guanine15 in tRNA

proof_lines:
recombinant enzyme, at pH 7.5 and 37°C
native enzyme, at pH 7.5 and 37°C

enzyme: tRNA-guanine(15) transglycosylase

substrate: guanine15 in tRNA


enzyme_display_name: fatty acid photodecarboxylase

organism: Chlorella variabilis

substrate_name: palmitate

enzyme: fatty acid photodecarboxylase

substrate: palmitate


substrate_name: elaidic acid

substrate: elaidic acid


mutation_signature: truncated enzyme form FAP2 / truncated enzyme form FAP2

proof_lines: substrate presented in microemulsion, truncated enzyme form FAP2, at pH 9.0 and 20°C

proof_lines: substrate presented in large unilamellar vesicles, truncated enzyme form FAP2, at pH 9.0 and 20°C


organism: Escherichia coli

substrate_name: D-erythrose 4-phosphate

assay_conditions_summary: pH 7.5; 298.15 K; buffer MOPS; cofactor NAD+

proof_lines: wild type enzyme, in MOPS buffer (50 mM, pH 7.5), 0.050 mM CoCl2, 0.010 mM NAD+, D-erythrose 4-phosphate, 2 mM pyruvate, 1 unit of 3-dehydroquinate synthase, 1 unit of 3-dehydroquinate dehydratase, at 25°C

substrate: D-erythrose 4-phosphate


proof_lines: mutant enzyme F33I/D58N/Q72H/A75V/V85A/V154F, in MOPS buffer (50 mM, pH 7.5), 0.050 mM CoCl2, 0.010 mM NAD+, D-erythrose 4-phosphate, 2 mM pyruvate, 1 unit of 3-dehydroquinate synthase, 1 unit of 3-dehydroquinate dehydratase, at 25°C


organism: Roseateles saccharophilus


substrate_name: tRNA-Asp

substrate: tRNA-Asp


organism: Mycolicibacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 / 3849)

substrate_name: glucosylglycerate (at 37 degrees Celsius, C-terminally tagged variant)


organism: Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828 / KAW 2/3)

substrate_name: KDG (at 60 degrees Celsius and pH 6.2)

substrate: KDG (at 60 degrees Celsius and pH 6.2)


enzyme_display_name: hapalindole-type alkaloid chlorinase

organism: Fischerella ambigua

substrate_name: ambiguine C

proof_lines: pH and temperature not specified in the publication

enzyme: hapalindole-type alkaloid chlorinase

substrate: ambiguine C


organism: Hapalosiphon welwitschii

substrate_name: 12-epi-hapalindole C

substrate: 12-epi-hapalindole C


organism: Penicillium glabrum


enzyme_display_name: nitric oxide reductase (menaquinol)

organism: Pyrobaculum aerophilum

proof_lines:
54.5°C, pH 7.0, purified qNOR
54.5°C, pH 7.0, qNOR in membrane

enzyme: nitric oxide reductase (menaquinol)


organism: Schinkia azotoformans

substrate_name: nitric oxide

proof_lines: using plumbagin as electron donor, pH and temperature not specified in the publication

proof_lines: using cytochrome c551 as electron donor, pH and temperature not specified in the publication

proof_lines: using menaquinol as electron donor, pH and temperature not specified in the publication

substrate: nitric oxide


organism: Methanohalophilus portucalensis

substrate_name: glycine

substrate: glycine


organism: Thalassiosira pseudonana

substrate_name: N,N-dimethylglycine

substrate: N,N-dimethylglycine


proof_lines: truncation mutant carrying the N-terminal part, pH 8.0, 25°C


proof_lines: truncation mutant carrying the C-terminal part, pH 8.0, 25°C


enzyme_display_name: small RNA 2'-O-methyltransferase

organism: Acetivibrio thermocellus

substrate_name: [24mer sRNA]-3'-end ribonucleotide

enzyme: small RNA 2'-O-methyltransferase

substrate: [24mer sRNA]-3'-end ribonucleotide


organism: Arabidopsis thaliana


proof_lines: above 0.02 mM, C-terminal domain, pH 7.5, 37°C


enzyme_display_name: isobutyraldoxime O-methyltransferase

organism: Pseudomonas sp.

enzyme: isobutyraldoxime O-methyltransferase


enzyme_display_name: (phenol)carboxyphthiodiolenone synthase

organism: Mycobacterium tuberculosis

substrate_name: (S)-methylmalonyl-CoA

enzyme: (phenol)carboxyphthiodiolenone synthase

substrate: (S)-methylmalonyl-CoA


enzyme_display_name: lysine N-acetyltransferase

organism: Bos taurus

substrate_name: acetyl phosphate

proof_lines:
cosubstrate L-ornithine
cosubstrate L-lysine

enzyme: lysine N-acetyltransferase

substrate: acetyl phosphate


organism: Salmonella enterica

substrate_name: Acs (AMP forming)

assay_conditions_summary: pH 7; 310.15 K; cofactor CoA

substrate: Acs (AMP forming)


enzyme_display_name: lipopolysaccharide core heptose(I) kinase

organism: Escherichia coli

substrate_name: lipopolysaccharide acceptor

assay_conditions_summary: cofactor ATP

substrate: lipopolysaccharide acceptor


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

substrate_name: ATP

proof_lines: kcat is 27.23 min(-1) for lipopolysaccharide kinase activity.

enzyme: lipopolysaccharide core heptose(I) kinase

substrate: ATP


organism: Escherichia coli (strain K12)

substrate_name: KDPGal

proof_lines: kcat is 4 sec(-1) with KDPGal as substrate.

substrate: KDPGal


assay_conditions_summary: pH 7.5; 308.15 K; cofactor ATP


organism: Pseudomonas aeruginosa

assay_conditions_summary: pH 7.5; 310.15 K; cofactor ATP


enzyme_display_name: muramoyltetrapeptide carboxypeptidase

organism: Drosophila melanogaster

enzyme: muramoyltetrapeptide carboxypeptidase


organism: Novosphingobium aromaticivorans


substrate_name: UDP-N-acetylmuramyl-Ala-D-Glu-Dap-D-Ala

substrate: UDP-N-acetylmuramyl-Ala-D-Glu-Dap-D-Ala


organism: Lysinibacillus sphaericus


organism: Proteus vulgaris


enzyme_display_name: tropomyosin kinase

organism: Gallus gallus

substrate_name: alpha-tropomyosin

proof_lines: 37°C, pH 7.5, skeletal alpha-tropomyosin

enzyme: tropomyosin kinase

substrate: alpha-tropomyosin


enzyme_display_name: thermopsin

organism: Sulfolobus acidocaldarius

enzyme: thermopsin


enzyme_display_name: beta-porphyranase

organism: Chondrus crispus

substrate_name: Porphyran

enzyme: beta-porphyranase

substrate: Porphyran


organism: Wenyingzhuangia fucanilytica


organism: Phocaeicola plebeius

proof_lines: dockerin fusion chimeric enzyme, pH and temperature not specified in the publication


enzyme_display_name: N-acetylglucosamine deacetylase

substrate_name: N-acetyl-D-glucosamine

enzyme: N-acetylglucosamine deacetylase

substrate: N-acetyl-D-glucosamine


organism: Cyclobacterium marinum

proof_lines: pH 8.0, 42°C, recombinant wild-type dimeric enzyme


proof_lines: pH 8.0, 42°C, recombinant K219E mutant enzyme


organism: Thermococcus kodakarensis


enzyme_display_name: alkylamidase

organism: Ovis aries aries

substrate_name: N-Butylcaproamide

enzyme: alkylamidase

substrate: N-Butylcaproamide


enzyme_display_name: phthalyl amidase

organism: Xanthobacter agilis

enzyme: phthalyl amidase


substrate_name: o-phthalyl carbacephem

substrate: o-phthalyl carbacephem


enzyme_display_name: cysteine-S-conjugate beta-lyase

organism: Escherichia coli

substrate_name: L-Cystathionine

enzyme: cysteine-S-conjugate beta-lyase

substrate: L-Cystathionine


substrate_name: L-cystathionine

proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W188F/W230F/W300F/W340F

substrate: L-cystathionine


proof_lines: pH 8.5, 25°C, recombinant mutant W188F/W230F/W276F/W300F/W340F


proof_lines: pH 8.5, 25°C, recombinant wild-type enzyme


proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W230F/W276F/W300F


proof_lines: pH 8.5, 25°C, recombinant mutant W131FF/W230FF/W276F


organism: Candidatus Pelagibacter communis

substrate_name: cystathionine

proof_lines: recombinant MBP-tagged enzyme, pH 8.0, 37°C

substrate: cystathionine


organism: Candidatus Pelagibacter ubique


proof_lines: pH 8.5, 25°C, recombinant chimeric mutant enzyme eCGS-sC-eCBL

proof_lines: pH 8.5, 25°C, recombinant chimeric mutant enzyme eCGS-sN-eCBL


proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W188F/W230F/W276F/W300F/W340F


proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W188F/W230F/W276F


organism: Spinacia oleracea


organism: Wolbachia pipientis

substrate_name: H2O; L-Cystathionine

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C

substrate: H2O; L-Cystathionine


organism: Lacticaseibacillus casei

substrate_name: L-cysteine

assay_conditions_summary: pH 5.5; 310.15 K; buffer sodium phosphate

proof_lines: Ctl2, in sodium phosphate buffer, at pH 6.8 and 37°C

proof_lines: Ctl2, in sodium phosphate buffer, at pH 5.5 and 37°C

substrate: L-cysteine


organism: Latilactobacillus sakei

substrate_name: L-Cysteine

substrate: L-Cysteine


proof_lines: pH 10.0, 35°C, recombinant His-tagged enzyme


organism: Mimosa pudica


organism: Pelagibacter ubique


organism: Rattus norvegicus

substrate_name: S-(1,2-Dichlorovinyl)-L-cysteine; H2O

substrate: S-(1,2-Dichlorovinyl)-L-cysteine; H2O


organism: Saccharomyces cerevisiae

substrate_name: L-djenkolate

assay_conditions_summary: pH 8.5; 310.15 K; buffer 50 mM phosphate

proof_lines: in 50 mM phosphate buffer, pH 8.5, 0.02 mM pyridoxal 5-phosphate, 1 mM EDTA, at 37°C

substrate: L-djenkolate


organism: Thermotoga maritima

assay_conditions_summary: pH 8; 310.15 K; buffer recombinant His-tagged enzyme

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C, bicine buffer

proof_lines: recombinant His-tagged enzyme, pH 8.0, 37°C, Tris buffer

proof_lines: recombinant His-tagged enzyme, pH 8.0, 70°C, bicine buffer


organism: Arabidopsis thaliana

substrate_name: L-cystine

substrate: L-cystine


organism: Bacillus sp. (in: firmicutes)

substrate_name: L-selenodjenkolate

substrate: L-selenodjenkolate


organism: Bacillus subtilis


organism: Bordetella avium


organism: Nicotiana tabacum

substrate_name: UDPglucose

substrate: UDPglucose


organism: Paracoccus denitrificans


organism: Pseudomonas sp. 'cruciviae'

substrate_name: pyridoxal phosphate

substrate: pyridoxal phosphate


proof_lines: mitochondria cytoplasm


organism: Salmonella enterica


organism: Brassica oleracea


organism: Cavia porcellus


organism: Escherichia coli


organism: Homo sapiens

substrate_name: beta-chloro-L-alanine

substrate: beta-chloro-L-alanine


organism: Salmonella enterica subsp. enterica serovar Typhimurium


organism: Staphylococcus haemolyticus

proof_lines: pH 8.5, 37°C, purified recombinant enzyme

proof_lines: pH 8.0, 37°C, purified recombinant enzyme


organism: Synechocystis sp. PCC 6714

substrate_name: cystine

proof_lines: estimated from steady-state measurements

substrate: cystine


organism: Vachellia farnesiana


enzyme_display_name: cellulose synthase (UDP-forming)

organism: Oryza sativa

enzyme: cellulose synthase (UDP-forming)


organism: Populus tremula x Populus tremuloides

substrate_name: UDP-alpha-D-glucose

substrate: UDP-alpha-D-glucose


organism: Gossypium hirsutum


organism: Hordeum vulgare


proof_lines: pH 7.5, 35°C, depending on individual membrane preparation


organism: Novacetimonas hansenii


organism: Eubacterium limosum

substrate_name: L-carnitine

sequence_source: uniprot

substrate: L-carnitine


enzyme_display_name: acetyl-CoA C-myristoyltransferase

organism: Rattus norvegicus

substrate_name: acetoacetyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: acetyl-CoA C-myristoyltransferase

substrate: acetoacetyl-CoA


proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W230F/W276F/W340F


proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W188F/W230F/W276F/W300F


substrate_name: L-Cystathionine

substrate: L-Cystathionine


substrate_name: L-cystathionine

proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W230F/W276F/W300F/W340F

substrate: L-cystathionine


proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W188F/W230F/W276F/W340F


proof_lines: pH 8.5, 25°C, recombinant mutant W131F/W188F/W276F/W300F/W340F


enzyme_display_name: tricin synthase

substrate_name: S-Adenosyl-L-methionine; Tricetin

enzyme: tricin synthase

substrate: S-Adenosyl-L-methionine; Tricetin


enzyme_display_name: alpha-amino-acid esterase

substrate_name: 5'-D-Valyl-floxuridine; H2O

enzyme: alpha-amino-acid esterase

substrate: 5'-D-Valyl-floxuridine; H2O


enzyme_display_name: beta-amylase

organism: Bacillus cereus

substrate_name: H2O; Maltopentaose

enzyme: beta-amylase

substrate: H2O; Maltopentaose


proof_lines: pH 8.5, 25°C, recombinant mutants W340F and W131F/W230F/W276F/W340F


substrate_name: tricetin

substrate: tricetin


enzyme_display_name: sphingomyelin synthase

organism: Mus musculus

enzyme: sphingomyelin synthase


substrate_name: sphingomyelin

substrate: sphingomyelin


enzyme_display_name: terephthalate 1,2-dioxygenase

organism: Comamonas sp

substrate_name: TPA (at 30 degrees Celsius and at ph 7)

assay_conditions_summary: pH 7

enzyme: terephthalate 1,2-dioxygenase

substrate: TPA (at 30 degrees Celsius and at ph 7)


enzyme_display_name: sterigmatocystin 8-O-methyltransferase

organism: Aspergillus parasiticus (strain ATCC 56775 / NRRL 5862 / SRRC 143 / SU-1)

substrate_name: sterigmatocystin

enzyme: sterigmatocystin 8-O-methyltransferase

substrate: sterigmatocystin


organism: Papaver somniferum


organism: Oryza sativa subsp. japonica


enzyme_display_name: docosahexaenoic acid omega-hydroxylase

organism: Homo sapiens

substrate_name: docosanoate

proof_lines: kcat is 0.067 min(-1) with menaquinone-4 (MK-4) as substrate (PubMed:24138531). The omega-hydroxylation of VLCFAs follows dual-enzyme Michaelis-Menten kinetics, suggesting simultaneous binding of two substrate molecules. The high affinity M

enzyme: docosahexaenoic acid omega-hydroxylase

substrate: docosanoate


substrate_name: myristic acid

proof_lines: Vmax is nearly the same for myristic acid and for lauric acid and reduced about 30% for palmitic acid.

substrate: myristic acid


substrate_name: hexadecanoic acid

proof_lines: kcat is 0.088 min(-1) with menaquinone-4 (MK-4) as substrate (PubMed:24138531). kcat is 1.8 min(-1) with hexadecanoic acid as substrate. kcat is 1.0 min(-1) with (9Z)-octadecenoic acid as substrate. kcat is 0.54 min(-1) with (5Z,8Z,11Z,14Z)

substrate: hexadecanoic acid


substrate_name: leukotriene B4

proof_lines: The omega-hydroxylation of VLCFAs follows dual-enzyme Michaelis-Menten kinetics, suggesting simultaneous binding of two substrate molecules. The high affinity Michaelis-Menten constants are shown.

substrate: leukotriene B4


enzyme_display_name: pantetheine hydrolase

organism: Equus caballus

substrate_name: pantetheine

enzyme: pantetheine hydrolase

substrate: pantetheine


proof_lines: apparent Km value, soluble recombinant vanin-1, pH 7.7, 25°C

proof_lines: apparent Km value, HPLC assay, pH 7.7, 25°C


organism: Sus scrofa

substrate_name: (R)-pantetheine

substrate: (R)-pantetheine


enzyme_display_name: tyrosine isonitrile desaturase

organism: Erwinia amylovora (strain CFBP1430)

substrate_name: tyrosine isonitrile

enzyme: tyrosine isonitrile desaturase

substrate: tyrosine isonitrile


organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)


substrate_name: Unknown

substrate: Unknown


organism: Erwinia amylovora


proof_lines: isoform SMS2, in 50 mM Tris–HCl (pH 7.4), at 22°C


proof_lines: isoform SMS1, in 50 mM Tris–HCl (pH 7.4), at 22°C


substrate_name: 3-Chloro-L-alanine; H2O

substrate: 3-Chloro-L-alanine; H2O


organism: Lacticaseibacillus casei

substrate_name: L-cysteine

proof_lines: Ctl1, in sodium phosphate buffer, at pH 6.8 and 37°C

proof_lines: Ctl1, in sodium phosphate buffer, at pH 5.5 and 37°C

substrate: L-cysteine


enzyme_display_name: D-proline dehydrogenase

organism: Unknown

substrate_name: D-proline

enzyme: D-proline dehydrogenase

substrate: D-proline


organism: Pyrobaculum islandicum

proof_lines: Ketjen Black/D-Pro dehydrogenase electrode

proof_lines: carbon nanotube gel/D-Pro dehydrogenase electrode


enzyme_display_name: bacillolysin

organism: Bacillus subtilis

substrate_name: Z-Phe-Leu-Ala-OH; H2O

enzyme: bacillolysin

substrate: Z-Phe-Leu-Ala-OH; H2O


organism: Hordeum vulgare


enzyme_display_name: GDP-mannose 3,5-epimerase

organism: Arabidopsis thaliana

substrate_name: GDP-D-mannose

proof_lines: recombinant enzyme with N-terminal glutathione-S-transferase tag

proof_lines: recombinant enzyme with N-terminal His-tag

enzyme: GDP-mannose 3,5-epimerase

substrate: GDP-D-mannose


organism: Auxenochlorella pyrenoidosa

proof_lines: pH and temperature not specified in the publication


organism: Oryza sativa

substrate_name: GDP-alpha-D-mannose

substrate: GDP-alpha-D-mannose


assay_conditions_summary: cofactor NAD+

proof_lines:
in presence of 0.02 mM NAD+
without NAD+


enzyme_display_name: prenylcysteine oxidase

substrate_name: free farnesylcysteine

proof_lines: kcat is 8000 sec(-1) for free farnesylcysteine.

enzyme: prenylcysteine oxidase

substrate: free farnesylcysteine


organism: Bos taurus


substrate_name: geranylgeranylcysteine

substrate: geranylgeranylcysteine


substrate_name: farnesyl-L-cysteine

substrate: farnesyl-L-cysteine


substrate_name: beta-carotene

proof_lines: pH 7.0, temperature not specified in the publication

proof_lines: recombinant enzyme, pH not specified in the publication, 28°C

substrate: beta-carotene


proof_lines: addition of methanol to in vitro assays has a strong stimulatory effect.

data_origin: experimental


organism: Lathyrus oleraceus


enzyme_display_name: corticosterone 18-monooxygenase

substrate_name: corticosterone

proof_lines: pH 7.4, 37°C. The production of 18-OH corticosterone from corticosterone cannot be isolated from the production of aldosterone; therefore, the value is not true a steady-state parameter because the data reports on both reactions simultaneou

enzyme: corticosterone 18-monooxygenase

substrate: corticosterone


enzyme_display_name: cystine reductase

substrate_name: L-cystine

enzyme: cystine reductase

substrate: L-cystine


organism: Histoplasma capsulatum


organism: Saccharomyces cerevisiae


organism: Homo sapiens

substrate_name: lactosylceramide

substrate: lactosylceramide


organism: Oryctolagus cuniculus


organism: Rattus norvegicus

substrate_name: UDP-galactose

substrate: UDP-galactose


substrate_name: CMP-N-acetylneuraminate

substrate: CMP-N-acetylneuraminate


substrate_name: sialyl lactoetraosylceramide

proof_lines:
isoform ST6GalNAc V, pH 6.0, 37°C
isoform ST6GalNAc VI, pH 6.0, 37°C

substrate: sialyl lactoetraosylceramide


organism: Lancefieldella rimae

proof_lines: pH 7.8, temperature not specified in the publication


organism: Treponema denticola


organism: Mesorhizobium loti


organism: Pseudomonas sp.


enzyme_display_name: homogentisate solanesyltransferase

substrate_name: farnesyl diphosphate

proof_lines: with respect to farnesyl diphosphate, at pH 8.0 and 32°C

proof_lines: with respect to homogentisate, at pH 8.0 and 32°C

enzyme: homogentisate solanesyltransferase

substrate: farnesyl diphosphate


organism: Chlamydomonas reinhardtii

substrate_name: homogentisate

substrate: homogentisate


enzyme_display_name: galacturonokinase

substrate_name: D-galacturonate

enzyme: galacturonokinase

substrate: D-galacturonate


substrate_name: ATP

substrate: ATP


enzyme_display_name: 4-sulfomuconolactone hydrolase

organism: Agrobacterium tumefaciens

sequence_source: UniProtKB:A6XIG7 direct accession anchor; sequence is present in the live entry but was not imported into this row.

assay_conditions_summary: pH 8

proof_lines: pH 8.0, temperature not specified in the publication

enzyme: 4-sulfomuconolactone hydrolase


organism: Hydrogenophaga intermedia

sequence_source: UniProtKB:A6XIG6 direct accession anchor; sequence is present in the live entry but was not imported into this row.


organism: Lancefieldella rimae (strain ATCC 49626 / DSM 7090 / CCUG 31168 / NBRC 15546 / VPI D140H-11A)

sequence_source: Trusted canonical sequence is preserved in the selected source record for UniProt B9CM12; not emitted in this review.

proof_lines: kcat is 3.7 sec(-1) with 2-aminoethylphosphonate as substrate. kcat is 0.7 sec(-1) with phosphocholine as substrate.


organism: Treponema denticola (strain ATCC 35405 / DSM 14222 / CIP 103919 / JCM 8153 / KCTC 15104)

substrate_name: 2-aminoethylphosphonate (for cytidylyltransferase activity)

sequence_source: Trusted canonical sequence is preserved in the selected source record for UniProt Q73MU2; not emitted in this review.

proof_lines: kcat is 1.05 sec(-1) with 2-aminoethylphosphonate as substrate for cytidylyltransferase activity.


organism: Agrobacterium radiobacter

substrate_name: 4-sulfomuconolactone (at pH 8)

sequence_source: Trusted canonical sequence is preserved in the selected source record for UniProt A6XIG7; not emitted in this review.

proof_lines: kcat is 400 min(-1) with 4-sulfomuconolactone as substrate (at pH 8).

substrate: 4-sulfomuconolactone (at pH 8)


sequence_source: Trusted canonical sequence is preserved in the selected source record for UniProt A6XIG6; not emitted in this review.

proof_lines: kcat is 2700 min(-1) with 4-sulfomuconolactone (at pH 8).


enzyme_display_name: sulfite dehydrogenase (quinone)

organism: Aquifex aeolicus

substrate_name: sulfite

proof_lines: pH 8.3, 55°C, with Nitro-blue tetrazolium

enzyme: sulfite dehydrogenase (quinone)

substrate: sulfite


enzyme_display_name: carboxypeptidase Taq

organism: Trypanosoma cruzi

proof_lines:
in the presence of Co2+
in the absence of Co2+

enzyme: carboxypeptidase Taq


organism: Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

verification_status: corrected

proof_lines: kcat is 307.3 min(-1) for 2-(acetamidomethylene)succinate.

status: corrected


enzyme_display_name: streptothricin hydrolase

organism: Streptomyces noursei

substrate_name: streptothricin-F

enzyme: streptothricin hydrolase

substrate: streptothricin-F


enzyme_display_name: NAD(+) 3'-kinase

organism: Xanthomonas oryzae pv. oryzicola

substrate_name: NaAD+

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NAD+

proof_lines: with NAD+ as cosubstrate, at pH 7.5 and 25°C

proof_lines: with NAAD+ as cosubstrate, at pH 7.5 and 25°C

enzyme: NAD(+) 3'-kinase

substrate: NaAD+


organism: Bos taurus


organism: Homo sapiens


substrate_name: ganglioside GM1b

substrate: ganglioside GM1b


substrate_name: homogentisate (in the presence of 200 uM farnesyl diphosphate)


organism: Arabidopsis thaliana


enzyme_display_name: protein S-acyltransferase

organism: Rattus norvegicus

substrate_name: L-glutamate (with 2 mM of Mn(2+))

enzyme: protein S-acyltransferase

substrate: L-glutamate (with 2 mM of Mn(2+))


organism: Mus musculus

substrate_name: palmitoyl-CoA

assay_conditions_summary: pH 7.4; 298.15 K; cofactor CoA

substrate: palmitoyl-CoA


organism: Neurospora crassa

assay_conditions_summary: pH 7.2; buffer 50 mM phosphate


enzyme_display_name: salicylate 5-hydroxylase

organism: Ralstonia sp.

substrate_name: salicylate

assay_conditions_summary: pH 6.4; 295.15 K; buffer 50 mM MES; cofactor NADPH

enzyme: salicylate 5-hydroxylase

substrate: salicylate


proof_lines: 4°C, pH not specified in the publication

proof_lines: 23°C, pH not specified in the publication


enzyme_display_name: 3-hydroxyphenylacetate 6-hydroxylase

organism: Flavobacterium sp.

assay_conditions_summary: cofactor NADPH

enzyme: 3-hydroxyphenylacetate 6-hydroxylase


organism: Hedera helix

substrate_name: stearoyl-[acyl-carrier protein]

proof_lines: pH and temperature not specified in the publication

substrate: stearoyl-[acyl-carrier protein]


enzyme_display_name: ecdysone 20-monooxygenase

organism: Locusta migratoria migratorioides

substrate_name: ecdysone

enzyme: ecdysone 20-monooxygenase

substrate: ecdysone


organism: Manduca sexta

proof_lines:
mitochondrial enzyme system
microsomal enzyme
mitochondrial enzyme


organism: Schistocerca gregaria

substrate_name: alpha-ecdysone

substrate: alpha-ecdysone


organism: Spinacia oleracea


substrate_name: UDP-glucuronic acid

substrate: UDP-glucuronic acid


substrate_name: chondroitin sulfate undecasaccharide

substrate: chondroitin sulfate undecasaccharide


substrate_name: C11-oligosaccharide of chondroitin sulfate A


enzyme_display_name: isovaleryl-homoserine lactone synthase

organism: Bradyrhizobium diazoefficiens

substrate_name: isovaleryl-ACP

assay_conditions_summary: pH 7.3; cofactor CoA

proof_lines: pH 7.3, temperature not specified in the publication

enzyme: isovaleryl-homoserine lactone synthase

substrate: isovaleryl-ACP


organism: Acinetobacter calcoaceticus

proof_lines: with 1,2-dipalmitoyl-rac-glycerol as cosubstrate, pH and temperature not specified in the publication

proof_lines: with 1-hexadecanol as cosubstrate, pH and temperature not specified in the publication


organism: Marinobacter nauticus

substrate_name: n-Hexanol

sequence_source: UniProtKB:A1TX06 direct accession lookup (canonical entry only)

proof_lines: mutant enzyme M405F, pH and temperature not specified in the publication

substrate: n-Hexanol


proof_lines: mutant enzyme L356F, pH and temperature not specified in the publication


proof_lines: wild type enzyme, pH and temperature not specified in the publication


enzyme_display_name: 3-oxoglutarate synthase

organism: Atropa belladonna

substrate_name: malonyl-CoA

enzyme: 3-oxoglutarate synthase

substrate: malonyl-CoA


enzyme_display_name: galactan endo-beta-1,3-galactanase

organism: Flammulina velutipes

sequence_source: UniProtKB:F7J1C8 (reviewed entry; sequence present)

enzyme: galactan endo-beta-1,3-galactanase


proof_lines: kcat is 7.56 sec(-1) with beta-1,3-galactan. kcat is 5.47 sec(-1) with beta-1,3-galactotriose. kcat is 6.50 sec(-1) with beta-1,3-galactotetraose.


enzyme_display_name: limonoid glucosyltransferase

organism: Citrus maxima

substrate_name: UDP-glucose

proof_lines: pH 7.8, 37°C, enzyme immobilized covalently on cellulose carbonate

proof_lines: pH 7.8, 37°C, enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde

enzyme: limonoid glucosyltransferase

substrate: UDP-glucose


organism: Citrus maxima x Citrus paradisi

substrate_name: limonin

substrate: limonin


enzyme_display_name: beta-diketone hydrolase

organism: Sphingopyxis sp. (strain 113P3)

substrate_name: polyvinyl alcohol

enzyme: beta-diketone hydrolase

substrate: polyvinyl alcohol


organism: Sphingomonas sp.

substrate_name: p-nitrophenyl acetate

substrate: p-nitrophenyl acetate


enzyme_display_name: delphinidin 3',5'-O-glucosyltransferase

organism: Clitoria ternatea

proof_lines: delphinidin 3-O-(6-O-malonyl)-beta-D-glucoside-3-O-beta-glucoside as substrate

proof_lines: delphinidin 3-O-(6-O-malonyl)-beta-D-glucoside as substrate

enzyme: delphinidin 3',5'-O-glucosyltransferase


organism: Nostoc sp. PCC 7120 = FACHB-418


organism: Canis lupus familiaris


organism: Cavia porcellus


organism: Gallus gallus


organism: Mesocricetus auratus

substrate_name: UDP-N-acetyl-D-galactosamine

substrate: UDP-N-acetyl-D-galactosamine


organism: Lactococcus lactis


organism: Escherichia coli


substrate_name: MKATKLVLGAVILGSTLLAGCSSN

substrate: MKATKLVLGAVILGSTLLAGCSSN


enzyme_display_name: photosystem I

organism: Spinacia oleracea

substrate_name: Unknown

enzyme: photosystem I

substrate: Unknown


organism: Synechocystis sp.

substrate_name: reduced plastocyanin

substrate: reduced plastocyanin


enzyme_display_name: opheline kinase

organism: Ophelia neglecta

substrate_name: phosphotaurocyamine

assay_conditions_summary: 303.15 K; cofactor ATP

enzyme: opheline kinase

substrate: phosphotaurocyamine


enzyme_display_name: beta-glucogallin O-galloyltransferase

organism: Quercus robur

enzyme: beta-glucogallin O-galloyltransferase


organism: Arabidopsis thaliana


organism: Flaveria bidentis

substrate_name: quercetin 3,3'-disulfate

proof_lines: cosubstrate quercetin 3,3-disulfate, isoenzyme II

substrate: quercetin 3,3'-disulfate


organism: Dictyostelium discoideum

substrate_name: UDP-N-acetyl-D-glucosamine

substrate: UDP-N-acetyl-D-glucosamine


organism: Mus musculus

substrate_name: [D-glucuronic acid-N-acetyl-glucosamine]4 -D-glucuronic acid-2,5-anhydro-D-mannose


enzyme_display_name: N-sulfoglucosamine sulfohydrolase

organism: Homo sapiens

substrate_name: heparan sulfate

proof_lines: substrates deriving from heparan sulfate, unamplified recombinant enzyme

proof_lines: substrates deriving from heparan sulfate, gene-amplified recombinant enzyme

enzyme: N-sulfoglucosamine sulfohydrolase

substrate: heparan sulfate


organism: Pedobacter heparinus

substrate_name: Glucosamine 2,6-disulfate

substrate: Glucosamine 2,6-disulfate


organism: Rattus norvegicus

substrate_name: heparin

substrate: heparin


enzyme_display_name: L-arabinokinase

substrate_name: L-arabinose

enzyme: L-arabinokinase

substrate: L-arabinose


substrate_name: ATP

assay_conditions_summary: pH 8; 303.15 K; cofactor ATP

substrate: ATP


assay_conditions_summary: pH 6.5; 308.15 K; cofactor ATP


enzyme_display_name: scutelarin

organism: Bothrops cotiara

substrate_name: prothrombin

proof_lines:
22°C, pH 7.4, presence of S2238
22°C, pH 7.4

enzyme: scutelarin

substrate: prothrombin


organism: Oxyuranus scutellatus


enzyme_display_name: thiocyanate hydrolase

organism: Thiobacillus thioparus

substrate_name: thiocyanate

enzyme: thiocyanate hydrolase

substrate: thiocyanate


organism: Thiohalophilus thiocyanatoxydans


substrate_name: Methacrylonitrile

substrate: Methacrylonitrile


enzyme_display_name: citryl-CoA lyase

organism: Hydrogenobacter thermophilus

substrate_name: citryl-CoA

assay_conditions_summary: pH 8; 343.15 K; cofactor CoA

enzyme: citryl-CoA lyase

substrate: citryl-CoA


organism: Escherichia coli

substrate_name: acetyl-CoA

substrate: acetyl-CoA


substrate_name: oxaloacetate

substrate: oxaloacetate


enzyme_display_name: glutamyl aminopeptidase

organism: Bitis gabonica rhinoceros

substrate_name: L-Glutamic acid gamma-(7-amido-4-methylcoumarin); H2O

enzyme: glutamyl aminopeptidase


organism: Sus scrofa

substrate_name: Glu-p-nitroanilide; H2O

substrate: Glu-p-nitroanilide; H2O


enzyme_display_name: jasmonoyl--L-amino acid ligase

substrate_name: L-Isoleucine; Jasmonic acid; ATP

assay_conditions_summary: cofactor ATP

enzyme: jasmonoyl--L-amino acid ligase

substrate: L-Isoleucine; Jasmonic acid; ATP


substrate_name: Jasmonic acid; Glutamine; ATP

substrate: Jasmonic acid; Glutamine; ATP


enzyme_display_name: adenosylhomocysteinase

organism: Synechocystis sp. PCC 6803

substrate_name: S-adenosyl-L-homocysteine

sequence_source: UniProtKB direct accession lookup

enzyme: adenosylhomocysteinase

substrate: S-adenosyl-L-homocysteine


organism: Thermotoga maritima

substrate_name: adenosine

proof_lines:
at pH 6.5 and 75°C
Raw SKiD Env: Temp=75°C, pH=6.5

substrate: adenosine


organism: Trypanosoma cruzi


organism: Branchiostoma belcheri tsingtauense

substrate_name: L-homocysteine

substrate: L-homocysteine


organism: Corynebacterium efficiens

substrate_name: DL-homocysteine

substrate: DL-homocysteine


organism: Cryptosporidium parvum

substrate_name: S-adenosyl-DL-homocysteine

substrate: S-adenosyl-DL-homocysteine


organism: Cytophaga hutchinsonii

proof_lines:
pH 7.5, 22°C
at pH 7.5 and 22°C


organism: Homo sapiens


proof_lines: recombinant wild type enzyme, at pH 7.2 and 37°C


proof_lines:
pH 6.5, 41°C
pH 7.5, 37°C
Raw SKiD Env: Temp=37°C, pH=7.2


organism: Plasmodium falciparum

substrate_name: Unknown

substrate: Unknown


organism: Pseudomonas aeruginosa


proof_lines: pH 7.5, 20°C, recombinant wild-type enzyme, in presence of Rb+

proof_lines: pH 7.5, 20°C, recombinant wild-type enzyme, in presence of Li+


proof_lines: pH 7.5, 20°C, recombinant mutant Q65A, in presence of K+


proof_lines: pH and temperature not specified in the publication


organism: Pyrococcus horikoshii

assay_conditions_summary: pH 6.5; 333.15 K; cofactor NAD+

proof_lines: in the presence of 0.5 mM NAD+, at pH 6.5 and 60°C

proof_lines: in the absence of NAD+, at pH 6.5 and 60°C


organism: Rattus norvegicus

assay_conditions_summary: pH 7.2; 295.15 K; buffer 50 mM potassium phosphate

proof_lines: at 22°C in 50 mM potassium phosphate buffer, at pH 7.2


proof_lines: pH 7.5, 20°C, wild-type enzyme, 100 mM Na+

proof_lines: pH 7.5, 20°C, wild-type enzyme, without monovalent metal ion

proof_lines: pH 7.5, 20°C, wild-type enzyme, 100 mM Rb+


proof_lines: pH 7.5, 20°C, mutant enzyme R24E, 100 mM K+


proof_lines:
liver enzyme
column-retained isozyme
flow-through isozyme


proof_lines:
30°C, pH 7.6, wild-type enzyme
wild-type


organism: Pyrococcus furiosus


organism: Mus musculus

proof_lines:
mutant Sahn 12
mutant Sahn 12 enzyme


organism: Saccharolobus solfataricus


mutation_signature: truncated recombinant enzyme truncated recombinant enzyme


organism: Lupinus luteus

substrate_name: cysteine

substrate: cysteine


organism: Mesocricetus auratus


organism: Acinetobacter calcoaceticus


organism: Alcaligenes faecalis


organism: Beta vulgaris


organism: Bos taurus

proof_lines:
no phosphate added
phosphate added


organism: Bradyrhizobium elkanii

proof_lines:
23°C, pH 7.0
at pH 8.0 and 25°C


organism: Corynebacterium glutamicum


organism: Cryphonectria parasitica


organism: Dictyostelium discoideum


organism: Mycobacterium tuberculosis


organism: Plasmodium falciparum


organism: Trichomonas vaginalis

proof_lines:
native enzyme
recombinant enzyme


organism: Trypanosoma cruzi


enzyme_display_name: alpha-L-rhamnosidase

organism: Aspergillus aculeatus

enzyme: alpha-L-rhamnosidase


substrate_name: p-nitrophenyl-alpha-L-rhamnopyranoside

substrate: p-nitrophenyl-alpha-L-rhamnopyranoside


organism: Aspergillus niger

substrate_name: 4-nitrophenyl alpha-L-rhamnopyranoside

substrate: 4-nitrophenyl alpha-L-rhamnopyranoside


organism: Aspergillus terreus


proof_lines: pH 6.5, 65°C, in presence of 0.6 M sorbitol


substrate_name: p-Nitrophenyl-alpha-L-rhamnopyranoside

substrate: p-Nitrophenyl-alpha-L-rhamnopyranoside


organism: Aspergillus tubingensis

substrate_name: naringin

substrate: naringin


organism: Bacillus sp. (in: firmicutes)


proof_lines: crystallized complex with interacting site analyzed


organism: Bacteroides thetaiotaomicron

proof_lines:
50°C, pH 6.5
Raw SKiD Env: Temp=50°C, pH=6.5


organism: Caldicellulosiruptor bescii

proof_lines: recombinant wild-type enzyme, pH 7.0, 80°C


organism: Dictyoglomus thermophilum


proof_lines:
pH 5.0, 37°C
Raw SKiD Env: Temp=37°C, pH=5


organism: Lactiplantibacillus plantarum


substrate_name: hesperidin

substrate: hesperidin


proof_lines: pH 7.0, 30°C, recombinant isozyme Ram1, calculated by BRENDA


organism: Novosphingobium sp. PP1Y


substrate_name: quercitrin

substrate: quercitrin


proof_lines: pH 6.9, temperature not specified in the publication


substrate_name: rutin

substrate: rutin


organism: Thermoclostridium stercorarium subsp. stercorarium


organism: Thermoclostridium stercorarium subsp. thermolacticum


organism: Thermomicrobia bacterium

proof_lines: pH 7.0, 50°C, recombinant RhmB, versus 4-nitrophenyl alpha-L-rhamnopyranoside


proof_lines: pH 7.5, 50°C, recombinant RhmA, versus 4-nitrophenyl alpha-L-rhamnopyranoside


organism: Thermotoga maritima


organism: Thermotoga neapolitana


organism: Alternaria sp. L1


organism: Acrostalagmus luteoalbus


organism: Alternaria alternata


organism: Aspergillus niger


organism: Aspergillus oryzae


substrate_name: neohesperidin

proof_lines: recombinant wild-type enzyme, pH 5.0, 50°C, calculated by BRENDA

substrate: neohesperidin


organism: Aspergillus terreus


organism: Bacteroides JY-6


organism: Fagopyrum esculentum


organism: Fusarium verticillioides

proof_lines: pH 10.5 (0.2M sodium phosphate buffer), 50°C


organism: Fusobacterium sp.


proof_lines: recombinant enzyme, pH 7.5, temperature not specified in the publication


organism: Ogataea angusta


organism: Sphingomonas paucimobilis


organism: Turbo cornutus

substrate_name: p-nitrophenyl alpha-L-rhamnoside

substrate: p-nitrophenyl alpha-L-rhamnoside


organism: Papiliotrema laurentii


organism: Penicillium decumbens

proof_lines: pH 6.7, 30°C, versus 4-nitrophenyl alpha-L-rhamnopyranoside


organism: Penicillium sp.

proof_lines:
soluble, narginase activity
immobilized, narginase activity


organism: Penicillium tardum

proof_lines: pH 5.0, 37Â°C, versus 4-nitrophenyl alpha-L-rhamnopyranoside


enzyme_display_name: methanethiol oxidase

organism: Thiobacillus thioparus

substrate_name: methanethiol

enzyme: methanethiol oxidase

substrate: methanethiol


enzyme_display_name: sucrose-phosphate synthase

organism: Anabaena sp.

substrate_name: UDP-glucose

proof_lines: SPS-II SPS-I

enzyme: sucrose-phosphate synthase

substrate: UDP-glucose


organism: Brassica napus


organism: Hordeum vulgare


organism: Ipomoea batatas

substrate_name: D-fructose 6-phosphate

substrate: D-fructose 6-phosphate


organism: Nicotiana tabacum

substrate_name: glutathione

substrate: glutathione


organism: Silene vulgaris

substrate_name: bimane-glutathione

substrate: bimane-glutathione


organism: Solanum lycopersicum


organism: Spinacia oleracea

proof_lines: in presence of 2.5 mM glucose 6-phosphate

proof_lines: in presence and absence of glucose 6-phosphate


organism: Synechocystis sp.

assay_conditions_summary: pH 8; cofactor ADP

proof_lines: pH 8.0, cosubstrate: UDP-glucose, enzyme expressed in Nicotiana tabacum

proof_lines: pH 8.0, cosubstrate: UDP-glucose, enzyme expressed in Escherichia coli

proof_lines: pH 8.0, enzyme expressed in Nicotiana tabacum


organism: Trifolium repens


organism: Triticum aestivum


organism: Lathyrus oleraceus

proof_lines: in presence of 0.5 mM glucose 6-phosphate


organism: Neltuma juliflora

proof_lines: Km is not effected by addition of 20 mM glucose 6-phosphate or 15 mM phosphate

proof_lines: in presence of 20 mM glucose 6-phosphate


organism: Oryza sativa


organism: Solanum tuberosum


organism: Silene cucubalus

substrate_name: Glutathione; [Glu(-Cys)]n-Gly

substrate: Glutathione; [Glu(-Cys)]n-Gly


organism: Saccharum officinarum

substrate_name: D-Fructose 6-phosphate; UDP-D-glucose

substrate: D-Fructose 6-phosphate; UDP-D-glucose


enzyme_display_name: rubredoxin--NAD(+) reductase

organism: Megalodesulfovibrio gigas

substrate_name: Desulfovibrio gigas rubredoxin

assay_conditions_summary: cofactor NADH

enzyme: rubredoxin--NAD(+) reductase

substrate: Desulfovibrio gigas rubredoxin


enzyme_display_name: phosphosulfolactate synthase

organism: Methanocaldococcus jannaschii

substrate_name: phosphoenolpyruvate

enzyme: phosphosulfolactate synthase

substrate: phosphoenolpyruvate


substrate_name: Phosphoenolpyruvate; Sulfite

substrate: Phosphoenolpyruvate; Sulfite


enzyme_display_name: isopiperitenol dehydrogenase

organism: Mentha x piperita

substrate_name: (+)-trans-isoperitenol

enzyme: isopiperitenol dehydrogenase

substrate: (+)-trans-isoperitenol


enzyme_display_name: lignostilbene alphabeta-dioxygenase

organism: Sphingomonas paucimobilis

proof_lines:
isozyme IV
isozyme I
pH 8.5, 30°C

enzyme: lignostilbene alphabeta-dioxygenase


organism: Clostridium acetobutylicum

substrate_name: NADH; H+; Oxidized rubredoxin

substrate: NADH; H+; Oxidized rubredoxin


organism: Homo sapiens

substrate_name: H2O; O2; Methanethiol

proof_lines: pH and temperature not specified in the publication

substrate: H2O; O2; Methanethiol


organism: Hyphomicrobium sp.


organism: Arabidopsis thaliana


organism: Euglena gracilis

proof_lines: in the presence of 0.02 mM GSH, at pH 8.0, temperature not specified in the publication

proof_lines: in the presence of 0.04 mM GSH, at pH 8.0, temperature not specified in the publication


organism: Methylobacillus flagellatus

proof_lines: pH 6.5, 45°C, recombinant His6-tagged enzyme, in presence of fructose 1,6-bisphosphate


organism: Pisum sativum

substrate_name: UDP; Sucrose

substrate: UDP; Sucrose


enzyme_display_name: cysteate synthase

organism: Methanosarcina acetivorans

substrate_name: Sulfite; O-Phospho-L-serine

enzyme: cysteate synthase

substrate: Sulfite; O-Phospho-L-serine


enzyme_display_name: lupanine 17-hydroxylase (cytochrome c)

organism: Pseudomonas sp.

substrate_name: sparteine

enzyme: lupanine 17-hydroxylase (cytochrome c)

substrate: sparteine


enzyme_display_name: trans-anol O-methyltransferase

organism: Pimpinella anisum

substrate_name: isoeugenol

proof_lines: pH 7.5, temperature not specified in the publication

enzyme: trans-anol O-methyltransferase

substrate: isoeugenol


organism: Anethum foeniculum

substrate_name: S-adenosyl-L-methionine

substrate: S-adenosyl-L-methionine


enzyme_display_name: inositol 1-methyltransferase

organism: Lathyrus oleraceus

enzyme: inositol 1-methyltransferase


substrate_name: S-Adenosyl-L-methionine; myo-Inositol

substrate: S-Adenosyl-L-methionine; myo-Inositol


organism: Unknown

substrate_name: GTP

substrate: GTP


enzyme_display_name: phloroisovalerophenone synthase

organism: Humulus lupulus

assay_conditions_summary: cofactor CoA

enzyme: phloroisovalerophenone synthase


substrate_name: isovaleryl-CoA

substrate: isovaleryl-CoA


organism: Artemia salina


organism: Artemia sp.


enzyme_display_name: deoxyribonuclease (pyrimidine dimer)

organism: Tequatrovirus T4

substrate_name: DNA 14mer containing a TT-dimer

enzyme: deoxyribonuclease (pyrimidine dimer)

substrate: DNA 14mer containing a TT-dimer


assay_conditions_summary: pH 7

proof_lines: pH 7.0, temperature not specified in the publication


organism: Mus musculus


organism: Bos taurus

substrate_name: UDP-galactose

substrate: UDP-galactose


organism: Homo sapiens

substrate_name: GlcNAc-B-S-pNP

substrate: GlcNAc-B-S-pNP


substrate_name: UDP-Gal

substrate: UDP-Gal


enzyme_display_name: nerylneryl diphosphate synthase

organism: Solanum lycopersicum

substrate_name: isopentenyl diphosphate

enzyme: nerylneryl diphosphate synthase

substrate: isopentenyl diphosphate


organism: Rhodocyclus tenuis

substrate_name: Sulfide

proof_lines: with sulfide as cosubstrate, pH and temperature not specified in the publication

proof_lines: with thiosulfate as cosubstrate, pH and temperature not specified in the publication

substrate: Sulfide


organism: Arabidopsis thaliana

substrate_name: O-acetyl-L-serine

substrate: O-acetyl-L-serine


organism: Glycine max

substrate_name: (-)-glycinol

substrate: (-)-glycinol


enzyme_display_name: naringenin 8-dimethylallyltransferase

organism: Sophora flavescens

substrate_name: dimethylallyl diphosphate

enzyme: naringenin 8-dimethylallyltransferase

substrate: dimethylallyl diphosphate


organism: Salmonella enterica subsp. enterica serovar Typhimurium

proof_lines: at pH 7.6, temperature not specified in the publication


proof_lines: using microsomes of cultured soybean cells


substrate_name: naringenin

substrate: naringenin


substrate_name: 3,3-dimethylallyl diphosphate

substrate: 3,3-dimethylallyl diphosphate


enzyme_display_name: dihydroxyphenylalanine transaminase

organism: Cavia porcellus

enzyme: dihydroxyphenylalanine transaminase


substrate_name: 2-Oxoglutarate; L-Dopa

substrate: 2-Oxoglutarate; L-Dopa


organism: Rattus norvegicus


enzyme_display_name: succinyl-CoA--L-malate CoA-transferase

organism: Chloroflexus aurantiacus

substrate_name: (S)-malate

assay_conditions_summary: 328.15 K; cofactor CoA

proof_lines: 55°C, pH not specified in the publication, recombinant enzyme

enzyme: succinyl-CoA--L-malate CoA-transferase

substrate: (S)-malate


substrate_name: succinyl-CoA

substrate: succinyl-CoA


substrate_name: phosphatidylinositol 3,5-biphosphate

substrate: phosphatidylinositol 3,5-biphosphate


organism: Bifidobacterium longum subsp. Longum

proof_lines:
pH 5.5 and 45Â°C
at pH 5.5 and 45Â°C


organism: Mycolicibacterium hassiacum

proof_lines:
at pH 6.5 and 45°C
at pH 6.5 and 37°C


enzyme_display_name: UDP-sulfoquinovose synthase

organism: Arabidopsis sp.

substrate_name: UDP-glucose

enzyme: UDP-sulfoquinovose synthase

substrate: UDP-glucose


enzyme_display_name: aminodeoxychorismate lyase

substrate_name: D-aspartate

enzyme: aminodeoxychorismate lyase

substrate: D-aspartate


organism: Mycobacterium tuberculosis

sequence_source: UniProt Q79FW0 supports canonical identity only; no row-specific variant sequence was established.


sequence_source: UniProt Q79FW0 supports canonical identity only; selected row sequence remains unresolved.


enzyme_display_name: phosphogluconate dehydrogenase [NAD(P)(+)-dependent, decarboxylating]

organism: Pseudomonas sp.

substrate_name: NADP+

assay_conditions_summary: cofactor NADP+

proof_lines: using NADP+ as cosubstrate, pH and temperature not specified in the publication

substrate: NADP+


organism: Thermotoga maritima

assay_conditions_summary: pH 7.5; 353.15 K; buffer 50 mM HEPES; cofactor NADP+

proof_lines: with NADP+ as cosubstrate, in 50 mM HEPES buffer (pH 7.5), at 80°C

enzyme: phosphogluconate dehydrogenase [NAD(P)(+)-dependent, decarboxylating]


organism: Ovis aries


organism: Sus scrofa

substrate_name: N-acetyl-D-galactosaminyl-alpha-benzyl

substrate: N-acetyl-D-galactosaminyl-alpha-benzyl


enzyme_display_name: apigenin 4'-O-methyltransferase

organism: Robinia pseudoacacia

substrate_name: apigenin

enzyme: apigenin 4'-O-methyltransferase

substrate: apigenin


enzyme_display_name: glucosamine N-acetyltransferase

organism: Avena sativa

substrate_name: D-glucosamine

proof_lines:
CHAPS-solubilized enzyme
membrane-bound enzyme

enzyme: glucosamine N-acetyltransferase

substrate: D-glucosamine


organism: Clostridium acetobutylicum

substrate_name: acetyl-CoA

assay_conditions_summary: pH 7.3; 298.15 K; cofactor CoA

substrate: acetyl-CoA


substrate_name: ganglioside GD3

substrate: ganglioside GD3


organism: Equus caballus


organism: Mus musculus

substrate_name: UDP-N-acetyl-D-glucosamine

proof_lines: mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C

substrate: UDP-N-acetyl-D-glucosamine


organism: Homo sapiens

substrate_name: UDP-N-acetyl-D-galactosamine

substrate: UDP-N-acetyl-D-galactosamine


substrate_name: UDP-N-acetylgalactosamine

substrate: UDP-N-acetylgalactosamine


organism: Dictyostelium discoideum

proof_lines:
cosubstrate Man9GlcNAc
i.e. Man9GlcNAc, intersected product
intersected product


enzyme_display_name: Transferred entry: 2.4.3.1

substrate_name: Lactosamine; CMP-N-acetylneuraminate

enzyme: Transferred entry: 2.4.3.1

substrate: Lactosamine; CMP-N-acetylneuraminate


organism: Rattus norvegicus

substrate_name: Fetuin; CMP-N-acetylneuraminate

substrate: Fetuin; CMP-N-acetylneuraminate


enzyme_display_name: Transferred entry: 2.4.3.7

substrate_name: CMP-N-acetylneuraminate; Fetuin

enzyme: Transferred entry: 2.4.3.7

substrate: CMP-N-acetylneuraminate; Fetuin


organism: Bacillus subtilis

substrate_name: L-glutamate

proof_lines: pH not specified in the publication, temperature not specified in the publication

substrate: L-glutamate


organism: Stenotrophomonas maltophilia

substrate_name: chorismate

proof_lines: second reaction step, cosubstrate NH3, pH 8, 25°C

substrate: chorismate


enzyme_display_name: hygromycin B 4-O-kinase

organism: Escherichia coli

substrate_name: ATP

proof_lines:
HPH
HPH and HPH5
HPH5

enzyme: hygromycin B 4-O-kinase

substrate: ATP


enzyme_display_name: receptor protein serine/threonine kinase

substrate_name: KKVLTQMGSPSIRCS(P)SVS

assay_conditions_summary: pH 7.5; 303.15 K; cofactor ATP

proof_lines: pH 7.5, 30°C, recombinant wild-type ALK5

substrate: KKVLTQMGSPSIRCS(P)SVS


proof_lines: pH 7.5, 30°C, recombinant mutant T204D ALK5, inhibition of autophosphorylation

enzyme: receptor protein serine/threonine kinase


enzyme_display_name: protein-histidine pros-kinase

proof_lines: in presence of activators: GTP or recombinant RAS protein

proof_lines: in absence of activators: GTP or recombinant RAS protein

enzyme: protein-histidine pros-kinase


enzyme_display_name: branched-chain-fatty-acid kinase

organism: Spirochaeta sp.

substrate_name: valerate

enzyme: branched-chain-fatty-acid kinase

substrate: valerate


organism: Unknown

substrate_name: carboxyphosphoenolpyruvate

substrate: carboxyphosphoenolpyruvate


organism: Streptomyces hygroscopicus


enzyme_display_name: propionate CoA-transferase

organism: Anaerotignum propionicum

substrate_name: propionate

enzyme: propionate CoA-transferase

substrate: propionate


assay_conditions_summary: pH 7; 298.15 K; cofactor CoA


organism: Cupriavidus necator

substrate_name: propionyl-CoA

assay_conditions_summary: pH 7.4; 303.15 K; cofactor CoA

substrate: propionyl-CoA


enzyme_display_name: pApA phosphodiesterase

organism: Streptococcus mitis

enzyme: pApA phosphodiesterase


organism: Streptococcus pneumoniae D39


enzyme_display_name: [protein ADP-ribosylarginine] hydrolase

substrate_name: ADP-ribosylarginine

assay_conditions_summary: cofactor ADP

enzyme: [protein ADP-ribosylarginine] hydrolase

substrate: ADP-ribosylarginine


organism: Meleagris gallopavo


substrate_name: ADP-ribosylarginine methyl ester

proof_lines: in presence of 15 mM magnesium and 5 mM DTT

substrate: ADP-ribosylarginine methyl ester


organism: Lysinibacillus sphaericus

proof_lines:
enzyme from spores
enzyme from sporulation medium
immobilized enzyme


proof_lines:
pH 7.3, 37°C, recombinant enzyme
pH 7.3, 37°C


enzyme_display_name: arginyltransferase

organism: Oryctolagus cuniculus

substrate_name: Unknown

enzyme: arginyltransferase

substrate: Unknown


organism: Saccharomyces cerevisiae

substrate_name: L-Arginyl-tRNA

substrate: L-Arginyl-tRNA


organism: Sus scrofa


enzyme_display_name: isopullulanase

organism: Aspergillus niger

substrate_name: pullulan

enzyme: isopullulanase

substrate: pullulan


substrate_name: panose

proof_lines:
mutant enzyme W402F
pH 3.5, 40°C, mutant enzyme W402F

substrate: panose


proof_lines:
wild-type enzyme
pH 3.5, 40°C, wild-type enzyme


enzyme_display_name: L-peptidase

organism: Foot-and-mouth disease virus

enzyme: L-peptidase


proof_lines: isoform Lbpro, pH and temperature not specified in the publication


enzyme_display_name: scytalidopepsin B

organism: Scytalidium lignicola

proof_lines: pH and temperature not specified in the publication

enzyme: scytalidopepsin B


enzyme_display_name: N-methyl-2-oxoglutaramate hydrolase

organism: Pseudomonas sp.

substrate_name: alpha-ketoglutarate

proof_lines: turnover number based on the molecular weight of 90000

enzyme: N-methyl-2-oxoglutaramate hydrolase

substrate: alpha-ketoglutarate


enzyme_display_name: arylalkyl acylamidase

organism: Pseudomonas putida

enzyme: arylalkyl acylamidase


enzyme_display_name: L-threonine aldolase

organism: Clostridium pasteurianum

substrate_name: L-threonine

enzyme: L-threonine aldolase

substrate: L-threonine


organism: Escherichia coli

substrate_name: L-allo-threonine

substrate: L-allo-threonine


enzyme_display_name: envelysin

organism: Paracentrotus lividus

substrate_name: dimethylated casein

enzyme: envelysin

substrate: dimethylated casein


organism: Strongylocentrotus purpuratus


enzyme_display_name: cyclohexanone monooxygenase

organism: Acidovorax sp. CHX100

substrate_name: cyclohexanone

assay_conditions_summary: cofactor NADPH

proof_lines: suspended cells, pH not specified in the publication, temperature not specified in the publication

proof_lines: biofilm, pH not specified in the publication, temperature not specified in the publication

proof_lines: purified enzyme, pH not specified in the publication, temperature not specified in the publication

enzyme: cyclohexanone monooxygenase

substrate: cyclohexanone


organism: Acinetobacter johnsonii

assay_conditions_summary: pH 8.5; 293.15 K; cofactor NADPH

proof_lines:
pH 8.5, 20°C, wild-type enzyme
25°C, pH 8.0, wild-type enzyme


organism: Acinetobacter sp.


substrate_name: NADPH

assay_conditions_summary: pH 7.5; 298.15 K; cofactor NADPH

proof_lines:
wild-type, pH 7.7, 25°C
wild-type, pH 7.5, 25°C

substrate: NADPH


assay_conditions_summary: pH 8; cofactor NADPH

proof_lines: recombinant engineered enzyme expressed in Escherichia coli, pH 8.0, temperature not specified in the publication

proof_lines: recombinant engineered enzyme expressed in Pichia pastoris, pH 8.0, temperature not specified in the publication