sequence_source: UniProtKB Q97UH8 canonical FASTA, fetched 2026-05-31


enzyme_display_name: beta-glucosidase

organism: Saccharolobus solfataricus

substrate_name: N,N'-diacetyl-beta-chitobiose

source_db: oed

verification_status: unverified

ki_display: Not reported

mutation_signature: Unknown

protein_accession_database: UniProt

kcat_over_km_origin: calculated

source_license: CC BY 4.0

km_unit: mM

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

data_origin: experimental

enzyme: beta-glucosidase

substrate: N,N'-diacetyl-beta-chitobiose

status: unverified

source: oed


substrate_name: 4-nitrophenyl beta-D-glucoside

substrate: 4-nitrophenyl beta-D-glucoside


organism: Secale cereale

substrate_name: p-Nitrophenyl-beta-D-glucoside

sequence_source: UniProtKB Q9FYS3 canonical FASTA with PMID 16751439 mutation signature reconstructed on full precursor coordinates, fetched 2026-05-31

sequence_variant_note: Mutation signature S465L could not be applied safely because the resolved sequence does not match the expected reference residue(s).

mutation_type: substitution

substrate: p-Nitrophenyl-beta-D-glucoside


sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset +9, so it is treated as a source-provided variant sequence.


sequence_variant_note: Mutation signature G464S/S465L could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature Y378F could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature Y378A could not be applied safely because the resolved sequence does not match the expected reference residue(s).


kcat_over_km_display: Not reported

sequence_source: UniProtKB:Q9FYS3 exact accession lookup via UniProt REST

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9FYS3 exact accession lookup via UniProt REST and applied mutation signature G464S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q9FYS3 exact accession lookup via UniProt REST and applied mutation signature G464F to reconstruct the assayed variant sequence.


organism: Sorghum bicolor

substrate_name: Dhurrin

sequence_source: UniProtKB:Q41290 exact accession lookup via UniProt REST

substrate: Dhurrin


substrate_name: o-Nitrophenyl-beta-D-glucopyranoside

substrate: o-Nitrophenyl-beta-D-glucopyranoside


enzyme_display_name: Beta-glucosidase 8

enzyme_name_source: UniProtKB exact accession follow-up

organism: Oryza sativa

substrate_name: laminaribiose

sequence_source: UniProtKB reviewed accession Q75I94 exact lookup; fetched 2026-05-31

enzyme: Beta-glucosidase 8

substrate: laminaribiose


enzyme_display_name: Beta-glucosidase 26

sequence_source: UniProtKB reviewed accession A3BMZ5 exact lookup; fetched 2026-05-31

enzyme: Beta-glucosidase 26


substrate_name: cellohexaose

substrate: cellohexaose


substrate_name: Cellopentaose

substrate: Cellopentaose


enzyme_display_name: Beta-glucosidase 1A

organism: Phanerochaete chrysosporium

substrate_name: Cellobiose

sequence_source: UniProtKB reviewed accession Q25BW5 exact lookup; fetched 2026-05-31

enzyme: Beta-glucosidase 1A

substrate: Cellobiose


enzyme_display_name: Beta-glucosidase 1B

sequence_source: UniProtKB reviewed accession Q25BW4 exact lookup; fetched 2026-05-31

enzyme: Beta-glucosidase 1B


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


organism: Thermofilum pendens

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

sequence_source: UniProtKB REST accession A1S0B1 checked 2026-05-31

substrate: 4-nitrophenyl beta-D-glucopyranoside


substrate_name: cellobiose

substrate: cellobiose


organism: Thermoplasma acidophilum

sequence_source: UniProtKB REST accession Q9HIL6 checked 2026-05-31


organism: Halothermothrix orenii

substrate_name: lactose

verification_status: corrected

sequence_variant_note: Resolved canonical sequence from UniProtKB:B8CYA8 direct accession check on 2026-05-31 and applied mutation signature Y296F to reconstruct the assayed variant sequence.

substrate: lactose

status: corrected


sequence_source: UniProtKB:B8CYA8 direct accession check on 2026-05-31


sequence_variant_note: Resolved canonical sequence from UniProtKB:B8CYA8 direct accession check on 2026-05-31 and applied mutation signature N222F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B8CYA8 direct accession check on 2026-05-31 and applied mutation signature N294T to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B8CYA8 direct accession check on 2026-05-31 and applied mutation signature F417S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B8CYA8 direct accession check on 2026-05-31 and applied mutation signature F417Y to reconstruct the assayed variant sequence.


enzyme_display_name: Probable beta-galactosidase

sequence_source: UniProtKB TrEMBL accession Q9HIL6 exact lookup; fetched 2026-05-31

enzyme: Probable beta-galactosidase


enzyme_display_name: Beta-glycosidase

organism: Thermosphaera aggregans

substrate_name: 2-nitrophenyl beta-D-glucopyranoside

sequence_variant_note: Resolved canonical sequence from UniProtKB TrEMBL accession Q9YGA8 exact lookup; fetched 2026-05-31 plus direct residue substitution validation for N62Y/Q63K/L231F/V303A/E386G/A412V and applied mutation signature N62Y/Q63K/L231F/V303A/E386G/A412V to reconstruct the assayed variant sequence.

enzyme: Beta-glycosidase

substrate: 2-nitrophenyl beta-D-glucopyranoside


sequence_source: UniProtKB TrEMBL accession Q9YGA8 exact lookup; fetched 2026-05-31


enzyme_display_name: Beta-glucosidase

organism: Thermotoga neapolitana

sequence_variant_note: Resolved canonical sequence from UniProtKB TrEMBL accession Q0GC07 exact lookup; fetched 2026-05-31 plus direct residue substitution validation for D242G and applied mutation signature D242G to reconstruct the assayed variant sequence.

enzyme: Beta-glucosidase


sequence_source: UniProtKB TrEMBL accession Q0GC07 exact lookup; fetched 2026-05-31

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q0GC07; direct REST lookup 2026-05-31 and applied mutation signature W243F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q0GC07; direct REST lookup 2026-05-31 and applied mutation signature D58A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:Q0GC07; direct REST lookup 2026-05-31 and applied mutation signature E458G to reconstruct the assayed variant sequence.


substrate_name: quercetin 3-O-beta-D-glucopyranoside

sequence_source: UniProtKB:B9K7M5; direct REST lookup 2026-05-31

substrate: quercetin 3-O-beta-D-glucopyranoside


sequence_variant_note: Resolved canonical sequence from UniProtKB:B9K7M5; direct REST lookup 2026-05-31 and applied mutation signature F224I to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B9K7M5; direct REST lookup 2026-05-31 and applied mutation signature N220S to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:B9K7M5; direct REST lookup 2026-05-31 and applied mutation signature N221F to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:B9K7M5 direct REST check; UniProtKB B9K7M5 canonical residue N221 matched; variant reconstructed as N221S

sequence_variant_note: Mutation signature N221S matches the mutant residue at offset +0 and the reference residue at offset -1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:B9K7M5 direct REST check; UniProtKB B9K7M5 canonical residue N220 matched; variant reconstructed as N220F

sequence_variant_note: Mutation signature N220F matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:B9K7M5 direct REST check; wild-type canonical sequence from UniProtKB B9K7M5


sequence_source: UniProtKB:B9K7M5 direct REST check; UniProtKB B9K7M5 canonical residue F310 matched; variant reconstructed as F310E

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


sequence_source: UniProtKB:B9K7M5 direct REST check; UniProtKB B9K7M5 canonical residue F310 matched; variant reconstructed as F310L


sequence_source: UniProtKB:B9K7M5 direct REST check; UniProtKB B9K7M5 canonical residue W322 matched; variant reconstructed as W322A

sequence_variant_note: Mutation signature W322A matches the mutant residue at offset +0 and the reference residue at offset +9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:B9K7M5 direct REST check; UniProtKB B9K7M5 canonical residue N220 matched; variant reconstructed as N220S

sequence_variant_note: Mutation signature N220S matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


organism: Thermotoga petrophila

substrate_name: 4-nitrophenyl beta-D-xylopyranoside

sequence_source: UniProtKB:A5IL97 direct lookup on 2026-05-31; live TrEMBL entry A5IL97_THEP1, beta-glucosidase EC 3.2.1.21, Thermotoga petrophila strain RKU-1, CRC64 97D590DA3C511738.

substrate: 4-nitrophenyl beta-D-xylopyranoside


substrate_name: 4-nitrophenyl beta-D-cellobioside

substrate: 4-nitrophenyl beta-D-cellobioside


sequence_source: Candidate UniProtKB:D2C716 direct lookup on 2026-05-31 found a live TrEMBL GH3-domain protein from Thermotoga petrophila strain RKU-10, CRC64 CE85ED8A486BC09D, but it was not accepted as the EC 3.2.1.21 sequence.

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


substrate_name: 4-nitrophenyl beta-D-galactopyranoside

substrate: 4-nitrophenyl beta-D-galactopyranoside


organism: Triticum aestivum

sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.


sequence_source: UniProtKB:Q1XH05 canonical sequence from direct REST lookup


organism: uncultured microorganism

substrate_name: 4-nitrophenyl beta-D-fucopyranoside

substrate: 4-nitrophenyl beta-D-fucopyranoside


sequence_source: UniProtKB:A0A1E1FFN6 canonical sequence from direct REST lookup


sequence_source: UniProtKB REST direct accession check:A5IL43


substrate_name: 4-nitrophenyl alpha-D-fucopyranoside

sequence_source: UniProtKB REST direct accession check:D2C716

substrate: 4-nitrophenyl alpha-D-fucopyranoside


organism: Thermus thermophilus

sequence_source: UniProtKB REST direct accession check:Q0GA07


substrate_name: p-Nitrophenyl-beta-D-glucoside

sequence_source: UniProtKB REST direct accession check:Q1XH04

substrate: p-Nitrophenyl-beta-D-glucoside


sequence_source: UniProtKB REST direct accession check:Q1XIR9


organism: Zea mays


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature F193Y with inferred residue-numbering offset +2 to reconstruct the assayed variant sequence.


substrate_name: Laminaritriose

sequence_source: UniProtKB reviewed Q75I94/BGL08_ORYSJ direct REST lookup on 2026-05-31; sequence version 1; EC 3.2.1.21 and kinetics cite PMID 19766588.

substrate: Laminaritriose


sequence_source: UniProtKB reviewed A3BMZ5/BGL26_ORYSJ direct REST lookup on 2026-05-31; sequence version 1; EC 3.2.1.21 and kinetics cite PMID 19766588.


substrate_name: cellotriose

substrate: cellotriose


substrate_name: Cellotetraose

substrate: Cellotetraose


substrate_name: cellohexaose

substrate: cellohexaose


sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 canonical FASTA; mutation reconstructed using RCSB/UniProt mature-chain offset +54 and applied mutation signature F198V with inferred residue-numbering offset +54 to reconstruct the assayed variant sequence.


substrate_name: Dhurrin

sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 canonical FASTA; mutation reconstructed using RCSB/UniProt mature-chain offset +54 and applied mutation signature M263F with inferred residue-numbering offset +54 to reconstruct the assayed variant sequence.

substrate: Dhurrin


sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 canonical FASTA; mutation reconstructed using RCSB/UniProt mature-chain offset +54 and applied mutation signature F205L with inferred residue-numbering offset +54 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 canonical FASTA; mutation reconstructed using RCSB/UniProt mature-chain offset +54 and applied mutation signature Y473F with inferred residue-numbering offset +54 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 canonical FASTA; mutation reconstructed using RCSB/UniProt mature-chain offset +54 and applied mutation signature P377A with inferred residue-numbering offset +54 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 canonical FASTA; mutation reconstructed using RCSB/UniProt mature-chain offset +54 and applied mutation signature D261N with inferred residue-numbering offset +54 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 canonical FASTA; mutation reconstructed using RCSB/UniProt mature-chain offset +54 and applied mutation signature F198V/F205L/P377A with inferred residue-numbering offset +54 to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:P49235 canonical FASTA; mutation reconstructed using RCSB/UniProt mature-chain offset +54


substrate_name: o-Nitrophenyl-beta-D-glucopyranoside

sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 canonical sequence with F198V/F205L applied at mature-chain offset +54 and applied mutation signature F198V/F205L with inferred residue-numbering offset +54 to reconstruct the assayed variant sequence.

substrate: o-Nitrophenyl-beta-D-glucopyranoside


sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 canonical sequence with M263F applied at mature-chain offset +54 and applied mutation signature M263F with inferred residue-numbering offset +54 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 canonical sequence with F466S/A467S applied at mature-chain offset +54 and applied mutation signature F466S/A467S with inferred residue-numbering offset +54 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 canonical sequence with F466S applied at mature-chain offset +54 and applied mutation signature F466S with inferred residue-numbering offset +54 to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:P49235 canonical sequence with F466S applied at mature-chain offset +54


sequence_source: UniProtKB:P49235 canonical sequence with F198V/F205L applied at mature-chain offset +54


sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 canonical precursor sequence; F193I reconstructed by mapping PMID 11706179 Zm-p60.1 mature residue F193 to precursor residue 252 after the paper's stated Zm-p60.1/ZmGlu1 five-residue mature N-terminus offset. and applied mutation signature F193I with inferred residue-numbering offset +2 to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:P49235 canonical precursor sequence from direct REST/FASTA lookup.

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


sequence_source: UniProtKB:P49235 canonical precursor sequence; E186Q reconstructed by mapping PMID 11706179 Zm-p60.1 mature residue E186 to precursor residue 245 after the paper's stated Zm-p60.1/ZmGlu1 five-residue mature N-terminus offset.

sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset +15, so it is treated as a source-provided variant sequence.


sequence_source: UniProtKB:P49235 canonical precursor sequence; F198V/F205L reconstructed by mapping PMID 12684498 ZmGlu1 mature residues F198 and F205 to precursor residues 252 and 259 using the UniProt transit peptide ending at residue 54.

sequence_variant_note: Mutation signature F198V/F205L could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_source: UniProtKB:P49235 canonical precursor sequence; Y473F reconstructed by mapping PMID 12684498 ZmGlu1 mature residue Y473 to precursor residue 527 using the UniProt transit peptide ending at residue 54.

sequence_variant_note: Mutation signature Y473F could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_source: UniProtKB:P49235 canonical precursor sequence; F466S reconstructed by mapping PMID 12684498 ZmGlu1 mature residue F466 to precursor residue 520 using the UniProt transit peptide ending at residue 54.

sequence_variant_note: Mutation signature F466S could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_source: UniProtKB:P49235 canonical precursor sequence; F205L reconstructed by mapping PMID 12684498 ZmGlu1 mature residue F205 to precursor residue 259 using the UniProt transit peptide ending at residue 54.

sequence_variant_note: Mutation signature F205L could not be applied safely because the resolved sequence does not match the expected reference residue(s).


organism: Humicola insolens

sequence_source: UniProtKB:O93784 canonical sequence; A0A076JRL8 is a secondary accession; direct REST lookup 2026-05-31


substrate_name: 4-nitrophenyl beta-D-glucopyranoside

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Lamium galeobdolon

sequence_source: UniProtKB:A0A3G1VCB1 canonical sequence; selected from source bundle by LgGLU1/Bglu1 paper-table identity; direct REST lookup 2026-05-31


substrate_name: oleuropein

substrate: oleuropein


organism: Listeria innocua Clip11262

sequence_source: UniProtKB:Q92AS9 canonical sequence; direct REST lookup 2026-05-31


substrate_name: sophorotetraose

substrate: sophorotetraose


substrate_name: laminaribiose

sequence_variant_note: Resolved canonical sequence from UniProtKB:Q92AS9 canonical sequence with R572K reconstructed after confirming residue 572 is R; direct REST lookup 2026-05-31 and applied mutation signature R572K to reconstruct the assayed variant sequence.

substrate: laminaribiose


sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 REST check on 2026-05-31; canonical precursor sequence from reviewed Swiss-Prot entry HGGL1_MAIZE; mutation reconstructed with paper-to-UniProt residue offset +59 and applied mutation signature M258F with inferred residue-numbering offset +59 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 REST check on 2026-05-31; canonical precursor sequence from reviewed Swiss-Prot entry HGGL1_MAIZE; mutation reconstructed with paper-to-UniProt residue offset +59 and applied mutation signature E186D with inferred residue-numbering offset +59 to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:P49235 REST check on 2026-05-31; canonical precursor sequence from reviewed Swiss-Prot entry HGGL1_MAIZE


sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 REST check on 2026-05-31; canonical precursor sequence from reviewed Swiss-Prot entry HGGL1_MAIZE; mutation reconstructed with paper-to-UniProt residue offset +59 and applied mutation signature E401Q with inferred residue-numbering offset +59 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 REST check on 2026-05-31; canonical precursor sequence from reviewed Swiss-Prot entry HGGL1_MAIZE; mutation reconstructed with paper-to-UniProt residue offset +59 and applied mutation signature M258V with inferred residue-numbering offset +59 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 REST check on 2026-05-31; canonical precursor sequence from reviewed Swiss-Prot entry HGGL1_MAIZE; mutation reconstructed with paper-to-UniProt residue offset +59 and applied mutation signature F193W with inferred residue-numbering offset +59 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 REST check on 2026-05-31; canonical precursor sequence from reviewed Swiss-Prot entry HGGL1_MAIZE; mutation reconstructed with paper-to-UniProt residue offset +59 and applied mutation signature E401D with inferred residue-numbering offset +59 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P49235 REST check on 2026-05-31; canonical precursor sequence from reviewed Swiss-Prot entry HGGL1_MAIZE; mutation reconstructed with paper-to-UniProt residue offset +59 and applied mutation signature M258I with inferred residue-numbering offset +59 to reconstruct the assayed variant sequence.


enzyme_display_name: beta-galactosidase

organism: Arabidopsis thaliana

substrate_name: o-Nitrophenyl-beta-D-galactoside

sequence_source: canonical UniProtKB sequence from Q9SCV8

enzyme: beta-galactosidase

substrate: o-Nitrophenyl-beta-D-galactoside


substrate_name: p-Nitrophenyl-beta-D-galactoside

substrate: p-Nitrophenyl-beta-D-galactoside


organism: Arthrobacter sp.

substrate_name: lactose

sequence_source: canonical UniProtKB sequence from Q7WTU5

substrate: lactose


organism: Aspergillus oryzae

substrate_name: 2-nitrophenyl beta-D-galactoside

sequence_source: canonical UniProtKB sequence from Q2UCU3

substrate: 2-nitrophenyl beta-D-galactoside


sequence_source: UniProtKB Q2UCU3 canonical sequence with W806F reconstructed after confirming W at residue 806

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


substrate_name: 4-nitrophenyl beta-D-galactopyranoside

substrate: 4-nitrophenyl beta-D-galactopyranoside


organism: Escherichia coli

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


sequence_source: UniProt REST FASTA P00722; >sp|P00722|BGAL_ECOLI Beta-galactosidase OS=Escherichia coli (strain K12) OX=83333 GN=lacZ PE=1 SV=2


sequence_source: UniProt REST FASTA P06864; >sp|P06864|BGA2_ECOLI Evolved beta-galactosidase subunit alpha OS=Escherichia coli (strain K12) OX=83333 GN=ebgA PE=1 SV=4


sequence_source: UniProtKB P00722 checked directly; variant sequence not populated because H540 does not map directly to residue 540 in the current canonical sequence.


substrate_name: p-Nitrophenyl-beta-D-fucopyranoside

substrate: p-Nitrophenyl-beta-D-fucopyranoside


sequence_source: UniProtKB P00722 canonical sequence fetched from rest.uniprot.org on 2026-05-31; accession organism and EC matched the selected record.


substrate_name: Phenyl-beta-D-galactopyranoside

sequence_source: UniProtKB:P06864 direct REST/FASTA lookup on 2026-05-31

substrate: Phenyl-beta-D-galactopyranoside


sequence_source: UniProtKB:P00722 direct REST/FASTA lookup on 2026-05-31

assay_conditions_summary: pH 7; 298.15 K; buffer 30 mM TES

column_label: Table I (Kinetic and energetic values for the action of the wild type and the substituted enzymes on galactosyl substrates and on substrates that are similar to galactose but do not have C6 hydroxyl groups), PNPA substrate block, enzyme column 'WILD'; metric row 'kcat (s^-1)' = 211; metric row 'Km (mM)' = 11.6; metric row 'kcat/Km (s^-1 mM^-1)' = 18.2; metric row 'DDGS (kJ/mol)' prints no value in the WILD column.

normalized_value_unit_evidence: kcat: printed Table I cell 211 in the PNPA block, column 'WILD', under the metric row head kcat (s^-1); the row is already per second, so no conversion applies and the paper value is 211.0 s^(-1). Km: printed Table I cell 11.6 under the metric row head Km (mM); the row is already in mM, so no conversion applies and the paper value is 11.6 mM. kcat/Km: printed Table I cell 18.2 under the metric row head kcat/Km (s^-1 mM^-1); the row is already in s^-1 mM^-1, so no conversion applies and the paper value is 18.2 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table I, PNPA substrate block, enzyme column 'WILD': kcat = 211 s^-1; Km = 11.6 mM; printed kcat/Km = 18.2 s^-1 mM^-1.

row_label: Table I, PNPA block, WILD column

table_label: Table I: Kinetic and energetic values for the action of the wild type and the substituted enzymes on galactosyl substrates and on substrates that are similar to galactose but do not have C6 hydroxyl groups (J. Biol. Chem. 1996, 271(24):14296-14301)


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P00722 and applied mutation signature H540F with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.

column_label: Table I (Kinetic and energetic values for the action of the wild type and the substituted enzymes on galactosyl substrates and on substrates that are similar to galactose but do not have C6 hydroxyl groups), PNPG substrate block, enzyme column 'H540F'; metric row 'kcat (s^-1)' = 0.1; metric row 'Km (mM)' = 0.45; metric row 'kcat/Km (s^-1 mM^-1)' = 0.22; metric row 'DDGS (kJ/mol)' = -22.8.

normalized_value_unit_evidence: kcat: printed Table I cell 0.1 in the PNPG block, column 'H540F', under the metric row head kcat (s^-1); the row is already per second, so no conversion applies and the paper value is 0.1 s^(-1). Km: printed Table I cell 0.45 under the metric row head Km (mM); the row is already in mM, so no conversion applies and the paper value is 0.45 mM. kcat/Km: printed Table I cell 0.22 under the metric row head kcat/Km (s^-1 mM^-1); the row is already in s^-1 mM^-1, so no conversion applies and the paper value is 0.22 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table I, PNPG substrate block, enzyme column 'H540F': kcat = 0.1 s^-1; Km = 0.45 mM; printed kcat/Km = 0.22 s^-1 mM^-1.

row_label: Table I, PNPG block, H540F column


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

column_label: Table I (Kinetic and energetic values for the action of the wild type and the substituted enzymes on galactosyl substrates and on substrates that are similar to galactose but do not have C6 hydroxyl groups), PNPF substrate block, enzyme column 'WILD'; metric row 'kcat (s^-1)' = 123.4; metric row 'Km (mM)' = 16.9; metric row 'kcat/Km (s^-1 mM^-1)' = 7.3; metric row 'DDGS (kJ/mol)' prints no value in the WILD column.

normalized_value_unit_evidence: kcat: printed Table I cell 123.4 in the PNPF block, column 'WILD', under the metric row head kcat (s^-1); the row is already per second, so no conversion applies and the paper value is 123.4 s^(-1). Km: printed Table I cell 16.9 under the metric row head Km (mM); the row is already in mM, so no conversion applies and the paper value is 16.9 mM. kcat/Km: printed Table I cell 7.3 under the metric row head kcat/Km (s^-1 mM^-1); the row is already in s^-1 mM^-1, so no conversion applies and the paper value is 7.3 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table I, PNPF substrate block, enzyme column 'WILD': kcat = 123.4 s^-1; Km = 16.9 mM; printed kcat/Km = 7.3 s^-1 mM^-1.

row_label: Table I, PNPF block, WILD column


sequence_variant_note: Resolved canonical sequence from UniProtKB:P00722 direct REST/FASTA lookup on 2026-05-31 and applied mutation signature H540N with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P00722 direct REST/FASTA lookup on 2026-05-31 and applied mutation signature H540E with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


column_label: Table I (Kinetic and energetic values for the action of the wild type and the substituted enzymes on galactosyl substrates and on substrates that are similar to galactose but do not have C6 hydroxyl groups), PNPG substrate block, enzyme column 'WILD'; metric row 'kcat (s^-1)' = 90; metric row 'Km (mM)' = 0.041; metric row 'kcat/Km (s^-1 mM^-1)' = 2200; metric row 'DDGS (kJ/mol)' prints no value in the WILD column.

normalized_value_unit_evidence: kcat: printed Table I cell 90 in the PNPG block, column 'WILD', under the metric row head kcat (s^-1); the row is already per second, so no conversion applies and the paper value is 90.0 s^(-1). Km: printed Table I cell 0.041 under the metric row head Km (mM); the row is already in mM, so no conversion applies and the paper value is 0.041 mM. kcat/Km: printed Table I cell 2200 under the metric row head kcat/Km (s^-1 mM^-1); the row is already in s^-1 mM^-1, so no conversion applies and the paper value is 2200.0 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table I, PNPG substrate block, enzyme column 'WILD': kcat = 90 s^-1; Km = 0.041 mM; printed kcat/Km = 2200 s^-1 mM^-1.

row_label: Table I, PNPG block, WILD column


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P00722 and applied mutation signature H540N with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.

column_label: Table I (Kinetic and energetic values for the action of the wild type and the substituted enzymes on galactosyl substrates and on substrates that are similar to galactose but do not have C6 hydroxyl groups), PNPG substrate block, enzyme column 'H540N'; metric row 'kcat (s^-1)' = 0.3; metric row 'Km (mM)' = 0.069; metric row 'kcat/Km (s^-1 mM^-1)' = 4.4; metric row 'DDGS (kJ/mol)' = -15.4.

normalized_value_unit_evidence: kcat: printed Table I cell 0.3 in the PNPG block, column 'H540N', under the metric row head kcat (s^-1); the row is already per second, so no conversion applies and the paper value is 0.3 s^(-1). Km: printed Table I cell 0.069 under the metric row head Km (mM); the row is already in mM, so no conversion applies and the paper value is 0.069 mM. kcat/Km: printed Table I cell 4.4 under the metric row head kcat/Km (s^-1 mM^-1); the row is already in s^-1 mM^-1, so no conversion applies and the paper value is 4.4 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table I, PNPG substrate block, enzyme column 'H540N': kcat = 0.3 s^-1; Km = 0.069 mM; printed kcat/Km = 4.4 s^-1 mM^-1.

row_label: Table I, PNPG block, H540N column


sequence_variant_note: Resolved canonical sequence from UniProt:P00722 FASTA; H391K reconstructed from PMID 11310566 using mature-chain offset +1 and applied mutation signature H391K with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt:P00722 FASTA; W999L reconstructed from PMID 12578395 using mature-chain offset +1 and applied mutation signature W999L with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_source: UniProt:P00722 FASTA; W999L reconstructed from PMID 12578395 using mature-chain offset +1


sequence_variant_note: Resolved canonical sequence from UniProt:P00722 FASTA; W999G reconstructed from PMID 12578395 using mature-chain offset +1 and applied mutation signature W999G with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProt:P00722 FASTA; W999Y reconstructed from PMID 12578395 using mature-chain offset +1 and applied mutation signature W999Y with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_source: UniProt:P00722 FASTA; W999Y reconstructed from PMID 12578395 using mature-chain offset +1


sequence_source: UniProt:P00722 FASTA; W999G reconstructed from PMID 12578395 using mature-chain offset +1


sequence_variant_note: Resolved canonical sequence from UniProt:P00722 FASTA; W999F reconstructed from PMID 12578395 using mature-chain offset +1 and applied mutation signature W999F with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Canonical sequence is preserved, but mutation context is present; variant sequence was not attached without safely applying the mutation.


sequence_variant_note: Resolved canonical sequence from UniProtKB:P00722 REST canonical sequence; mutation applied with inferred residue-numbering offset +1 and applied mutation signature G794A with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:P00722 REST canonical sequence; mutation applied with inferred residue-numbering offset +1


sequence_variant_note: Resolved canonical sequence from UniProtKB:P00722 REST canonical sequence; mutation applied with inferred residue-numbering offset +1 and applied mutation signature E461H with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


verification_status: verified

sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P00722 and applied mutation signature E461D with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 7; 298.15 K; buffer 30 mM

column_label: Table I: Kinetic constants and rate constants obtained for the beta-galactosidases with ONPG and PNPG as substrates (Journal of Biological Chemistry 1990, volume 265, number 10, pages 5512-5518); the PNPG substrate block; column head 'ASD' is the Asp substitution, the E461D-beta-galactosidase enzyme; the k_cat stub of that block, rendered 'k,, (s-l)', holds 0.007 in that column; the 'Km (mM)' stub holds 0.011 in that column; the block carries no kcat/Km stub

normalized_value_unit_evidence: kcat: printed Table I cell 0.007 s^-1; the cell is already in s^-1, so no unit conversion applies; the exported value is 0.007 s^(-1). Km: printed Table I cell 0.011 mM; the cell is already in mM, so no unit conversion applies; the exported value is 0.011 mM. kcat/Km: this paper prints no efficiency cell anywhere, so the catalog value is typed as the same-measurement quotient of the two printed cells of this one column, 0.007 / 0.011 = 0.6363636363636365 s^-1 mM^-1, which is the exported value.

raw_value_unit_evidence: Table I, PNPG block, column headed ASD (Asp), the E461D-beta-galactosidase enzyme: the k_cat stub prints 0.007 with the block unit s^-1, and the Km stub prints 0.011 with the block unit mM. Table I prints no catalytic-efficiency column.

row_label: Table I, PNPG substrate block, the k_cat and Km stubs read across to the column headed ASD, which is the Asp substitution, the E461D-beta-galactosidase enzyme

status: verified


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P00722 and applied mutation signature E461K with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.

column_label: Table I: Kinetic constants and rate constants obtained for the beta-galactosidases with ONPG and PNPG as substrates (Journal of Biological Chemistry 1990, volume 265, number 10, pages 5512-5518); the PNPG substrate block; column head 'LYS' is the Lys substitution, the E461K-beta-galactosidase enzyme; the k_cat stub of that block, rendered 'k,, (s-l)', holds 0.24 in that column; the 'Km (mM)' stub holds 0.008 in that column; the block carries no kcat/Km stub

normalized_value_unit_evidence: kcat: printed Table I cell 0.24 s^-1; the cell is already in s^-1, so no unit conversion applies; the exported value is 0.24 s^(-1). Km: printed Table I cell 0.008 mM; the cell is already in mM, so no unit conversion applies; the exported value is 0.008 mM. kcat/Km: this paper prints no efficiency cell anywhere, so the catalog value is typed as the same-measurement quotient of the two printed cells of this one column, 0.24 / 0.008 = 30.0 s^-1 mM^-1, which is the exported value.

raw_value_unit_evidence: Table I, PNPG block, column headed LYS (Lys), the E461K-beta-galactosidase enzyme: the k_cat stub prints 0.24 with the block unit s^-1, and the Km stub prints 0.008 with the block unit mM. Table I prints no catalytic-efficiency column.

row_label: Table I, PNPG substrate block, the k_cat and Km stubs read across to the column headed LYS, which is the Lys substitution, the E461K-beta-galactosidase enzyme


sequence_variant_note: Resolved canonical sequence from UniProtKB:P00722 REST canonical sequence; mutation applied with inferred residue-numbering offset +1 and applied mutation signature E461G with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:P00722 and applied mutation signature E461Q with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.

column_label: Table I: Kinetic constants and rate constants obtained for the beta-galactosidases with ONPG and PNPG as substrates (Journal of Biological Chemistry 1990, volume 265, number 10, pages 5512-5518); the PNPG substrate block; column head 'Gin' is the Gln substitution, the E461Q-beta-galactosidase enzyme; the k_cat stub of that block, rendered 'k,, (s-l)', holds 0.34 in that column; the 'Km (mM)' stub holds 0.003 in that column; the block carries no kcat/Km stub

normalized_value_unit_evidence: kcat: printed Table I cell 0.34 s^-1; the cell is already in s^-1, so no unit conversion applies; the exported value is 0.34 s^(-1). Km: printed Table I cell 0.003 mM; the cell is already in mM, so no unit conversion applies; the exported value is 0.003 mM. kcat/Km: this paper prints no efficiency cell anywhere, so the catalog value is typed as the same-measurement quotient of the two printed cells of this one column, 0.34 / 0.003 = 113.33333333333334 s^-1 mM^-1, which is the exported value.

raw_value_unit_evidence: Table I, PNPG block, column headed Gin (Gln), the E461Q-beta-galactosidase enzyme: the k_cat stub prints 0.34 with the block unit s^-1, and the Km stub prints 0.003 with the block unit mM. Table I prints no catalytic-efficiency column.

row_label: Table I, PNPG substrate block, the k_cat and Km stubs read across to the column headed Gin, which is the Gln substitution, the E461Q-beta-galactosidase enzyme


sequence_source: UniProtKB:A0A1E1FFN6 direct REST/FASTA lookup on 2026-05-31


substrate_name: 4-nitrophenyl beta-D-fucopyranoside

substrate: 4-nitrophenyl beta-D-fucopyranoside


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


organism: Lactobacillus delbrueckii

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


sequence_source: UniProtKB:Q1G9Z4 plus E491A reconstruction from checked residue 491

sequence_variant_note: Mutation signature E491A matches the mutant residue at offset +0 and the reference residue at offset +15; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


organism: Putranjiva roxburghii

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


substrate_name: cellobiose

substrate: cellobiose


sequence_source: UniProtKB:A0A023MIF8 direct FASTA lookup


organism: Saccharolobus solfataricus

sequence_source: UniProtKB:P22498 direct FASTA lookup


sequence_source: UniProtKB:P22498 canonical sequence with F441Y substitution reconstructed after direct residue check

sequence_variant_note: Mutation signature F441Y matches the mutant residue at offset +0 and the reference residue at offset -4; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:P22498 canonical sequence with F359Q substitution reconstructed after direct residue check

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


organism: Sulfolobus solfataricus

substrate_name: Methyl beta-D-galactoside

column_label: Table 3, row Methyl beta-D-galactopyranoside: Km cell 23 under mM; kcat cell 242 under min^-1; kcat/Km cell 10.5 under min^-1 mM^-1.

raw_value_unit_evidence: Literal retained Table 3 cells: Km 23 mM; kcat 242 min^-1; kcat/Km 10.5 min^-1 mM^-1.

row_label: Methyl beta-D-galactopyranoside

table_label: Table 3

substrate: Methyl beta-D-galactoside


column_label: Table 3, row P-NpGal: Km cell 0.225 under mM; kcat cell 5700 under min^-1; kcat/Km cell 25333 under min^-1 mM^-1.

raw_value_unit_evidence: Literal retained Table 3 cells: Km 0.225 mM; kcat 5700 min^-1; kcat/Km 25333 min^-1 mM^-1.

row_label: P-NpGal


substrate_name: alpha-Lactose

column_label: Table 3, row alpha-Lactose: Km cell 13 under mM; kcat cell 114 under min^-1; kcat/Km cell 8.7 under min^-1 mM^-1.

raw_value_unit_evidence: Literal retained Table 3 cells: Km 13 mM; kcat 114 min^-1; kcat/Km 8.7 min^-1 mM^-1.

row_label: alpha-Lactose

substrate: alpha-Lactose


column_label: Table 3, row Phenyl beta-D-galactopyranoside: Km cell 2.6 under mM; kcat cell 166 under min^-1; kcat/Km cell 63.8 under min^-1 mM^-1.

raw_value_unit_evidence: Literal retained Table 3 cells: Km 2.6 mM; kcat 166 min^-1; kcat/Km 63.8 min^-1 mM^-1.

row_label: Phenyl beta-D-galactopyranoside


sequence_variant_note: Resolved canonical sequence from UniProtKB P00722 REST canonical sequence; selected mutation reconstructed with residue-numbering offset +1 and applied mutation signature H540E with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB P00722 REST canonical sequence; selected mutation reconstructed with residue-numbering offset +1 and applied mutation signature H357F with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_source: UniProtKB P00722 REST canonical sequence; selected mutation reconstructed with residue-numbering offset +1


sequence_variant_note: Resolved canonical sequence from UniProtKB P00722 REST canonical sequence; selected mutation reconstructed with residue-numbering offset +1 and applied mutation signature H357L with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB P00722 REST canonical sequence; selected mutation reconstructed with residue-numbering offset +1 and applied mutation signature H357D with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB P00722 REST canonical sequence; selected mutation reconstructed with residue-numbering offset +1 and applied mutation signature H357N with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


organism: Bacillus subtilis

sequence_source: UniProtKB O31529 canonical reviewed FASTA; direct REST TSV reports Beta-galactosidase YesZ, Bacillus subtilis strain 168, EC 3.2.1.23.


sequence_variant_note: Resolved canonical sequence from Reconstructed from UniProtKB O31529 canonical reviewed FASTA by applying E145A after direct residue check showed O31529 residue 145 is E; PMID 17485082 names the E145A YesZ mutant. and applied mutation signature E145A to reconstruct the assayed variant sequence.


organism: Bacteroides ovatus

sequence_source: UniProtKB A7LXS9 canonical reviewed FASTA; direct REST TSV reports Beta-galactosidase BoGH2A, Bacteroides ovatus strain ATCC 8483, EC 3.2.1.23.


sequence_source: UniProtKB P00722 was checked directly, but the row-specific variant sequence was not accepted because canonical P00722 residue 391 is S while residue 392 is H; the cited His-391 mutation needs explicit numbering/construct validation before reconstruction.


enzyme_display_name: laccase

organism: Bacillus pumilus

sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB A0A0G2QNK1 canonical FASTA fetched 2026-05-31 plus selected mutation_signature and applied mutation signature L386W/G417L/G57L to reconstruct the assayed variant sequence.

enzyme: laccase


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:A0A0G2QNK1 and applied mutation signature G417L to reconstruct the assayed variant sequence.

column_label: Table 1: Kinetic parameters for the wild-type CotA and mutants using ABTS as substrate (Appl Microbiol Biotechnol 2017, 101:1935-1944); printed column heads Enzyme | kcat (s-1) | Km (mM) | kcat/Km (s-1 mM-1). Table 1, body row 'G417L'; column kcat (s-1) = 51.67; column Km (mM) = 0.127; column kcat/Km (s-1 mM-1) = 406.82. captured primary segment: G417L | kcat 51.67 s-1 | Km 0.127 mM | kcat/Km 406.82 s-1 mM-1

normalized_value_unit_evidence: kcat: printed Table 1 cell 51.67 in a column headed kcat (s-1); s-1 is already the canonical s^(-1), so no unit conversion applies and the value is 51.67 s^(-1). Km: printed Table 1 cell 0.127 in a column headed Km (mM); mM is already canonical, so no unit conversion applies and the value is 0.127 mM. kcat/Km: printed Table 1 cell 406.82 in a column headed kcat/Km (s-1 mM-1); 1 s^-1 mM^-1 is 1 s^(-1)*mM^(-1), so no unit conversion applies and the value is 406.82 s^(-1)*mM^(-1). The exported values are kcat 51.67 s^(-1) and Km 0.127 mM and kcat_over_km 406.85039370078744 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 1, body row 'G417L': kcat = 51.67 s-1; Km = 0.127 mM; printed kcat/Km = 406.82 s-1 mM-1.

row_label: Table 1, body row 'G417L'


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB A0A0G2QNK1 canonical FASTA fetched 2026-05-31 plus selected mutation_signature and applied mutation signature L386W/G417L/D152Y to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB A0A0G2QNK1 canonical FASTA fetched 2026-05-31 plus selected mutation_signature and applied mutation signature L386W/G417L/D152F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB A0A0G2QNK1 canonical FASTA fetched 2026-05-31 plus selected mutation_signature and applied mutation signature L386W to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB A0A0G2QNK1 canonical FASTA fetched 2026-05-31 plus selected mutation_signature and applied mutation signature L386W/G417L/D195F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB A0A0G2QNK1 canonical FASTA fetched 2026-05-31 plus selected mutation_signature and applied mutation signature L386W/G417L/D195W to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from reconstructed from UniProtKB A0A0G2QNK1 canonical FASTA fetched 2026-05-31 plus selected mutation_signature and applied mutation signature L386W/G417L/G57W to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:A0A0G2QNK1; reconstructed_variant_sequence; direct lookup 2026-05-31


substrate_name: guaiacol

sequence_source: UniProtKB:K4PL78; reconstructed_variant_sequence; direct lookup 2026-05-31

substrate: guaiacol


sequence_source: UniProtKB:K4PL78; canonical_wild_type_sequence; direct lookup 2026-05-31

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


sequence_variant_note: Mutation signature L386Q/G417I matches the mutant residue at offset +0 and the reference residue at offset -35; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


organism: Bacillus licheniformis

substrate_name: Syringaldazine

sequence_source: UniProtKB REST A0A0A6ZHX8 FASTA fetched 2026-05-31

substrate: Syringaldazine


sequence_source: UniProtKB REST H8WGE4 FASTA fetched 2026-05-31; mutation_signature D500G applied after residue check


sequence_source: UniProtKB REST I3XLC2 FASTA fetched 2026-05-31


substrate_name: Guaiacol

sequence_source: UniProtKB REST I3RYX9 FASTA fetched 2026-05-31

substrate: Guaiacol


sequence_source: UniProtKB REST A0A0G2QNK1 FASTA fetched 2026-05-31; mutation_signature L386W/G417L applied after residue check

sequence_variant_note: Mutation signature L386W/G417L matches the mutant residue at offset +0 and the reference residue at offset -35; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB REST A0A0G2QNK1 FASTA fetched 2026-05-31


organism: Bacillus altitudinis

substrate_name: Sinapoylcholine

sequence_source: UniProtKB direct accession lookup:A0A193DSS0; retrieved 2026-05-31

substrate: Sinapoylcholine


substrate_name: sinapate

substrate: sinapate


organism: Bacillus amyloliquefaciens

sequence_source: UniProtKB direct accession lookup:A0A6C0GY11; retrieved 2026-05-31


sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


organism: Bacillus clausii

sequence_source: UniProtKB direct accession lookup:Q5WEM6; retrieved 2026-05-31


organism: Acidobacteriaceae


organism: Acinetobacter baumannii

sequence_source: UniProtKB:D0C997 canonical FASTA, checked 2026-05-31


substrate_name: catechol

substrate: catechol


organism: Aeromonas hydrophila

sequence_source: UniProtKB REST direct accession lookup: D5FL31, retrieved 2026-05-31


organism: Agrobacterium sp. S5-1

sequence_source: UniProtKB REST direct accession lookup: A0A0F6SIA9, retrieved 2026-05-31


organism: alpha proteobacterium Q-1


substrate_name: I-

substrate: I-


organism: Aquifex aeolicus

sequence_source: UniProtKB REST direct accession lookup: O67206, retrieved 2026-05-31


substrate_name: Cu+

substrate: Cu+


organism: Bacillus coagulans


sequence_source: UniProtKB:Q65MU7 direct REST lookup on 2026-05-31


sequence_source: UniProtKB:H8WGE4 direct REST lookup on 2026-05-31


substrate_name: Reactive black 5

sequence_source: UniProtKB:A0A0G2QNK1 direct accession lookup on 2026-05-31; reconstructed variant sequence for T415D after residue checks.

sequence_variant_note: Mutation signature T415D matches the mutant residue at offset +0 and the reference residue at offset +3; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

substrate: Reactive black 5


sequence_source: UniProtKB:A0A0G2QNK1 direct accession lookup on 2026-05-31; reconstructed variant sequence for T418K/Q442A after residue checks.

sequence_variant_note: Mutation signature T418K/Q442A matches the mutant residue at offset +0 and the reference residue at offset +26; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:A0A0G2QNK1 direct accession lookup on 2026-05-31; canonical wild-type sequence.


sequence_source: UniProtKB:A0A0G2QNK1 direct accession lookup on 2026-05-31; reconstructed variant sequence for T418K after residue checks.

sequence_variant_note: Mutation signature T418K matches the mutant residue at offset +0 and the reference residue at offset -3; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:A0A0G2QNK1 direct accession lookup on 2026-05-31; reconstructed variant sequence for T415D/Q442A after residue checks.


organism: Bacillus sp.

sequence_source: UniProtKB:B9W2C5 direct accession lookup on 2026-05-31; canonical wild-type sequence.


organism: Bacillus sp. HR03

sequence_source: UniProtKB B9W2C5 canonical sequence checked directly; reconstructed by applying E188F after confirming residue E at position 188.

sequence_variant_note: Mutation signature E188F matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB B9W2C5 canonical sequence checked directly; reconstructed by applying E188Y after confirming residue E at position 188.

sequence_variant_note: Mutation signature E188Y matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB REST A0A0G2QNK1 FASTA fetched 2026-05-31; mutation_signature S208G applied after residue check


sequence_source: UniProtKB REST A0A0G2QNK1 FASTA fetched 2026-05-31; mutation_signature S208G/F227A applied after residue checks

sequence_variant_note: Mutation signature S208G/F227A matches the mutant residue at offset +0 and the reference residue at offset -29; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB REST A0A0G2QNK1 FASTA fetched 2026-05-31; mutation_signature F227A applied after residue check

sequence_variant_note: Mutation signature F227A matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


substrate_name: Malachite green

substrate: Malachite green


sequence_source: UniProtKB REST A0A0G2QNK1 FASTA fetched 2026-05-31; mutation_signature Q442A applied after residue check

sequence_variant_note: Mutation signature Q442A matches the mutant residue at offset +0 and the reference residue at offset -15; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB REST A0A0G2QNK1 FASTA fetched 2026-05-31; mutation_signature T418K applied after residue check


sequence_source: UniProtKB REST A0A0G2QNK1 FASTA fetched 2026-05-31; mutation_signature T418K/Q442A applied after residue checks


sequence_source: UniProtKB B9W2C5 canonical sequence; E188R reconstructed during curation review

sequence_variant_note: Mutation signature E188R matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB B9W2C5 canonical sequence; E188A reconstructed during curation review

sequence_variant_note: Mutation signature E188A matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB B9W2C5 canonical sequence; E188I reconstructed during curation review

sequence_variant_note: Mutation signature E188I matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB B9W2C5 canonical sequence; E188L reconstructed during curation review

sequence_variant_note: Mutation signature E188L matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB B9W2C5 canonical sequence; E188V reconstructed during curation review

sequence_variant_note: Mutation signature E188V matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB B9W2C5 canonical sequence; E188K reconstructed during curation review

sequence_variant_note: Mutation signature E188K matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


organism: Bacillus sp. (in: Bacteria)


substrate_name: L-Dopa

sequence_source: UniProtKB:B9W2C5 direct REST lookup on 2026-05-31

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

substrate: L-Dopa


organism: Bacillus sp. SL-1

sequence_source: UniProtKB REST direct lookup A0A165W3F6; entry A0A165W3F6_9BACI, Bacillus sp. SL-1 laccase EC 1.10.3.2, 513 aa, reference PMID 27059481.


organism: Bacillus subtilis

sequence_source: UniProtKB REST direct lookup P07788 canonical CotA sequence; residue 502 is M and the selected source mutation M502L was reconstructed by replacing only residue 502 with leucine.

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


substrate_name: Potassium ferrocyanide

sequence_source: UniProtKB REST direct lookup P07788 canonical CotA sequence; residue 502 is M and the selected source mutation M502F was reconstructed by replacing only residue 502 with phenylalanine.

substrate: Potassium ferrocyanide


sequence_source: UniProtKB:P07788 canonical sequence with validated M502F substitution reconstructed on 2026-05-31


sequence_source: UniProtKB:P07788 reviewed FASTA/JSON direct lookup on 2026-05-31


sequence_source: UniProtKB:P07788 canonical sequence with validated I494A substitution reconstructed on 2026-05-31

sequence_variant_note: Mutation signature I494A matches the mutant residue at offset +0 and the reference residue at offset +14; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:P07788 canonical sequence with validated L386A substitution reconstructed on 2026-05-31


sequence_source: uniprot:P07788 + mutation:I494A

sequence_variant_note: Resolved canonical sequence from uniprot:P07788 + mutation:I494A and applied mutation signature I494A to reconstruct the assayed variant sequence.


substrate_name: Sudan Orange G

substrate: Sudan Orange G


sequence_source: uniprot:P07788 + mutation:G417L

sequence_variant_note: Resolved canonical sequence from uniprot:P07788 + mutation:G417L and applied mutation signature G417L to reconstruct the assayed variant sequence.


sequence_source: uniprot:P07788 + mutation:E498D

sequence_variant_note: Resolved canonical sequence from uniprot:P07788 + mutation:E498D and applied mutation signature E498D to reconstruct the assayed variant sequence.


sequence_source: uniprot:P07788 + mutation:E498L

sequence_variant_note: Resolved canonical sequence from uniprot:P07788 + mutation:E498L and applied mutation signature E498L to reconstruct the assayed variant sequence.


sequence_source: uniprot:P07788 + mutation:E498T

sequence_variant_note: Resolved canonical sequence from uniprot:P07788 + mutation:E498T and applied mutation signature E498T to reconstruct the assayed variant sequence.


sequence_source: UniProtKB:P07788 direct FASTA; reconstructed E498T by replacing canonical residue E at position 498 with T during review on 2026-05-31

sequence_variant_note: Mutation signature E498T matches the mutant residue at offset +0 and the reference residue at offset -2; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:P07788 direct FASTA; reconstructed E498L by replacing canonical residue E at position 498 with L during review on 2026-05-31

sequence_variant_note: Mutation signature E498L matches the mutant residue at offset +0 and the reference residue at offset -2; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:P07788 direct FASTA; reconstructed E498D by replacing canonical residue E at position 498 with D during review on 2026-05-31

sequence_variant_note: Mutation signature E498D matches the mutant residue at offset +0 and the reference residue at offset -2; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:P07788 direct FASTA; reconstructed C322A by replacing canonical residue C at position 322 with A during review on 2026-05-31


substrate_name: syringaldehyde

sequence_source: UniProtKB:P07788 direct FASTA canonical sequence checked during review on 2026-05-31

substrate: syringaldehyde


substrate_name: acetosyringone

substrate: acetosyringone


substrate_name: Methyl syringate

substrate: Methyl syringate


sequence_source: UniProtKB:P07788 canonical sequence direct lookup on 2026-05-31.


sequence_source: UniProtKB:P07788 canonical sequence direct lookup on 2026-05-31; T415A reconstructed after validating the reference residue in P07788.


sequence_source: UniProtKB:P07788 canonical sequence direct lookup on 2026-05-31; R416A reconstructed after validating the reference residue in P07788.

sequence_variant_note: Mutation signature R416A matches the mutant residue at offset +0 and the reference residue at offset +13; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB B9W2C5 canonical sequence retrieved by UniProt REST on 2026-05-31; row-specific T415G variant reconstructed after confirming canonical T415/T418 residues.

sequence_variant_note: Mutation signature T415G matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB B9W2C5 canonical sequence retrieved by UniProt REST on 2026-05-31; row-specific T418I variant reconstructed after confirming canonical T415/T418 residues.


sequence_source: UniProtKB B9W2C5 canonical sequence retrieved by UniProt REST on 2026-05-31; row-specific T415G/T418I variant reconstructed after confirming canonical T415/T418 residues.


sequence_source: UniProtKB B9W2C5 canonical sequence retrieved by UniProt REST on 2026-05-31; row-specific T415I variant reconstructed after confirming canonical T415/T418 residues.

sequence_variant_note: Mutation signature T415I matches the mutant residue at offset +0 and the reference residue at offset -9; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: Direct UniProt REST check of P07788 on 2026-05-31; canonical sequence was 513 aa and R146K was applied after residue R146 matched.


sequence_source: Direct UniProt REST check of P07788 on 2026-05-31; canonical sequence was 513 aa and P455S was applied after residue P455 matched.


sequence_source: Direct UniProt REST check of P07788 on 2026-05-31; canonical sequence was 513 aa and V403T plus P455S were applied after residues V403 and P455 matched.


sequence_source: Direct UniProt REST check of P07788 on 2026-05-31; canonical sequence was 513 aa and F207Y was applied after residue F207 matched.


sequence_source: Direct UniProt REST check of P07788 on 2026-05-31; canonical sequence was 513 aa and V403T was applied after residue V403 matched.

sequence_variant_note: Mutation signature V403T matches the mutant residue at offset +0 and the reference residue at offset -6; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: Direct UniProt REST check of A0A386JCD3 on 2026-05-31; canonical sequence was 513 aa and R155W, M157H, and A158E were applied after residues R155, M157, and A158 matched.


sequence_source: Direct UniProt REST check of A0A386JCD3 on 2026-05-31; canonical sequence was 513 aa and R155H plus A158E were applied after residues R155 and A158 matched.


enzyme_display_name: Laccase

column_label: Table 3: Kinetic parameters of CAR2, CAHH1, and mutant forms (Applied Microbiology and Biotechnology 2018, volume 102, pages 4039-4048); printed column heads Group | Clone | ABTS block Km (mM), kcat (s^-1), kcat/Km (s^-1 mM^-1) | DMP block Km (mM), Kcat (s^-1), kcat/Km (s^-1 mM^-1). Table 3, ABTS block, clone row CAR2 wild type; column Km (mM) = 0.21; column kcat (s^-1) = 8; column kcat/Km (s^-1 mM^-1) = 38. captured primary segment: CAR2 wild type | ABTS | Km 0.21 ± 0.02 mM | kcat 8 ± 0.03 s^-1 | kcat/Km 38 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 3 Km cell 0.21 mM; the printed unit is already the canonical millimolar unit, so no unit conversion applies and the value is 0.21 mM. kcat: printed Table 3 kcat cell 8 s^-1; the printed unit is already the canonical per-second unit, so no unit conversion applies and the value is 8.0 s^(-1). kcat/Km: printed Table 3 kcat/Km cell 38 s^-1 mM^-1; the printed unit is already the canonical per-second per-millimolar unit, so no unit conversion applies and the value is 38.0 s^(-1)*mM^(-1). Quotient disclosure for this cell group: 8 / 0.21 = 38.095238095238095 s^-1 mM^-1, which is the exported value, and the printed efficiency cell 38 is that quotient written to the integer place the table uses.

raw_value_unit_evidence: Table 3, ABTS block, clone row CAR2 wild type: Km = 0.21 mM; kcat = 8 s^-1; printed kcat/Km = 38 s^-1 mM^-1.

enzyme: Laccase


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:A0A386JCD3 and applied mutation signature R155H to reconstruct the assayed variant sequence.

column_label: Table 3: Kinetic parameters of CAR2, CAHH1, and mutant forms (Applied Microbiology and Biotechnology 2018, volume 102, pages 4039-4048); printed column heads Group | Clone | ABTS block Km (mM), kcat (s^-1), kcat/Km (s^-1 mM^-1) | DMP block Km (mM), Kcat (s^-1), kcat/Km (s^-1 mM^-1). Table 3, DMP block, clone row CAH2 R155H; column Km (mM) = 0.08; column kcat (s^-1) = 62; column kcat/Km (s^-1 mM^-1) = 786. captured primary segment: CAH2 R155H | DMP | Km 0.08 ± 0.009 mM | kcat 62 ± 0.8 s^-1 | kcat/Km 786 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 3 Km cell 0.08 mM; the printed unit is already the canonical millimolar unit, so no unit conversion applies and the value is 0.08 mM. kcat: printed Table 3 kcat cell 62 s^-1; the printed unit is already the canonical per-second unit, so no unit conversion applies and the value is 62.0 s^(-1). kcat/Km: printed Table 3 kcat/Km cell 786 s^-1 mM^-1; the printed unit is already the canonical per-second per-millimolar unit, so no unit conversion applies and the value is 786.0 s^(-1)*mM^(-1). Quotient disclosure for this cell group: 62 / 0.08 = 775.0 s^-1 mM^-1, against the printed efficiency cell 786.0 s^-1 mM^-1, so the printed cell is the paper's own fitted efficiency and not the quotient of its two rounded component cells.

raw_value_unit_evidence: Table 3, DMP block, clone row CAH2 R155H: Km = 0.08 mM; kcat = 62 s^-1; printed kcat/Km = 786 s^-1 mM^-1.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature T158A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature R155H to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature R155W/M157H/A158E to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature R155W/M157H to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:A0A386JCD3 and applied mutation signature R155W to reconstruct the assayed variant sequence.

column_label: Table 3: Kinetic parameters of CAR2, CAHH1, and mutant forms (Applied Microbiology and Biotechnology 2018, volume 102, pages 4039-4048); printed column heads Group | Clone | ABTS block Km (mM), kcat (s^-1), kcat/Km (s^-1 mM^-1) | DMP block Km (mM), Kcat (s^-1), kcat/Km (s^-1 mM^-1). Table 3, DMP block, clone row CAW R155W; column Km (mM) = 0.11; column kcat (s^-1) = 19; column kcat/Km (s^-1 mM^-1) = 172. captured primary segment: CAW R155W | DMP | Km 0.11 ± 0.03 mM | kcat 19 ± 0.3 s^-1 | kcat/Km 172 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 3 Km cell 0.11 mM; the printed unit is already the canonical millimolar unit, so no unit conversion applies and the value is 0.11 mM. kcat: printed Table 3 kcat cell 19 s^-1; the printed unit is already the canonical per-second unit, so no unit conversion applies and the value is 19.0 s^(-1). kcat/Km: printed Table 3 kcat/Km cell 172 s^-1 mM^-1; the printed unit is already the canonical per-second per-millimolar unit, so no unit conversion applies and the value is 172.0 s^(-1)*mM^(-1). Quotient disclosure for this cell group: 19 / 0.11 = 172.72727272727272 s^-1 mM^-1, against the printed efficiency cell 172.0 s^-1 mM^-1, so the printed cell is the paper's own fitted efficiency and not the quotient of its two rounded component cells.

raw_value_unit_evidence: Table 3, DMP block, clone row CAW R155W: Km = 0.11 mM; kcat = 19 s^-1; printed kcat/Km = 172 s^-1 mM^-1.

row_label: Table 3, DMP block, clone row CAW R155W


sequence_source: UniProtKB:A0A386JCD3 canonical sequenceVersion=1; row-specific variant reconstructed from R155H/A158E

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


sequence_source: UniProtKB:A0A1W6S310 canonical sequenceVersion=1 from direct UniProt REST lookup

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

column_label: Table 3: Kinetic parameters of CAR2, CAHH1, and mutant forms (Applied Microbiology and Biotechnology 2018, volume 102, pages 4039-4048); printed column heads Group | Clone | ABTS block Km (mM), kcat (s^-1), kcat/Km (s^-1 mM^-1) | DMP block Km (mM), Kcat (s^-1), kcat/Km (s^-1 mM^-1). Table 3, DMP block, clone row CAHH1 wild type; column Km (mM) = 0.07; column kcat (s^-1) = 79; column kcat/Km (s^-1 mM^-1) = 1134. captured primary segment: CAHH1 wild type | DMP | Km 0.07 ± 0.008 mM | kcat 79 ± 0.9 s^-1 | kcat/Km 1134 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 3 Km cell 0.07 mM; the printed unit is already the canonical millimolar unit, so no unit conversion applies and the value is 0.07 mM. kcat: printed Table 3 kcat cell 79 s^-1; the printed unit is already the canonical per-second unit, so no unit conversion applies and the value is 79.0 s^(-1). kcat/Km: printed Table 3 kcat/Km cell 1134 s^-1 mM^-1; the printed unit is already the canonical per-second per-millimolar unit, so no unit conversion applies and the value is 1134.0 s^(-1)*mM^(-1). Quotient disclosure for this cell group: 79 / 0.07 = 1128.5714285714284 s^-1 mM^-1, against the printed efficiency cell 1134.0 s^-1 mM^-1, so the printed cell is the paper's own fitted efficiency and not the quotient of its two rounded component cells.

raw_value_unit_evidence: Table 3, DMP block, clone row CAHH1 wild type: Km = 0.07 mM; kcat = 79 s^-1; printed kcat/Km = 1134 s^-1 mM^-1.


sequence_source: UniProtKB:A0A386JCD3 canonical sequenceVersion=1 from direct UniProt REST lookup

column_label: Table 3: Kinetic parameters of CAR2, CAHH1, and mutant forms (Applied Microbiology and Biotechnology 2018, volume 102, pages 4039-4048); printed column heads Group | Clone | ABTS block Km (mM), kcat (s^-1), kcat/Km (s^-1 mM^-1) | DMP block Km (mM), Kcat (s^-1), kcat/Km (s^-1 mM^-1). Table 3, DMP block, clone row CAR2 wild type; column Km (mM) = 0.14; column kcat (s^-1) = 21; column kcat/Km (s^-1 mM^-1) = 150. captured primary segment: CAR2 wild type | DMP | Km 0.14 ± 0.01 mM | kcat 21 ± 0.4 s^-1 | kcat/Km 150 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 3 Km cell 0.14 mM; the printed unit is already the canonical millimolar unit, so no unit conversion applies and the value is 0.14 mM. kcat: printed Table 3 kcat cell 21 s^-1; the printed unit is already the canonical per-second unit, so no unit conversion applies and the value is 21.0 s^(-1). kcat/Km: printed Table 3 kcat/Km cell 150 s^-1 mM^-1; the printed unit is already the canonical per-second per-millimolar unit, so no unit conversion applies and the value is 150.0 s^(-1)*mM^(-1). Quotient disclosure for this cell group: 21 / 0.14 = 149.99999999999997 s^-1 mM^-1, which is the printed efficiency cell itself.

raw_value_unit_evidence: Table 3, DMP block, clone row CAR2 wild type: Km = 0.14 mM; kcat = 21 s^-1; printed kcat/Km = 150 s^-1 mM^-1.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:A0A386JCD3 and applied mutation signature R155W/M157H to reconstruct the assayed variant sequence.

column_label: Table 3: Kinetic parameters of CAR2, CAHH1, and mutant forms (Applied Microbiology and Biotechnology 2018, volume 102, pages 4039-4048); printed column heads Group | Clone | ABTS block Km (mM), kcat (s^-1), kcat/Km (s^-1 mM^-1) | DMP block Km (mM), Kcat (s^-1), kcat/Km (s^-1 mM^-1). Table 3, DMP block, clone row CAWH R155W/M157H; column Km (mM) = 0.09; column kcat (s^-1) = 49; column kcat/Km (s^-1 mM^-1) = 545. captured primary segment: CAWH R155W/M157H | DMP | Km 0.09 ± 0.008 mM | kcat 49 ± 0.6 s^-1 | kcat/Km 545 s^-1 mM^-1

normalized_value_unit_evidence: Km: printed Table 3 Km cell 0.09 mM; the printed unit is already the canonical millimolar unit, so no unit conversion applies and the value is 0.09 mM. kcat: printed Table 3 kcat cell 49 s^-1; the printed unit is already the canonical per-second unit, so no unit conversion applies and the value is 49.0 s^(-1). kcat/Km: printed Table 3 kcat/Km cell 545 s^-1 mM^-1; the printed unit is already the canonical per-second per-millimolar unit, so no unit conversion applies and the value is 545.0 s^(-1)*mM^(-1). Quotient disclosure for this cell group: 49 / 0.09 = 544.4444444444445 s^-1 mM^-1, against the printed efficiency cell 545.0 s^-1 mM^-1, so the printed cell is the paper's own fitted efficiency and not the quotient of its two rounded component cells.

raw_value_unit_evidence: Table 3, DMP block, clone row CAWH R155W/M157H: Km = 0.09 mM; kcat = 49 s^-1; printed kcat/Km = 545 s^-1 mM^-1.


sequence_source: UniProtKB:A0A386JCD3 canonical sequenceVersion=1; row-specific variant reconstructed from R155W


sequence_source: UniProtKB:A0A348FKE9 canonical sequenceVersion=1 from direct UniProt REST lookup


sequence_variant_note: Resolved canonical sequence from UniProtKB REST B9W2C5; direct accession lookup; reconstructed_variant_sequence and applied mutation signature E188I to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB REST B9W2C5; direct accession lookup; reconstructed_variant_sequence and applied mutation signature E188R to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB REST B9W2C5; direct accession lookup; reconstructed_variant_sequence and applied mutation signature E188K to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB REST B9W2C5; direct accession lookup; reconstructed_variant_sequence and applied mutation signature E188L to reconstruct the assayed variant sequence.


sequence_source: UniProtKB REST B9W2C5; direct accession lookup; canonical_wild_type_sequence


organism: Bacillus sp. LS03

sequence_source: UniProtKB REST H8WGE3; direct accession lookup; canonical_wild_type_sequence


sequence_variant_note: Resolved canonical sequence from UniProtKB REST H8WGE3; direct accession lookup; reconstructed_variant_sequence at offset 0 and applied mutation signature T232P/Q367R to reconstruct the assayed variant sequence.


sequence_source: UniProtKB REST A0A165W3F6; direct accession lookup; canonical_wild_type_sequence


sequence_source: UniProtKB:P07788 canonical sequence; reconstructed R429K variant after residue check on 2026-05-31


sequence_source: UniProtKB:P07788 canonical sequence; reconstructed A478F variant after residue check on 2026-05-31


sequence_source: UniProtKB:P07788 canonical sequence; reconstructed T480A variant after residue check on 2026-05-31

sequence_variant_note: Mutation signature T480A matches the mutant residue at offset +0 and the reference residue at offset -14; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:P07788 canonical sequence; reconstructed T480F variant after residue check on 2026-05-31

sequence_variant_note: Mutation signature T480F matches the mutant residue at offset +0 and the reference residue at offset -14; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB:P07788 canonical sequence; reconstructed R476K variant after residue check on 2026-05-31

sequence_variant_note: Mutation signature R476K matches the mutant residue at offset +0 and the reference residue at offset +11; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


organism: Escherichia coli

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.


sequence_source: UniProtKB:P36649 reviewed FASTA checked 2026-05-31


sequence_source: UniProtKB:P36649 reviewed FASTA checked 2026-05-31; canonical sequence only, not a validated variant sequence

sequence_variant_note: Mutation signature G276K could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Mutation signature G276N could not be applied safely because the resolved sequence does not match the expected reference residue(s).


sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset +4, so it is treated as a source-provided variant sequence.


sequence_variant_note: Mutation signature G276Y could not be applied safely because the resolved sequence does not match the expected reference residue(s).


organism: Campylobacter jejuni

smiles: O=O


organism: Coprinopsis cinerea


organism: Coprinus comatus


substrate_name: syringaldazine

substrate: syringaldazine


substrate_name: guaiacol

substrate: guaiacol


organism: Coriolopsis trogii

sequence_source: UniProtKB:A0A140CWW4 canonical sequence, REST lookup on 2026-05-31


organism: Corynebacterium glutamicum

sequence_source: UniProtKB:Q8NLH5 canonical sequence, REST lookup on 2026-05-31


substrate_name: Syringaldazine

substrate: Syringaldazine


organism: Desulfovibrio sp. A2


organism: Fomitopsis pinicola


organism: Galerina sp. DSM 22662

sequence: GPSTDLVIGNKGFDGGIDSAILR

wild_type_sequence: GPSTDLVIGNKGFDGGIDSAILR

sequence_source: UniProtKB:P86351 fragment entry


organism: Ganoderma fornicatum


sequence_source: UniProtKB:B5G555 REST record


organism: Geobacillus sp. JS12


substrate_name: Guaiacol

substrate: Guaiacol


organism: Geobacillus sp. WSUCF1


organism: Halobacterium volcanii

sequence_source: UniProtKB:D4GPK6 REST record

column_label: Applied and Environmental Microbiology 76(3):733-743 (2010); TABLE 3. Kinetic parameters of H. volcanii LccA compared to those of selected laccase-like enzymes; LccA, H. volcanii block; sub-row 'Culture broth of H. volcanii SB01'; ABTS oxidation column group; column 'Km (uM)' = 700; column 'kcat (s^-1)' = 10.0; column 'kcat/Km (s^-1 uM^-1)' = 0.014.

normalized_value_unit_evidence: Printed kcat 10.0 s^-1 is already the catalog unit s^(-1); no conversion needed; catalog kcat = 10.0. Printed Km 700 uM / 1000 = 0.7 mM, matching the catalog km 0.7 mM exactly. The paper prints a kcat/Km cell for this table row, 0.014 s^-1 uM^-1 = 14.0 s^(-1)*mM^(-1), but that printed cell is NOT the catalog value: the catalog kcat_over_km 14.285714285714286 s^(-1)*mM^(-1) is the exact full-precision quotient of this row's own printed pair, 10.0 / 0.7. The printed cell is rounded to two significant figures, so the ratio is typed derived_same_measurement and the printed cell is documented in prose only.

raw_value_unit_evidence: TABLE 3, LccA H. volcanii block, sub-row 'Culture broth of H. volcanii SB01', ABTS oxidation column group: printed Km (uM) 700; printed kcat (s^-1) 10.0; printed kcat/Km (s^-1 uM^-1) 0.014. In the stored markup the Km column header carries U+03BC GREEK SMALL LETTER MU and every exponent minus is U+2212.

row_label: TABLE 3, LccA H. volcanii block, sub-row 'Culture broth of H. volcanii SB01', ABTS oxidation column group; printed Km (uM) 700, printed kcat (s^-1) 10.0, printed kcat/Km (s^-1 uM^-1) 0.014


organism: Klebsiella sp. 601

sequence_variant_note: Resolved canonical sequence from UniProtKB B0FMT2 current REST accession check on 2026-05-31; E106F reconstructed after confirming canonical residue 106 is E. and applied mutation signature E106F to reconstruct the assayed variant sequence.


sequence_source: UniProtKB B0FMT2 current REST accession check on 2026-05-31.


sequence_source: UniProtKB B0FMT2 current REST accession check on 2026-05-31; E106F reconstructed after confirming canonical residue 106 is E.


organism: Marinomonas mediterranea

sequence_source: UniProtKB Q9KJB8 current REST accession check on 2026-05-31.


organism: Meiothermus ruber

sequence_source: UniProtKB D3PLX1 current REST accession check on 2026-05-31.


sequence_source: UniProtKB:D3PLX1 direct REST lookup on 2026-05-31


organism: Mycothermus thermophilus


organism: Ochrobactrum sp. 531


organism: Paenibacillus glucanolyticus

sequence_source: UniProtKB:A0A165SC98 direct REST lookup on 2026-05-31


organism: Bacillus subtilis


organism: Caldalkalibacillus thermarum

sequence_source: UniProtKB:F5L3U7 direct accession lookup


organism: Campylobacter jejuni

sequence_source: UniProtKB:A0A0H3PBA4 direct accession lookup


organism: Pseudomonas putida

sequence_source: UniProtKB REST FASTA Q88C03; canonical wild-type sequence checked 2026-05-31

assay_conditions_summary: pH 4; 293.15 K; buffer to the 2

column_label: Table 3 (Steady-state kinetic constants for Pp-CopA and Pf-CopA), substrate block 2,6-Dimethoxyphenol (DMP), enzyme row Pp-CopA; column KM [uM] = 1459; column Vmax [U/mg prot.] = 3.4; column kcat [s-1] = 3.97; column Specificity constant kcat/KM [M^-1 s^-1] = 2700, printed with a standard deviation of 100.

normalized_value_unit_evidence: kcat: printed Table 3 cell 3.97 under the head kcat [s-1]; the printed unit is already canonical and no unit conversion applies, so kcat = 3.97 s^(-1). Km: printed Table 3 cell 1459 under the head KM [uM]; 1 uM is 0.001 mM, so 1459 / 1000 = 1.459, and Km = 1.459 mM. kcat/Km: printed Table 3 cell 2700 under the head kcat/KM [M^-1 s^-1]; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 2700 / 1000 = 2.7, and kcat/Km = 2.7 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 3, 2,6-Dimethoxyphenol (DMP) block, Pp-CopA row: KM [uM] cell = 1459; Vmax [U/mg prot.] cell = 3.4; kcat [s-1] cell = 3.97; specificity constant kcat/KM [M^-1 s^-1] cell = 2700, printed with a standard deviation of 100.

table_label: Table 3: Steady-state kinetic constants for Pp-CopA and Pf-CopA (Archives of Biochemistry and Biophysics 2018, author accepted manuscript)


assay_conditions_summary: pH 4; 293.15 K; buffer to the ABTS block

column_label: Table 3 (Steady-state kinetic constants for Pp-CopA and Pf-CopA), substrate block ABTS, enzyme row Pp-CopA; column KM [uM] = 488; column Vmax [U/mg prot.] = 2.0; column kcat [s-1] = 2.40; column Specificity constant kcat/KM [M^-1 s^-1] = 4900, printed with a standard deviation of 90.

normalized_value_unit_evidence: kcat: printed Table 3 cell 2.40 under the head kcat [s-1]; the printed unit is already canonical and no unit conversion applies, so kcat = 2.4 s^(-1). Km: printed Table 3 cell 488 under the head KM [uM]; 1 uM is 0.001 mM, so 488 / 1000 = 0.488, and Km = 0.488 mM. kcat/Km: printed Table 3 cell 4900 under the head kcat/KM [M^-1 s^-1]; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 4900 / 1000 = 4.9, and kcat/Km = 4.9 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 3, ABTS block, Pp-CopA row: KM [uM] cell = 488; Vmax [U/mg prot.] cell = 2.0; kcat [s-1] cell = 2.40; specificity constant kcat/KM [M^-1 s^-1] cell = 4900, printed with a standard deviation of 90.

row_label: Table 3, ABTS block, Pp-CopA row


organism: Pseudomonas sp.

sequence_source: UniProtKB REST FASTA A0A0K1P839; canonical wild-type sequence checked 2026-05-31


organism: Pyrobaculum aerophilum

sequence_source: UniProtKB REST FASTA Q8ZWA8; canonical wild-type sequence checked 2026-05-31


sequence_source: UniProtKB REST FASTA Q8ZWA8; M470F reconstructed after confirming residue 470 was M on 2026-05-31

sequence_variant_note: Mutation signature M470F matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB REST FASTA Q8ZWA8; M470L reconstructed after confirming residue 470 was M on 2026-05-31

sequence_variant_note: Mutation signature M470L matches the mutant residue at offset +0 and the reference residue at offset +1; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.


sequence_source: UniProtKB live accession A0A165SC98, checked by REST/FASTA on 2026-05-31.

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

proof_lines: OUP PDF Table 1: ABTS row at pH 7.0 and 25 deg C reports Km 38.8 +/- 11.1 micromol l^-1, Kcat 1400 s^-1, and Kcat/Km 36.1.


proof_lines: OUP PDF Table 1: catechol row at pH 7.0 and 25 deg C reports Km 11669 +/- 2015 micromol l^-1, Kcat 8805 s^-1, and Kcat/Km 0.75.


organism: Pleurotus eryngii

sequence_variant_note: Resolved canonical sequence from UniProtKB live accession B0JDP9 canonical sequence with in-review K532E reconstruction; residue 532 was checked as K on 2026-05-31. and applied mutation signature K532E to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB live accession B0JDP9 canonical sequence with in-review P530A reconstruction; residue 530 was checked as P on 2026-05-31. and applied mutation signature P530A to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from UniProtKB live accession B0JDP9 canonical sequence with in-review K532A reconstruction; residue 532 was checked as K on 2026-05-31. and applied mutation signature K532A to reconstruct the assayed variant sequence.


organism: Pleurotus ostreatus

sequence_source: UniProtKB live accession O60199, checked by REST/FASTA on 2026-05-31.


sequence_source: UniProtKB reviewed accession Q12739, checked by REST/FASTA on 2026-05-31.


sequence_source: UniProtKB:D4GPK6 direct lookup on 2026-05-31


organism: Haloferax volcanii

substrate_name: bilirubin

column_label: Applied and Environmental Microbiology 76(3):733-743 (2010); Results, bilirubin specificity sentence; substrate bilirubin; printed 'kcat, 29 s^-1'; printed 'Km, 236 uM'; printed 'kcat/Km value of 0.12 s^-1 uM^-1'.

normalized_value_unit_evidence: Printed kcat 29 s^-1 is already the catalog unit s^(-1); no conversion needed; catalog kcat = 29.0. Printed Km 236 uM / 1000 = 0.236 mM, matching the catalog km 0.236 mM exactly. The paper prints a ratio for this measurement, kcat/Km value of 0.12 s^-1 uM^-1 = 120.0 s^(-1)*mM^(-1), but that printed value is NOT the catalog value: the catalog kcat_over_km 122.88135593220339 s^(-1)*mM^(-1) is the exact full-precision quotient of this measurement's own printed pair, 29.0 / 0.236. The printed ratio is rounded to two significant figures, so the ratio is typed derived_same_measurement and the printed value is documented in prose only.

raw_value_unit_evidence: Results, bilirubin specificity sentence: printed kcat, 29 s^-1; printed Km, 236 uM; printed kcat/Km value of 0.12 s^-1 uM^-1. In the stored asset the micro sign is U+03BC and every exponent minus is U+2212.

row_label: Results bilirubin specificity sentence; printed kcat, 29 s^-1, printed Km, 236 uM, printed kcat/Km value of 0.12 s^-1 uM^-1

table_label: Results, bilirubin specificity sentence (no table; the paper prints this pair in prose)

substrate: bilirubin


column_label: Applied and Environmental Microbiology 76(3):733-743 (2010); Abstract, apparent kinetic parameters sentence; ABTS position; printed Km 670 uM; printed kcat 10 s^-1. The same measurement is printed one digit longer in TABLE 3. Kinetic parameters of H. volcanii LccA compared to those of selected laccase-like enzymes, sub-row 'Culture broth of H. volcanii US02', ABTS oxidation column group: Km (uM) 671, kcat (s^-1) 9.9, kcat/Km (s^-1 uM^-1) 0.015.

normalized_value_unit_evidence: Printed kcat 10 s^-1 is already the catalog unit s^(-1); no conversion needed; catalog kcat = 10.0. Printed Km 670 uM / 1000 = 0.67 mM, matching the catalog km 0.67 mM exactly. The Abstract sentence prints no ratio for this pair. The TABLE 3 sub-row that prints the same US02 ABTS measurement to one more digit (Km 671 uM, kcat 9.9 s^-1) carries the ratio cell 0.015 s^-1 uM^-1 = 15.0 s^(-1)*mM^(-1), which is not the catalog value either: the catalog kcat_over_km 14.925373134328357 s^(-1)*mM^(-1) is the exact full-precision quotient of the Abstract pair, 10.0 / 0.67. The ratio is therefore typed derived_same_measurement and the printed table cell is documented in prose only.

raw_value_unit_evidence: Abstract, apparent kinetic parameters sentence: printed Km 670 uM and printed kcat 10 s^-1 for ABTS. In the stored asset the micro sign is U+03BC and the exponent minus is U+2212.

row_label: Abstract apparent kinetic parameters sentence, ABTS position; printed Km 670 uM and printed kcat 10 s^-1

table_label: Abstract, apparent kinetic parameters sentence (no table; the paper prints this pair in prose)


organism: Klebsiella pneumoniae


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.


organism: Rheinheimera sp.


substrate_name: sinapate

substrate: sinapate


organism: Rhizobium meliloti

sequence_source: UniProtKB:A0A0D4CYE3 direct REST lookup on 2026-05-31


sequence_source: UniProtKB REST direct lookup for O60199 on 2026-05-31


sequence_source: UniProtKB REST direct lookup for Q12739 on 2026-05-31


organism: Pseudomonas fluorescens

sequence_source: UniProtKB REST direct lookup for Q4KCN4 on 2026-05-31

column_label: Table 3 (Steady-state kinetic constants for Pp-CopA and Pf-CopA), substrate block 2,6-Dimethoxyphenol (DMP), enzyme row Pf-CopA; column KM [uM] = 822; column Vmax [U/mg prot.] = 1.4; column kcat [s-1] = 1.42; column Specificity constant kcat/KM [M^-1 s^-1] = 1700, printed with a standard deviation of 20.

normalized_value_unit_evidence: kcat: printed Table 3 cell 1.42 under the head kcat [s-1]; the printed unit is already canonical and no unit conversion applies, so kcat = 1.42 s^(-1). Km: printed Table 3 cell 822 under the head KM [uM]; 1 uM is 0.001 mM, so 822 / 1000 = 0.822, and Km = 0.822 mM. kcat/Km: printed Table 3 cell 1700 under the head kcat/KM [M^-1 s^-1]; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 1700 / 1000 = 1.7, and kcat/Km = 1.7 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 3, 2,6-Dimethoxyphenol (DMP) block, Pf-CopA row: KM [uM] cell = 822; Vmax [U/mg prot.] cell = 1.4; kcat [s-1] cell = 1.42; specificity constant kcat/KM [M^-1 s^-1] cell = 1700, printed with a standard deviation of 20.


column_label: Table 3 (Steady-state kinetic constants for Pp-CopA and Pf-CopA), substrate block ABTS, enzyme row Pf-CopA; column KM [uM] = 214; column Vmax [U/mg prot.] = 2.2; column kcat [s-1] = 2.20; column Specificity constant kcat/KM [M^-1 s^-1] = 10300, printed with a standard deviation of 160.

normalized_value_unit_evidence: kcat: printed Table 3 cell 2.20 under the head kcat [s-1]; the printed unit is already canonical and no unit conversion applies, so kcat = 2.2 s^(-1). Km: printed Table 3 cell 214 under the head KM [uM]; 1 uM is 0.001 mM, so 214 / 1000 = 0.214, and Km = 0.214 mM. kcat/Km: printed Table 3 cell 10300 under the head kcat/KM [M^-1 s^-1]; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 10300 / 1000 = 10.3, and kcat/Km = 10.3 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 3, ABTS block, Pf-CopA row: KM [uM] cell = 214; Vmax [U/mg prot.] cell = 2.2; kcat [s-1] cell = 2.20; specificity constant kcat/KM [M^-1 s^-1] cell = 10300, printed with a standard deviation of 160.

row_label: Table 3, ABTS block, Pf-CopA row


assay_conditions_summary: pH 7; 293.15 K; buffer pH 7

column_label: Table 3 (Steady-state kinetic constants for Pp-CopA and Pf-CopA), substrate block Syringaldazine (SGZ), enzyme row Pf-CopA; column KM [uM] = 51; column Vmax [U/mg prot.] = 1.2; column kcat [s-1] = 1.22; column Specificity constant kcat/KM [M^-1 s^-1] = 23800, printed with a standard deviation of 500.

normalized_value_unit_evidence: kcat: printed Table 3 cell 1.22 under the head kcat [s-1]; the printed unit is already canonical and no unit conversion applies, so kcat = 1.22 s^(-1). Km: printed Table 3 cell 51 under the head KM [uM]; 1 uM is 0.001 mM, so 51 / 1000 = 0.051, and Km = 0.051 mM. kcat/Km: printed Table 3 cell 23800 under the head kcat/KM [M^-1 s^-1]; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 23800 / 1000 = 23.8, and kcat/Km = 23.8 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 3, Syringaldazine (SGZ) block, Pf-CopA row: KM [uM] cell = 51; Vmax [U/mg prot.] cell = 1.2; kcat [s-1] cell = 1.22; specificity constant kcat/KM [M^-1 s^-1] cell = 23800, printed with a standard deviation of 500.


sequence_source: UniProtKB REST direct lookup for Q88C03 on 2026-05-31

column_label: Table 3 (Steady-state kinetic constants for Pp-CopA and Pf-CopA), substrate block Syringaldazine (SGZ), enzyme row Pp-CopA; column KM [uM] = 26; column Vmax [U/mg prot.] = 1.1; column kcat [s-1] = 1.28; column Specificity constant kcat/KM [M^-1 s^-1] = 49000, printed with a standard deviation of 2000.

normalized_value_unit_evidence: kcat: printed Table 3 cell 1.28 under the head kcat [s-1]; the printed unit is already canonical and no unit conversion applies, so kcat = 1.28 s^(-1). Km: printed Table 3 cell 26 under the head KM [uM]; 1 uM is 0.001 mM, so 26 / 1000 = 0.026, and Km = 0.026 mM. kcat/Km: printed Table 3 cell 49000 under the head kcat/KM [M^-1 s^-1]; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 49000 / 1000 = 49.0, and kcat/Km = 49.0 s^(-1)*mM^(-1).

raw_value_unit_evidence: Table 3, Syringaldazine (SGZ) block, Pp-CopA row: KM [uM] cell = 26; Vmax [U/mg prot.] cell = 1.1; kcat [s-1] cell = 1.28; specificity constant kcat/KM [M^-1 s^-1] cell = 49000, printed with a standard deviation of 2000.


sequence_source: UniProtKB exact accession A0A0D4CYE3 REST lookup; live unreviewed entry, organism Rhizobium meliloti, EC 1.10.3.2.


organism: Rhodococcus erythropolis


organism: Staphylococcus haemolyticus

sequence_source: UniProtKB exact accession L7SV51 REST lookup; live unreviewed entry, organism Staphylococcus haemolyticus, multicopper oxidase with no explicit UniProt EC.


organism: Stenotrophomonas maltophilia

sequence_source: UniProtKB exact accession G8XUN7 REST lookup; live unreviewed entry, organism Stenotrophomonas maltophilia, EC 1.10.3.2.


organism: Streptomyces californicus


organism: Streptomyces coelicolor


sequence_source: UniProt:Q9XAL8 live accession check on 2026-05-31; unreviewed TrEMBL copper oxidase from Streptomyces coelicolor strain ATCC BAA-471 / A3(2) / M145.


substrate_name: Morin

sequence_source: UniProtKB REST/FASTA Q9XAL8 canonical sequence, row-specific S292A substitution reconstructed after confirming canonical residue S at position 292.

sequence_variant_note: Mutation signature S292A matches the mutant residue at offset +0 and the reference residue at offset +2; variant reconstruction was not applied because multiple plausible residue-numbering offsets are present.

substrate: Morin


sequence_source: UniProtKB REST/FASTA Q9XAL8 canonical sequence.


sequence_source: UniProtKB REST/FASTA Q9XAL8 canonical sequence, row-specific V290A substitution reconstructed after confirming canonical residue V at position 290.

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.


sequence_source: UniProtKB REST/FASTA Q9XAL8 canonical sequence, row-specific M298F substitution reconstructed after confirming canonical residue M at position 298.


sequence_source: UniProtKB REST/FASTA Q9XAL8 canonical sequence, row-specific V290N substitution reconstructed after confirming canonical residue V at position 290.


sequence_source: UniProtKB REST accession Q9XAL8 checked 2026-05-31


organism: Streptomyces cyaneus

sequence_source: UniProtKB REST accession F6L7B5 checked 2026-05-31