substrate_name: VR

smiles: CCN(CC)CCSP(=O)(C)OCC(C)C

proof_lines: mutant enzyme C59L/C227A/D203N, at pH 8.0 and 37°C

substrate: VR


enzyme_display_name: aryldialkylphosphatase

organism: Brevundimonas diminuta

substrate_name: cyclosarin

source_db: brenda

verification_status: unverified

kcat_display: Not reported

km_display: Not reported

ki_display: Not reported

kcat_over_km_display: Not reported

mutation_signature: Unknown

source_license: CC BY 4.0

sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

enzyme: aryldialkylphosphatase

substrate: cyclosarin

status: unverified

source: brenda


sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: mutant enzyme C59V/C227V/S267L, at pH 8.0 and 37°C


proof_lines: mutant enzyme C59L/C227A/S267M, at pH 8.0 and 37°C


proof_lines: mutant enzyme C59V/C227V/S267M, at pH 8.0 and 37°C


proof_lines: mutant enzyme C59V/C227V, at pH 8.0 and 37°C


proof_lines: mutant enzyme I106A/C59V/C227V/S267M, at pH 8.0 and 37°C


substrate_name: VX

smiles: CCOP(=O)(C)SCCN(C(C)C)C(C)C

substrate: VX


substrate_name: sarin

smiles: CC(C)OP(=O)(C)F

substrate: sarin


organism: Saccharolobus islandicus

substrate_name: paraoxon

km_unit: mM

mutation_type: substitution

proof_lines: 25°C, pH not specified in the publication, mutant enzyme Y34Q

substrate: paraoxon


proof_lines: 25°C, pH not specified in the publication, mutant enzyme E14K


proof_lines: 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q


substrate_name: methyl paraoxon

substrate: methyl paraoxon


proof_lines: 70°C, pH not specified in the publication

proof_lines: 25°C, 0.01% SDS, pH not specified in the publication

proof_lines: 25°C, 0.1% SDS, pH not specified in the publication


proof_lines: 25°C, pH not specified in the publication, wild-type enzyme


substrate_name: malathion

smiles: CCOC(=O)CC(C(=O)OCC)SP(=S)(OC)OC

substrate: malathion


substrate_name: methyl parathion

substrate: methyl parathion


enzyme_display_name: protein-tyrosine-phosphatase

organism: Homo sapiens

substrate_name: DADEpYLIPQQG

mutation_signature: CCPGCC-insertion mutant PTPH1-816

mutation_type: insertion

proof_lines: CCPGCC-insertion mutant PTPH1-816, in the absence of fluorescein arsenical hairpin binder, in 50 mM 3,3-dimethylglutarate at pH 7.0, 1 mM EDTA, and 50 mM NaCl, at 22°C

proof_lines: CCPGCC-insertion mutant PTPH1-816, in the presence of fluorescein arsenical hairpin binder, in 50 mM 3,3-dimethylglutarate at pH 7.0, 1 mM EDTA, and 50 mM NaCl, at 22°C

enzyme: protein-tyrosine-phosphatase

substrate: DADEpYLIPQQG


mutation_signature: CCPGCC-insertion mutant PTPalpha-406

proof_lines: CCPGCC-insertion mutant PTPalpha-406, in the absence of fluorescein arsenical hairpin binder, in 50 mM 3,3-dimethylglutarate at pH 7.0, 1 mM EDTA, and 50 mM NaCl, at 22°C

proof_lines: CCPGCC-insertion mutant PTPalpha-406, in the presence of fluorescein arsenical hairpin binder, in 50 mM 3,3-dimethylglutarate at pH 7.0, 1 mM EDTA, and 50 mM NaCl, at 22°C


substrate_name: Unknown

mutation_signature: CCPGCC-insertion mutant TCPTP-187

ki_unit: mM

substrate: Unknown


mutation_signature: CCPGCC-insertion mutant PTPHalpha-406


mutation_signature: CCPGCC-insertion mutant PTP1B-186


mutation_signature: CCPGCC-insertion mutant PTP-PEST-204


mutation_signature: CCPGCC-insertion mutant HePTPH-211


mutation_signature: CCPGCC-insertion mutant FAP-1-2364


proof_lines: mutant enzyme C59L/C227A/D203N, at pH 8.0 and 37°C


proof_lines: mutant enzyme C59V/C227V/S267L, at pH 8.0 and 37°C


enzyme_display_name: phosphodiesterase I

organism: Arabidopsis thaliana

enzyme: phosphodiesterase I


substrate_name: demeton-S

smiles: CCOP(=O)(OCC)SCCSCC

assay_conditions_summary: pH 8; buffer tripart

proof_lines: mutant enzyme H257L, in tripart buffer (pH 8.0)

substrate: demeton-S


proof_lines: wild type enzyme, in tripart buffer (pH 8.0)


proof_lines: mutant enzyme H254R, in tripart buffer (pH 8.0)


proof_lines: mutant enzyme H254R/H257F, in tripart buffer (pH 8.0)


proof_lines: mutant enzyme H254R/H257L, in tripart buffer (pH 8.0)


assay_conditions_summary: pH 9; 298.15 K; buffer 20 mM CHES

proof_lines: mutant enzyme H254R/H257F, in 20 mM CHES buffer (pH 9.0) at 25°C


proof_lines: mutant enzyme H254R/H257L, in 20 mM CHES buffer (pH 9.0) at 25°C


proof_lines: mutant enzyme H257L, in 20 mM CHES buffer (pH 9.0) at 25°C


proof_lines: Co2+-PTE mutant H123I, at pH 8.5 and 30°C


assay_conditions_summary: pH 10.5; 298.15 K; buffer 50 mM borate

proof_lines: pH 10.5, 25°C, 3-fluorotyrosine-containing recombinant His6-tagged OPH


proof_lines: Co2+-PTE mutant H123A, at pH 8.5 and 30°C


proof_lines: Co2+-PTE mutant H123N, at pH 8.5 and 30°C


proof_lines: Co2+-PTE mutant H123N, at pH 8.5 and 30°C, with paraoxon as substrate


proof_lines: Co2+-PTE mutant H123A, at pH 8.5 and 30°C, with paraoxon as substrate


proof_lines: Co2+-PTE mutant H123I, at pH 8.5 and 30°C, with paraoxon as substrate


enzyme_display_name: glycerophosphodiester phosphodiesterase

organism: Escherichia coli

substrate_name: glycerophosphoethanolamine

smiles: C(COP(=O)(O)OCC(CO)O)N

enzyme: glycerophosphodiester phosphodiesterase

substrate: glycerophosphoethanolamine


substrate_name: glycerophosphoinositol

substrate: glycerophosphoinositol


substrate_name: glycerophosphocholine

substrate: glycerophosphocholine


substrate_name: glycerophosphoglycerol

smiles: C(C(COP(=O)(O)OCC(CO)O)O)O

substrate: glycerophosphoglycerol


substrate_name: glycerophosphoserine

smiles: C(C(COP(=O)(O)OCC(C(=O)O)N)O)O

substrate: glycerophosphoserine


organism: Bos taurus

substrate_name: NAD+

assay_conditions_summary: cofactor NAD+

substrate: NAD+


substrate_name: p-nitrophenylthymidine 5'-phosphate

proof_lines:
milk fat globule membrane
cytoplasmic membrane of mammary gland

substrate: p-nitrophenylthymidine 5'-phosphate


substrate_name: ATP

assay_conditions_summary: cofactor ATP

substrate: ATP


substrate_name: bis(p-nitrophenyl) phosphate

substrate: bis(p-nitrophenyl) phosphate


substrate_name: p-Nitrophenyl phenylphosphonate

proof_lines:
cytoplasmic membrane of mammary gland
milk fat globule membrane

substrate: p-Nitrophenyl phenylphosphonate


substrate_name: p-nitrophenyl phosphate

substrate: p-nitrophenyl phosphate


proof_lines: mutant enzyme BHR-75, at pH 8.0 and 30°C

proof_lines: mutant enzyme BHR-73, at pH 8.0 and 30°C

proof_lines: mutant enzyme BHR-73-MNW, at pH 8.0 and 30°C


proof_lines: recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C

proof_lines: pH 9.0, 30°C, recombinant wild-type enzyme


proof_lines: mutant enzyme BHR-72, at pH 8.0 and 30°C

proof_lines: mutant enzyme BHR-33, at pH 8.0 and 30°C

proof_lines: mutant enzyme BHR-54, at pH 8.0 and 30°C


proof_lines:
wild type enzyme, at pH 8.0 and 30°C
wild type enzyme, at pH 8.0 and 37°C


substrate_name: X12 junction in single-stranded DNA

proof_lines: like nX12, without the nick at the cross-over point

proof_lines: in 25 mM Tris-HCl, pH 8.0, 1 mM MgCl2, 0.2 mM dithiothreitol, at 37°C

proof_lines: the 3'flap substrate contains 50 bp of duplex DNA, a central nick, and 25 bases of single-stranded DNA

substrate: X12 junction in single-stranded DNA


enzyme_display_name: alpha-amylase

organism: Struthio camelus

substrate_name: maltose

protein_accession_database: UniProt

sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.

data_origin: experimental

enzyme: alpha-amylase

substrate: maltose


substrate_name: maltotriose

substrate: maltotriose


substrate_name: maltohexaose

substrate: maltohexaose


substrate_name: maltoheptaose

substrate: maltoheptaose


substrate_name: maltopentaose

substrate: maltopentaose


enzyme_display_name: UniProt B5ARZ9

organism: Thermotoga neapolitana

substrate_name: 4-nitrophenyl alpha-D-maltohexaoside

source_db: skid

source_license: CC BY-NC-ND 4.0

enzyme: UniProt B5ARZ9

substrate: 4-nitrophenyl alpha-D-maltohexaoside

source: skid


organism: Escherichia coli K-12

substrate_name: glycerophosphoethanolamine

kcat_over_km_origin: calculated

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

substrate: glycerophosphoethanolamine


substrate_name: glycerophosphoinositol

substrate: glycerophosphoinositol


substrate_name: glycerophosphocholine

substrate: glycerophosphocholine


substrate_name: glycerophosphoglycerol

substrate: glycerophosphoglycerol


enzyme_display_name: type II site-specific deoxyribonuclease

organism: Brevibacillus brevis

substrate_name: DNA

proof_lines:
37°C, R1+-R2- mutant
37°C, R1-R2+ mutant

enzyme: type II site-specific deoxyribonuclease

substrate: DNA


enzyme_display_name: deoxyribonuclease V

organism: Mus musculus

substrate_name: ACUGGACA[rI][rI]U[rI]CUCCGAGG

enzyme: deoxyribonuclease V

substrate: ACUGGACA[rI][rI]U[rI]CUCCGAGG


enzyme_display_name: T4 deoxyribonuclease IV

organism: Tequatrovirus T4

enzyme: T4 deoxyribonuclease IV


organism: Thermococcus kodakarensis

substrate_name: 30-mer DNA/RNA containing inosine hybrid


enzyme_display_name: Crossover junction endodeoxyribonuclease Hje

organism: Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

substrate_name: 4-way junction DNA at 5 mM MgCl2

source_db: uniprot

proof_lines: kcat is 4.8 min(-1) and 10 min(-1) at 5 mM and 15 mM MgCl2 respectively.

substrate: 4-way junction DNA at 5 mM MgCl2

source: uniprot


organism: Schizosaccharomyces pombe

substrate_name: DNA junction 1 unconstrained

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_mutation_ranked_match and applied mutation signature E226N with inferred residue-numbering offset -4 to reconstruct the assayed variant sequence.

substrate: DNA junction 1 unconstrained


organism: Bacillus amyloliquefaciens

substrate_name: NTP14 DNA

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

proof_lines:
BamHI, 37°C
Hpa, 25°C

substrate: NTP14 DNA


organism: Bergeriella denitrificans

substrate_name: linear mNeonGreen DNA

proof_lines: 37°C, pH not specified in the publication

substrate: linear mNeonGreen DNA


substrate_name: 49-mer oligonucleotide

proof_lines: mutant K121N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer

substrate: 49-mer oligonucleotide


proof_lines: mutant R117N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer


proof_lines: mutant Q15R enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer


proof_lines: mutant K86N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer


proof_lines: wild type enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer


proof_lines: mutant Q15R and R117N enzymes, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer


substrate_name: 4-nitrophenyl phosphate

substrate: 4-nitrophenyl phosphate


enzyme_display_name: exodeoxyribonuclease III

organism: Mycobacterium tuberculosis

substrate_name: N1 duplex DNA

enzyme: exodeoxyribonuclease III

substrate: N1 duplex DNA


proof_lines: pH 7.8, 37°C, recombinant mutant Y234S/W235S


proof_lines: pH 7.8, 37°C, recombinant wild-type enzyme


substrate_name: p-nitrophenyl phosphate

substrate: p-nitrophenyl phosphate


proof_lines:
wild type enzyme, at pH 7.0 and 25°C
wild-type PTPalpha, 25°C, pH 7.0
pH 7.0, 22°C, wild-type enzyme


proof_lines: mutant enzyme S35E/T36E, at pH 7.0 and 25°C


enzyme_display_name: Terminase, large subunit

organism: Escherichia phage lambda

substrate_name: ATP for the high affinity ATPase

proof_lines: The high affinity ATPase activity corresponds to the packaging ATPase site at the N-terminus.

enzyme: Terminase, large subunit

substrate: ATP for the high affinity ATPase


organism: Rattus norvegicus

substrate_name: 1-naphthyl phosphate

substrate: 1-naphthyl phosphate


substrate_name: 4-methylumbelliferyl phosphate

substrate: 4-methylumbelliferyl phosphate


substrate_name: Phenyl phosphate

substrate: Phenyl phosphate


substrate_name: phosphotyrosine

substrate: phosphotyrosine


enzyme_display_name: feruloyl esterase

organism: uncultured microorganism

substrate_name: methyl sinapate

proof_lines:
recombinant enzyme, pH 8.0, 37°C
pH 5.0, 30°C

enzyme: feruloyl esterase

substrate: methyl sinapate


substrate_name: methyl vanillate

substrate: methyl vanillate


substrate_name: methyl caffeate

substrate: methyl caffeate


substrate_name: ethyl ferulate

substrate: ethyl ferulate


organism: Aspergillus oryzae


substrate_name: methyl 4-coumarate

substrate: methyl 4-coumarate


substrate_name: methyl ferulate

substrate: methyl ferulate


organism: Brevundimonas diminuta

smiles: CC(C(C)(C)C)OP(=O)(C)F


substrate_name: diisopropylfluorophosphate

smiles: CC(C)OP(=O)(OC(C)C)F

substrate: diisopropylfluorophosphate


substrate_name: isopropylmethylphosphonofluoridate

proof_lines: Co2+-PTE, at pH 8.5 and 30°C, with paraoxon as substrate

proof_lines: Zn2+-PTE, at pH 8.5 and 30°C, with paraoxon as substrate

substrate: isopropylmethylphosphonofluoridate


enzyme_display_name: ribonuclease III

organism: Drosophila melanogaster

substrate_name: ATP (in the presence of 515 bp dsRNA)

proof_lines: kcat is 0.03 min(-1) for dsRNA consumption (PubMed:21419681).

enzyme: ribonuclease III

substrate: ATP (in the presence of 515 bp dsRNA)


substrate_name: paraoxon

proof_lines: mutant enzyme H254R, in 20 mM CHES buffer (pH 9.0) at 25°C

substrate: paraoxon


substrate_name: diethyl 4-methylbenzylphosphonate

proof_lines: mutant H254R, pH 9.0, 25°C, versus paraoxon

substrate: diethyl 4-methylbenzylphosphonate


proof_lines: wild-type enzyme, pH 9.0, 25°C, versus paraoxon


enzyme_display_name: acyl-CoA hydrolase

organism: Escherichia coli

substrate_name: myristoyl-CoA

assay_conditions_summary: cofactor CoA

enzyme: acyl-CoA hydrolase

substrate: myristoyl-CoA


enzyme_display_name: cutinase

organism: Fusarium oxysporum

substrate_name: 4-nitrophenyl laurate

enzyme: cutinase

substrate: 4-nitrophenyl laurate


substrate_name: 4-nitrophenyl butanoate

substrate: 4-nitrophenyl butanoate


substrate_name: 4-nitrophenyl acetate

substrate: 4-nitrophenyl acetate


substrate_name: n-propyl ferulate

substrate: n-propyl ferulate


substrate_name: n-butyl ferulate

substrate: n-butyl ferulate


enzyme_display_name: fructose-2,6-bisphosphate 2-phosphatase

organism: Homo sapiens

substrate_name: beta-D-fructose 2,6-bisphosphate

enzyme: fructose-2,6-bisphosphate 2-phosphatase

substrate: beta-D-fructose 2,6-bisphosphate


organism: Fusarium proliferatum


substrate_name: methyl p-coumarate

substrate: methyl p-coumarate


enzyme_display_name: UniProt P10538

organism: Glycine max

substrate_name: maltopentaose

enzyme: UniProt P10538

substrate: maltopentaose


enzyme_display_name: UniProt Q60032

organism: Thermotoga maritima

substrate_name: beta-Glucan

enzyme: UniProt Q60032

substrate: beta-Glucan


enzyme_display_name: UniProt Q9X273

substrate_name: p-nitrophenyl-beta-D-cellotetraoside

enzyme: UniProt Q9X273

substrate: p-nitrophenyl-beta-D-cellotetraoside


substrate_name: p-nitrophenyl-beta-D-cellopentaoside

substrate: p-nitrophenyl-beta-D-cellopentaoside


organism: Thermoascus aurantiacus

substrate_name: 4-nitrophenyl alpha-L-arabinofuranoside

substrate: 4-nitrophenyl alpha-L-arabinofuranoside


substrate_name: 4-nitrophenyl alpha-L-arabinopyranoside

substrate: 4-nitrophenyl alpha-L-arabinopyranoside


substrate_name: 4-nitrophenyl beta-D-cellobioside

substrate: 4-nitrophenyl beta-D-cellobioside


enzyme_display_name: UniProt P29127

organism: Neocallimastix patriciarum

enzyme: UniProt P29127


organism: Schizophyllum commune

substrate_name: methyl caffeate

substrate: methyl caffeate


substrate_name: methyl ferulate

substrate: methyl ferulate


organism: Talaromyces stipitatus

substrate_name: methyl sinapinate

substrate: methyl sinapinate


proof_lines:
pH 6.0, 37°C
pH 6.0-7.0, 60°C
hydrolysis


organism: Arabidopsis thaliana

substrate_name: palmitoleoyl-CoA

assay_conditions_summary: pH 8; cofactor CoA

substrate: palmitoleoyl-CoA


substrate_name: dodecanoyl-CoA

substrate: dodecanoyl-CoA


enzyme_display_name: protein-tyrosine-phosphatase

organism: Acinetobacter johnsonii

substrate_name: 4-nitrophenyl phosphate

enzyme: protein-tyrosine-phosphatase

substrate: 4-nitrophenyl phosphate


substrate_name: phosphotyrosine

substrate: phosphotyrosine


organism: Aspergillus nidulans


organism: Aspergillus awamori

substrate_name: alpha-naphthylbutyrate

substrate: alpha-naphthylbutyrate


substrate_name: feruloyl arabino-xylo-oligosaccharides

proof_lines: recombinant His-tagged enzyme, pH 7.0, 37°C

substrate: feruloyl arabino-xylo-oligosaccharides


organism: Aspergillus terreus

substrate_name: p-coumaric acid methyl ester

substrate: p-coumaric acid methyl ester


substrate_name: sinapic acid methyl ester

substrate: sinapic acid methyl ester


enzyme_display_name: UniProt P53051

organism: Saccharomyces cerevisiae

substrate_name: methyl alpha-D-glucopyranoside

enzyme: UniProt P53051

substrate: methyl alpha-D-glucopyranoside


substrate_name: 4-nitrophenyl alpha-D-glucopyranoside

proof_lines:
Raw SKiD Env: Temp=30°C, pH=7
Raw SKiD Env: Temp=30°C, pH=6.8

substrate: 4-nitrophenyl alpha-D-glucopyranoside


substrate_name: sucrose

substrate: sucrose


substrate_name: palatinose

substrate: palatinose


substrate_name: isomaltose

substrate: isomaltose


enzyme_display_name: UniProt A0A221ZS22

organism: Ostrinia furnacalis

enzyme: UniProt A0A221ZS22


organism: Aspergillus niger

substrate_name: methyl 3,4-dimethoxycinnamate

substrate: methyl 3,4-dimethoxycinnamate


enzyme_display_name: phosphodiesterase I

substrate_name: 5'-p-nitrophenyladenosine 5'-phosphate

proof_lines: FS-44: 5'-PDase activity of bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase

enzyme: phosphodiesterase I

substrate: 5'-p-nitrophenyladenosine 5'-phosphate


organism: Bacillus subtilis

substrate_name: KRLIEDNE(pY)TARGQ

assay_conditions_summary: pH 8

proof_lines:
pH 8.0, presence of 1 mM Mn2+
pH 8.0

substrate: KRLIEDNE(pY)TARGQ


substrate_name: ethyl ferulate

proof_lines:
pH 6.0, 40°C
in 2.7% dimethylsulfoxide
pH 3.2

substrate: ethyl ferulate


substrate_name: n-propyl ferulate

substrate: n-propyl ferulate


organism: Bos taurus

assay_conditions_summary: pH 7

proof_lines:
pH 7
pH 8
pH 9


enzyme_display_name: palmitoyl[protein] hydrolase

substrate_name: palmitoyl-H-Ras

enzyme: palmitoyl[protein] hydrolase

substrate: palmitoyl-H-Ras


substrate_name: palmitoyl-CoA

substrate: palmitoyl-CoA


enzyme_display_name: aryldialkylphosphatase

organism: Brevundimonas diminuta

substrate_name: paraoxon

assay_conditions_summary: pH 8.5; 303.15 K; buffer 20 mM CHES

proof_lines: pH 8.5, recombinant wild-type enzyme-MBP fusion protein

proof_lines: recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C

enzyme: aryldialkylphosphatase

substrate: paraoxon


proof_lines: pH 8.5, recombinant genetic variants 1.5E-MBP fusion protein

proof_lines: pH 8.5, recombinant genetic variants 1.6F-MBP fusion protein

proof_lines: pH 8.5, recombinant genetic variants 2.S5-MBP fusion protein


substrate_name: bis(4-methylumbelliferyl) phosphate

substrate: bis(4-methylumbelliferyl) phosphate


substrate_name: 4-methylumbelliferyl phenylphosphonate

substrate: 4-methylumbelliferyl phenylphosphonate


substrate_name: parathion

proof_lines: pH 10.5, 20°C, recombinant His6-tagged OPH

substrate: parathion


substrate_name: methylparathion

substrate: methylparathion


substrate_name: diethyl 4-chlorophenyl phosphate

proof_lines: recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C

proof_lines: recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C

substrate: diethyl 4-chlorophenyl phosphate


organism: Escherichia coli

proof_lines: pH 8.0, 37°C, recombinant His6-tagged wild-type OPH


organism: Escherichia coli K-12

substrate_name: chlorpyrifos

substrate: chlorpyrifos


proof_lines:
pH 8.0, 37°C, genetic variant A1467
pH 8.0, 37°C, genetic variant A1030
pH 8.0, 37°C, genetic variant B2136


substrate_name: decanoyl-CoA

assay_conditions_summary: pH 7.3; 310.15 K; cofactor CoA

substrate: decanoyl-CoA


assay_conditions_summary: pH 7.5; 310.15 K; cofactor CoA

proof_lines: pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay


organism: Fusarium oxysporum


organism: Fusarium vanettenii

substrate_name: 4-nitrophenyl butyrate

proof_lines: pH 7.0, 30°C, recombinant enzyme on yeast cell surface

substrate: 4-nitrophenyl butyrate


substrate_name: 4-nitrophenyl acetate

substrate: 4-nitrophenyl acetate


organism: Haemophilus influenzae

substrate_name: KDN 9-phosphate

substrate: KDN 9-phosphate


organism: Homo sapiens

substrate_name: diuridine tetraphosphate

substrate: diuridine tetraphosphate


substrate_name: ADP-ribose

assay_conditions_summary: pH 7.4; 310.15 K; cofactor ADP

substrate: ADP-ribose


substrate_name: 4-nitrophenyl phosphate

proof_lines:
mutant 416AA, PTPalpha, 25°C, pH 7.0
mutant 529AA, PTPalpha, 25°C, pH 7.0

substrate: 4-nitrophenyl phosphate


organism: Fusarium solani

substrate_name: 4-nitrophenyl valerate

assay_conditions_summary: pH 7.5; buffer 14.5 mM Tris-HCl

proof_lines: in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol

substrate: 4-nitrophenyl valerate


substrate_name: 4-nitrophenyl hexanoate

substrate: 4-nitrophenyl hexanoate


substrate_name: Unknown

proof_lines: isoform PTPbeta, mutant N47R/N48D, pH 7.0, 25°C

substrate: Unknown


proof_lines: isoform PTPbeta, mutant N47R/D48N, pH 7.0, 25°C


substrate_name: palmitoyl-peptide

substrate: palmitoyl-peptide


substrate_name: 1-palmitoyl lysophosphatidylcholine

substrate: 1-palmitoyl lysophosphatidylcholine


proof_lines: reaction catalyzed by the full-length enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C

proof_lines: reaction catalyzed by the catalyctic domain of the enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C


substrate_name: bis(4-nitrophenyl) phosphate

substrate: bis(4-nitrophenyl) phosphate


substrate_name: diadenosine 5',5'''-P1,P3-triphosphate

assay_conditions_summary: pH 8.5; 310.15 K; cofactor ADP

proof_lines: in 20 mM Tris-HCl, pH 8.0, 0.5 M NaCl and 1 mM CaCl2, at 37°C

substrate: diadenosine 5',5'''-P1,P3-triphosphate


enzyme_display_name: glycerophosphodiester phosphodiesterase

organism: Klebsiella aerogenes

substrate_name: bis(p-nitrophenyl) phosphate

assay_conditions_summary: pH 9

proof_lines:
pH 9.0, Co(II)-substituted enzyme
pH 10.0, Co(II)-substituted enzyme
pH 11.0, Co(II)-substituted enzyme

enzyme: glycerophosphodiester phosphodiesterase

substrate: bis(p-nitrophenyl) phosphate


substrate_name: p-nitrophenyl phosphate

substrate: p-nitrophenyl phosphate


mutation_type: deletion

proof_lines:
truncated enzyme b-PTP1B(DELTA321)
truncated enzyme PTP1B(DELTA321)


enzyme_display_name: hormone-sensitive lipase

substrate_name: tributanoyl glycerol

enzyme: hormone-sensitive lipase

substrate: tributanoyl glycerol


substrate_name: tripropionyl glycerol

smiles: CCC(=O)C(CO)(C(C(=O)CC)(C(=O)CC)O)O

substrate: tripropionyl glycerol


organism: Mus musculus

substrate_name: p-nitrophenylthymidine 5'-phosphate

substrate: p-nitrophenylthymidine 5'-phosphate


enzyme_display_name: feruloyl esterase

organism: Piromyces sp. 'equi'

enzyme: feruloyl esterase


organism: Rattus norvegicus

substrate_name: trioleoylglycerol

substrate: trioleoylglycerol


substrate_name: dioleoylglycerol

substrate: dioleoylglycerol


organism: Trypanosoma brucei

substrate_name: Raytide peptide

substrate: Raytide peptide


organism: Schizophyllum commune

substrate_name: chlorogenic acid

substrate: chlorogenic acid


substrate_name: methyl 4-hydroxybenzoate

substrate: methyl 4-hydroxybenzoate


organism: uncultured microorganism

substrate_name: methyl p-coumarate

proof_lines:
pH 5.0, 30°C
recombinant enzyme, pH 8.0, 37°C

substrate: methyl p-coumarate


substrate_name: methyl ferulate

substrate: methyl ferulate


organism: Aspergillus awamori

substrate_name: ethyl ferulate

substrate: ethyl ferulate


organism: Aspergillus fumigatus

substrate_name: 4-nitrophenyl butyrate

substrate: 4-nitrophenyl butyrate


substrate_name: 1-naphthyl acetate

proof_lines: in 20% N,N-dimethyl formamide, 50 mM acetate buffer, pH 5, with 0.05 ml enzyme solution, at 37°C for 10 min

substrate: 1-naphthyl acetate


organism: Aspergillus niger

substrate_name: methyl sinapinate

proof_lines:
pH 6.0, 37°C, native enzyme
pH 5.0, 55°C
FAEA produced in Escherichia coli (EcR)

substrate: methyl sinapinate


organism: Arabidopsis thaliana

substrate_name: stearoyl-CoA

proof_lines: pH 8, temperature not specified in the publication

substrate: stearoyl-CoA


organism: Aspergillus terreus


substrate_name: 4-nitrophenyl decanoate

substrate: 4-nitrophenyl decanoate


organism: Bacillus thuringiensis


organism: Bacillus thuringiensis serovar kurstaki

substrate_name: N-hexanoyl-(S)-homoserine lactone

proof_lines: pH 7.4, 28°C, purified detagged recombinant enzyme

substrate: N-hexanoyl-(S)-homoserine lactone


organism: Bos taurus

substrate_name: fructose 2,6-bisphosphate

substrate: fructose 2,6-bisphosphate


proof_lines: with PDPc, a member of the protein phosphatase 2C family


enzyme_display_name: deoxyribonuclease II

substrate_name: supercoiled plasmid DNA

proof_lines: pH 2.6, 3.0% IgG relative content, 18.6% relative DNase activity

proof_lines: 0.1 M NaCl, 11.1% IgG relative content, 25.7% relative DNase activity

proof_lines: 3 M NaCl, 2.0% IgG relative content, 22.3% relative DNase activity

enzyme: deoxyribonuclease II

substrate: supercoiled plasmid DNA


substrate_name: p-nitrophenyl butyrate

substrate: p-nitrophenyl butyrate


substrate_name: cholesteryl oleate

proof_lines: cholesteryl oleate vesicles, 37°C, pH 6.8

proof_lines: cholesteryl oleate emulsion, 37°C, pH 6.8

proof_lines: liquid crystals of cholesteryl oleate, 37°C, pH 6.8

substrate: cholesteryl oleate


enzyme_display_name: 2-lysophosphatidate phosphatase

substrate_name: 1-oleoyl-sn-glycerol 3-phosphate

enzyme: 2-lysophosphatidate phosphatase

substrate: 1-oleoyl-sn-glycerol 3-phosphate


enzyme_display_name: cutinase

organism: Botrytis cinerea

enzyme: cutinase


organism: Daucus carota

substrate_name: glycerophosphocholine

substrate: glycerophosphocholine


organism: Aspergillus oryzae

assay_conditions_summary: pH 7.5; 298.15 K; buffer 14.5 mM Tris-HCl

proof_lines: in 14.5 mM Tris-HCl buffer, pH 7.5, 0.75% glycerol

proof_lines: pH and temperature not specified in the publication


substrate_name: glycerophosphoethanolamine

substrate: glycerophosphoethanolamine


enzyme_display_name: exodeoxyribonuclease V

organism: Escherichia coli

substrate_name: 34-mer ssDNA

proof_lines:
25°C
DNA-dependent ATPase activity
unwinding activity

enzyme: exodeoxyribonuclease V

substrate: 34-mer ssDNA


enzyme_display_name: type II site-specific deoxyribonuclease

substrate_name: DNA

enzyme: type II site-specific deoxyribonuclease

substrate: DNA


substrate_name: DNA junction RC1(2,3')

substrate: DNA junction RC1(2,3')


substrate_name: ATP

proof_lines:
DNA-unwinding activity
DNA-dependent ATPase activity
unwinding activity

substrate: ATP


substrate_name: dATP

substrate: dATP


enzyme_display_name: poly(3-hydroxybutyrate) depolymerase

organism: Cupriavidus necator

proof_lines:
linear 3-hydroxybutyrate oligomer
cyclic 3-hydroxybutyrate oligomer

enzyme: poly(3-hydroxybutyrate) depolymerase


enzyme_display_name: exodeoxyribonuclease (lambda-induced)

substrate_name: Double-stranded DNA

proof_lines:
at 20°C and pH 7.5
at 37°C and pH 7.5
5'-OH blunt end double-stranded DNA

enzyme: exodeoxyribonuclease (lambda-induced)

substrate: Double-stranded DNA


organism: Drosophila melanogaster

substrate_name: 4-nitrophenyl phosphate

substrate: 4-nitrophenyl phosphate


substrate_name: bis(glycerophospho)glycerol

substrate: bis(glycerophospho)glycerol


enzyme_display_name: exodeoxyribonuclease I

substrate_name: single-stranded DNA

assay_conditions_summary: pH 8; 310.15 K; buffer 20 mM Tris-HCl

proof_lines: at 37°C, in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 3 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 10% (v/v) glycerol, 5% (v/v) dimethyl sulfoxide

enzyme: exodeoxyribonuclease I

substrate: single-stranded DNA


organism: Fusarium solani

substrate_name: p-nitrophenylbutanoate

proof_lines:
without surfactant
cutinase I
cutinase II

substrate: p-nitrophenylbutanoate


substrate_name: ColE1 DNA

proof_lines:
EcoRI, activated by Co2+, 37°C
EcoR1, 37°C
EcoRI, pBR322, 37°C

substrate: ColE1 DNA


enzyme_display_name: fructose-2,6-bisphosphate 2-phosphatase

organism: Gallus gallus

substrate_name: Unknown

enzyme: fructose-2,6-bisphosphate 2-phosphatase

substrate: Unknown


substrate_name: 4-nitrophenyl butyrate

substrate: 4-nitrophenyl butyrate


enzyme_display_name: protein-tyrosine-phosphatase

enzyme: protein-tyrosine-phosphatase


substrate_name: fructose 2,6-bisphosphate

substrate: fructose 2,6-bisphosphate


organism: Homo sapiens

proof_lines: pH 5.5, 30°C, PTP1B, mutants M258C, G259Q


proof_lines:
in presence of 1.0 mM Co2+, 37°C
pH 7.0, 37°C, presence of 1 mM Mg2+
in presence of 1.0 mM Mg2+, 37°C


substrate_name: fluorescein diphosphate

substrate: fluorescein diphosphate


substrate_name: palmitoyl-CoA

substrate: palmitoyl-CoA


enzyme_display_name: sn-1-specific diacylglycerol lipase

enzyme: sn-1-specific diacylglycerol lipase


substrate_name: 3,6-fluorescein diphosphate

substrate: 3,6-fluorescein diphosphate


substrate_name: p-nitrophenyl phosphate

verification_status: verified

sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature C215D to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 6

column_label: Table I, row C215D, Km column pH 6, substrate pNPP/p-nitrophenyl phosphate, raw value 1.1 +/- 0.1 mM; central value matches export Km 1.1 mM.

raw_value_unit_evidence: Table I reports C215D Km at pH 6 as 1.1 +/- 0.1 mM.

row_label: C215D PTP-1B

table_label: Table I: Kinetic parameters of C215D and wild-type PTP-1B-mediated pNPP hydrolysis at pH 4.5 and 6

substrate: p-nitrophenyl phosphate

status: verified


proof_lines: MKP-4, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol

proof_lines: MKP-3, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol

proof_lines: MKP-5, in 20 mM Tris-HCl (pH 8.0) containing 2 mM dithiothreitol


proof_lines: pH 7.0, 30°C, isoform PTPH1, mutant H182F


enzyme_display_name: exodeoxyribonuclease III

substrate_name: PUC18 DNA PstI

proof_lines:
31°C and 37°C, 0.05 mM base pairs
41°C, 0.05 mM base pairs

enzyme: exodeoxyribonuclease III

substrate: PUC18 DNA PstI


enzyme_display_name: alpha-glucosidase

substrate_name: maltotriose

enzyme: alpha-glucosidase

substrate: maltotriose


substrate_name: 4-nitrophenyl alpha-D-glucopyranoside

substrate: 4-nitrophenyl alpha-D-glucopyranoside


organism: Mucor javanicus

substrate_name: maltose

substrate: maltose


organism: Mucor racemosus


substrate_name: panose

substrate: panose


substrate_name: isomaltose

substrate: isomaltose


organism: Thermomonospora curvata


enzyme_display_name: UniProt P0CD66

organism: Saccharolobus solfataricus

enzyme: UniProt P0CD66


substrate_name: glycogen

substrate: glycogen


enzyme_display_name: UniProt P10253

enzyme: UniProt P10253


organism: Thermoplasma acidophilum

source_db: oed

source: oed


substrate_name: kojibiose

substrate: kojibiose


substrate_name: maltotetraose

substrate: maltotetraose


enzyme_display_name: beta-glucosidase

organism: Carica papaya

enzyme: beta-glucosidase


organism: Saccharomyces cerevisiae


organism: Trametes versicolor


substrate_name: cellobiose

substrate: cellobiose


enzyme_display_name: UniProt A0A1E1FFN6

organism: uncultured microorganism

substrate_name: 4-nitrophenyl beta-D-fucopyranoside

proof_lines:
Raw SKiD Env: Temp=75°C, pH=5.5
Raw SKiD Env: Temp=60°C, pH=6

enzyme: UniProt A0A1E1FFN6

substrate: 4-nitrophenyl beta-D-fucopyranoside


substrate_name: 4-nitrophenyl beta-D-galactopyranoside

substrate: 4-nitrophenyl beta-D-galactopyranoside


enzyme_display_name: UniProt B8CYA8

organism: Halothermothrix orenii

substrate_name: lactose

enzyme: UniProt B8CYA8

substrate: lactose


enzyme_display_name: UniProt O61594

organism: Spodoptera frugiperda

substrate_name: 4-nitrophenyl beta-D-glucoside

enzyme: UniProt O61594

substrate: 4-nitrophenyl beta-D-glucoside


substrate_name: 4-nitrophenyl beta-D-xyloside

substrate: 4-nitrophenyl beta-D-xyloside


enzyme_display_name: UniProt P49235

organism: Zea mays

substrate_name: Dhurrin

enzyme: UniProt P49235

substrate: Dhurrin


enzyme_display_name: UniProt Q0GC07

organism: Thermotoga neapolitana

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

enzyme: UniProt Q0GC07

substrate: 4-nitrophenyl beta-D-glucopyranoside


enzyme_display_name: UniProt Q92AS9

organism: Listeria innocua Clip11262

substrate_name: laminaritetraose

enzyme: UniProt Q92AS9

substrate: laminaritetraose


substrate_name: sophorose

substrate: sophorose


substrate_name: Laminaripentaose

substrate: Laminaripentaose


substrate_name: laminaritriose

substrate: laminaritriose


substrate_name: laminaribiose

substrate: laminaribiose


substrate_name: sophorotriose

substrate: sophorotriose


enzyme_display_name: UniProt B9K7M5

substrate_name: quercetin 3-O-beta-D-glucopyranoside

enzyme: UniProt B9K7M5

substrate: quercetin 3-O-beta-D-glucopyranoside


enzyme_display_name: UniProt P22073

organism: Paenibacillus polymyxa

enzyme: UniProt P22073


enzyme_display_name: UniProt Q51723

organism: Pyrococcus furiosus

enzyme: UniProt Q51723


enzyme_display_name: UniProt Q9YGA8

organism: Thermosphaera aggregans

substrate_name: 2-nitrophenyl beta-D-glucopyranoside

enzyme: UniProt Q9YGA8

substrate: 2-nitrophenyl beta-D-glucopyranoside


organism: Thermotoga petrophila


enzyme_display_name: Beta-glucosidase 2

organism: Passalora fulva

substrate_name: pNPG

sequence: GVDVLLGQGLLAPRTPFTGEGPSQKSYGTELLSKPNDGK

wild_type_sequence: GVDVLLGQGLLAPRTPFTGEGPSQKSYGTELLSKPNDGK

enzyme: Beta-glucosidase 2

substrate: pNPG


enzyme_display_name: Beta-glucosidase 1

enzyme: Beta-glucosidase 1


enzyme_display_name: alpha-galactosidase

organism: Trametes cinnabarina

substrate_name: p-nitrophenyl alpha-D-galactoside

enzyme: alpha-galactosidase

substrate: p-nitrophenyl alpha-D-galactoside


organism: Micrococcus sp.

substrate_name: 4-nitrophenyl alpha-D-galactoside

substrate: 4-nitrophenyl alpha-D-galactoside


substrate_name: melibiose

substrate: melibiose


organism: Bacillus sp. (in: firmicutes)


substrate_name: stachyose

substrate: stachyose


substrate_name: raffinose

substrate: raffinose


organism: Rhizopus sp.

substrate_name: p-nitrophenyl alpha-D-galactopyranoside

assay_conditions_summary: pH 4.8; 310.15 K; buffer 0.1 M McIlvaine

proof_lines: in 0.1 M McIlvaine buffer (pH 4.8) at 37°C

substrate: p-nitrophenyl alpha-D-galactopyranoside


enzyme_display_name: UniProt Q9ALJ4

organism: Geobacillus stearothermophilus

substrate_name: 4-nitrophenyl alpha-D-galactopyranoside

enzyme: UniProt Q9ALJ4

substrate: 4-nitrophenyl alpha-D-galactopyranoside


enzyme_display_name: beta-galactosidase

organism: Treponema phagedenis

substrate_name: p-nitrophenyl-beta-D-galactoside

enzyme: beta-galactosidase

substrate: p-nitrophenyl-beta-D-galactoside


substrate_name: o-nitrophenyl-beta-D-galactoside

substrate: o-nitrophenyl-beta-D-galactoside


substrate_name: lactose

substrate: lactose


organism: Papiliotrema laurentii


enzyme_display_name: UniProt P00722

organism: Escherichia coli

substrate_name: 4-nitrophenyl beta-D-galactopyranoside

enzyme: UniProt P00722

substrate: 4-nitrophenyl beta-D-galactopyranoside


enzyme_display_name: UniProt P00723

organism: Kluyveromyces marxianus

enzyme: UniProt P00723


enzyme_display_name: UniProt Q2UCU3

organism: Aspergillus oryzae

enzyme: UniProt Q2UCU3


substrate_name: 2-nitrophenyl beta-D-galactoside

substrate: 2-nitrophenyl beta-D-galactoside


enzyme_display_name: alpha-mannosidase

organism: Yarrowia lipolytica

substrate_name: p-nitrophenyl-alpha-D-mannopyranoside

enzyme: alpha-mannosidase

substrate: p-nitrophenyl-alpha-D-mannopyranoside


organism: Aspergillus phoenicis

substrate_name: mannobiose

substrate: mannobiose


enzyme_display_name: protein-tyrosine-phosphatase

organism: Homo sapiens

substrate_name: lysozyme

proof_lines:
enzyme SH-PTP2
enzyme PTP1C
enzyme PTP2C, SH2 domain deleted

enzyme: protein-tyrosine-phosphatase

substrate: lysozyme


enzyme_display_name: aryldialkylphosphatase

substrate_name: dimethyl-paraoxon

sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature H115W to reconstruct the assayed variant sequence.

enzyme: aryldialkylphosphatase

substrate: dimethyl-paraoxon


enzyme_display_name: fructose-2,6-bisphosphate 2-phosphatase

substrate_name: fructose 2,6-bisphosphate

enzyme: fructose-2,6-bisphosphate 2-phosphatase

substrate: fructose 2,6-bisphosphate


substrate_name: phosphotyrosine

assay_conditions_summary: pH 5

substrate: phosphotyrosine


substrate_name: platelet-derived growth factor receptor

proof_lines: value varies with the point ot tyrosine phosphorylation, values for truncated enzyme increased relative to wild-type form

proof_lines: recombinant wild-type enzyme, pH and temperature not specified in the publication

substrate: platelet-derived growth factor receptor


enzyme_display_name: palmitoyl[protein] hydrolase

substrate_name: myristoyl-CoA

enzyme: palmitoyl[protein] hydrolase

substrate: myristoyl-CoA


substrate_name: 4-nitrophenyl phosphate

proof_lines: mutant enzyme D236A/Q314A, at 30°C, in 0.15 M Bis-Tris, pH 6.0

substrate: 4-nitrophenyl phosphate


enzyme_display_name: glycerophosphodiester phosphodiesterase

organism: Klebsiella aerogenes

substrate_name: p-nitrophenyl phosphate

enzyme: glycerophosphodiester phosphodiesterase

substrate: p-nitrophenyl phosphate


organism: Lysinibacillus sp.

substrate_name: N-hexanoyl-L-homoserine lactone

substrate: N-hexanoyl-L-homoserine lactone


substrate_name: bis(p-nitrophenyl phosphate)

proof_lines:
in the presence of 0.1 mM Zn2+
in the presence of 0.1 mM Fe2+

substrate: bis(p-nitrophenyl phosphate)


organism: Leishmania major

proof_lines: pH 6.0, 37°C, recombinant His-tagged enzyme


organism: Mycobacterium tuberculosis

substrate_name: Unknown

proof_lines: at 25°C in 50 mM 3,3-dimethylglutarate buffer, pH 7.0

substrate: Unknown


enzyme_display_name: beta-fructofuranosidase

organism: Carica papaya

substrate_name: sucrose

enzyme: beta-fructofuranosidase

substrate: sucrose


enzyme_display_name: UniProt Q8YWS9

organism: Nostoc sp. PCC 7120 = FACHB-418

proof_lines:
Raw SKiD Env: Temp=30°C, pH=7.9
Raw SKiD Env: Temp=-----, pH=-----

enzyme: UniProt Q8YWS9


organism: Elsholtzia haichowensis


enzyme_display_name: UniProt P13482

substrate_name: trehalose

enzyme: UniProt P13482

substrate: trehalose


enzyme_display_name: UniProt A7XS03

organism: Talaromyces purpureogenus

substrate_name: glycyrrhizin

enzyme: UniProt A7XS03

substrate: glycyrrhizin


organism: Plasmodium falciparum

substrate_name: 3-O-methylfluorescein phosphate

proof_lines: in 50 mM sodium acetate (pH 5.5), 80 mM NaCl, 10 mM dithiothreitol, and 20% glycerol, at 22°C

substrate: 3-O-methylfluorescein phosphate


enzyme_display_name: phosphodiesterase I

organism: Rattus norvegicus

substrate_name: ATP; H2O

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org)

assay_conditions_summary: pH 8.9; 310.15 K; cofactor ATP

proof_lines:
pH 8.9, 37°C
pH 9.6, gp115, AS30D protein
pH 9.6, gp120/gp110, liver protein

enzyme: phosphodiesterase I

substrate: ATP; H2O


substrate_name: Inositol 1,3-bisphosphate

proof_lines:
type I enzyme
type II enzyme

substrate: Inositol 1,3-bisphosphate


enzyme_display_name: xylan 1,4-beta-xylosidase

organism: Thermobifida fusca

substrate_name: p-nitrophenyl beta-D-xylopyranoside

enzyme: xylan 1,4-beta-xylosidase

substrate: p-nitrophenyl beta-D-xylopyranoside


organism: Clostridium sp. BOH3

substrate_name: 4-nitrophenyl alpha-D-xylopyranoside

substrate: 4-nitrophenyl alpha-D-xylopyranoside


enzyme_display_name: UniProt A0A023J5W7

organism: Aspergillus niger

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

proof_lines:
Raw SKiD Env: Temp=40°C, pH=4
Raw SKiD Env: Temp=70°C, pH=4

enzyme: UniProt A0A023J5W7

substrate: 4-nitrophenyl beta-D-glucopyranoside


substrate_name: 4-nitrophenyl alpha-L-arabinofuranoside

substrate: 4-nitrophenyl alpha-L-arabinofuranoside


substrate_name: 4-nitrophenyl alpha-L-arabinopyranoside

proof_lines:
Raw SKiD Env: Temp=70°C, pH=4
Raw SKiD Env: Temp=40°C, pH=4

substrate: 4-nitrophenyl alpha-L-arabinopyranoside


substrate_name: 3,4-dinitrophenyl beta-D-xylopyranoside

substrate: 3,4-dinitrophenyl beta-D-xylopyranoside


enzyme_display_name: UniProt A2ICH1

organism: Thermoanaerobacterium saccharolyticum

enzyme: UniProt A2ICH1


substrate_name: xylobiose

substrate: xylobiose


enzyme_display_name: Probable exo-1,4-beta-xylosidase xlnD

organism: Aspergillus japonicus

enzyme: Probable exo-1,4-beta-xylosidase xlnD


enzyme_display_name: UniProt G9M5R4

organism: Lentinula edodes

substrate_name: Laminarin

enzyme: UniProt G9M5R4

substrate: Laminarin


enzyme_display_name: alpha-L-rhamnosidase

organism: Dictyoglomus thermophilum

substrate_name: 4-nitrophenyl alpha-L-rhamnopyranoside

source_license: CC BY 4.0; CC BY-NC-ND 4.0

sequence_source: multiple

proof_lines:
pH 5.0, 37°C
Raw SKiD Env: Temp=37°C, pH=5

source_databases_merged: brenda skid

enzyme: alpha-L-rhamnosidase

substrate: 4-nitrophenyl alpha-L-rhamnopyranoside


enzyme_display_name: UniProt Q8A916

organism: Bacteroides thetaiotaomicron

enzyme: UniProt Q8A916


enzyme_display_name: protein-tyrosine-phosphatase

substrate_name: L-Phosphotyrosine; H2O

proof_lines:
pH 7.5, 22°C, PTPB1
pH 7.5, 22°C, TCPTP
above, pH 7.5, 22°C, CD45 and LAR

enzyme: protein-tyrosine-phosphatase

substrate: L-Phosphotyrosine; H2O


enzyme_display_name: hormone-sensitive lipase

substrate_name: Unknown

enzyme: hormone-sensitive lipase

substrate: Unknown


enzyme_display_name: fructose-2,6-bisphosphate 2-phosphatase

substrate_name: fructose 2,6-bisphosphate

enzyme: fructose-2,6-bisphosphate 2-phosphatase

substrate: fructose 2,6-bisphosphate


enzyme_display_name: UniProt F6BL85

organism: Thermoanaerobacterium xylanolyticum

substrate_name: laminaribiose

enzyme: UniProt F6BL85

substrate: laminaribiose


substrate_name: 4-nitrophenyl beta-D-glucoside

substrate: 4-nitrophenyl beta-D-glucoside


substrate_name: cellobiose

substrate: cellobiose


enzyme_display_name: UniProt Q6UVM5

organism: Xanthomonas axonopodis

substrate_name: sucrose

proof_lines:
Raw SKiD Env: Temp=-----, pH=-----
Raw SKiD Env: Temp=30°C, pH=7.5

enzyme: UniProt Q6UVM5

substrate: sucrose


enzyme_display_name: alpha-N-acetylgalactosaminidase

organism: Homo sapiens

substrate_name: 4-nitrophenyl N-acetyl-D-galactosamine

enzyme: alpha-N-acetylgalactosaminidase

substrate: 4-nitrophenyl N-acetyl-D-galactosamine


organism: Saccharolobus solfataricus

substrate_name: Holliday junction 3 in DNA

proof_lines: at 30°C, steady-state approximation under maximal velocity conditions at 15 mM MgCl2

substrate: Holliday junction 3 in DNA


enzyme_display_name: aryldialkylphosphatase

substrate_name: methyl paraoxon

assay_conditions_summary: pH 7; buffer 100 mM sodium phosphate

proof_lines: recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C

enzyme: aryldialkylphosphatase

substrate: methyl paraoxon


organism: Saccharomyces cerevisiae

substrate_name: D-loop junction in DNA

substrate: D-loop junction in DNA


organism: Sus scrofa

substrate_name: p-nitrophenylthymidine 5'-phosphate

substrate: p-nitrophenylthymidine 5'-phosphate


organism: Staphylococcus aureus

substrate_name: 4-nitrophenyl phosphate

proof_lines:
pH 6.2, 37°C, PtpA
pH 6.2, 37°C, PtpB

substrate: 4-nitrophenyl phosphate


organism: Streptococcus pneumoniae D39


organism: Rattus norvegicus

substrate_name: cholesteryl oleate

substrate: cholesteryl oleate


enzyme_display_name: acyl-CoA hydrolase

organism: Streptomyces sp.

substrate_name: acetyl-S-N-acetylcysteamine

enzyme: acyl-CoA hydrolase

substrate: acetyl-S-N-acetylcysteamine


enzyme_display_name: cutinase

organism: Thermobifida fusca

substrate_name: 4-nitrophenyl acetate

proof_lines: pH and temperature not specified in the publication

enzyme: cutinase

substrate: 4-nitrophenyl acetate


enzyme_display_name: UniProt Q8XM24

organism: Clostridium perfringens

enzyme: UniProt Q8XM24


enzyme_display_name: UniProt A0A0H2YQB3

enzyme: UniProt A0A0H2YQB3


enzyme_display_name: UniProt E3PQQ9

organism: Paenibacillus thiaminolyticus

enzyme: UniProt E3PQQ9


enzyme_display_name: UniProt Q06GJ0

organism: Ostrinia furnacalis

substrate_name: chitobiose

enzyme: UniProt Q06GJ0

substrate: chitobiose


enzyme_display_name: UniProt P06865

enzyme: UniProt P06865


enzyme_display_name: UniProt Q8DRL6

organism: Streptococcus pneumoniae

enzyme: UniProt Q8DRL6


enzyme_display_name: cyclomaltodextrinase

organism: Anoxybacillus flavithermus subsp. flavithermus

substrate_name: gamma-cyclodextrin

enzyme: cyclomaltodextrinase

substrate: gamma-cyclodextrin


substrate_name: beta-cyclodextrin

substrate: beta-cyclodextrin


substrate_name: alpha-cyclodextrin

substrate: alpha-cyclodextrin


organism: Flavobacterium sp.


organism: Bacillus subtilis

substrate_name: maltoheptaose

substrate: maltoheptaose


organism: Lysinibacillus sphaericus


substrate_name: maltohexaose

substrate: maltohexaose


substrate_name: maltotetraose

substrate: maltotetraose


substrate_name: maltotriose

substrate: maltotriose


enzyme_display_name: UniProt A3DEX4

organism: Acetivibrio thermocellus

enzyme: UniProt A3DEX4


enzyme_display_name: UniProt E2GHW5

organism: Podospora anserina

enzyme: UniProt E2GHW5


enzyme_display_name: UniProt O54161

organism: Streptomyces coelicolor

enzyme: UniProt O54161


enzyme_display_name: UniProt O69262

organism: Thermobacillus xylanilyticus

substrate_name: 4-nitrophenyl alpha-L-arabinofuranoside

proof_lines:
Raw SKiD Env: Temp=45°C, pH=7
Raw SKiD Env: Temp=60°C, pH=5.8

enzyme: UniProt O69262

substrate: 4-nitrophenyl alpha-L-arabinofuranoside


substrate_name: p-nitrophenyl-alpha-L-arabinofuranose

proof_lines:
Raw SKiD Env: Temp=60°C, pH=-----
Raw SKiD Env: Temp=-----, pH=-----

substrate: p-nitrophenyl-alpha-L-arabinofuranose


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature L352M to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature R69H to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature R69H/G179F/L352M to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature R69H/L352M to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature R69H/N216W/L352M to reconstruct the assayed variant sequence.


enzyme_display_name: poly(ethylene terephthalate) hydrolase

substrate_name: Unknown

enzyme: poly(ethylene terephthalate) hydrolase

substrate: Unknown


organism: Thermobifida alba


enzyme_display_name: protein-tyrosine-phosphatase

organism: Thermus thermophilus

proof_lines:
pH 3.8, 30°C, recombinant enzyme
pH 3.8, 75°C, recombinant enzyme

enzyme: protein-tyrosine-phosphatase


organism: Thermothielavioides terrestris

substrate_name: lignin-rich pellet

substrate: lignin-rich pellet


organism: Triticum aestivum

substrate_name: Inositol hexakisphosphate

substrate: Inositol hexakisphosphate


organism: Trypanosoma cruzi

substrate_name: 4-nitrophenyl phosphate

proof_lines:
pH 7.5, 22°C, recombinant enzyme
pH 7.5

substrate: 4-nitrophenyl phosphate


organism: Yersinia enterocolitica

proof_lines: value strongly dependent on ionic strength and pH


enzyme_display_name: UniProt Q53EH2

organism: Clostridium cellulovorans

substrate_name: cellotriose

enzyme: UniProt Q53EH2

substrate: cellotriose


substrate_name: cellotetraose

substrate: cellotetraose


substrate_name: cellobiose

substrate: cellobiose


enzyme_display_name: UniProt P35475

organism: Homo sapiens

substrate_name: 4-methylumbelliferyl alpha-L-iduronide

proof_lines:
Raw SKiD Env: Temp=37°C, pH=3.5
Raw SKiD Env: Temp=32°C, pH=4.2
Raw SKiD Env: Temp=37°C, pH=2.8

enzyme: UniProt P35475

substrate: 4-methylumbelliferyl alpha-L-iduronide


enzyme_display_name: UniProt Q99036

organism: Trichoderma reesei

substrate_name: Mannotetraose

source_databases_merged: skid uniprot

enzyme: UniProt Q99036

substrate: Mannotetraose


substrate_name: mannohexaose

substrate: mannohexaose


substrate_name: Mannopentaose

substrate: Mannopentaose


enzyme_display_name: mannan endo-1,4-beta-mannosidase

substrate_name: 4-methylumbelliferyl mannotrioside

sequence_variant_note: Resolved sequence already matches the mutant residue(s) after applying an inferred residue-numbering offset +2, so it is treated as a source-provided variant sequence.

enzyme: mannan endo-1,4-beta-mannosidase

substrate: 4-methylumbelliferyl mannotrioside


substrate_name: locust bean gum

substrate: locust bean gum


enzyme_display_name: fructose-2,6-bisphosphate 2-phosphatase

organism: Rattus norvegicus

substrate_name: D-Fructose 2,6-bisphosphate; H2O

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org)

enzyme: fructose-2,6-bisphosphate 2-phosphatase

substrate: D-Fructose 2,6-bisphosphate; H2O


substrate_name: H2O; D-myo-Inositol 1,3-bisphosphate

substrate: H2O; D-myo-Inositol 1,3-bisphosphate


organism: Yersinia sp. (in: enterobacteria)


enzyme_display_name: UniProt P11546

organism: Lactococcus lactis

substrate_name: o-nitrophenyl-beta-D-galactoside

enzyme: UniProt P11546

substrate: o-nitrophenyl-beta-D-galactoside


enzyme_display_name: pyrethroid hydrolase

organism: Klebsiella sp.

substrate_name: 4-nitrophenyl acetate

enzyme: pyrethroid hydrolase

substrate: 4-nitrophenyl acetate


substrate_name: 4-nitrophenyl butanoate

substrate: 4-nitrophenyl butanoate


substrate_name: 4-nitrophenyl hexanoate

substrate: 4-nitrophenyl hexanoate


substrate_name: 4-nitrophenyl dodecanoate

substrate: 4-nitrophenyl dodecanoate


substrate_name: 4-nitrophenyl octanoate

substrate: 4-nitrophenyl octanoate


substrate_name: 4-nitrophenyl propanoate

substrate: 4-nitrophenyl propanoate


enzyme_display_name: UniProt P07986

organism: Cellulomonas fimi

substrate_name: 4-nitrophenyl beta-D-cellobioside

enzyme: UniProt P07986

substrate: 4-nitrophenyl beta-D-cellobioside


enzyme_display_name: UniProt Q65MI2

organism: Bacillus licheniformis

substrate_name: 4-nitrophenyl alpha-D-glucopyranoside

enzyme: UniProt Q65MI2

substrate: 4-nitrophenyl alpha-D-glucopyranoside


substrate_name: alpha,alpha-trehalose 6-phosphate

substrate: alpha,alpha-trehalose 6-phosphate


enzyme_display_name: UniProt Q93HW0

organism: Streptococcus pneumoniae

substrate_name: Fmoc-Asn(GlcNAc)-OH

enzyme: UniProt Q93HW0

substrate: Fmoc-Asn(GlcNAc)-OH


enzyme_display_name: UniProt G5ELM1

organism: Bifidobacterium bifidum

substrate_name: Tn antigen

enzyme: UniProt G5ELM1

substrate: Tn antigen


organism: Mucor hiemalis


proof_lines: pH 6.5, 30°C, recombinant mutant Y217F/N175Q


organism: Catharanthus roseus

substrate_name: dolichantoside

substrate: dolichantoside


substrate_name: palicoside

substrate: palicoside


enzyme_display_name: exo-poly-alpha-digalacturonosidase

organism: Trametes sanguinea

enzyme: exo-poly-alpha-digalacturonosidase


substrate_name: Pectin

substrate: Pectin


substrate_name: pectate

substrate: pectate


enzyme_display_name: UniProt Q24451

organism: Drosophila melanogaster

substrate_name: 5-fluoro-beta-L-gulosyl fluoride

enzyme: UniProt Q24451

substrate: 5-fluoro-beta-L-gulosyl fluoride


enzyme_display_name: UniProt Q99YP5

organism: Streptococcus pyogenes M1 GAS

enzyme: UniProt Q99YP5


organism: Unknown

substrate_name: Unknown

source_db: unknown

source_license: see source

substrate: Unknown

source: unknown


enzyme_display_name: cellulose 1,4-beta-cellobiosidase (non-reducing end)

organism: Fomitopsis pinicola

assay_conditions_summary: pH 5; 323.15 K; buffer 100 mM sodium acetate

proof_lines: in 100 mM sodium acetate buffer (pH 5.0) at 50°C


substrate_name: cellobiose

enzyme: cellulose 1,4-beta-cellobiosidase (non-reducing end)

substrate: cellobiose


enzyme_display_name: mannan 1,4-mannobiosidase

organism: Aeromonas hydrophila

substrate_name: Mannopentaose

enzyme: mannan 1,4-mannobiosidase

substrate: Mannopentaose


substrate_name: Mannotetraose

substrate: Mannotetraose


substrate_name: mannobiose

substrate: mannobiose


organism: Xanthomonas citri

substrate_name: mannohexaose

substrate: mannohexaose


enzyme_display_name: UniProt P04957

organism: Bacillus subtilis

substrate_name: lichenan

enzyme: UniProt P04957

substrate: lichenan


enzyme_display_name: UniProt Q9X274

organism: Thermotoga maritima

enzyme: UniProt Q9X274


organism: Microbacterium dextranolyticum

substrate_name: kojibiose

mutation_signature: truncated mutant enzyme MdDDE45-913

mutation_type: truncation

proof_lines: pH 5.6, 30°C, truncated mutant enzyme MdDDE45-913

substrate: kojibiose


mutation_signature: truncated mutant enzyme MdDDE45-1175

proof_lines: pH 5.6, 30°C, truncated mutant enzyme MdDDE45-1175


substrate_name: kojitriose

substrate: kojitriose


substrate_name: S-32 alpha-glucan

substrate: S-32 alpha-glucan


substrate_name: B-1299 alpha-glucan

substrate: B-1299 alpha-glucan


organism: Strychnos mellodora

substrate_name: strictosidine

substrate: strictosidine


substrate_name: dolichantoside

substrate: dolichantoside


enzyme_display_name: glucan 1,4-alpha-maltotriohydrolase

organism: Kitasatospora sp. MK-1785

substrate_name: maltotetraose

enzyme: glucan 1,4-alpha-maltotriohydrolase

substrate: maltotetraose


substrate_name: amylose

substrate: amylose


substrate_name: maltopentaose

substrate: maltopentaose


enzyme_display_name: steryl-beta-glucosidase

organism: Cryptococcus neoformans

substrate_name: resorufin 3-O-beta-D-glucoside

enzyme: steryl-beta-glucosidase

substrate: resorufin 3-O-beta-D-glucoside


substrate_name: 4-methylumbelliferyl beta-D-glucoside

substrate: 4-methylumbelliferyl beta-D-glucoside


substrate_name: 4-nitrophenyl beta-D-glucoside

substrate: 4-nitrophenyl beta-D-glucoside


organism: Natronococcus sp.

substrate_name: gamma-cyclodextrin

proof_lines: pH 9, 50°C, Km-value shown as glucose equivalents

substrate: gamma-cyclodextrin


substrate_name: starch

proof_lines: pH 9, 50°C, DP, 17, Km-value shown as glucose equivalents

proof_lines: pH 9, 50°C, soluble, Km-value shown as glucose equivalents

substrate: starch


substrate_name: glycogen

proof_lines: pH 9, 50°C, from oyster, Km-value shown as glucose equivalents

substrate: glycogen


enzyme_display_name: vicianin beta-glucosidase

organism: Davallia trichomanoides

substrate_name: (R)-Prunasin

enzyme: vicianin beta-glucosidase

substrate: (R)-Prunasin


substrate_name: p-nitrophenyl-beta-D-glucopyranoside

substrate: p-nitrophenyl-beta-D-glucopyranoside


substrate_name: (R)-Vicianin

substrate: (R)-Vicianin


enzyme_display_name: endoglycosylceramidase

organism: Prescottella equi

substrate_name: 4-nitrophenyl beta-D-galactopyranoside

proof_lines: pH 6.5, 37°C, recombinant mutant W372A, calculated by BRENDA

enzyme: endoglycosylceramidase

substrate: 4-nitrophenyl beta-D-galactopyranoside


proof_lines: pH 6.5, 37°C, recombinant mutant H382S, calculated by BRENDA


proof_lines: pH 6.5, 37°C, recombinant mutant H382E, calculated by BRENDA


proof_lines: pH 6.5, 37°C, recombinant mutant H382Q, calculated by BRENDA


proof_lines: pH 6.5, 37°C, recombinant mutant H382A, calculated by BRENDA


proof_lines: pH 6.5, 37°C, recombinant mutant R185A, calculated by BRENDA


proof_lines: pH 6.5, 37°C, recombinant mutant H382G, calculated by BRENDA


proof_lines: pH 6.5, 37°C, recombinant mutant R185W, calculated by BRENDA


proof_lines: pH 6.5, 37°C, recombinant mutant R185F, calculated by BRENDA


proof_lines: pH 6.5, 37°C, recombinant wild-type enzyme, calculated by BRENDA


enzyme_display_name: oligoxyloglucan beta-glycosidase

organism: Aspergillus oryzae

sequence_variant_note: Resolved sequence already matches the mutant residue(s), so it is treated as a source-provided variant sequence.

enzyme: oligoxyloglucan beta-glycosidase


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature W515F with inferred residue-numbering offset -8 to reconstruct the assayed variant sequence.


proof_lines: pH 4.5, 60°C, recombinant wild-type enzyme


enzyme_display_name: coniferin beta-glucosidase

organism: Agrobacterium tumefaciens

substrate_name: Salicin

enzyme: coniferin beta-glucosidase

substrate: Salicin


substrate_name: p-nitrophenyl beta-D-glucopyranoside

substrate: p-nitrophenyl beta-D-glucopyranoside


substrate_name: p-nitrophenyl beta-D-fucopyranoside

substrate: p-nitrophenyl beta-D-fucopyranoside


substrate_name: p-nitrophenyl beta-D-xylopyranoside

substrate: p-nitrophenyl beta-D-xylopyranoside


substrate_name: coniferin

substrate: coniferin


substrate_name: p-nitrophenyl alpha-L-arabinofuranoside

substrate: p-nitrophenyl alpha-L-arabinofuranoside


substrate_name: o-nitrophenyl beta-D-glucopyranoside

substrate: o-nitrophenyl beta-D-glucopyranoside


substrate_name: arbutin

substrate: arbutin


substrate_name: Unknown

substrate: Unknown


enzyme_display_name: endo-alpha-sialidase

organism: Vectrevirus K1E

substrate_name: polysialic acid

enzyme: endo-alpha-sialidase

substrate: polysialic acid


mutation_signature: Trunc N45


proof_lines:
37°C, pH 6.5, DELTA P-loop
37°C, pH 6.5


organism: Cicer arietinum

substrate_name: formononetin 7-beta-glucoside

substrate: formononetin 7-beta-glucoside


substrate_name: 4-methylumbelliferyl beta-D-glucoside

proof_lines: ferulic acid p-beta-glucoside, 4-nitrophenyl beta-D-glucoside

substrate: 4-methylumbelliferyl beta-D-glucoside


substrate_name: Picein

substrate: Picein


substrate_name: 2-nitrophenyl beta-D-galactoside

substrate: 2-nitrophenyl beta-D-galactoside


substrate_name: syringin

substrate: syringin


organism: Arabidopsis thaliana

substrate_name: p-coumaryl beta-D-glucoside

substrate: p-coumaryl beta-D-glucoside


organism: Picea abies

proof_lines: roots hypocotyls


proof_lines: 2-nitrophenyl beta-D-glucoside, 2-nitrophenyl beta-D-galactoside, hypocotyls


substrate_name: 4-nitrophenyl beta-D-glucoside

substrate: 4-nitrophenyl beta-D-glucoside


proof_lines:
isoform EGCrP1, at pH 6.0 and 37°C
isoform EGCrP2, at pH 6.0 and 37°C


substrate_name: resorufin

substrate: resorufin


organism: Oryza sativa Japonica Group

substrate_name: 4-coumaryl alcohol glucoside

proof_lines: recombinant enzyme, pH and temperature not specified in the publication

substrate: 4-coumaryl alcohol glucoside


enzyme_display_name: UniProt B3PKK9

organism: Cellvibrio japonicus

substrate_name: hydroxyethyl cellulose

enzyme: UniProt B3PKK9

substrate: hydroxyethyl cellulose


substrate_name: amylose

substrate: amylose


substrate_name: maltotetraose

substrate: maltotetraose


substrate_name: maltopentaose

substrate: maltopentaose


organism: Citrobacter freundii

substrate_name: paragloboside

substrate: paragloboside


substrate_name: lacto-N-tetraose

substrate: lacto-N-tetraose


organism: Bifidobacterium longum subsp. infantis


substrate_name: 4-nitrophenyl alpha-L-fucopyranoside

substrate: 4-nitrophenyl alpha-L-fucopyranoside


organism: Streptococcus pyogenes


organism: Xanthomonas citri

substrate_name: beta-mannan

substrate: beta-mannan


substrate_name: mannohexaose

substrate: mannohexaose


organism: Pyrus x bretschneideri


substrate_name: coniferin

substrate: coniferin


enzyme_display_name: UniProt Q6LUT2

organism: Photobacterium profundum

substrate_name: chitobiose

enzyme: UniProt Q6LUT2

substrate: chitobiose


enzyme_display_name: UniProt Q76HN4

organism: Thermococcus kodakarensis

substrate_name: chitotriose

enzyme: UniProt Q76HN4

substrate: chitotriose


substrate_name: chitotetraose

substrate: chitotetraose


substrate_name: chitohexaose

substrate: chitohexaose


substrate_name: chitopentaose

substrate: chitopentaose


enzyme_display_name: UniProt Q0TR53

organism: Clostridium perfringens

enzyme: UniProt Q0TR53


enzyme_display_name: UniProt Q89ZI2

organism: Bacteroides thetaiotaomicron

enzyme: UniProt Q89ZI2


enzyme_display_name: beta-apiosyl-beta-glucosidase

substrate_name: biochanin A 7-O-beta-D-glucopyranoside

proof_lines:
isoenzyme R3
isoenzyme R3, isoenzyme L1
isoenzyme H1

enzyme: beta-apiosyl-beta-glucosidase

substrate: biochanin A 7-O-beta-D-glucopyranoside


substrate_name: apigenin 7-O-beta-D-glucopyranoside

substrate: apigenin 7-O-beta-D-glucopyranoside


substrate_name: 4-methylumbelliferyl beta-D-glucoside

proof_lines:
isoenzyme R3, isoenzyme L1
isoenzyme H1

substrate: 4-methylumbelliferyl beta-D-glucoside


organism: Prescottella equi

substrate_name: ganglioside Gb3Cer

proof_lines:
isoform EHCase II, pH 5.5 and 37°C
pH 6.0, 37°C
isoform EHCase I, pH 5.5 and 37°C

substrate: ganglioside Gb3Cer


substrate_name: ganglioside GM1a

proof_lines:
isoform EHCase II, pH 5.5 and 37°C
isoform EHCase I, pH 5.5 and 37°C

substrate: ganglioside GM1a


enzyme_display_name: oligosaccharide reducing-end xylanase

organism: Pseudoalteromonas haloplanktis

substrate_name: oat spelt xylan

enzyme: oligosaccharide reducing-end xylanase

substrate: oat spelt xylan


substrate_name: xyloheptaose

substrate: xyloheptaose


substrate_name: xylooctaose

substrate: xylooctaose


organism: uncultured bacterium


substrate_name: xylopentaose

substrate: xylopentaose


enzyme_display_name: iota-carrageenase

organism: Alteromonas fortis

substrate_name: iota-carrageenan

proof_lines: mutant enzyme H281A, at 40°C in 10 mM Tris, pH 8.0, and 100 mM NaCl

enzyme: iota-carrageenase

substrate: iota-carrageenan


proof_lines: mutant enzyme E310Q, at 40°C in 10 mM Tris, pH 8.0, and 100 mM NaCl


proof_lines: wild type enzyme, at 40°C in 10 mM Tris, pH 8.0, and 100 mM NaCl


enzyme_display_name: cellulose 1,4-beta-cellobiosidase (non-reducing end)

organism: Mycothermus thermophilus

substrate_name: alpha-cellobiosyl fluoride

substrate: alpha-cellobiosyl fluoride


substrate_name: beta-cellobiosyl fluoride

enzyme: cellulose 1,4-beta-cellobiosidase (non-reducing end)

substrate: beta-cellobiosyl fluoride


organism: Orpinomyces sp.

substrate_name: Barley beta-glucan

proof_lines: 105-residue N-terminal truncation mutant, pH 6.0, 45°C

substrate: Barley beta-glucan


mutation_signature: 17-residue N-terminal truncation mutant

proof_lines: 17-residue N-terminal truncation mutant, pH 6.0, 45°C


organism: Chrysosporium lucknowense

substrate_name: beta-Glucan

substrate: beta-Glucan


substrate_name: xyloglucan

substrate: xyloglucan


enzyme_display_name: UniProt A0A088T0J9

organism: Geotrichum candidum

substrate_name: 4-nitrophenyl beta-D-cellobioside

enzyme: UniProt A0A088T0J9

substrate: 4-nitrophenyl beta-D-cellobioside


enzyme_display_name: UniProt F0ZJZ1

organism: Dictyostelium purpureum

substrate_name: 4-nitrophenyl beta-D-lactoside

enzyme: UniProt F0ZJZ1

substrate: 4-nitrophenyl beta-D-lactoside


enzyme_display_name: UniProt F1CYZ0

organism: Talaromyces funiculosus

substrate_name: 4-nitrophenyl beta-D-lactopyranoside

enzyme: UniProt F1CYZ0

substrate: 4-nitrophenyl beta-D-lactopyranoside


enzyme_display_name: UniProt G2Q665

organism: Thermothelomyces thermophilus

enzyme: UniProt G2Q665


enzyme_display_name: UniProt Q55FE6

organism: Dictyostelium discoideum

proof_lines:
Raw SKiD Env: Temp=40°C, pH=5
Raw SKiD Env: Temp=45°C, pH=5

enzyme: UniProt Q55FE6


enzyme_display_name: UniProt Q8J0K6

organism: Melanocarpus albomyces

substrate_name: 2-chloro-4-nitrophenyl beta-D-lactoside

enzyme: UniProt Q8J0K6

substrate: 2-chloro-4-nitrophenyl beta-D-lactoside


enzyme_display_name: UniProt Q8TFL9

organism: Rasamsonia emersonii

substrate_name: 4-methylumbelliferyl beta-D-lactoside

proof_lines:
Raw SKiD Env: Temp=60°C, pH=5
Raw SKiD Env: Temp=45°C, pH=5
Raw SKiD Env: Temp=22°C, pH=5

enzyme: UniProt Q8TFL9

substrate: 4-methylumbelliferyl beta-D-lactoside


enzyme_display_name: UniProt B3PBD9

organism: Cellvibrio japonicus

substrate_name: Isoprimeverose

enzyme: UniProt B3PBD9

substrate: Isoprimeverose


enzyme_display_name: UniProt P31434

organism: Escherichia coli

enzyme: UniProt P31434


organism: Bacteroides fragilis


organism: Tabernaemontana divaricata

substrate_name: strictosidine

proof_lines:
enzyme form SGIII
above, enzyme form SGII

substrate: strictosidine


organism: Mediterraneibacter gnavus

substrate_name: blood group A II tetrasaccharide

proof_lines: recombinant enzyme, calculated by BRENDA, pH 6.0, 37°C

substrate: blood group A II tetrasaccharide


enzyme_display_name: UniProt Q9Y223

organism: Homo sapiens

substrate_name: UDP-N-acetyl-alpha-D-glucosamine

enzyme: UniProt Q9Y223

substrate: UDP-N-acetyl-alpha-D-glucosamine


enzyme_display_name: UniProt E8MGH8

organism: Bifidobacterium longum

enzyme: UniProt E8MGH8


organism: Saccharolobus solfataricus

substrate_name: 4-nitrophenyl alpha-D-mannoside

assay_conditions_summary: pH 6.5; 338.15 K; buffer 50 mM sodium phosphate

proof_lines: recombinant enzyme, in 50 mM sodium phosphate buffer, pH 6.5, at 65°C

proof_lines: native enzyme, in 50 mM sodium phosphate buffer, pH 6.5, at 65°C

substrate: 4-nitrophenyl alpha-D-mannoside


organism: Flavobacterium sp.

substrate_name: B-1298 soluble dextran

substrate: B-1298 soluble dextran


enzyme_display_name: glucan 1,4-alpha-maltotriohydrolase

organism: Microbacterium imperiale

substrate_name: amylopectin

enzyme: glucan 1,4-alpha-maltotriohydrolase

substrate: amylopectin


enzyme_display_name: ginsenoside Rb1 beta-glucosidase

organism: Pseudothermotoga thermarum

substrate_name: 4-nitrophenyl beta-D-glucopyranoside

enzyme: ginsenoside Rb1 beta-glucosidase

substrate: 4-nitrophenyl beta-D-glucopyranoside


organism: Canavalia ensiformis

substrate_name: Unknown

proof_lines: pH and temperature not specified in the publication

substrate: Unknown


enzyme_display_name: endo-alpha-N-acetylgalactosaminidase

organism: Bifidobacterium bifidum

enzyme: endo-alpha-N-acetylgalactosaminidase


substrate_name: Tn antigen

substrate: Tn antigen


enzyme_display_name: oligoxyloglucan beta-glycosidase

organism: Aspergillus oryzae

enzyme: oligoxyloglucan beta-glycosidase


enzyme_display_name: UniProt Q14697

substrate_name: p-nitrophenyl-alpha-D-glucopyranoside

enzyme: UniProt Q14697

substrate: p-nitrophenyl-alpha-D-glucopyranoside


organism: Oerskovia sp.


organism: Thermobifida fusca

substrate_name: tamarind xyloglucan

substrate: tamarind xyloglucan


organism: Aspergillus phoenicis

proof_lines:
NaBH4 reduced
pH 5, 37°C


enzyme_display_name: maltose-6'-phosphate glucosidase

organism: Enterococcus faecalis

substrate_name: maltose 6'-phosphate

enzyme: maltose-6'-phosphate glucosidase

substrate: maltose 6'-phosphate


organism: Triticum aestivum

substrate_name: DIBOA glucoside; H2O

substrate: DIBOA glucoside; H2O


substrate_name: DIMBOA glucoside; H2O

substrate: DIMBOA glucoside; H2O


enzyme_display_name: polymannuronate hydrolase

organism: Pseudomonas aeruginosa

substrate_name: polymannuronic acid exopolysaccharide

proof_lines: 37°C, pH 7.5, from Pseudomonas aeruginosa, strain PM1

enzyme: polymannuronate hydrolase

substrate: polymannuronic acid exopolysaccharide


enzyme_display_name: endoglycosylceramidase

organism: Corynebacterium sp.

proof_lines: 4-nitrophenyllactoside derivative possessing an N-acyl chain, chromogenic substrate C16ampNPLac, pH 5

enzyme: endoglycosylceramidase


organism: Aspergillus niger

substrate_name: alpha-L-fucosyl asialo-agalacto-fetuin

substrate: alpha-L-fucosyl asialo-agalacto-fetuin


enzyme_display_name: avenacosidase

organism: Avena sativa

enzyme: avenacosidase


organism: Tannerella forsythia

substrate_name: 4-nitrophenyl alpha-L-fucopyranoside

substrate: 4-nitrophenyl alpha-L-fucopyranoside


enzyme_display_name: purine nucleosidase

substrate_name: guanosine

enzyme: purine nucleosidase

substrate: guanosine


substrate_name: adenosine

substrate: adenosine


enzyme_display_name: UniProt Q97WH6

organism: Saccharolobus solfataricus

substrate_name: Inosine

enzyme: UniProt Q97WH6

substrate: Inosine


enzyme_display_name: UniProt Q9GPQ4

organism: Trypanosoma vivax

enzyme: UniProt Q9GPQ4


proof_lines:
Raw SKiD Env: Temp=35°C, pH=7.2
Raw SKiD Env: Temp=-----, pH=7


substrate_name: uridine

substrate: uridine


enzyme_display_name: UniProt B6T563

organism: Zea mays

substrate_name: Xanthosine

enzyme: UniProt B6T563

substrate: Xanthosine


enzyme_display_name: UniProt Q57ZL6

organism: Trypanosoma brucei brucei

substrate_name: 2-aminopurine riboside

enzyme: UniProt Q57ZL6

substrate: 2-aminopurine riboside


assay_conditions_summary: pH 7


substrate_name: cytidine

substrate: cytidine


enzyme_display_name: UniProt A9TXA6

organism: Physcomitrium patens

enzyme: UniProt A9TXA6


enzyme_display_name: glycyrrhizin hydrolase

organism: Anas platyrhynchos

substrate_name: glycyrrhizinic acid

enzyme: glycyrrhizin hydrolase

substrate: glycyrrhizinic acid


organism: Paenibacillus sp.

substrate_name: xyloglucan

substrate: xyloglucan


enzyme_display_name: endo-alpha-sialidase

organism: Phage E

substrate_name: K1 antigen

proof_lines: 37°C, pH 6.5, an alpha-2,8-linked homopolymer of N-acetylneuraminic acid

enzyme: endo-alpha-sialidase

substrate: K1 antigen


organism: Bombyx mori


enzyme_display_name: oligosaccharide reducing-end xylanase

organism: Paenibacillus physcomitrellae

substrate_name: corncob xylan

enzyme: oligosaccharide reducing-end xylanase

substrate: corncob xylan


organism: Bacteroides fragilis


enzyme_display_name: iota-carrageenase

organism: Wenyingzhuangia fucanilytica

substrate_name: iota-carrageenan

proof_lines:
pH 8.0, 30°C, recombinant enzyme
pH 8.0, 25°C

enzyme: iota-carrageenase

substrate: iota-carrageenan


enzyme_display_name: alpha-agarase

organism: Catenovulum agarivorans

substrate_name: agarose

enzyme: alpha-agarase

substrate: agarose


organism: Unknown

substrate_name: Unknown

substrate: Unknown


enzyme_display_name: cellulose 1,4-beta-cellobiosidase (non-reducing end)

organism: Ruminococcus flavefaciens

substrate_name: p-nitrophenyl-beta-D-cellobioside

substrate: p-nitrophenyl-beta-D-cellobioside


organism: Streptomyces coelicolor

substrate_name: cellotetraose

enzyme: cellulose 1,4-beta-cellobiosidase (non-reducing end)

substrate: cellotetraose


organism: Thermothelomyces heterothallicus

substrate_name: p-Nitrophenyl-lactoside

substrate: p-Nitrophenyl-lactoside


organism: Thermotoga petrophila

substrate_name: 4-nitrophenyl beta-D-cellobioside

substrate: 4-nitrophenyl beta-D-cellobioside


enzyme_display_name: alpha,alpha-phosphotrehalase

organism: uncultured bacterium

substrate_name: 4-nitrophenyl alpha-D-glucopyranoside

proof_lines: rTRE_P2, pH and temperature not specified in the publication

proof_lines: rTRE_P3, pH and temperature not specified in the publication

enzyme: alpha,alpha-phosphotrehalase

substrate: 4-nitrophenyl alpha-D-glucopyranoside


enzyme_display_name: NAD+ glycohydrolase

organism: Neurospora crassa

substrate_name: NAD+

enzyme: NAD+ glycohydrolase

substrate: NAD+


organism: Pseudomonas putida


enzyme_display_name: UniProt Q9TTF5

organism: Bos taurus

enzyme: UniProt Q9TTF5


enzyme_display_name: UniProt A4H9Q9

organism: Leishmania braziliensis

substrate_name: adenosine

enzyme: UniProt A4H9Q9

substrate: adenosine


substrate_name: uridine

substrate: uridine


substrate_name: Inosine

substrate: Inosine


substrate_name: cytidine

substrate: cytidine


substrate_name: 4-nitrophenyl beta-D-ribofuranoside

substrate: 4-nitrophenyl beta-D-ribofuranoside


enzyme_display_name: UniProt P33022

organism: Escherichia coli

enzyme: UniProt P33022


enzyme_display_name: UniProt Q97ZS5

organism: Saccharolobus solfataricus

enzyme: UniProt Q97ZS5


enzyme_display_name: ribosylpyrimidine nucleosidase

enzyme: ribosylpyrimidine nucleosidase


enzyme_display_name: UniProt O24915

organism: Helicobacter pylori

substrate_name: S-adenosyl-L-homocysteine

enzyme: UniProt O24915

substrate: S-adenosyl-L-homocysteine


organism: Aspergillus oryzae

substrate_name: dansyl derivative of Taka-amylase A glycopeptide


organism: Ogataea minuta

substrate_name: 9-fluorenylmethyloxycarbonyl-labeled asparagine-linked agalactobiantennary oligosaccharide


organism: Mucor hiemalis

substrate_name: dansyl asialotransferrin glycopeptide

substrate: dansyl asialotransferrin glycopeptide


organism: Vibrio sp.

substrate_name: neoagarobiose

substrate: neoagarobiose


organism: Oryctolagus cuniculus


enzyme_display_name: UniProt A5F5R2

organism: Vibrio cholerae

enzyme: UniProt A5F5R2


enzyme_display_name: UniProt Q8DQ16

organism: Streptococcus pneumoniae

enzyme: UniProt Q8DQ16


enzyme_display_name: UniProt Q99TQ0

organism: Staphylococcus aureus

proof_lines:
Raw SKiD Env: Temp=25°C, pH=7.2
Raw SKiD Env: Temp=-----, pH=7

enzyme: UniProt Q99TQ0


enzyme_display_name: UniProt Q9ZMY2

enzyme: UniProt Q9ZMY2


organism: Acinetobacter sp.


organism: Stigmatella aurantiaca

substrate_name: Unknown


enzyme_display_name: glucan 1,6-alpha-isomaltosidase

organism: Arthrobacter dextranolyticus

substrate_name: Dextran

enzyme: glucan 1,6-alpha-isomaltosidase

substrate: Dextran


organism: Streptomyces griseus


enzyme_display_name: endo-alpha-N-acetylgalactosaminidase

organism: Alcaligenes sp.

substrate_name: dansyl-Ser-GalNAc-Gal

enzyme: endo-alpha-N-acetylgalactosaminidase

substrate: dansyl-Ser-GalNAc-Gal


organism: Brevibacillus laterosporus

substrate_name: asialofetuin

substrate: asialofetuin


enzyme_display_name: glucan 1,4-alpha-maltohexaosidase

organism: Bacillus licheniformis

substrate_name: maltononaose

enzyme: glucan 1,4-alpha-maltohexaosidase

substrate: maltononaose


enzyme_display_name: UniProt Q0PC20

organism: Campylobacter jejuni subsp. jejuni

enzyme: UniProt Q0PC20


enzyme_display_name: arabinan endo-1,5-alpha-L-arabinanase

organism: Caldicellulosiruptor saccharolyticus DSM 8903

enzyme: arabinan endo-1,5-alpha-L-arabinanase


enzyme_display_name: Ipecoside beta-D-glucosidase IpeGLU1

organism: Carapichea ipecacuanha

substrate_name: N-deacetylipecoside

proof_lines: kcat is 28.2 sec(-1) with N-deacetylipecoside as substrate (PubMed:18927081). kcat is 86.3 sec(-1) with N-deacetylisoipecoside as substrate (PubMed:18927081). kcat is 31.1 sec(-1) with 6-O-methyl-N-deacetylipecoside as substrate (PubMed:189

enzyme: Ipecoside beta-D-glucosidase IpeGLU1

substrate: N-deacetylipecoside


substrate_name: N-deacetylisoipecoside

substrate: N-deacetylisoipecoside


substrate_name: ipecoside

substrate: ipecoside


substrate_name: strictosidine

substrate: strictosidine


substrate_name: secologanin

substrate: secologanin


substrate_name: loganin

substrate: loganin


substrate_name: galloyl-glucose

substrate: galloyl-glucose


enzyme_display_name: methylthioadenosine nucleosidase

organism: Unknown

enzyme: methylthioadenosine nucleosidase

substrate: Unknown


organism: Pseudothermotoga thermarum

substrate_name: linear arabinan

substrate: linear arabinan


enzyme_display_name: aminodeoxyfutalosine nucleosidase

organism: Escherichia coli

assay_conditions_summary: pH 7; 310.15 K; buffer 50 mM potassium phosphate

proof_lines: in 50 mM potassium phosphate buffer, pH 7.0, at 37°C

enzyme: aminodeoxyfutalosine nucleosidase


enzyme_display_name: adenosylhomocysteinase

organism: Beta vulgaris

substrate_name: H2O; S-Adenosyl-L-homocysteine

enzyme: adenosylhomocysteinase

substrate: H2O; S-Adenosyl-L-homocysteine


enzyme_display_name: Exoglucanase 2

organism: Hypocrea jecorina

substrate_name: cellotriose

proof_lines: kcat is 0.06 sec(-1) with cellotriose as substrate, 4.1 sec(-1) with cellotetraose as substrate, 1.1 sec(-1) with cellopentaose as substrate and 14 sec(-1) with cellopentaose as substrate.

enzyme: Exoglucanase 2

substrate: cellotriose


substrate_name: cellotetraose

substrate: cellotetraose


substrate_name: cellopentaose

substrate: cellopentaose


organism: Neisseria meningitidis

substrate_name: Unknown


enzyme_display_name: raucaffricine beta-glucosidase

organism: Rauvolfia serpentina

substrate_name: Raucaffricine

column_label: The abstract reports the best substrate specificity for recombinant RG was raucaffricine with KM 1.3 mM.

raw_value_unit_evidence: Km raw_value=1.3 mM raw_unit=mM

row_label: Prose segment: raucaffricine Km

table_label: PubMed PMID 10975500 abstract

source_databases_merged: brenda skid uniprot

enzyme: raucaffricine beta-glucosidase

substrate: Raucaffricine


column_label: The abstract reports strictosidine is a substrate for recombinant RG with KM 1.8 mM.

raw_value_unit_evidence: Km raw_value=1.8 mM raw_unit=mM

row_label: Prose segment: strictosidine Km


organism: Mesocricetus auratus

substrate_name: S-Adenosyl-L-homocysteine; H2O

substrate: S-Adenosyl-L-homocysteine; H2O


enzyme_display_name: UniProt P09960

organism: Homo sapiens

enzyme: UniProt P09960


substrate_name: Arg-Ser-Arg

substrate: Arg-Ser-Arg


enzyme_display_name: UniProt Q6IP81

organism: Xenopus laevis

enzyme: UniProt Q6IP81


enzyme_display_name: UniProt Q9ZAG3

organism: Rhodococcus erythropolis

enzyme: UniProt Q9ZAG3


enzyme_display_name: limonene-1,2-epoxide hydrolase

sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature S15P/M78F to reconstruct the assayed variant sequence.

enzyme: limonene-1,2-epoxide hydrolase


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature S15P/M78F/N92K/F139V to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature S15P/M78F/N92K/F139V/T76K/T85K to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature S15P/M78F/N92K/F139V/T76K/T85K/E45D/I80V/E124D to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature T76K/L114V/I116V to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature T76K/L114V/I116V/N92K/F139V/L147F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature T76K/L114V/I116V/N92K/F139V/L147F/S15D/A19K/L74F/M78F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_accession and applied mutation signature T76K/L114V/I116V/N92K/F139V/L147F/S15D/A19K/L74F/M78F/E45D to reconstruct the assayed variant sequence.


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


enzyme_display_name: uracil-DNA glycosylase

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

enzyme: uracil-DNA glycosylase


enzyme_display_name: Multifunctional glycoside hydrolase

organism: Caldicellulosiruptor owensensis (strain ATCC 700167 / DSM 13100 / OL)

substrate_name: p-nitrophenyl beta-D-galactopyranoside (pNPGal) (at pH 5.5 and 75 degrees Celsius)

proof_lines: kcat is 4522 sec(-1) with pNPGal as substrate. kcat is 3750.6 sec(-1) with pNPGlu as substrate. kcat is 944.3 sec(-1) with pNPC as substrate. kcat is 652.6 sec(-1) with pNPX as substrate. kcat is 2149 sec(-1) with cellobiose as substrate.

enzyme: Multifunctional glycoside hydrolase


substrate_name: p-nitrophenyl beta-D-glucopyranoside (pNPGlu) (at pH 5.5 and 75 degrees Celsius)


substrate_name: p-nitrophenyl beta-D-cellobioside (pNPC) (at pH 5.5 and 75 degrees Celsius)


substrate_name: cellobiose (at pH 5.5 and 75 degrees Celsius)


enzyme_display_name: oligosaccharide reducing-end xylanase

organism: Halalkalibacterium halodurans

substrate_name: D-xylose

sequence_variant_note: Source notes indicate a mutant or variant enzyme, but no machine-applicable substitution signature was available.

enzyme: oligosaccharide reducing-end xylanase

substrate: D-xylose


organism: Paenibacillus barcinonensis

substrate_name: xylotriose

proof_lines: pH 7.0, 40°C, recombinant wild-type enzyme

substrate: xylotriose


enzyme_display_name: UniProt A0A0S2UQQ5

proof_lines: kcat is 118.8 sec(-1) for the hydrolysis of xylotriose.

enzyme: UniProt A0A0S2UQQ5


enzyme_display_name: isochorismatase

organism: Pseudomonas aeruginosa

substrate_name: chorismate

sequence_variant_note: Mutation signature R54K could not be applied safely because the resolved sequence does not match the expected reference residue(s).

enzyme: isochorismatase

substrate: chorismate


sequence_variant_note: Mutation signature Q91N could not be applied safely because the resolved sequence does not match the expected reference residue(s).


column_label: Table One row pKIL10A-Wb, CM activity kcat column.

normalized_value_unit_evidence: WT CM normalized paper kcat=0.085 s^-1.

raw_value_unit_evidence: JBC PDF chrome_pdf_viewer_download.pdf; text lines 313-329 report Table One WT PchBH CM kcat 0.085 +/- 0.011 s^-1.

row_label: pKIL10A-Wb / WT PchBH CM activity

table_label: Table One


substrate_name: isochorismate

substrate: isochorismate


verification_status: corrected

column_label: Table One WT PchBH row, IPL kcat and kcat/Km columns; Km is the exact quotient kcat divided by kcat/Km.

raw_value_unit_evidence: WT PchBH IPL kcat 1.06 s^-1 and IPL kcat/Km 1,130,000 M^-1 s^-1.

row_label: pKIL10A-Wb / WT PchBH; IPL activity

table_label: Table One: Genetic and kinetic characteristics of representative PchB variants

status: corrected


enzyme_display_name: leukotriene-A4 hydrolase

organism: Saccharomyces cerevisiae

substrate_name: L-arginine 2-naphthylamide

sequence_variant_note: Mutation context is present, but no sequence was resolved for this record.

proof_lines: mutant N362L, 25°C, pH not specified in the publication

enzyme: leukotriene-A4 hydrolase

substrate: L-arginine 2-naphthylamide


substrate_name: L-alanine 2-naphthylamide

substrate: L-alanine 2-naphthylamide


substrate_name: L-leucine 2-naphthylamide

substrate: L-leucine 2-naphthylamide


substrate_name: L-methionine 2-naphthylamide

substrate: L-methionine 2-naphthylamide


proof_lines: mutant N362E, 25°C, pH not specified in the publication


substrate_name: L-valine 2-naphthylamide

proof_lines: mutant N362Q, 25°C, pH not specified in the publication

substrate: L-valine 2-naphthylamide


substrate_name: L-isoleucine 2-naphthylamide

substrate: L-isoleucine 2-naphthylamide


sequence_variant_note: No sequence was resolved for this record, so variant-aware sequence handling could not be applied.

proof_lines: wild-type, 25°C, pH not specified in the publication


proof_lines: mutant D399A, 25°C, pH not specified in the publication


proof_lines: mutant W365L, 25°C, pH not specified in the publication


substrate_name: Unknown

substrate: Unknown


enzyme_display_name: lysoplasmalogenase

organism: Thermocrispum sp. RD004668

substrate_name: choline lysoplasmalogen

enzyme: lysoplasmalogenase

substrate: choline lysoplasmalogen


enzyme_display_name: trans-epoxysuccinate hydrolase

organism: Pseudomonas putida

enzyme: trans-epoxysuccinate hydrolase


enzyme_display_name: UniProt G0R7E2

organism: Trichoderma reesei

substrate_name: (R)-styrene oxide

enzyme: UniProt G0R7E2

substrate: (R)-styrene oxide


enzyme_display_name: UniProt P34914

organism: Mus musculus

enzyme: UniProt P34914


proof_lines: pH 7.5, 22°C, enzyme stimulated by leukotriene A4


sequence_variant_note: Resolved canonical sequence from source_record and applied mutation signature D399A to reconstruct the assayed variant sequence.


substrate_name: cis-stilbene oxide

sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature M32L/L35M/L103I/L114M/I116F/F139L to reconstruct the assayed variant sequence.

assay_conditions_summary: pH 8

substrate: cis-stilbene oxide


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature M32L/L35M/M78I/L103I/L114M/I116F to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_rest_accession:Q9ZAG3 and applied mutation signature M32L/L35G/I80W/L103V/F139L to reconstruct the assayed variant sequence.

column_label: Table 3 (Kinetic properties of computationally redesigned epoxide hydrolases), assay-substrate column value '3 a', variant column value '60A', designed-to-produce column value '(R,R)-3 b'; column 'k cat [s-1]' = 0.052+/-0.006; column 'K M [mm]' = 0.06+/-0.01; column 'k cat/K M [M-1 s-1]' = 890. Table 2 lists variant 60A with the mutations M32L_L35G_I80W_L103V_F139L, the export signature M32L/L35G/I80W/L103V/F139L.

normalized_value_unit_evidence: K M: printed Table 3 cell 0.06 in the column headed K M [mm], the paper's lowercase mm for millimolar; no conversion is needed, and the exported value is 0.06 mM. k cat: printed Table 3 cell 0.052 in the column headed k cat [s-1]; the cell is already in s^-1 so no conversion applies, and the exported value is 0.052 s^(-1). k cat/K M: printed Table 3 cell 890 in the column headed k cat/K M [M-1 s-1]; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 890 / 1000 = 0.89 s^-1 mM^-1. The exported ratio is 0.8666666666666667 s^(-1)*mM^(-1), which is the full-precision quotient of the row's own two component cells (0.052 / 0.06 = 0.8666666666666667), and at the figures the paper prints that quotient is 867 M^-1 s^-1, not the printed cell, so the printed efficiency is the paper's own fitted value and kcat_over_km is corrected to it.

raw_value_unit_evidence: Table 3, assay-substrate 3 a block, variant row '60A' (Table 2 mutation list M32L_L35G_I80W_L103V_F139L): k cat = 0.052 s-1; K M = 0.06 mm; printed k cat/K M = 890 M-1 s-1.

table_label: Table 3: Kinetic properties of computationally redesigned epoxide hydrolases (Chembiochem 2020, 21(13):1893-1904)


substrate_name: cyclopentene oxide

substrate: cyclopentene oxide


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature L35W/L74F/I80G/I116V/F139L to reconstruct the assayed variant sequence.


column_label: Table 3 (Kinetic properties of computationally redesigned epoxide hydrolases), assay-substrate column value '3 a', variant column value 'LEH-P', designed-to-produce column value '-'; column 'k cat [s-1]' = 0.147+/-0.012; column 'K M [mm]' = 0.37+/-0.02; column 'k cat/K M [M-1 s-1]' = 406.

normalized_value_unit_evidence: K M: printed Table 3 cell 0.37 in the column headed K M [mm], the paper's lowercase mm for millimolar; no conversion is needed, and the exported value is 0.37 mM. k cat: printed Table 3 cell 0.147 in the column headed k cat [s-1]; the cell is already in s^-1 so no conversion applies, and the exported value is 0.147 s^(-1). k cat/K M: printed Table 3 cell 406 in the column headed k cat/K M [M-1 s-1]; 1 M^-1 s^-1 is 0.001 s^-1 mM^-1, so 406 / 1000 = 0.406 s^-1 mM^-1. The exported ratio is 0.3972972972972973 s^(-1)*mM^(-1), which is the full-precision quotient of the row's own two component cells (0.147 / 0.37 = 0.3972972972972973), and at the figures the paper prints that quotient is 397 M^-1 s^-1, not the printed cell, so the printed efficiency is the paper's own fitted value and kcat_over_km is corrected to it.

raw_value_unit_evidence: Table 3, assay-substrate 3 a block, reference row 'LEH-P': k cat = 0.147 s-1; K M = 0.37 mm; printed k cat/K M = 406 M-1 s-1.


substrate_name: leukotriene A4

proof_lines: pH 7.5, 22°C, 5S,6S-dihydroxy-7,9-trans-11,14-cis-eicosatetraenoic acid production

substrate: leukotriene A4


enzyme_display_name: microsomal epoxide hydrolase

organism: Trypanosoma cruzi

substrate_name: styrene oxide

enzyme: microsomal epoxide hydrolase

substrate: styrene oxide


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_consensus_sequence and applied mutation signature F375Y to reconstruct the assayed variant sequence.


substrate_name: Raucaffricine

proof_lines: wild type enzyme, pH and temperature not specified in the publication

substrate: Raucaffricine


substrate_name: strictosidine

substrate: strictosidine


enzyme_display_name: cellulose 1,4-beta-cellobiosidase (non-reducing end)

organism: Cellulomonas fimi

substrate_name: 4-nitrophenyl beta-D-cellobioside

substrate: 4-nitrophenyl beta-D-cellobioside


enzyme: cellulose 1,4-beta-cellobiosidase (non-reducing end)


proof_lines: pH and temperature not specified in the publication


enzyme_display_name: Aminodeoxyfutalosine nucleosidase

organism: Helicobacter pylori (strain J99 / ATCC 700824)

proof_lines: kcat is 12.1 sec(-1) with 5'-methylthioadenosine as substrate, and 4.3 sec(-1) with aminodeoxyfutalosine as substrate (PubMed:22891633). kcat is 4.92 sec(-1) with 5'-methylthioadenosine as substrate (at pH 7.5 and 37 degrees Celsius) (PubMe

enzyme: Aminodeoxyfutalosine nucleosidase


substrate_name: aminodeoxyfutalosine

substrate: aminodeoxyfutalosine


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature E296Q with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature E296D with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


sequence_variant_note: Resolved canonical sequence from uniprot_ec_organism_unique and applied mutation signature E296N with inferred residue-numbering offset +1 to reconstruct the assayed variant sequence.


organism: Catharanthus roseus

proof_lines:
enzyme II
enzyme I
pH 6.0, 37°C


enzyme_display_name: Exoglucanase GH7B

organism: Limnoria quadripunctata

proof_lines: kcat is 10.57 min(-1) for p-nitrophenyl-beta-d-cellotrioside (pNP-G3). kcat is 24.42 min(-1) for p-nitrophenyl-beta-d-cellopentaoside (pNP-G5).

enzyme: Exoglucanase GH7B


enzyme_display_name: Endoglycoceramidase I

organism: Rhodococcus hoagii (strain 103S)

substrate_name: monosialotetrahexosylganglioside (GM1)

proof_lines: kcat is 10.5 sec(-1) with monosialotetrahexosylganglioside as substrate.

enzyme: Endoglycoceramidase I

substrate: monosialotetrahexosylganglioside (GM1)


enzyme_display_name: beta-apiosyl-beta-glucosidase

organism: Dalbergia nigrescens

source_databases_merged: brenda uniprot

enzyme: beta-apiosyl-beta-glucosidase


organism: Caldicellulosiruptor saccharolyticus

proof_lines: domain construct CBM3-GH5, at pH 5.5 and 80°C,


enzyme_display_name: Funoran endo-alpha-hydrolase

organism: Aquimarina sp. (strain AD1)

substrate_name: funoran

smiles: Cl

proof_lines: kcat is 43.40 sec(-1) with funoran as substrate. kcat is 101.39 sec(-1) with agarose as substrate.

enzyme: Funoran endo-alpha-hydrolase

substrate: funoran


organism: Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)

proof_lines: kcat is 0.53 sec(-1) with aminodeoxyfutalosine as substrate. kcat is 2.7 sec(-1) with 5'-methylthioadenosine as substrate.


enzyme_display_name: aminodeoxyfutalosine nucleosidase

organism: Campylobacter jejuni subsp. jejuni serotype O:2

column_label: Table 1. Substrate Specificity and Kinetic Parameters for CjMTAN; row 5'-methylthioadenosine; columns kcat, Km, kcat/Km; kcat raw_value=0.80 +/- 0.02 raw_unit=s^-1; Km raw_value=7.1 +/- 0.7 raw_unit=uM; kcat/Km raw_value=11.3 (+/-1.1) x 10^4 raw_unit=M^-1 s^-1

normalized_value_unit_evidence: kcat normalized_value=0.8 normalized_unit=s^-1; Km normalized_value=0.0071 normalized_unit=mM; kcat/Km normalized_value=113.0 normalized_unit=s^-1*mM^-1

enzyme: aminodeoxyfutalosine nucleosidase


column_label: Table 1. Substrate Specificity and Kinetic Parameters for CjMTAN; row 5'-methylthioadenosine; columns Km; Km raw_value=7.1 +/- 0.7 raw_unit=uM

raw_value_unit_evidence: Km raw_value=7.1 +/- 0.7 raw_unit=uM


enzyme_display_name: Enterobactin synthase component B

organism: Escherichia coli (strain K12)

substrate_name: isochorismate (at pH 7 and 37 degrees Celsius)

column_label: PubMed abstract metric sentence gives Km for isochorismate as 14.7 microM, normalized to 0.0147 mM

normalized_value_unit_evidence: PubMed abstract Km normalized_value=0.0147 normalized_unit=mM

raw_value_unit_evidence: PubMed abstract: The enzyme has a Km for isochorismate of 14.7 microM and a turnover number of 600 min-1

row_label: EntB isochorismatase Km sentence

table_label: PubMed abstract

enzyme: Enterobactin synthase component B


enzyme_display_name: maltose-6'-phosphate glucosidase

organism: Klebsiella pneumoniae

substrate_name: Trehalulose 6'-phosphate; H2O

source_license: CC BY 4.0; SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

source_databases_merged: sabio_rk skid uniprot

enzyme: maltose-6'-phosphate glucosidase

substrate: Trehalulose 6'-phosphate; H2O


enzyme_display_name: endoglycosylceramidase

organism: Cyanea nozakii

source_databases_merged: sabio_rk brenda uniprot

enzyme: endoglycosylceramidase


enzyme_display_name: raucaffricine beta-glucosidase

organism: Rauvolfia serpentina

enzyme: raucaffricine beta-glucosidase


enzyme_display_name: Coniferin beta-glucosidase

organism: Pinus contorta

substrate_name: 4-methylumbelliferyl p-glucoside

enzyme: Coniferin beta-glucosidase

substrate: 4-methylumbelliferyl p-glucoside


enzyme_display_name: Tail spike protein

organism: Escherichia phage K1E

substrate_name: polysialic acid

enzyme: Tail spike protein

substrate: polysialic acid


organism: Trichoderma reesei

substrate_name: carboxymethylcellulose

substrate: carboxymethylcellulose


enzyme_display_name: leucyl aminopeptidase

organism: Thermotoga maritima

enzyme: leucyl aminopeptidase


organism: Fibrobacter succinogenes

substrate_name: p-nitrophenyl-beta-D-cellobioside

proof_lines: extracellular periplasmic

substrate: p-nitrophenyl-beta-D-cellobioside


organism: Flavobacterium sp.


organism: Streptomyces plicatus


enzyme_display_name: endo-alpha-N-acetylgalactosaminidase

organism: Bifidobacterium bifidum

substrate_name: H2O; GalNAc-alpha1-Ser

source_license: SABIO-RK terms (free for academic use; see sabiork.h-its.org); CC BY-NC-ND 4.0

enzyme: endo-alpha-N-acetylgalactosaminidase

substrate: H2O; GalNAc-alpha1-Ser


enzyme_display_name: Endo-alpha-N-acetylgalactosaminidase

organism: Streptococcus pneumoniae (strain ATCC BAA-255 / R6)

enzyme: Endo-alpha-N-acetylgalactosaminidase


organism: Aspergillus aculeatus

substrate_name: AZCL-debranched arabinan

substrate: AZCL-debranched arabinan


enzyme_display_name: arabinan endo-1,5-alpha-L-arabinanase

organism: Bacillus subtilis

substrate_name: Arabinotetraose; H2O

enzyme: arabinan endo-1,5-alpha-L-arabinanase

substrate: Arabinotetraose; H2O


enzyme_display_name: mannan endo-1,6-alpha-mannosidase

organism: Niallia circulans

enzyme: mannan endo-1,6-alpha-mannosidase


organism: Catharanthus roseus


organism: Clostridium acetobutylicum


substrate_name: H2O; Trehalulose 6'-phosphate

substrate: H2O; Trehalulose 6'-phosphate


organism: Fusobacterium mortiferum

substrate_name: Maltose 6'-phosphate; H2O

substrate: Maltose 6'-phosphate; H2O


organism: Bacillus subtilis (strain 168)


organism: Campylobacter jejuni subsp. jejuni serotype O:2

column_label: Table 1. Substrate Specificity and Kinetic Parameters for CjMTAN; row S-5'-Adenosyl-L-homocysteine; columns kcat, Km; kcat raw_value=0.55 +/- 0.03 raw_unit=s^-1; Km raw_value=12.2 +/- 1.5 raw_unit=uM

normalized_value_unit_evidence: kcat normalized_value=0.55 normalized_unit=s^-1; Km normalized_value=0.0122 normalized_unit=mM


enzyme_display_name: adenosylhomocysteinase

organism: Homo sapiens

substrate_name: Homocysteine; Adenosine

enzyme: adenosylhomocysteinase

substrate: Homocysteine; Adenosine


organism: Rattus norvegicus

substrate_name: H2O; S-Adenosyl-L-homocysteine

substrate: H2O; S-Adenosyl-L-homocysteine


substrate_name: S-Adenosyl-L-homocysteine; H2O

substrate: S-Adenosyl-L-homocysteine; H2O


enzyme_display_name: Lysoplasmalogenase TMEM86B

substrate_name: lysoplasmenyl-choline

enzyme: Lysoplasmalogenase TMEM86B

substrate: lysoplasmenyl-choline


enzyme_display_name: lysoplasmalogenase

organism: Mycobacterium tuberculosis

substrate_name: choline lysoplasmalogen

enzyme: lysoplasmalogenase

substrate: choline lysoplasmalogen


enzyme_display_name: leukotriene-A4 hydrolase

substrate_name: H2O; L-Leucinyl-p-nitroanilide

enzyme: leukotriene-A4 hydrolase

substrate: H2O; L-Leucinyl-p-nitroanilide


organism: Mus musculus

substrate_name: Leukotriene A4; H2O

substrate: Leukotriene A4; H2O


organism: Saccharomyces cerevisiae


enzyme_display_name: Leukotriene A-4 hydrolase

organism: Xenopus laevis

substrate_name: LTA4 at room temperature, pH 7.8

enzyme: Leukotriene A-4 hydrolase

substrate: LTA4 at room temperature, pH 7.8


organism: Drosophila melanogaster

substrate_name: 5-fluoro-beta-L-gulosyl fluoride

substrate: 5-fluoro-beta-L-gulosyl fluoride


substrate_name: 2,4-dinitrophenyl alpha-D-mannoside

sequence_variant_note: Resolved canonical sequence from identity matching and applied mutation signature D341N to reconstruct the assayed variant sequence.

substrate: 2,4-dinitrophenyl alpha-D-mannoside


sequence_variant_note: Sequence is treated as wild-type because the source explicitly marked the record as wild-type.


organism: Rhodococcus sp. M-777

sequence_variant_note: Sequence identity was resolved, but no explicit wild-type or mutant call was captured.

proof_lines: from horse erythrocyte cell surface, pH 7, 37°C

proof_lines: i.e. Gg3Cer from guinea pig erythrocyte cell surface, pH 7, 37°C